LOCUS       DATIRT010000001         4095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_688290, whole genome shotgun sequence.
ACCESSION   DATIRT010000001 DATIRT010000000
VERSION     DATIRT010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688290"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /gene="mltB"
                     /locus_tag="VLN25_00005"
     CDS             <1..1064
                     /gene="mltB"
                     /locus_tag="VLN25_00005"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HSQ08990.1"
                     /translation="SAAQGHPRLQATPEPAVTMRRRTALLTLLALAAAPVVHARTPYL
                     ARSEVQQFLNELVSSHGFERARAEHWFREARYSPSVERLMQPAIPFGQRNWFEYRDRY
                     VEPVRIQAGIAFWQAHAAALARCEELLGVPAEILVAIIGIETYFGRITGNFRTLDVFT
                     TLSFDYLRRADFYRAEFVEFLLLARDQKRDPGTYRSSFAGAIGLPQFMPGSIRRWAVD
                     FDGDGRIDLLGSPVDAIGSVASFLVGHGWQRDLPIAFEVEAEEGIVDRLGRGILAQRP
                     WWSALAEGVTGDALLTLDTPVLVIDLPLVTPDGQPGRAYRVGTVNFSAILHYNRSYFY
                     ATAVTEFAAALRERMNSGS"
     gene            complement(1096..1740)
                     /gene="maiA"
                     /locus_tag="VLN25_00010"
     CDS             complement(1096..1740)
                     /gene="maiA"
                     /locus_tag="VLN25_00010"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HSQ08991.1"
                     /translation="MKLYNYFRSSASFRVRIALNLKRLSYDYAVVHLTKGGGQQFAPA
                     FRALNADALIPVLDDDGHVLTQSLAIIEYLDETHPQPPLLPQAPLDRAYVRACALTIA
                     CEIHPLNNLRVLRYLVQQLGASEDQKNAWYRHWVEQGLAAMEARVAGERRYGRFMLSD
                     TVTLADVALVPQIFNARRFDCRLDHVPVLMGIFDRCMELAAFIDAQPARQPDAE"
     gene            complement(1753..3072)
                     /locus_tag="VLN25_00015"
     CDS             complement(1753..3072)
                     /locus_tag="VLN25_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HSQ08992.1"
                     /translation="MSTWAPLFLFAAMLALMALRVPIWVAMFVPGAIGYIGMSGMEPW
                     LANLKGMAFARFSIYDLSVIPLFLLMGQLATHGGLSRSLFRAATSFVGHWRGGLAQAA
                     VLASAAFGSVCGSSVATAATIAQVALPEMRRYRYDSGFAVATLAAGGTLGILIPPSVV
                     LVLYAIVAEQNIAKLFAAALLPGILAAALYCIAIALLMRWRPALGPAEPRVPWIERWS
                     TLAGVLPVLAIFIIVLGGIYGGVFTPTEAAAVGVALTGLAGLLRGELKRAGLRAALLA
                     TAETSGVIFMIFLGADMLNGALALTQMPMRAAAFVSELPLPPLAVLALVLVFYIVLGG
                     VMDELSMLLLTLPVVFPAVMQLDLLGLPLEAKAIWFGVLLLTVVNIGLIAPPIGLNVH
                     VVGALARDVPIARAYRHVGAFLGADALRLALLLLAPPVSLWLVQFVR"
     gene            complement(3072..3545)
                     /locus_tag="VLN25_00020"
     CDS             complement(3072..3545)
                     /locus_tag="VLN25_00020"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HSQ08993.1"
                     /translation="MGRALRAAAVAFAMAGGGVLLLVAIVTLVSVGGRWLLAQPVAGD
                     VELVQLGTATALALFLPYCQLHGSHLIVDIFTARAGSSVHRWLDFAAHAIAAVVLALL
                     ALRAGAGIVDLYRAGETSMVLGLPLWMAYAAMVPSLGLAAFIALLGSQRDEPDNQ"
     gene            complement(3548..>4095)
                     /locus_tag="VLN25_00025"
     CDS             complement(3548..>4095)
                     /locus_tag="VLN25_00025"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HSQ08994.1"
                     /translation="NKLLSALGAAPVSMPVTGLADAMSKGVLDGAVVPWEVVPAVKVH
                     EVARFHTETDPNVPALYTAVFLFAMNAKTYAGLPADLRAVIDRNSGVGLSTQAGRLWD
                     EQTAPARKLALDRGNTIIVVPASELARWQRAAQSVQSEWIVDMNRRQLDGNALLADAR
                     ALLARSAAATALGAPRERTSK"
BASE COUNT          774 a         1390 c         1318 g          613 t
ORIGIN      
        1 cgagcgctgc gcaaggccat ccgcgccttc aagccacgcc cgaacccgct gtgaccatgc
       61 ggcgccgaac cgccctgctt acgctgctgg cgctggcagc cgcacccgtc gtgcatgccc
      121 gcaccccata tctcgcacgc agtgaagttc agcagttcct caacgaactg gtgagctctc
      181 acgggttcga acgggcccgt gccgagcact ggttcaggga agcgcgctat tcgccaagcg
      241 tcgaacggct gatgcagcct gcgatcccct tcggccagcg caattggttc gagtatcgcg
      301 accgatatgt cgagccggta cggatacagg ctggcatcgc gttctggcag gcgcacgccg
      361 cagcactggc gcggtgcgaa gagctgcttg gcgtgccggc cgagatcctc gtcgccatca
      421 tcggtatcga aacctacttc ggacgcatca ctggaaactt ccgcacgctc gacgtcttta
      481 cgacgctgag cttcgattac ctgcgtcgcg ccgacttcta tcgagccgag ttcgtcgagt
      541 tcctgctgct ggcgcgcgac cagaaacggg atccaggcac gtaccgtagt tcgttcgccg
      601 gcgcgatcgg cctgccccag ttcatgcctg gttcgatccg gcgctgggcg gtggatttcg
      661 acggtgacgg ccgcatcgac cttttgggaa gcccggtcga cgcgatcggc tcggtcgcga
      721 gtttcctggt cgggcacggc tggcagcgcg atctgcccat cgccttcgag gtcgaagccg
      781 aagaaggcat tgtcgaccga ctcggccgcg gcatcctggc gcaacggccc tggtggtccg
      841 cgctggccga aggcgtcacc ggcgatgcat tgctgacact cgacacgccg gtactcgtga
      901 tcgacctgcc gctggtgaca ccggacgggc agcctggacg cgcgtaccgg gttggcacgg
      961 tcaacttcag tgcgatcctg cactacaacc gcagctactt ctatgcgacc gccgtgaccg
     1021 aattcgcggc cgcgcttcgc gagcgaatga actccggctc ctgaacaaag gagcaactcg
     1081 ggagcgagcg gcctgctatt cggcgtcggg ctgccgcgcc ggctgcgcat cgatgaacgc
     1141 cgccagttcc atgcagcggt cgaaaatgcc catcagcacg ggaacgtggt cgaggcgaca
     1201 gtcaaagcgt cgcgcgttga agatctgcgg caccagcgcg acgtcggcca gggtgactgt
     1261 gtcgctcagc atgaagcggc cgtagcggcg ttcgcccgcg acgcgcgcct ccatcgcggc
     1321 cagtccctgt tcgacccagt gccggtacca cgcatttttc tggtcttcac ttgcgccaag
     1381 ctgctgcacc aggtagcgca gtacccgcag gttgttcagc ggatggatct cgcaggcaat
     1441 cgtcagcgcg caggcacgca cgtaggcccg gtcgagcgga gcctgaggca gcagcggtgg
     1501 ttgcggatgc gtttcgtcga ggtactcgat gatcgccagc gactgtgtca gtacatggcc
     1561 gtcatcgtcg agcaccggga tcagcgcgtc ggcgttgagc gcacggaagg ccggggcgaa
     1621 ctgctgcccg cctcctttgg tcaggtgaac caccgcatag tcatagctca atcgcttcag
     1681 gttgagcgcg atgcggacgc ggaacgacgc cgagctgcga aagtagttat agagcttcag
     1741 catgaggaga tcctagcgta cgaactgcac aagccacagc gacacgggcg gtgccagcag
     1801 cagcagcgcc aggcgcagcg cgtcggcgcc gagaaacgca ccgacgtgac ggtaggcgcg
     1861 tgcaatcggc acgtcgcgcg ccagcgcacc aaccacgtga acattcagac cgatcggcgg
     1921 cgcgatcagt ccgatgttga ccacggtcag cagcaacaca ccgaaccaga tggcctttgc
     1981 ctcgagcggt aggcccaaca gatcgagctg catcacggcc gggaacacca ctggcagcgt
     2041 cagcagcaac atcgacagtt cgtccatcac gccgcccagc acgatgtaga acacgagtac
     2101 gagcgcaagg acagcgagtg gcggcagcgg cagttcactg acgaacgcag ctgcgcgcat
     2161 cggcatctgc gtcagcgcga gcgcaccgtt aagcatgtcc gcgccgagga agatcatgaa
     2221 gatcaccccc gacgtctccg cggttgccag cagagcagcg cgcaacccgg cgcgcttcag
     2281 ttctccgcgc agtaggccgg ccagtccggt caacgccacg ccgacggcgg cagcttcggt
     2341 cggggtgaac acacccccgt agatcccgcc caggacaatg atgaagatcg ccagcaccgg
     2401 caacacaccg gcgagagtgg accatcgctc gatccagggc acgcgcggtt ctgccggtcc
     2461 gagcgccgga cgccagcgca tcagcagcgc gatcgcgatg cagtacaggg cggcggcgag
     2521 gatgcctggc aagagcgccg cggcgaagag cttggcgatg ttctgttcgg caacgatcgc
     2581 gtagagcacc agcaccaccg acggcgggat caggatgccg agcgttcctc ccgccgccag
     2641 cgtcgccacc gcgaagccgc tgtcgtagcg ataccgtcgc atctcgggca gcgcaacctg
     2701 cgcaatggtg gccgccgttg ccaccgacga accgcagacc gagccgaacg ccgcactcgc
     2761 cagcacagct gcctgcgcaa gcccgccgcg ccagtggccg acgaaactcg tcgcggctcg
     2821 aaacagcgac cgggaaaggc ctccgtgagt cgcaagctga cccatcaaca ggaacagcgg
     2881 gatcactgac aggtcgtaga tcgaaaagcg cgcgaaggcc atgcccttca ggttggcgag
     2941 ccagggctcc atgcccgaca tcccgatgta gccgatcgcg cccggcacga acatggccac
     3001 ccagatcggc acccgcaagg ccatcagcgc cagcatcgcg gcgaagagaa aaagcggagc
     3061 ccaggtcgac atcactgatt gtccggctca tcgcgctgcg aaccgagcag tgcgataaac
     3121 gcggcgaggc ccagcgacgg caccatcgcc gcgtaagcca tccacagcgg caggccaagc
     3181 accatcgatg tctcgccggc gcggtacagg tcgacgatgc cggctccggc gcgcagggcg
     3241 agcagcgcga gcaccacggc tgcgatggca tgcgctgcaa agtcgagcca ccgatgcacc
     3301 gatgatcccg cacgcgcggt gaagatgtcg acgatcagat ggctgccatg cagctggcag
     3361 tagggaagaa agagcgccag tgcggtggcc gtgcccagct gcactagttc gacgtccccg
     3421 gccacgggct gagccagcag ccagcgcccg ccgacggaaa ccagggtgac gatcgccacc
     3481 agcagcagca ccccgccgcc ggccatcgca aacgccacgg ccgcggcgcg tagcgcgcgc
     3541 cccatgatta cttcgaagtc cgctcgcgtg gtgcgcccag tgccgtggcc gcggcactgc
     3601 gcgccagcag cgcacgcgca tcggcaagca gcgcgttgcc gtcgagttgc cggcggttca
     3661 tgtcgacgat ccactcggac tgcactgact gggccgcgcg ctgccatcgc gccagctcgg
     3721 acgcgggaac gacgatgatg gtgttgccgc gatcgagcgc cagcttgcgc gccggcgctg
     3781 tctgttcgtc ccagaggcgc cccgcctgcg tggaaagtcc cactcccgag ttgcgatcaa
     3841 tgaccgctcg caggtccgcg ggcagacccg cgtaggtctt cgcgttcatc gcaaacagga
     3901 ataccgccgt gtacagcgcg ggcacgttgg ggtcggtttc ggtgtggaag cgcgccacct
     3961 cgtggacctt gacggcgggt accacttccc acggcaccac ggcgccgtcc agtacgccct
     4021 tgctcattgc gtcagccagc ccggtgactg gcatcgacac cggcgcggct cccagcgccg
     4081 acagcagctt gttcg
//
LOCUS       DATIRT010000002         2172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_295023, whole genome shotgun sequence.
ACCESSION   DATIRT010000002 DATIRT010000000
VERSION     DATIRT010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295023"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..147)
                     /locus_tag="VLN25_00030"
     CDS             complement(<1..147)
                     /locus_tag="VLN25_00030"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HSQ08995.1"
                     /translation="MARAIPNILTIAGVDPSGGAGSLADVKTISALGGYACAVITALT
                     AQNTR"
     gene            319..966
                     /locus_tag="VLN25_00035"
     CDS             319..966
                     /locus_tag="VLN25_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSQ08996.1"
                     /translation="MRSSPESTAISKPRWERRKESRPAELVASALELFVEKGYAGTRL
                     DDVAAHAGVSKGTLYLYFENKEDLFKAVVRDNVVARISETAGEVMRFDGSSGELLRFL
                     VSGWWCDYGDSKAGGLGKLIMAESGNFPEIARFFVEEVIEPWHDVLAAAISRGIQSGE
                     FRPVDSRMFVRVMSAPLVMLSLWNRSFGPCSQQPVDAEAYLATHIDMVLSALRKG"
     gene            1198..1866
                     /locus_tag="VLN25_00040"
     CDS             1198..1866
                     /locus_tag="VLN25_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HSQ08997.1"
                     /translation="MDFEKARFNMVEQQVRPWDVLDQTVLDLLLVVKREEFVPPEYRA
                     LAFSDMEIPLRVDGVDTGEAMWSPKLEARILQELALKLHENVLEIGTGSGYFAALLAH
                     KARHVVSVEIDPRLQAFGTRNLARSGVHNVRLESGDASRGWSGRAPYDVIVATGSLPV
                     VPAALRNQLKIGGRLAAVVGTDPVMSAQIITRVSEAGFDTVKLFETRLAPLRNAERPS
                     SFRF"
     gene            1866..>2172
                     /locus_tag="VLN25_00045"
     CDS             1866..>2172
                     /locus_tag="VLN25_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HSQ08998.1"
                     /translation="MKSVSASELSAWLADEGRAAPVLLDVREPWETQICRIEPSELVP
                     MRALPGKVHELDTTRPIVCVCHHGGRSMHVAMFLARQGFENVYNLTGGVDAWARQVDA
                     "
BASE COUNT          382 a          686 c          702 g          402 t
ORIGIN      
        1 acgcgtgttc tgagccgtca gcgcagtgat caccgcgcag gcatagcctc cgagcgcgct
       61 gattgtcttt acgtcggcga gacttcccgc gcctccggat ggatcgacgc cggcaatggt
      121 gagaatgttg ggaatggccc gagccatcag aaggatcgcc aagttcgcct gacctgccgc
      181 tagcttgaca cggctcgaga gctacccgta tattactgac cggtcagtta cttaaaggct
      241 tgcctgcgca aggggaccgc gagtgccgcg cccctccctc cgatcggtgt tcgtgtcccc
      301 ttgcgccggc tcctccgcat gagaagttcc ccagaatcga ccgcgatctc caaaccccgc
      361 tgggaacgcc gcaaggaatc acggcccgcc gaactggtgg cctccgcact cgaactgttc
      421 gtcgagaagg gctacgccgg cacccgcctc gacgatgtcg ctgcgcatgc gggcgtttcc
      481 aagggcacgc tttacctgta tttcgagaac aaggaagact tgttcaaggc ggtcgtgcgc
      541 gacaacgtcg tcgcacgcat cagcgaaacc gccggcgaag tcatgcgctt tgatgggtcg
      601 agcggtgagc tgctgcgctt cctggtctcg ggctggtggt gcgactacgg tgacagcaag
      661 gccggcggcc tcggcaagct gatcatggcg gaaagcggca acttcccgga gatcgcgcgc
      721 ttcttcgtcg aagaagtcat cgagccttgg cacgacgtgc tcgccgctgc gatcagccgc
      781 ggcatccaga gtggcgagtt ccgcccggtt gattccagga tgttcgtgcg cgtgatgtcg
      841 gcgccgctcg tgatgctgtc cctgtggaat cgctcgtttg gcccctgcag ccagcagccg
      901 gtcgatgccg aagcctatct cgccactcat atcgatatgg tgctatccgc gctgcgcaag
      961 ggttgatcct gccgggacgt gccgagcgaa gaggccatgc gttcccgtcg aagtgatccg
     1021 cgtgcgaatg cgggcgcgtc gaaagtagtt tccgaggtca acgtgcgttg gatgcgacag
     1081 accgcatgat tcgggggtcg aaccgcagca gaacagcaaa gaggcgctga tagactttcg
     1141 cgctgttgtg ccggggccct gtaccccggt cacgcaatgg gacccgcaat gcagtcgatg
     1201 gatttcgaga aagcgcgctt caacatggtc gagcagcagg tgcggccatg ggatgtgctc
     1261 gatcagaccg tgctggacct gctgctcgtg gtcaagcgcg aggagttcgt tccaccggag
     1321 taccgcgccc tggcgttttc ggatatggaa atcccgctgc gcgtcgacgg cgtcgacaca
     1381 ggcgaggcca tgtggtcacc gaagctggag gcccgcatcc tgcaggaact cgcactcaag
     1441 ctgcacgaaa acgtgctgga gatcggtacc ggctccggat actttgccgc cctgctcgcc
     1501 cacaaggcgc ggcatgtggt ctcggtcgaa atcgacccgc gcttgcaggc gttcggcacg
     1561 cgcaatctgg cgcgatccgg cgtgcacaac gtcaggctcg aatccggcga cgccagccgc
     1621 ggctggagcg ggcgcgcacc ctacgatgtc atcgtcgcga cgggttcgtt gcccgtcgtg
     1681 ccggcggcac ttcgcaacca actgaagatc ggcgggcgac tggccgccgt ggtcggaacc
     1741 gacccggtga tgtccgcaca gatcatcacg cgcgtgtcgg aagccggctt tgataccgtc
     1801 aagctcttcg agacccgact tgcgcccctg cgcaatgccg agaggccgtc gagcttccgg
     1861 ttctgatgaa gtcggtgtcc gcatccgaac tgtccgcatg gctcgccgac gaaggtcgtg
     1921 cagcgcccgt gctgctcgac gtgcgcgagc cgtgggaaac acagatctgc cgcatcgagc
     1981 cctccgagct ggtgccgatg cgcgctctgc ctggcaaggt gcatgaactg gataccacgc
     2041 gccccatcgt ctgcgtctgc catcacggcg gccgcagcat gcacgtggcg atgttcctgg
     2101 cgcggcaggg attcgagaac gtctacaacc tgactggcgg cgtcgatgcc tgggcacgcc
     2161 aggtcgatgc cg
//
LOCUS       DATIRT010000003         2598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_295098, whole genome shotgun sequence.
ACCESSION   DATIRT010000003 DATIRT010000000
VERSION     DATIRT010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295098"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VLN25_00050"
     CDS             <1..292
                     /locus_tag="VLN25_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSQ08999.1"
                     /translation="TARARTDDIALKLYYDDSDFAVLEALKGVAQAKGVTNATLAYAW
                     LLSRPGVTAPIVGASQTWQLEEAAKALEVTLTPEDRTALEAPYRPHPILGHS"
     gene            complement(306..704)
                     /locus_tag="VLN25_00055"
     CDS             complement(306..704)
                     /locus_tag="VLN25_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09000.1"
                     /translation="MHGTKLTACMAALVAATTLSACAPDAWQNTKATGFNDYLKTVEA
                     QCQPLWIGQMNLRSFDASSAGGQVGNFDMLLDSTSRLYYNRITPAAFRNSVQAMVTTS
                     TDARTNQSIDCMIAKLPPERPRSPPGAVPY"
     gene            complement(881..1273)
                     /locus_tag="VLN25_00060"
                     /pseudo
     CDS             complement(881..1273)
                     /locus_tag="VLN25_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            1549..2334
                     /locus_tag="VLN25_00065"
     CDS             1549..2334
                     /locus_tag="VLN25_00065"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HSQ09001.1"
                     /translation="MDLTEPNTVGLNTLNRPTGPATLGPITVPTVVGAVSMASADRSH
                     VSREIASWRQLKMWLDGESLQSAVTVKLIGVHEGKSLLVTSPDETQLYLREGRLYRFR
                     SFSGEFVYEFVAPLIKVCTAPFPYLHIGWAGSRQVEKRNLRAARRVRTDLQCMVYPGQ
                     QASGRFAKGLIHDLSTAGAAITLQDELAVFYDEVKVVFRVQVAEQEILIEARARAVRK
                     PEAGAPDPTLGVAFVGLGDLERLALHAYVNSSLVQELELPLYA"
BASE COUNT          428 a          844 c          857 g          469 t
ORIGIN      
        1 cacggcccgt gctcgaaccg acgatatcgc cctcaagctc tactacgacg acagcgattt
       61 cgccgtcctc gaggcgctca agggcgtggc gcaggccaag ggtgtgacga atgcgacgct
      121 ggcgtacgcg tggctgctgt caaggcccgg cgtgacagcg ccgatcgtcg gcgccagcca
      181 gacctggcaa ctggaggagg cggccaaggc actggaggtg acgctgactc cggaagaccg
      241 cacggcgctc gaggcaccgt atcggccgca cccgatactc ggacacagct gaaagctgca
      301 tttgctcagt agggcacggc gcctggcggg ctgcgcgggc gctccggcgg cagcttggcg
      361 atcatgcagt cgatggactg gttcgtgcgc gcgtccgtcg aggtcgtcac cattgcctgc
      421 actgaattgc ggaaggcggc cggggtgatc cggttgtagt aaagccgcga cgtcgaatcc
      481 agcagcatgt cgaagttgcc aacctgtccg ccagccgatg acgcatcgaa gctgcgcagg
      541 ttcatctgcc cgatccacag cggctgacac tgcgcctcga ctgtcttgag gtaatcgttg
      601 aagccggtcg ccttggtgtt ctgccaggca tccggggcac acgcggaaag tgttgttgca
      661 gccaccagcg cggccatgca ggccgtgagc ttcgtaccgt gcaccgtgat ctccccggcc
      721 ccgtcggcag aaccagcgca tcgattgtat cgaggcgtgg ggccgatccg cccgttcagg
      781 cgttgcgcgg cgtgggcgtc ggtcgcagca ggggcactgc gatcttgggc gcaggcgcgg
      841 atggggcgcg cgcctcaccg caagagggcc atgccgacga tcgcagcagg atgttgcgca
      901 ccttttcctc gagcgtcgac ttggtcaacg gcttcaggat cgtgccattg gctccgagcc
      961 gaatcgcctg caggacatcg tccttgctgg cctcgttggt gatcagcaag accggcagat
     1021 ccttcagcgt agcgtcggca cggatctgcc gcagcagttc gtagccgccg atgcgcggca
     1081 tgttgatgtc gctgataacg aagtcgtagg ggctctggcg caacatttcc agcgcggccc
     1141 ggccgtcgct ggcttcgtct acgtgccgga atccgatttc cgtgagcaga ttgcgcagga
     1201 tccggcgcat cgtcgagaag tcgtcgacga ccaggaagcg ggtggcggtg gggggggtag
     1261 gtggcgtggc catcggtcgg tgtcgggcgt ggaacgcgaa gcaggtcggt gctcggcggg
     1321 gaccaagaag gcaacgggcc cgctccggtt ccacctcttg gatgtcggca tctttctgtc
     1381 ctgccttgac actgaaggtg cttttcgagc gattgcgccg acgtgcggcg cctctgatcg
     1441 ccgatcgacc tagcgcatga attggcccac cgggtgcgcg gttctcggag caccccgttc
     1501 gtgcggaata ccgcaaactt gggacacgtt gatgaccggg accgcgccat ggacctcacc
     1561 gaacccaaca ccgtcggact caacaccctg aaccgcccta ccggccccgc tacgctgggc
     1621 ccgatcaccg tgccgaccgt ggtcggagcg gtctcgatgg cctctgcgga tcgcagccac
     1681 gtgtcgcgcg agatcgcatc gtggcgccag ctgaagatgt ggctcgacgg tgaaagcctg
     1741 cagtcggccg tcaccgtcaa gttgattgga gtgcacgagg gcaagtcgct gcttgtgacc
     1801 tcccccgacg aaacgcagct ctatctgcgc gaaggacggc tgtaccggtt ccgcagcttc
     1861 tccggcgagt tcgtctacga attcgtcgcg ccgctgatca aggtctgcac ggcgccgttc
     1921 ccgtaccttc acatcggttg ggcaggcagc cgccaggtcg aaaaacgcaa cctgcgcgcc
     1981 gcgcgtcgcg tccgcacgga tctccagtgc atggtctatc cgggccagca ggccagcgga
     2041 cggttcgcca agggcctgat ccatgacctg tcgaccgccg gcgccgcgat cacgctgcag
     2101 gatgaactcg ccgtcttcta cgacgaagtc aaggtcgtgt tccgcgtgca ggtcgctgaa
     2161 caggaaatcc tcatcgaagc gcgcgcacgc gccgtgcgca aacccgaggc aggtgctccc
     2221 gatccgaccc tcggtgtcgc cttcgtcggt ctcggcgacc tggaacgact cgcgctgcac
     2281 gcctacgtca attcctcgct cgtgcaggaa ctggagctgc cgctttacgc ttgacgctcc
     2341 tcgtgtacgc gagccctttg ggctcgcacg tgcactcgtc gcggcacggt gctcgcttcg
     2401 cgagctccgc tcaagactag atagtcggct gcgctccgga gcggccgcgc gctccgccga
     2461 aggtcaaccc attgcactcc accccgcgtt gcacctcgtc gtgccagggt tgcctctccc
     2521 tcctcgccag gcgaggaggg tgcagggagg agtgccccat tccctctcgc tcggcgagag
     2581 ggcaagggtg agtgtcgt
//
LOCUS       DATIRT010000004         1908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1671873, whole genome shotgun sequence.
ACCESSION   DATIRT010000004 DATIRT010000000
VERSION     DATIRT010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1671873"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VLN25_00070"
     CDS             complement(<1..302)
                     /locus_tag="VLN25_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09002.1"
                     /translation="MRGKVSKSRELVLVPENRRDIDAVARLCRKLATRRALISAGASV
                     VPIPGIDIAVDIGVLMSMIEDINAAFGLTPDQIDRLAPRRKVFAYKAVMAVGSALVG"
     gene            603..959
                     /locus_tag="VLN25_00075"
     CDS             603..959
                     /locus_tag="VLN25_00075"
                     /inference="COORDINATES: protein motif:HMM:NF016244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HSQ09003.1"
                     /translation="MSTAALAGGPGQRSSLNWVAIDADPRFQLLHERKVKFLAGLMIF
                     SVAYYFLLPIGAAYFPELFRIKVWGPLNVGILFALSEFVVAWGIAFIYARRANREFDA
                     MARELIADAENIGRAS"
     gene            956..>1908
                     /locus_tag="VLN25_00080"
     CDS             956..>1908
                     /locus_tag="VLN25_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HSQ09004.1"
                     /translation="MSRRTNMFLVAGVLALPLIALPAFAQGAVGSEWRWLTFAVFAAI
                     IGLTMFVTFLAAKRVKSAADFYTAGGGVSGLQNGWAIAGDYLSAASFLGIAGLISLYG
                     YDGFMYSVGWLVAYITVLLVIAEPCRNIGKYTLADILAYRNDPKKVRVVGAISVITVS
                     TFYLTAQMVGGGVLVKTLIGIDYEISVIVVGILMLGYVLFGGMVATTWVQIIKAILLV
                     AASLILVMLVWTPSGFSLPAFLQDVVGDPKVQARVASLLGDPARNMSAIELGQRFLEP
                     GLLFKAPIDQISLGMALVFGTAGLPHILMRFFTVPTAQDAR"
BASE COUNT          318 a          610 c          600 g          380 t
ORIGIN      
        1 cccacgagcg cgctgccgac agccatcacg gccttgtagg caaacacctt gcggcggggc
       61 gcgagccggt cgatctggtc gggcgtcaag ccgaacgcgg cattgatgtc ctcgatcatc
      121 gacatcagca caccgatgtc caccgcaatg tcgatgcccg gaatcgggac gaccgaggca
      181 ccggcggaaa tcaaggcacg ccgcgtcgcc agcttgcggc aaagacgcgc gaccgcgtcg
      241 atgtcgcgcc ggttctccgg cacgagaacc agttctcgcg atttgctgac tttgcctctc
      301 acgctccaat gctaccggtc gtcgctcgga atacacggga gagcgtgacg acgctccagc
      361 gcaaaccccg aggaacacac tgcagacgga gggcttagcg cgtcgcagca aatcgcctcc
      421 gctagacatg gatcaagtcg ccacccatgt ccggtatgtc aacaaaccgc atcgacgcct
      481 atcgtgcggc tgccgacaag aacaagtgcg cagcgcggtc cgcatcctgc agggtcgcgt
      541 gaacgacgga tagcaacact cgaccgcggc atccgtcgcg tccatttcat cgggaggaaa
      601 acatgtccac tgctgcactg gccggcgggc cggggcaaag atcgtcgctc aactgggttg
      661 cgatcgacgc ggacccgcgc tttcagttgt tgcacgagcg caaggtcaag ttcctggccg
      721 ggctgatgat cttttccgtt gcctactact tcctgttgcc gatcggcgcg gcgtacttcc
      781 ccgaactttt ccggatcaag gtctggggtc cgttgaatgt cggcatcctg tttgcgctgt
      841 cggaattcgt ggtggcgtgg ggcatcgcct ttatctacgc acggcgcgcc aaccgtgagt
      901 tcgacgccat ggcccgtgaa ctgatcgccg acgccgaaaa catcgggagg gcgtcatgag
      961 ccgtcgcacg aacatgttcc tggtggcggg cgttctggca cttccgctga tcgcattgcc
     1021 ggcatttgcg caaggcgctg tgggttccga gtggcgctgg ctcacgttcg cggtcttcgc
     1081 agccatcatc ggcctgacga tgttcgtcac cttcctcgcc gccaagcggg tgaagtcggc
     1141 cgccgacttc tacacggctg gtgggggtgt ctccggattg caaaacggct gggcgatcgc
     1201 cggggactat ctcagtgccg cttcgttcct cggcatcgcc gggttgatct cgctgtacgg
     1261 ctatgacggg ttcatgtact cggtgggctg gctggttgcg tacatcaccg tattgctggt
     1321 gattgccgaa ccgtgccgca acatcggcaa atacacgctg gccgacatcc tggcgtatcg
     1381 caacgatccg aagaaggtgc gcgtggtcgg agcgatctcg gtcatcaccg tgtctacctt
     1441 ctacctgacg gcgcagatgg tcggcggcgg cgtgctggtc aagacgctga tcggcatcga
     1501 ctacgagatc tccgtcatcg tcgtcggcat cctgatgctg ggctacgtgc tgttcggcgg
     1561 catggtcgcc accacgtggg tgcagatcat caaggccatc ctgctggtgg ccgcgtcgct
     1621 gatcctggtg atgctggtgt ggacgccctc cggcttctcg ctgccggcgt tcctgcagga
     1681 cgtggtcggc gatcccaagg tgcaggcgcg cgtcgcgtcg ttgctcggtg acccagcccg
     1741 caacatgtcg gcgatcgagc tcggccagcg cttcctcgag ccgggcctgc tgttcaaggc
     1801 gcccatcgac cagatctcgc tcggcatggc gctcgtgttc ggcactgcgg ggctgccgca
     1861 catcctcatg cggttcttca ccgtgcccac cgcccaggac gcgcgcaa
//
LOCUS       DATIRT010000005        12165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1573708, whole genome shotgun sequence.
ACCESSION   DATIRT010000005 DATIRT010000000
VERSION     DATIRT010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12165
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1573708"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1344)
                     /gene="guaB"
                     /locus_tag="VLN25_00085"
     CDS             complement(<1..1344)
                     /gene="guaB"
                     /locus_tag="VLN25_00085"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HSQ09005.1"
                     /translation="MRLVQKALTFDDVLLVPAHSAVLPRDVQLSTKLSRKIHLNIPLV
                     AAAMDTVTEARLAIALAQEGGIGIIHKNFSPKEQAAEVLKVKRHEAGVLRDPITLTQD
                     MTVRDAIELQRSHKISGLPVVEGKKIVGIVTNRDLRFETRIDAPIRDIMTPRSRLVTV
                     REGASLDEVKALMHTHRLERVLVVNDADDLLGLVTVKDITKATEHPNAAKDEQGKLRV
                     GGAISTGTDSEERAELMVKAGVDVLIVDTAHGHHQGVIDRVRWVKKNFPQVDVVGGNI
                     ATASAAKALADAGADGIKVGIGPGSICTTRIVAGVGVPQITAVANVAKALEGTGIPLI
                     ADGGIRFSGDVSKALAAGAYAVMMGGMFAGTEEAPGEVILYQGRSYKGYRGMGSLGAM
                     TEGSAERYFQDNDANVDKFVPEGIEGMVPYKGSVLAIIYQLTGGLRSSMGYCGCST"
     gene            1516..2058
                     /locus_tag="VLN25_00090"
     CDS             1516..2058
                     /locus_tag="VLN25_00090"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HSQ09006.1"
                     /translation="MVHSVPSWRLAVLVSLGLLAWSLAAPAAAQWAWKDDNGRVVYSD
                     RPPPSAVKGDRIVRQPSNAQTVLPPQQGATDPTAKPATDAKPVAASSGPKSVAEQEME
                     FRKRQQERADAEKKTQDDQVKVAAKAAECERARGYLKGIEEGQRIVRSDASGNREYLD
                     DSQRAAEAERTRKIVQSSCS"
     gene            complement(2083..2379)
                     /locus_tag="VLN25_00095"
     CDS             complement(2083..2379)
                     /locus_tag="VLN25_00095"
                     /inference="COORDINATES: protein motif:HMM:NF015611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HSQ09007.1"
                     /translation="MIVTVVYAGSKRRIVLRVDVDDGVTIEQAIIASGVLSLEPGLHL
                     KDLSVGVWNRAARLEQRVCEGDRIEIYRPLQVDPKEARRLRADIRRRRPPDGGA"
     gene            complement(2376..2816)
                     /locus_tag="VLN25_00100"
     CDS             complement(2376..2816)
                     /locus_tag="VLN25_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HSQ09008.1"
                     /translation="MPHVERSVLVHYSAEQMFDLVADVEGYPGFLPWCSGAQVAADHG
                     GGMDASLDIDYRGIRARFTTRNQLRYPEQIRMSLVNGPFRSLDGHWQFHALRPGACKV
                     QLKLHYEFASGLLGRAVAPVFDFIANSMIDSFARRAEFIYGPSE"
     gene            2869..3318
                     /gene="smpB"
                     /locus_tag="VLN25_00105"
     CDS             2869..3318
                     /gene="smpB"
                     /locus_tag="VLN25_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HSQ09009.1"
                     /translation="MAIVQNKKAQHDYFIEDRYEAGMVLEGWEVKAIRAGRAQLNEAY
                     VVIRSGELFLLNGHISPLPTASTHINPDPVRTRKLLLNAEEIRKLIGKVERAGYALVP
                     LDLHYSKGRVKLEVGLAKGKRQFEKRSSEADKDWKRQQTRLLKQRSR"
     gene            complement(3372..3992)
                     /locus_tag="VLN25_00110"
     CDS             complement(3372..3992)
                     /locus_tag="VLN25_00110"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HSQ09010.1"
                     /translation="MGILEWLSGRSRKSGSERWVVVDVETSGMNVASDELIAIGAVAM
                     HDGRVVPGDSIEIIVKQSAVSSRENILVHGVGAQAQLNGTDPAHAVRAFMDYVGRAPM
                     LAFHAPFDRGFLARVTKHYVNQPFDNPWLDLAELAPALEPKVRLRSLDEWLQHYRIPV
                     SVRHSAAADAFATALLMARLLPEARRQGSVGFNAMQQLARQAKWLH"
     gene            complement(3994..5892)
                     /locus_tag="VLN25_00115"
     CDS             complement(3994..5892)
                     /locus_tag="VLN25_00115"
                     /inference="COORDINATES: protein motif:HMM:NF021824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HSQ09011.1"
                     /translation="MAAPTIAAGLRQLLSAHLPFSAMAPADLDLIIDNVQIAYYAPGE
                     IVLAPSGAVPTHCLIVKQGRVQGEAPDKKGIAYEAGVGDCFPVGALLAERAVTLTYRS
                     VGDTFCLLLPREKFFELAQRSGPFMDICKRRLGALLDLSRQQLQQNYATEASAERTMG
                     TPLGELLRAQPLTCSPDTPLRDAFGRMHANHVGSIVVVEQSPVGEIVSGILTRTDLIG
                     RVILPEVPLSTPVSTVMTRKVLTLDADETAADATLLMAEHSIRHIPVVRKVDGKTRVA
                     GVVSERDLFALQRLSVRQLAVAIRRSEDVDALVVAAADVRRLSHHLVAQGVAAAQLTR
                     LISHLNDQMTVRLLTLGTQRYSVDPQSFCWLSFGSEGRGEQTIATDQDNGILYVAEAT
                     TQARMLELANWTNEQLAACGFPLCKGNIMARNPAWCLDYAGWDALFSGWIDRGDPEAL
                     LNASIFFDFRPLYGDAGLANGLREEVVQRAERNGRFLKQMADNALRNRPPAGRGIVDS
                     LFGEGGPARVDLKMHGTVPFVDAARIWALAAGLHETNTAERLKRLGESGRLPEVDVRA
                     WVPSFEFFQLMRLRAQHRRAQTYAGTNDNPNEIELAELSALDRRIVNEAFRQARKVQQ
                     RLELDYPG"
     gene            complement(6067..7008)
                     /locus_tag="VLN25_00120"
     CDS             complement(6067..7008)
                     /locus_tag="VLN25_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HSQ09012.1"
                     /translation="MPIPYVDGVSIIAVVILVVAVTFVIKAVKVVPQQNAWVVERLGK
                     FHAVLQPGLNIVIPFVDRLAYKHSLKEVPLDTPSQVCITKDNTQLQVDGVLYFQVTDP
                     QRASYGASNYILAITQLAQTTLRSVIGKLELDKTFEEREFINHSVVSAIDEAAMNWGV
                     KVLRYEIKDITPPNAILHAMQAQITAEREKRALIASSEGRKQEQINIATGAREAAIAK
                     SEGDKQAQINVALGEASAIVAIADATAKAIRQVAQATREPGGLEAVNLKVAERYVDAF
                     SNLAKQNNTLIVPANLGDVAGLISTAMTVIKAQPAAQ"
     gene            complement(7018..7446)
                     /locus_tag="VLN25_00125"
     CDS             complement(7018..7446)
                     /locus_tag="VLN25_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HSQ09013.1"
                     /translation="MDSYIVWFVVAFGLLVAELLTGTFYLLVISIALAVAGVSAWLGA
                     PTALQFVVAAAIGLGGSMWLRATRFGKRLHERGDDRVQNMDIGQSLRVENWTAARTAR
                     ANYRGAVWDVELAPGEPAASGEFVIREINANRLIVAPKPR"
     gene            complement(7543..9936)
                     /gene="ppsA"
                     /locus_tag="VLN25_00130"
     CDS             complement(7543..9936)
                     /gene="ppsA"
                     /locus_tag="VLN25_00130"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HSQ09014.1"
                     /translation="MPRGEYVLPFSQLRMTDVEAVGGKNASLGEMLSQLSDAGIRVPD
                     GFATTADAFRAFIDGGGLRQRIADALDRLDVDDVRTLAATGAEIRSWLTATPFPPQLE
                     DQIRSSFQLFEKSSSSEMSFAIRSSATAEDLPDASFAGQQETFLNVVGIEAVLDRIKH
                     VFASLYNDRAIAYRVHKGFAHADVALSAGIQRMVRSDRGAAGVMFTMDTESGFRDVVF
                     ITSSFGLGETVVQGAVNPDEFYVHKPMLAAGKHSILRKSLGSKLIKMEFDPAGGAGRT
                     VRTVDVSDAERHRFSITDADVLELARYAVQIEQHYGRPMDIEWGKDGADGRIYILQAR
                     PETVQSQVSVETQQRFKLKGKGAVLISGRAIGQKIGVGVVRNIRSSTEMERVLPGDVL
                     VTDMTDPNWEPVMKRAAAIVTNRGGRTCHAAIIARELGIPAVVGCGHATEVLKEGATV
                     TVSCAEGDTGNIYAGALEMEIETLQQGALPKIPVKIMMNVGNPTLAFDFQALPNAGVG
                     LARLEFIINNNIGVHPKAVLDYPNLDHDLKVAVERLAAGYDSPRAFFEAKLAEGIATI
                     GAAFWPKPVIVRMSDFKSNEYKKLIGGSRYEPDEENPMLGFRGAARYIADSFRECFRM
                     EAAALKRVREVMGLTNVEIMVPFVRTLGEAKRVTELLAESGLQRGSGAVDGAGGLRLI
                     MMCEVPSNALLANQFLDYFDGFSIGSNDLTQLTLGLDRDSGLVAEGFDERDPAVKALL
                     QMAISACRARGKYIGICGQGPSDHADFAEWLMEQGIESISLNPDTVVATWQRLARAA"
     gene            10026..10874
                     /locus_tag="VLN25_00135"
     CDS             10026..10874
                     /locus_tag="VLN25_00135"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HSQ09015.1"
                     /translation="MPDRHLGPFDRTVFFVSDGTGITAETLGHSVMTQFEDLRYRPQR
                     IPFIDSVEKAREAAARINDAGGRDGKRAIVFSTLVNPPVAAALRAEAQGFFIEMFETF
                     VEPLERELGMKSAHTIGRSHNIATNSEDYKNRIEAINFALAHDDGQSAKGLEKADVIL
                     VGVSRSGKTPTTLYLAMQYGVKAANYPLIPEDFERNQLPSALPPYKGKIFGLSISPER
                     LSEVRNERRPNSRYASVENCRYEVDAAERMMRREGIRWLSSTTKSIEEIAATIMQEVR
                     LDRRSY"
     gene            complement(10925..11704)
                     /locus_tag="VLN25_00140"
     CDS             complement(10925..11704)
                     /locus_tag="VLN25_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HSQ09016.1"
                     /translation="MQITSADNPLVRRLRRLAESPRASRTAGRSIAEGLHLVECAVAA
                     GVAIETIVVSDRANEAARELALQLTAQGACRHVSLAESLYDAISPVEHGAGILAEIVI
                     EPSALPVAMVADAVYLDGVQDPGNVGTLLRTAAAAGVKHVAAAPGTAFLWAPKVLRAA
                     MGAHFALKLYNDVGPGHLSAAFVGERLSADAHAADSIYSTAWGSQPTVWLFGSEGQGL
                     SAAAEAVTQRRLRIPVDASVESLNVAAAAAVCLFKQRRRRA"
     gene            complement(11682..>12165)
                     /locus_tag="VLN25_00145"
     CDS             complement(11682..>12165)
                     /locus_tag="VLN25_00145"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HSQ09017.1"
                     /translation="LSPARRGELALLIREHSMAWAVAEASVGEIDALNILQATLLAMR
                     RAVELLALPAEYALIDGNQMPRLAIPGHAIVAGDATQPSISAASILAKTARDALMCAL
                     DAQHPGYGFSRHMGYATAEHLHNLQRLGPCEVHRKSFAPVRELLAGIPDTQHADHVGR
                     "
BASE COUNT         2180 a         4046 c         3776 g         2163 t
ORIGIN      
        1 cgtgctgcaa ccgcagtagc ccatgctcga tcgcagtcct ccggtcagct ggtaaatgat
       61 cgccagcacg ctgcctttgt agggcaccat gccttcgatg ccttcgggga cgaacttgtc
      121 cacgttggcg tcgttgtcct ggaagtagcg ttcggccgag ccttcggtca ttgcccccag
      181 gctgcccatg ccgcggtagc ccttgtagga acgcccctgg taaagaatga cctcgcccgg
      241 cgcctcttcg gtgccggcaa acatgccgcc catcatgacg gcgtaggccc cggccgcaag
      301 agccttcgaa acgtcgcccg agaaacggat gccaccgtcg gcgatcaacg ggattccagt
      361 gccctcgagc gccttggcga cgttggccac cgcggtgatc tgcgggacgc cgacgcctgc
      421 gacgatgcgc gtggtgcaga tcgaacccgg accgatgccg accttgatgc cgtcggcgcc
      481 ggcgtcggcc agcgccttcg cagcggacgc agtggcaatg tttccgccga caacgtccac
      541 ctgcggaaag ttcttcttaa cccagcggac ccggtcgatc acgccctggt ggtgaccgtg
      601 ggccgtgtcg acgatcagca cgtcgacacc cgccttgacc atcaattcgg cgcgctcctc
      661 ggaatcggtc ccggtgctga tcgcgccgcc gaccctgagc ttgccttgtt cgtctttcgc
      721 agcattcgga tgctcggtgg ccttcgtgat gtccttgacg gtgaccagtc caagcagatc
      781 gtcggcgtcg ttcacgacca gcactcgttc aagccggtgc gtgtgcatca gcgcctttac
      841 ctcgtccagg ctcgcaccct cgcgcaccgt caccaggcgc gagcggggcg tcatgatgtc
      901 ccggatcggc gcgtcaattc gcgtctcgaa gcgcaggtcg cgattagtga cgatgccgac
      961 gatcttcttg ccttcgacga ccggcaggcc tgaaatcttg tgtgaacgct gcagttcgat
     1021 cgcatcgcgc accgtcatgt cctgcgtgag cgtgatcgga tcgcgcagca cgccggcttc
     1081 gtggcgcttg accttcagca cctcggccgc ctgctctttc ggcgaaaagt tcttgtgaat
     1141 gatcccgatt ccgccttcct gcgccagtgc gatcgcgagg cgcgcctcgg tcacggtgtc
     1201 catggcggcc gcgaccagcg gaatgttcaa atggatcttg cgggagagct ttgtggagag
     1261 ctggacgtcg cggggaagga ccgccgagtg ggcggggacg agcagcacgt catcgaaggt
     1321 cagcgccttc tgaaccaacc gcataggtgt cctcatgacg caaaagcgga gtataccgac
     1381 tcgatttctg gtcgactttg cccggtttcc ttcccgactc ggcggtttaa cctaaaagtg
     1441 ggggtgagtt cacccgccat tcgacttgcc tggtcgccgc gaagcgatgg agaattcctg
     1501 cgctgaggag gatctatggt gcattcggtg ccgtcatggc ggttggccgt actggtttcg
     1561 ctgggtttgc tcgcctggag cctggccgcg ccggctgcgg ctcagtgggc ctggaaggac
     1621 gacaacggtc gtgtggtcta ttcggaccgc ccccctcctt cggccgtgaa gggcgaccgc
     1681 atcgtgcgtc agcccagcaa tgcccagacc gtgctgccac cccagcaggg cgcgaccgat
     1741 ccgacggcca aacccgccac cgacgccaag ccggtcgccg cgtccagcgg tccgaaatcg
     1801 gtcgcggaac aggaaatgga attccgcaaa cggcagcagg aacgggccga tgcggaaaag
     1861 aaaacgcagg acgatcaggt aaaagtagcc gccaaggcag cggagtgtga gcgtgcccgc
     1921 gggtacctga aagggatcga ggaaggccag cgcatcgttc gcagtgatgc atcaggcaat
     1981 cgtgagtatc tcgacgacag ccagcgtgcc gccgaggccg aacgcactcg caagatcgtc
     2041 cagtcgagct gcagttagtc ccgagtccct agtccctagt ctctaggccc cgccgtccgg
     2101 cgggcgtcgg cgacggatgt cggcacgaag acgccgcgct tccttcggat cgacctgcag
     2161 cggtcggtag atctcgatgc gatcaccttc acacacgcgc tgttccagtc tggccgcgcg
     2221 attccacacg ccgacactta aatccttcaa atgcagcccg ggttcgagcg acagcacgcc
     2281 cgaggcgatg atggcttgct cgatcgtcac tccatcgtcg acatcgaccc gcagcacgat
     2341 ccgccgtttc gacccggcgt agaccaccgt gacgatcact cgctcggccc gtagatgaac
     2401 tcggcgcgcc gggcaaacga gtcgatcatc gagttcgcga tgaagtcgaa aacgggagcg
     2461 accgcgcgcc ccagcaagcc actggcgaac tcgtagtgca gcttcagctg caccttgcac
     2521 gcgccgggcc gcagcgcatg gaactgccag tgaccatcga gcgaccggaa cgggccattg
     2581 accagcgaca tgcggatctg ctccgggtag cgcaactggt tgcgcgtcgt gaacctcgcg
     2641 cgaattccgc ggtagtcgat gtccaggctt gcatccatgc cgccgccatg gtctgcggca
     2701 acctgagctc cgctacacca gggcaggaag cccggatagc cctccacgtc ggccaccaaa
     2761 tcgaacatct gctcggcgct gtaatggacc aggacggatc gttcgacgtg cggcatggcg
     2821 agtgggatat aaacgcggat tctattcacg tcgggcaggc ggtttgccgt ggcgatcgtc
     2881 cagaacaaga aggcgcagca cgattacttc atcgaggacc ggtacgaagc cggcatggtc
     2941 ctcgagggct gggaagtcaa ggccatccgc gccggtcgcg cgcagctcaa cgaggcctac
     3001 gtcgtgatcc gctcgggcga gctcttcctg ctgaacggtc acatcagccc gttgccgacc
     3061 gcgtccaccc acatcaaccc cgatccggtc cgcacgcgca agctgttgct gaacgccgaa
     3121 gagatccgca agctcatcgg caaggttgaa cgcgcgggct acgcgctggt tccgctcgac
     3181 cttcactaca gcaagggacg agtcaagctt gaagtcggac tcgccaaggg caagcggcag
     3241 ttcgagaaac gctcaagcga agccgacaag gactggaagc ggcagcagac gcggctgcta
     3301 aagcagcgca gccgatagtc ttccgtggac ttgccctgcc tgcggcacga ccggagtctg
     3361 cgggatccgc ctcaatgcaa ccacttagcc tgtcgggcca gctgctgcat cgcgttgaaa
     3421 ccaaccgacc cctgccgacg ggcctcgggc agcagacgcg ccatcagcaa ggcggtcgcg
     3481 aaggcatcgg ccgccgcact gtgccggacg gacaccggaa tcctgtagtg ctgcagccat
     3541 tcgtccagcg accgcagccg gaccttcggc tccagcgccg gtgcaagttc ggccaggtcc
     3601 agccacggat tgtcgaacgg ctgattcacg taatgcttgg tgacccgggc caggaacccg
     3661 cggtcaaagg gcgcatggaa cgccagcatc ggggcgcgac caacatagtc catgaacgcc
     3721 ctcactgcat gcgccgggtc cgttccgttg agctgtgcct gcgccccgac accatgcacg
     3781 aggatgttct cgcgcgaact caccgcactc tgcttgacga tgatttcgat gctgtctccc
     3841 ggaacgacgc gtccatcgtg cattgcgacc gcgccgatgg cgatgagctc gtcactcgcg
     3901 acattcatgc cgctggtctc gacatcgacg acgacccagc gctcggaacc cgacttgcgg
     3961 cttcggcccg agagccactc aaggatcccc atgtcagccc ggatagtcca actcgagacg
     4021 ctgctgcact ttgcgcgcct gacggaaggc ttcgttgacg atgcgacggt ccagcgctga
     4081 aagttcggcc agctcgatct cgttcggatt gtcgttggtg ccggcatagg tctgagcacg
     4141 gcgatgctgc gcgcgcagac gcatcagctg gaagaactcg aacgagggga cccacgcacg
     4201 cacatcaact tccggcaggc gtcccgactc tccgagccgc ttcaggcgtt cggccgtgtt
     4261 ggtttcgtgc aggccagctg cgagtgccca gattcgcgcg gcgtcgacaa aaggtacggt
     4321 gccgtgcatc ttcaggtcga cgcgcgccgg cccgccctcg ccgaacagcg aatcgacaat
     4381 gccacggcct gcgggcgggc gattgcgcag agcgttgtct gccatctgct tgaggaagcg
     4441 cccgttgcgc tcggcacgct gcacgacttc ttcgcgcagt ccgttggcga ggccggcgtc
     4501 accgtacagt ggacggaagt cgaagaagat gctggcgttg agcagtgctt ccggatcgcc
     4561 gcggtcgatc cagcccgaga acagcgcatc ccagccggcg tagtcgagac accacgccgg
     4621 gttgcgcgcc atgatgttgc ccttgcacag cggaaatccg cacgcggcca gctgctcgtt
     4681 ggtccagttg gccagttcga gcatgcgcgc ctgggtcgtc gcctccgcca cgtacaggat
     4741 gccgttgtcc tggtcggtgg caatggtctg ctccccccgc ccctcgctcc cgaatgacag
     4801 ccagcagaag ctctgcggat cgaccgagta gcgctgtgtg ccgagcgtca gcaggcgcac
     4861 ggtcatctgg tcgtttagat ggctgatcag ccgggtcagc tgcgccgcgg cgacaccctg
     4921 cgcgaccaga tggtgtgaca ggcgacgcac gtcggccgcc gccacgacca gggcatcgac
     4981 gtcctcactg cgtcggatcg ccaccgcgag ctgccgaacg ctcagccgtt gcagcgcaaa
     5041 caggtcacgc tccgagacca cgcccgccac gcgcgtcttg ccgtccactt ttcgtaccac
     5101 cggaatgtgg cggatcgaat gctctgccat cagcagcgtc gcatcggcgg ccgtctcgtc
     5161 cgcgtccagc gtcagcacct tacgcgtcat caccgtgctc accggtgtcg acaaaggcac
     5221 ctcaggaagg atgacgcgtc cgatcaggtc cgtgcgcgta aggattccgc tgacgatctc
     5281 gcccaccggg ctttgctcga cgaccacgat cgatccgaca tgattcgcat gcatccgccc
     5341 gaaggcgtcg cgcaacggcg tgtcgggcga acaggtcagt ggctgcgccc tcagcagttc
     5401 cccgagcgga gtacccatcg tccgctcggc agatgcctcg gttgcgtagt tctgctgcaa
     5461 ctgctgccgc gacaggtcga gcaaagcccc gaggcgacgc ttgcagatgt ccatgaacgg
     5521 gccgctgcgc tgcgcgagct cgaaaaactt ctcgcgtggc agcagcaggc agaacgtgtc
     5581 gccaaccgag cggtaggtca gcgtcaccgc gcgttccgca agcaaagctc cgaccggaaa
     5641 acaatcgccg acgccggcct cgtaggcaat gcctttttta tcgggcgcct cgccctggac
     5701 acggccctgc ttgacgatca ggcagtgggt cggaacggcg ccgctcggcg cgagtacgat
     5761 ttcaccgggg gcgtagtagg cgatctggac gttgtcgatg atcaggtcca ggtccgccgg
     5821 cgccatcgca gagaacggca gatgggcgga taaaagctgc cgcagtcctg ctgcgatcgt
     5881 gggtgctgcc atgccgtctc ctcagggctc gcgggcagcc gatcgccgac tcacccgtcc
     5941 cgttccttgt cctgttgtcc ggtcgagcgg tgccgaccgc gcgcgccgag gatcatccac
     6001 acggccagcg ccaggccggc acctgcgatg acgaactcca cgtgatcttc ctatcgtccc
     6061 atcgtcctat tgcgcggccg gctgcgcctt gatgacggtc atggccgttg aaatcaggcc
     6121 cgccacgtcg ccgaggttgg ccgggacgat cagcgtgttg ttctgcttcg ccaggttgct
     6181 gaacgcgtcg acgtaacgct ccgcgacctt gaggttgact gcctccagtc cccccggttc
     6241 gcgcgtggcc tgtgccacct gccggatggc ctttgcggtc gcatcggcaa tcgcaacgat
     6301 ggccgaggcc tctccgagcg ctacgttgat ctgcgcctgc ttgtcgcctt cggacttggc
     6361 gatcgcggct tcacgcgcgc cggtggcgat attgatctgc tcctgcttgc gaccctcgga
     6421 cgacgcgatc agcgcgcgct tctcgcgttc cgcagtgatc tgcgcctgca tggcatgaag
     6481 aatcgcattc ggtggcgtga tgtccttgat ctcatatcgc agcaccttca caccccagtt
     6541 catcgccgcc tcgtcgattg ccgacacgac gctgtggttg atgaactccc gttcctcgaa
     6601 ggtcttgtcg agctcgagct tgccgatgac cgagcgcagg gtcgtctgcg ccagctgcgt
     6661 gatcgcgagg atgtaattgc tggcgccgta ggacgcccgc tgcggatcgg tgacctggaa
     6721 gtacagcacg ccgtcgacct gcaactgggt gttgtccttg gtgatgcaga cctggctcgg
     6781 cgtgtcgagc ggaacctcct tcaggctgtg cttgtaggcg agccgatcga cgaaaggaat
     6841 cacgatgttc agtcccggtt gcagcaccgc gtggaacttc ccgagccgct cgaccaccca
     6901 ggcattctgc tgcgggacga ccttgactgc cttgatgacg aaggtgacgg ccaccacgag
     6961 gatgacgacc gcgatgatcg ataccccgtc aacgtatgga atcggcatga ctgtctccta
     7021 gcgcggtttg ggtgcaacga tcaggcggtt ggcgttgatc tcgcggatca cgaactcgcc
     7081 gctggcggcc ggttcaccgg gcgccagctc gacatcccag accgcgccgc ggtagttcgc
     7141 gcgagcggtg cgggcagcgg tccagttctc gacgcgcagc gactggccga tatccatgtt
     7201 ctggacccga tcgtcgcccc gctcgtgcag ccgcttgccg aagcgcgtcg cacgcagcca
     7261 catcgaaccg ccgaggccga tcgcggccgc cacgacgaac tgcagcgccg tcggtgcacc
     7321 gagccacgcc gacactccgg caacggcgag cgcaattgag atcaccagca ggtaaaacgt
     7381 gccggtcagc agctcggcga ccagcagccc gaaggcgacg acgaaccaca cgatgtaact
     7441 gtccatcaca gtcttcctct tgcgctaggt gcggatcggg cgtggcaatt caagcaggcc
     7501 ggcgtcgacg ccgacgaaaa ggcgccaccg atgcgtcccg gttcatgccg cgcgcgccag
     7561 ccgctgccac gtcgccacca ccgtgtcggg attgagcgag atcgactcga tgccctgctc
     7621 catcagccat tcggcgaagt cggcatggtc cgacggaccc tgtccgcaga tgccaatgta
     7681 cttgccgcga gcgcggcacg cactgatcgc catctgcaac agtgccttga ccgccggatc
     7741 gcgctcgtcg aagccttcgg cgaccagccc ggagtcgcgg tccagcccca gcgtcaactg
     7801 ggtcaggtcg ttcgatccga tcgagaaacc atcgaagtaa tcgaggaact ggtttgccag
     7861 caatgcgttc gacggcactt cgcacatcat gatcaaccgc agcccgccgg ctccgtcgac
     7921 cgcgccgctg ccgcgctgca ggccgctttc cgccagcagc tcggtcacgc gcttcgcctc
     7981 ccccagcgtg cgcacgaacg ggaccatgat ctcgacattg gtcaggccca tgacctcgcg
     8041 cacgcgcttg agcgccgccg cttccatgcg gaagcattcg cggaacgaat cagcgatgta
     8101 gcgcgccgcg ccgcgaaacc ccagcatcgg attttcttcg tccggttcat accgcgagcc
     8161 accaatcagc ttcttgtact cgttgctttt gaagtccgac atccgcacga tcaccggctt
     8221 cggccagaag gccgcgccaa tcgtcgcgat gccctcggcg agcttggcct cgaaaaaagc
     8281 gcgcggcgag tcgtagccgg cggcgagtcg ctcgaccgcc actttcaggt cgtgatcgag
     8341 gttcggatag tcgagcaccg ccttcgggtg cacgccgatg ttgttgttga tgatgaactc
     8401 gagccgggcc agaccaacgc cggcattcgg gagggcctgg aaatcgaaag ccagcgtcgg
     8461 gttgccgacg ttcatcatga tcttgaccgg gatcttcggc agtgccccct gctgcagcgt
     8521 ctcgatttcc atttcgagcg ctccggcata gatgttgccg gtatcccctt cagcacagga
     8581 caccgtcacc gtcgcgcctt ccttcagcac ttcggtcgcg tggccgcagc ccacgacggc
     8641 cgggatgccc agctctcgcg caatgatggc ggcatggcag gtgcggccgc cgcggttggt
     8701 gactatcgcc gctgctcgct tcatcaccgg ctcccagttg ggatcggtca tgtcggtcac
     8761 caggacatcg cccggcagca ctcgctccat ttcggtggag ctgcggatgt tgcgcacgac
     8821 cccgacaccg atcttctggc cgatcgcgcg cccggagatc aggaccgctc ccttgccttt
     8881 cagcttgaag cgctgctgcg tttcgacgct cacctgagac tgcactgtct cgggacgcgc
     8941 ctgaaggatg tagatgcgtc cgtcggcacc gtccttgccc cactcgatgt ccatcggacg
     9001 gccgtagtgc tgttcgatct gcactgcata acgcgccagt tcgagcacat cggcgtccgt
     9061 gatcgagaag cggtggcgct cagcgtcgga tacgtcgacc gtgcgcacgg tccgaccggc
     9121 gccgccggcc ggatcgaact ccattttgat cagtttggaa cccagggact tgcgcaggat
     9181 ggagtgtttg ccggccgcga gcatcggttt gtggacgtag aactcgtccg ggttgaccgc
     9241 cccctggacc accgtttcgc ccagcccgaa gctcgacgtg atgaagacga cgtcgcggaa
     9301 accggactcc gtatccatcg tgaacatcac gccggcggcg ccgcgatccg atcggaccat
     9361 gcgctggatg ccggccgata gcgcgacgtc ggcgtgggcg aaccccttgt ggacgcggta
     9421 ggcgatcgcc cggtcgttgt agagcgaggc gaagacgtgc ttgatccggt ccagcactgc
     9481 ttcgatgccg acgacgttga ggaacgtttc ctgctgcccg gcaaaagaag cgtccggcag
     9541 gtcttcggcg gtcgcactgg accgaatcgc aaatgacatt tcacttgaag aagacttctc
     9601 aaataattga aaagatgaac gaatctgatc ttcaagctgg ggcggaaacg gggtggcagt
     9661 cagccaggac cgtatctcgg ctccagtcgc ggctaacgtg cggacgtcgt cgacgtcgag
     9721 tcggtcgagc gcatcggcga tccgctgtcg caggccgccg ccatcgatga aggcacgaaa
     9781 cgcgtccgcg gtcgttgcaa agccatcggg cacccggatg ccggcgtccg acagctgact
     9841 caacatttcg cccagcgaag cattctttcc accgacggcc tcgacatccg tcatcctcag
     9901 ttgactgaac ggtaggacgt actcgccgcg tggcatgccc tctcctgctg tggttgtatg
     9961 actagaatct gctgattcta ccgagcggca tcgcatcgat actgcgccgc accagcgagg
    10021 acctgatgcc tgaccgccac cttggcccgt tcgatcggac ggtgttcttc gtttccgacg
    10081 gcaccggcat cacggctgag accctgggcc acagcgtgat gacccagttc gaggacctgc
    10141 gttaccggcc gcagcgtatc ccgttcatcg attccgtcga aaaggcccgc gaggcggccg
    10201 cacgcatcaa tgacgccggt ggtcgtgacg gcaagagggc catcgtcttt tccacgctgg
    10261 tcaatccgcc ggtggccgcc gcgctgcgcg cggaagcgca gggtttcttc atcgagatgt
    10321 tcgagacctt cgtcgagccg ctcgagcgcg aactcgggat gaaatccgcg cacacgatcg
    10381 gccgttcgca caacatcgcg accaactccg aggactacaa gaaccgcatc gaggcgatca
    10441 acttcgcgct ggcgcatgac gatggccagt cggccaaggg acttgagaag gccgacgtca
    10501 ttctcgtggg cgtctcgcgg tccggcaaaa ctccgaccac gctctacctt gccatgcagt
    10561 acggcgtgaa ggcggccaat tacccgctga tccccgagga tttcgaacgc aaccagctgc
    10621 catcggccct gccaccctac aagggcaaga tcttcggcct gtcgatttcg ccggagcggc
    10681 tgtccgaggt gcgcaacgaa cgtcgcccca acagccgcta tgcctcggtc gagaactgtc
    10741 gctacgaagt cgacgcggcc gagcggatga tgcgacgcga aggcatccgc tggctctcgt
    10801 ccaccaccaa gtcgatcgag gagattgccg cgaccatcat gcaggaggtc cggctcgacc
    10861 gccgctcata ctgaagcgac cccggatcca cggggatggt caaagggagc ccgcgagcgt
    10921 tgcctcatgc gcgccgtcga cgctgcttaa aaagacagac cgctgccgcc gccgccacat
    10981 tgagcgactc gacgctcgca tcgaccggaa tacgcagccg tcgctgcgta acggcctcgg
    11041 ccgcagcgct taggccctgc ccttccgatc cgaacagcca gaccgtgggt tgacttcccc
    11101 aagcggtcga gtagatcgag tcggccgcgt gcgcatcggc cgacagccgc tcgccgacaa
    11161 aggctgcgga caaatgccca ggcccaacat cgttgtatag cttcagtgca aaatgcgcgc
    11221 ccatcgcggc ccgcagcacc ttcggggccc acaggaacgc cgttcctgga gccgcggcta
    11281 cgtgtttcac acccgccgcc gcggccgtgc gcagaagggt tccgacgttg cccggatcct
    11341 gcaccccgtc cagatacacg gcgtcggcga ccatcgcgac gggcagcgcc gacggctcga
    11401 tcacgatttc ggccaggatg cccgcaccgt gctcgaccgg agaaatggcg tcgtacaggg
    11461 actccgcgag gctcacgtgt cgacatgcac cctgcgcggt cagttggagg gccagttcac
    11521 gcgcggcctc atttgcgcgg tccgatacga cgatcgtctc gatggcgacg cccgctgcaa
    11581 cggcgcactc gactagatgc aggccttcgg caattgagcg acccgccgtc cgactcgcgc
    11641 gcggcgactc tgcgagccgg cgcagtcgcc gcaccaacgg gttatcggcc gacgtgatct
    11701 gcatgctgcg tgtcagggat gccggcaagc agttcgcgta cgggcgcaaa ggacttccgg
    11761 tgtacctcgc agggtccgag gcgctgcagg ttgtgcagat gctcggccgt ggcgtagccc
    11821 atgtgccggg aaaagccata gccggggtgc tgtgcgtcga gcgcgcacat cagcgcatcg
    11881 cgcgcggtct tggccagtat cgaagccgcg gaaatgcttg gctgcgttgc gtcgccggcg
    11941 acaatggcat ggcccggaat ggccagccgc ggcatctggt tgccgtcaat gagcgcgtac
    12001 tcggccggca acgcgagtaa ctcgacagcg cggcgcatcg ccagcagcgt cgcttgcagg
    12061 atattgagcg cgtcgatctc gccaacactc gcctcggcga cggcccaggc catggaatgc
    12121 tcccggatca gcagcgcaag ctccccacgc cgcgcgggac tcagc
//
LOCUS       DATIRT010000006         3028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_98856, whole genome shotgun sequence.
ACCESSION   DATIRT010000006 DATIRT010000000
VERSION     DATIRT010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98856"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VLN25_00150"
     CDS             complement(<1..255)
                     /locus_tag="VLN25_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase N-terminal
                     domain-containing protein"
                     /protein_id="HSQ09018.1"
                     /translation="MPIYKAPLRDQQFVLHEVLGAVGEFNEVPRYADLDVQTVNHVVE
                     EAGKFCEEWLLPTNMDGDIEGCTFYPTSKAVKTPKGFKEAY"
     gene            complement(258..1049)
                     /locus_tag="VLN25_00155"
     CDS             complement(258..1049)
                     /locus_tag="VLN25_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSQ09019.1"
                     /translation="MPIATALSSIATALRPRKGDQTRAAIVDAALETASREGLEGLTI
                     GSLADQMHMSKSGVFAHFGSREDLQLAVLKAYVSRFIDEVLRPAVKKPRGLPRLEAIL
                     DRWVAFLARELTRGCIMIAGAVEYDDRPGPQRDAMVAIITGWKAELLKTIRQAVTEGH
                     LSATVDAPQMVFEIYGLMLAMHQDARLLRSADSAKRARAGLARLLASAAKATRSEPKT
                     RAGMRKRAAPPKPGRPASRSQRTSRTRQKSVTARPARSRPRAAKE"
     gene            complement(1157..2083)
                     /locus_tag="VLN25_00160"
     CDS             complement(1157..2083)
                     /locus_tag="VLN25_00160"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HSQ09020.1"
                     /translation="MDRWHGMEAFVRVVESGSFVAAAERLGLSTSSLSRLVGELEHHL
                     GARLLNRTTRRLSLTEIGQSYYERCVTLLADLAEAEDIAGQASAQPRGTVRLTCSHSM
                     EEQRVAPAIASFVERHAEVRFELVVSDRMVDLVEEGFDLAIRVGQMGSDRLVARRLGA
                     MQLLLCAAPAYLERHGVPRAPGELAKHNALTYAYSSSPRLWRFTDTGGRAHEVRVTGS
                     LHANSGDALCAAAIGGLGIVNEPDFLLSEAIRHGSLVRLLPDYDGAGGEIWAVYPSRR
                     HLSLKVRLFVDHIAQAFAASGARTGAVSASQQ"
     gene            2216..2644
                     /locus_tag="VLN25_00165"
     CDS             2216..2644
                     /locus_tag="VLN25_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HSQ09021.1"
                     /translation="MIDSRTAPYAALLLRLALGSMFVAHALLKLFVFTLPGTVQFFQS
                     LGLPGALAYVTFAAELVGGVMLIFGVGTRWVAAALLPFLIGAAWVHLGNGWLFSAPKG
                     GWEYPVFLIVAAIAQTLLGDGAYALGTRFGGRSSVRLQTA"
     gene            2672..>3028
                     /locus_tag="VLN25_00170"
     CDS             2672..>3028
                     /locus_tag="VLN25_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HSQ09022.1"
                     /translation="MKLYYSPGACSLSPHIVARAAGLPLSLVRVDTKARKTAAGDDYR
                     AINGKAYVPMLELEDGTRLTEGPAIVQYLADRAPEKRLAPANGTMERYKLQEWLNFIT
                     SEIHKQFSPLFDAGMPD"
BASE COUNT          511 a         1019 c          935 g          563 t
ORIGIN      
        1 atacgcttcc ttgaagccct ttggtgtctt taccgccttg ctcgtcgggt aaaaagtgca
       61 gccttcaata tcgccgtcca tgttggtcgg cagcagccac tcctcgcaga acttgcccgc
      121 ctcctcaacg acatggttca ccgtttgaac atcgagatcg gcgtaacgcg gaacctcgtt
      181 gaattcaccg acggcaccca ggacttcgtg cagcacgaac tgttgatcgc gcaatggcgc
      241 tttgtagata ggcatgttta ctccttggct gcgcgcgggc gcgaccgtgc aggtcgggcc
      301 gtgacacttt tttgacgggt tctggaggtg cgctgtgaac ggctcgcggg ccgtcccggt
      361 ttcggcggcg cggcgcgctt gcgcatgccc gcacgggttt tgggttcgct acgagtcgcc
      421 tttgccgcgg acgcgagcag gcgtgcgaga ccggcgcgtg cgcgcttggc actgtcggcc
      481 gagcggagca ggcgcgcatc ctggtgcatg gccagcatca gtccgtagat ctcgaacacc
      541 atttgcggcg catcgaccgt agcgctcagg tgtccttcgg tcaccgcctg ccgtatggtc
      601 ttcagcaact cggccttcca gccggtgatg attgcgacca tcgcgtcgcg ctgcggaccc
      661 ggccggtcgt cgtactcgac cgcgccggca atcatgatgc agccgcgcgt cagttctcgc
      721 gcaaggaagg cgacccatcg atcaaggatc gcctcgagcc ggggcaggcc gcgcggcttc
      781 ttcacggccg gtcgcaggac ctcgtcgatg aagcggctca cgtatgcctt cagcacggcc
      841 agctgcaggt cctcgcgcga gccaaagtgc gcgaatactc cgctcttgct catgtgcatc
      901 tgatcggcca gtgagccgat cgtcaggccc tccaggccct cgcggctcgc cgtctcgagc
      961 gccgcgtcga cgatcgccgc acgcgtctgg tcgcccttgc gcggacgcag ggcggtggcg
     1021 atcgacgaca gggcggtagc gatgggcatc agggaagcct gccggcagcg catggcggcg
     1081 gcatgacata aaaacgaacg ttcgtactat ttccgacgcg acaccccccg tcaaccgggg
     1141 gtctagagga tcggacctat tgctgcgacg cagaaacggc gccggtccgg gcaccggacg
     1201 ccgcaaaggc ctgcgcgatg tgatcgacga acaggcgcac tttcagcgac agatgtcgcc
     1261 ggctcggata caccgcccag atttcgccgc cggcgccgtc atagtcgggc agcaggcgca
     1321 ccaggctccc gtggcgtatg gcttcgctca agaggaaatc cggctcatta acgatgccga
     1381 gccctccgat tgccgccgcg cacagcgcat caccgctgtt cgcgtgcagg ctcccagtca
     1441 cccgaacctc gtgcgcacgg ccgccggtgt ccgtaaagcg ccacagccgc gggctgctcg
     1501 agtaggcata ggtcagtgca ttgtgtttcg ccagctcgcc gggagcacgc ggcaccccgt
     1561 gcctttccag ataagccggc gcggcgcaca ggagcaactg catggcaccc agtcgccgcg
     1621 caaccaggcg gtcgctgccc atctgtccga cccggatcgc gaggtcgaat ccctcctcga
     1681 ccaggtcgac catccgatcc gacaccacga gttcgaaccg cacctcggca tggcgttcga
     1741 caaagctcgc aatcgcgggc gcgacccgtt gctcctccat gctgtgcgaa cacgtgagac
     1801 gtacggttcc acgcggctgt gcggacgcct gcccggcgat gtcctcggct tcggccaggt
     1861 cggccagcaa ggtcacacag cgttcgtagt acgactgccc gatctcggtc agggaaaggc
     1921 ggcgggtcgt ccggttgagc agccgcgcgc cgagatgatg ttcgagctcg ccgacgagcc
     1981 gggacagtga cgaggtggac agtccgagcc gctccgcggc cgcgacgaag ctcccggact
     2041 cgacaacccg tacgaaggcc tccatcccat gccagcgatc cacgacgaca ttatcctgat
     2101 tttcaggaca atatctttct ggattgacta tttatcttgc tttctgggat ggctaaagtt
     2161 tgtccgtcgg ccccgggttc gccgggaccc agtcaacccc gataggagaa ctccaatgat
     2221 cgatagtcgc accgccccct acgccgccct gctgctgcgc ctggcgctgg gctccatgtt
     2281 cgtcgcccac gccctgctga agctcttcgt attcacgctt cccggtaccg tccagttctt
     2341 ccagtcgctc ggtttgcccg gggcacttgc ctatgtgacg ttcgccgccg aactggtcgg
     2401 tggggtcatg ctcatttttg gagtcggaac acgctgggtg gccgcggccc tgttgccctt
     2461 cctgatcggt gccgcctggg ttcacctcgg caacggctgg ctcttcagtg cgccgaaggg
     2521 cggatgggaa tacccggtgt tcctgattgt cgcggccatt gcccagacgc tgcttggcga
     2581 tggtgcctac gccctgggta ctcgcttcgg cggtcgctcg tccgtgcgcc tacaaaccgc
     2641 ctgatccgaa cactcaacag gagaaaaccg catgaagctc tattactccc cgggcgcctg
     2701 ctcgctgtcg ccccacatcg tcgcgcgtgc cgccggtctg ccactgtcgc tggtgcgggt
     2761 cgacaccaag gccaggaaga cggccgctgg cgacgactac cgcgccatca acggcaaggc
     2821 ctacgtgccc atgcttgaac tcgaggacgg cacgcgcctg accgaaggtc cggcgatcgt
     2881 ccagtatctg gctgacaggg caccggaaaa gcggctcgca cccgccaacg gcacgatgga
     2941 gcggtacaaa ctgcaggagt ggctcaactt catcacctcc gagatccaca agcagttctc
     3001 gccgctgttc gatgcgggca tgccggat
//
LOCUS       DATIRT010000007         2930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_688979, whole genome shotgun sequence.
ACCESSION   DATIRT010000007 DATIRT010000000
VERSION     DATIRT010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688979"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VLN25_00175"
     CDS             <1..297
                     /locus_tag="VLN25_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09023.1"
                     /translation="GKERDRVERLWLITDKSPLSTVLMRDYEGTHFVRVRAEDMRAFL
                     ATDGTNPEGHVWLIDPMGNLMLRWPQDPDPQRVKKDLARLLTASRHWVRIERKD"
     gene            297..1376
                     /locus_tag="VLN25_00180"
     CDS             297..1376
                     /locus_tag="VLN25_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004543853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HSQ09024.1"
                     /translation="MDTVALAWLAAKGIGVAAIPLAYVALSRDPAKFRKLAWVTAFLT
                     LDLIMFGSFTRLTDSGLGCPDWPGCYGHSNPLSAGEPIRAAESAQPTGPVTMTKAWIE
                     MLHRYFAMGIGLLIIILAVQAWRRVRIDATAPSPWLATAALIWVCVQGAFGALTVTMK
                     LQPVIVTAHLLGGVALLALLVWLAMRESPEPPVAATARRLRLLAAAAMSVVVVQIALG
                     GWVSSNYAVLACTDFPRCQGAWIPPMDFDHGFTLWRALGMTGDGEVIPFQALVAIHWV
                     HRAFALVVVGVVASLIWRAWSLVGMTRVARGLLAILLLQVATGLSNIVLEWPLLAAVL
                     HSGGAAMLVALLVIANVRAARAARL"
     gene            1411..2304
                     /gene="cyoE"
                     /locus_tag="VLN25_00185"
     CDS             1411..2304
                     /gene="cyoE"
                     /locus_tag="VLN25_00185"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HSQ09025.1"
                     /translation="MRGQILATQRSLGLIASQYWALTKPRVTQLAIFCAVIGMFLATS
                     ELPSARTVAMATLGIWLLAGAAFALNSLIERHIDARMARTRMRPLPQGDLTPLQALAF
                     SGVIGGVGMWVLHTQVNPLTMWLTFATFVGYAVVYTVLLKPYTPQNIVIGGMSGAMPP
                     VLGWAAVTDSAPPEAWLLALIIFVWTPPHFWALALYRIDDYRKAGWPMLPVTHGEPLT
                     RLHILLYTIGLVATTLLPYAVQMSGMLYLVSALALGAVFVAYAWRLFRSPNAALARAT
                     FNYSIVYLAALFAALLVDHYV"
     gene            2325..2915
                     /locus_tag="VLN25_00190"
     CDS             2325..2915
                     /locus_tag="VLN25_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HSQ09026.1"
                     /translation="MPMIVLPRWLAVFVGAVWAFALTACSPPTFKAVDITGAPYANNF
                     SLNAAGGGRRTLADFRGKVVAVFFGFTQCPDVCPTTLSDLAEVRKRLGADGDRLQVVF
                     ITIDPERDTPALLSEYGPGFDPTFIGLYGTAEETALTAKEFKVFYQKVPGKTATSYTM
                     DHTAGSFVFDKEGRVRLFIRHGASVDDITADLRQLL"
BASE COUNT          428 a          949 c          948 g          605 t
ORIGIN      
        1 ggcaaggagc gcgaccgcgt tgaacgcctg tggctcatca ccgacaagtc accgctctcg
       61 accgtgttga tgcgcgatta cgaaggaaca cacttcgtgc gcgtgcgcgc tgaggatatg
      121 cgcgcatttc tggctacgga cggaacaaat ccggagggtc atgtctggct gatcgatccg
      181 atgggaaacc tgatgctgcg ctggccgcag gacccggatc cgcagcgggt gaagaaggac
      241 ctggcgcggc tgctgaccgc atcgcgccac tgggtacgta tcgagcgcaa ggactgatgg
      301 acaccgttgc actggcctgg ctggcagcaa agggcattgg cgtcgcggcc attccgctgg
      361 cttatgtcgc gttgtcgcgc gacccagcca agttccgcaa gctggcctgg gtcacggcgt
      421 ttctgacgct cgacctcatc atgttcggca gctttacgcg cctcacggac tcgggtctcg
      481 gctgcccgga ctggccgggc tgctacggcc actccaaccc gctatcagcc ggtgaaccga
      541 tccgcgctgc cgaatcggcg caaccgaccg gcccggtcac catgaccaag gcctggatcg
      601 agatgcttca tcgctacttt gcgatgggca tcggactgct gatcatcatt cttgccgttc
      661 aggcatggcg ccgggtccgg atcgacgcca cggcgccgtc gccgtggctg gcgacggccg
      721 cgctgatctg ggtctgcgtg cagggagcgt tcggggcctt gacggtgacg atgaagctgc
      781 agccggtcat cgtcaccgct catctgctcg gcggtgttgc gctgctcgca ttgctcgtct
      841 ggctcgcgat gcgcgaaagc cccgaacctc cggtcgcagc cacggcgcgc cggctgcggc
      901 tcctggccgc cgcggccatg tcggtggtgg ttgtacagat cgcgctcggc ggttgggtca
      961 gcagcaacta cgcggtcctc gcctgcaccg attttccgcg ttgccagggc gcctggattc
     1021 cgccgatgga cttcgaccac ggattcacgc tgtggcgtgc gctcgggatg accggggacg
     1081 gagaagtcat tccattccag gcgctggtcg cgatccactg ggtccatcgg gcctttgcac
     1141 tggtcgttgt cggagtcgtc gccagcctga tctggcgcgc gtggtcgctc gtcggtatga
     1201 cgcgggttgc gcgcgggttg ctggcgatcc tgctgctgca ggtcgccact ggactctcga
     1261 acatcgttct cgagtggcca ctgctggcgg ctgtcctcca cagcggcggt gcagcaatgc
     1321 tggtggcgct gctggtcatc gcgaatgtcc gtgcggcgcg tgcggctcga ctgtaagatt
     1381 cccgactcga gtcgacagca cctgaccatg atgcgcggcc agattctcgc cacccagcgt
     1441 tcactgggcc ttattgcttc ccagtactgg gcgctgacca aaccgcgcgt gacccagctt
     1501 gccattttct gcgccgtcat cggcatgttt ctggccacat cggaactgcc gtcggccagg
     1561 acggtggcca tggccactct tggaatctgg ctgctggcag gtgccgcctt cgcgctgaat
     1621 tccctgatcg agcgacacat cgacgcgagg atggcgcgca cgcggatgcg tccattgccg
     1681 cagggtgacc tgacgcccct gcaggcgctg gccttctcag gcgtgatcgg tggcgttggc
     1741 atgtgggtcc tgcatacgca agtcaatccg ctgacgatgt ggctcacgtt cgccactttc
     1801 gttggatacg cagtcgtgta cacggtgctg ctcaagccgt acacgccgca gaacatcgtg
     1861 atcggcggca tgagtggcgc tatgccgccg gtgctcggct gggctgcagt cacggacagc
     1921 gcgccgccgg aggcctggct gctcgcgttg atcatcttcg tctggacgcc gccgcacttc
     1981 tgggcgttgg ccctgtatcg gatcgatgac taccgcaagg ccggctggcc gatgctgccg
     2041 gtcacgcatg gcgagccgct gacccgtctg cacatcctgc tgtacacgat cggcctggtg
     2101 gccacgacgc tgctgcccta tgccgtgcag atgtcgggaa tgctttacct ggtgtcggcg
     2161 ctcgcgctcg gggcggtgtt cgtcgcttac gcgtggaggc tcttccgttc tccaaacgcc
     2221 gccttggcgc gtgccacctt caattactcg atcgtgtacc tggcagcgct cttcgcggcc
     2281 ctgctggtcg atcactacgt ctgacctgcc gcggcgacga tccgatgccg atgattgtcc
     2341 tgccgcgctg gctcgcggtt ttcgtcggtg ccgtgtgggc gttcgcgttg accgcctgct
     2401 cgccgccgac ctttaaggcg gtcgacatca cgggcgctcc ctacgcgaat aacttttcgc
     2461 tcaacgccgc cggtgggggt cgtcgtacgc tggccgactt ccgtggcaag gtcgtggcgg
     2521 tcttcttcgg cttcactcag tgtcccgatg tctgcccgac cacgttgtcg gatctcgctg
     2581 aagtcaggaa gcgtctgggg gctgacggcg accggctgca ggtcgtgttc atcacgatcg
     2641 atcccgagcg cgataccccc gccctgcttt cggagtacgg acccggcttc gatccgacct
     2701 tcattgggct gtacggcacc gccgaagaga cggcgctgac tgccaaggag ttcaaggtgt
     2761 tctaccagaa ggtgcccggc aagacggcca cgtcctacac gatggaccat accgcgggct
     2821 cctttgtctt cgacaaggaa ggccgcgtgc gcctgttcat ccgccacggc gcgagtgtcg
     2881 acgacatcac ggccgatctg cgtcagctcc tctaggctgt tcagcgctca
//
LOCUS       DATIRT010000008         1956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1869077, whole genome shotgun sequence.
ACCESSION   DATIRT010000008 DATIRT010000000
VERSION     DATIRT010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1869077"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VLN25_00195"
     CDS             complement(<1..579)
                     /locus_tag="VLN25_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HSQ09027.1"
                     /translation="MAAFDMSVWQGRVDDAEGALGRRWHQMMQPLASGTAPATTVLLG
                     FACDAGVARNHGRIGAAEGPRAIRRALANMPVHECTHLADGGDVLCAGDALEAAQHAL
                     AAAVAQQLDAERFPLVLGGGHEMAWGSFTGLATHLAMTVEAPRVGIVNFDAHFDLRRD
                     SRATSGTPFLQIAQDCEARGWEFRYACFGVSRY"
     gene            complement(588..>1956)
                     /gene="hutH"
                     /locus_tag="VLN25_00200"
     CDS             complement(588..>1956)
                     /gene="hutH"
                     /locus_tag="VLN25_00200"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HSQ09028.1"
                     /translation="GLLAKKRIPDAELQQLQRNLILSHSVGTGALLRDDVVRLALVMK
                     IGSLARGHSGVRPLIVEALIALVNAGIVPCIPSKGSVGASGDLAPLSHLTLALLGEGD
                     VRVNGAQQPARSALAAAGITPVVLGAKEGLALINGTQISTALALHGLFMAERVFEAAV
                     VVGAMAIDAAKGSDSPFDPRLHELRGHAGQIATANAFRQLLAGSAIRASHLVDDDRVQ
                     DPYSLRCQPQVMGACRDLIVQAARTLLTEANAVTDNPLVFARRDTNAADEVLSGGNFH
                     AEPVAFAADTLALAIAEIGALSERRIALLIDATLSGLPPFLVRDPGVNSGFMIAHVTA
                     AALASENKSLSHPASVDSLPTSANQEDHVSMATFAARRLHEMATNTATIVGIELLAAA
                     QGIDFHRPLATSPRLAAVHRGLRKRVPFYEVDRILAPDITAATELVAAGALSAGCRDL
                     FAPLA"
BASE COUNT          325 a          673 c          666 g          292 t
ORIGIN      
        1 atagcggctg acgccgaagc aggcgtaacg gaactcccag ccgcgcgcct cgcaatcctg
       61 cgcgatctgc aggaacggtg tgccggaggt ggcgcggctg tcgcgccgca ggtcgaagtg
      121 cgcgtcgaag ttgacgatgc ccacgcgcgg cgcctccacg gtcatcgcaa ggtgcgtggc
      181 gagccccgtg aacgaccccc aggccatctc gtggccgcct ccgaggacca gcgggaagcg
      241 ctcggcgtcg agctgctgcg ccaccgcggc cgcaagcgca tgttgtgccg cctcgagcgc
      301 gtcgcccgca cataggacat cgccgccgtc ggcaaggtgt gtgcactcgt gcaccggcat
      361 gttggcgaga gcacgacgga tcgcgcgggg accttctgcc gcgccgatgc gaccgtgatt
      421 gcgcgcaacc cccgcatcgc acgcgaagcc cagcagcacc gttgtcgccg gcgcggtgcc
      481 tgacgcaagc ggctgcatca tctggtgcca gcgccgtccg agcgccccct cggcgtcatc
      541 aacgcggccc tgccacaccg acatgtcaaa ggctgccatt cagcgagtca tgctagcggt
      601 gcgaagagat cacggcagcc ggcgctgagc gctcccgcgg ccaccagctc ggtggcggcg
      661 gtgatgtcgg gcgccaggat gcgatcgacc tcgtagaacg gcacgcgctt gcgcaagccc
      721 cgatgcactg cggcgaggcg cggcgaggtc gcgagtggtc gatgaaaatc gatgccctgg
      781 gctgccgcga gcaactcgat gccaacgatg gtcgctgtgt tggtcgccat ctcgtgcaaa
      841 cggcgcgcgg caaacgtcgc catgctgacg tgatcctcct ggttggccga ggtcgggagg
      901 ctgtccacac tcgccggatg cgagagcgac ttgttttcgg aggccagcgc cgccgcggtc
      961 acgtgcgcga tcatgaagcc cgaatttacg ccggggtcgc gcaccaggaa tggcggcagg
     1021 cccgacagcg tggcgtcgat caaaagcgca atgcgacgct ccgacagcgc accgatctcc
     1081 gcgatcgcga gcgccagcgt gtcggccgcg aacgccaccg gttcggcgtg gaagttgcca
     1141 cccgacagga cctcatcggc cgcgttcgtg tcgcgccgcg cgaaaaccag cgggttgtcg
     1201 gtgacggcgt tggcttcggt cagcagtgtg cgcgcggcct gcacaatcag gtcacgacat
     1261 gctcccatca cctgcggctg acagcgcagg ctgtacggat cctgcacgcg atcgtcatcg
     1321 acgagatgtg aggcgcggat cgcgctgccg gccagcaatt ggcggaaggc gtttgcggtc
     1381 gcgatctggc ccgcatgccc gcgcagttcg tgcagacgcg gatcgaacgg cgagtcgctg
     1441 ccctttgccg cgtcgatcgc catcgcgccg accactaccg cggcttcgaa cacgcgctcc
     1501 gccatgaaca gcccatgcag cgccagcgcg gtggaaatct gcgtgccgtt aatcagcgcc
     1561 aatccctcct tcgcaccgag cacgaccggc gtgatgccgg cggcggccag tgccgaccgc
     1621 gccggctgtt gcgcgccgtt gacgcgtacg tcgccctcgc ccagcagcgc gagcgtcaga
     1681 tgcgacagcg gtgcgaggtc gcccgaagcc ccgaccgagc ccttcgaggg gatgcacggg
     1741 acgatgccgg cgttgacgag tgcgatcagc gcttcgacga tcagcggccg cacaccagag
     1801 tgaccccgcg caaggctgcc gattttcatc accagcgcca ggcgcacgac gtcatcgcgc
     1861 agcagtgcgc cggtgccgac cgagtgcgac aggatcaggt tgcgctgcaa ttgctgcagt
     1921 tctgcgtccg gaatgcgctt cttcgcgagc aggccg
//
LOCUS       DATIRT010000009         1804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1869187, whole genome shotgun sequence.
ACCESSION   DATIRT010000009 DATIRT010000000
VERSION     DATIRT010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1869187"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /locus_tag="VLN25_00205"
     CDS             complement(<1..1233)
                     /locus_tag="VLN25_00205"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HSQ09029.1"
                     /translation="MQAAGGSLVLALTPVHIARGATLLGVRVWPARDYTRVALEHDEP
                     LKFTHFLIRDTAPLRMVVDVEGIDLTPAFREGVAKVEPNDPYIGVIRVGQNRPKVVRL
                     VIELKEDIKPQVFSLEPVGPYQHRLVMDLYPVNPPDPLMALLREQATPRDSDFSSVLP
                     SPPLATDRAGDRAVDQRMARAPTRPTRRRSDSDVQRIVTVAIDPGHGGEDPGAVGKRG
                     TYEKTVTLSIARRLREMIVADSGMRAALTRDGDYFVPLRTRVSKARAVRADLFVSIHA
                     DAWIRPDARGSSVFALSENGASSSTAAWMARRENDADLIGGINLASHDKQLARVLLDL
                     STTAQISDSLKLGSSVLRELERVNRLHKPRVEQAGFAVLKAPDIPSILVETAFISNPD
                     EEARLRDDDYQEQMARAML"
     gene            complement(1233..>1804)
                     /locus_tag="VLN25_00210"
     CDS             complement(1233..>1804)
                     /locus_tag="VLN25_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008540850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HSQ09030.1"
                     /translation="ESFTAQIVRRLPMPADTDRLGRALATACRDHAAAIAEHGLQVNL
                     SGDLGAGKTALVRGWLRAFGISGPIRSPTFAVLEPYVVSLSRPGAAGHSGLEAKDISS
                     LDFYHFDFYRFADPSEFSIGFRDLFGPRCICAIEWPEKAGERLPTADVAIALQVEDAG
                     RRAILIASGELGRACLESTAKEFDTTAAA"
BASE COUNT          296 a          595 c          604 g          309 t
ORIGIN      
        1 gagcatggcc cgcgccatct gctcctggta gtcgtcgtca cgcaagcgcg cttcctcgtc
       61 cgggttgctg atgaaggcgg tctcgaccag gatcgacgga atatccggtg ccttcagcac
      121 cgcgaatccg gcctgttcga cgcgcggctt gtgcagccgg ttgacgcgct cgagttcgcg
      181 cagcaccgag ctgcccagct tgaggctgtc gctgatctgg gcggtggtcg acaggtccag
      241 cagcacgcgc gccagctgct tgtcgtgcga cgcgaggttg atgccgccga tcaggtcggc
      301 gtcgttctcg cgccgcgcca tccatgcggc agtgctactc gacgcgccat tttccgacaa
      361 tgcgaacacc gacgagcccc gcgcgtccgg ccggatccag gcatcggcgt ggatcgagac
      421 gaagagatcc gcccgcactg cccgcgcttt cgacacgcgc gtgcgcaagg gaacgaagta
      481 atcgccgtcg cgcgtcagcg ccgcgcgcat gccgctgtcg gcgacgatca tctcgcgcaa
      541 gcggcgcgca atcgacaacg tcacggtctt ctcataggtt ccgcgcttgc cgacggctcc
      601 cgggtcttcg ccgccgtggc ccgggtcgat cgccaccgtg acgatgcgct ggacatccga
      661 gtcgctgcgc cgacgcgtcg ggcgcgtagg tgcgcgcgcc atcctctggt caaccgcgcg
      721 gtcgcccgcg cggtcggtcg ccagtggcgg cgacggcagc accgagctga agtccgagtc
      781 gcgcggagtg gcctgttcgc ggagcagcgc catcagcggg tcgggcgggt tgaccgggta
      841 aaggtccatc accagtcgat gctgataggg cccgaccggt tcgagcgaga agacctgcgg
      901 cttgatgtct tccttcagct cgatgaccag gcgcaccacc ttgggccggt tctggcccac
      961 gcggatcact ccgatgtaag gatcgttcgg ttcgaccttg gcaacgcctt cacggaacgc
     1021 cggtgtcagg tcgatgcctt cgacgtcgac caccatgcgc agcggcgctg tgtcgcggat
     1081 caggaagtgc gtgaacttga gcggttcgtc gtgttccagt gccacgcgcg tgtagtcgcg
     1141 ggcgggccag acgcgcactc cgagcagcgt ggcaccgcgt gcgatatgga cgggagtcag
     1201 cgcgagcacg agcgagccgc ccgcggcctg catcaggcgg cggcggtcgt gtcgaactcc
     1261 ttggctgtgc tctccaggca tgcgcgcccc agttcaccgg acgcgatcag gatcgcgcga
     1321 cgtccggcgt cttcgacttg cagggcgatc gccacgtccg cagtgggcag gcgttcgccg
     1381 gctttctcgg gccattcaat tgcacagatg caacgcggcc cgaacaggtc tcgaaagccg
     1441 atcgaaaatt ctgaaggatc ggcaaatcga taaaaatcaa agtgatagaa gtctaagctc
     1501 gaaatgtctt tagcttcaag tccggagtga ccggcggcgc ccggtcgact caacgacacc
     1561 acatagggct ccagcaccgc gaaggtcgga ctgcggatcg gcccgctgat gccgaaggcg
     1621 cgcagccatc cgcgtaccag cgctgtcttg ccggcgccga gatcaccgct caggttgacc
     1681 tgcagaccgt gctcggcgat ggccgccgca tggtcgcggc aggccgttgc cagcgcccga
     1741 ccgagccgat cggtgtcagc gggcatgggc agcctgcgca cgatctgcgc cgtgaaggat
     1801 tctt
//
LOCUS       DATIRT010000010         2067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1770946, whole genome shotgun sequence.
ACCESSION   DATIRT010000010 DATIRT010000000
VERSION     DATIRT010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1770946"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            360..1295
                     /locus_tag="VLN25_00215"
     CDS             360..1295
                     /locus_tag="VLN25_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HSQ09031.1"
                     /translation="MATSLLALLDDIASVLDDVATLTKVATRKTAGVLGDDLALNAEQ
                     VSGVRPDRELPVVWAVAKGSLVNKAILVPAALAISAVAPWAIVPLLIVGGLFLCYEGF
                     EKLAHKFLQSADDKAARATELAQALLDPAVDLVALEKDKIKGAIRTDFILSAEIIVIT
                     LGTVAGAAFGTRLAVLVGIALLMTVGVYGLVAGIVKLDDGGLLLTRASGDHGWARFKR
                     ALGRGILRGAPWLMKGLSVAGTAAMFLVGGGILTHGIPPLRDAIEHLLVGLGALMQAL
                     GPLLIDAVVGVIAGALTLAVVSGVKRLRGAPAAHH"
     gene            complement(1391..1738)
                     /locus_tag="VLN25_00220"
     CDS             complement(1391..1738)
                     /locus_tag="VLN25_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09032.1"
                     /translation="MRTFARLVCCALLVAIPAHHALAQRAGDGDRAGRRAQGGGINYA
                     PQPDVYSVISIDSYARTVRMRDADGRIGDVHVADSVYDLSTLKAGDKIRVDFVVPDSM
                     NSRLSAASVWLEK"
     gene            complement(1826..>2067)
                     /locus_tag="VLN25_00225"
     CDS             complement(1826..>2067)
                     /locus_tag="VLN25_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSQ09033.1"
                     /translation="LRRFMPAAEGLARSSAVCLYTNTPDEHFLIDRHPGEPRVLVASP
                     CSGHGFKFAPVVAEILADLVQDRPQSFDLALFRWR"
BASE COUNT          337 a          677 c          687 g          366 t
ORIGIN      
        1 ggtcgtggcc gttctgacca gtgccgtggt gctgggcagc accgtggccg gcatgcagcc
       61 gcgcgacgac gctggcctgc aggtcatcgc actcgagcgc gtgaccgtga cagcgccgcg
      121 gatcaactga ggatcgactg aacgaagcac gccgcgcccg gtcccgcggc cgcccgcgtg
      181 cagggcacac gagacactgc acactgcatc aaaggaaagc ccggcttcgg ccgggctttc
      241 tgtttctgac tccatggcgg tccggcagac cgctatcgtt cggcctgctg aaagtctcgc
      301 cgccaggctg ccgagtagcc gtgtcgtccg gacttccctt caaaaccgga ctcccgccca
      361 tggcaaccag cctgctcgcc ctgctcgatg acatcgccag cgtgctcgac gatgtcgcca
      421 cactgaccaa ggttgcgacc cgcaagaccg ctggggtact gggcgacgac ctcgcactga
      481 acgccgagca ggtgtccggc gtgcgcccgg accgggaact gccggtggtc tgggcagtgg
      541 ccaagggttc gctggtgaac aaggcgatcc tggtgccggc cgcgctggcg atcagtgcgg
      601 tcgcgccctg ggccatcgtg ccgctgctga tcgtcggggg actgttcctt tgctacgagg
      661 gtttcgaaaa acttgcccac aagttccttc agagcgccga cgacaaagct gcacgggcga
      721 ccgaactggc acaggcactg ctcgaccctg cagtcgacct ggtggcgctg gagaaggaca
      781 agatcaaggg tgcaatccgt accgacttca tcctgtcggc tgaaatcatc gtaatcaccc
      841 tgggcaccgt cgccggcgcg gcgttcggga cgcgactggc cgtgctggtc ggcatcgcac
      901 tgttgatgac cgtcggtgtg tatggactcg ttgccggcat cgtcaagctc gacgacggag
      961 ggctgctgct gacgcgcgca tcgggtgacc acggctgggc gcgtttcaag cgcgcgctcg
     1021 gtcgcggcat cttgcgcggc gcgccctggc tgatgaaggg gctgtcggtg gctggcaccg
     1081 cggccatgtt cctggtcggc ggcggcatcc tcacccatgg cattccgccg ctgcgcgatg
     1141 cgatcgagca cctgctcgtg ggcttgggcg cgctgatgca ggcgctcggg ccgctgctga
     1201 tcgacgcggt ggtcggtgtg attgccggcg cgctgacgct cgccgtcgtg agtggcgtca
     1261 agcgattgcg cggtgcaccc gcggcccacc actgacgcag aaggtcgcac gctggccgaa
     1321 ggccgtcacc tcgctggcgc gtatcggcca acgagcgcca tggtggagtc tctcggcttc
     1381 agcaagtcac tcacttctcc agccaaacgg acgctgcact cagcctgctg ttcatcgagt
     1441 cgggcacgac gaagtcgacc cggatcttgt cgcccgcctt cagcgtcgac aggtcgtaga
     1501 cgctgtccgc cacgtggaca tctccgattc ttccatcggc gtcacgcatg cggaccgtgc
     1561 gggcatagga gtcgatggag atgacagagt acacatcagg ctgcggcgca tagttgatgc
     1621 cgccgccctg tgcgcgccga ccggcacggt cgccgtcgcc tgctcgctgc gcgagcgcgt
     1681 ggtgtgccgg aatggcgacc agcagcgcgc agcagaccag ccgggcaaat gtcctcattg
     1741 ttcgcctccc tgtgatggtt cgaaatctac cgcgtcctgc ttgctgcagg attgtcgacg
     1801 cgcatccacg ttcacccatg cgactttagc gccaacgaaa aagagccagg tcgaagcttt
     1861 gtggcctgtc ctgtaccaga tctgccagga tttccgcgac gaccggggcg aacttgaagc
     1921 cgtgtcccga acacgggctg gccacgagga cgcgcggttc gcctggatgc cggtcgatca
     1981 agaagtgttc gtcgggcgtg ttcgtgtaca ggcagacggc ggacgaccgc gccaagccct
     2041 cagcggccgg catgaatcgc cgcagcg
//
LOCUS       DATIRT010000011         2095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_984543, whole genome shotgun sequence.
ACCESSION   DATIRT010000011 DATIRT010000000
VERSION     DATIRT010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984543"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VLN25_00230"
     CDS             <1..636
                     /locus_tag="VLN25_00230"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HSQ09034.1"
                     /translation="LGIEQVIRPGEVNWMTSGWGISHSERFDGMRNRGGRLHGLQAWV
                     AFPVVHEEVEPSFEHYGRGELQDIDAPGMRARLIAGSAFGATSNVRTHSPLFYAHLEL
                     EAGTRAEMPSGYSERAAYVVSGSVETAGRVVGARTMVVFSAASVPVLSALEPSTVMVL
                     GGEPVGARHVWWNFVSSRKERIEQAKRDWADGRFALPPGDGAEWIPLPEGA"
     gene            710..1201
                     /locus_tag="VLN25_00235"
     CDS             710..1201
                     /locus_tag="VLN25_00235"
                     /inference="COORDINATES: protein motif:HMM:NF039279.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09035.1"
                     /translation="MGCLSFILPPRLGSAELVVIQPNSALTGRRFAIKFLLRSYKLSR
                     QNIYAEQSIPGLPGAPLQFLRGQPKVLSMVMYFDARHENSDVRELTNDIARLMKVDRA
                     SHAPPVLRFEWKGLSLQCVLESATEEIISLFPDGRPARAKMHATFKELRTLAELQEEL
                     DRE"
     gene            complement(1290..1700)
                     /locus_tag="VLN25_00240"
     CDS             complement(1290..1700)
                     /locus_tag="VLN25_00240"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HSQ09036.1"
                     /translation="MIAAAHAATVTNPAERRRLARERLRRLARLMDAAIEVPFLRTTV
                     GMDAALGIVPVVGDLVSAAIGLYAVALARELGASGWLQTRMVGNLLADAAIGALPVAG
                     DIADLYFRAHLRNLKLLQKELGEPFIDVTTRSGS"
     gene            complement(1738..>2095)
                     /locus_tag="VLN25_00245"
     CDS             complement(1738..>2095)
                     /locus_tag="VLN25_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016355425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HSQ09037.1"
                     /translation="PDLKGIETMRKYNEQLQKAGVLLALDGLTPPATMSARVTFKSGN
                     SKVTDGPFTEAKEVVGGYWVIQVKSREEALEWASRIPGRDSEMVEVRRLFDITDFPAD
                     VQKRLGELEEQSAEGG"
BASE COUNT          393 a          621 c          656 g          425 t
ORIGIN      
        1 ctgggaatcg agcaggtgat ccgaccggga gaagtgaact ggatgacgtc tggctggggc
       61 atcagtcact cggagcgctt cgacggcatg cgtaaccggg gcgggcgctt gcacggactg
      121 caggcctggg tggcgtttcc cgtggtgcac gaggaagttg aaccgtcgtt cgagcactac
      181 ggccgcgggg aactgcagga catcgacgca cccgggatgc gtgcgcgtct gatcgccggc
      241 tcggcgttcg gcgctaccag caacgttcgc actcattcgc cgctgttcta cgcacacctc
      301 gaactggaag ccggcacgcg ggccgaaatg ccgagcgggt attccgaacg tgcggcctat
      361 gtcgtcagcg gatcggtcga aaccgcgggc cgtgtggtcg gagcccggac catggtcgtg
      421 ttctcggccg cctcggtgcc tgtgttgtca gcgctcgagc cgtccacagt catggtgctg
      481 gggggcgaac cggtcggtgc gcgtcatgtc tggtggaatt tcgtgtcgtc gcgcaaggaa
      541 cgcatcgagc aggccaagcg ggactgggcg gacggccgtt tcgcattgcc tcccggtgac
      601 ggtgccgaat ggataccctt gcccgagggc gcgtagacgt tcaaatgggt aaggagttca
      661 actctgactc ggactccttc cgcgtcgctt tgcagcaagg agtgtttgta tgggatgcct
      721 gagctttatt cttccaccga ggcttggcag tgcagagctg gttgtcattc aaccgaatag
      781 cgcgctgacc ggacgcaggt ttgcgatcaa gttccttctc agaagctaca agctctcgag
      841 gcagaacatc tacgcagagc aatccatacc gggtttgccc ggtgcgccac tgcagttcct
      901 gaggggtcaa ccgaaagtat tgtcgatggt gatgtacttc gatgcgcgcc acgagaacag
      961 cgacgtgcgc gaattgacca acgacattgc gcggttgatg aaggtcgacc gtgcctcgca
     1021 tgcaccaccg gtgctgcggt tcgagtggaa aggactttcg ttgcagtgtg ttctggagag
     1081 cgcgacggag gaaatcattt cgctctttcc agacggacga cctgcccgcg caaagatgca
     1141 cgccaccttc aaggaattga gaacgctggc ggagctgcaa gaagaactgg atcgagagtg
     1201 aggtgtgcgt gacgctgggt tagcgtggca ctgctctcac gtcagatgag ctgtcgggca
     1261 cttcgcagcg agctaggcgg ggccttactc tatgagcctg acctcgtcgt cacatcgata
     1321 aacggttcgc cgagttcctt ctgcagcaac ttgagattgc gcaggtgcgc gcggaagtaa
     1381 agatcagcga tatcgcccgc gacgggcagt gcgcctatag ctgcatcagc caacaggttg
     1441 ccgaccattc gcgtctgcaa ccaacccgac gcgcccagct cgcgagccaa cgccacggca
     1501 tacagtccga tcgcggccga aaccaggtct cccactacag ggacgatccc gagcgctgca
     1561 tccattccca ccgtcgtgcg cagaaacggc acttcgatgg cggcatccat aaggcgcgcc
     1621 aggcgccgca gtcgttcgcg cgccaggcgc cgacgctctg caggattcgt cacagtcgct
     1681 gcatgggcag ctgcaatcac ggcattctcc gaaatgggca cattgctgac ccagcttcta
     1741 accgccctcg gcgctctgct cttcgagctc tccaagcctc ttctgaacgt cggcagggaa
     1801 gtcggtgatg tcaaagagcc ggcgaacttc gaccatttcg ctatcccttc cggggatgcg
     1861 cgaggcccat tcgagcgcct cttcgcgcga tttcacttga atcacccagt aaccgccgac
     1921 tacttccttc gcttcggtaa acgggccgtc ggtaactttc gaatttccgc ttttgaaggt
     1981 gacgcgcgcg ctcatcgtcg cgggcggggt cagaccgtcg agcgcgagca gcacgccggc
     2041 tttctgtagt tgctcgttgt acttcctcat ggtctcaatg cctttgaggt cgggt
//
LOCUS       DATIRT010000012         7035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1574661, whole genome shotgun sequence.
ACCESSION   DATIRT010000012 DATIRT010000000
VERSION     DATIRT010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7035
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1574661"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VLN25_00250"
     CDS             <1..393
                     /locus_tag="VLN25_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSQ09038.1"
                     /translation="TALTRQAIEDADRSHVEQLTSLGATPFMRGLLLGWDERYALLTV
                     LLAAFGRAISEVGAVMIVGGNIEGFTRVMTTAIALETSKGDLPLAIALGIVLLLVVLA
                     LNALIAAVRTWRERLDGAATQVARIAVA"
     gene            390..1142
                     /locus_tag="VLN25_00255"
     CDS             390..1142
                     /locus_tag="VLN25_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSQ09039.1"
                     /translation="MKLSDNPQGAFLNAQAESDAVHPMFELERVGVRYGTAWALQSVS
                     LSIGAGERVALVGPNGSGKTTLLRVLNGLIEPSAGTAQRPATTRQTFLFQRPFMLRTT
                     AQHNVALGMWLRGARWRDARKQADEALARVGLAALAQHSARTLSAGQQQRLALARAWV
                     LASDVMLLDEPTASMDSDARNKVETLLGEFTSGRSQTLIFSSHTLGQVRRLASRVICL
                     QDGRIQADLPIQEFLLRNDATESGLSIEGILN"
     gene            1199..1948
                     /locus_tag="VLN25_00260"
     CDS             1199..1948
                     /locus_tag="VLN25_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HSQ09040.1"
                     /translation="MAGAQSITLSSTTSTEQSGLFAHLLPQFKQAGGVDVKVVAVGTG
                     QAMDIARRGDADALLVHDQVAEEKFVAEGYGLKRHPVMYNDFVLVGPKTDPAAARGSD
                     IVAALGKISAGNAAFISRGDKSGTHSAESRYWTTAGLASQRGSGYRECGCGMGPALNI
                     AAASGAYVLTDRGTWLAFKNRGDLTVLVEADKRLFNQYGVIVVNPAKHPHVKVAQAQQ
                     FADWMISRAGQSAIAAYKIGGEQLFFPNAGE"
     gene            2084..3328
                     /gene="glp"
                     /locus_tag="VLN25_00265"
     CDS             2084..3328
                     /gene="glp"
                     /locus_tag="VLN25_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HSQ09041.1"
                     /translation="MKAATPTIADVRMHGFRKRSEVSAVFDWIDQAARRLGAESLALE
                     QATGRTLAAEVIAPLDVPGFDRAAMDGFALRGAETAGATDYNPLEFRVLGQAMPGQPF
                     TGELLPRAAVRIMTGAPVSAGVDAVVPAEYATETDGQIIVTRPVAPGQHVGHRGEDLR
                     AGSTALAAGRRLRPQDLGLAASLGLDHVTVVRQPRVRMLVTGNEVKAPGAPKGMYEVY
                     DANSYMLRGLIARDGGVLESHLRLGDDPDAIRAALLAPGADVILVSGGSIVGREDYAP
                     RLIAEIGELAIHGVAMRPSSPAGVGRIGETLVFLLPGNPVSCLCAYDFFAGRALRLIG
                     GRSPDWPHRSVQAVVARKIVSAIGRVDYCRVQLVDGRVEPIALSGASILSSTTRADGF
                     VIVPSNSEGYGPDAVVTAYLYD"
     gene            3343..5367
                     /locus_tag="VLN25_00270"
     CDS             3343..5367
                     /locus_tag="VLN25_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HSQ09042.1"
                     /translation="MSTSHESGAQGAEPIAGQSQFLNVVSRDEATARFREHLRLTPLG
                     EEFVALAEALGRVLARDVAADIDVPGFDRANVDGFAVQAQDTFGAMEEAPRTLRLNDE
                     VIAPGIVPNLPVTCGHATMIATGGMLPRGADAVLMVEHSELVGHADTRNIEVRRPLTG
                     GENVSYASTDVARGETVLRSGQRLTSREIGVLAALGRSECPVFRRPRVAIFSTGNEIV
                     APGDSLRPGAVYDSNAAIIGAAVEELGGIAVYLGVIADNERALAAALARGLESDVVVF
                     SGGTSKGAGDISYRIVHTLRNPGVVAHGVALKPGKPICLAVTEGKPVVILPGFPTSAI
                     FTFHEFLAPVIRAFAGLPPERRHTVPATLPMRVNSERGRTEYLLVGLVQGPDGYLAYP
                     MGKGSGSVTTFSGAYGFITIDQHTEILDAGSAVAVTLLGEQLEPADLVIIGSHCVGLD
                     LLVGKLTRQGIRAKPMIVGSQGGLAAARRGECDIAGIHLMDPATGEYNRPMLSDTLTL
                     VAGYGRMQGFVFRRGDGRFEGKSFKEAISAALGEPGCVMVNRNAGSGTRILIDKWLAQ
                     ERPTGYGTQTKSHNAVAVAIAQQRADWGIAIDTVARQYGLAFIAMQEEQYDFVVPTSR
                     LERPAVQAFLALLDDPEVRNALASLGFRLPQKSQRQPAGSARVPLTESDE"
     gene            5480..6037
                     /locus_tag="VLN25_00275"
     CDS             5480..6037
                     /locus_tag="VLN25_00275"
                     /inference="COORDINATES: protein motif:HMM:NF023363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3455 domain-containing protein"
                     /protein_id="HSQ09043.1"
                     /translation="MAIIAVLLTACASVGSGPGATGGPGKAGAVTPPSLGLFSSIKVP
                     SDREPTLQLSAQGVQIFRCEKRDGGFAWVFRQPEAQLSDASGKPVGRHGASFSFEHND
                     GSRLVASVAAYDEAPNAADLRWLLLTTRSYGKGAFDGVTHVQRVNTRGGMPPAKCDAA
                     RANQLLRVDFSADFVFYGIKSGAAG"
     gene            6156..6878
                     /locus_tag="VLN25_00280"
     CDS             6156..6878
                     /locus_tag="VLN25_00280"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HSQ09044.1"
                     /translation="MNPTASTVGAYLRDLPPDRRVAMARLCSMIRRSARGVRESMRYG
                     LAFYELDGPLFALESGRRHMSLYVAEKDVVLKHKDALQGVNANKSFIKFSDLNRLPLD
                     VVEKIVRDSVAARRQRLAQGTIPTQPELLKLWGINEEAAAPPATTVKLPVAKPAASMS
                     DAINAMASTVRDAMIAAIDMATGMPVTEKPKARKAGPSRTAAAKAHPAKRTTSRAATR
                     TTSTRNPPKKAAAKKAVSARRR"
BASE COUNT         1218 a         2299 c         2339 g         1179 t
ORIGIN      
        1 accgcgctca cgcgccaggc gatcgaggac gccgaccggt cgcatgtgga gcagctgacg
       61 tcgctggggg ccacgccgtt catgcggggc ctcctgctcg gctgggacga acgctatgcg
      121 ctgctgacag tactgctcgc cgcgttcgga cgcgcgattt cagaagtggg tgcggtgatg
      181 atcgtcggcg gcaacattga gggcttcacg cgcgtgatga ccacggcaat cgcactggag
      241 accagcaagg gtgacctgcc gctcgcgatt gcactgggca tcgtgctgct cctggtggtg
      301 ctggcactga acgccctgat cgccgccgtg cgcacctggc gcgaacggct ggacggcgcg
      361 gcgacgcagg tcgcgcggat cgcggtcgcg tgaaactgtc cgacaaccct cagggtgcgt
      421 ttctgaacgc ccaggccgaa agcgacgcgg tgcatccgat gttcgagctc gagcgggtcg
      481 gcgtgcgcta cggcacagca tgggcgcttc agtcggtaag cctctcgatc ggcgcgggtg
      541 aacgcgtcgc gctggtcggc cccaacggca gcggcaagac cacgctgcta cgcgtgctga
      601 acggactgat cgagcccagc gcggggacgg cgcagcggcc ggcgacaacg cgacagacat
      661 tcctgttcca gcgccccttc atgctccgca cgaccgcaca gcacaatgtc gcgctgggca
      721 tgtggttgcg cggcgcacgc tggcgcgatg cccgcaagca ggccgacgag gcgttggcgc
      781 gggtcggtct ggctgcgctc gcccagcaca gcgcgcgcac cttgtccgcc ggacagcagc
      841 agcggctggc gctggcacga gcgtgggtgc tggcttccga tgtgatgctg ctcgacgagc
      901 ccaccgccag catggactcg gatgcgcgaa acaaagttga gacactcctc ggcgaattca
      961 ccagcgggcg atcccagacg ctcatcttca gcagccacac gcttggacag gtccggcggc
     1021 tcgcgagccg cgtgatctgc ctgcaggacg gccgcataca ggctgacctt ccaatacagg
     1081 aattcctgct gcgcaacgat gcgaccgagt cgggactttc aattgaaggg atactgaatt
     1141 gagatttatc gggcgagcga gagtcctctt cgcggcaatc ctgtgcattg cgagcggtat
     1201 ggcgggagcg cagagcatca cgctgtcgtc gaccacatcc accgagcagt cggggctgtt
     1261 tgcgcatctg ctgccgcagt tcaagcaggc gggcggcgtc gacgtgaagg tcgtggcggt
     1321 cggcaccgga caggcaatgg acatcgcgcg tcgcggcgat gccgatgccc tcctcgtgca
     1381 cgaccaggtt gcagaggaga aattcgtcgc cgagggatac gggctcaagc gccatccggt
     1441 catgtacaac gacttcgtgc tggtcgggcc gaagaccgat cctgccgcgg cacgcggctc
     1501 ggacattgtt gcggctctcg gcaaaatcag cgccggcaat gcagccttca tctcgcgcgg
     1561 tgacaagagc ggcacccatt ccgccgagag ccggtactgg actacggccg gtctggcgag
     1621 ccagcgcggc agcggctaca gggagtgtgg gtgcggcatg ggtccggcat tgaacatcgc
     1681 cgccgcttca ggagcctacg tcctgaccga tcgcggcacc tggctggcat tcaagaaccg
     1741 cggcgacctc accgtgctcg tcgaagctga caagcgcctg ttcaaccagt acggcgtgat
     1801 cgtggtgaat cccgcaaagc acccgcatgt aaaggtcgcg caggcacagc agtttgcgga
     1861 ctggatgatc tcgcgcgccg gacagtcggc gatcgccgcc tacaagatcg gtggagaaca
     1921 gctcttcttc ccgaacgcgg gcgagtagcg caggcaggac ctgcgggggc cgtgcaagga
     1981 tccctccaga cgctggagtg acggaagcac gaacgctgct tcttccgaac cgggcgattg
     2041 acctcgccgc gagcgcggga cgagttgcgc gacaatgtcc tgaatgaagg ctgccacccc
     2101 tacgatcgcc gacgtgcgaa tgcacggatt ccggaaacgc agcgaagtgt cggctgtgtt
     2161 cgactggatc gatcaggccg cgcgccggct gggcgcagag tcgcttgcgc tcgagcaggc
     2221 caccggccgc acgctggcgg ccgaagtgat tgcaccgctc gacgtcccgg gcttcgatcg
     2281 ggccgccatg gatggcttcg cactgcgcgg cgcggaaacg gccggagcca ccgactacaa
     2341 ccccctcgag tttcgcgtgc tgggccaggc catgcccggc cagccgttca ccggggaact
     2401 gctgccccgc gcggccgtgc gaatcatgac cggtgcgccc gtctctgcag gcgtcgacgc
     2461 ggtcgttccg gctgagtacg ccactgaaac cgacgggcag atcatcgtca ctcgacctgt
     2521 tgccccggga cagcacgtcg gccatcgtgg cgaggacctg cgggcaggat cgactgcgct
     2581 ggctgccggc cgccgcctgc gaccacagga tctgggactc gcggcgtccc tgggcctgga
     2641 ccacgtgacc gtcgtccggc agccccgtgt gcgcatgctc gtcactggca acgaagtgaa
     2701 ggcccccgga gcgccgaaag gcatgtacga ggtctacgac gcgaactcgt acatgctgcg
     2761 cggcctgatt gcccgcgacg gcggagtact cgaatcgcat ctccgcctgg gcgacgatcc
     2821 ggatgcgata cgcgcggccc tgctcgcgcc cggagccgat gtgatcctcg tgtccggcgg
     2881 ctccatcgtc ggccgcgagg actatgcgcc gcgcttgatc gccgagatcg gcgaactggc
     2941 aatacacggc gtggcgatgc gaccgtcgag tcccgccggc gtcggacgaa tcggcgagac
     3001 gctggtgttt ctgttgcccg gcaatcccgt gtcgtgcctg tgcgcctacg atttctttgc
     3061 aggtcgcgct ctgcgcctga tcggcggtcg atcgcctgac tggccacatc gaagtgtgca
     3121 ggctgttgtt gcgcgcaaga tcgtttccgc catcggacgc gtggactact gtcgcgtcca
     3181 gcttgtcgac ggccgagtcg aaccgatcgc gctgagtggc gcctcgatcc tttcgtcgac
     3241 aacccgggct gacggcttcg tcatcgtgcc gtcgaacagc gaaggctatg ggcctgacgc
     3301 ggtcgtcacg gcatacctct acgactgagt gcgccttcga acatgtcgac ttcacacgaa
     3361 tccggtgcgc aaggcgcgga gcctatcgcc gggcagtccc agttcctgaa cgtcgtttcg
     3421 cgggacgagg ccactgctcg ttttcgtgag cacctgcggc tcactccgct gggcgaggag
     3481 ttcgttgctc tcgcagaggc cctcggccgc gtgctcgcca gggatgtcgc ggcagacatc
     3541 gacgtgccag ggttcgatcg cgccaacgtc gacggctttg cggtgcaggc gcaggacacg
     3601 tttggcgcca tggaggaagc accgcgcacg ctgcgactca acgacgaagt gatcgcaccc
     3661 ggaatcgtgc ccaacctgcc ggtgacttgc ggccacgcaa cgatgatcgc cacgggcggc
     3721 atgcttccgc gcggcgccga cgccgtgctg atggtcgagc attcggaact tgtcggacac
     3781 gcagacactc ggaatatcga ggttcgccgg ccccttaccg ggggcgagaa cgtgagttac
     3841 gccagcaccg atgttgcgcg cggggagacg gtgctgcgtt ccggacaacg cctcacgtcc
     3901 cgcgagatcg gcgtactggc cgcactggga cgatcggagt gcccggtctt tcgtcggcct
     3961 cgagttgcca tcttttcgac cggcaacgaa atcgtggcac ccggcgattc cttgcgccca
     4021 ggtgcggtct acgactccaa tgccgcgatc atcggggctg ccgtcgagga actcggtggc
     4081 attgctgtct acctgggtgt gatcgccgac aacgagcggg cgctggcggc tgcgctggcg
     4141 cgcggcctgg agtcggacgt cgtcgtgttc tccggcggga cgtcgaaggg cgcaggagat
     4201 atctcgtacc ggatcgtgca cacgctgcgc aatcccggcg tcgtcgcgca cggtgttgcg
     4261 ctgaagcccg gcaaacccat ctgcctcgcg gtcaccgaag gcaagcccgt cgtcatcctg
     4321 cctggattcc cgacgtcggc catcttcacg ttccacgaat tcctcgcgcc ggtgatccgc
     4381 gcgtttgccg gcttgccgcc cgagcggcgc cacaccgtgc cggcgacgtt gccgatgcgc
     4441 gtgaactccg aacggggacg caccgagtac ctgctggtgg ggctggtgca gggacccgac
     4501 gggtatctgg cttatccgat gggcaagggc tcgggttcgg tcaccacctt cagcggcgcc
     4561 tatggcttca tcaccatcga ccagcacacc gagatcctcg atgccggaag tgcggtcgcg
     4621 gtgacactgc ttggcgagca gttggagccc gccgatctcg tgatcatcgg cagtcactgt
     4681 gtcggcctcg acctgctggt cggcaagctc acgcgccagg gcatccgggc caagccgatg
     4741 atcgtcggaa gccagggagg cctggccgcg gcccgtcgcg gtgagtgcga catcgccggc
     4801 atccacctga tggaccctgc cacaggcgaa tacaaccgac cgatgctcag cgacaccctg
     4861 accctcgtcg caggctacgg ccgcatgcag ggcttcgtgt tccggcgggg cgacgggcgc
     4921 ttcgagggca agtcgttcaa agaagcgatc agtgcagcac tgggcgaacc cggttgcgtg
     4981 atggtgaatc gcaacgccgg cagcggcact cgtatcctca tcgacaagtg gctggctcag
     5041 gagaggccca ctggatacgg tacgcaaacg aagtcccaca acgccgttgc cgtggccatc
     5101 gcacagcagc gggccgactg gggcatcgcg atcgatacgg tggcccgcca gtacggactc
     5161 gccttcatcg ccatgcagga agagcaatac gacttcgtcg taccgacgtc ccggctcgag
     5221 cgccccgcgg tgcaggcgtt cctcgcgctg ctggatgatc cggaagtgcg caatgcgctt
     5281 gcgtcgctgg gattccgctt accgcagaag tcgcaacgcc agcctgccgg ctcagcacgc
     5341 gtacccctga cggagagcga cgaatgattt cccggctctg tgaaacgggg agccgagcgc
     5401 tccgggtacg atggccaact tgggactcaa tacaatgaac gttgcgctga ctcacaccca
     5461 caccctcact cgcataggct tggccatcat tgcagtgctg ttgactgcct gtgcgtcagt
     5521 cggctccggc ccgggggcca cgggaggtcc gggaaaggcc ggcgcggtca cgccgccgag
     5581 cctgggcctg ttctccagta tcaaggtgcc ctccgatcgc gaaccgacac tgcaactctc
     5641 cgcgcagggg gtgcagatct tccgctgcga aaagcgcgat ggcgggtttg cctgggtgtt
     5701 ccgacagccg gaggcacagt tgtccgacgc gagcggcaag cccgttggcc ggcatggcgc
     5761 cagcttttcg ttcgagcaca acgacggcag ccggctcgtc gcgtccgttg ccgcgtacga
     5821 cgaggcgccg aatgccgccg acttgcgttg gctgctgctg acgactcgca gctacggcaa
     5881 gggtgctttc gacggcgtca cccacgtcca gcgcgtcaac acgcgcggcg gcatgcctcc
     5941 cgcgaaatgc gatgcagcgc gggcaaacca gcttctgcgc gtcgattttt cggctgattt
     6001 cgtcttctac gggatcaagt cgggagcggc cggctgaccg atcgcaatga ggatccgagg
     6061 cgtcgcaggt cgcgccccca gcccacgctc cgaggcattt ctcaaaaagc gttaaaattc
     6121 agcgttttcg gacgctgtac gccggaggaa tgcacatgaa cccgactgcc tcgacggtag
     6181 gcgcctacct gcgcgacctg ccgccggacc gtcgcgtggc aatggcgcgc ctatgcagca
     6241 tgattcgtcg ctccgcccgc ggagtgcgcg aaagcatgcg ttacgggctc gccttctacg
     6301 aactggacgg gccgctgttc gccctggaat ccggccgccg tcatatgtcg ctgtatgtgg
     6361 cggaaaaaga cgtcgtgctg aagcacaagg acgcgctgca gggcgtcaac gccaacaaga
     6421 gcttcatcaa attttcggac ctcaatcgac tgccgctcga tgtcgtcgag aagatcgtgc
     6481 gcgactcggt tgcggcgcgt cggcagcgat tggcgcaggg cacgataccg acgcagcccg
     6541 aactgctgaa gctctggggc atcaacgaag aagctgcagc gcctccggca acgacagtga
     6601 agctgcccgt cgcgaagccg gctgcctcga tgagcgatgc gatcaatgcc atggccagca
     6661 ccgtgcggga tgcgatgatc gcggcgatcg acatggccac gggcatgccg gtgactgaga
     6721 aacccaaggc gcgcaaggca ggcccgtcgc ggaccgcggc ggcgaaggca caccccgcga
     6781 agcgcaccac gtcccgtgcc gcaacgcgca cgacgtcgac gcgcaacccg ccgaaaaagg
     6841 cagccgccaa gaaggccgtt tcggcacgcc gccgctagtc tcttatgcct caccgagcgg
     6901 cgcctgcggg cgccgctcga gtttcaaggc tcagcgggcg gactcagtgc gccgattcag
     6961 tgcgaagact cagctcgcac gagtgtcctc gatgaccttg ccatcgttgg gcaaggctcc
     7021 cggggcaacc agttc
//
LOCUS       DATIRT010000013         3208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1083309, whole genome shotgun sequence.
ACCESSION   DATIRT010000013 DATIRT010000000
VERSION     DATIRT010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083309"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VLN25_00285"
     CDS             complement(<1..209)
                     /locus_tag="VLN25_00285"
                     /inference="COORDINATES: protein motif:HMM:NF013089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HSQ09045.1"
                     /translation="MYAGTRRHRTPPPEPTDRFINMSWMQPTPAQAWILLALAGLLEI
                     GWAIGMKYTDGFSRAVPSAIVMSAAL"
     gene            317..697
                     /locus_tag="VLN25_00290"
     CDS             317..697
                     /locus_tag="VLN25_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HSQ09046.1"
                     /translation="MERHFFAIGSLSALIGVAAGAFGAHALKARLPPELLIAFETGVR
                     YQLVHSLALLAVAWAVVRWPGRATTSAGVLFLAGIVLFSGSLYALSLTGARTLGIVTP
                     FGGVAFIAGWASLAWGAWRTQPRA"
     gene            728..1663
                     /locus_tag="VLN25_00295"
     CDS             728..1663
                     /locus_tag="VLN25_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HSQ09047.1"
                     /translation="MSHTARRLFLAGLALAIVGSVLFSAKAIVVKLAYRHGVDAATLI
                     GLRMAFSVPFFIAAYLWSSRGAAALTAQDHRRLIVIGLLGYYAASYLDFLGLQFITAA
                     LERLILYLNPTIVLLMSALFLGKRFSRRDVAALALAYGGILLVFLHDVSLEGEGVPLG
                     AVLVFGSAVCYAAYLVMSGELVRRVGAIRLTSYAMCVSTGAVFAQFALLNPLSALKQP
                     APVYWLSLVNGLLCTVLPVFATMMAVERIGAGRTSLAAMVGPVATIALAYVFLGETVG
                     GWQIAGTALVLSGVYVLSRPSTAPAPGGSASKETP"
     gene            1660..2640
                     /locus_tag="VLN25_00300"
     CDS             1660..2640
                     /locus_tag="VLN25_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HSQ09048.1"
                     /translation="MKAKSIVVRAHGGADVLELTELDVPDPGPDDVRIRQNAIGLNYI
                     DVYYRTGLYSGALPHGLGFEGAGVVEAVGSNVKFLKAGDRVAYPQGPLGAYSEARTMP
                     AATVVRLPKQISFEQGAAVMLKGLTVQYLFRQTYRLQGHETILFHAAAGGVGLIACQW
                     ARALGVKLIGTVSSPEKAALAKKAGAWQTIDYSKENFVERVQQLAGGRKLPVVYDGVG
                     KTTWEGSLDCIEPRGLMVSFGNASGAVTGVAIGILAQKGSLFLTRPTLATHIVPRARL
                     EASSEELFGLMISGKIRVDIEQRYPLAEAARAHRDLEARKTVGSTVLLPG"
     gene            2762..>3208
                     /locus_tag="VLN25_00305"
     CDS             2762..>3208
                     /locus_tag="VLN25_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HSQ09049.1"
                     /translation="MSSIPNQIAIVAAAWGAVVLTGCASMNAAPSAQAELKPTQGNTA
                     SGWVRFEQRGSGVLVTAEVRGLKANTEHGFHVHEKGDCSAPDATSAGGHFNPGGSPHA
                     HYLQGARHAGDMTNLMADASGVARYSYTSDMLSVAGGAANGVLGRAV"
BASE COUNT          520 a          999 c         1098 g          591 t
ORIGIN      
        1 agtgcggcgc tcatgacaat ggcactcgga accgcacggg agaagccgtc cgtgtacttc
       61 atgccgatgg cccagccaat ttcaagcagc ccggcgagtg cgagcagaat ccatgcttgg
      121 gcgggtgtcg gctgcatcca ggacatgttg ataaaccggt cggtgggttc aggtggcggc
      181 gtacggtgac ggcgtgtacc tgcgtacatg gtaaccactt gcgcccggtg tccctgcgca
      241 gatcagcttg cgaatgggag caaagttcgg acaggtcgac cgacaatcgg acgtaatccc
      301 cttaagaagg aactcaatgg aacgccactt ctttgcgatc ggatcgctgt cggcactgat
      361 cggagtggcg gcgggtgcgt ttggcgcgca tgcgctcaag gcacgactgc ctccggagct
      421 gctgatcgct ttcgagaccg gggtgaggta ccagctggtg cactccctcg cactgctggc
      481 ggtcgcgtgg gcggtcgtgc gctggccggg ccgcgcaacc acgtccgccg gagtgctgtt
      541 cctcgccggc atcgtcttat tcagcggcag cctgtacgcg ctctcgctga ccggggcacg
      601 cacgctgggg atcgtcaccc cgttcggcgg agtggcgttc atcgcgggct gggccagcct
      661 cgcctggggc gcatggcgta cacagccgcg cgcctgaacc gcacgggcgc ctccgtagaa
      721 tgtgcctgtg tctcacaccg cccgccgcct gttcctggcc ggactcgcgc tggccattgt
      781 cggcagcgtg ctgttctcag ccaaggcgat cgtcgtcaag ctcgcctacc ggcacggtgt
      841 cgatgccgcc acgctgattg gcctgcgcat ggccttttcg gtgccgtttt tcatcgccgc
      901 gtatctgtgg agcagtcgcg gtgcagcggc actgacggcg caggaccatc ggcgactcat
      961 cgtcatcggt ctgctcggat actatgccgc gagctatctc gacttcctcg ggttgcagtt
     1021 catcaccgca gcgctcgagc gcctgatcct gtacctcaat cccacgatcg tccttctgat
     1081 gtcggcattg tttctcggca aacgcttcag tcggcgcgac gtcgcggcac tcgcactcgc
     1141 ctatggcggc attctgctgg tattcctgca cgatgtcagc ctcgagggcg agggcgtccc
     1201 gctcggcgcc gtgctggtgt tcggaagcgc cgtctgctat gccgcgtatc tcgtgatgtc
     1261 gggagaactg gtgcggcgcg tcggcgcgat ccgcctgacg tcctatgcga tgtgtgtgtc
     1321 gacgggtgct gtattcgcgc agttcgcact gctcaatccg ctcagcgccc tgaagcagcc
     1381 ggcgccggtc tactggctgt cgctggtcaa tggactgctg tgcacggtgt tgccggtgtt
     1441 cgccacgatg atggcggtag agcgcatcgg cgcaggccgc acgtcgctcg cggcgatggt
     1501 gggaccggta gccacgatcg cactggccta tgtgtttctc ggtgagaccg tcggtggctg
     1561 gcaaatcgcc ggcaccgccc tcgtgctctc cggtgtgtac gtgttgtcca gaccatccac
     1621 agcgcccgcc cctgggggca gcgccagcaa ggagacccca tgaaagcgaa gtccattgtt
     1681 gtccgcgcgc acggcggtgc ggacgtgctc gaactcactg aactcgacgt gccggatcct
     1741 ggcccggacg acgtgcgcat ccgtcagaac gcgatcgggc tcaactacat tgacgtgtac
     1801 taccgcacgg ggctttacag cggcgcgttg ccgcacggcc tcggcttcga gggcgcgggt
     1861 gtggtcgagg ccgtcggcag caacgtcaaa ttcctgaagg caggcgaccg cgtggcgtat
     1921 ccgcagggtc cgctaggcgc ctattccgag gcgcggacga tgcctgcggc caccgtggtc
     1981 agactgccga agcagatctc gttcgagcag ggtgcggcgg tgatgctgaa gggcctgacg
     2041 gtgcagtacc tgtttcgcca gacctatcgc ctgcagggac atgaaacgat cctgttccac
     2101 gcggccgcgg gcggcgtcgg gctgattgcc tgccagtggg cgcgcgcgct tggagtcaag
     2161 ctgatcggca ccgtgagttc tccggagaag gcggcgctgg cgaagaaggc aggcgcctgg
     2221 cagacgatcg actacagcaa ggaaaacttc gtcgagcgtg tgcagcagct cgccggcggc
     2281 aggaagctgc cagtcgtcta tgacggcgtg ggcaagacca cctgggaggg gtcgctcgac
     2341 tgcatcgagc cgcgtggcct gatggtgagc ttcggcaacg cctcgggcgc cgtgaccggt
     2401 gttgccatcg gcatcctggc gcagaagggg tcgctcttcc tgacgcggcc gacgctcgcg
     2461 acccacatcg tgccgcgtgc gcggctggag gcctcgtcgg aggaactctt cggcctgatg
     2521 atctccggca agatccgggt cgacattgaa cagcgctatc cgctcgcgga ggcggcccgc
     2581 gcgcatcgcg atctcgaagc gcgcaagaca gtggggtcga cggtactgtt gcccgggtag
     2641 cgtatccgct gcaaatcggc cgaccgcgca agcggcgaag ccggtgctag cctagccaag
     2701 cggcggctta gcgcgcaggc gcactccgct ggacaatgtg atatcgaaaa ggagacccat
     2761 catgagctcg attccgaatc aaatcgccat cgtcgcagcg gcatggggtg ccgtcgtctt
     2821 gaccggctgt gcgagcatga atgcggcccc ctcggcacag gcggaactga agccgacgca
     2881 gggcaacacc gcgagcggct gggtgcgctt cgagcagcgc ggcagcggcg tgctcgtgac
     2941 cgcggaggtg cgcgggctga aggcgaacac cgaacacggt ttccacgtgc atgagaaggg
     3001 cgattgttct gcgcccgacg cgaccagtgc cggtggacat ttcaatccgg gtggcagccc
     3061 gcatgcgcat tacttgcagg gtgcgaggca tgccggcgac atgacgaacc tgatggcaga
     3121 tgcgagcggt gtcgccaggt acagctacac cagcgacatg ctgagcgttg caggcggcgc
     3181 ggccaacggt gtgctcggac gtgccgtg
//
LOCUS       DATIRT010000014         2358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1902, whole genome shotgun sequence.
ACCESSION   DATIRT010000014 DATIRT010000000
VERSION     DATIRT010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1902"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VLN25_00310"
     CDS             <1..778
                     /locus_tag="VLN25_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09050.1"
                     /translation="RFGGKVIVAAMATFGNPGEFAIALSNGEFSTLRFGVPQPTASPA
                     GGAERGRVIPTADSVYANVLVRVGQTSDFTAGLTETWYSGLALLRTDGSELLFVDPSY
                     SVASPSRYFDVAQFTGVSRTIDLCYIGISASGALIDDPLRAFEIVRQARGPECSAIAP
                     NGPITPRINRIAYDDPRSVFNGCRRHVTLGATRITNGGGSTLWYSDPYGRATRTASFT
                     GGVKQFIGVINNSTAGAVDRVAFGADLDPCLSGSNIHAPN"
     gene            851..1936
                     /locus_tag="VLN25_00315"
     CDS             851..1936
                     /locus_tag="VLN25_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006053016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ionic transporter y4hA"
                     /protein_id="HSQ09051.1"
                     /translation="MNRAFFGVTAWAALIPWVAVGALIAAWGRPLTSVTISLVAAVLV
                     AAVFAAVHHAERIALRIGEPFGTLVLALAVTVIEVALIASMMLGGGPGASTLARDTVF
                     ATVMIVCNGVVGLCLLVGGLKHGIAEFRVEGASQALSVLIPLAMLTLLLPNYTTTTRG
                     PTYSAGQIEFIGAMSLVLYVAFVFVQTVRHRDYFLSVGADGAEDHGTPPSTAGALASL
                     ALLFLCLVAVVGLAKALAPTIENTVAGAGAPFAVVGIVIALLVLLPETLSAVRAARAN
                     RMQTSLNLTLGSALATIGLTVPTIGALSVVLDLPLILGLPATESMLLATTLLVSTVTL
                     ATGRSTLLHGAVHLSLFGAFLFLAVVP"
     gene            2036..>2358
                     /locus_tag="VLN25_00320"
     CDS             2036..>2358
                     /locus_tag="VLN25_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09052.1"
                     /translation="MLLFASTILLSAFLLFLVQPIIAKQILPWFGGTSAVWITCLVFF
                     QVVLLAGYTYAHVITRRLSPRRQSQLHIALLLASLLFLPIVPSAALKPAADADAALRI
                     IVLLAA"
BASE COUNT          325 a          811 c          763 g          459 t
ORIGIN      
        1 gcgcttcggt ggcaaggtca tcgtggccgc catggcgacg ttcggcaatc caggtgagtt
       61 cgccattgca ctgtcgaacg gggagttctc gaccctgcgg ttcggtgtgc cgcagccgac
      121 agcgtcgcct gccggcggcg ccgagcgcgg gcgcgtaatc cccacggccg attccgtgta
      181 tgcgaatgtg ctggtgcgtg tcggccagac gtcagacttt acggcgggcc tgaccgagac
      241 gtggtattcg ggtctggcac tgttgcgcac cgatggcagc gagcttttgt tcgtcgaccc
      301 gtcctattcc gtggcatcgc cgtcacgcta cttcgatgtg gctcagttca ctggcgtgtc
      361 gcgcaccatc gatctctgct acatcggaat cagcgcgtcc ggtgcactga tcgatgaccc
      421 gctgcgggcc ttcgagatcg tgcgccaggc gcgcgggccc gagtgctccg ccatcgcacc
      481 caatgggccg atcacaccgc gcatcaaccg gattgcctac gatgatcccc gcagcgtgtt
      541 caacggctgt cgtcgtcacg tcacgctcgg cgcaacccgc atcaccaatg gcggcggctc
      601 gaccctctgg tacagcgatc cgtacggccg cgcaacgcgc actgccagct tcaccggcgg
      661 ggtcaagcag ttcatcggcg tcatcaacaa cagcaccgcc ggcgccgtcg accgcgtggc
      721 cttcggcgcc gatctcgatc cctgcctgtc gggcagcaac attcatgcgc cgaactgaag
      781 cagcggcagc gcgctcgctg cgcgcggatc acggctggaa cgtacggacg aagtcttcga
      841 gccgggcccg atgaatcggg ctttcttcgg cgtgacggcc tgggctgcgc tcataccctg
      901 ggtggcggtc ggtgcgctca ttgcggcgtg gggacggccg ctgacctccg taacgatcag
      961 tctggtggcg gctgtactgg tggcggcagt gttcgcggcg gtccatcacg ccgaaaggat
     1021 cgcgttgcgc atcggagagc cgttcggcac gctggtgctg gcgctcgccg tgacggtcat
     1081 cgaagtcgcg ctgatcgcgt cgatgatgct cggcggcggt cctggcgcca gcacgctcgc
     1141 ccgcgacacc gtattcgcga cggtgatgat cgtctgcaat ggcgtggtcg gactgtgcct
     1201 gcttgtcggc ggtctgaaac acggcatcgc ggaatttcgg gtcgaagggg cgagtcaggc
     1261 cctgtcggtg ctgattccac tcgcgatgct gacgctgctg ctgcccaact acacgacgac
     1321 gacgcgcggc ccgacctact cggctggcca gatcgagttc atcggcgcga tgtcgctggt
     1381 cctttacgtt gcgttcgtct tcgtgcagac cgtgcgccac cgcgactact tcctcagcgt
     1441 cggagccgac ggcgccgaag accatggcac gccaccttcc accgccggcg cgcttgccag
     1501 cctggcgctg ctgttcttat gcctggtcgc cgtcgtcggg ttggccaagg cgctggctcc
     1561 gaccatagaa aacacggtcg ccggtgccgg cgcaccgttc gcggtcgttg gcatcgtgat
     1621 cgcgctgctg gtgctgctgc cggagaccct gtcggctgtg cgcgccgcgc gcgcaaaccg
     1681 catgcagacc agcctcaacc tgacattggg atcggcgctc gcgacaatcg gcctgacggt
     1741 gccaaccatc ggtgcgctct cggtggttct cgatctgccg ctgatcctcg gcctgcccgc
     1801 gaccgaatcc atgctgctcg ccaccacgct gctcgtctcg acggtgacgc tggcgaccgg
     1861 ccgctcgacg ctgctgcacg gcgcggtcca tctgtcgctg ttcggcgcgt tcctcttcct
     1921 ggcggtcgtg ccctgaacgg cggcgggcga agggcgcccg catgagagaa tcgcggccct
     1981 ttggctgcgc acggcgccaa ggccgcacgg cgcacgcgtc cattcctcct gacccatgct
     2041 gctcttcgct tccacgatcc tgctgtcggc gttcctgctg ttcctggtgc agccgatcat
     2101 cgccaagcag atcctgccct ggttcggcgg cacctccgcg gtgtggatca cgtgcctggt
     2161 gtttttccag gtcgtgctgc tggccggcta cacctacgcg catgtgatca cgcgacgact
     2221 ctcgccgcgc cggcaatcgc aattgcatat cgcgctgctg ctcgcgagcc tgctgttcct
     2281 gcccatcgtg cccagcgcag cgctgaagcc cgcggccgat gctgacgccg cgctgcgcat
     2341 catcgtgctg ctggccgc
//
LOCUS       DATIRT010000015         2292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_788575, whole genome shotgun sequence.
ACCESSION   DATIRT010000015 DATIRT010000000
VERSION     DATIRT010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788575"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..2097
                     /gene="hyfB"
                     /locus_tag="VLN25_00325"
     CDS             79..2097
                     /gene="hyfB"
                     /locus_tag="VLN25_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HSQ09053.1"
                     /translation="MTAPADWLAIDWLLAAITSWIAIGAAGMVRPHGFYVVARILFPL
                     GALISVTVAVCAAIAVPGAAETAVLPLGLPGLPFHLRLDSLSAFFLLVLGSASAGVSA
                     FASGYFRKGEGTPPGLLCLLYHLFLASMAMVLLADDAYVFMVMWELMALTSFFLVTAN
                     HVIPEIRRAGYLYLLIAHIGALAILLCFGVLQANTGDYTFANMRAQELTPFWASVAFL
                     LAFFGFGAKAGIVPLHIWLPEAHPAAPSPVSALMSGVMLKTAIYGLLRIGFEVLQTQI
                     WWWGVLALAIGLVTALFGVVFAAIQTDMKRLLAYSSIENIGLLVVGIGLALLFVSYGM
                     APMAALALTAVLYHALNHTFFKSLLFLGTGAVLHATGERNLGKLGGLLRFMPWVGWLS
                     LAGALAAAGLPPLNSFVSEWLLLQSFLFTLGLKSTFVNNLVPVVAAGIALVAAIGGYV
                     MVKFFGVIFLGQPREARLAASQDAVSWERAGLLWLAIGCVALGLFPAQVIALIDPVTR
                     TLVDDGIGPQVAAHGWLILAPVDPNRASYSPLLFLLLMATSFALAFVLVRWLYHGRVR
                     RAAPWDCGYPLQTPRMQDTAEGFGQPIRQVFEPFFRIERHLPTPFDKQPYYHLAAGDH
                     FWHWLYLPVARLTEATSRLVGRLQQGRISTYLMYSFVTLLVMLVLVFR"
     gene            2094..>2292
                     /locus_tag="VLN25_00330"
     CDS             2094..>2292
                     /locus_tag="VLN25_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase"
                     /protein_id="HSQ09054.1"
                     /translation="MTVYSVMAQVLAILLAVLGSPLLTGWVNQCRAWLQNKSAPPLLL
                     PYRTLHKLFWKESVVAENASPL"
BASE COUNT          301 a          769 c          731 g          491 t
ORIGIN      
        1 ctcaggcagc cgccctctgc cccagtggct gatcgtcgcg tccaagcagg cgggcaaccg
       61 gcgccagaga ctcaagccgt gactgccccg gccgattggc tcgccattga ctggcttctg
      121 gccgcgatca cttcgtggat cgccatcggt gcggccggca tggtgcggcc gcacggcttc
      181 tacgtcgtag cgcgcatcct gtttccgctg ggcgcactga tcagcgtcac cgttgccgtc
      241 tgcgccgcga tcgcggtgcc cggagcggcc gagaccgctg tgctgccgct gggcctgccg
      301 ggcctgccgt tccaccttcg actcgactcg ctgtcggctt tcttcctgct ggtgctcggc
      361 agcgccagcg ccggcgtctc ggcgtttgct tccggctact tccgcaaggg cgaaggcacg
      421 cctccagggt tgctgtgcct tctctatcac ctgttcctcg ccagcatggc gatggtgctg
      481 ctggccgatg acgcttatgt cttcatggtg atgtgggagc tgatggcgct gacgtcgttc
      541 ttcctggtga cggccaacca tgtcattcct gaaatccgcc gcgctggcta cctgtacctg
      601 ctgatcgcgc acatcggagc gctggcgatc ctgctctgct tcggagtgct gcaagcgaac
      661 accggggact acaccttcgc caatatgcgc gcccaggagc tgacgccgtt ctgggcctcg
      721 gtcgcattcc tgctggcgtt cttcggcttt ggtgccaagg cgggcatcgt gccgctgcac
      781 atctggctgc ccgaagcgca cccggcggcg ccctcgccag tgtctgccct gatgagcggt
      841 gtcatgctga aaactgccat ctatggcctg ctgcgcatcg gcttcgaagt gctgcagacc
      901 cagatctggt ggtggggcgt gctggcgctg gcgatcggac tggtaacagc gctcttcggc
      961 gtggtgttcg cggcgattca gacggacatg aaacgcctgc tggcctactc gtcgatcgag
     1021 aacatcgggc tgctcgtcgt cggcatcggc ttagcgctgc tcttcgtcag ttacggcatg
     1081 gcaccgatgg cggccctcgc cttgaccgct gtgctttacc acgcgctgaa ccatacgttc
     1141 ttcaagagcc tgctcttcct aggcaccggc gccgtcctgc acgcgacggg agagcggaac
     1201 ctcggcaagc tcggcggcct gttgcgcttc atgccgtggg tcgggtggct ctcgctcgca
     1261 ggcgcgctgg ccgcggccgg cctgccaccg cttaacagct tcgtgtccga gtggttactg
     1321 ctgcagagtt tcctcttcac tcttggcctg aagagcacgt tcgtcaataa cctcgtgcct
     1381 gtcgtcgcgg cgggtatcgc actggtcgcc gcgatcggcg gctatgtgat ggtgaagttc
     1441 ttcggcgtga tctttctcgg ccagccgcgc gaggcgcggc tcgcggcttc gcaggacgcg
     1501 gtctcgtggg aacgtgcagg actcctgtgg ctggcgatcg gctgtgtcgc gctcggcctc
     1561 tttcccgctc aggtcattgc gctgatcgac ccggtcacac ggactctggt cgacgacggc
     1621 atcggccctc aggtggcggc gcacgggtgg ctcatcctgg ctccggtcga cccgaaccgc
     1681 gccagctaca gcccgttgct gttcctgctg ctgatggcga cgagcttcgc gctcgcgttc
     1741 gtgctcgtac gctggctcta ccacggtcgc gtgcgacgcg ccgcgccgtg ggactgtggc
     1801 tatccgctgc aaactccgcg catgcaggac accgccgagg gcttcgggca gccgatccgc
     1861 caggtgttcg aacccttctt ccgaattgag cggcacctgc cgactccctt cgacaagcag
     1921 ccctattacc acctcgccgc gggcgatcac ttctggcatt ggctgtactt gccggtggcg
     1981 cggctgaccg aggcgacctc tcgcctggtc ggaaggctgc agcaaggccg gatctcgacc
     2041 tacctgatgt acagcttcgt cacgctgctg gtcatgctgg tgctggtgtt tcgatgaccg
     2101 tctacagcgt catggcccag gtgctcgcga tcctgctcgc tgtgctcggc tcgcccttgc
     2161 tcaccggatg ggtcaaccag tgccgtgcct ggctgcagaa caagagcgct ccgccgttgc
     2221 tgctgccgta ccgcacgctg cacaagctgt tctggaagga gtcggtcgtc gccgagaacg
     2281 cgagcccgct gt
//
LOCUS       DATIRT010000016         1752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_788637, whole genome shotgun sequence.
ACCESSION   DATIRT010000016 DATIRT010000000
VERSION     DATIRT010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788637"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="VLN25_00335"
     CDS             complement(<1..1322)
                     /locus_tag="VLN25_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HSQ09055.1"
                     /translation="MSAVRVTPSLFKAYDIRGVVDTALTPDAVRLIGSALGSEAAALG
                     ESAICVGRDGRLSGPMLRDALVAGITSTGTDVIDVGMVPTPVLYFSTVHLGTGSGVEI
                     TGSHNPPEYNGLKMMMAGTTLHGDAIQALRSRIESGNLVRAGTPGEERMADVLPAYLE
                     RIRTTVKLARPMKIAIDCGNGVAGPTAVTLFRTLGCDVVELFTDIDGTFPNHHPDPAH
                     PENLEDLIRALRDTDAELGLAFDGDGDRLGVVTKDGQIIYPDRQIMLFAQDILAAYPG
                     GEIIFDVKCTRRLAPWIREHGGRPTMWRTGHSLIKAKLKDSGAPFAGEMSGHLFFNDR
                     WPGFDDGLYAGARLLEILSRSADPSAVLNALPSAVSTPELHLHTAEGENFALVERLRN
                     QAKFEGAEQIITIDGVRAEWSDVFGLARPSNTTPVVVLRFEGDTPEALA"
     gene            complement(1367..>1752)
                     /locus_tag="VLN25_00340"
     CDS             complement(1367..>1752)
                     /locus_tag="VLN25_00340"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HSQ09056.1"
                     /translation="VPKLVAHFAQRLPHVELGNLDVVRDFSDVRTVAQAYVRLLTADV
                     PEGVTNLCSGVGRSLRWVVEQLREISGHALDVRVNPEFVRASEVHRLVGSSSRMESAL
                     GSLPFRDFRETLAWMLQASTAEARP"
BASE COUNT          341 a          584 c          534 g          293 t
ORIGIN      
        1 gccagcgcct cgggagtgtc cccttcgaac cgaagcacga cgaccggagt tgtgttggac
       61 ggacgcgcca gcccgaatac atccgaccat tcggcgcgca caccatcgat ggtgatgatc
      121 tgctctgcgc cttcgaactt cgcctggttt ctgaggcgtt cgaccagcgc gaaattctcg
      181 ccctccgcgg tatggaggtg cagttccggc gtcgacaccg cactgggcaa tgcattcagt
      241 acagccgacg gatcggccga ccgcgaaagg atctcgagca ggcgagcccc cgcatacagc
      301 ccgtcgtcga agccaggcca gcggtcgttg aagaagaggt ggccgctcat ctcgccggcg
      361 aatggcgcgc cgctgtcctt gagcttggcc ttgatcagcg aatgcccggt gcgccacatc
      421 gtcgggcgac cgccgtgttc acgaatccac ggagccagcc gtcgcgtgca cttcacgtcg
      481 aagatgatct cgccgccggg atacgccgcc agaatgtcct gcgcgaacag catgatctgg
      541 cggtccggat agatgatctg cccgtccttg gtcacgacac cgaggcggtc gccgtcacca
      601 tcgaaggcca gccccagttc ggcatccgta tcccgcagcg cgcggatcag gtcctcgaga
      661 ttctcgggat gcgccgggtc cggatgatgg ttcgggaagg tgccgtcgat atcagtaaag
      721 agttcaacca cgtcgcaacc caacgttcga aacagggtga cggccgtcgg tccggccacc
      781 ccgttgccgc aatcgatcgc gatcttcatc ggacgtgcca gcttgactgt ggtgcgtatg
      841 cgctcgagat acgcaggcaa cacgtccgcc atgcgctcct cgcccggcgt cccggcgcga
      901 acaagattgc cgctctcgat gcggctgcgc aacgcctgga tcgcatcacc atgcagcgtc
      961 gtgccggcca tcatcatctt caggccgttg tactcgggcg gattgtggct gccggtgatc
     1021 tccacaccgg aacccgtccc cagatgcacc gtggagaagt acagcacagg ggtcggcacc
     1081 atgccgacat cgatgacgtc ggtgccggtc gaggtgattc cggccaccag cgcatcacgc
     1141 agcatcggcc cggacagccg tccgtcgcgg ccgacacaga tcgccgactc gccgagcgcg
     1201 gcggcttccg agcccagcgc ggagccgatc agacgcaccg catccggcgt cagtgccgtg
     1261 tcaacgactc ctcgaatgtc ataggccttg aacagcgatg gggtaacgcg gactgcggac
     1321 atgggcacct acggttgaac tgcgcacgaa gccgaaacag tcagcatcat ggcctcgctt
     1381 cggcagtcga cgcctgcaac atccaggcaa gggtttcgcg aaaatcccga aacggcagac
     1441 tgccgagcgc agactccatt ctgctggaac ttccgacaag ccgatgcact tcactcgctc
     1501 ggacaaattc cggattgacg cgaacgtcga gcgcgtggcc ggaaatctcg cgcaactgct
     1561 cgaccaccca gcgcagcgat cgacccacgc ctgagcacag gtttgtcacg ccctccggga
     1621 catccgccgt cagcaggcgc acataggcct gggccacggt tcggacgtcg gaaaaatcgc
     1681 gcacgacatc gagattgccg agttcaacgt gcggcagccg ttgcgcgaag tgggccacca
     1741 gcttgggtac ga
//
LOCUS       DATIRT010000017         4008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_690582, whole genome shotgun sequence.
ACCESSION   DATIRT010000017 DATIRT010000000
VERSION     DATIRT010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690582"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VLN25_00345"
     CDS             <1..245
                     /locus_tag="VLN25_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-butyrobetaine hydroxylase-like
                     domain-containing protein"
                     /protein_id="HSQ09057.1"
                     /translation="QEGKRDVTINELEPVGHYAIKPTFSDGHDSGIFAWDYLYELGMN
                     QEQLWSDYLERLEAAGASRDPSAAPVTAPGRSCGHH"
     gene            273..1016
                     /gene="ubiE"
                     /locus_tag="VLN25_00350"
     CDS             273..1016
                     /gene="ubiE"
                     /locus_tag="VLN25_00350"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001240.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HSQ09058.1"
                     /translation="MSDHDRKADFGFEQVAEKEKAARVAGVFDSVASRYDLMNDVMSA
                     GLHRVWKAFTVRHANVRPGMKVLDIAGGTGDLAQAFARRAGESGEVWLTDINGSMLAE
                     GRDRLLDAGHTVPLAQCDAEKLPFPNTSFDRVSVAFGLRNMTHKDQALAEMARVLRPG
                     GRLLVLEFSRIWQPLGPLYDAYSFNVLPWLGKKLAGDSDSYRYLAESIRMHPPQDELA
                     RMMERAGFDDVQWFNLTAGVCALHVGTRY"
     gene            1030..1998
                     /locus_tag="VLN25_00355"
     CDS             1030..1998
                     /locus_tag="VLN25_00355"
                     /inference="COORDINATES: protein motif:HMM:NF016190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HSQ09059.1"
                     /translation="MNKWMVGIIIGTMAATFALDAEAQRRFGGGGNLGRQSPQVQQRQ
                     ATPPAQQPQAAPAQSGAQQAAPTKAPTAGAPVAARPAASPMRGMLMGLAAGLGIAALA
                     SWLGFGEALTMFLTVLLIGFALMMLVGFVMRRMRGPQPAVSAAGSGRSPGTYSNVGYE
                     TSPAPRPVPVQRAATPPMSGARPGSAMEQFMRTGAATTGWSIPPGFDTAAFLTHAKEH
                     FARLQRAWDVHNLDEISDFTTSDMFIALTHELRSRTGHSSTEVVALDAELLGIESNAD
                     EHIASVKFDGTLRIDGETESVSEVWNLVKPARGQGGWLLGGIQQMA"
     gene            2113..2694
                     /locus_tag="VLN25_00360"
     CDS             2113..2694
                     /locus_tag="VLN25_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HSQ09060.1"
                     /translation="MIEQLTTAAAVSVLNRMLARESWARDRLAPFAGRTARFEAPPFA
                     LALKVAEGGEFADAQRAEPVVTVGLDLAALPLALGDPQAALRNVSLKGDAEFAQALAF
                     VLQNLRPEPEEELSRFIGDAAAQRIVGLLRLTASHWRQLAERMLDSSAHYLVTENPML
                     VGRAEVDEFNAAVASLRDDVARLEKRVELHERA"
     gene            2749..>4008
                     /gene="ubiB"
                     /locus_tag="VLN25_00365"
     CDS             2749..>4008
                     /gene="ubiB"
                     /locus_tag="VLN25_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HSQ09061.1"
                     /translation="MRLLRLLRIFTVVWRYRLDEIVVSSLKNPTLVRAVRWTTFGARR
                     ETRGARLRQALEDLGPIFVKFGQVLSTRRDLLPTDVADELARLQDRVPPFASDVARRE
                     IERGLGRPIDSLFSTFEDEPVASASIAQVHFALMKEGPHAGKEVAVKVLRPNMLSAID
                     KDLSLLETGATLVEKLWRDGKRLRPRDVVREFDTYLHDELDLNREAANASQLRRNFAG
                     SPLLVVPEMYFDYCADNVLVMERMHGIPVSQIDRLNEAGIDLKKLSRDGVTLFFTQVF
                     DHAFFHADMHPGNILVGINGDQFNKYIGLDYGIVGTLTETDKNYLAQNLLAFFRRDYK
                     RIAELHVESGWVPEGTRINELEGGVRAVLEPVFDKPLKDISLGMVLLRLFQASRRFNV
                     EIQPQLVLLQKTLLNVEGLGRQLDPELD"
BASE COUNT          672 a         1285 c         1356 g          695 t
ORIGIN      
        1 tgcaggaagg caagcgcgac gtgacgatca atgagctgga gccggttgga cactatgcga
       61 tcaagccgac gttttccgac ggacacgaca gtggcatctt cgcctgggat tatctgtacg
      121 aactcggcat gaaccaggaa cagctctggt cggactacct tgagcgactg gaggcggccg
      181 gggcgagccg cgatccctcg gctgcaccgg tgacagctcc cggccggagt tgtggccatc
      241 actaaggcgt cgagcccagc ccgcgtgccg caatgagcga ccacgaccgc aaggcggact
      301 tcggcttcga gcaggtggct gagaaggaaa aggccgcgcg cgtcgcaggt gtattcgatt
      361 cggtcgccag ccgctacgac ctgatgaacg acgtgatgtc ggccgggttg catcgagtct
      421 ggaaggcgtt tacggtgcgg cacgcgaatg tgcgcccggg catgaaggtg ctagacatcg
      481 ccggtggcac gggcgatctg gcgcaggcct tcgcccggcg ggccggcgag tcgggcgagg
      541 tctggctcac cgacatcaac ggctcgatgc tggccgaagg ccgcgaccgc ctgctcgatg
      601 ccgggcacac ggtgccactg gcccagtgcg atgccgagaa actgcccttc ccgaatacct
      661 cctttgatcg cgtgtccgtg gccttcggac tgcgcaatat gacgcataag gatcaggcgc
      721 tggccgagat ggcgcgtgtg ctgcggccgg gcggtcggct gctggtgctg gagttttccc
      781 gcatttggca gccgctcggc cctctgtacg atgcgtactc gttcaacgtg ctgccgtggc
      841 tcggcaagaa actcgcgggc gacagcgaca gttaccgcta ccttgcggaa tcgatccgca
      901 tgcacccgcc acaggacgaa cttgcgcgaa tgatggaacg cgcgggcttc gacgacgtcc
      961 aatggttcaa cctgactgcc ggcgtatgtg cgctgcatgt cgggacacgc tactgattct
     1021 ggagatcgca tgaacaagtg gatggtagga atcatcatcg gcacgatggc cgcgactttc
     1081 gcgctcgatg ccgaggcgca gcgccgcttt ggcggcggcg ggaaccttgg ccgccagtcg
     1141 ccgcaggtgc agcagcgcca ggccacgccg ccggctcaac agcctcaggc ggcacccgcg
     1201 caaagcggtg cgcagcaggc cgcgccgacc aaggccccga cggcgggcgc tcccgtggct
     1261 gcgcggccgg cggcaagtcc gatgcgcggc atgttgatgg gccttgccgc cggtcttggc
     1321 atcgcggctc tggcctcctg gctcggcttt ggcgaggcgc ttacgatgtt cctgacggtg
     1381 ctgctgatcg ggttcgcgct gatgatgttg gtcggcttcg tgatgcggcg gatgcgcggt
     1441 ccgcagcccg cggtatcggc tgcaggatcg ggtcggagcc ccggcacgta ttccaacgtg
     1501 ggatatgaaa cgtccccggc tccgcggccg gtgccggtac agcgcgcggc cacgccaccg
     1561 atgtcgggcg ctcgtcctgg gtcggcgatg gaacagttca tgcggacggg cgcagccacg
     1621 acaggctgga gcatcccgcc aggattcgac acggctgcct tcctgacgca cgcgaaggag
     1681 catttcgccc gcctgcagcg ggcctgggac gtacacaatc tcgacgagat ctcggatttc
     1741 acgacgagcg acatgttcat tgcgctgacg cacgagctgc gttcccgcac tggacactcg
     1801 agcaccgaag tggtcgcgct cgacgccgaa ctgctgggca tcgagtcgaa cgccgacgag
     1861 cacatcgcga gcgtgaagtt cgatggcacc ctgcggatcg atggggagac cgagtccgtt
     1921 tccgaagtgt ggaacctcgt gaagccggcc cgtggtcagg gcggctggct acttggggga
     1981 atccagcaga tggcttgatc agcagatggc ttgacctccg cagcctgctc tcgggcagaa
     2041 gaggtggtaa ccaggggcgc gcttgcgccc ctttcctttt ccgggaccgg ttcggctgcg
     2101 taaactcgct gcatgatcga acaactgacg actgccgccg ctgtcagcgt gctgaaccga
     2161 atgctcgcgc gcgaatcctg ggcgcgcgat cggctcgcgc cgtttgcggg gcgcacggcg
     2221 cgcttcgagg cgccgccgtt tgcactcgcg ctgaaggtcg ccgaaggcgg tgaattcgcc
     2281 gatgcgcagc gcgccgagcc tgtggtgacg gttgggctcg acctcgctgc gctgccgctg
     2341 gcgctcggcg atcctcaggc ggcgctgcgc aacgtttcac tcaagggcga tgcggaattc
     2401 gctcaggcgc tcgcgttcgt gctgcaaaac ctgcggcccg agccggaaga ggagctatcc
     2461 cgcttcatcg gtgatgcggc cgcccagcgc atcgtcgggc tgctgcggct gaccgcgtcg
     2521 cactggcgcc agctggccga gcggatgctc gattcgagcg cccattacct cgtgaccgaa
     2581 aacccgatgc tcgtcgggcg cgctgaggtc gacgagttca atgcggccgt cgcgagcctg
     2641 cgcgacgatg tcgcgcgact ggagaagcgc gtcgagctgc acgagcgcgc ctagctcggg
     2701 tacccgcctg tacgggagcg cgtagcagcg gctcgtacaa tcgccgccat gcgcctgttg
     2761 cgtctgcttc gcattttcac ggtcgtctgg cgctatcgcc tcgacgaaat cgtcgtttcc
     2821 agcctgaaga atccgacgct cgtacgcgcc gtccgctgga ccacgttcgg cgcccggcgc
     2881 gaaacgcgcg gcgcgcggct acggcaggcg ctggaggacc tcgggccgat cttcgtgaag
     2941 ttcggccagg tgctgtcaac gcggcgtgac ctgctgccga ccgatgtcgc ggacgaactg
     3001 gcccggctgc aggatcgcgt cccgccgttt gcgtccgacg tggcgcggcg cgaaatcgag
     3061 cgtggactcg ggcgtcccat cgacagcctg ttctccactt tcgaggacga gccggtcgcc
     3121 tcggcttcga tcgcgcaggt gcacttcgca ctgatgaagg agggccctca cgccggcaag
     3181 gaggtcgcgg tcaaggtgct gcggccgaac atgctgtccg cgatcgacaa ggatctttcg
     3241 ctgctcgaaa cgggcgccac gctggtcgaa aagctctggc gcgacggcaa gcgcctgcgc
     3301 ccgcgcgacg tcgtgcgcga gttcgatacc tacctgcacg acgaactcga cctgaaccgc
     3361 gaggcggcca acgcgtcgca gttgcgtcgc aatttcgcgg gctcgccgct gctggtcgtg
     3421 cccgagatgt acttcgacta ctgcgccgac aacgtgctgg tgatggagcg catgcacggc
     3481 atcccggtga gccagatcga ccggctgaac gaggccggca tcgacctgaa gaagctgtcg
     3541 cgcgacggcg tgacgctctt cttcacgcag gtcttcgacc acgcgttctt ccacgccgac
     3601 atgcacccgg gcaacattct cgtcggcatc aacggcgacc agttcaacaa gtacatcggg
     3661 ctcgactacg gcatcgtcgg cacgctgacc gagaccgaca agaactacct ggcgcagaac
     3721 ttactcgcct tcttccggcg cgattacaag cgcatcgcgg aactgcacgt cgaatccggc
     3781 tgggtgccgg agggcacgcg catcaatgaa ctggaaggcg gggtgcgcgc cgtcctcgaa
     3841 ccggtgtttg ataagccgct gaaggacatt tcgctcggca tggtgctgct gcgccttttc
     3901 caggcgtcgc gccggttcaa cgtcgagatc cagccgcaac tcgtgctgct gcaaaagaca
     3961 ctgctcaacg tggagggcct cgggcggcag ctcgacccgg aactggac
//
LOCUS       DATIRT010000018         5083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_297380, whole genome shotgun sequence.
ACCESSION   DATIRT010000018 DATIRT010000000
VERSION     DATIRT010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5083
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297380"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1980)
                     /locus_tag="VLN25_00370"
     CDS             complement(<1..1980)
                     /locus_tag="VLN25_00370"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HSQ09062.1"
                     /translation="MALAVTGLLAALAWQLTRSPSPSAPIPAASPPSAALFVGSDECK
                     ACHAQETAAWTSSQHARAMQHATAETVRGDFNDAKFTFDGVTSSFFGRDGKYFARTDG
                     PDGKLAEFEVKYTFGLEQLQQYLVELPGGRLQALAVSWDARPKEQGGQRWFRQYPNEK
                     LDFRDELHWTQRAQNWNFMCADCHSTQVVKGYDANKNAFDTRFNEISVGCEACHGPGS
                     AHVAWTEAKPADASKGLTILLDERRGVTWSIDPATGNARRSRERTSEREIEVCAQCHA
                     RRAQIAEGYRAGQPFMDHYLPSLLLPGLYHVDGQQRDEVFIWGSWLQSRMHRQGVTCS
                     DCHDPHSQKLRVDGNAVCGQCHAPGKFDTPAHHHHAADSVTSAGAQCADCHMPRTLYM
                     VVDGRRDHSMRVPRPDQSVAFGVPNACNQCHTKQDAKWAAAAVRGWLGRDARGFQVFA
                     PSFRAAEVGQAGATASLATIANDLAHAPIVRASAVERLAQAGSVDALTARSAARDAQP
                     LLRLASVQLIDLLPPPEQLRLLAPLFSDPLRAIRIEAARAAAGQQDSLAPERLAAWQQ
                     AADEYVATLVYTADRPEGRTALGTFYSRLGRHDEAQAAFAQALALDPRHVPAYLNAAD
                     ASRVQGSDADARRTLELGLAQAPQNAALHHALGLAL"
     gene            complement(2187..3176)
                     /locus_tag="VLN25_00375"
     CDS             complement(2187..3176)
                     /locus_tag="VLN25_00375"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HSQ09063.1"
                     /translation="MSRLFAVVLAFATWAFGPVAIAQTDPLPSWHDGPTKARIVAFVQ
                     AVTERDGKDFVPPADRIAVFDNDGTLWSEQPMYFQLAFAIDRAKAMIAKDPSIAKKPV
                     FKAAATADMKALAATGEKGVMELLALTHANTTTEEFAQTVRDWARTAKHPTLKRRYTE
                     LIYAPMRDLLAYLRANGFKTYIVSGGGIEFLRVISEELYGVPPEQVVGSSIKTKYEVR
                     NGQPVIVRLPEIDFIDDKAGKPVGIHKFIGKRPIAAFGNSDGDFEMLEWTTSAPGARL
                     GVLVHHDDAVREFAYDRMSPVGKLARGLDEAPKRGWTVVSIKDDWTTVYSAPR"
     gene            complement(3236..4786)
                     /locus_tag="VLN25_00380"
     CDS             complement(3236..4786)
                     /locus_tag="VLN25_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HSQ09064.1"
                     /translation="MAFNYSPVVRAASVLLVVLLAAPAALAQPKKPNILVIWGDDIGQ
                     FNISAYNMGMMGYKTPNIDSIAKQGAIFTDWYGQQSCTAGRAAFITGQSPIRTGLTKV
                     GLPGAPEGMKKEDPTIATLLKAQGYVTGQFGKNHLGDRDDMLPTAHGFDEFFGNLYHL
                     NAEEEPENPDYPKSAEFRKKFGPRGVIHSFAGGKITDTGSLTRPRMETIDEEVNAKAI
                     DFMERAKKADKPFFIWWNSTRMHIFTHLKAASEGKTGLGIYADGMAEHDGHVGLLLAK
                     LKELGLEENTIVMYSTDNGAETFTWPDGGTTMFRGEKNTQWEGGYRVPTMIKWPGVIK
                     PGTVINDFGAHEDMLPTLVTAAGGKNFKEDLKKGASIGGRNYKVHLDGYDLGPALRGE
                     AKEWPRREFIYWTDDGQVAALRYDNWKITFLEQQSEGLKVWQMPFTPLRAPVLANLRM
                     DPFERARHEDAMGYQRWYLERMFLIAPAGAFVGQWLQSFKDFPPRQKPGSFNLDRVME
                     AVTSGSKQ"
     gene            4948..>5083
                     /locus_tag="VLN25_00385"
     CDS             4948..>5083
                     /locus_tag="VLN25_00385"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HSQ09065.1"
                     /translation="MLDLRLLHQALTLARHRNFARAADALHVTQPALSRSIAGLETSL
                     G"
BASE COUNT          779 a         1643 c         1707 g          954 t
ORIGIN      
        1 cagagccagg ccgagggcat ggtgcaaggc tgcgttctgt ggtgcctgcg ccagccccag
       61 ctcgagcgtg cggcgtgcgt ccgcatcgct gccctgcacg cgcgacgcgt cggcggcgtt
      121 caaatacgcc ggcacatggc gcgggtcgag cgcaagcgcc tgcgcaaacg ctgcctgggc
      181 ctcgtcgtga cggcccagcc gcgagtagaa cgtgccaagc gctgtgcgcc cctcggggcg
      241 gtcggccgtg tagaccagcg tggcgacgta ctcgtcggca gcttgctgcc acgctgcgag
      301 ccgttctgga gcgagggagt cctgctgtcc ggctgccgca cgcgcggcct cgatgcggat
      361 cgcgcgcagc gggtcggaga acagtggcgc cagcaggcgc agctgctccg gcggcggcag
      421 caaatcgatg agctgtacag aggcgagccg cagcagcggc tgcgcatcgc gcgctgcgct
      481 ccgggcggtg agagcgtcga cgctgccggc ctgcgccagg cgctcgacgg cggaggcccg
      541 cacgatggga gcgtgtgcga ggtcatttgc gatcgtcgcc agcgaggccg tcgcgcccgc
      601 ctgtcccact tcagcggcgc ggaaggacgg cgcgaaaact tggaatcccc tggcgtcccg
      661 accgagccag ccgcgaacgg cggcagcggc ccactttgca tcctgcttcg tgtggcactg
      721 gttgcaggcg ttcggcacgc cgaatgcgac cgattggtcg ggccgcggta cgcgcatgct
      781 gtggtcgcgc cggccgtcga cgaccatgta gagcgtcctg ggcatgtggc agtcggcgca
      841 ttgcgcgcca gcggaggtga ccgagtcggc cgcgtgatga tgatgtgctg gcgtgtcgaa
      901 cttgcccggc gcatgacact ggccgcacac cgcattgccg tcgacgcgca gcttttgcga
      961 gtgcgggtcg tggcagtcgc tgcaggtcac gccctgccga tgcatccggc tctgcagcca
     1021 tgaaccccag ataaagactt catcgcgctg ttggccgtcc acgtgataca gcccgggcag
     1081 cagcagcgac ggcaggtagt gatccatgaa gggctggccg gcccgatagc cctcggcgat
     1141 ctgcgcgcgc cgcgcgtggc attgcgcaca gacctcaatc tcgcgctcgc tggtgcgctc
     1201 gcggctgcgc ctggcgttgc cggtcgcggg gtcgatgctc caggtgactc cgcggcgctc
     1261 gtcgagcaat atcgtcagcc ccttgcttgc gtccgctggc ttcgcctcgg tccaggcgac
     1321 atgggccgag cccggcccgt ggcacgcctc gcagccgacc gaaatctcgt tgaagcgcgt
     1381 gtcgaaggca ttcttgttgg cgtcgtagcc cttcacgacc tgggtcgagt ggcagtccgc
     1441 gcacatgaag ttccagttct gcgcgcgctg cgtccagtgc agttcgtcac ggaagtcgag
     1501 cttctcgttc gggtactggc ggaaccagcg ttggccgccc tgctcctttg ggcgtgcgtc
     1561 ccacgaaacc gccagcgcct gcaggcgacc gcccggcaac tccaccaggt actgctgaag
     1621 ctgctctagt ccgaaggtgt acttcacttc gaattccgcg agcttgccgt cggggccatc
     1681 agtgcgcgcg aagtacttgc cgtcgcgccc gaagaaactc gacgtcacgc catcgaaggt
     1741 gaacttcgcg tcattgaagt cgccgcggac ggtctcggcg gtggcgtgct gcatcgcgcg
     1801 tgcgtgctgt gaggaagtcc acgccgcggt ctcctgcgcg tggcaggcct tgcactcgtc
     1861 gctgccgacg aagagcgccg cgcttggggg actggcggcc gggatcggtg cggaggggga
     1921 cggcgagcgc gtcaattgcc aggccagcgc ggcaaggaga cccgtgaccg ccagagccac
     1981 ccacacccag gccccgcgcg gtgacgccgg cgcagctccc tctctcgtgc ctgactctgc
     2041 ggcggctacc acggccggtg gtgccgacct gttcgggttg cgtttctttg acacgtacga
     2101 gaggtcaatc tgcgagcgat tggatcgcca tactagagat tgttacgagg caacctggcg
     2161 cgcgacggca tgtgcacgcg ctgccgtcag cgtggcgcgg agtagaccgt cgtccagtcg
     2221 tccttgatgc tgacgacggt ccagccgcgt tttggtgcct catcaagccc gcgcgcaagc
     2281 ttgccgacgg gagacatccg gtcgtaggcg aactctcgaa cggcatcgtc gtggtgcacg
     2341 aggacaccaa gccgggcgcc cggtgccgag gtcgtccatt cgagcatctc gaagtcgccg
     2401 tcggagttgc cgaacgcggc gattggccgc ttgccgatga acttgtggat gccgactggc
     2461 ttgcccgcct tgtcgtcgat gaagtcgatc tcgggtagcc gcacgatcac cggttggcca
     2521 ttgcgtactt cgtacttcgt cttgatgctc gatccgacca cttgctccgg cggcacgccg
     2581 tagagttcct cggagatcac gcgcaggaac tcgatgccgc cgccagagac aatgtaagtc
     2641 ttgaatccat tggcgcgcag gtaggcgagc aggtctcgca tcggcgcgta gatgagctcc
     2701 gtatagcggc gcttgagcgt cgggtgcttc gctgttctgg cccagtcgcg aacggtctgc
     2761 gcgaactctt ccgtcgtggt gttggcgtgt gtcagcgcca gcagctccat cacgcccttc
     2821 tcgcccgtgg ccgcgagcgc tttcatgtcg gcggtcgcgg cagccttgaa caccggcttc
     2881 ttcgcgatgc tcgggtcctt cgcaatcatc gccttggcgc gatcgatcgc gaacgcaagc
     2941 tggaagtaca tcggctgctc ggaccacagc gtgccgtcgt tgtcgaacac ggcgatgcgg
     3001 tcggccggcg gcacgaaatc cttgccgtcg cgctcggtca ccgcctgcac gaaggcaacg
     3061 atgcgcgctt tcgtggggcc atcgtgccag gacggcagcg gatccgtttg cgcgatcgcg
     3121 accgggccga atgcccacgt ggcaaatgcc aagacaactg cgaacagacg cgacatcggg
     3181 agctccagcg tgaaacggcg aggggtgggt ccctcgccgt cgcatcgtct cactgttact
     3241 gcttgcttcc gctggtcaca gcctccatca cccggtcgag gttgaagctg cccggcttct
     3301 gacgcggcgg gaagtccttg aagctttgca gccactgacc cacgaacgca cccgcgggcg
     3361 cgatcaggaa catgcgctcc aggtaccagc gttggtagcc catggcgtct tcatgccggg
     3421 cccgctcgaa gggatccatc cgcagattgg cgagcaccgg cgcgcgcagc ggcgtgaacg
     3481 gcatctgcca gaccttcagg ccctcggact gctgctccag gaaggtgatc ttccagttgt
     3541 cgtaacgaag ggcggcgacc tgaccgtcgt cagtccagta aatgaactcg cggcgtggcc
     3601 actccttggc ctcgccacgt agagccgggc caaggtcgta gccgtccagg tgcaccttgt
     3661 agttgcggcc cccgatcgag gcgcccttct tgaggtcttc cttgaagttc ttaccacccg
     3721 ctgcggtcac cagcgttggc agcatgtctt cgtgcgcgcc gaagtcattg atgaccgtgc
     3781 cgggcttgat gacgccgggc catttgatca tcgtcggcac acggtagccg ccttcccact
     3841 gcgtgttctt ctcgccgcgg aacatcgtcg tgccgccgtc gggccaggtg aaggtctcgg
     3901 cgccgttgtc ggtcgagtac atgacgatgg tgttttcctc caggccgagt tccttgagct
     3961 tggcgagtag caggccgacg tgaccgtcgt gctcggccat gccgtcggcg tagatgccga
     4021 ggccggtctt gccctccgat gccgccttca ggtgcgtgaa gatgtgcatc cgcgtggagt
     4081 tccaccagat gaagaacggc ttgtcggcct tcttcgcgcg ctccatgaag tcgatggcct
     4141 tggcattgac ttcctcgtcg atcgtctcca tgcgcgggcg cgtgagcgaa ccggtgtcgg
     4201 tgatcttgcc gccggcaaag ctgtggatga cgccgcgcgg gccgaacttc ttcctgaact
     4261 ccgcgctctt cgggtagtcg gggttctccg gttcctcttc cgcattcagg tgatagaggt
     4321 tgccgaagaa ttcatcgaag ccatgcgcgg tcggcagcat gtcgtcgcgg tcgcccaggt
     4381 ggttcttgcc gaactggcca gtgacgtaac cctgcgcctt cagcagcgtg gcgatggtcg
     4441 ggtcttcctt cttcatgccc tcgggtgcgc ctggcagacc gaccttggtg aggcctgtgc
     4501 ggatcggcga ctggccggta atgaaggcgg cgcggccggc ggtgcagctc tgctgtccgt
     4561 accagtcggt gaaaatagcg ccctgcttcg cgatgctgtc gatgttgggc gtcttgtaac
     4621 ccatcatgcc catgttgtag gcgctgatgt tgaactggcc gatgtcgtca ccccagatga
     4681 cgaggatgtt gggtttcttc ggctgggcga gggcggcggg cgccgctaac agcacaacca
     4741 gcagcaccga tgctgccctg acaaccggcg aatagttgaa ggccatggga ctctcctgaa
     4801 aggtggcgcg cccgcgggga accgagcctg cgctggcgcc ggctgctctt tcagccagtc
     4861 cacccccggc gcgcgaggcg cgaaggtaag ggcacgaggg cgccgggtcc aatgccgctt
     4921 aggcatcgtc tgataacgtc gacgcatatg ctagacctcc ggcttctcca ccaggcgtta
     4981 acgcttgcgc ggcaccgcaa cttcgcgcgg gcggccgatg cgttgcatgt gacgcagccg
     5041 gcgctgtcgc gcagcattgc cggcctcgag acatcactcg gag
//
LOCUS       DATIRT010000019         1590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_887435, whole genome shotgun sequence.
ACCESSION   DATIRT010000019 DATIRT010000000
VERSION     DATIRT010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887435"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1314
                     /gene="creC"
                     /locus_tag="VLN25_00390"
     CDS             <1..1314
                     /gene="creC"
                     /locus_tag="VLN25_00390"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF008312.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HSQ09066.1"
                     /translation="VDTANLLATLLERQAYSGVIQIEELERTLQQLGKRTLNARIFNV
                     DKTRVDLHVYVTDSDGIVLYDSAGRDIGKDYRAWNDVGRTLVGQYGARTTLADPADPA
                     SAVMYVGAAIRERVGAGVPDDIVGMVGVGKPIAAFSPFIANAREKLVIVGVIAVAAFA
                     LLLLMVTVWLVRPFGLVTDLWRALRARPGEARPLGHSLRAAFLDMREALAGKSYVDEY
                     VAALTYEIKSPLAAIRGAAELLREPLPEDARIRFVGNVEEQVKRAQDLVERMLELSGL
                     ERRRALQNRDPVPLAQIADAVRDEMAPIAIQRNVELFIDVDPDLAVSGDRFLLQRALA
                     NLVLNAIEFSPREGTVTVDAHEVRGRIELTVRDHGLGLPSFAHERVFEKFFSLPRPDT
                     GRKGTGLGLAFVREVATLHGGGARLANHPDGGAIATLVLPRPTER"
     gene            complement(1324..>1590)
                     /locus_tag="VLN25_00395"
     CDS             complement(1324..>1590)
                     /locus_tag="VLN25_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HSQ09067.1"
                     /translation="ARELLQGGFLPDVILADMRLGEVIDGIGVVERLRVQLNGPVPAL
                     LISGDTGARELVRVKQSGLLLLTKPVAPARLRSALHALLLATQR"
BASE COUNT          258 a          564 c          524 g          244 t
ORIGIN      
        1 gtcgacacgg ccaacctgct ggcaacgctg ctcgagcgcc aggcctacag cggcgtgatc
       61 cagatcgagg aactggaacg cacgctgcag caactcggca aacgcacgct caacgcgcgc
      121 attttcaacg tcgacaagac acgcgtcgac ctgcacgtct acgtcacaga ctccgacggc
      181 atcgtgctgt acgactccgc cggccgcgac attggcaagg actaccgcgc gtggaacgat
      241 gtcggccgca cgctggtcgg ccagtacggc gcgcgcacga cgctggccga tccggccgat
      301 ccggcgtcgg ccgtcatgta tgtcggggcc gcgatccgcg agcgcgtcgg ggcgggtgtg
      361 cccgacgaca tcgtcggcat ggtcggggtc ggcaagccga tcgccgcatt ctcgcctttc
      421 atcgcgaacg cgcgcgaaaa gctcgtcatc gtcggcgtga tcgccgtggc cgcatttgcg
      481 ctgctgctgc tgatggtgac cgtctggctg gtgcgcccct tcggcctggt gaccgacctg
      541 tggcgtgcgc tacgcgcgcg gccgggtgag gcgcggccgc tgggccattc gctgcgcgcg
      601 gcctttctcg atatgcgcga agcactggct gggaagagct acgtcgacga atacgtcgcg
      661 gccctgacgt acgaaatcaa aagccccctg gcggcgatcc gcggcgccgc cgaactgctg
      721 cgcgaaccgc tgcccgaaga cgcccgcatc cgcttcgtcg gcaacgtcga ggaacaggtg
      781 aagcgcgcac aggacctggt cgaacgcatg ctcgaactgt cgggactgga acgccggcgc
      841 gcgctgcaaa atcgcgatcc ggtgcctctg gcgcagatcg ccgacgccgt gcgcgacgaa
      901 atggcaccga tcgccatcca gcgcaacgtc gagctgttca tcgacgtcga tccggacctc
      961 gcggtctcgg gggaccgctt cctgctgcag cgcgcgctcg cgaacctggt gctcaatgcg
     1021 atcgagttct cgccgcgcga aggcaccgtc accgtcgatg cgcacgaggt ccgcggccgc
     1081 atcgaactga ctgtgcgcga tcatggcctg gggctgccct ccttcgccca cgaacgcgtg
     1141 ttcgagaaat tcttttcgct gccgcgcccg gacaccggcc gcaagggtac cggccttggc
     1201 ctggcctttg tgcgcgaggt cgccaccctg cacggcggcg gcgcccggct ggcaaaccac
     1261 ccggacggcg gcgcaatcgc cacgctcgtg ctgccgcgcc cgacggaacg ctagttcggc
     1321 ggcctagcgc tgcgtcgcca ggagcagcgc gtgcaatgcg ctgcgcagcc gcgccggcgc
     1381 aaccggcttg gtgagcagca gcaatccgct ttgcttcacg cgcaccagct cgcgcgcacc
     1441 ggtgtcgccc gaaatcagca gcgccggcac cggaccgttc agttgaacgc gcagccgctc
     1501 gaccacgccg atgccgtcga tcacttcccc caggcgcatg tcggccagga tgacgtccgg
     1561 aagaaatccg ccctgcagca gttcgcgggc
//
LOCUS       DATIRT010000020         2263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1674173, whole genome shotgun sequence.
ACCESSION   DATIRT010000020 DATIRT010000000
VERSION     DATIRT010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1674173"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VLN25_00400"
     CDS             complement(<1..735)
                     /locus_tag="VLN25_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HSQ09068.1"
                     /translation="MTVLLWAGGLAGAAMVAAALLLTFAFAVVYSNLPPIDALTDYRP
                     KIPLRVWTADGLLIGEFGEERRDYVAVAEMPDLMKKAILAAEDDRFYEHHGVHYLGLL
                     RAAMSNFVSGRRGQGGSTITMQVARNFFLSSERSYIRKIYEIALAYKIESSLSKDRIF
                     EVYANQIFLGQRAYGFAAASQVYFGKKLRDLTVAEMAMLAGLPVAPSAYNPFVNPKRA
                     KSRQEYVLTRMVDLGYIDRPTYETALA"
     gene            1201..>2263
                     /locus_tag="VLN25_00405"
     CDS             1201..>2263
                     /locus_tag="VLN25_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HSQ09069.1"
                     /translation="MAFDLPFATKTPPLIGLDIGSSSVKIVELSEAGKGTMRLERYAI
                     EPLPRGAVVDGNVDKIDVVADAVKRAWRRAGGKIRNVAMALPASAVITKRIALPGNLR
                     EEELEMQVESEANQYIPFALDEVSLDFQVLGPIPNAPDDVEVLIAASRKEKVEDRIAV
                     AQAAGLKPVVMDVESYTARSAIERLIEQLPNSGEGLVIAVFYIGANTTSVTVMHDGEA
                     IYEREQPFGGQQLTGDISRAYGIPTEEAELKKRSGDLPADYETTVLRPFIDTAATEIT
                     RALQFFFTSTPYSRVDQIMLAGGSAVLTGLPDALMERAQVPTSVISPFKGMEVSPNIR
                     DKQLRMDAPALLTPCGLAMR"
BASE COUNT          439 a          687 c          732 g          405 t
ORIGIN      
        1 cgccagtgct gtctcgtagg tgggtcggtc gatgtagccg aggtcgacca tgcgcgtcag
       61 gacgtactcc tgacggctct tcgcgcgctt cggattgacg aacgggttgt aggcgctggg
      121 cgccaccggc aggccggcca gcatggccat ctcggcgacg gtcaggtccc tgagcttctt
      181 gccgaagtag acctgcgagg cggcggcaaa gccataggca cgctggccga ggaagatctg
      241 gttcgcgtag acctcgaaga tgcggtcctt ggacagcgac gattcgatct tgtaggcaag
      301 cgcaatctcg tagatcttgc ggatgtagct gcgctccgag ctgaggaaga agttgcgcgc
      361 gacctgcatg gtgatcgtgc tgccaccctg tccgcggcga cccgacacga agttcgacat
      421 cgcagcacgc aagagcccta agtagtggac gccgtgatgc tcatagaagc ggtcatcctc
      481 ggccgccagg atcgccttct tcatcaggtc cggcatctcg gccaccgcca catagtcgcg
      541 gcgttcctcg ccgaactccc cgatcagcag gccatccgcc gtccagaccc gcagcgggat
      601 cttcgggcga tagtcggtca gggcgtcaat gggcggcagg tttgaataca ccaccgcaaa
      661 ggcgaaggtc agcagcagcg ccgccgcaac catggcggcg cccgccaggc cgccggccca
      721 gagcaatacg gtcacgtacc aggggcgcgc agctccgggc cgggacgatt cgctatcagt
      781 gccgtgcttg tctgcggaca cgggagccgt tgctggaggg aactgggcgt gcgagtgacc
      841 gcttatttgc gcttcgccgc acgaaggttc gggaattata ggagccgagc atcaacggcc
      901 gggaccgatg acgatttccg gcggaccgta agcgttggac gcacctcgtc acgaacggtt
      961 cgttgttaat ccctcaaaag aggcacggtt aggccgaacg cggggcttgt agttgacttg
     1021 tttatgttgc taggatctca agtgctatcc gacaaaaacg cggtaaacgc tcgaaagata
     1081 aggaaaaacc gggtcaaaac cgggcgtttt ggaggccagg gagcgggtgc tcccttcact
     1141 tgtccgcgga tcgctcaatc cgagtgacac cgagctgaga ctgtcgcagg gagtaccgcc
     1201 ttggcatttg atctgccctt tgcaaccaag acgccgccgc tgatcgggct cgacatcggc
     1261 tcgtccagcg tcaagatcgt cgagctgtcc gaggccggca agggcacgat gcggctcgag
     1321 cgttatgcca tcgaaccgtt gcctcgaggt gcggtcgtcg atggaaacgt cgacaagatc
     1381 gatgtcgtcg ccgatgccgt caagcgggcc tggcgtcgcg ccggcggcaa gatccgcaac
     1441 gtggcgatgg cgctccccgc ctccgctgtc attaccaaac gtatcgcctt gcctggcaac
     1501 ttgcgcgagg aagaactcga gatgcaggtc gagagcgagg ccaaccagta catcccgttt
     1561 gcgctcgacg aagtcagcct cgacttccag gtgctgggac cgattcccaa cgcgccggat
     1621 gatgtcgagg tgctgattgc cgcgtcgcgc aaggaaaagg tcgaagaccg cattgcggtg
     1681 gcgcaggcgg caggactgaa gccggtggtc atggacgtag agtcatatac cgcccggtcg
     1741 gcgatcgagc gcctgatcga gcagctgccg aattcgggcg agggcctggt gattgcggtg
     1801 ttctacatcg gcgccaacac caccagtgtg accgtcatgc acgacggcga agccatctac
     1861 gaacgtgagc agccgtttgg tggccagcaa ctcactggcg acatctcgcg tgcgtacggc
     1921 atcccgaccg aggaggcgga actgaagaag cgcagcggcg acctgcccgc tgactacgaa
     1981 acgacagtgc tgcgtccctt catcgatact gcggccacgg aaatcacgcg ggccctgcag
     2041 ttcttcttca cgtcgacccc ctacagccgc gtcgatcaga tcatgctggc gggcggctcg
     2101 gcggtcctta cgggcctgcc ggatgcgctg atggagcgcg cccaggtgcc gacttcggtc
     2161 atctcgccgt tcaagggcat ggaagtgtcg cccaacatcc gtgataagca actgcggatg
     2221 gacgcacctg cgctcctcac gccgtgtgga ctcgcgatgc gga
//
LOCUS       DATIRT010000021         3284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_2715, whole genome shotgun sequence.
ACCESSION   DATIRT010000021 DATIRT010000000
VERSION     DATIRT010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2715"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VLN25_00410"
     CDS             <1..762
                     /locus_tag="VLN25_00410"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HSQ09070.1"
                     /translation="ELLVGWGRRGWMSLRQPVVEAPAEQKPGWWSAKELAGRLGIPEC
                     MPFTDMREYLAARVEKGGFSFAKLESEGVIMGGKEPNTVEEGLEPTFDTPSGKVEFWS
                     DQLAKKGFDPVPKYTKHADAPAGYFRLITGRAPVHTFSRTQSNPLLTGLMRENEIWVN
                     AATATQLGLKSGQYVKLKNQDGLETNRIRVKATQRIRPDTVYMVYGFGQRNSMASSAY
                     QRGASAAQINTRYATDPLMGGTSIHANFVTFVKEA"
     gene            764..1300
                     /locus_tag="VLN25_00415"
     CDS             764..1300
                     /locus_tag="VLN25_00415"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HSQ09071.1"
                     /translation="MARYGMVIDTRKCVGCMDCVVACETENDVPIGFSRDWITTEVRG
                     EFPNLSMEIRSERCNHCENPPCVACCPTGASHVSDLGIVKVTANRCIGCKACIAACPY
                     GARYVHPKGHVDKCTFCDHRIKDGKDPACVSVCPTHCMHFGDLDDPKSSVATLLRTRK
                     WHTLLPEAGTRPRVFYLT"
     gene            1313..2359
                     /gene="nrfD"
                     /locus_tag="VLN25_00420"
     CDS             1313..2359
                     /gene="nrfD"
                     /locus_tag="VLN25_00420"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HSQ09072.1"
                     /translation="MFELTTTRHNPGIDPALHVWGWEIPLYLFFGGMVAGMMVLAGVA
                     MLRTARGEDTKTFFSVQTPLLGFMLLNVGMLALLLDLAHPLYVWAIYITFQALSPMSW
                     GSWVLLIVYGILLVSALIRLPESWPWLGDRVPLLQKASDFFVKHPARMRWLGMANIAF
                     GIAVGIYTGILLNTMVARPLWNTAVLPLLFLFSGLSAAAAAVHLATVVFPGRPAPRGL
                     IGGAFAAMTQPLGPVPPEKRTIDAVIRADVAFLVIELVLIALMLLSLATSTLSHIEAI
                     RFLFSGTYGLVFWVGVIAIGILTPIVLQALELSHRIPHTVVPAVLVLAGGFALRWVLV
                     NAGQASQIVQAAMP"
     gene            2422..2892
                     /locus_tag="VLN25_00425"
     CDS             2422..2892
                     /locus_tag="VLN25_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HSQ09073.1"
                     /translation="MTSRRVSFVAAALLGVAVAFPALVAAQNYPASVKELVANTRKQV
                     KTVDLAGFKNAFDAKTPQLIVDVREPEEFAAGYVPRAVNVPRGLLEFTVWEHVGFPDK
                     LDMNKKIYVYCKTGGRCTLAAKTLQDLGFKDVTSVVMLFEDWKKAGYPVAMPPK"
     gene            2981..>3284
                     /locus_tag="VLN25_00430"
     CDS             2981..>3284
                     /locus_tag="VLN25_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09074.1"
                     /translation="MKVHSLIFGAIALGSAGALAQAPAPKPTQPAVCGNCHKPAPGQV
                     AGYFENVAFKSQAIQLGIDANKEIVRFDPKALKVVDAGEEKKIDHLREVRKGHEARI"
BASE COUNT          604 a         1057 c         1031 g          592 t
ORIGIN      
        1 gagttgctgg tcggctgggg ccgccgcggc tggatgtcgc tgcggcaacc ggtggtcgag
       61 gccccggcgg aacagaagcc gggctggtgg agtgcgaagg aactggccgg ccggctcggc
      121 attcctgagt gcatgccctt cacggacatg cgggagtacc tggccgcccg cgtcgagaaa
      181 ggcgggttca gtttcgccaa actcgagagt gaaggcgtga tcatgggcgg caaagagccg
      241 aacactgtcg aggaagggct cgagccgact ttcgacactc cctccggcaa ggtcgagttc
      301 tggtccgatc agctggcgaa gaagggcttc gacccggtac cgaagtacac caaacacgct
      361 gacgcgcccg cggggtactt ccgcctgatc acgggccgcg ctccggttca caccttcagc
      421 cgcacccagt cgaacccgct gctcaccggc ctgatgcgcg agaacgagat ctgggtcaac
      481 gccgcgacgg ccacgcagct gggattgaag tccggccagt acgtgaagct gaagaaccag
      541 gacggcttgg aaaccaaccg catcagggtc aaggccacgc agcgcatccg ccccgacacg
      601 gtctacatgg tctacggctt cggccagcgc aactcgatgg ccagcagcgc ctaccagcgc
      661 ggagcgagcg ctgcccagat caacaccaga tacgcaaccg atccgctgat gggaggcacc
      721 agcattcacg cgaacttcgt caccttcgtg aaggaggcct gagatggcgc gttacggaat
      781 ggtgatcgac acccgcaagt gcgtcggctg catggattgc gtggtggcgt gtgaaacgga
      841 aaacgacgtg ccgatcggat tcagccgcga ctggatcacg accgaggtgc ggggcgaatt
      901 cccgaacctg tcgatggaga tccgcagtga acgctgcaat cactgcgaga acccgccgtg
      961 cgtggcctgc tgcccgactg gcgcgagcca cgtttctgac cttggaatcg tcaaggtgac
     1021 ggccaaccgc tgcatcggct gcaaggcctg catcgcggcg tgcccctacg gcgcgcgtta
     1081 cgtccacccg aagggccatg tcgacaagtg cacgttctgc gaccaccgca tcaaggacgg
     1141 caaggacccg gcttgcgtct cggtatgccc gacccactgc atgcacttcg gcgatcttga
     1201 cgacccgaag agcagcgtcg ccaccctgct gcggacgcgc aagtggcaca cgctgctacc
     1261 tgaggccggc acccggccac gggtcttcta cctgacgtaa ggagcgcgca ccatgttcga
     1321 attgacgacg acccgccata acccgggcat cgatcctgcg cttcacgtct ggggctggga
     1381 gatcccgctg tatctgttct tcggcgggat ggtcgccggc atgatggtgc tggcgggtgt
     1441 cgcgatgctg cgcactgcca ggggcgagga cacgaagacc ttcttctcgg tccagacgcc
     1501 gctgctcggt ttcatgctgc tgaacgtcgg catgctggcg ctgctgctcg acctggcgca
     1561 cccactttac gtgtgggcca tctacatcac gtttcaggcg ctttccccga tgtcctgggg
     1621 ctcgtgggtg ctgctgatcg tctacggcat cctgctcgta tcggcgctga tacgactgcc
     1681 ggagtcctgg ccatggctcg gcgatcgggt gccgctgctg cagaaggcgt cggacttctt
     1741 cgtcaagcat cccgcgcgca tgcgctggct gggcatggcc aacattgcct tcggcatcgc
     1801 cgtcggcatt tataccggca tcctgctcaa cacgatggtc gcgcgaccgt tgtggaacac
     1861 cgcggtgctg ccgctgctgt tcctgttctc tggactgtcg gcggctgccg cagcagtgca
     1921 cctggcgacg gttgtcttcc cgggccggcc cgcaccgcgc ggactgatcg gcggcgcctt
     1981 cgcggccatg acgcaaccac tcggccccgt gccccccgag aagcgaacca tcgatgcggt
     2041 catccgcgcc gacgtcgcgt tcctcgtaat cgaactggta ctgatcgcac tgatgttgct
     2101 gtcgctggcg acgtcgacgc tgtcgcacat cgaggccatc aggttcctct tcagcggcac
     2161 ttatgggctc gtcttctggg tcggcgtgat cgcgatcggg atccttacgc cgatcgtgtt
     2221 gcaggccctt gagctttcgc accgcattcc gcacacggtc gtgccagccg ttctcgtgct
     2281 cgccggcgga tttgcgctgc gctgggtgct ggtcaacgcc gggcaggcaa gccagatcgt
     2341 gcaggccgcg atgccctagc tccctaagtt cccattcgaa tcagaagatt ccttcaatcc
     2401 atcgcaaaag gagaaacatc gatgacaagc agaagagtct cgttcgtagc cgccgcactg
     2461 ctcggcgtgg ccgttgcgtt ccctgctctc gtggccgccc agaactatcc cgccagcgtc
     2521 aaggaactcg tcgccaacac gcgcaagcag gtgaagaccg ttgacctcgc tggcttcaag
     2581 aatgcgttcg acgcgaagac cccacaactc atcgtcgacg tccgtgagcc ggaggagttc
     2641 gcggcgggat acgttcctcg cgccgtcaac gtgcctcgcg gcctgctcga attcacggtc
     2701 tgggagcacg tcggcttccc cgacaagctg gacatgaaca agaagatcta tgtctactgc
     2761 aagacgggcg gtcgatgcac tctggcagcc aagacgctgc aggacctcgg cttcaaggac
     2821 gtcacctctg tggtgatgct gttcgaggac tggaagaagg ccggctatcc cgtcgcaatg
     2881 cccccgaagt gattgcagcc ctatgactgg tgcaaggccg aacaccgctg tcccgggcct
     2941 cgcgccgcaa tgtccctcac ccacgaggga ggagaaaagc atgaaggtcc actctttaat
     3001 tttcggcgcc atcgcactcg gctcagctgg cgcgctcgcg caggcgccgg cgcccaagcc
     3061 gacgcagccc gcggtatgtg gcaactgcca caagccggcg ccgggccagg tcgccggtta
     3121 cttcgaaaac gttgcattca agtcgcaggc gattcagctg gggatcgacg cgaacaagga
     3181 aatcgtgcgc ttcgacccga aggcattgaa ggtcgtcgat gccggtgagg aaaagaagat
     3241 cgatcacctg cgcgaggtac gcaagggcca tgaggcccgc atcg
//
LOCUS       DATIRT010000022        13002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_494277, whole genome shotgun sequence.
ACCESSION   DATIRT010000022 DATIRT010000000
VERSION     DATIRT010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13002
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494277"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..717)
                     /locus_tag="VLN25_00435"
     CDS             complement(136..717)
                     /locus_tag="VLN25_00435"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSQ09075.1"
                     /translation="MLPDEARPTSNPLARAPSPWVVRFAARLAPGARVLDVASGHGRH
                     ARHLAERGCLVTAVDVDATCATSLSGTPGVHFLHADLENEPWPFADQSYDAVVVVHYL
                     HRPLLPRLTAALAPGGLLIYETFARGNKQFGRPRNPDFLLRPRELIDAFSDLRILAFE
                     DGYVTEPQPAMIQRLSAVRIEPRAYGPVESLAL"
     gene            complement(734..1936)
                     /locus_tag="VLN25_00440"
     CDS             complement(734..1936)
                     /locus_tag="VLN25_00440"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HSQ09076.1"
                     /translation="MFPDRVLSGMRPTGALHIGHYHGALKNWVKLQDEHPCFFFVADW
                     HALTTHYETPEVLEASVWDMVIDWFAAGLDPQKATVFIQSRVPEHAELFLLLSMSTPL
                     GWLERVPTYKDQIDRIKDRDLATYGFLGYPLMQAADILIYRATMVPVGEDQVPHIEMT
                     REIARRFNNLFGREPGFEEKARAAVKKLGAKRAKLYHDLRTRYQEQGDDDALEQARAL
                     LSQAQSMSMGDRERAFGYLEGSRRVILVEPDVKLTETPKLPGLDGQKMSKSYGNMILM
                     REEPKALTDKVRRMTTDPARVRRTDPGEPERCPVWQLHKIYSSDETQAWAAQGCRTAG
                     IGCLDCKQPVIDAMLREQQPWRERAQRYLDDPSLLRSIVADGCDRARATAQDTMREVR
                     SAMGLTYD"
     gene            complement(1952..2599)
                     /locus_tag="VLN25_00445"
     CDS             complement(1952..2599)
                     /locus_tag="VLN25_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HSQ09077.1"
                     /translation="MDGIIQAITVYAIPVLLAITLHEAAHGYVARMFGDSTAYMLGRV
                     TLNPLKHIDPVGTILVPLAIVLVTKLSGGAGFLFGWAKPVPVNFGNLRHPKRDMIWVA
                     AAGPGVNLLMAIAWALLLRGLLSSGIEETFFIEMAKAGVFVNIGLMALNLLPLPPLDG
                     GRILVGLLPHKLAYALSRVEPYGFFILIFLLATNTIGFFLQPFYEFGVGIVRLFI"
     gene            complement(2636..3259)
                     /locus_tag="VLN25_00450"
     CDS             complement(2636..3259)
                     /locus_tag="VLN25_00450"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HSQ09078.1"
                     /translation="MAQLFVVHPIDPQVRLMRQAAQILDHGGVGAIPTDSCYALVCHL
                     DDKAAVDRIRAIRRIDERHLLTMMCRDLSDLASYAKVDNATYRLLRDVTPGPYTLILE
                     ATREVPRRLSHPSRRTIGLRIPDNPVALGLLDVLGQPLISTTLQLPGDELPLNDVDDI
                     RARLDYAIDFVLDAGPCGVEPTTVVDLTAAKAVVVRAGKGDLAALGL"
     gene            complement(3327..4190)
                     /locus_tag="VLN25_00455"
     CDS             complement(3327..4190)
                     /locus_tag="VLN25_00455"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097657 - 3',5'-nucleotide bisphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-nucleoside bisphosphate phosphatase"
                     /protein_id="HSQ09079.1"
                     /translation="MSMHDHPASLVDADLHSHSTVSDGLLTPREVVKRAAEHGVELYS
                     LTDHDEVSGLAEADSAARDVGMPFVHGVEVSVTWASTTIHVVGLGIDPDDRSLQLALA
                     RVRSGRTERAQQMADGLAAAGIDDAYEGALRFVRNPELISRTHFARHLVESGYCRDVR
                     EVFKRYLVDGKPGYVPHEWAHLDEAVKWIASAGGVAVLAHPGRYRIGELKLHELVKEF
                     KEAGGTALEVVTSNHTPDQMRKFARMADEMELEASRGSDFHGPGEAENVELGHVPPLP
                     PGLTPVWHRFT"
     gene            complement(4200..5108)
                     /locus_tag="VLN25_00460"
     CDS             complement(4200..5108)
                     /locus_tag="VLN25_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSQ09080.1"
                     /translation="MPRPSHSRFVDVRGLRYHLRLWGEPQHGSRTLVMLHGWMDVSAS
                     FQFVADALGPGWQVIAPDWRGYGLTERALADCYWFPDYLADLERVLDGLTPDPVVLVG
                     HSMGGNVALLYAGIRPGRVRAVVNLEGFGLRETHASQAPGRYAQWLDELLAGSSLRNY
                     ASPEDVAARLRRNNPRLTAERAEFLARHWALEQADGRWAVAGDPAHRIVNPTLYRLDE
                     VLACWERIACPVLWVRAEGTDVLRFVAENPAAAIDEVERRRAVIPDVEDLVIGDAGHM
                     VHHDQPERVARAIVDFLARKVDAAGR"
     gene            5204..6865
                     /locus_tag="VLN25_00465"
     CDS             5204..6865
                     /locus_tag="VLN25_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSQ09081.1"
                     /translation="MRDHYRALYESFRWNVPVEFNIADVCCRRWANESSRIAMHCEDE
                     QGRSASYTYAALQADANRLSNVLRALGVSRGDRVAIVLPQRPETAIAHIAIYQLGAVA
                     MPLSLLFGPDALEYRLQDSSAVAAIIDPAAAPAIESVRASCPALASLIVVGEHASGAH
                     EWSELLSAASDRFDCVPTQASDPAVLIYTSGTTGPPKGALMSHSALIGNLPGFVASQD
                     WFPRAGDVFWSPADWAWTGGLMDALLPTMYFGFPIVGARGRFAPDRAFELMQRYGVTN
                     SFLFPTALKMMMKSVADPRRQYALRLRSIMSAGEAVGETVFDWCRTSLGVTVNEMFGQ
                     TEMNYIVGNSHEKWPARPGSMGRPYPGHRVAVIDDDGQPLPAGATGEVALNRRDIHGH
                     ADPVFFLEYWRNPGATQRKFSGDWCRTGDLASCDDRGYLWYQGRADDVFKAAGYRIGP
                     SEIENCLIKHPAVANVAVVPRPDAERGAVVKAFVVLAAGHSGSVALVSELQQHVRGKL
                     APYEYPKDIEFIDSLPMTTTGKVQRRVLRELEAARAAESVRNKTA"
     gene            complement(6896..7351)
                     /locus_tag="VLN25_00470"
     CDS             complement(6896..7351)
                     /locus_tag="VLN25_00470"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HSQ09082.1"
                     /translation="MAGARSSPIVGRPYSQFRVGDTFGSTVDITAAHLDRGAELIGDF
                     NPLHVDDEFAQASRFGSRILHGVLTSALMSAPFGNLVAGTALGYLEHNARFLAPVRPG
                     DILTIEWNVVELVAKPHRGGGIVVAECEARNQMGEVVATAHGRMLVADG"
     gene            complement(7359..7730)
                     /locus_tag="VLN25_00475"
     CDS             complement(7359..7730)
                     /locus_tag="VLN25_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09083.1"
                     /translation="MNLVGPVRDRVRTLLKIRVSRGLPSVGTKPALGARLFRDDVRLT
                     VQAGMTDALWRWLVMEGWREITFRPDRRRYRDIPAAYVTRLIDSSADERDRVIAASIA
                     NATVRPLPARRGAWSNTRSRP"
     gene            complement(7796..8158)
                     /locus_tag="VLN25_00480"
     CDS             complement(7796..8158)
                     /locus_tag="VLN25_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09084.1"
                     /translation="MSLASILNTPLSQLLGRRKPAPPRLASAPKARMGATLCCAGLRM
                     TVQAGLTDEMWQWLAALGWRELRSGENRLHYRALSTSLVNRLYDVAPDDRQKILLLAI
                     REATRPQREAATERSASN"
     gene            complement(8239..9018)
                     /locus_tag="VLN25_00485"
     CDS             complement(8239..9018)
                     /locus_tag="VLN25_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HSQ09085.1"
                     /translation="MVGEPEARCRAVDELSEALPLEPERFLAPDRPVPGRPSRPRLVP
                     IHRVPARNPRTADGHAALLHSIAHIEFNAIGLALDAIWRFAGLPPDYYRDWLQVAQEE
                     TRHFRLLSAHLATLGHAYGDFDAHDGLWDMAARTAGDVLDRMALVPRRLEARGLDVSP
                     GIRSKLIAAGDPAAAAILDIILRDEIGHVAIGNRWFNWLCIARGLDPVAADARIALRH
                     RVPRPRGPFNLAARRAAGFSAVEIDLLDQPEAAARAIPSPD"
     gene            9166..9690
                     /locus_tag="VLN25_00490"
     CDS             9166..9690
                     /locus_tag="VLN25_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HSQ09086.1"
                     /translation="MSIYQLGDHVPLVDPTAFVYESATIIGHVIIKSRVNIWPYATIR
                     GDNEPIIIGENSNVQECCVLHTDPGYPLTIGANVTIGHQAVVHGATIGEGCLVGIQAI
                     ILNGAKIGRNCLIGAGALVTEGREIPDNSLVLGSPGKVVRTLDEAAIANMHANTANYV
                     QRGQYYKGALKRVG"
     gene            9706..10614
                     /gene="hslO"
                     /locus_tag="VLN25_00495"
     CDS             9706..10614
                     /gene="hslO"
                     /locus_tag="VLN25_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015602261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HSQ09087.1"
                     /translation="MSDRLLKFLFRGAPVRGELVRVTDAWREMTAHHDYPAPVKRLLG
                     EMVAAAALLASNIKFNGALVMQIHGDGPVKLLVVECQPDLALRATAKLGDESVDAEAT
                     MRELINRGGRGRFAITLDPRDPLPGQRPYQGVVPLDGETMADVIETYMRQSEQLETKL
                     VLAANDEASAGLLLQRLPDHGGSETVIDADAWDRATTLGATLRSEELLRLTPENVARR
                     LYGQEQLDHQATLAPRFACTCSRERIGRMLNSLGRDEVDSIIEELDTVTVTCDFCNRQ
                     YSFDAVDVVQLFASGQTGEGDRSQRH"
     gene            complement(10654..11967)
                     /locus_tag="VLN25_00500"
     CDS             complement(10654..11967)
                     /locus_tag="VLN25_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSQ09088.1"
                     /translation="MNLHRLLKQREERGEPLRVGLIGAGKFGSMFLSQVRHTPGMRVV
                     AIADLTPQRAREALKTVGWSESTISAVRYFDDARRMIDEPDVDIVIDATGSPSAGIDH
                     VLACCERGKHIVMVNVEADALAGPLLALRAQQAGIVYSLAYGDQPALICEMVDWARAC
                     GFEVVAAGKGTKYLPEYHASTPATVWKYYGLSEDDARRGGLNAQMFNSFLDGTKSAIE
                     MCAVANATGLHAPEGLSFPPCGVDDLPTVLRPVSEGGVLHQRGQVEVISSLKRDGSPV
                     TRDLRWGVYVVFSGGSEYVERCFKEYGLVTDPSGQYSALYKPFHLIGLELGISVASVG
                     LRGEPTGAPIGFNADVVATAKRDLAPGEMLDGEGGATVYGRVLPAEESLRLEGLPLGL
                     AHHVRLHKAVPAGQPVRWTDVEIDEASPAVQFRRTMEKTEFGETV"
     gene            complement(11998..12306)
                     /gene="ftsB"
                     /locus_tag="VLN25_00505"
     CDS             complement(11998..12306)
                     /gene="ftsB"
                     /locus_tag="VLN25_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HSQ09089.1"
                     /translation="MRILGIVLAVLLVAIQYPLWFGKGGWLRAWELERQLAAQRESNS
                     TLAARNAAAAAEVSSLREGNEAIEERARSQLNMVKEGEVFFQFVDRAPAAAPASEPQR
                     "
     gene            complement(12310..>13002)
                     /gene="eno"
                     /locus_tag="VLN25_00510"
     CDS             complement(12310..>13002)
                     /gene="eno"
                     /locus_tag="VLN25_00510"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525887.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HSQ09090.1"
                     /translation="AVGDEGGFAPSVASHEAAIQLILQAIDSAGYTAGSQIAIALDCA
                     ASEFYKDGAYRLDGEGLELSAADWTNMLATWCDKYPILSIEDGMAENDWDGWVTLTER
                     LGTRVQLVGDDLFVTNTAILREGIRKGVANSILIKINQIGTLTETFAAIEMAKCAGYT
                     SVVSHRSGETEDATIADIAVGTNAMQIKTGSMSRSDRIAKYNQLLRIEEDLGDIASYP
                     GRGAYYNLHNLR"
BASE COUNT         2266 a         4326 c         4243 g         2167 t
ORIGIN      
        1 ccgtcttccc gcatgggcga aacgatggcg acgatgctgc ccttgatcat ggtgtctgcg
       61 gtctccctgg ccgaagcgcg gaattctacc gtgcagcgct cggccgccgg cccacaccgc
      121 gttcggccct gcacactaca gcgcgagcga ttcgaccggg ccgtaggccc gcggttcaat
      181 tcgcacggcg gacagtcgct ggatcatggc tggctgtggc tcggtgacgt agccatcctc
      241 gaaggcgagg atgcgcaaat cgctgaacgc gtcgatcagc tctcgcggac gcagcaggaa
      301 atcggggttg cgcggccggc cgaactgctt gttgccgcgt gcgaaggtct catagatcag
      361 caggccgccc ggggccagcg ccgccgtcag tcgaggcagc aggggccggt gaagatagtg
      421 cacgacgacg accgcgtcgt agctctggtc agcgaacggc caaggctcgt tctcgagatc
      481 ggcgtgcagg aagtgaactc ctggcgtccc ggaaagcgac gttgcgcacg tcgcgtcgac
      541 atccaccgcc gtgacgaggc agccgcgctc agctaagtgg cgcgcgtggc gcccatggcc
      601 acttgcaacg tcgaggacac gggctcccgg cgccaggcgt gctgcgaagc gcacgaccca
      661 gggcgacggc gcgcgcgcaa gcgggttgga agtcggccga gcctcgtccg gcagcaacgc
      721 acttgcctcc cgcctaatcg taggtaagcc ccatggcgct gcggacctcg cgcatggtgt
      781 cctgcgccgt cgcgcgggcg cggtcacaac catccgcgac gatggatcgc agcagcgacg
      841 gatcgtccag gtagcgctgg gcccgttcgc gccagggctg ctgttcccta agcatcgcgt
      901 cgatgaccgg ctgcttgcag tcgaggcagc cgatgcccgc ggtacggcag ccctgcgccg
      961 cccaggcctg cgtctcatcg ctcgaataga tcttgtgtag ctgccacacc gggcagcgct
     1021 cgggttcgcc cggatcggtg cgtcgcacac gcgccggatc ggtcgtcatg cgacggacct
     1081 tgtccgtcag cgccttgggc tcctcgcgca tgaggatcat gttgccgtag ctcttggaca
     1141 tcttctggcc atcgagtccg ggcagcttgg gagtctcggt gagcttcacg tccggctcga
     1201 ccaggatgac gcgccgcgaa ccctcgagat aaccgaaggc acgctcgcga tcgcccatcg
     1261 acatgctctg tgcctgcgac agcagcgcgc gcgcctgttc cagcgcgtca tcgtcgccct
     1321 gctcctggta gcgcgtacgc aggtcgtgat agagcttcgc gcgcttggcg ccgagcttct
     1381 tgaccgccgc cctggccttc tcctcgaaac cgggctcgcg accgaagaga ttgttgaagc
     1441 ggcgcgcgat ctcgcgcgtc atttcgatgt gcggcacctg gtcctcgccg accggcacca
     1501 tggtcgcgcg gtagatcagg atgtctgcgg cctgcatcag agggtagccg aggaagccgt
     1561 aggtcgcgag gtcgcggtcc ttgatcctgt cgatctggtc cttgtacgtc gggacgcgct
     1621 cgagccagcc gagtggcgtc gacatcgaca gcagcaggaa gagttccgcg tgttcgggca
     1681 cacgcgactg gatgaagacg gtggccttct ggggatcaag gcccgcggca aaccagtcga
     1741 tcaccatgtc ccagacgctg gcctcgagta cttccggtgt ttcgtagtgc gtcgtcagcg
     1801 catgccagtc ggcgacgaag aagaagcacg ggtgctcgtc ctgcagcttg acccagttct
     1861 tcagcgcacc gtggtagtgg ccgatatgca gcgcgcccgt cgggcgcatg ccggaaagca
     1921 cgcggtcagg aaacatggct cagacgaaca ttcagatgaa cagtcggacg atgccaacgc
     1981 cgaattcgta gaacggctgc aggaagaagc caatcgtgtt ggtcgccagc aggaagatca
     2041 ggatgaagaa cccatacggt tcaacgcgcg acagcgcgta cgccagcttg tggggcagca
     2101 ggccgacgag aatacggccg ccgtccagcg gcggcaacgg cagcaggttg agcgccatca
     2161 gtccgatgtt gacgaacacg cccgccttgg ccatctcgat gaagaatgtc tcttcgatgc
     2221 ctgacgacag caggcccctg agcagcaggg cccacgcaat cgccatcagc aggttgacgc
     2281 cgggaccagc tgcggcaacc cagatcatgt cgcgcttcgg atgccgcagg ttgccgaagt
     2341 tgaccggcac cggcttggcc caaccgaaga ggaagcccgc cccgcccgaa agcttcgtga
     2401 ccaatacgat cgccagcggc acgagaatgg tgccgaccgg gtcgatatgc ttgagaggat
     2461 tcagcgtcac gcgcccgagc atgtaggccg tcgagtcgcc gaacatgcgg gcgacatagc
     2521 catgggccgc ttcatgcagg gtgatggcca gcagcaccgg gatcgcgtag acggtgatcg
     2581 cctgaatgat gccgtccatc gcaatcagtc tagcagccgc ccccgccgga ccctttcaga
     2641 gaccgagagc cgccagatca cccttgccgg cccgcacgac caccgccttc gcggccgtga
     2701 gatcgacgac cgtcgtcggc tccactccgc acggtcccgc atcgagcacg aagtcgatcg
     2761 catagtcgag gcgcgcgcga atgtcgtcga cgtcgttcag cggcagttca tcgcccggca
     2821 actgcagtgt cgtgctgatc agcggctggc ccaggacgtc gagcagtcca agtgccacgg
     2881 ggttgtctgg aatgcgcagg ccgatcgtcc ggcgcgacgg gtgcgaaagc cgccgcggca
     2941 cttcgcgcgt cgcctcgagg atcagtgtgt agggtcccgg cgtcacatcc cgcagcagtc
     3001 ggtaggtggc gttgtcgacc ttcgcgtagg acgcaaggtc ggacaggtcg cggcacatca
     3061 tcgtcagcag gtgacgttca tcgatgcggc ggatcgcgcg aatgcggtcc accgccgcct
     3121 tgtcgtccag atggcagacg agcgcgtaac acgagtcggt aggaatcgcg ccgacgccgc
     3181 cgtggtcgag tatctgcgcg gcctgccgca tcagtcgcac ctgtggatct atcggatgaa
     3241 cgacgaacaa ctgcgccatg gctcagcccc gcccggcgac ggccgccgcg ctcatggagc
     3301 gctcatggag cgaaacacgt ccggcctcac gtgaagcgat gccagaccgg cgtcagtccc
     3361 ggaggcaacg gcgggacatg tccgagttcg acgttctccg cctcgccggg cccatgaaag
     3421 tcactgccgc gcgacgcctc gagttccatc tcgtcggcca tgcgtgcgaa cttccgcatc
     3481 tggtccggag tgtgattgct ggtgacgact tcgagcgctg tcccgccggc ctccttgaac
     3541 tccttcacga gttcatgcag cttcagttcc ccgatgcggt agcgcccggg atgggccagc
     3601 accgcgacgc cgccggcgga ggcgatccac ttcacggcct cgtcgaggtg cgcccactcg
     3661 tgcggaacgt aacccggctt gccgtccact aggtaacgct tgaacacttc gcgcacgtcg
     3721 cggcagtatc ccgactcgac cagatgccgc gcgaagtgcg tacgcgaaat cagctcggga
     3781 ttgcggacga atcgcagcgc accttcgtac gcgtcatcga tgccggcggc tgcgagcccg
     3841 tccgccatct gctgtgcgcg ttcagtacgt ccactgcgaa cgcgcgccag cgccagttgg
     3901 agcgagcgat cgtcgggatc gatgccgagt ccgacgacat ggatggtcgt cgacgcccag
     3961 gtgacggaaa cctcgacgcc atggacgaac ggcatgccga cgtcgcgggc ggcgctgtcc
     4021 gcttcagcca gcccggacac ctcgtcatgg tcggtcaacg aatacagttc gaccccatgc
     4081 tcggccgcgc gcttgacgac ctcgcgtggc gtcaacaggc cgtcggatac ggtggaatgg
     4141 gagtgaaggt ctgcgtcaac caggctcgcc ggatgatcat gcatcgacat cacggcattc
     4201 taccggccgg ctgcgtcgac ctttcgtgca aggaagtcga cgatggctcg cgccacgcgt
     4261 tccggctggt cgtgatggac catgtgaccg gcatcaccga tgacgaggtc ttcgacgtcc
     4321 gggattacgg cgcgccgccg ttccacctca tcgatggccg ccgccggatt ctccgcgacg
     4381 aagcgcagca catcggtgcc ctcggcgcgt acccagagca ccggacaggc gatgcgctcc
     4441 cagcaggcca gcacttcatc caggcgatac agcgtcggat tgacgatgcg gtgcgccggg
     4501 tcgccggcca cggcccagcg gccgtcggcc tgctccagcg cccaatgccg ggcgagaaat
     4561 tcagcgcgct cggccgtcag tcgcggattg ttccgtcgca ggcgcgccgc gacgtcctcc
     4621 ggactcgcat agttgcgcag cgacgaaccg gccagaagtt cgtcgagcca ttgcgcatag
     4681 cggcccgggg cctgggaggc gtgcgtctca cgcaggccga agccctcgag gttgacaacc
     4741 gcacgtaccc ggccgggccg gatgcccgca tacagcaggg ccacgttgcc acccatgctg
     4801 tggccgacca gcacgacggg gtccggcgtg aggccgtcga gcacccgctc gaggtccgca
     4861 aggtaatcgg gaaaccagta gcaatcggcg agcgcacgct cggtgaggcc gtagccgcgc
     4921 cagtcgggcg caatcacctg ccagcccggg ccgagcgcat ccgcgacgaa ctgaaacgag
     4981 gccgatacgt ccatccagcc gtgcaacatg accagcgtac ggctgccatg ctgcggctcg
     5041 ccccagagcc gcaggtgata gcgcaggccg cgaacgtcga cgaagcggga gtgcgatggg
     5101 cgcggcatgg cgatcaggtg acctctcggc ccgcgagcgt ttgcaggggt ggcagggttc
     5161 ggcgattata ggaaccggcc cgcccgagac agagaggatc accatgcgcg accactatcg
     5221 cgcgctgtac gagtcgttcc gctggaacgt gccggtcgaa ttcaacattg ccgacgtgtg
     5281 ctgccgtcgc tgggccaacg agtcgtcgcg tatcgctatg cactgcgagg acgaacaagg
     5341 gcggtcggcg tcttacacct acgccgcgct gcaggccgac gccaaccgac tgtcgaatgt
     5401 gctgcgggcg ctgggcgtat cgcgcggcga ccgcgtggcc attgtcctgc cgcagcgacc
     5461 cgaaacggcg atcgcgcaca tcgcgattta ccagctgggc gccgtcgcga tgccactttc
     5521 gctgctgttc ggccccgacg cgctggagta tcggctgcag gacagcagtg ccgtcgccgc
     5581 gatcatcgat ccggctgcag cgcccgccat cgagtcggtg cgcgcgagct gtcccgcatt
     5641 ggccagcctg atcgtcgtgg gcgagcacgc atccggtgcc cacgagtgga gcgaactgct
     5701 atccgcggca tcggaccgat tcgattgcgt accgacacag gccagcgatc cggcggtgct
     5761 gatctacacg agcggaacca caggaccgcc caagggtgca ttgatgtcgc actcggcgct
     5821 gatcggcaat ttgccggggt tcgtggcgtc gcaggactgg tttccgcgcg ccggcgacgt
     5881 gttctggtcg cctgccgatt gggcctggac gggcggcctg atggatgcac tgttgccgac
     5941 gatgtacttc ggcttcccga tcgtcggcgc gcgcggacga ttcgcgccgg accgcgcttt
     6001 cgaactgatg caacggtacg gcgtgaccaa ctcgttcctg tttccgaccg cgctgaagat
     6061 gatgatgaag tcggtcgctg atccgcggcg acaatacgcg ctgcgcctgc gttccatcat
     6121 gagtgcgggc gaggcggtcg gcgagacggt gttcgactgg tgccgcacgt cgctcggcgt
     6181 gacggtcaac gagatgttcg gccagaccga gatgaactac atcgtcggaa actcgcacga
     6241 aaagtggccg gcgaggccag gcagcatggg tcgcccgtac ccgggtcatc gggtggcggt
     6301 gatcgacgac gacggccagc cgttgcccgc tggagccacc ggagaagtcg cgctgaatcg
     6361 tcgcgatatc cacggccatg ccgatcccgt gttcttcctc gaatactggc gcaatcccgg
     6421 cgcaacgcag cgcaagttca gcggcgactg gtgccgcacg ggtgacctgg cgagctgtga
     6481 cgaccggggc tacctctggt accagggacg cgccgacgac gtcttcaagg ccgctggata
     6541 tcgcatcggt cccagcgaaa tcgagaactg cctgatcaag catccggcag tcgccaacgt
     6601 ggccgtggtg cctcggccgg atgccgagcg cggggccgtg gtcaaggcgt ttgtcgtgct
     6661 ggcggccggt cacagcggct cagtcgctct ggtgtccgag ttgcagcagc acgtgcgcgg
     6721 caagctcgcg ccttacgagt acccgaaaga catcgagttc atcgactctt tgccgatgac
     6781 caccaccggc aaagtgcagc gccgcgtact gcgcgaactc gaggctgcgc gggctgcgga
     6841 gtcggtcagg aacaaaacgg cttgagcggc gcaagggcgc gaccgcgttg cgcatctagc
     6901 cgtcggcgac gagcatcctg ccgtgggcgg tggcgaccac ttcgcccatc tggttacgcg
     6961 cctcgcactc ggctacgacg atgcctccgc cgcgatgcgg cttcgccacc agttcgacga
     7021 cgttccattc aatggtgagt atgtcgcctg gacgcaccgg tgcgaggaag cgcgcgttgt
     7081 gctcgaggta accgagcgcc gtgcctgcga ccagatttcc gaacggtgcg ctcatcaaag
     7141 ctgatgtcag cacgccgtgc aggatgcggc tgccgaagcg cgacgcctgg gcgaactcgt
     7201 catcgacgtg cagcgggttg aagtccccga tgagttctgc gccgcgatcg agatgggcgg
     7261 cggtgatgtc aaccgtcgaa ccgaacgtgt ctccgacacg gaactgggag taggggcggc
     7321 cgacgatcgg ggacgaccgc gcgcccgcca tagacgaact agggtcggct gcgggtgttg
     7381 gaccaggcac cacgccgcgc gggcagtggc cgcaccgtgg cattggcgat cgatgcggca
     7441 atgacgcggt cgcgttcgtc cgccgacgag tcgatcaggc gggtgacata ggcggcagga
     7501 atgtcgcggt agcggcggcg gtccggacgg aacgtgatct cacgccagcc ttccatgacg
     7561 agccagcgcc acagggcatc cgtcatgcct gcctgtacgg tcaggcgcac gtcatcacgg
     7621 aacaggcgcg cacccagggc cggcttggtt cccaccgacg gcagaccgcg ggacacgcgg
     7681 atcttcagca gcgtccgaac acgatcacgt acaggtccaa ccagattcac ttcgatgcaa
     7741 tcctccgagc acgaacttta tcgacacagg ctatccgacg tatcccccga aagggctagt
     7801 ttgacgcact gcgctccgtt gctgcttcgc gctgggggcg cgtcgcctcc cggatcgcca
     7861 gcaggagaat cttctgccgg tcgtccggtg ccacatcgta aagccggttg acgagcgagg
     7921 tggacagggc acggtagtga aggcggttct cgcccgatcg cagttcccgc cagcccagcg
     7981 ccgccagcca ttgccacatt tcgtcggtca gccccgcctg caccgtcatg cgcagcccgg
     8041 cacaacacag cgttgctccc atgcgcgcct tcggcgcgct ggcgagccgc ggcggagcgg
     8101 gtttcctgcg gcccagcaac tgactcagcg gggtgttcag tattgaagcc agactcatgt
     8161 gtcctccggg agtgattagc actgtatcgg tgccgtcggt gcgattcgat cccacgtggg
     8221 tgcgatcgcg gctggaggct agtccggaga gggtattgcc cgggcagcgg cctccggctg
     8281 atcgagcagg tcgatttcca ctgcggagaa acccgcggcg cgccgtgccg caaggttgaa
     8341 tggcccgcgc ggtcgcggca cgcgatgacg caacgcaatg cgggcatctg cggcgaccgg
     8401 atccagcccg cgggcaatgc agagccagtt gaaccagcga ttgccgatgg cgacatggcc
     8461 gatctcgtcc cgcaggatga tgtcgaggat cgccgcagcc gccggatcac cggccgcgat
     8521 cagtttggac cggatcccgg gggaaacatc cagaccgcgg gcttcgagcc ggcgtggcac
     8581 gagcgccatg cggtcgagca catctccggc ggtgcgcgcc gccatgtccc acaagccgtc
     8641 atgcgcatcg aagtcgccat aggcatgacc cagcgtcgca agatgcgcgc tcagcagtcg
     8701 gaagtggcgc gtttcctcct gggcgacttg cagccagtcg cggtaatagt ccggcggcag
     8761 tccggcgaag cgccagatcg catcgagcgc cagtccgatc gcattgaatt cgatgtgtgc
     8821 gatcgagtgc agcagggctg cgtgtccgtc cgccgttcgc gggttgcgtg ccgggacccg
     8881 gtggattggt accaggcgcg gacgagacgg tcgtcccggt accggtcgat ccggcgcgag
     8941 gaaccgttcg ggctcgagcg gcaacgcttc ggacaactcg tccactgccc ggcaacgcgc
     9001 ctcgggctcg cccaccacga gggcctgcag cgccagggtc cgcagttcgc tcacgcggat
     9061 ctcttcgatc ggctcggcgc gggccgcctc tcgagtcccc gtaaactgac gttgagtctc
     9121 cttctcacat tccaccctga accaaccgtt gatcgggctc ccgtcatgag catctaccag
     9181 cttggggacc acgtcccgct tgtcgacccc acggcattcg tttacgaaag cgccacgatc
     9241 atcggccacg tcatcatcaa gtcgcgcgtc aacatctggc cgtacgcgac gatacgcggt
     9301 gacaacgagc ccatcatcat cggcgaaaac agcaacgtgc aggagtgctg cgtcctgcac
     9361 acggaccccg ggtatccgct gaccatcggt gccaacgtca cgataggcca ccaggccgtg
     9421 gtgcacggtg cgacgatcgg cgaaggatgc ctcgtcggca tccaggcgat catcctcaac
     9481 ggcgcgaaga tcggccgcaa ctgcctgatc ggggccggcg cactcgtcac cgagggtcgc
     9541 gagattccag acaactccct agtcctcggc tcgcccggca aggtcgtgcg cacgctcgat
     9601 gaagcagcca tcgcgaacat gcacgccaat accgcgaatt acgtacagcg cggccagtac
     9661 tacaagggcg cgctcaagcg cgtcggctga cgagtccggg ggtccatgtc cgatcgcctg
     9721 ctgaaattcc tgtttcgcgg tgcaccggtg cgcggagaac tcgtccgcgt cacggacgcg
     9781 tggcgcgaga tgacagcgca ccacgactac cctgccccgg tcaagcgcct gctcggcgaa
     9841 atggtcgctg ccgcggccct gctggcgagc aacatcaagt tcaacggcgc cctcgtaatg
     9901 cagatccacg gcgacggccc ggtgaagctc ctcgtcgttg agtgccagcc cgatctcgcg
     9961 ctccgtgcga ccgccaagct cggagacgag tcagtggacg ctgaggcgac gatgcgcgag
    10021 ctgatcaacc ggggcggccg cgggcgcttc gcgatcacgc tcgatccgcg cgatcccctg
    10081 ccgggacagc gcccgtacca gggagtcgtc ccgctcgacg gcgagacgat ggccgatgtg
    10141 atcgagacct atatgcggca gtcagaacag ctcgagacaa agctggtgct cgccgcgaac
    10201 gacgaggcca gcgcgggact gctgctgcag cgcctgcccg accacggcgg atcagaaacg
    10261 gtcatcgatg cagacgcatg ggatcgcgca acgacgctcg gtgccacctt gcgcagcgag
    10321 gaactgctgc gcctgacccc cgagaacgtg gcacggcggc tgtacggcca ggagcagctc
    10381 gaccatcagg ccacgctggc accgcgcttt gcctgcacgt gttcgcgcga acggattggc
    10441 cgcatgctga actccctagg gcgcgacgaa gtcgactcga tcattgagga gctcgacact
    10501 gtgacggtca cgtgcgattt ctgcaaccgt cagtattcat tcgatgcggt cgatgtggtg
    10561 cagctgtttg ccagcggcca gacgggggaa ggcgacaggt cccagcggca ttgatccgtc
    10621 gcggccggcg gacgcaacgg gacgaccccc cgttcaaacg gtctcgccga actccgtttt
    10681 ctccatcgtt cggcggaact ggaccgcggg tgacgcttca tcgatctcga cgtcggtcca
    10741 gcgcaccggc tggccggccg gcacagcttt gtgcagccgc acgtgatggg ccaggccgag
    10801 cggcagccct tctagcctca gcgattcttc ggcaggcaac acacggccat agacggtggc
    10861 tccgccctcg ccgtcgagca tctcgcccgg cgcgaggtcc cgcttggccg ttgccacgac
    10921 atccgcattg aatccgatcg gcgctccggt cggctcgccg cgcaggccga cgctcgcgac
    10981 actgatgccg agttcgaggc cgatcagatg aaatggcttg tacagcgccg agtactgccc
    11041 gctcgggtcg gtcaccagtc cgtactcctt gaagcagcgt tccacgtact cgctgccgcc
    11101 cgagaacacg acgtacacgc cccaccgcag gtcacgagtc acggggctgc cgtcacgctt
    11161 gaggctggag atcacttcga cctgtccgcg ctgatgcagc actccgccct cactcaccgg
    11221 ccgcaacacg gtcggcagat catcgacgcc gcacggcggg aagctcaggc cctcgggcgc
    11281 atgcagcccg gtcgcattgg ccaccgcgca catctcgatg gcgcttttcg tgccatccag
    11341 gaacgagttg aacatctgcg cattcagacc gccgcgacgt gcgtcgtcct cactcagccc
    11401 gtagtacttc cagacggtcg cgggggtcga ggcgtggtac tcgggcaggt acttcgtccc
    11461 cttgccggca gcgacaacct caaagccgca ggcgcgcgcc cagtcgacca tctcgcagat
    11521 caaggccggc tggtcaccgt aggccagcga gtacacgatg cctgcctgct gggcgcgcag
    11581 cgccagcagc gggcccgcca gcgcgtcggc ctcgacgttc accatgacga tgtgcttgcc
    11641 acgctcgcag caggcaagca cgtggtcaat gccggcggac ggcgatcccg tcgcatcgat
    11701 cacgatgtcg acgtcgggct catcgatcat gcgccgcgcg tcgtcgaagt agcgaacggc
    11761 ggagatggtc gactccgacc agccgaccgt cttcagcgcc tcgcgtgcgc gctgcggcgt
    11821 caggtcggca atcgccacga cccgcatgcc cggcgtgtgt cgcacctgcg aaaggaacat
    11881 cgatccgaac ttgccggcgc cgatcagccc cacgcgcaga ggctcaccgc gctcctcgcg
    11941 ttgcttcagc aatcgatgaa gattcacgcc actcctccgt gtcccgggcg cctgcgctca
    12001 gcgttgcggt tcgctggccg gggctgcggc cggcgctcga tcgacaaact ggaagaacac
    12061 ttcaccttcc ttgaccatgt tgagctgcga gcgcgcgcgt tcctcgatcg cctcgttgcc
    12121 ttcacgcagg ctggagacct ccgcggccgc cgcggcgttt cgcgcggcaa gtgtggaatt
    12181 ggactcgcgc tgtgccgcga gctggcgctc cagttcccag gcgcgcaacc agcccccctt
    12241 gccgaaccac agcggatact ggatcgctac cagcaggacg gccagcacga tgccgagtat
    12301 ccgcatcgat cagcgcaggt tgtgcaggtt gtagtaagca ccgcgcccag gatagctcgc
    12361 gatgtcgccg agatcctctt cgatgcgcag cagctggttg tacttggcaa tacggtccga
    12421 gcggctcatc gaacccgtct tgatctgcat cgcgttggtg ccgaccgcga tgtcggcaat
    12481 cgttgcatct tccgtctcgc cggaccggtg gctgacgacg gaagtgtagc ccgcacactt
    12541 cgccatctcg attgcagcaa aggtctcggt cagggttccg atctggttga tcttgatgag
    12601 gatcgagttg gccactccct tgcgtatgcc ctcgcgcagg atcgcggtat tcgtcacgaa
    12661 aagatcgtcg ccgaccagct ggaccctggt tccgagccgc tcggtgagtg tgacccatcc
    12721 gtcccagtcg ttctccgcca tcccgtcctc gatcgacagg atcgggtact tgtcgcacca
    12781 ggtggccagc atgttcgtcc agtccgccgc tgacagctca agtccctcac cgtcgaggcg
    12841 gtaggcaccg tccttgtaga actcgctcgc tgcgcagtcg agcgcgatcg cgatctgcga
    12901 gccggcggtg tacccggcgc tatcgattgc ctgcaggatc agctggatgg ccgcttcgtg
    12961 gctggcgacg ctgggcgcga acccgccttc gtcgccgacc gc
//
LOCUS       DATIRT010000023         7762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_396079, whole genome shotgun sequence.
ACCESSION   DATIRT010000023 DATIRT010000000
VERSION     DATIRT010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7762
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396079"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3304)
                     /gene="dnaE"
                     /locus_tag="VLN25_00515"
     CDS             complement(<1..3304)
                     /gene="dnaE"
                     /locus_tag="VLN25_00515"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HSQ09091.1"
                     /translation="MSGTQFVHLRLHSEFSVVDGIVRIDDAVAKAAADGQGALALTDS
                     ANVFGAVRFYQAARRKGVKPILGCDVWVTNDVDRDNPYRLLLLAQDRSGYLNLCTLLT
                     RAFLENQHRNRAELRLDWLEADSGACAAGLIALSGARLGEVGAHLMASRPDAAILAAQ
                     RLAALFPGRFYLELQRTGDARDAACTHATLVLALKQHLPVVATHPIQFLVPDDFRAHE
                     ARYCIAEGYTLADPRRPRRFTREQYFKTRSEMAVLFADVPAALANAVEIARRCNLTLE
                     LGKARLPPFPTPVGVTLDDHCRDLTGTGLEQRLQSLYPDDTVRAQRRPEYAARLDLEL
                     KTIAKMGFSGYFLIVADFINWAKGNGIPVGPGRGSGAGSLVAYSLGITDLDPLRYGLL
                     FERFLNPERVSMPDFDIDFCQDNRDRVIDYVKQKYGAAAVSQIATFGTLGAKAVVRDA
                     GRVLDLPYTKCDQLSKLIPHNPADPWTLDRALREEPAFAEAVNSDEENQELIALARPL
                     EGLTRNVGMHAGGVLIAPGKLTDFCPLYCAPGAQGAAISQFDKDDVEAIGLVKFDFLG
                     LTTLTILDLTLQYVRQLDPAFNLRLDTLPLDYAKTYDVFKQAATAAIFQFESRGMREL
                     LKRAKPDRLEDLIALNALYRPGPMDLIPDFIERKHGRQRVEYLHPSVEPILAETYGVM
                     VYQEQVMRIAQVVGGYSLGGADLLRRAMGKKKPEEMAKQRSIFVEGAGKQGVEANTAT
                     ELFNLMEKFAGYGFNKSHSAAYAVVAYQTAYFKAHYPAAFMAANLTAVKDDTDKVKDL
                     IEDCKALRLTVQAPDVNAGGWRFEPVDMQTIRYGLGAIKGTGQGAIEAILAARSTGGP
                     FESLFDLCARVDKHVVNRRVVEALVRAGALDRLHDDRATLLATVGRAIEAADSAAASA
                     GQASLFGGGEASVRHIDYVRAAPWSERERLTNEKLALGYYFSGHLFREYATEARLLAP
                     TPLVEVRQARDQVRLTGVIVSARSQNTRRGRMGVVVLDDGTAQLELMVFSELYDRKRA
                     LLKEDQLLCVVGKVRFDEFAQRLSISADDLMDLTEARANAAARLWIELDGAGDAARLR
                     EALSPYRVPGNGS"
     gene            3483..3974
                     /locus_tag="VLN25_00520"
     CDS             3483..3974
                     /locus_tag="VLN25_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09092.1"
                     /translation="MFRLLIAIVCVLPCLAVAQSIYKVQLPDGSIMFTDTPPPGAKVL
                     EEREAKSAPRPAARTTTPTTAARQAMPGPLGEPAPASAPKVRPIDAAIQEIEAAERAV
                     QVAKRRLELGREPLPGERLGLAGGGSRLSPDYEARVAGLEREVADAESRLTKAHAARN
                     AAR"
     gene            4109..4684
                     /locus_tag="VLN25_00525"
     CDS             4109..4684
                     /locus_tag="VLN25_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HSQ09093.1"
                     /translation="MATAQERMLEILVGGVVIVVVLAFVAGVTLVVLRARSQTTLPAG
                     RWPFSLRRPLSEPEQVLYYRLCHALPDHLVLAQVGLSRFLAVKKGHDPRAWHNRINRM
                     SVDFLVCNKDASVVAAIELDDASHARPDRVSADEKKERALEAAGVRLLRWSVDAMPDE
                     SAIQVAFEPGSPSRRSRMPTGQPSEAAYRPA"
     gene            4909..5586
                     /locus_tag="VLN25_00530"
     CDS             4909..5586
                     /locus_tag="VLN25_00530"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HSQ09094.1"
                     /translation="MSKRNVVVGALLAAASPFLMGNAAAVPVTWTDWLTISSPIALGN
                     MGGVTVTATATSGNMDGPSQTACGNNWWTEPDPSNPAYTGGSVSNAPTACEQVGLSSP
                     VSITVTFSAPTSNLYMALLSVGQPNLTVTYDFDTAFTIDSEGRGFWGDGSYVLGAGDT
                     LAMNEVHGVLAFAGPISSLTFTTSPSEYWHAFTFGFAATAVPEPGTLAVLSLGLAGLA
                     FARRRRH"
     gene            5865..6821
                     /locus_tag="VLN25_00535"
     CDS             5865..6821
                     /locus_tag="VLN25_00535"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HSQ09095.1"
                     /translation="MASDIFLSYSRADQQLADQFVKIACERGLSVWYDQMIEGGDDWR
                     SKIVDAVSSAKALVILFSDHSNGSRQLIKELAIADSFQKLVIPVLIANCQPRGAYLYE
                     LAARNWVNLHPDPSTRLAPLIENLLKEIGSGDSGSSSAPARANHEHVSPPTSSTQGRP
                     SMVAHVASSTQSARARSGDESWFPLKRYDLYVLVPILAAGFLLGIFGEGDNKSGGLGL
                     SAIAFFSYMLVIAVRNARLNRSILSGKSFASYFAVLAIGSAPALVIEGTTASNVTTFL
                     GLLVISLILAVAANVLQVILRKVFQQKLFRSKIEEPLEARAP"
     gene            complement(7031..7106)
                     /locus_tag="VLN25_00540"
     tRNA            complement(7031..7106)
                     /locus_tag="VLN25_00540"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7070..7072),aa:Glu,seq:ttc)
     gene            complement(7152..7227)
                     /locus_tag="VLN25_00545"
     tRNA            complement(7152..7227)
                     /locus_tag="VLN25_00545"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7192..7194),aa:Ala,seq:ggc)
     gene            complement(7255..>7762)
                     /locus_tag="VLN25_00550"
     CDS             complement(7255..>7762)
                     /locus_tag="VLN25_00550"
                     /inference="COORDINATES: protein motif:HMM:NF039902.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HSQ09096.1"
                     /translation="NGEYMKEADNARLARLAATRIEARGGVLAKGPALERVVALLKDR
                     SRTLNELADAALLFYRDVAREPALLTEHLQGSGHEAVRMFRERAGELTWERAAISGLI
                     KQVVGELKLKMPQVAVPLRVAVTGQTQTPSIDAVLELIGRDAVLARLDTALEQGAGQR
                     GGSIARPI"
BASE COUNT         1408 a         2491 c         2435 g         1428 t
ORIGIN      
        1 cactgccgtt gcccggcacg cgatacgggc tgagcgcctc gcgcaggcgc gccgcgtcac
       61 cggcgccatc cagctcgatc cacagtcggg cggcggcatt ggcgcgcgct tcagtcaggt
      121 ccatcaggtc atcggcagag atcgatagcc gctgcgcgaa ctcgtcgaag cgtaccttgc
      181 ccaccacaca gagcagctgg tcctccttga gcagcgcgcg cttgcgatcg tacagttcgg
      241 aaaacaccat cagctcgagc tgcgcggttc cgtcgtcgag cacgacgact cccatgcggc
      301 cgcgccgcgt gttctggctg cgcgccgaca cgatcacccc ggtgagcctc acctggtcac
      361 gcgcctgccg gacctcgacg agcggcgttg gcgcaagaag tcgcgcctcg gtcgcgtact
      421 cgcggaacag gtgcccggag aagtagtagc caagcgcgag cttttcgttc gtcagccgtt
      481 cgcgttcgct ccacggcgcg gccctgacgt aatcgatgtg ccgcaccgat gcctcgccgc
      541 cgccgaaaag gcttgcctgt ccggcgctgg cggcagcgct atccgctgcc tcgatcgcgc
      601 gcccgaccgt cgcaagcagc gtggcgcgat cgtcgtgcag ccgatcgagt gctccggcgc
      661 gcacgagcgc ctcgaccacg cgccggttga ccacatgctt gtcgacgcgg gcacacagat
      721 cgaacaggct ctcgaacggg ccacctgtcg agcgtgccgc caggatcgcc tcgatcgcgc
      781 cctgcccggt gcccttgatc gcgccaaggc cgtagcgaat tgtttgcata tccaccggct
      841 cgaagcgcca tccaccggca ttgacgtcgg gagcctggac cgtcaacctg agggccttgc
      901 agtcctcgat caggtccttc accttgtcgg tgtcgtcctt caccgcggtc aggttggcgg
      961 ccatgaacgc ggccggatag tgcgctttga agtacgccgt ctgataggcg acaacggcgt
     1021 aggccgccga gtggctcttg ttgaatccgt agccggcgaa cttctccatc aggttgaaga
     1081 gctcggtcgc cgtgttcgct tccacaccct gcttgcccgc accctcgacg aagatcgagc
     1141 gctgctttgc catctcctcg ggcttcttct tgcccatcgc acgccgcagc aggtcggccc
     1201 cgccgagcga atagccgccc accacctgcg cgatgcgcat cacctgttct tgatagacca
     1261 tcacgccata ggtctcggcg aggataggtt cgaccgaagg atgcaggtac tcgacccgct
     1321 gacgtccgtg cttgcgttcg atgaagtccg ggatcaggtc catcggaccc ggccggtata
     1381 gcgcgttgag cgcgatcagg tcctcgaggc ggtcgggctt ggcgcgcttc agcaattcgc
     1441 gcatgccgcg cgattcaaac tggaagatcg ccgccgtcgc cgcctgcttg aatacgtcat
     1501 aggtcttcgc atagtcgagg ggcagtgtgt ccagcctgag attgaaggcc gggtcgagct
     1561 gccgcacgta ctgcagcgtc aggtcgagga tcgtcagcgt cgtcaggccg aggaaatcga
     1621 acttgaccag gccgatcgct tcgacatcgt ccttgtcgaa ctggctgatc gcagcgccct
     1681 gggcacccgg cgcgcagtac agcgggcaga agtccgtcag cttgcccggt gcaatcagca
     1741 cgcctcctgc atgcatgccg acgttgcgcg tcaggccctc gaggggccgc gcgagcgcaa
     1801 tgagttcctg gttttcctcg tcgctgttga cggcctcggc gaacgccggc tcttcacgaa
     1861 gcgcacgatc gagcgtccag ggatcggccg ggttatgcgg gatcagcttc gaaagctggt
     1921 cgcacttcgt gtaaggcaga tcgagcactc ggccggcatc gcgcactacc gctttcgcac
     1981 cgagcgtgcc gaaggtcgca atctgcgaga ccgccgcggc accgtacttc tgcttgacgt
     2041 agtcgatcac gcgatcgcgg ttgtcctggc agaagtcgat gtcgaagtcg ggcatcgaca
     2101 cccgctcggg gttcaggaag cgctcgaaaa gcagcccgta acgcagcgga tcgagatcgg
     2161 tgatgccgag cgaatatgcc accagcgagc cggcgcctga gccccgtccc ggccccaccg
     2221 ggatgccgtt gcccttggcc cagttgatga agtcggcaac gatcaggaag tagccggaaa
     2281 agcccatctt cgcgatcgtc ttcagttcga gatcaagtcg cgccgcgtac tccggtcgcc
     2341 gctgcgcacg gacggtgtca tccgggtaga gcgactgcag ccgctgttcc agaccggtgc
     2401 ccgtgaggtc gcggcaatga tcgtccagcg tgacgccgac gggcgttgga aacggcggca
     2461 gccgcgcctt tccgagttcg agcgtgaggt tgcagcggcg cgcaatctcg acggcattgg
     2521 ccagcgctgc cggaacatcg gcaaacagga cagccatctc ggaccgggtc ttgaagtact
     2581 gctcgcgcgt gaaacgtcgc ggccggcgtg ggtccgcgag cgtgtagccc tcggcgatgc
     2641 agtagcgcgc ttcgtgtgcg cggaagtcgt ccggcacgag gaactggatc gggtgggtgg
     2701 ccacgaccgg cagatgctgc ttgagcgcca gcaccagcgt tgcgtgcgtg caggccgcgt
     2761 cgcgcgcgtc gccggtgcgc tgcagttcga gataaaagcg gcccggaaac aatgccgcca
     2821 gccgctgtgc ggccagaatg gcggcatcgg gcctgctggc catcagatgc gcgccgactt
     2881 cgccgagccg tgcgccggac agcgcaatca gccccgccgc gcacgcgccg gagtcggcct
     2941 cgagccagtc gaggcgcagt tccgcgcggt tgcgatgctg gttctcgagg aaggcccgtg
     3001 tcagcagcgt gcaaaggttc aggtaaccgg accggtcctg cgccagcagc aacaaacgat
     3061 aggggttatc gcggtcgaca tcgttggtga cccagacgtc gcaaccgaga atcggcttga
     3121 cccccttgcg ccgtgcggcc tggtagaagc ggaccgcacc gaagacattg gcactgtccg
     3181 tcagcgccaa agcgccctgg ccatccgccg ctgccttggc cacagcatcg tcgatgcgca
     3241 cgatgccgtc gacaaccgaa aactccgaat gcaggcgaag atggacgaac tgcgtaccgc
     3301 tcatgtcaag gattctgcca cccgcgggtc acgacctctg tggataagct gtcgcgtcag
     3361 cgtattcatg ccctaaggcg ttgatctaaa acgtcggaat gagcggtgct tttccgcccg
     3421 ttggcgatat cgccggtgta tcggaccggt tattgtttcc aacgccctta gggagtgcct
     3481 cgatgtttcg tcttctgatc gccatcgtct gcgtgctgcc gtgcctggcg gtcgcgcaga
     3541 gcatttacaa ggttcagttg cccgacggca gcatcatgtt cacggacacg ccgccgccgg
     3601 gtgccaaggt gctcgaagaa cgagaagcga agtccgcgcc tcgacctgct gcacggacca
     3661 cgacgccgac gaccgcagcc cggcaggcca tgcccggccc attgggtgaa ccggcaccgg
     3721 cgtccgcccc caaagttcgg ccgattgacg cagcaattca ggaaatcgag gccgcggagc
     3781 gcgccgtgca ggtcgccaag cgccgtctcg aactgggccg tgaaccgctt ccgggcgagc
     3841 ggctcggatt ggccggcggc ggcagccgcc tgtcccccga ctatgaagcg cgcgttgcag
     3901 gcctggaacg ggaagtggcc gacgccgagt cgcgcctgac caaggcccac gcagcgagga
     3961 acgccgcgag gtagtttcgc gcgtacgacg tccgccgcgc agcgcactgc gcttcacgac
     4021 gcccgaactt tagggcgatt tcaggagcgg aagccgggct tcatcaggcg tttgccggac
     4081 cctaacgccg gctacctttg cggccgtcat ggcaaccgct caggaacgca tgctggaaat
     4141 tctcgtgggt ggggtcgtaa tcgtcgtggt cctggcgttt gtcgccggcg tcacgctggt
     4201 cgtgctgcgt gcgcgatccc aaaccacact tcccgccggt cgctggccct tcagtctgcg
     4261 caggcccctg tcggagcccg agcaagtgct gtactaccgg ttgtgccacg cactgcctga
     4321 ccacctggtg ctcgcgcagg tcggcctctc acgctttctg gcggtaaaaa aaggccacga
     4381 tccacgcgca tggcacaacc gcatcaaccg catgagtgtc gacttcctgg tgtgcaacaa
     4441 ggacgcgagc gttgttgcag cgatcgaact cgacgacgca tcgcacgcgc gtccggaccg
     4501 tgtcagcgcc gacgagaaga aggaacgagc gctggaggcc gcgggcgttc gcctgctgcg
     4561 ctggagcgtc gacgcaatgc ccgatgagtc tgcgatacag gtggcgtttg agcccgggtc
     4621 gcccagccgc agaagccgga tgccaaccgg tcaaccatcc gaagccgctt ataggcctgc
     4681 atgagttggt cctcaacacc gccggagtcg gcttctctgt tctagacacc ggggtcctcc
     4741 gtcgagcgac tgatgtgtgg gaggcatgaa ccggaggatc cgaaatcgcg tcggaattaa
     4801 ttacagtcac tctccccgtg cgtggccgtg agcccctaag gtattgattc ttataggttg
     4861 gcgcgacatg tgcttgcaaa gagcatggtt gatcacgaag ggatgaccat gtcgaaacgc
     4921 aatgtcgtag tgggtgcctt gctggcggcc gcgagcccgt tcctcatggg caatgctgcg
     4981 gcagtccccg tcacctggac ggactggctc acgatctcca gtcccatcgc gttgggcaac
     5041 atgggcggag tgaccgtcac cgcgacagcg accagcggca acatggacgg cccttcgcag
     5101 accgcctgcg gaaacaactg gtggacggaa cccgatccca gcaatccggc ctatacgggc
     5161 ggcagtgtga gcaatgcgcc gacggcctgc gaacaggttg gcctgagctc acccgtgtcg
     5221 atcacggtaa cgttctcggc gccgacgagc aatctctaca tggcgttact cagcgttgga
     5281 cagccgaatc tcacggtcac ctacgacttc gacacggctt tcacgatcga tagcgaaggc
     5341 cgcggcttct ggggcgacgg cagctacgtg ctgggtgcag gcgacacact ggcgatgaac
     5401 gaagtccatg gcgtgctggc cttcgccggc ccgatctcgt cactgacttt cacgacctcg
     5461 cccagcgagt actggcacgc cttcacgttc ggcttcgccg ccacggcggt tccggagccg
     5521 ggcaccctgg cggtgctgag ccttggcctc gcgggcctgg cgtttgcgcg tcggcgcagg
     5581 cactgatcag ctcgctgcgc aaagaacaga cccgcccacg tggcgggttt tttctttctg
     5641 cggctccgca gacatcgtca agactggcct gcgtcatggg gaacaactat cgaccggctc
     5701 gagtcgagcg ttcgaatcct ttctggatgc aagagccggg agaacctgac cggcatcgct
     5761 ggcaggacta ggatgaatgt cggtcgcggg tccaagagcg gttcgcctga gacctctcga
     5821 attgcaaacg gcgcgaaagg cttctcaatc gggcgcaaac gcatatggcc agcgatatct
     5881 tcctgagcta cagccgcgcc gatcaacagc tcgcggatca gttcgtcaag attgcatgcg
     5941 agcgaggact gagcgtctgg tacgaccaaa tgatcgaagg aggggacgac tggcggtcga
     6001 agatcgtcga tgctgtgagc tccgccaaag ccctggtgat tctcttttca gatcactcca
     6061 atggctcgcg ccaactcatc aaagagctgg cgatcgccga cagctttcaa aagctcgtaa
     6121 tcccggtcct gatagccaac tgccagccgc gaggtgccta tctttacgaa ctggccgcgc
     6181 gcaattgggt caacctacat cccgatccga gtacgcgcct ggcgccgctc attgaaaacc
     6241 tcctgaagga gatcgggtcg ggagactccg gttcttcatc cgccccggcc cgcgccaatc
     6301 atgagcatgt gagcccaccc acgagcagca ctcaggggag accgtcgatg gtcgcacacg
     6361 tcgcgtcctc gacgcaatct gcacgggcga gaagtggcga cgagagttgg ttcccgctga
     6421 aacgatacga cctgtacgtt ctcgtgccga tactggccgc cgggttcttg ctgggaattt
     6481 tcggtgaggg cgacaacaag agcggtggat tgggattgag tgcgattgca ttcttcagct
     6541 acatgctcgt catcgccgta cgaaacgccc gattgaaccg gagcatcctt tccggcaagt
     6601 cgttcgcttc ctactttgcc gtgctggcga tcggaagcgc acctgcactc gtaattgaag
     6661 gaacaacggc aagcaacgtg acgacctttc tcggattgct ggtcatctcg ctcatcctgg
     6721 ccgtcgctgc caacgtgctg caggtcatcc tgcgcaaagt ctttcagcag aaactctttc
     6781 ggagcaagat cgaagaaccg ctggaggcac gagcgccgtg atctggacat cgcgtgaggt
     6841 ccatcgcgcg agttcacggt catgcaaatt cgcgaacggg tgaaacgccg atggcagcgc
     6901 gtcgcgagtt cctgccgggc cgcaccttcg acctcggcca ttcaagatgg ttttcgcgat
     6961 gccttctcga cacacgtaag actagacgca aagaaaaagg ggttatcttc cgataacccc
     7021 ttgatttgac tggcgtcccc tacgagattc gaactcgtgt tatcgccgtg aaagggcggt
     7081 gtcctaggcc tctagacgaa ggggaccggt tcaattcaaa gcccgcatga gcgggctttg
     7141 gaaacggtct ttggtggagg taagcgggat cgaaccgctg acctcttgca tgccatgcaa
     7201 gcgctctccc agctgagcta tacccccacg gagagccgcg gagtatagcc gttttcagat
     7261 cggcctcgca atcgagccgc ccctttgtcc cgcgccctgc tcgagtgcag tgtcgagccg
     7321 agccaacacg gcgtcgcgtc cgatcagttc aagtacggca tcgatcgacg gcgtctgcgt
     7381 ctgtccggtg acggccacac gtaacggcac cgcgacctgc ggcatcttga gcttcagttc
     7441 gccgacgacc tgcttgatga ggccgctgat ggcggctcgt tcccaggtca gttcaccggc
     7501 gcgctcgcgg aacatccgga ccgcctcgtg gccagagccc tgcaggtgtt cggtgagcag
     7561 tgccggttcg cgtgcgacgt cccgataaaa cagcagtgcg gcgtcagcca gttcgttcag
     7621 ggtccgcgat cgatccttca gcagggcgac cacgcgttcg agtgccgggc ccttcgcgag
     7681 cactccgccg cgcgcttcga tacgcgtcgc agcaaggcgc gccagccgtg cgttgtcggc
     7741 ttctttcatg tactcgccgt tc
//
LOCUS       DATIRT010000024         2558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1084429, whole genome shotgun sequence.
ACCESSION   DATIRT010000024 DATIRT010000000
VERSION     DATIRT010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084429"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..812
                     /locus_tag="VLN25_00555"
     CDS             234..812
                     /locus_tag="VLN25_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09097.1"
                     /translation="MDRRTSFLAQDPDERTVRWGWVFWIAWAAVGGALLLGDYRGGAI
                     GIGAVLAAPFWALWALWAAYRGLRAYLRWQGAAAWGVANGQHYEFDGRRVRVTFDGDN
                     IWFAASDVFDVLSTQPGARDPERVRLIVGREGLTELPATGVLSFSEKGLAAWLDRRTD
                     RSAGQFTRWIDTQVIAPYRRRLELEKVIDSKT"
     gene            881..1492
                     /locus_tag="VLN25_00560"
     CDS             881..1492
                     /locus_tag="VLN25_00560"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HSQ09098.1"
                     /translation="MSAVARVFSEHRIGVSRIFGFAFAAAVLFTGSRWQGTLAADLMF
                     LVGLMLAAVGMVGRIWCLVYSSGYKSSQLVTAGPYVISRNPLYFFSLVGLVGIGLSTA
                     TVTLTLFVILFFWAVYPSVIHGEEAFLREKFGASYTEYCDRTPRFFPSWGLLTEPDHY
                     EVRPKTLRQSLGGVLWFIGLPALIHLLMELRELGIIGWVALLP"
     gene            complement(1529..2314)
                     /gene="xth"
                     /locus_tag="VLN25_00565"
     CDS             complement(1529..2314)
                     /gene="xth"
                     /locus_tag="VLN25_00565"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HSQ09099.1"
                     /translation="MPLTLATWNVNSLKVRLNHLLEWLATHRVDIVCLQETKLIDENF
                     PVAALADAGYDAVFAGQKTYNGVAVLTRRATMPAAVDVVTALPGFEDPQKRLIAATVG
                     PLRVVGAYFPNGQSVGSDKYDYKLRWIAALNTWLRDALARHPMLALAGDFNIAPEERD
                     VHDPQAWAGQVLVSEPERQAFRDLLALGFVDSFRLFEQPERSYTWWDYRMMAFRRKMG
                     LRIDHVLLASSLAPHCTACSIDVAPRRLEQPSDHAPVIVTLSL"
BASE COUNT          395 a          793 c          873 g          497 t
ORIGIN      
        1 aaggcgcggc ggtccttgcg caccagccgg cagacaaggc gtgacgagtt cgctggaaga
       61 ttctggcaag gctggcgaca gggctgtcgt tcgcgtcggg cgaccgcgag cagcgtcgcc
      121 tgcgtgaact gatcgacggg gaacagctcg gggctgaccg gggcagcatt ggtgctccga
      181 tagcccgggt cggtgccgca cgggcgcgtc cggccctcgg gtaccctagt gccgtggatc
      241 gtcgtacttc cttcctcgct caggatcccg acgaacgaac cgttcgctgg gggtgggtgt
      301 tctggattgc ctgggccgcc gtgggcggcg ccctgctgct gggggactat cgcggcgggg
      361 cgatcggcat cggggccgtg ctggccgcac cgttctgggc gctgtgggcc ctgtgggctg
      421 cctatcgcgg ccttcgcgct tatctgcgtt ggcaaggtgc tgcggcctgg ggagtcgcga
      481 acggccagca ctacgagttt gacggccgtc gcgtccgagt cactttcgat ggcgacaaca
      541 tctggttcgc agcgtccgac gtgtttgacg tgctgagcac acagcctggc gcacgcgatc
      601 cggagcgggt acgactcatc gtaggccgcg agggcctgac cgagctgccg gcgaccggtg
      661 tgctcagctt ctccgagaaa ggcctggcag cctggcttga tcgccgcacc gatcgatcgg
      721 ccggtcaatt cacgcgctgg atcgacacgc aggtcattgc gccgtatcgc cggcgcctcg
      781 agctggaaaa ggtgatcgac tcgaagactt aacggcccat aacctcggcc ggacggtccg
      841 ggagataatc cgggcagctt cgcataacgg aattttctgc atgtcggctg tcgcccgtgt
      901 cttctccgag caccgtatcg gtgtgtcccg aatcttcggg ttcgcgtttg ccgctgcggt
      961 gttgttcacg ggcagtcgct ggcagggcac ccttgctgcg gacctgatgt ttctcgttgg
     1021 cctgatgctc gccgctgtcg gcatggtcgg ccgcatctgg tgcctggtgt actcgagcgg
     1081 ttacaagagt tcccagctgg tgacggccgg cccctacgtc atttcgcgca atccgttgta
     1141 tttcttttct cttgtaggcc tcgtgggcat cgggctttcg acggcgacag tgacgctgac
     1201 gctcttcgtc attttgtttt tctgggcggt ctatccgtcg gtcatccatg gagaggaagc
     1261 tttcctgcgc gagaagttcg gggcgagtta caccgagtac tgcgaccgca cgccacgctt
     1321 cttcccgagc tgggggctgc tgaccgagcc agaccactac gaagtacgac ccaagacgct
     1381 tcggcaatcg ttgggaggcg tgctgtggtt catcggcctg ccggcgctga tccatctact
     1441 gatggaattg cgcgaactcg gcatcatcgg ctgggtggcg ctgctgccct agtcctatcc
     1501 ccatccgcgc cgggcatccc ggaacgcgtc agagcgacag cgtcacgatc accggtgcgt
     1561 ggtcgctcgg ctgctcgagc cggcgaggtg ccacatcgat gctgcaggcg gtgcagtgcg
     1621 gcgccagtga cgacgccagc agcacgtggt cgatgcgcag gcccatcttg cggcggaacg
     1681 ccatcatccg gtagtcccac cacgtatagc tgcgctcggg ctgttcgaac aggcggaagc
     1741 tgtcgacgaa gccgagtgcc agcaggtcgc ggaaggcctg tctctccggc tcggagacga
     1801 gcacctgccc ggcccaggcc tgcggatcgt ggacatcgcg ctcctccgga gcaatgttga
     1861 aatcgccggc gagtgccagc atcggatggc gcgcgagggc gtcgcgcagc caggtattca
     1921 atgcggcgat ccagcgcagc ttgtagtcgt acttgtcgct gccgacgctc tgcccgttgg
     1981 gaaagtaggc tccgaccaca cgcagcggac cgaccgtcgc tgcgatcagg cgcttctgcg
     2041 gatcctcgaa accgggcagc gcggtgacga catcgacggc cgcgggcatc gtcgcccgtc
     2101 gcgtcagcac ggccacaccg ttgtaggtct tctggccggc gaagacggcg tcgtagccgg
     2161 cgtccgccaa tgcggccacc ggaaagttct cgtcgatgag cttggtttcc tgcaggcaga
     2221 caatgtcaac gcggtgcgtc gcgagccatt cgagcaggtg gttcagtcgg accttgagcg
     2281 aattgacgtt ccaggtcgca agggtgagtg gcatggtgaa cctgtctcgg agtccccgac
     2341 ggatcgcgtt gcgaaaggga gcgggcggat gctaggcggc gcgcgatgga cagtagcgtg
     2401 ctacggccga gcgcggcaac aacgcagacg cctgctctct ttcgcaaccc tgcggggcgg
     2461 ggtcgcgcat tcgcaaaatg agggcgcatg gcgttgttgc ggctcgggtc cttgcacacg
     2521 caatggtccc tcgccgcgcc tagccctgcg cccaatgc
//
LOCUS       DATIRT010000025         1816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_691263, whole genome shotgun sequence.
ACCESSION   DATIRT010000025 DATIRT010000000
VERSION     DATIRT010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691263"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VLN25_00570"
     CDS             complement(<1..192)
                     /locus_tag="VLN25_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09100.1"
                     /translation="MAHLAGVKSAYRDWTRAPERGSPGLLALIVRLARWFPREPVSLL
                     LYPIVAYFVTFSPRQVAASR"
     gene            complement(171..473)
                     /locus_tag="VLN25_00575"
     CDS             complement(171..473)
                     /locus_tag="VLN25_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09101.1"
                     /translation="MSAESLSARYCVDAGHRALAGHFPGDPIVPAVVLLRFVELTLAR
                     AGQRLTGIERMKFLRPVRPGEHIDVTVVPIDSSRGSVEMAVEGGIVARGLWHTSQE"
     gene            complement(463..>1816)
                     /locus_tag="VLN25_00580"
     CDS             complement(463..>1816)
                     /locus_tag="VLN25_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HSQ09102.1"
                     /translation="DQSRTLIVWSDAQCSVGALQAVAFQLSRSLPPGRRVLVECEDRA
                     VFLWAVLAVWAARRTVVMPPPDLKATPGDSPRSHFDCIVTDRQEQDSSTDLPTLRVDA
                     AALRAALEAHPVVATHIALPMSHVAAVFFTSGSTGQPTAQPKTWRQLVEAADAMGDLL
                     QLGDAAVLLGATVVHSHMFGFEMLVMQALRGPGVVYASRIVFPSDLALFAAVNAPRKW
                     LVTTPYHLGLFVEAGPLPAGLQRVVAATMPLDAALAAKVEAGTGAQVHEIYGSTEAGC
                     IATRRPVASPVWRLASDLRLSVKNDGKAVLAGRRVGGMLEVRDCIVLSDNGFELLGRD
                     TDIVKVAGKRTSLQALTAVLRGIQGVEDGSFIDGAAIGQKRLVAIVVAPGLSSERIRE
                     ALAARIDAAFLPRPLVLVDSMPRDANGKVRLDALVQSAAAARRKTTPPSTEVLRSDVS
                     "
BASE COUNT          345 a          624 c          585 g          262 t
ORIGIN      
        1 acgcgacgcc gcgacttgcc gcggtgagaa ggtgacaaag taagcgacga tcggataaag
       61 cagcagcgag accggttcac gcgggaacca ccgtgccagc cggacgatga gtgccaacaa
      121 accgggacta ccgcgctcgg gcgcgcgagt ccagtcgcgg taggcgctct tcactcctgc
      181 gaggtgtgcc ataggccccg agcgacgatt ccaccctcga ccgccatttc caccgagcca
      241 cgactgctgt cgatcggcac gaccgtgacg tcgatgtgtt ctccgggtcg caccggtcgc
      301 aggaacttca tgcgctcaat gccggtgagc cgttgaccgg cacgcgcgag cgtcagctca
      361 acgaatcgaa gcaatacaac cgccggaacg atcgggtcgc cgggaaagtg acccgccagc
      421 gcacgatggc ccgcgtcgac gcagtaccgg gccgaaagcg actcagctga catcggagcg
      481 cagcacctcg gtcgatggtg gggtcgtctt ccggcgcgcg gcggcagccg actgaaccag
      541 cgcgtcaagc cgcactttgc cattggcgtc gcgcggcatg ctgtcgacca gcaccagagg
      601 gcgtgggagg aatgcggcat cgatgcgcgc cgccagtgcc tcgcgtatcc gttcgctcga
      661 cagtccgggc gcgacgacga tggccaccag acgcttctgt ccgatcgcag cgccgtcgat
      721 gaaagagcca tcttcgacgc cctggatgcc gcggagcaca gccgtcagcg cctgcagcga
      781 agtgcgcttg ccggcgactt tcacgatgtc ggtgtcgcga ccaagcagtt cgaacccgtt
      841 gtcgctcagc acgatgcagt cgcgcacttc gagcatgcca ccgacacgac ggcccgccag
      901 caccgccttc ccatcgttct tcacggacag tcgcaagtcg ctggccagcc gccacaccgg
      961 gctcgccacc ggacgacgcg tggcgatgca acccgcctcg gtactgccgt agatctcgtg
     1021 cacctgcgca ccggtgccgg cttcgacctt ggcagccagc gcggcatcca acggcattgt
     1081 cgccgcaacg acgcgttgca gtcctgcggg caatgggccc gcctcgacga acagccccag
     1141 gtgatacggc gtcgtcacca gccacttccg gggcgcattg actgcagcaa agagggccaa
     1201 gtcgctcgga aagacgatcc gcgatgcata gacaactccc gggccgcgga gcgcctgcat
     1261 caccagcatc tcgaagccga acatgtgcga gtgaacgacc gtagccccga gcagcacggc
     1321 tgcatcaccc aactgcaaga gatcgcccat cgcgtcggcc gcttcgacga gctggcgcca
     1381 ggtcttgggc tgagcggtcg gctgtccggt gctgccggac gtgaagaaca ccgcagcaac
     1441 atgcgacatc ggcaaggcga tgtgggttgc cacgaccggg tgcgcctcaa gcgctgcccg
     1501 aagagcggcg gcgtcgacgc gaagcgtcgg cagatcggtc gagctgtcct gctcctggcg
     1561 atccgtgacg atgcagtcga aatgggatct aggcgaatcg cccggcgtgg ccttgaggtc
     1621 gggcggcggc atcaccacag tgcgtcgggc tgcccagacg gccagcacgg cccacaggaa
     1681 caccgcgcgg tcttcgcact ccaccagcac gcgtctaccc ggcggcagtg accgcgaaag
     1741 ctggaaggcg accgcctgca gagcgcccac tgaacactgg gcgtcgctcc agacgatcag
     1801 cgtgcggctc tggtcc
//
LOCUS       DATIRT010000026         2519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_199724, whole genome shotgun sequence.
ACCESSION   DATIRT010000026 DATIRT010000000
VERSION     DATIRT010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199724"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            465..1907
                     /locus_tag="VLN25_00585"
     CDS             465..1907
                     /locus_tag="VLN25_00585"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSQ09103.1"
                     /translation="MVFSFFKKDPKDAEPSSGKGGASRAAKPAARPLAGPVNRNAAAT
                     QPKPLNTDTSLPDKDLARTLAMETAAKIDAIESEMARDFMRPSGGGPVFSNSAANSTL
                     GRLSQQPVEQPLPPQTAVKRDPGMELLDLSVDEWQGNANAIELHGEGSGSAMDEAAIL
                     FANGLIEPAEAGLRAAIHSDSLGDAAQRAWLMLFELFQQRGDKGSFDNLTIEYVLRFE
                     SSPPAWIDYKDDAAILAAGKTADGAPIVRLPEAVDANVVKVLEQLKNHSAQFQSLTLD
                     TSGVREIDLVGAELLLRVISAFKRASHELLVLGADQLITPLRSAVEPGRRDQSDAAWM
                     LLLEVFRLLQRQHDFEETGIQYCITYEVSPPSWEPPPPSLKTRAATPADKPVEVEDGM
                     QWRGTVRANGEPQFGRLLTAAKMEKRLAIDCTYLKRIEFSTATALLSLLTKLRQGGVT
                     VEFVNLNHMIAALFSLLGVDMVADIQLRRV"
     gene            1948..2484
                     /gene="hslV"
                     /locus_tag="VLN25_00590"
     CDS             1948..2484
                     /gene="hslV"
                     /locus_tag="VLN25_00590"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HSQ09104.1"
                     /translation="MEPFHGTTIVSVRRGAAVAMGGDGQVTLGNIVVKQTARKVRRLY
                     HDRILAGFAGATADAFTLFERFEGKLEKHQGHLVRSAVELAKDWRTDRILRRLEAMLA
                     VADHEASLIITGNGDVLEPELGMIAIGSGGPYAQAAARALLDHTDFPPADIVKKALTI
                     AGDLCIYTNQSHTIESLD"
BASE COUNT          462 a          817 c          806 g          434 t
ORIGIN      
        1 ttctgcctgt cgctcggccc cgagtacatc gtcgaagtga aatcgaaagc ggcgatcaag
       61 cacacctacg agccgatggt gctggcgggc cgtctgaacg tgctgaccga ggaccccttc
      121 ggtctgtatt accgccttag cgatgcccag ctggcgaccg cggaaaagta atccggccgg
      181 ctggttgcgc ggtagccgac aggcgggata gtcgcccaga tcaacggttt gcccgggaac
      241 gaaagttgtt actatgggac gaaccttcgg ctcggacgtg ccggcgttac ctatcggtcg
      301 cccgatcgtg aatccggtcc ttgcccgcgg gcgtcttgtc cgaatgctcg cgcgtatggg
      361 gtatcgggtc tgactcgtgt tgaggccgtg cctgccgtag tttgagatac aaggttcagg
      421 ggcgcagtag cgacagttcc ggactaacaa caccagaagg cagcgtggtc ttctcgttct
      481 tcaagaagga tcccaaggac gccgagccgt cgtccggcaa gggcggcgcg agtcgtgcgg
      541 ccaagccggc cgcgcgtccg ctcgccggtc cggtgaatcg caacgccgcg gcgacccagc
      601 cgaagccgct gaataccgac acttcgctgc ccgacaagga cctcgcccgc acgctggcga
      661 tggaaacggc ggcgaagatc gacgcgatcg aatcggaaat ggcgcgtgat ttcatgcgcc
      721 cgtccggagg cggccccgta ttctcgaatt cggcggccaa ttcgacgctc ggtcgcctgt
      781 cgcagcagcc ggtagagcag cccctgccgc cgcagacggc ggtcaagcgc gatcccggta
      841 tggagctgct cgatctttcc gtcgacgagt ggcaaggcaa cgccaacgcc atcgaacttc
      901 acggcgaagg gtcgggctcg gcaatggacg aggcggcgat cctgtttgcc aacgggctca
      961 tcgaaccggc cgaagccggg ctgcgcgccg ccattcactc ggactcgctc ggcgatgcgg
     1021 cccagcgggc gtggctgatg ctgttcgaac tcttccagca gcggggcgac aagggcagtt
     1081 tcgacaacct gacgatcgag tacgtgctgc gtttcgagag ctcgccgccc gcatggatcg
     1141 attacaagga cgatgcagcc atcctcgccg ccggcaagac ggcggacggt gcgccgatcg
     1201 ttcgattgcc ggaagcggtg gacgcgaacg tggtcaaggt gctggagcag ctgaagaacc
     1261 attcggccca attccagtcg ctcacactcg atacctccgg cgtacgagag atcgacctgg
     1321 tgggtgcgga attgctgcta agggtcatca gtgcattcaa gcgggccagc cacgaactgc
     1381 tggtgctcgg ggccgaccag ctgatcacgc cgctgcgctc cgccgtcgag ccgggccgcc
     1441 gtgaccagag cgatgcggcg tggatgctgc tgctcgaagt gttccgcctg ctgcagcgcc
     1501 agcatgactt cgaggaaacc ggcatccagt actgcatcac gtacgaagtc tcgccgccat
     1561 cctgggaacc gccgccgccg agcctgaaga cgcgcgctgc cacgccggcc gacaagccgg
     1621 tggaggtcga ggacggcatg caatggcggg gtaccgtgcg tgccaacggc gagccgcagt
     1681 ttggtcgtct tttgaccgcg gccaagatgg aaaagcgcct ggcgatcgac tgcacctacc
     1741 tcaagcgcat cgagttttcc accgccaccg cactgctgtc gctgctgacc aagctgcgcc
     1801 aaggcggggt cacggtggag ttcgtcaacc tcaatcacat gatcgccgcg ctgttttcgc
     1861 tgctcggcgt cgacatggtc gccgacatcc agttgcgtcg ggtctgatcg tccccaagta
     1921 ggccgtaact ttcggccgga tctctccatg gaaccctttc acggaacgac catcgtgtcc
     1981 gtacgccgtg gcgctgccgt cgccatgggc ggcgacgggc aggtcacgct cggcaatatc
     2041 gtggtcaagc agacagcacg caaggtgcgg cgtctttatc acgaccgcat cctggcgggc
     2101 ttcgccggcg cgaccgcaga cgctttcacg ctgttcgagc gcttcgaggg caagctcgaa
     2161 aagcaccagg gtcacctggt gcgctcggcg gtggaactcg ccaaggactg gcgcacggac
     2221 cgcatcctgc gtcgtctcga ggcgatgctg gcggtcgcgg atcacgaggc ctcgctgatc
     2281 atcaccggca acggtgacgt gctggaaccg gaactcggca tgatcgccat cggttcgggc
     2341 ggtccctacg cccaggccgc cgcccgtgca ctgctcgacc acaccgactt tccgcccgcc
     2401 gacatcgtca agaaagcgct cacgatcgcc ggtgacctgt gcatctatac caaccagagc
     2461 cacaccatcg aatcgctgga ctgaccggca acacgccccg tcagctgatg cgtcagcca
//
LOCUS       DATIRT010000027         3515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1478035, whole genome shotgun sequence.
ACCESSION   DATIRT010000027 DATIRT010000000
VERSION     DATIRT010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478035"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1602
                     /locus_tag="VLN25_00595"
     CDS             <1..1602
                     /locus_tag="VLN25_00595"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HSQ09105.1"
                     /translation="KDGVRFSKFYTHASCSPTRATLLSGVDTHLNGLGNMDEWTAPNQ
                     RGAVGYEGYLNNRVATLPQLLRGAGYHTYMVGKWHLGKAPEQIPAARGFERDFSLLDG
                     AGSYWDMTNFTAASPRSVFTEDGRYLTELPKDYYATKTYTDKLIGFIDANRGDGKPFF
                     AYVAHQAPHDPYHLPKEWRSRHVGEYDKGWDAVRQQRLKRQVELGIMPAGTQLAERMW
                     FVPDPIVLAPASRAVLGKKMELYAGMVENLDFHIGRLIDHLKKIGEYENTIFVVFGDN
                     GAEGTDLFKMIAGTPGTRDYLFAAINWSQTHPNAWGDPGSYVGYGPMWAQVSMTPFSQ
                     YKGWLAEGGIRNALIVSGPGVARTKGSLNQGLMHVADLMPTLLEIAGTRYPATLDGRE
                     LPALMGKSWGPMLAGKVDSPRTGQDFLAWELFGNRALRQGDWKLRWQYKPYGKAAWEL
                     FNLATDPVERKDLAAENPDKVRALLTLWDGYVKANNVVLPSRSTFETLEDQLPPRTPD
                     DPGYPPLIYKRQFVPPKELLADPKP"
     gene            1644..2642
                     /locus_tag="VLN25_00600"
     CDS             1644..2642
                     /locus_tag="VLN25_00600"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HSQ09106.1"
                     /translation="MNNDARLTKPVAWARWMVATIFATGLLGGTQALAQVPARFYWKS
                     LSDGNAVPLIINSISGNTNPFDPALTVTPGANFDATMAIMGYAHTFSLFDRSAMAAIL
                     VPMGRVSGEVTVAGRTFDQTSSGFGDPMLEFNLNILGPKAQKNIPDALRYEPGFSVDL
                     LADLALPIGEYDSNQPLNLGQNRWYGRLGMPVVWQLGDWVPGRRTTLEFLPAVWLFGN
                     NTDYVGQTLKTDPMFQLDAHLTRDFTEHLWGSLDMAWYSGGKATINGVAGEKLNNVGV
                     GLTLGYQVNSNLGLTFGYKSTINDNGPGDLKMDGFMISLVYGWHPLIEGSRRLKSE"
     gene            2678..3172
                     /locus_tag="VLN25_00605"
     CDS             2678..3172
                     /locus_tag="VLN25_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09107.1"
                     /translation="MLKTSALLLAIAAGYFVGAANAAVVYCTAPGIPAGCVVRTAAPA
                     AAATAATAKAIYCRSPGVPVGCVARPAARAAAVTPGVGAPGRGAVDPGINQPGALGNR
                     GGGAPGVGVVDPGVNQPGATGNRGGAAVHPGVGAPEVGVNNVGTNGPGVANQNGGVNR
                     AGRR"
BASE COUNT          659 a         1132 c         1147 g          577 t
ORIGIN      
        1 aaagacggcg tgcgcttctc caaattctat acgcacgcca gctgctcgcc cacacgcgcc
       61 actctgctga gcggtgtcga cacgcatttg aacggcttgg gcaacatgga cgagtggacc
      121 gcccccaacc agcggggcgc ggtgggctac gagggatacc tcaacaaccg tgtggccacg
      181 ttgccgcagc tgctccgggg tgccggctat cacacctaca tggtcggcaa gtggcatctg
      241 ggcaaagcgc ccgagcagat cccggccgcc cgcggattcg agcgggattt ctcgctgctc
      301 gacggcgcgg gcagctattg ggacatgacg aatttcaccg ctgcatcgcc gcggtcggtc
      361 ttcaccgagg acgggcgcta tctgacggaa ctgcccaagg actattacgc cacgaagacc
      421 tacaccgaca agctgatcgg attcatcgac gccaatcgtg gcgatggcaa gcccttcttc
      481 gcttacgtgg cccatcaggc gccgcacgac ccctaccacc tgccgaagga gtggcgctcg
      541 cgccacgtcg gcgagtatga caagggctgg gacgccgtgc ggcagcaacg actgaagcga
      601 caggtcgaac tcggcatcat gcccgcaggc acgcagctcg ccgagcgcat gtggttcgtg
      661 cccgacccca tcgtgctggc gccggcgtcg agggcagtcc tcggcaagaa aatggagctc
      721 tacgcgggca tggtggagaa cctcgacttc cacatcgggc gcctcatcga ccacctgaag
      781 aaaatcggcg agtacgagaa cacgatcttc gtcgtgttcg gtgacaacgg cgccgagggc
      841 accgacctct tcaagatgat cgccggcacg cccggcacgc gcgactacct cttcgcggcg
      901 atcaactggt cgcagaccca tcccaatgca tggggcgacc cgggctccta cgtcgggtac
      961 ggcccgatgt gggcgcaggt gtcgatgacg ccgttcagcc agtacaaggg ttggctggcc
     1021 gaaggcggga tccgcaacgc gctcatcgtc agtggaccgg gcgtcgcgcg gaccaaaggg
     1081 agcctcaacc aggggctcat gcatgtggcc gacctcatgc cgaccctgct ggagatcgcg
     1141 ggcacgcgct atccggcaac gctggacggg cgcgaactcc ctgcactcat gggcaagtcc
     1201 tggggcccga tgctggcggg caaggtggat tcgccacgga cgggacagga ttttctcgcg
     1261 tgggaactct tcggcaaccg ggcgctgcgc cagggcgact ggaagctgcg ctggcagtac
     1321 aaaccctacg gcaaggcagc ctgggaactc ttcaaccttg cgaccgatcc cgtcgagcgc
     1381 aaggatctcg ccgccgaaaa cccggacaag gtgcgggcgc tcttgacgct gtgggacggc
     1441 tatgtgaagg ccaacaacgt ggtgctgccc agtcgctcca cgttcgagac gctggaagac
     1501 cagctgccgc cacgcacgcc ggacgatccc ggctacccgc cgctcatcta caagcgacag
     1561 ttcgtgccac ccaaggaatt gctggccgac ccgaagccct gagttccggc gatccgaaat
     1621 ttcatcaacg aggacgacga atcatgaaca acgacgcaag acttacgaag ccggtggcct
     1681 gggcgcgatg gatggttgca acgatcttcg cgacgggctt gctcggcggg acgcaggcct
     1741 tggcgcaggt cccggctcgc ttctattgga agtcgctgtc ggacggcaat gcggtgccgc
     1801 tgatcatcaa ctcgatcagc ggcaacacga acccgttcga tccggcgctc accgtgacgc
     1861 cgggtgcgaa cttcgacgcg acgatggcga tcatggggta cgcgcacacg ttttccctgt
     1921 tcgatcgttc ggccatggcg gcgatccttg tgccgatggg ccgcgtgtcg ggcgaggtga
     1981 ccgtggcggg caggaccttc gatcagacca gcagcgggtt cggcgatccg atgctcgagt
     2041 tcaacctcaa catcctgggc ccgaaggccc agaagaacat tcccgacgcc ttgcgctacg
     2101 agccggggtt ctcggtcgac ctgctcgccg accttgcgct gccgatcggc gagtacgaca
     2161 gcaaccagcc gctgaacctc gggcagaacc gctggtacgg ccgcctgggc atgcccgtcg
     2221 tctggcagct cggcgattgg gtgccgggca ggcgcacgac gctcgagttc ctccctgccg
     2281 tgtggctctt cggcaacaac accgactatg tcgggcagac gctgaagacc gatccgatgt
     2341 tccagctgga cgcgcacttg acgcgcgact tcaccgagca tctatggggg tcgctcgaca
     2401 tggcctggta cagcggtggg aaggcgacca tcaatggcgt cgccggggag aaactcaaca
     2461 acgtgggcgt cgggctgacg ctcggctacc aggtcaacag caacctgggt ctcaccttcg
     2521 gctacaagtc gaccatcaat gacaacggcc ccggcgacct gaagatggac ggcttcatga
     2581 tctcgctcgt ctacgggtgg cacccgctca tcgaaggttc gcgccgcctg aagagcgaat
     2641 gaccggtgtc gtgcccaatc atgaaaggag tacagaaatg ctgaagacat ctgcgttgct
     2701 gctggctatt gccgcaggtt attttgttgg agccgccaac gccgccgtgg tttactgcac
     2761 tgcaccgggc atccccgcag gctgcgtggt acgcactgcg gcaccggctg cggccgcgac
     2821 cgcggcgacc gccaaggcta tctactgcag gtcccccgga gttcccgtag gctgcgttgc
     2881 gcgcccggca gcgagggccg ccgcggtgac gcctggcgtg ggggcgccgg gtcgcggtgc
     2941 ggtcgacccc ggcatcaacc agccgggcgc cctgggaaac cgcggtggcg gtgcgcccgg
     3001 cgtcggcgtt gtggacccag gagtcaatca gcccggagcc acgggaaatc gcggcggtgc
     3061 tgctgtgcat cccggcgtgg gcgccccaga ggttggcgtc aacaacgtcg gcaccaacgg
     3121 gcctggcgtc gccaaccaga acggcggcgt gaatcgagct ggacgacgtt gatctgaaca
     3181 acgctgcggc gccggtcgag accgcgcgaa gtaccgagag cgggtaaacg gagcgtcaat
     3241 cggtcacgct ccgcattgct ttctcacccg tgcgcgtcag cacctgaatc caggatcaaa
     3301 tctgcgccac ctcgcgaaga ggttcgccct tggcaccgct gtagggctgg ccgttgattg
     3361 agcttgatgc atggccgctc tgaaggaaat tgggcggcag cgccgacggg ccggaaatgg
     3421 ccgatagctc cagttgaccg gcgtcagccc aatgactgca atcgcggcat tgctgccgtt
     3481 ggcagcgtga ccctcgacgg cagcaagggg tcgat
//
LOCUS       DATIRT010000028         3056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_101487, whole genome shotgun sequence.
ACCESSION   DATIRT010000028 DATIRT010000000
VERSION     DATIRT010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101487"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VLN25_00610"
     CDS             <1..410
                     /locus_tag="VLN25_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HSQ09108.1"
                     /translation="FAAGLALLSLSGWTAPLSGARRHVLQAALFVVIGAYFVWCWSRS
                     GQTLALKTWQLRVIDDDGRSPSLPRAIGRYLLSWTLFLPGLAYIAIAEPSRAGSLIAL
                     AIGFAALLIPAIVDPARRLLHDRWTGTRIVRDS"
     gene            complement(420..1130)
                     /locus_tag="VLN25_00615"
     CDS             complement(420..1130)
                     /locus_tag="VLN25_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09109.1"
                     /translation="MFGTATPATQKKLSDALGRVIDLRARLHADAALGARWLAVKKWQ
                     SERLGRTYADLLADERYRDSSEFFLEELYGARDFEQRDEEAQRVVPKLARMLPARAIE
                     TLVLAVELDELAEGLDAELARIVQMPITAQRYADAYAQVGSQEQREHQIELVDEIGSA
                     LDRLARIPMLAPMLGMMKGPAEAWGYGHLHRFLYRGFNAFARMGGARKFLATIKHREL
                     AINARLFAGEPDPFRPVD"
     gene            complement(1158..2525)
                     /locus_tag="VLN25_00620"
     CDS             complement(1158..2525)
                     /locus_tag="VLN25_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSQ09110.1"
                     /translation="MLDQRLDQLPADGEHRVERLHRVLELMTEVVIPEPKSRIDSYPH
                     ELSGGQQQRVMIAMAIACEPKLLIADEPTTALDVTVQRQILELIKRLQDRHRMSVLFI
                     SHDLGLVGEIADHVVVMRDGIVREKGRVAEIFEAPQDAYTKALLLCRPRLDVKPKRLP
                     VIDDFMQGKPVSQIVVTEPRIARAVAAAPLIEAKGLRKEYRLKTGLFARKIVEAVKDA
                     SFALYPGRTLGVVGESGSGKTTVGMLLMRLVDATAGQVLYQGKDLLTLTDAEMMAFRR
                     KIQIIFQNPYASLNPRFTVGQILTEPMKIHGIGANDADRFDQATALAQRVGLAADAIY
                     KYPHEFSGGQRQRIAIARCLTMKPDVVICDESVSALDVSVQATVLNLLLDLQDEFGMT
                     YIFISHDLAVVKYMADDMLVMSEGAIVERGSSEAIYADPQHDYTRKLLSAIPRGYAPR
                     AQRAA"
     gene            2454..2987
                     /locus_tag="VLN25_00625"
                     /pseudo
     CDS             2454..2987
                     /locus_tag="VLN25_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT          494 a         1031 c          994 g          537 t
ORIGIN      
        1 cctttgccgc cgggctcgcc cttctctcac tgtccggctg gactgcgccc ctgagcggtg
       61 cacgccggca cgtcctgcaa gcggcactgt tcgtcgtcat cggggcctat tttgtctggt
      121 gctggagccg ttccggccag acgctggcgc tcaagacctg gcaactgcgg gtcatcgatg
      181 acgacggtcg cagcccctcg ctgccacggg ccattgggcg atatctgctg tcgtggaccc
      241 tgttcctgcc gggcctcgcc tacatcgcga tagccgagcc ctcgcgcgca ggcagcctga
      301 tcgctctcgc gatcggcttt gccgcgttgt tgatccccgc catcgtcgat ccggcgcgac
      361 gcctcctgca cgaccgctgg accggtacgc gcatcgtccg cgacagctga gaacccgggt
      421 cagtcgaccg gccggaacgg atccggctcg cctgcgaaca gacgcgcatt gatcgcgagt
      481 tcgcggtgct tgatcgtagc gagaaacttg cgcgcaccgc ccatgcgcgc gaacgcgttg
      541 aatccccggt acaggaagcg gtgcaggtga ccgtatcccc aggcctccgc cggccctttc
      601 atcatgccga gcatcggtgc cagcatcgga atccgcgcca ggcgatcgag cgcgctgcca
      661 atttcatcga ccagttcgat ctggtgttcg cgttgctctt ggctgccgac ctgcgcgtag
      721 gcatccgcgt aacgctgcgc cgtgatcggc atctgcacga tgcgcgccag ttcagcgtcg
      781 aggccctcgg ccagttcatc aagttcaacg gcaagcacca gggtttcgat tgcacgcgcc
      841 ggcagcatgc gggccagctt gggaacgacc cgctgggcct cctcgtcgcg ctgctcgaag
      901 tcgcgtgccc catagagctc ttcgaggaag aactccgaag aatcgcggta gcgttcgtcg
      961 gcgagcaggt cggcgtaggt ccgtccgagt cgttccgact gccacttctt caccgccagc
     1021 caacgggcgc cgagcgcggc atcggcatgc aggcgtgcac gcaggtcgat gacgcgtccc
     1081 agcgcgtcgg agagcttctt ctgtgtagcc ggagttgcgg tgccaaacac ggcgggaatt
     1141 gtcgcgggcg acgtacccta ggcggcgcgc tgcgcgcgcg gcgcgtagcc gcgaggaatg
     1201 gctgacagca gcttgcgagt gtagtcgtgc tgaggatcgg catagatcgc ctcagacgac
     1261 ccgcgctcga cgatcgctcc ctcgctcatg acgagcatgt cgtccgccat gtacttgacg
     1321 accgcgaggt cgtggctgat gaagatgtag gtcatgccga actcgtcctg caggtcgagc
     1381 aacaggttca gcaccgtggc ctgcactgat acgtcgagcg ccgacaccga ctcgtcgcag
     1441 atcaccacat cgggcttcat cgtcaggcac cgtgcgatgg cgatgcgctg ccgctgtcct
     1501 ccggaaaatt cgtgcggata cttgtagatg gcatcggccg ccaggccgac gcgctgcgcc
     1561 agcgcggtgg cctggtcgaa gcgatccgcg tcattcgcgc cgatgccatg gatcttcatc
     1621 ggctcggtca ggatctggcc tacggtaaag cgcggattga ggctcgcgta ggggttctgg
     1681 aaaatgatct ggatcttgcg gcggaacgcc atcatctcgg cgtcggtcag ggtcagcagg
     1741 tccttgccct ggtacagcac ctgccctgcg gtcgcatcca ccagccgcat cagcagcatg
     1801 ccgacggtgg tcttgcccga gccggattct ccgaccacgc cgagagtccg ccctggatac
     1861 agggcaaagc tcgcatcctt caccgcctcg acgatcttgc gtgcgaaaag ccccgtcttg
     1921 agccggtact ccttgcgcag ccccttggct tcgatcagcg gcgccgcggc caccgcccgc
     1981 gcgatgcgcg gctcggtcac gacgatctgc gacaccggct tgccctgcat gaagtcgtcg
     2041 atgaccggca ggcgcttcgg cttgacgtcc aggcgcgggc ggcacagcag cagggccttg
     2101 gtgtaggcat cctgcggcgc ctcgaagatc tcggcgaccc gacctttttc gcgcacgatg
     2161 ccgtcacgca tcaccacgac gtggtcggcg atctcgccca ccagcccgag gtcgtggctg
     2221 atgaacagca cgctcatgcg gtgacgatcc tgcaggcgct tgatcagttc gaggatctgc
     2281 cgctggacgg tcacgtcgag tgcggttgtc ggctcatcgg cgatcaaaag cttcggctcg
     2341 caggcaatcg ccatcgcgat catcacgcgc tgctgctgcc cgcccgaaag ctcatgcgga
     2401 tacgagtcga tgcgcgactt cggctccggg atgacgacct cggtcatcag ctccaggacc
     2461 cgatgcagtc gctcaacccg gtgttcaccg tcggctggca actggtcgag ccgctggtca
     2521 agcacatggg cttgtcgaaa aaggccgcgc tggtgcgggc cgagcagttg ctggtcgagg
     2581 tcggcatgcc cgagcccaag cgccggctgg ccacctaccc gcacgagatg tcgggcggcc
     2641 agcagcagcg ggtgatgatc gcgatggcgc tcgcctgcga gcccaagctg ctgatcgccg
     2701 acgagccgac cacggcgctg gacgtcacca tccagcgcca gatcctcgag ctgctgggca
     2761 agctgaaggc cacgcaccgg atgagcgtgc tcttcatcag ccacgacctc ggcctggtgg
     2821 gcgagatcgc cgaccgcgtg gtggtgatgc gcaacggcat catccgcgag cagggcccgg
     2881 tggcgcgcat cttcgagagc ccgcaggacg actacaccaa ggcgttgctg gcctgccggc
     2941 ccagcctgga gggcaacccg gcgcggctga tggtcatcga cgaccaccgc gccgcgtcgg
     3001 tgcgtgctgc gcagggccag gcggcgctgg ccggcgccgc tgcgcaggcc aaggac
//
LOCUS       DATIRT010000029         3434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_494747, whole genome shotgun sequence.
ACCESSION   DATIRT010000029 DATIRT010000000
VERSION     DATIRT010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494747"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..612)
                     /locus_tag="VLN25_00630"
     CDS             complement(148..612)
                     /locus_tag="VLN25_00630"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HSQ09111.1"
                     /translation="MTLSLDKKVSDFQAMSTAGEIRLSALKGSIVVLYFYPKDNTPGC
                     TTEGVDFAASHAKFKRAGAVVLGVSRDSIKSHEGFKAKMGFPFELISDPDESLCKQFD
                     VMKMKNMYGKQVHGIERSTFVIDGAGVLRREWRGVKVPSHVAEVLAFVQSIA"
     gene            complement(620..994)
                     /locus_tag="VLN25_00635"
     CDS             complement(620..994)
                     /locus_tag="VLN25_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HSQ09112.1"
                     /translation="MKLHADSPTALNTVTAYGSGFIEVNQIRHSCPLLIAPDRPVEPW
                     DVAGFDSLTADDFARLRDRAPELVLLGTGTRQRFAHPRLLRPLTDARIGCESMNSQAA
                     CRTYNILMGEGRKVLAALLLDD"
     gene            1167..2381
                     /gene="alaC"
                     /locus_tag="VLN25_00640"
     CDS             1167..2381
                     /gene="alaC"
                     /locus_tag="VLN25_00640"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HSQ09113.1"
                     /translation="MVEFPRIKRLPPYVFNITGELKMAARRRGEDIIDMSMGNPDGPT
                     PKHIVDKLVEAAQRQDTHGYSVSKGIPRLRRAITNWYQTRYGIEFDPDSEAIVTIGSK
                     EGLAHLMLATLDRGDTVLVPNPSYPIHIYGAIIAGADIRSVRMDGGVDFFAELERAIR
                     ESLPKPKMMILGFPSNPTAQCVDLEFFRRVIALAREHGIYVVHDLAYADITFDGFRAP
                     SIMEVEGAREVAVEFFTMSKSYNMAGWRIGFMVGNKDLVSALARIKSYHDYGTFTPIQ
                     VASIAALEGPQDCVEEIRREYQRRRDVLARGLHEAGWMVDVPKASMYIWAKIPEPYRE
                     MGSLEFAKKVLADAKVAVSPGIGFGDYGDTHVRFALIENEHRIKQAVRGIKDMLRKDG
                     LLVPRVPDKAAA"
     gene            2433..>3434
                     /locus_tag="VLN25_00645"
     CDS             2433..>3434
                     /locus_tag="VLN25_00645"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HSQ09114.1"
                     /translation="MNPIKVGLLGLGTVGRGTLSVLRRNQHEITRRAGRGIEIAAVAV
                     RDAAKARTFLGGDLPISTEPLDVIRRPDIDIVVELIGGYEPARTLVLEAIALGKHVVT
                     ANKALLARHGNEIFEAARARGVMVAFEAAVAGGIPIIKALREGLTANRIEWLAGIING
                     TSNFILSEMRSGGQPFEVVLAEAQRLGYAEADPTFDIEGIDAAHKLTLLAAIAFGVPI
                     NFEAATIEGIARLAATDIKYAEQLGYRIKLLGIAKRTAQGIELRVHPTLVPMQRLIAS
                     VEGAMNAILVKGDAVGTTLYYGPGAGSEPTASAVIADLVDVTRMLTADPENHVPHLAF
                     "
BASE COUNT          644 a         1057 c         1129 g          604 t
ORIGIN      
        1 ggcaggctcg cggtccgctc ggggcgggcc ggttcggagg gaatggcaac gagcagggtg
       61 gcgggcttgg tcggagcttt cggcaggggc atcagagggg cggcagagcc gtgaggggga
      121 atgggggttg cacaggcagc aatgcggtca ggcaatcgac tggacgaagg cgagcacctc
      181 ggcgacatgg ctcggcacct tcacaccccg ccattcgcgc cgcagcacgc cggcgccgtc
      241 gatcacgaag gtgctgcgct cgatgccgtg cacctgcttg ccgtacatat tcttcatctt
      301 catgacgtcg aactgcttgc acagcgattc gtcggggtcg gatatcagtt cgaaggggaa
      361 gcccatcttc gccttgaagc cctcgtgcga cttgatgctg tcgcgcgaca cgcccagcac
      421 gacggcaccg gcgcgcttga actttgcgtg actggcagcg aagtcgacgc cctcggtggt
      481 gcatccgggg gtgttgtcct tgggataaaa gtacaggacc acgatgctgc ccttcagggc
      541 tgacagccgg atctcgccgg cggtggacat ggcctggaag tcggatacct tcttgtcgag
      601 ggagagggtc acggatgcct cagtcgtcga ggagcagggc tgccagcacc ttgcggccct
      661 cgcccatcag gatgttgtag gttcggcagg cggcctgcga gttcatcgac tcgcagccga
      721 ttcgtgcgtc tgtaagcgga cgcagcagcc gcgggtgcgc aaatcgttga cgcgttcccg
      781 tgccgagcag cacaagttcc ggagcgcggt cgcgcagccg ggcgaagtca tctgcagtaa
      841 gggaatcgaa acctgcaaca tcccaaggct cgaccggacg atcgggcgcg atgagtaaag
      901 gacaggaatg gcgaatctgg ttgacttcaa tgaagccaga gccgtaggcg gtaaccgtgt
      961 tgagcgctgt cggtgagtcc gcgtgcagct tcaattcgct ccgggcttcc ttgcgatcga
     1021 ccgcgggctg cggatcgatg cgagaacagt tcgggtacat tataggcaca cgttgcgccg
     1081 cacaagcgcg agccattcgg ctggaaatcg caccggcgtg ttcggtttgc gacgcctttg
     1141 caacactgtg tccgaggtcc ggggagatgg tcgagtttcc gcgcatcaag cggctgccgc
     1201 cgtatgtctt caacatcaca ggtgaactga agatggccgc gcggcgtcgc ggcgaagaca
     1261 tcatcgatat gagcatgggt aatccggatg gacccacacc caagcacatc gtcgacaagc
     1321 tggtcgaagc cgcacagcgg caggataccc atgggtactc ggtgtccaag ggcatacccc
     1381 ggctgcggcg cgccattacg aactggtacc agacgcgtta cggcatcgag ttcgacccgg
     1441 actccgaggc gatcgtcacg atcggttcga aggagggcct ggcacacctg atgctggcga
     1501 cgctggatcg cggcgacacc gtgctggtac cgaacccaag ctacccaatc cacatctacg
     1561 gcgcgatcat cgccggtgcc gacatccgct ccgtgcgcat ggacggcggc gtcgacttct
     1621 tcgccgaact cgagcgcgcg atccgcgagt ccttgcccaa gccgaagatg atgattctcg
     1681 gctttcccag caatccgact gcgcaatgcg tcgacctcga attcttccgc cgcgtgattg
     1741 cgcttgcgcg cgagcacggc atctacgtcg tgcacgacct ggcctatgcg gatatcacct
     1801 tcgatggctt ccgcgctcct tccatcatgg aggtcgaagg cgcacgcgag gtggcggtcg
     1861 agttcttcac gatgagcaag agctacaaca tggccggttg gcgcatcggc ttcatggtcg
     1921 gcaacaagga cctggtgtcg gcgctcgcgc gcatcaagag ctatcacgac tatggcactt
     1981 tcacgccgat ccaggtcgca tcgattgccg cgctcgaggg gccgcaggac tgtgtcgagg
     2041 agatccggcg cgagtaccag cggcgccgcg acgtgctcgc gcgcggactg cacgaggcgg
     2101 gatggatggt cgacgtgccc aaggcctcga tgtacatctg ggcgaagatc ccggagccgt
     2161 accgcgaaat gggatcgctc gaattcgcca agaaggtgct ggccgacgcc aaggtggccg
     2221 tgtcgcctgg aatcggcttc ggcgactatg gtgacaccca tgtgcgcttt gccctgatag
     2281 aaaatgaaca ccgcatcaag caggcggtgc gtggcatcaa ggacatgttg cgcaaggatg
     2341 gcctgctggt tccgcgcgtt cccgacaagg ctgcggcgta agccgatccc tgaaccctcc
     2401 ggcgcctgcg tgcgtcgcct ggcctcacca cgatgaatcc gatcaaggtc ggtctgctcg
     2461 gactgggcac cgttggccgc ggcacgttga gcgtgctccg gcgcaatcag catgagatca
     2521 cccgacgtgc cggtcggggt atcgagatcg cggccgttgc ggttcgcgat gccgccaagg
     2581 cgcgtacgtt tctcggcggc gatctgccaa tttcgacaga acccctcgac gtgatccggc
     2641 gaccggacat cgacatcgtc gtggaactga tcggcggcta cgagccggcg cgcacgctgg
     2701 tgctcgaagc gatcgccctc ggcaaacacg tcgtcacagc gaacaaggca ctgctcgcgc
     2761 gccatgggaa cgagatcttc gaggcggcgc gggcgcgcgg cgtgatggtc gctttcgagg
     2821 cggcggtggc gggcggcatc cccatcatca aagccctgcg cgaaggcctg actgcaaatc
     2881 gcatcgagtg gctcgccggc atcatcaacg gcaccagcaa tttcattctg tccgaaatgc
     2941 gctcgggcgg gcagccgttc gaggtcgtgc tggctgaagc gcagcggctc gggtatgcgg
     3001 aagccgatcc caccttcgac atcgagggga tcgatgcggc ccacaagctg acgctgctgg
     3061 ccgcgattgc cttcggagtg ccgatcaact tcgaagcggc caccatcgag ggaattgcgc
     3121 gacttgccgc gaccgacatc aagtacgccg aacaactcgg ttaccgcatc aagctgctcg
     3181 gcatcgcaaa gcgcactgcg cagggcatcg aattgcgcgt ccatccgacg ctggtaccga
     3241 tgcagcgcct catcgccagc gtcgaagggg cgatgaacgc catcctcgtc aagggcgacg
     3301 cagtgggaac gacgctgtac tacggcccgg gcgcgggttc ggagccgacc gcctcggccg
     3361 tgatcgccga tctcgtcgac gtgacgcgca tgctgaccgc ggacccggaa aaccatgtcc
     3421 cgcacctggc attc
//
LOCUS       DATIRT010000030         2092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_3282, whole genome shotgun sequence.
ACCESSION   DATIRT010000030 DATIRT010000000
VERSION     DATIRT010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3282"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            374..>2092
                     /locus_tag="VLN25_00650"
     CDS             374..>2092
                     /locus_tag="VLN25_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020833093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HSQ09115.1"
                     /translation="MPSSAPGVAASDATAEDGARPFRNFADGLGAQSPMRAAITAATR
                     RSEPECLARLLEAARLPADQAAAAHALASDLARRLRARKVGSGREGLVQGLVQEFSLS
                     SQEGVALMCLAEALLRIPDTVTRDALIRDKIGDDDWESHLGRSRSLFVNAATWGLLLT
                     GKLVSTHTERGLASALARVVARSGEPLIRRGVDMAMRLMGEQFVTGETIAEALSYARR
                     REAEGFTYSYDMLGEAAMTAADSKRYLTSYEESIHAIGRTAAGRGIYDGPGISLKLSA
                     LHPRYSRAQRARVLEELYPRLRALALLARSYDIGISIDAEEADRLELSLDLIERLCTE
                     PELAGWNGVGFVVQAYLKRAPFVLDYLIDLARRSQRRLMIRLVKGAYWDAEIKRAQID
                     GLEDFPVYTRKAYSDVSYLACARKLLAAPEAIYPQFATHNAHTVAAIYHAAGPDYWRH
                     QYEFQCLHGMGEPLYEQVVRTKEGGLARPCRIYAPVGTHETLLAYLVRRLLENGSNSS
                     FVNRIANESIPIDELVADPVMTVEQMARAEGVLGASHPAIALPPSLFGAARRNSRGLD
                     LASEQQL"
BASE COUNT          338 a          690 c          711 g          353 t
ORIGIN      
        1 ccgatcggcg aagccacatc gcactccagc gcgacgaact cgcgatcgaa ccactcgcgc
       61 gcagtgtgga tcgactgctt ctctacgtcc tctgacgcga gcttgaactc gaattcactg
      121 tccttgctga acgacacgat cacgtgatga tggcgcatct gactctcctg gcgtggccca
      181 ggagcccgcc gtagcagaat tctagcggtg cggcgtgcgc ggcgtgccgg aactgtgaac
      241 cgcacatcgg acgagtttcg ggcacgcggc cggtgggcgg gagaggtcgt tgccacaagc
      301 gttgccacaa gcgtcgcaac gcggtcagaa tgctcgtccg caacttccag ggtactgagg
      361 gatgaccccc gccatgccat cgagcgcacc cggcgttgcc gcttccgacg cgaccgccga
      421 ggacggcgcg cgaccgtttc gcaactttgc ggacggcctc ggcgcacagt ctccgatgcg
      481 cgccgcgatc accgccgcga cccgccggtc cgagcccgaa tgccttgccc ggctgctgga
      541 agcggcgcgc ctgcctgccg atcaggccgc cgcagcgcat gcgctggcca gtgacctggc
      601 acggcggctg cgagcacgca aggtggggtc gggtcgcgag ggcctggtgc agggcctggt
      661 gcaggagttc tcgctgtcgt cgcaggaggg cgtggctctg atgtgtctgg ccgaagcgct
      721 gcttcgaatt ccggacacgg tcacgcgcga tgcgctgatt cgcgacaaga tcggcgatga
      781 cgactgggag tcgcacctcg gccgaagccg gtcgttgttc gtcaatgcgg ccacgtgggg
      841 gctgctgctg accggcaagc tggtgtcgac ccacaccgag cgcgggctgg ccagcgcgtt
      901 ggcacgcgtg gtcgcgcgca gcggcgaacc gctgatccgc cgcggtgtcg acatggcgat
      961 gcgtctgatg ggcgagcagt tcgttaccgg cgaaacgatt gcggaagcgc tttcgtacgc
     1021 gcgtcgccgc gaggccgaag gcttcactta ttcgtacgac atgctcggtg aagcggcgat
     1081 gacggccgcg gactcgaagc gctacctcac ctcgtacgag gagtcgatcc atgccatcgg
     1141 gcgcacggcg gccgggcgcg gcatctacga cgggccgggt atctcgctga agctgtcggc
     1201 gctgcacccg cgttacagcc gcgcccagcg tgcgcgtgtg ctggaggaac tgtatccgcg
     1261 cctgcgcgcg cttgcgctgc tggcgcgcag ctacgacatc ggcatcagca tcgatgccga
     1321 ggaggcggac cgtctcgaac tgtcgctgga cctcatcgag cggctttgca ccgagcctga
     1381 gctggccggc tggaacggcg tcggcttcgt cgtgcaggcg tacctgaagc gtgcgccctt
     1441 cgtgctcgat tacctgatcg accttgcgcg tcgtagccag cgccggctga tgatccggct
     1501 ggtcaagggt gcgtactggg acgcggagat caagcgcgcg cagatcgacg ggctggagga
     1561 tttccctgtc tatacgcgca aggcgtactc cgatgtctcg tacctcgcgt gcgctcgcaa
     1621 gcttctcgcg gcgcccgagg ccatctaccc acagtttgcg acccacaacg cacacacggt
     1681 ggcggcgatc taccacgcgg ccggaccgga ctattggcgg caccagtacg agttccagtg
     1741 cctgcacggc atgggcgaac ccctgtacga gcaggtcgtg cggacgaaag agggcggcct
     1801 cgcacgaccc tgccggatct acgcaccggt gggtacgcat gaaaccctgc tcgcctacct
     1861 ggtgcgccgg ctgctggaga acggctctaa cagctccttc gtcaatcgca tcgccaacga
     1921 atcgatcccg atcgacgagc tggtggccga tccggtgatg acggtcgagc agatggcccg
     1981 cgccgagggc gtgctcggag cctcgcatcc ggcgattgcg ttgccgccgt cactgttcgg
     2041 cgcggcgcgg cgcaactcgc gcggactcga ccttgccagc gagcagcaac tt
//
LOCUS       DATIRT010000031         2072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1773167, whole genome shotgun sequence.
ACCESSION   DATIRT010000031 DATIRT010000000
VERSION     DATIRT010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1773167"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VLN25_00655"
     CDS             <1..646
                     /locus_tag="VLN25_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HSQ09116.1"
                     /translation="VVRILGTAQISSPLVDLLAILPLISIVWGNLAAMRQPSFRRMIA
                     YSSIAHAGYLFFAFLGDGSTRLQAVTFYLLAYGLMNALAFASLPAGNDPRRDRLDALK
                     GLYHRQPYAAILLGIAMLSLAGIPPFPGFVAKFLIFKNVIAAGYTLYAVLGLVGSYLG
                     IYFYLRVIQLMFMGAQPEGVPAQGRIAVAAGVLCLVPTIALTLFPGAVIALLGP"
     gene            924..>2072
                     /locus_tag="VLN25_00660"
     CDS             924..>2072
                     /locus_tag="VLN25_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HSQ09117.1"
                     /translation="MNDTKQGETELRSLPADLGAGDPEASAGAIPNLIAGLVETETWR
                     NAATVLMGALLIGLGYWAYTGVRTALADTRAASLQALLGTVVKGLDVWIDEHMTEVQR
                     VARDPDVVARAARLAAERRDPAAAIGRCTQEAEELSARVLLSLSTNDVAVFRIVDRGG
                     LVLASKDPLRCGQRMRLGRFLERLDLAFEGKSQFVRPYPDREVSVASPSGARRPVAWF
                     LAPIRVGNGAPLAVLAMGVETDRELETIFSTARPGETAEAYAFGDDGLMLTSSRFGEQ
                     LRSVGALPATDTGDGAMQVHVRDPGGELVAGFTPKLEPAARPLTQAAALAIAARGKTI
                     AADRSGLIATPYRDYRGTQVIGAWQWLPAYDVGVAAEISESEAFSTLRY"
BASE COUNT          296 a          696 c          706 g          374 t
ORIGIN      
        1 cgtcgtgcgg atcctcggca cggcgcagat cagcagtccg ctggtcgacc tgctcgcgat
       61 cctgccgctg atctcgatcg tgtggggcaa cctggccgcg atgcggcagc cgagcttccg
      121 ccgcatgatc gcgtactcgt cgattgcgca tgccggttac ctgttcttcg cgttcctcgg
      181 cgatggctcg acgcgactgc aggcggtgac cttttacctg ctcgcctacg ggctgatgaa
      241 cgcgctggcg ttcgcgtcgc tgcccgctgg caacgatccg cggcgcgacc ggctcgatgc
      301 gttgaagggc ctgtaccacc gccagcccta tgcagcgatc ctgctcggca tcgcgatgct
      361 gtcactggct ggcattccgc cattccccgg attcgtcgcg aagttcctca tcttcaagaa
      421 cgtgatcgca gcggggtaca cgttgtacgc ggtgctggga ctcgtgggca gctatctggg
      481 tatctacttc tacctgcgcg tgatccagct gatgttcatg ggcgcgcaac cggaaggtgt
      541 cccggcgcag ggccgtatcg ccgtcgctgc gggggtgctt tgccttgtac cgacgatcgc
      601 gctgacgctt ttccctggcg cggtgatcgc tctgctcgga ccctgagtct cagcggtccg
      661 caggaccact gctcgcggcc agtcgcgtgc acgcaatttt ccggcgcctc ggtgccggcg
      721 cccgcaattc gtgtcatcgg tacgggcttg gcgcgttagc atgcacgcct gtgcgttagc
      781 cgggcacgca atcctgatga gtcgagtatc cgcggtggtt cccagcagcg gcggacgctg
      841 gcgcccgtga acgcctgcgc gccgctctgg agttcgagtt cgactcgccc gagccgcggg
      901 ccgtcctgcc ggggatggac cgcatgaacg acacgaagca gggcgagacg gaactgcgtt
      961 cgctgccggc cgacctcgga gccggcgatc ccgaggcctc ggcgggtgca ataccgaacc
     1021 tgatcgccgg cctcgtcgaa accgagacct ggcgcaacgc cgccaccgtg ctgatggggg
     1081 cgctgttgat cggcctcggc tactgggcct acaccggcgt gcgtacggca ctggccgaca
     1141 cccgcgctgc gagcctgcag gcgctgctcg gcaccgtcgt caagggcctc gacgtctgga
     1201 tcgacgaaca catgaccgag gtgcagcggg tggcgcgcga tccggacgtc gtcgctcgcg
     1261 ccgcgcggct tgctgccgaa cgccgcgatc cggcggcagc tatcgggcgc tgcacgcagg
     1321 aggccgagga actgagtgcg cgggtgctgt tgtcgctctc cacaaacgat gtcgccgtgt
     1381 tccgtatcgt cgaccgcggt ggccttgtgc tcgcgtcgaa ggacccgctg cgctgcggcc
     1441 agcggatgcg cctgggtcga ttcctcgaac gactcgatct ggcgttcgag ggtaagtccc
     1501 agttcgtgcg gccgtatccg gatcgcgaag tttcggtcgc gagtccgtcc ggcgctcgac
     1561 gcccggtcgc ctggttcctc gcaccgatcc gcgtgggtaa cggcgccccg ctggcggtgc
     1621 tggccatggg cgtcgagacc gaccgcgaac tcgagaccat cttctcgacg gcgcgacccg
     1681 gcgaaaccgc cgaggcctac gcgttcggtg acgacggcct gatgctgacc tcgagccgct
     1741 ttggagagca gctcaggtcc gtcggtgccc tgccggcgac cgacacgggc gacggagcaa
     1801 tgcaggttca cgtgcgcgac ccgggaggcg aactcgtcgc agggttcacc ccgaagctcg
     1861 agcctgctgc aaggccattg acgcaggcgg cagcgctggc gatcgccgcg cgcgggaaga
     1921 cgattgcggc cgaccgcagc gggctcatcg cgacacccta tcgtgactat cgcggcaccc
     1981 aggtaatcgg cgcctggcaa tggctgcctg cctacgatgt cggggtggcc gccgaaattt
     2041 ccgagagcga ggcgttctcg acgctgcgct at
//
LOCUS       DATIRT010000032         3883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_3501, whole genome shotgun sequence.
ACCESSION   DATIRT010000032 DATIRT010000000
VERSION     DATIRT010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3501"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VLN25_00665"
     CDS             <1..193
                     /locus_tag="VLN25_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006384027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09118.1"
                     /translation="RFGEAALRELGVPTDEAAEIAAEVRKRDAARFELEIAGGDVRAG
                     RPLLRGNKWKPTPLIEPRH"
     gene            complement(471..1664)
                     /gene="metK"
                     /locus_tag="VLN25_00670"
     CDS             complement(471..1664)
                     /gene="metK"
                     /locus_tag="VLN25_00670"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HSQ09119.1"
                     /translation="MSSDFLFTSESVSEGHPDKVADQISDAILDAILAQDPRSRVAAE
                     TLVNTGLVVLAGEITTNSHVDYIEVARETIKRIGYDNTEFGIDYKGCAVLVAYDKQSN
                     DIAQGVDRASDDFLNQGAGDQGLMFGYACDETPDLMPAPIYYAHRLVERQADLRRDGR
                     LPWLRPDAKSQITMRYVDGKPHSIDTVVLSTQHAPEMSLGDKMKPEFTEAVIENIIKP
                     VLPKEWLKDTKYLVNPTGRFVIGGPQGDCGLTGRKIIVDTYGGACPHGGGAFSGKDPS
                     KVDRSAAYAARYVAKNVVAAGIARQCQVQVAYAIGVAKPMNITVYTEGTGVIPDEQIS
                     KLVVEHFDLRPRGIVQMLDLLRPIYEKTAAYGHFGRDEPEFTWERTDRAAALADAVGV
                     KRRAA"
     gene            1871..2746
                     /locus_tag="VLN25_00675"
     CDS             1871..2746
                     /locus_tag="VLN25_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HSQ09120.1"
                     /translation="MLKLLFKSVAALSLATAHRVGAFLGELVFWFSPTYRRRLRENLA
                     QAGYSDPKRVREAAAEAGKQGLETAWIWMRPRSDIIARTEVADMRIVDAALADGRPVM
                     FLAPHLGCFEVTAQFYAAFRPEAKARPITVLYRIPRKAVLREIVATGRAAEGLKLAPA
                     EMKGVRMLMKAMKARELVGILPDQVPSQGEGAWAPFFGRWAYTMTLPARLARQFDAIV
                     LFVYGERLPGGRGYRIHLRRLEEPFTGNLAQDAALMNRGLESLIRECPAQYLWGYNRY
                     KVPAGAEAPPQESPA"
     gene            2743..3618
                     /locus_tag="VLN25_00680"
     CDS             2743..3618
                     /locus_tag="VLN25_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008543471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HSQ09121.1"
                     /translation="MNAWGANLGIFLINLTREWPQSARTRLANALSDLFWWVARPRRR
                     VTLTNLRVCFPEMPEAERARIGQRTFRNLVRAALDHSVLWNADRETIENYIKVEGEEH
                     VHDPANRPLIMIAPHFVGLDAGGVRFNTLARGVSIYSRQKNPVWDAWLLKGRQRFNDP
                     VLLARQGIDMRSVVRTVRDGLPLYYLPDMDLGPVNSIFVPFFGIQAATIPMVSRIARM
                     TGARVVMAVTEMTDDGYVLHFEPPWENFPGETVEEDTARMNRELERWVLRLPTQYFWT
                     HKRFKTRPEGKASIY"
BASE COUNT          623 a         1234 c         1327 g          699 t
ORIGIN      
        1 gcgcttcggc gaggcggcgc tgcgcgaact cggcgtgccg accgacgagg ccgcggagat
       61 cgcggccgag gtgcgcaagc gcgacgcggc gcgttttgaa ctcgagattg caggcggcga
      121 tgtgcgcgcg ggacgaccgc tgctgcgcgg taacaagtgg aagccgacgc cgctgatcga
      181 accgcggcac tagggtcggg cgctgtgcga ggaaacggaa aaggcggcgc aggaagcgga
      241 tgcggcggcc gtggccgagc gcggcgctgc cgcacgctga agctcgcagg ccaagtcttc
      301 ctgctgtcgc gggtcggctt agctcaggcg gcgcggcgct tcacgccgac cgcgtcggcc
      361 agcgccaccg cgaggtcgac ttttgactcc ggacgcgctc gtcgcttgac ggccgcttaa
      421 cttcggctgg cgccgaagca agcctcgacg gaagattgat ccgccaccct tcaggcggcg
      481 cggcgtttca ccccgaccgc gtccgctagc gcggccgcac ggtcagttcg ttcccacgta
      541 aattcgggct cgtcgcggcc gaaatggcca taggcggcgg tcttctcata gatcggacgc
      601 agcaggtcga gcatctgaac gatgccgcgc gggcgcaagt cgaagtgctc gacgacgagc
      661 ttgctgatct gctcgtccgg gatcacgccg gttccctcgg tgtagacggt gatgttcatc
      721 ggcttggcca ctccgatcgc gtaggcgacc tggacctggc actgtcgcgc gatcccggcg
      781 gccacgacgt tcttggcaac gtaacgcgcc gcataggcgg ccgagcgatc gaccttcgac
      841 ggatccttgc cggagaacgc accgccgccg tgcgggcagg cgccgccgta ggtgtcgacg
      901 atgatcttcc gtcccgtcag cccgcagtcg ccctgcggcc cgccgatgac gaagcgaccg
      961 gtgggattga ccaggtactt tgtgtccttc agccactcct tcggcagcac cggcttgatg
     1021 atgttctcga tcactgcctc ggtgaactcg ggcttcatct tgtcgcctag gctcatctcg
     1081 ggtgcgtgct gcgtcgagag cacgacggtg tcgatcgaat gcggcttgcc gtcgacgtag
     1141 cgcatcgtga tctggctctt ggcgtccgga cgcagccacg gcaggcgtcc gtcacggcgc
     1201 aggtcggcct ggcgttccac caggcggtgc gcgtagtaga tcggagccgg catcaggtcg
     1261 ggagtctcgt cgcaggcgta gccgaacatc aggccctggt cgccggcgcc ctggttcagg
     1321 aagtcgtcgg aggcgcggtc cacgccctgt gcgatgtcgt tgctctgctt gtcgtaggcg
     1381 accagcaccg cgcagccctt gtagtcgatg ccgaactcgg tgttgtcgta gccgatgcgc
     1441 ttgatcgtct cgcgcgcgac ctcgatgtag tcgacgtggg agttggtcgt gatttctccg
     1501 gccagcacga cgaggccggt gttgacgagg gtctccgcgg cgacgcgcga gcgtggatcc
     1561 tgcgccaaaa tggcgtcaag gatggcgtcc gagatctggt cggcgacctt gtccgggtgg
     1621 ccttccgaga ccgattcgga cgtgaaaaga aaatcgctgg acatggtccg ctcctgtagc
     1681 aatgtatcgg gtgcgttgcg cactcccgag gagcggcgac gctttagcgg tatttgcctc
     1741 ggctatgctg tcgaggatcg ccccgcaagt tgtcggttaa cacggcgaac ggcgattata
     1801 ggtcaagcca cgtactcgcc cgcgcccgca ttgttcccga ctgcccggtg atctccgtcg
     1861 tcgccctgct gtgctgaaac tgctgttcaa gtccgttgcc gcgctgtcgc tcgcgacggc
     1921 gcacagggtc ggtgctttcc ttggggaact ggtgttctgg ttctcgccga cttaccggcg
     1981 gcggttgcgc gaaaacctgg cacaggcggg ctacagcgat cctaagcggg tccgggaggc
     2041 cgccgccgag gccggcaagc agggcctgga aacggcctgg atctggatgc gtccgcgttc
     2101 cgacatcatc gcgcggaccg aggtcgcgga catgcggatc gtcgacgccg cgctggccga
     2161 cggccggccg gtaatgttcc tggcacccca tctgggatgt ttcgaggtca cggcacagtt
     2221 ttatgccgcc ttccgcccgg aggcgaaggc gcggccgata acggtgctgt atcgcattcc
     2281 gcgcaaagca gtattgcgcg agatcgtcgc gaccggccga gcggccgagg gccttaagct
     2341 cgcccccgcc gaaatgaagg gcgtgcgcat gctgatgaag gcgatgaagg cccgcgaatt
     2401 ggtcggcatc ctgcccgacc aggtcccgtc gcaaggtgag ggcgcgtggg cacccttttt
     2461 cggtcgctgg gcctacacga tgacgttgcc ggcacgcctg gcgcgccagt tcgatgccat
     2521 tgtgttgttc gtctacggtg agcgattgcc gggtggccgc ggttaccgca tccacctcag
     2581 gcgactcgag gagccgttca ccggaaatct cgcccaggat gccgcgctga tgaaccgcgg
     2641 gctggagtcg ttgatccgcg agtgcccggc acagtatctg tggggctaca accgctacaa
     2701 ggtcccggcc ggtgccgagg cgccgccgca ggaatccccg gcatgaacgc ttggggcgcc
     2761 aacctgggca tcttcctgat caacctgacg cgcgagtggc cgcagagcgc gcgcacacgc
     2821 ctggccaatg cgctgtcgga cctgttctgg tgggtggccc gcccgcgccg tcgggtgaca
     2881 ctgaccaacc tgcgagtgtg ctttcccgag atgcccgagg ccgagcgcgc gcggatcggg
     2941 cagcggactt ttcgcaacct tgtacgagcg gccctggatc actccgtgct ttggaatgct
     3001 gaccgggaaa cgatcgagaa ctacatcaag gtcgagggcg aggagcatgt gcacgatccc
     3061 gccaatcgac cgctgatcat gatcgcgccg cacttcgtcg ggctcgacgc ggggggcgtg
     3121 cgattcaaca cgctcgcgcg cggcgtctcg atctactcac ggcagaagaa tccggtgtgg
     3181 gatgcgtggc tgctcaaggg ccggcagcgt ttcaacgatc ccgttttgct ggcgcggcaa
     3241 ggcatcgaca tgcgcagcgt cgtgcgcacg gtcagggacg gcctgccgct gtactacctg
     3301 ccggacatgg acctcggccc ggtgaactcg atcttcgtgc cgttcttcgg tatccaggcc
     3361 gcgacgatac cgatggtctc gcgcattgca cgcatgaccg gcgcgagagt cgtgatggcg
     3421 gtcaccgaaa tgaccgacga cggctacgtg ctgcactttg aaccgccctg ggagaacttc
     3481 cccggcgaga cggtcgagga agacaccgcc cgcatgaacc gggaactcga acgctgggtg
     3541 ctgcggctgc cgacccagta cttctggacc cacaagcggt tcaagacgcg gcccgagggg
     3601 aaggcgtcga tctactagga gcgtctcgaa tgtacgcaca tcccggcgaa gccgggatgt
     3661 gcacgtgcat tcgctccgga gtggttagtc ggctgctccg gagcggccgc gcgctacgcc
     3721 gaaatacaac cccacgcact ccaccccgcg acgcacctcg cagtgccagg attgcctttc
     3781 cctcctcgcc tggcgaggag ggtgcaggga ggagtgcgcc actccctctc gctcggcgag
     3841 agggtcaggg tgagtgctgt gtagttgtat ttcggcggag cgc
//
LOCUS       DATIRT010000033         3637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_495341, whole genome shotgun sequence.
ACCESSION   DATIRT010000033 DATIRT010000000
VERSION     DATIRT010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495341"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..698)
                     /locus_tag="VLN25_00685"
     CDS             complement(207..698)
                     /locus_tag="VLN25_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09122.1"
                     /translation="MSITPRRGCRAMRLAGLALLAALTPLSSAMAQSEGGLYIAGDAG
                     FKFQQAAERGLAQNPGGRRFFVLSVPPQTSAMKKSAPAPLAQLRDRVIAGNGVLLVCQ
                     RDIDKGRIDPAELVPGVVAVRGWPEPGSNEISPGTRYFADEDTSRLPAANEALRRLRS
                     TCS"
     gene            complement(782..1483)
                     /locus_tag="VLN25_00690"
     CDS             complement(782..1483)
                     /locus_tag="VLN25_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09123.1"
                     /translation="MHDVLSTVTVLRAATLSVALAALSASAQTPTTMFSCVANGKTYS
                     ADRPPPECANSEMRELNRDGSVRRVIPRPLTVEEQRARALEAKKKLEEEEKLLAQRRR
                     DRALLEAYATDAEIEAARKKSLEGAEQAIKRSQERADSLAKYRKRLDDEAEFYKKREQ
                     PDSLKRAYALNAQAVTAEERIMAEARGEVARINERFDAERKRYKELLAQGARPVQRNP
                     EADILLDPRFKDATR"
     gene            complement(1476..2153)
                     /gene="pyrE"
                     /locus_tag="VLN25_00695"
     CDS             complement(1476..2153)
                     /gene="pyrE"
                     /locus_tag="VLN25_00695"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008540358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HSQ09124.1"
                     /translation="MVLLRQQFLEFALQAGVLRFGEFKTKAGRLSPYFFNAGLFNRGA
                     LLARLARFYAQTLLAACAEGRVHFDMLFGPAYKGITLAAGTAMALADLGTDVPFAYNR
                     KEAKDHGEGGVIVGAPLAGRVVIVDDVITAGTSVRESVDLIRGAGATPAAVLIALDRM
                     ERAGPDEALSPRSAVQEVEAAYQVPVLAIATLDDVMQFLAAESAQAQQVRAYRPAVER
                     YRERYGA"
     gene            2271..3044
                     /locus_tag="VLN25_00700"
     CDS             2271..3044
                     /locus_tag="VLN25_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008647203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HSQ09125.1"
                     /translation="MLRIVTLNVNGIRSAASKGLFRWLRRIEPDIVCLQEVRADEVDI
                     PEKLRFPDGLHGFFHCAQRRGYSGAAMYTAREPRSVRLGFGSAEFDTEGRYVEAEFKD
                     FTAISVYFPSGSSGPERQDAKYRFLAQFAPHLEKLRASGREIVFCGDVNIAHREIDLK
                     NWRSNRKNSGFLPEEREWLTGVFDGGWTDIFRRLDARPEQYTWWSNRGQAWAKNVGWR
                     IDYQIATPGFAARARRTEIYTAKRFSDHAPLVIDYTGRL"
     gene            3054..3284
                     /locus_tag="VLN25_00705"
     CDS             3054..3284
                     /locus_tag="VLN25_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09126.1"
                     /translation="MRKADFIRSRLPENQRAALGPYVDLNSQPAKMNDIDLRLNRVKV
                     PKGYLSQLNTLREHADDVRKLLHTRVASPPTA"
     gene            complement(3281..>3637)
                     /locus_tag="VLN25_00710"
     CDS             complement(3281..>3637)
                     /locus_tag="VLN25_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09127.1"
                     /translation="CRSSSKNRTPVPPAQRLPMSSRAREPGDAVRRVLENAGILIFAS
                     LLVMSIAMGRYTGALVWCGALMVFFAHAWEGRASRRSETDVASLATEGARPRHYDALR
                     WVGVTLSLLGAAALAV"
BASE COUNT          635 a         1220 c         1156 g          626 t
ORIGIN      
        1 caatgccagc ggcaagccgg tgctattgcg gctcgattac gagtcaggcc atgggcaggg
       61 aagcacgcgt gcgcagttgc aggagcgctc cgccgatctg tatagcttca tgctgtggca
      121 attcggcgtg ccggagttcc agccgtccac caagccctga gtccgtgggc ggcggcgttg
      181 gctcgtcggg cgcgtcgcga ctcgaatcag gaacaggtcg accgcaggcg tcgcagcgcc
      241 tcgttggccg ccggcagccg gctcgtgtct tcgtcggcaa agtagcgcgt gcccggagaa
      301 atttcattgc tgcccggctc gggccagccg cgcaccgcga caacgcccgg cacgagttcg
      361 gcgggatcaa tgcgaccctt gtcgatgtcg cgctggcaga ccagcaacac accgttgccg
      421 gcaatgactc ggtcgcggag ttgcgcgagg ggagctgggg ccgacttctt catcgcactg
      481 gtctgcggtg gcaccgaaag cacgaaaaag cgccgccctc cgggattctg ggccaggccc
      541 cgctcggccg cctgctggaa cttgaagccg gcatcgcccg cgatataaag gccgccttcg
      601 ctctgcgcca ttgctgacga caacggcgtg agtgctgcca gcagggccag cccggccagc
      661 cgcatggcgc ggcacccgcg tcgaggtgtg atggacatgc tcgatctcct gcaaaccgga
      721 cgatcaggcg gaggattccc tgcgcgccag tctgaatccg cccaaggatg cccccgcgcc
      781 attaccgcgt ggcgtccttg aacctcggat ccagcaggat atctgcctcg gggttgcgtt
      841 gcacggggcg tgcaccctgt gccagcagtt ccttgtagcg ttttctttcc gcatcgaagc
      901 gttcattgat gcgcgcgact tccccgcgcg cctcggccat gatccgctct tcggccgtga
      961 cggcctgcgc gttcagggcg taggcgcgct tcaggctatc gggctgctcg cgcttcttgt
     1021 agaactcggc ttcgtcgtcg agtcgcttgc ggtacttagc gagactatcc gcgcgctcct
     1081 gcgagcgctt gatggcctgc tccgcgccct cgagcgactt cttgcgcgcc gcttcgatct
     1141 cggcatcggt ggcgtaggcc tcgagcagtg cccgatcgcg ccggcgctgt gccagtaact
     1201 tctcttcttc ctcgagcttc ttcttggctt cgagcgcgcg tgcacgctgt tcctcgaccg
     1261 tcaggggtcg tggaatcacg cgccgcaccg aaccgtcgcg attcagttcg cgcatctcgc
     1321 tgttggcgca ctccggcggc gggcggtcgg ccgaatacgt cttgccgttg gccacgcagc
     1381 tgaacatcgt ggtgggtgtc tgcgccgacg cggataaagc ggcgagcgca accgagagcg
     1441 tcgcggcgcg gaggaccgtc acggtcgaaa ggacgtcatg caccgtagcg ctcccgatag
     1501 cgttccaccg ccggccgata ggcgcgcacc tgctgtgcct gcgccgactc cgccgccagg
     1561 aactgcatca catcgtccag cgtggcgatg gccaggaccg ggacctggta cgccgcctcg
     1621 acctcctgca ctgcagatcg cggcgacagg gcctcgtcgg ggcccgcgcg ttccatccgg
     1681 tcgagcgcga tcagcacggc ggccggcgtc gctcccgcgc cgcggatcag atcaaccgat
     1741 tcgcgcacgg aggtgccagc cgtaatgaca tcatcgacga tgacgacacg cccagcgagt
     1801 ggcgctccga cgatcacgcc gccctctcca tgatccttgg cttccttgcg gttgtaagcg
     1861 aaggggacgt cggtgcccag gtccgccagc gccatggccg tgccggccgc cagcgtgatg
     1921 cctttgtagg ccggtccgaa cagcatgtcg aaatgcactc gtccctccgc gcacgccgcc
     1981 aacagcgtct gtgcgtagaa acgcgcgagc cgcgccagca gtgcaccgcg attgaagagc
     2041 cccgcattga aaaagtaggg cgacagcctt cccgccttgg tcttgaactc accaaagcgc
     2101 aggacacctg cctgcaatgc aaattcgagg aattgctggc gcagcaacac gatggtcttg
     2161 aggggccccg ccgcccgaag gaatctatcc atctcaattg tgccgcagcg ccaccgcgaa
     2221 aagcatggaa aatgccgtcg aaatcagcac tgcaaaagcg attggaccct gtgctgcgca
     2281 tcgtcacact gaatgtcaac ggcatccgct cggcggcttc caagggactg tttcgctggc
     2341 tgcgcaggat cgagcccgac atcgtctgcc tgcaggaggt cagggccgac gaggtcgata
     2401 tcccggaaaa gctccgattc ccggacggcc tgcacggttt ttttcattgc gcgcagcgcc
     2461 gcgggtactc gggcgccgcg atgtacaccg cccgggaacc tcgctccgtg cgactcggct
     2521 tcgggtcggc cgaattcgac accgagggcc gttacgtcga ggccgagttc aaggacttca
     2581 cggcgatctc ggtgtacttc ccgtcaggct cgtccggacc ggagcggcag gacgccaagt
     2641 accgctttct cgcccagttc gcaccgcatc ttgagaagtt gcgcgccagc ggtcgcgaga
     2701 tcgtgttctg cggcgacgtg aacatcgcac atcgggagat cgacctgaag aactggcgca
     2761 gcaatcgcaa aaactcgggc ttcctgccgg aggaacggga atggctgacc ggcgtctttg
     2821 atggcggctg gaccgatatc ttcaggcgcc tcgacgcgcg tccggagcaa tacacctggt
     2881 ggagcaaccg cggccaggcc tgggccaaga acgtcggctg gcgcatcgat tatcagatcg
     2941 cgacgcccgg cttcgcggcg cgcgcacggc ggaccgagat ctatacggcg aagcgatttt
     3001 ccgatcacgc tccgctcgtc atcgattaca cgggacgcct gtgagcgacc gcgatgcgga
     3061 aggctgactt catccgcagc cgactgccgg aaaaccagcg ggcggcactc ggcccgtacg
     3121 tcgatctgaa ttcacagccg gcgaagatga atgacatcga cctgcgattg aaccgggtca
     3181 aggtcccgaa aggctacctg tcccagttga acacgttgcg cgaacacgcc gacgatgtgc
     3241 gcaagctgct gcacacgcgc gttgccagcc cgccgaccgc ctagaccgcc agcgcggcag
     3301 ctcccaggag ggacagcgtt accccgaccc agcgcagcgc gtcgtagtgg cgcgggcgcg
     3361 cgccttcagt agccagcgac gcgacatcgg tctcgctgcg gcgtgaagca cgtccctccc
     3421 acgcgtgggc aaagaacacc atcagggctc cacaccagac gagcgctccg gtgtagcgac
     3481 ccatcgcgat cgacatcacc agcagagatg caaagatcag gatgccggca ttctcgagga
     3541 cgcgacgcac ggcgtcgccc ggctcgcgcg cgcggctgct catcggcaac ctctgtgcag
     3601 gcggcaccgg tgttcgattc ttcgaactcg accggca
//
LOCUS       DATIRT010000034         2055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_200507, whole genome shotgun sequence.
ACCESSION   DATIRT010000034 DATIRT010000000
VERSION     DATIRT010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200507"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VLN25_00715"
     CDS             <1..849
                     /locus_tag="VLN25_00715"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HSQ09128.1"
                     /translation="LVLGSGGVRSIAAVGIAEVLAAEGLRPDLIVGCSSGALFGATIA
                     MGMTSREALQVATSLWSAELTQQHRWPAYAQLVAPKLFGFDAGFALRDDRRIAQRIRR
                     AFGDWRLEDLPTPLRVVATDARSGERVVLRHGSLTQAVRASVAVPLMFPSVEVDGRRL
                     VDGVVSDPLPIAVASDAAVVVALGFKGVMPRRIDRASRLVAQSSTALINNLQQARIDA
                     ARNAGQQVVEIELALDRHVGLWETRAMPYLFEAGCRAARVQLAQILALARQSSPCLPG
                     YEALAA"
     gene            849..1769
                     /locus_tag="VLN25_00720"
     CDS             849..1769
                     /locus_tag="VLN25_00720"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HSQ09129.1"
                     /translation="MNHELQPSPVRRTLFDGELLQVGYVAVRPVSSACGEIEVSDSNV
                     LALTLSGMFARHDGPRRHVIATPNHALFISAGTPYRLSFPGCIGDRCLVLRFSGEALA
                     QALPVALARDGFDPAVFASHALLEPRTMLARSLLWSRLERGEIDVLEVEERGICLLAA
                     ALHAARMPGRGRTPLRGGAPRRRRQVEAVKEAIAIDPERKWTLGALAGLACVSPGHLA
                     HVFRAEEGSSLYQYVLRSRLARALDAVLDSDTDLTAIALDAGFASHSHFTARFRALFG
                     RTPVELRRGNGATRVKELRRIVTAREGATA"
     gene            1834..>2055
                     /locus_tag="VLN25_00725"
     CDS             1834..>2055
                     /locus_tag="VLN25_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09130.1"
                     /translation="MLSGLVASAGPASPVPHLELAEIQLRAINHRFVNVDIDPDGHLM
                     DALTDEDFLLTDGDGTWHDRAGFLAQVRQR"
BASE COUNT          297 a          668 c          706 g          384 t
ORIGIN      
        1 ctggtgctgg gtagcggtgg ggtgcgcagc atcgccgcgg tcggcatcgc cgaagtactt
       61 gctgccgagg gcctgcggcc cgatctgatt gttggttgca gttcgggagc gctgttcggc
      121 gcgacgattg cgatgggcat gacaagccgc gaggcactgc aagtcgcgac gtccctgtgg
      181 tcggccgagc tgacacagca gcaccgatgg cccgcctacg cacaactggt cgcgcccaag
      241 ctgtttggtt tcgatgccgg cttcgcattg cgtgacgacc ggcgcattgc gcagcgcatc
      301 cggcgcgctt ttggcgactg gcgtctggag gacttgccca caccgctgag ggtggtcgcc
      361 accgacgcca ggagcggcga gcgtgtggtg ctgcgccacg gttcattgac gcaggcggtg
      421 cgcgccagcg tcgctgtgcc gctgatgttt ccgagcgtcg aggtcgacgg gcgcaggctg
      481 gttgacggcg tagtgtccga tccgctgccc atcgccgtcg cttccgatgc cgcagtggtg
      541 gtcgcgctgg gcttcaaggg ggtcatgccg cggcgcatcg accgcgcgtc gcgcctggtg
      601 gcgcagtcga gcacggcact catcaataac ctgcaacaag cccgcatcga cgctgcccgc
      661 aacgctggac agcaggtcgt ggagattgaa ctggcgctgg atcggcacgt gggcctgtgg
      721 gagactcgcg cgatgccgta tctgttcgag gcgggatgcc gtgccgcacg ggtgcaactg
      781 gcccaaatct tggcgcttgc acgtcagtcg tcgccatgct tgcctggcta cgaggcgctg
      841 gcagcgtgat gaaccacgag ctccagccat cgccggtgcg gcgcaccctg ttcgacggtg
      901 agctgctgca ggtcggctac gtggccgtac ggccggtgtc gagcgcatgc ggcgagatcg
      961 aggtgtccga ttccaacgtg ctggcgttga cgctgtcggg catgtttgcc aggcacgatg
     1021 ggccgcgccg gcacgttatc gccacaccga accacgcgtt gttcatctcg gctggcacgc
     1081 cctaccgact cagcttcccg ggatgcatcg gcgaccggtg cctggttctg cggttctccg
     1141 gcgaggcgct cgctcaagcc ctgccggtcg ctcttgcacg tgacggcttc gatcctgcgg
     1201 tcttcgccag ccacgcgctg ctcgagccgc gaacgatgct ggcgcgcagc ctgctctgga
     1261 gtcgtctcga gcgcggcgag atcgacgtgc tggaggtgga agaacgcggc atctgcctgc
     1321 tggcggccgc gctgcacgcc gcgcgaatgc ctgggcgcgg ccgaacgcca ttgcgcggcg
     1381 gcgcgccgcg tcgccggcgc caggtggaag ccgtcaagga agcgatcgct atcgatcccg
     1441 agcgcaagtg gacgctgggt gctctggccg gtctcgcctg cgtctcgccc ggccatctgg
     1501 cgcatgtgtt tcgtgcggaa gaaggcagct ccctgtacca gtacgttctg cgctcgcggc
     1561 tggcccgtgc actggatgcc gtgctcgatt ccgacaccga cctcaccgcg atcgcgctcg
     1621 atgccggttt cgcgagtcac agccacttca cggcccggtt tcgcgcactt ttcgggcgta
     1681 cgccagtgga gttgcggcgc ggcaatggcg caaccagggt caaggaattg cgccggatcg
     1741 tgacagcgcg ggagggggca acggcctagg ctggtctctt cacagcccga ggagacgcac
     1801 ttgcttccga catcgcccat tccagcgctc ttcgtgctgt ccgggctcgt ggccagcgca
     1861 ggacctgcct cgccggttcc acacctggaa ctcgccgaga ttcaacttcg tgccattaac
     1921 catcgctttg tcaacgtcga catcgacccc gacggccatc tgatggatgc gttgactgat
     1981 gaagactttc tgctcaccga cggcgacggc acctggcacg atcgcgcggg ttttcttgcg
     2041 caggtgcgcc aacgg
//
LOCUS       DATIRT010000035         1742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_4146, whole genome shotgun sequence.
ACCESSION   DATIRT010000035 DATIRT010000000
VERSION     DATIRT010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4146"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VLN25_00730"
     CDS             <1..282
                     /locus_tag="VLN25_00730"
                     /EC_number="1.8.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF014907.4"
                     /note="catalyzes the reduction of adenosine
                     5'-phosphosulfate to AMP and sulfite; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylylsulfate reductase subunit alpha"
                     /protein_id="HSQ09131.1"
                     /translation="ELLTMLKEDMNSMGAEDLHQLQRTWELNHRILASESVTHHTLFR
                     KETRWPGYYYRGDHVKLDDENWHCFTLSRYDRQSGKWAMEKAPVHHIID"
     gene            347..793
                     /locus_tag="VLN25_00735"
     CDS             347..793
                     /locus_tag="VLN25_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HSQ09132.1"
                     /translation="MTEIRIDPPAHAAWTLVFASWLVAAVATLGALFFGEVMKVPTCS
                     LCWYQRIFMFPLVFILPFGLFPFDAKVARVALPLAAAGWLIASWHVLLVAGVIPETAT
                     PCTQGVPCSETYFRWFGFVTIPLLAWLAFSFIIALLAAAHFKASKR"
     gene            790..1443
                     /locus_tag="VLN25_00740"
     CDS             790..1443
                     /locus_tag="VLN25_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HSQ09133.1"
                     /translation="MKQKTLFIASAVVLLAAFVVGALVYNIQRADRTAEVVKRNPQAL
                     TRLHSPTLGNADAKVHIVEFLDPACATCAQFSPFVKDLMAANPGRIRLTVRYAPLHQG
                     SEQVVKLLEAARKQGKYWQALEALFATQRQWVQNHVAHAQLAWNSLAGLGLDLDRVRA
                     DMNAPEVTRIVEQDIADTRTLNVTQTPEFFVNGRAMPSFGAEQLKDLVDEEIAKAYR"
     gene            complement(1456..1716)
                     /locus_tag="VLN25_00745"
     CDS             complement(1456..1716)
                     /locus_tag="VLN25_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09134.1"
                     /translation="MEILFLRCPALHGFSVQQGSSVSRERIAQDLQVGLLLADVVWHQ
                     PLNGDQAATLVEEISQELLELVDERPEATALLRGRTFARVLH"
BASE COUNT          302 a          598 c          536 g          306 t
ORIGIN      
        1 gagttgctga ccatgctgaa ggaagacatg aacagcatgg gcgcggaaga ccttcaccag
       61 ctgcagcgca cgtgggagct gaaccaccgg atcctggcct cggagtccgt gacccatcac
      121 acgctattcc gcaaggagac gcggtggccg ggttactact atcggggcga tcacgtgaag
      181 ctcgatgacg agaactggca ttgcttcacc ctctcccgct acgaccggca atccggcaag
      241 tgggcaatgg agaaggcgcc cgtccaccac atcatcgact gacgtcgtct cgcgggcgcc
      301 agctcgccga ctcgaacggg cgcttccgat atgggatgat cggtcggtga ccgagatccg
      361 catcgacccg ccggcgcacg cggcctggac cttggtattc gcgtcctggc tggtggccgc
      421 cgtcgccacc ctgggcgcgc tctttttcgg cgaagtcatg aaagtgccga cctgctccct
      481 gtgctggtat cagcgcatct tcatgttccc gctggtcttc atccttccgt tcgggctgtt
      541 tcccttcgac gcgaaagtcg cgcgcgttgc gctgccgctc gccgcggcag gatggctgat
      601 cgcgtcctgg cacgtgctgc tcgtcgcggg cgttatcccg gagaccgcga cgccctgcac
      661 ccagggcgtg ccgtgctccg agacttattt ccgctggttc ggtttcgtca ccattccgct
      721 gctcgcctgg ctcgcctttt cgttcatcat cgcgctcctc gccgccgcgc atttcaaggc
      781 ctccaagaga tgaaacagaa aacattgttc atcgcgtccg ccgtcgtgct gctggccgcg
      841 ttcgtcgtcg gcgcattggt ctacaacatc cagcgggccg accgcacggc cgaggtcgtc
      901 aagcgcaacc cgcaggcgct gacgcgattg cattcgccga ccctcggcaa cgccgatgcc
      961 aaggtccata tcgtcgagtt cctcgacccc gcctgcgcga cctgcgcgca gttctccccg
     1021 ttcgtgaagg acctgatggc cgccaatccc ggcaggatcc ggctcacggt ccgctatgcg
     1081 ccgctccacc agggctccga acaggtcgtg aaactgctcg aagcggcgag gaaacaaggc
     1141 aagtactggc aggcgctcga ggcgctgttc gccacccagc ggcagtgggt gcagaaccat
     1201 gtcgcgcatg cgcagctcgc gtggaactcc ctcgcagggc tgggcctcga cctcgaccgg
     1261 gtccgagccg acatgaacgc gccggaggtg actcgcatcg tcgagcagga tatcgcggac
     1321 accaggacgc tcaacgtcac ccagaccccg gaattcttcg tcaacggcag ggccatgccg
     1381 agcttcggcg ccgagcagtt gaaggacctc gtcgacgagg aaatcgcgaa ggcgtaccgg
     1441 tagccgctcg cccggtcaat gcaggacccg ggcaaaggtg cggccgcgca gcagcgccgt
     1501 tgcttccggc cgctcgtcca ccagctcgag cagctcctgc gagatctcct cgacgagcgt
     1561 cgcagcctgg tcgccgttca acggctggtg ccagacgaca tcggcaagca acaggccgac
     1621 ttgcagatcc tgcgcgatcc gctcgcggct cacgctcgag ccttgctgca cggagaagcc
     1681 gtggagcgcc gggcaacgca ggaacagaat ctccatgcgt gccgcgagat cggactccag
     1741 cc
//
LOCUS       DATIRT010000036         5547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1085794, whole genome shotgun sequence.
ACCESSION   DATIRT010000036 DATIRT010000000
VERSION     DATIRT010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5547
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085794"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VLN25_00750"
     CDS             <1..535
                     /locus_tag="VLN25_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur subunit"
                     /protein_id="HSQ09135.1"
                     /translation="CAMVINGRPGLACRTLAKDVGPEITLAPLPVFELIGDLSVNTGK
                     WMRAMSERLESWVHMKQKEVDLTRIEERMDPELAEKIYELDRCIECGCCVSACGTARM
                     REDFVGAVGINKITRFRLDPRDARTDADYYDVIGDDSGVFGCMSLLGCHDVCPKDLPL
                     QTQIAFVRKRMVAEGMK"
     gene            573..1973
                     /locus_tag="VLN25_00755"
     CDS             573..1973
                     /locus_tag="VLN25_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HSQ09136.1"
                     /translation="MPRIEKDFLGEKQLPDTAYYGVQTLRGKENFNITGVPMATEPYF
                     VKSFGYVKKAAALANRDLGVLDAKVANAIAAACDRLIAGEMRDQFVTDFIQGGAGTST
                     NMNANEVIANIALESLGHRKGEYQYVNPNDHVNFGQSTNDIYPTAFRLALILRLGSYM
                     EALTKLQEAFFAKGKEFDKVLKMGRTHLQDAVPMSLGQEFHGWGTTMGEEVQRIAEVR
                     QFLHEINLGATAIGTTVTAAPGYPELATKYLSELTGFKFILAGDLIEATSDTGAYVLL
                     SGVLKRTSSKLTKICNDLRMLASGPRCGFNEINLPQMQPGSSIMPGKVNPVIPEVVNQ
                     TSFLVIGLDLTVTLAASAGQLQLNVMEPVITFALFTSIHTMENAVNSLRENCVVGITA
                     NAERTRDMVLNSLGIVTVLKPSLGYKQCADIAKEGYETGKPLHDIVVKERKLLTQEKW
                     DEVFSFENLISPKFEQ"
     gene            2014..3339
                     /locus_tag="VLN25_00760"
     CDS             2014..3339
                     /locus_tag="VLN25_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HSQ09137.1"
                     /translation="MTISIVLQFVVVLAAIWMGSRFSGVGLGLWGAVGLLVLVVAFGV
                     SPTSPPIDVMLIILAVIMAASVMDAAGGIDFLVRIAERIIRANPKYVTIVAPLTTWSF
                     TFVAGTGHIVYPLLPVIYETAHQSGVRPERPMAVATIASQQAITASPVAAATAAMIGL
                     FSEKGLTEWGLPEILMVCVPATLTGVIAAAIVSMFIGKDLKDDPEYQARLKAGKIPVP
                     MAAADRPPLKASAKLSAFLFLAGVALVVLFGFFPELRKLPGAKAPLGMPIVIEIVMMS
                     VAAIMLLFTKVDVDEIPKTATLRAGVVAVIGIFGLAWLGDSFIAAHKDVIVPAIGDWA
                     KVAPWTFAFGLFFASVLLYSQAATTRALMPLGMALGIPPQFLIAMFPSVNGYFFIPTY
                     GSLIAAINFDLSGTTKIGKYVLNHSFMIPGIVATATAVITGLFLAKLMF"
     gene            3416..4126
                     /gene="pepE"
                     /locus_tag="VLN25_00765"
     CDS             3416..4126
                     /gene="pepE"
                     /locus_tag="VLN25_00765"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HSQ09138.1"
                     /translation="MRLLLLSNSKTEGKGYLEHAAGWLRDFLPTGVDEVLFIPYAGVT
                     KTHYDYVDQVAPVFASLGASVSGLHRAHDLVAAIRNADAIVVGGGNTWNLLRELRAHR
                     LVLPAIRQCVLAGVPYIGWSAGSNIACPTIMTTNDMPICDPGGFDALALVPFQINPHY
                     QHGNPPGFKGETREERIREFGVLHPDSWVAGLREGTGFLVEDRSMRLLGDKECRIFRG
                     AEEPRERTSADDFSFLLE"
     gene            4130..>5547
                     /locus_tag="VLN25_00770"
     CDS             4130..>5547
                     /locus_tag="VLN25_00770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HSQ09139.1"
                     /translation="MAGTVVVAGSASRTRDLQRRIRRVGLVAGPLVALMFYWLLPHEY
                     RATDGTDVVFGHTGRATLAMMGWMATWWMTEAVEIEVTSLLPIALFPILGVMPLGKTT
                     ANYGADVIYLFLGGFVIALAIARCGLDRRIAFFTLRLVGTRPKAIVAGTMGATAFLSM
                     WVSNTATAAMMIPIVLSVIDVALRRRTGKGLAEHGGIPDDDRDMRNFALSALLGVAYA
                     ASIGGLGTIIGSPPNGILVRFIEQTSGIQVSFVKWMLIGVPVMLAFLPLAWFLNTSVL
                     FPTRMKEIEGGRDYVRDEWAKLGPLSRGERATLAVFGMAVFLWVTAPALKQWELGGIA
                     PLAGLSDAGIAIIAAITLFLITVDRERGESLMDWTTAAKLPWGVLILFGGGLALESAT
                     EANGVPGYIGSLAAGFSGWPVWAVLLVIIAIMVFMSELTSNTAQVATMLPILAALAPV
                     LGVPTALMLVPATIAASCAFMMP"
BASE COUNT          991 a         1721 c         1783 g         1052 t
ORIGIN      
        1 ctgcgcgatg gtcatcaacg gccggccggg gctggcctgc cgcacgctgg ccaaggacgt
       61 cggaccggaa atcacgctgg cgccattgcc ggtgttcgag ctgatcggcg acctgtcggt
      121 caacaccggc aagtggatgc gcgcgatgag cgagcgactc gagtcctggg tccacatgaa
      181 gcaaaaggaa gtggatctca cccgcatcga ggagcggatg gatccggagc tggcagagaa
      241 gatctacgag ctcgatcgct gcatcgagtg cggctgctgc gtgtcggcct gcggcacggc
      301 gcgcatgcgt gaggatttcg tcggggcggt cggcatcaac aagatcaccc gcttccgact
      361 cgatccgcgc gacgcgcgca cggatgcgga ctattacgac gtcattggcg acgacagcgg
      421 cgtgttcggc tgcatgtcat tgctgggctg ccacgatgtc tgcccgaagg atctgccgtt
      481 gcagacgcag atcgcgttcg ttcgaaagcg gatggtcgcc gaaggcatga agtaggagct
      541 ccggagacct cgatcactgg aaaggaacgg tcatgccgcg tatagaaaaa gacttcctcg
      601 gcgagaaaca gctgcctgac actgcctact acggcgtgca gacgctgcgg ggcaaggaaa
      661 acttcaacat caccggcgtg ccgatggcga cggagcccta cttcgtcaag tccttcggct
      721 acgtcaagaa agcggccgcg ctcgccaacc gcgacctcgg ggtgctcgac gcgaaagttg
      781 cgaacgccat cgccgccgcc tgcgaccggc tgatcgccgg cgagatgcgc gaccagttcg
      841 tcaccgactt catccagggc ggcgcgggca cctcgacgaa catgaacgcg aacgaggtga
      901 tcgccaatat cgcgctcgag agcctcggcc acaggaaagg cgagtaccag tacgtcaacc
      961 cgaatgacca cgtcaacttc gggcagtcga ccaacgacat ctacccgacc gcgttccggc
     1021 tcgcgctgat cctgcggctc ggcagctaca tggaggcgct gacgaagctc caggaagcct
     1081 ttttcgcaaa gggcaaggag ttcgacaagg tgctgaagat gggccgcacg cacctgcagg
     1141 atgcagtgcc gatgtcgctc gggcaggagt tccacggctg gggcacgacc atgggcgagg
     1201 aggtgcagcg aatcgcggag gtccgccagt tcctacacga gatcaaccta ggcgccaccg
     1261 cgatcggcac taccgtgacc gccgcgccgg gttatccgga actggcgacc aagtacctgt
     1321 cggaactcac cggcttcaaa ttcatcctgg cgggtgacct gatcgaggcc acgtcggata
     1381 ccggcgcgta cgtactgctg tcgggcgtgc tgaagcgcac gtcctcgaag ctgacaaaga
     1441 tctgcaacga cctccgcatg ctcgcctcgg gaccgcgctg cgggttcaac gagatcaatc
     1501 tgccgcagat gcagccgggc tcgtcgatca tgccgggcaa ggtcaatccg gtgatcccgg
     1561 aggtggtcaa ccagaccagc ttcctcgtca tcggcctcga cctgacagtg acgctggccg
     1621 cttcagctgg ccagctacag ctcaacgtga tggagcctgt gatcacgttt gcgctcttca
     1681 cctcgattca cacgatggag aacgcggtca acagcctgcg tgagaactgt gtggtcggca
     1741 tcactgcgaa cgcagaacgc acgcgcgaca tggtgctcaa ctcgctcggc attgtgaccg
     1801 tgctcaaacc ctcgctcggc tacaagcagt gcgccgatat cgccaaggaa ggctacgaga
     1861 caggcaagcc gctgcacgac atcgtagtca aggaacgcaa gctgctaacg caggaaaagt
     1921 gggacgaggt cttctcgttc gagaacctga tcagcccgaa gttcgagcag tgaaaaccga
     1981 tccggcgcac ggagtacgcc gcggagaaag caaatgacca tctcaatcgt tctgcagttc
     2041 gtggtcgtcc tggctgcgat ctggatgggc tcgcgcttca gcggcgtcgg ccttggcctg
     2101 tggggcgcgg ttggcctact ggtgctggtc gtggccttcg gagtcagccc gacttcgccg
     2161 ccgatcgacg taatgttgat tatcctggcg gtcatcatgg ccgcctcggt gatggatgca
     2221 gccggcggta tcgatttcct ggtgcgcatt gccgagcgca tcatccgcgc gaatccaaag
     2281 tacgtgacga tcgtcgctcc cctgacgacg tggagcttca cctttgtcgc cgggactggc
     2341 cacatcgtct atccactgct gccggtcatc tacgaaaccg cacatcagag cggtgtgcgg
     2401 cccgagcggc cgatggcggt agccacgatc gcttcgcagc aggcgatcac cgcgagcccg
     2461 gtcgccgcgg ccacagccgc gatgatcggg ctgttctcag agaagggact gaccgagtgg
     2521 ggcctgcctg agatcctgat ggtgtgcgta ccggcgacgc tgaccggcgt gatcgccgcc
     2581 gcgatcgttt cgatgttcat cggcaaggac ctcaaggacg acccggaata ccaggcgcgc
     2641 ctgaaggccg gcaagattcc cgttcccatg gccgccgccg atcgcccacc gctgaaggct
     2701 tcagccaaac tgtcagcgtt cctgttcctc gcgggcgtcg cactcgtcgt gctgttcggc
     2761 ttcttccccg agttgcgcaa gctgccgggc gccaaggcgc cactcggaat gccgatcgtc
     2821 atcgagatcg tgatgatgtc ggtggccgcg atcatgctgt tgttcaccaa ggtcgatgtc
     2881 gacgaaatcc ccaagacagc cacattgcgc gccggcgtgg tggcggtcat cggcatcttc
     2941 ggccttgcat ggctcggcga cagcttcatc gcagcacaca aggatgtcat cgtgccggcc
     3001 attggcgact gggccaaggt ggcaccgtgg accttcgcgt ttggtctctt cttcgcttcg
     3061 gttctgctgt acagccaggc ggcgacgacc cgtgcgctga tgccgctcgg catggcactg
     3121 ggcatcccgc cgcagttcct cattgcgatg ttcccgtcgg tcaacggcta ctttttcatt
     3181 ccgacctacg ggtcgctgat cgcggcgatc aacttcgacc tgtccggcac gacgaagatt
     3241 ggcaagtacg tgctgaacca ctcgttcatg atcccgggca ttgttgccac cgcaacggcg
     3301 gtgataacgg gcctcttcct ggcgaagctc atgttctagt cgggggccac ggtagacgct
     3361 gttgtcggcg ccgccatgat ggacatcttc tcgccttcct cgattgaggt ctgtcatgcg
     3421 cctgctactg ctgagcaatt ccaagaccga aggcaagggc tacctggagc acgcggccgg
     3481 ctggctgcgc gacttcctgc cgacgggcgt ggatgaggtc ctgttcatcc cgtacgccgg
     3541 cgtgacgaag acgcactatg actatgtcga ccaagtagca ccggtcttcg ccagcctggg
     3601 cgcaagcgtc agcggactgc accgggcgca tgacctggtg gccgccatcc gcaacgccga
     3661 cgccatcgtg gtcggcggtg gcaacacgtg gaacctgttg cgcgagctgc gagcgcaccg
     3721 gctggtgctg ccggccatac gtcaatgcgt gctggcgggt gtgccctaca tcggctggag
     3781 cgcgggttca aacatcgcct gtcctacgat catgaccacc aacgacatgc cgatctgcga
     3841 tcccggcggt ttcgacgcgc tggcgctggt gccattccag atcaatccgc actaccagca
     3901 cggcaaccca ccagggttca agggggagac gcgcgaggag cgcattcgcg aattcggtgt
     3961 ccttcacccg gattcctggg tggccgggtt gcgcgaaggc accggttttc ttgtcgagga
     4021 ccggtcgatg cggctgctgg gcgataagga gtgccgcatc ttccgtggtg cggaagaacc
     4081 gcgcgaacgc acgtccgcag acgatttctc gttccttctg gagtaaccag tggctggtac
     4141 cgttgttgtg gcgggtagcg cgagtcgcac gcgagatctg cagagacgaa tccggcgtgt
     4201 cggtctcgtc gccggtcccc tggttgcgtt gatgttctac tggttactgc cgcacgagta
     4261 ccgcgcaacc gacggcaccg acgtggtgtt cggccacacc ggtcgcgcga cgcttgcaat
     4321 gatggggtgg atggcgacct ggtggatgac cgaagcggtc gagatcgaag tcacttccct
     4381 gctgccgatc gcgctcttcc cgatactggg cgtcatgcca ctcggcaaga cgaccgccaa
     4441 ctacggcgcg gacgtgattt atctcttcct cggcggcttc gtcattgcgc tggcgatcgc
     4501 acgctgtggg ctcgatcgac gcatcgcctt tttcacgctg cgactggtcg gcacgcggcc
     4561 gaaggcgatc gttgccggca ccatgggcgc gaccgctttc ctcagcatgt gggtatccaa
     4621 cacggcgacg gccgcgatga tgattccgat cgtgctgtcg gtgatcgatg tggcgctgcg
     4681 ccgaaggacc ggcaagggac tggccgagca cggcgggatt cccgacgatg accgcgacat
     4741 gcgcaacttc gcgctgtcgg cgctgctcgg tgtcgcctac gcggcatcga tcggcggact
     4801 gggaaccatc atcggctcgc ccccgaacgg catcctggtg cgctttatcg agcagacatc
     4861 cggaatccag gtgagctttg tgaagtggat gctgatcggc gtaccggtca tgctggcatt
     4921 cctgccactg gcgtggttcc tgaacacctc ggttcttttt ccgacgcgca tgaaggagat
     4981 cgagggcggt cgtgactacg tgcgtgacga gtgggcaaag ctgggtccct tgagccgcgg
     5041 cgagcgcgcc acgctcgccg tcttcggcat ggccgtcttc ctgtgggtga ctgcgccggc
     5101 gctgaagcag tgggagctcg gcggcatcgc gccgctcgcc ggcttgtcgg atgccggcat
     5161 cgccatcatc gcggcgatca cgctgtttct gatcacggtc gaccgcgaga ggggcgaatc
     5221 gctgatggac tggaccaccg ccgcaaagct tccgtggggc gtcctgatcc tgttcggcgg
     5281 agggctggcg ctggagtctg ccaccgaagc caatggtgtt ccgggctata tcggcagcct
     5341 tgcagcgggt ttttcgggct ggccggtgtg ggcagtgctc ctggtcatca tcgcgatcat
     5401 ggtcttcatg tcggaactga cctcgaacac cgcgcaggtc gcgacgatgc tgccgatact
     5461 ggcggcacta gcgccggtgc tcggcgtgcc gaccgcactc atgctggtac cggcgactat
     5521 cgccgcgagc tgtgcattca tgatgcc
//
LOCUS       DATIRT010000037         6533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1184307, whole genome shotgun sequence.
ACCESSION   DATIRT010000037 DATIRT010000000
VERSION     DATIRT010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6533
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184307"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VLN25_00775"
     CDS             <1..462
                     /locus_tag="VLN25_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019254388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HSQ09140.1"
                     /translation="QLGTAFAVTTEGDAHPNFKKVLAEAKPEDIVEFISVAGLPARAV
                     RTLWLERYLRRESVLQQEAADWTAKKLPPDPEGRCSGALQCLSTCGLRDGIAKIGQFC
                     IDTQLAAALRGELSRGLFFRGASSVPFGTAIRPVHELIDYLLTGKRPAAGA"
     gene            complement(467..1258)
                     /gene="fabI"
                     /locus_tag="VLN25_00780"
     CDS             complement(467..1258)
                     /gene="fabI"
                     /locus_tag="VLN25_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HSQ09141.1"
                     /translation="MGFLSGKRLLITGVLSNRSIAYGIAQACKREGAELAFTYVGDRF
                     KERIIEIAAEFGSDLVFDMDVADDAQINAAFDALEQRWPALDGLVHSIGFAPREAIAG
                     DFVDGLSREGFRIAHDISAYSFPALAKAALPLMEGRQAALLTLTYLGAERAVPSYNTM
                     GLAKASLEASVRYLAASLGPRGIRVNGISAGPIKTLAAAGIKGFSKIFSVVESTAPMR
                     RNVSIEDVGNVAAFLLSDLAAGITAEITHVDCGFNAVMGIPTGLE"
     gene            complement(1349..2941)
                     /locus_tag="VLN25_00785"
     CDS             complement(1349..2941)
                     /locus_tag="VLN25_00785"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HSQ09142.1"
                     /translation="MRIALSLLSAVLACGFATASAVEPAPGAPESRSASVATPAAGIA
                     APSTAATYDIPLDVILEYMPELAPADSTAVLTAPLVAPPSIDLWQRIRAGFRMPDLDT
                     KLADQRTRWYAEQVDYFARMTQRSSRYLYFIVEEIERRGMPTELALLPFVESAFKAEA
                     VSTAKAAGLWQFIPSTGRDYDLEQNMWKDERRSVIDSTRAALDYLQKLHDMFGDWHLA
                     LAAYNWGEGSVSRAIEKNRRVGKPGGYTSLKMPLETQHYVPKLQAVKNIVSEPEKFGI
                     ELPPVPNSPYFVAVRKTRDIDVQTAAKLAEMSPDEFQALNPQFNRPVIVGAASPTLLL
                     PANRLDTFNANLAAWEATGQPLASWTTYRMKPADTLATVSQRAGIAEQQLREANRIPP
                     RYRLAPGSTILVPRDETMEDITAANLDARFALVPEASNLRKVTYRVRRGDTLYSVARR
                     YRVNEKDVIVWNQLTSPTLFAGQRLEFTVPVAKSNAVAKGRKPGAKQEAQRKPAPVKT
                     AAKAPARPGTNGTNGKVVAASR"
     gene            complement(3013..3804)
                     /gene="gloB"
                     /locus_tag="VLN25_00790"
     CDS             complement(3013..3804)
                     /gene="gloB"
                     /locus_tag="VLN25_00790"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HSQ09143.1"
                     /translation="MTRVATPSVSITPLPAFSDNYLWLLERAGHAVVVDPGDAAPVQR
                     ALAERKLKLDAILVTHHHGDHVGGLAALRTTGATVYGPRAEAIDGVDVPLAAGDRVEV
                     LGVSFSVLEVPGHTSGHIAYFAGALEPPVLFCGDTLFACGCGRLFEGTPAQMLASLDR
                     LSALPAATRVYCAHEYTLANIRFALAVEPANAELQQRARDAALVRERGEPTVPSTIAL
                     ELATNPFMRSDAPGVRNAAGARTASAGTDRVRTFTTIRKWKDEFR"
     gene            3817..4677
                     /locus_tag="VLN25_00795"
     CDS             3817..4677
                     /locus_tag="VLN25_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSQ09144.1"
                     /translation="MNSSGSIVKLSEFLCSPAGRYVLGWEQQQLDAAVTDIFGYHALQ
                     LGLPEIDALRENRMPLRFCAADRQLTIPAQDGAAEGGERRVAIINRYEELPFATHSID
                     LVVMPHILEFAADPHQVLREVERVLVPEGHVVITGFNPASLWGLRQYSTRLGASPFLP
                     REGRFISLPRVKDWLKLLSFDFARGRFGCYAPSARTAAWLNRWRFMEKAGDRWWPVFG
                     AVYLLTAVKRVRGMRLLGTVWKHSEERARRLTPVATTRVALTTPDARSAIVLDDEAAA
                     NDARLRARRG"
     gene            4674..5174
                     /gene="rnhA"
                     /locus_tag="VLN25_00800"
     CDS             4674..5174
                     /gene="rnhA"
                     /locus_tag="VLN25_00800"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HSQ09145.1"
                     /translation="MKHAKSVALTSVQAKSTDDVEVEIWTDGACKGNPGPGGWGALLK
                     YGGHERELFGGESPTTNNRMELMAVIEALGSLKRGCRVVLHTDSQYVQLGITEWLPNW
                     IRRDWKTAAKKPVKNADLWQLLQAAAARHDIDWRWIKGHAGHEGNERADELANRGVKS
                     VSMSAD"
     gene            5246..6490
                     /locus_tag="VLN25_00805"
     CDS             5246..6490
                     /locus_tag="VLN25_00805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSQ09146.1"
                     /translation="MKHRVTIIAIGVLIAVAVAAYFARDLMSPGPNAVAGVKVDAKTD
                     AKAAGQSDGKGGATKGASGGPTPVEVVSLAPTVVKEDLQAVGSLRSNESVILRPEVSG
                     RIATIGFKDGQLVRKGQLLVGLDATLNEAEVYQARAEYDLALSNLKRSEDLASRKFIS
                     SSAQETATSNAQVAEAKLKLAQARLSKMRIVAPFDGSVGIRKVSLGDYVKDGTDLVNL
                     EDVRILKVDFRLPERNISQIRVGQTIEVVADALPNEHWQGRIEAINPKVDADGRSLEL
                     RALLENTSGKLRPGMFVRVRVIVGERSNALLVPEEVIVPQGSEFYVFKVVDGAARRVP
                     VKIGVRRDAMVEILDGLAVGDQVVTAGMRLSRDGQPVRVLAPGQGVPPKGGTEIGKDA
                     APKAGAVPGAPKADVNKGSGQS"
BASE COUNT         1114 a         2038 c         2262 g         1119 t
ORIGIN      
        1 cagctgggca ctgccttcgc ggtgaccacg gaaggcgacg cgcatccgaa cttcaagaag
       61 gtgctcgccg aagcgaagcc ggaagacatc gtcgagttca tcagcgtcgc cggcctgccg
      121 gcccgtgcgg tgcgcacgct ctggctggaa cgctacctgc gccgcgaatc cgtgctgcag
      181 caggaggccg cggactggac cgcaaagaag ttgccaccgg atcccgaggg ccgttgcagt
      241 ggagcgctgc aatgcctttc gacgtgcggg ctgcgcgatg gcatcgcaaa gatcgggcag
      301 ttctgcatag acacccagct tgcggccgcg ctgcgcggcg aactgtcgcg tgggctgttt
      361 ttccgcggag cgtcgtcagt gccgttcggc accgcaatcc ggccggtgca cgagctgatc
      421 gattatttgc tgacgggtaa gaggccggca gccggcgcct gacgacctac tcgaggccgg
      481 tggggatacc catcacggcg ttgaatccgc agtcgacatg cgtgatctcg gcggtgattc
      541 cagcggcgag gtcggacagc aggaacgccg cgacattgcc gacatcctca atcgaaacat
      601 tgcgccgcat cggcgcggtg ctctcgacca ccgagaagat cttcgaaaac cccttaatgc
      661 ccgccgcagc cagcgtcttg atcgggccgg ccgaaatgcc gttcacacgg atgccgcgcg
      721 ggccgaggct ggcggccaga tagcgcacgc tcgcctcgag actcgcctta gccagcccca
      781 tcgtgttgta gctcggcact gcgcgctcgg cgccgaggta ggtcagcgtc agcagggccg
      841 cctggcgtcc ttccatcagc ggcagtgccg ccttcgctag cgccgggaaa ctgtaggccg
      901 agatgtcgtg cgcgatgcga aaaccctcgc gtgacagacc gtcgacgaag tcgcccgcaa
      961 tggcttcgcg cggggcgaag ccgatcgagt gcacgaggcc atcgagcgcg ggccagcgct
     1021 gctccagcgc gtcgaacgct gcgttgatct gagcgtcgtc ggcgacgtcc atgtcgaaca
     1081 cgagatcgga gccgaattcg gcggcgattt cgatgattcg ctccttgaac cggtcaccta
     1141 cataggtaaa cgcgagctcg gcgccctcgc gcttgcacgc ctgcgcgatg ccataggcga
     1201 tcgatcgatt ggacaacaca ccggtgatca gcagccgctt gccggacaga aagcccatgg
     1261 tcttggatgc gtaggaaggg tttgagtcgg gcgcgcagta taggcgctga accgtgcccg
     1321 gcccttcgcc acggcacgct cgcgagcgtc agcgcgaggc cgcgacgacc ttgccgttgg
     1381 tgccgttagt gccgggacgc gcgggcgcct ttgccgccgt cttaacgggg gcgggcttgc
     1441 gctgagcctc ttgcttcgca cctggcttgc gtcccttcgc cacggcgttc gacttggcga
     1501 ccgggacggt gaactcgagc cgctggcctg cgaacagcgt tggcgatgtc agttgattcc
     1561 agacgatcac atccttttca ttgacgcggt agcgacgggc caccgaataa agcgtgtcac
     1621 cgcggcgcac gcggtaggtc accttgcgca ggttcgaggc ctcgggcaca agcgcgaacc
     1681 gtgcatcgag gttcgcggcc gtgatgtctt ccatcgtctc atcgcgcggg accaggatcg
     1741 tcgagccggg cgcaagccga tagcgcggcg gaatgcgatt ggcctcgcgc aactgctgtt
     1801 cggcgatgcc ggcgcgctgt gacaccgtgg cgagcgtgtc ggcgggcttc attcgatacg
     1861 ttgtccagct ggccagcggc tggccggtgg cttcccacgc ggccaggttc gcattgaagg
     1921 tgtcgaggcg attggccggc agcagcagcg tgggtgacgc cgcgccgacg atcaccggcc
     1981 ggttgaactg cgggttgagc gcctgaaact cgtccggcga catttccgcc agtttggccg
     2041 ccgtttgcac gtcgatgtcg cgcgtcttcc gcacagcgac gaagtagggc gagttcggaa
     2101 ccggaggaag ctcgatgccg aatttttcag gctcggagac gatgttcttc accgcctgta
     2161 gcttgggaac gtagtgctgc gtctcgagcg gcatcttgag gctggtgtag ccgcccggct
     2221 tgccgacacg ccggttcttc tcgattgcgc gcgacactga gccttcgccc cagttgtaag
     2281 ccgccagcgc gaggtgccag tcgccgaaca tgtcgtgcag cttctgcaga taatcgagcg
     2341 ctgcccgcgt ggagtcgatc acggagcggc gttcgtcctt ccacatgttc tgctcgaggt
     2401 cgtagtcccg gccggtggat ggaatgaact gccacaggcc ggccgccttg gcggtcgaaa
     2461 ccgcttcggc cttgaaggcg ctctcgacga acggcagcaa tgcgagctca gtcggcatgc
     2521 cgcggcgttc gatctcttcg acgatgaagt acaggtaccg gctcgatcgc tgtgtcatgc
     2581 gcgcgaagta gtcgacctgt tcggcgtacc agcgtgtgcg ctggtcggcg agttttgtgt
     2641 cgaggtccgg catccggaag cctgcgcgga tgcgctgcca caagtcgatc gagggcggcg
     2701 cgaccagcgg agccgtcagg acggccgtgg aatctgccgg cgccagctcc ggcatgtact
     2761 cgaggatcac gtcgagcgga atgtcatagg tcgccgcagt cgagggtgcc gcgattccgg
     2821 ccgcaggggt cgcgaccgat gccgagcggg attcgggtgc gccgggcgca ggctcgaccg
     2881 cactcgccgt ggcgaagccg caggcgagca ccgcagagag cagggaaagc gctatgcgca
     2941 tggttctcga ggaaaggggg accggcagca gtgtaggcaa ccgtctatgc ggggacaagt
     3001 cgaacaaccg ggttagcgga actcatcttt ccatttccgg attgtggtga atgtgcgcac
     3061 gcggtccgtc cctgcgctgg ccgtgcgtgc gcccgctgca ttccggacac ccggcgcatc
     3121 gctgcgcatg aacgggttcg tggcgagttc cagcgcaatc gtcgacggca ctgtcggctc
     3181 tccccgttcc cgcaccagcg ccgcatcgcg tgcgcgctgt tgcagttcgg cgttggccgg
     3241 ttcgaccgcg agcgcaaagc ggatgttggc cagcgtgtac tcgtgggcac agtacacgcg
     3301 cgttgccgcc ggcagtgcgg acagacggtc gagcgaggcc agcatctgcg cgggcgttcc
     3361 ctcgaagagt cgtccgcagc cacaggcgaa gagcgtgtcg ccgcagaaaa ggaccggtgg
     3421 ctcaagtgca ccggcgaagt acgcgatgtg tcccgatgtg tggccgggta cttcgaggac
     3481 actgaacgac acgccgagca cctcgacacg atcgcccgcc gctagcggga cgtcgacgcc
     3541 gtcgatggct tcggcgcgcg gtccgtagac cgtggcgcca gtcgttcgca gtgcggccag
     3601 gccgcccaca tggtcgccgt ggtgatgggt gactagaatc gcgtccagtt tgagcttacg
     3661 ttcggccagc gcgcgctgga ccggcgcggc atcgccgggg tcgaccacga cggcgtgtcc
     3721 agcgcgttcg agcaaccaca ggtagttgtc gctgaacgcg ggcagcggag tgatcgaaac
     3781 ggacggcgtg gcaactcggg tcatggcaga gagcagatga attccagcgg ttcgattgtg
     3841 aagctatccg agttcctctg ctcgccggcc ggacgctatg tgctcggctg ggagcagcag
     3901 cagctcgatg cggccgtgac cgacatcttc ggctatcacg cgttgcagct cgggctgccg
     3961 gagatcgatg cgctgcggga gaaccgcatg ccattgcgct tctgcgcggc ggatcggcag
     4021 ctcaccatcc cggcgcagga cggcgcggcc gaaggcggcg agcggcgcgt ggccatcatc
     4081 aatcgctacg aggagttgcc gtttgcgacg cactcgatcg acttggtcgt aatgccgcat
     4141 atcttggagt tcgcggccga tccgcaccag gtgctgcgcg aggtcgagcg cgtgctcgtg
     4201 ccggaaggcc acgttgtcat cacgggcttc aatcccgcca gtctgtgggg actgcgccag
     4261 tacagcacgc gcctcggcgc gtcaccgttc ctgccgcgcg aaggccgttt catctcgctg
     4321 ccgcgcgtga aggactggct caagctgctg tcattcgatt tcgcgcgcgg ccgcttcggt
     4381 tgctatgccc cgtcggcacg cacggccgcc tggctcaatc gctggcgctt catggagaaa
     4441 gccggggatc gatggtggcc ggtgttcggt gccgtctatt tgctgacggc cgtgaagcgc
     4501 gtgcgcggca tgcggctcct cggcacggtg tggaagcaca gtgaagagcg tgcgcggcga
     4561 ctgacgccgg tggcgaccac gcgcgtggcg ctgacgacac ccgacgcgcg gtctgccatc
     4621 gtgctcgacg atgaagccgc agccaacgat gcgcggctgc gcgcgcgacg cggatgaagc
     4681 acgcgaagag cgttgcgctg acctcggtgc aggccaagtc gactgatgac gtcgaggtcg
     4741 aaatctggac cgacggcgcc tgcaagggca atcccgggcc cggcggctgg ggcgcgctgc
     4801 tgaagtacgg tggtcacgag cgcgagctct ttggcggcga gtcaccgacc accaacaacc
     4861 gcatggaact gatggcagtg attgaggcgc tgggttccct caagcgcggc tgccgcgtcg
     4921 tgctgcatac cgactcgcag tacgtgcagc tcggcatcac cgagtggctg cccaactgga
     4981 tccggcgaga ctggaagacg gcggctaaaa agccggtgaa aaatgcggac ctgtggcagc
     5041 tcctgcaggc tgccgccgcc cgccacgaca tcgactggcg ctggatcaag gggcatgccg
     5101 gccacgaggg taacgaacgg gccgacgaac tggcgaaccg cggggtcaaa tcggtgtcta
     5161 tgtcggctga ctgactctgt atggcccgag ggcgggtgtt aaagtcgcgc ttcgcttgcg
     5221 cgccctcttt ttccagatct ttccaatgaa gcatcgagtc acgatcattg cgatcggggt
     5281 actgatcgcc gtcgccgttg ccgcctattt cgcgcgcgac ttgatgtcgc ccggccccaa
     5341 tgccgtggcc ggcgtgaaag tcgatgccaa gaccgacgcc aaggcggcag gccagtcgga
     5401 cggcaagggc ggcgcgacca aaggcgcttc cgggggaccg acgcccgtcg aggtagtgag
     5461 cctcgccccg acggtggtca aggaagacct gcaggccgtc ggctcgctgc gttcgaacga
     5521 aagcgtgatc ctgcgtccgg aggtgtcggg ccgcattgcc accatcggtt tcaaggatgg
     5581 gcagctcgtg cgcaagggtc agctgctggt cgggctcgat gcgacgctca atgaagccga
     5641 ggtctaccag gccagggcgg agtacgacct ggcgctgtcg aacctcaagc gcagcgagga
     5701 tctcgccagc cgcaagttca tcagctcgag tgctcaggag accgccacct cgaacgcgca
     5761 ggtggcggaa gccaagctaa agctggcgca ggcccgactt tccaagatgc gcatcgttgc
     5821 gccgttcgac ggatcggtcg gcatccgcaa agtcagcctc ggcgactacg tcaaggacgg
     5881 taccgacctc gtgaacctcg aggatgtccg cattctgaag gtcgactttc gcctgccgga
     5941 gcgcaacatt tcgcagatcc gcgtgggcca gacgatcgag gtcgtcgccg acgcgcttcc
     6001 caatgaacac tggcaaggcc gcatcgaagc gatcaacccg aaggtggatg ccgacggccg
     6061 ctccctggag ctccgcgcac tgctcgagaa caccagcggc aagttgcggc cgggaatgtt
     6121 cgtgcgcgtg cgcgtgatcg ttggcgagcg gtcgaacgcg ctgctggtcc ccgaggaagt
     6181 aatcgtgccg caaggctccg agttctatgt gttcaaggtc gtcgacggcg cggcccggcg
     6241 cgtgcccgtg aagatcggcg tgcgacgcga tgcgatggtc gagatcctcg acggcctcgc
     6301 cgtcggtgac caggtcgtga cggccggcat gcggctgtcg cgcgacggcc agccggtgcg
     6361 cgtgctggcg ccggggcagg gcgtaccgcc caagggcggc accgagatcg gcaaggacgc
     6421 ggcgccgaag gcgggcgcag tgcccggtgc gccgaaagcc gacgtgaaca aaggaagcgg
     6481 tcagtcctga gcagttgatc acgggagaat gcgatgagcc tgttcgagct gtg
//
LOCUS       DATIRT010000038         2750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1381107, whole genome shotgun sequence.
ACCESSION   DATIRT010000038 DATIRT010000000
VERSION     DATIRT010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381107"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VLN25_00810"
     CDS             complement(<1..839)
                     /locus_tag="VLN25_00810"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HSQ09147.1"
                     /translation="MDPRAMSGAELDAATALARLSQSIDPISELEQCPTGAALRRVLG
                     ADVMATLDLPPFDNAAMDGYAVRSIDCTGASTLRVAGRALAGHPFTGQVHPGEAVRIM
                     TGAALPAGADAVVAQENVQVRGDQVEILSGVPPATHVRPRGQHLQDGALVLARGRRLR
                     DYDLGLAAAAGSAELTVVRRLRVGVLSTGDELHDAPHGRGPGGQYDGNRPMLLAALES
                     AGYQAIDLGIVADQADALSEALDGAARKHLDAVVSSGGVAQGDADIVRRFPGLEFVPL
                     AIRP"
     gene            complement(877..1995)
                     /gene="moaA"
                     /locus_tag="VLN25_00815"
     CDS             complement(877..1995)
                     /gene="moaA"
                     /locus_tag="VLN25_00815"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HSQ09148.1"
                     /translation="MTDRIIQLLDLRAARLPVPLAGSTVADGQVVDRLARPLRDLRIS
                     VTDRCNFRCTYCMPKEIFDRDYRFLQHAELLSFEEITRLTRAFVDNGVEKVRLTGGEP
                     LLRKDLQRLVEMLARLTTRDGRPLDIALTTNGSLLPRKARVLANAGLSRVTVSLDGLD
                     DTVFKRMNDVEFPVSDVLAGIDAAAAAGLGPVKINMVVKRGVNDQEVVAMARHFRGTG
                     HIVRFIEFMDVGSSNGWRMDDVVPSATVIERIDREVAPVEAIDPNYPGEVAERWRYRD
                     GSGEVGVISSVTQAFCRSCTRARLSTEGMLYLCLFADRGYDLRRLLRGAPELPAFEDP
                     ALAATIAQIWQQRGDRYSELRSEATAQRPKVEMSYIGG"
     gene            complement(2078..>2750)
                     /locus_tag="VLN25_00820"
     CDS             complement(2078..>2750)
                     /locus_tag="VLN25_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09149.1"
                     /translation="DISPISPVPASASLVTPDAATADATSDEHRGKRRRRRRGRGGRG
                     GEGEATSGVEVSERDVDEDLAEAAHTVPESVEEAVMPSAAVAVSGGNGSAIAQPETTM
                     TSVEARSAAPEPTERERPREAVPVAHVAVAAVTPQSIVVPTTFSLPVAALAPMPVEHL
                     DSVLQMAGLTLVQTEPAKLEHTRARMATEPRPPRLPRERPVQLPLDTDPLVQVETRVA
                     QDRPS"
BASE COUNT          471 a          933 c          913 g          433 t
ORIGIN      
        1 gggcggattg ccagaggaac gaattccagc ccggggaaac ggcgaacgat gtcggcatcg
       61 ccctgggcca cgccaccgct cgacaccacg gcatcgaggt gttttcgcgc ggcgccgtcc
      121 agcgcctcgg acagggcgtc agcctggtcc gcgacgatgc cgaggtcgat ggcctgatac
      181 ccggcagatt ccagcgctgc cagcagcatc ggccggttgc cgtcgtactg tcctcccgga
      241 cctcgcccgt ggggagcatc gtgcagttca tcgccggtcg aaagaacgcc aacgcgcaac
      301 ctgcgcacga ccgtcagttc ggcggaaccg gcggcagccg caaggccgag gtcatagtcg
      361 cgcagccggc gcccgcgcgc gaggacgagg gcgccgtctt gaaggtgctg gccgcgtggt
      421 cgcacgtggg tagcgggcgg gacgccggac agaatctcca cctggtcgcc gcggacctgc
      481 acgttctcct gggcgacgac ggcgtccgca ccggcgggca aggccgcgcc cgtcatgatg
      541 cgcaccgcct ctcccgggtg cacttggcct gtaaagggat ggccggccag cgcgcgtccc
      601 gccacacgca gcgtggatgc tccggtgcaa tcgatcgagc gcaccgcata tccgtccatc
      661 gccgcgttgt cgaacggtgg caggtcgagc gtcgccatga catccgcgcc tagcacccgg
      721 cggagggctg cacccgtggg gcactgctcg agctcgctga tcggatcgat ggactggctg
      781 agtcgggcaa gagcggtcgc cgcgtcgagt tcagccccgc tcatcgcgcg agggtccatc
      841 caaggaggca gtcgtgcgcg gcctgcggca tcgcactcag ccgccgatgt aggacatctc
      901 gaccttcggg cgttgcgcag tcgcttcgga gcgcaactcg gaatagcgat caccacgctg
      961 ctgccagatc tgcgcgatgg ttgcggccag tgcgggatcc tcaaaggcag gcagttccgg
     1021 tgccccgcgc agcagacggc gcaggtcata gccgcggtca gcgaacaggc acaggtacag
     1081 catgccttcg gtcgaaagcc tcgcgcgcgt gcagctgcgg cagaaggcct gcgtcacgct
     1141 cgagatgacg cccacttcac cactgccgtc gcgataccgc caacgctcgg ccacctcgcc
     1201 cggatagttc gggtcgatcg cctccaccgg cgccacttca cgatcaatgc gctcgatcac
     1261 tgtcgcggaa ggcacgacgt catccatgcg ccagccgttg gaactgccga catccatgaa
     1321 ctcgatgaag cggacgatgt gcccggtgcc gcgaaagtgc cgcgccatgg caacgacttc
     1381 ctggtcgttg acgccgcgct tcacgaccat gttgatcttg accggaccga ggccggcagc
     1441 cgccgcagcg tcgatccccg caaggacatc ggagaccgga aactcgacat cattcatgcg
     1501 cttgaagacg gtatcgtcaa ggccgtccag cgacaccgtg actcgactga gtccggcatt
     1561 ggccaggaca cgtgccttgc gaggcagcag ggatccattc gtggtgagtg cgatatcaag
     1621 cggacgcccg tcgcgcgtgg tcagccgcgc caacatctcg accagccgtt gaaggtcttt
     1681 gcgcagcagc ggctcgccgc cggtcagacg caccttttca acgccgttgt cgacgaaggc
     1741 gcgagtcagt cgcgtgatct cctcgaaact cagcagctcc gcgtgctgca ggaaccggta
     1801 atcccgatca aagatctcct tcggcatgca ataggtgcag cggaagttgc atcgatccgt
     1861 gaccgagatc cgcaggtcgc gcagtggacg cgccaaccgg tcgaccacct ggccatccgc
     1921 cactgtgctg cccgcgagcg gaacgggcag ccgcgccgca cgcaggtcaa gcagctggat
     1981 gatgcgatcg gtcatgcgtc ggatgttacc ggcgctggca gtcagcccat ggcgcagcgc
     2041 ggcacgtttc cgaagaatcg tgccgcacgt tcgaccctta cgacgggcga tcctgcgcta
     2101 cgcgcgtttc aacttgcacc aggggatcgg tgtcgagggg cagctggacc gggcgctcac
     2161 gcggtaaccg cggtgggcgc ggctcggtgg ccatgcgggc gcgagtgtgc tcgagcttcg
     2221 cgggctcggt ctgcaccagc gtcagacccg ccatctgcag cacggaatcg aggtgttcca
     2281 ccggcatggg cgctaacgcg gcaaccggca gggagaacgt tgtcggcacc acgatcgact
     2341 gcggagtgac cgcagcgact gcgacatgcg ccacggggac ggcttcgcgc ggtctttccc
     2401 tttcggtggg ctcaggagca gcggaccggg cctcgaccga cgtcatcgtg gtttctggtt
     2461 gcgcaatggc gctgccgttg ccgccggaca cggcgacggc cgcgctcggc atgaccgcct
     2521 cctcgacaga ctccggcacg gtgtgcgccg cttccgcgag gtcttcatca acgtcgcgtt
     2581 ccgaaacctc gacaccggac gttgcctcgc cttcgccacc acggccacca cgaccgcggc
     2641 gacgccggcg ccgcttgcca cgatgctcat ccgaggttgc gtcggcagtg gccgcgtcgg
     2701 gcgtgaccaa agaggcactc gcagggaccg gactgatcgg actaatgtcg
//
LOCUS       DATIRT010000039         4274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1676161, whole genome shotgun sequence.
ACCESSION   DATIRT010000039 DATIRT010000000
VERSION     DATIRT010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676161"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="VLN25_00825"
     CDS             complement(<1..768)
                     /locus_tag="VLN25_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HSQ09150.1"
                     /translation="MISIIRRQAATLAILAVTGLAAPTSVIAQQDAQKAAPAAEAPAP
                     AVAAPAPAPVAPMAAPVPTVTKETIENPYGLEALWRQGDWVSKGTLIILVIMSMGSWY
                     IIFVKLWEQFKLMRHGREMASSFWKSASLKDGAKKLKDGSAFRYIAETGLAANEHHEG
                     ALTENIDLNTWVTMSVQRAVDTVQSRQQDGLAFLATVGSTAPFVGLFGTVWGIYHALT
                     AIGIAGQASIDKVAGPVGEALIMTAIGLAVAVPAVMGY"
     gene            complement(863..1507)
                     /locus_tag="VLN25_00830"
     CDS             complement(863..1507)
                     /locus_tag="VLN25_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011351522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HSQ09151.1"
                     /translation="MDYAQQQRNPGRHVVGITAVVLLHVLIVYALVTGLARTVVEVIK
                     GPIEVKVIEEIIKKPPPPPEVVPPPPKVAAPPPPFIPPPEVNIAPPPTPAPAISAVTQ
                     EAPAGPQAPIIQKPAETPAPAPAIRSARVTCANYIEVMQSIVYPREALKDNVEGEVVI
                     EFTVTSSDQVKDTVIKSSTNRVFNRASMAVVSQLSCQGQGQDVRVQAPISFKLR"
     gene            complement(1653..2714)
                     /gene="ruvB"
                     /locus_tag="VLN25_00835"
     CDS             complement(1653..2714)
                     /gene="ruvB"
                     /locus_tag="VLN25_00835"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HSQ09152.1"
                     /translation="MVATRAETSLIEAERLVAGSPASPNEEAVERALRPGSLAEYVGQ
                     PKIREQLDIFITAARNRSESLDHVLLFGPPGLGKTTLAHIIAREMGVNLRQTSGPVLE
                     RPGDLAAMLTNLEKHDVLFIDEIHRLSPVVEEILYPALEDFQIDIMIGEGPAARSIKI
                     DLPPFTLVGATTRAGMLTNPLRDRFGIVARLEFYSTEELTSIVTRSSRLLNARASAEG
                     AREIARRSRGTPRVANRLLRRVRDFAEVKGSGEISAEVADAALAMLEVDRLGLDVMDR
                     KLLLAVIDKFAGGPVGIENLAAAVGEERDTLEDVVEPYLIQQGMLQRTPRGRVAALAA
                     YRHFERVEPRQDGSVGELL"
     gene            complement(2721..3299)
                     /gene="ruvA"
                     /locus_tag="VLN25_00840"
     CDS             complement(2721..3299)
                     /gene="ruvA"
                     /locus_tag="VLN25_00840"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HSQ09153.1"
                     /translation="MIGRLEGRLLEKSPPTLLVDVGGVGYEVDVPMSTFYQLPAISER
                     VVLLTHLAIREDAHQLYGFASAAERMAFRELIKISGVGARTALSVLSGLSVTELAQAI
                     TLQESGRLTRIPGIGKKTAERLLLELKGKLGAELGSSPSAIAGDNSADVMRALLALGY
                     NEREANAAVRQLPAGAGTADGIRLALKSLARG"
     gene            complement(3296..3874)
                     /gene="ruvC"
                     /locus_tag="VLN25_00845"
     CDS             complement(3296..3874)
                     /gene="ruvC"
                     /locus_tag="VLN25_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HSQ09154.1"
                     /translation="MPPSLRSLGIDPGLNRTGFGVIEVSGSRLGPVAAGVIRVPPGDL
                     HERLGTILRALGDVIRETRPHVATIEKVFVNVNPQSTLLLGQARGAAICAAVGEGLVV
                     HEYAALRIKQAVVGFGQADKKQVQLMVQRLLALDAVPATDAADALACAICHAHSRTLL
                     AVVTRTGTSAAPRRGSATASRRAWAARAAGGR"
BASE COUNT          718 a         1450 c         1382 g          724 t
ORIGIN      
        1 gtagcccatg accgccggta ccgcgaccgc caggccgatg gccgtcatga tcagcgcctc
       61 gcccaccgga cccgcgacct tgtcgatcga cgcctgaccg gcgatgccga tggcggtcag
      121 tgcgtgatag atgccccaca ccgtcccgaa cagtcccacg aacggcgcgg tcgatccgac
      181 cgtcgcgagg aaggcgagtc cgtcctgctg acggctctgc acggtgtcga ccgcacgctg
      241 cacggacatc gtgacccagg tgttcaggtc gatattctcg gtgagcgcac cttcgtggtg
      301 ctcgttcgcg gccagtccgg tttcggcgat gtagcggaaa gcgctcccgt ccttgagctt
      361 cttcgcgccg tccttgagcg aagccgactt ccagaacgaa ctggccattt cgcggccatg
      421 gcgcatcagc ttgaactgct cccagagctt gacgaagatg atgtaccagc tgcccatcga
      481 catgatgacg aggatgatca gcgtgccctt ggacacccaa tcgccctgac gccacagggc
      541 ctcgaggccg tacgggttct caatggtttc cttagtgacc gtcggtaccg gcgcagccat
      601 gggcgcaacg ggggccgggg ccggtgctgc tacggcaggt gccggcgctt cggctgccgg
      661 tgcagccttc tgagcgtcct gttgggcgat cacggaagtg ggtgcggcta agcctgtaac
      721 tgccagaatc gccagggtgg cggcttggcg acggatgatc gagatcattg ctaaatctcc
      781 tggtggaaga tggttcaaag gtgagaaagg agagtcacgc ggcgcggtgg cgccagggtc
      841 cgatcgcctt tgtccctgcc ggttaacgaa gcttgaaact gatcggggcc tgcacccgta
      901 cgtcctgccc ttgcccctgg caggacagct gagaaacgac cgccatcgac gcgcggttaa
      961 agacgcgatt cgtcgacgac ttgatcactg tgtccttgac ctgatcgctc gaggtcaccg
     1021 tgaactcgat caccacttcg ccttcaacgt tgtccttcag cgcttcgcgc ggatagacga
     1081 tcgattgcat gacttcgatg taattggcgc aggtcacgcg agcactgcga atcgctgggg
     1141 ccggagccgg cgtttcggcc ggcttctgga tgattggagc ctgcggaccg gccggcgctt
     1201 cctgcgtgac cgctgaaatg gccggcgctg gagtcggcgg cggcgcgata ttgacttccg
     1261 gcggcgggat gaacggcggc ggcggcgcag ctaccttcgg cggcggcggg acgacttccg
     1321 gcggcggcgg cggcttcttg atgatctcct cgatcacctt gacctcgatc ggccctttga
     1381 tcacctcgac gaccgtgcga gccaggccgg tgaccagcgc ataaacgatc aggacgtgca
     1441 gcagcacgac cgcagtgatc cccaccacgt gtcggcccgg attgcgctgc tgctgtgcgt
     1501 agtccatgaa gcccctcctg ggctcgcccc ggcgaaaagc ccgcgattct aatgctgacc
     1561 gctgcgcttt cgcaaagcgc agaattctag agcaaagggc atgccgcgga caccacaccg
     1621 cggtgcaaca aggaacgctt gtcaggattt ggctagagta gctcgcctac gctgccgtct
     1681 tgccgcggct cgacccgctc gaagtgccgg tatgccgcca atgccgcgac ccggccccgc
     1741 ggggtccgct gcagcatgcc ctgctggatc aggtacggct cgacgacatc ttccagcgtg
     1801 tcgcgctctt caccgacggc cgcggcgagg ttttcaatgc cgacaggccc accggcgaac
     1861 ttgtcgatga ccgcaagcag caacttgcgg tccatcacat ccagcccgag ccggtccact
     1921 tcgagcatcg ccaatgcagc atcggcgacc tcggctgaaa tctcgccgct gcccttgacc
     1981 tccgcgaagt ctcggacccg ccgcagcagc cggttggcca cgcgcggcgt gccgcgcgac
     2041 cgcctggcga tctcgcgggc accctccgcg ctggcacggg cattcagcag ccgcgacgag
     2101 cgcgtgacga tcgaggtcag ttcttcggtt gaatagaact ccagccgcgc gacgatgccg
     2161 aaccggtcac gcagcggatt ggtgagcatg ccggcgcggg tcgtggcccc aaccagcgtg
     2221 aacggtggca ggtcgatctt tatcgagcga gccgccgggc cttcgccgat catgatgtcg
     2281 atctggaaat cctccagcgc cggataaaga atctcctcga ccaccggcga caggcgatgg
     2341 atctcgtcga tgaaaagcac gtcgtgcttc tcgaggttcg tcagcatcgc agcgaggtcg
     2401 ccgggccgct ccagcaccgg cccggaagtc tggcgcaggt tcacgcccat ctcgcgcgcg
     2461 atgatgtgcg ccagcgtcgt cttgccgaga cccggcgggc cgaacagcag cacgtgatcg
     2521 agcgattcgg accgattgcg ggccgccgtg atgaagatgt cgagctgctc gcggatcttc
     2581 ggctgaccga catactcggc cagcgagccc ggtcgcagcg cgcgctcgac cgcttcctcg
     2641 ttcggcgacg cgggactgcc agcgaccagg cgttctgctt cgatcaacga cgtctccgcg
     2701 cgtgtggcca ccatcggccg tcagcctctg gccagcgatt ttagggccag gcgtatgccg
     2761 tcggcggtgc cggcgccggc cggcaactgt cgcaccgccg cgtttgcctc gcgctcgttg
     2821 taacccagcg ccagcagggc gcgcatcaca tctgcgctgt tgtcgccggc gatggccgac
     2881 ggcgacgaac ccagctcggc gccgagcttg cccttgagct cgagcagaag ccgctcggcg
     2941 gtcttcttgc cgatgcccgg aatgcgcgtc aggcgtccac tttcctgcag cgtgatcgcc
     3001 tgtgccagtt ccgtcacgga cagtccggac aggaccgaca gcgcggtgcg ggcaccgacg
     3061 ccggaaatct tgatcagttc gcgaaacgcc atgcgttcgg cggcgctggc aaacccgtag
     3121 agctggtgcg catcctcgcg aatggcgagg tgcgtcagca gcacgacccg ctcggagatc
     3181 gccggcaact gatagaacgt gctcatcggc acatcgacct cgtagccgac acccccgaca
     3241 tcgaccagca gtgtcggggg gctcttttcc agcaaccggc cttcaagacg accgatcatc
     3301 ggccgcccgc tgcccgcgcc gcccaggcgc gccgcgaggc agtggccgac ccccgccgcg
     3361 gcgcagcgct cgttcccgta cgcgtcacca ctgccagcag cgtgcgcgaa tgcgcatggc
     3421 agatcgcgca cgcaagcgca tccgccgcat cggtcgcggg gacagcgtcg agtgcgagca
     3481 ggcgctgcac catcaactgg acctgcttct tgtcggcctg gccaaagccg acgaccgcct
     3541 gcttgatccg cagcgcggcg tactcgtgca cgacgagacc ctcgccgacc gctgcgcaga
     3601 tcgctgcacc gcgcgcctgg ccgagcagca acgtcgactg gggattcacg ttgacaaaca
     3661 ccttctcgat tgtcgcaaca tggggccgcg tctcgcggat cacgtcgccg agcgcgcgca
     3721 ggatggtgcc gaggcgttcg tgcaggtcac ccggcggcac gcgaatcacg cccgcagcca
     3781 ccggcccgag tcgcgacccg ctgacttcga tcaccccgaa ccccgtgcgg ttgaggccgg
     3841 ggtcgatgcc gaggcttcga agtgatgggg gcacgagggg attgtgccac gcagagtgtc
     3901 catgcttgcg ctatacgcac gcttcgcaac tagcaccttt gcctatccaa ctcgcgcttc
     3961 agggccgcgc ggcccgttac tttctgtgtc tgggcaaaga aagtaaccaa agaaagccgc
     4021 gcctgcgaag gcgcccctgc ggggtgcccg gtgctgctcg gggacgcggg cgggcctgca
     4081 aactcgcgcc ctgcggtcgc tcagacaggc aaggccctca aaggctcccg cgtcccctgc
     4141 gctgcgcggc gccttcggag gcattccaac gacgtcacct tgggcagcac cgtgcggctc
     4201 ccggcccaag cacagcgacg cggtgaatcc gctcggggac gccgcggcgg tgcggggcgg
     4261 cagcggggct gtgc
//
LOCUS       DATIRT010000040         5916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1086102, whole genome shotgun sequence.
ACCESSION   DATIRT010000040 DATIRT010000000
VERSION     DATIRT010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5916
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086102"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1389)
                     /locus_tag="VLN25_00850"
     CDS             complement(97..1389)
                     /locus_tag="VLN25_00850"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HSQ09155.1"
                     /translation="MRQQEVRSTGEAMRVAVLGAGVIGTSIAYYAARAGHEVTVVDRQ
                     SGPAQETSFANAGEVSPGYSAPWAGPGVPVKAIKWMLMTHSPLVVWPLLDPAMWRFGV
                     RMLANCTERAYRLNKSRMVPIAEYSRDCLKALRAETGISYDEREQGTLQLFRTARQLD
                     GIGKDVEILREYGVPFEVLDRAGFCRVEPALAVTQHKFVGALRLPGDETGDCFQFTHR
                     LAEMATGIGVEFRFDTPIESIAVAGGRIEAVHLAGGTLSADAYVCALGSYSTPVLLPI
                     GIHLPVYPVKGYSITVPIVDAGNAPESTIMDETHKVAVTRLGDRIRVGGTAELAGYSL
                     ALRVARRATLEHVVTDLFPRGGDVARATFWCGLRPMTPDGTPIIGPTRYPNLWLATGH
                     GTLGWTMAAGTGRVVADLLSGRAPEIDMTGLTLARYGH"
     gene            complement(1390..2997)
                     /locus_tag="VLN25_00855"
     CDS             complement(1390..2997)
                     /locus_tag="VLN25_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSQ09156.1"
                     /translation="MTLAGPEPAARPSPWAPLANPVFRLLWITWLTSNICMWMNDVAS
                     AWLMTTLTTSPVMVALVQSASTLPVFLLGLPSGALADILNRRRWFMFTQFWVATNAVL
                     LSVTMFAGWMSAPLLLALTFANGIGLAMRWPVYAAIIPELVTRRELPNAVALNGIAMN
                     TSRVIGPVIAGALIATLGTAYVYLLNAVLSLAAGFVIMRWRSEPKPSVLPGERFFGAM
                     RVGFQYVSQSPAMRSVLARIAVFFLQTTTVFALLPLVAKRMPGGSAWTYTVLLASLGV
                     GAVTVAFLLPRLRVLISRQRLLRDGTLLQAVAMVGLGFAPNVWTAVPAMMVAGGAWLA
                     VVNTLTVITQLSLPDWVRARGMSIYMMVMMGAASASAALWGQVASWIDVSWTYLAAAL
                     TGLAAGYLASRLYSDPENEVDLTPARILAEPVPAFPVEHQMGPVMVTVDYQIDPLRAE
                     GFAVVMRESRANRLQKGALSWGLFHDTNKPGHYIEYFLDDSWADHLRRFDRFTAADAE
                     LRERRYAFHIGAEPPVVSRYIAEPLGQ"
     gene            complement(3103..4773)
                     /locus_tag="VLN25_00860"
     CDS             complement(3103..4773)
                     /locus_tag="VLN25_00860"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HSQ09157.1"
                     /translation="MNATHEVTNQSTPFVGRNLYTNDPALQESVERLGGGWADAALRS
                     LGDRLGSQEVADWARLANAFPPQLRSFDRSGRRIDEVEFHPAWHEIMRLMVATGVHAE
                     PWVDPKPGAQVARAAKYLLFSQVENGAQCPVTMTYAVVPVMQRQAADVPGIARDWLPR
                     ILSNDYDPTSSAIGHKRGALLGMGMTEKQGGSDVRTNTTFAEADGRGEWGERYRLTGH
                     KWFFSAPQCDAHLVLAQTDRGSPAGLSCFLLPRWRADGTRNRIALQRLKDKVGNRSNA
                     SSEVEFDGAEAYLIGEIGRGIPTILEMGTHTRLDCAIGSAGILRALLTQALHHARERA
                     AFGRNLAEQPLMRNVLADLALESEAATLLSLRLARAFDGRHQRDEHEALIARVLTPAA
                     KYWICKRLPAAAAETMEIMGGNGYVEEGPFARFYREAPLNSIWEGSGNVMCLDVLRAL
                     GRVPAVREALIEELRSVRGATRTYDVYTAALVDDLARPDLAEAQARELTERIALAVQA
                     ALLLNGGCLDIAEAFCASRLASATWGRTFGTLPVGIDVTPILERALPA"
     gene            complement(4830..5657)
                     /locus_tag="VLN25_00865"
     CDS             complement(4830..5657)
                     /locus_tag="VLN25_00865"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSQ09158.1"
                     /translation="MPTLPGEIDGMDAQVARFPIAAFKTYTQFGPRDGAAGFFLDDDT
                     YGAPFIERARRLKVRNIAVHKGLAFGQRGYEFSTSRDIGPAAVRYPDMVFLIYHSGFD
                     TSVKEGPYDPQAKAGVDVLVRSMREAGRPKNVYAELGSTWRFVMRDPDQAAHLMGKLL
                     LTFGEDNILWGTDSIWYGSPQDQIQAFRAFEISREFQDKFGYPALTPEIKRKIFGLNA
                     VRVYGLDTNVLRQRLSKDRVKKARDDYLNDPQPSFATYGPRTRREFLALRELLGDMP"
     gene            complement(5730..>5916)
                     /locus_tag="VLN25_00870"
     CDS             complement(5730..>5916)
                     /locus_tag="VLN25_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09159.1"
                     /translation="WRKAAGPNAFKGMPNSNCGQADHVECFSSGALLKDVFMDSDTAM
                     GVLSHVPGGLDSNPLDF"
BASE COUNT         1047 a         1993 c         1912 g          964 t
ORIGIN      
        1 gagcaggtcg acggtggcgt cgatcgatgt gggtgcattc atgtccggac cttagccgat
       61 gtgcgattgc gtcgccagcg atgcagtatc tgcaattcag tgcccgtaac gcgccagagt
      121 caacccggtc atgtcgattt ccggagcgcg gccgctcagc aggtcggcga caacgcgccc
      181 ggtacccgcc gccatggtcc agcccagcgt gccatggccg gtggccagcc agaggttggg
      241 atagcgcgtc ggaccgatga tcggcgtgcc gtcaggcgtc atcggccgca gtccgcacca
      301 gaaggtggcg cgcgcaacgt cgccgccgcg cggaaaaaga tcggttacca cgtgctcgag
      361 cgtcgcccgg cgcgccacgc gcagtgccag gctgtagccc gccagttcgg cggtcccgcc
      421 gacacggatg cgatcaccga gtcgcgtgac cgccaccttg tgggtctcgt ccatgatggt
      481 cgactcgggc gcgttgccgg catcgacgat cggcacggtt atcgagtaac ccttcaccgg
      541 gtagacgggc agatgaatgc cgatcggcaa gagtaccggc gtcgaataac tgcccagtgc
      601 gcagacgtag gcgtcggccg acagcgttcc gccggcaaga tggactgcct cgatgcggcc
      661 gcctgcaacc gcgatcgact cgatgggcgt atcgaagcgg aactcgacgc cgataccggt
      721 ggccatctcg gcgaggcggt gggtgaactg gaaacagtcg ccggtttcgt cgccgggaag
      781 ccgaagagca ccgacgaact tgtgctgcgt cacggcgagc gcgggctcga cacggcagaa
      841 gcctgcgcgg tcgagcactt cgaacggcac accgtattcg cgcaggatct cgacatcctt
      901 gccgatgccg tcgagctgcc gggccgtgcg gaagagctgc agcgtgccct gttcgcgctc
      961 gtcataggag atgccggtct cggcccgcag tgccttcagg caatcgcggc tgtactcggc
     1021 gatcggcacc atgcggctct tgttcagccg gtaggcacgt tcggtgcaat tggccagcat
     1081 gcgcaccccg aaacgccaca tcgccgggtc gagcagcggc cagacgacga gcgggctgtg
     1141 cgtcatcagc atccacttga tcgccttgac cggtacgccg ggaccggccc agggcgccga
     1201 gtagcccggc gagacttcgc cagcattggc aaagctggtt tcctgcgccg gtccggactg
     1261 gcggtcgacg accgtcacct cgtgacccgc cctggccgcg taatacgcga tcgacgtacc
     1321 gattacaccg gctccgagca ctgcgacacg catcgcttct cctgtggacc gtacttcttg
     1381 ctgtcgaatc tattgtccga ggggttcagc gatgtagcgt gagaccactg gcggttcagc
     1441 gccgatgtgg aaggcgtaac gccgttcgcg cagttcggcg tcggccgccg tgaaccggtc
     1501 gaagcgtcgc aagtggtcgg cccaggaatc atcgaggaaa tactcgatgt aatgccctgg
     1561 cttgttcgtg tcgtgaaaca gtccccacga cagcgcgccc ttctgcagtc ggttcgcgcg
     1621 gctctcgcgc atcacgacgg cgaacccctc ggcgcgcagc gggtcgatct ggtaatcgac
     1681 cgtgaccatg accggtccca tctgatgctc gaccggaaag gccggcaccg gttcggccag
     1741 gatgcgagcc ggagtcaggt cgacctcgtt ctcaggatcg gagtacagac ggctcgccag
     1801 atatccggcg gcgagacccg tcagcgctgc tgccaggtaa gtccaggaga cgtcaatcca
     1861 gcttgcgacc tggccccaca gtgccgcgct cgccgacgcg gcgcccatca tcaccatcat
     1921 gtagatcgac ataccgcgcg cgcgcaccca gtcgggcagc gacaactgcg tgatgaccgt
     1981 cagcgtgttg acgaccgcca gccacgcgcc gccagccacc atcatggcgg gtacggctgt
     2041 ccacacattc ggcgcgaacc caagaccgac catcgcgaca gcctgcagca gcgtcccgtc
     2101 gcgcagcagc cgctgacgcg agatcagcac acgcaatcgc ggcagcagga acgccacggt
     2161 gaccgcgccg acacccagcg acgccagcag caccgtatag gtccaggcac tgccgccggg
     2221 catgcgcttg gccaccagcg gcagcagagc gaacacggta gttgtctgca ggaagaacac
     2281 cgcaatgcgc gccagcaccg agcgcatcgc gggcgattgt gagacgtact ggaaaccgac
     2341 gcgcatcgcg ccgaagaagc gctctcccgg cagcacgctc ggcttgggct ccgagcgcca
     2401 acgcatgatc acgaacccgg ccgcgagcga gagcaccgcg ttgagcagat agacgtaggc
     2461 cgtacccagg gtcgcgatca gcgctccggc aatcaccggg ccgatcacgc gcgacgtgtt
     2521 catcgcgatg ccgttcaggg ccacggcatt cggcagttcg cggcgtgtca cgagctccgg
     2581 aataatcgcc gcgtacactg gccagcgcat cgccaggccg atgccattgg cgaaagtcag
     2641 cgccagcaac agcggcgcac tcatccatcc ggcgaacatc gtcacgctga gcagcacggc
     2701 gttggtcgcc acccagaact gcgtgaacat gaaccagcgc cggcgattga ggatgtctgc
     2761 gagcgccccg ctcggcagtc cgagcaggaa caccggcagc gtggaagccg actgcaccag
     2821 tgcgaccatg accggagacg tcgtcagggt ggtcatcagc cacgctgacg cgacatcgtt
     2881 catccacatg cagatgttcg atgtaagcca ggtgatccac agcagccgga agaccggatt
     2941 ggcgagcggc gcccacggcg acggccttgc cgcgggttcg ggtccggcga gcgtcatgca
     3001 agcattgtat tgacgtcgcc gctggcgctg cgatggccgt cgccgcggca gtccccacgg
     3061 gcgcctccaa accgactccc cttaggcggg cttggccacg cattacgccg gcagcgcacg
     3121 ctcaaggatt ggcgtgacgt cgataccgac gggcagtgtg ccgaatgtgc gcccccatgt
     3181 cgccgacgcc aggcgcgacg cgcagaaagc ctcggcaatg tccaaacaac cgccgttgag
     3241 gagcagcgcg gcctgaacgg cgagcgcaat gcgctcggtc agttcgcgtg cctgcgcttc
     3301 tgctaggtcg gggcgcgcaa ggtcatcgac aagcgccgct gtatagacat cataggttcg
     3361 agtggctcct cgcactgaac gcaactcctc gatcagtgcc tcgcgcaccg cgggcacccg
     3421 gcccagcgcg cgcagcacgt ccaggcacat gacgttgccc gagccctccc agatcgagtt
     3481 cagcggcgct tcgcggtaaa agcgggcgaa cggaccctcc tcgacgtagc cgttgcctcc
     3541 catgatctcc atcgtctccg ctgcggcggc gggcagccgc ttgcagatcc agtacttcgc
     3601 cgccggtgtc agcacgcgcg cgatcagcgc ctcgtgctca tcgcgctggt ggcggccgtc
     3661 gaaggcgcgc gccagccgca acgacaggag cgtggccgcc tcggattcga gcgccaggtc
     3721 ggcgagcacg ttgcgcatca gcggttgctc ggccaggttg cgaccgaacg ccgcccgttc
     3781 ccgagcgtga tgcagcgcct gcgtgagcag cgcgcgcagg atgccggcgg atccgatcgc
     3841 gcaatccagc cgcgtgtgcg ttcccatttc gagaatggtg ggtatgccgc gaccgatctc
     3901 gccgatcagg taagcctcgg cgccgtcgaa ctcgacctcg ctggacgcat tcgaacgatt
     3961 gccgaccttg tccttgagcc gttgcaaggc gatgcgattg cgcgtcccgt cggcgcgcca
     4021 acgcggcaga agaaagcacg acagcccggc cggcgacccg cggtcagtct gcgcaagcac
     4081 caggtgtgca tcgcactgcg gcgcggagaa gaaccatttg tgccccgtca ggcgatagcg
     4141 ttcgccccac tcgccacgtc cgtcagcttc tgcgaatgtg gtgttggtgc gaacgtcgga
     4201 gccgccctgc ttttccgtca tgcccatgcc gagcagcgcc ccgcgcttgt gaccgatcgc
     4261 gctcgaggtc gggtcgtagt cgttcgacag gatgcgcggc agccagtcgc gcgcgatgcc
     4321 cggcacgtcg gcggcctgcc gctgcatgac cggcacgacc gcataggtca tcgtgaccgg
     4381 acactgagcg ccgttttcca cctgcgaaaa cagaaggtac ttcgccgcgc gcgcaacctg
     4441 tgcgcccggc ttcggatcga cccacggttc ggcatgtaca ccggtggcga ccatcagccg
     4501 catgatctcg tgccatgccg ggtgaaactc gacttcgtcg atgcgccggc cgctgcgatc
     4561 gaaactgcgg agctgcggcg gaaacgcgtt tgcgagccgc gcccagtccg cgacctcctg
     4621 gctgcccagc cgatcgccga gtgaccgcag ggcggcatcg gcccagccgc caccaagccg
     4681 ctcaacactc tcctgcagcg ccggatcgtt cgtgtagagg ttgcggccga caaacggggt
     4741 cgactggttg gtgacttcgt gggttgcgtt catctcagtc tcgcgccagg tgcaccggcg
     4801 aacggcgcga actcatcgtc cggcgtgccc tacggcatgt ctccgagcag ctcgcgcagc
     4861 gccaggaatt cgcgtcgtgt gcgcggcccg taggtcgcaa agctcggctg cggatcgttc
     4921 aggtaatcgt ccctcgcctt cttcacgcgg tccttcgaca ggcgctgccg caggacgttg
     4981 gtgtcgaggc cgtacacgcg caccgcattg agtccgaaaa tcttgcgctt gatttctggt
     5041 gtcagcgcgg ggtagccgaa tttgtcctgg aactcgcggc tgatctcgaa ggcgcggaac
     5101 gcctgaatct ggtcctgcgg actgccgtac cagatcgagt cggtgcccca caggatgttg
     5161 tcctctccga aggtcagcaa cagcttgccc atcagatggg cggcctggtc gggatcgcgc
     5221 atgacgaagc gccaggtgct gcccagctcc gcatacacgt tcttcggacg gcccgcctcg
     5281 cgcatcgaac gcaccagcac atcgacgcct gccttcgcct gcgggtcgta gggaccttcc
     5341 ttgacactcg tgtcgaagcc cgagtgatag ataaggaaca ccatgtccgg gtagcgcact
     5401 gccgccggcc cgatgtcgcg cgaggtcgag aactcgtagc cacgctgccc gaacgcaagg
     5461 cccttgtgca cagcgatgtt gcgcaccttg agcctgcgtg cgcgctcgat gaaaggcgcg
     5521 ccgtacgtgt cgtcgtcgag gaaaaatccc gccgccccgt cgcgggggcc gaactgcgtg
     5581 taggtcttga acgcggcaat cggaaagcgc gcaacctgtg cgtccatgcc gtcgatctcg
     5641 ccgggcaggg tcggcatgca acggccgtgc agcagcaggc gctcagtgcc gtcgagcgtt
     5701 tttgccgcac accgtgtcgc cccggccgcc tagaagtcaa gtggattgga atcgagaccg
     5761 cccggtacat gcgacagcac gcccatcgcg gtatcgctgt ccatgaacac gtccttcagc
     5821 agcgcaccgc tggagaagca ctcgacgtga tcggcctggc cgcagttgct gttcggcatg
     5881 cccttgaatg cgttcgggcc ggcggccttg cgccac
//
LOCUS       DATIRT010000041        11916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_791218, whole genome shotgun sequence.
ACCESSION   DATIRT010000041 DATIRT010000000
VERSION     DATIRT010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11916
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791218"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(305..1783)
                     /gene="mgtE"
                     /locus_tag="VLN25_00875"
                     /pseudo
     CDS             complement(305..1783)
                     /gene="mgtE"
                     /locus_tag="VLN25_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015836.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
     gene            complement(1954..2658)
                     /gene="mtgA"
                     /locus_tag="VLN25_00880"
     CDS             complement(1954..2658)
                     /gene="mtgA"
                     /locus_tag="VLN25_00880"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HSQ09160.1"
                     /translation="MKFRGSFARWIIRIAVLVVLALVAIQLWYAGWIAWWRWNDPDET
                     AFMRRERAALQVRNPKAELKHRWVPYERIATSLKRAVITAEDARFAEHEGVDWEAIEK
                     AYKDNVKRGRPARGGSTITQQLAKNLFLSPDRSYVRKAQELVITYMIEAFWDKRRILE
                     VYLNVVEWGEGVFGAEAATRYYFGVGAAQLSSEQAARLAAFLPNPKRYGRVRSGPYLD
                     GRTGAILRYMPDAIVP"
     gene            complement(2713..3561)
                     /gene="aroE"
                     /locus_tag="VLN25_00885"
     CDS             complement(2713..3561)
                     /gene="aroE"
                     /locus_tag="VLN25_00885"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HSQ09161.1"
                     /translation="MSAPDKYAVVGNPIAHSRSPEIHERFARQTRQRMTYERIEVPLE
                     GFEDRALALRDAGYRGLNVTVPFKLDAAKLSDDLMPRARLAGAVNTLVFDGDTIIGDN
                     TDGIGFVRDVRERLKFDLSDCSVLVLGAGGAVRGVIGSLLDASPRWIAVANRTHQRAE
                     ELGEEFGVEAIHLDEIPAEHFDLIVNGTTTGLKHDAPPIDPETFNDCTLAYDLVYAAD
                     PTPFMELARSGGAKTVCDGLGMLIEQAAESFLLWRGVRPDTAAVYRELRELLSQAAAE
                     NATVAR"
     gene            complement(3558..4469)
                     /locus_tag="VLN25_00890"
     CDS             complement(3558..4469)
                     /locus_tag="VLN25_00890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HSQ09162.1"
                     /translation="MDTAAERMNLADESPRGLAAFLADRVLVMALTVSIGLHMTVLAV
                     RFVDPDFLRVRSTDPPLEIILVNARSETRPGKAEALAQANLDGGGSNEAGRRASPLPN
                     AQQLQDGDALDSARRLVEQLEEEQKKLLTALRDSLQMRPLPRKESAAESVQPGSADEE
                     TREKLARAQAEIAKQVSDYQKRPRKHHFMPSTSEYRYARYVEDWRARVERIGNENYPD
                     DARGKVYGVLRMTVAVRKDGSVIEAAVDRSSGSPVLDRAARRIVNMAAPFPPFPPEIA
                     RDTDVLEITRTWIFTNDQLSTRSSVTQ"
     gene            complement(4541..6565)
                     /locus_tag="VLN25_00895"
     CDS             complement(4541..6565)
                     /locus_tag="VLN25_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HSQ09163.1"
                     /translation="MNLLFEEDGTFRAGSVLSSTDASFQVELPSGKRAKVKASHVLLQ
                     FDDLPAAKLLERAHAESESIDIDFLWEAAPQDDFGFRDLARDYYGAKPTSVQEAALLI
                     RLHSAPVYFYRKGRGRYRPAPPDTLKAALAAVDRKRRQDELRQQYVDALKQGNAPAAI
                     AAQAVSMLVHPDRGSLEFKALEQAAVETQTTPLRLLLATRAIESPYRWHVDSFLAQHF
                     PRGAGFPANLPTPELAGDLPLADVMAFSIDDSATTEIDDAFSVTPSGDGRVRVGIHIA
                     APAVAFEREHALDAVARARMSTVYAPGLKITMLPPEWVAAYSLVEGREVPVLSLYADV
                     DRSTFDVLGTESKVERVRIVSNLRHDRLDDIVTDESIADASFAAPFANELSFLWHFAR
                     SLLARREAVRGRPEPLGRVDYSFEIDGEGEHAHVSIKPRRRGAPLDLLVAELMILANS
                     TWGRWLADRAVAGIYRSQSMGRVRMSTTPAAHEGIGVSHYAWSTSPLRRYVDLVNQHQ
                     LIACVRNKAAPYAAGDADLFAIVSGFDAAYGAYAEFQERMERYWGLRWLRQENVQRIG
                     ATVLRGDVLRLSGLPFVTRLPGLPELPRGQQLELDVIGGDPVDLTFEARLHRVLAEQS
                     GEDVDENDNAAQDSEVQDGQVQVGDGGGVAQVPSFPKTPGATLPPQGLID"
     gene            complement(6573..7172)
                     /locus_tag="VLN25_00900"
     CDS             complement(6573..7172)
                     /locus_tag="VLN25_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HSQ09164.1"
                     /translation="MNDTFLSAVLLLLLVADPFGNIPIFSSALRDVPQQRRLFIVLRE
                     CLIAFFVLTLFVFAGRPFLETLGLSETSLQIGGAVVLMLVALRMVFPSPDGVYGASPS
                     GEPFIVPLAVPALAGPSALATVMLLVSKNPERWPEWVAAIAVVMAISALILVSAARLQ
                     ELLGERVTLAFERLMGLVLAAIAVELLLRGVRAFVATLP"
     gene            complement(7169..7657)
                     /locus_tag="VLN25_00905"
     CDS             complement(7169..7657)
                     /locus_tag="VLN25_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HSQ09165.1"
                     /translation="MTPSTPLPSSAERVQRALDTAGVGARIVELDVAARTAQQAADAL
                     GIEVGQIAKSLIFRAATSRRAVLVIAAGDRRVSEPRIAQLIGEPIERAPPDFVREHSG
                     FAIGGVAPIAHAQPMLTYIDASLRRFETVWAAGGTPHCVFPITPEMLVRISAGNETQI
                     DA"
     gene            complement(7661..8401)
                     /gene="fabG"
                     /locus_tag="VLN25_00910"
     CDS             complement(7661..8401)
                     /gene="fabG"
                     /locus_tag="VLN25_00910"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HSQ09166.1"
                     /translation="MKRLLGKVCIVTGAAQGIGLATARRFLEEGATVALCDIRRESVD
                     AAVEALQDLGSVEGHVVDVTKRDQIDAMVEAVRMRHHAIDVLVNNAGITLDARLQKMD
                     EDRFDRVIEVNLKGTFNCARAVVDTMVEQGRGVILNASSVVGIYGNFGQTNYAASKFG
                     VIGFVKTWSRELGPKGIRCNAVAPGFIETPILRTIPDKVLHQMKERVPLRRLGTPEDV
                     ANVYAFLASDDASYINGAVIEVAGGLTV"
     gene            complement(8398..9003)
                     /locus_tag="VLN25_00915"
     CDS             complement(8398..9003)
                     /locus_tag="VLN25_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HSQ09167.1"
                     /translation="MIVYLHGFRSSPASRKATMLREAMDRRGRSADYVCPALPASPAR
                     AIEVAQSAVAGIAPARVALIGSSLGGYYATWMAERLGCRAVLLNPAIRPQDDLARHLG
                     VQPVYFSDAQIEFRREYLTELEAIDTPTITRPNRYFLVAATGDVVIDYRSMVRKYEGA
                     RQHVIAGSDHELSDFSQYLDEVLAFCDAGNTTSISEGREVQ"
     gene            complement(9000..10349)
                     /gene="mpl"
                     /locus_tag="VLN25_00920"
     CDS             complement(9000..10349)
                     /gene="mpl"
                     /locus_tag="VLN25_00920"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HSQ09168.1"
                     /translation="MHIHILGICGTFMGGLAQLALAAGHRVSGCDASVYPPMSDQLAA
                     AGVRLIEGYGAEQIGIAPDLWVIGNVVTRGNPLMEEILERGLPFTSGPQWLAENVLAG
                     RHVLGVAGTHGKTTTTAMLTCILDHAQMDPGFLVGGVPTDFGVSARLTASRFFVIEAD
                     EYDTAFFDKRSKFIHYRPRTTILNNLEFDHADIFADLAAIETQFHHLVRTLPATARLI
                     VNERNMALERVLARGCWSEVERFGGERWHVGPGAPHEVVHDGRIVGSLRLVLPGEHNR
                     MNALAAIVAAHHVGVGPADALEALASFGGVKRRLEVRGTVAGVTVIDDFAHHPTAIRE
                     TIDALREEVGDARIIAVLEPRSNTMKQGAVKAELAPSLQGADRVFCYAGSVGWNVVEA
                     LAPLGERASIHAELQALVDDVVACARAGDRVVVMSNGGFGGIHERLLDRLASRAAEA"
     gene            10460..11119
                     /locus_tag="VLN25_00925"
     CDS             10460..11119
                     /locus_tag="VLN25_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09169.1"
                     /translation="MTSSNESLRTELAAAAARLIAEEGCDYGQAKRRALHDLLGDTGS
                     RRLLPDNAEIEHELRRHLQLFAADTHPALLAALRRSAAGVMARLADFNPHLVGAVLSG
                     TATEHSDIELHLFTDSAKDVEVVLMESRIEFEAEAADDHDRPLPLERLTFVLPAREPG
                     LPPSLRLIGVRLHVYDRDAIRVAPRHRALATSEFDVHPIAAGGRANLAALRQLIEDSE
                     P"
     gene            11116..11652
                     /locus_tag="VLN25_00930"
     CDS             11116..11652
                     /locus_tag="VLN25_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HSQ09170.1"
                     /translation="MNASRRRLLFAGVGAIALAGGAGLAVYRLRPDPVGAAAVATLFA
                     QSFDDAQGQPQPMVQWKGQWLVLNFWATWCTPCVEEMPTLQQVARDYGSRGVAVVGLG
                     IDSPAAIRRFRSELKLELPLLVAGAGGTELARELGNPSGALPYTVLISPKGAVEQAHL
                     GLLRPELLRSWLDARLSS"
     gene            11764..>11916
                     /locus_tag="VLN25_00935"
     CDS             11764..>11916
                     /locus_tag="VLN25_00935"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006756005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HSQ09171.1"
                     /translation="MSSRILVLHGPNLNLLGTREPAIYGGRTLAQIESELSDVARRYA
                     ADLVFFQ"
BASE COUNT         2117 a         4059 c         3720 g         2020 t
ORIGIN      
        1 agcggaactg atcatttagt cgattgcgct ccggagcggc cgcgcgctcc gcctagtaac
       61 aaaaacacgg cacgcacctt ggacctctcg ccgagcgccc tcctcgccag gcgcggaggg
      121 aaatttaaac ccggttcccc ggcaagcacc ccgggctggc gtgcctgctt ttccctttcg
      181 gcggagcgcg cggccgctcc ggagcgcagc cgactaacta gacttaagcg gctcgcgcaa
      241 cgcgcgagtc gtaccggagc aggtgcacgt gcatcggcgg agccgatgcg tacacctgcg
      301 acgcctaccg aaagaagagc gttgccaacc cgagaaaaat gaagaaccca ccggagtcgg
      361 tggtgaaggt caacagcacg gaagcgccga tcgccggatc gcgaccggaa gcccgtaggg
      421 ccatcggcac ggcgagtccg acggctgcag cgaccagcat gttcagaatc atcgccagga
      481 acatgaccag tcccagcagt acgccgttgg gtgtgtcgcg atacagcagc catgccatca
      541 gtccggccag gccaccccac accgcgccgt tcagcacggc gatgccgatc tccttcaata
      601 tcagccggcg ctggttacct gacgtgacct ggccgagcgc gagcgaacgc accagcaaag
      661 tcagggtctg gttgcccgag ttgccggcga cgccggcgac gatcggcatc agcgcggcca
      721 gtgcgacgac tttctcgatc gtgccttcaa aggcgtcaat gacacgactg gcgaaaaatg
      781 ccgtgcacag gttgaccgcc agccacagcc agcggttctt cgccgaattc cagaccgacg
      841 cgaagatgtc ctcgtcttcg cgcaggccgg cctgcgccag cgcggcttcc tcgccctcct
      901 cgcggatgac gtcgacgacc tcgtcgacag tcagccgccc gaccagccgc ccgccggaat
      961 cgacgaccgg cgcggacacg aggtcctagc gctcgaaggc ctgtgccgcc tcgccaccgt
     1021 cgtccagcgg tgacagggtc agcaggtcgc tcttcatcac cgcggtgacc tcggtgtcag
     1081 gttcattgat cagcagccgg tcgagcggca gcgcaccctt aagcacttcg tgccggtcga
     1141 cgacgaacac ctgatcggtg tgatccggca gctcgtcgaa gcgccgcaga tagcgcagca
     1201 cgacctcgag cgtgacatcc tcgcggatcg tgatcatctc gaaatccatc agcgcgccga
     1261 ccgacccctc cgggtaggac atcgccgcgc gcagctgcgc ccgctcctcg ctggtcagtc
     1321 cctcacggac ctcctcgacg acgtccgccg gcaggtcgtg cgcaatggcc gcgatttcgt
     1381 cggtctcgag actctcgacc gcatccacga gttcctcgcg gtccatcgag gcgatcagcg
     1441 tctcgcggac tgcgtcgttc acctcgatca ggatctcgcc gtcacggtcc gccttgacgc
     1501 agtcccagac gaccagtcgg tcgtccatcg gcagcgcttc aagcaggtag gcgatgtcgg
     1561 cggcgtgcag ccggtcgagt atcgagcgca gctcgttcag gtgctggcga tgcaccagtt
     1621 gctcgaccag cgccttcttc tgctccgcag gcgtctgctc gcgatgcacc agcgcctcga
     1681 cgcggcgctg cttctcgagc acctgctgga cgcgcacgag cgcgcgctgc gcggcctcgg
     1741 ggtcgcgcac cggagcatcg cgttgcgcgg cgcggtcctg cagttcctgt tcggtcacgg
     1801 tcgctccctc gcgctgcgcg ccgccccttc gcgagggacg ctcgccttcc ggtgaccgca
     1861 aggcgagcac agcgcgcctc taaaacgagg cggttcaaag gctgcgggat tgtaagcgcg
     1921 cccttgcgca ggcagccgtc gagacgagac gtgttacgga acgatggcgt cgggcatgta
     1981 gcgcaggatc gctccggtgc ggccgtcgag atacgggccg gaacggacac gaccatagcg
     2041 cttcggattg ggcagaaatg cagccagccg ggcagcctgc tccgagctca actgcgccgc
     2101 gcccacgcca aagtaatagc gcgtggctgc ctctgcgcca aacacacctt caccccactc
     2161 gacgacgttc aggtagacct cgaggatgcg gcgcttgtcc cagaacgcct cgatcatgta
     2221 cgtaatcacg agttcctgcg ccttgcgcac gtaactgcgg tccggagaaa ggaacaggtt
     2281 cttcgccagc tgctgcgtga tcgtcgaacc gccgcgggcc ggacgaccgc gcttgacgtt
     2341 gtccttatag gccttctcga tcgcctccca gtccaccccc tcgtgttcgg cgaagcgcgc
     2401 atcttcggcc gtgatcaccg cccgcttgag actcgtcgcg atgcgctcgt acggcaccca
     2461 ccgatgcttc agttcggcct tcggattgcg gacctgcagt gccgcacgtt cgcgccgcat
     2521 gaacgcggtt tcgtccggat cgttccagcg ccaccaggcg atccatcccg cgtaccagag
     2581 ctggatagcc acaagcgcca gcaccaccag cactgcaatg cggatgatcc agcgcgcgaa
     2641 tgaacctcgg aacttcacag aggggttcga aacgtcgcga caccggcgcc accacacacc
     2701 ggcgagcgcg tcctagcgcg caacagtggc gttctcggcc gcggcctggc tcagcagttc
     2761 gcgcagttcg cggtacacgg cggcggtatc gggccgcacc ccgcgccaca gcaggaagga
     2821 ctcggctgcc tgctcgatca gcatgcccag tccgtcgcag accgtcttgg caccgccgct
     2881 gcgggccagt tccatgaagg gtgtcggatc ggccgcgtag acaaggtcat aggccagcgt
     2941 gcagtcgttg aatgtctccg gatcgatcgg cggagcgtcg tgcttcagcc ctgtggtcgt
     3001 gccgttgacg atgaggtcga agtgctcggc cgggatctcg tcgagatgga tcgcctcgac
     3061 gccgaactcc tcgcccagtt cctcggcccg ttggtgcgtg cggttggcca ccgcaatcca
     3121 gcggggactg gcgtcgagca gcgatccgat gacgccgcgc acggcacccc cggctccgag
     3181 aaccagcacc gagcagtcgc tcaggtcgaa cttgagccgc tcccgcacat cgcgcacaaa
     3241 tccgatgccg tccgtgttgt cgccgatgat cgtgtcccca tcgaacacga gcgtgttgac
     3301 cgcgccggcg aggcgggccc gcggcatcag gtcatcggac agtttcgccg catcgagctt
     3361 gaacggcacc gttacgttga ggccgcgata gccggcgtcg cgcagcgcaa gcgcacggtc
     3421 ctcgaatccc tcgagcggca cctcgatgcg ttcgtacgtc atgcgctgtc gcgtctgccg
     3481 ggcaaatcgc tcatggatct caggcgaccg actgtgggca atcggattgc cgacgactgc
     3541 gtacttgtct ggcgcgctca ttgagtgacg ctgcttcgtg tcgacaactg gtcgttggtg
     3601 aaaatccagg ttcgcgtgat ctcgagtacg tcggtgtcgc gggcgatctc cggagggaac
     3661 ggaggaaacg gagcagccat gttaacgatg cggcgggcgg cacggtcaag tacgggcgat
     3721 cccgacgatc ggtcgactgc ggcctcgatc accgatccgt ccttgcgcac cgcgacggtc
     3781 atgcgtagca caccgtacac cttgccgcgc gcatcgtccg gataattttc attgccaatc
     3841 cgctccacgc gtgcgcgcca gtcttcgaca tagcgagcgt agcgatattc cgaggtgctg
     3901 ggcatgaaat gatgcttgcg cggacgcttc tggtagtccg acacctgctt ggcgatctcg
     3961 gcctgcgcac gcgcgagctt ctcgcgcgtt tcctcgtccg ccgatccggg ctgcacggat
     4021 tccgccgccg attctttcct gggcagcggg cgcatctgca gcgaatccct gagcgcggtc
     4081 agcaacttct tctgctcttc ctcgagttgc tcgacaaggc ggcgcgcaga atcgagcgcg
     4141 tccccgtcct gcagctgctg cgcgttcggc agcggtgacg cgcgccgccc cgcctcgttc
     4201 gagccacccc cgtcgagatt ggcctgcgcc agcgcctcgg ccttgcccgg acgcgtctcg
     4261 ctccgggcat tgaccaggat gatctcgagc ggaggatcgg tcgagcgcac gcgcaggaag
     4321 tccgggtcga cgaagcgcac ggcaagcacc gtcatgtgca ggccgatgga caccgtcaat
     4381 gccatgacga ggaccctgtc ggccaggaag gccgcaaggc cccgcggtga ctcgtcggca
     4441 agattcatgc gctcggcagc agtgtccatg gaggcgaaac gattgtagcc atggcctgcg
     4501 cgcgtcgatc acacgatcac gcgactgtcg tgcggatgac tcaatcgatc agaccctgcg
     4561 gcggcaaggt cgcgccaggg gtcttcggga acgatggcac ctgggccacg ccccccccgt
     4621 cacctacctg cacttggcca tcctgcactt cgctgtcctg cgcagcgttg tcgttctcgt
     4681 caacgtcttc gcctgactgc tccgcgagta cgcgatgaag acgtgcttcg aacgtcaggt
     4741 cgaccgggtc gccgccgatg acatccagtt cgagctgctg gccgcgcggc agctcaggaa
     4801 ggcccggcag acgcgtcacg aacggcagcc cggaaaggcg cagcacgtca ccgcgtaaca
     4861 ccgtggcgcc gatgcgctgc acgttttcct ggcgcagcca gcgcagcccc caatagcgct
     4921 ccattcgctc ctggaactcg gcataggccc cgtacgcggc gtcgaagccc gacacgatcg
     4981 cgaacagatc cgcgtcacct gcagcgtagg gcgccgcttt gttgcgcaca caggcgatca
     5041 attggtgctg attgaccaag tcgacgtatc gcctcagcgg cgatgtgctc caggcgtagt
     5101 gactgacacc gataccttcg tgtgcggccg gcgtggttga catgcgcacg cgccccatcg
     5161 attgcgagcg atagatcccc gcgaccgcac ggtccgcgag ccagcgaccc cacgtgctgt
     5221 tggcgaggat catcagctcg gctacgagca ggtccagcgg ggccccccgc cgtcttggct
     5281 tgatgctcac atgcgcgtgc tcgccttcgc catcgatctc gaacgagtag tcgacccgcc
     5341 cgagtggttc cggccgtccg cgcactgcct cgcgccgcgc cagcagcgag cgggcgaagt
     5401 gccacaggaa cgaaagctcg ttcgcgaacg gcgccgcgaa gctcgcgtcg gcgatggact
     5461 catcggtgac gatgtcgtcg agccggtcgt ggcgcaggtt ggagacgatc cgcacgcgct
     5521 cgaccttcga ttcggtcccg agcacgtcga aagtgctgcg gtcgacgtcg gcgtatagcg
     5581 acagcaccgg aacttcacgc ccctccacca gcgagtacgc cgcgacccat tcgggcggca
     5641 gcatcgtgat cttcagaccg ggcgcataga ccgtcgacat gcgcgcccgc gcgaccgcat
     5701 ccagcgcatg ctcgcgttcg aaggcgaccg ccggcgccgc tatgtggatg ccgacgcgca
     5761 cccgaccgtc gcccgacggt gtcaccgaga aagcatcgtc gatctcggtc gtggcgctgt
     5821 cgtcgatcga gaatgccatc acatccgcga gcggaagatc gccagccagc tctggcgtag
     5881 gcaggttcgc aggaaatccc gcaccccgag gaaagtgctg cgcgagaaag ctgtcgacgt
     5941 gccagcgata cggcgattcg atcgccctgg tggccagcaa caggcgcagt ggcgtcgtct
     6001 gcgtttcgac tgcggcctgt tccagtgcct tgaactccag cgacccccga tccgggtgca
     6061 cgagcatgga cactgcctgg gcggcgatgg ctgcgggcgc gttgccttgt ttcaacgcat
     6121 cgacatactg ctggcgcagt tcgtcctggc ggcgtttgcg gtcgaccgcg gcaagcgcgg
     6181 ccttgagtgt gtcagggggt gccggccggt accgtccgcg acccttgcgg tagaagtaga
     6241 ccggcgccga atgcaggcgg atcagcagtg ccgcttcctg cacgctcgtc ggcttggcgc
     6301 cgtagtaatc ccgcgccaga tcacggaagc cgaagtcatc ctgcggcgcc gcctcccaga
     6361 ggaaatcaat atcgatcgat tcggactcgg catgagcgcg ctcgagcagc ttcgccgccg
     6421 gcagatcatc gaactgcagc agcacatgcg aggctttgac cttggcgcgc ttgccgctcg
     6481 gcagttcgac ctggaacgac gcatcggttg aggacaggac acttccggcc cggaacgtcc
     6541 cgtcttcctc gaaaagaaga ttcaccgtag cctcatggca gcgttgccac gaacgcccgc
     6601 actccgcgca gcagcagttc aaccgcaatc gccgccagca ccagccccat cagacgctcg
     6661 aatgcgagcg taacccgctc gccaaggagc tcctgcagcc gtgctgccga gaccaggatc
     6721 aatgcactga tggccatgac cacggcgatc gctgccaccc actccggcca gcgctccggg
     6781 ttcttcgaga ccagcagcat gaccgtcgcc agcgccgacg gacccgccag cgcaggaacc
     6841 gccaggggca cgatgaacgg ttcgccgctt ggcgacgcgc catacacgcc atcgggcgac
     6901 ggaaacacca tgcgcaaggc aaccagcatc agcacgacgg cgccgccgat ctgcagcgag
     6961 gtttcggata ggccgagggt ttcgagaaat gggcggcctg caaacacgaa gagtgtcagc
     7021 acgaagaacg cgataaggca ctcccgcagc acgatgaaaa ggcgccgctg ctgcgggaca
     7081 tcgcgcaggg cggaactgaa gatcggaatg ttgccgaacg gatcggcgac cagcaggagc
     7141 agcaagaccg ccgaaagaaa ggtgtcgttc atgcgtcgat ctgcgtttcg ttgccggcac
     7201 tgatacgcac cagcatctcg ggagtgatcg gaaagacgca atggggcgtt ccccccgccg
     7261 cccacacggt ttcaaagcgc ctcaacgagg cgtcgatgta ggtcagcatc ggctgcgcat
     7321 gcgcaatcgg tgcgaccccg ccgatcgcga accccgagtg ttcgcggaca aagtccggcg
     7381 gggcccgttc gatcggttcg ccgatgagct gcgcgatccg cggttcactc acgcgccgat
     7441 cacctgccgc gatcacgagc acggcacgcc tcgacgtcgc agcccggaag atcagcgact
     7501 tcgcgatctg cccgacctcg atcccgagtg catccgcggc ctgctgtgca gtgcgggccg
     7561 ccacatcgag ttcgacaatg cgcgcaccaa cgcccgcggt gtcgagcgcg cgctgcaccc
     7621 gctctgcgga agacggcaac ggcgtggagg gcgtcatgaa ctaaacggtc aggccgcccg
     7681 ccacttcgat caccgcgcca ttgatgtatg acgcatcatc ggaggccagg aacgcataga
     7741 cattggcgac gtcctcgggg gtaccaaggc gccgcagcgg cacgcgttct ttcatttgat
     7801 gcagcacctt gtccggaatc gtcctcagga tcggggtctc gatgaatccg ggtgccactg
     7861 cattgcatcg aatgcctttg ggtcccagtt cgcgcgacca ggttttgacg aaaccgatca
     7921 cgccgaactt gctggccgca tagttggtct ggccgaagtt gccgtagatc ccgaccacgc
     7981 tggaggcatt gagtatgacg ccacggccct gctcgaccat cgtgtcgaca accgcgcgcg
     8041 cacagttgaa tgtgcccttg aggttgactt cgatcacccg atcgaaccgg tcctcgtcca
     8101 ttttctgcag gcgcgcgtcg agcgtgatgc cggcattgtt gaccagcaca tcgatggcgt
     8161 gatggcgcat gcggacggct tcgaccatgg catcgatctg gtcgcgcttg gtcacgtcga
     8221 cgacatggcc ctcgacactg ccgagatcct gcagcgcttc caccgccgca tcgacgcttt
     8281 cgcggcggat gtcgcacagc gcgactgtcg ccccttcctc gagaaagcga cgcgcggtcg
     8341 ccagaccgat gccttgcgcg gcaccggtca cgatgcacac tttgcccagc aaacgcttca
     8401 ttgcacttcc cttccctctg aaatcgatgt cgtgtttccc gcgtcgcaga acgccagcac
     8461 ctcgtcgagg tactgggaaa agtcggacag ctcgtgatca ctgcccgcga tcacgtgctg
     8521 ccgcgcgccc tcgtacttgc gcaccatcga gcggtaatcg atcaccacat cgccggtcgc
     8581 ggcaaccagg aagtagcggt tgggtcgggt gatcgtcggg gtgtcgatcg cttcgagttc
     8641 cgtcaggtac tcgcggcgga actcgatctg cgcgtcggaa aaatagactg gctgtacgcc
     8701 taaatgacga gccagatcat cctgcgggcg gatggcagga ttcagcaaca ccgcgcgaca
     8761 gcccagccgc tccgccatcc aggtcgcgta gtacccgccc agcgaggaac cgatcagcgc
     8821 gacacgtgcc ggcgcaatcc ccgcaacagc gctctgcgcg acctcgatgg cccgggccgg
     8881 cgaggccggc agcgcaggac agacatagtc ggcggatcgg ccgcgccggt ccatcgcttc
     8941 acgcagcatc gtggccttgc gagatgccgg cgatgatcga aaaccgtgaa ggtagacaat
     9001 cacgcttctg ccgcccggct cgccaggcgg tccagcagac gctcgtgaat cccaccgaag
     9061 ccaccattgc tcattaccac gacacgatcg cccgcacgcg cgcaggccac cacgtcgtcg
     9121 accagtgcct gcaactccgc atgaatcgat gcccgttcgc cgagcggagc aagtgcctcg
     9181 acaacattcc agccgacgct ccctgcatag cagaacacgc gatcagctcc ttgcaggctc
     9241 ggcgccagct cggccttcac ggcgccctgc ttcatcgtgt tcgagcgggg ctcgagtacc
     9301 gcgatgatgc gcgcgtcgcc gacctcttcg cgcaacgcgt cgatcgtctc gcgaatcgcc
     9361 gtgggatggt gcgcaaagtc gtcgatgact gtgaccccgg caaccgtccc gcggacctcc
     9421 agccgccgct tgaccccgcc aaaactcgcg agcgcttcca gagcgtcggc cgggccgacg
     9481 ccaacatggt gagcagcgac gatggccgcc agcgcgttca tgcggttgtg ttcacccgga
     9541 agcaccagcc gcagcgaccc gacgatgcgg ccgtcatgca ccacctcatg cggcgcgccg
     9601 ggaccgacgt gccaccgttc gccgccgaat ctctcgactt cgctccagca gccccgcgcc
     9661 agaacgcgct cgagtgccat gttccgctcg ttgacgatca gcctggctgt cgccggcagt
     9721 gtccgaacca ggtggtgaaa ctgggtttcg atcgctgcca gatccgcgaa gatgtccgcg
     9781 tgatcgaact cgagattgtt caggatggtc gtacgcgggc ggtaatgaat gaacttggac
     9841 cgcttgtcga agaacgccgt gtcgtactca tccgcctcga tcacgaagaa gcggctggcc
     9901 gtcagccgtg cggatacgcc gaagtcggtc ggcacgccgc cgaccaggaa ccccgggtcc
     9961 atctgcgcat gatcgaggat gcacgtcagc atcgccgtgg tcgtcgtctt gccatgggtg
    10021 cccgcgactc cgagcacgtg gcgcccggcc agcacatttt cggcaagcca ttggggtccg
    10081 gaggtgaacg gcagcccgcg ctccaggatc tcttccatca gcggattgcc tcgcgtgaca
    10141 acattgccga tcacccacag atcgggcgcg atgccgatct gctcggcgcc gtagccttcg
    10201 atcagtcgca cgcccgccgc cgccagctga tcgctcatcg gcgggtagac actggcgtcg
    10261 cagcccgaca cgcgatggcc cgcagcgagc gccagttgcg ccagaccacc catgaaagtg
    10321 ccgcagatgc cgaggatatg gatgtgcacg aggaatcgaa cgaagcgggc ggcgtggagt
    10381 cgtagcgctg acggccgcga aggcgcgcga ttgtaatgcg cggcgctccg acagccgccc
    10441 gctagacttc ccggccaaca tgacctcaag caatgaatca ctcagaaccg aactggcggc
    10501 cgcggcggcc cgcctcatcg cggaggaggg ttgcgactac ggccaggcca agcggcgtgc
    10561 attgcatgac ctgcttggcg atacggggtc ccgacgcctg ctgccggaca acgccgagat
    10621 cgaacacgag ctgcgccggc acctgcagct ctttgccgca gacacgcatc ccgcattgct
    10681 cgcggccttg cggcgttctg ctgccggcgt aatggcacgc ctggctgact tcaatccgca
    10741 cctggtcggt gcggtgctga gcggcacggc caccgagcac tccgacatcg agttacatct
    10801 gttcactgac agcgccaagg atgtcgaggt ggttctgatg gagagtcgca tcgagttcga
    10861 ggccgaagcc gcggacgacc acgatcgtcc cttgcccctc gagcgattga cgtttgtcct
    10921 gcccgcccgg gaacccggat tgccgccgtc actgcgcctc atcggcgtgc gcctccacgt
    10981 ctatgaccgc gatgcgatcc gagtcgcgcc gaggcatcgc gcccttgcga cgtccgagtt
    11041 cgatgtccat ccgatcgccg ccggaggccg ggccaacctg gctgcccttc ggcagttgat
    11101 cgaggactcc gagccatgaa cgcgtcacgt cgccgcctgc tgttcgccgg tgtcggtgcc
    11161 atcgcgctcg cgggcggtgc cgggctcgct gtgtaccgcc ttcgaccgga ccctgtcggc
    11221 gccgcggcgg tggcgacgct gttcgcgcaa tcgttcgacg acgcccaggg tcaaccgcag
    11281 ccgatggtgc aatggaaggg acagtggctc gtcctcaatt tctgggccac ttggtgcacc
    11341 ccctgcgtcg aggagatgcc aaccctgcag caggtggcgc gggactacgg cagccgcggc
    11401 gtcgccgtgg tcgggctggg gatcgacagt cccgccgcga ttcgtcgctt ccggtcggag
    11461 ctcaaactgg aactgccgtt gctggttgcc ggagccggtg gaactgagct tgcccgggag
    11521 ctcggcaatc cgagcggcgc gttgccgtac actgtgctga tcagtccgaa gggtgctgtc
    11581 gaacaggccc atttgggcct gctcaggccc gaactgctcc gatcttggct cgacgcccga
    11641 ttgagctcct gagtggccgc tggacatcgg cgttcggatg gttcagactc cgcgccgccc
    11701 cgtgattcgg ccgcatcttc tgccatttcc gccgaaaagt aaccaaaacc gcctaggagc
    11761 aatgtgtcaa gtcgcattct cgttctccat ggcccgaatc tgaacctgct gggaacgcgc
    11821 gaacccgcca tctatggcgg acgcaccctg gcgcagatcg agtccgaact ctccgacgtt
    11881 gcgcgcagat acgcggcaga tctcgttttt ttccag
//
LOCUS       DATIRT010000042         4228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_201421, whole genome shotgun sequence.
ACCESSION   DATIRT010000042 DATIRT010000000
VERSION     DATIRT010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201421"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1665)
                     /locus_tag="VLN25_00940"
     CDS             complement(<1..1665)
                     /locus_tag="VLN25_00940"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HSQ09172.1"
                     /translation="MNFFEHQELARRNSQRLIVLFVLAVVAIVIAVNLAATFVYHVFV
                     LPAGARWMSSQLPNGFYFTNTVVVLGLILGGTWLEMNRLKAGGSAVAQMIGAREVDTS
                     TRDLLDRRLVNVVEEMAIASGVAMPRVFVMDNENAINAFAAGHSVNDAIVAVTRGTLT
                     RLSRDELQGVIAHEFSHILNGDMKLNLRLIGVLYGLLIVALAGRFLLEIGGRSRGGGR
                     NSGSAIAVLFAAGLALWILGYIGVFFGRLIKAGVSRQREFLADASAVQFTRNPDGIGG
                     ALRKIAGLTEQTGLGTRIDHPQAESLSHLFLGAARPSFARGLFATHPPLDDRVQRIFG
                     RALPALPAPENAVALALGGLSPSSEPDRPRSPIQFVPSARSDAATLTRAEALPASSSP
                     VAGLVAASANAAASIATDIGTVRPPGHRDFALDEAQRGLVESLRRAATDGMRAQLLVL
                     SFLVDKDRELRGQQRALIAEAYGGEAAQIADGYHEQVQCLPPGARQPLVDVAMPALRK
                     LPDDARESLLRTAHLLIAADGRLTVREFLLFTILKRRLGRDAGHPVP"
     gene            complement(1746..2333)
                     /locus_tag="VLN25_00945"
     CDS             complement(1746..2333)
                     /locus_tag="VLN25_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HSQ09173.1"
                     /translation="MGWIIVLVIIAVVVFWAVGAYNRLVSLRNKFKNAFAQIDVQLKR
                     RYDLIPNLVETAKGYMKHERETLESVIAARNTAVTANAAAAADPGSANAMQGLIAAEG
                     ALSGALSRLFALVEAYPDLKANQNMMQLTEELTATENKISFARQAFNDSVMSYNTASQ
                     QFPTNVIAGMFSFSAAELLQATESDDERKPVRVQF"
     gene            complement(2598..3257)
                     /locus_tag="VLN25_00950"
     CDS             complement(2598..3257)
                     /locus_tag="VLN25_00950"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HSQ09174.1"
                     /translation="MKQIFVRATCAALLASTAVACAPIVVGVVAAGTALVVTDRRTTG
                     SQVDDQTIQLRVANELGAAFKNQPVHINVNSFDRKVLLTGEVPDEAARTRAGAIATAT
                     QNVRMVVNELAAAPPSSMSDRTSDTALSGRVRAAFINTREIAFNSVEIVTERRIVYLL
                     GLVTDEEGKIAALVASKVSGVQQVVKVFDIASREEVDRRRAGGTSVAPASPPASPAVT
                     K"
     gene            complement(3272..3670)
                     /locus_tag="VLN25_00955"
     CDS             complement(3272..3670)
                     /locus_tag="VLN25_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008333580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HSQ09175.1"
                     /translation="MATAGGRARRVAPGVRTPKQRAGDAAESAAARYLIAQGCDLIAR
                     NARYPEGELDLIMRERDVLVFVEVRMRGSRAYGGAAGSVDRYKQKRLLRAAQHWLLQH
                     CGERWPACRFDVVSVDGDGTIEWIRDAFAA"
     gene            complement(3714..>4228)
                     /locus_tag="VLN25_00960"
     CDS             complement(3714..>4228)
                     /locus_tag="VLN25_00960"
                     /inference="COORDINATES: protein motif:HMM:NF016251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HSQ09176.1"
                     /translation="VPQLVEWTPATAGKVAAQLTTSTLEAVFLALTARDAAQMRALIP
                     RQVQIFGTSLLYIGDPRTSPDAATLAHDLEGVRFVDMPWLLEPDHAGVMVYPAPANPL
                     PTELVRLYALGIDAYRLATVWMKGERRFDLDGVTGRLQVDRARSLRVERTPVLAIYRG
                     GAVQRIEVAR"
BASE COUNT          748 a         1452 c         1356 g          672 t
ORIGIN      
        1 cggcaccggg tgaccggcat cgcgaccgag ccgccgcttc aggatcgtga acaggagaaa
       61 ctcgcgcacg gtcagtcggc cgtcggcagc gatcagcaga tgcgccgtgc gcaacaggga
      121 ctcgcgcgca tcgtccggta acttgcgcag cgcaggcatc gcgacatcga ccaggggctg
      181 acgcgcgccg ggcggcagac actggacctg ctcgtgatag ccgtcggcaa tctgcgcggc
      241 ttcgccgccg taggcctcgg caatcagggc ccgctgctgc ccacgcagtt ctcggtcctt
      301 gtccaccaga aaggagagca cgagcagctg cgcgcgcatc ccgtcggtgg ctgcgcggcg
      361 cagtgactcc accaggcccc gttgcgcctc gtccagcgca aagtcgcgat gccccggcgg
      421 tcgtaccgtg ccgatatcgg tcgcgatgga cgccgccgcg ttcgcactgg ccgccaccag
      481 gccagccacc gggctgctgc tcgcgggcag tgcttcggca cgcgtcagcg tcgcagcgtc
      541 ggaccttgcc gacgggacga actggatcgg cgatcgcggc cgatcgggct ccgacgaggg
      601 gctgagaccc ccgagcgcca gggccaccgc gttttccggg gccggcagcg ctggcaatgc
      661 gcgcccaaag atgcgctgca cgcgatcgtc cagcggcgga tgcgttgcaa acaggccgcg
      721 cgcgaaactc ggccgtgccg cgccgagaaa aaggtgcgac agagattcgg cttgcgggtg
      781 atcgatgcgc gtgcccagcc cggtctgctc ggtcaatccg gcaatcttgc gcagcgcgcc
      841 gccgatgcca tcgggattac gcgtgaattg caccgcgctg gcatcggcga gaaactcgcg
      901 ctgccgcgac acaccggcct tgatcagccg accgaagaac acgccgatgt atccgaggat
      961 ccatagcgcg aggccggccg cgaacagtac ggcgatcgcg ctgccgctgt tgcggccgcc
     1021 gccgcggctg cgtccgccga tctccagcag gaatcgtccc gcgagcgcga cgatcagcag
     1081 cccgtagagc acaccgatga ggcgcagatt gagcttcatg tcgccgttca ggatgtgcga
     1141 gaactcgtgt gcgatcacgc cctgcagttc gtcgcgggag agccgggtca gcgtgccgcg
     1201 agtcacggcc acgatcgcat cgtttacaga gtgcccggcc gcaaaggcat tgattgcatt
     1261 ttcgttgtcc atcacgaaca cgcggggcat ggcgacaccc gacgcgatcg ccatttcctc
     1321 gacgacgttc accagacgcc ggtcgagcag atcccgcgtc gacgtatcga cttcgcgcgc
     1381 gccgatcatc tgggccaccg ccgagccacc ggccttcagg cgattcatct cgagccatgt
     1441 tccgcccagg atcaggccga gcacgaccac cgtgttcgtg aagtaaaagc cgttgggcag
     1501 ctgtgacgac atccagcgcg ccccggccgg caggacgaac acgtgataca cgaacgtggc
     1561 cgccaggttc accgcgatca cgatcgcgac cacggccagc acgaacagca cgatcagccg
     1621 ctggctgttg cgccgtgcaa gctcctggtg ctcgaagaaa ttcatgagga ccgtgtcgcg
     1681 acgcgcgggg tgggcgagtc agtgcggtcc gcgccgcagc acggaccgac aacgggatcc
     1741 gtcgatcaga actgcacgcg gaccggcttg cgctcgtcgt cgctttcggt cgcctgcagc
     1801 agttccgcgg cactgaacga gaacatgccg gcgatcacat tggtcggaaa ctgctgcgac
     1861 gccgtgttgt agctcatcac cgagtcgttg aacgcctggc gcgcgaacga gatcttgttc
     1921 tcggtggcgg tcagttcctc ggtcagctgc atcatgttct ggttggcctt caggtccgga
     1981 taggcctcga ccagcgcgaa caggcgcgac aacgcgcccg acagcgcgcc ttcggcggcg
     2041 atcagtccct gcatcgcgtt cgcgctgccg gggtcggccg ctgccgcggc attggccgtc
     2101 accgcggtgt tgcgggcagc gatgacactt tcgagtgtct cgcgctcgtg cttcatgtag
     2161 cccttcgcgg tctcgaccag gttcggaatc aggtcgtagc gccgcttgag ctgcacgtcg
     2221 atctgcgcaa aggcgttctt gaacttgttg cgcaggctga ccagccggtt ataggcaccc
     2281 accgcccaga agacgacgac ggcgatgatg accagcacga tgatccagcc cattgcaccc
     2341 tccgtgagga atctcagcgc ggctgacgag ccaaccggca cgtcaggagc ggcgcactat
     2401 aagtcaaggg gccggcgatg caatgggtga agtgaccgtc gatcgccgaa tatccgccgg
     2461 tgatggccag caatgctcca gcagtcctca ccagcaatgc tcctctacgg cttgcgcaac
     2521 acaatgtcgc cgagtgccga gtgctgcgct acccgcgcgc agcgcccgga tcacacacga
     2581 cagcaggctg ctgttgccta cttcgtcacc gcgggcgagg ccggcggtga cgcgggtgca
     2641 actgaggtgc ccccggcgcg acgccggtcg acctcttcgc gcgaggcgat atcgaacacc
     2701 ttgacgacct gctgcacacc cgacaccttg ctcgccacca gggccgcaat cttgccttcc
     2761 tcatcggtga cgagaccgag caggtagacg atgcgccgct cggtgacgat ttcgacggaa
     2821 ttgaaggcga tctcgcgcgt gttgatgaac gccgcacgca cacgacccga cagtgccgtg
     2881 tcggacgtgc gatcagacat cgacgacggc ggcgccgcgg caagttcgtt cacgaccatg
     2941 cgcacgttct gcgttgccgt cgcaatggcg cccgcgcgcg tgcgggcagc ctcgtccggc
     3001 acctcgcccg tcagcagcac cttgcgatcg aaactgttca cgttgatgtg caccggctga
     3061 ttcttgaacg ccgcacccag ttcattggcc acccgcaact ggatggtctg gtcgtccacc
     3121 tgcgagccgg tcgtgcgccg gtcggtcaca accagtgcgg taccggccgc caccacgccg
     3181 accacgatcg gcgcgcacgc cacggcggtc gaggccagca gcgcggcgca cgtcgcgcgt
     3241 acgaagatct gtttcatgtc tgctcgaact cctatgccgc gaaagcgtcg cggatccatt
     3301 cgattgtgcc atcgccgtcc acgctgacga cgtcgaaccg gcaggcgggc cagcgctcgc
     3361 cgcaatgttg cagcagccag tgctgggccg cgcgcagcag gcgcttctgc ttgtagcgat
     3421 caacgctgcc ggccgcaccc ccataggcac gactgccccg catacggact tcgacaaaca
     3481 ccagcacgtc gcgttctcgc atgatgaggt cgagttcacc ctcgggatag cgcgcattgc
     3541 gagcgatcag atcgcacccc tgggcgatca gataccgggc cgcggccgac tcggccgcat
     3601 cgcctgcacg ctgcttcggg gttcgaacgc ccggagcgac gcggcgcgca cggcctccgg
     3661 ccgtcgccat caggccggca ccatcaacgc ggacacgtca gcgcagcaca cgttcatctg
     3721 gctacctcga tgcgctggac cgcgccaccg cgataaatcg ccagtaccgg cgtgcgttct
     3781 acccgcagcg acctggcgcg gtccacctgc aaccggccgg tgaccccgtc gagatcgaag
     3841 cggcgttcgc ctttcatcca gaccgtcgcc agccgatagg cgtcgatgcc gagcgcatac
     3901 aggcgcacca gttcggtcgg cagcggattc gccggcgccg gatagaccat gactccggcg
     3961 tgatccggtt ccagcagcca cggcatgtcg acgaagcgca cgccttcgag gtcatgcgcc
     4021 agcgtggcag cgtccggcga agtacgcgga tcgccgatat acagaagaga ggtaccgaag
     4081 atctggacct ggcgcggaat cagcgcgcgc atctgcgcgg cgtcgcgcgc cgtcagggcg
     4141 agaaaaacgg cttcgagcgt cgacgtcgtc agctgcgccg ccacctttcc agcagtcgca
     4201 ggagtccact cgaccagttg gggcactt
//
LOCUS       DATIRT010000043         1873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_791344, whole genome shotgun sequence.
ACCESSION   DATIRT010000043 DATIRT010000000
VERSION     DATIRT010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791344"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..>1873)
                     /locus_tag="VLN25_00965"
     CDS             complement(47..>1873)
                     /locus_tag="VLN25_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSQ09177.1"
                     /translation="HIGPMTIAIVLIAVGVVSLGLWRGRRDSELFMLLAIGSLLWALQ
                     ILMYQWPTRFLPRPHWSVFVFSIYAWFPALIGVFFLRFAYRRSALLEWFAVVLAVVAA
                     PMMYAGFQFDLVSQASIALRVSVLVFITIALIALLRYALQLRTLTGYTLFSMGAICVG
                     VAFRDFVLSLSPNSYEMLILNPYSGVALLLFAGWMLLERYHKAFADFEMLNLDLEKRV
                     DAANVELHRRLEQVESARAAAEQANVAKSRFFAAASHDLRQPLHSLGLFATALRDIVG
                     NTEGRDLVQRIGDSIGALDRLFDELLDISRLDAGTVDVRCRNIALQPLFDRLGGDFFA
                     EAAAKDLRLRFHPTALAVSTDPLLLERVLANLVANAVRYTRAGGVLVGARRRGAQVML
                     QVWDTGIGIPEDQRSLIFDEFYQVSNPGRDRRKGLGLGLAIVRRLTTLLEHPLSLRSI
                     ESRGTCFTLTLPLATGPIEAPSAEESGERFELFTGLRALIVDDDADICDATVRLMTRW
                     GFDARAAPRYAAARELLQGGFLPDVILADLRLGEVIDGIGVVERLRVQLNDTVPALLI
                     SGDTGARELARVKQSGLLLLTKPVAPARLRSALHALLLATQR"
BASE COUNT          375 a          621 c          618 g          259 t
ORIGIN      
        1 cgccacgctc gtgctgccgc gcccgacaga gcgctagttc agcggcctat cgctgcgtcg
       61 ccaagagcag cgcgtgcaat gcgctgcgca gccgggccgg cgccaccggc ttggtgagca
      121 gcagcaaccc gctttgcttc acgcgcgcca actcgcgcgc cccggtgtcc cccgaaatca
      181 gcagcgcggg caccgtatcg ttcagttgga cgcgcaaccg ctcgaccacg ccgatgccgt
      241 cgatcacttc ccccaggcgc aggtcggcca ggatgacgtc cggaagaaat ccgccctgca
      301 gcagttcgcg ggcggcggca tagcgcggcg cggcgcgcgc gtcgaaaccc cagcgcgtca
      361 tcagccgcac ggtggcatcg cagatgtcgg cgtcgtcgtc gacgatcagg gcgcggaggc
      421 cggtgaacag ctcgaaacgc tcgccggact cctcagcgct cggtgcttcg atcggtcccg
      481 tcgcgagcgg taacgtcaac gtgaagcacg tgccgcgact ctcgatcgag cgcaggctca
      541 atgggtgctc gagcagggta gtcaggcgcc gcacgatggc cagtccgagc ccgagccctt
      601 tgcgccggtc gcgcccggga ttactcacct gataaaactc gtcgaagatg agcgagcgct
      661 gatcctcggg aatgccgatg ccggtatccc agacctgcag catcacctgc gcaccgcggc
      721 ggcgcgcgcc caccagcacg ccgcccgcgc gtgtatagcg gactgcgttg gcgacgaggt
      781 tggccaacac gcgctccagc aggagcggat ccgtgctcac cgcgagcgcg gtcggatgga
      841 accggagccg cagatccttc gccgccgctt cggcaaagaa gtctccgccc aggcggtcga
      901 acagaggctg cagggcgatg ttgcggcacc tgacatcgac cgttcccgcg tcgaggcgcg
      961 agatatcgag gagttcgtcg aacagccggt cgagtgcacc gatcgagtcg ccgatacgct
     1021 gcaccaggtc gcgcccttcg gtgttgccga cgatgtcgcg caaggccgtg gcaaacagcc
     1081 ccagcgagtg cagcggctgg cgcagatcgt ggctggcggc ggcaaagaag cgcgacttgg
     1141 ccacgttggc ctgctcggcc gcggcgcgtg ccgactcgac ctgttcgagt cgccggtgca
     1201 gttcgacatt ggcggcgtcg acacgcttct ccaggtcgag attcagcatc tcgaagtcgg
     1261 cgaaggcctt gtggtagcgc tcgagcagca tccatcccgc gaagagcagc agagcgacac
     1321 cggagtaggg attgaggatc agcatttcat aggagttggg ggatagcgac agcacgaagt
     1381 cgcggaacgc gaccccgacg cagatggccc ccatcgaaaa cagcgtgtag ccggtcaacg
     1441 tgcgcagctg cagggcgtac cgcaatagcg cgatcagcgc gatggtgatg aataccagca
     1501 cggaaacccg caaggcgatg gacgcctgcg acacgagatc gaactggaat ccggcataca
     1561 tcatcggcgc ggcgaccacc gccaatacca cggcgaacca ctccagcagc gctgaccgcc
     1621 gataggcaaa tcgcaggaag aacacaccga tcagtgcagg gaaccacgca tagatcgaga
     1681 aaacgaacac cgaccagtgg gggcgcggca ggaatcgcgt cggccactga tacatcagga
     1741 tctgaagcgc ccacagcagc gagccgatgg ccaggagcat gaacagttcg ctgtcgcgcc
     1801 ggccgcgcca caggccgagc gagaccacgc ccaccgcgat cagcacgatg gcgatggtca
     1861 tcggaccgat gtg
//
LOCUS       DATIRT010000044         2775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1479789, whole genome shotgun sequence.
ACCESSION   DATIRT010000044 DATIRT010000000
VERSION     DATIRT010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1479789"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VLN25_00970"
     CDS             complement(<1..567)
                     /locus_tag="VLN25_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HSQ09178.1"
                     /translation="MPRPAPCLKRAAGVVLVALLAACSTTPPGSAPTKKGAYYQDDGP
                     PERVPADIASIPDAVPRVEPPHRAASRSYVALGRTYTPLTGDVSFRQRGMASWYGRQF
                     HGNRTANGEIYDMFGMTAAHPILPLPSYVRVTNIRNGRSVIVRVNDRGPFKHDRVIDL
                     SYAAAAKLGIAAAGTGEVEVERITMTQIA"
     gene            complement(567..1685)
                     /gene="rodA"
                     /locus_tag="VLN25_00975"
     CDS             complement(567..1685)
                     /gene="rodA"
                     /locus_tag="VLN25_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HSQ09179.1"
                     /translation="MNEATLGRRVGLLVRPYVTVFDPSLLAIVGCLGVIGLITLYSAG
                     NDFPWRVTDQMRNFGVALAVMFVAANISPQVLMRLAVPIYLAGVGLLVATEVAGITVK
                     GATRWLDIGVGRIQPSEIMKLGLPMMLAWYFHKREGGLRSYDFVIAAVIVLVPVALII
                     KQPDLGTALLVLAAGTYVIFFAGLPWKWLLAMIAAGIAALPLAWTLLHDYQRERILTL
                     IDPSTDPLGKGFHTLQAMIAIGSGGVFGKGWMQGTQTHLEFIPERTSDFIFAVYSEEF
                     GLVGNLVLVLLFFALVWRAMAIALNAPTQFARLLAAALGLIIFTYAFVNMGMVSGILP
                     VVGVPLPFISYGGTALVTLGMACGMLMSIGANRKLVQS"
     gene            complement(1682..>2775)
                     /locus_tag="VLN25_00980"
     CDS             complement(1682..>2775)
                     /locus_tag="VLN25_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HSQ09180.1"
                     /translation="LAFVSMPNFDPNLFVDGIDPKSWDELNNDPDKPLLNRALRGTYP
                     IGSTYKPFLALAALETGKRRTDTVIHDGGTFVFGNRVFRDSNKVPLGAVDMHRSIVKS
                     SDIYYYQLANDLGVDAIHDFMKPWGFGQITGIDVEGEQTGILPSTAWKERRFKQKWYP
                     GETISIGIGQGYNSFTLLQLAHATANLANDGVVMKPHLVKEVEDARTGEKRLTVPKES
                     YRIPVKPEYMKFVRDAMVDVNISGTGRVAFQGAPYKVGGKTGTAQVISIKQGEKYNEA
                     RVAERHRDHSLFIAFAPADDPKIAIAVLVENGGFGARAAAPIARAAIDYYLIGKKPAG
                     PAATDDGPDDEEVPRTVAAAASSPARSVR"
BASE COUNT          505 a          920 c          879 g          471 t
ORIGIN      
        1 ggcgatctgc gtcatggtga tgcgctcgac ctcgacttct ccggtccccg cggcagcaat
       61 gccgagcttg gccgctgcgg cgtacgacag gtcgattacg cggtcgtgtt tgaagggccc
      121 tcgatcgttg acacggacga tgacgctacg gccattgcgg atgttggtga cgcgcacata
      181 gctcgggagc ggcaggatcg gatgtgccgc agtcatgccg aacatgtcgt agatctcgcc
      241 gttcgcggtg cgattgccat gaaactgccg gccgtaccac gacgccatgc cgcgctggcg
      301 aaacgacaca tcaccggtca gcggcgtata ggtgcggccc aatgcgacgt atgaccggct
      361 tgccgcccgg tgcggcggct cgacgcgcgg cacggcgtcc ggtatcgacg cgatgtcggc
      421 cggcacgcgc tcgggcgggc catcgtcctg gtagtaggcg cccttcttcg tcggcgccga
      481 gcccggtgga gtcgtactac aggccgccag cagcgccacg agcaccacgc ccgccgcccg
      541 cttcagacac ggcgccgggc gcggcatcag ctctggacca gcttgcgatt ggcaccgatc
      601 gacatcagca ttccgcacgc catcccgaga gtgaccagcg cggtgccgcc atagctgatg
      661 aagggcagcg gcacgcccac gaccggcagg atgccgctca ccatgcccat gttgacgaac
      721 gcataggtga agatgatcaa cccgagcgcg gccgcaagca atcgcgcgaa ctgcgtcggc
      781 gcgttgagcg cgatcgccat cgcacgccag accagtgcaa agaacagcag caccagaacg
      841 agattgccga cgagcccgaa ttcctcggag tacaccgcga agatgaagtc gctggtgcgt
      901 tcggggatga actccaggtg cgtctgcgtg ccctgcatcc agcccttgcc gaacacgccg
      961 cccgagccga tcgcaatcat cgcctgcagc gtgtggaagc ctttgccgag cgggtccgtg
     1021 gacggatcga tcagtgtcag gatgcgctcg cgctggtagt catgcagcag ggtccatgcc
     1081 agcggcaacg cggcgatgcc ggcggcgatc atcgcaagca gccacttcca cggcagaccc
     1141 gcgaagaaga tcacgtacgt ccccgctgcc agcacgagca gcgctgtccc gagatcgggc
     1201 tgcttgatga tcagcgcgac cggcaccagc acgatgacgg ccgcgatgac gaaatcgtac
     1261 gaacgcaacc cgccctcgcg cttgtggaag taccacgcca gcatcatcgg cagcccgagc
     1321 ttcatgatct ccgatggctg gatgcgcccg acgccgatgt cgagccagcg cgtcgcgcct
     1381 ttgacggtga tcccggccac ctcggtggcg actagcagtc cgacaccggc caggtagatc
     1441 ggtaccgcga gccgcatcaa cacctggggc gagatgttcg cggcgacaaa catcaccgcc
     1501 agcgcaacgc cgaagttacg catctgatcg gtcacgcgcc agggaaagtc attgccggcc
     1561 gaatacagcg tgatcagccc gatcacaccc aggcagccga cgatcgccag cagcgatgga
     1621 tcgaacaccg tcacgtaagg gcgcaccagc agcccgacgc gtcgaccgag cgtggcttcg
     1681 ttcaccgcac gctccgtgcc ggactggagg ccgccgccgc gacggtgcgc gggacttcct
     1741 cgtcgtcggg tccgtcgtca gtggccgccg gtccggctgg cttcttcccg atcaggtagt
     1801 agtcaatcgc agcccgcgca atcggtgccg cggcacgggc gccgaatccg ccgttttcca
     1861 ccagcaccgc aatcgcgatc ttcgggtcgt cggccggcgc aaaggcgatg aatagcgagt
     1921 gatcgcgatg gcgttcggcc acgcgcgcct cgttgtactt ctccccctgc ttgatgctga
     1981 tcacctgggc cgtgccggtc ttgccgccga ccttgtacgg tgccccctga aacgcgaccc
     2041 gacccgtgcc gctgatattg acgtcgacca tcgcgtcgcg cacgaacttc atgtactcgg
     2101 gcttgaccgg gatgcggtag ctctccttcg gcacggtcag tcgcttctct ccggtgcgcg
     2161 cgtcctcgac ctccttgacc aggtgcggct tcatcacgac accgtcgttg gcgagattcg
     2221 ccgtagcgtg cgccagttgc agcaaggtga acgagttgta accctggccg atgccgatgg
     2281 agatcgtctc gcccggatac cacttctgct tgaagcgacg ttccttccag gcagtcgacg
     2341 gcaggatgcc ggtctgctcg ccctcgacgt cgatgccggt gatctggcca aatccccagg
     2401 gcttcatgaa atcgtggatc gcgtccacgc ccagatcgtt agcaagctgg tagtagtaga
     2461 tgtcgctcga cttgacgatc gagcggtgca tgtcgaccgc ccccaacggc accttgttcg
     2521 agtcgcggaa cacgcggttg ccgaagacga aggtgccgcc gtcgtggatc accgtatcgg
     2581 tccggcgctt gccagtctcg agcgccgcca gcgcaaggaa cggcttatag gtcgaaccga
     2641 tcggataggt gccacgcagc gcgcggttga gcaggggctt gtccgggtca ttgttcagct
     2701 cgtcccagct cttcgggtcg atgccgtcga caaagagatt agggtcgaag ttcggcatcg
     2761 agacgaaggc aagca
//
LOCUS       DATIRT010000045         1758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_988754, whole genome shotgun sequence.
ACCESSION   DATIRT010000045 DATIRT010000000
VERSION     DATIRT010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988754"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1306)
                     /locus_tag="VLN25_00985"
     CDS             complement(<1..1306)
                     /locus_tag="VLN25_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008542682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HSQ09181.1"
                     /translation="MTLGQLEPNQTAPGGTAPQDAGSTRALVEPLYAGKSLRTGEPFI
                     AHADGIASIVATLRSDPDLLAAAQLFGIYDVLRDPDDWLRSRFGPTVAQLVADLRQLM
                     RLSELTRTREEARGRDPGDQTEALRRMLLAMANDLRVVLLRLASRLQTLRYYALSKHV
                     GAEPFARETLELYAPLANRLGIWQIKWELEDLSFRFLQPETYKAVARMLDEKRAERES
                     FIDSALARLRAMVADAGLRAEISGRPKHIYSIWNKMRAKGLAFEQVYDVRGLRVIVDE
                     VAQCYQVLALVHSEWNPVESEYDDYIARPKPNGYQSLHTVVGDDAGRTLEVQIRTRRM
                     HEHAELGVAAHWRYKEGGKGRDDDELRVAWLRQLLAWRAEVDAPLASGPAREERIYVL
                     TPQARVIELPAGGTPIDFAYHIHSELGHRCRGARVDGVMVPLS"
     gene            1360..>1758
                     /locus_tag="VLN25_00990"
     CDS             1360..>1758
                     /locus_tag="VLN25_00990"
                     /inference="COORDINATES: protein motif:HMM:NF017932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HSQ09182.1"
                     /translation="MLRALIGRISGPQVAIPDLLWDATLASMPFLARLTIGDNGRLRA
                     LVCQFLADKEMAGAAGLELTSGMQVNIAVQACLPVLNLGLDWYRGWCSIVVYPGEFLV
                     PRSVTDDAGVVHEYSEPIAGEAWDGGPVLIS"
BASE COUNT          286 a          581 c          572 g          319 t
ORIGIN      
        1 tgctcagcgg aaccatgacc ccgtcgacgc gcgcgcctcg gcagcgatgt cccagttcgg
       61 agtggatgtg gtacgcgaag tcgatcggtg tgccaccggc gggcagctcg atcacgcgcg
      121 cctgcggcgt caggacatag atgcgctcct cgcgtgccgg cccgctggcc agtggcgcat
      181 cgacctcggc tcgccaggcg agcagctggc gcaaccaggc gacccgcagt tcatcgtcgt
      241 cgcgcccctt gccgccttcc ttgtagcgcc agtgggcggc cacgccgagc tcggcgtgct
      301 cgtgcatgcg tcgggtcctg atctggacct cgagcgtgcg gccggcgtca tcgccgacaa
      361 ccgtgtgcag cgactgatat ccattgggct tgggtcgcgc gatgtagtcg tcgtattcac
      421 tctcgaccgg attccactcc gagtgcacga gcgccaggac ctgatagcac tgagcgactt
      481 cgtcgacgat gacgcgcagt ccccgcacgt catagacctg ctcgaaagcg agtcccttgg
      541 cgcgcatctt gttccagatg ctgtagatat gcttggggcg cccgctgatc tcggcgcgca
      601 gcccggcgtc ggccaccatc gcgcgcaggc gagccagagc tgaatcgatg aacgactccc
      661 gctcggcgcg cttctcgtcg agcatgcgcg caaccgcctt gtaggtttcc ggttgaagaa
      721 agcggaacga caggtcctcg agttcccact tgatctgcca gattccgaga cggttggcca
      781 gaggcgcata cagctcgagc gtctcgcgtg cgaagggctc tgccccgacg tgcttgctga
      841 gcgcgtagta gcgcagcgtc tgcaagcgtg acgcgagcct caacagaacc acgcgcagat
      901 cattggccat cgccagcagc attcgcctca atgcttcggt ctgatcgccc ggatcacgac
      961 cgcgcgcttc ctcgcgcgta cgcgtcagtt ctgagagccg catcagctgc cgcagatcgg
     1021 ccacgagctg cgctaccgtc gggccgaagc ggctgcgcag ccagtcgtcc ggatcgcgca
     1081 gcacgtcata gatgccgaag agctgcgccg ccgcaagcag atcagggtca gaccgaagtg
     1141 tcgcgacgat ggaggcgatg ccgtccgcat gggcaatgaa cggctcgccg gtgcgcaggg
     1201 acttgcctgc atagagcggt tcgaccagtg cgcgcgtact gcccgcatcc tgcggtgcgg
     1261 ttccgccggg ggcggtctgg ttcggttcga gctgtcccaa ggtcatcacg gttcgattat
     1321 cccgcctcgg cacccagacc attgcggcac aattccgcga tgctgcgggc actgatcggt
     1381 cggatctccg gacctcaagt tgcgattccc gacctactgt gggacgcaac cctcgcgtcg
     1441 atgcccttcc tcgcgcgcct gacgatcgga gacaacgggc gccttcgtgc actcgtctgc
     1501 cagttcctcg ccgacaagga gatggcgggt gcagccggac tggagctgac gtccgggatg
     1561 caggtgaaca tcgcggttca ggcctgcctg ccagtcctga atcttgggct cgactggtac
     1621 cgcggctggt gcagcatcgt cgtctatccg ggggagttcc tcgttccgcg tagcgtcacg
     1681 gacgacgcag gcgtcgtgca cgagtactcc gaaccgatcg cgggcgaagc gtgggatggc
     1741 ggcccggtgc tgatctca
//
LOCUS       DATIRT010000046         4461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1480786, whole genome shotgun sequence.
ACCESSION   DATIRT010000046 DATIRT010000000
VERSION     DATIRT010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480786"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VLN25_00995"
     CDS             <1..369
                     /locus_tag="VLN25_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HSQ09183.1"
                     /translation="HGPGPYVMGADTLVGNDVYNRDSQDLGDIKEIMLDMRTGRVSYA
                     VLSFGGFLGMGEKLFAVPWDALTLDTENKRFVLDVDKTRLQGAPGFDKNHWPDMADAT
                     WEEGIHAYYGTKPYREQPRV"
     gene            506..1318
                     /locus_tag="VLN25_01000"
     CDS             506..1318
                     /locus_tag="VLN25_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HSQ09184.1"
                     /translation="MQIRVGFEMEYQCRGPTPMVLALSIHHSRASDLVRPDLLVTSPA
                     VTVTAYRDLFGNWCSRLVAPPGRFVLSSDAVVNDSGLPDVVATEAKQVQVEYLPESTL
                     VYLLGSRYCETELLSDIAWKQFGNGPTGWARVQAICDFVHRHIEFGYPHARNTRTAWE
                     AYQEGRGVCRDYAHLAVALCRCMNIPARYCTGYLGDMGTEPPYGTMDFAGWFEAYLGD
                     RWYTFDARNNTPRIGRILIARGRDACDVALSSTFGPNSLERFKVWADEVKVV"
     gene            1372..2094
                     /locus_tag="VLN25_01005"
     CDS             1372..2094
                     /locus_tag="VLN25_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HSQ09185.1"
                     /translation="MTYCVGVKLEEGLILASDSRTHAGVDNYAKFCKMTVFERAGDRV
                     LVLLSSGGLAATQAVINVLRQQAVAEPPHIWSASSMFDVANLVSDAMRAIERRDGPFL
                     EASDLKFNASFILGGQIAGEDMRLFRLYAEGNFIEAGTDTPFLQTGEAKYGKPILDLA
                     VTSSATLEDATKCVLVSYDSTMISNLSVGMPIDLLCYARDSLQVTMRRRFEEGDVYFD
                     ALTQQWLEGTRRVFHELPPLQW"
     gene            complement(2123..2848)
                     /locus_tag="VLN25_01010"
     CDS             complement(2123..2848)
                     /locus_tag="VLN25_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HSQ09186.1"
                     /translation="MTLARRNFLLITCEHGGREVPQEHLALFSGRDSLLASHRGWDAG
                     AFELARELAAAFGAPLYAANITRLLIDLNRSIGHRQLFSEVTRPLSRAQRLAIVDRYY
                     RPHRKMVESQVARHIAQGHRVIHVASHSFTPTLDGVLRQADVAWLYDPRRPGEAAFAQ
                     AWMLALAKLAPELRLRRNYPYQGRADGLTASLRRRHSDAEYVGIELEVNQRIVEQGGA
                     AWDRLKTMLVESMRNVLTAIAKR"
     gene            3278..3736
                     /locus_tag="VLN25_01015"
     CDS             3278..3736
                     /locus_tag="VLN25_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HSQ09187.1"
                     /translation="MNKFLLGRLLILSIAASFTVSTPYAAAPSVQEGRTAQDRRFIAG
                     GIGLDESEQMKSMARDFTLSITVAARSGAYLADSHVTIHDARGKVVLDTQLGAPYLLV
                     NLASGKYNIEATLQGKKQQQSVDIAEGKRAKIVFSFDVPVDRARENSPNQ"
     gene            complement(3838..4446)
                     /locus_tag="VLN25_01020"
     CDS             complement(3838..4446)
                     /locus_tag="VLN25_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09188.1"
                     /translation="MMPLGTVALFRDAVYYTIESSPLLLAAKFALTGHAGVLLGLLML
                     RGNPNSPLDIRYWSTTPYRLGPDRAVTYSLLPTSRHRSDKPETLGEDYLSDAMQVHLD
                     RHEASFDFCVQLQEPGMPIEDASQRWDEAKSPFLKVATLRLPMQRFRNDRRAQQAEAL
                     SFSPAHALAAHAPLGGLNRARGKIYTALSRFRHKRDKRAEQA"
BASE COUNT          852 a         1344 c         1425 g          840 t
ORIGIN      
        1 cacggcccag gaccgtacgt gatgggcgcc gatactctcg tcggcaatga cgtctacaac
       61 cgtgactcgc aagatctcgg tgacatcaag gagatcatgc ttgacatgcg taccggacga
      121 gtgagttacg ccgttttgtc gttcggcggt ttcctgggca tgggagagaa gctctttgcc
      181 gtgccctggg acgcacttac gcttgacacc gaaaacaagc gtttcgtgct cgacgtagac
      241 aagacgcggc tccaaggtgc tccgggcttt gacaagaatc attggcccga catggccgac
      301 gccacctggg aagagggcat ccacgcttac tacgggacca agccttatag agagcaaccg
      361 cgcgtctgag cgttgcgcct gcaaccggcg ctcgtcgttc gtcgcgaagg gggagcacaa
      421 cgttggcagg cgtagatgcg tgcgccttct ggcagactcc ttggcgcgca gaactctgaa
      481 tcggattaat ttggcgacga caaacatgca gattcgtgtc ggtttcgaaa tggagtacca
      541 gtgtcgcggc ccgaccccga tggtgctcgc gctcagtatc caccactcgc gggcgtccga
      601 cctggtacgc ccggatcttc tcgtcaccag ccctgcggta acggtaaccg cctaccggga
      661 ccttttcggc aactggtgta gccgcctcgt tgcgccgccc gggagattcg tgctgagcag
      721 cgatgccgtg gtcaacgaca gcggcctgcc cgatgtcgtg gcaaccgagg cgaagcaagt
      781 tcaggtcgag tatctgccag agagcacgct ggtgtacctg ctcggtagcc gctactgtga
      841 gacggaactg ctgtccgaca tcgcatggaa gcagttcggc aatggcccga cgggctgggc
      901 gcgcgtgcag gcgatctgcg acttcgttca ccggcatatc gagttcggtt acccccatgc
      961 gcgcaacaca cgcactgcgt gggaggccta ccaggaaggg cgtggggtat gccgggacta
     1021 cgcgcacctg gcggtcgcgt tgtgccgctg catgaacatt cctgcgcgct attgcaccgg
     1081 gtacctcggc gacatgggca cggaaccgcc gtacgggacc atggactttg caggctggtt
     1141 cgaggcctac ctgggcgacc gttggtacac cttcgacgcg cgcaacaaca cgccacgcat
     1201 cggccgcatc ctcatcgcgc gcggtcgcga cgcctgcgac gtagcgctct ccagcacttt
     1261 tgggccgaac tcgctggagc ggttcaaagt ctgggccgac gaagtgaagg tggtctgagt
     1321 gcggcgtgtt ttctgcgatc tcgtggggcg cagaggggtc aggcgcgctg catgacttac
     1381 tgtgtcggtg taaagctcga agaaggcctg atcctggcca gcgactcgcg cacgcatgcg
     1441 ggcgtggata actacgccaa gttctgcaag atgacggtgt tcgagcgcgc cggcgaccgg
     1501 gtacttgtgt tgctcagttc ggggggactg gctgccacgc aggcggtgat caatgtcctg
     1561 cgtcagcagg ccgtggccga gccgccgcac atctggagcg ccagttcgat gttcgacgtg
     1621 gccaatctcg tatcggatgc catgcgtgcc atcgagcgcc gcgacggccc gtttctggaa
     1681 gccagtgacc tgaagttcaa tgcgtcgttc atcctcggcg gccagatcgc cggcgaagac
     1741 atgcgtctgt tccggttgta tgccgaagga aattttatcg aagccggaac cgatacgcca
     1801 ttcctgcaga ccggtgaagc caagtacggc aagccgatcc tcgacctcgc ggtgacctcg
     1861 agcgcgacct tggaagacgc taccaagtgc gtgctggtgt cttatgactc gacgatgatc
     1921 agcaacctct cggtgggcat gccgatcgac ctgctgtgct acgcgcggga cagcctgcag
     1981 gtgacgatgc ggcgccggtt cgaggagggc gacgtctatt tcgacgcgct gacgcagcaa
     2041 tggctcgagg gcacgcgcag ggtctttcac gaactgccgc cactgcagtg gtaaatctcg
     2101 cgacgccgcg ggtgcaggag cctcagcgtt tcgcaatagc ggtcagcacg ttcctcatgg
     2161 attcgacgag catggtcttc agccgatccc aggcggcgcc gccctgttcg acaatgcgct
     2221 gattcacctc gagctcgatt ccgacgtatt ccgcatccga atggcgcctg cgcagcgacg
     2281 ccgtgagtcc gtcggcgcgc ccctggtacg ggtagtttct gcgaagacgc agctcgggcg
     2341 ccagctttgc gagcgccagc atccatgctt gcgcgaaggc cgcctcaccg ggacgccgcg
     2401 ggtcatagag ccaggcgaca tcggcttgcc tcagcacgcc gtcgagcgtt ggcgtgaaac
     2461 tgtgcgaggc gacgtggatc acccggtggc cctgcgcaat gtgtcgcgcg acctggcttt
     2521 cgaccatctt gcgatggggc cggtaataac ggtcgacgat tgcaaggcgc tgagctcgcg
     2581 aaagggggcg cgtgacctcc gagaacagtt gccgatgccc gatcgaccgg ttcagatcga
     2641 tcaatagccg cgttatgttg gcggcgtaca gcggcgcacc gaacgcagcg gccaattcac
     2701 gggcgagttc gaaagcaccc gcgtcccagc cgcggtggct cgccagcagt gaatcccggc
     2761 ccgagaagag cgcgagatgt tcctgcggga cctcacgtcc accgtgctca caggtgatga
     2821 ggagaaaatt cctgcgcgcg agtgtcaatg caactccagc ggccgcatgg ccacacgaat
     2881 gcatagcagg ttagctgcag aattgcctga aagcagaacc agtcgctccg ctggagcaaa
     2941 tgccgtgtca atgcgtatgc ctggtggcag gcagggaatc gccgtaccgt ggccagaacg
     3001 cccaccggta cgtgcgctgt cgaacagaca tcgcgcccgg caacgtctac aaattgaatc
     3061 ttggtggttc ttttccggta gccgaccgcg ccatagaagt cagcgcaagg cggaacgtga
     3121 tttgtcgcct aacgaaacga tgcgggtacg gctttcaaga cttccttaac aggagtcgta
     3181 ggcgcgcgct tgcgtacggg tcgaaccggc ccatcaatga cgagaaggcg aaccgggagc
     3241 gctgcttgct gcgcttccat gaccaacaga ggtgaccatg aacaaatttc tgctaggtag
     3301 attgctaatt ctgagtattg ctgcttcctt tacggtctcg acgccatatg ccgctgcccc
     3361 gtcggttcag gagggacgca cagcacagga ccgccgcttt atcgccggtg gcatcggact
     3421 tgacgaatcc gagcagatga aatcaatggc tcgcgacttc acgttgtcga tcactgtggc
     3481 ggcaagatcg ggggcgtatc tggccgattc gcacgtcacg atccacgatg cgcggggaaa
     3541 agtggtcctc gacacgcagc taggtgcacc ttacctgttg gtcaacctgg cctcgggaaa
     3601 gtacaacata gaggccacgc tccaagggaa gaaacagcag cagagtgtcg acatcgccga
     3661 aggcaagcgc gccaagatcg tgttcagctt cgacgtcccg gttgaccgag cgcgcgaaaa
     3721 ctcgccgaac cagtaatcag gtcgcgcctg gttagcctcg catctggtca gcgcgttggc
     3781 gccacgggcc accgggtttc ctcagcggcg tgcagcccgg ctgatcgaaa cggcgcttca
     3841 ggcctgttcg gcgcgcttgt ctcgcttgtg cctgaacctc gagagcgccg tgtaaatctt
     3901 gccgcgcgcg cggttcagcc cgcccagcgg agcgtgcgcc gccagggcat gcgcgggtga
     3961 gaacgacagc gcctcggctt gctgagcgcg cctatcgttg cgaaatctct gcatgggcaa
     4021 gcgcaaagtg gccactttga ggaaaggtga cttcgcctcg tcccagcgtt ggctggcgtc
     4081 ctcgattggc atgccgggtt cttgcagctg cacacaaaaa tcgaagctcg cttcatgccg
     4141 gtccagatgg acctgcattg cgtcgctcag gtagtcctcc cctaaggttt cgggcttatc
     4201 gctgcggtgt ctggacgtgg gcagcagcga gtacgtcacc gcgcggtcgg ggcccaagcg
     4261 gtagggcgtg gtgctccagt agcggatgtc cagcggcgag ttcggattcc cgcgcagcat
     4321 cagcaagccc agcagcaccc cggcgtggcc ggtcagcgcg aacttggcgg ccagcagcaa
     4381 gggcgagctt tcaatcgtgt agtagacagc atcgcgaaac agcgccactg tgcccagcgg
     4441 catcaccggt gtgtccagca g
//
LOCUS       DATIRT010000047         1725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1775807, whole genome shotgun sequence.
ACCESSION   DATIRT010000047 DATIRT010000000
VERSION     DATIRT010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1775807"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VLN25_01025"
     CDS             <1..414
                     /locus_tag="VLN25_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HSQ09189.1"
                     /translation="IDTERERISLGVKQLSGDPFTNFAAGHEKGSMVTGTVKSVDAKG
                     AVIDLGGDIEGFLRASEIAADRVEDARNHLKEGDAVNAMIINVDRKNRNINLSIKAKD
                     TADTSATMERMQAEAQTGTTNLGALLKAKLDTQTK"
     gene            471..788
                     /locus_tag="VLN25_01030"
     CDS             471..788
                     /locus_tag="VLN25_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HSQ09190.1"
                     /translation="MTKSELIARLAERNPRLVARDADEAVNTMLEAMTEALASGQRIE
                     IRGFGSFALNYRPPRVGRNPKSGDRVQVPAKYVPHFKAGKELRERVDGEEESASPGGA
                     NGG"
     gene            903..1241
                     /locus_tag="VLN25_01035"
     CDS             903..1241
                     /locus_tag="VLN25_01035"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HSQ09191.1"
                     /translation="MRLLAWMVKVGFFLLVLWFALKNTTPVPVRLTSAISWEHVPLIV
                     VMLACIAVGALLGALALALPIYRMRREMTNLRQQAPVVATAESAADRMASAARQSGAV
                     VEVAGEPERR"
     gene            1241..>1725
                     /locus_tag="VLN25_01040"
     CDS             1241..>1725
                     /locus_tag="VLN25_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HSQ09192.1"
                     /translation="MEFELWYLVALPLVFAAGWFGRGFESRARASDNAALPRSYFRGL
                     NLLLNDQHDKAIDAFIEVVKLDPETIELHHALGNLFRRRGEFDRAVRIHTHLLNRADL
                     PARERSQALAELGQDYLKGGLLDRAEDAFTRLLEDRDHRFDALRALLRIFQMEREWMK
                     A"
BASE COUNT          314 a          537 c          579 g          295 t
ORIGIN      
        1 atcgacaccg agcgcgaacg catttcgctg ggcgtcaagc agctgtcggg cgatccgttc
       61 accaatttcg ccgcgggcca cgagaagggg tcgatggtta caggcacggt caagtccgtc
      121 gatgccaagg gtgcggtgat cgaccttggc ggagacatcg agggcttcct acgtgcctcg
      181 gagatcgctg ctgatcgcgt cgaggacgcg cgcaatcacc tcaaggaagg tgatgccgtc
      241 aacgcgatga tcatcaatgt ggatcgcaag aaccgcaaca tcaacctgtc gatcaaggcg
      301 aaggacacgg ccgacacgtc ggcgacgatg gagcgcatgc aggccgaggc acagaccggg
      361 acgacgaatc tcggcgcgct gctgaaggcg aagctggata ctcagacgaa gtaatcgggc
      421 agacgaagta atcgggcaga cgaagcagcg ggagccgttc gttgaagacg atgacgaaat
      481 cggagttgat cgcgcgcctg gcggagcgca atccccgcct ggtcgcgcgc gacgccgatg
      541 aagccgtcaa tacgatgctg gaagcgatga ccgaggcact ggccagcggc cagcgcatcg
      601 agatccgcgg gttcggcagc tttgcgctga actaccggcc gccacgtgtc ggacgcaatc
      661 cgaagtcggg cgatcgcgtg caggtgcctg ccaagtacgt tccccatttc aaggccggca
      721 aggaactgcg cgagcgcgtc gacggcgagg aggaatccgc ctcgcccggc ggcgccaacg
      781 gcggctgaac atcgatactc ctgccgggcc gcgcatcgcg cggccctttt ttcgcccgca
      841 cggacgagac atcggttccc tacaatgccg gctttgctga tgcggtggta ccggaggcgt
      901 gtatgagact gctggcgtgg atggtcaagg tcggcttctt cctgctcgta ctctggtttg
      961 cactgaaaaa cacgacgccg gtgccggtgc gactgacctc ggccatcagc tgggagcacg
     1021 tcccgctcat cgtcgtgatg ctcgcctgca tcgccgtcgg tgcgcttctc ggagcgctgg
     1081 cactggccct gccgatttac cgcatgcggc gtgagatgac caacctgcgg cagcaggccc
     1141 cggtggtcgc gaccgcggag tccgcggcgg accggatggc ctcggcggcg cggcagtcgg
     1201 gcgcggtggt cgaagtggcc ggcgaaccgg agcgtcgttg atggagttcg agctctggta
     1261 tctcgtcgcg ttgccgctcg tcttcgccgc gggctggttt ggccgcggct tcgaatcgcg
     1321 cgcgcgcgcg tcggacaacg cggcgctgcc acgctcgtat ttccgcggcc tcaacctgtt
     1381 gttgaacgac cagcacgaca aggcgatcga cgccttcatc gaggtcgtca agctcgatcc
     1441 cgagaccatc gagctgcacc atgcgctggg caatctattc cgtcgccgcg gcgagtttga
     1501 tcgcgcggtc cgcatccata cgcacctgct caaccgcgcc gacctacccg cgcgcgaacg
     1561 ttcacaagcg cttgccgaac tgggacagga ctatctgaag ggcgggctgc tcgaccgtgc
     1621 cgaggacgcc ttcacgcggc tgctggagga ccgcgaccat cgcttcgatg ccctgcgtgc
     1681 gttgctgcgg atcttccaga tggagcgcga atggatgaag gcgat
//
LOCUS       DATIRT010000048         2876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1382844, whole genome shotgun sequence.
ACCESSION   DATIRT010000048 DATIRT010000000
VERSION     DATIRT010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382844"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="VLN25_01045"
     CDS             <1..961
                     /locus_tag="VLN25_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HSQ09193.1"
                     /translation="AQLVIGTHALIQEAVSFDRLGLAIVDEQHRFGVAQRLALRGADT
                     GPAPHMLMLSATPIPRTLAMSYLADLDVSVIDELPPGRKPVVTKLVSTRRRDEVIARI
                     RADVAAGRQAYWVCPLIDVSDAVEAAAATETYTRIAAMLPELRIGLLHGQLPAADKTA
                     VMQEFSARAIDVLVATTVVEVGVDVANATLMVIEHAERFGLAQLHQLRGRVGRGSEQS
                     YCILLFDEPLSEAARERLKIVYETSDGFEVARRDLMMRGPGEFLGARQSGVPLLRFAD
                     LERDALLVEQARAAAESLLAENAVSAREHVRRWYEGRYEFLAA"
     gene            1009..1956
                     /locus_tag="VLN25_01050"
     CDS             1009..1956
                     /locus_tag="VLN25_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HSQ09194.1"
                     /translation="MTLTELKYIVAVARERHFGRAAEACFVSQPTLSVAIKKLEEELG
                     TQIFERRTSEVTTTSAGERIVSQAQKVLDEAARIKEIARQGRDPLAGPLRLGVIYTIG
                     PYLLPGLVRQLLKDAPEMPLLLTENFTVNLMERLKNGEIDVAVMALPLPQIGLLLQAV
                     YDEPFIVAIPRNHAWSRRKSIPSEDLKKETMLLLGTGHCFRDQVLEVCPELSRFSSSA
                     EGIQKTFEGSSLETIRHMVGSGLGVTVLPATSVPEKPSRDSLLEYISFKPPAPDRRVV
                     LAWRKSFTRAPAIEALRQAILKCSLHGVTKLPDSPIETW"
     gene            2055..2564
                     /locus_tag="VLN25_01055"
     CDS             2055..2564
                     /locus_tag="VLN25_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HSQ09195.1"
                     /translation="MAKKRKAMAAAHGAPGIDIGIADKDRARIAEGLSRVLADTYSLY
                     LKTHSFHWNVEGPMFNTLHLMFMDQYTELWTALDAIAERIRSLGYPAPGTNSEFARLT
                     SIDETPGVPDAMEMVRLLVAGHEAVTRTTRAVFPAADKASDESTADLLTQRLQIHEKT
                     AWMLRSLLK"
BASE COUNT          530 a          920 c          942 g          484 t
ORIGIN      
        1 cgcgcagctc gtcatcggta cgcacgcgct gatccaggag gccgtcagct tcgaccgcct
       61 tggactggcg atcgtcgacg agcagcaccg gttcggggtc gcacagagac tggcgctgcg
      121 cggagccgac acaggcccgg ccccccacat gctgatgctc tcggccacgc cgattccacg
      181 cacgctggcg atgagttacc tcgcggacct cgacgtctcg gtcatcgacg agctgccgcc
      241 cgggcgaaaa cccgtggtga caaaactggt ctcgacgcgc cgccgcgatg aggtgatcgc
      301 ccgcatccgc gccgacgtag ccgcgggccg gcaggcgtac tgggtgtgtc cattgatcga
      361 tgtcagcgat gccgtcgaag cagccgcggc gacagagacc tacacgcgca tcgcggcaat
      421 gttgcccgaa ctgcgtatcg gcctgctcca tggacagttg cctgcggcgg acaagaccgc
      481 cgtgatgcag gaattcagcg cacgcgcgat cgacgtactg gtggcgacga ccgtcgtcga
      541 agtcggcgtc gatgtggcga acgcgacgct gatggtgatc gagcacgccg agcgcttcgg
      601 actcgcccag ctgcaccagt tgcgcggacg cgttggccgc ggaagcgagc agagctactg
      661 catcctgctg ttcgatgaac cgctgtcgga ggctgcgcgc gagcgcctga agatcgtcta
      721 cgagaccagt gacggattcg aagtcgctcg gcgcgacctt atgatgcggg gtccgggcga
      781 gtttctcgga gcgcggcagt ccggagtccc gctgctgcgc ttcgccgacc tggagcgcga
      841 tgccctgttg gtcgagcagg cgcgtgcggc ggccgaatcc ttgctcgccg agaacgccgt
      901 gtcggcgcgt gagcacgtgc ggcgctggta cgaggggcga tacgaatttc tcgcagcatg
      961 atgcgcagct ggacgcgcac gcgggctgcg tgtgcagcac attgacgcat gaccctgacc
     1021 gaactcaagt acatcgttgc agtcgcgcgc gaacgacatt tcggccgcgc ggccgaggcc
     1081 tgctttgtca gccagccgac cctttcggtg gcgatcaaga aactcgagga ggaactcgga
     1141 acgcagatct tcgaacgtcg caccagcgag gtgacgacca catcggccgg ggagcgcatc
     1201 gtcagccagg cgcagaaagt cctcgacgag gcggcccgca tcaaggaaat cgcgcgccag
     1261 ggccgcgatc cgctggccgg tccgttgcgc cttggcgtga tctacacgat cggaccgtac
     1321 ctgctgccgg gactggtgcg gcaactgctc aaggatgcgc ccgagatgcc gctgctgctg
     1381 accgagaact tcacggtcaa tctgatggag cggctgaaaa acggcgagat cgacgtggca
     1441 gtcatggcgt tgcccttgcc gcagatcggg ttgctgctgc aggccgtcta cgacgagccg
     1501 ttcatcgtcg cgattccacg gaatcatgcg tggtcccgcc gcaagagcat cccgagcgag
     1561 gacctgaaga aggagaccat gctgctgctc ggcaccggcc attgcttccg cgatcaggtg
     1621 cttgaagtgt gtccggaact gtcgcgattc tcctcgtcgg ccgagggcat ccagaagacc
     1681 ttcgagggat cctcgctcga gacgatccgc cacatggtgg gctcgggtct cggcgtcacg
     1741 gtgctgccag cgacgtcggt cccggaaaag ccgtcgcgcg actcgttgct cgagtacatt
     1801 tccttcaagc cgccggcacc cgatcggcgc gtggtgctgg cctggcgcaa atcattcacg
     1861 cgggcgcctg ccatcgaggc gctgcggcag gcaatcctca agtgctccct ccacggcgtg
     1921 accaagctgc cggattcccc catcgaaacc tggtgacccg ttccccctgc ggctatcgac
     1981 ggcgtagaaa ttgtcgattg gtcagcgcgg gacggaacgg ttaggatcaa ttcagaaatt
     2041 caaaggagaa cgcaatggca aagaaacgca aggcaatggc ggcggcccat ggcgcgccgg
     2101 gcatcgacat cggcatcgcc gacaaggacc gggcgaggat cgccgaagga ctgtcgcgcg
     2161 tgctggccga cacgtactcg ctgtatctca agacgcacag cttccactgg aacgtcgagg
     2221 gcccgatgtt caacacgttg cacctgatgt tcatggacca gtacacggaa ttgtggactg
     2281 ctctggatgc cattgccgag cgcatccgct cgctcggcta ccccgcgccg ggcaccaaca
     2341 gcgaattcgc gcgactgacg tcgatcgacg agacaccggg cgtgccggat gcgatggaaa
     2401 tggtgcggct gctggtcgcc ggccacgagg ccgtcacccg caccacgcgt gctgttttcc
     2461 cggcggccga caaggccagc gacgagtcga ccgccgactt gctgacgcaa cgactgcaga
     2521 tccatgaaaa gaccgcgtgg atgctgcgca gcctgctgaa gtagtagcgc gcggtctttc
     2581 gttggaggct aacttcggcg caggccgaag ttaagccgcg cgagcggcgg ccaaaaacaa
     2641 aaaacggcgt ggatgctccg cagtctgctg aagtaggctg ccgtccaggc tgggcaaccc
     2701 ccgcggaagc gggccattgc tagctggatc cgctgcgcag cttccagaca ccgtcgccgc
     2761 tgcggcaggc gaccttcgtg ccccacgact tcttgctgcc ttcgctggcc tcgagcttga
     2821 attcgcggca atcggtgccg acgcttttca tcggcagcag catgagattc gccccg
//
LOCUS       DATIRT010000049         3938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_202894, whole genome shotgun sequence.
ACCESSION   DATIRT010000049 DATIRT010000000
VERSION     DATIRT010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202894"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="VLN25_01060"
     CDS             <1..956
                     /locus_tag="VLN25_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HSQ09196.1"
                     /translation="AAAALTLAAGTPLAQTKLTLGHGAAPGNPRHEAAVAFANAVKQK
                     SGGKIEVQVAPSAQLGDDAAMITALRSGTLDMSANSQGAASVVVPEYAAFGMPFLFAD
                     PAAAFKLLDGPVGKELAEKSAAKNLVVLGYWDNGIRHVSNGKRAVLTPADMKGLKIRT
                     PPDAVTIDIMTALGAETQQIKFAELYVALQQGVVDGQENPLANVDASKIYEVQKFISL
                     TGHKFEMTPLLISKRTWDRLSEADRKIVQEAANEATAVQRKLSKEADDRLMVELPKKG
                     VKIDKVDQSQFAKATESVTTKWTSGPIGDYVKKVVAAARAK"
     gene            1028..1531
                     /locus_tag="VLN25_01065"
     CDS             1028..1531
                     /locus_tag="VLN25_01065"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HSQ09197.1"
                     /translation="MASVLGEKLSRVIASACAAILYLTFGAIFFILCSDVFLRYFAGS
                     SLRWASEIPELLFPWLVMAGVVLAAQHGAHIATTFLADAVHGKARRVIASVGHAAIIA
                     AYGLLAVVTAQAIPIVADERSPILGIPGSVTYICLFIGFVLIALVHLGELLHWVDERH
                     EQVTGAF"
     gene            1531..2790
                     /locus_tag="VLN25_01070"
     CDS             1531..2790
                     /locus_tag="VLN25_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HSQ09198.1"
                     /translation="MSAVALLIFALAAIAAIPIAHALVLAAIGGILTSDRVPLSLVVE
                     QMVSQVQSFPLIAIPFFILTGALMMGGRLGRTLIDLLTTLIGRFHGGPAQVGVLSSTL
                     FGGVSGSAVADASAIGSMLIPWQKKLGYPGGFSAATLASAATIDILIPPSIPLIIYAL
                     ASGASIGALFVAGILPGLMLCVGFMAVCYFVGRAKNFPREVRPISVGVVLREVGYASP
                     ALLLPVIIIIFLRGGVATPTEVSVISTLYAAVVSMVVYRDLTLQRVKAAVLESGLATG
                     VVLLVIMASSAMGWIITFEQLPVAFAEFAKNTLQDPWLIILAMNLIMLVAGMFIDLPA
                     AVLLLTPIFVPLAAMAGMDTVQLGVMMVVNLAIGLYTPPVGTTLFITSSIAKVKIGET
                     VRAMTPFYLVALLVLLLMSYVPALTIR"
     gene            2822..>3938
                     /locus_tag="VLN25_01075"
     CDS             2822..>3938
                     /locus_tag="VLN25_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HSQ09199.1"
                     /translation="MSTLEATKAPLQANADLQRLAHAIRFVSIDAIVRATEGHQGVPL
                     GMAEIATALFTRHMKFNPADPTWPDRDRFVLSNGHGSMLLFSLLYLTGYEQISIDQIK
                     TFRELGSHCEGHPEFDPASGIEVTTGPLGQGIANTFGMAVAEAYLNARFGSGLVDHYA
                     YAFVGDGCLQEGIGQEMISLAGHLRLGKLVLFWDDNRITDDGSTQLSISEDVAERFRV
                     ANWHVVEVDGHDLEAVSDAIAAAKKDPRPSMIACRTVIAKGIARLQGQRGGHSGRLHP
                     EDAAAARQSLDWPHPSFEVPEDVLKEWRAAGNSSDREHAAWTQRVAALPASERAEFER
                     VLRGELPGGWREVLLDYKRRAVIAAKPQGGIMISAEIN"
BASE COUNT          661 a         1268 c         1273 g          736 t
ORIGIN      
        1 tggccgctgc cgcgctcaca ctcgcggccg gcactccatt ggcccagacc aagctcacgc
       61 tcggccacgg cgccgccccg ggcaatccgc gccatgaagc cgcggtggcc ttcgccaacg
      121 ccgtcaagca gaagagcggc ggcaagatcg aggtccaggt cgcgccctcg gcacagctgg
      181 gtgacgacgc cgcgatgatc acggcgctgc gttccggcac gctcgacatg tcggccaaca
      241 gccagggcgc ggcgtcggtc gtcgtgccag agtacgccgc gttcggcatg ccgttcctgt
      301 ttgctgatcc ggccgccgcg ttcaagctgc tcgacggtcc ggtcggcaag gaactggccg
      361 agaagtcggc cgccaagaac ctggtcgttc tcggctactg ggacaacggc attcgccatg
      421 tctcgaacgg caagcgcgcc gtgctcacgc ccgccgacat gaagggcctg aagatccgca
      481 cgccgccgga cgccgtgacg atcgacatca tgacggcgct gggtgccgag acgcagcaga
      541 tcaagttcgc cgaactctac gtcgccttgc agcagggcgt ggtcgacggc caggagaacc
      601 cgctagccaa cgtcgatgcc tccaagatct acgaagtcca gaagttcatc tcactgactg
      661 gccacaagtt cgagatgacg ccactgttga tcagcaagcg aacctgggat cgcctgtccg
      721 aggccgaccg caagatcgtt caggaagctg ccaatgaagc gaccgccgtg cagcgcaagc
      781 tgtcgaagga ggccgacgac cgcctcatgg tcgaactgcc gaagaagggc gtgaagatcg
      841 acaaggtcga ccagtctcag tttgccaagg cgaccgagtc ggtgaccacc aagtggacga
      901 gtggcccgat tggcgactac gtcaagaagg ttgtcgccgc agcgcgcgcc aagtaagaac
      961 aagcgggaca cccgcgtgaa cggcgggccg gccacgggcc ggtccgcaac ttccaaagga
     1021 gacaaccatg gcttcggtgc ttggggaaaa gctgagtcgc gtcatcgcgt cggcctgcgc
     1081 tgcgatcctg tatctgacct tcggcgccat ctttttcatt ctctgttccg atgtgttcct
     1141 gcgttacttc gccggatcga gcctgcggtg ggcgtccgag attccggagc tgctgttccc
     1201 gtggctcgtg atggccggcg tcgtcctcgc cgcgcagcac ggcgcgcaca tcgcgacgac
     1261 gtttctcgct gacgcggtgc acggaaaggc gcggcgcgtc atcgcttccg tggggcatgc
     1321 ggcgatcatt gccgcctacg ggttgctggc tgtcgtcact gcgcaggcaa tcccgatcgt
     1381 cgcggacgaa cgttccccga ttcttggcat acccggatcc gtcacctaca tctgcctgtt
     1441 tatcggtttc gtgctgatcg cactggtcca cctcggcgag ttgctgcact gggtcgacga
     1501 acgtcacgag caagtcacgg gagccttctg atgagcgccg tcgccctact catattcgca
     1561 ctggccgcga ttgcggcgat tccgatcgcc cacgcgctgg tgctcgccgc gatcggcggc
     1621 atccttacct cggatcgggt cccgctgagt ctggtcgtcg agcagatggt gtcgcaggta
     1681 cagagctttc cgctgatcgc gattccattt ttcatcctga ccggcgcgct gatgatgggc
     1741 ggccgcctcg gcaggacgtt gatcgatctg ctgacgacgt tgattggccg atttcacggc
     1801 ggcccggcgc aggtgggcgt gctttcgtcg acgctgttcg gtggtgtctc gggctcggcc
     1861 gttgccgacg catccgcgat cggctcgatg ctgattccct ggcagaagaa gctgggctac
     1921 ccgggcggtt tctcggccgc cacgctggcc tccgcagcaa cgatcgacat cctgataccg
     1981 ccgtcgattc ccctgatcat ctatgcactc gcttccggcg cgtcgatcgg tgcgctgttt
     2041 gtagcgggca tcctgccggg cctgatgctg tgcgtcggct tcatggccgt gtgctacttc
     2101 gtagggcgcg ccaagaactt tccgcgggaa gtgcggccga tcagtgtggg agtcgtgctg
     2161 cgcgaagtcg ggtacgcgtc gccggcgctg ctgctgccgg tcatcatcat catcttcctt
     2221 cggggcggcg tcgccacgcc gaccgaggtc agcgtcatca gcacgctcta cgcggcagtg
     2281 gtgtcgatgg tggtttaccg tgacctgacg ttgcagcggg tcaaggcagc cgtgctcgag
     2341 tcgggactcg cgaccggcgt cgttctgctc gtcatcatgg cgtcgagcgc gatgggctgg
     2401 atcatcacgt tcgagcaatt gccggtggcg ttcgccgagt tcgcgaagaa cacgctgcag
     2461 gatccgtggc tgatcatcct ggcgatgaac ctgatcatgc tggtcgcggg catgttcatc
     2521 gacctgccgg ctgccgtgtt gttgttgacg ccgatcttcg tgccgctggc ggcgatggcg
     2581 ggcatggaca ccgtgcagct gggcgtgatg atggtcgtca acctggccat cggcctctac
     2641 acgccgccgg tgggcacgac gcttttcatc accagctcga tcgcgaaggt caagatcggc
     2701 gagaccgtac gggcgatgac gcctttctat ctggtcgcgc tgctcgtgct ccttttgatg
     2761 agctacgtgc ccgcgctgac gattcgctag gccggacttt cgaacgggaa ccacccatcc
     2821 catgagcaca ctcgaagcaa ccaaggcgcc cctgcaggcc aatgccgatc tgcagcgtct
     2881 cgcccatgcg atccgcttcg tctcgatcga cgcgatcgta cgcgcgacag agggccacca
     2941 gggcgttccg cttggcatgg cggagatcgc caccgcgctc ttcacccggc acatgaagtt
     3001 caatccggcc gaccccacgt ggccagaccg cgaccgcttc gtgctgtcca atgggcacgg
     3061 atcaatgctg ttgttttcac tgctgtacct gaccggctac gagcagatct cgatcgacca
     3121 gatcaagacc ttccgcgagc tcggttcgca ctgcgaaggc catccggagt tcgatcccgc
     3181 cagcggcatc gaagtgacaa ccggcccgct cgggcagggc atcgccaaca cgttcggcat
     3241 ggcggtcgcg gaggcctacc tgaacgcccg ctttggctcc gggctcgtcg accactacgc
     3301 gtacgcgttc gtcggcgacg gctgcctgca ggagggcatc ggccaggaga tgatctcgct
     3361 ggcaggtcac ctgcggctgg gcaagctggt tctcttctgg gacgacaacc gcatcaccga
     3421 tgacggcagc acgcagcttt cgatcagcga ggacgttgcc gagcgcttcc gtgtggccaa
     3481 ctggcatgtg gtcgaggtcg acggccacga cctcgaagcc gtgtcggacg cgatcgcagc
     3541 ggcgaagaag gatccgaggc cgtcgatgat tgcctgccgc acggtgatcg cgaagggaat
     3601 cgcacgtctg cagggacagc gcggtggcca ctccgggcga ctgcatccgg aagacgctgc
     3661 agcggcccgg cagtcgctgg actggccgca tccgtctttt gaagtccccg aggatgtact
     3721 caaagagtgg cgagccgccg ggaacagcag cgatcgcgaa cacgctgcgt ggacccagcg
     3781 cgtggctgca ttgccggcct ctgagcgcgc tgaattcgag cgcgtgctgc gcggcgaact
     3841 gcccggcggc tggcgcgagg tcctgctcga ttacaagcgg cgcgccgtga tcgcggccaa
     3901 accgcagggc ggaatcatga tctctgcgga aatcaacg
//
LOCUS       DATIRT010000050        13614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_989690, whole genome shotgun sequence.
ACCESSION   DATIRT010000050 DATIRT010000000
VERSION     DATIRT010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13614
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989690"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VLN25_01080"
     CDS             complement(<1..210)
                     /locus_tag="VLN25_01080"
                     /inference="COORDINATES: protein motif:HMM:NF013383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HSQ09200.1"
                     /translation="MNIAVLGAGAWGTAIACALGTLHSVVLQARDSAQVGDMRAAHSN
                     RRYLAGITFPPALSVTDGFDAAVAHA"
     gene            complement(218..736)
                     /gene="secB"
                     /locus_tag="VLN25_01085"
     CDS             complement(218..736)
                     /gene="secB"
                     /locus_tag="VLN25_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HSQ09201.1"
                     /translation="MTDTSPATAAGAQPVFQVQRVYLKDASLEMPNAPTVFLENEAPK
                     VDVQLEVSDAPVIEALHEVVVRVTVTAKVKDKVLFLVEGKQAGIFEIRNIPAEQRPPI
                     LGIVCPGIVYPYLRSNIADLISRTGLPPIHLAEINFEAFYQQRLAALAQQATGQPTAA
                     PLPANGNGAANA"
     gene            complement(816..1073)
                     /gene="grxC"
                     /locus_tag="VLN25_01090"
     CDS             complement(816..1073)
                     /gene="grxC"
                     /locus_tag="VLN25_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HSQ09202.1"
                     /translation="MSQVLMYSSGVCPYCQRAEALLKARGVTVIEKVRVDLEPARRDE
                     MVERTGRRTVPQIFIGQTHVGGFDDLSALDREGKLTPLLSQ"
     gene            complement(1090..1494)
                     /locus_tag="VLN25_01095"
     CDS             complement(1090..1494)
                     /locus_tag="VLN25_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HSQ09203.1"
                     /translation="MQFFIDNLLLIAVAFVSGAMLVWPFVRARASGPALSTLQATQLI
                     NSKNPQIVDLRSAEDFAKGTLPNARNVPAAAMSARLTELKKEKPVLLVCATGTSAGRT
                     AALLRSQGFSDVFVLGGGVAAWREAGLPLRSS"
     gene            1778..2533
                     /gene="gpmA"
                     /locus_tag="VLN25_01100"
     CDS             1778..2533
                     /gene="gpmA"
                     /locus_tag="VLN25_01100"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HSQ09204.1"
                     /translation="MFKLVLLRHGESQWNLENRFTGWTDVDLTENGRQEARTAGELLL
                     REGYGFDIAYTSVLRRAIRTLWIGLDALDLMWLPVVHSWRLNERHYGALQGLNKSETA
                     ARFGEQQVLTWRRAYAVAPDPLTPEDPRWAGREPRYRNLAPADIPATECLRDTVARVL
                     PFWKDSIAPAIQRGRRVIVVAHGNSLRALIKYLDNISDDDIVGLNIPTARPLVYELDA
                     DLRPLHRYYLGDADEIARATAAVAAQGQARSGA"
     gene            2605..3939
                     /locus_tag="VLN25_01105"
     CDS             2605..3939
                     /locus_tag="VLN25_01105"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HSQ09205.1"
                     /translation="MLGAGQARTPGVPPSGKRAAPPTTTKPAPKSQAPAAATPQRQQS
                     LQREQRELQSELAKLKRQLAASEASRSEASDALASSDIAISRANRRLRELSTARNRVE
                     QQIAALAQREQDVAVRQGERQSQLDQLLRRQQRLSLRDPLHLLLEGESPGDLGREQVY
                     LDYLTRTTDASVSQLQSRRAELAVLQERSQEKKEELAKIAEEEDKNRLTLEQEQARRK
                     RTLEQLSKQIIGQRQSIARLERDEQRLGTLIDRISKVLAEQSRREAERTRHLAEREAK
                     AKVAARGATAPKDPKPERAAARTPSDLPPSSSNFGQQKGKLRLPVDGAVSARFGAPRR
                     GDGGSTGPSWKGVFLQAPAGVDVKAVGDGQVVFADWLRGFGNLLVIDHGEGYLSIYGN
                     NEALLRNVGDRVAVGEVVASVGNTGGNEAPGLYFELRFQGRPFDPLTWVAAR"
     gene            3993..5441
                     /locus_tag="VLN25_01110"
     CDS             3993..5441
                     /locus_tag="VLN25_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HSQ09206.1"
                     /translation="MAGRLRSVGLISTGAIAGVLISLGISAYAFRDTRGQIPLEEIRQ
                     FTEVFGAVKQNYVEPVDDKKLMQEAISGMLSGLDPHSAFLDADAFKDMQSSTQGEFGG
                     LGIEVGAEDGIIKVITPIDDTPAARAGVKAGDLIIKIDDKVTRGMTLPEAVKLMRGKP
                     RTSITLSIARKGEDKPLEFKLMRDVIRVQSVRTKMIEPGYGFVRISQFQERTVDDLVK
                     QINELYKQAPLKGLVLDLRNDPGGLLHSAVGVSAAFLPQRALVVSTDGRNEDARRRFV
                     ASPEDYLRGRGEDVLQKLPTQIKSVPMIVLVNGASASASEIVAGALQDHKRATVIGTQ
                     TFGKGSVQTIIPLRLEGEGPAAIKLTTARYFTPSGRSIQAKGIVPDLAVEDTPEGNFS
                     GFNVREADLMRHLETNKTDDAARRVATPPGAAVQPAAPADSAPASKAAPSRRYEFGSS
                     EDFQLQQAMNHLKGKPVDVAKPKAENVAKAPR"
     gene            5500..6246
                     /gene="moeB"
                     /locus_tag="VLN25_01115"
     CDS             5500..6246
                     /gene="moeB"
                     /locus_tag="VLN25_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HSQ09207.1"
                     /translation="MNDEQLLRYSRHILLNEIGVEGQERLRAAHVLVIGAGGLGSPAA
                     LYLASAGVGTLTLVDGDRVDLTNLQRQILHTTGRIGMPKAESGRVALAAVNPEVNLVP
                     VEQHVDADRLQDLVARATVVLDCSDNFATRHAVNRCCVAHRRPLVSGAAIRFDGQVAS
                     FDVRDDASPCYACLFPPGDGEDELCSIMGVFAPLTGIIGSVQAAEALKLAAGIGIPLV
                     GRLLMLEALSMQWHSVRVPRDPDCPVCARR"
     gene            6251..7126
                     /locus_tag="VLN25_01120"
     CDS             6251..7126
                     /locus_tag="VLN25_01120"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HSQ09208.1"
                     /translation="MSGRHVALLFLLSAIWGGAFSLIRNAVPALGPIGLSAIRLVVAA
                     LLMLGYLRATGDRVHWRRDWRAIAVIGIFGAALPFPLFSFGATRLSAGVLAIINATVP
                     LWGAVLARVWLGDRMSPVAMLGIALGIGGVAALMGGGTVPPDAASRWAFAAGLAASLC
                     FAVAGVATKALGARVPAAALGAGVLVVGAAFNVPLAVVFPPNEVTVFALVNAVALAVL
                     ASALATGLYLRLIQEIGPTRSMTVTFLIPVWGIFWSALLLGEPITMAMIAACAVILAG
                     TALVVVGQRAAVASA"
     gene            complement(7129..7728)
                     /gene="slmA"
                     /locus_tag="VLN25_01125"
     CDS             complement(7129..7728)
                     /gene="slmA"
                     /locus_tag="VLN25_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HSQ09209.1"
                     /translation="MESASPITGPRKPGARKLQILQTLATMLEDPKGEKVTTAALAAR
                     LGVSEAALYRHFASKAQMFEGLIEFIETTIFGLINEISARETSGLKQVRSIVAMLLEF
                     AQTNKGMTRVLIGDALVNENERLQERMNQLIERIEASLKQSFRVAATEREVAENVDAS
                     ARAGLVVAFVIGRWHRFAKSGFRKFPGDALETQMPILVA"
     gene            complement(7896..9749)
                     /gene="sppA"
                     /locus_tag="VLN25_01130"
     CDS             complement(7896..9749)
                     /gene="sppA"
                     /locus_tag="VLN25_01130"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HSQ09210.1"
                     /translation="MARPGLIRRFFGGLWTAVDFSRRLVINLLFLLIVGLVLFVWLSS
                     DKPPRLSPDTALVLNIQGDVVEEYTVGAQEAAISEALGRDRFETRVRDILAALDGAAR
                     DPQITRVVLVLDEMGSVGLATLRELGAAVERVRGAGKPVVAWSESYTQGQYFLAAHAD
                     EVFLHPSGAVLIRGIGGVRGYYKDLLDKLGVKVNTFQAGRYKSFGEPFTRTGPTPEAQ
                     EAEAFLLNGLWSTWLADVERLRKLKAGSVMAAINDLPQRLLATQGDIAQLALNEKLID
                     GLKRRDEFRALMLERGAPRSRDDDETFRQISMYSYLRYVDTPRTGAAVAVVVAQGEIV
                     DGDDRPGTIGARAASELIQRARQDDDIKALVVRIDSPGGSASASELIREQLELTRKAG
                     KPVVVSMGDVAASGGYWIAMGGDEVIADAATITGSIGVFALIPTFTGTMEKLGVNTAG
                     AATTWIAESTDLTRPLDKRLAQVIEAGIGHTYSEFLDVVANNRQSTPEKINEVAQGRV
                     WSGAQARERGLVDSVGGLDLAVKSAARRAGLGDGFRVEYLEHEPRGLDRYLALFFGQL
                     GATLRNDFGWDTLARALTGTVAPRAPLELRLLLEAKDQPMRALSYCFCEVR"
     gene            complement(9817..10569)
                     /locus_tag="VLN25_01135"
     CDS             complement(9817..10569)
                     /locus_tag="VLN25_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HSQ09211.1"
                     /translation="MNPGTGRRGRAPVTLSAMRKVRAPHKAAAGRRAAPVWLFDLDNT
                     LHDASHAIFGAIDRRMTDYVERELGVERGEANRLRTLYWRRYGATLLGLVRHHGIDPH
                     HFLRETHDFDVAELLRAERGLVRLFARLPGRKLLLTNAPLAYAGSVLRELSLHRHFRT
                     RYSIERMRVHGTYRPKPSRLMLRSMLARERIRPSAAVLVEDSVTNLKAARSLGMRTVL
                     ITRHGASAARRSPAAVGLRISSLHELARRFPR"
     gene            complement(10614..11501)
                     /gene="argB"
                     /locus_tag="VLN25_01140"
     CDS             complement(10614..11501)
                     /gene="argB"
                     /locus_tag="VLN25_01140"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HSQ09212.1"
                     /translation="METAQIAPQLKADILSEALPYIRRFHGKTIVVKYGGNAMVDQNL
                     KEAFAHDVVLLKLVGMNPVVVHGGGPQIDEALERVGKKSTFIQGMRVTDAETMEVVEW
                     VLAGEVQGELVMLINAFGGQAVGLTGKDGGMIRARRLLIQDRNDPTKRHDIGQVGEIE
                     AINPAVVKALQDDAFIPVISPIGFGEDGLSYNINADVVAGKIAEILKAEKLVMLTNTP
                     GVLDKEGTLLTGLSARQIDELFADGTISGGMLPKISSALEAAKSGVNAVHIIDGRVDH
                     CLLLEILTDQGVGTMIKSH"
     gene            11750..11953
                     /locus_tag="VLN25_01145"
     CDS             11750..11953
                     /locus_tag="VLN25_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HSQ09213.1"
                     /translation="MLQTFRIDGMHCGGCVARVTRALKPLADDVEVTLDPPLAKLDVP
                     SALALADVQAAVAAVGDYSVTPA"
     gene            complement(12005..12460)
                     /locus_tag="VLN25_01150"
     CDS             complement(12005..12460)
                     /locus_tag="VLN25_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HSQ09214.1"
                     /translation="MIRFIVSAVLVVASGTAAAQVQVKDAWARPALQGQTATGAFMSL
                     MSSDGARLVGVSSPVAGVVEIHEMVMEGSVMKMRAIPGLDLPPGRSVELKPGGYHVML
                     MDLKRPLKAGERVPVELRIETRDKRLVTQPIEIEVATRAPAAAAGAHKH"
     gene            complement(12576..13610)
                     /locus_tag="VLN25_01155"
     CDS             complement(12576..13610)
                     /locus_tag="VLN25_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HSQ09215.1"
                     /translation="MVAGASLLVRGAAKLALSFGISPLVVGLTIVAFGTSAPEMAVSI
                     GAALEGKTDIAVGNVVGSNIFNVLFILGISSMIAPLAVHQQLIRQEVPVMIGCSLLLV
                     AIAFDGRIGRLDAALLTGLLVVYTVFLIIQSRRQSQAVAEELAREVPRPNGWDRHWSV
                     QLGLVAAGLFLLVLGSDWLVTAAVAFARVMGVSDAVIGLTIVAAGTSMPEVAASVAAA
                     LKGERDIAVGNVIGSNTFNILGCLGLSGLLAADGLPVASSLIAFDLWVLLAVAFACFP
                     VFITGREIARWEGAVFFLYYCAYATFLILETTRHAALPRFSAAMQSFVLPLTIITLLV
                     VMYGHGRRRR"
BASE COUNT         2360 a         4498 c         4388 g         2368 t
ORIGIN      
        1 cgcatgggcc acggccgcat cgaagccgtc ggtcaccgag agtgcaggcg ggaacgtgat
       61 gccggcgaga tagcgacggt tgctgtgcgc ggcgcgcatg tcgcccacct gcgccgagtc
      121 gcgcgcctgg agcacgacgg agtgcagtgt tccgagcgca caagcgatcg ccgtgcccca
      181 cgcgcctgcg ccgagcaccg cgatgttcat catgccctca ggcgttggcg gcgccgttgc
      241 cgttcgcggg gagcggtgca gcggtcggct ggcccgtcgc ctgctgtgcc agggcggcga
      301 gccgctgctg atagaacgcc tcgaagttga tctcggccag atgaatcggc ggcaagccgg
      361 tgcggctgat cagatcggcg atgttcgagc gtaaataggg gtagacgatg ccggggcaga
      421 cgatgccgag tattggtggc cgctgctcag cgggaatgtt gcgtatttcg aagatgcctg
      481 cctgcttgcc ttcgaccagg aacaaaactt tgtccttcac tttggcagtc accgtcacgc
      541 ggacgacgac ttcgtgcagc gcttcgatca ccggcgcgtc cgacacttcg agctggacat
      601 cgaccttggg cgcttcgttt tccaggaaga cggtcggcgc attgggcatt tcgagcgagg
      661 cgtccttcag gtagacgcgt tgcacctgga atacgggctg tgcgccagcc gctgtcgcgg
      721 gagaggtatc ggtcatggat cttcctggca aatcctcggc ggcggcgcca cctcggcgat
      781 gggttgaaat tcttaactct tgaagcggtc ccaggctact gggacagcag tggcgtcaac
      841 ttgccctcgc ggtccagcgc tgacagatcg tcgaatccgc cgacatgggt ttggccgatg
      901 aaaatctggg gcacggtgcg ccggcccgtg cgctcgacca tctcatcgcg ccgcgccggc
      961 tcgaggtcga cgcggacctt ctcgatcaca gtcacgcccc gggccttcag caacgcctca
     1021 gcacgctggc agtacgggca cacgcccgag ctgtacatca ggacttggct catgcatcac
     1081 tccgttgttt catgagctgc gcagaggcag tccagcctca cgccacgctg cgacgcctcc
     1141 gccgagcacg aacacgtcac tgaacccctg gctgcgcagc aatgccgcgg tccggcccgc
     1201 gctcgtgccg gtcgcgcata ccagcagcac gggcttttcc ttcttcagct ccgtcaggcg
     1261 ggcactcatc gctgcagccg gcacattgcg cgcattcggc aaggtcccct tcgcaaaatc
     1321 ctcggcactt ctaagatcga cgatctgggg attcttcgaa ttgatcaact gcgtcgcctg
     1381 cagagtggac agcgccgggc cgctggcccg cgcgcgcaca aacggccaga cgagcatcgc
     1441 gccactgacg aatgccacgg cgatcagaag gagattgtcg atgaagaatt gcacggtgcg
     1501 gccgatggga tgagtgcagg gaccattgcc ccgcaattgc tagtacgccc tgcctttact
     1561 gcctgccttt actgtccgcc ttgcttaccc gccactacgc cttgctcgca tgccagctcg
     1621 cgatttcagt gttgcgcccg ctctgactcg ggcggcaccc ccgatcactg ctcggatgac
     1681 tgctgagctt cagcgcgccg gcgccagcga aaagcgtcgg attatagaat aggtgtcccg
     1741 atgaagcgcc gcccacgcct ccctccccgt tccagtcatg ttcaaactcg tgctgctgcg
     1801 tcacggcgaa agccagtgga acctcgagaa ccgcttcacc ggctggaccg atgtcgacct
     1861 gaccgaaaat gggcgacagg aggcgcgcac agcgggcgag ttgctgctgc gcgaaggcta
     1921 cgggttcgat atcgcctaca cctcggttct ccggcgtgcg atccgcaccc tctggatcgg
     1981 actggatgcg ttggatctga tgtggctgcc ggtcgttcac agctggcggc tgaacgagcg
     2041 ccactacggc gcgctgcagg gcttgaacaa gtcagagacc gcggcccgtt ttggcgagca
     2101 gcaggtcctg acatggcggc gcgcctatgc cgttgctccg gatccactga cccccgagga
     2161 cccgcgctgg gctgggcgcg agccccgcta ccggaacctg gcaccggcgg acatccccgc
     2221 gaccgagtgc ctgcgcgaca ctgtcgctcg cgtccttccg ttctggaagg attcgattgc
     2281 gccagcgata cagcgcggtc gccgcgtcat tgtggtcgcg cacggcaatt ccctgcgcgc
     2341 cctgatcaag taccttgaca acatctcaga cgacgacatc gtcgggctca atatcccgac
     2401 cgcacgcccc ctcgtctatg aactggatgc ggaccttcga ccgctgcacc gttattacct
     2461 cggcgacgcg gacgagattg cgcgcgccac ggctgcggtc gccgcgcagg gccaggcgcg
     2521 atcgggcgct tgagcgcact gcggggtcgg cagctcgcgc tgcgcctgct ggccgcgctg
     2581 ctggccgcgc tgctggccgt gctgatgctc ggcgcgggcc aggcacgcac gcccggcgtg
     2641 ccacccagcg ggaagcgggc cgccccgcca acaacgacca agccggcgcc gaaatcccaa
     2701 gctcccgcgg cggctacacc ccaacgacag cagtcccttc aaagagaaca gcgcgaattg
     2761 cagtcggaac tcgcgaagct gaagcgacaa ctcgccgcca gcgaggcgtc gcgctctgag
     2821 gcctccgacg ctcttgccag ttcggacatt gccatctcga gggcaaaccg acgcttgcgc
     2881 gaactgtcga ccgcccgcaa tcgggtcgag cagcagatcg ctgcgctcgc gcagcgggag
     2941 caggacgtgg cggtccggca gggcgaacgc cagagccagc tcgatcagtt gctgcggcgg
     3001 cagcagaggc tgtctttgcg cgaccctctg catttgttgc tcgagggcga gtctccgggc
     3061 gatctgggcc gcgagcaggt ctacctcgac tatctcaccc gcactaccga cgccagtgtc
     3121 agccagcttc agagtcgccg cgcggagctg gctgtgcttc aggaacgatc gcaggagaag
     3181 aaggaagaac tggcaaaaat cgccgaagaa gaggacaaaa atcgactgac tttggagcag
     3241 gaacaggccc gccgaaagcg aaccctggag cagttatcca aacagatcat cggtcagcgc
     3301 cagtcgatcg cccggcttga gcgcgacgag cagcggctcg gcacgctcat tgatcggatc
     3361 tccaaggtcc tggcggaaca gtcgcgcagg gaagctgagc gaacgcgcca cctggcggaa
     3421 cgcgaagcga aggccaaagt cgcagcccgg ggggccacag caccgaaaga ccccaaaccc
     3481 gaacgggccg ccgcaagaac gccttccgat ttgccccctt cttcgtcgaa cttcgggcaa
     3541 cagaagggaa agttgcggct tcccgtggat ggggctgtgt cagctcgatt tggtgctccc
     3601 cgtcgcggag acggaggctc aacgggtccg tcctggaaag gcgtgtttct gcaggctccc
     3661 gcgggtgtcg acgtgaaggc ggttggcgat ggccaggtcg tgttcgccga ctggctgcga
     3721 gggtttggaa acctcctggt catcgaccac ggcgaagggt acttgtcgat ttatggcaat
     3781 aacgaggcgc tcctgaggaa cgttggcgac cgtgttgctg tcggagaggt agtcgcgtcc
     3841 gtcgggaata ccggtggcaa cgaggcccca ggcctatact tcgaactacg tttccagggc
     3901 cgtccatttg acccgcttac ctgggtagcg gcgcgctaag gtccgcttaa gactggcgac
     3961 ataccgtcgc gaattccgct tccggaggtg gcatggctgg caggctacgc agtgttggac
     4021 tgatcagtac cggcgcgatc gccggcgtac tgatcagcct tgggatctct gcgtacgcgt
     4081 tccgcgatac gcgcggccag atcccgctcg aagagatccg ccagttcact gaggtcttcg
     4141 gcgcggtcaa gcagaactac gtcgagccgg tcgacgacaa gaagctgatg caggaagcga
     4201 tctccgggat gctatccggg ctcgatccgc attcagcctt cctggatgcc gacgcattca
     4261 aggacatgca gtccagcacg caaggcgaat tcggcgggct gggcatcgag gtcggtgccg
     4321 aggacggcat catcaaggtc atcaccccga tcgatgacac tccggcggcc cgcgcgggtg
     4381 tcaaggccgg agatctcatc atcaagatcg acgacaaggt cacgcgcggc atgacgctgc
     4441 cggaggccgt gaagctgatg cgcggcaagc cgcgcacgtc gatcacgctg tcgattgcgc
     4501 gcaagggcga ggacaagcca ctcgagttca agttgatgcg cgacgtcatc cgggttcagt
     4561 cggtgcgcac caagatgatc gagccgggct acggcttcgt gcgaatttcg cagttccagg
     4621 agcgcacggt cgacgatctc gtcaagcaga tcaatgagct gtacaagcag gccccgctga
     4681 agggcctggt gctcgatctg cgcaacgacc ctggcggact gctgcattcc gcggtcggcg
     4741 tatcggccgc cttcctgccc cagcgcgcgc ttgtcgtttc caccgatggc cgcaacgagg
     4801 acgcgcggcg ccgcttcgtg gcgtctcctg aagactacct gcgaggccgt ggtgaggatg
     4861 tcctgcagaa gttgccgacc cagatcaaga gcgttccgat gatcgtgctc gtcaacggcg
     4921 cctcggcgtc ggcgtccgaa atcgtggccg gagcgctgca ggaccacaag cgtgcaaccg
     4981 tcatcggcac tcagactttt ggcaagggtt cggtccagac cattattcct ctgcgcctcg
     5041 agggtgaggg tccggcggcg atcaagctga ccacggcgcg ctatttcacg ccgagcggac
     5101 gctcgatcca ggcaaagggc atcgttcccg acctcgccgt cgaagacacc cccgaaggca
     5161 acttttccgg attcaacgta cgcgaagccg acctgatgcg gcatctggag accaacaaga
     5221 ccgatgatgc ggcaaggcgc gtggccacac ctccgggcgc cgctgtacaa cctgccgcgc
     5281 ctgcggatag cgcccctgcc agcaaggcag ctccgtcgcg gcgctacgag ttcggcagca
     5341 gcgaggactt ccagctgcag caggccatga atcacctgaa gggcaagccc gtcgacgtgg
     5401 ccaagcccaa ggcagagaac gtggcaaagg cgccgcgcta ggattcgcgc ttgcgactaa
     5461 cgggccgcgt tgcggcccgt tttcatttgg gccagacaag tgaacgacga acagctgctg
     5521 cgctattcgc ggcacattct tctcaacgaa atcggcgtag aggggcagga gcgcctgcgc
     5581 gccgctcatg tgcttgtcat cggcgcgggc ggcctgggat cacccgccgc actctatctt
     5641 gcatccgccg gggtcggcac gctgacactc gtcgatggcg atcgggtcga cctgaccaac
     5701 ctgcagcgac agatccttca cacgaccggc cgcatcggca tgccgaaggc cgagtcgggc
     5761 cgcgtggcgc tggctgccgt caacccggaa gtgaacctcg ttcccgtcga gcagcacgtc
     5821 gatgccgatc gactccagga tctggtcgcc cgggcaaccg tggttctgga ctgctcggac
     5881 aatttcgcga cgcgccatgc cgtcaaccgt tgctgcgtgg cccaccggcg accgctggtc
     5941 tcaggcgctg ccatccgttt cgacggacag gtcgcctcgt tcgatgtgcg cgatgacgcg
     6001 tcaccctgct atgcgtgtct gtttccaccc ggcgatggcg aagacgagtt gtgctccatc
     6061 atgggtgtat tcgcgccact gaccggcatc atcgggtcgg tccaggcggc cgaagcgcta
     6121 aagcttgccg ccgggatcgg cataccgctc gtgggccggc tgctgatgct ggaggcgttg
     6181 tcgatgcagt ggcactcggt acgcgtgccg cgggacccgg actgccctgt ctgcgcccgt
     6241 cgctgacgcg atgtcggggc ggcatgtggc actgctgttc ctgctctcgg ccatctgggg
     6301 tggggctttt tcgctgatcc gcaacgctgt accggcgctg ggcccgatcg gactgtcggc
     6361 gatacgtctg gtcgtggcgg ccctcctgat gctcggctac ctgcgggcca ccggcgaccg
     6421 cgtgcattgg cggcgtgact ggcgggccat cgccgtcatc ggcatcttcg gtgcggcgct
     6481 gccgtttccg ctgttctcct tcggcgccac ccggttgtcg gcgggagtgc tcgcgatcat
     6541 caatgcgacc gtgccgctgt ggggcgcggt cctcgcgcgc gtttggctcg gcgatcggat
     6601 gtcaccggtc gcgatgctcg gcatcgcgct cggtatcggc ggagttgccg cactgatggg
     6661 tggcggcacg gtacctcccg acgcggcgag tcgatgggcg ttcgcggcag gcctggctgc
     6721 atccctgtgc ttcgcagtgg ccggcgtggc caccaaggca ctgggagccc gcgttcccgc
     6781 ggcggcgctc ggagctggcg tactggtcgt cggtgcggcc ttcaacgtgc cgttggccgt
     6841 cgtgttcccg ccgaacgagg tcaccgtctt tgcgctcgtc aacgcggttg cgctggcggt
     6901 gctcgcctcg gcgctcgcca ccgggctcta tctgcggctc atccaggaaa tcggaccgac
     6961 tagatcgatg accgtgacgt ttctcattcc tgtctgggga atcttctgga gtgcgctgct
     7021 gctcggcgaa ccgatcacta tggcgatgat cgccgcttgt gcagtgatcc tggccggcac
     7081 tgcactggtc gtcgtcggtc agcgcgcggc cgtggcgtcg gcttaggtct aggcgaccaa
     7141 gatcggcatc tgcgtttcca gcgcgtcgcc cggaaacttg cggaagccgc tcttcgcaaa
     7201 gcggtgccag cggccgatca cgaaagcgac gaccaggccg gcgcgcgcgc ttgcgtcgac
     7261 gttttcggcc acttcgcgtt cggtcgcagc gacccgaaaa ctctgcttga gcgacgcttc
     7321 gatgcgctcg atcagctggt tcatgcgctc ctgcaggcgc tcgttctcgt tgacgagggc
     7381 atcgccaatc agcacgcgcg tcatgccctt gttggtctgt gcgaactcca gcagcatcgc
     7441 aacgatggag cgcacctgct tgaggcccga ggtctcgcgc gcgctgatct cgttgatgag
     7501 tccgaaaatg gtcgtctcga tgaactcgat cagaccttcg aacatctggg ctttgctggc
     7561 gaagtggcga taaagcgccg cttcggacac cccgaggcgc gccgcgagag ctgccgtggt
     7621 gaccttctcg cccttggggt cctcgagcat cgtcgcaagc gtctgcagga tctgcagctt
     7681 gcgcgccccg ggcttgcgcg gaccggtgat gggactggcg gactccatgg cggcggggag
     7741 cgagtgtggg agaacgagtc gcttccgggg aatgccccca gacgtgaatg catactatcc
     7801 cagaatcccg cagccgcgaa ccatcttcca gactaccggt agcttctcag cgcctcgaat
     7861 tgcggctgcc gcaggcgctt tactgcgcga tcggatcagc ggacctcgca aaagcagtac
     7921 gacagtgccc gcatcggctg atccttggcc tccagcaaga gccggagctc tagcggcgcg
     7981 cggggcgcca ccgtgccggt caacgcgcgc gccagggtgt cccaaccgaa gtcattccgc
     8041 agcgtcgcgc cgagctggcc gaagaaaagc gccagatagc ggtccagccc gcgcggctcg
     8101 tgttcgaggt attcgacccg gaaaccgtcg cccaacccgg cgcgacgcgc cgccgacttg
     8161 accgccaggt cgagccctcc gaccgagtcg accagtccgc gctccctggc ctgcgcgcct
     8221 gaccagactc gcccttgcgc gacctcgttg atcttctcgg gggtcgactg gcgattgttt
     8281 gcgacaacgt cgaggaactc gctgtacgtg tgcccgatcc cggcctcgat cacctgcgcc
     8341 aggcgcttgt cgagcggccg cgtcaagtcg gtcgactctg cgatccacgt ggtggccgca
     8401 ccggcagtat tgacgcctaa cttttccatc gtgccggtga aggtcgggat cagcgcaaac
     8461 acgccgatcg acccggtgat cgttgcggca tcagcgatga cctcgtcgcc gcccatcgcg
     8521 atccagtagc caccggacgc ggccacgtcg cccatcgaca cgacgaccgg cttgccggcc
     8581 ttgcgggtaa gttcgagctg ctcgcggatc aactccgacg ccgaagcact gccgcccggc
     8641 gaatcgatgc gcacgaccag cgccttgatg tcgtcgtcct ggcgtgcgcg ctggatcagt
     8701 tccgacgccg cgcgcgcgcc gatcgtgccc gggcgatcgt cgccgtcgac gatctcgccc
     8761 tgggcaacga ccacggcgac ggcagcaccg gtacgcggcg tgtccacgta acgcaagtag
     8821 gaatacatcg agatctgtcg aaacgtctcg tcgtcgtcgc ggctgcgcgg ggcaccccgc
     8881 tcgagcatca gcgcacgaaa ctcgtcgcga cgcttgaggc cgtcgatcag cttttcgttc
     8941 agcgcgagct gcgcaatgtc gccttgtgtt gccagcaacc gttgcggcag atcgttgatg
     9001 gccgccatga cgctgcccgc cttgagcttg cgcagacgct ccacgtccgc aagccaggtc
     9061 gaccacaggc cgttgagcag gaacgcctcg gcctcctgcg cctcgggtgt cggtccggtg
     9121 cgagtgaacg gttcgccaaa gctcttgtac cgccctgcct ggaaggtatt caccttcact
     9181 cccagcttgt cgagcaggtc cttgtaatag ccccgcaccc cgccgattcc gcggatcagc
     9241 actgcgccgg acggatgcag gaagacctca tcggcgtgcg cggccaggaa gtattgcccc
     9301 tgtgtgtagg actccgacca cgcaacgacc ggcttcccgg ctccgcgcac gcgttcgacg
     9361 gccgcaccca gttcgcgcag tgtcgccagc cccacgctgc ccatctcgtc gagcacgagc
     9421 acgacgcgcg tgatctgcgg gtcgcgcgct gcgccgtcca gcgcggccag tatgtcgcga
     9481 acccgcgttt cgaagcgatc gcgccccagc gcctcggaga tcgccgcctc ctgcgcgccg
     9541 acggtgtact cctcgacgac atcgccctgg atattgagga ccagggccgt gtcgggcgac
     9601 agccgcggag gcttgtccga tgaaagccag acgaacagca ccagtccgac aatcagaagg
     9661 aacagcaggt tgatgacgag acgacgcgaa aagtcgactg ccgtccacag gcctccgaaa
     9721 aatctgcgta tcagtccggg ccgggccata cgggctccac gtgcaatgca ggggtaggga
     9781 cgcggagctt accgaatcac tccgcggcac cccgggtcag cgtgggaacc gccgcgccag
     9841 ttcgtgcagg gacgagattc gcaggccgac agcggccggc gacctgcggg ccgctgaggc
     9901 gccgtggcgc gtgatcagca cggtcctcat gccgagcgac cgggccgctt tcaggtttgt
     9961 gacggagtct tcgaccagta ccgccgccga tggccggatg cgctcgcgcg ccagcatcga
    10021 gcgcagcatc aaccgcgacg gcttcggccg gtaagtcccg tgcacgcgca tgcgttcgat
    10081 cgagtagcgc gttcggaagt gacggtgcag ggacagctcc cgcaggacag acccggcgta
    10141 ggcgagcggc gcattcgtca acagcagctt gcggccaggg aggcgggcaa agagccgtac
    10201 cagcccgcgt tcggcacgca gcagctcggc cacatcgaag tcatgcgtct cgcgcaggaa
    10261 gtggtgcggg tcgatgccgt ggtgccgcac caggcccagt agcgtggcgc cgtagcggcg
    10321 ccagtaaagc gtgcgcagcc gattggcctc gccccgctcg actccgagct cgcgctcgac
    10381 gtaatcggtc atgcgacgat cgatcgcgcc gaagatcgcg tgcgaggcat cgtgcagcgt
    10441 gttgtcgaga tcgaacaacc acaccggcgc agcgcgacgc ccggccgccg ccttgtgcgg
    10501 tgcccgcacc ttccgcatag ccgacagcgt gaccggtgct cggccgcgac gccccgtacc
    10561 gggattcacg cgcggcgcgc gcaagcgcta cggcacgaat ggcgtggcat cgctcagtgg
    10621 ctcttgatca tcgtgcccac accctggtca gtcaggatct cgagcagcag acagtgatcg
    10681 acacggccgt cgatgatgtg cacggcattg acgccactct tggcagcctc gagggccgat
    10741 gagatcttgg gcagcatgcc gccggagatc gtgccgtcgg caaacagttc gtcgatctgg
    10801 cgtgccgaca gccccgtcag aagggtgcct tccttgtcga gcactccggg cgtgttggtc
    10861 agcatcacca gtttttcggc cttgaggatc tccgcgatct tgccggcgac aacatcggca
    10921 ttgatgttgt aggacagacc atcctcgccg aagccgatcg gggagatcac cggaatgaag
    10981 gcatcatcct gcagcgcctt gacgacggcg gggttgatcg cttcgatctc tccgacctgg
    11041 ccgatgtcat gacgcttagt cggatcgttc ctgtcctgaa tcagcagccg ccgtgcgcgg
    11101 atcatgcccc cgtccttccc ggtgagaccg accgcctggc cgccgaaggc attgatcagc
    11161 atcaccagtt cgccctggac ctcgccggcc aaaacccact cgaccacttc catggtttcg
    11221 gcatcggtga cgcgcatgcc ctggatgaag gtcgatttct tgcccacgcg ttcgagcgcc
    11281 tcgtcgatct gcggaccgcc gccgtgcacg accactggat tcatgccgac cagcttcagc
    11341 agcaccacgt cgtgcgcaaa ggcctccttg aggttctggt cgaccatcgc gttgccgcca
    11401 tatttcacga cgatcgtctt gccgtgaaac cggcggatgt acggcagcgc ctcggagagg
    11461 atgtcggcct tcagttgcgg agcgatctgc gcggtttcca tgactgtccg ttggtgtcct
    11521 ggcgacgcgc cagtgtgagg ccgcagcggt tcaggacgcg ccattgcgtg ctccctcccg
    11581 tgctgggcgc ggatgttata ccgacacgat cgaggcgtac ggcacgctgc gccgatcgac
    11641 agcgaactga accggcgtac ctcttgccgg agcagttgag gggttgcgcc gaaactccga
    11701 tggcaggggt aggattcatg ctccggggct tcgacgcagg agagaaagga tgctgcagac
    11761 ctttcgcatc gatggcatgc actgcggcgg gtgtgtggcg cgggtgacgc gcgccctgaa
    11821 gcccctcgct gacgacgtcg aagtcacact tgatcctccg ctggcgaagc tcgacgttcc
    11881 gagcgctctg gcgctggcgg acgtccaggc cgccgttgcg gccgtcggcg attacagcgt
    11941 cacgccggcg tgatccccgg ttcccgccgc aagtggatcc tgggtgcgcc gaagcggatt
    12001 gatcctagtg cttgtgggcg cctgccgcgg cagccggggc acgtgtcgcg acttcgattt
    12061 cgatcggctg cgtcaccagc cgcttgtccc tggtctcgat ccgcagttcg accggcacac
    12121 gttcgcccgc cttcagcggg cgcttcaggt ccatcaacat gacgtgatag ccgccgggct
    12181 tgagttccac gctgcgcccc ggcggcaggt cgagcccggg aatcgcgcgc attttcatca
    12241 cgcttccttc catcaccatc tcgtggattt cgactacgcc ggctaccggg ctggagacgc
    12301 cgaccagacg cgccccgtcg ctcgacatca gtgacatgaa ggccccggtg gccgtctgcc
    12361 cctgcagcgc cggccgcgcc cacgcatcct tgacctgcac ctgcgcggct gccgtaccgc
    12421 tggccacgac gagtacggcc gagacgatga aacggatcat gtgaactccc gagcgaaggg
    12481 ccggattcta tcgatccggc cgttgctctg tttcgttcgg atctggcggc cgggttgccg
    12541 ggacgatcgc acccgcgcga gcaagcacag ggtcgtcatc tacgtcggcg accgtgccca
    12601 tacatcacga cgagcagcgt gatgatcgtg agcggcagca cgaacgactg catggccgct
    12661 gaaaagcgcg gcaacgcggc gtggcgagtc gtttccagga tcaggaacgt ggcgtaggcg
    12721 cagtagtaca ggaagaacac cgccccttcc cagcgtgcga tctcgcgtcc ggtgatgaac
    12781 accgggaagc aggcgaacgc gacggccaga aggacccaca ggtcgaaagc gatgagggac
    12841 gacgccacgg gcaacccgtc tgccgccagc agcccggaca atccgagaca gccgaggata
    12901 ttgaaggtgt tgctgccgat cacgttgcca acggcaatgt cgcgctcgcc cttgagtgcg
    12961 gcggcgaccg aggcagcgac ttccggcatc gatgtcccgg cggcgacgat agtcagccca
    13021 atgaccgcgt ccgatacgcc catgacgcgc gcgaatgcga ccgccgccgt gacgagccag
    13081 tccgacccca gcaccagcag gaacagaccg gccgcgacca gacccagctg cacgctccag
    13141 tgtcgatccc agccattcgg ccgcgggacc tcgcgcgcaa gttcttccgc caccgcctgc
    13201 gactggcgtc gtgactggat gatcagaaac accgtgtaga cgaccagcag tccggtcagc
    13261 agcgcggcat ccaggcgacc gatgcgaccg tcgaaggcga ttgcgaccag cagcagcgag
    13321 cagccgatca ttaccggcac ttcctgccgt atcagctgct ggtggacagc cagcggcgcg
    13381 atcatcgagg aaatgccgag gatgaagagc acgttgaaaa tgttgctgcc gacgacattg
    13441 cccaccgcaa tgtcggtctt gccctcgagt gccgcaccga tcgacaccgc catttccgga
    13501 gcgctggtgc cgaaggccac gatcgtcagc ccgaccacca gaggcgagat accgaaggac
    13561 agcgcgagct ttgcagcccc gcgcaccagc agtgaagcgc ccgcgaccag cgcg
//
LOCUS       DATIRT010000051         8271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1382901, whole genome shotgun sequence.
ACCESSION   DATIRT010000051 DATIRT010000000
VERSION     DATIRT010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8271
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382901"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..1170)
                     /locus_tag="VLN25_01160"
     CDS             complement(250..1170)
                     /locus_tag="VLN25_01160"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014749594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HSQ09216.1"
                     /translation="MNERIRGVLAPVVTPFATDLRPDAGRLSRHCRWLVTQGVGLAVL
                     GTNSEANSLSVDERIGLLDQLIDDGIPAHRMMPGTGCCAISDSIRLTAHAVRHGVAGC
                     LMLPPFYYKGVTDEGLYRNFASIIDGVSDARLRIYLYHIPPVAQVGISLGLIERLLRD
                     FPSVIAGIKDSSGDWNNTQAVLGQFAALGFDVFAGSESFLLANLRHGGVGCISATANV
                     NPAPIDRLYREWRSTGADALQGELDAVRAIFQKRPMIAALKASIAHWSKDDEWARVRP
                     PLVELSPSQTSDLIADLDACAFEMPGIAQP"
     gene            complement(1372..1572)
                     /locus_tag="VLN25_01165"
     CDS             complement(1372..1572)
                     /locus_tag="VLN25_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09217.1"
                     /translation="MTRFESSILPDSFALEAAARRHRSEGYGKVFDAAVDWVESRLHN
                     LTRGLDDFTTPAAVRLHRPSSH"
     gene            1723..2676
                     /locus_tag="VLN25_01170"
     CDS             1723..2676
                     /locus_tag="VLN25_01170"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HSQ09218.1"
                     /translation="MLAFVRAVECNGFTPAARDLGVTPSAVSKLVTRLERKLGVRLLQ
                     RSTRRLALTAEGEIYFERVQRIVGEIADLESDVMRFAEQPRGRVRISVGTAFATYALV
                     PALPEFVARYPEITLDLVISEYAIDLIEAGIDLAIRVGPLGDVNLAARRIGELERVIT
                     AAPDYLAKLGLPRKPEDLARHNCLTLTGIAGQSEWPFRGPSGVRMLPVKGSITVNNAE
                     TLYELALQGLGVIRLSDLIVGPAIRNGKLVPILVDSHQPEPLPVHAVYAAGRRRPPKV
                     DAVIQFLAEKFSSAPWRTASSAPLSAARGREPASKRRGTRR"
     gene            2828..3385
                     /locus_tag="VLN25_01175"
     CDS             2828..3385
                     /locus_tag="VLN25_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HSQ09219.1"
                     /translation="MATQYYTASSLDGFIATADDSLDWLFQLGDVNDTGYPDFIRDVG
                     ALAMGSATYQWMLRHVIRTGSSSQGTWPYQQPCWVFSSRELPAVDSADIRFVRGDVRP
                     VHEEMQAAAATKNIWLVGGGDLVGQFFDAGLLDEVIVQICSVTLGAGKPLLPRFIAFP
                     PLHLTSARAIGTGFAELRYKVPSSN"
     gene            3431..3937
                     /locus_tag="VLN25_01180"
     CDS             3431..3937
                     /locus_tag="VLN25_01180"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HSQ09220.1"
                     /translation="MNIFVSYSPADNDLVSAIVSLLTASNGAIVYCDADSIRTSGRWR
                     EELEKAISEANLVMVFWCHHSNTSYEVRKEFALALERGKDVLPLLLDGTPLPGKLADR
                     RTIDFRGRVEVIHETSPGQAEAGGRADRADADRQIASGLSRQYEELLVMGLAREIETE
                     LATRLGTR"
     gene            complement(3961..4488)
                     /locus_tag="VLN25_01185"
     CDS             complement(3961..4488)
                     /locus_tag="VLN25_01185"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HSQ09221.1"
                     /translation="MPAKLDFSKLTLMDTLDLASLIEVEAFERYTLFARQLGIGYSGG
                     AGSVFQSMADNERKHGDQLAERRRTLFGNEPPQVTLDDIFDVEAPDVGSTRHDMSEFK
                     AYQVALHSEKKAFAFYDRALRSVKQPDVKALFEELRDEEAEHVRMIEEIIAKLPPSAK
                     VDIEDEDDTSMRMGF"
     gene            4794..5618
                     /locus_tag="VLN25_01190"
     CDS             4794..5618
                     /locus_tag="VLN25_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09222.1"
                     /translation="MKERSNRGHPVATALLFALVSATTVCAEAQTRPDETRFRATIYG
                     YFPGLSGTTEFPSGAGGPSITVNAKDLLEDLKMAFMGTLEMRSGKWGGLVDWVYADVG
                     SQKAGTREFSIHGQQLPVGLTANLSLDVKTNILTLAGTYSFIESPSYSTGLVFGARML
                     DMDNTLDWSFVGTGPAGIARTGRSEVSATNWDAIIGARGRAHFGSTLAWFVPYYVDIG
                     TGNSKVTWQAMTGLGYSFGWGDAIVVWRYLDYEFDSGEPTQSLTFNGVAVGLSFRF"
     gene            5718..6518
                     /locus_tag="VLN25_01195"
     CDS             5718..6518
                     /locus_tag="VLN25_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09223.1"
                     /translation="MSESEVLHPLRPILKNWVWEHGRVGTRYLDCTDGEIKFEDGKKA
                     RYASESIFYVPLGGDPGPSSEAGPTIHEGALARFLKAAQLGTPEEAGSVADVQRAAQD
                     CVDLCLVCAYQDDAQRAFAHYALEPMFEDEIRVAVVADIRDKYLGLRAQLALYDFTVF
                     YGLPAPLLISEAPFIDWRVRGQPVQPFVTLPLGPYCLLVGTPSGKKSRAGPVLWKAAS
                     VMGPFKDHNRHIVERAHRWLVATSDDQLAAVQDRYAVPGPDPAEAAKG"
     gene            complement(6539..6694)
                     /locus_tag="VLN25_01200"
     CDS             complement(6539..6694)
                     /locus_tag="VLN25_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09224.1"
                     /translation="MSASSGEKRRRKRERRFIALQEAISTRSTMVAVLATFGFVLLQV
                     ARTLLQG"
     gene            7018..7281
                     /locus_tag="VLN25_01205"
     CDS             7018..7281
                     /locus_tag="VLN25_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09225.1"
                     /translation="MTDEKLDELEAVCSAKADMDGMVRIDPGDVLELVRDLRELRKHA
                     AGLTALEGAVGTMHRLLGDLCAYGTRSPDIDVVNRIAVQDPGR"
     gene            7447..7704
                     /locus_tag="VLN25_01210"
     CDS             7447..7704
                     /locus_tag="VLN25_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09226.1"
                     /translation="MTTSRIAATLAGTIALAVLGTLALLAGSGGKAVAQQSPPACTCA
                     PVTSVASIGTNLVHCQCGPATCVVSEHFTGQSKTYALQCAK"
     gene            7909..>8271
                     /locus_tag="VLN25_01215"
     CDS             7909..>8271
                     /locus_tag="VLN25_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09227.1"
                     /translation="MSAVAHTHHLGTGFWRLADPKVTLASMSSILLGAAAAVQSGTYA
                     FSWLILVVVGIFALEWAKNASGEIYDFASGADQAVAESDRSPFSGGKRVLVDGLLTER
                     ETWTLAIVGYALCIGAGII"
BASE COUNT         1583 a         2528 c         2628 g         1532 t
ORIGIN      
        1 cagcgcggtg cctgccgcag cgaataccac tgcgacgatg atcatcgtca gcctgttcat
       61 cgcgcctctc cgtcaatcag gtaccggtgg acttacacgg gacagtacat cgctcaacgc
      121 ctcaggagct gccctgcagc attggagcgc gccacagcgg caaaggttca tcgattcacg
      181 tacaacgcac gggtcggagg tcgaggcaag gacgtgtcgc gcccgtgacc aggggaaggg
      241 tgatgcggat cagggttgtg cgattcccgg catctcgaat gcacaggcgt cgagatctgc
      301 aatcaaatcg ctcgtctgcg acgggctgag ctcgacaagg ggcggtcgga cgcgagccca
      361 ctcgtcgtcc ttgctccagt gtgcgatgga tgccttgagc gccgcgatca tcgggcgttt
      421 ctggaaaatg gcgcgtacgg cgtcgagttc gccctgcaat gcatcggcgc cggtggatcg
      481 ccactcgcga tacagcctgt cgatcggcgc cggatttaca ttcgcggtgg cgctgatgca
      541 accgacgccg ccgtgacgca ggttcgccag aaggaacgac tcgctgcccg cgaagacatc
      601 gaaccccaac gcggcgaatt gcccgaggac ggcttgcgtg ttgttccagt cgcccgacga
      661 gtccttgatt cctgcaatca cactgggaaa gtccctgagc agacgctcga tcaggccaag
      721 gctgatgccg acctgcgcca ccggtgggat gtgatacaga tagatgcgca gcctcgcatc
      781 gctgacaccg tcaatgatgg aagcaaagtt gcgatacaga ccctcgtcgg tgacgccttt
      841 gtagtagaac ggcggcagca tcaggcaacc ggcgacgcca tgacgaaccg cgtgagcggt
      901 cagccggatc gaatcactga tcgcgcagca gccggtgccg ggcatcatgc ggtgcgccgg
      961 aatgccatcg tcgatcagct ggtcgagcag gccgatgcgc tcgtcgacgg acagcgagtt
     1021 cgcttccgag ttggtgccta acaccgccag gccaacgcct tgagtgacta accagcgaca
     1081 gtgccgcgac aaacgccctg cgtccggtcg gagatcggtt gcaaaaggcg tcaccacggg
     1141 cgcaaggacg cctcggatac gttcgttcat tgcactgctt ttggggtcgc tttcgctcgc
     1201 ggagagcact tcaagcccgc cgcaaggcag gcttgatcgg tagagacatc gtaatgccag
     1261 tgatgagctg ttgctggagt cgcgctggcg tcgcagatag atggataggt gcgcagctgg
     1321 cctcagaaac gcgggttaga cgctgcacca gccgtgggat gaaagcgaac ttcagtgcga
     1381 cgagggtcga tgcaggcgca cggcagccgg tgtcgtgaag tcgtcgagac cccgggtgag
     1441 gttgtgcagg cgactttcca cccagtccac cgctgcatca aataccttgc cgtagccttc
     1501 gctgcggtgc cggcgagcgg ccgcttcaag agcaaagctg tctggaagga ttgaggattc
     1561 aaagcgtgtc atggcgtcct cctgtgatgc gactttggcg tgactgcaga cgaacgctaa
     1621 ctcccatcgc atcagcatac aatccgtatt tccggcatcc catttgtgcc ataaaggcac
     1681 aaatatccga ctaaggaggt ccgtacggaa tccagcctgg ccatgctcgc tttcgtccgt
     1741 gccgtcgaat gcaatggatt cacaccggcc gcgcgtgacc tcggcgtgac gccctctgcg
     1801 gtgtcgaagc tggtcacgcg cctcgagcgc aagctcggtg tacgcctgct ccagcgctcg
     1861 acgcgccgac tggcgctgac agcagagggc gagatctact tcgagcgcgt gcagcggatc
     1921 gtcggcgaga tcgcagacct cgagtcggac gtcatgcgct tcgccgaaca gccgaggggt
     1981 cgcgttcgca tcagcgtcgg tacggccttc gcgacctatg cactggtccc ggcgctgccc
     2041 gaattcgttg cgcggtatcc ggagatcacg ctcgacctcg tcatcagcga atacgcgatc
     2101 gatctcatcg aggcgggcat cgatctggcg atacgtgtcg gcccgctggg ggacgtcaac
     2161 cttgccgcgc gccgcattgg cgaactcgag cgggtcatca ccgcagctcc ggactatctg
     2221 gcgaagctcg gtttaccgcg caagcccgag gatctggccc ggcacaactg cctgacgctc
     2281 accggtattg ctggccagag cgaatggccc ttccgggggc ccagtggagt gcggatgctg
     2341 ccggtcaagg gcagcatcac ggtcaacaat gccgagacgc tttacgaact tgcactgcag
     2401 ggtcttggcg ttattcggct atcggatctc atcgtcggtc cggcgatacg gaacggaaaa
     2461 ctggtgccca tcctggtcga ttcacaccag cccgagccgc tgcccgtgca cgcggtgtac
     2521 gcggccggcc ggcgccgccc gcccaaagtg gacgctgtga ttcagtttct ggccgaaaag
     2581 ttctcgtcgg cgccctggcg aactgcgtcg agcgcgccgc tgtccgccgc acgcggccgg
     2641 gagccggcct cgaaacgacg tggaacgcgg cgctgagctg cgcagatcac cttcggctac
     2701 cgagcggcgg agcacgtcca gtcggcacag tttccggtag tggcggggcc cggttgccgc
     2761 cgctcgcgtc atcgctcaga atgatctgaa ggcagccgtc attgcgagac ggcgaacgac
     2821 acgattcatg gcaacgcagt actacaccgc atccagcctg gacggattca ttgcgacagc
     2881 cgacgactcg ctcgactggc tgtttcagct gggtgacgtg aacgatacgg gctaccccga
     2941 cttcatccgg gacgtgggcg cactggccat ggggtcagcg acgtaccagt ggatgctgcg
     3001 tcacgtgatc aggactggca gcagttctca gggcacgtgg ccctatcagc agccctgctg
     3061 ggtgttttcg agccgggaac ttcccgccgt cgattcggcg gacatccgct ttgtccgtgg
     3121 cgatgtccga cccgttcacg aagagatgca ggcagctgcg gcaacgaaga acatctggct
     3181 tgtaggtggc ggtgacctcg tgggccagtt ctttgatgcc ggcctgctcg acgaggtcat
     3241 cgttcagatc tgctccgtga cgctgggtgc gggcaagccc ctgctaccgc gcttcatcgc
     3301 ctttccgccg ctccacctga catcggcccg cgccataggg acaggcttcg cggaattgcg
     3361 atacaaagtc ccttcgagca actgaaaggg atcgcgagcc cgacgaggac tgctcaatgc
     3421 cgactccgat atgaacatct tcgtctcgta cagccctgcg gataacgatt tggtatcggc
     3481 gatcgtgtcg cttttgaccg cgagcaatgg cgcgatcgtc tactgcgacg cagattcaat
     3541 acgcaccagc ggcagatggc gcgaagaact ggaaaaggcc atatccgaag cgaacctggt
     3601 catggtgttc tggtgtcacc actcgaacac ttcttatgaa gtgcgtaagg agttcgcgct
     3661 tgcgctcgaa cggggcaagg acgtcttgcc actgttgctg gatggaactc cattgcctgg
     3721 caagcttgcc gaccgtcgca ccatcgactt tcgtggccgg gtcgaagtga tacacgagac
     3781 gtcgcccgga caggccgaag cggggggccg ggcagacagg gcagatgcag atcgacaaat
     3841 cgccagtggc ctttcgagac agtacgaaga gctgctggtg atgggtctcg ccagggaaat
     3901 tgaaaccgag ctggccacac ggctcggaac gagatgaagt gcctttggcg tgaacaggcc
     3961 ctagaacccc atccgcatgg acgtatcgtc ctcatcttcg atgtcgacct tggctgacgg
     4021 aggcagcttc gcaatgatct cttcgatcat gcgtacgtgc tcggcctctt cgtcccgcag
     4081 ctcctcgaac agtgccttga cgtcgggttg cttcacgctg cgcagagccc tgtcgtagaa
     4141 cgcgaaagcc ttcttttcgg agtgcaacgc gacctggtag gccttgaact ccgacatgtc
     4201 gtggcgagtc gagccgacat cgggggcctc cacgtcgaag atgtcatcga gcgtgacttg
     4261 tgggggctcg ttgccaaaga gggtgcgccg gcgttcagcg agctgatcac catgcttcct
     4321 ctcgttgtcg gccatcgact ggaacaccga cccggccccg cccgaatatc cgattccaag
     4381 ctgccgggca aacagggtgt accgctcaaa ggcctcgacc tcgatcaggc tggccagatc
     4441 gagtgtgtcc atcagtgtga gcttggagaa atcgagtttc gcaggcatag gtcctccggt
     4501 tctagtgcga agcgccgtcc ttccggctgg agcagcggac tccccgtgcg tctccgtgag
     4561 gcacgcgccg ctttgctcgc ggcgaagtca tcggccaccc ggagttcccg gcgcatgagc
     4621 ccctatatct cactgcgtgg caagaaggtt gttgttgcag ggagacgatg ggtctcaaac
     4681 gagctttcga tgctaggccc acgcttggca caaacattga gcgtcgtcag aatgaatcat
     4741 tcggcagggc agccagttac aggtgcgttg gccgtccaaa caggagacag gcgatgaaag
     4801 agcgttcaaa tcgaggtcac ccggtcgcca cagcactgct gtttgcgctg gtgtcagcaa
     4861 cgacggtgtg tgccgaagca cagacgcggc cggacgaaac gcggttccgc gccacgatct
     4921 acggctactt ccccggcctc agcggaacga ccgagtttcc gagcggcgct ggcgggccct
     4981 cgatcaccgt caacgccaag gacctcctcg aagacctcaa gatggctttc atgggcacgc
     5041 tggagatgcg ttccggcaag tggggcgggt tggtggactg ggtgtatgca gatgtcgggt
     5101 cccaaaaggc cggaacgcga gaattctcga tccatggtca gcaacttccg gtgggcctga
     5161 ccgccaatct gagcctcgac gtcaagacca acatcctgac gctggctggc acgtattcgt
     5221 tcatcgagtc tccctcctac agcacgggcc tggtcttcgg cgcacgcatg ctcgacatgg
     5281 acaacacgct ggactggtcc tttgtcggga ccgggccggc cgggatcgcc cgcaccgggc
     5341 ggagcgaagt aagcgcgacc aactgggatg cgatcatcgg tgcgcgcggt cgggcccact
     5401 ttggcagcac actggcctgg ttcgtgcctt actacgtcga catcggcacc ggcaactcaa
     5461 aagtcacatg gcaggcaatg acgggcctcg gctactcgtt cgggtggggc gacgccatcg
     5521 tcgtctggcg ttacctcgac tatgagttcg attccggcga gcccacgcag tcgctgacct
     5581 tcaacggagt tgcggtcggg ctcagcttca ggttctagta ccggtcgtgc ggcagagggg
     5641 cgtgcgcagg cgtgatcccc tggatctgaa atccagccgg gagccgacgc cggacctgtg
     5701 aggcactcga ggaagcaatg tccgaatccg aagtgctgca cccattgcgc ccgattctca
     5761 agaactgggt gtgggagcat ggtcgcgtcg ggacgcgcta tctcgactgc accgatggcg
     5821 aaatcaagtt cgaagatggc aagaaggcgc gctacgcctc cgagagcatc ttctacgtgc
     5881 cgctcggggg cgaccctgga ccatccagtg aagccggacc cacgatccac gaaggagcgc
     5941 tggcgcggtt cctgaaggcc gcacagctag gcacgccgga agaggcgggt tcagtggcgg
     6001 acgtgcagcg cgccgcgcag gactgcgtgg atctctgcct cgtctgcgcc taccaggacg
     6061 atgcccaacg ggcgtttgcg cactatgcgc tcgagccgat gttcgaggac gagatccggg
     6121 ttgcggtggt cgcggacatc cgcgacaagt acctgggtct gcgggctcaa ttggcgctct
     6181 atgacttcac ggtgttttac gggctgcctg cgccactgct catcagcgaa gcgccgttta
     6241 tcgactggcg cgtgcgcggg cagccggtcc agccgttcgt gacgctaccg ctcggaccgt
     6301 attgcctgct ggtgggtacg ccgtccggaa agaaatcccg ggccggcccg gtgctctgga
     6361 aggccgcgag cgttatgggc ccgttcaagg atcacaaccg tcacattgtg gagcgcgcgc
     6421 atcgatggct cgtggcgacg tcggacgatc aactcgctgc cgtgcaggac cgatacgccg
     6481 ttccgggtcc ggaccccgcg gaagccgcga aaggttgagg gatccgggcc agctgcgatt
     6541 agccctggag cagcgtgcgc gccacctgca acagcacgaa tccgaaggtc gcgaggacgg
     6601 ccaccatcgt ggaacgcgtc gaaatcgctt cctgtaacgc aatgaaccgg cgttctcgct
     6661 tccgccggcg tttctctccg ctgcttgcac tcataacgct cctcgacggt cccccccgat
     6721 gagcgagctt ccttttgaat tctggccgga agtctcaagt aggggcagag caaaattcga
     6781 gcccaggacc taaccgcaag agaaacaaat gtttgcgtcg atggtgggtc actggcacgc
     6841 gcaacctgta aagcgcttcg acagcgctca cacgcctgga atcagcaggt tcgcaattgc
     6901 agaccttcgc tgcggggatc agaatcaatc ggattcaacc ccgccagcgc cacccactgt
     6961 gcagttcccg agcacagggg ccaccatgat cttcccgagt gtctatcgga ggcagcgatg
     7021 accgacgaaa agctcgacga attggaagca gtttgtagcg ccaaagcaga catggatggg
     7081 atggtccgaa tcgatcctgg cgacgtgctg gaactcgtgc gcgacctgcg cgaactgcgc
     7141 aagcacgccg ctggcctgac cgcgcttgaa ggggcggtcg gcaccatgca ccgactgctg
     7201 ggcgacctgt gcgcctacgg gacgcgttcg ccggacatcg acgtcgtcaa ccgtattgcg
     7261 gttcaggacc cgggtcgctg aaactctcgc tgcgcagcga gggcagcttc agcgacgaac
     7321 agctgctgcg ttcgaaccac gacgacccgg tccgattgaa cggcttcggg cggacagcat
     7381 tcgccgtcac ttgcggaccg gattggcgaa attggcgttg cacatcgaca ggaaagggaa
     7441 ctgcatatga caacctcacg gatcgcagca acccttgcag gaacgattgc actcgcggtg
     7501 ctgggtacgc tggcgctgct cgcgggcagt ggcggaaagg ccgtcgccca gcagtcacct
     7561 cctgcctgca cctgtgcgcc cgtgacgtct gtggcctcga tcgggacgaa tcttgtccac
     7621 tgccagtgtg gtccggcgac ctgcgtcgtc agcgagcatt tcacggggca gtcaaagacc
     7681 tatgccctcc agtgcgccaa gtgagccgat caccgttcag ttgagcggga tcgccctcgg
     7741 cctccggtgc aggcgaagtc aggcaacagc actgacaacg tgtcggccga aggtccaatg
     7801 ccacatcctg aagagggccg ggagccgccg gcaggccaat tgacacaagg acaagcgtcg
     7861 aatgcgcggc ggagcatgct gaaggtggtt tgaagtgaac agaaagtcat gtctgcagtc
     7921 gcgcacacgc accatctggg cacgggcttc tggcgcctcg cggaccccaa ggtcacgctg
     7981 gcgtcgatgt cgtcgatcct gctcggcgcc gcggcggccg ttcaaagcgg cacgtacgcg
     8041 ttcagttggc tgatcctcgt ggtggtcggc atctttgcac tggagtgggc caagaacgcc
     8101 tcgggcgaga tctatgactt cgcttcgggc gccgatcagg ccgtcgccga gtcggaccgc
     8161 agtccgtttt ccggcggcaa gcgggtgctg gtcgacggtc tgcttacgga acgggagacc
     8221 tggaccctag cgatcgtcgg ctatgccctc tgcataggcg cagggatcat c
//
LOCUS       DATIRT010000052         4624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_596400, whole genome shotgun sequence.
ACCESSION   DATIRT010000052 DATIRT010000000
VERSION     DATIRT010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596400"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..131)
                     /locus_tag="VLN25_01220"
     CDS             complement(<1..131)
                     /locus_tag="VLN25_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor"
                     /protein_id="HSQ09228.1"
                     /translation="MASVASAVEETIGRTVEGLGYEFVDAERLAAGLLRVTIDRAGG"
     gene            complement(379..1677)
                     /locus_tag="VLN25_01225"
     CDS             complement(379..1677)
                     /locus_tag="VLN25_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HSQ09229.1"
                     /translation="MNTQVMTASTESPPLPLAGEGDDINPAAMADVALPREVLDRGRR
                     AVKQALNAQSEKLHKVLADAGIGSRRDMEELIIAGRVSVNGQPAHTGQRVMPNDQVRV
                     NGKPLQRRPAGGRPPRVLLYHKPVGEIVTQDDPGERPTVFEKLPKVSGGRWVAVGRLD
                     LNTEGLILFSTSGELANRLMHPRYEVEREYAVRVLGEVSETQRQQLLDGVQLEDGAAK
                     FSKVEDAGGQGVNHWYRVALNEGRNREVRRIFEAIGLTVSRLIRIRFGAIQLPRSLAR
                     GRFYELAPEWVQAWVHDLGMAIDEVRSRGPSQPHSGKGGRSKGGPGRGRKPGQGPNLG
                     LTPGGQSYSPISGYGQGQGYGQGPAYGQGKGFGQGQGQGQGQGQGQGQGRGKGKPQGN
                     RPPKQPDPMTSSVNYIAAGHGLPNNGRPARFKRGKPQRTY"
     gene            complement(2136..2642)
                     /gene="scpB"
                     /locus_tag="VLN25_01230"
                     /pseudo
     CDS             complement(2136..2642)
                     /gene="scpB"
                     /locus_tag="VLN25_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004534439.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
     gene            complement(2984..3184)
                     /locus_tag="VLN25_01235"
     CDS             complement(2984..3184)
                     /locus_tag="VLN25_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09230.1"
                     /translation="MRKEGRVVIGARYADKGFIVMTGASANDVRALLDADPAVQRRVF
                     DVEVNEFHVFYAGCVATASPRR"
     gene            complement(3261..3749)
                     /locus_tag="VLN25_01240"
     CDS             complement(3261..3749)
                     /locus_tag="VLN25_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HSQ09231.1"
                     /translation="MSDADITGFEGGCTCRQVRYRMSGPPLFVHCCHCRWCQRETGAS
                     FALNALIEATRVQLLEGDVELVDTPSNSGKGQKIARCPKCRVAVWSNYSGAGDAVRFV
                     RVGTLDDPDRFPPDIHIYTASKQPWVQLPPGIAAVPEYYRASEHWPKSSLERRAALLA
                     AR"
     gene            complement(3828..4205)
                     /locus_tag="VLN25_01245"
     CDS             complement(3828..4205)
                     /locus_tag="VLN25_01245"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSQ09232.1"
                     /translation="MRENRLTSRAISDDEVAHAIEVSGRGWVVEVDRTIVGFAIGNAQ
                     DGNIWASFIDPEHQGHGHGRRLHDTMVAWLWSRGLRKLWLCTEPGTRAQRFYSSAGWT
                     NRGLLPSGELRFELYRRDGASNA"
     gene            complement(4311..4622)
                     /locus_tag="VLN25_01250"
     CDS             complement(4311..4622)
                     /locus_tag="VLN25_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HSQ09233.1"
                     /translation="MQFEIATDFPELTMCDCSICRRKNALMVKVHESRFRLLAGEDAL
                     TEYQFHTRTARHYFCRVCGIYPFHRKRVTPDFLGINVHCLHDFDPTGIPVRQAIGAAK
                     P"
BASE COUNT          792 a         1544 c         1465 g          823 t
ORIGIN      
        1 atacccccag cgcgatcgat cgtcacgcgc agcaagccag ccgcgaggcg ctcggcatcg
       61 acaaactcgt atccgagtcc ctcgacagtg cgtccgatcg tctcttccac cgcgcttgcc
      121 acacttgcca tcgcccgatc actccttcac ggcgtggcca cgaaatctgt cgagccgccg
      181 caaaacgccg cccggaaata aaaaatgggc tcgagagccc atccgttgaa ttcaacaggc
      241 ttgcgcagca actgcggtgc cacgcaaccg acgcccaccg gatcgaatct cgatcggcgc
      301 gcactccggc aaccgcgcat tataggggcc taaccgcgct gcaacaagcg cccggcggcg
      361 gcagccgact cggacctgct agtacgtgcg ctgcggcttg ccccgcttga aacgcgccgg
      421 ccgaccgttg ttgggcaggc cgtggccggc cgcaatgtag ttgacgctgc tggtcatcgg
      481 atcgggctgc ttcggggggc ggttcccttg cggcttgccc ttgccacggc cttggccctg
      541 gccctgccct tgcccctggc cctggccctg gccctggcca aagcccttgc cttgcccgta
      601 cgccggaccc tggccgtagc cctgcccttg gccgtagcca ctgatcgggc tatagctttg
      661 cccgcccggc gtgagtccga ggttcgggcc ctggccgggt ttgcgtccgc ggcccggccc
      721 acccttgctg cgaccgccct tgcctgagtg cggctggctg gggccacggc tgcgaacctc
      781 gtcgattgcc atgcccaggt cgtgcaccca ggcctggacc cactcgggtg caagctcgta
      841 aaacctcccg cgcgccaggc tgcggggcag ctggatcgcg ccgaagcgga ttcggatcag
      901 tcgactgacc gtcaagccga tcgcctcgaa gatgcgccgg acttcgcggt tgcgcccttc
      961 gttgagtgcg acccggtacc agtggttcac accctggccg ccggcatcct cgaccttgct
     1021 gaacttggcc gcaccatctt cgagctgcac accgtccagc agttgctggc gctgggtctc
     1081 ggagacttct ccgagcacgc gtacggcgta ctcgcgctcg acctcgtaac gcgggtgcat
     1141 cagccgattg gcgagttcgc ccgacgtcga aaacaggatc agaccctcgg tattcaggtc
     1201 cagccgaccg acggcgaccc agcgtccgcc agagaccttg ggcaacttct caaagaccgt
     1261 cgggcgctct ccgggatcgt cctgcgtgac gatttcgccg accggcttgt gatacagcag
     1321 aacacgcggc ggccggccac cggcgggccg gcgctgcagc ggcttgccgt tgacgcgcac
     1381 ctggtcattg ggcatgacgc gctgccccgt gtgcgcaggc tgtccgttca ccgaaacccg
     1441 gcccgcgatg atcaactctt ccatgtcgcg gcgcgaaccg atgccggcat ccgccaggac
     1501 cttgtgcagt ttttcggact gcgcgttcag tgcctgcttg actgcgcggc gcccgcggtc
     1561 gagcacctcg cgtggcaagg cgacgtcggc catcgcggcg ggattgatgt cgtcgccctc
     1621 tccggccagt ggcagcggcg gagattcggt cgatgccgtc atcacctgag tgttcaccgg
     1681 tgcaggacga tccgcatcac tgcgtccacc gcgcccgcgt cgacgccgac catgcggccc
     1741 gcgctgtttc ggagtgtctt cacccgcggc ttcggagtcg cctgagggcg cgatggcgac
     1801 attgccgtcg acgtcgtccg ggtgttctcc ggtgggctcg tcatcatgcg gggacgcagc
     1861 cctcgcccca ctttcggcag gactcaccgg attcgtcgga gtttgatcgt cgtcgggggt
     1921 cgaggatgga ttcatcagga tggaagtcag gcgggagtcg cggccgagga ggtcgatgag
     1981 gtcgcggtat cggcggagta ctcggcgtcc accgccgcaa tcgccgcata cgacggctca
     2041 gcgtcgacct cggcggaagt ttcgcgcgcc tgcgccccat cggagcgcac ggcatcggcg
     2101 aattgaagct cgaactcggg ggccactgtc gcgccctctt cgaggatggg cagttcgtcc
     2161 agtgaacgca gaccgaggtc gtcgaggaac gagcgcgtag tcgcgaacaa ggccggccgg
     2221 cccggcgctt cacgatggcc gatgacctcg atccagcccc gctcctccag tgtcttgata
     2281 actggcgccg atacggtgac accgcggata tcctcgatgt cgccgcgcgt gacgggctga
     2341 cggtaagcaa tgatggccag cgtttcgagc accgcgcggg agtacttcgg tgctttctcc
     2401 ggattcagcc ggtcgagata cggacgcatc gatgcagcac tctggaagcg ccagcccgac
     2461 gcaagtgccg cgagttcgat accgcgaccg gcccagtcgg cgcgcaactc gtcgagcagc
     2521 acgcgcaggg tgtcggcatt gacctcgtca ttgaacatcc gccgcaattc gctgagcgaa
     2581 agtggctgct cggcggtgag tagcgccgct tcgagcacgt tcttggcctc ttgtgtattc
     2641 atgtctcccg ttcggagaga agctggcaat ggcactgctc acgtcgcgct tcggtccgca
     2701 acaagacccg aagcgtgcct gctgaagcac gtcaattgca tggaaatctt ggaagcggag
     2761 ttcgaagacc gcggagagcg gcgggcgtgt cgttgttgga gcctgccgag cgcggagtcg
     2821 gacgatccgg caactcttgt cggcccgggt cgaaaaagac gcgcagtacc gaccgaaaat
     2881 ctgactgaaa tcagtctatc acagcaatct ctggcgagaa ggcgcccggg cgcgattcat
     2941 ccaatcggtc gagcgatgca tcgccgcgac aggttccgat gcctcagcgt cgcgggctgg
     3001 cggtggcgac gcagcccgcg tagaagacgt ggaactcgtt gacctcgacg tcgaaaacgc
     3061 gccgctgcac ggccgggtcg gcgtcgagca acgcacgcac gtcgttcgcc gaggcaccgg
     3121 tcatgacgat gaatcctttg tcggcgtagc gcgcgccaat gacaacgcga ccctccttcc
     3181 gcaaccgggc aagattggtg gagtggattt atggtcgatc tccggccaat gatttgctgt
     3241 gcagctttcc ttcgatgacc tcaccgcgcc gccagcaagg ccgcacggcg ctcaagactg
     3301 gacttcggcc agtgttctga cgcccggtag tactcgggga cggccgcaat ccctggcggc
     3361 aactgcaccc atggctgctt cgacgccgta tagatgtgga tgtcgggggg aaagcgatcc
     3421 ggatcatcaa gggtcccgac acgcacgaag cgaaccgcgt caccggcgcc ggagtagttg
     3481 ctccagaccg ccacgcggca cttcggacag cgtgcgatct tctggccctt gccgctgttc
     3541 gatggcgtat cgacgagctc gacgtcacct tcgagcagtt gcacccgcgt ggcctcaatc
     3601 agggcgttga gtgcgaacga ggcgccggtt tcgcgctggc accagcggca atggcagcaa
     3661 tgcacgaaca gcggcggacc ggacatccgg tagcgaactt ggcgacaggt gcacccgcct
     3721 tcgaaccctg tgatatcggc atcgctcatc cgagtctccc gcgttcgacc tgatgtccgt
     3781 acaagcacga catgccacgg gcgcggcggc tcgaagcaac acggcagtca cgcattcgat
     3841 gctccatcac gtcggtacag ttcaaaacgc agctcgccgc tgggcagcag cccccggttg
     3901 gtccaccccg cgcttgagta gaagcgctgc gcgcgggtgc cgggctcggt gcacagccag
     3961 agcttccgca agccgcgcga ccatagccac gccaccatgg tgtcatgcag ccgtcgcccg
     4021 tggccatgac cctggtgctc cggatcgatg aacgacgccc agatgttgcc gtcctgcgcg
     4081 ttgccgattg caaagccgac gatggtgcgg tcgacctcga ccacccatcc gcgccccgac
     4141 acctcgatgg cgtgcgcgac ctcgtcgtcg ctgatcgcgc gcgaggtcag acggttctcc
     4201 ctcactgcat gtcgcacccg ctggataccg caaatgtcgg atcggttggc ctgccgctag
     4261 acggcacccg tcactgccga attcttttcg cgagtcatgg ccgccgcgac tcacggcttc
     4321 gccgcgccaa tggcctgccg caccgggatg ccggtgggat cgaagtcatg caggcaatgg
     4381 acgttgatac cgagaaagtc aggagtgacc cgcttgcgat ggaaaggata gataccgcag
     4441 accctgcaga agtagtggcg cgcggttcgc gtatggaact ggtactccgt gagcgcgtct
     4501 tcaccggcca gcagacggaa gcggctctcg tgtaccttga ccataagcgc gttcttgcgt
     4561 cggcagatgg aacagtcaca catcgtgagt tctggaaaat ccgtggcgat ctcgaactgc
     4621 accg
//
LOCUS       DATIRT010000053         4240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_792847, whole genome shotgun sequence.
ACCESSION   DATIRT010000053 DATIRT010000000
VERSION     DATIRT010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792847"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VLN25_01255"
     CDS             <1..783
                     /locus_tag="VLN25_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HSQ09234.1"
                     /translation="RGTAHVLLAAVIEFIHTATLLHDDVVDESDLRRGRTTANARFGN
                     AASVLVGDFLYSRSFQMMVDAGSMRVMRILADATNRIAEGEVLQLLNVHDPSVNEERY
                     FSVVERKTATLFEAGARIAAVLAEADASTEERCAKYGASLGRAFQIVDDMLDYSGHTE
                     DIGKRLGDDLREGKVTLPLIHALRSATAEQRELVVHAVREGHGDFDAIARIVADNGSI
                     AYSGRLASREVEIADRAIRELPASVYRDSLLNLLAFAVGRDR"
     gene            826..902
                     /locus_tag="VLN25_01260"
     tRNA            826..902
                     /locus_tag="VLN25_01260"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:860..862,aa:Pro,seq:cgg)
     gene            953..2245
                     /locus_tag="VLN25_01265"
     CDS             953..2245
                     /locus_tag="VLN25_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HSQ09235.1"
                     /translation="MDELPLWVALVGLAILLLISAFFSISETAMMALSRLRLRHMARE
                     GLRSAIRVQSLLDHTDRLLGTILLGNNLVNAAATALVTAMAIRFVGDNEWALLAATGV
                     VTFLILVFAEITPKVIGATYPEKIALPASRPLKLLLAAFRPVVWFVNLFVKSLLWLLR
                     VPSVRGGEATRLTAEELRTLVLEGGNFIPGKHRSILLNLFELEQLSVDDVMSPRARIE
                     SLNLMDSRHAVLEQLRTCYHNKLPVHEGDINRTVGILLVRKLLPMLSSDDFSIEALRG
                     ALVQPYYIPSGTPLFQQLQLFQDNQQRLGLVVDEYGEVRGLVTLEDIIEEIVGEFTTR
                     MPSSGSHLDWGTDGTVLVEGGITLRELNRRLALRLPLDGPKTLNGLLVERLEEIPDAP
                     CSVRFGSVVIEVVQIDEHAIRSARIARLRPTSTRRQEA"
     gene            2422..4170
                     /gene="pilB"
                     /locus_tag="VLN25_01270"
     CDS             2422..4170
                     /gene="pilB"
                     /locus_tag="VLN25_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HSQ09236.1"
                     /translation="MSAVTQGPGAAAGALTGLARALVQQGKLRPDRAENFARKAAQSN
                     TQFIDELINSADVTGLTASGVAKFAAETFGHPMLDLSAVDLEQLPRDIIDKKLIATQR
                     VLALRKRGNRLAIAVSDPTNFPALDQVKFQTQLALDVIVVEHDKLLRLVESQAQSVNQ
                     KLEDMVGSDDVNFDDLLGESPEQAGPDEAAQVEVDDAPVVRFLQKLLLDAIGEGASDL
                     HFEPYEKFYRVRFRIDGVLREIAQPPLAIRERLSTRIKVVSRLDISEKRVPQDGRMKL
                     ALSGQRAIDFRVSTLPTMFGEKIVMRILDPSQAKLGIEALGYEPDQKEVLLDAVQRPY
                     GMVLVTGPTGSGKTVSLYTCLNILNQPGVNISTAEDPAEIQIAGINQVNVNEKAGLTF
                     ANSLRAFLRQDPDIIMVGEIRDLETADIAIKAAQTGHMVLSTLHTNDAPGTLVRLQNM
                     GVAPFNVASSVILITAQRLARKLCACKQPAEVKKDALVTAGFRGEDLDGNWIPYKAVG
                     CEKCKGSGYKGRLGIYQVMPITETMQDMILRLATAPEIAREAAANGVRDLRQSGLIKV
                     KAGITSIDEVLGCTNE"
BASE COUNT          826 a         1318 c         1305 g          791 t
ORIGIN      
        1 cgcggaacgg cccatgtgct gctggcggcg gtcatcgagt tcatccacac ggcgacgctg
       61 ctgcacgatg atgttgtaga cgagtccgac ctgcgacggg gccgaaccac cgccaacgcc
      121 cgtttcggca atgccgccag cgtcctcgtg ggtgacttcc tgtactcgcg atcgttccag
      181 atgatggtcg atgccggaag catgcgggtc atgcggatcc tcgcagatgc caccaaccgg
      241 atcgccgagg gcgaggtcct gcaactgctc aacgttcatg acccatcggt gaacgaagag
      301 cgctacttca gcgtggtcga gcgcaagacc gcaacgctgt tcgaggcggg agcccgcatc
      361 gccgcggtcc tggccgaagc cgatgcctcg accgaggagc gctgcgcaaa atacggcgcg
      421 tcacttggcc gggcattcca gatcgtcgac gacatgctcg attactcagg ccacaccgag
      481 gacatcggca agcggctggg cgacgacttg cgcgaaggca aggtgaccct gccactgata
      541 catgcactgc gcagtgccac agccgagcaa cgcgaactcg tcgtgcatgc ggtgcgggag
      601 ggtcacggcg actttgacgc gatcgcccgc atcgtggcgg acaatggctc gatcgcctat
      661 tcgggaagac ttgccagccg ggaagtcgag atcgcggacc gtgcaatacg cgaacttccc
      721 gcctcggtgt atcgagattc tttgctaaac ttgctggctt tcgcggtggg ccgcgatcgg
      781 taaactctcc gatcacgaag agccattggc gtgccacgcg accgtcgggg tgtagctcag
      841 cctggtagag tactgcgttc gggacgcagg agtcggaggt tcaaatcctc tcaccccgac
      901 cacttcactt cctctcttcc cttctgatcg ggtaattccc ggccctcggc acgtggacga
      961 attacccctc tgggtcgcac tggtcggcct agcaatactg ctgctgattt cggccttctt
     1021 ctcgatctcc gagacggcaa tgatggcgct cagccgcctg cggttgcgtc acatggcccg
     1081 cgaaggcctt cgctccgcga tccgagtcca atccctcctc gaccacactg acaggctgct
     1141 cggcacgatc ctgctgggca acaacctcgt caatgccgcg gccacggccc tcgtcacggc
     1201 catggcgatc cgattcgtag gagacaacga gtgggcactc ctggccgcca cgggagtcgt
     1261 gactttcctc atcctcgtgt ttgcagagat cacgcccaag gtcatcggcg ccacgtatcc
     1321 ggagaagatt gcgctgccgg ccagccgacc actgaagctc ctgctggcgg cgtttcgtcc
     1381 cgtcgtctgg ttcgtcaatc tgttcgtcaa gtcgctgctg tggctgctgc gtgtgccgtc
     1441 ggtgcgaggt ggcgaggcaa cccgattgac ggcggaagaa ctgcggacgc tggtgctgga
     1501 aggcgggaac ttcatcccag gcaagcaccg cagcatcctc ctgaacctgt tcgaactgga
     1561 gcaactctcg gtcgatgatg tgatgtcacc gcgtgcgcgc atcgaatcgc tcaacctgat
     1621 ggattcgcgc cacgccgtgc tcgagcagct tcgtacctgt taccacaaca agctgccggt
     1681 acacgagggc gacatcaacc gcacagttgg aatccttctg gtgcgcaagc tgctaccgat
     1741 gctttcgagt gacgacttct cgatagaagc gctgcgggga gcactcgtac agccctacta
     1801 catccccagc ggcactccgc tcttccagca attgcagctc ttccaggaca accagcagcg
     1861 tctgggtctg gtggtcgacg agtatggcga ggtccggggt ctcgtgacac tcgaagacat
     1921 tatcgaggag atcgtgggcg agttcacgac acgaatgccg tcctcggggt cgcacttgga
     1981 ttggggcacc gacgggacag tgctggtcga gggtggtatc acactgcgtg agttgaatcg
     2041 tcgcctggcg ctcagactgc cgctcgacgg accgaagacg ctcaatggct tgctggtcga
     2101 gcgcctagaa gagatccccg acgcgccgtg cagcgtacgt ttcggcagcg tggtgatcga
     2161 agtggtgcag atcgacgagc acgcgatccg cagtgcccgt atcgcccggc ttcgtccgac
     2221 aagtacacgt cgccaagagg cctgaaacgc acagcaagat ggcccgctga agcgcgccgg
     2281 attgctcgtt cggcttgcct tgtttgcggg agttaagctt tacaaatccg ggccttatcg
     2341 gcagtatctt tttgcttcgt gagaagtgcc cgcccgctat aacttgggtc ccatcggatc
     2401 cctcagggac agcaccatcc catgtctgcc gtaacgcaag gtcctggcgc cgccgcaggg
     2461 gccctcaccg gtctcgcgcg tgcacttgtc cagcagggaa agttgcgccc ggaccgggcc
     2521 gagaactttg cacgcaaggc ggcgcagtca aacacccagt tcatcgacga gctgatcaac
     2581 tccgcggatg tgacggggct gaccgcgagc ggcgtcgcca aattcgcggc cgagactttc
     2641 ggtcatccga tgctggatct gtcggcggtc gacctcgaac agctgccgcg cgacatcatc
     2701 gacaagaagc tcatcgccac tcagcgcgtg ctggcgttgc gcaaacgcgg caaccggctc
     2761 gcgatcgccg tatccgatcc gacgaatttt ccggcgcttg accaagtcaa gttccagacc
     2821 cagttggcgc tcgatgtcat tgtcgtcgag cacgacaagt tgttgcgcct ggtcgagtct
     2881 caggcgcagt cggtcaacca gaagctcgag gacatggtcg gcagcgacga tgtcaatttc
     2941 gacgatctgc tgggcgagag cccggagcaa gcaggcccgg acgaagcggc acaggtcgag
     3001 gtcgatgacg ctccggtcgt ccggttcctg cagaagctgc tgctcgatgc gatcggcgag
     3061 ggcgcatccg atctgcactt cgagccctac gagaagttct atcgcgttcg cttccgcatc
     3121 gacggcgtct tgcgcgagat cgcgcagccg ccgctcgcta tccgcgaacg cctgtcaacc
     3181 cgcatcaagg tcgtttcgcg gctcgacatt tccgagaagc gagtgcctca ggacgggcgg
     3241 atgaagctcg cgctctccgg ccagcgcgcg atcgatttcc gcgtcagtac cttgccgacg
     3301 atgtttggcg agaagatcgt gatgcggatt ctcgatccgt cgcaggcgaa gctcggcatc
     3361 gaggccctgg gatacgagcc ggaccagaag gaggtcctgc tggatgccgt ccagcggccg
     3421 tacggcatgg tgctggtgac cggaccgaca ggctccggca agaccgtgtc gctctacacc
     3481 tgcctgaaca tcctgaatca gccgggcgtc aacatctcca cggcggaaga cccggcggaa
     3541 attcagatcg cgggcatcaa ccaggtcaat gtaaacgaaa aggccgggct gacgttcgca
     3601 aactccttgc gtgcgtttct gcggcaggac ccggacatca tcatggtggg cgagattcgc
     3661 gacctcgaaa ccgccgacat cgcgatcaag gccgcgcaga ccggacacat ggtgctgtcg
     3721 acgcttcaca caaacgatgc cccgggcaca ctggtgcgtc ttcagaacat gggggttgcg
     3781 cccttcaatg tcgcctccag cgttatcctg atcactgccc agcgcctcgc ccgcaaactc
     3841 tgcgcttgca aacaaccggc cgaggtcaag aaggatgcgc tggtcacggc tggtttccgg
     3901 ggcgaggatc tcgacggcaa ctggattcct tacaaggcgg tcgggtgcga aaaatgcaag
     3961 ggcagcggtt acaagggcag gctgggcatc tatcaggtga tgccgatcac cgagacgatg
     4021 caggacatga ttctgcgtct tgcgacagcg cctgagattg cgcgcgaggc ggcagcaaac
     4081 ggcgtgcgcg atctgcgtca gtccggatta atcaaggtca aggcgggcat cacctcgatc
     4141 gacgaagtgc tcggatgcac caacgaatga tgtagggcag gagagacgtc aacatggcca
     4201 ccggcgcaat tgcaaaaccc aaaccccgcg cagaagtgcg
//
LOCUS       DATIRT010000054         5361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_792859, whole genome shotgun sequence.
ACCESSION   DATIRT010000054 DATIRT010000000
VERSION     DATIRT010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5361
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792859"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..141
                     /locus_tag="VLN25_01275"
                     /pseudo
     CDS             1..141
                     /locus_tag="VLN25_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929988.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
     gene            complement(129..305)
                     /locus_tag="VLN25_01280"
                     /pseudo
     CDS             complement(129..305)
                     /locus_tag="VLN25_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929988.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
     gene            784..1095
                     /gene="rplU"
                     /locus_tag="VLN25_01285"
     CDS             784..1095
                     /gene="rplU"
                     /locus_tag="VLN25_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HSQ09237.1"
                     /translation="MYAVIKTGGKQYRVAAGDTLKVETLAVDVGQQIKLPEVLAVVDG
                     GTLKTGTPLISGAAVVATVLAHGRRDKVTIFKMQRRKHFQKHAGHRQNFTELRIDSIA
                     A"
     gene            1131..1391
                     /gene="rpmA"
                     /locus_tag="VLN25_01290"
     CDS             1131..1391
                     /gene="rpmA"
                     /locus_tag="VLN25_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HSQ09238.1"
                     /translation="MAHKKAGGSSRNGRDSQAKRLGVKVFGGQSISAGAIIVRQRGTQ
                     FHAGDNVGMGRDHTLFAKSDGAVKFEVSGAQNRRVVNVVLAG"
     gene            1462..2529
                     /gene="obgE"
                     /locus_tag="VLN25_01295"
     CDS             1462..2529
                     /gene="obgE"
                     /locus_tag="VLN25_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HSQ09239.1"
                     /translation="MKFVDEARIEVSAGKGGDGCASFRREKFIPKGGPNGGDGGRGGS
                     VWARADRNINTLIDYRYARRHRANSGQSGRGADCYGAGGADIELRMPVGTVVFDDDTG
                     EELADLAHHGDRALLAAGGRGGLGNLHFKSSINRAPRQFTKGEPGEHRQLRLELRVLA
                     DVGLLGMPNAGKSTFIRSVSNARPKVADYPFTTLHPHLGVVRVGPERSFVVADVPGLI
                     EGAAEGAGLGHQFLRHLSRTGLLLHVIDCVGPDGSADPVHDARVIGGEIKKYSPALAR
                     KERWFVLNKADLVPADERDSLLANFRRRMRTKAPIHFISAATGEGCRELVVAIQTFLD
                     RRRTQEAAPAVSQDVAAGAEG"
     gene            2529..3644
                     /gene="proB"
                     /locus_tag="VLN25_01300"
     CDS             2529..3644
                     /gene="proB"
                     /locus_tag="VLN25_01300"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HSQ09240.1"
                     /translation="MTALQAARRAVVKVGSSLVTNEGRGLDIQAMARWAQQIATLRAQ
                     GKQIVLVSSGAIAEGMQRLGWARRPQQIHELQAAAAVGQMGLAQAYETQFRELGIHSA
                     QVLLTHADLADRARYLNARSTLFTLLGLGVVPVINENDTVVTDEIKFGDNDTLAALVT
                     NLVEADVLVILTDQAGLYSADPRHDPQAKLVPSAIAGVPELEAMAGGAGSAIARGGML
                     TKVLAAKRAARSGAHTLIASGREPDILLRLAAGEAIGSELKALTPVLTARKQWLADHL
                     LLKGRVVVDDGAAKALASGGKSLLPIGVIEVEGDFQRGDAVGCVTREGREIARGLANY
                     SSSDARQIARRPTSDIQGILGFIEEPELIHRDNLVLL"
     gene            complement(3653..4252)
                     /locus_tag="VLN25_01305"
     CDS             complement(3653..4252)
                     /locus_tag="VLN25_01305"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HSQ09241.1"
                     /translation="MLDKDGYRPNVGIILLNGRNEVFWGKRVKQHSWQFPQGGIKHGE
                     SPEQAMYRELWEEVGLKTEHVTIVGRTRDWLRYDVPDQWIRRDLRGNYRGQKQIWFLL
                     RLVGRDCDVCLRASTHPEFDAWRWHDYWVPLDTVIEFKRDVYQQALLELSRYVFKPGR
                     PHHKAMRSRSRDVALDGERTGTAPSVFEASDTRAGGEGA"
     gene            4337..>5361
                     /gene="proS"
                     /locus_tag="VLN25_01310"
     CDS             4337..>5361
                     /gene="proS"
                     /locus_tag="VLN25_01310"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HSQ09242.1"
                     /translation="MRARDFLISTLKEAPADAEVVSHSLMLRAGMIKKLAAGIYTYMP
                     MGLRVIRKIESIIREEMNRAGAVELLMPVIQPAELWMESGRWHKMGPELLRLKDRHER
                     DFIVQPTSEEVVVDIVRDEIRSYKQLPLNLYHIQTKFRDERRPRFGVMRGREFTMKDA
                     YSFDRDEDGAMTSYRAMYDAYVRIFQRMGLRFRAVAADTGNIGGKASHEFQVIADTGE
                     DAIAYCPTSDYAANVEMAEAVPLTAHRVAPTQPLTKTPTPGKEKCEDVAELLGLPLSQ
                     TVKSIVLAVERGDAEKPTEPEVWLVMIRGDHELNEIKVSKVPGLDQGFRFATEAEIVD
                     HFGCRPG"
BASE COUNT         1009 a         1599 c         1788 g          965 t
ORIGIN      
        1 cgcggaacgg cccatgtgct gctggcggcg gtcatcgagt tcatccacac ggcgacgctg
       61 ctgcacgatg atgttgtaga cgagtccgac ctgcgacggg gccgaaccac cgccaacgcc
      121 cgtttcggca atgccgccag cagcacatgg gccgttccgc ggtagccgag ggcgcgggca
      181 atcaggagca cgacggctgg acgaagccgc ttcccgcccg catcaatgat gtagtcggca
      241 atcgtgttga tgagcacgac atcggaactg agtcgttccc ggatgatggc gtcgactgcc
      301 tgcatgtcgg ccgcgatcgg cgcgagcagg gtcttgagct ccggggtcgt cacaccgatt
      361 gccgacggct gctccggtgt cgccgcaaac gccggtcttg ccgtccgaga gtcctggtgc
      421 aggggggggc gctccatcgg ggcattatag gagcgctctt tgacgaacct ggctgccggg
      481 tggtctcggt ggatcaatgt caccgtcgcc accttaggtt tgacgcccgc cctgggaaag
      541 tgtgataatt caaggctttc gagcagcagc cctgtccgaa acatcatcct cggctgccga
      601 cgcgggcatc gtggtgccgg accctcgcag gtttgctact ttccttagaa acagcaaacg
      661 cgacgcctct aagttgcaca gcgggcggtc atgggcagga cggattgtca ggtcgaagtt
      721 acagtctgaa aatggatttg cttcgcgcgg cccatggggt cggtggagca tgggagcgtg
      781 acgatgtatg cggtgatcaa gacaggcggc aagcagtatc gggtcgctgc gggtgacacg
      841 ttgaaggtgg aaacgctggc ggtggacgtc ggtcagcaga tcaagttgcc ggaggtcctc
      901 gccgtggtcg atggcggcac cctcaagaca ggtactcctc tgatttcggg agccgcggtt
      961 gtcgcgaccg tgcttgcgca tggccggcgc gacaaggtca cgattttcaa gatgcagcgg
     1021 cgcaagcact tccagaagca tgccgggcat cgtcagaatt tcaccgagct gcgcatcgat
     1081 tcgatcgcgg cctgataccg tagacagtta gctgatagag cgagagcacg atggcacaca
     1141 agaaagcagg cggctcctcc cggaacgggc gcgattcgca ggcaaagcgc ctgggggtca
     1201 aggtctttgg tggccagagc atttccgcgg gcgccatcat cgttcgccag cgaggaacgc
     1261 agtttcacgc gggcgacaac gtcgggatgg gccgtgacca cacgctgttt gcaaagtctg
     1321 acggcgcggt caagttcgag gtcagcggcg cgcagaatcg gcgcgtcgtg aacgttgtcc
     1381 tggcgggctg aacacccgag gcggcgtccg gtttgatcga agccctgccg tcgtcggcag
     1441 ggctttttta ttggtgagcc tgtgaagttt gtcgacgaag cgcgtattga agtctctgcc
     1501 ggcaaaggtg gggatggctg cgcatcgttt cgtcgcgaaa agttcatccc caagggtggg
     1561 cccaacggtg gcgatggcgg gcgcggcgga agcgtatggg cgcgggccga tcgcaacatc
     1621 aacacgctga tcgactaccg atatgcgcgg cgacatcggg cgaatagcgg ccaatctggc
     1681 cgcggcgcag actgctatgg cgcaggcggc gccgacattg aacttcggat gccggtggga
     1741 accgtggtat tcgacgacga caccggtgaa gagctcgccg accttgcaca tcacggcgat
     1801 cgcgcattgc tggccgccgg tgggcgcggt ggcctcggga acctgcactt caagtcgagc
     1861 atcaatcgcg cgccgcgcca gttcaccaag ggcgagcctg gggaacatcg gcagttgcgt
     1921 ctcgaactca gggtgctggc ggacgtggga ttgctcggca tgccaaacgc gggcaagtcg
     1981 acgttcatac ggtcagtgtc caatgcccgt cccaaggttg ccgactatcc cttcactacg
     2041 ctgcaccctc atctaggcgt cgtgcgagtt ggacccgagc gtagcttcgt ggtcgccgac
     2101 gtgccggggc tgatcgaagg tgcagccgag ggagcagggc tgggtcacca gtttctgcga
     2161 cacctgagcc ggacgggact gctgctgcat gtcatcgact gtgttgggcc cgacggttct
     2221 gcggatccgg tgcacgatgc tcgtgtcatc ggtggcgaga tcaagaaata cagtccggcg
     2281 ctggcgcgca aggagcgctg gttcgtcctg aacaaggccg atctcgttcc cgcggacgag
     2341 cgggattcgc tccttgcaaa tttccggcgc cggatgcgaa ccaaggctcc aatccacttc
     2401 atctccgcgg caacggggga gggctgccgc gaactcgtag tggctataca gacattcctc
     2461 gaccggcgtc ggactcagga agccgcgccc gcggtttcgc aggatgttgc ggccggagct
     2521 gaaggttgat gactgcgctg caggccgccc gacgtgcggt ggtcaaggtt ggaagcagcc
     2581 ttgtcaccaa tgagggccgc gggctcgata tccaggcgat ggcgcggtgg gcgcagcaga
     2641 tcgcgacgct gcgcgcgcag ggcaagcaga tcgtgctcgt gtccagcggc gcgatcgcag
     2701 agggcatgca gagactcggt tgggcgaggc gaccgcagca gatccacgaa ctccaggccg
     2761 ctgccgccgt cgggcagatg ggactcgccc aagcctacga gacgcagttt cgcgaactag
     2821 ggattcacag cgcgcaggtg ctgctcacac atgccgacct ggcggaccgt gcccgctacc
     2881 tgaatgcacg atcgacgctt ttcaccttgc tcggtctcgg cgtggtgccc gtcatcaatg
     2941 aaaacgacac ggtcgtgacg gacgagatca agttcgggga caacgacacg ctggccgcgc
     3001 tcgtgaccaa cctcgtcgag gccgatgtgc tggtgatact caccgaccag gccgggctgt
     3061 attcggccga tccgcgccac gatccgcagg cgaagctggt tccgtcggca atcgcgggtg
     3121 tgcctgaact cgaggcgatg gccggcgggg ccggtagcgc catcgcgcgc ggcggcatgc
     3181 tgacaaaagt tctcgccgca aagcgcgcgg cgcgcagcgg cgcgcacacg ctcattgcgt
     3241 cgggtcgcga gccggacatc ctgctgcgac tggcggctgg cgaggcgatt ggctccgaac
     3301 ttaaagcctt gacccccgtc ctgaccgccc gcaagcaatg gcttgcggac catctgttac
     3361 tgaagggccg cgtcgtcgtc gacgatggcg ccgcgaaggc gctcgcgtcg ggggggaaga
     3421 gcctgctgcc tatcggggtg attgaggttg agggcgactt ccagcgcgga gacgccgtcg
     3481 gctgcgttac gcgcgaaggg cgggagattg cacgcggcct cgcgaattac tcgagctcgg
     3541 acgcaaggca gatcgcacgc cggccgacga gtgacatcca gggcatcctc ggattcatcg
     3601 aggagcccga actgatccac cgcgataatc tcgttctgct ttgagcccgg gtctaggcgc
     3661 cttctccacc ggcccgagtg tcggaagcct caaagaccga gggcgcagtg cccgttcgtt
     3721 cgccgtccag ggcgacgtcg cgcgaccttg atcgcatcgc cttgtggtgc ggccgtccgg
     3781 gcttgaagac atagcgcgag agctcgagta gcgcctgctg atagacatca cgcttgaatt
     3841 cgatcacggt gtcgagcggc acccagtagt catgccagcg ccacgcatcg aactccgggt
     3901 gggtcgaggc acgcagacag acatcgcagt cgcgtccgac gaggcgaagc aggaaccaga
     3961 tctgtttctg cccgcggtag tttccacgaa gatcgcgtcg tatccactgg tcgggcacgt
     4021 catagcgcag ccagtcgcgt gtccggccga cgatcgtcac atgctccgtc ttcaggccaa
     4081 cttcttccca cagttcccgg tacatcgcct gctcgggcga ctcgccgtgc ttgatgccgc
     4141 cttgcggaaa ctgccatgaa tgctgtttga cgcgcttgcc ccagaagacc tcgtttcgac
     4201 cgttgagcag gatgatgccg acgttcgggc ggtacccgtc cttgtccagc atgccaaccc
     4261 ctgaagcttt agaatctgcg acccattata ggggtcgcat cctcgcgatc acaacgacga
     4321 aaagtcaccc ttttccatgc gcgccagaga cttcctgata tcgacactga aagaagcacc
     4381 ggccgatgcg gaggtggtga gccattcgct gatgttgcgc gccggcatga tcaagaagct
     4441 cgcagcgggt atctatacct acatgccgat gggcttgcgc gtcatccgca agatcgagtc
     4501 catcatccgc gaagaaatga accgtgcggg agcggtggag ctgctgatgc ctgttatcca
     4561 gcccgccgag ctttggatgg aatccggtcg ctggcacaaa atggggccgg agttgctgcg
     4621 cctgaaggac cggcacgagc gcgatttcat cgtgcagccg accagcgagg aagtcgtggt
     4681 cgacatcgtg cgtgacgaga tcaggagcta taagcagctc ccactcaatc tgtatcacat
     4741 tcagaccaaa ttccgcgatg agcgccgccc tcgcttcggt gtgatgcgtg gccgcgagtt
     4801 cacgatgaag gatgcctact cgttcgatcg ggacgaggac ggcgcgatga cgagctaccg
     4861 cgcgatgtac gacgcctacg tgcgcatctt ccagcgcatg gggcttcgat ttcgagcggt
     4921 cgcggcggac accggcaaca ttggcggcaa ggcgagccac gagttccagg tgattgccga
     4981 cacgggtgaa gacgccattg cctattgccc gacgtccgac tatgccgcga acgtcgagat
     5041 ggcggaggcg gtgccgctca cggcgcaccg cgtggcaccg acgcagccgc tgaccaagac
     5101 gccaaccccg ggtaaggaga agtgcgagga cgtagctgag ctgctgggcc taccgctgtc
     5161 gcagacagtg aaatcgatcg tcctggcagt cgagcggggc gacgccgaaa agccgacgga
     5221 acctgaagtc tggttggtga tgatccgtgg cgatcacgaa ctcaatgaga tcaaggtcag
     5281 taaggtcccg ggcctggacc agggcttccg cttcgccacc gaggcggaga tcgtggacca
     5341 tttcggctgc aggcccgggt a
//
LOCUS       DATIRT010000055         7960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_891391, whole genome shotgun sequence.
ACCESSION   DATIRT010000055 DATIRT010000000
VERSION     DATIRT010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7960
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891391"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..322
                     /gene="rpsJ"
                     /locus_tag="VLN25_01315"
     CDS             11..322
                     /gene="rpsJ"
                     /locus_tag="VLN25_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004199280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HSQ09243.1"
                     /translation="MQSQRIRIRLKAFDYKLIDQSALEIVDTAKRTGAVVRGPVPLPT
                     RIQRFDILRSPHVNKTSRDQFEMRTHQRLMDIIDQTDKTVDALMKLDLPAGVDVEIKV
                     Q"
     gene            492..1163
                     /gene="rplC"
                     /locus_tag="VLN25_01320"
     CDS             492..1163
                     /gene="rplC"
                     /locus_tag="VLN25_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HSQ09244.1"
                     /translation="MSEARSTLGLLGRKVGMTRIFTDDGESVPVTVLDVSNNRVSQVK
                     TAETDGYSAVQVAYGTRKSSRVAKPQAGHYAKSGVEAASVLREFRLDAAKVAEMKAGT
                     AIGVDTFSVGQKVDVTGVTIGKGFAGAIKRHHFSSNRATHGNSVSTNAPGSIGNRQDP
                     GRVFPGKRMAGHLGDEQRTTQNLVIARIDAERGLLLIKGAVPGSAGGNVIVRPAAKAK
                     AAAKA"
     gene            1176..1796
                     /gene="rplD"
                     /locus_tag="VLN25_01325"
     CDS             1176..1796
                     /gene="rplD"
                     /locus_tag="VLN25_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HSQ09245.1"
                     /translation="MELKLLNAQGQSDGTTSAPETLFGREFNEALVHQIVVAYQANAR
                     QGTRSQKDRTTVKHSTRKPWRQKGTGRARAGMTSSPIWRGGGRAFPNSPDENFSHKVN
                     KKMYRAGMTSILSKLARDGRIAVVDGLAVDSPKTKLLADKLKAMGLESVLIITDTVDE
                     NLYLASRNLKNVLIVEPRYADPLSLIHYKKVIVTKPAIAKLEEMWG"
     gene            1793..2104
                     /gene="rplW"
                     /locus_tag="VLN25_01330"
     CDS             1793..2104
                     /gene="rplW"
                     /locus_tag="VLN25_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008343846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HSQ09246.1"
                     /translation="MSTPTRQDRLYSVLLAPIVSEKSTMIGEKNDQVAFRVMQDATKK
                     EVKAAVEHLFKVQVDKVSILNQKGKSKRFGRFTGKRGNVRKAYVCLKEGQEINFAQEV
                     K"
     gene            2104..2928
                     /gene="rplB"
                     /locus_tag="VLN25_01335"
     CDS             2104..2928
                     /gene="rplB"
                     /locus_tag="VLN25_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HSQ09247.1"
                     /translation="MALVKVKPTSAGRRGLVKVVNENLHKGKPFAGLLEKKTRSSGRN
                     NAGRITTRHMGGGHKQHYRVIDFKRTKDGVPAKVERLEYDPNRSAHIALLVYGDGERR
                     YIIAPKGVAVGAQLMSGNEAPIRVGNTLPLRNIPVGTTVHCVEMLPGKGAQLARAAGT
                     SVQLLAREGTYAQLRLRSGEIRRVLIDCRATIGEVGNEENNLRQIGKAGAMRWRGVRP
                     TVRGVAMNPVDHPHGGGEGRTGTKRDPVSPWGQLAKGYRTRNNKRTDTMIVQRRRK"
     gene            2939..3223
                     /gene="rpsS"
                     /locus_tag="VLN25_01340"
     CDS             2939..3223
                     /gene="rpsS"
                     /locus_tag="VLN25_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HSQ09248.1"
                     /translation="MSRSLKKGPFVDGHLLKKVETAQAIRDKKPIKTWSRRSMILPEF
                     IGLTIAVHNGKQHVPVYINENMVGHKLGEFALTRTFKGHAADKKAVVAKK"
     gene            3234..3578
                     /gene="rplV"
                     /locus_tag="VLN25_01345"
     CDS             3234..3578
                     /gene="rplV"
                     /locus_tag="VLN25_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HSQ09249.1"
                     /translation="METTASVRGVRLSAQKGRLVADLIRGKPVDKALNILGFTQKKAA
                     GIIRKALQSAIANAEHNDGADIDELRVTGILVEKAATLRRFSARAKGRGARIGKQTCH
                     IFVTVGDAKPKK"
     gene            3586..4479
                     /gene="rpsC"
                     /locus_tag="VLN25_01350"
     CDS             3586..4479
                     /gene="rpsC"
                     /locus_tag="VLN25_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HSQ09250.1"
                     /translation="MGQKINPTGFRLPVTRNWGSRWFATGPAFPKMLLEDLKVREFLR
                     KKLRTASVGRILIERPAKNARITIYSARPGVVIGKKGEDIESLKATLQKMLGVPVHVN
                     IEEIRKPELDAQLIADSITQQLEKRIMFRRAMKRAMQNAMRLGAQGIKIMSAGRLNGI
                     EIARTEWYREGRVPLHTLRADIDYGFSEAKTTYGVIGVKCWVYKGDTLGRNEAPAATD
                     ATPAPDREDRRPRRGEGRPDSRPAGKAPAKRPRKTAEVKVEPTVPVVEKPKDDPAKPV
                     VNRVRKVAAKSPTEGGAKDGE"
     gene            4483..4899
                     /gene="rplP"
                     /locus_tag="VLN25_01355"
     CDS             4483..4899
                     /gene="rplP"
                     /locus_tag="VLN25_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HSQ09251.1"
                     /translation="MLQPARRKYRKEQKGRNRGLALRGAHVSFGEFGLKVTERGRLTA
                     RQIEAARRAISRHIKRGGRVWIRVFPDKPISQKPAEVRMGNGKGNPEYWVAEVRPGNV
                     VYELDGVTETLAREAFALAAAKLPLKTTFVVRQLGA"
     gene            4912..5106
                     /gene="rpmC"
                     /locus_tag="VLN25_01360"
     CDS             4912..5106
                     /gene="rpmC"
                     /locus_tag="VLN25_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HSQ09252.1"
                     /translation="MNVKEMRAKDADALTKELQELQKAHFSLRMQQATQQLQNTSQIR
                     NTRRDIARVRTVLAQKATAK"
     gene            5103..5372
                     /gene="rpsQ"
                     /locus_tag="VLN25_01365"
     CDS             5103..5372
                     /gene="rpsQ"
                     /locus_tag="VLN25_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HSQ09253.1"
                     /translation="MNAEKKSLTRTLVGKVVSNKMDKTVTVLVERRVKHPVYGKYVVK
                     SNKYHAHDETDALNEGDIVEISEGRPISRTKSWKVTRVVEAAQAI"
     gene            5640..6008
                     /gene="rplN"
                     /locus_tag="VLN25_01370"
     CDS             5640..6008
                     /gene="rplN"
                     /locus_tag="VLN25_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HSQ09254.1"
                     /translation="MIQMQTMLDVADNTGARSVMCIKVLGGSKRRYAHIGDVIKVTIK
                     ECAPRGRVKKGEVYSAVVVRTAKGLRRGDGSAIKFDNNAAVLLNAKLEPIGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            6022..6342
                     /gene="rplX"
                     /locus_tag="VLN25_01375"
     CDS             6022..6342
                     /gene="rplX"
                     /locus_tag="VLN25_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003806917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HSQ09255.1"
                     /translation="MERIRKGDEVIVIAGRDKGKRGVVLHRVDESHLVIEGINMVKKH
                     VRPNPMKGVAGGITDKTMPIDQSNVMLFNPSSSKGDRVGFKQVEGKKVRVFKSNGAVV
                     GAKQ"
     gene            6355..6894
                     /gene="rplE"
                     /locus_tag="VLN25_01380"
     CDS             6355..6894
                     /gene="rplE"
                     /locus_tag="VLN25_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HSQ09256.1"
                     /translation="MARLHTQYREKIVPELMTKFGYKTVMQVPRIAKITLNMGVGEAV
                     NDKKNIDMAVGDLTKIAGQKPVVTKARKAIANFKIREGLAIGTMVTLRGERMYEFLDR
                     LVTVALPRVRDFRGVSGRAFDGRGNYNIGLKEQIIFPEIEYDKIDKLRGLNISITTTA
                     KSDDEAKALLAAFRFPFRN"
     gene            6906..7211
                     /gene="rpsN"
                     /locus_tag="VLN25_01385"
     CDS             6906..7211
                     /gene="rpsN"
                     /locus_tag="VLN25_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HSQ09257.1"
                     /translation="MAKLSLINREAKREAAVKKHAARRAELKSIIDDQTRSMDERMDA
                     MKRLQALPRNSSPVRLRNRCALTGRPRGTFRKFGMARAKIREAAMRGDIPGLTKASW"
     gene            7225..7620
                     /gene="rpsH"
                     /locus_tag="VLN25_01390"
     CDS             7225..7620
                     /gene="rpsH"
                     /locus_tag="VLN25_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HSQ09258.1"
                     /translation="MSMSDPIADMLTRIRNAQRVEKPEVAMPSSKVKVAIAQVLKDEG
                     YIEGFQVNANDGKPELRIGLKYYAGRPVIESLERVSRPGLRVYKGSDDIPAVMNGLGV
                     AIVSTSRGVMTDRRARSQGVGGEVLCYVA"
     gene            7638..>7960
                     /gene="rplF"
                     /locus_tag="VLN25_01395"
     CDS             7638..>7960
                     /gene="rplF"
                     /locus_tag="VLN25_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HSQ09259.1"
                     /translation="MSRVARIPVTVAKGVDVQVTESNITVKGPLGTLHQALSGRVKVE
                     LANGEVSFVAADESREANAMSGTMRALVAAMVQGVSKGFEKKLSLVGVGYRSQAQGDK
                     LNLSLG"
BASE COUNT         1702 a         2394 c         2625 g         1239 t
ORIGIN      
        1 aggatctgac atgcaaagcc aacgtatacg catccgcctg aaggcgttcg actacaagct
       61 gatcgaccag tcggcgctcg agatcgtgga cactgcaaag cgcaccggtg cggtggttcg
      121 cggcccggtg ccgctgccga cccgcatcca gcgcttcgac atcctgcgtt cgccgcacgt
      181 caacaaaacg tcgcgcgatc agttcgaaat gcgcacccac cagcggctga tggacatcat
      241 cgaccagacc gacaagacgg tcgatgcgct gatgaagctc gacctgccgg ccggcgtgga
      301 cgtcgagatc aaggttcagt aaggctgctt gcctggaagg ctcagcccgg atagaatcgc
      361 cggctttccg cacccgggcg gattgaaacg atttcgcccc ggatgcttga acgcggagcg
      421 cgccgccaca ggtgaagcgc gctacgcgaa attttggtcc tggccaatcg aagccgggaa
      481 tggagataag aatgagcgaa gctcgctcga cgctcggctt gctgggccga aaagtcggca
      541 tgacgcgcat cttcaccgat gacggggaat ccgttccggt cacggtgctc gacgtgtcga
      601 ataaccgcgt cagccaggtc aagacggctg agaccgacgg ctattccgcg gtccaggttg
      661 cctacggcac ccgcaagtca tcccgtgtcg ccaagcccca ggcgggccac tatgccaagt
      721 ccggcgtcga ggcagcctcg gtccttcgcg agttccggct cgacgctgcc aaggtcgctg
      781 agatgaaggc gggtaccgcg atcggggtcg acacgttcag cgtcggccag aaggtcgatg
      841 tcaccggggt cacgatcggc aagggatttg ccggagcgat caagcgccac cacttctcgt
      901 cgaaccgcgc cacacacggc aattccgtgt cgaccaatgc gccaggctcg atcggcaacc
      961 ggcaggatcc gggccgggtg tttcctggca agcgcatggc gggccacctc ggcgacgaac
     1021 agcgcaccac ccagaatctc gtgatcgcgc gcattgatgc cgagcgcgga ctgctgctca
     1081 tcaagggcgc tgtccccggt tcggccggtg gcaacgtgat cgtgcgtccg gctgccaagg
     1141 ccaaagcagc ggccaaggcc taaggagagg gcgcaatgga actcaagcta ctgaacgccc
     1201 agggccagtc tgacggcacg acgtctgcgc cggagacgct tttcgggcgc gaattcaacg
     1261 aagcgctggt gcaccagatt gtcgttgcct accaggccaa cgcacgtcag ggcacgcgct
     1321 cgcagaagga ccggacgacg gtcaagcatt ccacgcgtaa gccctggcgc caaaagggca
     1381 ctggccgtgc acgcgccggg atgacgtcaa gcccgatctg gcgcggtggt ggccgcgcgt
     1441 tcccgaactc gcctgacgag aatttctcgc acaaggtgaa caagaagatg taccgcgcgg
     1501 gcatgacatc gatcctgtcg aagctggcgc gtgatggccg catcgccgtg gtcgatggtc
     1561 ttgcagtcga ttcgcccaag accaagctgc tggccgacaa gttgaaggcc atgggcctgg
     1621 agtcggtgct gatcatcacg gatacggtgg acgaaaacct gtacctcgcg tcgcgcaacc
     1681 tgaaaaacgt cctgatcgtc gaaccgcgct acgcggaccc gctgtcgctg atccactaca
     1741 agaaggtgat cgtgaccaag ccggcgatcg ccaagctcga ggagatgtgg ggatgagcac
     1801 gccgacccgt caggaccgtc tgtacagcgt gctgctcgca cccatcgttt ccgagaagag
     1861 cacgatgatc ggcgagaaga atgatcaggt cgcgttccgc gtcatgcagg acgccaccaa
     1921 gaaagaagtc aaggccgccg tcgagcacct tttcaaggtg caggtcgaca aggtttccat
     1981 cctgaaccag aagggcaaga gcaagcgctt tggccgtttc accggcaagc gcggcaacgt
     2041 gcgcaaggcg tacgtgtgcc tcaaagaagg tcaggaaatc aacttcgcgc aagaggtgaa
     2101 gtaatggcac tcgtcaaggt caaaccgacg tctgccggcc ggcgcgggct ggtcaaggtc
     2161 gtcaatgaaa acctgcacaa gggcaagccg ttcgcgggcc tgctcgagaa gaaaacgcgt
     2221 tcgtccggcc gcaacaatgc cggccggata acgacccgcc acatgggcgg tggccacaag
     2281 cagcactatc gggtcatcga cttcaagcgg accaaggacg gcgtgccggc caaggtcgag
     2341 cgcctcgagt acgacccgaa ccgttcggct catatcgctc tgctggttta tggcgacggc
     2401 gagcggcgct acatcattgc gcccaaaggc gtggcggtcg gcgcccagtt gatgagcgga
     2461 aacgaagcgc cgatccgtgt cggcaacacg ctgcccctgc gcaacatccc ggtcggcacg
     2521 accgtgcact gcgtcgagat gcttcctggc aagggtgcgc agctggcgcg tgccgccggc
     2581 acgtcggttc agctgcttgc gcgcgaaggc acctacgcgc agttgcggct gcgctccggc
     2641 gagatccgtc gagtgctgat cgattgccgc gcgacgattg gcgaggtcgg caacgaggag
     2701 aacaacctgc gccagatcgg caaggccggc gccatgcgct ggcgtggcgt gcgtccgacc
     2761 gtacgcggcg tggcgatgaa ccccgtcgat cacccgcacg gcggcggcga gggccgtacc
     2821 ggtaccaaac gcgatccggt cagcccgtgg ggtcagctgg ccaagggtta tcgaacccgc
     2881 aacaacaagc gcaccgacac gatgatcgtg cagcgccgtc gcaagtaagg ggtcctgaat
     2941 gtctcgttct ctgaaaaagg gcccgttcgt cgacgggcat ctgctgaaga aagtcgaaac
     3001 ggcgcaggcc atccgtgaca agaagccgat caagacctgg tcgcgtcgct cgatgatcct
     3061 gccggagttc atcggcctga cgatcgccgt gcataacggc aagcagcacg tgccggtcta
     3121 tatcaacgaa aacatggtcg gtcacaaact cggcgagttt gcgctgaccc ggaccttcaa
     3181 gggtcacgcg gcggacaaga aggccgtcgt ggcgaagaag taaggtgatg acgatggaaa
     3241 cgactgcatc ggtgcgcgga gtacgactgt cggcccagaa gggtcggctt gttgccgacc
     3301 tgattcgcgg caagccggtc gacaaggcgc tgaacatcct gggcttcacg cagaagaagg
     3361 cagccgggat catcaggaag gcgcttcagt cggcgattgc caacgccgag cacaacgatg
     3421 gcgctgacat cgacgagctg cgggtcaccg gcatcctggt ggagaaagca gcaacgctgc
     3481 gccggttttc ggcgcgggcc aaggggcgcg gtgcgcgcat cggcaaacag acctgccaca
     3541 tctttgtgac ggtcggcgac gccaaaccca agaaataggt gatccatggg tcaaaagatc
     3601 aatcccaccg gcttccggct ccccgttacg cgtaactggg gctcgcgctg gtttgccacc
     3661 ggtccggcgt tccccaagat gctgctcgag gacctcaagg tccgcgagtt tctgcgcaag
     3721 aagctgcgca ccgcttcggt cggccgcatc ctgatcgagc gtccggccaa gaacgcgcgt
     3781 atcacgatct actcggcgcg accgggggtg gtcattggca agaagggcga ggatatcgag
     3841 tcgctcaagg cgacgctgca gaaaatgctc ggcgtgccgg tgcacgtcaa tatcgaggaa
     3901 atacgcaagc cggagctgga cgcccagctg atcgccgatt cgatcaccca gcagctcgag
     3961 aagcgcatca tgttccgccg cgcgatgaag cgcgcgatgc agaacgcgat gcggctcggc
     4021 gcccaaggca tcaagatcat gagcgcagga cgcctgaacg gcatcgaaat cgcccggacc
     4081 gagtggtatc gcgaaggtcg tgttccgctg catacgttgc gtgcggacat cgactacggt
     4141 ttctcggaag ccaagaccac gtacggcgtc atcggcgtca agtgctgggt ttacaagggc
     4201 gacacgctgg gtcgcaacga agcgcccgcg gcgaccgacg ccacgcccgc accggatcgc
     4261 gaggaccgtc gtccgcgccg cggcgaaggt cgtcccgaca gtcgtccggc aggcaaggcg
     4321 ccggccaagc ggccgcgcaa gaccgctgaa gtcaaggtcg agcccacggt gccggtcgtc
     4381 gagaagccca aggacgaccc ggccaagccg gtcgtcaatc gtgtgcgcaa ggtggcggcc
     4441 aaatcgccga ccgagggcgg cgcgaaagac ggagagtaag ccatgctgca acctgctcgt
     4501 cgcaagtacc gcaaggagca aaagggccgt aaccgcgggc tcgcgctgcg cggcgcccat
     4561 gtgtcctttg gcgagttcgg actgaaggtc accgagcgcg gccgactgac tgcacgccag
     4621 atcgaagcgg cgcgccgtgc gatcagccgc catatcaagc gcggtggccg tgtctggatc
     4681 cgcgtgttcc cggataagcc gatttcgcag aagccggccg aggttcgcat gggcaatggc
     4741 aagggcaacc ccgagtactg ggtcgccgaa gtgcggcctg gcaatgtggt ttatgaactc
     4801 gacggcgtga cggaaacgct ggcgcgcgaa gcgtttgccc tcgccgcggc caagctgccg
     4861 ctaaagacga cgtttgtcgt ccgtcagctg ggcgcctaag gagaccggac gatgaacgtc
     4921 aaggaaatgc gcgcgaagga cgctgacgcg ctcaccaagg agctgcagga actgcagaaa
     4981 gcgcattttt ccctgcgcat gcagcaggcc acgcagcaat tgcaaaatac gagccagatc
     5041 cgcaacaccc ggcgcgacat cgcgcgggtg cggaccgtgc tggcgcagaa ggcgaccgcc
     5101 aaatgaacgc tgaaaagaaa tccctgacgc gcacgctggt gggcaaggtt gtcagcaaca
     5161 agatggataa gacggtgacc gtcctggtcg aacggcgggt caagcacccc gtctacggca
     5221 agtacgtcgt caagtcgaac aagtatcacg cgcacgacga gaccgatgcg ctgaacgaag
     5281 gcgacatcgt cgagatctcc gagggccgtc cgatttccag gaccaagagc tggaaggtca
     5341 cgcgcgtggt tgaggcggcg caggctatct agcgcttgtc gcgttgcggc aaaagcggat
     5401 atactcgctg gctttccgcc gacagggtcg atacctaccg acggatggcc tgcagggcag
     5461 taggcaagtg tagtcagtga gcagttttcc ggagcgcgca ggtgcgcttc gtccccccgc
     5521 cggcggcggg gcacaggacg gacgcacggc gggctcctca acccggacgg gaccaagact
     5581 ggctgttgcg tcgagcctcc aaggctcggt ccgatagttc aagttgggat cgaaaagcca
     5641 tgattcagat gcagacgatg ctcgacgtcg ccgacaacac cggcgcacgt tcggtgatgt
     5701 gtatcaaggt gttgggcggc tcaaagcgcc gctacgcgca catcggtgac gtcatcaagg
     5761 tgacgatcaa ggaatgcgcg ccgcgcggtc gcgtcaaaaa gggcgaggtc tacagcgcgg
     5821 tcgtcgtgcg cacggccaag ggcctgcgac gcggggacgg ctcggcgatc aagttcgaca
     5881 acaacgcggc ggtactactc aacgccaagc tggagcccat cggcacccgc atcttcggcc
     5941 cggtcacgcg cgagctgcgt accgagcgct ttatgaagat cgtgtcgctg gcaccggaag
     6001 ttctgtaggg ggagcgcgga catggaacgt atccgcaagg gtgacgaggt catcgtcatc
     6061 gcaggcaggg acaagggtaa gcgcggcgtc gtgctgcacc gggtcgatga gtcgcacctc
     6121 gttatcgagg gcatcaacat ggtgaagaag catgtccggc cgaacccgat gaagggtgtg
     6181 gccggcggca tcactgacaa gacgatgccg atcgatcagt cgaacgtgat gctgttcaat
     6241 ccgtcgagca gcaaaggcga ccgagtgggc ttcaagcagg tcgagggcaa gaaagtgcgc
     6301 gttttcaaga gcaacggcgc cgtcgtcggc gcgaagcagt aaggggaact ggaaatggcg
     6361 cgtttgcaca cccagtaccg cgagaagatc gtgcccgaac tgatgaccaa gttcggctac
     6421 aagaccgtga tgcaggttcc gcgcatcgcg aagatcacgc tgaacatggg cgtcggtgag
     6481 gcggtcaacg acaagaagaa catcgacatg gcggttggcg acctgacgaa gatcgccggg
     6541 cagaagccgg tcgtgaccaa ggcccgcaag gcgatcgcga acttcaagat ccgcgaaggc
     6601 ctggcaatcg gcaccatggt gaccctgcgg ggcgagcgga tgtacgagtt cctcgaccga
     6661 ctggtgacgg tcgccctgcc gcgcgtgcgg gacttccgcg gcgtgtcggg ccgcgccttc
     6721 gatggccggg gcaactacaa catcggcctg aaggaacaga tcatcttccc ggagatcgag
     6781 tacgacaaga tcgacaagct ccggggcctg aacatcagca tcacgacgac cgcgaaatcg
     6841 gacgatgaag ccaaagcgct tctcgccgcg ttccggttcc cgttccgcaa ttaagcgagg
     6901 ggaaaatggc gaagctctca ttgataaacc gcgaagccaa gcgcgaggcg gcggtcaaga
     6961 agcacgctgc caggcgcgct gaactgaagt ccatcatcga cgaccagacg cgttcgatgg
     7021 acgagcgcat ggacgcaatg aagcggctcc aggcgctgcc gcgcaattcc agccccgtac
     7081 ggctgcgcaa ccgttgcgcg ctgaccggcc gtccgcgtgg cacgttccgc aagttcggta
     7141 tggcgcgcgc aaagatccgc gaagcagcga tgcgcggcga tatcccgggc ctgaccaagg
     7201 ccagctggta agcgaggaac gcacatgagc atgagtgatc ccatcgccga catgctgacc
     7261 cgcatccgca atgcacagcg ggtcgagaag ccggaagtcg cgatgccgtc gtcgaaagtc
     7321 aaggtggcca ttgcccaggt cctgaaggac gagggataca tcgagggctt ccaagtgaat
     7381 gccaacgatg gcaagccgga attgcgcatc ggcctgaagt actacgccgg ccgtccggtc
     7441 atcgagagtc tcgagcgcgt atcgcgtccg ggtctgcgcg tttacaaggg cagcgacgac
     7501 attcccgccg tgatgaacgg actgggcgtg gcgattgtct cgacctcgcg cggtgtgatg
     7561 accgaccgca gggcccgcag ccagggcgtc ggcggcgaag tgctctgcta cgtcgcgtaa
     7621 cggagcggag gaagaccatg tctcgagtag cacgaattcc ggtgaccgtc gccaagggcg
     7681 tcgacgttca ggtcacggaa agcaacatca cggtaaaagg gccgctgggc acgctgcacc
     7741 aggcgttgtc gggccgcgtg aaggtcgagc tggccaacgg cgaagtctca ttcgttgcgg
     7801 ccgacgagtc acgtgaggcc aacgcaatga gcggcaccat gcgcgcgctg gtggcggcca
     7861 tggtgcaggg tgtttcgaag ggcttcgaga agaagctgtc tctggtcggc gtcggttatc
     7921 gctcccaggc gcagggcgac aagctgaacc tttcgctggg
//
LOCUS       DATIRT010000056         1803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1481451, whole genome shotgun sequence.
ACCESSION   DATIRT010000056 DATIRT010000000
VERSION     DATIRT010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481451"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..1519
                     /locus_tag="VLN25_01400"
     CDS             275..1519
                     /locus_tag="VLN25_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HSQ09260.1"
                     /translation="MTLVPSLEAAWRDVDSSFERFCLTAGIGAIEQMLCEDAQQLAGP
                     RHSRGERVGHRWGATKGKLGFHGGKVVVSRPRVRSEGQEVALPTWKAAQAEDWLGRWA
                     MNLMLINVSTRKLKRAVRLPEGDLPAVMGDGTSKSAASRRFVALSAERMAEWMASDLS
                     KLDLLVIQIDGLHVGNDLVLVAALGIDSEGHKHPLGLIEGATENATVVQALMDNLIER
                     GVDPTICRLFIVDGAKALTKVIRSTFGRHTPIQRCQVHKARNVVDRLPKRLHASARKA
                     LRQAWQFDDADKAERLLRNLARRLEQEAPGVAASILEGLDEILTVNRLGLPAQLRRSL
                     ACTNSIENVMGTVRRVCRNVKRWRNAAMALRWTAAGMLEAAKGFRRLKAHKQLPILRA
                     ALAAHHAKHTTKEKLEDGLKAA"
BASE COUNT          374 a          544 c          559 g          326 t
ORIGIN      
        1 tcgatcaatc cgcgcacgcg ctggcgaccg cgctcgagcg cggccacgag cagggactgg
       61 cccgcgacat tcaggagcgt gtcgtgcaga tcttcgaggt ctgcaatttc caggatctga
      121 ccggccagcg cgtgtccaag gcggtgacga cgggaatgtc ccgatcgatg ttgaattagg
      181 agagcaggcg ttgcccgcct tgagccgtta ggctcggggt gtcgaatccg cgaaggagag
      241 caacgccatg acgaatccta ccacgacttc accgatgaca ctggtgccga gccttgaggc
      301 ggcttggcga gatgtggaca gctcattcga gcgcttctgt ctgacggcgg gcatcggagc
      361 gattgagcag atgctgtgcg aggacgcgca gcaactggcc ggtccccgcc atagccgtgg
      421 cgaacgcgtt ggccaccgct ggggtgcgac caaggggaag ctcggcttcc atggcggcaa
      481 ggttgttgtt agccggccac gggtgcgcag cgagggccag gaggttgcgc taccgacctg
      541 gaaggcggcg caggcggagg attggcttgg acgctgggcg atgaacctga tgctgatcaa
      601 cgtatcgacg cgcaaactta aacgcgcggt acggctgccc gagggcgatc tgccggcagt
      661 catgggtgac ggcacgtcaa aatcggcggc ttcgcggcgg ttcgtagcac tatctgccga
      721 gcgcatggcc gaatggatgg cttcggacct gtccaagctg gacctgctgg tcatccagat
      781 cgatggcctg cacgtcggca acgacctggt cctggtagcc gcgctcggta tcgacagcga
      841 gggccataag catccgcttg gcctgatcga aggcgctact gagaatgcca ctgtcgtgca
      901 ggctctcatg gacaatctga tcgaacgtgg ggtcgatccc acaatctgtc gattgttcat
      961 cgtcgacggc gcgaaggcac tgaccaaggt gatccgctcg acctttggtc ggcacacgcc
     1021 gatccaacgc tgccaggttc acaaagcccg caatgtggtc gatcgcctgc ccaaacggct
     1081 gcatgcctcc gcgcgcaagg cgctgcgaca agcctggcag ttcgacgatg ccgacaaggc
     1141 cgagcggctg ttgcgcaatc tcgcccgcag gctcgagcaa gaagcgccag gcgttgctgc
     1201 gagcatcctc gaagggctcg acgagatact gaccgtcaac cgccttggtc taccagcgca
     1261 gctccgacgc tcgcttgcct gcaccaactc aatcgagaac gtgatgggga cagtgcgtcg
     1321 cgtctgtcgc aatgtgaagc gatggcgaaa cgcagccatg gcgctgcgct ggaccgccgc
     1381 cggcatgctg gaggccgcca agggcttccg acgtctcaaa gcgcacaagc agctgccgat
     1441 actcagagcc gcgctagccg cgcatcacgc caagcatacg accaaggaga aacttgagga
     1501 cggtctgaaa gccgcatagc atcatcaaca ggcagcgctc gtcccgccga gttcaacaat
     1561 ggacgggaca tccccatgtg cccccctgcg gtctattaca cgccaagctt ttcgacgcga
     1621 atttgcttcc cgcactcgct gaagagtaat tcgtaatgaa ttccaccctt gtcaaacttc
     1681 tggaaagaga aataagcatt ccagcagttt tggtcccgaa cgaaatagtc gcccttactt
     1741 acgacgcagc agttcctctc gatgagcctg tcaatctgtg ccgcgtcatc aatatcaaag
     1801 ctg
//
LOCUS       DATIRT010000057         4896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_6801, whole genome shotgun sequence.
ACCESSION   DATIRT010000057 DATIRT010000000
VERSION     DATIRT010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4896
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6801"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VLN25_01405"
     CDS             <1..529
                     /locus_tag="VLN25_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HSQ09261.1"
                     /translation="GVICPMVNTRDDAARLVVYTSYAPRGTRSFGPMRALLYAGADYP
                     QHADYTIVRFAMIETAQALDNLDAILSVEGLDAIYVGPSDLSLSLGCKPVLDDVEASV
                     ASAIEHILARALAHGVLAGIHNGQVTTARQRIAKGFRFVSIASDAHLMASGARDTLTH
                     VRQGAPDEQHHGVIR"
     gene            complement(603..1070)
                     /locus_tag="VLN25_01410"
     CDS             complement(603..1070)
                     /locus_tag="VLN25_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HSQ09262.1"
                     /translation="MRLLYTGLLFVHLIGVVLWVGGMFVLHFAVRPAAAGQLAPPQRL
                     PLLANVLGRFFFWVSIAIVAILASGIGLILGAGGFANAHASVHLMFVLGLVMMAIFLV
                     IRFAPFPRLQRAVAASDWPAAARELDRVRKLVVTNLALGVITTAVATLGRALL"
     gene            complement(1121..3454)
                     /gene="parC"
                     /locus_tag="VLN25_01415"
     CDS             complement(1121..3454)
                     /gene="parC"
                     /locus_tag="VLN25_01415"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HSQ09263.1"
                     /translation="MLEQQTLFPASGGGDGDGSDALTLADFAQRAYLDYAISVVKGRA
                     LPDGADGQKPVQRRILFAMNEMGLASGARPVKSARVVGDVLGRFHPHGDQAAYDALVR
                     MAQNFTLRYPLIDGQGNFGSRDGDGAAAMRYTEARLTPIARLLLDEIDEGTVDFMPNY
                     DGSTQEPLLLPARLPFVLLNGASGIAVGMATEVPSHTLREVAAATLLLLANPKAKLDD
                     VLAKLPGPDFPGGGQIISPAGDIRDIYTSGRGSLKVRARCEFEELARGQWQLVVRELP
                     PSTSAQRVLEEIEELTNPKVKAGKKALTSDQQQTKQLMLSVLDTVRDESGRDAAVRLV
                     FEPRTSKIDRDEFVRTLLAQTSMETSAPINLVMIGRDGRPRCKPVVEILSEWIEFRVD
                     TVRRRSQHRLQKVEDRIHILEGRQAVLLNIDKVIKLIRGSDEPKPDLMKAFKLTERQA
                     EDILEIRLRQLARLEGIKIEQELAELRKNEGGLRKLLGADAALRKQVAREIDDDAKKY
                     GDGRRTLIETAERAAVEVKVIDEPMTVIVSEKGFVRARTGHGHDATQFSFRTGDALYG
                     AFECRSVDQLIAVGSNGRVYSVPVAQLPSARGDGAPVTSMIELEAGSRLVGYVAGGVE
                     TPLLLATTNGYGFAAALGDMLSRQKAGKQFITVDDDAEPLRPQLVKAESDRSIACLSG
                     KGRVLVFDAAEIKALSGGGRGVTLIDLDEGEKLLMALPIGSGAVMRATRGSAGKPVEV
                     RLVGAQLQAQHGHRARKGKLVDSKLKPPFALLRLPEA"
     gene            complement(3542..4411)
                     /locus_tag="VLN25_01420"
     CDS             complement(3542..4411)
                     /locus_tag="VLN25_01420"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HSQ09264.1"
                     /translation="MADALGQSEHVLHTTYFNGAWSDGNVPLIGAMDHSVWLGSSIFD
                     GARSIHRMAPDLRAHCERTYESAARMGLKLDLPIDELMRLCVEGIHRFPSDADLYIRP
                     LVFATAGLLVPEQCAFALTLFDAPLPPFEGFSAHLSQRRRPSPLSAVTDAKASALYPN
                     VARALREAAAAGYQNAVVLDPDGNVAEFATANLFFVAADGDVVTPAANGTFLAGITRA
                     RVLRLLAAEGIEVRQRTVRPQELDAAREIFNSGNYGKVMPCTRYGDRELAIGPVATLA
                     RQRYLEFAATCRV"
     gene            complement(4396..>4896)
                     /locus_tag="VLN25_01425"
     CDS             complement(4396..>4896)
                     /locus_tag="VLN25_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSQ09265.1"
                     /translation="QLRQLGCVEGLLDILNAYEVSPSEIEVEVTETGLLDTSAVSREN
                     LVRMRNAGVRLALDDFGVGFSSLAHLRDLPIHRLKIDRSFTVECMRDARTLTIVKAVI
                     EMARSLGISVTAEGIETQAQQTWMQHLGCDAAQGYLFARPMPGDDFIKLFIDRRGVGR
                     ERSLMH"
BASE COUNT          842 a         1657 c         1561 g          836 t
ORIGIN      
        1 cggtgtgatc tgcccgatgg tcaacacgcg cgacgacgcg gcccgcctgg tggtgtacac
       61 gagctacgca ccgcggggca cccgcagctt cgggcccatg cgtgcgctgc tctacgccgg
      121 tgccgactat ccgcagcacg ccgactacac catcgtgcgt ttcgcgatga tcgagacggc
      181 ccaggccctc gacaatctcg atgcgatcct atcggtcgaa ggactggatg ccatttacgt
      241 cggcccgtcc gacctttcac tgtcgctggg gtgcaagccg gtactcgacg acgtcgaagc
      301 ctcggtcgcc tccgcgatcg agcatatcct cgcccgcgct ctggcccatg gcgtcctcgc
      361 cggcatccac aatggccaag tcaccacggc tcgccagcgc atcgcaaagg gcttccgctt
      421 cgtatcgatc gcttccgatg cgcatctgat ggcttcgggg gcccgggaca cgctgacaca
      481 tgtgcgccag ggggctcccg acgagcagca ccacggagtc atacgttgac aagctatgac
      541 tactgcaccg cgatgtcttc acggaccgcg tcgtgacggt ctgccgcttc caggcgtgag
      601 cgctacagca gcgcgcgccc tagtgtggct actgccgtcg tgatcacgcc cagcgccagg
      661 ttggtcacca cgagtttgcg gacccggtca agttccctgg cagccgcggg ccaatcggag
      721 gcagcgaccg cccgctgaag tcgcggaaac ggcgcgaagc ggatgaccag gaagatcgcc
      781 atcatcacca gcccgagcac gaacatcagg tgcaccgagg catgtgcatt ggcgaagccg
      841 ccggctccga ggatcaggcc gataccgctg gctaggatgg cgacgatcgc gattgagacc
      901 cagaagaaga atcgccccag cacgttcgcc agcagcggca gccgctgtgg cggcgccagt
      961 tggccggccg cggccggccg caccgcaaag tgcagaacga acatcccgcc gacccacagc
     1021 acgacgccga tcaggtggac gaacagcagc cccgtgtaca ggagtctcat gcgggccttt
     1081 ctgaccggcg tgtcaaggca ggatgccacg tgttcattgc tcaggcttcc ggcagccgca
     1141 gcagcgcgaa cggcggtttc agtttggagt cgacgagctt gcccttgcga gcacgatggc
     1201 catgctgagc ctggagctgc gctccgacca gtcggacctc caccggcttg ccggccgagc
     1261 cacgggtcgc ccgcatgacc gctccgctgc cgatcggcaa tgccatcagc agcttttcgc
     1321 cttcgtccag atcaatcagc gtcacgcccc gtcctccgcc cgacagtgcc ttgatctcgg
     1381 cagcgtcgaa caccagtacc cgtcccttgc ccgagaggca ggcaatcgat cgatcgctct
     1441 cggccttcac gagctgcggc cgcagcggct cggcgtcgtc gtcgacggtg atgaactgct
     1501 tgcccgcctt ctgccgggac agcatgtccc caagcgcagc ggcgaaacca tagccattgg
     1561 tggtggccag cagcagcggc gtctcgactc cgccagcgac atagccgacc aggcgcgagc
     1621 cggcctcgag ctcgatcatc gaggtgaccg gggcaccgtc tccgcgcgcc gacggcaatt
     1681 gcgccaccgg caccgaatag acgcgaccat tgctgccgac cgcgatcagc tggtcgacgc
     1741 tgcggcactc gaaggcgcca tacagggcat cgccggtcct gaacgagaac tgggtcgcgt
     1801 catgaccatg tccggtgcgc gcgcgcacga accccttctc cgaaacgatg acggtcattg
     1861 gctcatcaat caccttgacc tcgacggcgg cgcgctcggc ggtctcgatc agcgtacgcc
     1921 ggccatcgcc gtactttttc gcatcgtcgt cgatctcgcg cgcgacctgc ttgcgcaatg
     1981 cggcgtcggc accgagcaac ttgcgcagcc ctccctcgtt cttgcgcagc tcggccagct
     2041 cctgctcgat cttgattcct tcgagtcgtg cgagctggcg cagacggatc tccaggatgt
     2101 cttcggcctg ccgttccgtg agcttgaacg ccttcatcag atccggcttg ggctcgtcgg
     2161 acccacggat cagcttgatg accttatcga tgttgagcag gactgcctgg cgcccctcca
     2221 ggatgtgaat ccggtcctcg accttctgca ggcgatgctg cgagcgtcgc cgcaccgtat
     2281 cgacgcggaa ttcgatccac tcggacagga tctcgacgac cggcttgcag cgtggccggc
     2341 catcccggcc gatcatgacg aggttgatcg gcgcgctggt ttccatgctg gtctgcgcca
     2401 gcagcgttcg cacgaattcg tcgcgatcga tcttcgaggt gcggggctcg aagaccaggc
     2461 gcacggctgc atccctgccg gactcgtcgc gcacggtgtc gagcaccgac agcatgagtt
     2521 gcttggtctg ctgctgatcg gaggtcagtg ccttcttgcc ggccttgacc ttcggattcg
     2581 tcagttcctc gatttcctcg agcacgcgct gggccgaggt ggacgggggc agctcacgga
     2641 caaccagctg ccactgcccg cgcgccagtt cctcgaattc gcagcgtgcc cgcaccttca
     2701 ggctgccacg gccgcttgtg tagatatccc ggatgtcgcc cgccggcgaa atgatctgtc
     2761 cgccgccggg aaagtcgggg cccggcagct tcgcgagcac gtcgtccagc ttcgccttcg
     2821 gattggccag cagcagcagt gtcgcggccg cgacttcacg cagggtatgc gagggcacct
     2881 cggtcgccat tccgaccgcg atgcccgacg cgccgttgag cagcacgaag ggcagtcgcg
     2941 ccggcaaaag cagcggctcc tgcgtcgaac cgtcatagtt cggcatgaaa tcgaccgtgc
     3001 cttcgtcgat ctcgtcgagc agcaggcgcg cgatcggtgt gagccgcgcc tcggtgtagc
     3061 gcatcgcggc tgcgccatcg ccatcacggc tgccgaagtt gccctgcccg tcgatcagcg
     3121 gataccgcag cgtgaagttc tgcgccatgc gcaccagcgc gtcatacgcc gcctggtcgc
     3181 cgtgaggatg gaatcgaccg agcacgtcgc cgacgacgcg cgccgatttc accgggcgcg
     3241 cccccgaggc caggcccatc tcgttcatcg cgaagagaat gcggcgctgg accggcttct
     3301 ggccgtccgc accatcaggc agtgcgcgcc ccttcacgac cgagatcgcg tagtcgaggt
     3361 aagcgcgctg cgcgaaatcg gccagcgtca gcgcatcgct gccatcgccg tcgcctccac
     3421 ccgaggcggg gaaaagtgtc tgttgctcaa gcatggttga acgtggtgcc cctcgcgcag
     3481 ttcaggcgat tcaggattct acggattgag cccgacgggc gtcgccgagg gtcagccggc
     3541 gtcacacacg acaggtggct gcgaactcca ggtagcgctg gcgtgccagc gtggcaacag
     3601 gaccgatcgc gagttcgcgg tcgccgtagc gtgtgcaagg catcaccttg ccgtagttgc
     3661 cggagttgaa gatctcgcgt gcggcgtcga gttcctgcgg cctgacggtg cgctgacgca
     3721 cctcgatgcc ctcggcggca agcagccgca gcacacgtgc gcgggtgatg cctgcgagaa
     3781 aggttccgtt cgcggccggc gtcaccacgt ctccgtccgc tgcaacgaag aacaggttgg
     3841 ctgtcgcaaa ttcggccacg ttgccgtcgg gatcaagcac gacggcgttc tggtagccgg
     3901 ccgcggcggc ctcgcgcaat gcgcgagcca cattcggata aagcgccgac gcttttgcat
     3961 ccgtgaccgc cgagagcggc gacggccggc gtcgctgcga caggtgtgcg gagaagccct
     4021 cgaatggcgg cagcggcgcg tcaaacagcg tcaacgcaaa cgcacactgc tccgggacca
     4081 gcaggccggc cgtggcgaac acgagcggcc ggatgtaaag atcggcgtcc gaggggaaac
     4141 ggtggatgcc ttcgacgcac agtcgcatca actcgtcgat cggcaagtcg agcttgagtc
     4201 ccatgcgcgc ggcggactcg taggtccgct cgcagtgggc gcgcaggtcc ggagccatgc
     4261 gatgaatcga acgcgcgccg tcgaagatcg acgagccgag ccagacgctg tggtccattg
     4321 cgccgatcag cggtacgttg ccgtcgctcc aggcgccatt gaagtaggtg gtatgcagaa
     4381 cgtgctccga ctgtcctagt gcatcagcga tcgctcgcga ccgacgccgc ggcggtcgat
     4441 gaacagcttg atgaagtcat cgcccggcat cgggcgtgcg aaaagatagc cctgcgccgc
     4501 gtcgcaaccc aggtgctgca tccatgtctg ctgcgcctgg gtttcgatgc cctcggctgt
     4561 gacgctgata ccgagcgagc gcgccatctc gatcaccgcc ttgacgatcg tcagcgtgcg
     4621 cgcgtcgcgc atgcactcga ccgtgaagct gcggtcgatc ttcaggcggt ggatcggcag
     4681 gtcgcgcagg tgcgccaggc tggaaaagcc gacgccgaag tcgtcgagcg caagccgcac
     4741 gccggcattg cgcatgcgca ccaggttctc gcgcgacacc gccgaggtgt cgagcaggcc
     4801 ggtctcggtg acctcgacct cgatctccga cggcgacact tcatacgcgt tgagtatatc
     4861 gagcagtccc tcgacacaac cgagttgccg caactg
//
LOCUS       DATIRT010000058         4265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_498722, whole genome shotgun sequence.
ACCESSION   DATIRT010000058 DATIRT010000000
VERSION     DATIRT010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498722"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1729)
                     /gene="mdoH"
                     /locus_tag="VLN25_01430"
     CDS             complement(<1..1729)
                     /gene="mdoH"
                     /locus_tag="VLN25_01430"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HSQ09266.1"
                     /translation="MELHHSGDLTAAVPGTTPPVARTPMAARPWHALRPTDAAPRNVP
                     GFSALEVSAARRRFVLLVLSLVPALYATALMSDLLPTTVDGWLQGTLLALFALLSCWV
                     AAGFWTAMAGLWILMRGGDEFLISRSVARAGPLPGDARTAIVMPICNENVHHVFAGLR
                     ATYESLARTGELERFDFFVLSDSSDPDTCVAELDAWGGLRETLGAEDRLFYRRRSRRV
                     KRKSGNIDDFCRRWGGNYRYMIVLDADSVMTGECLVTLARLMEARPDAGIIQTAPQAV
                     GRTTLHARAQQFASQVYGPLFTAGLHYWQLGESHYWGHNAIIRVAPFMRHCALAPLPG
                     HGALSGEILSHDFVEAALMRRAGFGVWIAYDLDGSYEQTPPNLLDEVKRDRRWCQCNL
                     MNARLMFVRGLHPVHRTVLFTGALAYVSAPLWLSFLVLSTWLLVQHGAADPQYFVMPH
                     QLYPLWPSWRPEHALGLAVAVATLLFLPKLVAALLAARRGAAQYGGASALAMTTLVEI
                     VLSALLAPVRMLFHSQFVLAAICGWSIQWNSPERADASTSWSEALQRHGVQSALGLAW
                     VALVAFNAPD"
     gene            complement(1702..3261)
                     /locus_tag="VLN25_01435"
     CDS             complement(1702..3261)
                     /locus_tag="VLN25_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0009250 - glucan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein G"
                     /protein_id="HSQ09267.1"
                     /translation="MTLNDSLSASGLLASVGLLLRAATVAALVLVGPAAQAQGFDLDS
                     VDKLARALAAKPFNASAPPLPKALKDLNYDQMRDIRFDPARSLWRAEKLPFELQFFHL
                     GGQFHTNPVRIHEVVGGTVREIPFDPADFDYGKNGLDRAQLRKLGFAGFRVHTPLNTQ
                     RYKDELLVFLGASYFRALGQGQRFGASARGLAVDTAERGGEEFPRFEQFWIVRPARNA
                     RELTVYALLDSRRVAGAYRFIVRPGAETVTEVTARLHLREAGAKIGLAPLTSMFFHGE
                     NQRASAEDFRPEVHDSDDLAIHAGNGEWNWRPLVNQRRLLVTSFAVTDPRGFGLMQRD
                     RAFDNFEDLEARYELRPSVWVEPVGSWGAGRVELVQIPTPDETNDNIVAYWVPQQTLR
                     PGQPFEFRYRLMWQKGTERRPPNAWVVQTRRGQGYAALPEDVIKLTVDFDGPALRRLP
                     ADAAVLGDLSVSGGQRLALNVHRNEVSGGWRMVVQVRRTEKDKPIELRAVLRNADSPL
                     SETWNYIIPAI"
     gene            complement(3398..3787)
                     /locus_tag="VLN25_01440"
     CDS             complement(3398..3787)
                     /locus_tag="VLN25_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09268.1"
                     /translation="MKRSLLAILLTTAVTAALAADVGISISVGQPGFYGQIDIGNAPR
                     PVLIYPQPVVIQRVQVVEPVQPIYLHVPPGHAKNWKKHCQKYDACARPVYFVKDDWYS
                     NVYVPHYEQEHGKGKGKAKGNGKGKEK"
BASE COUNT          800 a         1409 c         1359 g          697 t
ORIGIN      
        1 agtccggcgc gttgaaggcg accagagcta cccacgccag ccccagcgca gactgcacgc
       61 catgccgctg cagggcttcc gaccacgacg tactcgcatc ggcgcgttcc ggtgagttcc
      121 actggatcga ccagccacag attgcggcga gcacgaactg cgaatggaac agcatgcgca
      181 ccggcgcgag cagcgccgac agaacgattt cgaccaacgt ggtcatcgcc agcgcgctgg
      241 cgccgccgta ctgcgctgcc ccccgccggg ccgcaagcag cgcggcgacc agcttcggca
      301 gaaagagcag ggtcgcgacc gcgacggcca ggcccagagc gtgttccggt cgccacgaag
      361 gccacagcgg atacaactga tgaggcatga cgaagtactg cggatcggcc gcaccgtgct
      421 gcaccagcaa ccacgtcgac agcacaagga agctcagcca cagcggtgcc gagacgtaag
      481 cgagcgcacc ggtgaacaag accgtgcgat gaacgggatg cagaccgcgg acgaacatca
      541 aacgggcgtt catcagattg cactggcacc agcggcggtc acgcttgact tcgtccagca
      601 ggttcggcgg cgtctgttcg tagctgccat cgaggtcgta ggcgatccac acgccgaagc
      661 ctgcgcgccg catcagcgcc gcctcgacga agtcatggga caggatctcg cccgacagcg
      721 cgccatggcc cggcagtggc gccagcgcgc agtgacgcat gaagggcgcg acccggatga
      781 ttgcgttatg accccagtag tgcgactcgc cgagttgcca gtagtgcagg cccgccgtga
      841 acagcggccc gtagacctgg cttgcgaact gctgcgcacg cgcatgcagc gtggtgcgcc
      901 cgacggcctg cggggcggtc tggatgatgc cggcgtcggg ccgcgcctcc atcagacgtg
      961 cgagcgtcac caggcactcc ccggtcatca cgctgtcggc atcgaggacg atcatgtagc
     1021 ggtagttgcc gccccaccgc ctgcagaagt cgtcgatgtt gccgctcttg cgcttcacgc
     1081 gccgcgaccg ccgtcgatag aacaatcgat cctctgcacc cagcgtctct cgcaggccgc
     1141 cccacgcgtc gagctcggcc acacaggtgt ccgggtcgct gctgtcggag agcacgaaga
     1201 aatcgaagcg ctccagctcg cccgtgcgcg cgagtgactc ataggtcgct cgcaggccgg
     1261 caaagacgtg gtgcacgttt tcgttgcaga tcggcatgac gatcgccgtg cgcgcgtcgc
     1321 ccggcagcgg tccggcacgc gccaccgagc gcgaaatcag gaactcgtcg ccgccgcgca
     1381 tcagtatcca caggccggcc attgcggtcc agaagccggc tgcgacccag cacgacagca
     1441 gcgcaaacaa tgccagcaat gtgccttgca gccagccgtc cacggtggtc ggcagcaggt
     1501 cgctcatcag cgccgtggca tagagcgcgg gcaccagcga caacaccagc agcacaaagc
     1561 ggcgccgcgc tgccgacacc tcgagcgcgc tgaagccagg cacgttacgc ggggccgcgt
     1621 cggtcggtcg cagcgcgtgc cagggccgcg ccgccatcgg agtgcgcgcg acgggcggcg
     1681 tcgtgccagg cacggccgcc gtcaaatcgc cggaatgatg tagttccatg tttcactcaa
     1741 gggagaatcg gcgttgcgca gaacggcgcg caactcgatc ggtttgtcct tttcggttct
     1801 ccgaacctgc accaccatgc gccagccgcc gctgacctca tttctatgca cattcagtgc
     1861 cagtcgctgt cccccactga cggaaagatc acccaggacc gcagcgtccg cgggcaacct
     1921 gcgcagcgca ggcccatcga aatcgaccgt caacttgatg acatcctcgg gtagcgccgc
     1981 gtagccctga ccgcgccggg tctgaacgac ccatgcgttt ggcggacgcc gctcagtgcc
     2041 cttctgccac atcaggcgat agcggaactc gaagggctgg cccggacgca gcgtctgctg
     2101 cggcacccaa taggcgacga tgttgtcatt ggtctcgtcg ggcgtcggaa tctgcaccaa
     2161 ttcgacgcgc ccggcgcccc aggaacccac aggctcgacc cacaccgacg gccgcaactc
     2221 gtaacgagcc tcgaggtcct cgaagttgtc gaaggcgcga tcacgctgca tcaggccgaa
     2281 tccgcgcgga tcggtgacgg caaaggatgt gaccagaagg cggcgctggt tcaccagcgg
     2341 gcgccagttc cactcgccat tgccggcgtg gatcgcgagg tcgtcggagt cgtgcacctc
     2401 aggccgaaag tcctcggcgg aggcgcgctg gttttcaccg tggaagaaca tcgacgtcag
     2461 cggtgccagg ccgatctttg cgcccgcctc gcgcaggtgc agacgcgcgg tcacctcggt
     2521 aaccgtctcc gcacctgggc gcacgatgaa tcggtaggcg cccgccacgc gccgtgagtc
     2581 gagcaatgca taaacggtga gttcgcgtgc gttgcgcgcc ggtcgcacaa tccagaactg
     2641 ctcgaagcgt ggaaattctt ccccgccgcg ctcggcggtg tccaccgcca gccctcgcgc
     2701 cgaggcgccg aagcgctgac cctgcccaag agcgcggaaa taactcgcgc ccagaaacac
     2761 cagcagttcg tccttgtagc gctgtgtatt gaggggagtg tgtacccgga aacccgcgaa
     2821 gccgagcttt cgcaactgcg cccgatcaag gccgttcttg ccgtaatcga agtcggccgg
     2881 gtcgaacgga atctctcgca ctgttccgcc caccacttcg tggatgcgca ccggattggt
     2941 gtggaactgg ccaccgaggt ggaagaactg caactcgaac ggcaactttt ccgcgcgcca
     3001 cagcgaccgg gcggggtcga agcggatatc ccgcatctgg tcgtaattga gatccttcag
     3061 cgccttgggc aacgggggcg cgctggcgtt gaacggtttc gcggcgagcg cccgcgccag
     3121 cttgtcgacg ctgtcgaggt cgaagccctg cgcttgcgcc gcgggcccga ccagcacaag
     3181 cgcggcgacc gtcgcggcgc gcagcagcag accgacggac gcgagcaggc cgctcgcaga
     3241 aagcgaatcg ttcagtgtca tgcggggaag ttcctccagg cctcgcgtgg caaacggcgg
     3301 gcccggtcag gcttgaatcg taggcaattg cgctagtgcg tggggtctag aagaggacgg
     3361 aaccggcccg gcgaggtccc ggcattcccg acgagggcta cttttcctta cctttgccgt
     3421 tccccttggc cttgcccttg cccttgccgt gctcctgctc gtagtgcggc acgtagacgt
     3481 tgctgtacca gtcatccttt acaaagtaga cggggcgggc gcaggcatcg tacttctggc
     3541 aatgcttctt ccagttcttg gcatgccccg gcggcacgtg caggtaaatc ggctgcactg
     3601 gctccaccac ctgcacgcgt tggatcacaa ctggctgcgg atagatcagg accggacgcg
     3661 gggcattgcc aatgtctatc tggccataga agcccggctg gccaacgctg atcgaaattc
     3721 cgacatcggc ggccagtgca gccgtgaccg cagtcgttag caagatagcg agtagtgagc
     3781 gtttcatcgt gtcctttcga gtggctgtca ccttaacgcc cgaaggagcg atcaagacta
     3841 caacgccgtg aaggattagc cacatcccgt taatgaagag tggccgaccc gtccagcgtt
     3901 ggttggcccg gattgagcac tcgattcggc cgacgtcgaa cacttggggc ggctcacttc
     3961 ccggatggac accactcttc gtcgctgaca gcaattacct gcgccctgtc tgtccccagt
     4021 caatacgccc gacttgccga agcgaaggat ggagttcatg gcgcgcgcac ggctcaatca
     4081 atgaaggaat gaaggaggac ttcatgtttg cccctcggat gacgatttcc cttgcgggct
     4141 gggcgggtcg cctggcggcg gcgctgctaa tggcaatgcc tgtcgcggca catgctgcgg
     4201 acatctcctg gaaggcgaac atggcctcgg gagcagcgac cgttcgtggc agcgttgccc
     4261 gagtt
//
LOCUS       DATIRT010000059         4741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1187279, whole genome shotgun sequence.
ACCESSION   DATIRT010000059 DATIRT010000000
VERSION     DATIRT010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187279"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VLN25_01445"
     CDS             complement(<1..272)
                     /locus_tag="VLN25_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSQ09269.1"
                     /translation="MLKKVGMPTLADGTLFRTEAYIDGTWQAGASGARFAIANPADGV
                     KIAEVANCDRVDAEQAISAADRAWPAWRRKTAKERAAVLRTWFDLIL"
     gene            373..1281
                     /locus_tag="VLN25_01450"
     CDS             373..1281
                     /locus_tag="VLN25_01450"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HSQ09270.1"
                     /translation="MKFDSLAPDLQSWFRDMIKRGTGRDALLKSLLGAGYQERYARDA
                     IDTALKALPKPPPPPPIQPMAAPVAEAIPAVAGTAPTLSQQLVQMPNTIATVDREVHM
                     LFALTAPRIVLFGNLLSDDECEQMIELSRGKLARSSVVNNATGAYDIHPHRTSYGTYF
                     DRGENDLIRRLEKRIADLVQFPVENGEPIQILHYEPGGEYKPHYDYFDPRQPGNEQVL
                     TQGGQRIATLVMYLNDVEAGGSTVFPEVGVDVLPRRGNAVYFAYCSETGALDARSLHG
                     GSPVGAGEKWIATKWFRERHYVGSGV"
     gene            complement(1316..1391)
                     /locus_tag="VLN25_01455"
     tRNA            complement(1316..1391)
                     /locus_tag="VLN25_01455"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1356..1358),aa:Lys,seq:ctt)
     gene            complement(1418..1493)
                     /locus_tag="VLN25_01460"
     tRNA            complement(1418..1493)
                     /locus_tag="VLN25_01460"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1458..1460),aa:Lys,seq:ttt)
     gene            complement(1681..3252)
                     /locus_tag="VLN25_01465"
     CDS             complement(1681..3252)
                     /locus_tag="VLN25_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HSQ09271.1"
                     /translation="MPDEGSPKRRLRVPWSQRVTPADATLAVAPPEERSRLPRNPLRA
                     QFEQRSLFGEILDWMLAPLLLLWPMSVTVTYIVAQTIANAPFDRSLADAVGALSNHVR
                     DNRGSVTLLLPMSAREILRADAADSIYYMVLGVRGEFVAGDNDLPLPPDDTTPLPREV
                     QFRSDHVRGQEIRIAYTWVEFNRSGQREYALVQVAETLEKRSQLANEIIRGVIIPQFV
                     VLPLAVVLVWFGLTRGLAPLSDLQARIRSRQPDDLSPIDPRGAPEEIAPLVSSFNELL
                     ARLSLNMEMQQRFLADAAHQMKTPLAGLRTQAELALREIDPQELRRTLRHIASSTERS
                     THLINQLLALARAEYQASDPSGFEVVDLVPLARDLVRESVADAIGRGVDLGFEGTDTP
                     VRIVAAPMLVRELLKNLVDNALRYTPRGGSVTVRIASTGSTAYLDVEDTGPGIPENER
                     QRVFDRFYRILGTNVDGSGLGLAIVREIAEQHGALIRVSSNPASLDERWPGTLISVEF
                     EALDTAQPTVDLSAE"
     gene            complement(3267..3968)
                     /locus_tag="VLN25_01470"
     CDS             complement(3267..3968)
                     /locus_tag="VLN25_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSQ09272.1"
                     /translation="MRILIAEDDAVLADGLSRSLRAGGYAVDVVSSGDDADTALAAQP
                     FDLLILDIGLPRLSGLEVLKRLRGRNSTVPVLILTAADSVEQRVKGLDLGADDYMAKP
                     FALSELEARVRALTRRAAGGAQTVLKHGPLSLDQVGHVAFLHDQMLDLSARELSLLEI
                     LLQRPGRLVSKEQLVDHLCSWGEEVSTNAIEVYVHRLRKKIETDAVRIVTVRGLGYYL
                     ERFADPAAEVAAGSA"
     gene            4179..>4741
                     /locus_tag="VLN25_01475"
     CDS             4179..>4741
                     /locus_tag="VLN25_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HSQ09273.1"
                     /translation="MDDGRSMVVTDGKERQKALAAALSQIEKQFGKGSVMRLADGEVV
                     EDIQVVSTGSLGLDIALGVGGLPRGRCVEVYGPESSGKTTLTLQVIAEMQKIGGTCAF
                     IDAEHALDIQYAQKLGVNLQDLLVSQPDTGEQALEITDALVRSGSVDLIVIDSVAALT
                     PKAEIEGEMGDSLPGLQARLMSQALRKL"
BASE COUNT          856 a         1513 c         1558 g          814 t
ORIGIN      
        1 aggatcaggt cgaaccaggt gcgcaatacg gccgcgcgct ccttggcggt cttgcgtcgc
       61 catgcgggcc aggcccgatc ggcggcggag atcgcctgct cggcgtccac gcgatcgcag
      121 ttggcaactt cggcaatttt cacgccgtcc gccggattgg cgatagcaaa gcgcgcaccc
      181 gacgcccccg cctgccatgt gccatcgatg tacgcctcgg tcctgaaaag tgtcccgtcc
      241 gccagcgttg gcattcccac tttcttcagc atgtctcgcc tccgactatc cctggcagca
      301 agaatactgc cgaagcggta cgggcaccgt gcccgctaaa cttcgtgctc tcccctagct
      361 ccttgcatca acatgaagtt cgacagcctt gctcccgacc tgcaatcctg gttccgcgac
      421 atgatcaagc gcgggaccgg ccgggacgcg ctgctgaagt cgctgcttgg cgcgggctat
      481 caggaacgtt atgcgcgcga tgccatcgac acagcgctga aggcgttgcc gaagcctcct
      541 ccgccgccgc cgatccagcc gatggccgcg cctgttgcag aagcgatccc ggccgtcgca
      601 ggcacggcgc cgacgctcag ccagcaactg gtgcagatgc cgaacaccat cgccaccgtc
      661 gatcgcgaag tccacatgct gttcgcgctg actgcgccgc gcatcgtgct gttcggcaac
      721 ctgctctcag acgatgaatg cgagcagatg atcgaactgt cgcgcggcaa gttggcgcgg
      781 tccagtgttg tcaacaacgc gaccggggcc tacgacatcc atccccaccg caccagctac
      841 ggcacctatt tcgatcgcgg cgaaaacgac ctgatccggc gcctcgagaa gcgaatcgcc
      901 gacctcgtgc agttcccggt ggaaaacggc gagccgatcc agatcctgca ctatgagccc
      961 ggcggagagt acaagccgca ctacgactac ttcgatccca ggcagcccgg caacgagcag
     1021 gtcctgacgc aggggggaca gcgcatcgct actctggtga tgtacctgaa cgatgtcgag
     1081 gccggcggtt cgacagtctt ccccgaggtc ggcgtcgacg tgctgccgcg ccgtggcaac
     1141 gcggtctact ttgcctactg ttcggaaaca ggcgcgctcg atgcacgctc actgcacggc
     1201 ggcagccccg tgggtgccgg ggagaagtgg atcgcgacga agtggttccg cgaacgccac
     1261 tacgtcggca gcggcgtctg acctcgggct cggcgggcac agacaaggaa aatcgtggtg
     1321 ggtcgtgaag gattcgaacc ttcgacctac ggattaagag tccgctgctc taccaactga
     1381 gctaacgacc cgatcttcct gcgtctttct accttgatgg taggtcgtgc gggattcgaa
     1441 cctgcgacca acggattaaa agtccgctgc tctaccagct gagctaacga cccgagccct
     1501 ttgacatgct ctgcgatgcc cgcgatctcg ggacgtgata tcggggggcg cgatggacgt
     1561 tgtcttgacg gccgaccgca tcgagagcac gaaattatgc cgatcgacag cgcacgggtc
     1621 aaaccggtgc atgccggcga aacggggcgc actcccgcct gtcgcgtgaa caggccccaa
     1681 tcactccgcc gacaggtcga cagtcggctg ggccgtgtcc agggcctcga actcgacgct
     1741 gatcagcgtg ccgggccagc gttcgtcgag gctcgccggg ttgctgctga cacggataag
     1801 cgcgccgtgc tgttctgcga tctcgcgcac gatcgccagt cccaggccgc tgccgtcgac
     1861 attagtgccg aggatgcggt agaagcggtc gaagacgcgc tgacgctcgt tttccggaat
     1921 tcccgggccg gtatcttcga cgtccaggta cgccgtgctg ccggtgcttg cgatgcgcac
     1981 ggtcacgctg cccccgcgcg gtgtgtagcg caatgcgttg tccacgagat ttttcagcag
     2041 ttcgcgcacc agcatcggcg cggcgacgat gcgcaccgga gtgtctgtcc cttcgaaccc
     2101 gagatcgacg ccgcgtccga tcgcatcggc cactgactcg cgcacgagat cgcgcgccag
     2161 cgggacgagg tcgactactt caaagccgct cgggtcgctc gcctggtatt cggctcgcgc
     2221 cagcgccagt aactggttga tcaggtgcgt cgaacgttcg gtcgacgacg caatgtggcg
     2281 cagagtgcgc cggagttcct gcggatcgat ctcgcgcagc gcaagctccg cctgcgtgcg
     2341 caggcctgcg agcggagtct tcatctggtg cgcggcgtcg gcgaggaagc gctgctgcat
     2401 ttccatgttg agcgacaggc gcgccaacaa ctcgttgaag gaactgacca gcggcgcaat
     2461 ctcctcgggc gcgccacgcg ggtcgatcgg cgacaggtcg tccggctggc gcgaccggat
     2521 ccgtgcctgc aggtccgaca ggggcgcgag cccgcgtgtg agtccaaacc acaccagcac
     2581 gaccgcgagt ggcagcacga cgaactgcgg aatgatgacg ccgcggatga tttcattggc
     2641 cagctgcgag cgtttttcca gcgtctcggc gacctgcacg agcgcgtact cgcgctggcc
     2701 gctgcggttg aactccaccc aggtgtaggc aatgcggatc tcttgtccgc gcacatgatc
     2761 cgatcgaaac tgcacttcgc ggggcagggg ggtggtgtca tccgggggca aaggcaggtc
     2821 gttgtcgccg gcgacgaact cgccccttac gcccaggacc atgtagtaga tgctgtcggc
     2881 cgcatcggcc cgcaggatct cgcgggcgct catcggcagc agcagcgtca cgcttccacg
     2941 gttatctcgg acatggttcg acagcgcgcc cactgcatcc gcaagactgc gatcgaacgg
     3001 tgcgttggcg atggtctggg caacgatgta ggtgaccgtc acgctcatgg gccacagcag
     3061 caacagtggc gcgagcatcc agtccaggat ttcgccgaag agcgatcgtt gctcgaactg
     3121 tgcgcgcagc ggattgcgcg gcaggcgcga gcgttcctcc ggcggagcca ctgccagcgt
     3181 cgcatcggcg ggcgtgacgc gttgggacca agggacccgc aggcggcgct tgggactgcc
     3241 ctcgtcaggc atcggtgtgc cggtgctcaa gcgctgccgg cggcgacttc agcggccggg
     3301 tcggcaaagc gctcgaggta atagccgagt ccgcgcacgg tcacgatgcg gaccgcgtca
     3361 gtctcgatct tcttgcgcag acgatggacg tagacctcga tcgcattggt gctgacttcc
     3421 tcgccccagc tgcataggtg atcgaccagt tgttccttgc tgacgagccg gccgggacgc
     3481 tgcagcagga tctcgagcag gctcagttcg cgcgccgaca agtcgagcat ctggtcatgc
     3541 aggaacgcga catgcccgac ctgatcgagg gacaacggtc cgtgcttgag cacggtctgg
     3601 gcgccgccgg cggcacgccg cgtcagcgcg cgcacccggg cttcgagttc ggacaacgca
     3661 aacggcttgg ccatgtagtc gtcggcgccg aggtcgaggc ccttgacgcg ctgctcgacg
     3721 ctgtcggctg cggtcagtat cagcaccggc acggtcgaat tgcggccgcg caggcgcttg
     3781 agcacttcga gtccggaaag ccgcggcagc ccgatgtcca ggatcagcaa gtcaaagggc
     3841 tgggcagcca acgcagtgtc ggcgtcgtcg ccgctcgaaa ccacgtcgac ggcatagccg
     3901 cctgcccgca gcgagcggga caagccgtca gccaggacgg catcgtcttc ggcgatcagg
     3961 atgcgcatga gtcgagaggc tatgcttggg tccaggaagc ccgagcaagc gccgcagtct
     4021 agggcgggcc aaagtcccgc tgcaaggagc gtcgcaccgc cgggcctcgc ggatgagtcc
     4081 ccggttgaca agcgatactg aaagtatcta cagtgcatgt actgtaatta acttcagtgc
     4141 tccgggtgtc ccggggccaa gcgcgagcgg aggcggcaat ggacgacgga aggagcatgg
     4201 tggtcacgga cggcaaggag cggcagaagg cgctggccgc ggcactgtca cagatcgaga
     4261 agcagttcgg caagggttcc gtgatgcgcc tggccgatgg ggaggtcgtt gaagacatcc
     4321 aggtggtttc caccggttcg ctgggactcg acatcgcact gggggtcggt ggcttgccgc
     4381 gcggtcggtg cgtcgaggtc tatggtccgg agtcgtcagg aaagacaacg ctgacgctgc
     4441 aggtcatcgc cgagatgcag aagatcggcg gcacctgcgc cttcatcgat gccgagcacg
     4501 cgctcgacat ccagtacgca cagaagctcg gcgtcaatct gcaggacctg ctggtgtcgc
     4561 aaccggacac cggtgagcag gcgctagaaa tcaccgacgc cctggtgcgt tccggttcgg
     4621 tcgacctgat cgtgatcgac tcggtcgccg cactgacgcc caaggcagag atcgaaggcg
     4681 agatgggtga ctcgctgccg ggactgcagg cgcgcctgat gtcgcaggcc ctgcgcaagc
     4741 t
//
LOCUS       DATIRT010000060         3426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_302384, whole genome shotgun sequence.
ACCESSION   DATIRT010000060 DATIRT010000000
VERSION     DATIRT010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302384"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..921
                     /locus_tag="VLN25_01480"
     CDS             196..921
                     /locus_tag="VLN25_01480"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HSQ09274.1"
                     /translation="MPSSPRLPLIDVLRGFAVAQMIVYHFIYDLNYYGWLKLSMLTDQ
                     PWVGWRTSIVTQFVLLVGVGLVLRTSFKPGLRDFWRRWTQIAAAALLVSVGSAAVFGP
                     RFIWFGILHFVAAALILARPFVRIGPWNVAIGLAVIAFGLLYANPIFNDTPANIIGLV
                     TFKPKTEDYVPLLPWLGVVLLGAGLGAMWQQRAWSIPAALRSLNNRPPRLLMLLGTWA
                     LTVYLVHQPLLMGALWLARRIGL"
     gene            complement(1002..2018)
                     /locus_tag="VLN25_01485"
     CDS             complement(1002..2018)
                     /locus_tag="VLN25_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HSQ09275.1"
                     /translation="MSPPRHSHSASSDANSDDPAVRGERHRHSHLGDLRDGDSAYGHH
                     SHSGGTRPLAIAVALTFGYALVEVAGGLWSGSLALLADAGHMATDSAALLFALAANVI
                     ARRPVSDRHSFGLARVEVIAAFVNALAMLAVVAWIFFEAFDRISAPVPVKGLEVFGIA
                     AIGLAVNLVVAWTLSRDRENVNTRAAFLHVLGDLLGSVAAIAAGLIIHFGGPTLVDPL
                     LSMLVGGLILRSTTAVLRETTLVLLDSVPQNVDYPTVGKALAKVPGIASVHDLHVWSM
                     VPGRSALSAHVMVGDNEQWPVVLHRARTMLKREFQIDHVTLQPEWLRKDAPAQMIPLE
                     RRPH"
     gene            complement(2047..2697)
                     /locus_tag="VLN25_01490"
     CDS             complement(2047..2697)
                     /locus_tag="VLN25_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HSQ09276.1"
                     /translation="MAWTREEMAARAAQELRDGFYVNLGIGLPTLVANYIPAGMDVML
                     QSENGLMGIGPFPLESEVDADLINAGKQLVTALPGSSFFSSADSFAMIRGGHINLAIL
                     GAMQVAENGDLANWMIPGKLVKGMGGAMDLVSGVERVIVLMEHCAKNGEHKILQRCTL
                     PLTGRGVVNRIVSDLGVFDIGPDGLSAVELAPAVTGEEVRLKTGCHVHMTRLPATA"
     gene            complement(2706..3416)
                     /locus_tag="VLN25_01495"
     CDS             complement(2706..3416)
                     /locus_tag="VLN25_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HSQ09277.1"
                     /translation="MKKVFPTAAAALADVVKNNQTIAVGGFGLCGIPEALIAALRDSG
                     VKGLTCVSNNAGVDGFGLGLLLTTRQIHKMISSYVGENKEFERQYLSGELELEFTPQG
                     TLAERMRAGGAGIPAFFTRTGVGTIVADGKPSQVFDGVWGSKEYVMERSIRADVSLVK
                     AWKADHEGNLVYRKTSRNFNPVVAMCGKVTVAEVEEIVENGDLEPDNVHTPGIFVQRI
                     VLNRTPEKRIENRTVRAK"
BASE COUNT          591 a         1145 c         1056 g          634 t
ORIGIN      
        1 aagagcgcac agctgttgtc ggtgtggtcg cgcttagatt gtcgtgtgtc gacttccgga
       61 gcgttctgat tgttcctctt tcctctccac taacctgcac gatgggcatc tgaaataggg
      121 cccatgccat tcccagctca acatcaacgg aagctgaaca cctcgcggta gcatgccggg
      181 cgccttcagg taacgatgcc ctcctctccc cgcctgccac tgatcgatgt cctgcgcggc
      241 ttcgccgtgg cgcagatgat cgtgtatcac ttcatctacg acctgaatta ctacggctgg
      301 ctaaagctca gcatgctgac cgaccagccg tgggtggggt ggcgcacttc catcgtgacg
      361 cagttcgtgc tgctggtggg tgtcggcctg gtgttgcgga cctcattcaa gcctggcttg
      421 cgtgacttct ggcggcggtg gacgcagatc gccgccgcgg cactgctcgt gtcggtcggc
      481 agtgcggcgg tgttcggccc gcgcttcatc tggttcggca tcctgcactt cgtcgccgcg
      541 gcgctgatcc tggcgcggcc attcgttcgc attgggccct ggaacgtggc catcggcctt
      601 gcggtgatcg cattcggatt gctctatgcg aaccccatct tcaacgacac gccggcgaac
      661 atcatcggcc tggttacatt taaaccaaag accgaggatt acgtaccgct gcttccgtgg
      721 ctgggggtcg tgctgctcgg cgcaggcctt ggggcgatgt ggcagcaacg cgcctggagc
      781 attcccgcag cacttcgatc actgaacaac aggccgcccc gcctactgat gctgctcgga
      841 acctgggcac tgacggtcta cctcgtgcac cagcccctcc tgatgggggc gctgtggctg
      901 gcgcggcgca tcggcctctg aacgaccgct ggctgtggca gcacccaggg atcaaccgtt
      961 gacggaaacg caaacgtcgc tggtcctaca gaaccgtccg ctcaatgcgg ccgccgttcg
     1021 agcggaatca tctgcgccgg cgcatccttg cgcagccact cgggctgcag cgtgacgtgg
     1081 tcgatctgga actcgcgctt gagcatggtg cgtgcccgat gcagcaccac gggccactgc
     1141 tcgttgtcac ccaccatcac gtgcgcggaa agcgctgatc gcccgggcac catcgaccag
     1201 acatgcaggt cgtgcacgga cgcgatcccg ggtactttcg ccagcgcctt tcccacggtc
     1261 gggtaatcga cgttctgcgg gacactgtcg agcagcacca gcgtggtctc ccgcagtacc
     1321 gccgtggtgg atcgaaggat cagtccaccg acaagcatcg acagcagcgg atcgaccagc
     1381 gtcggaccgc cgaagtgaat gatcagtccg gcggcgatcg ccgcaaccga ccccagcagg
     1441 tcgcccagca catggaggaa tgccgcacgc gtattgacgt tctcccggtc gcgcgacagc
     1501 gtccaggcga cgaccagatt gaccgccagc ccgatcgccg cgatgccgaa cacctcgagc
     1561 cccttcacgg gtaccggtgc gctgatgcgg tcgaaggctt cgaagaagat ccacgcgacc
     1621 accgccagca tcgccagcgc attgacgaag gcggcaatga cctcgacgcg agccagcccg
     1681 aaagaatgcc gatccgagac gggccgtcgc gcgatgacat tggcggcgag cgcgaaaagc
     1741 agcgcagcgg agtcggtggc catgtgcccc gcatcggcca gcagcgcaag cgaacccgac
     1801 cacaagccac ccgccacttc gaccagcgca tagccgaacg tcagcgcgac cgcgatcgcg
     1861 aggggccgcg tgcctccgga atgcgaatgg tgcccatagg cactgtcgcc gtcgcggaga
     1921 tcgccgaggt gcgagtgacg gtggcgttcg ccgcggaccg cagggtcgtc ggagttcgca
     1981 tccgacgatg cgctgtgaga gtggcgcggc gggctcatgg ccctcgcccc ccgagaagga
     2041 tcgggatcag gccgtggctg ggagacgcgt catgtggaca tggcacccag tcttcaggcg
     2101 cacttcctcg ccggtgaccg ccggagccag ctcgactgct gacagtccat ccggtccgat
     2161 gtcgaatacg ccaaggtctg agacgatgcg gttgaccacg ccacgacccg tgagcggcag
     2221 cgtgcaacgc tgcagaatct tgtgttcacc gttcttcgcg caatgctcca tcagcacgat
     2281 gacacgctct acgccggaaa cgagatccat agcgcccccc atgcccttga ccagtttgcc
     2341 ggggatcatc cagttggcga ggtcgccatt ttccgccacc tgcatcgcgc cgaggatcgc
     2401 gaggttgatg tggccaccgc gaatcatcgc gaacgaatcc gcgctcgaga agaaactcga
     2461 tcccggcagt gcggtgacca gctgcttgcc ggcgttgatc aggtcggcgt cgacttcgct
     2521 ttcgagagga aacgggccga tgcccatcag gccgttctcc gactgcagca tgacgtccat
     2581 gcccgccggg atgtagttgg ccaccagcgt cggcaggccg atgccaaggt tgacgtagaa
     2641 gccgtcgcgc agttcctgcg cggcgcgcgc cgccatttct tcacgggtcc aggccaggtt
     2701 gtctcctact tcgcgcgcac ggtgcggttt tcgatgcgtt tctcgggtgt tcggttcagc
     2761 acgatccgct gcacgaagat cccgggcgta tggacattgt cgggctcgag gtccccgttc
     2821 tcgacgatct cctcgacctc ggcgaccgtg actttgccgc acatcgctac gaccggattg
     2881 aagttgcgcg atgtcttgcg gtagacgaga ttgccctcat ggtccgcctt ccacgccttg
     2941 accagcgaga cgtcggcgcg gatcgagcgc tccatgacgt attccttgct gccccacaca
     3001 ccgtcgaaca cctggctcgg cttgccgtcg gcgacgatag tgccgacacc ggtgcgcgtg
     3061 aagaacgccg ggatgcccgc tccgccggcg cgcatccgct cggccagagt gccctgcggt
     3121 gtgaactcca gctcgagttc ccctgacagg tactggcgct cgaactcctt gttctcaccg
     3181 acgtacgacg agatcatctt gtggatctgg cgcgttgtga gcagcagccc gaggccgaag
     3241 ccgtcgacgc ccgcgttgtt gctgacacag gtcagcccct tgactccgct gtcgcgcagc
     3301 gcggcgatca gcgcttccgg aatgccgcac agtccgaagc cgcccacggc aatcgtctgg
     3361 ttgttcttca ccacatcggc cagtgccgcc gcggccgtcg gaaacacctt cttcatcccg
     3421 ccctcc
//
LOCUS       DATIRT010000061         8293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1482407, whole genome shotgun sequence.
ACCESSION   DATIRT010000061 DATIRT010000000
VERSION     DATIRT010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8293
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482407"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..1382)
                     /locus_tag="VLN25_01500"
     CDS             complement(189..1382)
                     /locus_tag="VLN25_01500"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HSQ09278.1"
                     /translation="MRRSRTEVQPDVSPRDSRTIGRRARRPGPDADRPVLASSSLGDL
                     GIGPVSTVPAALRACGVEPASVLREVGLASHIFDDSANRMTFHALGRLLEACVAATGR
                     PHFGLLTSTHFDLTKLGPLGYLMSNEATVRAALRTLVLYLHVHDRGAIVTLDRIDGRL
                     TALTYAVCVPETPALGLIYDATMMIGHRVMKTLCGPGWKAKEVRLARAAPADPRLYRQ
                     MFEAPVRFNVALSALVFESHWLDRPVPRADPVLHRVLTQMLTALAGDIPNRLSDKVRR
                     ALGNSVLAGTADAAHIADLFSLSERSLRRRLAREGVTLQELISQARLRVTQQLLEQTG
                     MSLRDISGALNYSDPSALSRAFRGWTGFAPSEWRRTRATQGLAAATTAKSAEVKASGR
                     PVDRG"
     gene            1584..4085
                     /locus_tag="VLN25_01505"
     CDS             1584..4085
                     /locus_tag="VLN25_01505"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HSQ09279.1"
                     /translation="MHLKFGAPLALIATVAMMGAAGGQQITGTLGSPSATTTVPNTQL
                     PPPDPKFGGVIKDGALQSKPWWPPRVVPPKGAPNVLLIITDDAGFGVPSTFGGVVPTP
                     SMDRIAKEGLRYNRVFSTALCSPTRAALITGRNHHSAGFGVISEQSTGFPGYNSIIGK
                     DKATIGRILLDNGYSTSWFGKNHNTPAFAASQVGPFDQWPSGMGFEYFYGFVGGDANQ
                     WEPNLFRNTTQIYPFQGKPGWNLVTGMADDAIDYMTRIHQTDPSKPIFIKYAPGATHA
                     PHHPTKEWVDKIAAMKLFDGGYEKLRETIFENQKRLGVIPKDTKLGPWPSQMLKPWDQ
                     LTADEKKLFIRQVEVFAAYAAYSDYEIGRVIQHFQDLGKLDNTLIIYINGDNGTSAEG
                     GPNGTPNEVAFFNGVQVPVDVQMKFYDVWGTERTYNHMSAGWSWAFDTPFSWFKQNAS
                     ALGGVNQNMVVSWPARIKDKGALREQFVHVIDVVPTILEAAGIKAPLAVDGIKQAPIE
                     GTSFAYTFDEKNAKAPQRHKTQYFEMMGQWALYDDGWFLSTKVNRAPWDAFGPANPDP
                     LNNQVLELYNLNTDFSQTTDVAAKNPAKVKELKQKFIAEARKHQVLPFDASVAARIVA
                     PRPNITAGRTEFVYTRPMVGLPQGDSPFILNSSYTITVDIEVPAGGAEGMMVTSGGRF
                     AGYGFYLLKGKPVFLWNLVDLERLKWEAPEALPPGRHTVEFDFKYEGLGAGTLAFNNF
                     SGVGRPGTGTLRVDGKEVATKRMDKTLPMILQWDESFDIGSDTLTGVNDADYKPPFPL
                     TAKLNKLTIKMNRPELSPADIKKLQSAMTEKAKRD"
     gene            4260..4703
                     /locus_tag="VLN25_01510"
     CDS             4260..4703
                     /locus_tag="VLN25_01510"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HSQ09280.1"
                     /translation="MKRFVGNLFAVLLALALTACASSGGTSYPPEIRQGRIEQITPIQ
                     LDHPHQIGVGAILGGAAGAGIGSLIGAGTGRDVAIAVGAIAGAIGGQYAQNRYETRKP
                     GQQIVVRLKSGVLVVVTQAEDPALRLGQNVYVQGAGADARVVPQY"
     gene            4851..7535
                     /locus_tag="VLN25_01515"
     CDS             4851..7535
                     /locus_tag="VLN25_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09281.1"
                     /translation="MKVCFRMLMLTWVIGAGLLGLAACANQGADASTLPPPMDASYAT
                     PAATPTTAGTMVPVVYEPNASMTASTANYSSLAGQAAVAAPTSPPTAPRSADPWPRDV
                     TVSDADALIYQPQIESWTGNQMAWRVAVALRPAGAKAETFGVLWGSARTEVDRSTRTV
                     SLEDVSVSQIKFPTLPDNGAAYLAGLRQALHGALATIALDSVEASLAASQSVKPNGHA
                     VRNEPPKVIVAYGPALLIPIEGQPVVRAMSDARFERVINTQALIARRRFGDTWFLKLY
                     DGWVSSTSLAGPWTQAGFVPWGLEDASKSLIKAGTVDALNGTGATPKPTLANGVPAIH
                     VTEVPTELIVFKGQPNLVPITGTPLLWATNSATDVLVDTTNNAYYVLLSGRWYRAAGL
                     AGPWSFIASNALPASFKQIPAKGSPASIVLASVAGTPQAQEAVIANSIPQTASVARAG
                     GPIFAPEFDGAPVWQPVTSTSLFYAANARIPVIQVNPSSYYAVQAGVWFSAPVVTGPW
                     VVATTVPEVIYTIPVASPLHYVTYVRVYGYKDDLVYVGYTPGYMGTVVDVGGTVVYGT
                     GYSYSPWIGTVWYGAPVTWGVAAAPVYNPYVGFTYGFGIGLATAAWAEPYWGGAYYHP
                     GYYGYPCCGSASANVYRNWGTGVSSGTRTWYNNAGGSFGTAASGSYATDRGTTGTYNA
                     QRSWNPYTGQGSQGYNRTVNTASGTTGSVNRNETYNAQTGQRGYGSNVEATGAGGSSI
                     DRNVSATAGPQGVDRSASTTTYNANTGQTKTWSDGTPQNTHVAGSDGNVYRSDGSGGW
                     QQHSTSGWGSASGNSAWASQEQQARSTAADRTNSYGGGGWDRSGSGSDSSRYGGGDSW
                     GNRFGGSAGGSGSWDNRFGGGSYSGRFGGGGFRGGGRR"
     gene            7568..7873
                     /locus_tag="VLN25_01520"
     CDS             7568..7873
                     /locus_tag="VLN25_01520"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSQ09282.1"
                     /translation="MSDAVLTKDESAGHYRLMRDGAEVGYVEYDRVGEYSILIKHTEV
                     AAEHEGQGLGALLVSGVLDDVRRQGKTVIPICPYALNYIRKHRDYIDVVHADMRSTI"
     gene            8020..>8293
                     /locus_tag="VLN25_01525"
     CDS             8020..>8293
                     /locus_tag="VLN25_01525"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSQ09283.1"
                     /translation="MSDLRDVDIAVVGAGVAGMACALGLSQQGLRVALIGPRVVPHAP
                     STAAPYDARIYAVAPATIELLQRLSVWNRVDATRASPVERMRVFGDR"
BASE COUNT         1581 a         2641 c         2713 g         1358 t
ORIGIN      
        1 gtcgcgcgcc cgtggccgcg caacgatcca ctacctgacg cctcacggac gcggcaagcc
       61 ggcaacgctt tcgacgttgg tgcgacagag ctgagtcgca cccccttgcg agggcaggca
      121 gccagcgacc gaccgtcggc atgatcggcg atgaagcgtt agcgaaggcc agcccccgaa
      181 tgagcaacct atcctcgatc aaccggccgg cccgatgcct tgacttcggc tgacttcgcc
      241 gtggtcgcgg cggcgagccc ttgcgttgcg cgcgtgcggc gccattcgct cggcgcgaac
      301 cccgtccatc ctcgaaacgc ccgcgacaac gcgctcggat cggagtaatt cagcgcacca
      361 gaaatgtccc gtagcgacat gccggtctgt tcgagcagtt gctgcgttac ccgcaggcgc
      421 gcttggctga tcaactcctg cagcgttaca ccctcgcgcg ccaggcgacg ccgaaggctg
      481 cgttcgctca gcgaaaacaa atctgcaata tgcgcagcgt cagccgtacc ggcgagcacg
      541 ctgttgccca gcgcgcgacg gaccttgtca ctcaggcgat tcgggatgtc tccagcaagg
      601 gcagtgagca tctgagtcag cacccgatgc agtaccggat cggcgcgggg tacgggacga
      661 tcgagccaat gcgactcgaa gaccagtgcc gacaaggcca cgttgaaccg taccggggct
      721 tcgaacatct ggcggtaaag gcgcggatcg gcgggtgcgg cacgcgcaag gcggacctct
      781 ttcgccttcc atccggggcc gcacagcgtt ttcatgacac ggtgaccgat catcatggtg
      841 gcgtcgtaga tgagcccgag cgccggcgtt tcaggtacgc acaccgcgta ggtaagagca
      901 gtgaggcgac catcgatgcg gtccagcgtg acgatagcgc cccggtcatg cacatgcaga
      961 tacagcacca gcgtcctcag ggcagcgcgc actgtcgctt cattgctcat caggtagccc
     1021 agagggccga gcttggtcag atcaaagtgg gtgctggtca gtagcccgaa gtgcggccgg
     1081 cccgtggcgg ccacgcaagc ctcgagcagg cggccaaggg cgtgaaacgt catgcgattg
     1141 gcgctgtcgt cgaaaatgtg actggcgagc ccgacttccc tcaacacgct ggcgggctcg
     1201 acgccgcagg cgcggagcgc cgccgggaca gtcgagaccg gcccgatgcc gaggtcgccc
     1261 agtgaactcg atgctaaaac cggacgatca gcatccggtc ctggccggcg cgcgcgcctg
     1321 cctattgttc gagaatcgcg cggtgacacg tcgggttgaa cctcagtgcg tgaacgacgc
     1381 atcgttcaaa ttttggccgg aattgtcaag ctcttgtcat ttgagtacag ctaaattgcc
     1441 gagcggctgg gactaaccct gatatgcgcc tgtcaaccac tgatgtgact ggcgggcgcg
     1501 tcgcgggtga gtgagccgca ggtcatcaat ccaaagcgtc gaaacggagc aaggctgccg
     1561 agcccggggc cagaggaagg actatgcatc tgaaattcgg ggcgccgctg gcgctgatcg
     1621 caacggtggc gatgatgggc gcggccggcg gccagcagat caccggcacg ctgggctcgc
     1681 ccagcgccac aacgacggtc cccaacaccc agttgccgcc gccagatccg aaattcggtg
     1741 gtgtcatcaa ggacggcgcg ctgcagtcca agccctggtg gccgccgcgc gtggtgccgc
     1801 cgaagggcgc gcccaacgtg ctgctgatca tcaccgacga cgccggcttt ggggtgccca
     1861 gcaccttcgg cggcgtagtt ccgacgcctt cgatggaccg catcgcgaag gaagggctgc
     1921 gctacaaccg cgtgttctca accgcgctgt gctcgccgac gcgcgctgcg ctgatcaccg
     1981 gacgcaatca ccactcggcg ggatttggcg tgatctccga gcagtcgacc ggattcccgg
     2041 gctacaacag catcatcggc aaggacaagg caacgattgg ccgcatcctc ctcgacaatg
     2101 gctattcgac ctcctggttc ggcaagaacc acaacacgcc ggcgttcgct gccagccagg
     2161 tcgggccctt tgatcagtgg ccgtctggaa tgggattcga gtacttctat ggcttcgtgg
     2221 gcggcgacgc caaccagtgg gaaccaaacc tgttccgcaa cacgacccag atctatccgt
     2281 tccagggcaa acccggctgg aacctcgtca ccggaatggc cgacgacgcg atcgactaca
     2341 tgacgcgcat ccaccagacc gacccttcca agccgatctt catcaagtac gcgcccggcg
     2401 ccacgcacgc gccgcatcat ccgaccaagg agtgggtcga caagatcgcc gcgatgaagc
     2461 tcttcgacgg aggttacgaa aaactgcgcg agacaatctt cgagaatcag aaacgcctgg
     2521 gcgtgatccc taaggacacg aagctcgggc catggccgag ccagatgctc aagccctggg
     2581 atcaactgac ggctgacgag aagaagcttt tcatccgcca ggtagaggtg ttcgccgcct
     2641 atgccgccta cagcgactat gagatcggcc gcgtaatcca gcacttccag gacctgggca
     2701 agctggacaa cacgctgatc atctacatca acggcgacaa cggcaccagc gccgaaggcg
     2761 gcccgaacgg cacgccgaac gaggtggcct tcttcaacgg cgtacaggtc cccgtggacg
     2821 tgcaaatgaa gttctacgat gtctggggca ccgaacggac ctacaaccac atgtcggccg
     2881 gctggtcgtg ggccttcgac acgccgtttt cgtggttcaa gcaaaacgcc tcagcgctgg
     2941 gcggcgtgaa ccagaacatg gtggtgtcct ggccggcgcg catcaaggac aagggcgcgc
     3001 tgcgcgagca gttcgtgcac gtgatagacg tcgtgccgac gattcttgag gccgcgggca
     3061 tcaaggcacc gctcgcggtg gatggcatca agcaggcccc gatcgagggc acgagcttcg
     3121 cctacacgtt cgatgaaaag aacgccaagg cgccgcaacg acacaagacc cagtacttcg
     3181 agatgatggg ccagtgggcg ctctacgacg acggctggtt tctcagcacc aaggtcaacc
     3241 gcgcgccgtg ggacgccttc ggaccggcca accccgaccc gctgaataac caggtgctgg
     3301 agctgtacaa cctgaacacc gatttcagcc agaccaccga cgtggcggcc aagaacccag
     3361 ccaaggtaaa ggaactgaag cagaagttca tcgccgaggc caggaagcat caggtgctcc
     3421 cgttcgacgc atcggtcgcg gcgcgcatcg ttgccccgcg gccgaacatc actgcagggc
     3481 gcaccgagtt cgtttacacc cggccgatgg ttggtctgcc gcagggcgac tcgccgttca
     3541 tcctgaactc gtcctacacc atcaccgtcg acatcgaggt gccggccggc ggcgccgagg
     3601 gcatgatggt gacctcgggc gggcgcttcg ccggctacgg tttctacctg ctcaagggca
     3661 agccggtatt cctgtggaac ctggtcgacc ttgagcgatt gaagtgggag gcgcccgaag
     3721 cactgccgcc cggccgacac acggtcgagt tcgacttcaa gtacgagggg ctcggcgccg
     3781 gcacgctcgc gttcaacaac ttcagcggtg tcggccgacc cgggaccggc acgctcaggg
     3841 tcgatggcaa ggaggtcgcg acgaagcgga tggacaagac gctgccgatg atcctgcagt
     3901 gggacgagag cttcgacatc ggctccgata ccctgaccgg cgtgaacgac gccgactaca
     3961 agccgccatt cccgctgacc gcgaagctga acaagctgac gatcaagatg aaccggccgg
     4021 agctgtcacc agctgacatc aagaaactgc agtcggcaat gacggagaag gcgaagcgcg
     4081 actgatccct tggacctgaa tgcacctgcc tgcccagcca cgtcggcagg cgggctgcag
     4141 caaggcaact cccggtgcgg gccgaagcag gcgtcgctga acgtgcgtat cggcaacttg
     4201 acgccgtccc cagcaaggca gtcactgcaa cgattcactc aacctgatga gagaacgaca
     4261 tgaaacgttt cgtaggcaat ctctttgccg tgctcctcgc gcttgccttg accgcctgtg
     4321 ccagctctgg cggaacttcg tatccaccgg aaatccggca aggacggatt gaacagatca
     4381 cgccgatcca gctcgatcat ccccatcaga tcggagtcgg tgcgattctc ggtggggcgg
     4441 cgggcgccgg gatcggcagc ctgatcggtg ccggcaccgg gcgtgatgtc gcaatcgcgg
     4501 tcggtgccat cgccggcgcc atcggtggcc agtatgcgca gaaccgatac gaaacccgca
     4561 agccgggcca gcagatcgtc gtacgcctaa agagcggtgt gctggtggtc gtgacgcagg
     4621 ccgaagatcc ggcgctccgg ctcggccaga acgtctatgt acagggtgcg ggcgccgacg
     4681 cgagagtcgt tccgcagtac taactttcga gtgcccgtgg tagcgagggt gacgagggcc
     4741 gggttgcggc cgctggcgcg atcggtcgtt tcctgaagga aacttccacg gattcctgcg
     4801 ctcggccaac acccgtccgt gggtttcatt ttgtcctgct cgagggtccg atgaaagtgt
     4861 gcttccgtat gctgatgctg acttgggtga tcggggcggg tctgctgggc ctcgcggcat
     4921 gtgcgaacca gggtgccgac gcttcaacac tgcctccgcc gatggacgcg agctatgcaa
     4981 cgccggcggc tacgccgacg acggcaggca ccatggtccc ggtggtgtac gagccgaacg
     5041 cgtcgatgac agcgagcacc gcgaactaca gctcgctggc tggccaggcg gcggtcgcgg
     5101 ccccgacatc accaccaacg gcgcctcggt cggccgaccc gtggccgcgg gatgtcaccg
     5161 tttccgatgc cgatgcgctg atttaccagc cgcagatcga atcatggaca ggcaaccaga
     5221 tggcgtggcg tgtcgcggtg gcgctgcggc ccgctggcgc aaaggctgaa accttcggcg
     5281 tgttgtgggg cagcgcgcgc accgaggtcg atcgatccac gcgcaccgtc tcgctggagg
     5341 acgtcagtgt cagccagatc aagttcccca cgctgccgga caacggcgcg gcctaccttg
     5401 caggtctgcg acaggcgctg catggcgcgc tggcgaccat tgcgctcgac agcgtcgagg
     5461 cctcgctggc ggcatcgcaa tcagtcaagc cgaacggtca tgcggtgcgc aatgagccgc
     5521 caaaggtcat cgtcgcgtac gggccggcac ttctgatccc gatcgagggc cagccagtgg
     5581 tcagggcgat gagcgacgct cgcttcgagc gggtcatcaa tacgcaggcg ctgatcgcgc
     5641 gccgtcgctt tggcgatacc tggtttctca agctctatga cggctgggtg tcgtcgactt
     5701 cgctcgccgg tccgtggaca caggcgggct ttgtcccatg ggggctggag gacgcgtcga
     5761 agtcgctgat caaggcagga acggtcgatg ctctgaatgg caccggtgcg acgccgaagc
     5821 cgacgctggc gaacggcgtg ccggcgatcc atgtgacgga agtgccgacg gaactgatcg
     5881 tcttcaaggg ccagcccaat ctcgtcccga tcaccggcac tccgttgcta tgggcgacca
     5941 acagcgcgac cgacgtgctg gtcgacacga cgaacaatgc ctattacgtg ctgctgtccg
     6001 gacgctggta tcgcgcggcg ggactcgcag gcccctggtc gttcatcgcc agtaacgcgt
     6061 tgccggcatc gttcaaacag atcccggcga aaggctctcc cgcttccatc gtgctggcct
     6121 cagtggcggg cacaccgcag gcgcaggagg ccgtgatcgc caattcgatt cctcagacag
     6181 ccagcgtggc acgcgcgggt gggccgatct tcgcaccgga gttcgacggc gcgccggtgt
     6241 ggcagccggt aacgagcaca tcgctttttt acgcggccaa tgcacgtatt ccggtcatcc
     6301 aggtgaatcc gtcgagctac tacgccgtgc aggccggtgt ttggttcagc gcgcctgtgg
     6361 tgacgggtcc gtgggtggtt gcgacgacgg tgccggaagt gatctacacg atcccggtgg
     6421 cttcgccgct gcactacgtg acttatgtcc gcgtctacgg atacaaggac gacctggtct
     6481 atgtcggcta cacgccggga tacatgggca cggtcgtcga tgtcggtggc acggtggtct
     6541 acggcaccgg ctacagctac tcgccgtgga tcggcacggt ctggtacggg gcgcccgtca
     6601 cctggggcgt ggctgctgca cccgtctaca acccttatgt cggattcacg tatggattcg
     6661 gcataggact cgccacggcg gcgtgggccg agccgtactg gggcggtgcc tactatcacc
     6721 cgggctacta cggctatccg tgctgcgggt cggccagcgc caacgtgtat cgcaactggg
     6781 ggaccggcgt gtcttcggga acgcgcacct ggtacaacaa tgccgggggt tcattcggca
     6841 ccgcggccag cggttcatat gcgaccgatc gcggcacgac ggggacctac aacgcacagc
     6901 gcagctggaa tccgtatacg gggcaaggct cgcagggcta caaccgcacg gtcaataccg
     6961 cgtcaggtac gaccggctcg gtcaaccgca acgagaccta caacgcgcag accggccagc
     7021 gcggctatgg ctcgaacgtg gaggcgaccg gcgccggcgg cagctcgatc gatcgcaatg
     7081 tgtccgcgac cgccggcccc cagggtgttg accgcagcgc ctcgaccacg acctacaacg
     7141 ccaacaccgg acagaccaag acctggagcg acggcacgcc gcagaacacg cacgtcgcgg
     7201 gctcggatgg caacgtctat cgcagcgacg gcagtggcgg ttggcagcag cactcgacga
     7261 gtggctgggg cagtgcctcc ggcaactctg cgtgggccag ccaggagcag caggcgcgca
     7321 gcacggcggc cgatcgaacc aacagctacg gaggcggcgg ctgggatcgc tcggggagcg
     7381 gatcggacag cagccgctac ggtggcggag acagctgggg gaaccgcttc ggtgggagcg
     7441 ccggcggcag tggcagctgg gacaaccgat ttggcggagg cagttacagc ggtcgctttg
     7501 gcggcggcgg cttccgtggt ggtggccgca ggtagcggac gggtcgggtg cacaaggtga
     7561 cagactcatg agcgacgccg tgctgaccaa agatgaatcc gccgggcact atcgcctgat
     7621 gcgcgacggg gccgaggtcg gctacgtcga gtacgaccgt gtcggcgaat attcgatcct
     7681 gatcaagcac accgaagtcg cggcggaaca cgaagggcag gggctgggcg cactgctggt
     7741 cagcggagtg ctcgatgatg tgcgccgaca gggcaagacg gtcatcccga tctgccccta
     7801 cgcgctcaac tacatccgca agcatcgcga ttacatcgac gtggttcacg ccgacatgcg
     7861 ctcgacgatc tgactcgcgg tgtcgtcgct ggcaggcgag ccatcgcgag ccttcagcgt
     7921 gccggcgtga gcggcctccg ccgctcctat actcgatgcc cctgagcatc gaatccacat
     7981 gagcatcgaa tccactcgta acgctgagcg cgcctcggca tgagcgacct gcgagacgtc
     8041 gacattgccg tcgtcggtgc cggcgtggcc ggcatggcgt gtgcgctggg actgtcacag
     8101 cagggactgc gcgtcgcgct gatcgggccg cgggtcgtcc cgcacgcccc atcaaccgcg
     8161 gcaccgtacg atgcccgcat ctacgcggtc gccccggcca cgatcgagct gctgcaacgg
     8221 ctttcggtat ggaaccgcgt cgacgcgacg cgagccagtc cggtcgagcg catgcgcgtt
     8281 ttcggagatc gcg
//
LOCUS       DATIRT010000062         5075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_204159, whole genome shotgun sequence.
ACCESSION   DATIRT010000062 DATIRT010000000
VERSION     DATIRT010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204159"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VLN25_01530"
     CDS             <1..204
                     /locus_tag="VLN25_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09284.1"
                     /translation="AERMDGVSIHTPELDGVSGALVWAIHENEPAAGCLLRAAAVQVG
                     FAHDRYVRTESLRSAPVLFRRFR"
     gene            complement(282..575)
                     /locus_tag="VLN25_01535"
     CDS             complement(282..575)
                     /locus_tag="VLN25_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09285.1"
                     /translation="MQIPTTPAKPNRARRRAHRTLAGAVFGASATFLVLLGATAVSDS
                     PRQLSDIPETLAAAEVFDGPPLPAFETGGDVALTPTPRRYDEPGLRVNVEVPA"
     gene            complement(757..1341)
                     /locus_tag="VLN25_01540"
     CDS             complement(757..1341)
                     /locus_tag="VLN25_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HSQ09286.1"
                     /translation="MAYAPEYVPKILIIDSQGQPVRWALMQRAARYYASGKVVTDLGD
                     NLFGMAGGLQEATGLRSEFVTSSIVMIRGRHRIPFGHAHVGLTKHRLFARDRQVCAYC
                     GGHFVELDLTVEHILPVSRGGRHEWTNVVTACRSCNTRKGSRRPEEANMPLLYVPYAV
                     CRNEGFILSNRRILADQMMFLQASLPRHSRWAHG"
     gene            complement(1397..1660)
                     /locus_tag="VLN25_01545"
     CDS             complement(1397..1660)
                     /locus_tag="VLN25_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09287.1"
                     /translation="MESHAPRSSPPLSLQDKLDCITHERKVLEILRELMRHGLHDGDD
                     VQHRADGSHGRVVVLRSAGEPHSVVQLDDGTQIAFHAADWGRP"
     gene            complement(1742..2233)
                     /locus_tag="VLN25_01550"
     CDS             complement(1742..2233)
                     /locus_tag="VLN25_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09288.1"
                     /translation="MLCRILPGLLLASTLAACGMPGSVRPGADQPSVRSTAGTPTATM
                     TLPGGGTRWQYSTQPNSQFVWNIDFDAQGRVVRTEQMMSDEVFARIRSGRDTRADILR
                     EFGPPAETYTFHLKDETAFMYRYFIQGGFYAAMFIYFNPAGVVDRTETGMDPWRIRDG
                     DRR"
     gene            2294..2824
                     /locus_tag="VLN25_01555"
     CDS             2294..2824
                     /locus_tag="VLN25_01555"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSQ09289.1"
                     /translation="MKWLRRIVVAVAAVIALLLVIGMLLPSGFKVQRSVQVAAPPSKI
                     YPLIVDPRQWKNWTIWNQRDPAMQMQYSGAETGVGAKWSWQSKTEGNGAMEFTAAVPN
                     ERVVYSLSFPEMGMTSRGELQLVPDGAGTRVTIMNEGDMGSSPVNRYFGLMMDSLVGP
                     DFEGGLKNLKALAERG"
     gene            2877..4094
                     /locus_tag="VLN25_01560"
     CDS             2877..4094
                     /locus_tag="VLN25_01560"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSQ09290.1"
                     /translation="MSTLATRALVATTGIQAAAACAALTIPSISPVIAAQLDVPASMV
                     GTYISLLYIGASTAALAGGGVVLRYGAMRLSQACLLVCGAGLLLTLFLGVGAGPLALV
                     AIALSAVAIGVGYGPITPASSHVLAKTTPRERMALMFSIKQTGVPAGTALAGLIVPPL
                     TIAFGWQVAVVLIALGCIVVAVASQPLRAPLDDDRDRSARLSAQSLIGGLSLVAHAPA
                     LRTMALVSFIYAGMQMSVAGFIVAYLHTEIGLGLVAAGTALTTASVAGVVARISWGSI
                     SDRTGAPRTTLAVIGFLMAGASVAAALFTPSWPMLAILTVSAALGATAIGWNGVFLSE
                     VARLAPAGEAGRATGGCLFFTYVGVVILPFLFGWLQRASGSYALCFAVAAGVCTAVAV
                     MLLWTGRRPRAQG"
     gene            4186..4536
                     /locus_tag="VLN25_01565"
     CDS             4186..4536
                     /locus_tag="VLN25_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09291.1"
                     /translation="MRRPSLLVTLLIPALAAATTAALGPFGLEVPARGFDEHCIRLDA
                     GEHVRYRFSANGEVDFNIHYHRGSEVYYPVKAAASRSADATFTAPHADTYCLMWERKG
                     EGAVRVDGSVERAR"
     gene            4582..>5075
                     /locus_tag="VLN25_01570"
     CDS             4582..>5075
                     /locus_tag="VLN25_01570"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HSQ09292.1"
                     /translation="MPSHRPTHKPAQNAAREATDEIELKLHLPDGAIEALLLNPLLKD
                     SPPRTTRLDATYFDTADRLLQQHGMALRLRRSGRQWLQTLKAVGTARGGLSARLEWEF
                     PAKLIRGAPRIDLALLNDSPLPALLSRHKKSGSLQPVFKVRVTRTLWDVSYKRSRFEV
                     AMDRG"
BASE COUNT          822 a         1582 c         1781 g          890 t
ORIGIN      
        1 gccgagcgca tggacggggt gtcaatccat acgccggaac tggacggcgt ttcgggtgcg
       61 ctggtctggg ccattcacga gaacgagccc gcggccggct gcctgctgcg tgcggcggcc
      121 gtgcaggtcg gatttgcgca cgatcgatat gtgcggaccg agtcactacg ctcggcgccg
      181 gtgctcttca ggcggttccg ctagcgcagc tgcgcgcctc gctcgactca cgtcgctcga
      241 cgtccccgcc gtttgcccgt gccgcagtgt gcggcggtgg ctcaggcagg tacttcgaca
      301 ttcacccgca gccccggttc gtcgtaacgt cggggagtcg gcgtgagggc gacgtcacct
      361 ccggtttcga aggccggtag aggcggcccg tcgaacacct cggctgccgc cagcgtttcg
      421 gggatgtccg acagttgtcg cggactgtcc gagacggccg tcgcgcccag aaggacaagg
      481 aaggttgcgg atgcgccgaa cacggcgccg gcaagcgtgc ggtgggcgcg ccgccgggcc
      541 ctgttgggct tcgcgggagt tgtcgggatc tgcatgtcga gtctccaatg acgggctgtg
      601 cgcgtcacag cgcggcgtcg atgcgagctg catcggatga ctcgtacagt acgaccgtga
      661 tgtcgcgacg acgaccgacg aaacggtacg cgcgacgtgc actattcgac agtcgcgtgc
      721 aaccggttca ggacaagctg cggacgcagg cggtgatcag ccgtgcgccc agcgcgagtg
      781 ccgtggcagc gacgcctgga ggaacatcat ctgatcggcg aggatacggc gattgctgag
      841 aatgaagcct tcattgcggc agacggcgta aggcacgtag agcagcggca tgtttgcttc
      901 ttcgggccgt cggctgccct tgcgcgtgtt gcacgaacgg caggcggtga cgacgttggt
      961 ccactcatgc cggccgccgc gcgacaccgg caggatgtgt tccacggtga ggtcgagttc
     1021 cacgaagtgc ccgccgcagt aggcgcagac ctgccggtcg cgggcgaaca gccgatgctt
     1081 ggtgaggccg acatgcgcgt gaccgaacgg aatacggtgg cgcccgcgga tcatcacgat
     1141 cgagctcgtg acgaattcgg agcgcaggcc cgtggcctcc tgcagtccac cggccatgcc
     1201 gaagaggttg tcaccgagat cggtgacgac cttgcctgac gcgtagtagc gcgcagctcg
     1261 ctgcatcaac gcccaacgga ccggctggcc ctgggagtcg atgatcagga tcttcggtac
     1321 gtactccggc gcataagcca tgatgccgcg aattgtgcca cgcggccggc tcgtgcggcg
     1381 tgagggcgaa ttacggctac ggccgacccc agtcagcggc gtggaaagcg atctgcgtgc
     1441 cgtcgtcgag ctgcaccacc gagtgcggct cgccggccga tcgcagcacg accacgcgcc
     1501 cgtgcgaacc gtcggcacgg tgctggacgt cgtcgccatc gtgcagcccg tgacgcatca
     1561 gctcccgcag gatctccagg accttgcgct catgcgtgat gcagtcgagc ttgtcctgta
     1621 gggacagcgg aggcgaactg cgcggtgcgt gggattccat tgcgagaccg gaacgacgtg
     1681 acgcggcatt ctgggcggac gtcgcgggcc gcggaattcg gtcttacccg gggcagacgc
     1741 attagcggcg atcgccgtcg cgaatccgcc acgggtccat gccggtctcg gtcctgtcca
     1801 cgacgccagc cgggttgaag tagatgaaca tcgccgcgta gaacccgccc tgaatgaaat
     1861 agcggtacat gaacgccgtc tcgtccttca ggtggaacgt gtaggtctcg gcaggcgggc
     1921 cgaattcacg caggatgtcg gcgcgggtgt cgcgcccgct tctgatgcgc gcaaacactt
     1981 cgtcgctcat catctgctca gtgcgcacga cgcggccctg ggcatcgaag tcgatgttcc
     2041 agacgaactg gctgttcggc tgggtggagt actgccagcg tgtgccaccg ccgggcagtg
     2101 tcatcgtggc ggtcggcgtg cctgcggtcg agcgcaccga aggttggtct gcaccggggc
     2161 gaaccgagcc cggcatgcca caggcggcga gggtggacgc cagcaggagt cccggcagaa
     2221 tgcgacaaag cataggcaca atcctttcgc gcgcggctac gctgcgcacc tatattaggt
     2281 cagggaggtg accatgaaat ggctcaggcg catcgtggtg gcggtggcag cggtcatcgc
     2341 gctgctgctg gtcatcggca tgctgttgcc gtccggattc aaggtgcagc gatcggtaca
     2401 ggttgcggcc ccaccgtcca agatataccc gttgatcgtg gatcctcgcc agtggaagaa
     2461 ctggaccatc tggaaccagc gcgacccggc gatgcagatg cagtactccg gcgcggaaac
     2521 cggggtgggc gcgaagtgga gctggcagag caagaccgag ggcaacggcg cgatggaatt
     2581 cacggcggcg gtaccgaacg agcgggttgt ctattcgctg tcgtttccgg agatgggcat
     2641 gacgtccagg ggcgaattgc agttggtacc cgacggcgcc ggcacgcgcg tcaccataat
     2701 gaacgaaggc gacatgggca gcagtcccgt caaccgctac ttcggcctga tgatggactc
     2761 cctcgtcggc ccggacttcg agggtggact gaagaacctg aaggccctgg ccgagcgcgg
     2821 ttaggcaggc agtccgcctc gcgcccgcac gaaggcgccg gcacgctgag gcgtgagtga
     2881 gcacgctggc cactcgcgca ctggtcgcga cgacaggaat ccaggccgcg gcagcgtgcg
     2941 cggcgctcac gatcccgtcg atctccccgg tgatcgccgc gcaactggac gtgccggcgt
     3001 ccatggtcgg cacctacatc tcgttgctgt acatcggtgc gtcgacggcc gcgctggcag
     3061 gtgggggtgt cgtgctgcgt tacggcgcga tgcggctgtc gcaggcctgc ctgctggtct
     3121 gtggcgctgg gctgctgctg acactgtttc tcggcgtcgg tgcgggaccc ctggccctcg
     3181 tggcgatcgc attgtcggcc gtggcgattg gcgtgggtta cggcccgatc acgcccgctt
     3241 cgagccacgt gttggcgaag acgacgccac gcgaacgcat ggcgctgatg ttctcgatca
     3301 agcagacggg cgtgccggcg ggaacggccc tggcggggct gatcgttccg ccgctgacca
     3361 tcgcattcgg gtggcaggtc gcggtggtcc tgatcgccct ggggtgcatc gtcgttgccg
     3421 tggcatcgca accgctgcgc gcgccgctgg atgatgatcg cgatcgctcc gcgcgactgt
     3481 cggcgcaatc gctgatagga ggcctctcgc tggtggcgca cgcgccggcg cttcggacga
     3541 tggcgctagt gtcgttcatc tatgcgggca tgcagatgtc ggtggcggga ttcatcgtcg
     3601 cctatctgca caccgaaatc gggttggggc tggtggcggc gggaaccgca ctgaccaccg
     3661 cgagcgtggc cggcgtggtg gcgcgcatca gttggggctc gatctcggac cgcaccgggg
     3721 cgccgcgcac gacactggcc gtgatcggtt tcctgatggc gggcgcgtcg gtcgccgcgg
     3781 cgctgttcac gccatcctgg cccatgctgg cgatactgac agtctccgcc gcgctcggcg
     3841 cgacggcgat cggctggaac ggtgtcttcc tctcggaagt ggcgcggctg gctcctgcag
     3901 gagaggccgg gcgggcgacc ggaggatgcc tgttcttcac ctatgtcggc gtcgtgatcc
     3961 tgccgttcct gttcggctgg ttgcaacgtg ccagcggcag ctacgcgctg tgctttgctg
     4021 tggcggccgg cgtgtgtact gcggtcgcgg taatgctgct ctggacagga cgcaggccgc
     4081 gcgcgcaagg ctgatctggc ccctgttccg cgctgcggat tcgtgtcatc gggctgcatg
     4141 cgacgaaccc agcaagcaat ggagcctgca tgcgatgatc gcctcatgcg acgtccctcg
     4201 ttacttgtca ccctgctgat tcccgccctc gcagcggcaa cgaccgccgc gctgggaccg
     4261 ttcggtttgg aggttccggc gcgcggcttt gacgagcatt gcattcgtct tgacgccggc
     4321 gagcacgtcc gataccggtt cagcgcaaac ggcgaggtgg acttcaatat ccactatcac
     4381 cgcggcagtg aggtctacta cccggtcaag gcggccgcaa gccgatcggc cgatgcgacg
     4441 ttcaccgcac cgcacgccga cacgtactgc ctgatgtggg aacgcaaagg cgaaggtgcg
     4501 gtgcgggtcg acggatcggt cgagcgcgcg cgctagcccg gtgtcacgaa agtcgtggcg
     4561 cgcggccgat agactgcagt gatgccgtcg cacagaccca cgcacaaacc tgcgcaaaac
     4621 gccgcgcgcg aagcgacgga cgagatcgag ctcaagctcc atctgccgga cggagccatc
     4681 gaggcactgc tactcaaccc gctgctgaag gactctccac cccgtaccac gcggctcgat
     4741 gcgacttact ttgacaccgc cgatcggctg ctgcagcagc atggcatggc gctgcgactg
     4801 cgtcgttccg gccggcagtg gttacagacg ctgaaggccg tcggcacggc gcgtggcgga
     4861 ctgagcgcgc ggctcgagtg ggagttcccc gcgaagctca tcaggggggc accacggatc
     4921 gatcttgcgt tgctgaatga ctcgccgtta cccgccttgc tgtcgaggca caagaagtcc
     4981 gggtcgttgc aacctgtctt caaagtccgg gtaacgcgca cgctgtggga tgtcagctac
     5041 aagcgcagcc ggttcgaagt ggcgatggat cgcgg
//
LOCUS       DATIRT010000063         3588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1089196, whole genome shotgun sequence.
ACCESSION   DATIRT010000063 DATIRT010000000
VERSION     DATIRT010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089196"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1263)
                     /locus_tag="VLN25_01575"
     CDS             complement(<1..1263)
                     /locus_tag="VLN25_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HSQ09293.1"
                     /translation="MTSGRTTKRAGRDSLSSLSRLIALACAVTIALPPSIPAAIAQTK
                     APAKGKATAASGAAAADSVMLNFVNAEIDAVVRAIGQYTERTFVIDPRVKGTLSLVTE
                     RPVTKQQAFDQLLSALRLQGFTIVQTGNVMRVVPEADAKLQGGTVVAPRSQNPGGDQL
                     VTQVFRLQYESATAMVPILRPLIAPNNTISAYPQNNTLVITDYAENLRRIQRIIESID
                     TPATSDLEMIPVKFGLAMEIATVVNRVLDEGARAAGQAVDAGQRVSVLAEPRTNTLIV
                     RAPSQARLTLAKNLIAQLDRPASTPGNINVVYLRNAEAARLAPLLRAIIGSDPSYVPQ
                     AASSAGLSQQSSTSGAPGQPGGAPGGGMTAGSTAAVGSSGGGGGALAGLIQADAATNS
                     LIITAPEPLYRNIRAIIDKLDVRRAQVVI"
     gene            complement(1265..2044)
                     /gene="gspN"
                     /locus_tag="VLN25_01580"
     CDS             complement(1265..2044)
                     /gene="gspN"
                     /locus_tag="VLN25_01580"
                     /inference="COORDINATES: protein motif:HMM:NF013376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HSQ09294.1"
                     /translation="MRTALYLLAALVVLTLTMLIIAPAQWAASAVRSATSGRVDLAEA
                     RGTVWNGSAVLVLAAAADASATRASLPERLSWQLSPWSLLAGQINLTIAHPSALTQPL
                     NIRAPIFGGTTMLGATTVRLPASLLVGIGAPWNTVRPGGILTLSWDRLQIEPGRMLGN
                     LSAEWQYASSALTPVSPMGHYRLQTNGVWPGTQLELLTISGPLELRGSGTIPEGGRLR
                     FTGRAQALAGTDPGVKAQLTGLISLLGRRDGDGALLNFGGG"
     gene            complement(2054..2545)
                     /locus_tag="VLN25_01585"
     CDS             complement(2054..2545)
                     /locus_tag="VLN25_01585"
                     /inference="COORDINATES: protein motif:HMM:NF016493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein M"
                     /protein_id="HSQ09295.1"
                     /translation="MDALKQFWAERAPRERAILGFGASVVLIAVLYLMLIEPAWTGIG
                     RLERSLPQQRSLAAELDALLGEVRGLKSQPQVATVSPSEVRGAIETSLTRAGMKAARI
                     VPLSDGDIQLTFANVPYGTWAAWLAGIERELGARTTSVVITGKDATPGNVDAELALRL
                     ARK"
     gene            complement(2545..>3588)
                     /gene="gspL"
                     /locus_tag="VLN25_01590"
     CDS             complement(2545..>3588)
                     /gene="gspL"
                     /locus_tag="VLN25_01590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HSQ09296.1"
                     /translation="GKLRLALPNILEDRLLADATDCHFAFNIPRGGSGTTTIAALPKM
                     PVAVIDRGVLTRALDACAEAGYRVRAAYSEIYTVPPPAAGVLSVRVDRGRGIARSATH
                     DGFSFELAGDEAPAALALAVRQLGVRSIKAYGREAARISPLATGLGVQIDIVPSDVDT
                     VSADNAVNLLQGSFAQGGMLSTLMPLAGRAAALRGVSFKAPLIWTAVGAIVALVGMNA
                     YWLKLDAEAKALKQQMENAFRASFPETTAVVDPVLQTKRQLNDLRARAGLPSANDFSV
                     LNARTALLLSAAPLGSVGGLEYRDGALKVKFKGGMADNPAFQNTLRSAAVQQGLTIRF
                     EADGTARVTAAGS"
BASE COUNT          563 a         1182 c         1279 g          564 t
ORIGIN      
        1 gatgacgacc tgcgcgcggc gcacgtcgag cttgtcgatg atcgcgcgga tgttgcggta
       61 aagcggttca ggggccgtga tgatcagcga gttggtcgca gcatcggcct gaatcaggcc
      121 ggccagcgcg ccacccccgc cgccggagct gccgaccgcc gccgtgctgc cggctgtcat
      181 accgccgcct ggagcgccgc ccggttggcc cggcgcgccc gatgtcgaac tctgttgcga
      241 caggccggcc gagctcgcgg cctgcggcac ataggacgga tccgagccga tgatggcacg
      301 cagcagcggc gccaggcgcg cggcctcggc gttgcgcagg tacacgacat tgatgttgcc
      361 gggcgtcgag gccggacggt cgagctgtgc gatcagattt ttcgcgagcg tgaggcgcgc
      421 ctggctggga gcgcgcacga tcagcgtgtt cgtgcgcggc tcagccaaaa ccgaaacgcg
      481 ctgaccggca tcgaccgcct gaccggccgc gcgcgcgcct tcgtcgagca cgcggttgac
      541 gaccgtggcg atctccatcg ccaagccgaa tttcaccggg atcatctcga ggtcggaggt
      601 ggccggcgta tcgatcgact cgatgatgcg ctggatgcga cgcaggtttt cggcgtaatc
      661 cgtgatgacc agcgtgttgt tctgcgggta ggccgagatc gtgttgttcg gcgcaatcag
      721 cggccgcagg atcggcacca tcgcggtggc cgactcgtac tgcagacgga acacctgggt
      781 gacgagctgg tcgcccccgg gattctggct gcggggcgcc acgaccgtgc caccctgaag
      841 cttagcgtcg gcttccggta ccacgcgcat cacgttgccg gtctgcacga tcgtaaagcc
      901 ctgaagtcgc agggcagaca gcagctggtc gaacgcctgc tgcttggtca cgggccgctc
      961 ggtcaccagc gacagggtgc ctttgacgcg cggatcgatg acaaacgtgc gttccgtgta
     1021 ctggccgatg gcgcgcacca cggcatcgat ctcggcattg acgaagttaa gcatcacgct
     1081 gtcagccgcg gccgcgccgc tggcggcggt ggccttgccc ttcgccggcg ccttggtctg
     1141 cgcgatggcg gccgggatcg agggcggcag cgcgatggtg acggcacagg ccagcgcgat
     1201 caggcgggac agcgaggaca gtgagtccct ccctgcgcgt tttgttgtgc gcccgcttgt
     1261 catgtcaccc gccgccgaag ttcaggaggg ccccatcgcc atcgcgccgc cccagcagcg
     1321 agatgagacc cgtcagttgt gccttcacac ccgggtcggt gcccgccaag gcctgtgcgc
     1381 ggcctgtaaa gcgtaagcgc ccaccctccg ggattgtgcc actccccctc agctcgaggg
     1441 gacccgaaat ggttagtaat tccagctgag ttccgggcca gacgccgttg gtctgcaggc
     1501 ggtagtggcc catgggcgac accggggtca gcgcgctcga cgcatactgc cactcggccg
     1561 acagattgcc gagcatgcgg cccggctcga tctgcagccg gtcccacgac agggtcagga
     1621 tgccgcccgg ccgtaccgtg ttccagggag cgccgatgcc caccagcaat gaagccggaa
     1681 gtcgcacggt cgtcgcgcca agcatggtgg tgccgccgaa aatgggtgcg cggatgttga
     1741 gcggctgcgt cagcgccgat gggtgcgcga tggtcaggtt gatctggccg gcgagcagcg
     1801 accaagggct caattgccag gacaggcgct ccggcagact cgcgcgcgtc gcgctggcat
     1861 cggcagcggc cgcgagcacg aggacggccg aaccgttcca gaccgttccg cgggcttcgg
     1921 ccaggtcgac ccgacccgag gtggcggacc gcacagcgct ggcggcccat tgtgcgggcg
     1981 cgatgatgag catcgtcaac gtcaggacga cgagcgcagc cagcaggtaa agggcggttc
     2041 gcatcgccgg gcatcatttg cgcgccaggc gcagcgcaag ttcggcatcg acgttgccgg
     2101 gcgtggcgtc cttgccggtg atgacgaccg acgtcgtacg ggctccgagt tcgcgctcga
     2161 tgcccgcgag ccacgcggcc caggttccgt acggaacgtt ggcaaaagtc agctggatat
     2221 cgccgtcgga caacgggacg atgcgtgctg ccttcatgcc ggcgcgcgtc agcgatgtct
     2281 cgatggcgcc gcggacctcg gagggcgaga ctgtcgccac ctgcggctgc gacttgaggc
     2341 cccggacttc accgagcaga gcgtcgagtt cggctgccag cgatcgttgc tgcggcagcg
     2401 agcgttcgag ccggccgatg ccggtccagg ccggttcgat cagcatgagg tacagcaccg
     2461 cgatcagcac cacgctggcg ccaaaaccaa ggatggcgcg ctcgcgggga gcgcgctcgg
     2521 cccagaactg cttcagtgcg tccatcagga gcccgccgcc gtgacccgcg ccgtgccgtc
     2581 ggcctcgaag cggatcgtca ggccctgctg taccgcggcc gagcgcagcg tgttctgaaa
     2641 cgcggggttg tcggccatgc cgcccttgaa cttcaccttg agtgcgccat cgcggtattc
     2701 gagtcccccg acgctgccga gcggcgcggc gctcagcagc agggccgtgc gtgcattgag
     2761 gaccgagaaa tcgttggcgg acggcagccc cgcgcgggcg cgcagatcgt tgagctgtcg
     2821 cttcgtctgc agcaccgggt cgacgacggc ggtggtctcc gggaagctgg cgcggaatgc
     2881 gttctccatc tgctgcttca gggccttggc ctcggcgtcg agcttcagcc agtaggcatt
     2941 catcccgacg agggcgacga tcgcgccgac cgccgtccag atcagcgggg ccttgaagga
     3001 cacgccacgc agcgcggcgg cgcgaccggc cagtggcatc agggtgctga gcatgccgcc
     3061 ctgcgcgaag ctgccctgca acaagttgac ggcgttgtcg gcgctgacgg tgtccacgtc
     3121 gctgggcacg atgtcgatct gcacgccgag tccggtcgcc agcgggctga tgcgcgccgc
     3181 ttcgcgcccg taggccttga tgctgcgcac gcccagctga cgcacagcga gcgcgagcgc
     3241 ggcgggcgcc tcgtcgcccg cgagttcgaa ggaaaaaccg tcgtgggtcg ccgagcgagc
     3301 gatgccgcga ccgcggtcga cacggaccga cagcacgccg gccgctggcg gcggcaccgt
     3361 gtagatctcg ctgtaggcgg cgcgcacgcg atagccggct tcggcgcagg cgtcgagtgc
     3421 gcgtgtcagg acgccgcgat caatgacggc caccggcatc ttcggcagcg cggcgatcgt
     3481 ggtggtcccg gatccgccgc gcggaatgtt gaaggcaaag tggcagtcgg tggcatccgc
     3541 cagcaggcgg tcctccagga tgttgggcag ggcaaggcgc agcttgcc
//
LOCUS       DATIRT010000064         4185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1187680, whole genome shotgun sequence.
ACCESSION   DATIRT010000064 DATIRT010000000
VERSION     DATIRT010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187680"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VLN25_01595"
     CDS             complement(<1..269)
                     /locus_tag="VLN25_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HSQ09297.1"
                     /translation="MNDVGASGFTSAFWDYYVAILTIVSVLGCAVFLWSQSGRTVKAK
                     VNAKGEIETTGHVWDGDLQEYHNPMPRWWIWLFYLTVLFSVIYLIL"
     gene            complement(266..442)
                     /locus_tag="VLN25_01600"
     CDS             complement(266..442)
                     /locus_tag="VLN25_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003087298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HSQ09298.1"
                     /translation="MDLNDARALLTVLGFIGFIAIVAWAYSKGAKKGFKEASELPFAD
                     RDEAQRSGEQGNKK"
     gene            complement(456..1070)
                     /gene="ccoO"
                     /locus_tag="VLN25_01605"
     CDS             complement(456..1070)
                     /gene="ccoO"
                     /locus_tag="VLN25_01605"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HSQ09299.1"
                     /translation="MKLTHDKIEQSVPLLIVLVILVIAVGGLVEIVPLYFQREVTQPV
                     KGIKPYEPLRLVGRDIYIREGCYNCHSQMIRPFRAETLRYGHYSVAGEFVYDHPFQWG
                     SKRTGPDLARVGARYSDEWHRIHFNNPRDVVPESNMPAYPWFEKNKLDGSDIQPRMKA
                     LRSIGVPYTDDQIAKAPEALKGKTELDAVIAYLQGLGVELKGLR"
     gene            complement(1111..2544)
                     /gene="ccoN"
                     /locus_tag="VLN25_01610"
     CDS             complement(1111..2544)
                     /gene="ccoN"
                     /locus_tag="VLN25_01610"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HSQ09300.1"
                     /translation="MNSAVTADTYNYKVVRQFAVMTVIWGIVGMLVGVIIAAQMSWPE
                     LNFGIPWLSFGRLRPLHTNAVIFAFGGCALFATSYYVVQRTSQARLWGGPLVAFTFWG
                     WQLVIVAAAITLPLGITTSKEYAELEWPIDVLITLVWVAYAVVFFGTIVKRKVKHIYV
                     ANWFYGAYIITVAVLHLVNSAAVPAGLWKSYSVYPGATDAMVQWWYGHNAVGFFLTAG
                     FLGIMYYFVPKAAGRPMFSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWAQSIGMVF
                     SLILLAPSWGGMINGIMTLSGAWHKLRDDPILKFLIVSLSFYGMSTFEGPMMSIKTVN
                     ALSHYTDWTIGHVHSGALGWVGLISIGAIYYLLPRLYGRTEMYSMRLVTTHFWVATIG
                     IVVYIAAMWIAGVMQGLMWRAVNDDGTLTYTFIESVKATYPFYVIRLLGGLLYLGGMF
                     LMAWNMVKTIAAGKAVEAPIPAAMAHA"
     gene            complement(2845..3027)
                     /gene="ccoS"
                     /locus_tag="VLN25_01615"
     CDS             complement(2845..3027)
                     /gene="ccoS"
                     /locus_tag="VLN25_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HSQ09301.1"
                     /translation="MDSLYLLIPLSVLLILAIAAILVWAVTSGQFERLDDVGARVLRE
                     DPDTDADAAHRSAPRT"
     gene            complement(3035..>4185)
                     /locus_tag="VLN25_01620"
     CDS             complement(3035..>4185)
                     /locus_tag="VLN25_01620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HSQ09302.1"
                     /translation="LTVVGWWLVDASQALPNAIAVLVATCPCALSLAAPAAFSAATSA
                     LAQRRVLLARTAALETLAEVDVLACDKTGTLTTGDPALLKQLLLRDVDPERMLAVAAA
                     METMSTHPYARALLQAAQAMPVTLPAVTEGRVEASAGIEATIGSQRYRLGKIEFALPE
                     KLSPQRAGVAAIVAQEGLAAASYVVLADDAGALAVFAFGERLRDDAAALVDAAADRDV
                     EVVLLSGDRREPVQAVARSLGIERALAHQTPDSKRHWVAGQQGAGHRVAMLGDGMNDA
                     PVIAQADVSIALAGGSMLAQARADFIVLNSRLADVEFALAVAHRARRIVRQNLAWAFV
                     YNVVIIPLAAFGYVSPAVAAAGMALSSLAVIANALRAKNVPTGGAFPR"
BASE COUNT          802 a         1372 c         1328 g          683 t
ORIGIN      
        1 aggatcaggt aaatgacgga aaagagcacg gtcaggtaga acagccagat ccaccagcgc
       61 ggcatcgggt tgtgatactc ctgcaggtca ccgtcccaga catggccggt ggtctcgatc
      121 tcgcccttgg cgttgacctt agccttgacc gtccggccgc tttgcgacca cagaaacacc
      181 gcgcagccga gcaccgacac gatcgtgagg atcgcgacgt agtagtccca gaaggcgctg
      241 gtgaagccgc tcgctccgac gtcattcatt ttttgttccc ctgttcaccg ctgcgctgcg
      301 cttcgtcgcg gtcagcgaac ggcagctccg atgcttcctt gaatcccttc tttgcaccct
      361 tgctgtaggc ccacgccacg atggcgatga agccgatgaa ccccaaaacg gtcagcaggg
      421 cgcgggcgtc gttcagatcc atggtccgcc gcctgctacc gcagtccctt cagttcgacg
      481 ccgagtccct gcaggtaggc gatcaccgcg tcgagctcgg tcttgccttt gagggcttcg
      541 ggcgccttcg cgatctgatc gtcggtatag ggcacgccga tgctgcgcag cgccttcatg
      601 cgtggctgta tatcggagcc gtcgagcttg ttcttctcga accacggata ggcgggcatg
      661 ttcgattccg gcaccacgtc gcgaggattg ttgaagtgga tgcggtgcca ctcgtccgag
      721 tagcgggccc ccacgcgcgc cagatcagga ccggtgcgct tgctacccca ctggaacgga
      781 tggtcgtaga cgaattcgcc cgccaccgag tagtgcccgt agcgcagcgt ttcggcacgg
      841 aacggccgga tcatctgcga atgacagttg taacagcctt cgcggatgta gatgtcgcgg
      901 cccaccaggc gcagcggctc gtacggcttg atccccttga ccggttgcgt gacctcgcgc
      961 tggaaataca gcggaacgat ctcgaccagg ccaccgacgg cgatgacaag gatgacgagg
     1021 acgatcagca acggaacact ctgctcgatc ttgtcgtggg tgagtttcat cgtgattcct
     1081 aggtttgctc gggttccggt gcgcaccgga tcaggcgtgt gccatcgctg cggggatcgg
     1141 cgcctcgacc gccttgcccg ctgcaatcgt cttcaccatg ttccaggcca tcaggaacat
     1201 gcccccgagg tacagcaggc ctccgagcag gcggatcacg tagaacggat aggtcgcctt
     1261 cacgctctcg atgaacgtgt aggtcaacgt gccgtcgtcg ttcaccgctc gccacatcag
     1321 gccctgcatc acgccggcga tccacatggc ggcgatgtag accacgatgc cgatcgtggc
     1381 gacccagaag tgcgtggtga ccagccgcat gctgtacatc tcggtgcgcc catacagccg
     1441 cggcagcagg tagtagatcg cgccgatcga gatcaggccg acccagccga gcgcgcccga
     1501 atgcacgtgg ccgatggtcc agtccgtgta gtggctgagc gcattgacgg tcttgatcga
     1561 catcatcggc ccttcaaagg tcgacatgcc gtagaacgac agcgagacga tcaggaactt
     1621 gaggatcggg tcgtcgcgca gtttgtgcca cgcgcccgac aacgtcatga tgccgttgat
     1681 catgccgccc cacgacggtg cgagcagaat cagcgagaag accatgccga tcgactgcgc
     1741 ccagtcgggc agcgccgtgt agtgcaggtg atgcgggccg gcccacatgt aagtgaagat
     1801 cagcgcccag aagtgcacca ccgacagccg gtaagagaac ataggtcggc ccgccgcctt
     1861 ggggacgaag taatacatga tcccgaggaa gcccgcagtc aggaagaagc ccaccgcgtt
     1921 gtggccgtac caccactgca ccatcgcgtc ggtcgcgccc gggtagaccg agtacgactt
     1981 ccacaacccc gccggcacgg cggcgctgtt gaccaggtgc agcacggcga cggtgatgat
     2041 ataggcgccg tagaaccagt tggccacgta gatgtgcttg accttgcgct tgacgatcgt
     2101 gccgaagaac acgacggcat aggcgaccca gacgagcgtg atcaggacat cgatcggcca
     2161 ctcgagttcg gcatattcct tggacgtcgt gatgccgagc ggcaaggtaa tggcggccgc
     2221 cacgatcacc agctgccagc cccagaaggt gaacgccacc agcggcccgc cccagagtcg
     2281 cgcctgcgac gtgcgctgga cgacgtaata gctcgtggca aacagcgcgc acccgccgaa
     2341 ggcgaagatc accgcgttcg tgtgaagcgg acgcagtcgg ccgaagctga gccagggaat
     2401 cccgaaattg agttcgggcc agctcatctg ggcggcgatg atgacgccga ccagcatgcc
     2461 gacgatgccc cagatcaccg tcatcacggc gaactgccgc acgactttgt agttataggt
     2521 gtccgcagtc acggctgagt tcatgtagtc ctcgtgcaga aaaacgtgtg cgaccgcgcg
     2581 aactggcgca tgcgcgggcc ggcgtgaaat gggctgtctg ccttcttgtc tgcacggccg
     2641 acgcggcgac cgtgctccgg tactcgggtc gcatgctaca aaacccgctg ctgcatcgcg
     2701 attgacaaag gtcaaccgca acagaacgac cgcgccgcag cgactgaatt gtcgcggccc
     2761 ggccagacga taccctccaa agcaacgata cgggacccca ttcgggtgga ccgggctgcc
     2821 attgtcctgc aggtgcgggg cgaatcacgt gcgcggtgcc gaccgatgag cggcgtcggc
     2881 atcggtatcc ggatcttcgc gcaacacccg ggcgccgaca tcgtccagcc gctcgaattg
     2941 cccggaggtc accgcccaca ccaggatggc ggcgatcgcc aggatcagca ggaccgacag
     3001 cgggatcagg agatacaacg aatccatccg gcattcagcg cgggaacgcg ccaccggtgg
     3061 ggacattctt tgcgcggagg gcgttggcaa tgaccgccag cgaactgagc gccatgcctg
     3121 ctgcggccac tgccggcgag acgtatccga acgcggccag cgggatgatg acgacgttgt
     3181 agacaaaggc ccacgccagg ttctggcgga cgatgcgacg cgcgcgatgc gcgaccgcca
     3241 gcgcgaactc gacatcggcc agcctcgaat tgagcacgat gaagtcggcg cgcgcctgcg
     3301 ccagcatcga gccgccagcc agcgcaatcg acacatcggc ctgagcgatc acgggtgcat
     3361 cgttcatgcc gtcgccgagc atcgccacgc gatggcccgc gccctgctgc cccgcgaccc
     3421 agtggcgttt gctgtcgggg gtctggtggg cgagcgcgcg ctcgataccg agtgatcgcg
     3481 ccacggcctg caccggctcg cgccggtcgc ccgacagcaa cacgacctcg acatcgcgat
     3541 cggccgccgc atcgaccagc gccgccgcat cgtcacgcag gcgttcgccg aaagcgaaca
     3601 ccgcgagcgc acccgcgtcg tcggcgagaa cgacgtagct cgctgcagcc aggccctcct
     3661 gcgcgacgat cgcggcaaca cccgcgcgct gtggcgacag cttctcgggc aatgcgaact
     3721 cgatcttgcc caggcggtaa cgttgcgagc cgatcgttgc ctcgattccg gccgaggcct
     3781 cgacccgccc ctcggtcacg gccggcagcg tgaccggcat tgcctgtgcg gcctgcagca
     3841 gggcgcgggc gtacggatgc gtcgacatgg tctccatcgc cgcggcaacc gccagcatgc
     3901 gctcgggatc gacgtcgcgc agcagcagct gtttgagcaa ggccgggtcg ccagtcgtca
     3961 gcgtgccggt cttgtcacat gcaagcacat caacttcggc cagggtctcg agggctgccg
     4021 tccgcgcgag cagcacgcgc cgctgcgcca gggccgacgt tgccgccgag aacgccgcgg
     4081 gcgccgccag ggacaacgca caggggcagg tcgcgacgag taccgctatg gcattcggca
     4141 gcgcctgtga tgcatcgacc agccaccatc cgaccacggt cagca
//
LOCUS       DATIRT010000065         4337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_302692, whole genome shotgun sequence.
ACCESSION   DATIRT010000065 DATIRT010000000
VERSION     DATIRT010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302692"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1498)
                     /locus_tag="VLN25_01625"
     CDS             complement(<1..1498)
                     /locus_tag="VLN25_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSQ09303.1"
                     /translation="MLVLPIALLAGGLTMTSATDVVIFAIACMALNILVGQTGLVSFG
                     HGAWFGIGAYAAALTQLHWFPGSMLWPTLFAVVLLLTSSIVAGFLVLRRRGVYFSLLT
                     LALSALVYSVAFRWTAFTGGENGLGGLTRATWLGADLESRWWYYGLVALCGLAVVFGL
                     SRFHRSPIGTVLNAIRENEQRAQFIGYATPRYKLVAFVLSSVLAGFAGVLFAFHHRFA
                     SADPTAVSFSGELLAMVIIGGMRSLLGPALGALFYILFREYLSIWTSHWLWFFGLLFV
                     GFIVFSPSGLVGVWQRLTAPLRRKSVEAAAMAGRAIEQGVQLPAFLKPRATDGGVVLA
                     AHGLAKSFGGIHALRDANVDVSAGSLHALIGPNGAGKTTLFNLISGLFPPDKGRVMLA
                     GVDVTGVPPYRIVAAGLARSFQITNLFPALDVQENLRLAVQSRHPGRFNGWTSTQAVD
                     PVMRETAELIRFLGLSGIERAEAGALSYGGQRLLDMGLALSSAPRVLLL"
     gene            complement(1547..2425)
                     /locus_tag="VLN25_01630"
     CDS             complement(1547..2425)
                     /locus_tag="VLN25_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSQ09304.1"
                     /translation="MLTQILEQVLNGLLAGAYYLLIALGLSLIFSLGGLVNLAHGAFY
                     ALGAYFAVIVAEHLGLGFAGSAIASPIVVAVIGLVIERFLFRRFYREDPALGLLLTFG
                     LAMVAEQSLRWVFGAAPVPFSIPTALRGQVFIGDFIYSKYRLLMLAVAVAAVAGLWVL
                     LQRTPFGRVVRAGVQNPDMVAALGISLKPYMASIVALSVGLAALAGVLLAPISGVHPA
                     MGAEILTAAFVVVVIGGLGSFWGVVWAALIVGVVRGLTVYFLPPAADASMYLLMLLVL
                     LLRPRGLTGERIQRFE"
     gene            complement(2491..3744)
                     /locus_tag="VLN25_01635"
     CDS             complement(2491..3744)
                     /locus_tag="VLN25_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSQ09305.1"
                     /translation="MNAPLSKSRRRLLHGAGAAAALGTIGFPAIVRSQSDAVRLGHLT
                     PLTGFLGPLGEYAVMGVNLAVEEINASGGINGRKVDLIAEDSVNPQTASTKAERLIER
                     DKVVAIVGEISSASGLAIAQVAQRNRILFFNTGCNSDELRGKSCNRYMFHTEAANSMY
                     VGAAGQALLRDGLVKGKKWYSVTADYAFGHDLLRVAKRFMQANGGTFAADELVATNVT
                     DFSPYLLKIRNAKPDLVVSNLAGAQITNFMKQYMEFGLPFPLAGFGFDTAVAWGAGKG
                     NVTGIWPLVWDSQVTSSTAQKFTAAFTKKYGKPPENQAWGDYLSTKHLAQALNELKST
                     DSTKVAEHLEKGAKFDVLKSREGYYRAWDHQLMQQMYTVTFKPESQVKDKWNLFNLSA
                     PVPADNQSLEIIAPTREENPCTMPA"
     gene            3896..>4337
                     /locus_tag="VLN25_01640"
     CDS             3896..>4337
                     /locus_tag="VLN25_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HSQ09306.1"
                     /translation="MPQRRTITTRVPIRDSAYAKLRELLIAGQLAPGDKVSPRELAAQ
                     LEVSPMPVREAVQRLAAEGALVVRPQSGARVPFMTRSRFIELTTIRIVLEGMAAARAA
                     KSASTAELTTIGQHLIRLQVSAARARPDPAVVIRGNQRLHFAIYR"
BASE COUNT          889 a         1423 c         1365 g          660 t
ORIGIN      
        1 cgagcaacag cacgcgcggc gcggaggaca gtgcaagccc catgtcgagc agccgctggc
       61 cgccgtacga cagggctccg gcttcggcgc gctcgatgcc tgatagcccc aggaagcgga
      121 tcagctccgc ggtctcgcgc atgaccggat ccacggcctg agtcgacgtc cacccgttga
      181 atcgtcctgg atgccgcgac tggaccgcga ggcgcagatt ttcctgcacg tcgagggccg
      241 gaaagagatt ggtgatctgg aatgagcgcg cgagtccggc cgccacgatg cggtaaggcg
      301 gcacgccggt cacatcgacc cccgccagca tgacgcggcc tttgtctggc ggaaacagcc
      361 cggaaatcag gttgaaaagc gtggtcttgc cggcgccatt gggaccgatc agcgcgtgca
      421 gcgaacccgc cgaaacgtca acgttcgcat cgcgcaaggc atggatgccg ccgaaggact
      481 tggccagccc gtgcgccgcg agcacaactc cgccgtcggt cgcacgtggc ttgaggaacg
      541 cgggcagttg cacgccctgc tcgattgcgc gacctgccat cgccgctgcc tcgaccgact
      601 tccggcgcag cggcgccgtc aaccgctgcc agacaccgac caggccactc ggtgagaaca
      661 cgatgaatcc cacgaacaac aggccaaaga accagagcca gtgggacgtc cagatcgaca
      721 ggtactcgcg gaacaggatg tagaaaagtg cgcccagtgc cggtccgagc aggctgcgca
      781 tgccgccaat gatcaccatc gccagcagtt caccggaaaa actgacagca gtcggatcgg
      841 cggaggcgaa gcgatggtga aaggcgaaca gcacgccggc gaatccggcc agaacagacg
      901 acagcacgaa cgcgaccagc ttgtagcgag gagtggcgta gccgatgaac tgagcccgct
      961 gctcgttctc gcggattgcg ttcagcacgg tgcctattgg cgaccggtgg aagcgggaaa
     1021 ggccaaagac cacggccaac ccgcacagcg caacgagacc gtagtaccac cagcggctct
     1081 cgagatctgc gccaagccag gtggcgcgcg tcaagccgcc cagaccgttt tcacctccgg
     1141 tgaacgccgt ccagcgaaac gcgaccgagt agacgagcgc cgacagcgcc agagtcagca
     1201 acgagaagta aacaccgcgt cggcgcaaga caaggaaacc ggccacgatc gacgaggtca
     1261 gcagcaagac cacggcaaac agcgttggcc acagcatcga gcctgggaac cagtgaagct
     1321 gcgtcagcgc tgccgcgtag gcaccgatgc cgaaccacgc gccgtggccg aacgacacga
     1381 ggccggtctg ccccaccagg atgttgagcg ccatgcaggc gatcgcgaag atcactacat
     1441 cggtcgcgct cgtcatcgtg aggccacccg ccagcagcgc aatgggcagc acaagcatcg
     1501 atagcgctgc gatgagaact ggtgaccgcc acacgctatg catcggctat tcgaagcgct
     1561 ggatgcgttc gccggtcaat ccgcgaggcc gcagcagcag gaccagtagc atcagcaggt
     1621 acatcgaggc atcggccgcc ggcggcagaa agtagaccgt caacccgcgc accacgccga
     1681 cgatcagcgc tgcccagacc accccccaga acgatccgag cccgccgatg acaacgacga
     1741 cgaatgcagc cgtcaggatc tcggcgccca tcgctggatg caccccggaa atcggagcga
     1801 gcagcacgcc ggccagcgcc gccagtccga cactgagcgc gacgatgctg gccatgtagg
     1861 gcttgagcga gatgccgagt gcggcgacca tgtcggggtt ctgcacgccg gcgcgaacca
     1921 cacgtccgaa aggtgttcgc tgcaacagga cccagagccc cgccacggcc gcaacggcta
     1981 cggcgagcat caacagccgg tacttcgagt agatgaagtc gccaatgaat acctgtccgc
     2041 gcagcgcggt gggaatcgag aacggcaccg gcgccgcgcc gaacacccag cgcagcgact
     2101 gctcggcgac cattgccagg ccgaaggtca gcagcaagcc gagggccgga tcctcgcgat
     2161 aaaaccgccg aaagaggaat cgctcgatca ccaggccgat cacggcgacg acgatcggcg
     2221 acgcgatcgc cgatcctgcg aaaccgaggc cgaggtgctc ggcaacgatg acagcgaagt
     2281 acgcgccgag cgcatagaac gcgccatgcg ccagattgac cagcccgccc agcgaaaaga
     2341 tcagcgacag gccgagcgcg atcagcaggt agtaggcacc cgcgagcagg ccattgagaa
     2401 cctgctcgag gatctgcgtc agcatggatt cgaagcgcgg cgcggaccgg tggctacgga
     2461 atgcgccgcg aattcacgtc tatccggcca tcaggcgggc atcgtgcagg gattttcctc
     2521 gcgcgtcggc gcgattattt ccaggctctg gttgtcggcc ggcaccggag cggacaggtt
     2581 gaacaggttc cacttgtcct tgacctgcga ctccggcttg aaggtcaccg tgtacatctg
     2641 ttgcatgagt tggtggtccc aggcgcggta atagccctcg cgcgacttga gcacatcgaa
     2701 cttggcgccc ttttccagat gctccgccac cttggtcgaa tccgtggact tcagttcgtt
     2761 cagtgcctgc gccaggtgct tggtcgagag gtagtcgccc catgcctggt tctccggtgg
     2821 cttgccgtac ttcttggtga aggccgcggt gaacttctgc gcggtcgacg aagtcacctg
     2881 ggagtcccac accaggggcc agatgccggt gacattgccc ttgcccgcgc cccatgcgac
     2941 ggccgtatcg aatccaaatc cggccagcgg gaacggcaag ccgaactcca tgtactgctt
     3001 catgaagttg gtgatctgcg ccccggccag attggaaacg acgagatccg gcttggcatt
     3061 gcggatcttc aggagatacg gcgaaaaatc cgtgacgttg gtggcgacga gttcgtccgc
     3121 ggcgaatgtg ccgccattgg cctgcatgaa gcgtttggca acgcgcagca gatcgtgccc
     3181 gaaggcgtaa tccgccgtca cgctgtacca cttcttgccc ttgaccagac cgtcgcgcag
     3241 cagcgcctgc ccggccgcgc cgacatacat gctgttagcg gcttcggtat ggaacatata
     3301 gcggttgcac gacttgccac gcagttcatc cgaattgcag ccggtgttga agaacaggat
     3361 cctgttgcgc tgggcgacct gcgcgatcgc caggcccgat gccgaactga tctcgccgac
     3421 gatcgcgacc accttgtcac gctcgatcag gcgttccgcc ttggtcgatg cggtctgcgg
     3481 attgacgctg tcttcggcaa tcagatcgac cttgcgccca ttgatcccgc cggacgcatt
     3541 gatttcttca accgccaggt tcacgcccat cacggcatac tcgccgagtg gaccgagaaa
     3601 accggtcagc ggtgtcagat ggcccagtcg tacggcatcg gactggctac ggacgatggc
     3661 cggaaatccg atagtcccga gtgccgccgc ggcaccagcg ccatgtaaca gccggcgacg
     3721 cgacttcgac agcggtgcat tcatgttgtc tcctcgttgt ttaagtgccg gagtgcaacc
     3781 aaaggcagca caccgcggga ttgatcgccg ccgcacgctg cgattccgcc gccgtgcaac
     3841 tgcgatcgca gttatagtcg agccgactgt cactgtccag cgaacgtccc cattcatgcc
     3901 gcagcgccgc acgatcacga cgcgggtccc gatccgggac agcgcctacg ccaagttgcg
     3961 cgaattgctg atcgccgggc aactcgcgcc gggtgacaag gtgtcgccgc gcgaactggc
     4021 agcgcagctc gaggttagcc cgatgcccgt gcgcgaggcg gtccagcggc tggccgctga
     4081 gggcgcgctt gtggtgcgcc ctcagagcgg cgcgcgggtg ccgttcatga cccgcagccg
     4141 gttcatcgag ttgacgacaa tccgcatcgt gctcgaaggc atggccgccg cccgtgcggc
     4201 gaagtcggct tccacggccg agctgaccac catcgggcag cacctgatca ggctgcaggt
     4261 ctcggccgca cgcgcccgac ccgatccggc cgtcgtgatc cgcggcaacc agcggctgca
     4321 cttcgccatc taccgca
//
LOCUS       DATIRT010000066         4276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_204372, whole genome shotgun sequence.
ACCESSION   DATIRT010000066 DATIRT010000000
VERSION     DATIRT010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204372"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1308
                     /gene="ppk1"
                     /locus_tag="VLN25_01645"
     CDS             <1..1308
                     /gene="ppk1"
                     /locus_tag="VLN25_01645"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HSQ09307.1"
                     /translation="HFGNAMRLEVLQTCPAPLQQRLQSQFQLPDEAVFRVDGPVNLVR
                     LNQLVDHLTQPPLRWAPFVPAWPTKLATGSDMFEAIRRSDILMHHPFESFEPIIEFLR
                     QAAVDAKVVAIRMTLYRTGISSVPVDILEEAARRGKEVTVIVELKARFDEEANINLAE
                     RLEAVGAQVVYGVVGLKTHGKMTLVMRREEVRGKQRLVPYAHLGTGNYHTTTTKLYTD
                     FGMLTANPEICEDVEKVFMHLTSLTKVERLKHLWMAPFTLHRSMLVALAAEVEFAKSG
                     KPARVIAKMNALTDEATILALYAASQAGVKVDLIVRGACALRPGVKGLSENIRVRSIV
                     GRFLEHHRIYYFRNGGKPRVYLSSADWMGRNLFRRIEVAWPVLDPKLARRVIDEGLKP
                     YLEDTRDAWVLTGDGNYRPPRRGANAVSSQHELLARLAAGTGV"
     gene            1312..1776
                     /gene="sixA"
                     /locus_tag="VLN25_01650"
     CDS             1312..1776
                     /gene="sixA"
                     /locus_tag="VLN25_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HSQ09308.1"
                     /translation="MELILWRHAEADIGEPDLGRKLTARGEKQAHRIAEWLHAQLPDS
                     ARVLVSPATRAQQTARALSDVSHRKFRTVDEIAPGASVRDVLDAADWPRAKAPVVIVG
                     HQPTLGHVASFLLAGEVQDWSVKKSGVWWLSSRERDGEEQTVLRAVIGPDLA"
     gene            complement(1834..3294)
                     /locus_tag="VLN25_01655"
     CDS             complement(1834..3294)
                     /locus_tag="VLN25_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016475230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HSQ09309.1"
                     /translation="MNLAAVDLGSNSFRLEIGRVEGTHIEREGYWKETVRLAAGLDDT
                     NKLSKKSIETAIETLARMNERLRGMPLEQVRAVGTQTLRQARNINDFLVEAQSALGYP
                     IEVISGREEARLVFEGCMHTLPPSNLRRLVVDIGGASTEIIVGRGFSATNAESFKVGC
                     VNTSLRFFKDGRIDRPALKKAQVAAAAEFEEAIAAFSRAQWEEAFGSSGTVGAVADIM
                     RAQGWSDGTINSEGLLKLRHAMLDAGEIRRIRLAGLKPDRQEVLAGGVAVLSAVFDTF
                     GIDEMRPARGALRVGVLYDLLGRREQNDLRDATVERMLDRFGVDREQAARVAATARQL
                     YAELAPAAPDETVKRLAWAALLHEVGFAISHSDYHKHSAYLVQHADLAGFSTSDQEHI
                     ALLVLGQRGNLRKVHVALEDRDRSAKILVLRLAVIMNHARRSVELPRWTLKFGRGIEL
                     GIDAEWLSRHPLTQFLLEEEAMHWSRVGITFVVRTP"
     gene            complement(3291..4241)
                     /gene="ftsY"
                     /locus_tag="VLN25_01660"
     CDS             complement(3291..4241)
                     /gene="ftsY"
                     /locus_tag="VLN25_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016475229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HSQ09310.1"
                     /translation="MISPGSSEGAKGGWLARLKRGLSKTRVNIVGLFSGGVVDDAFLE
                     ELEMALIGADVGVSTSAQLVEAVRSEIKLRGLKSQDEVRIALRDELARRLAPAEGRFD
                     LTHDRPYVVMVCGVNGSGKTTSIGKLAKWFAGQQRSVLLAAGDTFRAAAREQLAIWGA
                     RNNVDVIAQSGGDPAAVVFDAVSAAKARGVDVLIADTAGRLPTQAHLMEELKRIRRAQ
                     DKSLPGAPHEVILIVDGTNGQNALAQVQAFDAAVGLTGLVVTKLDGTAKGGVLAAIAA
                     ARGDRPLPILFIGVGEGIDDLQPFSAVEFADALVGPGAAQ"
BASE COUNT          720 a         1419 c         1400 g          737 t
ORIGIN      
        1 cacttcggca atgccatgcg gctcgaagtt ctgcagacct gccccgcgcc gctgcagcag
       61 cggctgcaga gtcagttcca gttgcccgac gaggcggttt ttcgcgtcga cggcccggtc
      121 aacctggtgc ggctgaacca gctggtcgat cacctgaccc aacccccgct gcggtgggcg
      181 ccgttcgtgc cggcttggcc gacgaaactg gcgaccggct ccgacatgtt cgaggcgatc
      241 cggcgcagcg atatcctgat gcaccacccg ttcgagagtt tcgagccgat catcgaattc
      301 ctgcgccagg cggcggtcga tgccaaggtc gtggcgatcc gaatgactct gtatcgcacc
      361 ggcatcagct cggtgccggt cgacatcctc gaggaagcgg cacgtcgcgg caaggaagtc
      421 acggtgatcg tcgagctgaa ggcgcgcttc gacgaggagg ccaacatcaa tcttgcggag
      481 cgtctcgagg ctgtcggcgc acaggtcgtt tacggggtgg tcgggctgaa aacccacggc
      541 aagatgacgc tggtgatgcg ccgcgaggag gtgcgcggca agcagcgact tgttccctac
      601 gcacacctgg gcactggcaa ctatcacacg accacgacca agttgtacac cgacttcggg
      661 atgctgacgg caaaccccga gatctgcgaa gacgtcgaaa aggtgttcat gcacctcacc
      721 agcctgacca aggtcgaacg tctgaagcat ctgtggatgg cgccgttcac tctgcatcgg
      781 agcatgctgg tggcgctcgc ggcggaggtg gaatttgcga aatcaggcaa gccggcgcgc
      841 gtcattgcga agatgaacgc gctgaccgac gaggcgacga tcctggcact gtatgcggcc
      901 tcgcaggccg gagtgaaggt cgacctcatc gttcgcggcg cctgcgcgct gcgccccggc
      961 gtcaagggcc tgtcggagaa catccgggtg cgctcgatcg tcgggcgctt cctcgagcac
     1021 catcgcattt attacttccg caacggcggc aagccgcgcg tgtacctgtc gagcgctgac
     1081 tggatggggc gcaacctgtt ccgccgcatc gaggtggcct ggccggtgct ggatcccaag
     1141 cttgcacggc gcgtgatcga cgaggggttg aagccctatc tcgaggacac gcgcgatgcg
     1201 tgggtgctga cgggggacgg caactatcgc ccgccccgcc ggggcgcgaa tgcggtttcg
     1261 tcgcagcatg agctgctggc cagactcgcc gcaggtactg gggtctgacg cttggagctc
     1321 atcctctggc gccacgcaga agccgacatc ggcgagcccg acctcggccg caagttgacg
     1381 gcgcgcggcg agaagcaggc gcaccgtatt gccgagtggc tgcatgcgca gctaccggac
     1441 agcgctaggg tcctggtcag tccggccaca cgggcgcagc agacagcgcg cgcattgtcg
     1501 gatgtctcgc atcgcaagtt ccgtaccgtt gacgagatcg cccccggcgc cagcgtgcgc
     1561 gacgtgctcg acgctgccga ctggccccgc gccaaggccc ccgttgtgat cgtcggccac
     1621 cagccgacgc tcggccacgt cgccagcttc ctgctggcag gcgaggtgca ggactggtcg
     1681 gtaaagaagt ccggagtgtg gtggttgagc tcgcgcgagc gggacggcga agaacagacc
     1741 gtcctgcgtg cggtgatcgg ccccgacctg gcctgacgtg gttgctggag catcccggaa
     1801 cgccccggat cgtcctgatg tgtcctgggc gacctaaggc gtgcgcacga cgaaggtgat
     1861 gccgacgcgg gaccaatgca tcgcttcctc ctccagcagg aactgggtca gcggatggcg
     1921 cgagagccac tcggcgtcaa tgcccagctc gatgccgcgc ccgaacttca gtgtccatcg
     1981 cggcagttcc acgctgcgac gcgcgtgatt catgatgacc gcaagccgga gcacgagaat
     2041 cttcgcagaa cggtcacggt cctcgagtgc gacgtggacc ttgcgcaggt tgccccgctg
     2101 gccgagcacc agcagtgcga tgtgttcctg gtccgatgtc gagaaccccg ccaggtccgc
     2161 atgttgcacg agataggccg agtgcttgtg atagtcgctg tgcgaaatcg caaacccgac
     2221 ttcgtgcagc aatgcggccc acgcaagtcg tttgactgtt tcgtccgggg ccgccggagc
     2281 cagttcggca tacagttgcc gggcagtcgc cgcgacgcgt gcggcttgct cccggtcgac
     2341 gccgaagcgg tcgagcatgc gctcgaccgt ggcatcgcgc aggtcattct gctcgcgcct
     2401 tccgagcagg tcgtacagca cgccgacgcg cagtgcgcct cgagccggcc gcatctcgtc
     2461 gatgccgaac gtgtcgaaca ccgcggaaag cacggcgaca ccgccggcga ggacctcctg
     2521 gcggtcgggc ttcagtccgg ccaggcggat gcggcggatc tcgccagcat cgagcatcgc
     2581 atggcgcagc ttcagcaggc cctcgctgtt gatcgtaccg tctgaccacc cttgcgctcg
     2641 catgatgtcc gcgacggcgc ccaccgtccc gctggaaccg aaagcttcct cccactgtgc
     2701 gcgcgagaac gctgcaatcg cttcctcgaa ttcggcagcg gctgccacct gggccttctt
     2761 cagcgccggc cggtcgattc gaccgtcctt gaagaagcgc agtgacgtgt tcacgcagcc
     2821 gaccttgaac gactcggcgt tggtcgcgct gaagccccgg ccgacgatga tctccgtcga
     2881 ggcaccgccg atgtcgacaa cgagccgccg cagattggac ggcggcaggg tgtgcatgca
     2941 gccctcgaac accagccgcg cttcttcgcg acccgagatc acttcgatcg gatagccaag
     3001 cgcgctctgc gcttccacga gaaaatcgtt gatgttgcgg gcctggcgca aggtctgggt
     3061 gccgaccgcg cgcacctgct cgagcggcat gccacgcagg cgttcgttca tgcgggcgag
     3121 cgtctcgatc gcggtctcga tggacttctt gctgagcttg ttcgtgtcgt cgagtccggc
     3181 tgccagccgc acggtttcct tccagtagcc ctcgcgctcg atgtgcgtgc cttcgacgcg
     3241 gccgatctcg agccggaaac tgttcgaacc gaggtcgacg gcggcgagat tcactgcgcg
     3301 gctccaggcc ccaccagcgc gtcggcgaac tcgacggccg aaaacggttg tagatcgtcg
     3361 atcccttcac cgacgccgat gaaaaggatc ggcaggggcc ggtcgccgcg cgccgcggcg
     3421 atcgctgcca atacgccccc cttggcggtg ccatcgagct tggtgacgac aagtcccgtc
     3481 aggccgaccg ccgcgtcgaa cgcctgcacc tgcgccagcg cgttctggcc gttggtgcca
     3541 tcgacgatga ggatgacctc gtgcggcgcg ccgggcagcg acttgtcctg tgcgcgccgg
     3601 atgcgcttga gttcctccat caggtgcgcc tgagtcggca aacggcccgc cgtgtcggcg
     3661 atcaggacat cgaccccgcg cgccttggcc gccgagactg catcgaagac gacggccgcc
     3721 ggatcgccgc cgctttgcgc gatgacgtcg acgttgttgc gcgcccccca gatggcgagt
     3781 tgctcgcgtg ccgccgcgcg gaaagtgtct ccggccgcga gcagcaccga gcgctgctgt
     3841 ccggcgaacc acttggccag cttgccgatc gaagtcgtct tgccggaacc gttcacgccg
     3901 cacaccatga cgacgtaggg ccgatcgtgt gtcaggtcga atcgtccttc cgccggtgca
     3961 aggcggcgtg ccagctcatc gcgaagcgcg atccgcacct catcctgtga cttcaatccc
     4021 ctgagcttga tctccgagcg cacggcctcg accaactgtg ccgatgtcga cacgccgacg
     4081 tccgctccga tcagcgccat ttcgagttct tcgaggaatg cgtcatcgac cacgccaccc
     4141 gagaacaggc cgacgatgtt gacgcgcgtc ttcgacaggc cgcgcttgag tcgcgcgagc
     4201 cagccgccct tggcgccttc agaactaccg gggcttatca ttgaacgatg cggggaaagc
     4261 gatcggggcg tgctgc
//
LOCUS       DATIRT010000067         4600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_499264, whole genome shotgun sequence.
ACCESSION   DATIRT010000067 DATIRT010000000
VERSION     DATIRT010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499264"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..374)
                     /locus_tag="VLN25_01665"
     CDS             complement(9..374)
                     /locus_tag="VLN25_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09311.1"
                     /translation="MRTTPSRFSAFTGNVEPPFEASPTAGPAFGRVAESAIEQRYPAV
                     TRSLTLLWGHPEMNQYFEKVSSGQDATLNLDPQAMSELMLLAAVHQRVCPYVPAKKVE
                     DFYGAGRWADTWKPARLRG"
     gene            complement(590..1075)
                     /locus_tag="VLN25_01670"
     CDS             complement(590..1075)
                     /locus_tag="VLN25_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HSQ09312.1"
                     /translation="MPSFDAVCKHDMVEVRNATDQANKEITNRFDFKGSDARVEMKEF
                     ELTAFADDDFKLGQVRDVLTSKMAKRGVDVRLLDLGKPEKMSGDKLKQVIKVRNGIES
                     TLARKMVALVKESKLKVSASIQGDTVRISGAKRDTLQEVIALLRSQVTEVPLTFDNFR
                     D"
     gene            1136..2155
                     /gene="murB"
                     /locus_tag="VLN25_01675"
     CDS             1136..2155
                     /gene="murB"
                     /locus_tag="VLN25_01675"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HSQ09313.1"
                     /translation="MPVRIESDVPLQRYNTFGVEARAAHFARVASIEELVGLVEDPQW
                     GAMPRLVLGGGSNVLFTGDFDGLVIKVEIMGIEVLETKGGQHRVRIGAGENWHATVER
                     LLERGLPGLENLALIPGNVGAAPIQNIGAYGVELAERFESLGAYDASSHARVTLDDEQ
                     CRFGYRDSVFKQEPGRYVVLDVTLALPADWRPVAGYADVENELKARQCVNPQPRDVFD
                     AVVAIRRRKLPDPALIGNAGSFFKNPIVPRATRDQMREKFPSLVSYDIGGGRCKLAAG
                     WLIDACGLKGHARGQAVVYEKQALVLVNRGGASGRDVLELAREVQERVRQKFGVALEP
                     EPAIV"
     gene            complement(2462..3802)
                     /gene="argG"
                     /locus_tag="VLN25_01680"
     CDS             complement(2462..3802)
                     /gene="argG"
                     /locus_tag="VLN25_01680"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HSQ09314.1"
                     /translation="MSTILQHLPVGQKVGIAFSGGLDTSAALLWMRKKGAIPCAYTAH
                     LGQPDEPDYDEIPRRALEYGAEKARLVDCRAQLVSEGIAALQCGAFHISTAGLTYFNT
                     TPIGRAVTGTLLVTAMKEDDVHIWGDGSTYKGNDIERFYRYGLLANPSLKIYKPWLDQ
                     DFIDELGGRAEMSEIMKQAGFAYKMSTEKAYSTDSNMLGATHEAKDLEYLASNVRIVQ
                     PIMGVAFWRDDVPIKREEVTVRLDEGQPVALNGIEFTDPVELMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGLALLFIAYERLLTGIHNEDTIEQYRDNGRRLGRLLYQG
                     RWFDPQAIMLRETAQRWVASAITGEVSIELRRGNDYSILNTESPNLTYKPERLTMEKG
                     ESSFSPQDRIGQLTMRNLDITDTRDKLMTYAKAGLLSGPSGTTLPGLTNEVKKK"
     gene            complement(3851..>4600)
                     /gene="argF"
                     /locus_tag="VLN25_01685"
     CDS             complement(3851..>4600)
                     /gene="argF"
                     /locus_tag="VLN25_01685"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HSQ09315.1"
                     /translation="TRVSFEAGMYQLGGSVIHITTGDSQLGRAEPIEDTARVISRMVD
                     LVMIRTYEQSKIERFAEFSRVPVINGLTNEYHPCQILADIYTWLEHRGPITGRTVAWI
                     GDANNMAYTWIEAAQLLGFRVNVSTPPGYALDSRLAPAGDHIAVHADPMDAARGADLV
                     TTDVWTSMGYENENEARRVAFSDWCVDEEMMRAAHPDALFMHCLPAHRGEEVTAAVID
                     GPQSVVWDEAENRLHAQKALMEYLLLGREVD"
BASE COUNT          770 a         1419 c         1535 g          876 t
ORIGIN      
        1 ggcgtgtgct agccgcgcag gcgcgcgggt ttccaggtgt cggcccagcg accggcccca
       61 tagaagtctt cgactttctt ggcaggcacg tacgggcaca cccgctgatg tacggcggca
      121 agcaacatca gctcggacat ggcctgcgga tcgagattca gtgtcgcgtc ctggccgctc
      181 gagacctttt cgaagtactg gttcatctcc gggtgacccc agagcagggt cagtgatcgg
      241 gtgaccgccg ggtagcgctg ctcgatcgcc gactcggcca cgcggccgaa tgccgggccg
      301 gccgtgggac tcgcctcgaa gggcggctcg acgttgccgg tgaaggcgct gaaacgagag
      361 ggggtcgttc gcataccgta ttcaggttaa tccagttcaa tccggtgaag caaggccgcg
      421 cgtcggatcc gagggtgtgt cgatcgaact gcggccgcag tcaaaccgta agcgtggagc
      481 ccgccggatt caatcgtgag ccgagtgagc ggcgcgcacc cggcgccggg cggggcagga
      541 ctgtcgcccg cgcgggagca gcggtcgccg gcgaccgctg gccggcgcct cagtcgcgga
      601 agttgtcgaa ggtcagcgga acctcggtga cctgcgaacg cagcagggcg atgacctcct
      661 gcaaggtgtc ccgcttggca ccgctgatgc gcaccgtatc gccttggatg cttgcgctga
      721 ccttgagctt gctctccttg accagcgcga ccatcttcct ggcgagcgtc gattcgatgc
      781 cgttacggac cttgatcacc tgcttcaact tgtcgccgga catcttttcg ggtttgccga
      841 ggtcgagcag gcgcacgtcg acgccgcgct tggccatctt cgatgtcagc acgtcgcgca
      901 cctggccgag cttgaagtcg tcatcggcga acgcggtcag ttcgaactcc ttcatctcga
      961 cacgcgcgtc cgagcccttg aagtcgaacc gattggtgat ctccttgttg gcctggtcgg
     1021 tcgcattgcg cacttcgacc atatcgtgtt tgcagactgc atcgaatgac ggcatgaacc
     1081 ttctccggaa ctggatcgca acgggagtct atgcgcacgc ccctacaatc ctccgatgcc
     1141 cgtgcgcatt gaatcagacg tcccacttca gcgctacaac acctttgggg ttgaggcgcg
     1201 tgctgcccac tttgcgcggg tcgcgtccat cgaggaactc gtcggcctgg tcgaggatcc
     1261 gcagtggggt gcgatgccgc ggctggttct cgggggcggc agcaacgtgc tcttcaccgg
     1321 tgacttcgac ggcctggtga tcaaggtcga gatcatgggc atcgaggtgc tcgaaacgaa
     1381 gggcgggcag catcgggtgc gcatcggcgc cggcgagaac tggcacgcca cggtcgagcg
     1441 gttgctggag cggggcttac cgggcctgga gaacttggcg ctgatcccgg gcaatgtcgg
     1501 cgcggcaccc atccagaaca ttggagccta cggtgtcgag ctggccgaac gattcgagtc
     1561 actcggcgcg tacgacgctt caagccacgc cagggtcacg cttgacgacg agcagtgccg
     1621 cttcgggtat cgcgacagcg tcttcaagca ggagcccgga cgctatgtcg tgctcgatgt
     1681 cacgctggca ttgccggccg actggcgacc ggttgccggc tatgccgacg tggaaaacga
     1741 gttgaaggcg cgtcagtgcg tcaacccgca gccgcgcgat gtcttcgatg ccgtcgttgc
     1801 gatcaggcgc cgcaagttgc cggacccggc gctcattggc aacgccggga gcttcttcaa
     1861 gaacccgatc gtgccgcgcg cgacccgcga tcagatgcgg gagaagtttc cgtcgctggt
     1921 gagctacgac atcggcggtg gccgctgcaa gctcgccgcc gggtggctca tcgatgcctg
     1981 tggactgaag ggccatgccc gaggccaggc ggtggtgtac gagaagcagg cgctggtcct
     2041 cgtgaatcgc ggcggggcgt cgggtcggga cgtgctcgaa ctggcgcgcg aggttcagga
     2101 gcgggttcgg caaaaattcg gagtcgcact ggaacccgag ccggccatcg tctgagcgag
     2161 tctttgcgag acaaaaaaag gcccgccgaa gcgggccaga atttcgctgc aacctaggca
     2221 gtcgtcgcag ccgccggtcg gagagctcgc ttgcggtgag gagaacgcgc agagcaccgg
     2281 cggccacgaa cgcttttact gtggcaccaa aagcgtcgcc gtgcattcat ccaaaagtct
     2341 tgtgacgctc atgccgcgat cggagccgac atcgcgtcac cgcatttgct tgcctgtgcc
     2401 cgcaggcgcg gagccgttgt ccgcacccgc tccgggcgag acgatctgga agatcgaccg
     2461 actacttctt cttgacctcg tttgtcaggc ccggcagcgt cgtgcccgac gggcccgaca
     2521 gcaggccggc cttggcatag gtcatcagct tgtcgcgcgt gtcggtaatg tccaggttgc
     2581 gcatcgtcag ttgcccgatc cgatcctgcg gcgagaagct cgactcgcct ttttccatcg
     2641 tcaatcgctc gggcttgtaa gtcaggttgg gcgactcggt attgaggatt gagtagtcgt
     2701 tgccacgccg cagctcgatc gacacctcgc cggtgatcgc acttgcgacc caacgctgcg
     2761 cggtctcgcg cagcatgatc gcctggggat cgaaccagcg tccctggtag agcagccgtc
     2821 cgagtcgccg gccgttgtcg cgatactgct cgatcgtgtc ctcgttatgg atgcccgtca
     2881 gcagccgctc gtaggcgatg aagagcagcg ccagtccggg cgcctcgtag atgccgcggc
     2941 tcttggcctc gatgatgcgg ttctcgatct ggtccgacat gcccaggccg tggcggccgc
     3001 cgatgcggtt ggcttcgagc atcagctcga ccggatcagt gaactcgatg ccgttgaggg
     3061 cgaccggctg gccctcgtcg aggcgcactg tgacttcctc gcgcttgatg ggcacgtcat
     3121 cgcgccagaa ggcgacgccc atgatcggct gcacgatgcg cacgttgctg gccaggtact
     3181 cgagatcctt ggcctcgtgc gtggcaccga gcatgttcga gtcggtcgaa tacgctttct
     3241 cggttgacat cttgtacgca aagccggcct gcttcatgat ctccgacatt tcagcgcgcc
     3301 cgccaagttc gtcgatgaag tcctggtcga gccagggctt gtagatcttc agtgacggat
     3361 tggcgagcag gccgtagcgg tagaagcgct cgatgtcgtt gcccttgtag gtgctgccgt
     3421 caccccagat gtggacgtca tcttccttca tcgcggtgac cagcagcgtg ccggtcactg
     3481 cacggccaat cggagtcgta ttgaagtacg tgaggccggc ggtcgagatg tgaaaggcgc
     3541 cgcattggag cgcggcgatg ccctcggaaa ccaactgcgc gcggcagtcg accagtcgag
     3601 ccttttcggc accgtactcg agcgcgcggc gcgggatctc gtcgtagtcg ggttcgtcgg
     3661 gctgcccgag atgggctgta tacgcgcagg ggattgcgcc cttcttgcgc atccacaaca
     3721 gcgccgcgct ggtgtcgagt ccgccggaaa aggcgatgcc gaccttctgg ccgacgggca
     3781 ggtgctgcag gatcgttgac atggtgggtc gctggcgaaa gcgcgccgtc gacgcgcgtg
     3841 agtcggtgaa ctagtcgacc tcgcggccga gcagcagata ttccatcagc gccttctgcg
     3901 catggagcct gttttcagcc tcgtcccaga cgacggactg ggggccgtcg atcaccgcag
     3961 cggtgacttc ctcgccgcga tgcgccggca ggcaatgcat gaagagtgca tccgggtgtg
     4021 ccgcgcgcat catttcctcg tccacgcacc agtcggagaa cgcgacgcgg cgcgcctcgt
     4081 tttcgttctc gtagcccatg ctggtccaga cgtcggtcgt caccaggtcg gcgccgcggg
     4141 ctgcgtccat cgggtcggca tggaccgcaa tgtggtcgcc ggccggagca agccgtgaat
     4201 cgagcgcata ccccggtggc gtggagacgt tgacacgaaa gccgagcagc tgggccgcct
     4261 cgatccatgt gtaggccatg ttgttggcat cgccgatcca cgccacggtg cggcccgtga
     4321 tggggccgcg atgctcgagc cacgtataga tgtcggccag gatctggcaa gggtggtact
     4381 cgttcgtcag gccgttgatc accggcacgc gcgaaaattc cgcgaagcgt tcgatcttgg
     4441 actgttcgta ggtgcggatc atcacaaggt cgaccatgcg cgagatcacg cgcgcggtgt
     4501 cctcgatcgg ctcggcgcga ccgagctgcg aatcgccggt ggtgatgtgg atcaccgagc
     4561 cgcccaactg gtacatgccg gcctcgaacg aaacgcgcgt
//
LOCUS       DATIRT010000068         3149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_696198, whole genome shotgun sequence.
ACCESSION   DATIRT010000068 DATIRT010000000
VERSION     DATIRT010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696198"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /gene="porA"
                     /locus_tag="VLN25_01690"
     CDS             <1..1082
                     /gene="porA"
                     /locus_tag="VLN25_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HSQ09316.1"
                     /translation="FAALSVAIGASATGARTYTATSSQGLLFMAEAVYNAAGLGLPIV
                     MTIGNRAIGAPINIWNDHSDSMSLRDSGWIQLHAETNQEALDLHIQAFRLAETLSVPV
                     MVCMDGFILTHAVERVDMPTQVEVDGYLLPFEPRQVLDPREPVSIGAMVGPEAFMEVR
                     YLAHRKQLSALELIPQLAEEFELRFGRSSGGLLRTYRADDAETIVVALGSVNGTIQEV
                     VDAMRDDGVRVGSVAITAFRPFPLDALRAAIGHAKRVVVLEKSLALGLGGILSDGVRK
                     ALSGIQLKGYTVIAGLGGRNITRASLRKLFESAVREELETVTFLDLNTSVVERELVRE
                     RLQRRSGPTAESVLRQLGTVASGIG"
     gene            1100..2104
                     /locus_tag="VLN25_01695"
     CDS             1100..2104
                     /locus_tag="VLN25_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HSQ09317.1"
                     /translation="MRQPVKFYQTGTFTVGNRLLAEDERSVQANMQRTNSLNSGHRAC
                     QGCGEALGARYALDAAMRATDNQLIAVNATGCLEVFSTPYPETSWQIPWIHSLFGNAA
                     AVASGVASALRVQGKEHVRVIAQGGDGGTTDIGFGCLSGMFERNDDVLYICYDNEAYM
                     NTGVQRSSATPLAARTATTPAVGPAPGNVSGTGKNVPLIAMAHLVPYVATASVANLRD
                     LEDKVTRAMSIRGARYVHIHVPCPLGWGSVPADTIKVARAAVESGLFPLFEAEHGEVT
                     GRTPIRHRVPVDDYLKLQKRFAHLFSPKRDEARLAQIQAIADRNISTYGLLDLEGARG
                     "
     gene            2097..>3149
                     /locus_tag="VLN25_01700"
     CDS             2097..>3149
                     /locus_tag="VLN25_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSQ09318.1"
                     /translation="MDKPFAITLDPGSSLANLTGSWRTSRPVYVDRLPPCNNACPAGE
                     NIQQWLYHAEGGDYQAAWRQLVQDNPLPSIMGRVCYHPCETACNRAHIDEAVGINSVE
                     RFLGDEALKRHWKFDPPTKETGKRVLVVGAGPSGLSAAYHLRRRGHAVTIYEAGPLAG
                     GMMRFGIPTYRLPRDVLDAEVRRILDLGVELKLNRKVSNILDEMREGGFDAAFLAVGA
                     HIAKRAYIPAGEAAHMLDAVSVLRSMEGEDKPMLGRRVVVYGGGNTALDVARTAKRMG
                     AEPIIVYRRTREKMPAHDFEVEEALAEGVLIKWLSTIRNVDDSGTMTVEKMALDEKGF
                     PQPTGEFETLEADSLVL"
BASE COUNT          533 a         1031 c         1050 g          535 t
ORIGIN      
        1 agttcgcggc gctgtcggtc gcgatcggcg cctcggcgac gggcgcccgg acctacaccg
       61 cgaccagcag ccaggggctg ctcttcatgg ccgaggcggt ctacaacgcc gctggcctgg
      121 gcctgccaat cgtgatgacg atcggcaatc gcgcgatcgg cgcacccatc aatatatgga
      181 atgaccactc cgacagcatg tccttgcgtg actcggggtg gatccagctg cacgcggaaa
      241 cgaaccagga agcgctagac ctgcacattc aggcgttccg gctggccgaa acactgtcgg
      301 tcccggtcat ggtctgcatg gacggcttca tcctgacgca cgccgttgaa cgcgtcgaca
      361 tgcccacgca ggtcgaggtc gacggctacc ttctgccgtt cgagccgcgt caggtgcttg
      421 atccgaggga acccgtcagc atcggggcaa tggtcggccc cgaggcgttc atggaggttc
      481 gatatctcgc ccaccgcaag cagctgagcg cgctcgaact gatcccgcaa ctggccgagg
      541 agttcgaact gcgcttcggg cggtcgtccg ggggactgct gcggacttac cgggccgatg
      601 acgccgagac catcgtcgtg gcgctgggct cagtgaatgg cacgatccag gaagtggtcg
      661 atgcgatgcg tgacgacggg gtgcgggttg gctccgttgc gatcacggca ttccggcctt
      721 tcccgctgga cgcgctgcgt gctgccatcg gccatgcgaa gcgcgtcgtc gtgctggaga
      781 agagcctcgc gctgggtctc ggcggcatcc tgtccgatgg cgtgcgcaag gcactgtccg
      841 gcatacagct caagggctat acggtgatcg ctggcctggg ggggcgcaac atcacacggg
      901 cgtcgctgcg caagctcttc gaatccgccg tgcgcgagga actcgagacg gtcaccttcc
      961 tcgatctgaa cacttcagtc gtcgagcgcg aactcgtccg tgaacggctg caacgccgca
     1021 gcggccccac tgccgagtcc gttctgcggc agctgggcac cgtggcctcg ggcatcggct
     1081 gaaagggtag aacatggacc tgcgccagcc tgtcaagttc taccagaccg ggactttcac
     1141 ggtcggcaat cgcctgctcg ccgaggacga gcgcagcgtg caggcgaaca tgcagcggac
     1201 caattcgctg aactcgggtc atcgtgcctg ccagggctgc ggtgaggcgc tcggtgcgcg
     1261 ttacgcgctc gatgcggcga tgcgcgcgac cgacaatcag ctgattgcag tcaatgcgac
     1321 cggttgcctc gaggtgttct ccacgccgta tccggagacc tcatggcaga ttccctggat
     1381 tcactcgctg ttcggcaatg cggccgcagt ggcgtcgggc gtcgcgtcgg cgctccgggt
     1441 gcagggcaag gagcacgttc gggtgatcgc ccagggcggc gacggcggca cgaccgatat
     1501 cggtttcggc tgcctgtcag gaatgttcga gcgcaacgac gacgtgctct acatctgcta
     1561 cgacaacgag gcgtacatga ataccggcgt gcagcgttcg agcgccacgc cgctcgcagc
     1621 gcgcaccgcc accacgccgg ccgtcggccc ggctccaggc aatgtatcgg gcaccggcaa
     1681 gaacgtcccg ctgattgcga tggcgcacct ggttccgtac gtggccaccg ccagtgtcgc
     1741 caacctgcgc gacctcgagg acaaggtgac gcgggccatg tcgatccgcg gcgcgcgcta
     1801 cgtacacatc cacgtgccgt gcccgctcgg atggggctcg gtgccggccg acacgatcaa
     1861 ggtggcgcgt gcggccgtcg agtcggggct cttcccgctg ttcgaggccg aacacggcga
     1921 agtcaccgga cgtacgccga tacgtcatcg cgtcccggtt gatgactacc tgaagctgca
     1981 gaagcgcttc gcgcacctgt tcagtccgaa gcgcgatgag gcgcgtctgg cgcagatcca
     2041 ggccatcgcc gaccgcaaca tctcgaccta cggtctgcta gacctcgagg gtgcccgtgg
     2101 ataagccgtt tgccatcacc ctcgacccgg gctcgtcgct ggcgaacctc accggctcgt
     2161 ggcgtacgtc gcgtccggtc tatgtcgacc ggctgccccc gtgcaacaac gcctgcccgg
     2221 cgggcgagaa catccagcag tggctgtacc acgccgaggg cggtgactat caggctgcgt
     2281 ggcgccagct ggtgcaggac aacccgctgc cttccatcat gggtcgcgtc tgctaccacc
     2341 cgtgcgagac cgcgtgcaat cgtgcccaca tcgacgaggc tgtcggcatc aactcggtcg
     2401 aacgcttcct cggcgacgaa gcgctcaagc gccactggaa gttcgatccg ccgacgaagg
     2461 aaacaggcaa gcgtgtcctc gtcgtcgggg ctgggccgtc gggcctgtcg gcggcgtatc
     2521 acctgcgtcg gcgcggtcat gcagtgacga tctacgaagc tgggccgttg gccggcggca
     2581 tgatgcgctt cggcatcccg acctaccgcc tgccacgcga tgtcctcgat gccgaagtgc
     2641 gccgcatcct tgacctgggt gtcgaactca agctgaaccg caaggtttcg aacattcttg
     2701 atgagatgcg cgagggcggt ttcgacgccg ccttcctcgc ggtgggtgcg cacattgcga
     2761 agcgcgccta catcccggcc ggcgaggccg cccacatgct cgacgcggta tcggtcctgc
     2821 gcagcatgga aggcgaggac aagccgatgc tcgggcgccg cgttgttgtc tacggcggcg
     2881 gcaataccgc gctggacgtc gcgcggaccg ccaagcgcat gggcgccgag ccgatcatcg
     2941 tttaccgccg tacgcgcgag aagatgccgg cgcacgactt cgaggtcgaa gaggcgctcg
     3001 ccgagggcgt gctgatcaag tggctgtcga cgatccgcaa cgtcgacgac agcggcacga
     3061 tgactgtcga gaagatggcg ctcgacgaga agggcttccc gcagccgacc ggcgagttcg
     3121 agaccctcga ggccgactcg ctcgtgctg
//
LOCUS       DATIRT010000069         4545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_106049, whole genome shotgun sequence.
ACCESSION   DATIRT010000069 DATIRT010000000
VERSION     DATIRT010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106049"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VLN25_01705"
     CDS             <1..843
                     /locus_tag="VLN25_01705"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSQ09319.1"
                     /translation="DNIASPLRVAGVNAAQTKERVGRIAELLQLSPLLDRRPSELSGG
                     QQQRTALARALVKDADLVLLDEPLANLDYKLREGLRDELPRLFADRECTVVYATTEPA
                     ETLLLGGHTATLHEGAITQFGPTQEVYRRPADLTTAQVFSDPPINTAPAIKRGSRIEI
                     SNVAGWNVDGRLAALPDGAYTFGLRPHHVQPAESGAQAGMQVDPISDGVRISGRVSVA
                     EISGSESVVHFDLHGLGWVSQSHGVHRFAVGAVADFRLDVEHGLYFDADGRCVSAGGN
                     AMAG"
     gene            846..1931
                     /locus_tag="VLN25_01710"
     CDS             846..1931
                     /locus_tag="VLN25_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSQ09320.1"
                     /translation="MARIGLKNVRHSYRERPAADADWAVKRLNVEWAEGSANALLGPS
                     GCGKTTLLNIISGLLRPTEGTVFFGDRDVTTAPPDARNIAQVFQFPVVYDTMSVFDNL
                     AFPLRNRGVPGAQVEKRVREIAALLDLEPMLMQRAAGLTADGKQKISLGRGLVRDDVN
                     VIMFDEPLTVIDPHLKWRLRGKLKELHQRLRPTMIYVTHDQTEALTFADQVLVMNDGT
                     VVQAGTPVELFERPRHTFVGHFIGSPGMNVLPATYENGVARFAGHAVETAAPLNVPQD
                     GARLEIGVRPEFVQFAEKGIAVRILKVADSGRYRIVDTMADAQPPSTATIIKLLVAEG
                     TALPSERAFLKFDPAHTQLYVDGWIAG"
     gene            1935..2801
                     /locus_tag="VLN25_01715"
     CDS             1935..2801
                     /locus_tag="VLN25_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HSQ09321.1"
                     /translation="MNNKTVNHKAWWLVVPVVLLVAFNAVIPLMTVVNYSVQETFGEN
                     VFFFEGVKWFEQILRSPRFHESLQRSLLFTAIILVIEVPLGVAIALAMPRKGPWVSVC
                     LVLMAMPLLIPWNVVGAMWNILALPDIGLLGYTLNAMGIEYNFTRQPVAAWATLIAMD
                     VWHWTSLVVLLAYAGLSAIPDAYYQAAKIDGASRWAVFRYIQLPKMKRVLTIAILLRF
                     MDSFMIYTEAIVVTGGGPGNSTTVLSIDLVKIALGQFDLGPAAAMSLIYFLIILLLSW
                     IFYTVMTKDEQS"
     gene            2798..3598
                     /locus_tag="VLN25_01720"
     CDS             2798..3598
                     /locus_tag="VLN25_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HSQ09322.1"
                     /translation="MKRYAWLLPFVYILFVLAPIYWLLNMSFKTTNEILGSFTLWPNE
                     FTFDNYRKIFTDSTWYNGYLNSIAYVLMNTVISVTVALPAAYAFSRYRFLGDKHLFFW
                     LLTNRMAPPAVFALPFFQLYSAIGLFDTHIAVALAHCLFNIPLAVWILEGFMSGVPKE
                     LDETAFVDGYSFWRFFIRIFIPTIAAGIGVAAFFCFMFSWVELLLAKTLTSVAAKPIA
                     ATMTRTASTSGYELGLLAAAGALTIVPGAFVIYFVRNYIAKGFALGRV"
     gene            3648..3914
                     /locus_tag="VLN25_01725"
     CDS             3648..3914
                     /locus_tag="VLN25_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2160 domain-containing protein"
                     /protein_id="HSQ09323.1"
                     /translation="MAWTWQTAAFFIVIGLLLVGMAVWEWRAPGGAPRRGVLGIVTTR
                     GDRLFISLLLAAYVHLIWLALVAAPLWGATLLSTGLAVAVFRKV"
     gene            complement(3969..>4545)
                     /locus_tag="VLN25_01730"
     CDS             complement(3969..>4545)
                     /locus_tag="VLN25_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09324.1"
                     /translation="LLLIASLVALFRIRAFRFLLRLLAGLMAIAVAAFAATVGIGMQG
                     YRALTHEAVAAQIRVHPMAPQRFSATLRFPDGREATYELAGDEIYIDAHILKWHPIDN
                     QFGLHTAYELDRISGRYHSLLDEKAAPRTVYALGTDRLIDLFNLRRRYAFLAPLLDAE
                     YGSASFVPVTAPAELEVRISTTGVLIRERKP"
BASE COUNT          765 a         1404 c         1496 g          880 t
ORIGIN      
        1 gacaacatcg cctcgccgtt gcgcgttgcc ggcgtaaatg ccgcgcagac gaaggaacgg
       61 gtcggacgca tcgccgaatt gctgcaactt tcacctttgc tcgaccggcg accgtcggag
      121 ctatcgggcg gccagcagca acgcacggct ctggcacgtg cgctggtgaa ggacgccgac
      181 ctggtactgc tcgatgagcc gctggccaac ctcgattaca agttgcgcga aggtctgcgt
      241 gacgaacttc cgcggctgtt cgccgatcgt gagtgcacgg tcgtgtacgc aacgacggaa
      301 ccggccgaga cactgctgct aggcggccac acggcgacgc tgcacgaggg cgcaatcacc
      361 cagttcggcc cgacgcagga ggtttatcgc cgcccggccg acctcacgac cgcgcaggtg
      421 ttttccgatc caccgatcaa tacagcgccg gcgatcaagc gcggctcgcg gatcgagatc
      481 agcaacgtcg cgggctggaa cgtcgatggt cgactggcgg cgctgcccga cggcgcctac
      541 acgttcgggc tgcggccaca tcatgtacag ccagcggagt ccggtgcaca agcgggcatg
      601 caggtcgacc ccatttcgga cggcgtccgg atcagcggac gggtgtcggt cgccgagatc
      661 agcgggtcgg aaagcgtggt gcacttcgac ctgcacgggc tcgggtgggt gtcgcagtcg
      721 cacggcgtgc accgattcgc cgtcggcgcg gtggccgact tcagactcga cgtcgagcac
      781 ggcctgtatt tcgatgccga cggccgctgt gtcagcgcgg gcggcaacgc catggcgggg
      841 tagcgatggc gcggatcggc ttgaagaatg ttcggcacag ttatcgtgag cgtccggccg
      901 ctgatgcgga ctgggccgtc aagcgtctga atgtcgaatg ggccgaaggc agcgccaatg
      961 cgctgcttgg cccttcgggc tgcggcaaga ccacgctgtt gaacatcatt tcgggcctgt
     1021 tgcgacccac cgaagggacg gtgttcttcg gtgatcgcga tgtgaccact gcgccgcccg
     1081 acgcgcgcaa catcgcgcag gtgtttcagt tcccggtcgt gtacgacacg atgagcgtgt
     1141 tcgacaacct ggcctttccg ctgcgcaatc gtggcgtgcc cggcgcccag gtagaaaaac
     1201 gcgtgcggga aatcgccgca ctgctcgatc tggagccgat gctgatgcag cgcgcggcgg
     1261 gcctgacagc cgacggcaag caaaagattt ctctcggacg gggactggtg cgcgacgacg
     1321 tgaacgtgat catgttcgac gaaccactga ccgtgatcga tccgcatctg aagtggcggc
     1381 tgcgcggcaa gctcaaggaa ctgcaccagc ggctgcgccc gacgatgatc tacgtgaccc
     1441 acgaccagac cgaggcgctg acgttcgctg atcaggtgct ggtgatgaac gacggcaccg
     1501 tcgtgcaggc cggcacgccg gtggaactct tcgagcggcc gcggcacacc ttcgtcggcc
     1561 acttcatcgg ttcgcccgga atgaacgtgc tgccggccac ctacgagaac ggagtcgctc
     1621 gattcgccgg ccacgcggtc gagaccgcgg cgccgttaaa cgtgccgcag gacggcgcgc
     1681 ggctcgagat cggggttcgt ccggagttcg tgcagtttgc ggaaaagggc atcgccgtgc
     1741 gcattctcaa ggtggccgac agcggccggt accgcatcgt cgatacgatg gcagatgctc
     1801 agccgccgtc gaccgcgacg atcatcaagc tgctggtggc tgagggaacg gcgctgccgt
     1861 cggagcgcgc atttctgaag ttcgatccgg cgcacacaca gttgtacgtc gacggctgga
     1921 tcgcaggctg agtcgtgaac aacaagaccg tcaatcacaa ggcctggtgg ctggtcgtgc
     1981 cggttgtgtt gctggtcgcg ttcaacgcgg tcatcccgtt gatgacggtc gtcaactact
     2041 cggttcagga aaccttcggc gagaacgtgt tcttcttcga aggcgtcaag tggttcgagc
     2101 agatcctgcg ttcgccgcgg tttcatgaat cgctacagcg gtcgttgtta ttcaccgcga
     2161 tcattctcgt gatcgaggtg ccgctcggcg tggccatcgc attggcgatg ccgcgcaagg
     2221 gcccgtgggt ctcggtgtgc ctggtgctaa tggcgatgcc gctgctgatt ccctggaatg
     2281 tcgtcggtgc gatgtggaac atcctcgcgc tgcccgatat cggcctgctc ggttacacct
     2341 tgaatgcgat gggaatcgaa tacaacttca cgcgccagcc ggtcgccgcg tgggccacgc
     2401 tgatcgcgat ggatgtctgg cactggacct cgctggtcgt gctgctcgcc tacgcgggcc
     2461 tgtccgcgat tccggacgcc tactaccagg ccgcgaagat cgacggtgca tcgcgctggg
     2521 cggtgttccg ctacatccag ctgccgaaga tgaaacgtgt gctgacgatc gcgattctgc
     2581 tgcgcttcat ggacagcttc atgatctaca cggaggcaat cgttgtcacc ggcggcgggc
     2641 cgggcaactc gaccaccgtg ctgtcgatcg acctggtcaa gatcgcgctc ggccagttcg
     2701 acctcgggcc ggcggcggcg atgtcgctga tctacttcct gatcatcctg ttgctgtcgt
     2761 ggatcttcta cacggtcatg acgaaggacg aacagtcgtg aagcgctacg cctggctgct
     2821 gccctttgtc tacattctgt tcgtgctggc gccgatttac tggctgctga acatgtcgtt
     2881 caagaccacg aacgagatcc tcgggtcatt cacgctgtgg cccaacgagt tcaccttcga
     2941 caactaccgc aagatcttca ccgactcgac ctggtacaac ggatacctca attcgatcgc
     3001 gtacgtgctg atgaacacgg tgatttcggt caccgtcgcg ctgccagcgg cgtacgcgtt
     3061 ctcgcgctac cgcttcctcg gcgacaagca cctgttcttc tggctgctga ccaaccggat
     3121 ggcaccgccc gccgtgttcg cactgccgtt cttccagctg tattcggcaa tcggattgtt
     3181 cgacacgcac atcgcggtgg cgctggcgca ttgcctgttc aacattccgc tggcggtctg
     3241 gatcctcgag ggcttcatgt cgggcgtgcc gaaggagctc gacgagaccg cgttcgtcga
     3301 cggctattcg ttctggcgct tttttatccg catcttcatt ccgaccattg ctgccggcat
     3361 cggggtcgcg gcgttctttt gtttcatgtt ctcgtgggtg gagctgctac tggccaagac
     3421 gctcacgtcg gtggcagcga agccgatcgc ggcaacgatg acgcgcactg cgagcacctc
     3481 gggttacgag ctgggactgc tggccgcggc gggcgcgctg acgatcgtgc cgggtgcgtt
     3541 cgtcatctat ttcgttcgca actacatcgc caagggcttt gccctcgggc gcgtttgaga
     3601 tttgagccat gacgccagcg cctactccag gaatcggact cggatggatg gcgtggacgt
     3661 ggcagaccgc cgcgttcttc atcgtcatcg ggctgctgct ggtcggcatg gcggtgtggg
     3721 agtggcgtgc acccggcggg gcaccgcgtc gcggtgtgct gggcatcgtc acgacgcgcg
     3781 gcgaccggct gttcatttcg ttgctgttgg ccgcctacgt tcacctgatc tggctcgcgc
     3841 tggtcgccgc gccgttgtgg ggggcgacgc tgctgtcgac ggggcttgct gtggcagtgt
     3901 tccgcaaagt ctgagcggaa ggaggcgact cagctcgcca ggatggcacg accggtcgcg
     3961 atcggcgcct aaggcttgcg ctcccggatc agcacgcccg tggtcgagat cctgacctcg
     4021 agctccgccg gcgcggtgac cggcacgaac gaggccgaac cgtactcggc gtccagcagc
     4081 ggtgcgagga aggcgtagcg ccggcgcagg ttgaagagat cgatcagcct gtcagtgccg
     4141 agcgcataca cggtgcgcgg cgccgctttc tcgtcaagca gcgagtgata ccgccctgag
     4201 atgcgatcga gctcgtaggc ggtgtgcagc ccaaactggt tgtcgatcgg atgccacttc
     4261 aggatgtgcg catcgatgta gatctcgtcg cctgccagct cgtacgtcgc ctcgcggccg
     4321 tccgggaagc gcagcgtggc gctgaatcgc tgcggggcca tcgggtggac gcgaatctga
     4381 gccgcaactg cctcgtgcgt caacgcccgg tagccctgca tgccgatgcc gaccgtcgcg
     4441 gcgaaagcgg cgactgcgat cgccatcaac ccagccagca gccgcagcag gaatcggaac
     4501 gcgcgtatcc ggaacagcgc aaccaggctc gcgatgagca gcagc
//
LOCUS       DATIRT010000070         2399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_598210, whole genome shotgun sequence.
ACCESSION   DATIRT010000070 DATIRT010000000
VERSION     DATIRT010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598210"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2197
                     /gene="infB"
                     /locus_tag="VLN25_01735"
     CDS             <1..2197
                     /gene="infB"
                     /locus_tag="VLN25_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HSQ09325.1"
                     /translation="AQRDAVRAAKEAVAAQQRARRAVEDEVAAIKSMMSAPKKVVKPA
                     EPAATAATAGTLHKPTTKPESKPGEGKKDDKKPGVKEIKSGKLASSWSEEQQKKRQLK
                     TRGDSSGGTGAWRGPKGHRKHGRDESDGAAQAMPTEPTVRDVSVPETITVAELAHKMS
                     VKAAEVIKVLMKLGQMVTINQVLDQDTAMIIVGELGHNPIAAKLDDPEALLAEREATA
                     TKSTGPEAVPEPRPPVVTIMGHVDHGKTSLLDYIRRTKVAAGEAGGITQHIGAYHVET
                     PRGIITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTKEAIAHAKAAGVPL
                     VVALNKMDKPEANPDRVKQELVANGVVPEDYGGESPFIQVSAKTGKGVDELLENVLLQ
                     AEVREFKAIKDGPAKGLVIESQLDKGRGPVATVLVQSGTLKRGDVVLVGSSFGRVRAM
                     LDETGKPIQEAGPSIPVEIQGLSDVPAAGDELIVLGDERKAREIALFRQGKFRDTKLA
                     RQQAAKLENMFENVGEGAARTLPLIIKADVQGSQEALTHALTKLSTDEVKVQVVHAQV
                     GGITESDVNLALASKAVIIGFNVRAEVSARKLAEGNGIDIRYYNIIYDAVDEVKAALS
                     GLLSPEQREQVLGTVEIRQIFRVPKIGAVAGCMVLDGLVKRTASARLLRDSVVIWTGE
                     LDSLKRFKDDVKEVKAGFECGLSLKGYDDIKEGDQLEIFEVQEVARTLL"
     gene            2291..>2399
                     /locus_tag="VLN25_01740"
     CDS             2291..>2399
                     /locus_tag="VLN25_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008164155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HSQ09326.1"
                     /translation="MKPTRKPGRGLRIADQIQRDLAEMIQREVPTGRAGL"
BASE COUNT          515 a          730 c          813 g          341 t
ORIGIN      
        1 ggcgcaacgt gacgcggtcc gtgccgccaa ggaggcggtc gctgcacagc aacgcgctcg
       61 ccgcgcagtt gaagacgaag tcgccgccat taagtccatg atgtcggcgc ccaagaaggt
      121 ggtgaagccg gccgaacccg ccgctacggc agcgacggca ggtacgttgc acaagcccac
      181 aaccaagccc gaatccaagc cgggcgaagg caagaaggac gacaagaagc cgggcgtcaa
      241 ggaaatcaag agcggcaagc tcgcgtccag ctggtccgaa gagcagcaga agaagcgtca
      301 gctcaagacg cgcggtgact cgtctggagg caccggcgcc tggcgcggac ccaagggaca
      361 tcgcaagcat ggtcgcgacg aaagcgacgg tgcggcgcag gctatgccga ccgagccgac
      421 ggtgcgggac gtctcggtgc cggagacgat caccgtggcc gaactggccc acaagatgtc
      481 cgtcaaggcg gccgaggtca tcaaggtgct gatgaagctt ggccagatgg tcacgatcaa
      541 ccaggtgctc gaccaggaca ccgcgatgat catcgtcggt gaactcggcc acaacccgat
      601 cgccgccaag ctcgacgatc ccgaggcgct gctcgccgag agagaagcca cggcgaccaa
      661 atcgaccggt cccgaggctg tgcccgagcc gcgtccgccg gtggtcacga tcatgggaca
      721 cgtcgatcac ggcaagacct cgctgctcga ctacatccgc cgtaccaaag tggcggcggg
      781 cgaagcgggc gggatcacgc agcacatcgg tgcctatcac gtcgaaacgc cgcgcggcat
      841 catcacgttc ctcgacacgc ccggccacga agccttcacg gcgatgcgcg cgcgtggtgc
      901 caaggcaacc gacatcgtca tcctggtggt ggcggccgat gacggcgtaa tgccgcagac
      961 caaggaggcg atcgcgcacg cgaaggccgc cggagtcccg ctggtcgtgg cgctgaacaa
     1021 gatggacaag cccgaagcca acccggaccg ggtcaagcag gaactggtgg ccaacggagt
     1081 cgtccctgag gactacggcg gcgagtcgcc gttcatccag gtgtcggcga agaccggcaa
     1141 gggcgtcgac gagctgctgg aaaacgtgct gctgcaggcc gaagtgcgcg agttcaaggc
     1201 gatcaaggac ggcccggcca agggcctcgt gatcgagtca cagctcgaca agggccgagg
     1261 cccggtcgcc acggtgttgg tccagagcgg cacgctcaag cgcggcgatg tcgtgctggt
     1321 gggctcgagc ttcggccgtg tccgcgcgat gctggacgaa accggaaagc cgatccagga
     1381 agcaggcccg tcgatcccgg ttgagatcca gggcctgtcg gatgtgccgg ccgcaggcga
     1441 cgaactcatc gtccttggcg acgagcgcaa ggcgcgcgag atcgcgctct ttcgccaggg
     1501 caagttccgc gacaccaagc tcgccaggca gcaggccgcc aagctcgaaa acatgttcga
     1561 gaacgtgggc gagggtgctg cacggacatt gccgctgatc atcaaagccg atgtccaggg
     1621 ttcacaggag gcgctcaccc atgcgctgac gaagctgtcg accgacgagg tcaaggtcca
     1681 ggtggttcat gcgcaggtag gcggcatcac ggaaagcgat gtcaacctgg cgctcgcgtc
     1741 gaaggcggtg atcatcggtt tcaacgtgcg cgcggaagtc tcggcacgca agctggccga
     1801 gggcaacggc atcgacatcc gctactacaa catcatttat gacgcggtcg acgaggtgaa
     1861 ggcggcgctg tcaggactgc tgtcgccgga acagcgcgag caagtgctgg gaaccgtcga
     1921 gatccgtcag atcttccgtg taccgaagat cggcgcggtg gccggctgta tggtgctcga
     1981 cggcttggtc aagcgcacag cgagcgcgcg cctgctgcgc gacagcgtgg tcatctggac
     2041 cggcgaactc gattcgctga agcgcttcaa ggacgacgtc aaggaagtca aggcgggctt
     2101 cgaatgcggc ctcagcctca agggctatga cgacatcaag gaaggcgatc agctggaaat
     2161 cttcgaagtc caggaagtcg cccggaccct gttgtagcaa tgccggcgaa agtacaaacc
     2221 tcctttcgct gggtcgagtt tgtaaaagtt cgctggcaga gccagacgaa ctttttgatg
     2281 gcacctgcat atgaaaccaa ccaggaagcc cgggcgcggg ctgcgcatcg cggatcagat
     2341 ccagcgcgac ctcgcggaga tgatccagcg tgaggtgcct acggggcgcg ccggcctga
//
LOCUS       DATIRT010000071         9225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1188166, whole genome shotgun sequence.
ACCESSION   DATIRT010000071 DATIRT010000000
VERSION     DATIRT010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9225
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188166"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VLN25_01745"
     CDS             complement(<1..195)
                     /locus_tag="VLN25_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09327.1"
                     /translation="MSARNQFALLSERRFAPLFWVQFLGAANDNILYWRAGLRKFSPA
                     WFVAIHAPVPLVFGLRFALGL"
     gene            complement(585..1595)
                     /locus_tag="VLN25_01750"
     CDS             complement(585..1595)
                     /locus_tag="VLN25_01750"
                     /inference="COORDINATES: protein motif:HMM:NF016915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSQ09328.1"
                     /translation="MIIYRAFESPKVRALYARGITFGALLASLLSGCVFLRPPDLDPT
                     PLEAGQLPAADISVQLPQLGPCTDAPQRAFSLDSSKPVTVLVHGCKGSAGRFRSLAQL
                     YAFHGQQAVCFSYDDRASLVQSSEQLAAAVGALAGRMRNRDITVIGHSMGGLVARKAM
                     EGERRAEWEGERVNVNLVTVSAPVSGIAVATPCGYRTLHWASLGTVPLTCWAITGANW
                     YEITASSDFIRRPGPLLASVRRYVKVVTDERGACRRRDAAGACVESDDIFSLAEQYQP
                     VIDGYPRLTNVQVASGHVEIVGYKGVAPRQLVSILQQQGLLAATAVGREAALGRLLAE
                     LY"
     gene            complement(2371..3015)
                     /locus_tag="VLN25_01755"
     CDS             complement(2371..3015)
                     /locus_tag="VLN25_01755"
                     /inference="COORDINATES: protein motif:HMM:NF016595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HSQ09329.1"
                     /translation="MATFHGIYSASVLDNRDPENLARVLVRLPGLKDASNDVWARLGT
                     MMAGRDSGTLFIPEVGDEVLVAFVQGDLRAPCVIGALWSKATPPPAVGDPPASVMLIR
                     SHNGVTLRIHDDKDNKSLTVETPGGQRITLQDDPGTVRIEDTHGNSVTMSPSGMKVLA
                     AAKVEVSAGIAEISAGLLTVHAGLSRFDGVVTCDTLISNAVVSASYTPGAGNVW"
     gene            complement(3192..3815)
                     /locus_tag="VLN25_01760"
     CDS             complement(3192..3815)
                     /locus_tag="VLN25_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HSQ09330.1"
                     /translation="MRFEGTLKSWNDERGFGFIAPDQGSDEVFVHVKAFSRRNGRPAV
                     GQRLVFEVEIGPQGKKRAKNVEAARIAVAKRSRREEEPADWDFASLSAIPVFVAVYVF
                     ASFYWKVSPLWAVAYFVASIVTLFAYAFDKTAAVRGNWRIQEGTLLGLGLLCGWPGAL
                     LAQKGLRHKSSKQSFRNAFWITVLLNLLTFIALNSPQISPQLKHLVR"
     gene            complement(4114..5901)
                     /locus_tag="VLN25_01765"
     CDS             complement(4114..5901)
                     /locus_tag="VLN25_01765"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSQ09331.1"
                     /translation="MTPQRLRLALAVAAACVLALNPSSTLGGGADFDPLLADARSCVP
                     AAGGPPPLFRKLVVAATETAPFQPVPAKPALADAPVLYGNLGTVRLKAGTGNARAQAW
                     FDQGVRLMFAFNHAEAIRAFREAQKLDPNCALCYWGEALAYGPNINVPMMPEANAPAL
                     SALAKAVELAPSAPRRDHDLIAALAHRYSADPKAERAALDAAYAEAMKQVAARYPAEN
                     TIQTLYVEAMMDTQPWDYWEAGGTKPKGHGAQIVSVLETVLKRNPSHPGAIHLYIHAV
                     EASTTPERALPHANRLGALMPGAGHIVHMPAHIYYRVGMYRESLETNKRAMAVDERYF
                     ASSPSDPLYKAAYYPHNIHFLMVSAQMGGDGQAAIAAAQKLDASMAPEVVAQFAIMQP
                     VKAAPYTTHAQFSSPDTILALPAPAPGLALVQTMYHYARAVAFAQKKDFASAQKEIDA
                     LASIEKDADFKPFAEWGIPAKEIVQTARLVAAGRMADAKGDLDGAARAYEDAVFIEDA
                     LVYTEPPYWYYPVRQSLGAVRLRQGKLDEAEKAFRDSLGRVRNNGWALAGLAETYKRK
                     GDVGGEKAARRAYAKTWLGGSKGPAIEQL"
     gene            6248..8521
                     /locus_tag="VLN25_01770"
     CDS             6248..8521
                     /locus_tag="VLN25_01770"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HSQ09332.1"
                     /translation="MSDNLREAALEYHRLPTHGKISVVPTKAMVTQRDLALAYSPGVA
                     EACNEIVRDPREAHTLTARGNLVAVVTNGTAVLGLGNIGPLAGKPVMEGKGCLFKKFA
                     GIDVFDIELDEHDPDKLVDIIAALEPTFGGINLEDIKAPECFYIERKLRERMKIPVFH
                     DDQHGTAIVAAAAVLNGLRCVGKDIAQAKLVCSGAGAAAIACLDLLVSLGVRKENILV
                     TDSKGVLHADRKEGMDASKAQYARKTPARTLADAMPGADIFLGLSGPGVLKPEMVKSM
                     GAQPIILAMANPTPEIMPEEAKAVRPDAIIGTGRSDYPNQVNNVLCFPFIFRGALDVG
                     ATTINEAMKLATVRALADLTHAEIPDEVASAYGAEGLRFGPEYLIPKPFDPRLIDRIA
                     PAVAQAAMDSGVAMRPMADVEAYRAQLRRFVYQSGSTMEPVFSAAKKSPRRLIYAEGE
                     EERILRAVQVVVDEGLAKPILIGRPDVIETRIERYGLRLKLGRDAECVNVLDDPRYRD
                     TWSDYYQLTKRKGVTRSLAQAEVRTRTTLIGAMLVRNRDADAMLCGTVGAYADHLRYV
                     RNVIGLRAGAQTFAAMQLLILPNRQLFICDTHVNADPTAEQIAEMTMLAADAVCRFGI
                     APRVALLSHSNFGSSELPSAQKMRAALELVRARKPDFPVDGEMQADTALSHAVLEGSM
                     PDSPLEGEATLLIMPNVDAANITYNALRVTAGGGITIGAILLGAAQPVHILTPASTVR
                     RIVNMSAVAVVDALARG"
     gene            8578..>9225
                     /locus_tag="VLN25_01775"
     CDS             8578..>9225
                     /locus_tag="VLN25_01775"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSQ09333.1"
                     /translation="MAFDFMNKRVVVTGGSRGIGRAIALGFAAAGAGVSICARHPDVL
                     ERTRVEIAGKGHRTHAAVCDLADGPAVQRYVDEAGAELGGIDVLVNNASGFGMGDDEA
                     GWAAGLSIDIMAVVRASRAALPLLETAPGSSIINISSISGFRASTRAPAYGAVKVAMM
                     HYTASQAATLARKGIRVNCIAPGSIEFPGGHWEGRRLAKDPLYSQVLSKIPFGRMG"
BASE COUNT         1660 a         3003 c         2951 g         1611 t
ORIGIN      
        1 caggccgagg gcgaagcgca agccgaatac cagcggcacc ggcgcgtgga tcgcgacgaa
       61 ccaggccggc gagaacttgc gcaggcccgc tcgccaatat aagatgttgt cgttggccgc
      121 gccgagaaac tggacccaga acagcggcgc gaagcggcgc tcgctcaaca gggcaaactg
      181 gttccgcgcg ctcacacggc gcctatttcg ggtgtagttg atgtcacgcg gattggcggg
      241 ccgggtcagg cgacgcggcc attatgtcag ccggcttccc ggcccagctc acaaaaattc
      301 tcgaatggcc gtgcgcaagc gacgtctgct tgcggagggc gacgaagaca actcgcgacc
      361 cattgcggcc atcagcgctt tagaaaagca gccgccgatt tcacgctgaa agctgacatg
      421 cgcgcaatcg gcagcacgcg atcccaagct gccttcggcg gattcctaaa gcggtcgcga
      481 ttaccggcgc cgaagcggac atacgccccg gttcaggcga cgcgtggctt cgtcgcgcgt
      541 cgcctgtaac cggcggttag aacgcatttg ctggtgcctc ggcttcaata gagctccgcc
      601 agaagccgcc cgagggcagc ctcgcgtccc accgctgtgg cggcgagcaa gccctgctgc
      661 tgcaggatcg agactagctg gcgcggggcg acacccttgt agcccacgat ctccacgtgt
      721 cccgaggcga cttggacgtt ggtgaggcgc gggtacccat cgatgaccgg ctggtactgc
      781 tccgcgaggc tgaagatgtc gtcgctttcc acgcaagcgc ccgccgcgtc gcgccggcgg
      841 caggcgccgc gttcatcagt gacaaccttc acgtatcgcc gcactgaggc gagcagcgga
      901 ccggggcggc ggatgaagtc ggaggaagcg gtgatttcgt accagttggc gccggtgatg
      961 gcccagcagg tgagcggcac ggtgccgagg ctcgcccagt gcagggtgcg atagccgcat
     1021 ggggtcgcga ccgcgatgcc agagaccggc gctgagaccg tcaccaggtt cacgttcacc
     1081 cgttcgccct cccactcagc gcggcgctcg ccttccatcg ctttcctcgc taccagccca
     1141 cccatgctgt ggccgatgac tgtgatgtcg cgattgcgca ttcttcccgc gagcgcaccc
     1201 actgcagcgg ctagttgctc ggagctctgc acgaggctgg cccggtcgtc atagctgaag
     1261 cagaccgcct gctggccgtg gaacgcgtag agctgcgcga gcgaccggaa ccgtcccgcc
     1321 gaccccttgc aaccatgcac cagcacggtc accggcttcg acgagtcgag ggagaaggca
     1381 cgctgcggcg catccgtgca gggccccaac tgcgggagct ggacgctgat gtccgcagcg
     1441 ggcaactggc cggcctccag gggcgtgggg tcgagatccg gcggccgcaa aaatacgcaa
     1501 ccggagagaa ggctcgcaag aagcgcaccg aatgttatgc cgcgagcata gagcgctcta
     1561 accttaggcg actcaaatgc acggtagatg atcatgcgct ctgacggttg agctcgcctg
     1621 cgggcgacag accgcgcagc gggctggcat acgtctggtg gaacaaaggg ttattcatca
     1681 cattgcaggc cctgtgccgg gctcggtgta gccctccggc ttccgcgccc cagcggcaaa
     1741 cattcaatta agcatgacct aggcgcgctc gcgtgcgcgt tccaacaaat cggaggcacg
     1801 agcgtagctc ctgctgtcga tcactcattc cggacgttac tagccgcacg aaggggaatc
     1861 tttcgtttgg cttcacccag tgaacatcaa tgtgcactgt gtcggaaacg atttcgtgaa
     1921 agacggtttc gattttccct agacgtcttt cgacgtcatt cgagatttcc tcgatgcact
     1981 cttctccctg cggcggcctc cactctgaac gttttccgtg tctgtatatg ggctgaccag
     2041 tcaacgaaac gactttgctt tccttactag acacgacgct ctccgaacgg gacgccagga
     2101 tggatcagta agctgctttg cacgatagcg atcgcgggta gtgttggaaa caggagtctg
     2161 ggcgagctgc ttgagcgggc acgggagtta cttgggtctg cgactcctgt ttgcgcgcga
     2221 atgagtcagt gcggcatgta acagaagtgt cccgcgatag cggccgcttt ctggcagatc
     2281 gcgtgcggcg cagcggactg gcagatcctg gccgacttcg ccctggaaga ttctgtctgg
     2341 gcggcagctc tggcagtagc tgcgtgacgc tcaccacaca ttgccagccc caggtgtata
     2401 gctcgcactc accacggcat tgctgattaa tgtgtcgcac gtcaccacgc catcgaaacg
     2461 cgatagccca gcgtggaccg ttagcaagcc cgcgctgatc tccgcgattc ctgcgctcac
     2521 ctctaccttc gctgccgcga gcactttcat gccggacggt gacatcgtca cggaattgcc
     2581 gtgggtgtct tcgattcgca ctgtgcccgg gtcgtcctgc agcgtgattc gttgcccgcc
     2641 tggcgtctcg accgtcaagc tcttgttgtc cttgtcgtcg tgtatgcgca acgtaacgcc
     2701 gttgtgcgac cggatcagca tgacgcttgc cggtggatcg ccaacggcgg gcggcggggt
     2761 tgctttgctc cacagcgcac caatcacgca cggcgcccgt agatctccct gcacgaacgc
     2821 cacgaggact tcgtcgccga cttcgggaat gaacaacgtg ccgctatcgc ggccagccat
     2881 catcgtcccc agtcgcgccc agacgtcgtt gctcgcatcc ttaaggccgg gtagccgcac
     2941 gagcacgcgc gctaggtttt cgggatcgcg attgtcgaga accgaagccg agtagatccc
     3001 atgaaaggta gccatgtgat gcctccctgc tggccgagcg gttacgtcgg ctgagtcgcc
     3061 ataattctca aatcaacacg tcagcttttg tggcgatttg tgcgtagaag cgaaacgaca
     3121 cgatctggcc gtgccaagac accggtctag gcctgccaag caagcaacac gcgaaacgaa
     3181 gtatgggctg atcacctgac gagatgcttg agttgcggag agatctgtgg ggagttcagc
     3241 gcgatgaatg tcagtaggtt cagcaaaacc gtgatccaga atgcgttcct gaatgattgc
     3301 ttactcgact tgtggcgcag ccccttctga gcgagcagcg cgccaggcca accacacaga
     3361 agaccgagtc cgagcagcgt gccctcctga atgcgccagt tgcccctgac cgccgccgtt
     3421 ttgtcgaacg cgtaagcgaa aagcgtgacg atgctcgcaa cgaaataggc gaccgcccac
     3481 aggggagaca ctttccaata gaaagacgca aagacgtaca cggcaacaaa gactggaatt
     3541 gcggacaggc tggcaaagtc ccagtcagcg ggctcttcct cacgtcttga gcgcttcgct
     3601 acggcgatgc gagctgcctc gacgttcttt gcccgtttct tgccttgcgg gcctatctct
     3661 acttcgaaca caaggcgttg gccaaccgct ggacgcccat tgcgtctgga gaaagccttg
     3721 acgtgaacga agacctcgtc gcttccctga tcaggggcga taaagccgaa gccacgttcg
     3781 tcgttccagg acttaagggt gccttcaaag cgcatggtgc gaacgcggcc gcggtctcac
     3841 tgggtttggt tggagctgcg gcaagttagc gacaaatgga gagcgcggca acgtccgctt
     3901 agcagctaac tggtggcgcg aggaacgccg caggttttgg ccgactcaag accccgcaag
     3961 cttcgaccta gcagcataga tagactggcc aaccgatggg gcagagcgca caacgcgcgt
     4021 gcgcgctcgc gccgccgacc cgagcgatct gcacaggcgc gaacaccgtc gccctgctcc
     4081 gccccgagct gcaggctcgc gacgctcgaa cgatcagagc tgctcgatcg ccggaccctt
     4141 ggaaccaccc agccaggtct tcgcataggc gcgccgggcc gccttctcgc ctccgacatc
     4201 acccttgcgc ttgtaggtct ccgccagccc ggcaagcgcc cagccattgt tgcgcacgcg
     4261 ccccagagaa tcgcggaacg ccttctcggc ttcgtccagc ttgccttgcc gcagtcggac
     4321 cgcgccgagg gactggcgca ccgggtaata ccagtacggc ggctcggtgt agaccagcgc
     4381 atcctcgatg aagacagcat cctcgtaggc gcgcgctgcg ccgtcgagat cacccttcgc
     4441 gtcggccatc cggccggcgg ccaccaggcg tgccgtttgt acgatctcct tcgccgggat
     4501 gccccactcg gcgaacggct tgaagtcggc atccttctcg atgctggcga gcgcatcgat
     4561 ctccttctgt gccgacgcga agtccttttt ctgcgcgaac gccaccgccc gcgcgtagtg
     4621 gtacatcgtc tgtaccaatg cgaggcccgg cgccggcgcg ggcagcgcca ggatcgtgtc
     4681 gggcgagctg aactgcgcat gcgtcgtgta tggcgcggcc ttcaccggct gcatgatggc
     4741 gaactgcgcc acgacttccg gtgccattga cgcgtcgagc ttctgcgctg ccgcgatcgc
     4801 ggcctggccg tccccaccca tctgcgccga gaccatcagg aagtgaatgt tgtgcgggta
     4861 gtaggcggct ttgtagagcg ggtccgacgg cgacgacgcg aagtagcgct cgtccaccgc
     4921 catcgcgcgc ttgttcgtct cgagcgattc acggtacatg ccgacccggt aataaatgtg
     4981 cgccggcatg tgcacgatgt gaccggcacc gggcatcagc gcgccgagcc ggttggcgtg
     5041 aggcagcgcg cgctcgggcg tcgtcgacgc ctcgaccgcg tggatataca gatggatcgc
     5101 tccgggatga ctcggattgc gcttgagcac tgtctccagc accgagacga tttgcgcgcc
     5161 gtgacccttg ggcttagtgc cgcccgcttc ccagtagtcc cacggttgcg tgtccatcat
     5221 cgcttcgacg tagagcgtct ggattgtgtt ttccgccggg tagcgcgcgg cgacctgttt
     5281 catcgcctcg gcatacgcgg catcgagcgc agcgcgctca gccttcgggt ctgccgagta
     5341 acgatgcgcc agtgcggcaa tgaggtcgtg gtcccgtcgc ggcgccgacg gtgccagttc
     5401 gacggctttt gcgagcgctg agagcgccgg cgcgttcgcc tccggcatca tcggcacatt
     5461 gatgttgggt ccgtaggcga gcgcctcgcc ccagtaacac agcgcgcagt tcgggtcgag
     5521 tttctgcgcc tcgcggaacg cgcggatcgc ctcggcgtga ttgaacgcga acatcaggcg
     5581 aacgccctga tcaaaccatg cctgcgcgcg cgcgttgccg gttccggcct tcagccgcac
     5641 ggtcccgaga ttgccgtaga gcaccggcgc atcggccagg gccggtttgg ctggcaccgg
     5701 ctggaacggg gccgtctccg tcgccgcaac gacgagcttc cgaaagagcg gcggaggtcc
     5761 cccggccgcc ggaacgcagc tgcgtgcatc cgccagcagc ggatcgaagt cggcaccgcc
     5821 gcccagggtc gacgacgggt tcagggcaag gacgcaggcc gcggcgacgg ccaacgcaag
     5881 gcgcaggcgt tgaggtgtca tcttccctcc ggtttgcggc gcccgagtgg gcaaggcgtc
     5941 gcttctacgg cgttgccgct ggatccggtg ttcgcatagc aaccgactcc gacgacagcg
     6001 cgcccaggtt agggcgtgca atgtggtcct cctgctaccc ccgtgtcaag tggcgcagac
     6061 gcaatttccg ggcggccggg gcaccgtcac gttcccggcc cgccacgggc ctgcggagcg
     6121 cgaacgcgat tggcgcaggg ctcacgattg gcgcggggct cacaacgccg taaccttgtc
     6181 caaaagctag atgacagaac agcgcgtgcc cacgagccgg ggcaacgcgc acaccgagga
     6241 gacctccatg tccgacaacc tgcgcgaagc ggcgctcgaa taccaccgcc tgccgacgca
     6301 cggcaagatc tcggtcgtcc cgaccaaggc gatggtcacg cagcgtgacc tggcgctggc
     6361 ttactcgccc ggcgtggccg aagcctgtaa cgagatcgtg cgtgacccgc gcgaggcgca
     6421 cacgctgacc gcgcgcggca acctggtcgc ggttgtgacc aacggcacgg cggtgcttgg
     6481 gctgggcaat atcggtccgc tggccggcaa accggtgatg gaaggcaagg ggtgcctctt
     6541 caagaagttc gctggcatcg acgtcttcga catcgaactc gacgagcacg accccgacaa
     6601 gctggtcgac atcatcgccg cactggaacc taccttcggc ggcatcaacc tcgaggatat
     6661 caaggctccg gagtgcttct atatcgagcg caagctgcgg gagcggatga agattccggt
     6721 gtttcatgac gaccagcacg gcactgcgat cgtcgccgcg gcggcggtgc tcaacggtct
     6781 gcgctgcgtc ggcaaggaca ttgcacaagc gaaactggtc tgctcgggcg caggcgccgc
     6841 cgcaatcgcc tgcctcgacc tgctggtgag cctcggagta cgcaaggaga acatcctcgt
     6901 caccgacagc aagggcgtgc tccatgctga ccgcaaggaa gggatggatg cgagcaaggc
     6961 gcaatacgcg cgcaagaccc cggcgcggac tctggctgac gcaatgcccg gcgccgacat
     7021 cttcctcggg ctgtcggggc ccggagtgct gaagcccgag atggtcaagt ccatgggcgc
     7081 gcagccgatc attctcgcga tggccaaccc gacgccggag atcatgccgg aggaagccaa
     7141 ggccgtccgg cccgacgcga tcatcggcac cggccgttcc gactatccga accaggtcaa
     7201 caacgttctg tgtttcccgt tcatttttcg cggcgcgctg gatgtcggcg cgaccacgat
     7261 caacgaggcg atgaagcttg cgaccgtgcg ggcgctggcc gacctcacgc atgccgagat
     7321 tcccgacgag gtcgccagtg cctatggcgc cgagggcctg cgtttcggcc ccgaatacct
     7381 gattcccaaa ccgttcgacc cgcgcctgat cgaccgcatc gccccggccg tggcgcaggc
     7441 cgcgatggat agcggcgtcg cgatgcgccc gatggcggac gtcgaggcct atcgcgcgca
     7501 gctcagacgc ttcgtctacc agtcgggcag cacgatggag cccgtcttct cggcggcgaa
     7561 gaagtcgccg cggcggctca tctacgcgga aggcgaggaa gagcgcatcc tgcgcgcggt
     7621 gcaggtggtg gtcgacgagg gcctggctaa gcccatcctg atcggaaggc ccgacgtgat
     7681 cgaaacgcgc atcgagcgct acggtctgcg gctgaagctc ggtcgtgatg ccgaatgcgt
     7741 caacgtgctc gatgatccgc gctatcgcga cacctggtcg gattattacc agctcacgaa
     7801 gcgcaagggc gtgacccgat cgctggcgca ggccgaagtg cgcacgcgca ccacgctgat
     7861 cggcgcgatg ctggtgcgca accgggatgc cgacgccatg ctgtgcggaa ccgtgggcgc
     7921 ctacgccgat catctgcgct acgtgcgcaa cgtcatcggc ctgcgcgccg gtgcgcaaac
     7981 gttcgcggcg atgcaactgc tgatcctgcc gaatcggcag ctcttcatct gcgacaccca
     8041 tgtgaatgcc gatccgaccg ccgaacagat cgccgagatg acgatgctcg ccgccgatgc
     8101 ggtgtgccgc tttggcatcg cgccccgcgt cgccctgctg tcgcattcga atttcggcag
     8161 ttcggaactg ccgtcagcgc aaaagatgcg cgcggcactg gaactcgtcc gcgcgcgcaa
     8221 gcccgacttc ccggtcgacg gcgaaatgca ggccgacacc gcgctgtcgc acgccgtgct
     8281 cgaagggtcg atgccggact cgccgctcga aggcgaggcg accctgctca tcatgccgaa
     8341 cgtcgacgcc gccaatatca cgtacaacgc gctgcgcgtg accgcgggcg gcggcatcac
     8401 gatcggcgcg atcctgctgg gcgccgcgca gccggtgcac atcctgacac cggcgtcgac
     8461 ggtccgacgc atcgtcaaca tgtcggccgt ggcggtcgtc gacgccctcg cgcgcgggtg
     8521 atcgaacgcg tccccaacct cctagagttc gacgacacca cccgaaacgg atccggcatg
     8581 gccttcgact tcatgaacaa gcgcgtcgtc gtcaccggcg gcagtcgtgg catcggtcgt
     8641 gcgatcgcgc tcggttttgc ggccgcgggc gcgggcgttt ccatctgcgc gcggcacccg
     8701 gatgtactgg aaagaacccg cgtcgagatc gccggtaaag gccaccggac gcacgctgcg
     8761 gtctgcgacc tggccgacgg tcccgccgtg cagcggtatg tggacgaagc cggcgcggag
     8821 ctcggcggca tcgacgtgct ggtcaacaat gcgtcgggct tcggcatggg cgacgacgag
     8881 gccggctggg cggctggact ttcgatcgac atcatggccg tggtgcgcgc ctcccgcgca
     8941 gcacttccgc tgctggagac cgcgcccgga agttcgatca tcaatatttc gtcgatctcg
     9001 ggattccggg cctccacccg tgcgcctgcc tacggtgcag tcaaggtggc gatgatgcat
     9061 tacacggcca gccaggcggc cacactggcg cgcaagggca ttcgcgtcaa ctgcatcgct
     9121 ccgggctcga tcgaattccc cggcggccac tgggagggac gcaggctcgc aaaggaccct
     9181 ctgtacagcc aggtgctctc gaagatcccg ttcggccgca tgggc
//
LOCUS       DATIRT010000072         7400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_106327, whole genome shotgun sequence.
ACCESSION   DATIRT010000072 DATIRT010000000
VERSION     DATIRT010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7400
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106327"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VLN25_01780"
     CDS             complement(<1..553)
                     /locus_tag="VLN25_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSQ09334.1"
                     /translation="MGRIADQVQAPVIDRAALLASLRESRPWDVLVIGGGATGLGIAV
                     DAASRGLRTALIEARDFSSGTSSRSTKLIHGGVRYLAQGNVKLVREELIERARLIANA
                     PALVRPLEFIVPCYRPLEREFMRIGLGLYDALAGTRGIGPTRWLSRAETISRLPTVRT
                     EGLDGGVSYWDAQFDDARLAIALM"
     gene            complement(541..2058)
                     /gene="glpK"
                     /locus_tag="VLN25_01785"
     CDS             complement(541..2058)
                     /gene="glpK"
                     /locus_tag="VLN25_01785"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HSQ09335.1"
                     /translation="MTATQRHGQRYVLALDQGTTSSRAIVFDQNGRIVAWAQKEFRQN
                     YPQPGWVEHDASEIWSTQLACAQSALRQSAIPALDVAGIGITNQRETTVLWDRSTGAP
                     IANAIVWQDRRTAEHCAALRERGVEERLRAKTGLVLDPYFSATKIGWLLDHVPGARAR
                     AERGELAFGTIDSWLIWNLTGGTVHVTDVSNASRTLLIDLATLEWDEELLQLFNVPRT
                     VLPRITSSSEIVGGSLPELFGAPIPIAGNAGDQQAATFGQACFAPGMAKNTYGTGAFM
                     LMNVGDAPQMSSNRLLATAGWRLGAGPGATYMLEGSVFVAGAVVQWLRDGLGLIERSE
                     HVEALARQVSDNGGVYLVPAFVGLGAPHWDPDARGLLIGLTRGTSRGHVARAALESIA
                     FQSADVLTAMQGDAHRPLTELRVDGGAARNDLLMQFQADLMGVPVVRPEVTETTALGA
                     AYLAGLATGVWSAIDELTGLWRAERRFEPMMSSDERESRRSQWRRAVDRSRRWAE"
     gene            2143..3123
                     /locus_tag="VLN25_01790"
     CDS             2143..3123
                     /locus_tag="VLN25_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HSQ09336.1"
                     /translation="MFGRLLAVVAFAVIALTPAHAQTWPAKAVRIVVPFPPGGTTDIV
                     ARSLGVELQRMWQHAVVIENRPGAGGNIGADAVAKSAPDGYTLLMGTVGTHAINAALF
                     AQSGAKMPFDPAKDFVPITLAAGVPNVMVINSKLPVNTVAEFIEYAKARPGQLNMASS
                     GNGTSIHLSGELFKTVTGIYMVHFPYRGSAPAVTDLIAGNMSVMFDNLPSELPHIKSG
                     RLKALAVTSRTRSPALPNVPTIEEAAGLKGFDASSWFGLFAPTGTPRAIVDKIQADVA
                     KALALPEVRERFLAQGADPGGSTPEQFAAFIRAETDKWTRVVKFSNAKVD"
     gene            3189..4058
                     /locus_tag="VLN25_01795"
     CDS             3189..4058
                     /locus_tag="VLN25_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09337.1"
                     /translation="MRAGGTIATSFGVIESATAYRERVAATIVAMLRDDPAIAAIGEG
                     GAAARSRADEFSDLDLMIVAPVAGAAAIFEIVEAALRTIAPITHTWRVEPPGFPDMVQ
                     RFYFLADAPRHFAVDCSVITPAGIAAFLERERHGEMIVWFDRDATLTSRPIDGETLAR
                     RRANRLAQLRGSVPVYSMLVDKELERGHALEAYGFYQVLLRALIELLGMRHRPDRFDY
                     SWRYVERELPRSAQALVARHAFVRDGKSLALLSASLVDEITGLLDTLTAAEAAADAAA
                     PVNAAAPGDRSSP"
     gene            complement(4021..5061)
                     /locus_tag="VLN25_01800"
     CDS             complement(4021..5061)
                     /locus_tag="VLN25_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="depolymerase"
                     /protein_id="HSQ09338.1"
                     /translation="MWWCGGRFLIIAAAAAWITIAAAAQRLPAFDAHSDRITVSGLSS
                     GAFMAVQLAVAHSARVNGVGVFAGGPYYCVGINPTRAESICMQGSPGAGSSIKDAERL
                     ATLRLIDSTQNLRGMRAWLLAGEADSRVVTPVVRANYEFFAHYNVSGAQFRVQPGLGH
                     GLPTPTQGVACDASASPFINRCGVDLVGEMLTSLHPGASKSPGPGPGPSQGRIVVFGQ
                     AEFVPTWRRWWAMSSLGATGYAFVPSQCEKNTKCRVHVALHGCHQGVTAVGEVFVRDA
                     GYNAWAAAHDLIVLYPQAQASQPTMFTWWLPFNPNGCWDWWGYTGTDYAVKTGVQVKA
                     IMAMIDRLAQPR"
     gene            5063..5698
                     /locus_tag="VLN25_01805"
     CDS             5063..5698
                     /locus_tag="VLN25_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HSQ09339.1"
                     /translation="MTSERRTPLLAALLIVTIIALAMSGWRPYDRITWFMEVAPVLIA
                     IPLMLATRERYPLTTLLYVLICAHALVLILGGAYTYARVTLGNWVQEAFGLSRNPYDK
                     LGHFMQGFVPAMIAREILVRGRFVHGRRMLAFLCVCVALAISAAYELVEWWAALAMGQ
                     GADEFLGTQGDPWDTQSDMFLAMVGAVTALVTLSELHDRQLAAIAPQQSRT"
     gene            5854..6144
                     /locus_tag="VLN25_01810"
     CDS             5854..6144
                     /locus_tag="VLN25_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09340.1"
                     /translation="MLMRVRERNNEIEIQFSGLGGRQHAVLTAFSGSADGDERLFDRA
                     KLESLKVRARADAMNIRLRAKSGESFDVSHLYRSLRRALVEGERTAFGSLAG"
     gene            complement(6164..6769)
                     /locus_tag="VLN25_01815"
     CDS             complement(6164..6769)
                     /locus_tag="VLN25_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09341.1"
                     /translation="MSHWRIGGLALMLTTGSALAQLQGPGLRVPIEAEHAVELSPGIG
                     LRGWIGGRPGLLASDGLVASSMVLADWHPLSNGFRLSGGLAYGSLRLEPLATSGFGSE
                     RYLRDPGLSAAGLDPRSWLARGNPYLGLGWGLGAGSRSGLYFSADLGVMYQRSNLSTW
                     GCPAGIPTALCGPDARALDAPSGLDEARFAPMMSLGVGLRF"
     gene            complement(6946..7338)
                     /locus_tag="VLN25_01820"
     CDS             complement(6946..7338)
                     /locus_tag="VLN25_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09342.1"
                     /translation="MKEREKKDRRGPPTGTRTGPRARAAQARTFGDPFKRAERPARGE
                     APRERMHAPVSGAIRLDPDVARVFRDSESVNEALRLVMRLARFASGRPSGPPRDRPPA
                     FRERSSGPPRRTERGDRPPGPRKPRFEE"
BASE COUNT         1284 a         2548 c         2385 g         1183 t
ORIGIN      
        1 gcatcagcgc gatcgcgagc cgcgcatcgt cgaactgggc gtcccagtag gacacgccgc
       61 cgtccaggcc ttcagtccgt acggtcggga gcctggagat cgtttcggca cgcgagagcc
      121 atcgcgtcgg accgatgccg cgcgtgcccg ccagcgcgtc gtacaggccg aggccgatgc
      181 gcatgaactc acgctccaga ggccggtagc acggcacgat gaactcgagc ggccgcacca
      241 gcgccggcgc gtttgcgatc aatcgtgcgc gctcgatcag ctcttcgcgc accagcttga
      301 cgttgccctg cgcgagataa cgcacgccgc cgtggatcag cttggtgctg cggctcgacg
      361 tgccgctgga aaagtcgcgc gcttcgatca gcgcggtgcg caggccgcgc gacgccgcat
      421 ccaccgcgat gccgagtccg gtcgctccgc cgccgatcac gagcacgtcc caggggcgcg
      481 actcgcgcag cgaagccagc agtgctgccc gatcgatgac cggcgcctgc acctggtcgg
      541 ctattcggcc catcgtcgcg atcggtccac ggcacgacgc cactgcgacc gccgggattc
      601 acgctcgtcg gaactcatca tcggttcgaa acgacgctcg gcgcgccaca ggccggtcaa
      661 ttcgtcgatc gccgaccaca ctccggtcgc cagcccagcc agataggccg cgccgagcgc
      721 ggtcgtttcc gtgacctccg ggcgcacgac cggcacgccc atgaggtcgg cctggaactg
      781 catcagcaaa tcgttgcgcg cagcgccgcc gtcgacgcgc agttccgtca gcggccggtg
      841 ggcgtcaccc tgcatcgcgg tcagcacatc ggccgactgg aacgcgatcg attcgagtgc
      901 ggcgcgcgcc acgtgcccgc gcgacgtgcc ccgcgtcagg ccgatcagca atccgcgcgc
      961 gtcggggtcc cagtgcggcg cgccgagacc cacgaaggcc ggcaccagat aaacgccacc
     1021 gttgtcgctc acctgccgtg caagggcttc gacatgctcc gaacgctcga tgagtcccag
     1081 cccgtcacgc agccactgca ccacggcgcc ggccacgaaa acgctgcctt cgagcatgta
     1141 ggtggcgccg ggcccggcac cgagccgcca gcccgcggtc gcgagcaacc ggttggacga
     1201 catttgcggt gcgtcaccga cattcatcag catgaaggcg ccggtgccgt aggtgttctt
     1261 cgccatgccc ggagcaaagc aggcctggcc gaaggttgcg gcctgctggt cgccggcgtt
     1321 gcccgcgatc gggatcggcg cgccgaagag ttcgggcaag gatcccccga cgatctcgct
     1381 cgacgacgtg atccgcggca gcaccgttcg cggcacgttg aagagttgca gcagttcttc
     1441 gtcccactcg agagtggcga ggtcgatcag cagcgtgcgc gacgcattgc tgacgtcggt
     1501 aacgtgaaca gtgcccccgg tcaggttcca gatgagccag ctgtcgatcg tcccgaaggc
     1561 cagctcgccg cgctcagcac gcgcgcgggc gccgggcaca tggtcgagca gccacccgat
     1621 tttggtcgcg gaaaagtacg ggtccagcac caggccggtc ttcgcccgca ggcgctcctc
     1681 cacgccgcgt tcgcgcaacg cggcgcagtg ctcggcggtg cgccggtcct gccagacgat
     1741 cgcattggcg atcggcgcgc cggtggagcg gtcccacagc accgtcgttt cgcgctggtt
     1801 ggtgatgccg atgcccgcca cgtcgagggc cggaatcgcg gattgccgca gtgccgactg
     1861 tgcgcaggcc agctgcgtcg accagatctc gctggcgtcg tgctcgaccc agccgggctg
     1921 cggatagttc tggcggaact ccttctgcgc ccacgccacg atccggccgt tctgatcgaa
     1981 gacgatggcg cgcgaactcg tcgtaccctg gtcgagtgcc agcacgtagc gctggccgtg
     2041 tcgttgcgtc gccgtcaccc ctgtccctcc gctgcagcca gcatagacga acgacaccgc
     2101 acacggcaga cttgctgcac aacatagatc aaaggagacg tcatgttcgg tcgcttgctc
     2161 gccgtcgtcg cattcgcagt gatcgcgctt actcccgcgc atgcccagac ctggccggcg
     2221 aaggcggtac gcatcgtcgt gcccttccca cccggtggca cgaccgacat cgtggcgcgc
     2281 tcgctcggcg tcgaactgca gcgcatgtgg cagcacgcgg tcgtcatcga gaatcgcccg
     2341 ggcgccggcg gcaacatcgg cgccgacgcg gtggccaaaa gcgctcccga tggctacacg
     2401 ctcctgatgg gcaccgtcgg cacgcatgcg atcaatgcgg cgctgtttgc gcagagcgga
     2461 gcgaagatgc cgttcgatcc ggcgaaggac ttcgttccga tcacgctcgc cgcgggcgtg
     2521 cccaacgtga tggtgatcaa ctccaagctg ccggtgaaca cggttgccga attcatcgag
     2581 tatgcaaagg cgcggccagg acagttgaac atggcctcga gcggcaatgg cacgtcgatc
     2641 cacctgtcag gcgaactctt caagacggtc accggcatct acatggtgca ctttccgtat
     2701 cgcggctcgg cgccggccgt caccgacctg atcgccggca acatgagcgt gatgttcgac
     2761 aatctgccgt cggagctgcc gcacatcaag agtggccggc tgaaggcgct ggcggtcact
     2821 tcgcgcacac gttcgccggc cctgccgaac gtaccgacga ttgaagaggc ggccgggctg
     2881 aagggctttg acgccagttc ctggttcggg ctgttcgcac ctaccggcac gccccgcgcc
     2941 atcgtcgaca aaatccaggc cgatgtcgcg aaggccctgg cgctgcccga agtgcgcgag
     3001 cgattcctag cccagggcgc tgaccccggc ggcagcaccc ccgaacagtt cgccgcgttc
     3061 atccgcgccg agaccgacaa gtggacgcgg gtcgtgaagt tctccaacgc caaggtcgat
     3121 tgagcgcagc gccgggccgc cccaagtcag ctcgcgccgc agcgcgccgc gcgcagcgcg
     3181 gaggctccat gagagccggc gggaccatcg cgacttcctt cggtgtgatc gaatccgcga
     3241 ccgcgtatcg ggaacgtgtc gcggcgacaa tcgttgcgat gttgcgcgac gatccggcca
     3301 tcgccgcgat cggcgaaggt ggcgcggcgg cccgtagccg tgccgacgag ttctccgatc
     3361 tcgacctgat gatcgtggcg ccggtggctg gcgccgcggc gatcttcgag atcgtcgagg
     3421 cagcgttgcg gaccatcgcg ccgatcacgc acacctggcg cgtcgagcca cccggatttc
     3481 ccgacatggt ccagcggttc tacttcctcg ctgacgcgcc tcgccacttt gcggtcgact
     3541 gctcggtgat caccccggcc ggcatcgccg cgttcctcga acgggaacgg catggcgaga
     3601 tgatcgtctg gttcgaccga gatgccacgc tgacatcgcg cccgatcgac ggagaaacac
     3661 tggcgcgacg gcgtgcgaat cgactcgcgc agttgcgcgg ctcggtgccg gtctattcaa
     3721 tgctggtgga caaggaactg gagcgcggac acgcgctcga ggcgtatggc ttctatcagg
     3781 tgctgctgcg tgcactgatc gaactgcttg gcatgcggca ccggcccgac cgattcgact
     3841 atagctggcg ctatgtcgag cgcgaactgc cgcggtcggc gcaggcactg gtcgcacgac
     3901 acgcgttcgt gcgagacggc aagtcactgg cgctactcag cgcatcgctg gtcgacgaaa
     3961 tcacaggact gctggacaca ctgaccgcgg ccgaggctgc agccgacgct gcggctcccg
     4021 tcaacgcggc tgcgccaggc gatcgatcat cgccataatc gccttgacct gcacaccggt
     4081 cttcaccgcg tagtcggtgc ccgtgtagcc ccaccagtcc cagcagccgt tcggattgaa
     4141 tggcagccac cacgtaaaca tcgtgggctg gctcgcctgc gcctgcggat aaagcacgat
     4201 caggtcatgc gccgccgccc aggcgttata gccggcatcg cgcacgaaaa cttcgccgac
     4261 agcggtcacg ccctgatggc aaccgtgcaa cgccacatgc acgcggcact tcgtgttctt
     4321 ctcgcactgc gacggcacga aggcgtagcc ggtcgcgccg agcgacgaca tcgcccacca
     4381 gcgtcgccat gttggaacga attccgcctg gccgaagacc acgatgcgcc cctgcgatgg
     4441 acctgggccg gggcctgggc ttttgctcgc acctggatgc aatgacgtca acatctcgcc
     4501 gacgagatcg acgccgcagc ggttgatgaa ggggctcgcg ctggcatcgc atgcgacgcc
     4561 ctgcgtcggg gtcggcaggc catgtcccag cccgggctgc acccggaact gcgccccgga
     4621 gacgttgtag tgggcgaaga attcgtagtt cgcgcgcacc accggggtca ccacccggct
     4681 gtcggcttcg ccggccagga gccaggcgcg catgccgcgc aggttctgcg tcgaatcgat
     4741 cagtcgcagc gtcgccagcc gttcggcatc cttgatcgag ctgcctgcac ccggactgcc
     4801 ctgcatgcaa atgctttccg cacgggtcgg attgatgccg acgcagtagt acggcccacc
     4861 ggcaaagaca cccactccgt tgacacgagc cgaatgcgcg acggcaagct gcacggccat
     4921 gaacgctccg gaagacagcc cggaaaccgt gatgcggtcg ctgtgcgcgt cgaacgccgg
     4981 caaccgctgt gccgcggcgg cgatagttat ccacgctgcc gctgcagcga taatcaggaa
     5041 ccgcccaccg caccaccaca tgatgacctc cgaacgccgc acgccgctgc tcgccgcact
     5101 gctcatagtc acgatcatcg cactcgccat gtccggctgg cggccgtacg accgaatcac
     5161 ctggttcatg gaagtcgcgc cggtgctgat cgccatcccg ctgatgctgg ccacgcgcga
     5221 acgctatccg ctgaccacgt tgctgtacgt gctgatctgc gctcacgcgc tggtactcat
     5281 cctgggaggt gcctacacgt acgcgagggt cacgctcggg aactgggtac aggaagcctt
     5341 cgggctgtca cgcaatccgt acgacaagct cggtcacttc atgcagggtt tcgtaccggc
     5401 catgatcgcc cgcgagatcc tcgtgcgcgg caggtttgtg catggacgcc gcatgctggc
     5461 cttcctatgc gtgtgcgtcg cgctcgccat cagtgcagcc tacgagctgg tcgagtggtg
     5521 ggccgccctg gcgatgggcc agggtgccga cgagtttctc ggcacgcagg gtgatccatg
     5581 ggacacccag tccgacatgt tcctggccat ggtcggtgcg gtcactgcac tggtgaccct
     5641 gtccgagctc cacgatcgcc aacttgccgc cattgcaccg cagcagtcaa ggacttgagg
     5701 ctgacttgag gctgacttga ggctgacttg aggccaccgt cgcgtcgaat agtcggcacg
     5761 cgacttgcct aatcctccat acggtggatt gccgatgtgg cacaacaatc ccactgtcac
     5821 attgcgcgcg gaaaatgcgc tgcggaggcg gtcatgctga tgcgggtaag agagcggaac
     5881 aacgagatcg agatccagtt cagtggtctc ggcggccgcc agcatgcggt cctcaccgcc
     5941 ttcagtggct cggcggacgg cgacgaacga ctgttcgacc gcgccaaact cgagtccctg
     6001 aaagtgcgtg cgcgcgccga tgcgatgaac atccgactgc gcgcaaagtc cggcgagtcc
     6061 ttcgacgtgt cacatcttta ccgcagcctg cgtcgcgcgc tggtcgaggg cgagcgcact
     6121 gcattcggca gcctggccgg ctgaccgcgc gaggcgattc agttcagaag cgcaggccga
     6181 cgccgagcga catcatcggc gcgaagcgcg cctcgtccag acccgaaggg gcgtccagcg
     6241 cgcgcgcatc cggcccgcag agcgctgtcg gaatgcccgc gggacagccc caggtggaca
     6301 gattgctgcg ctgatacatc accccgagat cggcgctgaa atacaggccg ctgcgtgaac
     6361 cggcacccag accccagccc aggccgagat acggattgcc gcgcgccagc catgaacggg
     6421 gatccagacc cgcggcactg agccccggat cgcgcaggta ccgctccgac ccgaaccccg
     6481 aggtcgccaa gggctcgagc cgcagtgagc cgtaggccag tccgcctgac aggcgaaatc
     6541 cgttggacaa aggatgccag tcggcgagaa ccatggaact cgcgacaaga ccgtcgctgg
     6601 ccagcagtcc gggtcggccg ccgatccagc cgcgcagtcc gatgcccgga ctcagttcga
     6661 ccgcgtgctc cgcttcgatc gggaccctga gcccgggtcc ctgcagctgc gccagggccg
     6721 agccggttgt cagcatcaat gccagtccac cgatccgcca gtgactcatg cccacctccg
     6781 caacaccgct gcagccccca cgaaaaaggt aggccaattc gaacctgtcg caaacccgaa
     6841 gattcacgca aatgcggcgt caattcactg cagccgcatg atcacgggat agaggcttgt
     6901 cctgccgcaa cggagtgaac actcgcgcgt cgcgaaattg cgacgttact cctcaaagcg
     6961 cggcttgcgg ggccctggcg ggcgatcacc acgctcggtg cggcgcgggg gtcccgacga
     7021 acgttcacga aaggccggcg ggcgatcgcg cggcggaccg gagggacggc cgctcgcgaa
     7081 gcgtgccagg cgcatcacca agcgaagcgc ttcattgacc gattcggaat cacgaaagac
     7141 ccgcgcgaca tccggatcga gccggatcgc gcccgacacc ggagcatgca tccgctcgcg
     7201 gggtgcctcg ccgcgagccg gccgctcggc ccgcttgaac gggtcgccga acgtgcgcgc
     7261 ctgcgcagcg cgtgcgcgcg ggccggtgcg cgtgcccgtg ggcgggccgc ggcggtcttt
     7321 tttttctcgt tctttcatcg gtgcctcctc aagcaggggt gattcaaaag tttccagcgc
     7381 gcatgaagca cgctgtcagg
//
LOCUS       DATIRT010000073         5410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1483196, whole genome shotgun sequence.
ACCESSION   DATIRT010000073 DATIRT010000000
VERSION     DATIRT010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1483196"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VLN25_01825"
     CDS             <1..462
                     /locus_tag="VLN25_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSQ09343.1"
                     /translation="DEERPKREPVAPGSYTSGVVILLSDGRRTTGPDPIQIAKLAADR
                     GVRVYTVGFGTLQGTTIGFEDYQIYVRLDEETLKAIAKVTGGEYFHAGTAADLRTVYQ
                     NLNAKLEMERQQTEVGALFSGIAALFGLFAALLSVWWFYRAPGSRRTATPR"
     gene            complement(459..1352)
                     /locus_tag="VLN25_01830"
     CDS             complement(459..1352)
                     /locus_tag="VLN25_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HSQ09344.1"
                     /translation="MAGEFRVGVSGWRYAPWRGAFYPETLRQADELSYLASRLATVEI
                     NGTFYSLQSPTSFLSWYAATPDGFIFSVKGPRYITHILRLKDFERPLANFLASGLFNL
                     GDKLGPLLWQFPPNLRFNADRFEAFMASLPHDTSAALKLARRRDARMTGRARLAIDTP
                     RPMLHAIEIRNDTFLDARFIALLRRYRIALVIADTGGKWPSPMDVTADFLYLRLHGAT
                     ELYKSRYSEAMLRQWAGRIGAWRAGGQPVDVTRVVDAAPSRQSRNVYCYFDNTDKLHA
                     PANARRLLELVNGSPAKRRAA"
     gene            1530..2510
                     /locus_tag="VLN25_01835"
     CDS             1530..2510
                     /locus_tag="VLN25_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HSQ09345.1"
                     /translation="MKRWLFGFALSLFAAAAMTQTIVDLDYVKQALGRGALFWDARDE
                     DAYAKGHIPGAVNIGDPTLTLRDANTEDYLPLADLEKALGVAGIDPTREVIVYGDRGL
                     PSAYFAQLTLRYFGAGNARVFHEGIEGWRAGGQPLQTAAVKGKPVALKLLPQPGVLVS
                     TKEVVAALKRPDVQFLDVRTAKEFKAEDIRAIRGGHIPAAVNVPFEQNWVDPETRSKL
                     RAGKIKDSSGMALKPRAELAKLYETLDPAREIIVYCQSGNRASESAAVLADLGYKNVK
                     VYDSSWLGYSSWLSAPAEDEVWTNFGSLPAAVKGLENRFGEMERAAKPRQ"
     gene            2674..3390
                     /gene="aqpZ"
                     /locus_tag="VLN25_01840"
     CDS             2674..3390
                     /gene="aqpZ"
                     /locus_tag="VLN25_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HSQ09346.1"
                     /translation="MTPKLAAEFLGTFWLVLGGCGSAVLAAAFPELGIGFVGVSLAFG
                     LTVLTGAYALGPISGGHFNPAVSVGLWAGGRFPATQLLPYIVVQVMGAIVAAGVLYLI
                     ASGKAGFDVTAGFASNGYGEHSPGKYRFGASAITEIVLTFMFLIVILGATHKRAPVGF
                     AGLAIGLALTLIHLISIPVTNTSVNPARSTGPALFVGGWAVEQLWLFWVAPVIGALLA
                     GWFSRVIFDSAEAPPVVGRR"
     gene            complement(3502..4452)
                     /locus_tag="VLN25_01845"
     CDS             complement(3502..4452)
                     /locus_tag="VLN25_01845"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HSQ09347.1"
                     /translation="MLDLRLLHQALTLARHRNFARAADALHVTQPALSRSIAGLETSL
                     GEKLFNRTQQGVEPTAFGQMLLGRAQALLDGASELERDFKLMRGLDIGELRVGAGAYP
                     AEISLGAAVAQLARRHPALRIELSITDLRTIVESILARKLDLAVLELSTVEAETRVAV
                     EALPQHAARFFVRHDHPLAGARNLTLEQVLSFPFVGTRMPKRLAPRFLQAAKAGGIDP
                     DTGDYLPPVKVDSLRVAKEVVLGSDSVGVAPLPLIRRDVDDGALLALPLQLPWMTTGY
                     GFVYLRDRMLPPAAEAFMAEVRAVERRLVETHGLNDRQIG"
     gene            4614..>5410
                     /locus_tag="VLN25_01850"
     CDS             4614..>5410
                     /locus_tag="VLN25_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HSQ09348.1"
                     /translation="MAFNYSPVVRAASVLLVVLLAAPAALAQPKKPNILVLWGDDIGQ
                     FNISAYNMGMMGYKTPNIDSIAKQGAIFTDWYGQQSCTAGRAAFITGQSPIRTGLTKV
                     GLPGAPEGMKKEDPTIATLLKAQGYVTGQFGKNHLGDRDDMLPTAHGFDEFFGNLYHL
                     NAEEEPENPDYPKSAEFRKKFGPRGVIHSFAGGKITDTGPLTRKRMETIDYEVTEKTL
                     DFMERAKKADKPFFIWWNSTRMHIVTHLRPEVKGKTGLGVYADGMVE"
BASE COUNT          968 a         1767 c         1766 g          909 t
ORIGIN      
        1 gatgaagaac gcccgaagcg cgagccggtg gcgcccggct cttacacctc gggcgtggtc
       61 atcctgctct cggacggccg ccgcacgacc ggcccggacc cgatccagat cgcgaagctg
      121 gcagccgacc gcggcgtgcg cgtctacacg gtcggcttcg gcacgctgca gggcacgacc
      181 atcggtttcg aggactatca gatatacgtg cgcctcgacg aagagaccct gaaggccatt
      241 gccaaggtca ccggcggcga gtacttccac gccggcaccg ccgccgacct gcgcaccgtc
      301 taccagaacc tgaacgccaa gctggagatg gaacggcagc agaccgaggt cggtgcactg
      361 ttcagcggca tcgcggcact gttcggtctc ttcgcagcgc tgctctcggt gtggtggttc
      421 taccgcgcac ccggatccag aagaaccgcc acgccgcgct aggcagcgcg tcgcttggcc
      481 gggctgccgt tgacgagttc gagcagacgc cgggcgttgg cgggtgcatg cagcttgtcg
      541 gtgttgtcga agtaacaata gacgttccgg gattgccgcg acggagctgc gtcgacgacg
      601 cgcgtgacgt cgacgggctg gccgcccgcg cgccatgcac cgatccggcc cgcccactgc
      661 ctaagcatcg cttcgctgta tcggctcttg tatagctcgg tcgcgccatg cagccgcaga
      721 tagaggaagt cggccgtcac gtccatcggc gacggccact tgccgcctgt atcggcaatc
      781 accagcgcaa tccgatagcg gcgcagcaat gcgatgaagc gcgcatcgag gaaggtgtcg
      841 ttgcggatct cgatcgcgtg gagcatcggg cgcggtgtgt cgatggcgag ccgcgcgcgg
      901 ccggtcatac gcgcatcgcg ccggcgcgcc agcttcaagg cggccgatgt gtcgtgcggc
      961 aacgaggcca tgaaagcttc gaatcggtcg gcattaaagc gcaggttggg cggaaactgc
     1021 catagcagcg gaccgagctt gtcgccgagg ttgaacagtc ccgacgcgag gaagttggcc
     1081 agcggacgct cgaaatcctt caggcgcagg atgtgcgtga tatagcgcgg gcccttgacc
     1141 gagaagataa agccgtccgg cgtcgccgcg taccacgaca aaaagctcgt tggcgactgc
     1201 aaggagtaga aggtgccgtt gatctcgacc gtcgccagcc ggctcgccag gtacgacagt
     1261 tcgtccgcct gccgcagcgt ttccggatag aacgcaccgc gccacggcgc atagcgccac
     1321 cccgaaacgc cgacacgaaa ctcgcccgcc atgccgtttc cttagcaatc ggcctgcccg
     1381 cggacggcgc cgctggcgca gcctcacggc agcttcgcag ggggatcttg cgcctgatca
     1441 gcacccggtc gagcagcgct tatatactcc ccccggtatg taatcgattt gccggtcatg
     1501 cggggccggc gttgcccggg gggatatcaa tgaaacgttg gctgttcgga tttgccctgt
     1561 cgctgttcgc cgctgcggcg atgacgcaga cgattgtcga tctggactac gtaaagcagg
     1621 cgctgggccg cggtgcgctc ttctgggacg cccgcgatga ggacgcctac gccaagggcc
     1681 acatcccggg agccgtcaat atcggcgacc cgacactgac gctgcgcgat gcgaacaccg
     1741 aggactacct gccgctggcc gacctggaga aggcgctggg ggttgcgggc atcgatccga
     1801 cccgcgaggt catcgtctac ggcgaccgcg gcctgccgtc ggcgtacttc gcgcaactca
     1861 cgctgcgata cttcggcgcg ggtaacgccc gcgtcttcca cgaaggcatc gaaggctggc
     1921 gcgctggcgg ccagcccttg cagacggctg ccgtgaaggg caagccggtc gcgctgaagc
     1981 ttttgccgca gccgggcgtg ctcgtcagca cgaaggaagt cgtcgccgcg ctcaagcgcc
     2041 cggacgtgca gttcctcgat gttcgcacgg cgaaggagtt caaagccgag gacatccgtg
     2101 cgatccgcgg tggccacatc cccgcagccg tcaatgtgcc gttcgagcag aactgggtcg
     2161 atccggaaac gcgctccaag ctgcgtgccg gcaagatcaa ggactcgagt ggaatggcgc
     2221 tgaagccgcg cgcggagctc gccaagctct acgagacgct tgatcctgcg cgggagatca
     2281 ttgtctactg ccaaagcggc aatcgcgcct cggagagcgc cgccgtgctg gccgatctcg
     2341 ggtacaagaa cgtcaaggtg tacgactcgt cctggctcgg ctattcgagc tggctcagtg
     2401 cgccggccga agacgaggtc tggaccaact tcggttcatt gccggccgcg gtcaaggggc
     2461 tcgagaaccg ctttggtgag atggagcggg cggccaagcc acgccagtaa cggcatgcga
     2521 tccccggcgt caagtcacac gaactttcgc gacccctttt ttggtgcgct cctttgccac
     2581 aggggtcggc ctgcgcgccc cacgcagtcg acggtgaggt agtctgcgcg cctcgttgat
     2641 gccgaacatc gaaaacgaaa ggaaaaacga acgatgacac ccaaactcgc agcagaattt
     2701 cttggcacct tctggctcgt gctcgggggc tgcggcagcg cagtgcttgc cgctgctttt
     2761 cccgagctcg gcattggttt tgtgggtgtg tcgctggcgt tcggcctgac ggtactgacc
     2821 ggcgcctatg cactcggtcc gatctccggc ggccacttca accctgcggt gtccgtcggc
     2881 ctgtgggcgg gcgggcgctt ccccgcaacg cagttgctgc cgtacatcgt cgttcaggtt
     2941 atgggcgcca tcgtcgccgc aggcgtgctc tacctgatcg cgagcggtaa ggccggtttc
     3001 gatgtgacgg ccggctttgc ctccaatggt tacggcgagc actcgccggg caagtaccgt
     3061 tttggcgcgt ccgcgattac cgaaatcgtc ctgaccttca tgttcctcat cgtgatcctc
     3121 ggcgccacac acaagcgcgc gcctgtgggc tttgcgggtc ttgcgatcgg actcgcgctg
     3181 acactcatcc acctgatcag cattccggtc accaacacgt cggtgaaccc ggcgcgcagc
     3241 accggaccgg cgctgttcgt cggcggctgg gcggtcgagc aactatggct cttctgggtc
     3301 gcaccggtca tcggggcgct gttggccgga tggttttcgc gggtgatctt cgactcggcc
     3361 gaggcaccac cggttgtcgg gcggcgctaa ggtcagtgcc acagggcccg tatcgacagt
     3421 cgcaggcccc ggttctgcgc cttgtattcc gccgacggca ccgttgaacg atgcagaaac
     3481 cggactgacg gtaagggcag gtcacccgat ctgacgatcg ttcaggccgt gcgtctccac
     3541 aagccggcgt tcaaccgcac gcacctcggc cataaacgct tcggccgccg gtggcagcat
     3601 gcggtcgcgc aggtagacga agccgtaacc ggtcgtcatc cacggcagtt gcagaggtaa
     3661 cgcgagcagt gcgccgtcgt ccacatcccg gcggatcagc ggcagcgggg cgacaccgac
     3721 gctgtcgctg ccgagcacca cttccttcgc gacgcgcagc gaatccacct tgaccggcgg
     3781 aaggtaatcg ccggtgtccg gatcgatgcc gccggctttc gccgcctgga ggaagcgcgg
     3841 cgccagccgc ttcggcatgc gcgttccgac gaagggaaac gacagcacct gctccagcgt
     3901 caggttgcgc gcccccgcca gcgggtgatc gtgacggacg aagaagcgag ctgcatgctg
     3961 cggcagcgct tcgaccgcga cgcgggtctc ggcttcaacc gtggagagtt ccaggaccgc
     4021 caggtcgagc ttgcgcgcaa ggatcgattc gacgatcgtc cgcaggtccg taatcgacaa
     4081 ctcaatacgc agcgccgggt ggcgccgcgc cagctgcgcg accgccgcgc cgagcgatat
     4141 ttcggcgggg taggcgcccg cgccgacgcg tagctcgccg atatccagcc cgcgcatcag
     4201 cttgaagtct cgctcgagct cgctggcacc gtcgagcagc gcctgggctc gtcccagcag
     4261 catctggccg aaagcagtgg gttcgacgcc ctgctgcgtc cggttgaaga gcttctctcc
     4321 gagtgatgtc tcgaggccgg caatgctgcg cgacagcgcc ggctgcgtca catgcaacgc
     4381 atcggccgcc cgcgcgaagt tgcggtgccg cgcaagcgtt agcgcctggt ggagaagccg
     4441 gaggtcgagc atatgcgtcg acgttatcag acgatgccta agcggcattg gaccgggcgc
     4501 cctcgtgccc ttaccttcgc gcctcgcgcg ccggaggtgg actggatgaa agagcagccg
     4561 gcgccagcgc aggctcggtt ccccgcgggc gcgccacctt tcaggagaga accatggcct
     4621 tcaactattc gccggttgtc agggcggcat cggtgctgct ggttgtgctg ttagcggcgc
     4681 ccgccgccct cgcccagccg aagaaaccca acatcctcgt cctctggggt gacgacatcg
     4741 gccagttcaa catcagcgcc tacaacatgg gcatgatggg ttacaagacg cccaacatcg
     4801 acagcatcgc gaagcagggc gcaatcttca ccgactggta cggccagcag agctgcaccg
     4861 cgggccgcgc cgccttcatc accggccagt cgccgatccg caccggtctc accaaggtcg
     4921 gcctgcccgg cgcgcccgag ggcatgaaga aggaagaccc gaccatcgcc acgctgctga
     4981 aggcgcaggg ctacgtcacc ggccagttcg gcaagaacca tctgggcgac cgtgacgaca
     5041 tgctgccgac cgcgcatggc ttcgacgagt tctttggcaa cctttatcac ctgaatgccg
     5101 aagaggagcc ggagaacccc gactacccga aaagcgcgga gttcaggaag aagttcggcc
     5161 cgcgcggcgt catccacagc tttgccggcg gcaagatcac cgacaccggc ccgctgaccc
     5221 ggaagcgcat ggaaacgatc gactacgaag tcaccgagaa gacgctggac ttcatggagc
     5281 gcgcgaagaa ggccgacaag ccgttcttca tctggtggaa ctccacgcgc atgcacatcg
     5341 tcacgcacct gcggccggaa gttaaaggga agacgggctt gggagtctac gccgacggca
     5401 tggtcgagca
//
LOCUS       DATIRT010000074         3087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1778381, whole genome shotgun sequence.
ACCESSION   DATIRT010000074 DATIRT010000000
VERSION     DATIRT010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1778381"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..977)
                     /locus_tag="VLN25_01855"
     CDS             complement(90..977)
                     /locus_tag="VLN25_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSQ09349.1"
                     /translation="MTELFGIPLSALLSQLLLGLVNGSFYAMLSLGLAVIFGLLNVIN
                     FAHGALYMMGAVFAWMGLTYFGINYWVMLIVAPLIVGLFGVIIERLLLRHLYKLDHLY
                     GLLLTFGLTLLIEGFFRSFYGVSGLPYQVPEVLEGATNLGFMIMPNYRGWVVLASLVV
                     CLGTWYVIERTKLGALLRAGTENAKLTEAFGVNVPLMVTLTYGFGVALAGFAGVLAAP
                     ILQVQPLMGSNLIIVVFAVVVIGGMGSIMCSILTGLGLGVIEGFTRVFYPEASSTVVF
                     VIMEIVLLIRPAGLFGKEK"
     gene            complement(1031..2245)
                     /locus_tag="VLN25_01860"
     CDS             complement(1031..2245)
                     /locus_tag="VLN25_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSQ09350.1"
                     /translation="MKLKKIAAAVAACGLALGFSGAQAQKLSGDKIVIGFITDMSSVY
                     SDIDGAGGVEAIRMAIADMQKEFPGKKIEVVFADHQNKADISSSKVREWIDQSGADVI
                     IGGTNSAAQLAAAKVATEKKRPIIVVGAATSRLTNEDCSPYIVHYAYDTIALANGTGG
                     AVVKSGGKSWFFLTADYAFGHSLEKDTGEVVKAAGGTVVGAVRAPLNASDFSSFLLQA
                     QNSKAQILGLANAGGDTINSIKAANEFGVTKTMKLAGLLMFINDIHSLGLKTTQGMYL
                     TDAWYWNQTPETRAWGRRFFEKMKRMPSFLQAGDYSAVSQYLLAVKAAGTDDADAIMA
                     QLKKQKVNDMFAKGGVIRADGRMVHDMYLMQVKSPDQSKEPWDYFNVVQTIPGDQAYQ
                     SLAQSRCSLIKK"
     gene            complement(2277..2981)
                     /locus_tag="VLN25_01865"
     CDS             complement(2277..2981)
                     /locus_tag="VLN25_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSQ09351.1"
                     /translation="MADALEIRELHAWYGESHILHGVDLRVARGEVVTLLGRNGAGRS
                     TTLRAILGLTGTRKGSIQVNGTETVGMATHRVAHLGIGYCPEERGIFSSLSCEENLML
                     PPRVDASGGMSVEEIYAMFPNLKERARSQGSRLSGGEQQMLAVARILRTGAHLLLLDE
                     ISEGLAPVIVKKLGEMIRALKARGYTIVMVEQNFRFAAPLADRFYVMEHGRIVETFAA
                     AELKAKEETLHQLLGV"
     gene            complement(2983..>3087)
                     /locus_tag="VLN25_01870"
     CDS             complement(2983..>3087)
                     /locus_tag="VLN25_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSQ09352.1"
                     /translation="SVLAEGPYETVSRNAAVMEAYMGTSAAELEGIGQ"
BASE COUNT          582 a         1033 c          906 g          566 t
ORIGIN      
        1 cgaagaccgg gtacgcgccg accaggggaa ggatggccac cagcgccacg agcgccagat
       61 acaccacaag ctctgtcgag gccttcactc tacttctcct tgccgaagag gccggcgggc
      121 cggatcagca gcacgatctc catgatgacg aacaccacgg tcgacgacgc ctccggatag
      181 aacacgcgtg tgaagccctc gatcactccg agcccgaggc cggtcaggat cgagcacatg
      241 atcgagccca tgccgccaat caccaccacg gcgaacacga cgatgatcag gttgctgccc
      301 atcagcggct gcacctgcag gatcggtgcg gcgagcacgc ccgcgaaccc tgccagcgcc
      361 acaccgaagc cgtacgtcag cgtgaccatc aacggcacgt ttacgccgaa cgcctcggtc
      421 agtttggcgt tctcggtccc cgcgcgcagc agagccccga gtttggtgcg ctcgatgaca
      481 taccaggtcc cgaggcagac gacgagtgac gcaagcacca cccatccccg gtagttgggc
      541 atgatcatga agccgaggtt ggttgcgccc tccagcacct cgggcacctg gtacggcagg
      601 ccggacactc cgtagaagga acggaagaac ccttcgatca gcagcgtcag gccgaaggtc
      661 agcaacaggc cgtaaaggtg gtcaagcttg tataaatgcc gaagcagcag gcgctcgatg
      721 atgaccccga acaggcccac gatcagcggc gcaacgatca gcatcaccca gtagttgatg
      781 ccgaagtacg tgagtcccat ccacgcgaac accgcgccca tcatgtagag cgcgccgtgc
      841 gcgaagttga tgacgttcag cagcccgaag atcaccgcca gtccgagcga aagcatcgcg
      901 tagaacgagc cgttgaccag tcccaggagc agctgggaca gcagtgcgga cagcggtatg
      961 ccgaacagtt cagtcatctt gaatgcaagg ttgtcaccgg gcgcctcggc gcccggtgcc
     1021 cgtcgacgtc ttacttcttg atcagcgagc agcgcgactg tgccagggac tggtaagcct
     1081 ggtcaccggg aatcgtctgg accacattga agtagtccca cggctccttc gactggtcag
     1141 gcgatttcac ctgcatcagg tacatgtcgt gcaccatgcg gccgtcggcg cggatcacgc
     1201 cacccttggc gaacatgtcg ttgaccttct gcttcttcag ctgcgccatg atcgcatcag
     1261 cgtcatccgt gccggcggcc ttgacggcca acaggtactg cgacaccgcg gagtagtccc
     1321 ccgcctgcag gaacgacggc atgcgcttca tcttctcaaa gaagcggcga ccccaggcgc
     1381 gggtttccgg cgtctggttc cagtaccagg catcggtcag gtacatgccc tgcgtcgtct
     1441 tcagacccag gctgtggatg tcgttgatga acatcagcag gcccgccagc ttcatcgtct
     1501 tggtcacgcc gaactcgttg gcggccttga tcgagttgat cgtgtcgccg cccgcgttcg
     1561 ccaggccaag aatctgcgcc ttgctgttct gcgcctgtaa caggaacgac gagaagtccg
     1621 atgcgttgag cggcgcgcgt actgcgccga cgacggtccc ccccgccgct ttgacgacct
     1681 cgccggtgtc cttctcgagc gagtggccga aggcgtagtc cgccgtcagg aagaaccagc
     1741 tcttgccgcc gcttttcacg accgccccgc cggtaccgtt ggccagcgcg atcgtgtcat
     1801 aggcgtagtg cacgatgtac ggtgagcagt cctcgttggt cagccgtgac gtggccgcgc
     1861 ccacgacgat gatcggacgc ttcttctcgg tcgcgacctt tgccgcagcc agttgcgctg
     1921 ccgaattggt gccaccgatg atcacgtcgg cgccggactg gtcgatccat tcccgcacct
     1981 tgctcgacga gatgtcggcc ttgttctggt gatcggcgaa gacgacttcg atcttcttgc
     2041 ccggaaattc cttctgcatg tcggcgatcg ccatgcggat cgcctcgaca ccgccggcgc
     2101 cgtcgatgtc ggaatagacc gacgacatgt cagtgatgaa cccgatcacg atcttgtcgc
     2161 ccgacagctt ctgggcctgc gcgccggaga aacccagcgc cagtccgcat gccgcaactg
     2221 ccgcggcgat tttcttcaac ttcattgaaa ttccctcctc aaaaggttga tcactgctag
     2281 acaccgagca actgatgcag cgtctcttcc ttggctttca gttccgcagc cgcaaaggtt
     2341 tcgactatgc gcccatgctc catgacataa aaccggtccg ccagcggtgc cgcaaaccgg
     2401 aaattctgtt cgaccatcac gatcgtgtat ccccgcgctt tcagcgcgcg gatcatctcg
     2461 ccgagcttct tgacgatgac gggggccagc ccctctgaaa tctcgtcgag cagcagcagg
     2521 tgggcgccgg tgcgcaggat acgggctacg gccagcatct gctgctctcc tccggacagg
     2581 cgcgagccct ggctcctggc gcgctccttc aggttgggaa acatcgcgta gatctcttcg
     2641 acgctcatgc caccgctggc atcgacccgc ggcggcagca tgaggttctc ctcgcacgac
     2701 agactggaga agatgccgcg ctcttccggg cagtagccga tgccgaggtg cgccacgcga
     2761 tgcgtcgcca tgccgaccgt ctcggtgcca ttgacctgaa tcgagccctt acgcgttccc
     2821 gtcagaccca ggatggcgcg cagcgtcgtc gaacgtccgg cgccgttgcg tcccagcagc
     2881 gtcaccactt caccgcgcgc cacgcgcagg tcgaccccgt gcaggatgtg ggactcgccg
     2941 taccaggcgt gcagttcccg gatctcgagc gcgtcggcca tcctactgcc ctatgccttc
     3001 cagttcagcg gcgctggtgc ccatgtaggc ttccatgacc gcagcgtttc tggagaccgt
     3061 ctcgtagggc ccctcggcca gcaccga
//
LOCUS       DATIRT010000075         9283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1680097, whole genome shotgun sequence.
ACCESSION   DATIRT010000075 DATIRT010000000
VERSION     DATIRT010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9283
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680097"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="VLN25_01875"
     CDS             complement(<1..804)
                     /locus_tag="VLN25_01875"
                     /inference="COORDINATES: protein motif:HMM:NF016999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HSQ09353.1"
                     /translation="MARVVVALVVALIGAGACIWIRAPLPWLIGPLVVVAMASMLNVR
                     LAAPPAARQLGQWAIGVALGLYFSPHVVREVVRLAPWLLLGIVFAVILGGLGAWLLRR
                     MTDTDETTAFFAMAIGGASEMATQAQRHGGQVERVAAAHSLRIMLVVLIVPLSFNLLQ
                     VHGLDPYVPLALSFDPVGFAVLVAVTLGAAVVMERFGSPNSWMIGPLLTAAAITATGH
                     IFSSLPVAVVNAGQLLIGISLGTHFTPEFFRAAPRYLAVVALITLAYLLS"
     gene            complement(864..2369)
                     /locus_tag="VLN25_01880"
     CDS             complement(864..2369)
                     /locus_tag="VLN25_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HSQ09354.1"
                     /translation="MTETLSALLTGFGVALAPNNLLWGFIGVTLGTAIGVLPGVGPAL
                     TVAMLLPLTVKLEPTGALIMFAGIYYGAMYGGSTTTILLNTPGESASIATALEGNKMA
                     KRGRAGPALATAAIGSFVAGTIATILLTLLAPLVVEVALKFGPTEYFALMVFAFVTVA
                     AVLGSSTVRGLTMLFLGVLLGLVGIDDQTGQMRFAFGVPELMDGVDVVVLAVGLFAVG
                     EALYVAAYQSRQHEVVEKMSGSIWMSREDWKRSWPAWLRATFIGFPFGTIPAGGAEIP
                     TFLSYALEKKLTKYPEEFGNGAIEGVAGPEAANNASSAGVLVPLLTLGIPTSATAAIL
                     LAAFQNYGIQPGPLLFQTQGALVWGLIASMYIGNILLLILNLPLIGLWVKVLMIPRPL
                     LYAGILVFATLGAYSLHQSVVDLATLYVFGLLGFMMRRWGFPVAPAVIGLILGPLAES
                     QFRRALAISQGDVSVFFTHPISASLLALTAALVLVPWIVRVVRERRGRAAA"
     gene            complement(2366..2878)
                     /locus_tag="VLN25_01885"
     CDS             complement(2366..2878)
                     /locus_tag="VLN25_01885"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HSQ09355.1"
                     /translation="MLPATHGGRVELILSLGVLALGIFATVVAFKLPEAGGYARIGPN
                     FMPKVVSAGLIVLGFWLLAEVFTGGWRDRVPDDPAERGEHAFHLGAFAWVSVGLFAQM
                     VLIHNAGFAIAAMVLYALVARGFGSDRWARDLTIGLVLGIAVHLFFVRFLNVNLPAGW
                     LQPLLGTAGL"
     gene            complement(2883..3845)
                     /locus_tag="VLN25_01890"
     CDS             complement(2883..3845)
                     /locus_tag="VLN25_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HSQ09356.1"
                     /translation="MNRREFTLVSIAAAAAAQWPMTAAAQAIANLKMMIPANPGGGWD
                     QTGRTLGAAMQSTKAVQSVQFDNKGGAAGTIGLAQFVNASKGDPNAMLIGGMVMVGGI
                     VLNKSPVDLNMVTPLARLTSEYEVVVVPANSPFKTMGDLVKAFKADPGKISWNGGSAG
                     GTDHILAGLIARAVGADPTKVNYIASKGGGDQIANIVGGHVSAGVAGLGEFAEHIKSG
                     RMRGLAVSGPAAIDGIPSLKEQGVDVVLGNWRGAFGAPGITPAQRDALIAAIKAGAES
                     AAWQETLKKMGWESVFISGDAFKTFLDEDTKRIRGILDSLGLAK"
     gene            complement(3940..5109)
                     /locus_tag="VLN25_01895"
     CDS             complement(3940..5109)
                     /locus_tag="VLN25_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSQ09357.1"
                     /translation="MKIPFSYVVRNLWVRKLTTALTAGGMALVVFVFAAVLMLDAGLK
                     STLVSTGSADNVVVIHQGSQSEVQSGLLRDQASLIETSPEVARGPDGRALVSKEVVVL
                     NSLPKLDEPTKRSNVVVRGLPETGKTLRPQVRMTQGRMFNAGSSEIIVGSSVARQFAG
                     VELGQHLSFAGRQWTVVGVFEAGKTAFDSEIWGDVEQMMQAFRRIAYSSVIARLADPN
                     SFDALKARLDDDPRLKVDIKRERRFYEDQSASLSNFITLLGMALSVIFSLGAMIGATI
                     TMYAAVATRTGEIGTLRALGFQRASILIAFLVESLLLALLGGLAGLIAASFLTAITVS
                     TTNFQSFSELAFSFTLTPRIVVQGLMFALLMGFVGGFLPAIRASRMKIVDALRAD"
     gene            complement(5446..6603)
                     /locus_tag="VLN25_01900"
     CDS             complement(5446..6603)
                     /locus_tag="VLN25_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011546674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HSQ09358.1"
                     /translation="MFFLKFVLRNVFRARLRSALTILGLVIAILAFGLLQTVVRAWYA
                     GADAASATRLISRNAISLTFAMPMYYREKIRAVPGVTGVSVSNWFGGIYKEPKNFFAQ
                     FAVDAPTYFPMYPEFIFQPEEYQAFLRDRRGAAIGRQLADLYGFKVGDTIPLKSGIYP
                     GNWEFTVRAIYEAKDETTITRQMYFHFDYLNEQIKKLFPRRADQAGVFIVGIADGQAA
                     ANISRDIDAEFKNSLAETLTETEKAFTLGFVAMIETIVVAIQGVSYVVILIIMAVAAN
                     TMAMTARERLPEYATLKALGFKPGTVAAMIMGEAMLLALIGTAIGMLLTTPVVRIFHA
                     ATVSVFAKMPLVADTFYLQAACALVVGVVAGIVPAIRAARVKIVDGLRHVA"
     gene            complement(6623..7342)
                     /locus_tag="VLN25_01905"
     CDS             complement(6623..7342)
                     /locus_tag="VLN25_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSQ09359.1"
                     /translation="MPHAEAGIDRRALVEIEHVSKSYVRGEQVVPVLNDINLVIREGD
                     FVAMMGPSGSGKSTLLNLVAGIDKPDAGRLAVAGEDITQLGDTELADWRARTVGFIFQ
                     FYNLMPVLTAFENVELPLQLTSLSRSERRERVELLLGMVGLTDRMKHYPNELSGGQQQ
                     RVAIARALVTDPLLVIADEPTGDLDRQSAADILGLLDRLNSELGKTIVMVTHDPRSAD
                     AAHHLIYLDKGQLTDTAPFGH"
     gene            complement(7382..8617)
                     /locus_tag="VLN25_01910"
     CDS             complement(7382..8617)
                     /locus_tag="VLN25_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSQ09360.1"
                     /translation="MADTPAAPTSLARLAIDRSAAPAQRRRRPLWKRWWVWLLVVVVL
                     LAVAWFARSRSAALPVEFATVAAAYPSTSISVLNATGRVVAQRRASVSSKGTGRLEWL
                     GVQEGQMVKEGDVIARLENRDVAAQRDQAAAQVQAARANLVQGEAELDDARSALKRSQ
                     DLARQNFISGSALDTAEARYNKTRAQIDTLKAQIAVAQANHRVTSVAYDQTLIRAPFT
                     GIVLTKSANVGDIVTPFSSASGTTGAVVTMADMETLEVEADVSESSIAKISVGQPAEI
                     QLDAFPELRLLGEVSRVVPTVDRSKATLLVKVKFVERDARVLPDMSAKISFLSRALTP
                     EERKPVPALRADAIVKRDGAEVVFVVDAGNTVKPAPVKAGGKIGDQVRIEGVAAGTRV
                     VANPPERLAAGALVEAVKK"
     gene            complement(8747..>9283)
                     /locus_tag="VLN25_01915"
     CDS             complement(8747..>9283)
                     /locus_tag="VLN25_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041491300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HSQ09361.1"
                     /translation="VRESTDEVETLGIARTLAEVERADVVLHLVDALRPSADEDVLAR
                     VTARAGRGVPLLTVVNKIDLTGALPHSEDAAVHLSARDGQGIDLLRERLYRLAGWDRT
                     ADGENVYLARERHLQALAQARIHLEAADGHAARRDAVLDLFAEELRLAQNQLASITGE
                     FSADDLLGVIFSRFCIGK"
BASE COUNT         1696 a         3182 c         2939 g         1466 t
ORIGIN      
        1 ggacaggaga taggcaagcg tgatcagtgc caccaccgca agataccgcg gcgcggcgcg
       61 gaaaaactca ggagtgaagt gcgtgcccag ggaaatgccg atcagcaact gcccggcatt
      121 gacgaccgcc actggcagtg acgaaaaaat gtgccccgtc gcggtgatcg cggcggcagt
      181 gagcagtggc ccgatcatcc agctgttcgg tgacccgaag cgttccatta cgaccgcagc
      241 gcccagcgtg actgcgacca gcaccgcgaa gccgaccggg tcgaagctaa gcgccagcgg
      301 gacatacgga tcgagcccat gcacctgcag cagattgaac gaaagcggca cgatcagcac
      361 gaccagcatg atgcgcaggc tgtgtgcggc cgccacgcgc tcgacctggc cgccgtgtcg
      421 ctgcgcctgc gtcgccattt cgctggcgcc gccgatggcc atcgcgaaga acgccgtcgt
      481 ctcgtcggta tccgtcatcc gccgcagcag ccatgcacca aggccgccga ggatgacggc
      541 gaagacaatc ccgagcagca gccatggtgc gagcctcacc acctcgcgca cgacgtgtgg
      601 tgagaagtac agcccgaggg ctacgccgat ggcccattga ccgagctgtc gcgccgccgg
      661 cggcgcggca aggcgcacgt ttagcatgct cgccatcgca actacgacca gcgggccgat
      721 cagccaaggg agcggtgcgc ggatccagat acatgctccg gcgccgatca gcgcgaccac
      781 gagcgcaaca acaactcgtg ccatcgtcgc gcgactgcga cccgactcgt ctggcgcgac
      841 tgccgctgcg ctttccggct cgctcacgcc gcggcgcggc ctcgccgttc gcgcacgacg
      901 cggacgatcc aaggcaccag caccagcgct gcggtcaacg ccagcagcga cgccgagatc
      961 ggatgcgtga agaacaccga cacatcgccc tggctgattg ccagcgcgcg acggaactgt
     1021 gactcggcca gcggcccgag gatgagaccg atcacggcgg gcgccaccgg aaagccccaa
     1081 cgccgcatca tgaaaccgag aagcccgaag acatacagcg tcgcgagatc gacaaccgac
     1141 tggtgcaggc tgtaggcgcc gagcgtggcg aacacgagga tgcccgcgta cagcagcggc
     1201 cgcggaatca tcagcacctt cacccacagc ccaatcagtg gcagattcag gatcagcaag
     1261 agaatgttgc cgatgtacat gctggcgatc aggccccaga ccagcgcacc ctgcgtctgg
     1321 aacagcagcg ggccgggctg aatgccgtag ttctggaacg ccgccagcaa tatcgccgcc
     1381 gtggcggagg tcggaatgcc cagcgtcagc aacggcacca gcactcctgc cgacgaggcg
     1441 ttgttggcgg cctcgggtcc ggcgacgcct tcgatggcgc cattgccgaa ttcctccgga
     1501 tacttcgtga gcttcttctc cagcgcatac gacaggaagg tcgggatctc cgcgccgccc
     1561 gccggaatgg ttccaaacgg gaagccaatg aaagtcgcac gcagccacgc cggccacgag
     1621 cgcttccagt cttcgcgcga catccagatc gagcccgaca tcttctcgac cacttcgtgc
     1681 tgccggctct ggtaggccgc cacatacagc gcttcgccga cggcgaagag gcccaccgcc
     1741 agtacgacca catcgacgcc gtccatcagt tcgggcacac cgaaggcaaa gcgcatttgg
     1801 cccgtctggt cgtcgatgcc caccagaccc agcagcacgc cgaggaacag catcgtcagg
     1861 ccgcgcacgg tcgaggagcc gaggaccgcg gccactgtaa cgaacgcgaa caccatcagc
     1921 gcgaagtact cggtcgggcc gaacttcagc gccacttcga ctaccagtgg cgccagcagc
     1981 gtcagcagga tcgtcgcgat cgttccggcg acgaacgagc cgatcgccgc ggtcgccagc
     2041 gccgggccgg cacggccgcg cttggccatc ttgttgcctt cgagcgcggt cgcgatcgac
     2101 gcggactcgc cgggcgtgtt gagcaggatc gtcgtcgtcg aaccgccgta catcgcaccg
     2161 tagtagatgc cggcgaacat gatcagcgcg ccggtcggtt cgagcttcac cgtcagcggc
     2221 agcagcatcg ccaccgtcag tgccggtccc acgcccggca gtacgccgat cgccgtgccc
     2281 agcgtgacac cgatgaaacc ccacagtaga ttgttcggcg ccagtgcgac gccgaaacct
     2341 gtcagaagtg cgctcagggt ttcggtcaca ggcccgccgt tcccagcagc ggctgcagcc
     2401 agccggcggg cagattgacg ttgaggaagc gcacgaagaa gagatgcacc gcaatgccga
     2461 gaaccagacc gatcgtcaag tcgcgcgccc agcggtcgct gccgaagccg cgcgcgacca
     2521 gcgcatagag caccattgcc gcgatcgcga agccagcgtt gtggatcagc accatctgcg
     2581 caaacagtcc cacgctgacc caggcaaagg cgcctaggtg gaaagcgtgt tcgccgcgct
     2641 cggccggatc gtcgggcacg cggtcgcgcc agccaccggt gaacacctcc gccagcagcc
     2701 agaacccgag cacgatcagc ccggccgaga caacctttgg catgaagttg ggcccgatgc
     2761 gggcgtaacc gcccgcctcc ggcagcttga atgcgacaac tgtcgcgaag ataccgagcg
     2821 ccagcacgcc cagcgacagg atcagttcga cacgaccgcc gtgcgtcgcc ggcagcatgg
     2881 gcttacttgg ccaggccgag ggaatcgagg atgccgcgga tgcgcttggt gtcttcgtcg
     2941 aggaaggtct tgaaggcgtc gccggaaatg aacaccgact cccagcccat cttctttagc
     3001 gtttcctgcc aggccgcaga ctcggccccg gccttgatcg ccgcgatcag cgcatcgcgt
     3061 tgagccggcg tgatgcccgg ggcaccgaag gcgccgcgcc agttgcccag cacgacatcg
     3121 acaccctgct ccttcaacga cgggatgccg tcgatcgccg ccggtccgga gaccgccagc
     3181 ccacgcatac gtccgctctt gatgtgttcc gcgaactcgc cgagaccggc cacgccggcc
     3241 gacacgtggc cgccgacgat attggcgatc tggtcgccgc cgcccttgga ggcgatgtag
     3301 ttgaccttgg tcgggtccgc gccgaccgcg cgcgcgatca gccccgccag aatgtggtcg
     3361 gtgccgccgg cggagccgcc gttccaggag atcttgcccg gatccgcctt gaacgccttc
     3421 acgaggtcgc ccattgtctt gaacggcgag ttcgccggaa cgacaaccac ctcgtattcg
     3481 ctggtgaggc gcgcaagcgg cgtcaccatg ttgaggtcga ccggcgactt gttcagcacg
     3541 atgccgccca ccatgaccat gccgccgatc aacatggcgt tgggatcgcc cttggacgcg
     3601 ttgacgaact gggcgagccc gatcgtgccg gccgcaccgc ctttgttgtc aaactgcact
     3661 gactgcaccg ccttggtcga ttgcatcgct gcccccagcg ttcgaccggt ctggtcccag
     3721 ccaccgcccg ggttggcggg aatcatcatt ttcaggttcg caatcgcctg cgcggcagcg
     3781 gtcatgggcc actgagcggc cgcagcggcg gcgatggaca cgagagtgaa ttcacgacgg
     3841 ttcatgacgt ctccttggct gctcttgtta tcggggaaac gcagccttgg cagcataccg
     3901 aaggccgggc ctctccgaca tccgcgctac ctgccgacct caatctgcgc gcagcgcatc
     3961 cacgatcttc atgcggctgg cgcgaattgc cgggaggaag ccgccgacga atcccatcag
     4021 cagcgcgaac atcaacccct gcacgacgat cctcggcgtc agcgtgaacg agaacgccaa
     4081 ctccgaaaac gactggaagt tggttgtcga caccgtgatc gcggtcagga aactcgccgc
     4141 gatcagccca gccagtccgc ccagcagtgc gagcagcagt gattcgacca ggaaggcaat
     4201 aagtatcgag gcacgctgga accccagtgc acgcagcgtg ccgatctcgc cggtgcgggt
     4261 tgcgacggcc gcgtacatcg tgatcgtcgc accgatcatc gcgccgagcg agaagatgac
     4321 cgacagcgcc atgccgagca gcgtgatgaa gttcgacagg ctcgccgact ggtcctcgta
     4381 gaagcgccgc tcgcgcttga tgtccacctt gagtcgcgga tcgtcgtcca gccgggcctt
     4441 cagcgcgtcg aaggagttcg gatcggccag cctggcaatg acggacgagt acgcaatacg
     4501 ccggaacgcc tgcatcatct gctcgacgtc accccagatc tcggagtcga acgccgtctt
     4561 gccggcctcg aacacaccga cgaccgtcca ctggcgcccc gcgaaggaaa ggtgctgccc
     4621 caattcaacg ccggcgaact ggcgcgcgac ggaactgccg acgatgatct ccgacgagcc
     4681 cgcgttgaac attcggccct gggtcattct cacctgcgga cgcagcgtct tgcccgtctc
     4741 gggcaggccg cgcacgacga cattgctgcg cttggtcggc tcgtccagct tcggcagcga
     4801 attcagcacc acgacttcct tcgagacgag cgcacgccca tccggaccgc gcgctacctc
     4861 cggcgaggtc tcgatgagcg acgcctggtc acgcaggaga ccgctctgca cttcagactg
     4921 actgccctgg tggatcacca cgacattgtc cgccgagccg gtcgacacca gcgtcgactt
     4981 caggccggca tcgagcatca gcacggccgc aaacacgaac accaccagcg ccatgccgcc
     5041 ggcagtcagc gccgtcgtga gtttccgcac ccacaggttg cgcacgacat aggagaaggg
     5101 aatcttcatg tgtggtgtac gcgaccgctg cgcggtcgca cgtgcaccac acatgctagg
     5161 aggtgtgggc ctcccttcgg agcggccggg cgtgcggccc attcacaacg gcacgggtgc
     5221 atcaacctgc gattgccgca gccttgcgaa ctcgcgctag ctcgcgcgca gcgcgcgctt
     5281 tgctctcccg aggcgcggct gactacgtgc ggggccgaag tggccttccc cgcccgtagc
     5341 cgttgaagtc ggtccgtacg cccggccgcc cgaagggagg cccgctgatc ggggtcgcgc
     5401 gaggtgcacg cgcgactgcg aagctgtcgc gtacaccgag cgcgcctagg cgacatgccg
     5461 gaggccatcg acgatcttca cgcgcgcggc gcggatcgcg gggacgatgc cggcgacgac
     5521 gccaaccacg agcgcgcagg ccgcctgcag gtagaacgta tccgccacca gcggcatctt
     5581 tgcgaagacg ctcactgtcg cggcatggaa gatcctgacg acgggcgtcg tcagcagcat
     5641 gccgatggcc gtgccgatca gcgctagcag cattgcctcg cccatgatca tcgccgccac
     5701 cgtcccaggc ttgaatccca acgccttcag tgtcgcgtac tcgggcagcc gctcgcgtgc
     5761 tgtcatcgcc atcgtattgg cagccaccgc catgatgatc aggatgacga cgtagctcac
     5821 cccctggatc gccacgacga tggtctcgat catcgcgacg aagccgagcg tgaaagcctt
     5881 ttcggtctcc gtcagggttt cggccagaga attcttgaac tccgcgtcga tgtcgcgcga
     5941 gatattcgcc gcggcctgcc cgtcggcgat gccgacgatg aacacgccgg cctggtcggc
     6001 ccggcgcgga aacagtttct tgatctgctc gttgaggtag tcgaagtgaa agtacatctg
     6061 gcgcgtgatc gtcgtctcgt ccttcgcctc gtagatcgcg cgtacggtga actcccagtt
     6121 gccgggatat atcccgctct tcagcggaat cgtgtcgccc accttgaatc cgtagagatc
     6181 cgccagctgg cggcctatcg ctgcgccccg gcggtcgcgc aggaaggcct ggtactcctc
     6241 gggctggaag atgaactccg ggtacatcgg gaagtaggtc ggcgcatcga cggcaaactg
     6301 ggcaaagaaa ttcttcggct ccttgtagat gccgccgaac cagttcgaca cgctgactcc
     6361 ggtcaccccc gggaccgcgc ggattttttc ccggtagtac atcggcatcg cgaaagtgag
     6421 cgagatcgcg ttgcgcgaaa tcagccgcgt ggccgaggcg gcgtccgcgc ccgcatacca
     6481 ggcacgcacc acggtctgca gcagcccgaa agcgaggatc gcaatgacca ggccgaggat
     6541 cgtcagcgct gaacgcagcc gcgcgcgaaa gacattgcgc agaacgaact tcaggaagaa
     6601 cacggcgcgt ccttgttccg cgctagtgcc cgaacggggc cgtgtcggtc agctggccct
     6661 tgtcgaggta gatcaggtgg tgcgcggcat cggccgagcg cggatcgtgc gtcaccatga
     6721 cgatggtctt gcccagttcg ctgttcagcc ggtcgagcag gccgaggatg tccgccgccg
     6781 actgtcggtc gaggtcaccg gtcggctcgt cggcgattac cagcaacggg tccgtcacca
     6841 gcgctcgcgc gatcgccacg cgttgctgct gaccaccgga cagctcgttc ggatagtgct
     6901 tcatccggtc ggtcagcccg accatgccga gcaacagctc gacccgctcg cgccgttccg
     6961 agcgcgacag cgacgtgagc tgcagtggca gttcgacatt ttcgaacgcg gtgagtaccg
     7021 gcatcaggtt gtagaactgg aaaatgaaac cgaccgtacg cgcacgccag tcggccagtt
     7081 cggtgtcgcc aagctgcgtg atgtcctcgc ccgccacggc gagccgcccg gcatcgggct
     7141 tgtcgattcc ggcgaccagg ttgagcagcg tgctcttgcc cgaccccgac ggacccatca
     7201 tcgcgacaaa gtccccttcg cggatcacga ggttgatgtc gttcagcacc ggcacgacct
     7261 gctccccgcg aacgtaggac ttcgacacgt gctcgatctc gaccagcgcg cgccggtcga
     7321 tccccgcctc ggcgtgcggc atggttaggg tcggctcatg tcaatggcgg gagggcaggc
     7381 cctatttctt cactgcctcg acgagggcgc ccgcagccaa gcgttccggc gggttcgcca
     7441 ccacccgcgt acccgctgcg acgccctcga tgcgcacctg gtcgccgatc tttccgcccg
     7501 ctttcaccgg ggccggcttg accgtgttgc ccgcgtcgac aacgaacacg acctccgcgc
     7561 catcacgctt gacgatggca tcggcgcgca gggccggcac cggcttgcgc tcttcgggtg
     7621 tcagcgcgcg cgagaggaac gaaatcttcg cgctcatgtc cggcagcacg cgcgcatcgc
     7681 gctcgacgaa cttcaccttg accagcagtg tcgccttgct gcgatccacg gtcggcacca
     7741 cgcgactgac ctcgcccagc agccgcagtt cgggaaaggc gtcgagctgg atctcggccg
     7801 gctgtcccac cgatatcttg gcgatcgagg attccgagac atcggcttcg acttcgagtg
     7861 tttccatgtc ggccatcgtg acgacggcgc cggtcgtgcc cgacgccgag gaaaacggcg
     7921 tgacgatgtc gcccacgttg gcgctcttgg tcagcacgat gccggtgaag ggcgcacgga
     7981 tcagcgtctg gtcgtacgcg acgctcgtca cgcgatgatt ggcttgagcc acggcaattt
     8041 gcgccttcag cgtatcgatc tgtgcgcgcg tcttgttgta gcgcgcctcg gccgtatcga
     8101 gcgccgatcc cgaaatgaag ttctgccgcg ccaggtcctg cgaacgcttg agcgcggacc
     8161 gcgcgtcgtc gagctcggcc tcgccctgca cgaggttggc tcgtgccgcc tggacctggg
     8221 ccgcggcctg gtcgcgctgc gcggcgacat cgcggttctc gagccgcgca atcacgtcgc
     8281 cttccttgac catctgccct tcctggacgc cgagccattc cagccgaccg gttcccttgg
     8341 acgagaccga tgcgcgtcgt tgagccacca cgcggccggt cgcgttcagc accgatatcg
     8401 aggtcgaggg gtaggccgcg gcgacagttg cgaattccac cggcagcgcg gcactgcggc
     8461 tgcgcgcgaa ccaggcgacg gcgagcagca cgacgaccac cagcagccag acccaccagc
     8521 gcttccacag cggcctgcgc cgacgctgtg ccggcgcagc cgatcggtcg atcgcaagcc
     8581 gggcgagtga ggtcggtgca gcgggagtgt cggccatgaa caagctcgga aacggcggac
     8641 cgacatgcta atggaaggcg cttgtggggc ggcgcgcgtc ggtacgcgat agttcgcaac
     8701 gccgtgtcgc cggatcgggc cgcgcggtcg cccgcgggcc accgccctac ttgccgatgc
     8761 agaaccggct gaaaattacg ccgagcaggt cgtcggcgct gaactcgcca gtgatgctgg
     8821 cgagctggtt ctgcgcgaga cgcagttcct cggcaaacag gtccagcacc gcgtcgcgcc
     8881 gcgccgcatg tccgtccgcg gcttccagat gaatgcgcgc ctgcgccagc gcctgaagat
     8941 gacgctcgcg ggcgagatac acattctcgc cgtcggcagt gcgatcccag cctgccagcc
     9001 gatacagtcg ctcgcgcaac agatcgatgc cctggccgtc gcgagccgag aggtggaccg
     9061 ccgcgtcctc gctgtgtggc agagcgcccg tcaggtcgat tttgttgacc acggtgagca
     9121 gcgggacgcc acgcccggca cgcgcggtga cgcgcgcaag gacgtcctcg tcggcgctcg
     9181 gtcgcagcgc atccaccagg tgcaggacga catcggcacg ctccacttca gccagcgtgc
     9241 gcgcgatgcc cagcgtctcg acttcgtcgg tcgactcgcg cac
//
LOCUS       DATIRT010000076         2445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1483460, whole genome shotgun sequence.
ACCESSION   DATIRT010000076 DATIRT010000000
VERSION     DATIRT010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1483460"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..1791
                     /locus_tag="VLN25_01920"
     CDS             79..1791
                     /locus_tag="VLN25_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 catalytic subunit"
                     /protein_id="HSQ09362.1"
                     /translation="MQLTGAEIVVRALQDEGVTHVFGYPGGAVLYIYDEIFKQDKFQH
                     ILVRHEQAAVHAADAYSRSSQRVGVALVTSGPGVTNAVTGIATAYMDSIPVVIITGQV
                     PTHAIGQDAFQECDTVGITRPCVKHNFLVKDVRELATTIKKAFYIAQTGRPGPVLVDV
                     PKDVTIARTEYHYPREVDMRSYKPVLKGHQGQIKKAVQLLLAAERPMIYTGGGVILSD
                     SADLLNRLVNLLGFPCTSTLMGLGGFKASDRKFMGMLGMHGTYEANMAMQNCDVLLAV
                     GARFDDRVIGNPKHFASSPRKIVHIDVDPSSISKRVKVDIPIVGDVREVLIELLSQLE
                     AATTKPDAEALKLWWTQIEEWRKRDCLRYEQNGGPIKPQYVVEKLWELTKGDAFITSD
                     VGQHQMWAAQYYRFDKPRRWINSGGLGTMGVGLPYAMGVQMANPGAQVACITGEASIQ
                     MCIQELSTCKQYRLTPKIVNLNNRYLGMVRQWQQIDYGSRYSESYMDALPDFVKLAES
                     YGHVGIRIEDPKEVEGALKDAFGKYKDRLVFLDFITDQKENVWPMVRAGKGLTEMLLG
                     SEDL"
     gene            1791..2282
                     /gene="ilvN"
                     /locus_tag="VLN25_01925"
     CDS             1791..2282
                     /gene="ilvN"
                     /locus_tag="VLN25_01925"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HSQ09363.1"
                     /translation="MRHIISVLLENEPGALSRVVGLFSARGYNIETLTVAPTEDASLS
                     RMTIVSVGSDDVIEQITKHLNRLIEVVKVVDLTEGDYIERELMLIKLRAIGKEREEVK
                     RMADIFRGRIIDVTERTYTIELTGNSGKLDAFIQAVDRASILETVRTGGSGVGRGERV
                     LRV"
     gene            2311..>2445
                     /locus_tag="VLN25_01930"
     CDS             2311..>2445
                     /locus_tag="VLN25_01930"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="catalyzes the formation of
                     (R)-2,3-dihydroxy-3-methylbutanoate from
                     (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and
                     isoleucine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HSQ09364.1"
                     /translation="MKVFYDKDADLSLVKGKKVTIVGYGSQGHAHAQNLADSGVKVTI
                     A"
BASE COUNT          513 a          743 c          774 g          415 t
ORIGIN      
        1 cccgccgcac cgcgcgccac aagcgtgcat ggacggcatc cgatcaattt cccgtggact
       61 ttgagaggag ttagcgaaat gcaactcacc ggcgcagaaa tcgttgtacg agcgctgcag
      121 gacgaaggcg tcacacacgt ctttgggtac cccggagggg ccgtcctgta catctacgac
      181 gagatcttca agcaggacaa gtttcagcac atcctcgttc ggcacgagca ggcggcagtg
      241 catgccgccg atgcctattc acgttcgagc cagcgggtcg gcgtggcgct ggtcaccagt
      301 ggccccggcg tcaccaatgc cgttaccggc atcgcgacgg cctacatgga ttcgatcccg
      361 gtcgtcatca ttaccggcca ggtgccgacc catgcaatcg gccaggatgc cttccaggag
      421 tgcgacaccg tcggcatcac gcggccgtgc gtgaagcaca actttctcgt gaaggacgtg
      481 cgggaactcg cgaccaccat caagaaagcg ttttacatcg cacagacggg acgaccgggg
      541 ccggtgctgg tcgatgtccc gaaggacgtg acgatcgcgc gcaccgagta ccactacccg
      601 cgcgaagtcg acatgcgctc gtacaagccg gtgctcaagg gccaccaggg gcagatcaag
      661 aaggcggttc aactgctgct cgcggccgag cggccgatga tctacaccgg cggcggcgtg
      721 attctctccg actcggccga tctgttgaac cgactcgtca atctgctcgg gtttccctgc
      781 accagcactc tgatgggcct gggcggtttc aaggccagcg accgaaagtt catgggcatg
      841 ctgggcatgc acggcaccta tgaagccaac atggcgatgc agaactgcga tgtgctgctg
      901 gccgtcggtg cccgcttcga cgaccgcgtg atcggcaacc cgaagcactt tgcgagcagc
      961 ccacgcaaga tcgtccatat cgacgtcgat ccatcgtcga tctccaagcg ggtcaaggtc
     1021 gacatcccga tcgtgggcga cgtgcgcgag gtgctgatcg aactgctctc gcagctcgag
     1081 gcggcaacca cgaagccgga tgcggaagcg ctcaagctgt ggtggaccca gatcgaggag
     1141 tggcgaaagc gcgactgcct gcgctatgag cagaacggcg gaccgatcaa gccgcagtac
     1201 gtcgtcgaga aactgtggga gctcaccaag ggcgatgcct tcatcacctc ggatgtcggc
     1261 cagcaccaga tgtgggcggc gcagtactac cgcttcgaca agccgcgccg atggatcaac
     1321 tccggcggcc ttggcacgat gggcgtcggc ctgccgtacg cgatgggagt gcagatggcc
     1381 aatccgggcg cgcaggtcgc gtgcatcacg ggcgaggcct ccattcagat gtgcatccag
     1441 gaactgtcga cctgcaagca gtaccgcctg acgccgaaga tcgtgaatct caacaaccgg
     1501 tacctcggca tggtgcggca atggcagcag atcgactacg gcagccgcta ctcggagtcg
     1561 tacatggatg cgctgccgga cttcgtgaag ctggcggaaa gctacggcca cgtcggcatc
     1621 cgcatcgagg acccgaagga ggtcgagggt gcgctcaagg acgcgttcgg caagtacaag
     1681 gaccggctgg tgttcctcga cttcatcacc gaccagaagg aaaacgtctg gccgatggtg
     1741 agagccggta aaggactgac cgagatgctt ctcggctcgg aggacctgta atgcgccaca
     1801 tcatttctgt cctgctggaa aacgagcccg gtgcgttgtc ccgcgtggtg gggctgttct
     1861 cggcgcgcgg ctacaacatc gagacactga cggtcgcgcc caccgaggat gcgtccttgt
     1921 cccgcatgac catcgtcagc gtcggttcgg acgacgtgat cgagcagatc accaagcacc
     1981 tgaatcggct gatcgaggtg gttaaggtcg tcgacctcac tgaaggcgac tacatcgagc
     2041 gcgagctgat gctgatcaag ctgcgggcga tcggcaagga gcgcgaggaa gtcaagcgca
     2101 tggctgacat cttccgtggc cgcatcatcg acgtgaccga gcgcacctac acgatcgagc
     2161 tgacaggaaa ctccggcaag ctcgatgcct ttatccaggc cgtggaccgg gcgtccatcc
     2221 tggaaaccgt gcgcaccggc gggtccggtg tcggtcgcgg cgaacgagtt ctgcgagtct
     2281 gaacaaacaa tcgagaaggg gagtcagcca atgaaggttt tctacgacaa ggacgccgac
     2341 ctatcactgg tcaagggcaa gaaggtcacg atcgtcggtt acggttcgca gggccacgcg
     2401 cacgcgcaga acctcgccga ctccggcgtc aaggtaacga tcgcc
//
LOCUS       DATIRT010000077         8495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_500149, whole genome shotgun sequence.
ACCESSION   DATIRT010000077 DATIRT010000000
VERSION     DATIRT010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8495
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500149"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..441)
                     /locus_tag="VLN25_01935"
     CDS             complement(22..441)
                     /locus_tag="VLN25_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HSQ09365.1"
                     /translation="MSASKPSAPSPSSIVLHTASRVLEVAFDDGATFRMPFEFLRVHS
                     PSAEVRGHGAGQETLQEGKRDVTINELEPVGHYAIKPTFSDGHDSGIFAWDYLYELGM
                     NQEQLWSEYLERLQAAGASRDPSATPVTAPGRSCGHH"
     gene            616..1689
                     /locus_tag="VLN25_01940"
     CDS             616..1689
                     /locus_tag="VLN25_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HSQ09366.1"
                     /translation="MSSSPILQVRPLGFPWETADPFLFCVYHDDAYPRGNGRLGPADS
                     LAGRAIGQDFSRKDGWSMYHGDSVPGFPAHPHRGFETVTVVRKGLIDHADSLGAAARY
                     GRGDVQWLTTGRGVVHSEMFPLLDDAEPNPLELFQIWLNLPAASKMSAPHFTMLWAED
                     IPRRVVADSRGATTEISVIAGALRDGSASAKESVVQPLAPPPDSWAAREQADIAIWTI
                     RMSPGAHWTVPAANAGTRRNLYLFKGGPVTVGGERVSDPAAIEVRADLSTELINGSSV
                     SEFLLLQGRPIGEPVVQHGPFVMNTQAEIAQTFADYQRTRFGSWPWPDAAPVHGREAA
                     RFARHPDGHGESPAERAARAGVK"
     gene            complement(1845..1920)
                     /locus_tag="VLN25_01945"
     tRNA            complement(1845..1920)
                     /locus_tag="VLN25_01945"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1885..1887),aa:Arg,seq:ccg)
     gene            2199..3275
                     /locus_tag="VLN25_01950"
     CDS             2199..3275
                     /locus_tag="VLN25_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSQ09367.1"
                     /translation="MSSDHHTSPIKTPRQLITVVILAFVVPVIVIILLATFVTGQKVG
                     GAGTDAMTPEAIAERLRPVGTIAFAGAASGPRTLQDGEAVYKLACGACHASGAAGAPK
                     TGDAGAWAPRIKQGYDTLVKHAVEGFKAMPAKGGNADLDPIEVARAVVYMSNLSGAKF
                     KEPAAPTQAVAKSSVRTGEQIVQMSCCNCHNAGLNGAPKIGDRAAWTKRVSHGVDAVA
                     ALAIKGHGGMPARGGMADLTDAELKSAVQYMFNRSTGAPTTTTAPVAAAAAAPEAAAT
                     PAKADGAKIYAMGCNACHAAGIAGAPKFGDKAAWATRGKLGVDALVASVVKGKGAMPP
                     KGAVASASDAELRAAVEYMLAAAK"
     gene            complement(3483..5513)
                     /locus_tag="VLN25_01955"
     CDS             complement(3483..5513)
                     /locus_tag="VLN25_01955"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HSQ09368.1"
                     /translation="MTRVRFLQVLLDLPLESPLDYANTEVAEAGAADALVGLRCVVPL
                     GRRKAVGLVVGTSSVTALEPARIKPVSRLLDEIGPVDAHWLGLTRFASDYYQQAWGEV
                     ALPALPPTLRQLPGPRYAQSLARLRSAGSEVATTAESIQRPALTHEQSLAVERLTAAA
                     GFAPSLLFGVTGSGKTEVYLSVIEQTLAARPESQALLLVPEINLTPQLESQVRARFPA
                     YGVVALHSGLAAGERAAAWLGAHEGRARVVIGTRLAVFASLPRLGLIVVDEEHDPSYK
                     AGEGVRYSARDLAVKRAQLLGIPLILGSATPSLESWSQARAGRYQLHTLTQRATTEDW
                     VAPRIELIDPGAAGLDSGVAPAIESALSESLQRGEQSLVFLNRRGYAPVVACEACGWL
                     SNCPNCAAYAVFHKPDRTLRCHHCGWNSPVPRACPTCGNSALQGIGRGTQRIEESLQE
                     LLPTARVLRIDRDSTRRRDSAARAFDSVHRGDIDVLVGTQMVAKGHDFRNVALVVVLN
                     ADSQLASQDFRASERLFATLVQVVGRAGRSGGPSRALLQTRFPQHPLFAALVRQDYAA
                     FADALLAERSRAGMPPTSHQALLTAEARRMNDAVAFLRTAVDLAAAPLDVRIYDPVPM
                     ALARRAGIERAQLLVESAQRGPLHAFLRAWLGDLRARKSSVRWQIEVDPAEI"
     gene            complement(5846..6928)
                     /gene="hemE"
                     /locus_tag="VLN25_01960"
     CDS             complement(5846..6928)
                     /gene="hemE"
                     /locus_tag="VLN25_01960"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HSQ09369.1"
                     /translation="MRPSNDTLLRALLREPTDYTPVWLMRQAGRYMAEYNATRKRAGS
                     FLNLAKSPAMATEVTLQPVERFPLDAAILFSDILTVPDAMGLGLYFAEGEGPKFERPV
                     RTEADVAALSAPDPELSLGYVMDAVREIRKALANRIPLIGFSGSPFTLACYMIEGGGS
                     DDFRQIKTMLYARPDLLHRILKVNARAVTDYLNAQIAAGAQAVMIFDTWGGALADGAY
                     QEFSLAYMREVVSQLQREHEGERVPSIVFTKGGGLWLEQIAAIGCDSVGLDWTVNLGQ
                     ARARIDDRCALQGNLDPMALFAPEEPLRAQVRRVLDAFGSPRRGDGRLGGHVFNLGHG
                     ISQFTPPESVAVLVDEAHRHSRTLRR"
     gene            7018..7272
                     /locus_tag="VLN25_01965"
     CDS             7018..7272
                     /locus_tag="VLN25_01965"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HSQ09370.1"
                     /translation="MHVQPGARRSEIVGMHGGRLKLAVRALPTEGRANSAAIKLIAER
                     LACRVSAVRIAAGESSRDKRLQVDTALHTSDQIIAKLQPK"
     gene            complement(7318..7536)
                     /locus_tag="VLN25_01970"
     CDS             complement(7318..7536)
                     /locus_tag="VLN25_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09371.1"
                     /translation="MNKLLAAMFAAAFATGVAYAADPVKKPTADECKKAAEAKKELAG
                     CEKPKAAPAAAPAAAPAAAPAAAPAAKK"
     gene            complement(7725..8084)
                     /locus_tag="VLN25_01975"
     CDS             complement(7725..8084)
                     /locus_tag="VLN25_01975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HSQ09372.1"
                     /translation="MRLAPRDPALEAWKRAAALRIHQANQKQLFEGRPHHLLQAVIVV
                     EATVDRGGNVVGSRVTRSPKIQSLDRMALASLKAASPLPAPPSTLLVRGNLVFSETWL
                     VQNDGRFQVRTLALPQE"
     gene            complement(8291..>8495)
                     /locus_tag="VLN25_01980"
     CDS             complement(8291..>8495)
                     /locus_tag="VLN25_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSQ09373.1"
                     /translation="RLAGIDCRTSMQESTDVFRGVLAAADQAGAGLIVMASHGRQGLS
                     ARVLGTETQRLLDHTLIPVLIVR"
BASE COUNT         1563 a         2757 c         2796 g         1379 t
ORIGIN      
        1 gctcatcgcg gctcttcgac gctagtgatg accacaactc cggccagggg ccgtcaccgg
       61 tgtggccgac ggatcgcggc tcgccccggc cgcctgcaga cgctcgaggt actcggacca
      121 aagctgctcc tggttcatgc cgagttcgta caggtaatcc caggcaaaga tgccgctgtc
      181 gtgtccgtcg gaaaacgtcg gcttgatcgc atagtgtccg accggctcca gctcattgat
      241 cgtcacgtcg cgcttgcctt cctgcagcgt ctcctggccc gcaccatggc cgcgcacttc
      301 ggccgacggc gaatggaccc gcaggaattc gaacggcatc cggaacgtcg cgccgtcgtc
      361 gaacgcgact tcgagcacgc gcgaggcggt atggagcacg atgctggacg gggagggcgc
      421 gctgggtttg ctggcggaca ttgggaagca gaattgggaa ggggactcgg aaaggaatcg
      481 agaaggagaa tcgtcgaagt cgcgatcgct cggcgacgga caatgatcgt acaccgcggt
      541 cccggtgcga cgagcgaacc cctaaagcgt cacgttgtca gagtaggcgg ccttacacgt
      601 tacgaatgaa ccctcatgtc gagtagtcca atcctgcaag tcagacctct gggcttcccg
      661 tgggaaaccg cggacccctt cctgttctgc gtttatcacg acgacgctta tccccgcggc
      721 aacgggcggc tcggccccgc ggactcgctc gcgggccgcg cgatcggcca ggacttcagt
      781 cgcaaggacg gctggagcat gtaccacggt gattccgtgc cgggcttccc cgcgcatccg
      841 caccggggat tcgaaaccgt gacggtcgta cgcaagggac tcatcgacca cgccgactcc
      901 ctgggtgccg ccgcgcgcta tgggcgcggc gacgtgcagt ggctcaccac gggccgcggt
      961 gtcgtgcatt cggaaatgtt tccgctgctc gatgacgctg agcccaaccc gctggaactc
     1021 ttccagatct ggctcaacct gccggccgcc tccaagatgt cggcgccgca cttcacgatg
     1081 ctctgggccg aagacattcc ccgccgcgtc gtagccgaca gccgcggcgc gacgaccgag
     1141 atctcggtga tcgcgggtgc gctgcgtgac ggctccgcgt ccgcaaagga aagcgtcgtg
     1201 cagcctctcg ccccaccgcc cgattcctgg gccgcacgcg agcaggccga catcgccatc
     1261 tggacgattc gcatgagccc cggcgcgcac tggactgttc cagccgccaa cgcagggacg
     1321 cggcgcaatc tgtatctgtt caagggcggg cccgtcactg tcggcgggga aagagtcagt
     1381 gaccctgcag ccatcgaagt ccgtgcggac ctgtcaacgg aacttatcaa cggcagttca
     1441 gtaagcgagt tcctgctgct gcagggacgg ccgatcggcg agccggtcgt gcagcacggc
     1501 ccgttcgtca tgaacacaca ggccgagatc gcgcagacgt tcgccgacta ccagcgcact
     1561 cgattcggca gttggccctg gcccgatgcc gcgcccgttc acgggcgcga agcagcacgc
     1621 tttgcgcggc atcccgatgg tcacggcgaa agtccggcgg agcgcgctgc tcgtgcaggg
     1681 gtgaagtaat cgcgacacga tcgcgtccga ccattgacgc aggatcgagc gagacgcgac
     1741 aagggagacg caacaaccct gttgctgagg ccggaacccc ggtgtctgca gaccaagcag
     1801 aagctcgaag ccgcgatcaa cacccaagcg aaggacaaca aagttggcgc gcctggcggg
     1861 attcgaaccc acgacccctg gcttcggagg ccagtactct atccactgag ctacaggcgc
     1921 gtgcctgcga tgcgaccgca caccctgcgc gagctgtcgt tccggcgcgc tgggcacggc
     1981 acccggaggg tgcgcgagcg tggggcggat ggtaacgggt ttcgctgcgt accgctcgcg
     2041 gatacgcgcg caacgcttgg cgtgcaggca gtcggagatg gctcgcaagc tatacttctg
     2101 cgcttcgcga ggcgatcgtg cctcgcagtt tcagcgctct cgatttcgca ctccgtaccg
     2161 ccccgagcag cgccgtcgca cccgcagcca ggagccctat gtcgagcgac caccacactt
     2221 cgccgatcaa gacgccgcga cagttgatca cggtcgtcat tctggccttc gtcgtcccgg
     2281 tcatcgtgat catcctgctg gccacgttcg tgaccgggca gaaggtcggc ggggccggta
     2341 ccgacgcgat gacgccggaa gcgatcgcag agcgcctgcg tccggtcggc accattgcct
     2401 ttgccggcgc tgccagtggc cctcgtactc tccaggatgg cgaggccgtg tacaagcttg
     2461 catgcggtgc gtgccacgcc agcggcgctg caggcgcacc gaaaaccggc gatgccggtg
     2521 cctgggcgcc gcgcataaag cagggctatg acacgctggt caagcacgcg gtcgagggtt
     2581 tcaaggcgat gccggccaag ggtggcaatg ccgatctcga tccgatcgaa gtcgcgcgcg
     2641 ctgtcgttta catgtccaac ctgtccggcg ccaagttcaa ggagcctgcg gcaccgaccc
     2701 aggccgtggc caagtcgagc gtgcgcaccg gggagcagat cgtgcagatg tcatgctgca
     2761 actgccacaa cgcgggcctc aacggcgcgc caaagatcgg cgatcgtgcg gcgtggacga
     2821 agcgggtgtc acatggtgtc gatgcggtcg cggcgctcgc catcaagggt catggcggca
     2881 tgccggctcg cggtggcatg gcggacctca ccgacgccga actcaagtcg gcggtgcagt
     2941 acatgttcaa tcggtcgacc ggcgcaccga ctacgaccac cgctcctgtt gcagcagcgg
     3001 cggctgcccc ggaggctgcg gcgactccgg ccaaggcgga tggcgcgaag atctacgcga
     3061 tgggatgcaa tgcctgccac gctgccggca ttgccggggc tccgaagttc ggcgacaagg
     3121 ccgcctgggc tacgcgcggc aagcttggcg tcgatgcgct cgtcgcctcc gttgtcaaag
     3181 gcaagggtgc gatgccgccc aagggcgcag tggcgagtgc gagtgacgca gaactgcgtg
     3241 ccgcggtgga gtacatgctg gcggctgcca agtagctcgg caagatgttc aaacgagacg
     3301 ggcgctgggg cgcccgtttt cattgcgatc gctggccgcg agccgcttca gggattggac
     3361 tgccggtaca caaggcacgc tatggtctgc agttcgtcct tcggcagatc acggcgagcg
     3421 cgtagccaag cggaccgtcg atcaaccgca cacacacggc tatgcgcagt tattccacgc
     3481 tctcagattt ccgccgggtc gacctcgatc tgccagcgta cgctcgactt cctcgcacgc
     3541 aggtcgccga gccaggcgcg caggaaggca tgcagcggcc ctcgctgagc cgactccacc
     3601 agcagttgcg cgcgttcgat cccggcgcgc cgcgcgagcg ccatgggcac cggatcgtag
     3661 atgcgcacgt cgagaggagc cgccgccagg tcgaccgcgg tcctgagaaa agcgacggca
     3721 tcgttcatcc gacgcgcctc ggccgtcagc agggcctgat gactcgtcgg cggcatgccg
     3781 gcacgactcc gctcggccag cagcgcgtcg gcaaaagccg catagtcctg ccgcacgagt
     3841 gcggcaaaca gcggatgctg cggaaagcgg gtctgcagca gggcccggct cggcccgccg
     3901 ctgcggcccg cccggccgac cacctgcacc agcgtcgcga acaggcgctc cgacgcacgg
     3961 aagtcctgcg aagccagttg actgtcggca ttcagcacga cgacgagcgc cacgttgcgg
     4021 aagtcgtgcc ccttggcaac catctgcgtg ccgaccagca catcgatgtc acctcgatgc
     4081 acggagtcga acgcgcgcgc cgccgagtcg cgccgccgtg tcgagtcccg atcgatgcgc
     4141 agcacacgcg ccgtcggcag gagctcttgc agcgattcct caatgcgctg tgtgccgcga
     4201 ccgatgccct gcaacgcgct gttgccgcag gtcgggcacg ctcgcggcac cggactgttc
     4261 cagccgcagt gatgacaacg caacgtgcga tccggtttgt ggaacaccgc gtacgcagcg
     4321 cagttcgggc agttcgaaag ccatccgcag gcttcgcagg cgaccactgg tgcatagccg
     4381 cgacggttta gaaacaccag tgactgctcg cctcgctgca gcgattccga cagcgccgac
     4441 tcgatcgccg gcgcgacgcc cgagtcgagc cctgcggcgc ccggatcgat gagttcgatg
     4501 cggggcgcaa cccagtcctc cgttgtggcc cgctgggtga gcgtgtgcaa ttggtagcgg
     4561 ccggcgcggg cctgcgacca gctttcgagc gatggcgtag ccgagccgag gatcagtgga
     4621 atcccgagca gctgcgcgcg tttgaccgcg agatcgcgcg ccgagtagcg cacgccctcg
     4681 ccggccttgt acgacggatc gtgttcttcg tcgacgacga tgagtcccag gcgaggcagc
     4741 gaagcgaaga ccgcgaggcg tgtgccgatg accacgcgcg cgcgtccctc gtgcgccccg
     4801 agccaggcgg ccgctcgctc cccggccgcc agtccgctgt gcaatgcgac gaccccgtag
     4861 gcgggaaagc gtgcgcgcac ctgcgactcg agctggggcg tgaggttgat ctccggcacc
     4921 agcagcagcg cttgcgactc cggacgagct gccagggtct gctcgatcac cgacaggtag
     4981 acctcggtct tgccactgcc ggtgacaccg aacagcaatg agggggcgaa tccggcagcg
     5041 gccgtcagcc tctctacggc aagcgactgc tcgtgagtca gtgcgggtcg ttgaatcgat
     5101 tccgctgtcg tggccacctc ggatcctgcg gaacgcagac gggcgagcga ctgcgcgtaa
     5161 cgcgggccgg gaagttgacg cagtgtgggc ggcagtgcag gcagcgccac ttcgccccac
     5221 gcttgctggt agtagtcgga agcaaagcgg gtcagaccga gccagtgagc gtcgaccggg
     5281 ccgatctcat cgagcagacg agaaaccggt ttgatacggg cgggctcgag tgcggtgacg
     5341 cttgacgtgc ccacgacgag accgactgct ttgcgacgcc cgaggggcac gacgcaacgc
     5401 aggccgacga gggcatcggc agctccggcc tcggcgacct cggtgttcgc ataatcgagc
     5461 ggcgactcga ggggcaggtc gagcaatacc tgcaggaagc ggacgcgtgt cacgctgtcg
     5521 accgacccaa tttctgacct aagacttcaa gttaaacagg taacccactg atttgaaatg
     5581 aaccgacaca tttgatctgc cagcctgtgg ataactttgt ggaaaaacgg tgggaagtcg
     5641 tcgcaaaccc ctgtaggaat tggcctcggc ccggatcggc gtccgtaaca cttctgtcaa
     5701 cattcatacg aatcaatgac ttagggctct gaaacgagcg cgcccgacgc ccatgccctg
     5761 cgaacccgca tggaatctcg ctgaacgcgc ttttgtgaat aagtcaagcg ctgacaacaa
     5821 accgcgccac attcgcagtg accactcacc tacgaagagt ccggctgtgg cgatgtgctt
     5881 cgtcgaccag tacagcgacc gactccggtg gcgtgaactg cgaaattccg tgcccgagat
     5941 tgaagacatg gcctccaagc cggccatcac ctcggcgcgg tgaaccgaag gcatccagca
     6001 cgcggcgcac ctgcgcacgc agcggttctt ccggcgcaaa tagggccatc ggatcgaggt
     6061 tgccctgcag ggcacagcga tcgtcgatgc gcgcgcgcgc ctggcccagg ttgacggtcc
     6121 agtccagccc gaccgaatca caaccgatcg cggcgatctg ttcgagccac agaccgccac
     6181 ctttcgtgaa gacgatgctc ggaacgcgct cgccctcgtg ctcgcgctgc agttgcgaga
     6241 caacctcgcg catataggcc agcgaaaact cctggtaggc gccgtcggcg agcgcaccgc
     6301 cccaggtgtc gaagatcatc acggcctggg ccccggcggc gatctgtgca ttgaggtaat
     6361 cggtgacagc gcgggcattg accttcagga tccgatgcag caggtccggg cgcgcataca
     6421 gcatggtctt gatctgacgg aagtcgtcgg atccgccacc ttcgatcatg tagcaggcga
     6481 gcgtgaacgg gctgcctgaa aagccaatca gcggaattcg attcgcgagc gctttgcgga
     6541 tctcgcgcac cgcatccatc acgtagccga gcgacagttc cggatcggga gcggaaagag
     6601 cggcgacatc cgcctcggtc cgcaccggcc gttcgaactt gggcccttcg ccttcggcga
     6661 agtacagtcc cagacccatc gcgtcgggca ccgtcaggat gtcagagaac aggatcgcgg
     6721 catccagagg aaaccgctcg acgggctgga gcgtgacctc ggtggccatt gccggcgact
     6781 tggcgaggtt caggaagctg cccgcacgct tgcgcgttgc gttgtactcg gccatgtagc
     6841 ggccggcttg acgcatcagc cacaccggcg tgtagtcagt cggttcgcgc agcaacgcgc
     6901 gcagcaacgt atcgtttgaa ggtctcacgg agaagccggt tgaaagcaaa ccgcgattct
     6961 gtcacgccgc cggttccttc ctggctgcgt ccgaacagcg agggtttcac tcttgaggtg
     7021 cacgtacagc cgggggctcg ccgttcggag attgtcggca tgcatggggg tcgtctgaag
     7081 ctggcggtga gggcactgcc gaccgagggt cgggccaact cggcagctat aaagctgatt
     7141 gcggagcgac ttgcctgccg cgtctccgcc gtgcgtattg cggcgggtga gagcagccgc
     7201 gacaagcggc ttcaggttga taccgccttg cacacttcag accagataat cgcgaagctg
     7261 cagccaaagt aaaaagggca ggccatgcgg cctgcccttc aagcgaaccc gagctgatta
     7321 cttcttggca gcgggagcag cggcaggcgc agcggccgga gcagcagccg gagcagcagc
     7381 gggcgccgcc ttcggctttt cgcagcccgc caattccttc ttggcttcgg cagctttctt
     7441 gcactcgtca gccgtcggct tcttgacggg gtcggcagcg taggcaacgc cggtcgcgaa
     7501 cgcggcggcg aacatggcag caagcagttt gttcatgtca atagtccctg gaacggttat
     7561 cgaagattag agcgggaggg tcggccccga aagggatccc aagctccgct atggccaacg
     7621 ttcggccggc gtgaaggttg acacgaaagt gacgtcgcct agtggaaaac ccgctcgaac
     7681 gggcgatttc tatcgccaga accgcgggga atgatgcgtt tccgctactc ctggggcaga
     7741 gccagggtcc ggacctggaa acgcccgtcg ttctggacca gccaggtttc ggagaaaacc
     7801 agattgccgc gcaccagcag tgtcgacgga ggcgctggaa gaggggacgc ggccttcaac
     7861 gacgctagcg ccatccggtc cagcgactgg atcttgggcg accgcgtcac gcgggacccg
     7921 acgacattgc caccacggtc aaccgtcgcc tcgaccacga tgacggcctg cagcaggtga
     7981 tggggccggc cctcgaacaa ctgcttttga ttggcctggt gaatgcgcaa ggccgcggcg
     8041 cgcttccatg cctccagtgc agggtcacgc ggcgctagtc gcaccgcagt ccccgtgccc
     8101 gctgcagccg gcgccggggc gggggtggtc ggcgtggcgc tcgtaccggg gctgctcact
     8161 acaggcgggg tcgccggcgg ggtcggggcc ggagcaccgc acgccgacag caaacccacc
     8221 aacgtaaccg aggataggta cgacctccag gcggtggagc gtgcagggac gatggccatg
     8281 actgcctcct tcaacgaaca atgagtaccg gaatcagggt gtggtcgagc agccgctgtg
     8341 tttccgttcc gaggacccgg gccgacagcc cctgccgccc gtgcgatgcc atgacgatga
     8401 gcccggcgcc ggcttggtcc gccgcggcca gcactccgcg aaatacgtcg gtcgactcct
     8461 gcatcgaggt cctgcagtca atgcctgcca gacgc
//
LOCUS       DATIRT010000078        13405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_402264, whole genome shotgun sequence.
ACCESSION   DATIRT010000078 DATIRT010000000
VERSION     DATIRT010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13405
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402264"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /gene="phaR"
                     /locus_tag="VLN25_01985"
     CDS             complement(<1..412)
                     /gene="phaR"
                     /locus_tag="VLN25_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011402311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HSQ09374.1"
                     /translation="MATPVRLIKKYPNRRLYDTQTSTYITLADVKQLVLDNENFQVVD
                     AKSAEDLTRPILLQIILEEEAGGMPMFSATMLSQIIRFYGNAMQGMMGSYLEKNIQAF
                     IDIQGKLSEQSKAFYETNKIGPEMWTQFVNMQSPM"
     gene            561..731
                     /locus_tag="VLN25_01990"
     CDS             561..731
                     /locus_tag="VLN25_01990"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HSQ09375.1"
                     /translation="MCQNQSEFLGEFTRYRVRVGTTLLTADAPHLSATPIYSPGMGVQ
                     IGIDPDEVRVLA"
     gene            complement(789..1286)
                     /locus_tag="VLN25_01995"
     CDS             complement(789..1286)
                     /locus_tag="VLN25_01995"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HSQ09376.1"
                     /translation="MLEIRTQIDIAASASLVWGILTDFGSYRRWNPYMRSVTGQSQVG
                     NSIEITQDVVAFGASTFRATVTHVREPRELRWFGRWAVPGLYSAEHRYSIESLPGGGV
                     RLHNCEQYRGLALPFARHRLQQIIEPALAAMNLAVKRRAERAEEPKDSAALDSRQYAD
                     QDRRS"
     gene            1522..2496
                     /locus_tag="VLN25_02000"
     CDS             1522..2496
                     /locus_tag="VLN25_02000"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HSQ09377.1"
                     /translation="MRRRIILGSAIGVAFIAPAPTLARTPAVLHRPVQILLPFPSDVA
                     YSLARLIADGISQAVGQPVFIETRSGATGRVAGEVLKNAAPDGTTLAFFPIAVPVLAP
                     LVFRQLRFDPPRDFLAVTEIARFHYALAVAADHPARSVPEFIAWSRQHPARASVGTSG
                     AGTISHFFTVMINRAAKAELLHVPYRNVGVMATDVIGGQIAGGIDALATLIEFHRAGR
                     LRIIATTHVTRSALLPAVPTFAEQGLPGVQGVGWIGVFVPAGTPRPIIDILSRAILQV
                     LRTPQVRDQFAALAVEVTGTSPEEFAAIIATDTAHWAPIVRASGFSGD"
     gene            complement(2468..3727)
                     /locus_tag="VLN25_02005"
     CDS             complement(2468..3727)
                     /locus_tag="VLN25_02005"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HSQ09378.1"
                     /translation="MQLRFRAVSWFFSALMLGAGSPTQAQLSPEEIERVLSFGPWPPA
                     ATKDAGNRVSGNPAAVELGRRLFFDSRLSRVGYIACVTCHQPDRAWGDAKARAHGLAD
                     VDRNTPMLANLRLARWYGWSGASDSLWMASLRPILDERELDSTPAHIRHLYVREPELA
                     CWYGRAFGHPADDTRDDDVTVLVNSGKALAAFQETLITGRTPFDDYRDALARGDGATA
                     AAYPAAAVRGLQLFIGRGNCVLCHNGPNFSNGEFHVNGVGHFIGRGRADGGRFEGIEA
                     VKTHPYNLLSRFNDDTTRTNTTPTRNVVLSSRNWGEFRTPSLRNVAVTAPYMHNGSLD
                     TLQDVIRHYSELDQERLHADGENLLRALKLNDTEVEDLLVFLGTLTDRHGADRSRPSA
                     PAEECAVDKDARGAAVGIPNRLKIRKP"
     gene            complement(3746..4162)
                     /locus_tag="VLN25_02010"
     CDS             complement(3746..4162)
                     /locus_tag="VLN25_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HSQ09379.1"
                     /translation="MTAWGRRGAVVTAALSSAFGAAIGCEPAPIGADAARITGRIFVV
                     DWRVEPAPLRLSEFFTVVVSTCERAGQPVSSVKVDATMPAHRHGMNYAPTVTSEAGGR
                     FRASGLLLHMPGRWEITFDLAGDSTRETVRSIVELK"
     gene            complement(4159..4998)
                     /locus_tag="VLN25_02015"
     CDS             complement(4159..4998)
                     /locus_tag="VLN25_02015"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSQ09380.1"
                     /translation="MTRPLLLSVMAATAVASLAMPVAGESAESLPIFDAHVHYSHDAW
                     EMLPPEVALDLVRKAGVKRALVSSSDDDGQQRLVRLAPDVFLPSLRPYRRRGEISTWV
                     RDPSVIHYLEERLAKYRYVAVGEFHIYGADVDLAVPRRMIELARKHDLVLHSHSDADA
                     IERQFMQWPQARILWAHAGFVSPADIRAMLRKHRNLWCDLAFRSEHGAGGKLDPDWRA
                     LMLEFPDRFMVGTDTFTPERWPFIVEHARWSRAWLSDLPPDVAERIAYRNGEALFAGA
                     LGK"
     gene            complement(5035..5664)
                     /locus_tag="VLN25_02020"
     CDS             complement(5035..5664)
                     /locus_tag="VLN25_02020"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HSQ09381.1"
                     /translation="MVLIGMLDSPYVRRVAISLQLLGLQFEHRSVSVFRSFEDFRKIN
                     PVVKAPSLVCDDGEVLMDSSLILEYAEALSAPRRSLMPTDVAARQHALRLIGLALVAC
                     EKSVQIIYERSLRPEEKWHEPWIARVTGQLRAAYEALEVELRARPLPVSTSTIDQAGI
                     STAVAWHFTQQTLPELVLAANHPNLQLVSQRAEELPAFMAAPHGSGTFR"
     gene            5920..7185
                     /locus_tag="VLN25_02025"
     CDS             5920..7185
                     /locus_tag="VLN25_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008347187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HSQ09382.1"
                     /translation="MNPVWKATAIAVALAVGSVANVSAQQNIEKLKQMRASGTDPNIP
                     SIPQSGKNADALRENLKKVKLPPGFKIELFAIVPDARHMAIAPSTNMLFVGTRKTAVW
                     AVTDRNSDYVADEVKQFAPSLKFSVPNGVCWTKDGFLIVVEHNRVLNFPAAEFFYEGP
                     DVAVIEVVPQGKLIPEEEESYNHHARTCRVGPDGMLYITLGQPFNVQPRDKIAIFNKV
                     GIGGIVRMNPFDGSKREVYATGIRNSVGIDISPKDGSVWFTDNQTDGMGDDIPAGELN
                     RATKPGQFFGYPWKQGKTRITEHGYDKDPLPANITDPQVYFDAHAADLGLTHYTGKQF
                     PAKYQGGIFSAQHGSWNRTTPIGARVMFTSLKPDGTADKTELFADGWLDNTTGTYRGR
                     PVDVAVMKDGSLLVSDDFAGAIYRISYQP"
     gene            7190..7444
                     /locus_tag="VLN25_02030"
     CDS             7190..7444
                     /locus_tag="VLN25_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSQ09383.1"
                     /translation="MPRRYRTAVAVLATAVSSLLSSAHDAAAQDVTAGRRKAIACQNC
                     HGMDGLAKLPDAPHLAGQPAAYMVRSLRAYRSGERRNEVM"
     gene            complement(7662..8396)
                     /gene="phbB"
                     /locus_tag="VLN25_02035"
     CDS             complement(7662..8396)
                     /gene="phbB"
                     /locus_tag="VLN25_02035"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HSQ09384.1"
                     /translation="MAKVAYVTGGMGGIGTAICQRLHKEGFKVIAGCGPSRDFGKWLG
                     EQKALGLTFYASVGNVADWDSTVSAFSKAVAEHGPIEVLVNNAGITRDRMFLKMTPED
                     WKAVIDTNLNSMFNVTKQVVPGMVEKGWGRIIQISSVNGEKGQAGQTNYSAAKAGMHG
                     FTMALAQEVASKGVTVNTVSPGYIGTEMVNAIKPEVLEKIVATIPVKRLGTPDEIGSI
                     VAWLAGPDSGFTTGADFSCNGGLHMG"
     gene            complement(8528..9718)
                     /locus_tag="VLN25_02040"
     CDS             complement(8528..9718)
                     /locus_tag="VLN25_02040"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HSQ09385.1"
                     /translation="MTEAVIVAAARTAIGRFGGALAKVSAPELGAVVIKEVLRRANVT
                     PEQVDEVILGQVLTAGSGQNPARQASLKAGLPVSVPAMTINKVCGSGLKAVMLAAQAI
                     RNGDAQIIIAGGQENMSASPHVMLGSRDGFRMGDAKIIDSMIVDGLWDVYNQYHMGVT
                     AENVAKAHGIDRGAQDALALASQTKAAAAQDAGRFKDEIVAVSIPQRKGDPVIFDADE
                     FINRKSNAETLAGLKPAFDKQGSVTAGNASGINDGAAALLVMSADKAKALGLTPLARI
                     VSFASVGVDPALMGMGPAPASRKALEKAGWKAEQLDLMELNEAFAAQACAVNKDLGWD
                     KAPFKDKINVNGGAIALGHPIGASGARVLVSLLHEMKRRDSKKGLAALCIGGGMGVAL
                     AVER"
     gene            complement(9786..11606)
                     /gene="phaC"
                     /locus_tag="VLN25_02045"
     CDS             complement(9786..11606)
                     /gene="phaC"
                     /locus_tag="VLN25_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HSQ09386.1"
                     /translation="MPRTKAPSTVRAAGRTPQRSAGGGKTAVKRRTKAALAPRAAEPV
                     KARPGTSTTLGATEPFNFALPWLQPSAAPTAMPLMPERLMEMQQDYVQRLVGLWNDFF
                     THPEKTTAPIADARFSDPTWQKNSLASFYARTYLLNSEFMNRLAESVQGDQKTRKRVK
                     FAVSQWVDAASPANFLALNPKAQQTLLDTSGESLKAGLGNLLNDIGKGKISMTDESAF
                     EVGRNVATSEGRVVFTNKLFELIQYKPLTEKVYTRPFLMVPPCINKFYILDLQPENSL
                     IRYAVEQGHTVFVVSWKNPHKAEAHITWDDYLETGVITAIHTVQELTGSKQINALGFC
                     VGGTLIANALAVMYARGEQPITSLTMLTALLDFEDAGVLDVFIDEAQVRLREQQFARG
                     GIMPGLELGKTFSSLRPNDLVWNYVVSNYLEGKSPPAFDLLYWNGDSTSLPGPMYAWY
                     LRNMYLENNLRVPGKLHCCGQSIDLGRIQVPTFVFAAKEDHIVPWRAAYASARTLPGG
                     GKAGGVRYVLGASGHIAGTINPPSKNKRNYWAGADDTLPANSDAWFNSAVEHPGSWWP
                     DWSRWLARYSGPLKPAPKQYGNAKYKPTVPAPGAYVKEKA"
     gene            complement(11736..12521)
                     /gene="pgeF"
                     /locus_tag="VLN25_02050"
     CDS             complement(11736..12521)
                     /gene="pgeF"
                     /locus_tag="VLN25_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HSQ09387.1"
                     /translation="MTMPPEWIRPDWPVHDAVRAVITTRSGGVSAAPYSGPGDSGGMN
                     LGYGSGDAAATVTANRARLRELLPAEPSWLKQVHGARVADAADVTGTVEADAAIVNRP
                     GAVAVVLMADCMPVLLADELGRCVGVAHAGWRGLAAGVIQATVRAMRERMGDSAARLR
                     AYLGPAIGPDHFEVGRDVFDAMTSRLDGAAGAFVDRGEGKYRADLFALGRLALAQERV
                     DAVYGGGVCTACDASHYYSHRRDRVTGRHAALIWLDGGGAAQP"
     gene            complement(12521..>13405)
                     /locus_tag="VLN25_02055"
     CDS             complement(12521..>13405)
                     /locus_tag="VLN25_02055"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HSQ09388.1"
                     /translation="SRLKQWIEVGAVRVNGATVRPRHAVVAGDHVELEPQPAPDAGAY
                     TPENMALAIVYEDDAILIVDKPAGLVVHPAAGHWSGTLLNGLLAYAPELSAVPRAGIV
                     HRLDADTTGLMVVARTLAAQTDLVRQLQARTVQREYWAIVLGEPRRTGVIDASIGRDA
                     RNPLRFRVSHATNARPARTHYTRLASVQADGATLSWLACRLETGRTHQIRVHLEYLGH
                     PLVGDPVYRRRLPSLAPEIAWRCFARQALHACRLGLDHPTTRAPMQWFRTPPADLAGL
                     MLSLGFGPVDRPMAMESA"
BASE COUNT         2300 a         4422 c         4267 g         2416 t
ORIGIN      
        1 tcatcggcga ctgcatgttg acgaactgtg tccacatctc aggcccgatc ttgttcgtct
       61 cgtagaaggc cttgctttgt tccgacagct tgccctggat gtcgatgaac gcctgtatgt
      121 tcttttccag gtacgagccc atcatgccct gcatcgcgtt gccgtagaaa cggatgatct
      181 gcgacagcat cgtcgcgctg aacatcggca tgccgccggc ctcttcctcc aggatgatct
      241 gcagcaggat cggtcgggtt aggtcctccg cgctcttcgc gtccacgacc tggaagttct
      301 cgttgtccaa caccagctgc ttgacatccg ccagcgtgat gtaggtgctg gtctgggtgt
      361 cgtacagacg tcggttcgga tacttcttga tcagtcgaac aggtgttgcc attgatggtc
      421 ctcgattgtc ttcgcgcagt gcgtgcgcat cggcccgtcg ttgcgggcgt ctcccttgaa
      481 tgtcgcgcga ttctaacgaa tttgacgcag cgcagcggac gacggccccg gatcgatagg
      541 ggtagcccgg gcacggtcgt atttgccaga atcaaagcga gtttctcggc gagttcacgc
      601 gctatcgcgt gcgtgtcggc acgaccctgc tgacggccga cgctccgcac ctgtcggcga
      661 caccaatcta ctcgccgggc atgggcgtgc agatcggaat cgatccggat gaggtccggg
      721 ttctcgcctg aacgtttccg gcaaaggggc gcatcgatcg gatcagtcga cgtcggcgcg
      781 ccgcccggtc agcttctgcg gtcctgatcg gcgtactgcc ggctgtccag cgcagcggag
      841 tccttcggtt cttcggcccg ttccgcacgc cgtttgactg cgagattcat cgccgcgagg
      901 gccggttcga tgatttgctg caggcgatgt cgcgcaaagg gaagcgcgag gccgcggtac
      961 tgctcgcagt tgtggagtcg caccccgccg ccgggcagcg actcgatgct atagcggtgc
     1021 tccgccgaat acagcccggg aaccgcccag cgaccgaacc agcgcagctc gcgcggttcg
     1081 cgcacatgag tgacggtagc gcgaaacgtc gatgcgccga aggccacgac gtcctgcgtg
     1141 atctcgatcg agttgccgac ctgtgactga cccgtcaccg accgcatgta aggattccag
     1201 cggcgatagg acccgaagtc ggtcaggatt ccccaaacga gcgacgccga agcggcgata
     1261 tcgatttgcg tgcggatttc gagcattgcg cagtatggga cccgaccgca ggtccgatcc
     1321 cgtgaagtac agagcggacc gggggtgaac gtaaatgcgg ggtactggcg tcgtcccggc
     1381 ggccatccgg tgcagccgac tggcgagatt tgaagccagt cagcgtgcga agtccttcgg
     1441 actgtaccgg gccgatgacc ggcaaggcaa tgccagaatg ggccattttc cccaccacaa
     1501 gtgactgagc gacacgctga catgcgccgg cgaatcattc tgggttccgc aatcggtgtc
     1561 gccttcatcg ctcctgcgcc gacgctcgcg cgcacgccgg cagtgcttca tcgtccagtc
     1621 cagatcctgc tcccgtttcc cagcgacgtg gcctactcgc tggcgcgact gatcgccgac
     1681 ggcatcagcc aggccgtcgg gcagcctgtt tttatcgaaa cccggtccgg cgcgaccggg
     1741 cgtgtggccg gcgaggtact gaagaatgcc gcaccggacg gcacgacact cgcgttcttc
     1801 cccatcgcag tgccggtgct cgcacccctg gtcttccggc agttgcgttt cgacccgccg
     1861 cgcgattttc tggcggtcac cgaaatcgct cgcttccact acgcgctggc ggtggcagcc
     1921 gaccatccgg cgcgctccgt gcccgagttc atcgcgtggt cgcgacagca tccggctcgg
     1981 gccagtgtcg gcacctccgg cgccggaacg atttctcact tcttcacggt catgatcaat
     2041 cgggccgcca aagccgagct gctgcacgtg ccctaccgga atgtcggcgt gatggccacg
     2101 gatgtcattg gcggccagat cgccggcggg atcgatgcgc tggccacact gatcgaattt
     2161 caccgggcgg gccgcctgag gatcatcgcc actacccatg tgacccgctc ggcgctgctg
     2221 ccagccgtgc cgaccttcgc cgagcagggc ctgccgggag tccagggcgt cggctggatc
     2281 ggcgtcttcg tgccggccgg aacaccgcgc ccgatcatcg acatcttgtc gagggcaatc
     2341 ctgcaggtct tgcgaactcc gcaggtccgg gaccagttcg ccgcgctcgc cgtcgaagtc
     2401 accggcacat cgcccgagga attcgcggcg atcatcgcca ccgataccgc ccactgggcg
     2461 ccgatcgtca gggcttccgg attttcaggc gattagggat gcccacggcc gcgcctcgtg
     2521 cgtctttgtc caccgcgcat tcctcggccg gcgctgaagg ccgcgaccgg tctgccccgt
     2581 gacgatcggt cagcgtaccg aggaacacga gcaggtcctc gacctcggtg tcgttcagct
     2641 tcagggcacg caacaggttt tcgccgtcgg catgcaggcg ctcttggtcc agttccgagt
     2701 agtgcctgat gacgtcttgc agcgtgtcga ggctgccgtt gtgcatgtac ggcgccgtca
     2761 cggcaacatt gcgcagcgac ggtgtgcgga actcgcccca gttgcgcgac gagagcacga
     2821 cgttacgcgt cggcgtcgtg ttggtgcgcg tcgtgtcgtc attgaacctc gacaacagat
     2881 tgtacgggtg ggtcttcaca gcctcgatgc cctcgaagcg cccgccgtcg gcccggccgc
     2941 gaccgatgaa atgacccacg ccgttgacat ggaattcgcc gttgctgaag ttgggtccgt
     3001 tatggcacag cacgcagttg ccgcggccga tgaaaagctg cagcccgcgc accgcggcgg
     3061 cggggtatgc ggccgccgtt gcaccgtcac cgcgggcgag tgcatcgcgg tagtcgtcga
     3121 acggtgttcg tccggtgatg agcgtttcct gaaaggccgc aagcgccttg ccactgttga
     3181 ccagtacggt cacatcatcg tcgcgcgtgt catccgcagg atggccgaag gcacgcccgt
     3241 accagcacgc cagttccggc tcacgcacat agagatgtcg gatgtgtgcc ggcgtggaat
     3301 cgagctcgcg ttcgtcaagg atcggccgca ggctggccat ccacaggctg tcactggctc
     3361 cgctccatcc ataccagcgt gccagccgga ggttcgccag catgggcgtg ttgcggtcaa
     3421 cgtctgccag cccgtgcgcc cgggccttgg cgtcgcccca ggcacgatcc ggctgatggc
     3481 aagtcacgca agcgatgtag ccgacgcggg acaggcggga gtcgaagaaa aggcgccgtc
     3541 caagttccac ggccgccggg ttgcccgaca cgcgattgcc cgcatccttc gtcgccgcgg
     3601 gcggccacgg cccgaacgac agcacgcgct cgatctcctc gggcgacagt tgcgcctgcg
     3661 tcggcgagcc cgctcctagc atcagcgcgc tgaagaacca cgacactgca cggaatcgaa
     3721 gctgcatggc cggcacctga ccgcgtcact tcagttcgac aatcgatcgg accgtctcgc
     3781 gcgtgctgtc gccggcaagg tcgaacgtga tttcccagcg tcccggcatg tgcagcagca
     3841 acccgctcgc ccgaaaacgg cctcctgctt cggacgtcac ggtgggcgca tagttcattc
     3901 cgtgccggtg tgccggcatg gtggcgtcaa ccttcaccga ggacaccggc tggcccgccc
     3961 gctcgcacgt actgacgacg accgtgaaga actcgctcaa ccgcagcggc gcgggttcca
     4021 cccgccagtc gacaacaaag atgcggcccg tgatacgcgc cgcatccgcg ccgatcggag
     4081 ccggctcgca gccgattgcg gccccgaaag cgctggacag cgccgcggtg accactgcgc
     4141 cccgtcgccc ccaagccgtc atttgccaag cgcccctgca aacagcgcct cgccgttccg
     4201 gtatgcgatt cgctcggcca catcgggcgg cagatcggaa agccaggcac gcgaccagcg
     4261 cgcgtgctcg acgatgaacg gccagcgttc gggggtgaac gtgtcggtgc cgaccatgaa
     4321 ccggtccgga aactcgagca tcagcgcccg ccagtcggga tcgagcttgc cgcccgcgcc
     4381 gtgctcgctg cgaaaagcca ggtcgcacca caggttgcga tgtttcctga gcatggcgcg
     4441 gatatcagcc ggcgagacga agccggcatg tgcccagagg atgcgcgcct ggggccactg
     4501 catgaattgc cgttcgattg cgtcggcatc cgagtgtgaa tgcagcacaa ggtcgtgttt
     4561 gcgggcgagt tcgatcatgc gccgcggtac ggccaggtcc acgtcggcgc cgtagatatg
     4621 gaactcgccg acggcgacgt accggtactt cgccaggcgc tcctccaggt agtggatgac
     4681 ggacggatcg cgcacccacg ttgagatttc gccacgacga cgatagggac gcagcgacgg
     4741 aaggaagacg tcgggcgcca gccggaccag tcgttgctga ccgtcgtcat cggagcttga
     4801 aacgagcgca cgcttgacgc cggccttgcg caccaggtcg agagcaacct cgggcggcag
     4861 catctcccac gcgtcatggc tgtagtgcac gtgggcatcg aagatcggca ggctctccgc
     4921 agactccccg gccacaggca tggcgagact ggcgacggcg gtggcagcca tcaccgagag
     4981 caggaggggt ctggtcaaag ggccatccag ggaaacgctc gattcgacga agcgtcagcg
     5041 aaaggtgccg gacccgtgcg gcgccgccat gaaggccggc agttcctcgg cacgttgaga
     5101 aaccagttgc agattagggt ggtttgcagc gagcaccagt tcaggcagcg tctgctgtgt
     5161 gaagtgccag gcgacggccg tcgagatacc cgcctgatcg atcgtactgg tcgacaccgg
     5221 gagggggcgc gcccgcaatt caacttcaag cgcctcgtag gcagcacgta gttgtcccgt
     5281 gacccgtgca atccacggct cgtgccactt ctcttccggc cgcagactgc gttcataaat
     5341 gatctgcacg ctcttctcgc aggcaacaag cgccaggccg atcaggcgga gcgcatgctg
     5401 gcgggcagcg acgtccgtcg gcatgagact gcgccgggga gccgagagcg cttccgcgta
     5461 ctcaagaatc agtgacgaat ccatcagcac ttcgccgtca tcgcagacca gcgatggcgc
     5521 cttgacgacg gggttgatct tccggaagtc ctcgaaactg cggaagaccg agacggaccg
     5581 gtgttcgaac tgcagaccaa gcagttgaag tgaaatcgcc acgcggcgca cgtacggcga
     5641 gtcgagcatg ccgatcagta ccaaggtctt cctccggagt gatcagagcg cgcccgcgcg
     5701 cgctgcgtcg agtttcccga cggtaaccgc cgcccgggcc gctgacaatc tctgcaacac
     5761 gccggtttcg gaccctggca ctgctcgcgt cttcgcggga tgcgcctgct tgctcgcccg
     5821 tcgcctgatt gcggtgaccc tgtcagctac ctagactgca ctcgttgcac gcatcgcgtg
     5881 tgaccataga gaaccactgg gaagacaagg gagaagcgca tgaatccggt ttggaaggcg
     5941 actgccattg cggttgcgtt ggcggtcggc agcgtcgcca acgtgagtgc tcagcagaat
     6001 atcgagaagc tcaagcagat gagggcctcg ggtacggacc cgaacatccc gtccatccca
     6061 cagtcgggca agaacgccga tgcgctgcgc gagaacctga agaaggtcaa gttgccgccc
     6121 ggcttcaaga tcgaactctt tgccatcgtg cccgacgcgc gtcatatggc gatcgcgcca
     6181 agcacaaaca tgctgttcgt cggcacgcgc aagacggcag tgtgggcggt gaccgatcgc
     6241 aacagcgact atgtggccga cgaggtcaag caattcgctc cctcgctgaa attcagcgtt
     6301 cccaacggcg tgtgctggac caaggacggc ttcctgatcg tggtcgaaca caaccgggtg
     6361 ctcaacttcc cggccgccga gttcttctac gaaggtcccg acgtcgcggt gatcgaggtg
     6421 gtcccgcagg gcaaactcat tccggaggag gaagagtcgt acaaccacca cgcgcgcacc
     6481 tgtcgcgtcg ggcccgacgg catgctctac atcacgctcg ggcagccgtt caatgtgcag
     6541 ccgcgcgaca agatcgcgat cttcaacaag gtcggcatcg gcggcatcgt gcgcatgaat
     6601 ccgttcgacg gcagcaagcg cgaggtctac gcgaccggaa tccgcaattc ggtaggaatc
     6661 gacatcagtc cgaaggacgg ctcggtctgg ttcaccgaca accagaccga cggcatgggc
     6721 gatgacatcc ccgcgggcga actgaaccgc gccaccaagc cgggccagtt ctttggctat
     6781 ccgtggaaac agggcaagac gcggatcacc gagcacggct acgacaagga cccgctaccc
     6841 gccaacatca ccgacccgca ggtgtatttc gatgcgcacg cggccgacct gggcctcacg
     6901 cactacaccg gcaaacagtt tccggcgaag taccaaggcg gcattttctc ggcacagcac
     6961 ggttcctgga atcgcaccac gccgattggc gcgcgcgtga tgttcacgtc gctcaagccc
     7021 gatggcacgg ccgacaagac ggagttgttc gccgacggct ggcttgacaa caccacggga
     7081 acctaccgcg ggcgtccagt cgacgtcgcg gtgatgaagg acggttcgtt gctggtgtcg
     7141 gacgacttcg cgggcgcgat ttatcgcatc tcgtaccagc cctgagcgca tgcctcgtcg
     7201 ttatcggacc gccgtcgcag tacttgcgac ggcggtatcg tcattgctca gcagtgcaca
     7261 cgacgctgct gcgcaggacg tcacggccgg gcgccgcaag gcgattgctt gccagaactg
     7321 tcacggtatg gacggcctcg ctaagttgcc ggatgcgcca catctggcgg gccagcctgc
     7381 tgcttacatg gtccgctcgt tgcgggccta ccgctcggga gagcgtcgca acgaggtgat
     7441 gtagatcgcg gccaagacgt tgactgacga agacatccgc gatctggccg cttactacgc
     7501 cgcgatacaa atcgaagtga agtcgattcc gcagtaaggc ggaatccctg gcagacgtcg
     7561 acgcgggcag gaaggctgca gtctttatcg tccagcagcg agtgttcccc tcaccgcggg
     7621 ggcgaacgca cacaggtgcg ttcgccgcat gcccgctagc actaccccat gtgcaggccg
     7681 ccgttgcagg aaaagtcggc accggtggtg aatcccgaat ccggaccggc cagccaggcg
     7741 acgatcgaac cgatttcgtc cggcgtaccc agccgcttga ccgggatcgt cgcgacaatc
     7801 ttctcgagta cctcgggctt gatggcgttg accatctcgg tgccgatgta tccggggctt
     7861 accgtattga cggtgacgcc cttcgaggcg acttcctgtg ccagcgccat ggtgaatccg
     7921 tgcatgccgg cttttgcagc cgagtaattg gtctggcccg cctgtccctt ttcgccgttg
     7981 acggaactga tctggatgat ccggccccat cctttctcga ccatgcccgg cacgacctgc
     8041 ttggtgacgt tgaacatgct gttgagattg gtgtcgatga cggccttcca gtcctcgggc
     8101 gtcattttca ggaacatgcg gtcgcgggtg atgcccgcgt tgttgaccag cacctcgatc
     8161 ggtccgtgct cggccacggc ctttgagaac gcggagacgg tcgagtccca gtcggccacg
     8221 ttgcccacgg atgcataaaa cgtaaggccg agcgccttct gttcaccgag ccatttgccg
     8281 aaatcgcgac tcggtccgca gccggcgatg accttgaagc cttccttgtg cagacgctgg
     8341 cagatcgccg tgccaatgcc gcccatgccg cccgttacat atgccacctt tgccatgtct
     8401 gtctccgcat gttgttaact gctgttgtgt tcgggtcggg cgccgggtcg ccccaagccc
     8461 gacccgcaaa atggtctcgg ccgagtgcac atgcgacgcg cagcgtcgcg tacacgaggc
     8521 cgaccggcta tcgttcgacc gctagtgcta cacccatccc accgccgata cagagcgcgg
     8581 cgaggccctt cttcgaatcg cggcgcttca tctcgtgcag caggctcacc agaacgcgag
     8641 cgcctgaggc gccgattggg tggcccagtg caatcgcgcc gccattgacg ttgatcttgt
     8701 ccttgaaggg agccttgtcc cagccgagat ctttgttaac agcgcaagcc tgggctgcga
     8761 acgcttcatt gagctccatc aggtcaagct gttcggcctt ccagccggct ttctcgagcg
     8821 ccttgcgcga cgcaggcgcc gggcccatgc ccatcagcgc cggatcgacg cctaccgatg
     8881 cgaagctgac gatgcgcgcc agcggcgtga gcccgagcgc cttcgccttg tcggccgaca
     8941 tcaccagcaa cgcagccgct ccgtcgttga tgccggacgc attgccggcg gtgaccgacc
     9001 cctgcttgtc gaatgcgggc ttgagcccag cgagcgtttc ggcgttcgac ttgcggttga
     9061 tgaactcgtc cgcatcgaag atcaccggat cgcccttgcg ttgcgggatc gagaccgcaa
     9121 cgatctcgtc cttgaagcgc ccggcgtcct gagcggcggc ggccttggtc tgcgacgcca
     9181 gtgccaacgc atcctgggcc ccgcgatcga tgccgtgcgc cttggcgacg ttctccgcgg
     9241 tgacgcccat gtggtactgg ttgtacacgt cccacaggcc gtcgacgatc atgctgtcga
     9301 tgatcttcgc gtcgcccatg cggaagccgt cgcgtgagcc gagcatcacg tgcggcgacg
     9361 cgctcatgtt ctcctgcccg cccgcaatga tgatctgcgc atcgccgttg cggatcgcct
     9421 gggccgcaag catcaccgcc ttcagccccg aaccgcacac cttgttgatc gtcatcgccg
     9481 gcacgctgac cggcagtccc gccttcagag atgcctgacg cgccggattc tggccgctgc
     9541 ctgcggtcag cacctgaccc aggatgactt cgtcgacctg ttccggcgtc acgttcgcgc
     9601 ggcgaagaac ttccttgatg acgacggccc cgagttccgg ggcggagacc ttggcgagcg
     9661 ctccgccaaa ccggccgatc gcagtccgtg cggccgcgac aattactgct tcagtcatga
     9721 catgactccg atggagtagt gaaacgggcg gaacctgacc cgcgcgtgcg gatcgaactg
     9781 caaggctatg ctttttcctt gacgtaggcc ccgggcgcgg ggacggtcgg cttgtacttg
     9841 gcgttgccgt actgcttcgg cgccggcttg agcggcccgg aataccgcgc cagccagcgg
     9901 ctccagtccg gccaccagct gcccgggtgc tctaccgcgc tgttgaacca ggcatccgag
     9961 ttggccggca gcgtgtcgtc ggcgccggcc cagtaattgc gcttgttctt cgacggtgga
    10021 ttgatcgtgc cggcgatgtg cccgctggcg ccaagcacat agcgaacgcc gcccgccttg
    10081 ccgccacccg gcagcgtacg cgccgaggca taggccgccc gccacggcac gatgtggtcc
    10141 tcctttgccg cgaacacgaa cgtcggcacc tggatgcggc ccaggtcgat cgactggccg
    10201 cagcagtgca acttgcccgg cacgcgcagg ttattttcga ggtacatatt gcgcaggtac
    10261 caggcgtaca tcggcccagg cagactggtg ctgtcgccgt tccagtaaag caaatcgaag
    10321 gcgggcggcg acttgccctc gaggtagttg ctgacgacgt aattccagac caggtcgttg
    10381 ggccgcaggc tcgagaacgt tttcccgagt tcgagccccg gcatgattcc accgcgtgcg
    10441 aactgctgct cgcgcagacg aacctgagcc tcgtcgatga acacatcgag cacgcccgca
    10501 tcctcgaagt cgagcagcgc ggtgagcatc gtgaggctcg tgatcggctg ttcgccgcgc
    10561 gcgtacatca cggccagtgc attcgcaatc agcgtgccgc cgacgcagaa gccgagcgca
    10621 ttgatctgct tgcttccggt gagctcctgg acggtgtgga tcgccgtgat cactcccgtt
    10681 tcgaggtagt cgtcccacgt gatatgcgcc tcggccttat gcgggttctt ccacgacacc
    10741 acgaacaccg tatgtccctg ctcgaccgcg tagcggatca aggagttctc gggctggagg
    10801 tcgaggatgt agaacttgtt gatgcacggt ggcaccatca ggaatggcct cgtgtagacc
    10861 ttctcggtca ggggtttgta ctggatcagc tcgaacagct tgttcgtgaa gacgacccgc
    10921 ccctcgctgg tcgcgacgtt gcgcccaacc tcgaaggcgc tctcgtcggt catggagatc
    10981 ttgcccttgc cgatgtcatt cagcaggttg cccagcccgg ccttcaggct ttcgccgctg
    11041 gtatcgagca gggtctgctg cgccttcgga ttcaacgcga ggaaatttgc cggcgacgcc
    11101 gcgtcgaccc actgtgacac cgcgaacttc actcgcttgc gtgtcttctg gtcgccctgc
    11161 acgctttcgg cgaggcgatt catgaactcc gaattgagca ggtaggttcg cgcgtagaac
    11221 gaggcgagcg agttcttctg ccacgtcgga tccgagaacc gtgcgtcggc aatcggcgcc
    11281 gtcgtctttt ccggatgggt gaaaaagtcg ttccacagcc cgacgagacg ctgcacgtag
    11341 tcctgctgca tctccatcag gcgctctggc atcagcggca tcgcagtggg cgcggccgac
    11401 ggctgcagcc acggcagcgc gaagttgaac ggctcggtgg cgcccagcgt cgtcgatgtg
    11461 ccgggtctcg cctttaccgg ttctgcggca cgcggcgcca gtgctgcttt cgttcgacgt
    11521 ttgaccgccg tcttgccgcc tcctgcactg cgttgcggcg tccgtccggc agcacgcacc
    11581 gtactgggag ccttcgtcct gggcatgtct cctccgattg cctggccggt ggatcgaacg
    11641 atccgtgcgg ctctgtggtg gtccagcgat tctccacatc gcgcagagca cggtcaaacg
    11701 gcgtcacggc ccgcttgacc tgcatcagcg taacgtcatg gctgcgcagc gccgccgccg
    11761 tcgagccaga tcagtgcggc atgccgccct gtcacgcggt cccggcgatg actgtagtag
    11821 tggctggcgt cgcaggccgt gcagactccg ccgccataga ccgcgtcgac acgctcctgt
    11881 gccagtgcga gccggccgag cgcgaacagg tcggcacggt actttccctc cccccggtcg
    11941 acgaaggcgc ccgccgcgcc atcgagccgg ctcgtcatcg catcgaacac gtcgcgtccg
    12001 acctcgaagt gatccggccc gatggcgggg ccgaggtagg cccgcaggcg ggccgccgag
    12061 tcgcccatcc tttcgcgcat cgcccgcacc gtcgcctgga tcaccccggc cgcgagcccg
    12121 cgccagccgg catgcgccac cccgacacag cggccaagtt catcggccag cagcacgggc
    12181 atgcagtcgg ccatcagcac gaccgccacg gcgccgggcc ggttgactat ggcggcgtcc
    12241 gcttcgaccg tgcccgtcac atcggccgca tccgccaccc gcgcgccatg gacctgtttc
    12301 agccagctcg gttcggcagg aaggagttcg cgcagtcgag cccgattggc cgtcacggtc
    12361 gctgccgcat cgccggagcc atagccgaga ttcatgccgc cggaatcgcc cggtccactg
    12421 tagggcgcag ccgagacgcc gccggagcgt gtggtgatta cggcgcgaac ggcgtcgtgc
    12481 acgggccagt ctgggcggat ccattcggga ggcatcgtca tcacgccgac tccatcgcca
    12541 tcggccggtc gaccggaccg aagccgagcg aaagcatcag tccggcgagg tcggccggag
    12601 gggtccggaa ccactgcatc ggcgcgcgcg tggtcggatg atcgaggccc aggcggcagg
    12661 catgcagcgc ctgtcgcgca aagcatcgcc aggcgatctc gggcgctagt gatggcaggc
    12721 ggcgccggta cacgggatca cccaccagcg gatgaccaag gtactcgaga tgcacgcgga
    12781 tctggtgcgt gcgcccggtc tcgaggcggc acgcaagcca cgacagggtg gcaccgtccg
    12841 cctgcacgct ggccagtcgt gtgtaatgcg tgcgcgccgg tcgcgcgttg gtcgcatgac
    12901 tgacccggaa gcgcagcgga ttgcgcgcat cgcggccgat cgaagcatcg atgactccgg
    12961 tgcgccgggg ttcgccgagc acgattgccc aatattcgcg ctgcaccgtg cgggcctgca
    13021 actggcgcac gagatcggtc tgggccgcca gcgtccgcgc cacgaccatc aacccggtcg
    13081 tatcggcgtc gagtcggtgg acgatgccgg cgcgcggcac ggcggaaagc tcgggtgcgt
    13141 acgccagcag gccgttgagc agcgtgcctg accagtggcc cgcggccggg tgcacgacca
    13201 ggccggcagg cttgtcgacg atcaggatcg cgtcgtcttc gtagacgatc gccagcgcca
    13261 tgttctcggg cgtgtaggcc cccgcgtcgg gcgccggctg cggctcgagt tcgacgtggt
    13321 cgcccgcgac gacggcatgg cgcggtcgga ccgtcgcgcc gttaacccgc acggccccga
    13381 cctcgatcca ttgcttgagc cgcga
//
LOCUS       DATIRT010000079         1677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1680741, whole genome shotgun sequence.
ACCESSION   DATIRT010000079 DATIRT010000000
VERSION     DATIRT010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680741"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..612
                     /locus_tag="VLN25_02060"
     CDS             118..612
                     /locus_tag="VLN25_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone"
                     /protein_id="HSQ09389.1"
                     /translation="MATKKAARKRAVKKAATKAKAKKAVKKVKKAVKKAKVKRAVKKA
                     ATKAKVKRAVKKTKAKRAVKKAKVKRAGKKVATKAKAKRAVKKVKKAVTKVKVKKAVA
                     KVKAKVAKAVTAVKAAVVPKPAAKPMAAPAPVPAAPSTAAAPGAKTALNPAAAWPFPT
                     GSRP"
     gene            712..1281
                     /locus_tag="VLN25_02065"
     CDS             712..1281
                     /locus_tag="VLN25_02065"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HSQ09390.1"
                     /translation="MRAFHLIALPLLFIDIARALLDILGSLLVGLLLLRAWAAAIGMP
                     ARNPLAHFARALTDWLVKPLARVIPSRGRVEWTALLAALVATVVVVAVKVAVSGMSIA
                     WDLVLVESVRQLLHWALTLVIWVTLIYVVISWVNPLAPVAPALSMLLRPLLEPIQRIL
                     PAIGGIDLSPMALLIVVYLLQMIISRIGI"
     gene            complement(1347..>1677)
                     /locus_tag="VLN25_02070"
     CDS             complement(1347..>1677)
                     /locus_tag="VLN25_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HSQ09391.1"
                     /translation="RLSLPELAQRVKALVVTRGAEGSEIFLGGTSLRVPVSSARRVVD
                     PIGCGDAYRGGLLYGIAKGLDWETTGRLAAVLGAIKIEASGAQNYVSDRQAIADRFSE
                     AFGYRPW"
BASE COUNT          323 a          534 c          535 g          285 t
ORIGIN      
        1 ggctgccttc gggcagccgg tgtggcattc cagcccgcgt tcgaggcatc gattcgcgct
       61 cggaccggaa tgcgttgtcg ctaggcactt cacgccattc tcgttcagga gactgacatg
      121 gcaacgaaga aagctgccag gaagcgtgct gtaaagaagg ccgcgaccaa ggcgaaggct
      181 aaaaaagccg tcaagaaagt caagaaggcg gtcaagaaag ccaaggtcaa gcgcgcagta
      241 aagaaggccg cgaccaaggc taaagtcaag cgggctgtaa agaagacgaa agccaagcgc
      301 gcagtaaaga aagcgaaggt aaagcgtgca ggcaagaagg tcgcgaccaa agccaaggcc
      361 aagcgtgccg tcaagaaggt gaaaaaggcg gtgacaaagg tcaaggtgaa aaaggccgtt
      421 gcgaaggtca aggcgaaagt tgcgaaggcg gtgacggccg tgaaggcggc ggtcgttccg
      481 aagcctgcgg ccaagccgat ggcagctccc gcaccggtac ccgccgcacc gtcgacggct
      541 gcggctcccg gtgcaaagac cgcgctgaat ccagccgcgg cctggccgtt cccgaccggc
      601 agccgtccgt aggtctacgc agtactgaaa acgggcgcga attctcgcgc ccgttttttt
      661 tgaccgcgac gccagcggca acacggggtc gtcgcggcgc ccgccgctac aatgcgggcc
      721 ttccatctga tcgcactgcc cctgcttttc atcgacatcg cacgtgcgct gctggacatc
      781 ctgggttcgc tgctggtcgg cctgttgttg ttgcgcgcgt gggccgcagc gatcggcatg
      841 ccggcgcgca acccgctggc gcactttgct cgcgccttga ccgactggct ggtgaagcct
      901 ctggcgcgag tgattccgtc gcgcggccgc gtcgaatgga cagcattgct ggccgcactg
      961 gtcgcgaccg tcgtcgtcgt cgctgtcaag gtggcggttt cagggatgtc catcgcatgg
     1021 gacctggttt tggtcgaatc ggtgcgacag ctactccatt gggccctgac actggtcatc
     1081 tgggtcacgc tcatttacgt cgtgatcagc tgggtgaacc cgctcgcccc ggtcgccccg
     1141 gcgctgtcga tgctgctgcg gcctctgctt gagcccatcc agcgcatcct gcccgccatc
     1201 ggagggatcg acctctcgcc gatggcgctg ctgatcgtcg tctacctgct gcagatgatc
     1261 atcagccgca tcggcatcta gcgctcgtta ctggatgcgc ttcgcatccg aggcagacgc
     1321 tcgcaggtag cgtcgcccgg cggagctcac cacggccgat agccgaacgc ctcgctgaag
     1381 cggtccgcga tcgcctggcg atcggaaaca tagttctgcg cgccactggc ctcgatcttg
     1441 atcgcgccga gcacggcggc aagccggccc gtggtttccc agtccagacc cttcgcaatg
     1501 ccatagagca atccgcctcg ataggcatcg ccgcagccga tcggatcgac aacgcggcgc
     1561 gcgctcgaaa ccggcacgcg cagtgaggtg ccacccagga agatttccga gccttcggcg
     1621 ccgcgcgtca ccaccagtgc cttcacgcgc tgcgccagct ccggcagtga cagtcgc
//
LOCUS       DATIRT010000080         1968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1189498, whole genome shotgun sequence.
ACCESSION   DATIRT010000080 DATIRT010000000
VERSION     DATIRT010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189498"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VLN25_02075"
     CDS             complement(<1..489)
                     /locus_tag="VLN25_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HSQ09392.1"
                     /translation="MPTAAGPNVVSLIGAPTDIGASVRGASMGPEALRVAQIKPALER
                     HGLEVIDHGNLNGPPNPWLPPVDGYRHLDEVVAWNRLVHEAVAAELAGGRLPILLGGD
                     HCLAIGSISAVARHCSERGKRLRLLWLDAHADFNTNALTPSGNVHGMPVACLCGHGPR
                     PLI"
     gene            complement(620..1522)
                     /locus_tag="VLN25_02080"
     CDS             complement(620..1522)
                     /locus_tag="VLN25_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HSQ09393.1"
                     /translation="MSDTEKFETPATPATTDSQGMKYLESTPRRLVTVYLPLAVFLIV
                     LLFPFYWMTITAFKPNEELLSREGNPFWVINPTLAHIKKLLFDTAYPDWLWNTILVSV
                     VSTLLSLFCSVLAAYAIERLRFRGSRYVGMSIFLAYLVPPSILFIPLAAIIFKFQLFD
                     TRLALILTYPTFLIPFCTWLLMGYFRSIPFELEECALIDGASRLQILVKIILPLAVPG
                     LISAGIFAFTLSWNEFIYALTFVSSSEVKTVPVGVITELVEGDVYHWGALMAGALFGS
                     LPVAILYSFFVEHYVSGMTGAVKE"
     gene            complement(1695..>1968)
                     /locus_tag="VLN25_02085"
     CDS             complement(1695..>1968)
                     /locus_tag="VLN25_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HSQ09394.1"
                     /translation="LLTPIIAVVMTFSVLFTFTDFQLIYVLTRGGPLNSTHLMATLSF
                     QRAIPGGALGEGAALATAMVPFLMAAILFSYFGLQRRAWQQGGQDK"
BASE COUNT          382 a          642 c          642 g          302 t
ORIGIN      
        1 gatcagcggc cgcgggccgt ggccgcacag gcacgccacc ggcatgccgt gcacattgcc
       61 gctcggcgtc aatgcattgg tgttgaagtc cgcgtgggca tcgagccaga gaaggcggag
      121 gcgcttgccc cgctcgctgc agtggcgtgc gaccgcgctg atcgagccga ttgcaaggca
      181 gtggtcgcct ccgagcagga tcggcaggcg gccgcccgcc agctcggctg cgaccgcttc
      241 atgcacgagc cggttccacg cgaccacttc atcgagatgc cgatagccgt ccaccggcgg
      301 cagccacgga ttgggtggcc cgttgaggtt cccgtggtcg atgacctcga ggccatgccg
      361 ctccagtgcc ggcttgatct gcgcaacccg cagcgcctcg ggacccatgc tcgccccgcg
      421 cacgctggcg ccgatgtcgg tcggcgcgcc gatcagcgat acgacgttcg gtcctgcggc
      481 ggtgggcatg caagttgtcc tggtcggtcg tgagtggcag atcgcgggct ggatggcgtg
      541 gaacgcctgg gccgctcggc gcgctcaagc gtcaccggtc ttgagattag cagtcaccct
      601 cgggcagcgc cgggagacgt cactccttca ccgcacctgt catcccggat acgtagtgct
      661 cgacgaagaa ggagtacagg atggccaccg gtaatgagcc gaacagcgcg cccgccatca
      721 acgcgcccca gtgatacacg tccccttcga ccagttcggt gatcaccccg acaggaacgg
      781 tcttgacctc cgacgacgag acgaaggtca gtgcatagat gaactcgttc cacgacagcg
      841 tgaatgcaaa gatcccggcc gaaatcagcc ccggtaccgc cagcggcagg atgatcttca
      901 ccaggatctg caggcggctc gcgccgtcga tcagtgcaca ttcctcgagc tcgaacggga
      961 tcgagcggaa gtagcccatc agcagccagg tgcagaacgg aatcaggaac gtcgggtacg
     1021 tcaggatcag cgcgaggcgc gtgtcgaaga gctggaactt gaagatgatc gctgccagcg
     1081 gaatgaaaag aatggacggc ggcaccaggt acgccaggaa gatgctcatg ccgacatagc
     1141 gcgacccgcg gaacctcaac cgttcgatcg cgtaggcggc cagcacgctg cagaacagtg
     1201 acagcagcgt cgagacgacc gacacgagga tcgtgttcca aagccagtcc ggataggccg
     1261 tatcaaacag cagcttcttg atgtgggcca gcgtcggatt gatcacccag aacggattgc
     1321 cctcgcgcga cagcagttcc tcgttcggct tgaacgcggt gatcgtcatc cagtagaacg
     1381 gaaacagcag cacgatcagg aacacggcca gcggcaggta cacggtcacc aggcgccgcg
     1441 gcgtggactc gaggtacttc atgccctgcg aatcggtggt cgcgggcgtg gccggcgtct
     1501 cgaacttctc ggtgtcgctc atgttgcccc cttggcgccc aagcgggccg caaagccgcc
     1561 tgcggtgtcc aaaatgccac ccgcgcttgc aagcgcgggc gcggcggccg tacggcatgc
     1621 ggcagcgcat gccgtcgagt cggccgccgt ccccccgagg gggagcgagc gcctccggag
     1681 cggccgcgcg gcgctcattt atcctgcccg ccctgctgcc acgcccgccg ctgcaatccg
     1741 aagtagctga acaggatcgc cgccatcagg aacggcacca ttgccgtcgc cagcgccgcg
     1801 ccttcgccca aggctccccc cgggatcgcg cgctggaaac tgagggtggc catcaggtgg
     1861 gtcgaattca gcggcccgcc gcgcgtgagc acgtagatca actggaaatc ggtgaaggta
     1921 aacagcaccg aaaaggtcat cacgaccgcg atgatcggtg tcagcaac
//
LOCUS       DATIRT010000081         7534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1681187, whole genome shotgun sequence.
ACCESSION   DATIRT010000081 DATIRT010000000
VERSION     DATIRT010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7534
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1681187"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1824)
                     /locus_tag="VLN25_02090"
     CDS             complement(<1..1824)
                     /locus_tag="VLN25_02090"
                     /inference="COORDINATES: protein motif:HMM:NF013444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSQ09395.1"
                     /translation="MSKVLVINDEPLILRELLKGLNAAARSLDNPFGITFVGALTARE
                     GLALIEREGDIQIAIVDDKLYAVQDTRKRRDGHKESARNLQVSALKLVQRITSLRPEL
                     DIYILIEKEKEDQVVDALFAEAVDGYFYREERDYRGMYRILNAQIAERARTPFYDALK
                     AYVLAAKDAWHTPGHSSGDSMRGSPWVNEFYDFMGEHVFDADLSVSVKALDSLMEPTG
                     VIAEAQQMAAKAFGARRTFFATNGTSTANKVILQTLIAPGEKLLLDRNCHKSVHHGVV
                     LSGARPVYLDSSVNRKYGVFGPVPTKTIMKAIEDHPDAELLVLTSCTYDGLRYNLKPI
                     IDAAHRHNIKVLIDEAWYAHARFHPALRPTALEAGADYATQSAHKTLSALSQAAYIHV
                     NDPTFNEHIFRENFNMHTSTSPMYSMIASLDVSRKQSVLEGYKLLQRTLMLADELRDQ
                     INSTDVFRVLELDDLLPEEVRDDGIRLDTTKVTVDISSCGMTVDDLQNELYTRFNITV
                     EKSTFNTLTLLLTIGTTRSKCSRLYDALMRIAREKRAPQHLYRTPDLPPFTELVSLPR
                     DAYYATGELVPLLDENDQLNGKLAGRICCDQIVPYPPGIPVL"
     gene            2075..2410
                     /locus_tag="VLN25_02095"
     CDS             2075..2410
                     /locus_tag="VLN25_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HSQ09396.1"
                     /translation="MSEPPAPAASKPREFFIHGVTSTGKPFRPSDWAERLCGVMSCYR
                     PGGIASGRDAYIGYSPYVRPTLIGGVKCVVVDERLRELERMAFDFVMSFAHDNDLPVY
                     EGCSMPDAN"
     gene            complement(2525..2788)
                     /gene="rpsT"
                     /locus_tag="VLN25_02100"
     CDS             complement(2525..2788)
                     /gene="rpsT"
                     /locus_tag="VLN25_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HSQ09397.1"
                     /translation="MANSKQARKRARQSTARNAQNASQRSRVRTAVKSVRKAIAAGDK
                     KAALDVLGTATGVIDSIADKNVIHKNTAARNKSRLAAAIKSMD"
     gene            3061..4626
                     /gene="murJ"
                     /locus_tag="VLN25_02105"
     CDS             3061..4626
                     /gene="murJ"
                     /locus_tag="VLN25_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HSQ09398.1"
                     /translation="MNLFRALATVSSFTLLSRVTGLIRDMLISRLFGASAETDAFNVA
                     FRLPNLLRRLFGEGAFQQAFVPMLADVRSRGDDPQTTAFAGHVASALFWVLLMVSILG
                     VVGAPILVWLIASGLARDPATFDLATVMTRWMFPYILFMSLVALAAGVLNTYKRFAVP
                     AFTPVLLNLAFIGCALWLAPRLSQPIMALAFAVLIGGALQLGLQWAALRRAGLRLRIG
                     MPRTAFADSDVRRVAGLMLPAIFAVSVAQLSIIINTNIASHLTTGSVTWLAFADRLME
                     FPTALVGVALGTILLPSLSTAFAEKRADDYSRLLDWGLRLIFLLALPAALGLGLLAEG
                     IVSVLFQGREFTAHDVHQTSIAVIGYAVGLIGLIAVKILAPGFYAQKDIRTPVKIAVT
                     VLIATQIANLVLVPLFAHAGLALSVSLGACANAVALYVGLKRRGMYTARPGWGGFVLR
                     LAIALLVLGGGLWWAQRPIDWTALQTSPWLRAGLLGTMITVAAGLYFAVLLALGFRPR
                     DFRPEQGRGAAPS"
     gene            complement(4741..5247)
                     /locus_tag="VLN25_02110"
     CDS             complement(4741..5247)
                     /locus_tag="VLN25_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HSQ09399.1"
                     /translation="MTIKVGDKLPDGELQEFIEVETAGCSLGPNTFKVGDLVKGKKIA
                     VFGLPGAYTPTCSAKHVPGYIAMVDQLKSKGVDEIWCISVNDAFVMGAWGRDQHATDK
                     VRMMADGSADYTKKLGLELDLTARGMGVRCQRFSMLVENGVVKTLNIEAPGKFEVSDA
                     GTMLKQVG"
     gene            complement(5313..7085)
                     /gene="ggt"
                     /locus_tag="VLN25_02115"
     CDS             complement(5313..7085)
                     /gene="ggt"
                     /locus_tag="VLN25_02115"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HSQ09400.1"
                     /translation="MVRLLRPIACVAVALLAACATAPQRPSQPEPSSAFRAGLETATA
                     QRHMAASANPLATLAGLDILRAGGSAVDAAITIQLVLALVEPQSSGLGGGAFLLHWDG
                     KRVVAFDGRETAPMAVDERLFLEADGKPMAFYDAVVGGRSVGTPGAVRLLETAHRQYG
                     KLPWADLFEPAIRLSENGFPMSPRLNQLLAGEKYLKSDAAAAAYFYNADGSAKAVGTV
                     LRNPQLAATLRAIAAGGADAFYSGPIAADIVATVRSQKNAGLLAASDLAAYRVRQREP
                     VCTDYKRWRVCGMPPPSSGGIAVAQMLGIFTARNITVFPPDDAPALEPRVEAVHLFSE
                     AGRLAFADRASYVADSDFVSVNVAGLLDPAYLKQRAQLIGPRSLGTAQPGVPPGASVA
                     WAPDPSPLKAATSHVSVVDDHGNAVSMTTTIEDVFGARVMVRGFLLNNQLTDFSFLPQ
                     VDGKLVANRVQPGKRPRSSMAPTLVFERDTGTLVATLGSPGGSQIIGYVAKTLVGVLD
                     WNLDIQQAITLPNFGSRNGPTEVEKGRIGAAVINGLKARGHDVREIDMASGLQGIVRV
                     RLTDGRLGWAGGADPRREGVALGD"
     gene            complement(7113..>7534)
                     /locus_tag="VLN25_02120"
     CDS             complement(7113..>7534)
                     /locus_tag="VLN25_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006483642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSQ09401.1"
                     /translation="GTFNMIRLAADAMSKQEPTAEGERGVLVNTASVAAFDGQIGQAA
                     YSASKGGVVGMTLPIARDLSRSGIRCVTIAPGLFATPMLLGMPQDIQDSLGRQVPFPS
                     RLGKPDEYAALVRHIVENVMLNGETIRLDGAIRMQPK"
BASE COUNT         1195 a         2405 c         2484 g         1450 t
ORIGIN      
        1 cagcaccggg atgcccggcg ggtacgggac gatctggtcg cagcagatgc ggccggcgag
       61 cttgccgttc aactgatcgt tctcgtcgag cagcggtacg agctccccgg tggcgtaata
      121 ggcgtcacgc ggcaacgaga ccagctcggt gaatggcggc aggtccggcg tacggtacag
      181 gtgctgcgga gcccgcttct cgcgtgcaat gcgcatcagt gcgtcgtaca ggcgcgagca
      241 tttggagcgg gtcgtgccga tcgtcagcag cagcgtcagc gtgttgaagg tcgacttctc
      301 gaccgtgatg ttgaagcgcg tgtacagctc gttctgaagg tcgtcgacgg tcatgccaca
      361 cgacgagatg tcgaccgtga ccttggtcgt gtcgaggcgg atgccgtcgt ctcggacttc
      421 ctccggcaac aagtcgtcga gctcgagcac gcggaacacg tcggtcgagt tgatctgatc
      481 gcgtagctcg tcggccagca tcagtgtccg ctgcagcagc ttgtagccct cgagtaccga
      541 ctgcttgcga ctcacgtcga ggctcgcaat catgctgtac atcggactcg tcgatgtatg
      601 catgttgaag ttctcgcgga agatgtgctc gttgaaggtc gggtcgttca cgtggatgta
      661 cgcggcctgc gatagggccg acagcgtctt gtgcgctgac tgcgtggcgt agtcggcgcc
      721 ggcctcaagc gcggtcggcc gcaatgccgg gtgaaagcgc gcatgcgcgt accaggcctc
      781 gtcgatcagg accttgatgt tgtgccggtg cgcggcgtcg atgatcggct tgaggttgta
      841 gcgcaggccg tcataggtgc aggacgtcag caccagcaat tcggcgtccg ggtgatcctc
      901 gatcgccttc atgatggtct tcgtgggcac cggaccgaat acgccgtact tgcggttcac
      961 cgacgaatcg agatacaccg ggcgcgcgcc cgacaggacg acgccgtgat ggaccgactt
     1021 gtggcagttg cggtcgagca gcagcttttc gcccggggca atcagcgttt gcaggatcac
     1081 cttgttggcg gtcgaggtgc cattggtggc gaaaaaagtg cgtcgcgcac cgaatgcctt
     1141 ggcggccatc tgctgcgctt cggcaatgac gccagtcggc tccatcagtg agtcgagcgc
     1201 cttgaccgag accgacaggt cggcgtcgaa cacgtgctcg cccatgaagt cgtagaactc
     1261 gttgacccag ggactgccgc gcatcgagtc accgctcgag tgcccgggcg tatgccaggc
     1321 gtccttggcc gccagcacat acgccttcag cgcatcgtaa aagggcgtcc gcgcacgttc
     1381 ggcgatctgc gcattcagga tgcggtacat cccgcgataa tcgcgctctt cgcggtaaaa
     1441 gtagccgtcg accgcttcgg cgaagagcgc gtccacgacc tggtcttcct tttccttctc
     1501 gatcaggatg tagatgtcga gctcggggcg cagggacgtg atgcgctgga ccagcttgag
     1561 cgccgacacc tgcaggttac gtgcggactc cttgtgacca tcgcggcgct tgcgcgtgtc
     1621 ctgcaccgca tacagcttgt cgtcgacgat ggcgatctgg atgtcacctt cgcgttcgat
     1681 caacgccaaa ccttccctgg cggtcaacgc gccgacgaaa gtgatgccga acgggttgtc
     1741 gagcgacctt gcggcggcat tcaggccctt gagcagttca cgcaggatca acggctcatc
     1801 gttgatgacg agcactttgg acaccggttt ggacatgcgc gtgggcggcg gggcgaaagg
     1861 gcaggggaag ggcggcttta tgcggcaatt gatgggcaaa tgcaagtaac ccagccgctt
     1921 tgaggcggcg caccgtgacc gagacctggc atgttgaaga gcgcatcgct tcgttgcgcc
     1981 gtcgggacgt ttccggctgc aacaccgtgc tcggataaaa tcgcgctcgc gttccgtcgg
     2041 cggtgcaact gcctgccgcg gcacggagca tgccgtgtca gaacctcccg cccccgccgc
     2101 gtccaaacct cgcgagttct tcatccacgg tgtgacgtcg accgggaagc ccttccggcc
     2161 gagcgactgg gcggaacgcc tgtgcggggt gatgtcgtgc taccggccgg gcggcattgc
     2221 ctcgggccgc gatgcctaca tcggttattc gccgtatgtg cggcccacgc tgatcggtgg
     2281 agtgaagtgc gtggtggtcg acgaacgcct gcgcgaactt gagcgcatgg cgttcgactt
     2341 cgtcatgagc tttgctcacg acaacgacct gccggtgtac gaagggtgtt cgatgcccga
     2401 cgcgaactga tcggcatcga gcttgggcgg actctggttg cccggtcctg aaggaccggg
     2461 gcctacgctg aagtgcactg ccggccttag gcgcccgaac gggcgcaccg gccgaccggg
     2521 accatcagtc catcgacttg atcgcagctg ccaggcggct cttgttgcga gctgccgtgt
     2581 tcttgtgaat gacgttcttg tcagcgatgg aatcgatcac gccggtggct gtgccgagca
     2641 cgtcaagcgc ggctttcttg tcaccggccg cgatcgcctt gcggaccgac ttgactgcgg
     2701 tgcggacacg tgagcgctgc gatgcgttct gggcattgcg tgcggtcgac tgacgtgcgc
     2761 gcttgcgggc ttgcttgctg tttgccatct gagttcctgt atttatccgt gaggctatcc
     2821 gtcaggcggg attcgtcccg tggttcccgt ccggtccttc gtatttgctc gtatctgtct
     2881 tggcgccgcc actgatcctg cacacggaac gtttcagcga gactttgacg ccggggcagt
     2941 cgcggaaagc cctaaactat atcaaatcaa ccgtttacca acaattcgcc accggttacc
     3001 aatcggaagc cggcagacaa ggcctgccgc cgcccccgcc agccggcaca atcccgcgca
     3061 gtgaatctct ttcgtgccct tgcaacggtc agcagcttca cgctgctatc acgcgtcacc
     3121 ggcctgatcc gcgacatgct gatctcgcgc ctgttcggcg cgagcgcgga aaccgacgcg
     3181 ttcaacgtcg cctttcgctt gcctaacctg ctgcggcgcc tgtttggcga aggagccttc
     3241 cagcaggcgt tcgtgccgat gctggccgat gtccgctccc gcggcgacga tccacagacc
     3301 acggcatttg ccgggcatgt agccagtgcg ctcttctggg tcctgctgat ggtgtcgatc
     3361 ctgggcgtcg tcggagcgcc gattctggtc tggctgattg cgtcgggtct ggcgcgcgac
     3421 ccggcgacgt tcgatctggc gaccgtcatg acgcgctgga tgtttccgta catcctgttc
     3481 atgtcgctgg tagcgctggc cgccggcgtg ctgaacacct acaagcgctt tgccgtgccg
     3541 gcgttcactc cggtcctcct caatcttgcg ttcatcggct gtgccctttg gcttgcgccg
     3601 cgcctgtcgc aaccgatcat ggcgctcgcg tttgccgtgc tgatcggagg cgcgctgcag
     3661 cttgggctgc agtgggccgc gctgcggcgt gccggtctgc gcctgcgcat cgggatgccg
     3721 cgcaccgcat tcgcggactc tgacgtgcgg cgtgtcgccg gtttgatgct gcccgccatt
     3781 ttcgcggtgt ccgttgcgca gctttcgatc atcatcaaca ccaacattgc atcgcacctg
     3841 accaccggga gcgtgacctg gctagcattc gccgaccggc tgatggagtt cccgacggcg
     3901 ctggtcgggg tcgccctcgg cacgatcctg ttgccgagcc tctcgaccgc atttgcggag
     3961 aagcgcgccg acgactattc gcggcttctc gactggggcc tgcggctgat ctttctgctg
     4021 gccctgccgg cggcgctggg cctcggcctg cttgctgaag gaatcgtgtc ggtgctgttc
     4081 caggggcgcg agttcaccgc gcacgacgta caccagacct cgatcgccgt catcggctat
     4141 gcggtcggcc tgatcgggct gatcgcggtc aagatcctgg ccccgggctt ctacgcgcag
     4201 aaagacatcc gcacgccggt gaagattgcg gtcacggtcc tgatcgccac gcagatcgcc
     4261 aacctcgtgc tcgtcccact gttcgcccat gccgggctgg cactgtcagt cagtctgggc
     4321 gcgtgcgcga atgcggtggc gctttacgtc ggtctcaaac gccgcggtat gtataccgcg
     4381 cggccgggct ggggcgggtt cgtactgcgc ctggccatcg cgctgctggt actgggcggt
     4441 gggttatggt gggcgcaacg cccgatcgac tggacggctc tgcagacttc accctggctg
     4501 cgggcgggtc tgctgggcac gatgatcacc gtcgcggccg gactttactt cgcggtgctg
     4561 ctcgccctcg gattccgccc gcgcgatttt cgacccgagc agggtcgagg agccgcacct
     4621 tcatgaccgt acctgcggtc gctgtgaacc agctcgatgc gcagctcgcc aacggcgttg
     4681 tcgcctgcct gacatgaaaa cgccgcctgg cggcggcgtt gctcaaggcg gatgctgagg
     4741 tcagccgacc tgcttcagca tcgtgccggc atcgctgacc tcgaacttgc cgggcgcttc
     4801 gatgttgagc gttttgacga cgccattttc cacgagcatc gaaaagcgct ggcagcgcac
     4861 gcccatgccg cgcgcggtca ggtcgagttc gagccctagc ttcttcgtgt agtccgccga
     4921 accgtcggcc atcatgcgca ccttgtcggt ggcgtgctgg tcgcgacccc aggcgcccat
     4981 cacgaacgca tcgttgactg aaatgcacca gatctcgtcc acgcccttgg acttcagctg
     5041 gtcgaccatc gcgatgtagc ccgggacgtg cttggcggaa caggtgggcg tatacgcacc
     5101 gggcaggccg aacacggcga tcttcttgcc cttgacgagg tcgcctacct tgaaggtgtt
     5161 cgggcccaat gaacagcccg cggtctcgac ttcgatgaat tcctgcagtt cgccgtccgg
     5221 cagcttgtct ccgaccttga tcgtcatggc gcgtcgtccc gtaatttgtt ggaggggagg
     5281 atcagtctag caacgtgcag cgatccgagg gctcagtcgc cgagtgcaac cccttcgcgg
     5341 cgagggtcag ctccgccggc ccagcccaag cgcccgtcgg tcaggcgcac gcgcacgatg
     5401 ccctgcaggc cgcttgccat gtcgatctcg cgcacgtcgt gtccgcgcgc cttcaggccg
     5461 ttgatcacgg cggcgccaat gcgccccttc tcgacttcgg tcggaccgtt gcgggagccg
     5521 aagttgggca gcgtgatggc ctgctggata tcgaggttcc agtcgagcac gcccaccagc
     5581 gtcttggcga catagccgat gatctgcgaa ccaccgggcg aacccagcgt ggccaccagc
     5641 gttccggtat cgcgctcgaa aaccagggtc ggtgccatcg agctgcgtgg gcgcttgccc
     5701 ggctgaacgc gattcgcaac cagctttcca tcgacctgcg gcaagaacga gaagtcggtg
     5761 agctggttgt tcagcaggaa ccctcgcacc atcacgcgcg cgccgaagac gtcctcgatc
     5821 gtcgtcgtca tcgacaccgc attgccgtgg tcgtcgacga ctgacacgtg cgacgtggcg
     5881 gccttcagcg gcgagggatc gggcgcccag gcgacggaag caccgggcgg gacgcccggt
     5941 tgagccgtgc ccaagctgcg tggaccgatc agttgagcgc gctgcttgag gtacgcggga
     6001 tcgagcaggc cggcgacgtt gaccgacacg aagtcactgt ccgcgacata cgacgcccgg
     6061 tcggcgaagg ccagtcgacc cgcttctgag aacaggtgca ccgcctcgac gcgcggctcg
     6121 agcgcgggcg cgtcgtccgg tgggaacacg gtgatgttcc gtgcagtgaa gatgccgagc
     6181 atctgcgcta ccgcgatgcc gcccgacgac ggcggcggca tgccgcacac gcgccagcgc
     6241 ttgtagtcgg tgcagaccgg ttcgcgctgt ctgacgcggt acgccgcgag atcggatgcc
     6301 gcgaggagcc cggcattctt ctgcgaccgc accgtcgcaa cgatgtcggc cgcaatcgga
     6361 cctgagtaga acgcgtcggc accacccgcc gcgatcgcgc gcagcgtcgc ggccagctgg
     6421 ggattgcgca ggaccgtgcc gaccgccttg gccgatccgt cggcgttgta gaagtacgct
     6481 gcggccgccg cgtcactctt caggtacttc tcgccggcca gcaactggtt caggcgcggc
     6541 gacatcggaa agccgttctc tgacagccgt atcgccggct cgaagagatc ggcccacggt
     6601 aactttccgt actgtcggtg cgccgtttcg agcaggcgca ccgcaccggg ggtgcccacc
     6661 gagcgtccgc cgacgacggc gtcgtagaac gccatcggct tgccgtcggc ctcgaggaag
     6721 agtcgctcgt ccacggccat cggtgcggtt tcacggccgt cgaaagcgac cacccgcttg
     6781 ccgtcccagt gcagcaggaa ggcgccaccg cccagaccgg aactctgggg ttcgaccaat
     6841 gccagcacca gctgaatcgt gatcgcggcg tcgaccgcgc tgccaccggc gcgcaggatg
     6901 tcgagaccgg cgagggtggc gagcggattg gccgaggcgg ccatgtgacg ctgggccgtc
     6961 gccgtttcca gtccggcgcg gaacgccgac gagggctcgg gctgcgacgg gcgctgcggc
     7021 gcggtggcgc aggccgcgag caatgcgacg gcgacgcagg cgatgggtcg gagcaagcgc
     7081 accatgatcg cctctttgcg aaaagcctcg ctttacttcg gctgcatgcg gatcgcgccg
     7141 tcgaggcgga tcgtctcacc gttcagcatg acgttttcga cgatgtgccg caccagcgct
     7201 gcatactcgt ccggtttgcc caggcgcgag ggaaagggca cctgcctgcc gagcgaatcc
     7261 tggatgtcct gcggcatgcc aagcaacatc ggcgtggcga agagccccgg tgcaatcgtc
     7321 acgcagcgga tgccgctgcg tgacaggtcg cgcgcgatcg gcagcgtcat gccgacgacg
     7381 ccgcccttgg aggcggaata cgcggcttga ccgatctggc cgtcgaaggc cgcgaccgag
     7441 gctgtgttga cgagcacgcc gcgttcgccc tcggccgtcg gctcctgctt tgacatggca
     7501 tcggcagcga gccggatcat gttgaaggtt ccga
//
LOCUS       DATIRT010000082         1930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1484623, whole genome shotgun sequence.
ACCESSION   DATIRT010000082 DATIRT010000000
VERSION     DATIRT010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1484623"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VLN25_02125"
     CDS             <1..402
                     /locus_tag="VLN25_02125"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HSQ09402.1"
                     /translation="LLLLFFLLVLFLTDVLQIVFSAALNGRALKSNPNKTVSGVLVGS
                     AGGALIGTLLWWMTPFTWWQALVMSLVIVGTGYLGTVVLASVKQSLGARQWDTEVVLT
                     RGVLERLDAMTFAAPLFWQITRYFWVIRADI"
     gene            682..1758
                     /gene="aroG"
                     /locus_tag="VLN25_02130"
     CDS             682..1758
                     /gene="aroG"
                     /locus_tag="VLN25_02130"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HSQ09403.1"
                     /translation="MRYMTDDLRIREIKELSPPAHLIREFPCEDVASVSVHESRQAIH
                     RILHGMDDRLVVVIGPCSIHDPSAALDYARKLKAERDRHAAHLEIVMRVYFEKPRTTV
                     GWKGLINDPDMDGSFRINHGLRVARELLMEINAAGLPAGCEYLDMITPQYIADFVAWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGNIRIASDAIKAAQQPHHFLSVTKGGHS
                     AIVSTKGNEDCHIILRGGRHPNFDAPSVEAACSELGKAGLAQRLMIDASHANSQKNHA
                     NQSSVAGNVGDQLAAGDTRIIGVMVESNIVAGRQDLIPGKPLTYGQSVTDACLGWEDS
                     VKVLETLAESVRKRRLQTANASAK"
BASE COUNT          371 a          605 c          610 g          344 t
ORIGIN      
        1 ctgctgctgc tgttcttcct gctggtgctg ttcctgaccg acgtgttgca gatcgtgttc
       61 agtgcggccc tcaatggacg tgccttgaag tcgaacccca acaagaccgt cagcggcgtg
      121 ctggtaggca gtgcgggcgg agcactgatc ggcacgctgc tctggtggat gacacccttc
      181 acctggtggc aggcgctggt catgtcgctt gtgatcgtcg gaaccggcta tctgggaacg
      241 gttgtcctgg catcggtcaa gcaaagtctc ggagcgcgcc agtgggatac cgaggtggtg
      301 ctgacacggg gcgtgctcga gcggctggac gcgatgacgt tcgcggcgcc gctgttctgg
      361 caaattaccc ggtatttctg ggtcatccgc gccgatatct gaccaacgtc aacggcggtg
      421 ggcttgccgc acgatgcaat cctccggtat cttgtgggtt cgcaccgcgc ttaggcggtg
      481 cgtgctgacg atgaatccga ccgcgcgctt ttacttcttc tacttttatg gctttccccg
      541 cctggcggag agagtcgaac ggcgcgcgta acgaacagac cgaatcgacc cgaaccgcca
      601 ggccacaaac ctggcggttt tttttcgcca ggtgggccgg atgaaagcaa tcgaggaccc
      661 acgatggacg gagcacgaac catgcgctac atgaccgatg acctgcggat ccgcgagatc
      721 aaggaactct cgcctccggc acacctgatc cgcgagtttc catgcgaaga cgtggcgtcg
      781 gtctccgtac acgagagccg gcaggcgatt caccgcatcc tgcacggcat ggacgatcgg
      841 ctcgtcgtcg tcatcggtcc gtgctcgatc cacgatccgt cggcggcgct ggactacgcg
      901 cgcaagctca aggccgagcg cgaccgccat gcggcccatc tcgagatcgt gatgcgcgtt
      961 tacttcgaaa agccgcgcac gaccgtcggc tggaaggggc tcatcaacga cccggacatg
     1021 gatggcagtt tccgaatcaa ccacggctta cgcgtcgcgc gcgagctgct catggagatc
     1081 aacgcagccg gccttcccgc cggctgcgag tatctcgaca tgatcacgcc gcagtacatc
     1141 gctgactttg tcgcatgggg cgcgatcggc gcacgcacga ccgagagcca ggtgcatcgg
     1201 gaacttgcct cgggcctgtc gtgcccggtc ggcttcaaga atggcaccga cggcaacatc
     1261 cggatcgcgt ccgacgcgat caaggcggct caacagccgc atcacttcct gtcggtcacg
     1321 aagggagggc attccgccat cgtctcgacc aaaggcaatg aggactgcca catcatcctg
     1381 cgcggtggca ggcacccgaa tttcgacgcg cccagcgtcg aggcggcgtg ctccgaactc
     1441 ggcaaggccg gtctcgcgca acgcctgatg atcgacgcga gccatgccaa cagccagaaa
     1501 aaccacgcca accagagctc cgttgccggg aacgttggcg accagctcgc cgcaggtgac
     1561 acgcggatca ttggcgtgat ggtggagagc aacatcgtcg ctggccggca ggacctgatc
     1621 ccaggcaagc cgctgaccta tggccagtca gtcacggacg cgtgcctggg atgggaggac
     1681 agcgtaaagg ttctcgagac actggccgag tcggttcgca agcgacggct tcaaacggcc
     1741 aatgcctcgg caaagtgaag cagcccgcag cgttgccgct tgggtgaagg actacttcac
     1801 gagatcgcgg tacgcgtgcc agctcgcgtg gccaagcagc gggaagatga tcaccagtcc
     1861 cagcatcagc gtcgcgaagc cgatggcggt cagcactgcg atcatcgcgg cccagaccag
     1921 cgttgcgccc
//
LOCUS       DATIRT010000083         2106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_206652, whole genome shotgun sequence.
ACCESSION   DATIRT010000083 DATIRT010000000
VERSION     DATIRT010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206652"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2106
                     /locus_tag="VLN25_02135"
     CDS             <1..>2106
                     /locus_tag="VLN25_02135"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081256.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. Subunit beta'
                     binds to sigma factor allowing it to bind to the -10
                     region of the promoter; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HSQ09404.1"
                     /translation="VDSLLDNGRRGKAMTGANKRPLKSLADMIKGKGGRFRQNLLGKR
                     VDYSGRSVIVVGPQLKLHQCGLPKLMALELFKPFIFNKLELMGLATTIKAAKKMVESQ
                     EPVVWDILEEVIRQHPVMLNRAPTLHRLGIQAFEPVLIEGKAIQLHPLVCAAFNADFD
                     GDQMAVHVPLSLEAQLEARSLMLASNNVLFPSNGDPSIVPSQDIVLGLYFATREKING
                     MGEGSFFADLAEVHRAYENRKVELQTRITVRIREVEVNPETGEKTPKITRYETTVGRA
                     FMSEILPAGLPFSVLNKPLKKKEISKLINTAFRKCGLRETVIFADKLMQSGFTLATRA
                     GLSIAIDDMVVPAQKGEIIRSAEKEVKEIEAQYTSGLVTQGERYNKVVDIWGRAGDHV
                     GKVMMEHLATEQTIDRHGQEVKQESFNSIWMMADSGARGSVAQIRQLAGMRGLMAKPD
                     GSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTRRLVDVTQDLVV
                     TEDDCGTINGVAMRALVEGGEVIEPLRDRILGRVAAVDVINPDTQDVLYPQAGLLDED
                     AVEQIEALGIDEVRVRTPLTCETRYGLCAKCYGRDLGRGVLVNSGEAVGVIAAQSIGE
                     PGTQLTMRTFHIGGAASRAAVASGVEAKSNGTVRFTATMRYVTNVKGEQVAISRSGEV
                     TIVDDNGRERERHKVPYGAM"
BASE COUNT          421 a          637 c          704 g          344 t
ORIGIN      
        1 gtcgactcgc tgctcgacaa cggccgccgc ggcaaggcga tgacgggcgc caacaagcgt
       61 ccgctgaagt cgctggccga catgatcaag ggcaagggtg gccgcttccg ccagaacctg
      121 ctcggcaagc gcgtcgacta ctcgggccgc tcggtcatcg tcgttggtcc gcagctcaag
      181 ctgcaccagt gcggcctgcc gaagctgatg gcgctggagc ttttcaagcc gttcatcttc
      241 aacaagctcg aactgatggg gctggcgacc acgatcaagg cggccaagaa gatggtcgag
      301 tcgcaggagc cggtggtgtg ggacatcctc gaagaggtga tacgccagca cccggtgatg
      361 ctgaaccgcg caccgacgct gcaccgcctc ggcatccagg cgtttgagcc ggtgctgatc
      421 gagggcaagg caatccagtt gcatccgctc gtttgcgcgg cgttcaacgc cgacttcgac
      481 ggcgaccaga tggccgtgca cgtgccgctg tcgctcgagg cgcagctcga agcgcgttcg
      541 ctgatgctgg cgtcgaacaa cgtgcttttc ccgagcaacg gcgatccgtc gatcgtgccg
      601 agccaggaca tcgtgctggg cctgtacttc gccacccgcg agaagatcaa cggaatgggc
      661 gagggctcgt tctttgccga tctggccgag gtgcatcgcg cctatgagaa ccgcaaggtc
      721 gagctgcaga cgcgcatcac ggttcgcatc cgcgaggtcg aagtcaatcc ggaaaccggg
      781 gagaagacgc cgaagatcac gcgctacgag accacggtcg gacgcgcttt catgtccgag
      841 atcctgccgg ccggcttgcc gttttcagtg ctgaacaagc cgctgaagaa gaaggaaatc
      901 agcaaactca tcaacacggc gttccgcaag tgcggactgc gcgagacggt gatcttcgct
      961 gacaagctga tgcagtcggg attcacgctg gccactcgcg cgggtctttc gatcgccatc
     1021 gacgacatgg tcgtgccggc gcagaaaggg gaaatcatcc gcagcgccga gaaggaagtg
     1081 aaggagatcg aggcgcagta cacctcgggt ctggtcactc agggcgagcg ctacaacaag
     1141 gtggtcgaca tctggggccg cgccggcgat cacgtcggca aggtcatgat ggaacacctc
     1201 gcgaccgaac agacgatcga ccgccacggt caggaagtga agcaggagtc gttcaactcc
     1261 atctggatga tggccgactc aggcgcgcga ggttcggtgg cacagattcg ccagctcgca
     1321 ggcatgcgcg gcctgatggc caagccggac ggctcgatca tcgaaacgcc gatcacggcg
     1381 aacttccgcg agggactgaa tgtcctgcag tacttcatct cgacgcacgg cgcacgcaag
     1441 ggcctggccg acacggcgct taagactgcg aactcgggct acctgacgcg gcgactggtc
     1501 gacgtcacgc aggacctggt cgtgaccgag gacgattgcg gcacgatcaa tggcgtcgcc
     1561 atgcgtgcac tggtcgaagg tggcgaggtc atcgagccgc tgcgtgaccg tatcctcggc
     1621 cgcgttgcag ccgtcgatgt gatcaacccg gacacgcagg acgtgctcta tccgcaggcc
     1681 ggtctgctcg acgaagacgc ggtcgagcag atcgaggcgc tgggcatcga cgaagtgcgc
     1741 gttcgtacgc cactcacgtg cgaaacccgt tacggcctgt gcgcgaagtg ctacggccgc
     1801 gatctcggca ggggcgtgct ggtcaactcg ggggaagccg tcggcgtcat tgccgcacag
     1861 tcgatcggcg agccgggcac gcagctgacg atgcgaacct ttcacatcgg tggagcggca
     1921 tcgcgcgcag cagtggcgtc gggtgtcgag gccaagagca acgggacggt gcgctttacc
     1981 gcgacgatgc gctatgtcac gaacgtcaag ggtgagcagg tcgccatctc gcgatcgggc
     2041 gaagtcacca tcgttgacga caatggccgg gagcgtgagc gccacaaggt gccctatggc
     2101 gccatg
//
LOCUS       DATIRT010000084         3959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_993501, whole genome shotgun sequence.
ACCESSION   DATIRT010000084 DATIRT010000000
VERSION     DATIRT010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993501"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..86)
                     /locus_tag="VLN25_02140"
     CDS             complement(<1..86)
                     /locus_tag="VLN25_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HSQ09405.1"
                     /translation="MAVADINKSEIVAKYQRAQADTGSPEVQV"
     gene            complement(583..2133)
                     /locus_tag="VLN25_02145"
     CDS             complement(583..2133)
                     /locus_tag="VLN25_02145"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010114575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HSQ09406.1"
                     /translation="MTDRLFIFDTTLRDGEQSPGASMNKEEKLRIAMQLERLRVDVIE
                     AGFAASSNGDFEAVRAIANAVKDSTVCSLARANDRDISRAAEALKGARSSRIHTFIAT
                     SVLHMEKKLRMTPDQVFEQARLAVRFARQFTDNVEFSPEDGYRSEPDYLCRVLEAVIA
                     EGATTINIPDTVGYAIPELYGLFLRDLRERIPNSDKAVWSVHCHNDLGMAVANSLAGV
                     KIGGARQVECTVNGLGERAGNCSLEEVVMAVRTRRDFFGLDVGVDTTQIVPASKLVSG
                     ITGFPVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDVGWHANKIILGKLSG
                     RNAFKQRVHELAIPIESETELNAAFARFKELADRKSEIYDEDIQALFSDETVTPHDEH
                     FKLLSLSQRSETGERPHARVVISIGNAEVSAESDGNGPVDASLKAIESQVRSGCELLL
                     YSVNAITTGTESQGEVTVRLARAGRIVNGTGADPDIIAASAKAYLNALNKLHSGVERL
                     NPQTSLAP"
     gene            complement(2391..3137)
                     /gene="pssA"
                     /locus_tag="VLN25_02150"
                     /pseudo
     CDS             complement(2391..3137)
                     /gene="pssA"
                     /locus_tag="VLN25_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420002.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
     gene            complement(3224..3862)
                     /locus_tag="VLN25_02155"
     CDS             complement(3224..3862)
                     /locus_tag="VLN25_02155"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HSQ09407.1"
                     /translation="MNYPHPLLAREGWPFIAGAVLLALATWWFVGFAWSLPLWLVAVF
                     VIQFFRDPPREVPGLPSAVLSPADGRIVKVQKAHDQITDRETLLISVFMNVFNVHSNR
                     SPVDGSVETIKYSPGSFVNADLDKASTENERNAMVMRLESGERITVVQVAGLIARRIL
                     CYVNAGDRLTRGQRYGFIRFGSRVDVYMPPSARPRVAVGDIVYATTTVLAEL"
BASE COUNT          759 a         1269 c         1265 g          666 t
ORIGIN      
        1 acctgcactt caggcgatcc ggtgtcggcc tgcgcgcgct gatacttcgc gacgatttcc
       61 gacttgttga tatctgcaac tgccatggtt tccctttcag atcaatcagc tagacacgcg
      121 gacacggaag gatggccgtg ccgtgaagcg cgggattata tctcatcgcc gctcgcgtac
      181 gcgaccctgc gggtcgcacg tgcgctcgcg gcggtcacgg cttcgccgct caacaatatt
      241 gatcagtcgg ctgcgctccg gagcggccgc gcgctccgcc gaagcaacag agcaaagctc
      301 ccacccggcc tccctccagg agggaggagc aagttaggtc atcgccttgc cggtctccat
      361 cgcgaggagc cgggacaagt tcgaggctgc cggcaggccg cggtcatccc tcccactcgg
      421 cgggacggcc agggtgggtg ccaatgtctt tgttactcgg cggagcgcgc ggccgctccg
      481 gagcgcagcc gaccgatcag tcttgagcgg ccgagcgaag cgacggccgt gccgctccga
      541 gtgcacgtgc gatggcaaag ccatcgcgta cacgaggagc gcctatggcg cgagagaggt
      601 ttgcggattg agtcgctcaa ccccgctatg cagcttgttc agggcgttca gatacgcctt
      661 ggccgaggcg gcgatgatgt cggggtcggc gccggtaccg ttgacgatgc ggccggcgcg
      721 cgccagccgc acggtcacct cgccctgcga ctcggtgccg gtcgtgatcg cattgaccga
      781 gtaaagcagc agttcgcatc cgctcctgac ctggctttcg atcgctttga gcgaggcatc
      841 gaccgggcca ttgccatccg actcggccga gacttcggca ttgccgatcg agatgacgac
      901 gcgcgcgtga gggcgctccc cggtttctga gcgctgcgac agcgacagca gcttgaagtg
      961 ctcgtcgtgc ggcgtcaccg tttcatctga gaacagggcc tggatgtcct cgtcgtagat
     1021 ctccgacttg cgatcggcga gttccttgaa gcgggcaaaa gcagcgttca gctcggtctc
     1081 gctctcgatc gggatcgcca gttcatggac gcgctgcttg aacgcattgc ggccggagag
     1141 cttcccgaga atgatcttgt tggcgtgcca cccaacatcc tcggcgcgca tgatctcgta
     1201 ggtgtcgcgt gccttgagca cgccatcctg gtgaattccg gaggcatgcg cgaacgcgtt
     1261 ggccccgacc accgccttgt tgggctgcac cgggaagccg gtgatgccgg acaccagctt
     1321 cgacgccggc acgatctgcg tggtgtcgac gccgacatcc agtccgaaga agtcacggcg
     1381 cgtccgcacc gccatcacga cttcctcgag cgagcagttg ccggctcgct cacccagccc
     1441 gttcacggtg cactcgacct ggcgcgcgcc accgatcttt acgccggcga gcgaattggc
     1501 caccgccatg cccaggtcat tgtggcaatg cacgctccag acggccttgt cggagttcgg
     1561 aatgcgctcg cgcaagtcgc gcaggaacaa gccgtagagc tcggggatcg cataaccgac
     1621 ggtatccggg atattgatcg tcgttgcccc ttcggcaatg acggcctcga gtacccggca
     1681 caggtaatcc ggctcggagc gatagccgtc ttccggcgag aactcgacgt tgtcggtgaa
     1741 ctgtcgggcg aaccgcaccg caagcctggc ctgctcgaag acctggtccg gtgtcatgcg
     1801 cagcttcttt tccatgtgca gcaccgacgt tgcgatgaac gtgtggatgc ggctggaccg
     1861 cgctcccttg agcgcctcgg cagcccggct gatgtcgcga tcattggcgc gtgccagcga
     1921 acagaccgtc gagtccttga ctgcattggc gatcgcgcgc accgcctcga aatcaccgtt
     1981 ggacgaggcg gcgaatcctg cttcgatgac atcgacgcgc agccgctcca gctgcatcgc
     2041 gatgcgcagc ttttcttcct tgttcatgga ggcgccaggg ctctgctcgc cgtcgcgcaa
     2101 agtggtgtcg aagatgaaga gtcggtcggt catgagtcgc tccagcgcga aatggtcttt
     2161 gctgccaggc tcttggcgct ggcagcggca ctacacgagt tgtgggtcgg gggtcagctg
     2221 cgcgcggggc gcagcagcag cagaaacaac ggcagccggc gcgagaggtc ggcgatgggg
     2281 cgaggcgcgg tgaacgtggc gcggcacatg agcggcacta tatgacgatt ccgcggatgc
     2341 aacaacctag actgcatggc cggccgcgca aacgtgggtg accatgcggc ctagcccgaa
     2401 cgatcggcat cgcgcgcgac cggcttggcc ggattcggct cgcctcggaa ccactgccag
     2461 ccccaataga catacccgga cagcgcgtag atacagaaca gcgtgaacag tgcgatcggc
     2521 ggatccgaac tgaccacgaa gatcacgacg atcagcgcca ccatcaccca gaacggaacg
     2581 ctgcggccaa cgtggaaact cttgccgctg tagaacggcg cactcgagac catggtcagt
     2641 cccgtataga cggtcaggcc gaacgcaacc cacggcatga cgctgtcgcg caccggaatc
     2701 ttgttgtcga tggccagcca gacaaagccg gcgatcagtg ccgccgccgc cgggctcggc
     2761 agcccctgga agaagcgctt atcgacaacg ccgatgttcg aattgaagcg cgccagtcgc
     2821 agcgcagccc cagcacagta aacgaacgcg cctgcccagc cccagcggcc gaggtcgctc
     2881 agcacccatt cgtacatcac caatgcgggc gcgacgccga acgaggtcat gtcggacagc
     2941 gaatcgaatt gctcgccaaa ggcgctttgc gtattggtga gccgcgcgac ccgtccatcc
     3001 atgccgtcga ggaccatggc cgcaaagatg gcgatcgcag acagttcgaa acgctggttc
     3061 attgcctgca caatggcaaa gaacccggca aacagcgccg ccatcgtgaa cagattcggc
     3121 agcaggtaga tgccgcgtcg cctgctgcgc gtcgggctgc tgtccagcgg aaccggggct
     3181 cccgtaagcg ggaaagcgcg tgacttgcgg cggcgaaagc gcatcagagc tcggcgagca
     3241 cggtagtggt ggcgtaaaca atgtcaccga cggcgacccg cggccgtgcc gagggcggca
     3301 tatagacatc aacgcgggaa ccgaagcgga tgaacccgta gcgctggccc cgcgtcagcc
     3361 ggtcgccggc gttcacgtag cagaggatgc ggcgcgcaat cagcccggcg acctggacaa
     3421 cggtgatgcg ctccccagac tcgaggcgca tcaccatggc attgcgttcg ttttccgtgg
     3481 acgccttgtc aaggtcagca ttgacgaagc tgccgggcga atacttgatc gtctcgaccg
     3541 acccgtcgac cggtgagcga ttgctgtgta cgttgaaaac gttcatgaac acgctgatca
     3601 gcagcgtctc ccgatccgtg atctggtcgt gcgccttctg cactttgaca atgcggccat
     3661 cggccggcga gagcaccgcc gacggcaggc cgggcacctc gcgcggcgga tcgcggaaga
     3721 actggatcac gaagacagcc accagccaga gcggcagcga ccaggcaaaa cccacgaacc
     3781 accacgtggc aagcgccagc agcacggcgc ccgcgatgaa aggccagcct tcccgcgcaa
     3841 gcaggggatg cggatagttc aacgaagtga cccagaagtg acccgcatag gagaaggccc
     3901 aatggtatcg gaacaggcct cgctcgatgc agtcgacttc cgtccttgtc ccggccgac
//
LOCUS       DATIRT010000085         3531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1091790, whole genome shotgun sequence.
ACCESSION   DATIRT010000085 DATIRT010000000
VERSION     DATIRT010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091790"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VLN25_02160"
     CDS             complement(<1..510)
                     /locus_tag="VLN25_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09408.1"
                     /translation="MNREALLNPTFTQRRFPMARALLGLMLGLAATSAWSQNAVVGRQ
                     LYLNTNGSPLSCGTGACHAADPALRVRGIEKGTTAAAILNAIATKNEMRFLASAPYTV
                     SAQQAADIAAYIVNPAAANGASITLSATTLAFGSTQIAVANTTTTPATITLTNSGGGP
                     LTLTAIGKTG"
     gene            complement(563..1450)
                     /locus_tag="VLN25_02165"
     CDS             complement(563..1450)
                     /locus_tag="VLN25_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSQ09409.1"
                     /translation="MFLRSRVSPLLRVVGVAMLIVGAAPCSAQTSGDSTAGQQLFTTT
                     LSPQCSGCHSTTTPAAANSLTSIRNAITNRATPAGAAGTMSFAKALEALNAALTGSTL
                     GGAVTGMNGFYTLTTTQRGDLAAYLAGLAGPAPIVSYTPTGGAIFTATAVGASSNATV
                     TIRNTGTAPLTFATNNAVTIATGGDASDFLIASATCNPGSTLAANSGSCTITATFTPA
                     AGASLTRTASIGIATTTSVSLVPMQGSVSVPAAAAPPTGSANAPSSGGGGALSWPAVI
                     LILTSLLCCGRRPLVGGRR"
     gene            complement(1622..2827)
                     /locus_tag="VLN25_02170"
     CDS             complement(1622..2827)
                     /locus_tag="VLN25_02170"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009977966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HSQ09410.1"
                     /translation="MADEKSLPDFDFDTRAVRAGTLRSPFNEHSEAVFLTSSFVFDNA
                     AQAAERFSGAEEGYFYSRFTNPTVRMFEQRLASLEGAEDCIATASGMAAITATCLAIL
                     KSGDHVVCGANVFGATLQLYSTVLGRFGIDVSYVDAADPAAWRAAVTPRTRLLYCETP
                     SNPLMDIVDLTAFAALGRETGALTVVDNCFCTPVLQRPLEFGVDAVIHSATKYLDGQG
                     RVLGGAILGSRKLIREQVLPVLRTTGPSLSPFNAWVLLKGLETLAVRMRAQSSAALEL
                     ATWLERQPQVARVHYPGLPTHRQAHLCSRQQASGGAVVAFEVDGETPAAARARAWAVI
                     DRIQLMSITANLGDTRTTITHPSTTTHGRISPEARERAGIREGLIRIAVGLESIADIQ
                     RDLAHALAD"
     gene            complement(2950..>3531)
                     /locus_tag="VLN25_02175"
     CDS             complement(2950..>3531)
                     /locus_tag="VLN25_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HSQ09411.1"
                     /translation="LAMKLGLEFREGFFKNRYVGRTFIMPGQSVRRKSVRQKLNALPT
                     EFKGKNVLLVDDSIVRGTTSREIVHMARESGANRVIFASAAPPVRFPNVYGIDMPTRS
                     ELIAHGRSDEEIQRMIEADGLVYQDIDGMKRSVSELGPHLTSFEASCFDGHYITGDVT
                     PEYLDRLEATREAAIAVEAEALSQLNLNLASAD"
BASE COUNT          639 a         1109 c         1152 g          631 t
ORIGIN      
        1 gccggtcttg ccgatggccg tgagggtcaa cggcccgcca ccgctgttgg tcagagtgat
       61 ggtcgcgggt gttgtcgtcg tgttggcaac ggcgatctgg gtcgaaccga aggcaagcgt
      121 tgtagccgac aacgtgatcg acgcgccatt agccgctgcg ggattgacga tgtacgccgc
      181 gatatcagcc gcctgctgcg ctgaaacagt gtagggagcg ctggcaagaa accgcatctc
      241 gtttttcgtg gcgatcgcgt tcaggatcgc ggccgcagtg gtgcccttct caatgcccct
      301 gaccctgagc gctggatccg cggcgtggca agcaccggtc ccgcaactca agggcgaccc
      361 gttcgtgttg aggtagagct gcctgcccac gacggcgttc tgagaccacg ccgaggtggc
      421 cgcgaggccc aacatcagtc cgagcaatgc gcgcgccatg ggaaaccttc tttgggtaaa
      481 tgtcggattc aaaagtgcct cgcgattcat acgcattagc tccactgtct ttagcggttt
      541 cctgcccgca ctctttaccg cctcaacgcc gaccgccgac gagcggtcgc cgcccacagc
      601 acaagagcga cgtcagtatc aggatgacgg caggccacga cagggcgccc cctccacccg
      661 aacttggcgc attggctgaa ccggtcggcg gcgccgctgc tgccggaacg gaaacggaac
      721 cctgcatcgg caccagcgag acactggtcg tcgttgcgat gccgatcgat gcggtacgcg
      781 tcagtgacgc accggcggcc ggagtgaatg tggcggtaat cgtgcaactg ccgctgttgg
      841 cggcgagcgt gcttccagga ttgcaggtgg ccgacgcgat gaggaagtcg gaggcgtcac
      901 cgcccgtcgc gatcgtcacg gcattgttcg tcgcgaacgt cagcggcgcc gtaccggtgt
      961 tccggatcgt gaccgtcgcg ttcgatgaag caccgaccgc ggtggcagtg aagattgcac
     1021 caccggtggg cgtatagctg acgatcggag ccggaccggc caggcctgcc aggtacgccg
     1081 cgagatcacc gcgctgagtc gtcgtcagcg tgtaaaagcc attcattccg gtcactgcgc
     1141 caccgagtgt gctgccggtt agcgccgcat tgagtgcctc gagtgccttg gcgaaactca
     1201 tggtgccggc cgcgccggcg ggtgttgcgc gattggtaat tgcgttgcga atgctcgtca
     1261 gcgagttggc ggccgccgga gttgtcgtcg aatgacaacc ggagcactgc ggcgaaagcg
     1321 ttgtcgtgaa caattgctgg ccggcagtcg agtctccgct cgtctgtgcc gagcatggcg
     1381 cggcaccgac gatcaacatc gctacgccga caacgcgcag caaagggctc acccgcgagc
     1441 gcagaaacaa cgtgttctcc caagaggttc gcgcgccaac ctcgcacgac tgcgagtgcg
     1501 cctcacgagt cgaattctct tcgagaggga gtggactttc gatccgcctt acgtcttagc
     1561 gccagcggtt cgggagccgg cgtccggccg gcagatcatc cggatgacct agcgtgtgag
     1621 ttcagtccgc cagcgcatgc gcgaggtcac gctggatatc ggcgatcgat tcgaggccga
     1681 ctgcgattcg gatcaggcct tcacgtatgc cggcgcgctc gcgcgcttcc gggctgatgc
     1741 gtccatgcgt cgtggtggac ggatgagtga tcgtcgtgcg ggtatcaccg agattcgccg
     1801 tgatcgacat cagctggata cggtcgatca ccgcccaggc acgcgctctc gcggcggccg
     1861 gcgtctcgcc gtcgacctcg aatgcgacca ccgcgccacc gctcgcctgc tggcgcgagc
     1921 acaggtgggc ctgccggtgg gtcggcaggc ccggatagtg gacacgcgcg acctgcggct
     1981 ggcgttccag ccacgtcgcc agttcaagcg cggccgaact ctgtgcgcgc atgcgcacag
     2041 ccaatgtttc caggcccttc aggagtaccc acgcgttgaa cggcgacagg ctgggcccgg
     2101 tcgtgcgcag cacgggaaga acctgttcac ggatgagttt tcgcgagccg aggatggcgc
     2161 cgccgagtac ccttccctgc ccgtcgagat acttcgtcgc cgaatgaatc acggcatcta
     2221 cgccgaactc gagcggcctc tgcagcaccg gcgtgcagaa acagttgtcg accacggtga
     2281 gcgcgcctgt ttcgcgtccc agggccgcga acgcggtgag atcgacgata tccatcagcg
     2341 gattcgaggg cgtctcgcaa taaagcagcc gcgtgcgggg tgtcaccgcc gcgcgccagg
     2401 ccgctggatc agcggcgtcg acgtagctca cgtcaatacc aaagcgcccg agcacggtgg
     2461 agtagagctg cagcgtcgca ccaaagacgt tggccccgca gacgacgtga tcgccggact
     2521 tcaggatcgc aagacacgtc gcggtgattg ccgccatgcc cgaggccgtg gcaatgcagt
     2581 cctcggcgcc ctcgagcgac gcgagtcgct gctcgaacat gcggacggtc gggttggtga
     2641 agcgcgagta gaagtagcct tcttcggcgc cagaaaagcg ctcggccgcc tgcgcagcgt
     2701 tgtcgaagac aaagctcgac gtcaggaaca ccgcctcgct gtgctcgttg aagggcgagc
     2761 gcagcgtgcc ggcacgcacc gcgcgcgtgt cgaagtcgaa atcgggaaga gacttttcgt
     2821 cggccataaa aaaacccgcc ggcagaagcg cagcgggttc attcgagccc tcgctttagc
     2881 cgtatttgtt aagcgcccgc aagctgagac ttcaaatcgg cgcgatcgga ggattgtata
     2941 cgagccggat cagtctgccg aggcaaggtt caggttcagc tgcgacagtg cctccgcctc
     3001 gacggcaatg gccgcctccc gggtcgcttc gaggcggtcg aggtactccg gcgtgacgtc
     3061 gcccgtgatg tagtggccgt cgaagcaact cgcctcgaag ctcgtcagat gcgggccgag
     3121 ctcggacact gaccgcttca tcccgtcgat gtcctggtag acgaggccat cggcttcaat
     3181 catgcgctgg atttcctcat ccgagcggcc gtgcgcgatg agttcgctgc gtgtcggcat
     3241 gtcgatgccg tacacgttgg ggaaacgcac cggcggcgcg gccgatgcga agatcacccg
     3301 gttggcaccg gactctcgcg ccatatggac gatctcacgc gaggtcgtgc cacggacgat
     3361 cgaatcatcg accagcagca cgttcttgcc cttgaactcg gtcggcagtg cattgagctt
     3421 ctgccgtacg ctcttccggc gcaccgactg tcccggcatg atgaaggtac gaccgacata
     3481 gcggttcttg aagaatccct cgcggaactc aagcccgagc ttcatcgcca g
//
LOCUS       DATIRT010000086         3072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_993658, whole genome shotgun sequence.
ACCESSION   DATIRT010000086 DATIRT010000000
VERSION     DATIRT010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993658"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="VLN25_02180"
     CDS             complement(<1..595)
                     /locus_tag="VLN25_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HSQ09412.1"
                     /translation="MATPIYLASRSPRRRELLKQISVKFDPLLLRLATPRGPDVDEAQ
                     HGGETPADYVERTAREKASFGLEVLSMRSMIFRPVLAADTVVIVDGEVLAKPANPEEA
                     AAFMRRLSGRTHEVRTAIALAIQGPLLNATSVSTVTFRKLDDEEIKRYCATSEPYDKA
                     GGYGIQGIAAVFVERIEGSYTGVMGLPLFETARLLQQA"
     gene            complement(613..1086)
                     /gene="rlmH"
                     /locus_tag="VLN25_02185"
     CDS             complement(613..1086)
                     /gene="rlmH"
                     /locus_tag="VLN25_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HSQ09413.1"
                     /translation="MRISIVAIGQKQPAWADEAVNDYLARFPADFSVELKELKAEART
                     GRADEAERSRTAEAKRLRAALPVGSTVVALDELGKDWSTQQLADQLRRWRDGAESVAF
                     VIGGPDGLDDAFKREARMQLKLSSLTLPHALARVVLAEQLYRAWSILARHPYHRV"
     gene            complement(1117..1518)
                     /gene="rsfS"
                     /locus_tag="VLN25_02190"
     CDS             complement(1117..1518)
                     /gene="rsfS"
                     /locus_tag="VLN25_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HSQ09414.1"
                     /translation="MDIRKLQRVIVDALEDVKGQDIKVFNTTHLSDMFDRIVIASGTS
                     NRQTRSLASSVAEKVKAAGGSVISMEGVDTGEWVLVDCGDAVVHVLQPAIRAYYNLEE
                     IWGDKSVRLKSSAPASIAGKKSRKAPARRTA"
     gene            complement(1508..2188)
                     /gene="nadD"
                     /locus_tag="VLN25_02195"
     CDS             complement(1508..2188)
                     /gene="nadD"
                     /locus_tag="VLN25_02195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HSQ09415.1"
                     /translation="MEPIGLLGGSFDPIHVGHLQLARDALANLPIAEIHLIPAAQPWQ
                     KEALTDAVHRARMIRLAIDNELPFTRRRMVLNMSEIERGGPTYTIDTLREQRGLVGRD
                     TPLVLIMGSDQFERFETWREWERIPDLVHLAIARRAGAIPSLGPTLQRLRDARYLKYP
                     GDLVDSPAGHIVDLAMTPVDASATEVRRLLAQPVTSADAARLAAMVSPPVLDYIHSHH
                     LYTGRHGH"
     gene            complement(2202..3062)
                     /gene="hemF"
                     /locus_tag="VLN25_02200"
     CDS             complement(2202..3062)
                     /gene="hemF"
                     /locus_tag="VLN25_02200"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HSQ09416.1"
                     /translation="MVGTFEKLDGRPFLADTWEKPTGAPLGGGGLTRIIEDGNLLERG
                     GVGFSHVIGSTLPPSATAQRPELAGRGFEAMVVSLVFHPRNPHVPTVHMNVRFFIAKK
                     TGEPDVYWFGGGMDLTPYYATEEDARHFHATCKQALAPFGADKHPRFKKWCDEYFFLK
                     NRNEPRGIGGVFFDDFHELGFDASFELMRAVGDAFLDAYVPIVERHRHRDFGPRERDF
                     QAYRRGRYVEFNLVFDRGTLFGLKSGGRTESILMSLPPLVHWKYDWHPEPGSAEARLY
                     TDFLRPREWA"
BASE COUNT          509 a          970 c         1038 g          555 t
ORIGIN      
        1 cggcctgttg cagcagccgc gcggtctcga acagcggcag gcccatcacg ccggtgtagc
       61 tgccttcgat gcgttcaaca aagaccgcgg caattccctg gatgccgtag ccgccggcct
      121 tgtcataggg ctccgaagtc gcgcaatagc gtttgatctc ttcgtcgtcg agtttgcgaa
      181 acgtcacggt ggacaccgaa gtggcgttca gcagcgggcc ctggatggcc agtgctatgg
      241 cagtgcggac ctcgtgcgtg cggccggaga gccggcgcat gaaggcggcg gcttcttcgg
      301 ggttggccgg cttggcgagc acttcgccat cgacgatcac gaccgtatcg gccgccagca
      361 ccggacggaa gatcattgag cgcatgctca ggacctcgag gccgaagctc gctttttcgc
      421 gtgcggtgcg ctcgacatag tcggccggcg tttcaccgcc gtgctgggcc tcgtcgacat
      481 cgggtccgcg cggagtggcc aggcgcaaaa gcagcggatc gaacttcacg ctgatctgct
      541 tcagcagttc acgtcgtcgc ggactgcgtg acgccaggta aatgggagtg gccatcggac
      601 tgggagtggc catcagacgc ggtgatacgg atggcgggcg aggatcgacc aggctcgata
      661 gagctgctcg gccagcacga cgcgggccag tgcatgcggc agggtgaggc tggacaactt
      721 cagttgcatg cgggcttcgc gcttgaaggc atcgtcgagg ccgtccggcc cgccgatcac
      781 gaaggcgacc gactccgcac cgtcgcgcca gcgccgcaac tggtcggcga gttgctgtgt
      841 gctccagtcc ttgccaagtt catccagcgc aacgacggtg ctgccgaccg gcagtgccgc
      901 gcgcaaacgt ttcgcttcgg cggtgcggct gcgttcggct tcgtcggcgc gtccggtgcg
      961 ggcttctgcc ttcaattcct tgagttccac gctgaagtcc gccggaaagc gggccagata
     1021 gtcgtttacc gcctcgtcgg cccaggccgg ctgtttctgc ccgatcgcca cgatcgagat
     1081 cctcattcgg ccgccgccgc cgtcgttcgg tgccgctcag gcggtccggc gcgcaggcgc
     1141 tttgcggctc ttcttacccg ctatcgacgc cggagcggaa gacttgagtc gcacggattt
     1201 gtcgccccag atttcctcca ggttgtagta cgcacggatc gccggctgca gcacatgcac
     1261 gaccgcgtcg ccgcaatcga ccaggaccca ctcgccggtg tctacgccct ccatgctgat
     1321 gacgctgccg ccggcggcct tgaccttctc tgccaccgag gaggcgagcg agcgcgtctg
     1381 ccggttggag gtgccgctgg cgatgacgat gcggtcgaac atgtccgaca gatgcgtcgt
     1441 gttgaagacc ttgatgtcct gtcccttgac gtcctcgagc gcatcgacga tgacgcgttg
     1501 cagcttccta atgtccatgc cgtcccgtgt agaggtgatg cgagtgaata tagtcgagca
     1561 ccggcggcga gaccatcgcg gccagccgtg cagcgtcggc cgatgtcacg ggctgcgcga
     1621 gcaggcgccg aacttccgtg gccgaagcat cgaccggggt catcgcgagg tcgacgatgt
     1681 ggccagcggg gctgtccacg aggtcaccgg gatacttcag gtagcgcgca tctcgcagcc
     1741 gctgcagcgt cggacccagc gaggggattg ctcccgcgcg gcgcgcgatg gccaggtgga
     1801 cgaggtcggg gatgcgctcc cattcgcgcc aggtctcgaa gcgctcgaac tggtcggagc
     1861 ccatgatgag caccagtggc gtgtcccgac ccaccaggcc gcgctgctcg cgcagcgtgt
     1921 cgatcgtgta agtcgggccg ccgcgctcga tctcgctcat gttgagcacc atgcggcggc
     1981 gcgtgaatgg caattcgttg tcgatcgcca ggcgaatcat ccgggcccga tgaaccgcgt
     2041 cggtcagcgc ctctttctgc cagggctggg ccgccggaat cagatggatt tccgcgatcg
     2101 gcaggttggc cagcgcgtcg cgcgcgagct gcaggtgacc gacgtggatc gggtcaaagc
     2161 ttccaccaag caggccgatc ggttccatca ggcgctggcg actatgccca ctcgcgcggc
     2221 cgcaggaagt cggtatacag ccgcgcttcg gcactgccgg gttccggatg ccagtcgtac
     2281 ttccagtgca ccagcggcgg cagcgacatc aggatcgact cggtgcgtcc gcccgacttg
     2341 aggccgaaaa gcgtgccacg gtcgaacacg aggttgaact cgacatagcg gccgcgtcga
     2401 taggcctgga agtcgcgctc gcgaggaccg aagtcgcggt ggcggtggcg ctcgacgatc
     2461 gggacgtagg catcgaggaa cgcatcgccg accgctcgca tcagctcaaa gctcgcgtcg
     2521 aagccgagtt catggaagtc gtcgaagaac acgccaccaa tgccgcgcgg ctcgttgcgg
     2581 ttcttgagga aaaagtattc gtcgcaccat ttcttgaaac gcgggtgttt gtccgcgccg
     2641 aacggcgcca gtgcctgctt gcaggtcgcg tgaaagtggc gcgcgtcttc ctcggtcgcg
     2701 tagtagggcg tcaggtccat gcccccgccg aaccagtaga cgtcgggttc gccggtcttc
     2761 ttcgcaatga agaaccgcac gttcatatgc acggtcggca cgtgaggatt gcgcggatga
     2821 aagaccagcg acacgaccat cgcctcgaag ccgcgacctg caagttcggg gcgctgggct
     2881 gtcgctgagg gcggcagcgt cgagccgatg acgtgcgaga aaccgacgcc accgcgctcg
     2941 agcaggtttc catcctcgat gatgcgcgtg agtccaccgc ctccgagggg ggcgccggtc
     3001 ggtttttccc aggtgtcggc gaggaagggc ctgccgtcga gtttctcgaa ggtaccgacg
     3061 atgtgctgct gc
//
LOCUS       DATIRT010000087         3915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_600472, whole genome shotgun sequence.
ACCESSION   DATIRT010000087 DATIRT010000000
VERSION     DATIRT010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600472"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VLN25_02205"
     CDS             <1..294
                     /locus_tag="VLN25_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09417.1"
                     /translation="WHRYTVGKALFFSLFACWRNRWPFLVLAIALGALIVAVSWVASM
                     LMNVFGVSPQLGSMLLAPIALLLTSVAYSTQYPIYRAVLEPPSVLTAPPVMTA"
     gene            complement(350..2575)
                     /locus_tag="VLN25_02210"
     CDS             complement(350..2575)
                     /locus_tag="VLN25_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HSQ09418.1"
                     /translation="MMNLLQNLNEPQLAAVTLPNQSALILAGAGSGKTRVLTTRIAWL
                     IQTGQVSPQGVLAVTFTNKAAKEMLTRLAAMLPINVRGMWLGTFHGLCNRMLRAHHRD
                     AGLPSTFQILDSADQLGAIKRLLKANNIDDERYPPRNLQMFINSSKEAGLRAKDVEVN
                     DDFNRKFVELYSIYDAQCNREGVCDFAELLLRCYELLDRNEILRQHYQGRFRHILVDE
                     FQDTNKLQYAWLKQLAGGPGGAGNAIFAVGDDDQSIYAFRGANVGNMADFEREFRVRN
                     LIKLEQNYRSVGHILNAANALIANNARRLGKELWTEAGEGEPVRIYEAQTDGYEAAWL
                     VDEVKGLIAEGHTRSEIAVLYRSNAQSRVIEHALFSSAIPYRVYGGLRFFERAEIKHA
                     LAYLRLIENPSDDTAFLRVVNFPPRGIGARSLEQLADAARPGNLSLYAAVGGMTGKAG
                     ANLSAFVKLIETLRFETAQLPLSEIVDAVIQRSGLIPHYQNEREGQERIENLNELVNA
                     ATAFVAEEGYTQDAPATQANEADTASQLPSPLAAFLSHASLEAGDNQAEAGQDALQLM
                     TVHAAKGLEFHAVFITGLEEGLFPHENSLNELDGLEEERRLMYVAITRARRRLYISLA
                     QSRLLHGQTRYGIRSRFLEELPEAAVKWLTPRVAVAAGGRFGQWHDSGVRPASPAVPP
                     PRTASSHGVRVGQAVRHARFGEGVIVNLEGSGDDARAQVNFGPQGVKWLALAVAKLTP
                     A"
     gene            2635..>3915
                     /gene="leuS"
                     /locus_tag="VLN25_02215"
     CDS             2635..>3915
                     /gene="leuS"
                     /locus_tag="VLN25_02215"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012363082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HSQ09419.1"
                     /translation="MEPKYDPQAVEGAAQAHWERTKAYRAVEHVKDAKGQPKPKFYVC
                     SMLPYPSGKLHMGHVRNYTLNDVMYRILRMRGYNVMTPMGWDAFGLPAENAAIDKKVA
                     PAEWTYSNIAEMKAQMQPLGLAFDWSREVATCTPGYYKWNQWMFLKMLEKGIAYRKTQ
                     VVNWDPVDQTVLANEQVVDGRGWRSNAIVEKREIPGYYLGITQYAEELLTDLEKLEWP
                     PQVKAMQENWIGKSVGVNFGFPYELDGEKKLLRVFTTRADTIMGVTFMAIAAEHPLAA
                     NLAQGRPDIAAFIDECRRGSFAEADLATMEKKGIATGFAVTHPLTGAAVPVWIGNYVL
                     MGYGEGAVMGVPAHDERDFAFATKFGLPIPQVIAVPGETFSLGGWQDWYADKTRGVCV
                     NSGKYDGMSYETATDAIAADLNVQGLGDKQIQYRL"
BASE COUNT          701 a         1242 c         1260 g          712 t
ORIGIN      
        1 tggcaccgtt acacggtcgg caaggcgctg ttcttctcgc tgtttgcgtg ctggcgcaat
       61 cgctggccgt ttcttgttct ggcgatcgca ctcggagcgt tgatcgtcgc cgtatcctgg
      121 gttgcatcga tgcttatgaa cgtgttcggc gtctcgccgc aactgggatc gatgctgctc
      181 gcgccgatag cgctgctgct gacgagcgtg gcctactcga cgcaataccc gatctaccgc
      241 gcggtgctcg agccgccgtc agtgctgaca gccccgccag taatgacagc gtagcgcctc
      301 gggtgatcga gacacactgc gcgtggcgca cgtgcacccg cggagcccgc tatgccggcg
      361 tgagcttggc cacggcaagc gccagccact tcacaccctg cggtccgaag ttgacctggg
      421 cgcgcgcatc gtctccgctg ccttccagat tgactatgac gccctcgccg aagcgggcat
      481 gacgcacggc ctggccaaca cggaccccgt gcgatgacgc cgtccgcggc ggcggcacgg
      541 ctggcgacgc agggcgtacc ccgctgtcgt gccattgtcc gaatcgtccg ccggcagcga
      601 ctgccacgcg cggcgtgagc catttcaccg cggcctccgg aagttcctcg aggaagcgtg
      661 atcggatgcc atagcgtgtc tgcccatgca gcaggcggct ctgcgcgagg ctgatgtaga
      721 gccggcggcg cgcccgcgtg atcgcgacat acatcaggcg ccgctcttcc tcgaggccat
      781 ccagttcgtt gaggctgttt tcgtgcggaa acagtccttc ctcgagcccg gtgatgaaca
      841 cggcatggaa ctcgagtccc ttggccgcgt ggaccgtcat cagctgcagc gcatcctggc
      901 cggcctcggc ctggttgtcg ccggcctcga gcgaggcatg cgacaggaac gccgcgagcg
      961 gcgacggcag ctgcgatgca gtgtcggcct cattggcttg tgtggcggga gcgtcctgcg
     1021 tatagccttc ctcggccacg aacgccgttg cggcgttcac cagttcattc aggttttcga
     1081 tgcgctcctg gccttcccgt tcgttctgat agtgcggaat caggccactg cgctggatca
     1141 cggcatcgac tatctccgac agcggcagct gcgcggtttc gaagcgcagc gtctcgatca
     1201 gttttacgaa cgccgagaga ttcgcgcccg ccttgccggt catgccgccg accgccgcat
     1261 aaaggctcag gtttccgggc cgggcggcat cggccagctg ttccagcgac cgagcaccga
     1321 tgccccgggg cggaaaattg accacacgca ggaacgctgt gtcgtcgctc gggttctcga
     1381 tcagtcgaag gtaggcgagc gcgtgcttga tctcggcccg ttcgaaaaag cgcaggccgc
     1441 cgtagacacg gtacggaatg gccgaactga aaagcgcgtg ctcgatgacg cgggactgcg
     1501 cgttcgagcg atacaggacc gcgatctcgg agcgcgtatg cccttcggca atgaggccct
     1561 tgacttcgtc gaccagccag gccgcctcgt aaccgtcggt ctgcgcttcg tagatccgga
     1621 ccggctcgcc ctcgccagcc tcggtccaca gttccttgcc gagcctgcgc gcattgttgg
     1681 caatcagcgc attggccgcg ttcaggatgt ggccgaccga acggtagttc tgctcgagct
     1741 tgatgaggtt gcgcacccgg aactcgcgtt cgaagtcagc catgttgccg acgttcgctc
     1801 cacggaaggc gtaaatcgac tgatcgtcgt cgccgaccgc aaagatggcg ttgccggcac
     1861 ctcccggacc tccggcaagc tgcttcagcc acgcgtactg cagcttgttg gtgtcctgga
     1921 actcgtccac caggatgtgc cgaaaacgtc cctggtagtg ctgccgcagg atctcgttgc
     1981 ggtcgagcaa ctcatagcag cgcagcaaaa gttcggcgaa atcgcagacc ccttcgcggt
     2041 tgcactgtgc gtcgtagatt gagtagagct cgacgaactt gcggttgaaa tcgtcgttga
     2101 cctcgacatc cttagcccgc agcccggcct ccttggagct attgatgaac atctggaggt
     2161 tgcgcggcgg atagcgctcg tcgtcgatgt tgttggcctt cagtaatcgc ttgatcgccc
     2221 ccagttggtc ggccgaatcg aggatctgga acgtcgaagg cagcccggca tcgcggtgat
     2281 gtgcgcgcag catgcgattg cacaagccgt gaaacgtacc gagccacatg ccgcgcacgt
     2341 tgatcggcag catcgcggcc agccgcgtca gcatctcctt ggccgccttg ttggtgaagg
     2401 tcaccgcaag cacaccctgc ggcgagacct ggccggtctg gatcagccag gcgatgcgcg
     2461 tcgtcagcac acgcgtcttg ccgctgccgg caccggcgag gatcagggcg gactggttgg
     2521 gaagagtgac cgcggcgagc tgtggctcgt tgaggttctg cagcagattc atcatcggcc
     2581 gcgagtttag cggcccctat aatcgccctc ttgaatcaaa tacttagaac cgcaatggaa
     2641 ccgaagtacg acccgcaggc ggttgaaggc gcagcgcagg cgcactggga gcgcacgaag
     2701 gcctaccgcg cggtcgaaca cgtgaaggac gcgaagggcc agccgaagcc caagttctac
     2761 gtttgctcga tgctgcccta ccccagcggc aagctccaca tggggcacgt gcgcaactac
     2821 acgctgaacg acgtcatgta ccgcatcctg cggatgcgcg ggtacaacgt gatgacgccg
     2881 atgggctggg acgcgttcgg cctgccggcc gagaacgctg cgatcgacaa gaaggtggca
     2941 ccggccgagt ggacctattc caacatcgcc gagatgaagg cgcagatgca gccgctcggg
     3001 cttgccttcg actggtcgcg tgaagtcgcg acctgcacgc ccggttacta caagtggaac
     3061 cagtggatgt ttttgaagat gctcgagaag ggcatcgcgt accgcaagac ccaggtcgtc
     3121 aactgggatc cggtcgacca gaccgtgctc gcgaacgagc aggtcgtcga cggccgcggc
     3181 tggcgttcca acgccatcgt cgagaagcgc gagatcccgg gttactacct cggcatcacg
     3241 caatacgcgg aagagctcct caccgatctc gaaaagctgg aatggccgcc gcaggtcaag
     3301 gcgatgcagg agaactggat cggaaagagc gtcggcgtca acttcggctt cccgtacgaa
     3361 ctcgacggcg agaagaagct gctgcgcgtc ttcactaccc gcgccgacac gatcatgggc
     3421 gtcaccttca tggcgatcgc cgccgagcac ccgctcgccg cgaacctcgc tcaggggcgc
     3481 cccgacatcg cggcgttcat cgacgagtgc cggcggggca gcttcgccga ggctgacctg
     3541 gcgacgatgg agaagaaggg catcgccacc ggcttcgcag tgacgcatcc gctgaccggc
     3601 gccgccgtgc cggtatggat cggcaactac gtgctgatgg gctacggcga aggcgccgtg
     3661 atgggcgtgc cggcgcacga cgagcgcgac tttgcattcg ccacgaagtt cggtttgccg
     3721 atcccgcagg tgatcgccgt gccgggcgaa acgttttcgc ttggcggctg gcaggattgg
     3781 tatgcggaca agacgcgcgg cgtctgcgtc aactcaggca agtacgacgg catgagttat
     3841 gagacggcca ccgacgcgat cgctgccgac ctgaacgtcc agggcctcgg cgacaagcag
     3901 atccagtacc ggctg
//
LOCUS       DATIRT010000088         2739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1190344, whole genome shotgun sequence.
ACCESSION   DATIRT010000088 DATIRT010000000
VERSION     DATIRT010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190344"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /gene="aroC"
                     /locus_tag="VLN25_02220"
     CDS             <1..987
                     /gene="aroC"
                     /locus_tag="VLN25_02220"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004266572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HSQ09420.1"
                     /translation="LQHDLDRRKPGTSRHVTQRRESDRVEILSGVYEGRTTGTPIALL
                     IRNEDAKSRDYSDIADSFRPGHADYTYWRKYGVRDPRGGGRSSARLTAPMVAAGAVAK
                     KWLHEKYGMRVRGCMQQLGGIEIPFESWEEVERNPFFAPNAGIVADLEIAMDQLRRSG
                     NSVGARLRVTASGVPPGWGAPLFDKLDADIAYAMMGINAVKGVEIGAGFAAVTDLGSQ
                     HGDELLPDGFVGNRAGGVLGGISSGQDIEVSIVVKPTSSIRLPRRTITRAGVATVIET
                     HGRHDPCVGIRATPIAEAMLALVLMDHALMHRAQNADVHHGIAPLAARSPSS"
     gene            987..2051
                     /locus_tag="VLN25_02225"
     CDS             987..2051
                     /locus_tag="VLN25_02225"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSQ09421.1"
                     /translation="MRQDALPQPPDWRLGALFAAYFGLAGLLAPYMPLYFQARGLDAF
                     QIGLLVALGQVMRVIGPNLWGYLADHATRRTPILRSTAVALMLSFCLLYVPGGFAFVF
                     GVMLLLNLFQTAQMPIAEALASARLRGRDDAATRYGRLRLWGSTGFVLVVMAAGPLFD
                     RYGIETALGLGVVLCALLVAVAWQVEEQPMHEAAHERVSVSRRLREPRVRWFLLSAAL
                     MVFAHGALYTYLSIYLAQLGYSKTAIGVFWVLSVVLEIVFFYTQGRWFARFGMYPLLL
                     GSFFVAALRFALIAKLATVWWILAFAQILHAVTFAVHHSASVLMIQRWFPGRAAARGQ
                     AVYISAAYGVGGTAGSLTAA"
     gene            2204..>2739
                     /locus_tag="VLN25_02230"
     CDS             2204..>2739
                     /locus_tag="VLN25_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HSQ09422.1"
                     /translation="MTYLIVGRLLGGVIPCVLTLALSACQTVQTTEAGAVGVDRKQTM
                     TSLVTSQEVEKQAAQQYSQVLAEAKKKDLLNQNPQQVQRVRAVAARLIPQVAVFRGDA
                     VKWNWEVNVLTSKDINAWCMPGGKIAVYTGLIDRLKLTDDELAAVMGHEIAHALREHA
                     RERMGRQMATETATAVGA"
BASE COUNT          423 a          886 c          939 g          491 t
ORIGIN      
        1 ctgcagcacg acctcgaccg acgcaaaccg ggcacctcgc gacacgtgac gcagcggcgc
       61 gaatccgacc gcgtcgagat cctgtccggc gtctacgaag gccgcacgac cggaacgccg
      121 attgcgctgc tgatccgcaa cgaggatgcg aagagccggg actattccga tatcgccgac
      181 tcgtttcgac ccgggcacgc tgactacacg tactggcgca agtacggagt gcgcgatccc
      241 cgcggcggcg gtcgctcgtc ggcccgactg accgccccga tggtggcggc cggcgccgtt
      301 gcgaagaaat ggctgcacga gaagtacggc atgcgagtgc gcggctgcat gcagcagctc
      361 ggcggcatcg agatcccgtt cgagtcgtgg gaggaggtcg aacgcaatcc gttcttcgcg
      421 ccgaacgcgg gcatcgtggc agatctagaa atcgcgatgg accagctgcg gcgaagcggc
      481 aactcggtcg gtgcgcggct gcgcgtgacc gcgagcgggg tgccgccggg gtggggggcg
      541 ccgctcttcg acaagctcga tgccgatatt gcctacgcga tgatggggat caatgcggtc
      601 aagggcgtgg aaatcggcgc cggctttgcc gcggtgaccg acctcggatc gcagcacggt
      661 gatgaactcc tgcccgacgg attcgtcggc aatcgcgccg gcggggtcct cggaggtatt
      721 tcgagcggcc aggacatcga ggtgtcgatc gtggtcaagc cgacatcgag catccgtctg
      781 ccgcgacgga cgatcacgcg agcgggcgtt gccacggtca tcgagaccca cgggcgtcac
      841 gatccctgcg tcggcatccg tgccacgccg atcgccgagg cgatgctggc tctggtgctc
      901 atggaccacg cgctgatgca tcgcgcccag aacgcggacg tccatcacgg catcgcaccg
      961 ctcgccgccc gctctccttc gtcctgatgc ggcaggacgc cttgccgcaa ccgccggact
     1021 ggcggctcgg cgcgctcttt gcggcttatt tcggcctggc gggactgctc gcgccgtaca
     1081 tgccgcttta cttccaggcg cgcggcctgg atgccttcca gatcggtttg ctggtggccc
     1141 tcggccaggt catgcgcgtg atcggtccca acctgtgggg atacctcgcc gatcatgcaa
     1201 cgcgccggac accgatcctg cgcagcaccg ccgttgcgct gatgctgtcg ttctgccttc
     1261 tgtacgtccc cggcggcttt gccttcgtgt tcggtgtgat gctgctgctc aatctcttcc
     1321 agacggccca gatgccgatc gccgaggcgc tggcctccgc ccgcctgcgc ggccgcgacg
     1381 acgctgccac gcgctacggc cgcctgcggc tgtggggctc caccggattc gtcctcgtgg
     1441 tgatggcggc cggtccgctt ttcgatcgtt acggcatcga aaccgcgctg gggctcggcg
     1501 tggtgctgtg cgcgttgctg gtggccgtgg cctggcaggt ggaagagcag ccgatgcacg
     1561 aggcagcgca cgagagggtg tccgtcagcc gccgcctgcg cgaacctcgc gtccgctggt
     1621 tcctgctgtc ggcggcgctg atggtgttcg cccatggggc gctgtacacc tatctgtcga
     1681 tctacctggc gcaactcggt tacagcaaaa cggcgatcgg cgtgttctgg gtgctgagcg
     1741 tcgtactcga gattgtgttt ttctacaccc agggtcgctg gttcgcgcgt ttcggcatgt
     1801 atccgctgct gctggggagc ttcttcgtcg ctgcgctgcg gtttgcattg atcgcgaagc
     1861 tcgctaccgt atggtggatc ctggcattcg cgcaaatcct gcatgcggtc acgttcgcgg
     1921 tacatcacag cgcctcggtc ctgatgatcc agcgctggtt ccctggccga gcggcggctc
     1981 gaggccaggc cgtgtatatc agcgctgcct acggcgtggg cggcacggcg ggtagcctga
     2041 ccgcggcata attgtggacg ctggccggcc cggcatgggc gttcggctcc ggcagcgccg
     2101 ccgcgctgct cggcgcctgg gcggcccgcc aggtgcgccg gcatgacgcg acgctggacg
     2161 gcccacatgc ggttgccttg acttcaaaaa ggaacatcga ctcatgactt acctcatcgt
     2221 tggccgcctg ctgggcggcg tgatcccgtg tgtgcttacc ctggcactct cggcctgcca
     2281 gaccgtgcag acgaccgaag cgggtgcggt cggcgtcgat cgcaagcaga cgatgacgtc
     2341 gctggtcacc agccaggagg tggagaaaca ggcggcgcag cagtattcgc aggtgctagc
     2401 ggaggcgaag aagaaggacc tgctgaatca gaacccgcaa caggtccagc gcgtgcgcgc
     2461 ggtcgctgcc aggctgatcc cacaggtcgc ggtgtttcgc ggcgacgcgg tgaagtggaa
     2521 ctgggaagtg aatgtcctga cgtccaagga catcaatgcc tggtgcatgc cgggcggcaa
     2581 gatcgccgtc tacaccgggc tcatcgaccg attgaagctg accgacgatg aactcgcggc
     2641 cgtcatgggc cacgagatcg cgcatgcctt gcgcgagcac gcgcgcgagc gcatgggccg
     2701 gcagatggcc accgaaaccg ccacggcggt cggcgccat
//
LOCUS       DATIRT010000089         1832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_305582, whole genome shotgun sequence.
ACCESSION   DATIRT010000089 DATIRT010000000
VERSION     DATIRT010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305582"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VLN25_02235"
     CDS             complement(<1..351)
                     /locus_tag="VLN25_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HSQ09423.1"
                     /translation="MTTLIDAQANLRTLRDVLRYAVTRFNEAQLSFGHGQADAFEEAA
                     FIVMRTLKLPTERLDFFADAYLTHAEIHELLQAIDRRVKKRLPAAYLLNEAWLRGYKF
                     YVDERVIVPRSFIGE"
     gene            complement(348..1490)
                     /gene="dapE"
                     /locus_tag="VLN25_02240"
     CDS             complement(348..1490)
                     /gene="dapE"
                     /locus_tag="VLN25_02240"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HSQ09424.1"
                     /translation="MNPTLELVQDLIARRSVTPEDGGCQELLATRLAASGFTAETISG
                     NGVTNTWLRRGTAAPVFVFAGHTDVVPTGPVDQWLSDPFTPTLRDGDLVGRGAADMKS
                     SIAAFVVAAEEFVRAHPGHPGSIGLLLTSDEEGPATHGTTLVVEALRKRGEAIDYCIV
                     GEPTSVVRLGDTIKNGRRGSLSGRLTVRGVQGHVAYPHLARNPVHLLAPALAELAAVE
                     WDRGDEFFPPTTFQVSNIHAGTGVGNVIPGSCVVDFNFRFAPASTAASLQQHVEKLLA
                     RHGVDFAIDWTLGAAPFLTPRGSLSQAISAAIEQITGAQPELSTTGGTSDGRFIATFC
                     PQVIEFGPPNATIHQINERISVADLEPLKNIYFGTLTRLLAPTGKQ"
     gene            complement(1621..>1832)
                     /locus_tag="VLN25_02245"
     CDS             complement(1621..>1832)
                     /locus_tag="VLN25_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HSQ09425.1"
                     /translation="APESISFEQSLYSLIQGEQITREDALAAADSQNNLLWFINNAGK
                     SRSQLPKERKEEGSDASFSEFTLNI"
BASE COUNT          313 a          568 c          639 g          312 t
ORIGIN      
        1 ttcgccgatg aacgagcgcg gcacgatcac gcgctcgtcg acatagaact tgtagcctcg
       61 cagccaggct tcgttgagca ggtacgccgc cggcaagcgc ttcttcacgc gacggtcgat
      121 ggcttgcagc agttcgtgga tctcggcgtg ggtcaggtag gcatcggcga agaagtccag
      181 ccgctcggtc ggcagcttga gagtgcgcat cacgatgaag gccgcctcct cgaacgcatc
      241 cgcctggccg tggccgaacg aaagctgagc ctcgttgaag cgggtcaccg cgtagcgcag
      301 cacgtcgcgc agcgtgcgca gattggcctg cgcgtcgatc agcgtggtca ttgttttcct
      361 gtgggtgcaa gcaggcgcgt cagcgtgccg aagtaaatgt tcttgagggg ttcgagatcg
      421 gcgacagaga tgcgctcgtt gatctggtgg atggtcgcat tcggtggacc gaactcgatc
      481 acctgcgggc agaaagtcgc gatgaaccgg ccatcggagg tgccgccggt ggtcgagagt
      541 tcgggctgcg cgcccgtgat ctgctcgatc gcggcgctga ttgcctgtga tagcgagcca
      601 cgcggcgtca ggaagggcgc agccccgagt gtccagtcga tcgcaaagtc aaccccgtga
      661 cgcgccagca gcttttcgac gtgctgctgc agcgacgcgg cggtcgatgc cggcgcgaac
      721 cggaaattga agtcgaccac gcaggaaccg gggatcacgt taccgacgcc ggtgccagca
      781 tgaatgttcg agacctgaaa cgtcgtcggc gggaagaact cgtcaccgcg atcccattcg
      841 accgcggcca gttccgccag ggccggtgca agcaggtgca cggggttgcg cgcaaggtgc
      901 ggataggcga catgcccctg gactccacgc accgtcagtc gaccggacag cgatccgcgg
      961 cgcccgttct tgatcgtgtc gccgagccgc acgaccgagg tcggctcgcc gacgatgcag
     1021 taatcgatcg cctcgccgcg cttgcgcagt gcctcgacga cgagcgtggt gccgtgggtg
     1081 gccggtcctt cttcgtcaga ggtcagcagc aggccgatcg aaccgggatg gccaggatgc
     1141 gcacgaacga attcctcagc ggcgacgacg aaggcggcga tcgaactctt catgtcggcc
     1201 gccccgcgcc cgaccaggtc gccgtcgcgc agtgtcggcg tgaacgggtc cgagagccac
     1261 tggtcgacgg gaccggtggg cacgacatcg gtatggccgg cgaacacgaa caccggagcg
     1321 gccgtgccgc gccgcagcca ggtgttcgtc acgccgttgc ctgaaattgt ctcggccgtg
     1381 aagcccgagg cggcgagtcg tgtcgccagc agttcctggc agccgccgtc ctccggcgtg
     1441 acggatcgac gggcgatcag gtcctgcaca agttcgagag tgggattcac gggcggcctg
     1501 ggtgctgcgc gctccgtgac tcgcccatgc ggcgacccac cggtctgatg cgcgcgcctt
     1561 gcgtgccagg ggcggcaggc taggcgcgca gattgtagcg gcagcgccga gcggcgcgac
     1621 ctagatgttc agcgtgaact ccgaaaacga ggcgtccgag ccttcttcct tgcgctcctt
     1681 gggcaactgg gagcgggact tgccggcatt gttgatgaac cagagcaggt tgttctgcga
     1741 atcggccgcg gcgagcgcgt cctcgcgcgt gatctgctcg ccctggatca gcgagtacag
     1801 gctttgctcg aacgagatgc tctcgggcgc ca
//
LOCUS       DATIRT010000090         3498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_895650, whole genome shotgun sequence.
ACCESSION   DATIRT010000090 DATIRT010000000
VERSION     DATIRT010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895650"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /gene="pdxA"
                     /locus_tag="VLN25_02250"
     CDS             <1..677
                     /gene="pdxA"
                     /locus_tag="VLN25_02250"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004530907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HSQ09426.1"
                     /translation="GRYDAIVTAPVQKSVINDAGIPFSGHTEYLAERTSTAQVVMMLA
                     GATPHGPLRVALATTHLPLARVSAALSIDTLAGTLRILDHDLRRRFGLAHPRIAVAGL
                     NPHAGEAGHLGREEIEVITPAIERVRGEGIDARGPLPADTLFTPAQLQHCDAVLAMFH
                     DQGLPVLKYATFGQGVNITLGLPFVRTSVDHGTALDLAGTGRAHAGSLRVALELAIEL
                     VGRQRP"
     gene            718..1533
                     /gene="rsmA"
                     /locus_tag="VLN25_02255"
     CDS             718..1533
                     /gene="rsmA"
                     /locus_tag="VLN25_02255"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016475175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HSQ09427.1"
                     /translation="MSTAGLQHRARKRFGQNFLHDPRVIARIIDAIDPHPGQTIVEIG
                     PGQAALTRALVERAGHVTALEIDRDLASWLRKEFTPQQLTLIEADALKFDWSSLPGSL
                     RLVGNLPYNISSPLLFALAPIAERVLDQHFMLQKEVVDRMVASPGSRTYGRLSAMLQF
                     RYRLTKLFDVPRGAFNPPPQVTSSIVRMQPLPIAELPLIDPAIYGRVVATAFGQRRKT
                     LRNALATLMSAEDITRAGVDPQARAEVLSVADFVRLSDAYIRARPAPLTDGTG"
     gene            complement(1514..1900)
                     /gene="gloA"
                     /locus_tag="VLN25_02260"
     CDS             complement(1514..1900)
                     /gene="gloA"
                     /locus_tag="VLN25_02260"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HSQ09428.1"
                     /translation="MRILHTMLRVGDLQRSIDFYTKVLGMQLLRTNDRPEQKYTLAFV
                     GYGDEREHAVLELTYNYGVDRYDLGTAYGHIAIAVPDAYVACERIRTSGGNVTRAAGP
                     VQGGTTVIAFVTDPDGYKIELIQSRQ"
     gene            complement(1914..2723)
                     /locus_tag="VLN25_02265"
     CDS             complement(1914..2723)
                     /locus_tag="VLN25_02265"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HSQ09429.1"
                     /translation="MKRLKVTSAEQLSLVLEEGDAASVPSGSRGNETPLRRVQVGPAL
                     LTYRLRRARRRSIGFQIDDQGLTVSAPRLVPLREIEAAIAEKQRWITNKQREWQEWRA
                     RRRLPQVQFVQGAALPYLGGELVLRLGASRVVTTREAGELHVALPADALEQRVRDTVQ
                     AWLQAEARRVFQDRIERFADRIPPRYAGWSLSSARTQWGSCTHNGRVRLSWRLIHFSL
                     PVIDYVVAHELAHLAELNHGPRFWNTVAQLLPGFETARDEIRRVDMAALPL"
     gene            complement(2720..>3498)
                     /locus_tag="VLN25_02270"
     CDS             complement(2720..>3498)
                     /locus_tag="VLN25_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HSQ09430.1"
                     /translation="RSALYLLFLIVVVIPWAFVVLSGWLFPVHTRYYLCACYTKMAIW
                     GARVICGIEWKVEGWDNLPDGPAILLPKHKSSWETLWLPSVMPRDLTFVYKRELNWLP
                     FFGWGLASAQMISIDRRKGQNAFEQVVEQGIDRLGRGWWIVIFPEGTRTRPGSTRRFK
                     TGGARLAVSTGAFAVPIAVNSGELWPRNAFIRRPGLITVSIGPPIDPHGKSPDEVGAA
                     VESWIETEMRRLAPHRYSGPYVPARVPAAARVPTADAGPA"
BASE COUNT          600 a         1218 c         1114 g          566 t
ORIGIN      
        1 cgggacgcta cgacgcgatc gtcaccgcgc cagtgcagaa aagcgtcatc aacgacgcgg
       61 gcattccgtt ctcgggacac accgagtacc tggccgagcg gacatcgacg gcacaggtag
      121 tgatgatgct ggccggcgca accccgcacg gcccgctacg cgtcgcgctt gccaccaccc
      181 atctgccgct ggcccgtgtc agcgcggctc tgtcgatcga caccctggcc ggaacgctgc
      241 gcatcctcga tcacgacctg cgccgtcgat tcggcctggc gcatccgcgc attgcagtgg
      301 cgggcctcaa tccgcatgcc ggcgaggccg ggcacctggg gcgcgaagag atcgaggtca
      361 tcacgccggc gatcgaacgc gttcgcggcg aaggcatcga tgcgcggggc ccgctgccgg
      421 ccgacacgct gtttactccg gctcaattgc agcactgcga cgcggtgctc gcgatgttcc
      481 atgaccaggg gctgccggtc ctcaagtacg ccaccttcgg acagggcgtc aacatcacgc
      541 tcggcctgcc gtttgtgcgg acctcggtcg accacggcac ggcgctggat cttgccggga
      601 ccggccgcgc acatgccggc agcctgcgcg tagcgctgga actggcgatc gagctggtcg
      661 ggcggcagcg gccatgaagg caccgccgcg acatcgcgcg gcacgcctgc attcgcgatg
      721 agtacggccg ggctgcagca ccgcgcgcgc aagcgcttcg gccagaactt cctgcacgac
      781 ccgcgcgtca tcgcacgcat catcgacgcg atcgatccgc accccggcca gaccatcgtc
      841 gagatcgggc cgggtcaggc cgcgctgacg cgagcactgg tcgaacgcgc cgggcatgtc
      901 acggcgctcg aaatcgatcg ggacctggcg tcctggttgc gcaaagaatt caccccgcag
      961 caattgacac tgatcgaggc cgacgcattg aagttcgact ggagcagcct cccgggttcg
     1021 ctgcgcctgg tcggaaacct gccctacaac atctcgtcgc cgctgctgtt cgcgcttgcg
     1081 ccgatcgccg aacgcgtgct cgaccagcac ttcatgctgc aaaaggaagt ggtcgaccgg
     1141 atggtcgcct ccccgggcag ccgcacctac ggtcggctgt cggcgatgct gcagttccgc
     1201 taccgcctga ccaagctttt cgatgttccg cgcggtgcct tcaatccgcc accgcaggtg
     1261 acctccagca tcgtccgcat gcagcccctg cccatcgcag agctgcctct gatcgatccc
     1321 gcgatctacg ggcgcgtggt cgccacagcc ttcgggcagc gtcgcaagac cctgcgcaac
     1381 gcactcgcca cgctgatgag cgccgaggac atcactcgcg caggcgtcga tccgcaggcg
     1441 cgcgcggaag tgctgtcggt cgcggacttc gtgcggttga gcgacgcgta catccgcgcc
     1501 cggcccgcgc ctcttactga cgggactgga tgagttcgat cttgtagccg tccgggtccg
     1561 tcacgaacgc aatcaccgta gttccgccct gcacgggtcc ggcggcccgg gtcacgttgc
     1621 cgccggacgt ccggatgcgt tcgcacgcta cgtaggcatc gggcaccgcg atcgcaatgt
     1681 ggccataagc ggtgccgagg tcgtagcgat ccacgccata gttgtaggtg agctcgagta
     1741 ccgcatgctc gcgttcgtcg ccgtagccga cgaaggccag cgtgtacttc tgctccggac
     1801 ggtcgttcgt gcgcagcaac tgcatgccga gcaccttcgt gtagaagtca atcgagcgct
     1861 gcaggtcgcc gacgcgcagc atggtgtgaa ggattcgcat caggtctccg aagttagagg
     1921 ggcagggctg ccatgtcgac gcgccggatc tcgtcgcgcg cagtctcgaa acccggcagc
     1981 agttgcgcca ccgtgttcca gaagcgcgga ccgtgattca gctccgccag gtgcgcgagt
     2041 tcatgcgcca cgacgtagtc gatcaccggc agcgagaaat ggatcaggcg ccagctcagc
     2101 cggacacgtc cgttgtgcgt gcacgagccc cattgtgttc gcgcggacga cagtgaccag
     2161 ccggcgtagc gggggggaat gcgatcggca aagcgctcga tgcgatcctg gaacacgcgc
     2221 cttgcctccg cctgcagcca tgcctgcacc gtgtcgcgca ctcgctgctc caaggcgtca
     2281 gctgggagcg cgacatgcag ttcgccggcc tcgcgggtgg tgacgacgcg gctggcgccg
     2341 aggcgcagca ccagctcgcc gccgagatag ggcaaggccg cgccctgtac gaactgcacc
     2401 tgcggcagcc ggcgccgcgc acgccactcc tgccactcac gctgcttgtt cgtgatccag
     2461 cgctgctttt cggcgatcgc tgcttcgatc tcgcgcagcg gcaccaagcg cggtgcactg
     2521 acggtcagcc cctggtcatc gatctggaaa ccgatcgaac ggcgacgcgc ccgccgcaaa
     2581 cggtaggtca gcagtgcggg cccgacctgt acccggcgca atggagtttc attccccctc
     2641 gacccggacg gaacggatgc ggcatcgccc tcttccagaa cgagcgatag ctgctcagcc
     2701 gatgtgacct tgagtcgctt catgcgggac ccgcatccgc cgtggggacg cgcgccgccg
     2761 ccggcactct ggccggtaca tacgggccgc tgtagcgatg cggagccagc cgccgcatct
     2821 cggtttctat ccatgactcg acggcggctc ccacttcatc agggctcttg ccatgcggat
     2881 cgatgggagg gccgatcgac accgtgatca gtcctggccg cctgatgaat gcgttccgcg
     2941 gccacagttc gccggagttc accgcgatcg gcacggcaaa cgctcccgtg ctgaccgcga
     3001 gccgcgcgcc acctgtcttg aagcgccgcg tcgacccggg ccgcgtgcgc gtgccctcgg
     3061 gaaagatcac gatccaccag ccacgcccga ggcggtcgat gccctgctcg accacctgct
     3121 cgaacgcgtt ctgccccttg cgccgatcga tgctgatcat ctgggccgac gccagtcccc
     3181 agccgaaaaa cggcagccag ttgagttcac gcttgtagac gaaggtcagg tcgcgaggca
     3241 tcacggaggg cagccagagc gtctcccacg atgacttgtg cttcggcagc aggatcgccg
     3301 ggccgtcagg caagttgtcc cagccttcga ccttccactc gatgccgcag atgacgcgcg
     3361 caccccagat cgccattttc gtgtagcacg cgcacaggta gtagcgcgta tgcaccggga
     3421 acagccaacc cgacagcacg acgaaggccc acgggatgac gacgacaatc aggaagagca
     3481 gatacagcgc cgagcgca
//
LOCUS       DATIRT010000091         6476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_601257, whole genome shotgun sequence.
ACCESSION   DATIRT010000091 DATIRT010000000
VERSION     DATIRT010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6476
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601257"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /gene="gltA"
                     /locus_tag="VLN25_02275"
     CDS             <1..1192
                     /gene="gltA"
                     /locus_tag="VLN25_02275"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HSQ09431.1"
                     /translation="KSGKFTYDPGFLSTASCNSTITYIDGDKGELLYRGYPIEQLATK
                     CDYLETCYLLLYGELPNPKQKEDFVKRVTNHTMVNEQMQFFMRGFRRDAHPMAVMTGL
                     VGALSAFYPDSMNLNDPWQREVSAIRLIAKMPTLVAMAYKYTVGQPYMYPRNDLSYAG
                     NFMRMMFATPCEDYKVNDVLVRAMDRIFILHADHEQNASTSTVRLCASSGTNPFAAIA
                     AGVACLWGPAHGGANEACLNMLYDIQRNGGVAKIGEFIDKVKDKNSTVKLMGFGHRVY
                     KNYDPRAKLMRETCNEVLDSLGLHDDPLFKLAMSLEKIALEDDYFVSRKLYPNVDYYS
                     GIVQKAIGIPVPLFTAIFALARTVGWIAQLNEMIADPEYKIGRPRQLFVGATARDVKP
                     IGQR"
     gene            complement(1398..2105)
                     /locus_tag="VLN25_02280"
     CDS             complement(1398..2105)
                     /locus_tag="VLN25_02280"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HSQ09432.1"
                     /translation="MKILRSDLAHGHELEGAVVVIDVLRAFTTAAYAFAAGARAMIAA
                     DSLEGVCRLRSRYAASMTMGALGGGRPVPGLDFGNSPAEIVGRDLSGVTLIQFTAGGV
                     KGLVDCDHATEVLAGSLVCARSTARYIRTLAPSTVTLVITGLWTDRDGDEDHACADLI
                     EAYLLGRSPDRAHYEQRVRQSDFGRRFGTAAYPHLPAADLDLCAAADRFGFAMPMHRT
                     DHGLVIEPVALAEMPAG"
     gene            complement(2129..4372)
                     /locus_tag="VLN25_02285"
     CDS             complement(2129..4372)
                     /locus_tag="VLN25_02285"
                     /inference="COORDINATES: protein motif:HMM:NF024763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HSQ09433.1"
                     /translation="MDMAAKFQDQTVFHMTGKRSGDGLAALQAGFRPALLAAYRDLTR
                     LRYDYPVVLLERDAGTDYVRSLSSIVGELIADIAPRGIEGERVRKHLLRLEREIRVLL
                     AAGTAGTLTELWPLAAVKAAGRDDNVREILTRAARSLEVDGEVLNCEGDLAARFLRRA
                     WRNVQSERSQQFRTLVDHLVRKLSDILRAAHVHSQAGQQPAALQSGFGALHSDIFDFS
                     AMSRLVSRNVPQDELPALRRHRIEWALSILRSQPFYPDPKGAGAETVYEFAFSDCATA
                     IEAHRTRLPKLVEVVKAIAVAELEARGGYVEAKHDAFFESYDEHALTADDLAQFPDYL
                     VCIPPDRNAAPENAALLEMLSAGMPVKVLVQLTDLLEDAAIGQGHFAFGVRSARLATT
                     AMGLGGMFVLQSTSSNLYALRERVRCGMGCRGPALFSVFTGSPDSASELSAYLTTAAA
                     MKSRAFPAFTYDANAGSNWATRFSFENNRNPEDDWPVEDFEYADETLQRIQERVHFTY
                     ADFVLCDRRHAHHFAVVPRERWTTSMLPAADWLTLTEREAAERVPYVLAVDGSDQLHR
                     VIVDSRLLQSTRRCLLLWHRLQEHGGIHNSHADQALAREKVTWEAQKQQEIEALKQAA
                     PTAASTATTTAAPAATAESATAPPPAAIASEETAEVAAAPPSDEPWIETIRCSSCNEC
                     QNINDKLFAYNDNKQAYIKDVNAGTYRHMIEAAEACQVAIIHPGKPKNPKEPGLEELL
                     ERAKPFL"
     gene            complement(4401..5387)
                     /locus_tag="VLN25_02290"
     CDS             complement(4401..5387)
                     /locus_tag="VLN25_02290"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HSQ09434.1"
                     /translation="MGSALSQCIIKLHEERHTLEDYQGGVPRWCSGCGDNAILAAVQR
                     LCRDEDLAPERTVFVSGIGCSSRFPHYMKTYGFHGIHGRAFPLAEGVKMARPDLNVFV
                     NTGDGDCCSIGAAHWIHAIRYNMNLTVILHDNQVYGLTKKQASPTSPVGFKSNTTPRG
                     SVLEPLNPLSVTLGVQNASFVAQGVDWIPELLYDIISKAFKHRGFSFVRIIQRCPEWL
                     PTTFEPWLHDPGKTLLLTHENGLQPSADTSRIYKNQREHDPLNIHQAREIASGTDPIP
                     VGILYHDPSVPCYDDLRGAGQPRTTEAIRAGLNKEFDKFTIWPDEERELRAA"
     gene            complement(5391..>6476)
                     /locus_tag="VLN25_02295"
     CDS             complement(5391..>6476)
                     /locus_tag="VLN25_02295"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09435.1"
                     /translation="ASHYLSECFERVGGIVHQAEDEIAACAFAIGASYAGRCAVTITS
                     GPGYSLKQEGIGLAVMAEIPLVVVNVQRGGPSTGQPTKVEQADLLTAIYGSHGDAPKV
                     VIAATGIEDCFYSMITARKIAETFNMVVVVLTDASLATAQQPFARPDFSEDWLAPPID
                     QSPLPPGAKPYDWDKMTGISRRFSPGQPNGMHTVTGLAHDRASKVAYDPAINEEGLRA
                     RSLKLAALQKTLKTPSVYGDPEGDLMVVGWGSTQGIIEEAVQRVRDEGLKVSALQMRF
                     IQPMPSGIATILKRFRKVMTVEGTWADRPAEDELIDEENRRYAPLAMMLRARTLVDVD
                     CWTEARGQPIKPGLVVNAIRAKLSEGK"
BASE COUNT         1068 a         2064 c         2169 g         1175 t
ORIGIN      
        1 caagtcgggc aagttcacct atgacccggg cttcctgtcg acggcgtcgt gcaactcgac
       61 catcacctac atcgacggtg acaagggcga actgctgtac cgcggctacc cgatcgagca
      121 gctggcgacc aagtgcgact acctcgagac ctgctacctg ctgctctacg gcgagttgcc
      181 gaatccgaag cagaaggagg acttcgtcaa gcgcgtgacc aaccacacga tggtcaacga
      241 gcagatgcag tttttcatgc gcggtttccg ccgcgatgcg cacccgatgg cggtcatgac
      301 cggtctggtc ggtgcgctgt cggcgttcta cccggactcg atgaacctga acgacccctg
      361 gcagcgcgag gtctcggcga tccgcctgat cgccaagatg ccgacgctgg tcgcgatggc
      421 ctacaagtac accgtgggcc agccgtacat gtacccgcgg aacgatcttt cgtacgccgg
      481 caacttcatg cgcatgatgt ttgccacacc atgcgaggac tacaaggtca atgatgtgct
      541 ggtgcgcgcg atggatcgca ttttcatcct gcacgcggac cacgagcaga acgcgtcgac
      601 ctcgaccgtg cgcctgtgcg cgtcgtccgg caccaacccg ttcgcggcga tcgcggccgg
      661 cgtggcgtgc ctgtggggtc cggcgcacgg cggtgccaac gaggcctgcc tcaacatgct
      721 ctacgacatc cagagaaacg gtggcgtggc caagatcggc gagttcatcg acaaggtcaa
      781 ggacaagaac agcaccgtca agctgatggg cttcggtcat cgcgtctaca agaattacga
      841 tccccgcgcg aagctgatgc gcgagacctg caacgaggta ctggactcgc tcggcctgca
      901 cgacgacccg ctgttcaagc tggcgatgtc gctcgagaag atcgcacttg aggacgacta
      961 cttcgtcagc cgcaagctct atccgaacgt cgattactac tcaggtatcg tgcagaaggc
     1021 gatcggcatc ccggtgccgc tgttcaccgc catctttgcg ctggcccgca ccgtgggctg
     1081 gattgcgcag ctgaacgaga tgatcgcgga tcctgagtac aagatcgggc gtccccggca
     1141 actctttgtc ggcgcaaccg cgcgcgacgt gaagcccatc gggcagcgat agcacgcagg
     1201 ttcgccacga aaaacggcgc ccgggggcgc cgtttttcat gacctcaacg cggtcgcaac
     1261 gcgcgactgc cgaggccggg cagcactcgc aggaagttga gtcccccaga ggcggccgac
     1321 cgtccggggc cctcggcatt ggtgggttct tttggtcgag gcctattgtt tccgatctgc
     1381 ggtgacacgg cagatcgtca gcctgccggc atttcggcca gcgccaccgg ttcgatgacc
     1441 agtccgtgat cggtccggtg catcggcatg gcaaagccga agcgatcggc ggcagcgcag
     1501 aggtcgagat cggcagcagg caggtgaggg tacgccgcgg tgccgaagcg ccggccgaag
     1561 tcggactgtc gtacgcgctg ctcgtagtgc gcccggtcgg gggaccgccc gagcaggtag
     1621 gcctcgatca ggtcggcgca ggcgtgatcc tcgtcgccat cgcggtcggt ccacaggccg
     1681 gtgatcacca gtgttacggt cgacggtgcc agtgtgcgga tgtagcgcgc tgtcgagcga
     1741 gcgcagacga ggctgccagc gagcacttcg gtcgcgtggt cgcagtcgac cagccccttg
     1801 actccgccgg cggtgaactg gatcagggtg acgcccgaga ggtcacggcc gacgatttcg
     1861 gccggcgagt tcccgaagtc cagcccgggg accggtcggc ctcctccgag cgcccccatc
     1921 gtcatcgacg ctgcgtaacg gctgcgcaac cggcagacgc cttcgagcga atcggcggcg
     1981 atcatcgcgc gcgctccggc ggcaaacgcg taggccgccg tcgtgaaggc gcgcagcacg
     2041 tcgatgacga ccacggcacc ttccagctcg tggccgtgcg caaggtccga gcgcaggatc
     2101 ttcatcgccg gctcgatcta acgcgtggct acaggaacgg cttcgcgcgc tccagcagtt
     2161 cctcgagtcc gggttccttc gggttcttcg gtttgcctgg atgaatgatc gcgacctggc
     2221 aagcctcggc cgcttcaatc atgtggcgat aggtgcctgc gttcacgtcc ttgatgtagg
     2281 cctgcttgtt gtcgttgtac gcaaacagct tgtcgttgat gttctggcac tcgttgcagc
     2341 tcgagcagcg gatggtctcg atccacggct cgtccgacgg cggggcagcg gcaacttcgg
     2401 ccgtttcttc cgatgcgatt gccgcgggcg gtggggcggt tgcggactct gccgtcgcgg
     2461 cgggtgcggc cgttgtagtg gccgtcgaag cggcggttgg ggcggcctgt ttcagcgcct
     2521 cgatttcctg ctgcttctgc gcctcccacg tcaccttctc gcgcgccagg gcctggtctg
     2581 cgtgcgagtt gtggatgcct ccatgttcct gcaggcgatg ccacagcaac aggcaccggc
     2641 gcgtcgactg cagcagtcgc gagtcgacga tcacgcgatg cagctgatcg gaaccgtcaa
     2701 cggcaaggac gtacgggacg cgctcggcgg cttcgcgttc ggtcagcgtc agccagtcgg
     2761 ccgccggcag catggacgtg gtccagcgtt cgcgtggaac gaccgcaaaa tggtgtgcgt
     2821 gacggcggtc gcacagcacg aagtcggcat acgtgaagtg aacgcgctcc tgtatgcgct
     2881 gcagggtctc gtcggcgtac tcgaagtcct cgaccggcca gtcgtcctcg ggattgcggt
     2941 tgttttcgaa ggaaaaccgg gtcgcccagt tgctgccggc gttcgcgtcg taggtgaagg
     3001 cgggaaaggc acgcgacttc atcgccgcgg ctgtcgtgag atacgcggac agttcgctgg
     3061 cgctgtcggg cgaacccgtg aacacgctga acaacgccgg accgcggcaa cccatgccac
     3121 accgaacgcg ctcgcgcagc gcatacaagt tcgaactcgt cgactggagc acgaacatgc
     3181 cgcccaggcc catcgcggtc gtggccagcc gggcgctgcg cacgccgaag gcaaagtggc
     3241 cctggccgat ggcggcgtct tccagcaggt cggtgagttg caccaggacc ttgaccggca
     3301 tgccggcgga aagcatctcc agcagcgcgg cattctcggg cgccgcattg cggtccggcg
     3361 gaatgcagac caggtagtcc gggaactgcg cgaggtcgtc ggctgtcagc gcgtgctcgt
     3421 catagctctc gaagaatgcg tcgtgcttgg cctcgacata gccgccgcgc gcctcgagtt
     3481 ccgccacggc gatggctttc accacctcga ccagcttggg cagccgcgtg cgatgcgcct
     3541 cgatcgccgt tgcgcagtcg ctgaatgcga actcgtagac cgtttctgcg cccgcgccct
     3601 tggggtcggg atagaagggc tgactgcgca ggatcgaaag tgcccactcg atccgatggc
     3661 gcctgagcgc aggcagctcg tcctgcggca cattgcgcga aaccaggcgc gacatggcgg
     3721 agaagtcgaa gatgtccgaa tgcagtgctc cgaacccgga ttgcagcgcg gccggttgct
     3781 ggcccgcctg cgagtggaca tgggcggcgc gaaggatgtc cgagagcttg cgcaccagat
     3841 gatcgaccag cgtgcggaac tgctgcgacc gttcggattg cacgttgcgc cacgcgcgac
     3901 ggaggaagcg cgccgcgaga tcgccttcgc agttgagcac ctcgccgtcg acctccagcg
     3961 accgcgcggc ccgcgtgagg atctcgcgca cgttgtcgtc acggccggcg gccttgactg
     4021 cagccagcgg ccatagctcg gtcagcgtgc cggccgtgcc ggcggcaagc agcacccgga
     4081 tctcgcgttc gaggcgcagc aggtgcttgc gcacgcgttc gccctcgatg ccgcgcggcg
     4141 cgatgtcggc gatcaactcg cccacgatcg acgagagcga cctgacgtag tcggtgcccg
     4201 catcgcgctc gagcagcacg accggatagt cgtagcgcag gcgcgtcagg tcgcgatacg
     4261 cggccagcag ggcgggccgg aatccggcct gcaacgcggc gagtccgtcg cccgagcgtt
     4321 tgccggtcat gtggaaaacg gtctggtcct ggaacttggc ggccatgtcc atggcggctt
     4381 acctcgattt tcggcggtgg tcaggcggcg cgcagctcgc gctcctcgtc cggccagatc
     4441 gtgaacttgt cgaactcctt gttgagtccg gcacgaatgg cttcggtggt gcgcggttgt
     4501 ccggctccgc gcaaatcgtc gtagcaaggt acgctcgggt cgtggtacag gatgccgact
     4561 ggaatcggat cggtgcccga cgcgatctcg cgcgcctgat ggatgttcag cgggtcgtgt
     4621 tcgcgctggt tcttgtaaat ccggctggtg tcggcactcg gctgcaggcc gttttcgtgc
     4681 gtgagcagca gcgtcttgcc cggatcatgc agccacggtt cgaacgtcgt cggcagccac
     4741 tcggggcagc gctggatgat gcgcacgaac gagaaaccgc gatgcttgaa ggccttgctg
     4801 atgatgtcgt agaggagttc cggaatccag tccacgccct gcgccacgaa cgaggcgttc
     4861 tgaactccca gcgtcacgga gagcgggttc agtggctcga gcacggagcc gcgcggcgtg
     4921 gtattgctct tgaatccgac cggcgaggtg ggcgaagcct gcttcttggt gagcccgtac
     4981 acctggttgt cgtgcaggat caccgtcagg ttcatgttgt agcgaatggc gtggatccaa
     5041 tgcgccgcgc cgatgctgca gcagtcgcca tccccggtgt tgacgaagac attcaggtcg
     5101 ggacgcgcca tttttacgcc ttcggcgagc gggaaggcgc ggccgtggat gccgtggaag
     5161 ccgtaggtct tcatgtaatg cgggaagcgg ctcgagcatc cgatgccgga tacaaagacc
     5221 gtgcgctcgg gcgcaaggtc ctcgtcgcgg cacaggcgct gtacggccgc caggatcgcg
     5281 ttgtctccgc acccgctgca ccagcgcggt acgccgccct ggtagtcctc gagtgtgtgg
     5341 cgttcctcgt gcagcttgat gatgcactgg ctgagtgcgg agcccatcgt ttactttcct
     5401 tcgctcaatt tggcgcgaat ggcgttgacc accaggccgg gtttgatcgg ctggccacgg
     5461 gcttcggtcc agcagtcgac atcgaccagc gtgcgcgccc gcagcatcat ggcgagcggg
     5521 gcgtagcgcc ggttctcttc gtcgatcagt tcgtcttccg cgggacggtc ggcccaggtg
     5581 ccttcgaccg tcatcacctt ccggaagcgc ttcaggatgg tcgcaatgcc ggagggcatc
     5641 ggctggatga agcgcatctg cagcgccgac accttcagcc cctcgtcgcg cacgcgctgc
     5701 acggcctcct cgatgatgcc ctgcgtactg ccccagccga cgaccatcag gtcaccctcc
     5761 gggtcgccat acacggacgg cgtcttcagc gtcttctgca gcgcggccag cttcaggctg
     5821 cgggcgcgca gaccttcctc gttgatcgcc gggtcgtagg cgaccttgct cgcgcgatcg
     5881 tgggccagcc ccgtcaccgt gtgcattcca ttgggttgac cgggtgagaa gcggcgcgag
     5941 atcccggtca tcttgtccca gtcgtagggc ttcgcacccg ggggcagcgg cgactggtcg
     6001 atcggaggcg cgagccagtc ctcggagaag tccgggcgtg cgaacggctg ctgcgccgtg
     6061 gccaggctcg cgtcggtcag gaccaccacg accatattga aggtctcggc gatcttgcgt
     6121 gccgtgatca tcgagtagaa gcagtcctcg atgccggtcg ctgcgatcac caccttcggc
     6181 gcgtcgccgt ggctgccgta aatggcggtc agcaggtcgg cctgttcgac cttggttggc
     6241 tgcccggtgc tgggtccacc gcgctgcacg ttgacgacaa ccagcgggat ctcggccatc
     6301 acggcgagtc cgatgccttc ctgcttcagc gagtagcccg ggcccgaggt aattgtcact
     6361 gcacagcggc cggcatacga cgcgccgatc gcgaacgcac aggcggcgat ttcgtcctcg
     6421 gcctggtgca cgatgccgcc gacccgctcg aagcactccg acaggtagtg cgacgc
//
LOCUS       DATIRT010000092         1926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1584464, whole genome shotgun sequence.
ACCESSION   DATIRT010000092 DATIRT010000000
VERSION     DATIRT010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1584464"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VLN25_02300"
     CDS             complement(<1..613)
                     /locus_tag="VLN25_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSQ09436.1"
                     /translation="MNLSRLLRQSLRMTVRDWRAGELRLLAIALVIAVAAVTSVGFFV
                     DRIRLGLERDAAQLLAADLVLSSDQPITEALRTRARGAGLQTADTVTFPSMALNSGDP
                     DRNALAAVKAVSDGYPMRGTLRLSDEPGGAETVARAIPPAGTAWVDAQLLQVIGARPG
                     ENLQLGEKFFRIDRVIVIEPDRGTGFINFAPRVMINLADLQSTE"
     gene            762..>1926
                     /locus_tag="VLN25_02305"
     CDS             762..>1926
                     /locus_tag="VLN25_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09437.1"
                     /translation="MSTLSLLAKSAVAAAVAGASVTGTTNSGPPPTAGTALTIYSHAA
                     PGAVSPELYRSPGRGAVPGYAVIRQERDLDLNKGRNAVRFTDVAALIDPTTVVFESLT
                     DGKGTTVLEQNYQFDLVSADKLLQKYIDRPIAVEQVRGQATETFNGTLLSTQGGLVLR
                     RDDGTVQMVPHHAGVKLPALPGGLITRPTLVWDVAAQRAGSHRTRVSYQTQGMTWWSD
                     YNVTYAEGSNANACRMDIGAWVSIINQSGAGYADARLKLVAGDVHRATASGTAQFPAS
                     LGKALRSEEKSNGFVEKSFFEYHLYTLGRTTTLPDYSTKQIELFPAARRVPCEKLLVY
                     YGAPSGHRGFLSSPATDRNYGVHSNRKVDVYLRFANAEASGMGMPLPAGRVRVS"
BASE COUNT          337 a          654 c          627 g          308 t
ORIGIN      
        1 gctccgttga ctgcaggtcg gccaggttga tcatcacgcg cggcgcgaag ttgatgaagc
       61 ccgtcccgcg atccggttcg atgacaatta ctcgatcgat gcggaagaac ttctcgccaa
      121 gctggaggtt ttcacccgga cgggcaccga tcacctgcag caactgcgcg tcgacccacg
      181 ctgtgcccgc cggagggatc gcgcgcgcca ctgtctccgc gccaccgggt tcatcgctca
      241 gccgcagtgt gccgcgcatg ggatagccgt ccgaaacggc cttcacggct gcgagcgcat
      301 tgcggtcggg gtcgcccgaa ttcagggcca tgctcgggaa cgtgacggta tccgcggttt
      361 gcaggcccgc gccacgtgcc cgtgtgcgca gcgcttcggt gatcggctga tccgagctca
      421 gcacaaggtc cgcggcgagc aactgggccg cgtcgcgctc caggccgagg cggatgcgat
      481 cgacaaagaa ccccacgctc gtcaccgcgg ccaccgcgat gaccagtgca atggcgagca
      541 ggcgcagctc gccggcgcgc cagtcgcgga cggtcatgcg cagggattgg cgaagcaggc
      601 gggacaggtt catcggacag gtgactctat tagagcaaga ctcatggcgg cggggagtat
      661 gacattgcgc ccgcagtgcc ctgcggccac gccgcaccgt gttaacccct ttcgctagcc
      721 ccgcgcgttg acactcaggt ccatcacgcc aggagacccc cgtgtcgact ctcagccttc
      781 ttgccaagtc cgccgtggcc gccgccgtgg ccggcgccag cgtcacggga accaccaatt
      841 cgggcccgcc tccaacggcg ggaactgcgc tgaccatcta cagccacgcc gccccgggcg
      901 ccgtgtcgcc cgagctgtat cgcagcccgg gccgaggcgc tgttcccggc tacgccgtca
      961 tacgccagga gcgcgacctc gacttgaaca agggacgcaa tgccgtgcgc ttcacggacg
     1021 tggctgcact gattgaccca accaccgtgg tgttcgaatc gctcaccgac ggcaagggca
     1081 ccacggtgct tgagcagaac taccagttcg atctcgtcag cgccgacaag ctgctgcaga
     1141 agtacatcga ccggccgatc gccgtggaac aggttcgcgg gcaggcgacc gaaacattca
     1201 atggcacgtt gctgtcgacg cagggcggcc tcgtgctgcg acgtgacgac gggaccgtgc
     1261 agatggttcc gcaccatgcc ggcgtgaagc tcccggccct tcccggcgga ctcatcacgc
     1321 ggcccacgct cgtgtgggac gtcgcggcac agcgcgccgg gagtcaccgc acgcgtgtgt
     1381 cgtatcagac gcagggcatg acctggtggt cggactacaa cgtcacctac gcggaagggt
     1441 cgaacgcgaa cgcctgtcgc atggacatcg gcgcgtgggt cagcatcatc aaccagtcgg
     1501 gagcgggcta tgcggatgcc cggctcaagc ttgtcgcggg cgatgtgcat cgcgctactg
     1561 catccggtac ggcccagttt ccggcgtcgc tcggcaaggc actgagaagc gaggaaaagt
     1621 ccaacggctt cgtcgagaag tcgttcttcg agtaccacct ttacacgttg ggccgcacca
     1681 ccaccctgcc cgactactcg accaagcaga tcgaactgtt tccggcggcg cgtcgcgtgc
     1741 cgtgcgagaa gctgctggtc tactacggcg cgccgagtgg gcaccgcggc ttcctgtcgt
     1801 cgccggccac cgatcgcaac tacggcgtgc acagcaaccg caaggtcgat gtctacctgc
     1861 ggttcgccaa cgccgaggcg agcggcatgg gcatgccgct gccggcgggg cgcgtgcgcg
     1921 tgtcca
//
LOCUS       DATIRT010000093         5184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_896245, whole genome shotgun sequence.
ACCESSION   DATIRT010000093 DATIRT010000000
VERSION     DATIRT010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896245"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VLN25_02310"
     CDS             complement(<1..270)
                     /locus_tag="VLN25_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HSQ09438.1"
                     /translation="MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAITLVLSKKFGGEA
                     RAYDQIDAAPEEKARGITINTAHVEYETKNRHYAHVDCPGHADYVK"
     gene            complement(313..2415)
                     /gene="fusA"
                     /locus_tag="VLN25_02315"
     CDS             complement(313..2415)
                     /gene="fusA"
                     /locus_tag="VLN25_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HSQ09439.1"
                     /translation="MARKTPIERYRNIGISAHIDAGKTTTTERILFYTGVNHKLGEVH
                     EGAATMDWMEQEQERGITITSAATTTFWKGMDLSFPEHRINIIDTPGHVDFTIEVERS
                     MRVLDGACMVYCAVGGVQPQSETVWRQANKYGVPRLAFVNKMDRTGANFFKVFEQMRL
                     RLKANPVAIQIPIGSEDNFKGIVDLVKMKAIFWDEASKGMKFEYAEIPAELIDTAKEW
                     REKMVEAAAEANEELMTKYLEGGALTEIEVKQGLRHRTIAHEITPMLCGSAFKNKGVQ
                     AMLDAVVEYLPSPVDRPAVMGENDRGVSEERPANDEAPFAALAFKIMTDPYVGQLTFL
                     RVYSGVLKSGDTVINSIKSRKERVGRLLQMHANQRDEIKEVCAGDIAAAVGMKDVTTG
                     DTLCDPDKVITLERMIFPEPVISQAVEPKTKADQEKMGIALNRLAQEDPSFRVHTDEE
                     SGQTIISGMGELHLEILVDRMKREFSVDASVGKPQVAYRETIRKTVENAEGKFIKQSG
                     GRGQYGHVVLKLEPQVPGTGFAFVDAIKGGVVPREYIPAVEKGINETLKAGVLAGYPV
                     VDVKVTLHFGSYHEVDSNENAFKMAASMGFKDGMRAASPALLEPIMAVEVETPEEKMG
                     DVMGDLSSRRGVIQGMDDLPGGGKAIRAEVPLAEMFGYSTTLRSLTQGRATYTMEFKH
                     YAEAPKSVAEAVISAKTK"
     gene            complement(2477..2947)
                     /gene="rpsG"
                     /locus_tag="VLN25_02320"
     CDS             complement(2477..2947)
                     /gene="rpsG"
                     /locus_tag="VLN25_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HSQ09440.1"
                     /translation="MPRRREVPKREILPDPKFGSQDVTKFVNVIMLDGKKAVAERIVY
                     GALTQIETKAGKNALEVFSLALSNVKPMVEVKSRRVGGANYQVPVEVRPVRRMALAMR
                     WLREAAKKRSEKSMPLRLAGELIEAAEGRGGAMKKREEVHRMAEANKAFAHFRF"
     gene            complement(3077..3454)
                     /gene="rpsL"
                     /locus_tag="VLN25_02325"
     CDS             complement(3077..3454)
                     /gene="rpsL"
                     /locus_tag="VLN25_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HSQ09441.1"
                     /translation="MPTINQLVRKPRETTRIKSKSPALKNSPQKRGVCTRVYTTTPKK
                     PNSALRKVAKVRLVNGYEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHIVRGSL
                     DTQGVKDRKQSRSKYGAKRPKAA"
     gene            complement(3829..>5184)
                     /locus_tag="VLN25_02330"
     CDS             complement(3829..>5184)
                     /locus_tag="VLN25_02330"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113043.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. Subunit beta'
                     binds to sigma factor allowing it to bind to the -10
                     region of the promoter; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HSQ09442.1"
                     /translation="TVRFTATMRYVTNVKGEQVAISRSGEVTIVDDNGRERERHKVPY
                     GAMLLVADGKQVKAGTQLASWDPLTRPIITEYAGTVKFEHVEEGVTVARQIDEVTGLS
                     TLVVIDAKRRTASSKGVRPQVKLLNEAGAEVKIPGTEHSVTISFPIGALIVVRDAQQV
                     GVGEVLARIPTESQKTRDITGGLPRVAELFEARSPKDAGMLAEVTGTVTFGKETKGKQ
                     RLIITDLDGKEHEFLITKDKHLLVHDGQVVNRGESIVDGPADPHDILRLLGVEALARY
                     IVDEVQDVYRLQGVKINDKHIEVIVRQMLRRVQISDPGDAPFITAEQVERSELFDEND
                     KAIAEGKRPALYENILLGITKASLSTDSFISAASFQETTRVLTEAAIMGKKDDLRGLK
                     ENVIVGRLIPAGTGMAFHRARKEKEAADALEQQQLAAMEALAAEGESEPGADESTEVT
                     E"
BASE COUNT          848 a         1714 c         1547 g         1075 t
ORIGIN      
        1 cttgacgtag tcggcgtgac ccgggcagtc cacatgggcg tagtgccggt tcttggtctc
       61 gtactcgacg tgggcggtgt tgatggtgat gccgcgcgcc ttttcctccg gcgccgcatc
      121 aatctggtca tacgctcgcg cttcaccgcc aaacttcttc gacaacacca gcgtgatcgc
      181 cgccgtcagg gtggtcttgc catgatccac gtgaccaatc gtgcccacat tcacatgcgg
      241 cttcgtacgc tcaaactttc cttttgccat cgctaggtcc tcaaagaacg gttgttcgtg
      301 ttgtgacgac tcttatttcg tcttcgcgga gatcacagcc tcggcgaccg acttcggcgc
      361 ttcggcgtag tgcttgaact ccatcgtgta ggtcgcgcga ccctgcgtaa gcgaacgcag
      421 tgtcgtcgag tatccgaaca tctcggccag cggcacctcc gcgcggatgg ccttgccgcc
      481 gcccggcaga tcgtccatgc cctggatgac gccgcgacgc gacgacagat cgcccatgac
      541 gtcgcccatc ttctcttccg gcgtctcgac ctcgaccgcc atgatcggtt cgagcagtgc
      601 gggagacgct gcgcgcatgc cgtccttgaa tcccatcgag gccgccatct tgaaggcgtt
      661 ctcgttcgaa tcgacctcgt ggtacgaacc gaagtgcagc gtgaccttga catcgacgac
      721 cggataaccg gccagcacgc cggccttcag cgtttcgttg atgcccttct cgaccgccgg
      781 gatgtactcg cgcggaacca cgccgccctt gatcgcatcg acgaaggcaa agcccgtccc
      841 gggtacctgg ggctccagct tcagcacgac gtgaccatac tggccgcgtc ctcccgactg
      901 cttgatgaac ttgccttcgg cgttctcgac cgtcttgcgg atcgtctccc gataggccac
      961 ctgcggcttg ccgaccgagg cgtcgacgct gaactcgcgc ttcatgcggt cgacaaggat
     1021 ctcaaggtgc agctcgccca tgccagagat gatggtctga cccgactctt catccgtgtg
     1081 cacgcggaac gacgggtctt cctgcgccag gcgattgagc gcaatgccca ttttttcctg
     1141 atcggccttg gtcttcggct ccacggcctg ggaaatcacc ggctccggga agatcatgcg
     1201 ctcgagcgtg atgaccttgt ccgggtcgca cagcgtgtcg ccggtggtca cgtccttcat
     1261 gccaaccgcc gccgcgatgt cgcccgcgca cacttccttg atctcgtcgc gctggttggc
     1321 gtgcatctgc agcaggcgtc cgacgcgctc cttgcgactc ttgatcgaat tgatgaccgt
     1381 gtcgccggac ttcagcacgc cggaatacac gcgcaggaag gtgagctggc cgacgtaggg
     1441 gtcggtcatg atcttgaacg ccagcgcggc gaatggcgcc tcgtcattgg ccggccgctc
     1501 ttcggatacg ccccggtcgt tctcgcccat gaccgccgga cgatcgaccg ggctcggcaa
     1561 gtactcgacg accgcatcga gcatcgcctg cacacccttg ttcttgaagg ccgagccgca
     1621 aagcatcggc gtgatctcgt gcgcaatcgt gcgatggcgc aggccctgct tgacttcgat
     1681 ctccgtcagc gcgccacctt cgaggtactt ggtcatcagt tcctcgttgg cctcggctgc
     1741 cgcctcgacc atcttctcgc gccactcctt ggccgtgtcg atgagctcag cggggatctc
     1801 ggcatactcg aacttcatgc ccttgctggc ctcgtcccag aagatcgcct tcatcttgac
     1861 gaggtcgacg atgcccttga agttgtcctc ggaaccgatc ggaatctgga tcgcgacggg
     1921 attggccttc aggcgcagac gcatctgctc gaagaccttg aagaagttgg cacccgtgcg
     1981 gtccatcttg ttgacgaaag cgagccgagg cacaccgtac ttgttggcct gacgccagac
     2041 ggtttccgac tgcggctgga caccgcccac cgcgcaatag accatgcacg ctccgtcgag
     2101 cacgcgcatg ctgcgctcga cctcgatcgt gaagtcgacg tggcccgggg tatcgatgat
     2161 gttgatgcgg tgctcaggga acgaaaggtc catccccttc cagaacgtgg tcgtcgccgc
     2221 ggacgtgatc gtgatgccac gctcctgctc ctgctccatc cagtccatcg tcgcggcgcc
     2281 ttcatgcacc tcgccgagct tgtggtttac accggtgtaa aacaggatgc gctcggtcgt
     2341 cgtagtcttg ccggcgtcga tatgcgccga gatgccgatg ttccgatacc gctcgatggg
     2401 ggtcttgcgt gccataaggc tcacctagta aacctcgctg cggaccgccc tgtggccggt
     2461 ccgcacagaa aatttgttag aagcggaaat gcgcgaacgc cttgttcgcc tccgccatgc
     2521 gatgcacttc ctcgcgcttc ttcatcgcgc cgccacgacc ctcggctgct tcaatcagct
     2581 cgccggccag gcgcagcggc atcgacttct cgctgcgttt cttggccgcc tcgcgcagcc
     2641 agcgcatcgc aagtgccatc cggcgcacgg gccgaacttc gaccggaacc tgataattcg
     2701 cgccgccgac acgccggctc ttgacttcga ccatcggctt cacgttcgac agggccagtg
     2761 aaaacacttc gagcgcgttc ttgccggcct tggtctcgat ctgggtcaag gcgccataga
     2821 cgatgcgctc agccacggcc ttcttgccgt cgagcatgat cacgttgacg aacttggtga
     2881 cgtcctggga tccgaacttg ggatccggaa ggatttcacg cttaggtact tcacgacgac
     2941 ggggcattgc tcacctcatt tgcctggact tggcttcagt cgttgggcgg aacacgctgg
     3001 ctccgcaacg gcggctccat cgaaacgagc cgcgacttac tcggccgcct gcatgtgcag
     3061 gcagccggtt tgaaaatcag gctgccttcg gacgcttggc gccgtatttc gagcgggact
     3121 gcttgcggtc cttcacgccc tgcgtgtcga gcgagccgcg cacgatgtgg tagcgcacgc
     3181 cgggcaggtc cttgacgcgg ccgccgcgaa tcaagaccac cgagtgttcc tgcaggttgt
     3241 ggccctcgcc gccgatgtac gagatcacct cgtagccgtt gaccaggcgc accttggcca
     3301 ccttgcgcag cgctgagttc ggcttctttg gcgtcgtcgt gtagacgcgc gtgcatacgc
     3361 cgcgcttctg cgggctgttc ttgagcgccg ggctcttgct cttgatgcgg gtcgtttcgc
     3421 gcggcttgcg tacgagctga ttgatggttg gcatgcgacg tccgttatcg aaaatccaat
     3481 gggcacgccg cccaaagcga cgtgcgaaaa ctcggtgcaa ctactcagtg caacttttca
     3541 gtgcaaccca aaagaccacc agggactgca ctgcttcggc ggcgcgttcg ggaacccctg
     3601 cgccttggac tcgaccctta cgctgacagg ccgggagttc cgaggactcg gaaaccacgg
     3661 acaccaggcg agggcgggca aagaacgcaa cgcccgggcg ggcgccgaaa agctcgccat
     3721 tatcgctagc cgggccacgc ggtgtcaagg cgactgccgg gcggccgcca gggaggcccc
     3781 agaaaaacgg cccgcactgg cgggccgttg tccaagggtc gcttgctgct attccgtgac
     3841 ttcggtcgac tcgtccgcgc ccggctcgct ctcgccttcg gcggccagtg cctccatggc
     3901 cgccaattgc tgttgctcca gcgcgtcggc cgcttccttt tccttgcggg cgcggtggaa
     3961 cgccatgcca gtacccgccg gaatcaggcg gccgacgatc acgttctcct tgaggccgcg
     4021 cagatcgtcc ttcttgccca tgatcgcggc ctcggtgagg acgcgggtgg tctcctggaa
     4081 cgatgctgcg gaaatgaacg agtcggtcga caaagaggcc ttggtgatgc cgagcaggat
     4141 gttctcgtac aaggcgggcc gcttgccttc cgcgatcgcc ttgtcgttct cgtcaaagag
     4201 ttcagaacgt tcgacctgtt cagcggtgat gaacggcgcg tcacccggat ccgagatctg
     4261 aacccggcgc agcatctgcc ggacgatgac ctcgatgtgc ttgtcgttga tcttcacccc
     4321 ctgcaggcgg tagacgtcct ggacctcgtc gacgatgtag cgcgcaagcg cctcgacacc
     4381 gagtagacgc aggatgtcgt gcggatccgc cggtccatcg acgatggact cgccgcgatt
     4441 cacgacctgc ccgtcgtgca ccagcagatg cttgtccttg gtgatcagga actcgtgctc
     4501 cttgccatca aggtcggtga tgatgagccg ctgtttgcct ttggtctcct tgccgaacgt
     4561 gaccgtccct gtaacttcgg ccagcatgcc cgcatccttc ggcgagcgcg cctcgaagag
     4621 ctcggccaca cgcggcagac ccccggtgat gtctcgggtc ttctgcgatt cggtcgggat
     4681 ccgcgccagc acctcgccga caccgacctg ctgggcatcg cgcacgacga tgagcgcacc
     4741 gatcgggaag ctgatcgtca ccgagtgctc ggtgccgggg atcttcactt ctgccccggc
     4801 ttcgttcagc aacttgacct gcgggcgcac ccccttgctc gacgccgtcc ggcgcttggc
     4861 gtcgatcact accagcgtcg acaggccagt gacctcgtcg atctgcctgg cgacggtcac
     4921 gccttcctcg acgtgttcga acttaacggt accggcgtac tcggtgatga tcggtcgggt
     4981 cagcgggtcc cagctggcga gctgcgtgcc ggccttgacc tgcttgccgt cggccaccag
     5041 cagcatggcg ccatagggca ccttgtggcg ctcacgctcc cggccattgt cgtcaacgat
     5101 ggtgacttcg cccgatcgcg agatggcgac ctgctcaccc ttgacgttcg tgacatagcg
     5161 catcgtcgcg gtaaagcgca ccgt
//
LOCUS       DATIRT010000094         3036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1683119, whole genome shotgun sequence.
ACCESSION   DATIRT010000094 DATIRT010000000
VERSION     DATIRT010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1683119"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VLN25_02335"
     CDS             <1..213
                     /locus_tag="VLN25_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HSQ09443.1"
                     /translation="DIAEKAGCNKCHATDAKKMGPSYKDIAKKFKGNAAAEADIVKKL
                     KTGTGHPKASASDADLTAVTKWILVM"
     gene            566..1786
                     /gene="purT"
                     /locus_tag="VLN25_02340"
     CDS             566..1786
                     /gene="purT"
                     /locus_tag="VLN25_02340"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HSQ09444.1"
                     /translation="MSPTSNWPHPLGTPCSHAATRVLLLGAGELGKEVLIALQRLGVE
                     TIAVDRYAGAPGHQVAHRAHVVDMTNAAELLALIESERPHIVVPEIEAIATAALGEVE
                     KSGLARVVPTARAVQLTMNREGIRRLAAETLQLPTSPYRFASTFEETTAAAREVGFPC
                     IIKPVMSSSGKGQSRVNAADELPAAWEYANAGGRVALGRVIVEGFIDFDYEITLLTVR
                     ALGRDGRIETAFCEPIGHLQVAGDYVESWQPHPMSPVALATARDYAQRITDDLGGTGI
                     FGVELFVKADRVWFSEVSPRPHDTGMVTMATQGQNEFELHARAILGLPIDASLRNPGA
                     SAVIYGGHETKALVFDGVDDALSLPGVDVRLFGKPESFIKRRMGVALARAGTVDEARA
                     TAKAAAGRIRPRPA"
     gene            1906..2559
                     /locus_tag="VLN25_02345"
     CDS             1906..2559
                     /locus_tag="VLN25_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSQ09445.1"
                     /translation="MKILVVDDHSLITDALSVLFLDLDPEAEVITTRTAETALQLQQE
                     HPDLDLVVLDLGLPGVSGTSLLEAMVAQAPEMKILVLSGQQDQRNVMRVLQLGAAGFV
                     PKSMASETLLSAVKFVLSGGVYIPADLLEEVNRSGMFAAPDRPREAGASRVELTERQE
                     QVLQLLARGAPIKIICRELGLSEGTVKTHVTAIYRAFGASNRTEALLAARRHGYDIG"
     gene            complement(2577..>3036)
                     /locus_tag="VLN25_02350"
     CDS             complement(2577..>3036)
                     /locus_tag="VLN25_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09446.1"
                     /translation="RLLRSWGAEVVPSTGYNAQLIKRISLHERIDAIIADHNLGPHSI
                     SGVEAVFKIRELVGMPLPTIMLTAVQRADVLAEFQRVMGEHLLASPDSSAIARSPHEE
                     PIVLQKPTTAAVLNNSLGELLGLTTLPMVRTVLPPPPAIADTVPPKAAAG"
BASE COUNT          531 a         1023 c         1007 g          475 t
ORIGIN      
        1 gacatcgccg aaaaggccgg ctgcaacaag tgccacgcca ccgatgccaa gaaaatggga
       61 ccgtcctaca aggacatcgc caagaagttc aagggcaacg cggccgctga ggccgacatc
      121 gtcaagaagc tgaagactgg caccggccac ccgaaggcca gcgcctccga cgcggacctg
      181 accgccgtca ccaagtggat cctcgtgatg tagttcggcg cgagcccaca agaaaaagcc
      241 ggctgacgcc ggcttttttg tccgctgtcc ggacctgcga cttggcggac ggcccggatg
      301 aggagggctg gcgcccccca gtccggatct tgttcttgtc gattggcgaa gcgcggcgga
      361 ttgggattcg cagccgctgc tgagccactg ctagtgtggt cgttgcccct gctctgcact
      421 tgcgcgagat caagcgcgtc cgtcccgcag gtcggccgca tcgccgccgc tgctcatggg
      481 acagcgggga cagtgcccgc ccggcaggag tgcacgccac gtcggcgtgc gatagtcccg
      541 aacacacacc cgccacgatg cactcatgag ccccacctcg aactggccac accctctcgg
      601 aacgccctgc tcacatgccg caacgcgtgt actgctgctc ggggccggcg aactcggcaa
      661 ggaagtcctg atcgctctgc aacgactggg ggtggagacg attgccgtgg accgttatgc
      721 cggtgcaccc ggtcaccagg tggcgcaccg ggcgcatgtc gtcgacatga cgaacgccgc
      781 tgaactgctg gcactgattg aatcagaacg gccccacatc gtcgtgccgg agatcgaggc
      841 gatcgccacg gcggcgctgg gcgaagtcga gaagtccggc ctggcgcggg tcgtacccac
      901 cgcacgcgcc gtacaactga cgatgaaccg ggaaggcatc cgacgcctgg cggcggaaac
      961 cttgcagctg ccgacgtcac cctaccgctt tgccagcacc ttcgaagaaa cgaccgcggc
     1021 cgcacgggag gtcggatttc cctgcatcat caagccggtc atgtcgtcgt ccggcaaagg
     1081 ccagagccgg gtcaacgcgg cggacgaact tccggcggca tgggagtacg ccaacgcggg
     1141 cggccgggtc gcgctcggtc gcgtgatcgt cgagggcttc atcgacttcg actacgagat
     1201 cacactgctg accgtccgcg cactggggcg cgacggacgc atcgagactg cgttctgtga
     1261 accgatcggc cacctgcagg tcgcgggcga ctatgtcgag agctggcagc cgcatccaat
     1321 gagcccggtc gcactggcca ccgcgcgcga ctatgcgcag cggatcaccg acgatctggg
     1381 cggaaccggc atcttcggcg tcgagctctt cgtcaaggca gatcgggtct ggttctccga
     1441 ggtcagcccg cggccgcacg acaccggcat ggtgacaatg gccacgcagg ggcagaacga
     1501 attcgagctg cacgcgcgcg ccatcctggg cctgccgatc gatgcaagcc tgcgcaatcc
     1561 cggggccagt gccgtcatct acggagggca cgagaccaag gcgctcgtgt tcgatggcgt
     1621 tgacgacgcg ctatcgctgc ccggcgtcga cgtgcgcctg ttcgggaagc ccgagtcctt
     1681 catcaagcgc cgcatgggcg ttgcgctggc ccgcgccggc acggtcgacg aagcgcgcgc
     1741 gaccgccaag gccgcggcgg gacgcatccg gccacgaccg gcctgagccg atcgcccgcg
     1801 ggccaccgcg aaagcgccgc ttttccgggc agcgggccca tgagggtcgc ggtctacaat
     1861 cgcgccattc ggcagtgccg cgcctgagcc tccagaagct cgcccatgaa gatcctcgtt
     1921 gtagacgacc attccctgat tacggacgcg ctgtcggtgc tgttcctgga cctcgacccc
     1981 gaggccgagg tcatcacgac gcgcacggcc gaaaccgccc tgcagctgca gcaggaacac
     2041 ccggacctcg atctggtggt attggatctg ggacttccgg gcgtgtccgg tacgtcgttg
     2101 ctcgaagcga tggtcgcgca ggcgcccgag atgaagatcc tggtgctgtc gggccagcag
     2161 gaccagcgca acgtgatgcg cgtgctgcag ctcggtgccg ccggcttcgt gccaaagtcg
     2221 atggcctccg agaccctgtt atcggcggtc aagttcgtgc tgtcgggcgg ggtctacata
     2281 ccggccgacc tgctcgagga agtgaaccgc tcgggcatgt tcgccgcacc ggatcgtccg
     2341 cgcgaggcgg gcgccagccg cgtggaactg acggaacggc aggagcaggt actgcagttg
     2401 ctggcccgcg gcgcacccat caagataatc tgccgcgagc tcggtctctc ggaaggcacg
     2461 gtcaagacgc acgtcaccgc catctaccgc gcgttcggtg cgtccaaccg caccgaagcg
     2521 ctgctggccg cgcgccggca cggctacgac atcggctagc gccgtcgtgc cgggtcctac
     2581 ccggcggccg ccttgggcgg caccgtatcg gcaatcgcgg gcggaggcgg cagcacggtc
     2641 cgcaccatcg gcagcgtcgt caatcccagc agttcaccga ggctattgtt cagtacggcg
     2701 gccgtcgtcg gcttctgcag cacgatgggc tcctcgtgcg gactgcgcgc gatggccgac
     2761 gagtccggac tggccagcaa gtgttcgccc atcacgcgct ggaactcggc caacacgtcg
     2821 gcacgctgca ccgccgtcag catgatagtc ggcagcggca tgccgacaag ttcgcggatc
     2881 ttgaataccg cctcaacgcc gctgatcgaa tgcggtccga ggttgtggtc ggcgatgatc
     2941 gcgtcgatgc gctcgtgcag gctgatgcgc ttgatcagct gcgcgttgta gccggtcgag
     3001 ggcacgacct cggcgcccca cgagcgcagc agtcgc
//
LOCUS       DATIRT010000095         4243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1093307, whole genome shotgun sequence.
ACCESSION   DATIRT010000095 DATIRT010000000
VERSION     DATIRT010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093307"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1301)
                     /gene="ccmI"
                     /locus_tag="VLN25_02355"
     CDS             complement(<1..1301)
                     /gene="ccmI"
                     /locus_tag="VLN25_02355"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HSQ09447.1"
                     /translation="MTAFIAIATVMVAIALAWLLWPLLRAARRPVVERHVANASIYGD
                     QFADLDADLRRGTISEASYAEARGELERRLLDEGRAQGGNSTAPVSSNRATAIVVALA
                     VPLVAGLMYWKLGAPDAFSPMATAPTAPDASHQMTPEQLDGMVQQLAARLEKEPGNVE
                     GWVILARTYYAMRKFPEAAAAYEKLTQLVPDEPDLLADYADALAMSQGRDLSGRPFAL
                     VQAALKINPTHWKALAMAGTAAFDRKDYKGAVDYWERLRSSQPADSPIAQSIAVSISE
                     ARKLGGLPPAPAIAAAPQKASPATMPSPAAAAPASEASKGTTAAQGTTVGGIVNLSDA
                     IKAKAAPTDTVFIFARPAEGSRMPLALTQVKVSELPARFTLDDTMAMSQDMKLSTTPS
                     VIVGARVSKSGRPMPSSGDLEGLSKPVAIGTKDVAVTIDRVL"
     gene            complement(1298..1771)
                     /locus_tag="VLN25_02360"
     CDS             complement(1298..1771)
                     /locus_tag="VLN25_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HSQ09448.1"
                     /translation="MRRLIAAVLVGCVPLLAQASTEPTMLDPVAQRRLVDLSAQLRCL
                     VCQNQSIAESNAELAVDLRNQINEQIKAGRSDKEIVDFMTTRYGDFVLYRPPFKAATA
                     LLWIGPIALLLLAVFFFYRTLVSRRVRVEDHPLTEAERAEAQRLLATAENNKNSS"
     gene            complement(1768..2304)
                     /locus_tag="VLN25_02365"
     CDS             complement(1768..2304)
                     /locus_tag="VLN25_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HSQ09449.1"
                     /translation="MRKVLVYLLPLAGFLVLAVFLLRGLWLNPREVPSPFIDKPAPMF
                     SLIQLARPEAKFSPADMKGQVWLLNVWASWCVACRQEHPLLVQLAQKKAVPIVGLNYK
                     DKPDAAKAWLAQWGDPYDLSVRDLDGRIGIDYGVYGVPETFVIDKNGVIRFKQIGPIT
                     EEVWQKKMLPLLEELRKA"
     gene            complement(2311..4242)
                     /locus_tag="VLN25_02370"
     CDS             complement(2311..4242)
                     /locus_tag="VLN25_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HSQ09450.1"
                     /translation="MLIATVQAVLPIAGAARGDRVWMSVARPAARGQAFFVTLAYLCL
                     LASFINNDFSVINVATNSNSQLPIQYRIAASWGSHEGSLLLWVLMLAWWTLAVATFSK
                     HMPDDMVARVIGVQGFVSVGFLLFMLTTSDPFDRLFPPAPDGRDLNPLLQDPGMVFHP
                     PMLYMGYVGFSVAFAFAIAALLGGKLDATWARWSRPWTTLAWGFLTIGIGLGSWWAYY
                     ELGWGGWWFWDPVENASFMPWLVGTALIHSLAVTEKRGSFKSWTVLLAITTVSLSLLG
                     TFLVRSGVLTSVHAFATDPRRGVFILGFLVIVIGASLALFAWRAPKVGLGGKFDTVSR
                     ESFLLMNNVLLAVACASVLLGTLYPLFLDALNLGKISVGPPYFDAVFVPIMAPLVFLM
                     GVGPLARWKKAEVPDLARRLKWALGVSFATAVIVPFIAGRWTPMIAFGLFLAFWVIAS
                     AAVGIRERVRHIRGGLWARLAAQGRAYWGMQLAHLGIAVFILGVTLVKGYEVERDVRM
                     DVGDSVTVGSYAFKFEGTREIAGPNYRGARGTVEVTKNGKFVEHLYPEKRMYNVQQMP
                     MTEAAIDSGVLGDLYVSLGEPVANGAWSVRIYSKPFITWIWGGCVLMAIGGLVALSDR
                     RYRFFARRSTVVEGAQARV"
BASE COUNT          819 a         1408 c         1329 g          687 t
ORIGIN      
        1 aggacgcgat cgatggtgac tgccacgtcc ttcgttccga tcgcgacggg ctttgacagc
       61 ccctcgaggt cgccactgga gggcatcggt cggcctgact tcgacactcg tgctccgact
      121 atcacggagg gcgtcgtcga cagcttcatg tcctgcgaca tcgccatcgt gtcgtcgagc
      181 gtgaacctgg ctggcagctc cgataccttc acctgcgtca gtgccagcgg catgcgcgag
      241 ccttcggcag gacgcgcaaa gatgaacacg gtatccgtcg gtgctgcctt cgccttgatg
      301 gcgtccgaca ggttcacgat gccgcccacg gtggttcctt gcgccgcagt ggtgcccttg
      361 cttgcctcgc tggccggggc ggcagccgcc ggcgacggca ttgttgccgg tgacgccttc
      421 tgcggtgcag cggcgatcgc cggtgcaggc ggaagtccac cgagcttgcg ggcctcgctg
      481 atgctgacag caatggactg tgcgatcggg gagtctgcag gctgcgaaga acgcagtcgc
      541 tcccaatagt cgaccgcgcc cttgtagtcc ttgcgatcga acgcggcggt gccggccatc
      601 gccagcgcct tccagtgggt cggattgatc ttgagggccg cctgcacaag cgcgaacggg
      661 cgtccgctga ggtcacgtcc ctggctcatc gccagggcat ccgcatagtc agccagcaga
      721 tcgggttcgt cggggacaag ctgagtcagc ttctcgtagg cagcggcggc ctccggaaac
      781 ttgcgcatcg cgtagtacgt gcgcgcgagg atcacccagc cctcgacatt gccgggctcc
      841 ttttcgagcc gggccgccag ctgctgcacc atcccatcga gctgttcggg ggtcatttga
      901 tgcgaagcgt ccggcgctgt cggcgccgtg gccattggcg agaacgcgtc cggtgcaccg
      961 agcttccagt acatcagccc cgcgaccagc ggcaccgcca gcgcaaccac gatcgccgtg
     1021 gcccggttcg agctgactgg ggccgtgctg tttccgccct gcgcccgccc ttcgtccagc
     1081 agacgccgct cgagttcgcc gcgagcttcg gcgtaactcg cctcggagat ggtgcctcgc
     1141 cgaagatcgg cgtcgagatc ggcgaactga tcgccataga tcgaggcatt ggcgacatgg
     1201 cgctcgacca cggggcgacg tgcagcccta agcaacggcc acagtagcca agcgagggca
     1261 atggccacca tgacggtggc gatagcgatg aaggcagtca cgatgagttc ttgttgttct
     1321 cggcggtggc gagcaggcgt tgtgcttcgg cccgttccgc ctcggtgagt ggatggtctt
     1381 cgacacgcac gcggcggctg accagggtgc gatagaagaa gaagacggcg agcaacagca
     1441 gcgcgatcgg gccaatccag agcagtgccg tcgcagcctt gaagggcggt cggtacagaa
     1501 cgaagtcgcc gtagcgcgtc gtcatgaagt cgacgatctc cttgtcagag cgcccggcct
     1561 tgatctgctc gttgatctgg ttgcgcaggt ccaccgcaag ttcggcgttc gactcggcga
     1621 tggactggtt ctggcagacg agacagcgca gttgcgccga caggtccacc agacgcctct
     1681 gtgccaccgg atcgagcatt gtcggctcgg tcgacgcctg cgccagcaac ggcacgcagc
     1741 ctacgagtac ggcggcaata agccttctca tgccttcctc agttcctcga gcagcggcag
     1801 catcttcttc tgccagacct cttcggtgat cgggccgatc tgcttgaagc gtatcacgcc
     1861 gttcttgtcg atgacgaagg tctccggcac gccgtagacg ccatagtcga tgccgatgcg
     1921 accgtcgagg tccctgaccg acagatcgta cggatcaccc cactgcgcga gccacgcctt
     1981 ggcggcgtcg ggcttgtctt tgtaattcag gccaacgatc ggaacggcct tcttctgtgc
     2041 gagttgcacc agcagcgggt gttcctggcg gcaggccacg caccacgatg cccacacatt
     2101 cagcaaccac acctgtccct tcatgtcggc cggagaaaac ttcgcctccg gccgcgctag
     2161 ctggatcagc gagaacatcg gcgccggctt gtcgatgaac ggcgacggca cctcgcgcgg
     2221 attcagccac aacccgcgca gcaggaagac cgccaggacc aggaagcccg ccagcggcag
     2281 cagatagacg aggaccttgc gcattaccgc ctacaccctc gcctgtgcgc cttcaaccac
     2341 cgtcgagcgt cgcgcgaaaa accgatagcg gcgatccgac aacgcaacga gcccgccgat
     2401 ggccatcagc acgcagccac cccagatcca cgtgatgaac ggcttggaat agatgcgcac
     2461 gctccatgcg ccattggcga ccggctcgcc gagcgagacg tagaggtcgc cgagcacgcc
     2521 ggagtcgatt gcagcctcgg tcatcggcat ctgctgcacg ttgtacatgc gcttttcagg
     2581 ataaaggtgc tcgacgaact tgccgttctt ggtcacttcg actgtgcctc gggcaccgcg
     2641 gtagtttgga cccgcgattt cgcgcgtgcc ctcgaactta aaggcgtagc tgccgaccgt
     2701 cacgctgtct cccacgtcca tgcgcacgtc gcgctcgact tcgtagccct tgacgagagt
     2761 gacccccaag atgaacaccg cgatgcccag gtgcgcaagc tgcatgcccc agtaagcgcg
     2821 accctgcgca gccaggcgcg cccagagtcc gccccgaatg tgccgcacgc gctccctgat
     2881 gcccacagcg gccgaagcga tgacccaaaa ggccaggaac agcccgaagg cgatcatcgg
     2941 cgtccagcgg ccggcgatga agggaacgat gacagctgtc gcgaagctga cgcccagcgc
     3001 ccatttgagc cgccgcgcca ggtcgggaac ttctgccttc ttccagcgcg cgagcggacc
     3061 tacgcccatc aggaacacga gcggcgccat gatcggcacg aacaccgcat cgaagtacgg
     3121 cgggccgacc gagatcttgc cgagattgag tgcgtcgagg aacaacggat acagcgtacc
     3181 gagcagcacc gacgcgcacg caacggcgag cagcacattg ttcatcagca aaaacgactc
     3241 gcgtgacaca gtgtcgaact tgccgcccag tcccactttc ggcgcgcgcc aggcaaacaa
     3301 tgccagcgat gcgccgatga cgatgacaag gaagccgaga atgaagacac cgcgcctggg
     3361 atcggtcgca aacgcgtgca ccgacgtcag cacgcccgag cggacgagga aggtgcccag
     3421 cagggacagt gagaccgtcg tgatcgcgag cagcaccgtc cagctcttga aactgccgcg
     3481 cttttcggtc actgcgaggg agtggatcaa cgccgtgccg accagccacg gcatgaacga
     3541 tgcgttttcc accgggtccc agaaccacca gccgccccac ccgagctcgt agtaggccca
     3601 ccagctgccc agcccgattc cgatggtcag gaagccccag gccagcgtcg tccacggtcg
     3661 cgaccagcgc gcccaggtcg catcaagctt gccgcccagc agcgccgcga tcgcgaaggc
     3721 aaaggcgacg gaaaagccaa cgtatcccat gtagagcatc ggcggatgga acaccatgcc
     3781 cgggtcctgc agcagcgggt tgagatcccg accatcgggc gcgggcggga agagccggtc
     3841 gaacggatcc gaagtcgtca gcatgaagag caggaatccc accgacacga agccctgaac
     3901 gccgatgacg cgcgcgacca tgtcatccgg catgtgcttc gagaacgtcg ccaccgcaag
     3961 cgtccaccat gccagcatca gcacccacag cagcaacgac ccttcgtgcg agccccagct
     4021 ggcggcgatt cggtactgga tcggcaactg cgaattcgag ttggtcgcga cgttgatgac
     4081 ggagaagtcg ttgttgatga acgacgcgag caggcacaag tacgccagcg tgacgaagaa
     4141 ggcctggccg cgcgccgctg gacgtgcgac cgacatccag acccggtcgc cgcgcgccgc
     4201 gccggcgatc ggcagcacgg cctgcactgt ggcaatcagc agt
//
LOCUS       DATIRT010000096         2837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_405130, whole genome shotgun sequence.
ACCESSION   DATIRT010000096 DATIRT010000000
VERSION     DATIRT010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405130"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VLN25_02375"
     CDS             <1..798
                     /locus_tag="VLN25_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HSQ09451.1"
                     /translation="LSGVFAQPGRTNDFRELNHRLYLVATDLDTGLSVAFGGTDLDHV
                     PISRAVQASAALPGLFPPVEIDGRYFVDGALRKTLHASIALDQGVDLMLCLNPLVPFD
                     STPHHRTRDREHQLDKLVEGGLPVVLAQTFRSIIHSRLGAGLERYKTAYPHTDILLFE
                     PNRADADMFFTNLFSYSSRRRLCEHAYQKTREELWMRRHELAPLLRRHGISIRTDVLR
                     DESLTLVKSLRRSRGIWRETASTAATRLLTHTLDDLERWLRYSSAAA"
     gene            883..1542
                     /locus_tag="VLN25_02380"
     CDS             883..1542
                     /locus_tag="VLN25_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSQ09452.1"
                     /translation="MERKPPRRTRERILELSLRLFNDFGEPNVNTTLIAEEMNISPGN
                     LYYHFKNKDDIINCIFQQFEREIDKLLALPTDRVPNVEDAWLFLHVLFELIWKYRFFY
                     RDLNNLLANNRTLELKFKQLLNQKVKVARGLCDGLAQAGELQAGAREIEALATNMVVV
                     ATYWLSYSYVLDPRRFNEPAVIGAALQRGCYQVMALTSPYLTGQSRALFDQLAAEYLN
                     D"
     gene            1557..2336
                     /locus_tag="VLN25_02385"
     CDS             1557..2336
                     /locus_tag="VLN25_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09453.1"
                     /translation="MAKAWAKFPYPDKKYVYTAATLKKAWDRLHKGDAEPFPKAEAVV
                     EAWIAFHAGEFQKASDLGLKAGAEGASVANKAESIYANYLEDSDKRKLELFEEVAGRA
                     EALQAKEPRNANAFYWQAYSLGRYAQGISVGKALAQGIGGKVKVALETTIKLAPKHGD
                     AHIALGSYHAEVIDKVGAMIGGLTYGAKKDVALKNYQAALKLMPDSAIARIEYANGLV
                     MLEGKKALKQAEELYEAAAACEPMDAMERLDVELAKSELED"
     gene            complement(2387..2536)
                     /locus_tag="VLN25_02390"
     CDS             complement(2387..2536)
                     /locus_tag="VLN25_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09454.1"
                     /translation="MTLCPIAIVAGCEKCPAFKICPLKGVVGNPPKPEEPHKASARKK
                     SSHGK"
     gene            complement(2614..>2837)
                     /locus_tag="VLN25_02395"
     CDS             complement(2614..>2837)
                     /locus_tag="VLN25_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HSQ09455.1"
                     /translation="WQWIRSPKGVLDDGRKVTADLVRRLIPEELAKVKGAVGPDSPTY
                     DRAAVIFEKMSTQEGFAEFLTLPLYEEIA"
BASE COUNT          542 a          937 c          875 g          483 t
ORIGIN      
        1 ctgtcaggag tcttcgctca gccgggccgg accaatgact ttcgtgagct gaaccaccgc
       61 ctctacctgg ttgcgaccga tctcgacacc ggcctgtcgg ttgcattcgg aggaacggac
      121 ctggatcacg ttcctatctc gcgcgccgtg caggcgtccg ccgcgctgcc cggcttattc
      181 ccgccggtcg agatcgacgg acgctacttc gtcgacggcg cgttgcgcaa gacgctgcac
      241 gcctcgattg cactggacca gggtgtcgac ctgatgctct gcctgaaccc gctggtgccg
      301 ttcgattcga ccccgcacca ccgcacgcgc gaccgcgaac accagctcga caagctggtc
      361 gagggcggac tcccggtggt gctggcgcag acatttcgct cgatcatcca ttcgcgactg
      421 ggcgcggggc tggagcgcta caagacagcc tatccccaca ccgacattct gctgttcgag
      481 ccgaaccgcg cggatgccga catgttcttc accaacctgt tttcgtactc cagccggcgc
      541 cgcctgtgcg agcacgccta ccagaagacc cgcgaggagt tgtggatgcg gcggcacgaa
      601 ctggcgccac tcctccggcg ccacggcatc agcattcgca ccgacgtgct gcgcgacgag
      661 tcactgacgc tggtgaagag cctgcggcgc agccgcggca tctggcgcga gaccgcctcc
      721 accgccgcga cgcgcctgct gacccatacg ctggacgatc tcgaacgctg gcttcgctat
      781 tccagcgccg ctgcctaggg cctgttcacg atgcaggggc gtggcccgcc accgctgcgc
      841 tattcttgag ccggttcttc caccggcacc gctcgaaatc ccatggaacg caagccgccg
      901 cgtcgcactc gcgaacgcat tcttgagctg tccttgcgcc tgttcaacga cttcggcgag
      961 ccgaacgtca acacgacgct gatcgccgag gagatgaaca tctcccccgg caacctgtac
     1021 taccatttca agaacaagga cgacatcatc aactgcatct tccagcagtt cgaacgggag
     1081 atcgacaagt tgctggcgct gccgaccgac cgcgtgccaa atgtcgagga cgcctggctg
     1141 ttcctgcacg tgctgttcga actgatctgg aagtaccgct tcttctatcg cgacctcaac
     1201 aacctgctgg ccaacaaccg cacgctggaa ctgaagttca agcagctgct gaaccagaag
     1261 gtgaaggtgg cgcgcggttt gtgcgacgga ctggcgcagg ccggggagct gcaggcgggc
     1321 gcgcgcgaga tcgaggcgct cgccaccaac atggtggtgg tggcaaccta ctggctgtcg
     1381 tactcgtacg tccttgatcc gcgccgcttc aacgagcccg ctgtcatcgg cgctgcgttg
     1441 cagcgcggct gctaccaggt catggcgctg acatcgccct atctcaccgg ccagtcgcgg
     1501 gcgctcttcg atcagctcgc cgccgaatac ctcaacgact agacgaagga gacgcgatgg
     1561 cgaaggcgtg ggcgaagttc ccgtatcccg acaagaagta cgtgtacacc gccgcaaccc
     1621 tgaagaaagc ctgggaccgc ctgcacaagg gtgacgccga gccgttcccg aaggcagagg
     1681 ccgtcgtcga ggcctggatc gcgtttcatg ccggcgaatt ccagaaggcg tcggacctcg
     1741 gcctcaaggc cggtgcggaa ggcgccagcg tggcgaacaa ggcagagtcg atctacgcca
     1801 actacctcga ggacagcgac aagcgcaagc tcgagctctt cgaggaagtg gccgggcgcg
     1861 cggaggcact gcaagccaag gagcccagga acgccaacgc gttttactgg caggcgtact
     1921 cgctgggccg gtatgcgcag ggcatatcgg tcgggaaggc actcgcacaa ggcatcggcg
     1981 gcaaggtcaa ggtcgcactc gaaacgacga tcaagcttgc gcccaagcac ggcgatgcgc
     2041 acattgccct gggttcgtat cacgccgaag tcatcgacaa ggtcggcgcc atgatcggcg
     2101 gactgaccta cggcgcgaag aaggacgttg cgctgaagaa ctaccaggcg gcgctgaagc
     2161 tgatgccgga ttcggcgatc gcgcgtatcg agtacgccaa cgggctcgtc atgctcgaag
     2221 gcaagaaggc gctcaagcag gccgaggagc tgtatgaggc cgctgccgca tgcgagccga
     2281 tggacgccat ggagcgcctc gacgtcgaac tcgccaagtc cgaactcgag gactgaaccg
     2341 cggggacgag gcggaaccca gccgccgcct cattgccaca tggaagctac ttgccgtgcg
     2401 aggacttctt tcgcgcggac gccttgtgcg gctcctcggg cttcggcggg ttgccgacga
     2461 ctcccttgag cgggcagatc ttgaatgccg ggcatttttc acagccggcg acgatggcaa
     2521 tcggacaaag cgtcatggca gtcctccgct gcgcggctct cgcgctgatg cagccacctg
     2581 tgcgcaaacc ggcgtgggtg ccaccgtccg cgctcaggca atctcctcgt aaagcggcaa
     2641 cgtcaggaat tccgcaaagc cttcctgcgt gctcatcttc tcgaagatca cggcagcccg
     2701 atcatatgtg ggcgagtccg ggccgaccgc gcccttcact ttcgccagtt cttccggaat
     2761 caggcgacgg accaggtccg cggtgacctt gcggccatca tcgagcacgc ccttgggcga
     2821 acggatccat tgccaca
//
LOCUS       DATIRT010000097         1663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1683739, whole genome shotgun sequence.
ACCESSION   DATIRT010000097 DATIRT010000000
VERSION     DATIRT010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1683739"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..>1663
                     /gene="purF"
                     /locus_tag="VLN25_02400"
     CDS             219..>1663
                     /gene="purF"
                     /locus_tag="VLN25_02400"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HSQ09456.1"
                     /translation="MCGIVGVVATGPVNQLLYDSLQLLQHRGQEAAGIATGTGSTFSM
                     WKGNGMVREIFRTRNMRNLTGNVGIAHVRYPTAGSATSLEEAQPFYVNAPFGITLGHN
                     GNLTNAEQLKSEMFRIDRRHINTTSDSEVLLNVLAHELQEQSSSYTLDPATIFQAVAG
                     LHRRARGAYACVAHVAGHGILAFRDPFGIRPLCYGINETDGGTEYLVASESVALEGSG
                     FKFVRDVAPGEAVFIDLDGNFYAQQCAERFSLNPCIFEYVYFARPDSVIDGVSVYASR
                     LRMGEHLAEKIRREFRVGDIDVVMPIPDTSRPAAMQLAMKLGLEFREGFFKNRYVGRT
                     FIMPGQSVRRKSVRQKLNALPTEFKGKNVLLVDDSIVRGTTSREIVHMARESGANRVI
                     FASAAPPVRFPNVYGIDMPTRSELIAHGRSDEEIQRMIEADGLVYQDIDGMKRSVSEL
                     GPHLTSFEASCFDGHYITGDVTPEYLDRLEAT"
BASE COUNT          309 a          533 c          525 g          296 t
ORIGIN      
        1 tgccgcagtc cctggcacgc tatgcgtcga agccgggtcc gcagggaaac tagaatgaaa
       61 cgcacgtaca cctactttgc tggctgcctc ccggcgaagc gatcacgggc ccgcattcgg
      121 ccgggcgggc actaggatga actaccccca cgctcacttc gttcgctgcc ccccctgggg
      181 gcgctcagtg cccttcgggc ggccgggcgg gcactgaaat gtgcggaatc gttggcgtcg
      241 tcgcgaccgg gccggttaac cagctgctct acgactcgct gcaactgctg cagcaccgtg
      301 ggcaggaggc ggcgggcatt gccacgggga ccggcagcac cttctcgatg tggaaaggca
      361 acggcatggt gcgggagatc ttccgcacgc ggaacatgcg caatctcacc ggcaacgtcg
      421 gcatcgcgca cgttcgctat ccgacggccg gctcggccac gagtctggaa gaagcccagc
      481 cgttctacgt caacgccccc ttcggcatca cgctcgggca caacggcaac ctgaccaacg
      541 ccgagcaact caagagcgag atgttccgca tcgatcggcg gcacatcaat acgacctcgg
      601 actccgaagt gctgctcaat gtgctggcgc acgagttgca ggaacagagt tcgagctaca
      661 cactcgatcc ggccacgatc ttccaggccg tcgccggcct gcatcgtcgc gcgcgcggag
      721 cctatgcctg tgtcgcgcac gtggccgggc acggcatcct ggcgttccgg gatcccttcg
      781 gtatccggcc actgtgttac gggatcaacg agaccgatgg tggcaccgaa tacctggtcg
      841 cgagcgaatc cgttgcgctg gaaggatcgg gcttcaagtt cgtgcgcgac gtggcaccgg
      901 gcgaggctgt cttcatcgac ctcgatggca atttctacgc gcagcagtgt gctgagcgtt
      961 tctcgctgaa tccctgcatc ttcgagtacg tctactttgc gcgacccgat tcggtcatcg
     1021 atggcgtgag cgtgtatgca tcacgcctgc ggatgggcga gcacctggcc gaaaagatcc
     1081 ggcgagagtt ccgcgtcggg gacatcgacg tggtgatgcc gattcccgac acctcgcggc
     1141 cggccgcgat gcagctggct atgaaactcg ggctggagtt ccgcgaggga tttttcaaga
     1201 accgctacgt cggtcgcacc ttcatcatgc cgggacagtc ggtgcgccgg aagagcgtca
     1261 gacagaagct caatgcgctt cccaccgagt tcaagggcaa gaacgtgctg ctggtcgatg
     1321 attccatcgt tcgtggcacg acctcgcgcg agatcgtcca catggcgcgt gagtcgggtg
     1381 ccaaccgggt gatcttcgca tcggccgcgc cgccggtgcg cttccccaac gtgtacggca
     1441 tcgacatgcc gacacgcagc gaactcatcg cgcacggccg ctcggacgag gaaatccagc
     1501 gcatgatcga agccgatggc ctcgtctacc aggacatcga cgggatgaag cggtcagtgt
     1561 ccgagctagg cccgcatctg acgagcttcg aggcgagttg cttcgatggc cactacatca
     1621 cgggggacgt cacgccggag tacctcgatc gcctcgaagc gac
//
LOCUS       DATIRT010000098         5837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1487379, whole genome shotgun sequence.
ACCESSION   DATIRT010000098 DATIRT010000000
VERSION     DATIRT010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5837
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487379"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1069)
                     /locus_tag="VLN25_02405"
     CDS             complement(<1..1069)
                     /locus_tag="VLN25_02405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HSQ09457.1"
                     /translation="MSRLSILIVGGGPVGLTLALLLARQQIHSEVLDARTLDAACADR
                     RLLALSRGSVELIDPVVSLPSSVTAPIRSVVVSSAGEFGRVEIGAGDLGPRPLGLTVR
                     YGDLLAPLAAACGGNPFIRNTRPLRVTEYRQHSTKVLVRLDDGSEREAAILVNAEGTV
                     GPLPGDPAQVALVSDVVVQGPESGSAYERFTRDGPLALLPCPGAPSGVGRSMAMVWCM
                     PPGVAARRLALDDGAFTQELQQAFGVRNGTILHLGPRASYPLHQQSRAALREHRIVFV
                     GNAAQTLHPVAGQGLNLGLRDAAQLAGQLARAHAERRDPSTALADYEKMRRADRTAIV
                     ALTRNAPQLFATRAMPIALGRS"
     gene            complement(1083..1793)
                     /locus_tag="VLN25_02410"
     CDS             complement(1083..1793)
                     /locus_tag="VLN25_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HSQ09458.1"
                     /translation="MRALILAAGRGERMRPLTDDVPKPLLMAGDRRLIEWQLDRLARA
                     GIRDLVINIAHLPEQFEAVLGTGERYGVTLTYSREGGQADDALETLGGIVRALPLLDG
                     TPFVVVSGDIVTDFDYETLQRPAAAIIAGSADAHLVLVDNPAFHVHGDMGCVDGRIDP
                     DAPQRLTYANIGIFAPRLFKGLLPVRARLFPWLYAAARAGRVTGEHFRGRWYNVGTPA
                     ELARLDADLRTFGLEPSL"
     gene            complement(1944..2987)
                     /locus_tag="VLN25_02415"
     CDS             complement(1944..2987)
                     /locus_tag="VLN25_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HSQ09459.1"
                     /translation="MVLSTPPDSVPAPHDARRDQVIEWLRSLASRHAIEVESLQPASA
                     DASFRRYFRVQAGTGSLIVMDAPPDKEDVRPFVHVAGLLRAAGLNAPEVIEADEQQGL
                     LLLSDLGQDTYLNVLDERNAPGLFSDAIDALVRWQKATRPGVLPPYDDALLRRELQLF
                     PDWYVARHLGHELTGAQRESLAAMFDRIVASNLAQPVVYVHRDYMPRNLMVAAPNPGI
                     LDFQDAVIGPVSYDVASLFRDAFISWDEERVIDWTIRYWERARRAGLPVGTDFGTFWR
                     DFEWMGLQRHLKVLGIFARINYRDGKPHYLEDTPRFVGYVRAVARRYDELSPLARLLD
                     ELEGVATKVGYTF"
     gene            3122..5476
                     /locus_tag="VLN25_02420"
     CDS             3122..5476
                     /locus_tag="VLN25_02420"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HSQ09460.1"
                     /translation="MPRLPANAAPILRFALLPLSAALSVAVSTGALAQSVPAEAPTTP
                     AGAVPGQSRIPVSPDTPWPRLKLERMLSEKRLTGTEGAPTFARAHRIEGTIDEKIVLE
                     GNAEVRRGGTVLRGDRITYTQATDQVDVEGNARVFKQGGSFSGPELSFRIDAQTGAMP
                     DAQFTYPARSGRGDATLIEFLGDQRARMENARFTTCAPGDDAWWVQAESLEVDGLEQS
                     ATASGARLYFQGVPILVSPILGFPIGDQRRSGFLTPSFGLSTSVGTDIRTPFYWNIAP
                     HYDYTITPRAMSKRGVMLGNEFRFLQPTYSGTLLYDVIPYDRQTEETRDYTGVRFNYS
                     GPSGVAAGINYNRVSDDNYFVDFSSTILGSSQKVLPQDGFVSYTQTYWNTAVRVTKNQ
                     ILQDPLAPVTPPYERVPQVTVNGFVADWKGFEAAAILDGTRFTNPDAGLQSGSRYIFN
                     PRVAYPILSPGWFITPRAQLSATYYSLDPDFNPQDKSQSRVMPILSLDTGLIFERDTE
                     WFGRSSQQTLEPRLYYAYIPFRNQNQLPNFDSALADLNYAQFFTENIYSGYDRIVNAN
                     QMTLAIGTRVLDQETGAETLRAALGQRYYFSDQKVTLPGESPRSSNESDLLAALTAQL
                     GNYWAVDFAVQYSAEESAVSRATAGMRWQPRRASVLSAYYRYQESSIPGAGTSQTDIA
                     FQWPLSDRWYAVGRYNYSFESRKPVEVIAGFEFKEDCWLLRFAFQRFQTTTDSTTTNF
                     YVQLELSGLTSVGTNALSQLQRNIPGYQRINPLPRQPGMFEYYE"
     gene            5482..5787
                     /locus_tag="VLN25_02425"
     CDS             5482..5787
                     /locus_tag="VLN25_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09461.1"
                     /translation="MRMQFRASSWALFLLFVIALGARAQQPSATDVFRPVPGAPAAVQ
                     TAPSPAATSLPGTTTPATRTTRPVYRIVAVVNDEVITANELRALTLSAIAQLERQKI"
BASE COUNT         1063 a         1998 c         1825 g          951 t
ORIGIN      
        1 cagaacggcc gagcgcaatc ggcattgcgc gcgtcgcgaa gagttgcggc gcgttgcgag
       61 tcagcgcgac tatcgccgtc cggtccgcgc gccgcatctt ctcgtagtcg gccagcgcag
      121 tggacgggtc gcgccgttcc gcgtgcgcgc gcgcaagttg cccggcgagc tgcgctgcat
      181 cgcgcagacc aaggttcagg ccctgccccg cgaccggatg cagcgtctgc gccgcgttgc
      241 cgacgaagac gatccgatgc tcgcgcagcg cggcccgtga ctgctggtgc agcggatacg
      301 aagcacgtgg gccaaggtgc aggattgtgc cgttgcgaac tccgaaggcc tgctgcagct
      361 cttgtgtgaa ggctccgtca tccagcgcca ggcgacgcgc ggcgacaccg ggcggcatgc
      421 accacaccat ggccattgag cgccccacgc ccgacggcgc gccagggcac ggcagcagcg
      481 ccagcgggcc gtcgcgcgtg aaacgctcgt aggcgctgcc ggactcgggt ccctggacga
      541 ccacatcgga tacaagtgcc acctgcgccg gatccccggg cagtgggcct acggtgcctt
      601 cggcattgac gaggatcgcc gcttcgcgct cgctgccatc gtccagtcgc acgagcacct
      661 tcgtcgaatg ctggcggtac tccgtcacgc gcaggggccg cgtgttgcga atgaacgggt
      721 tgccgccgca ggcagcagcg agcggcgcga gcagatcgcc atagcgcacg gtcagtccga
      781 gcgggcgcgg tccgaggtca ccggcaccga tttccacacg tccgaactca cccgccgacg
      841 acaccaccac gctgcgtatc ggagccgtta ccgaagacgg cagcgagacc actggatcaa
      901 tcagctccac cgatccgcgc gacaaggcca gcagccgccg gtcggcacag gcggcatcca
      961 gcgtgcgcgc atcgaggacc tcgctgtgaa tctgctggcg cgcaagcagc agcgcgagcg
     1021 tcagaccgac gggtccgcca ccgacgatga gtatggacaa gcgcgacatg tcagtcacgg
     1081 ttctaaagcg acggctcgag gccgaaggtc cgcaggtccg catcgaggcg ggcgagctct
     1141 gcgggcgtcc cgacgttgta ccagcgtccg cggaagtgct caccggtcac acgccccgcg
     1201 cgggccgctg catagagcca tggaaaaagc cgcgcacgca ccggtagcag gcccttgaac
     1261 aggcgcggcg cgaagatgcc gatgttcgcg taggtcagac gctggggcgc gtcaggatca
     1321 atgcggccgt cgacacagcc catgtcgccg tgcacgtgaa aggcgggatt gtcgacgaga
     1381 accaggtggg cgtccgcgga gccggcgatg atggcagccg caggccgctg caacgtctca
     1441 tagtcgaagt ccgtcacgat gtcgccgctg accaccacga acggcgtccc gtcgagcagc
     1501 ggcaacgcgc gcacgatccc gcccagggtc tccagcgcat cgtccgcctg cccgccttcg
     1561 cgcgaatagg tgagagtgac accgtagcgt tcgcccgttc ccagaaccgc ctcgaactgc
     1621 tccggcaggt gcgcaatgtt gatgacgagg tcgcggatgc cggcccgtgc aagccggtcc
     1681 agttgccatt cgatcaggcg acgatctccg gccatcagca gcggcttggg cacgtcatcc
     1741 gtcagcggcc gcatccgctc tccgcgtcct gcagccagta tcagtgcgcg cacgtcatcc
     1801 gcctcaagtc ctgtgacagg ccgcaatgca gacagcctgc ttttgtattc gagtcgggcg
     1861 cacggccgct ccgaagcccg actcgccagt cttcccggcc catgtgcacg tgcatcgcaa
     1921 agcgatgcgt acacatgggc cgcctaaaac gtatagccca ccttcgtcgc cacgccttcc
     1981 agttcgtcga gcaatcgcgc cagcggcgag agttcatcgt aacggcgcgc gactgcgcgc
     2041 acgtagccga caaagcgcgg cgtgtcctcc agatagtgcg gcttgccgtc gcgatagttg
     2101 atgcgcgcga agatgccgag caccttcagg tgccgctgca ggcccatcca ctcgaagtcg
     2161 cgccagaatg ttccgaaatc ggtgccgacc ggcaggccgg cgcgacgcgc acgctcccag
     2221 tagcggatcg tccaatcgat cacgcgttcc tcatcccagc tgatgaaggc atcccgaaac
     2281 agggaggcca cgtcatacga caccggcccg atcacggcat cctggaaatc aagaatgccg
     2341 ggattcggcg ccgcgaccat caggttgcgc ggcatgtagt cgcgatgcac gtagaccacg
     2401 ggctgcgcca ggttcgacgc gacaatccga tcgaacatcg ccgcgaggct ctcgcgctgc
     2461 gcacctgtca gttcatgccc gagatgccgc gcgacgtacc agtcgggaaa gagctgcagc
     2521 tcgcgccgta gcagcgcgtc gtcatagggc ggaagtacgc ccggccgcgt cgccttctgc
     2581 catcgcacca gggcatcgat ggcgtccgaa aacaggcccg gggcattgcg ttcgtcgagc
     2641 acgttcagat acgtgtcctg gccaagatcg ctcagcagca gcaggccctg ctgctcgtcg
     2701 gcttcgatca cctccggcgc attcagcccc gcggcgcgca gcagtccggc gacgtgtacg
     2761 aaaggacgca cgtcttcctt gtcgggcggc gcgtccatca cgatcaacga gcccgtgccg
     2821 gcctgcacac ggaagtagcg gcggaagctc gcgtcggccg atgcgggttg aagggactcc
     2881 acttctatcg cgtggcgcga cgccagcgac cgcagccact cgatcacctg atcacggcgc
     2941 gcgtcatggg gagcaggtac ggagtcggga ggggttgaaa ggaccatggc gtgcgaatga
     3001 tgtcagaccg cagcgaccga ctgcgcggcc tcagtagatg cgatgcctat aatcggcgcg
     3061 cctttcacgg cccttaatgg tcactactgg ctcacggctc gacctcgcca tcgaattcta
     3121 aatgccgcgc ctgccggcga atgccgcgcc gatcctgcgt ttcgcactgc taccgctgag
     3181 cgctgcgctg tccgttgctg tatccacggg cgccctggcg cagtcggtac ctgctgaagc
     3241 gccgaccacg ccggccggag cagtgcccgg gcagtcccgc attccggtca gtcccgatac
     3301 gccctggccg cggctcaagc tcgaacgcat gctgtccgag aagcggttga caggaacgga
     3361 aggcgcgccc accttcgcgc gcgcccaccg cattgaaggc acgatcgacg agaagatcgt
     3421 gctcgagggc aatgccgaag tgcggcgtgg cggcaccgtg ctccgtggcg atcgcatcac
     3481 ctacacgcag gccaccgacc aggtcgacgt cgaaggcaac gcacgggtgt tcaagcaggg
     3541 cggatcgttc tcgggacccg agctgtcgtt tcgcattgac gcgcaaactg gcgccatgcc
     3601 cgatgcccag ttcacctatc cggcacgcag tggtcgtggt gacgccacgc tgatcgagtt
     3661 cctcggggac cagcgcgccc gcatggaaaa tgcccgcttc acgacctgtg cgccggggga
     3721 cgatgcctgg tgggtgcagg ctgaatcgct ggaggtcgat ggactggagc agtctgccac
     3781 cgccagcggc gcgcggctct acttccaggg cgtgccgatc ctcgtctcgc cgatcctcgg
     3841 attcccgatc ggcgaccagc gccgctccgg gttcctgacg cccagtttcg ggctgagcac
     3901 atccgtgggc acggacatcc ggacaccgtt ttactggaac atcgcacccc actacgacta
     3961 cacgatcacg ccgcgcgcga tgagcaagcg cggcgtgatg ctcggcaacg agttccgctt
     4021 cctccagccg acctatagcg gcacgctgct ttacgacgtc atcccctacg accggcagac
     4081 cgaagagaca cgcgattaca ccggcgttcg gttcaactat tcgggaccat cgggtgtcgc
     4141 cgccggcatc aactacaacc gtgtgtccga cgacaactac ttcgtcgact tttcgagcac
     4201 gattctcggc tcgtcgcaga aggtgttgcc gcaggacggg tttgtctcct acacgcaaac
     4261 gtactggaac accgcggtcc gggtcaccaa gaaccagatc ctccaggacc cgctggcgcc
     4321 ggtcacgcct ccgtacgagc gcgtgccgca ggtcacggtc aatggattcg tggccgactg
     4381 gaagggcttc gaggcggcgg cgatccttga cggaacccga tttacgaatc ccgatgcagg
     4441 cctgcagtcc ggatcgcgct acatcttcaa tccgcgcgtt gcctatccga tcctgagccc
     4501 gggctggttc atcacgccgc gtgcgcagct ttcggccacc tattacagcc tcgacccgga
     4561 cttcaatcca caggacaaat cgcagtcgcg cgtgatgccg atcctgagcc tcgataccgg
     4621 cctgatcttc gagcgcgaca cggagtggtt cggccgctcg tcgcagcaga cactggaacc
     4681 gcggctctac tacgcctaca tcccgttccg caaccagaac cagctgccca acttcgacag
     4741 cgcgctggcc gacctcaact atgcgcagtt cttcaccgag aacatctact cgggctacga
     4801 ccgcattgtc aacgccaacc agatgacgct ggcgatcggc acgcgcgtgc tcgaccagga
     4861 aaccggtgca gagacgctgc gcgcggctct gggccagcgt tactactttt ccgaccagaa
     4921 ggtcacattg cccggagagt caccgcgttc cagcaacgag agcgacctgc tggccgccct
     4981 cacggcgcag ctgggcaact actgggcagt ggacttcgcc gtgcagtact ccgcggagga
     5041 gagtgcggtc tcacgtgcca ccgccggcat gcgctggcag ccccggcggg catcggttct
     5101 gagcgcgtat taccggtacc aggaaagcag catcccggga gccggcacca gccagacgga
     5161 catcgcgttt cagtggccgt tgtccgatcg ctggtacgcg gttggtcgct acaactattc
     5221 gttcgaaagc cgcaagccag tggaggtgat tgcgggtttc gagttcaagg aggactgctg
     5281 gctgctgcgc ttcgcgttcc agcgcttcca gacgaccacg gactcgacga cgaccaactt
     5341 ctacgtccag ctggaactgt cgggcctcac cagtgtcgga accaatgccc tctcgcaact
     5401 gcagcgtaat atccccggct accagcgcat caacccgctg ccacggcagc cgggtatgtt
     5461 cgagtactac gagtagatcc catgcgtatg caatttcgcg cctcatcgtg ggcgctgttc
     5521 ctactttttg tgatagccct cggcgcgcgc gcacagcagc catcggccac cgacgtgttc
     5581 cgtccagtac ccggtgcgcc ggcggcggtg cagaccgcac cttccccggc ggcaacctcg
     5641 ctgccgggca ccacgacccc ggcaacgcga acgacgcggc ccgtttaccg catcgtcgcc
     5701 gtcgtcaatg acgaggtgat caccgccaat gagctgcgcg ccctcacgct gtcggcaata
     5761 gctcagctgg agcggcagaa gatctagctg cccccgccgg atgtcctcga gcggcaggtc
     5821 ctggaacgca tgatcgt
//
LOCUS       DATIRT010000099         5250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1585726, whole genome shotgun sequence.
ACCESSION   DATIRT010000099 DATIRT010000000
VERSION     DATIRT010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585726"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VLN25_02430"
     CDS             complement(<1..761)
                     /locus_tag="VLN25_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSQ09462.1"
                     /translation="MGTLLPRLSQNRSSVWLATAAIAIVLLLLPFIVGQLGNAWVRAL
                     AFACLYVMLALGLNIVVGFAGLLDLGYIAFYAVGAYMYALLASPHLTSTFPWIAQMFP
                     TGLHNSIWVVIPLGAGIAATFGIILGAPVLKLRGDYLAIVTLGFGEIVRIFLNNLNAP
                     VNITNGPQGITLIDPITIAGVNFGKTQNLFGISLPSVYLYYYLFLVLTIITIVICVRL
                     QDSRLGRAWMAIREDEIAAKAMGINVRNVKLLAFSL"
     gene            complement(764..1693)
                     /locus_tag="VLN25_02435"
     CDS             complement(764..1693)
                     /locus_tag="VLN25_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSQ09463.1"
                     /translation="METFIQQILNGLVLGSMYSLIALGYTMVYGILNLINFAHGDVLT
                     VGALTALSTILVLQALFPGLPGWVLISAGLLAAIPVCVVLSLVIERVAYRPLRNAPRL
                     APLITAIGVSIVIQTVAMLVWGRNYLTFPQLITNTPIEFGGATVTVTKLLVIALSAVV
                     MVGLMLLVNRTKLGRAMRATSENPRVAGLMGVNPNFVIAATFAIGAALAALAGVMLAA
                     TYGVAHFYMGFLPGLKAFTAAVLGGIGNIPGAMLGGLLLGLIEALGAGYIGQLTGGVL
                     GSHYQDVFAFAILVLVLIFRPSGLLGERVADRA"
     gene            complement(1827..3017)
                     /locus_tag="VLN25_02440"
     CDS             complement(1827..3017)
                     /locus_tag="VLN25_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HSQ09464.1"
                     /translation="MNHRIIKLVPLAIAAAVALAACGKKEEAPKAAAPKAAAAAPQKG
                     QIKIGHVAPLTGGIAHLGKDNENGAKLAAKEINAAGGVKIGDQVFEIVILGEDDKADP
                     KEGTLAAQKIVDSGAVAVVGHLNSGTSIPASKIYSDAGMTQISPSATNPKLTEQGFKT
                     TFRVVANDNQQGAVLANYANDALKAKTMAIVDDRTAYGQGLADVVEKVAKEKGIKIVA
                     REFATDKTTDFNAILTKIRGAKPDVVMYGGMDATAGPMAKQMKQLGLKSVLLAGDGVC
                     SPEFIKLAGDAAGIMHCSMAGEAVEKLAKGEEFKAKYKAEFGQEVQIYSPYSYDAVYI
                     ITDAIKRAGKADRAAISAAVPATNYPGLTGTIMFDEKGDIKNGAISMFKVSGDKLQYL
                     STVR"
     gene            3298..4554
                     /locus_tag="VLN25_02445"
     CDS             3298..4554
                     /locus_tag="VLN25_02445"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019478 - D-amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HSQ09465.1"
                     /translation="MRVLVLGAGVVGVASAWFLREAGHDVVVVDRQPGPALETSFANG
                     GQISASHAEPWANPAAPGRILRWIGQPDAPLLFRPRADLQQWLWCAAFLRECRSSRTA
                     ANIRTILALALHSRDVVKRLRRELALEYDCVERGILHFYTDRREFEASRAPAALMRTL
                     GCHRRTISAAEAVVLEPALAPVEHRIVGADYCAEDESGDAHRFTQQLAQRCVERGVQF
                     RFGSTATSILLADGRARGAVLRDAQGDLSEQRADATLVCLGVESVALLRSVGQRLMIY
                     PGKGYSATFDISDPSKAPPLSLTDDEFKLVLSRFGSRLRVAGTAEIAGWGTELDPLRC
                     EALTRRTAQLFPGACDYDRPNYWAGRRPVTPSNVPYLGHARIAGLFLNTGHGTLGWTL
                     GPGNGELIAQAISGTATHVPLPADRR"
     gene            complement(4622..4927)
                     /locus_tag="VLN25_02450"
     CDS             complement(4622..4927)
                     /locus_tag="VLN25_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSQ09466.1"
                     /translation="MKHVLVLAALGITIASGPVFASPELAKQKNCLACHAADKKLVGP
                     SYKDVAAKYKGDKDAFAKIAKKIREGGVGVWGQVPMPANPQVNEQEAMTLAKWVLSQ"
BASE COUNT          894 a         1645 c         1749 g          962 t
ORIGIN      
        1 agcgagaacg cgagcagctt gacgttgcgc acgttgatgc ccatcgcctt ggccgcgatc
       61 tcgtcctcgc ggatcgccat ccaggcacgg ccgaggcggg aatcctgcag ccgcacgcag
      121 atgacgatcg tgatgatcgt cagcaccagg aacaggtagt agtagagata cacggacggc
      181 aggctgatgc cgaagagatt ctgagttttg ccgaaattga cgcccgcgat cgtgattgga
      241 tcgatcagcg tgatcccctg cggcccgttg gtgatgttca ccggggcatt caggttgttc
      301 aggaagatgc gaacgatctc gccgaagccg agcgtcacga tggcaaggta gtcgccgcgc
      361 agcttgagta ctggcgcacc gaggatgatg ccgaacgtcg ctgcgatccc ggcgccgagc
      421 ggtatcacga cccagatcga gttgtgcaac ccggtcggaa acatctgcgc gatccagggg
      481 aacgtactgg tgaggtgcgg cgaggcgagc agtgcgtaca tgtaagcccc gaccgcatag
      541 aacgcgatgt agccgaggtc gagcagcccg gcgaagccga cgacgatgtt caggccgagt
      601 gccagcatca cgtacagaca tgcgaaggcg agcgcgcgga cccatgcatt gccgagctgc
      661 ccgacgatga atggcagcag cagcagcacg atggcgatcg ccgccgtcgc cagccagaca
      721 ctggaacggt tctgcgacag gcgcggcagc agcgtgccca tcgtcaggcg cggtcggcga
      781 cgcgttcgcc gagcagtccg gacggccgga agatcagcac cagcaccagg atggcgaacg
      841 cgaacacatc ctggtagtgg ctgccaagca cgcctcccgt cagctgtccg atatagcccg
      901 cgcccaatgc ctcgatcaac ccgagcagca atcccccgag catcgccccc gggatgttgc
      961 cgatgcctcc gagcacggcg gcggtgaatg ccttgagacc gggcaaaaag cccatgtaga
     1021 agtgcgcgac gccgtaagtc gccgccagca tcacgccggc gagcgccgcc agcgcagcgc
     1081 cgatcgcgaa cgtcgccgcg atcacgaaat tggggttcac gcccatgaga cccgcaacgc
     1141 gcggattttc cgatgtagcg cgcatcgcgc ggccgagctt ggtgcggttg acgagcagca
     1201 tcaggcccac cataacgacg gccgatagcg cgatcacgag cagcttggtg accgtgactg
     1261 tggcgccgcc gaactcgatc ggcgtgttcg tgatcaactg cggaaaggtc aggtagttgc
     1321 ggccccagac gagcatcgcc acggtctgga tgacgatcga gacaccgatt gcggtgatca
     1381 gcggcgccag gcgcggagcg ttgcgcagcg gtcgataggc cacacgctcg atgaccaggc
     1441 tcagcacgac gcagaccgga attgccgcca gcaggccggc cgagatcagc acccaacccg
     1501 gcaggccggg gaacagtgcc tgtagcacga gaatcgtcga cagggcagtg agcgcgccga
     1561 ccgtcagtac atcgccgtgc gcgaagttga tcaggttcag gatcccgtag accatcgtgt
     1621 agccgagcgc gatcagcgaa tacatgctgc cgagcacgag gccgttgagg atctgctgga
     1681 taaacgtttc catcgacgcc ttttgtaagc ggctggacgc gctgcctgac cgcgcgatcg
     1741 cgcatcgtag caaggcgcct gatgcgcgcc gggatgcgca aagaaaacgg cacccgaggg
     1801 tgccgttttc gtggagccga cgggatctag cgaaccgtcg agaggtactg gagcttgtcg
     1861 ccggagacct tgaacatcga gatggctccg ttcttgatgt cgcccttctc gtcgaacatg
     1921 atcgtgccag tcaggcccgg gtagttcgtc gccggcaccg cggccgagat cgcggcgcga
     1981 tcggccttgc cagcgcgctt gatcgcgtcg gtgatgatgt acaccgcgtc gtaggagtac
     2041 ggcgaataga tctgcacttc ctggccgaat tccgccttgt acttggcctt gaactcctcg
     2101 cccttcgcga gcttctcgac cgcctcaccg gccatcgagc agtgcatgat cccggccgcg
     2161 tcgccggcca gcttgatgaa ctcgggcgag caaacgccgt cacccgccag cagcaccgac
     2221 ttcaggccga gctgcttcat ctgcttggcc atcgggccgg cggtggcgtc catgccgccg
     2281 tacatgacga cgtcgggctt ggcgccgcgg atcttggtca ggatggcatt gaagtccgtc
     2341 gtcttgtcgg tggcaaattc gcgcgcaacg atcttgatac ccttttcctt cgcaaccttc
     2401 tcgaccacgt cggccaggcc ctggccgtag gcggtacggt catcgacgat cgccatggtc
     2461 ttggccttga gcgcgtcgtt agcgtagttg gcgagcacgg cgccctgctg gttgtcgttg
     2521 gcgacgacgc ggaacgtggt cttgaaaccc tgctcggtca gcttcgggtt cgtcgccgac
     2581 ggcgagatct gggtcatgcc ggcgtcggaa tagatcttcg aggccgggat cgacgtgccc
     2641 gagttcaggt ggccgacaac agcaacagcg ccggagtcga cgattttctg cgcggcgagc
     2701 gtgccttcct tcgggtcggc cttgtcgtct tcaccgagga tcacgatctc gaagacctgg
     2761 tcgccgatct tgacgccgcc ggccgcattg atttccttgg ctgcgagctt ggcgccgttc
     2821 tcgttgtcct tgcccaggtg cgcgataccg ccggtcagcg gggccacgtg gccgatcttg
     2881 atctggccct tttgcggggc cgcagccgcg gccttggggg cggcggcctt gggggcttcc
     2941 tctttcttgc cacaagcggc aagcgcgacc gcggcggcaa ttgccagcgg cacaagcttg
     3001 ataatccggt gattcatgac cttttctcct ctatgagtgt cggccgactg gtgatgtgtc
     3061 cttgggaact gttcttgggg acgtaggccc ggtgattctt cgcggcggca cccgttactt
     3121 gtgacaggaa cgctagcctg cgaaagcgca ggattgtact caggtacgaa cctcctgcaa
     3181 ggcggcatcg gggtctgcat gccggcgctg tgcggatcac gaagagtcga ggccaccggt
     3241 acttgccagc ggtactcctt agaatcgcgc tttgccgacc acatctggat caggaacgtg
     3301 cgcgtactcg tcctcggcgc cggcgtcgtc ggtgtggcca gcgcgtggtt cctgcgggaa
     3361 gccggccacg acgtcgtcgt agtggatcga cagcccggcc cggcgctcga aacgagcttc
     3421 gccaacggcg gacagatctc cgccagccac gccgaaccct gggccaatcc ggccgctccg
     3481 ggcaggatcc ttcgctggat cggccagccg gatgcgccgc tgctgttccg gccgcgtgcc
     3541 gacctgcagc agtggctctg gtgtgccgcc ttcctgcgcg agtgtcgctc gtcccgcacg
     3601 gccgcgaaca tccggacgat tcttgcgctg gctctgcaca gtcgtgatgt tgtcaagcgc
     3661 ctgcgccgcg agcttgcgct cgagtacgac tgtgtcgagc gcggcatcct gcatttctat
     3721 accgatcgcc gcgaattcga ggcgtcgcgg gcgccggcgg ccctgatgcg cacgctgggc
     3781 tgccaccgcc gcacgatctc ggcggcggaa gcggtcgtgc tcgagccggc gctggcaccg
     3841 gtcgagcacc ggatcgtcgg tgcggactac tgcgccgagg atgagtccgg cgatgcacac
     3901 cgattcacgc agcagctcgc gcagcgctgc gtcgagcggg gcgtgcagtt ccggttcgga
     3961 tcgaccgcga cttcgatcct gctggccgac ggacgcgcgc gcggtgccgt gctgcgcgac
     4021 gcccagggcg acctgtccga gcagcgggca gatgcgacgc ttgtgtgcct cggcgtggag
     4081 tcggtagcgc tgctgcgcag tgtgggccag cgcctgatga tttatccggg caaggggtat
     4141 tcggcaacgt tcgacatcag cgatccctcg aaggcgcccc cgctcagcct gaccgacgac
     4201 gagttcaaac tggtgctgtc ccgcttcgga agccggttgc gcgttgcggg aaccgccgag
     4261 atcgcgggtt ggggcacaga gctggatccc ctccgctgcg aggccctgac gcgacgcacg
     4321 gcgcagctct ttccgggggc ttgcgactat gacaggccga actactgggc ggggcggcgc
     4381 ccggtcacgc cgtcgaacgt gccgtatctg gggcatgccc ggatcgccgg actgttcctg
     4441 aacaccgggc acggtacgct gggctggacg ctcgggccgg gaaacggtga gctgattgcg
     4501 caggccatct cgggcaccgc gacccacgtt ccgctgcctg cggaccgccg ctgagctgcg
     4561 ccgacgaccg gggaaataaa aaggggcgcc gcagcgcccc tcgtgcttcg ccgacagccg
     4621 actactgcga caggacccac ttcgcgagcg tcatggcttc ctgctcgttg acttgcgggt
     4681 tcgcgggcat cgggacctgg ccccagacgc cgacgccgcc ttcgcggatc ttcttggcga
     4741 tcttggcgaa cgcgtccttg tcgcctttgt acttcgcggc gacgtccttg taggacggtc
     4801 cgaccagttt cttgtcggct gcgtggcagg ccaggcagtt cttctgcttg gcgagttccg
     4861 gcgaggcgaa caccgggccg ctggcaatag tgatgccaag ggcggcaagg acgaggacat
     4921 gcttcattgg gggtctccgt aagggtctgt gatgaggggc tgtaaaaggc cgctaattcg
     4981 atagggttct ggggaaacga ttccgggtaa acgcctgaag attctaacgg cttgcccgct
     5041 tttacgttga ctgcccacct tcagttgcgg ggcgggcgcg actatagtgg cctagtcgaa
     5101 tctgcgggag aaagcccgtg tggctgctct ggctcggtgt tgcgctggtc attctcaagc
     5161 tgctcgagat cagcgttttc gcggacctgt catggtggtg gatcaccgtg ccgttcgtcc
     5221 tgtcgatgat ctggttcgag atcttcgagc
//
LOCUS       DATIRT010000100         2388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_700965, whole genome shotgun sequence.
ACCESSION   DATIRT010000100 DATIRT010000000
VERSION     DATIRT010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700965"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VLN25_02455"
     CDS             <1..306
                     /locus_tag="VLN25_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HSQ09467.1"
                     /translation="QREQVLGTVEIRQIFRVPKIGAVAGCMVLDGLVKRTASARLLRD
                     SVVIWTGELDSLKRFKDDVKEVKAGFECGLSLKGYDDIKEGDQLEIFEVQEVARTLL"
     gene            400..759
                     /gene="rbfA"
                     /locus_tag="VLN25_02460"
     CDS             400..759
                     /gene="rbfA"
                     /locus_tag="VLN25_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HSQ09468.1"
                     /translation="MKPTRKPGRGLRIADQIQRDLAEMIQREVSTERAGLITLTGVEV
                     TPDYAHAKVYFTTIGAPLDAAQHVLNEKAGYFHSLLFKRLSIHTIPKLTFVHDASVEH
                     GIEIDRLIAEANRSQSE"
     gene            773..1684
                     /gene="truB"
                     /locus_tag="VLN25_02465"
     CDS             773..1684
                     /gene="truB"
                     /locus_tag="VLN25_02465"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HSQ09469.1"
                     /translation="MVKPRSERVDGVLLLDKPGGMTSNRALQGARRLLGAAKAGHTGT
                     LDPMATGLLPLTFGEATKFAADLLDADKSYRATVRLGMTTTTGDAEGEVVATAPVEVT
                     AAEVDAVLSRFTGTIAKVPPMYSALKRDGKPLYQLARAGIEVERAPRPVSIHRLERVE
                     HVGHDLILEVDCSKGTYVRVLAEDIGRALGCGAHLAALHRTRVGRLTLEGAVTLQTLE
                     DMPAEARRRCLQPVDTLVASLPRIELDDPLAGRFRQGQRLAIEAQPRGARVRVYAPHE
                     ELLGTANVSEWGVVAPERLISNESTQS"
     gene            1681..>2388
                     /locus_tag="VLN25_02470"
     CDS             1681..>2388
                     /locus_tag="VLN25_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HSQ09470.1"
                     /translation="MKRQLRNVAIIAHVDHGKTTLVDKLLQQSGTFRENQQVAERVMD
                     SNDIERERGITILAKNCAVDYEGTHINIVDTPGHADFGGEVERVLSMVDGVLLLVDAV
                     EGPMPQTRFVTRKALAQGLKPIVVVNKIDRPGARPDWVVNHMFDLFDKLGATEEQLDF
                     PVIYASALNGYAGTSPDVRSGDLRPLFEAILAHVRVRDDDANGTLQLQICSLDYSSYV
                     GKIGIGRITRGRLRAGQE"
BASE COUNT          457 a          698 c          832 g          401 t
ORIGIN      
        1 cagcgcgagc aagtgctggg aaccgtcgag atccgtcaga tcttccgtgt accgaagatc
       61 ggcgcggtgg ccggctgtat ggtgctcgac ggcttggtca agcgcacagc gagcgcgcgc
      121 ctgctgcgcg acagcgttgt catctggacc ggcgagctcg actcgctcaa gcgattcaag
      181 gatgacgtca aggaggtcaa ggcgggcttc gaatgcggcc tcagcctcaa gggctatgac
      241 gacatcaagg aaggcgatca gctggaaatc ttcgaagtcc aggaagtcgc ccggaccctg
      301 ttgtagcaat gccggcgaaa gtaccaacct gctttcgctg ggtcgagttt gaaaaagttc
      361 gctggcagag ccagacgaac tttttgatgg cacctgcata tgaaaccaac caggaagccc
      421 gggcgcgggc tgcgcatcgc ggatcagatc cagcgcgacc tcgcggagat gatccagcgc
      481 gaggtgtcta ccgagcgcgc cggactgatc acgttgaccg gagttgaagt gactccggac
      541 tacgcgcatg caaaggtgta cttcacgacg atcggtgcgc cgctcgatgc ggcccagcat
      601 gtgctgaacg agaaggcggg gtacttccac tcgttgttgt tcaagcggct gtcgattcac
      661 acgattccga agctgacgtt cgtgcacgat gcgtcggtcg agcacggcat cgagatcgat
      721 cggttgatcg ccgaagcgaa ccggagccag tccgagtagg acgggaggtc gcgtggtcaa
      781 gccgcgcagc gagcgcgtcg atggggtcct gctgctcgac aagccgggcg gtatgacgtc
      841 gaaccgcgcg ctgcagggtg cgcgacggtt gctgggtgcc gccaaggcgg ggcataccgg
      901 cacgctggac ccgatggcca ccggcctgtt gccgctgacc ttcggcgagg cgacgaagtt
      961 cgccgcggac ttgctcgacg ccgacaagtc ctaccgggcc actgtccgac tcggcatgac
     1021 gacgacgacg ggggacgccg agggtgaagt ggttgcgacg gcgcccgtcg aagtgacggc
     1081 cgcggaagtc gacgcggtgt tgtcacggtt caccggcacc atcgccaagg tgccgccgat
     1141 gtactcggcg ctgaaacgcg acggcaagcc gttgtatcag cttgcccgcg ccggaatcga
     1201 agtcgaacgg gcgccgcgac cggtgtcgat acatcggctt gagcgcgtcg agcatgtcgg
     1261 ccatgacctg atactcgagg tcgactgcag caagggcacg tatgtgcggg tcctggccga
     1321 ggacatcggg cgcgcgctgg gttgcggcgc gcatctggcg gccctgcatc gcactcgcgt
     1381 cgggcggctg acgctcgagg gcgccgtgac gcttcagacg ctggaggaca tgccggcgga
     1441 agcgcggcgg aggtgcttgc agccggtcga cacgctggtt gcaagtttgc cgaggatcga
     1501 gctggacgac cctctggccg ggcgcttccg gcaggggcag cggttggcga tcgaggcgca
     1561 gccgcgcggg gcccgggtac gggtgtacgc gccgcacgaa gaactgctgg gaacagcgaa
     1621 cgtcagcgag tggggcgtcg ttgcaccgga acgattgata tcgaacgaaa gtacacagtc
     1681 atgaaacgcc aattgcgcaa cgtcgcgatc atcgctcacg tcgatcacgg caagaccacg
     1741 ctggtggaca agctgctgca gcagtccgga accttccgcg aaaaccagca ggtcgcggag
     1801 cgggtgatgg actcgaacga cattgagcgc gagcgtggaa tcacgatcct cgcgaagaac
     1861 tgcgcggtcg actacgaggg tacgcacatc aacatcgtcg atacgccggg tcacgcggat
     1921 ttcggtggtg aagtggagcg cgtgttgtcg atggtggatg gcgtgctgct gctggtcgac
     1981 gctgttgaag gcccgatgcc gcagacgcgc tttgtcacgc gcaaggcgct cgcccagggg
     2041 ctgaagccga tcgtcgtggt caacaagatc gaccggcccg gcgcacggcc ggactgggtc
     2101 gtgaaccata tgttcgatct cttcgacaag cttggcgcga ccgaagagca gctcgacttt
     2161 cccgtgatct acgcgtcggc gctcaatggc tatgcgggca cgagtcccga tgtgcgcagt
     2221 ggtgatctac ggccgctgtt cgaggcaatc ctcgcgcatg tgcgcgtgcg cgacgacgac
     2281 gccaatggca cgctgcaact gcagatctgt tcgctcgact attcgagcta cgtcggcaag
     2341 atcggcatcg gccgcatcac gcgcggacga ctgcgggcgg gacaggaa
//
LOCUS       DATIRT010000101         2742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1192772, whole genome shotgun sequence.
ACCESSION   DATIRT010000101 DATIRT010000000
VERSION     DATIRT010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192772"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="VLN25_02475"
     CDS             <1..1031
                     /locus_tag="VLN25_02475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HSQ09471.1"
                     /translation="AGVVLVALLAACSTTPPGSAPTKKGAYYQDDGPPERVPADIAAI
                     PDAVPRVEPPHRAASRSYVALGRTYTPLTGDVSFRQRGMASWYGRQFHGNRTANGEIY
                     DMFGMTAAHPILPLPSYVRVTNIRNGRSVIVRVNDRGPFKHDRVIDLSYAAATKLGIA
                     AAGTGEVEVERITMTQIASGEYRRGDTEATRSASEMPMTTPVTPTSVAPISVAQSASS
                     NPTSSVVVPTGLTVPVVSGVPIESTPAREAPSTSTGSAGSPRGWAVQLGAFSQEVNAE
                     ALAGRTAALLAFLEPADALPVRVPRIEKDGAVYRVLVGATPDRDAALSLARELERVLD
                     RPTTLLLR"
     gene            complement(1061..1972)
                     /gene="rsmI"
                     /locus_tag="VLN25_02480"
     CDS             complement(1061..1972)
                     /gene="rsmI"
                     /locus_tag="VLN25_02480"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HSQ09472.1"
                     /translation="MGPGASHPRHDDLTALAGLAEQDLPVRALYVVALPIGNAADITL
                     RALWTLARVDAIAAEDTRVTGPLLARFGIATPLLAAHQHNERAVAAAIVDRLQQGQRV
                     ALVSDAGTPGVSDPGAVIVRAVLEADLRVIPIPGASSALAAVSAVGLRVTAFRFIGFL
                     PAGARERERLLRDIALSDGAAVLFEAPHRIRALQGDLSHILTPDRRVVIARELTKKFE
                     TIRVHTASALADLEPEERGEYVIVIDAAEQEARPALDPQGQRWLDALLEEMTPARAAA
                     VVSRMTGVPRSTVYDEAIGRRRKAPPE"
     gene            2127..>2742
                     /locus_tag="VLN25_02485"
     CDS             2127..>2742
                     /locus_tag="VLN25_02485"
                     /inference="COORDINATES: protein motif:HMM:NF016251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HSQ09473.1"
                     /translation="MLLPAQSTAFALPAEALREGFFAAHKVGGDDIAIQVVELDDSAD
                     QLGVALASARDRGVGIVVGPLPRTAVTDLVEGQRASLPLLALNFPESDGAAPTSMLAM
                     ALSVEYEAQRIARLALSEFVGVRTADTRPRIAVVTRPGGIERRIAQAYVSALRSQGEV
                     PQLVEWTPATAGKVAAQLTTSTLEAVFLALTARDAAQMRALIPRQ"
BASE COUNT          430 a          929 c          932 g          451 t
ORIGIN      
        1 cggcgggcgt ggtgctcgtg gcgctgctgg cggcctgtag tacgactcca ccgggctcgg
       61 cgccgacgaa gaagggcgcc tactaccagg acgatggccc gcccgagcgc gtgccggccg
      121 acatcgccgc gataccggac gccgtgccgc gcgtcgagcc gccgcatcga gcggccagcc
      181 ggtcgtatgt cgcattgggc cgcacctata ccccgctgac cggtgatgtg tcgtttcgcc
      241 agcgcggcat ggcgtcgtgg tacggccggc aatttcatgg caaccgcacc gcgaacggcg
      301 agatctacga catgttcggc atgactgcgg cacatccgat cctgccgctc ccgagctatg
      361 tgcgtgtcac caacatccgc aatggccgta gcgtcatcgt ccgtgtcaac gatcgagggc
      421 ccttcaaaca cgaccgcgta atcgacctgt cgtacgccgc agcgaccaag ctcggcattg
      481 ctgccgcggg gaccggagaa gtcgaggtcg agcgcatcac catgacgcag atcgccagtg
      541 gcgagtatcg acgcggcgac actgaagcga ctcgcagcgc cagcgagatg ccgatgacga
      601 ctccggtgac cccaacttcg gtggccccga tttcagtggc ccaaagcgcg agcagcaacc
      661 caacatcctc ggtggtcgtg cccacgggac tgacggtgcc agtcgtcagc ggcgtcccga
      721 tcgaatcgac tcctgcgcgt gaagctccat cgacttcgac cggttccgcg ggcagcccgc
      781 gcggatgggc cgtgcaactg ggtgcgttct cgcaggaagt caatgccgaa gcgctggcgg
      841 gacgcacggc cgccctgctc gcatttctcg aaccggccga cgccttgccg gtgcgcgtgc
      901 cgcgcatcga aaaggatggc gcggtctacc gcgtgctggt gggcgctacc cccgatcgcg
      961 acgcggccct gtcgctggcg cgcgagctcg aacgagtcct tgaccgtccg acgacgctgt
     1021 tgctgcgata gggccaaagc ggcattggcg tgaagaggcc ttactccggc ggcgctttgc
     1081 ggcgccgacc gatcgcctcg tcatagacag tcgatcgcgg cactccggtc atccggctga
     1141 caacggcggc ggcccgcgcg ggggtcattt cctcgagcag cgcgtccagc cagcgctgcc
     1201 cctgcgggtc gagtgccggg cgggcttctt gctccgccgc gtcgatcacg atcacatact
     1261 cgccgcgttc ctcgggctcg agatccgcga gtgccgacgc ggtgtgcacg cggatggttt
     1321 cgaacttctt ggtcagttcg cgggcgatca cgacccgacg gtcaggtgtc aggatgtgtg
     1381 acaagtcccc ctgcagcgcg cggatccggt gcggcgcctc gaaaagcacg gctgccccgt
     1441 cgctcaacgc gatgtcgcgc agaaggcgct cccgctcgcg ggcgcccgca gggaggaagc
     1501 cgatgaagcg gaatgcggtg acccgcaggc cgacagcaga cacggccgcc agtgcactgc
     1561 tggctccggg gatcgggatc acgcgaagat cggcttccag gacggcacgc acgatcaccg
     1621 cgcccggatc ggatacaccc ggggtgccgg catccgacac cagtgccact cgctgtccct
     1681 gttgaagacg gtcgacgatt gcagccgcca cggcccgctc gttgtgctga tgggccgcga
     1741 gcaacggagt ggcaatgccg aaccgggcca gcagcgggcc ggtcacgcgg gtgtcttcgg
     1801 ccgcaatcgc atcgacccgg gcgagagtcc agagcgcgcg cagcgtgatg tcggccgcgt
     1861 ttccgatcgg cagggcaact acatacaatg cccgcacagg aaggtcctgt tcggccagtc
     1921 ccgccagagc ggtcaggtcg tcgtgtcgtg gatggctcgc acccggaccc atgaatcgct
     1981 cgctgctcgt gtcaatgttg ttgcgagggc tgctcctaat ggcagcaagt gtcgcatgcg
     2041 ctgtggacgc ccaggcgccg gcgtcgacag cgatccgatc gaccgttcct gtcgttggcg
     2101 agcgcgcagg gcgcccgacg atcttattgc tgcttccggc ccagtcgacg gccttcgcgc
     2161 tgccggcaga ggcgctgcgt gaaggattct tcgctgccca caaggtgggt ggtgacgata
     2221 tagctatcca ggtcgttgaa ctcgatgaca gtgccgacca gcttggcgtc gcgctcgcct
     2281 ctgcgcgcga ccgtggtgtc ggtatcgtcg tcggcccgct gccccgcacc gcggtgaccg
     2341 atctggtcga ggggcagcgc gcctcgctgc ccctgctcgc gttgaatttt ccggagtcgg
     2401 acggggccgc gccgacgtcg atgctcgcga tggcgctttc ggtcgagtac gaagcgcagc
     2461 gcattgcccg gctcgccctg tcggagttcg tcggcgtccg cacggccgat acgcgccccc
     2521 gcatcgctgt cgtcacacga cccggcggga tcgagcggcg catcgcgcag gcctacgtga
     2581 gtgcgctgcg cagccagggt gaagtgcccc aactggtcga gtggactcct gcgactgctg
     2641 gaaaggtggc ggcgcagctg acgacgtcga cgctcgaagc cgtttttctc gccctgacgg
     2701 cgcgcgacgc cgcgcagatg cgcgcgctga ttccgcgcca gg
//
LOCUS       DATIRT010000102         3647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1192779, whole genome shotgun sequence.
ACCESSION   DATIRT010000102 DATIRT010000000
VERSION     DATIRT010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192779"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VLN25_02490"
     CDS             <1..415
                     /locus_tag="VLN25_02490"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645579.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HSQ09474.1"
                     /translation="RIAAGEKLRLRQRDIVLKGHAIECRINAEDPFKFTPSPGRITGW
                     HPPGGPGVRVDSHAYSGYFVPPNYDSMIGKVICYGDTRDQALARMRIALSEMVVEGIL
                     TNIPLHRELLMDEKFVLGGTSIHYLEKRIAARHSV"
     gene            425..1330
                     /gene="prmA"
                     /locus_tag="VLN25_02495"
     CDS             425..1330
                     /gene="prmA"
                     /locus_tag="VLN25_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HSQ09475.1"
                     /translation="MLEVTLLADEDSAEPLADALLEAGALSVAIEDADAASDLESPLF
                     GEPGFEPERQAWQRSRLRILLEGNQQDSERLIAAAAAAMGRSAELPIESCAAVAEADW
                     VSASQAQFPPTRISERLWIVPSWHDIPDPSAIVVRLDPGVAFGTGTHPTTQLCLAWLD
                     EHVTAGASVLDYGCGSGILAIAAGKLGAGTLVGVDVDEQALAAARANSTVNAVTARYT
                     GPAGLAPGVFDIVVANILSNPLKLLAPALLARVAPGGALVLSGVLERQSDEVIAAYRE
                     ADAAVALRSWRADDGWVCLAGGRSA"
     gene            1402..2538
                     /locus_tag="VLN25_02500"
     CDS             1402..2538
                     /locus_tag="VLN25_02500"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon and DUF3426 domain-containing
                     protein"
                     /protein_id="HSQ09476.1"
                     /translation="MALATKCPQCGAMFRVVADQLKLRGGLVRCGQCRTVFDAIGSLA
                     YVEDAALTASRPMAESTRDPDDNSAAERSGPKTTTATPATAPAERARPAPERRRYPSD
                     RKRKALGPATTLRIAPGLAPVATVSRELPPAPPPAAGRRTTRLTSTEELAATETGVPT
                     LLAPGPLTDAGSDPTRALLDGIEVIEVSPLPEPVAVAAAVLQEEPEFIRAAERAPRRG
                     FSIVFTGGSLLLLLLAVAQLAVSFRSDLLTQWPEARPALSQLCDVFGCTLGWPTQPEQ
                     LAVIGSELQSIAGTDVLELTAVIRNRAAYTQALPALEVTLSDTQNRPVARKIFSPADY
                     LAAAGEPRTRIDEGLAAGADMTIRLHFETRGVQAAGFLVYPFYI"
     gene            2610..3545
                     /locus_tag="VLN25_02505"
     CDS             2610..3545
                     /locus_tag="VLN25_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HSQ09477.1"
                     /translation="MRTLICGSIAYDTVMVFQGRFADHILPDQVHILNVSFLVPEMKR
                     EFGGCAGNIAYSLKCLGGEPLPMATVGEDAGPYLNRLDALAIDRRYVSQVPGSYTAQA
                     FITTDLNDNQLTAFHPGAMGQSHLNAVPGDDAIELGIIAPDGRDGMQQHAQQFAEHGI
                     PFIFDIGQAMPLFNGEELVWFIARATYVTLNDYEARVVVERTRLSLPELAQRVKALVV
                     TRGAEGSEIFLGGTSLRVPVASARRVVDPIGCGDAYRGGLLYGIAKGLDWETTGRLAA
                     VLGAIKIEASGAQNYVADRQALADRFSEAFGYRPW"
BASE COUNT          602 a         1253 c         1175 g          617 t
ORIGIN      
        1 ccgcatcgcc gccggcgaaa aactgcgact gcgccagcgc gacatcgtgc tcaagggtca
       61 cgccatcgag tgccgcatca atgccgagga tccgttcaag ttcacgccgt cgccgggccg
      121 catcaccggc tggcatccgc cgggcggccc cggcgttcgg gtcgattcgc acgcctacag
      181 cggttacttc gtgccgccga actacgactc gatgatcggc aaagtcatct gttacggaga
      241 cacgcgcgac caggcgctgg ctcgcatgcg cattgccctg tccgagatgg tcgtcgaggg
      301 catcctgacc aacatcccgt tgcaccgcga actgctgatg gacgaaaagt tcgtcctcgg
      361 cggcaccagc attcactacc tggaaaagcg catcgctgcg cgacacagtg tgtagtcggg
      421 cgtgctgctc gaagtcacct tgcttgccga cgaagacagt gccgagccgc tggcggatgc
      481 cttgctcgag gcgggcgcac tttcagtcgc gatcgaggat gctgatgcgg catcggacct
      541 cgaatctcca ctcttcggcg agcccggatt cgaacccgaa cgacaggcat ggcaacgcag
      601 ccgactacgc atcctcctcg agggcaatca gcaggacagt gagcggctga tcgcggcggc
      661 ggcggccgcc atggggcgct cggccgaatt gccgattgaa tcctgcgcag cggtagcgga
      721 ggccgactgg gtcagcgcat cgcaggctca gtttccgccc acccgcatca gcgagcgctt
      781 gtggatcgtg ccgtcgtggc acgacattcc cgacccttcg gccatcgtcg ttcgactcga
      841 tccgggcgtc gccttcggaa ccggcacgca cccgacgacg cagttatgcc ttgcgtggct
      901 cgacgagcat gtcaccgccg gtgcaagcgt gctcgactat ggctgcggat ccggcatcct
      961 cgcgatcgct gccggcaagc tgggcgcggg caccctcgtg ggcgtggacg tcgatgagca
     1021 ggcactggcg gcggcgcgcg ccaactcgac ggtcaacgct gttactgcac gctacactgg
     1081 ccctgcaggt ctcgcaccgg gtgtgttcga catcgtcgtg gccaacattc tctcgaatcc
     1141 tttgaagctg cttgcaccgg ctttgctcgc acgcgtggct cccggcgggg cgctggtgct
     1201 gtccggcgta ctcgagcgcc agtcggacga agtgatcgcg gcctatcgcg aggcggacgc
     1261 cgccgtagcc ctgcgctcat ggcgagctga cgacggctgg gtgtgcctcg ccggggggcg
     1321 ctcggcatga ctcgtcgggc ttgtcgtcac ctcaccgtct ggacacttcg gtcgcccgcg
     1381 caccgcggtc gagactgacc gatggcgctc gcaaccaagt gcccccaatg cggcgcgatg
     1441 tttcgcgtgg tcgctgacca gctcaagctg cgcggcggcc tcgttcgttg cggacagtgc
     1501 cgaaccgtgt tcgatgccat cggcagcctg gcctatgtcg aagatgccgc cttgacggcg
     1561 tcacgcccga tggccgagtc gacgcgagat ccggacgaca actcggccgc cgagcgctcc
     1621 ggcccgaaga caacaaccgc cacgccagca accgcgcccg cagagcgcgc tcggcccgca
     1681 cccgaacgcc ggcgctatcc gtcggatcgc aagcgcaagg cgctcggccc cgcaacgacg
     1741 cttcgcatcg cgcctggact ggccccggtc gcaaccgtgt cgcgggaact gccgccagcg
     1801 ccgcctccgg cggctggacg acgaaccacg agactcacct cgacggagga gctggccgca
     1861 acggaaaccg gcgtgcccac gctgctcgca cccggacccc tgaccgatgc cggcagtgac
     1921 ccgacccggg ccctgcttga cggcattgag gtcatcgaag tttcgccgct gcccgagccc
     1981 gtcgcagttg cggccgccgt gctgcaggag gagcccgaat tcatacgcgc agcagagcgc
     2041 gcgcctcggc gcgggttttc gatcgtcttc accggaggct cgctgctgtt gctgttgctt
     2101 gcggtcgcac aactggccgt ctctttccgg tcggatctac tcacgcaatg gccggaggct
     2161 cgcccggcgc tttcgcagct gtgcgatgta ttcggctgca cgctgggctg gccgacgcag
     2221 ccggagcaac tggccgtcat cggtagcgaa ctgcaatcca tcgcgggaac ggacgtgctc
     2281 gaactgacgg ccgtcatacg taatcgtgcc gcctacacgc aggccctgcc ggcgttggag
     2341 gtcacgctga gtgacacgca gaaccgcccg gtggcgcgca agatcttcag tcccgcggac
     2401 tatctcgcgg ccgctggcga gccgcgcacg cgcatcgacg aaggtctggc tgcgggcgcg
     2461 gacatgacga ttcgactcca cttcgagacc cgcggagtgc aggctgcggg cttcctcgtc
     2521 taccccttct atatatagag cacctaacaa gcacgcccgg cgcccgcagg cccgcccgcg
     2581 ggcgcccgac ccactggatt tcgcctcata tgcgcacact catttgcggt tcaatcgcct
     2641 acgacacggt catggtgttc cagggccgat ttgccgacca catccttccg gaccaggtcc
     2701 acatcctgaa cgtctccttt ctggtcccag agatgaagcg cgagttcgga ggttgcgccg
     2761 gcaacattgc ctatagcctc aagtgcctcg gcggcgaacc tctgccgatg gccaccgtcg
     2821 gcgaggacgc gggcccgtac ctcaaccggc tcgacgcact cgcgatcgat cgccgctacg
     2881 tctcgcaggt acccggcagc tatacggcgc aggcgttcat caccaccgat ctcaacgaca
     2941 accagctgac cgcatttcat ccaggggcga tgggccagtc gcacctgaat gccgtgccgg
     3001 gcgacgacgc catcgaactg ggaatcatcg ctccggacgg gcgcgacggc atgcagcagc
     3061 atgcccagca gttcgcggaa cacggcatcc cgttcatctt cgacatcggg caggcgatgc
     3121 cgctcttcaa tggcgaggaa ctggtctggt tcatcgcccg cgctacctat gtgacgctga
     3181 acgactacga ggcccgcgtc gtggtcgagc gcacgcgact gtcactgccg gagctggcgc
     3241 agcgcgtgaa ggcactggtg gtgacgcgcg gcgccgaagg ctcggaaatc ttcctgggtg
     3301 gcacctcgct gcgcgtgccg gttgcgagcg cgcgccgcgt tgtcgatccg atcggctgcg
     3361 gcgatgccta tcgcggcgga ttgctctatg gcattgcgaa gggtctggac tgggaaacca
     3421 cgggccggct tgccgccgtg ctcggcgcga tcaagatcga ggccagtggc gcgcagaact
     3481 atgtggccga tcgccaggcg ctcgcggacc gcttcagcga ggcgttcggc tatcggccct
     3541 ggtgaggccc gccgggcgac gcaacctgcg agcgtctgcc tcggatgcga agcgcttcca
     3601 gttacgagcg ctagatgccg atgcggccga tgatcatctg cagcagg
//
LOCUS       DATIRT010000103        14755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_996155, whole genome shotgun sequence.
ACCESSION   DATIRT010000103 DATIRT010000000
VERSION     DATIRT010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 14755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14755
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996155"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VLN25_02510"
     CDS             complement(<1..341)
                     /locus_tag="VLN25_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSQ09478.1"
                     /translation="MEAKVALITGAGSGVGRAVALAFARTGYTVALAGRRVEALNETA
                     SLAGSDHRVLVLPTDVSDAAAVRSLFDRVAAEAGRLDVLFNNAGSGAPPIPLEDLTAA
                     QWQGVVDVNLTG"
     gene            792..2513
                     /locus_tag="VLN25_02515"
     CDS             792..2513
                     /locus_tag="VLN25_02515"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HSQ09479.1"
                     /translation="MKLLGLKPDVDLEIRAHALLCDYYSERDRDAALAHVAQARALLP
                     RAHRPGLRAHVANCEGEVSEFANDTGRAAALYQEAVTAAEQNGEREMLADALFLRGYL
                     SGLQGAFAAGLADLQRAHGLYAELNLAQQLRNTVNGIASLYSRVGDHQQARDYYEASL
                     QIQMKAGLTRDEVVTRYNLGLVLEKLKDWNAAEQAFNEVQRLSRQIGYARGEAYALRG
                     LAQVQNSRGNHFDALTLLDRAAAQQGATTDKMLRAQILLQRGTALRGLQRPRESIEAL
                     RQALDVFVKADSAGEVATTRAELARALAGLGDWQGAYEQHVQYQTANDALLQRQLDQR
                     YASLTSAKDKENALLQQQKDATVHALNQERRATRLQEAVIVLIVVLAAVLATLAWRGR
                     RTSQAMRTLAMTDELTGVPNRRQVLAQLDQFLRAGNGCSVLIVDIDHFKTINDEHGHL
                     VGDDILRAVSTALRAIANGPVNLGRLGGEEFLIASPEANEIAARRLAERLLAQVRTLD
                     LSHHLPGRRMTVSIGLTVSAPGDTITQLLRRADEALYAAKANGRDCVVARMPSVTPTP
                     TPRITAA"
     gene            complement(2530..3426)
                     /locus_tag="VLN25_02520"
     CDS             complement(2530..3426)
                     /locus_tag="VLN25_02520"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HSQ09480.1"
                     /translation="MQWIGRFVAVALAAGVPCVVMATDRSAELERVVASERAFAARAQ
                     VVNARQAFVEYFAADAIFFAPFAAPAFPRLRESADWGVNIQWRPAAAAISGAGDMGYT
                     TGPAEYRPSADAAPTGFGHYTSVWQRQPDGRFLVVIDVGIDHPAPPARIPDWSAPPQS
                     PAAAPALDLAQRRAALEELRSLDAQIGTAAQAGEISKSFARVLADDARLHLGGRLPAL
                     GRAEVLADLRTEVGAYGWTPEAAAVAESGDFGFVYGRGRWSRARTAETGDLAYLNVWQ
                     RRAGEWRLIVHVSRPVAPRPTQ"
     gene            complement(3568..4908)
                     /locus_tag="VLN25_02525"
     CDS             complement(3568..4908)
                     /locus_tag="VLN25_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSQ09481.1"
                     /translation="MSVHPVKSLILTLPTDWLRRFALAADDLLRDAIYRRLWISIFIS
                     SFGAQVTMLALPLTAAVLLHATPTQMGVLTAMEVLPFVLFSLPSGVYLDRVRKLPVYV
                     WGELLLALFVASVPLAWWAGWLSMTWLYVIGFAIGTVYTIAGSAAQIVLTQIVPRERL
                     VEAHAKNALASSSAEVAGPGLAGVLIKLVGAPFALLADAVLLMVSALILRGIRVTEGP
                     LHVATDFWPALRAGMRFVARHRLLVTMALIVGGWQMCHQAALVVQILFATRTLGLTEG
                     GVGLSYVALGVGTIVTSTVGNRISKRLGPGPTLVLGFVVCGVGWLLGAVAPPSTLGIA
                     MFGLMLMLFGVGAVLIFITFLALRQAVTPPHLLGRMTSTMRWLILIPAGPGALLGGWL
                     GEHRGLRYALAFAGITVLGLAAVAWRQKIIRDTRVLPVVLEDAPLHLPTPGVAS"
     gene            complement(4993..5928)
                     /locus_tag="VLN25_02530"
     CDS             complement(4993..5928)
                     /locus_tag="VLN25_02530"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HSQ09482.1"
                     /translation="MSTFAQQFQIAAPLFALVLIGYLLSRWGRWSSEVSAALSRFVFA
                     VALPALLFRLMSGLAALPPVDPKLLLAYFGGCVLVFILGRGVARFAFRLDGVGQSVFA
                     LGGIFSNNVLLGVPLARSALGEAAMPAVALVLVFNTLLLWTLVTVSVEWARHGELSLK
                     GFRTTARSVLTNPIVASIMAGSIFGLAGGRIPALIDEPLRLLGDAATPLALFVLGMGL
                     SEYGVRQGWREGIAITVLKLVVQPLVVWVLARLLQLSSVETQAVVLLASLAVGVNVYL
                     MSREFRSMEGAVATSMVLSTVLAALTTPLVLTLMG"
     gene            complement(6040..6702)
                     /locus_tag="VLN25_02535"
     CDS             complement(6040..6702)
                     /locus_tag="VLN25_02535"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HSQ09483.1"
                     /translation="MSSLTEPLTVDVVSDIVCPWCYIGKRRLEAALTERGTAATVRWH
                     PFQLNPELPRGGVDRRSYLERKFGGPEGAREVYARVQAAGRDAGVWFEFDRIERQPNT
                     LDAHRLIAWAQRLIPAKTSGLVERLFRAYFTQGLDIGNVEELARLAGQAGYDAYRATA
                     MLTTDEGRAGVVAADQRARAMGVSGVPLFIFNQRRAVSGAQPSAVLLEAMHQSEVAVI
                     TR"
     gene            complement(6804..8030)
                     /locus_tag="VLN25_02540"
     CDS             complement(6804..8030)
                     /locus_tag="VLN25_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HSQ09484.1"
                     /translation="MSRSGWGEWLARGLQALVGVVLGVVLIVGIAGGAHAATGANATA
                     IEAFVREGCPHCAKAEEFLARLQQEQPSLKVVVRDVQKEPAALERLKELARAQGAGAA
                     RVPAVYVGGQLIIGYSEEASTDKLIRSALAGRAASSAAADAGTCEAEDSLACPQTGAN
                     AAAPPESFEVSLFGRTLTLDDVGLPAFTLAMGLLDGFNPCSMWVLLLMISLLAPMNNR
                     RRMLAIAGTFVLIQGIAYFLFMAAWLNLFLVIGLSRASQLIIAAIAIVAGLINVKDFL
                     ALNIGVSLSIPEKAKPGIYSRMRAILHAESLAAAMVGAIILAILVQIVEFLCTSGFPA
                     LFTRILTLRELEPAGYYSYLLLYIAAYMLDDIIILTIGVVMLSRHRLQEREGRVLKLV
                     SGLVMIGLGVYLIFFA"
     gene            8251..9564
                     /locus_tag="VLN25_02545"
     CDS             8251..9564
                     /locus_tag="VLN25_02545"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HSQ09485.1"
                     /translation="MLRLVVLVFMLLLQAAGPALGQSPEPTPQGHILIPAAARPDPAF
                     DVERATRAYLDSVPADARAKSDAYFEGGYWLRLWGFLYGLGVAGVLLFGGISSRLRSW
                     AERRTRRPWLQTMAYGAGLIVVMTLLGLPLAFYQGWYREHEYALSNLSLAAWFLERGK
                     GLLLTVLLGAPVLALIFSVVRKREGNWWLPASVLSVLFMLLVNFIAPVFLAPAFNHFQ
                     PLRPGPVRDAVLSLARANGVTTQHVYWFDASKQTTRISANVSGLGNTMRISLNDNLLG
                     KTSLPEINAVMAHELGHYVLNHPFKLVVQMGLVLAAGFAFTQWAQRRLVARWGSAWGL
                     RGPDDIAGLPALIALLSVWFFLMTPVTNSIVRNAESEADIFGLNAAREPHAFASAAMR
                     LAAYRKLEPSALEEFVFYDHPSGRTRVRMSMTWFAENPSAGAAAR"
     gene            10026..10685
                     /locus_tag="VLN25_02550"
     CDS             10026..10685
                     /locus_tag="VLN25_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09486.1"
                     /translation="MNAYSSWTAAQIGDVATYVAAAAPPSLPPLALPPGTPAPAPTPA
                     PAPAPGTPTTTPTINPSPAMFSSTEVGKYSATTGILVTNSTTVAVSLATPALVPPSAT
                     SSEFVVTTAPAGTTNCVPGFRLDPGTSCSFGVQFAPLAAGARSEKWTINFADGVAARE
                     VTLEGTAIGGTGASGGTASTGSSAANSPADGGGAALGLLHLVGLFALSAVGSLRRRID
                     A"
     gene            10922..11998
                     /gene="ada"
                     /locus_tag="VLN25_02555"
     CDS             10922..11998
                     /gene="ada"
                     /locus_tag="VLN25_02555"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HSQ09487.1"
                     /translation="MTTPLERRAAVTVSDPRWAAVVARDARADGDFVYAVRTTGVYCR
                     PSCGSRAARPENVEFHATSAEAERAGFRACKRCRPSDPARGTVQSALIAELCRYIESA
                     DQPPTLDMLARRAGVSAYHLHRLFKAATGVTPRQYADAHRARRVRNELAARRTVTDAI
                     YGAGFNSSGRFYGQSAALLGMTPTRFRAGGAGTTIRFAVGECSLGSILVAASEQGVCA
                     ILLGDDPETLVQDLQDRFPLARLIGGDAEFEGLVARVVGLVEAPALGLDLPLDVRGTA
                     FQQRVLRSLREIPAGQTASYSEVARRIGAPSSVRAVAHAVASNPLAVAIPCHRVVRTD
                     GALAGYRWGIERKRTLLEREASGA"
     gene            12180..13409
                     /locus_tag="VLN25_02560"
     CDS             12180..13409
                     /locus_tag="VLN25_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HSQ09488.1"
                     /translation="MATTKKKASSSARVRAATSTAKKAVRRPGSSRVKSARRVVVLIA
                     TRKGAWLLHGDAARRSWRTDGPHFLGHIVHHLVLDPRDGRTLLAAAKTGHLGPTIFRS
                     TDFGKTWKEAAKPPAFDKKPDGSGRVVDHTFWLTPGHASEPDVWYAGTSPQGLFRSND
                     GGVSWHSASPINDDPKYIAWMGTVQDGTPDGPKMHSIIVDPRDPKHIYFGMSGGGVHE
                     THDGGKSFSLLIDGLDVVEGFPKNDPTCHDPHNIRMCPSNPDRLYQQNHCGIYRIDRP
                     STTWTRIGRAMPKKVGDVGFTMNVHPRDDNTAWVLPMDGQTVWPRTSPDGQPSVYVTR
                     NGGKTWQRQDAGLPPAQAWWTVKRQAMSLDAHDPVGLYFGTTSGELWASRDEGAKWAC
                     IARNFPEIYALETAEIV"
     gene            13442..13699
                     /locus_tag="VLN25_02565"
     CDS             13442..13699
                     /locus_tag="VLN25_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HSQ09489.1"
                     /translation="MRVLIPSPLRSYTQQSWVDANGATLKEVLADLDRQYPGIRFRMI
                     NEQERMRPHVRFFVRGEPVFDLGLSLAPNDEVLIVQALSGG"
     gene            13828..14265
                     /locus_tag="VLN25_02570"
     CDS             13828..14265
                     /locus_tag="VLN25_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HSQ09490.1"
                     /translation="MRVMVMVKATGDSEAGAMPSEELLTAMGAFNEELVKAGVMLAGE
                     GLHPSAKGKRVRFSGRQRTVIDGPFAETKELVAGFWLWQVKSMEEAIEWVRRCPNPMP
                     TDSEIEIRSVFEAEDFGAEFTPELRAQEDRLRAQADQLARRSK"
     gene            14293..>14755
                     /locus_tag="VLN25_02575"
     CDS             14293..>14755
                     /locus_tag="VLN25_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSQ09491.1"
                     /translation="MATLQRIMPCLWFDNEAEPSANFYVSIFPNSRIKTVAHYGEAGF
                     EHHRRPAGSVMVVIFELDGQELMGLNGGPVFKFSEAISLVVHCDTQDEIDHYWNALGA
                     GGDPRAQQCGWLKDRYGLSWQIVPSIVGQLMTGPNSDRVMAAVMKMKKLDLA"
BASE COUNT         2672 a         4989 c         4815 g         2279 t
ORIGIN      
        1 ccggtcaggt tgacgtcgac cacgccctgc cactgtgccg ccgtcaggtc ctccagcgga
       61 atgggtggcg cgccgctgcc cgcgttgttg aacagcacgt ccaggcgccc ggcctcggcc
      121 gccacccggt cgaaaaggga ccgcactgcc gcggcatcgc tgacatcggt cggcagcacg
      181 agcacgcggt gatctgatcc ggccagcgag gccgtttcgt tcagtgcttc gacccgccgc
      241 cctgccagcg ccactgtata gccggttcga gcgaaggcca gggccacggc ccgtcccacc
      301 ccgctgcccg ctcccgttat caatgcaact ttcgcttcca tacttcacgg ctccctgacg
      361 tttgcatcgg gttgcgagcg ggacctcggt atacggtcca ccgttccgtt cggcggcgac
      421 gacgacggca atcgagctta ccctcggccg tcgctgctga cgcaagcatt gtcgaacccg
      481 ttccaggacc cggacgggtc cccgccctgg catccctgcc acgggtcgga tcgtccgaac
      541 aatgacgcgg atgcgggtga gggcgatcgg acgcgccgga ggccgccttg gcacaatggc
      601 cggccccgct ctctcttttg tcttgccacg tgggtttctc cttccgcctt ggccctgccc
      661 ttgcgtttgc ccttgccgcg ctgataagcg ttccggctgg ggcagcggag gacatagccg
      721 cgctgctcga tcgggcgagc gccgaaacgc ggatcgatcc ggaatccggt cggcgtcacg
      781 ccgaggcggc gatgaagctg ctcgggctga agccggatgt cgacctggag atacgcgcgc
      841 atgcgctgct gtgcgactac tactccgaac gcgatcgcga cgcggctctc gcccacgttg
      901 cccaggcgcg cgccttgctg ccacgcgcac accgtcccgg actgcgcgcc cacgtcgcga
      961 attgcgaagg ggaggtgagc gagttcgcca acgacaccgg acgggccgct gcgctctacc
     1021 aggaagctgt cacggccgcc gaacagaacg gcgaacgcga aatgctggcc gacgccctgt
     1081 ttctgcgtgg ctacctgagc ggcttgcagg gtgccttcgc cgccggtttg gccgacctgc
     1141 agcgcgcgca cggactgtat gcagagctga acctcgcgca gcagctgcgc aacaccgtca
     1201 acggcatcgc cagcctgtac agccgtgtcg gagaccacca gcaggcgcgc gactactacg
     1261 aagcgtcgct gcagatccag atgaaggccg gcctgacccg tgacgaggtc gtgacgcgct
     1321 acaaccttgg gctcgtgctg gaaaagctca aggactggaa tgcggccgaa caggccttca
     1381 acgaagtgca gcgtctgagc cgccagattg gttatgcgcg cggcgaggcg tacgcgctac
     1441 gcgggctcgc gcaggtccag aattcgcgcg gcaatcactt cgatgcgctc acgctgctcg
     1501 accgcgcagc tgcgcagcag ggagccacga ccgacaagat gctgcgcgcc cagatccttc
     1561 tgcagcgggg caccgcattg cgtggactgc aacggccgcg agaaagcatc gaagcactgc
     1621 ggcaggcgct cgacgttttc gtcaaggccg attccgccgg cgaagtggca acgacacggg
     1681 ctgaactcgc ccgcgcgctg gccggcctcg gcgactggca gggcgcctat gagcagcacg
     1741 tgcagtacca gaccgccaac gatgcgctgc tgcagcggca gctcgaccag cgttacgcca
     1801 gcctgacctc agcgaaggac aaggaaaacg cactgctgca gcagcagaag gacgccaccg
     1861 tgcacgcgct gaaccaggaa cgacgcgcga cccgcctgca ggaagccgtg attgtgctga
     1921 tcgtggtgct tgccgcggta ctcgcgaccc tggcgtggcg cggtcgtcgt accagccagg
     1981 ccatgcgcac acttgcgatg actgacgagt tgacaggcgt gccgaaccgc cggcaggtac
     2041 tcgcgcagct ggatcagttc ctgcgcgccg gaaacggctg ctcggtactg atcgtcgaca
     2101 tcgatcactt caagacgatc aacgacgagc acgggcatct ggtcggtgac gacatcctgc
     2161 gcgcggtatc gacggcgttg cgcgccattg ccaacggtcc ggtcaatctc ggccgactgg
     2221 gcggcgagga gttccttatc gccagcccag aggcgaacga gatcgccgcc cgccggctgg
     2281 cagagcgcct gctcgcacag gtccgcacac tcgacctgag ccatcacctg ccggggcgac
     2341 gcatgaccgt cagcatcggg ctgacagtgt ccgcgcccgg cgacaccatc acacagctgc
     2401 tgcgccgcgc cgacgaagca ctgtatgccg ccaaggccaa tggccgcgac tgcgtcgttg
     2461 cgcgcatgcc gagcgtgacg ccgacaccga cacctcgcat caccgccgcc tagtgccagg
     2521 aagtgcgaac tactgcgtcg ggcgcggggc aacggggcgg gacacgtgca cgatcaggcg
     2581 ccactcgccc gcgcgacgct gccagacatt gagataggcc aggtcgcctg tttcggccgt
     2641 tcgcgcccga ctccagcggc cccggccata gacaaaaccg aagtctcccg actcggccac
     2701 tgcggccgcc tccggagtcc aaccgtacgc cccgacctcc gtgcgcaggt cggcaagcac
     2761 ctcggcgcgt cccaatgcgg gcaggcgtcc gccgaggtgc agacgcgcat cgtccgccag
     2821 cacgcgcgca aaggacttcg atatttcgcc cgcctgcgca gccgtgccga tctgcgcgtc
     2881 caggctgcgg agttcttcca gcgcggcacg tcgttgcgcc aggtcgagcg ccggcgctgc
     2941 cgccggtgac tgcggcgggg ccgaccagtc cggtatgcgc gccggtggag ccgggtgatc
     3001 gatgccgaca tcgatgacga ccaggaaacg accgtcgggc tgtcgctgcc acacggacgt
     3061 gtagtgaccg aagccagtgg gcgcggcgtc tgccgaggga cggtattccg cgggtccggt
     3121 cgtatagccc atgtccccgg ctccggaaat cgccgccgcg gcgggacgcc actggatgtt
     3181 gacgccccag tcggcgctct cgcgcaagcg cgggaatgcc ggcgccgcga agggcgcaaa
     3241 gaaaatcgcg tccgctgcaa agtactcgac gaaggcctgt cgagcattga cgacctgggc
     3301 ccgcgcagcg aatgcacgct cgctcgccac gacgcgctcg agttccgccg agcggtcagt
     3361 cgccatcacg acgcaaggca ccccagccgc cagcgcgacg gcgacgaatc gtccgatcca
     3421 ttgcatgccc gtctctccca caggagcgtg cacgctagcg aggttccggg tcgtcggtga
     3481 cgccgtgcga cggacgctcg aagcggctgc acgaatcctg cccatgcgcg atcaatgctc
     3541 ggaccatgcg ccaccgatcg attccggtca gctcgctacg ccgggtgtcg gaagatgcag
     3601 cggcgcatcc tccagcacca cgggcagcac ccgcgtgtca cggatgatct tctggcgcca
     3661 ggcgacggcg gccaacccca gcaccgtgat gccggcaaag gcgagcgcat agcgcagtcc
     3721 ccggtgctcg cccagccagc cgccgagcag cgcgccgggg ccggccggta tcaggatgag
     3781 ccagcgcatc gtgctcgtca tgcggccgag aagatgcggc ggcgtgacgg cctgtcgcaa
     3841 cgcgaggaag gtgatgaaga tcagcacggc tccgacgccg aacagcatca gcatcagacc
     3901 gaacatcgca atgcccagtg tcgacggggg cgcgactgca cccagcagcc agccgacgcc
     3961 gcacacaaca aagcccagca cgagcgtcgg tccgggtcca agacgcttcg agatccgatt
     4021 gccgaccgtg ctggtcacga tcgtgccgac gccgagagcg acgtacgaca gtccgacccc
     4081 gccttcggtc aagcccagcg tgcgcgtcgc aaagagaatc tgcacgacga gcgccgcctg
     4141 gtggcacatc tgccagccgc cgacgatcag cgccattgtc accagcaggc ggtgtctggc
     4201 aacgaagcgc atgccggcac gcaaggcggg ccagaagtcg gtcgcgacat ggagcggtcc
     4261 ttcggtgacg cggatacccc gcagtatcag tgccgagacc atcaacaaca ccgcatcggc
     4321 cagcagcgcg aacggtgcgc ccaccagctt gatcaggaca ccggccagcc cagggccggc
     4381 cacttcggcg gaggagctgg ccagagcgtt cttcgcgtgg gcctcgacga gtcgttcgcg
     4441 cggcacgatc tgcgtaagta cgatctgtgc agcgctgccg gcgatcgtgt agaccgttcc
     4501 gatcgcaaag ccgatgacgt agagccaggt catcgacagc cagccggccc accaagccag
     4561 cggaacgctg gcgacgaaca gcgccagcag gagttcaccc cagacgtaaa ccggcaactt
     4621 gcgcacacga tcgaggtaga cgccggacgg caacgaaaac agcacgaagg gcagcacttc
     4681 cattgcagtc agaacgccca tctgcgtcgg cgtggcgtgc agcagcaccg ccgccgtcag
     4741 cggcagcgcg agcatggtca cctgcgcgcc gaaggaactg atgaagatcg agatccacag
     4801 ccgacggtag atcgcgtcgc gcagcaggtc gtcggcggca agcgcaaagc gccgcagcca
     4861 gtcggttggc agcgtcagaa tgagcgactt cacgggatga acggacatgc cggctcgggg
     4921 ccggcagcgc ggcgcgaagc gtaccggagc cgcgggcaac atgcagcctg cgcctcatga
     4981 cttgcgcccc ctctagccca tcagcgtgag caccaggggt gtggtcaagg cggccagcac
     5041 cgtcgaaagc accatgctcg tggcgaccgc tccttccatc gaacggaatt ctcgcgacat
     5101 caggtagaca ttcacgccta ccgcgagcga ggcaagcagg accacggctt gggtctccac
     5161 cgacgagagt tgcagcagtc gcgcgagaac ccagaccacc agcggctgga cgactagctt
     5221 caacactgtg atggcgatgc cctcgcgcca tccctgccgc actccgtatt cggacaggcc
     5281 catgccgaga acgaagagcg ccagcggtgt tgctgcatca ccgagcaggc gcaggggttc
     5341 gtcgatcagt gcgggaatcc tgccgccggc aagcccgaag atggatcccg ccatgatcga
     5401 tgccacgatc ggattggtca gcacgctgcg cgcggtcgtg cggaagccct tcagcgacag
     5461 ttcgccatgg cgcgcccact cgaccgacac ggtgaccagg gtccagagca gcagcgtgtt
     5521 gaatacgagc acgagagcca cggcaggcat cgccgcctcg ccaagcgcgg atctcgccag
     5581 cggcacgccg agaagcacgt tgttggaaaa gatgccgccg agagcaaaga ccgactgtcc
     5641 gacgccgtcg agccggaaag cgaagcgcgc cacgccgcgg ccgagaatga acaccagcac
     5701 gcaaccgccg aagtaggcca ggagtagctt cgggtcgacg ggtggcagtg ccgccagacc
     5761 gctcatcagc cggaagagca gtgcgggcag cgccaccgcg aatacgaacc tggagagggc
     5821 ggcgctcacc tcgctggacc agcgtcccca gcgggacagc aggtagccga tcagcaccag
     5881 cgcaaagagg ggcgccgcga tctgaaactg ttgcgcgaag gtggacacga catcgggcgc
     5941 agccggtgct gcgcatgggt gaagttgatc agggcatccg caggaactcg tcggcgagcc
     6001 tgggatgctc gcgacgagcc ggtcagtccg acaacgatgt catcgcgtta tcaccgccac
     6061 ttcgctctga tgcatcgcct cgagcagcac cgcggacggc tgcgcgcccg acaccgcacg
     6121 acgctgattg aagatgaaaa gcgggacacc gctgactccc atggcgcgtg cgcgctgatc
     6181 cgcggcgacg actccggcgc ggccctcgtc ggtcgtcagc atggccgtcg cacggtacgc
     6241 gtcatacccg gcctggccgg caaggcgcgc cagttcctcg acattgccga tgtcgagccc
     6301 ctgggtgaag tacgcacgga acagacgttc caccaacccg gaggtcttcg ccggaatcaa
     6361 ccgttgcgcc cacgcgatca gccggtgcgc atccagcgtg ttgggctgcc gctcgatgcg
     6421 atcgaactcg aaccagacgc cggcgtccct gcccgcggcc tgcacgcgcg catagacttc
     6481 gcgcgcgcct tcggggcctc cgaacttccg ctcgaggtag ctgcgccggt caacgccgcc
     6541 gcgggggagc tccggattca gctggaacgg atgccaccgc actgtcgccg ctgtaccgcg
     6601 ctccgtcagc gcggcctcga gccggcgctt gccgatgtag caccaggggc agacgatgtc
     6661 cgacacgaca tcgacagtca gtggctcggt gagagaactc atgacaacgg agcatagcca
     6721 gtcggtgcgc ggattcaagc ggtgatggcc gtctgctacc ggcgccgggc gcgcttgtgt
     6781 gccgacgtac cgcaaccggg ggatcaggca aagaaaatca agtagacacc gaggccgatc
     6841 atcacgaggc cgctgaccag cttcagcacg cgaccttccc tttcctgcaa gcggtggcga
     6901 ctcagcatga ccacaccaat ggtcaggatg atgatgtcgt cgagcatgta ggcggcgata
     6961 tagaggagca ggtaactgta gtaacccgcc ggttcgagtt cccgcagcgt gaggatgcgc
     7021 gtgaacaagg cggggaaacc cgacgtacag agaaactcaa cgatctgcac caggatcgca
     7081 aggatgatcg cgcccaccat cgccgccgcc aggctctcgg cgtgcaggat ggcccgcatg
     7141 cggctgtaga tgccgggctt tgccttctcg ggaatcgaca gcgacacccc gatgttcagc
     7201 gcaaggaagt ccttcacgtt gatcagcccg gcgacgatcg caatggcggc gatgatcagc
     7261 tgcgacgccc gtgacagtcc gatcacgagg aacaggttca gccaggcggc catgaagagg
     7321 aagtaggcga tgccctggat cagcacgaac gtgccggcga tcgccagcat tcggcggcga
     7381 ttgttcatcg gcgccagcaa cgaaatcatc agcagcagca cccacatcga acatgggttg
     7441 aagccgtcca gcaggcccat cgcgagcgtg aaggccggca ggcccacgtc atcgagcgtg
     7501 agggtgcgtc cgaagagcga cacttcgaac gactccggcg gcgcagcggc attcgcgccc
     7561 gtctgcggac acgcgaggct gtcttcggcc tcgcacgtcc cggcatccgc cgccgcagac
     7621 gatgcagcgc gaccggccaa cgcgctgcgg atcagcttgt cggtgctcgc ttcttccgag
     7681 tacccgatga tcagctgccc gccgacgtac acggctggca cacgggcggc gccggcaccc
     7741 tgcgcgcgcg ccagttcctt gagccgctcg agggccgcgg gttccttctg aacgtcacga
     7801 acgacaacct tcagcgacgg ctgctcctgc tgcagtcggg cgagaaactc ctcggccttg
     7861 gcgcaatgcg gacagccctc gcgcacgaag gcctcgatcg ccgttgcgtt cgcaccggtc
     7921 gccgcgtgtg ctccgccggc gatcccgaca atgagcacga cgccaagcac gacacccacc
     7981 agcgcctgca gaccgcgcgc cagccactct ccccaacctg atcgactcac tgtcaccgct
     8041 gtccggccgg gcgcccggca cccgtcaccg ccataacaaa gaacgggccg agtctagaaa
     8101 gcagcgcggt ccgaagcctg cgttacctca cgctgaatgc cgaacgggtt tcatccggat
     8161 caaggccgcg cgagattccg cggttcaagc ggcgcaacgg cggtgagcct ggtccgatta
     8221 ccatgcacgt tttaaggatt ccccctcctc atgctgcggc tagttgtgct tgtgttcatg
     8281 ctgctcttgc aggccgccgg gccggcgctg ggtcagagtc cggaaccgac cccgcagggc
     8341 cacatcctca tccccgccgc ggcgcggccc gatccggcct tcgacgtcga gcgggccacg
     8401 cgggcgtatc tcgacagcgt cccggcggac gcgcgggcga aatcggacgc gtatttcgaa
     8461 ggcgggtact ggctccggct gtgggggttc ctgtacggac taggcgtggc cggtgtgctg
     8521 ctgttcggtg gaatttcgtc gcgccttcgc tcttgggcgg agcgacgaac tcgccgcccg
     8581 tggctgcaga cgatggccta cggcgccggc ctgatcgtcg tgatgacgct gctgggactg
     8641 ccgctggcgt tctatcaggg gtggtaccgc gaacatgaat acgccttgtc caatctttcg
     8701 ctggctgcct ggttcctgga gcgtggcaag ggactgctgc tgaccgtgct gctcggcgcc
     8761 ccggtgctgg ccctgatctt cagcgtcgtg cgcaagcgcg agggcaactg gtggctgcca
     8821 gcctcggtgc tgagcgtgct gttcatgctg ctcgtcaact tcattgcgcc ggttttcctc
     8881 gcaccggctt tcaaccactt ccagccgttg cgtcccggtc cggtgcgcga cgccgtgctc
     8941 tcgctcgcac gtgccaacgg ggtcaccacg cagcacgtct actggttcga tgcgtcgaag
     9001 cagaccacgc gcatcagcgc gaacgtcagc ggactcggca acacgatgcg tatcagcctg
     9061 aacgacaacc tgctgggcaa gacttcactg cccgagatca atgcagtgat ggcgcacgaa
     9121 ctcggacact acgtgctgaa tcatccgttc aagctggtag tgcagatggg gctggtgctg
     9181 gcggccggct tcgcgttcac gcaatgggct cagcgccgtc tggtcgcgcg ctggggttct
     9241 gcgtggggcc tgcgcggccc cgacgatatc gccgggctgc cggccctgat cgcgctcctg
     9301 tcggtgtggt tctttctgat gacgcccgtg accaactcga tcgtgcgcaa tgccgagtcg
     9361 gaggccgaca tattcggact caacgccgcg cgcgagcctc acgcgttcgc gtccgcggcc
     9421 atgcggctgg cggcgtatcg caagctggag ccgtccgccc tcgaagaatt cgtgttttac
     9481 gaccacccga gcgggcgcac acgagtcagg atgtcgatga cgtggtttgc ggaaaatccg
     9541 tccgcgggtg cagcggcccg ctgaaaccgg tcggggacat cgctgcaccc cgctgccggc
     9601 gctgtggctc agaccgtcct cgcccgggca cccgaccggg agccggaccg cgcaaatgga
     9661 gggtggcggg tcatgcgttc gtacggtggg gttacgacgt ttgaccgagg gaaaaaacgg
     9721 cgccgcgacc atagactcgg ccggaacaac ctcctgttgt caggcccgcc atgtcagatc
     9781 gcacatctct gttccacctg cgtgcactac cccccacggt gcttgcgctc gctgctgtcg
     9841 cgatgagcgg ctccgcgcag gcacaaacca acgctaccaa cggcgccacg ctgtacaaac
     9901 agccggtccc tatccaaggt ggagcgccgg cgagttgcga aaactgtcac ggccccgcgt
     9961 ttattttcaa ggccgcgtcg accgcaggtg cgatcagtgc cgccatcagc gccaatcgcg
    10021 gcggaatgaa cgcgtattcc tcatggacag ccgcccagat aggtgacgtt gccacctacg
    10081 tcgccgccgc cgcgcctccg tcgctgccgc cgctggcatt gccgcccggc acgccggctc
    10141 ccgcaccgac gccagccccg gcgccggcac cgggtacacc tacgacgaca ccgacgatca
    10201 atccgagtcc ggcgatgttc agcagcaccg aagtgggcaa gtacagcgcc accaccggca
    10261 tcctggtcac gaacagcacg acggtggccg tttcattggc cacccccgcc ctggtaccgc
    10321 cgtcagccac gtcgagcgag ttcgtcgtga cgaccgcgcc tgccggcact accaactgcg
    10381 taccggggtt caggctcgat ccgggcacga gctgttcgtt cggcgtgcag ttcgcgccgc
    10441 tcgcggccgg tgcgcgcagc gagaagtgga ccatcaactt cgccgacggc gtggcagcgc
    10501 gcgaagtgac cctcgagggc actgccatcg gaggtactgg cgcctcgggc gggaccgcgt
    10561 ccacaggttc cagcgcagcg aactccccgg ctgacggcgg cggcgctgca ctcggactgc
    10621 tgcacctcgt tggcctgttc gctctgtccg cggtcggcag cctgcgccgg cgcatcgacg
    10681 cctgacgctt ccggttgacc cggttccggc ggattccctg ccgggccggg tcccccgtcg
    10741 ttccgaatac aacgagtgcc ggagcacaac gctccagcac tcggctcgtg gacgctcgcc
    10801 aagacgtgtc gtcgccggaa ttctagccgc cctgaattcg atcgcaagcc gcggagttcc
    10861 cgcgcgatcg ccacgcccta gagtccggcc atcgctaaga tgatcggagc ctcgagtgaa
    10921 catgaccacc ccattggagc ggcgcgccgc cgtcacggtc agcgatcccc gctgggcagc
    10981 cgttgtcgcc cgcgacgcgc gcgccgacgg ggacttcgtc tatgccgtta ggacgacggg
    11041 cgtgtactgc cgcccgtcct gcggatcacg cgccgcacga ccggaaaacg tggagttcca
    11101 cgcgacctcg gccgaggcgg agcgcgcggg cttccgcgcc tgcaaacgct gtcgaccgag
    11161 cgacccggcg cgcgggactg tccagtcagc gctgatcgcc gagttgtgcc gttacatcga
    11221 gagtgcggac caacccccga ccctagacat gctggcgcgg cgcgcgggag tgagtgcata
    11281 ccacctgcat cggctgttca aggccgcgac cggggtgaca ccgaggcagt acgccgacgc
    11341 ccatcgagcg cgtcgcgttc ggaacgaact cgcggcacgc cggacggtga ccgacgccat
    11401 ctacggcgcg ggcttcaatt ccagcgggcg gttctatggg cagtccgctg cgctgctggg
    11461 catgacgccg acccgctttc gcgcgggcgg cgcgggaacg accatccgtt tcgccgtcgg
    11521 cgagtgttcg ctcggttcga tcctggtggc ggccagcgag cagggagtct gcgcgatcct
    11581 gctcggagac gatcccgaga ccctggtgca ggatctgcag gatcgtttcc cgcttgcccg
    11641 cctgatcggc ggcgacgctg aatttgaggg actcgtagcg cgggtggtcg gcctggtcga
    11701 agcgccggcg ctcgggctcg acctgccgct tgacgtgcgt ggaaccgctt tccagcagcg
    11761 cgtcttgcgg tccctgcgcg agattcccgc gggccagacg gcgagctact ccgaagtagc
    11821 acggcgcatc ggcgcaccgt cttcggtccg tgcggtagcg catgcggtgg cgtccaatcc
    11881 gctggccgtg gccattccct gccaccgcgt cgtgcgcacc gatggcgccc tcgcgggtta
    11941 ccgctggggc atcgaacgca agcgcacgtt gctcgagcgc gaagcgtccg gcgcctgaac
    12001 cggcgacccg ggcggcgggt cggaaatttt cggatgtcga tttcgtgtcg tcccgttcga
    12061 cgaccttcct gggatcgtgt cgtcacgtgc tcggcagcag gatcggccgc cgggcgctga
    12121 catgacgtcc gttcgcgctt atcgtccctc cgcaaagaac cgacgaccgg gaaggattca
    12181 tggcaacaac gaaaaagaag gcatccagct cagcccgcgt gcgcgcggca acgtccaccg
    12241 cgaagaaagc ggtgcgccgg cccggatcaa gtcgcgtcaa gagtgcgcgc cgtgtcgtgg
    12301 tgctgatcgc aacacgcaaa ggggcatggc tgctccacgg tgatgctgcc cgccgcagct
    12361 ggcgcacgga cggcccgcac ttcctgggtc acatcgtgca tcacctggtg ctggacccgc
    12421 gcgacggacg cacgctgctc gcggccgcga agacgggcca tctgggcccg acgatctttc
    12481 gttcgaccga cttcggcaag acctggaagg aagccgccaa accgccggcc ttcgacaaga
    12541 aacccgatgg cagcggtcgc gtggtcgatc acaccttctg gctcacgccg gggcacgcga
    12601 gcgaacccga tgtctggtac gccgggacat cgccgcaggg gctgttccgt tcgaacgacg
    12661 gcggagtctc gtggcactcg gcatcgccga tcaacgacga tccgaagtac atcgcgtgga
    12721 tgggcacggt gcaggacggc acgcccgacg ggccgaagat gcattcgatc atcgtcgatc
    12781 cgcgcgatcc gaagcacatc tacttcggga tgagtggcgg tggcgtgcac gagacgcacg
    12841 acggaggaaa gtcgttctcg ctgctgatcg acgggctcga cgtggtcgaa ggttttccca
    12901 agaacgaccc gacctgtcac gaccctcaca acatccgcat gtgcccgagc aaccccgatc
    12961 gcctgtacca gcagaaccat tgcgggatct accgtatcga tcgcccgtcg accacctgga
    13021 cccgcatcgg acgcgcgatg ccgaagaaag tcggcgacgt cggcttcacg atgaacgtgc
    13081 atccgcgcga cgacaacacc gcctgggtct tgccgatgga cggacaaacc gtgtggccgc
    13141 ggaccagccc cgacggccag ccctccgtat acgtgacccg caacggcggc aagacctggc
    13201 aacgacagga cgcgggcctc ccgccggcgc aggcctggtg gacagtgaag cggcaggcga
    13261 tgagcctcga tgcccatgat ccggtcgggc tgtacttcgg gacgaccagc ggcgagctgt
    13321 gggccagtcg cgacgaaggg gcgaagtggg cctgcatcgc gcgcaatttt cccgagatct
    13381 acgcgctcga aacggccgag atcgtgtgag tcattgaatg cagcccccgc ggcaacaggc
    13441 gatgagagtg ctgatcccca gcccgctgcg ttcatacacg cagcagtcgt gggtcgatgc
    13501 caatggcgcg actctgaaag aggtactggc cgatctcgac cgccagtatc ccggcatccg
    13561 cttccggatg atcaacgagc aggagcgcat gcgaccgcac gtgcgcttct tcgtgcgggg
    13621 tgaaccggtg ttcgatctgg gcctgtcgct tgcaccgaat gacgaggtgc tgatcgtgca
    13681 ggcactcagt ggaggttgag aagcacggct gaccgctgcg catgcactcc gcgctcctga
    13741 cggcttcaac cgacgtcgaa tcccgggcac cccgttcgac gtagtcctgt taccggcaat
    13801 cgccggtgca gaccgaggag aatcgacatg cgagtgatgg tgatggtgaa ggcgaccggc
    13861 gattccgaag ccggtgcaat gcccagcgag gaactgctga cggccatggg cgccttcaac
    13921 gaggaactgg tcaaagccgg cgtgatgctt gcgggcgagg gcctgcatcc gagtgcgaag
    13981 ggcaagcggg tgcgcttcag tggtcgccag cgcaccgtga tcgacggtcc gtttgccgaa
    14041 acgaaagaac tggtcgccgg attctggttg tggcaggtga agtcgatgga agaagcgatc
    14101 gagtgggtcc ggcgctgccc gaacccgatg ccgaccgact ccgagatcga aatccgctcg
    14161 gtgttcgagg ccgaggattt cggcgccgaa ttcacgccgg aactacgcgc ccaggaagac
    14221 cggctgcgag cgcaggccga ccagctggcc cggcgcagca aatagacgcg ttgaccgcga
    14281 aggagaccga cgatggccac cctgcaacgc atcatgccct gcctgtggtt cgacaacgag
    14341 gccgagccct ccgcaaactt ttatgtctcg atctttccga actcgcgcat caagacggtc
    14401 gcgcattacg gcgaggccgg cttcgagcat catcgtcgac cggccggctc ggtcatggtg
    14461 gtgatcttcg aactcgatgg ccaggagctc atgggcctca atggcggacc ggtgttcaag
    14521 ttctcagagg cgatttcgct cgtcgtccac tgcgacacgc aggacgagat cgaccactac
    14581 tggaatgcgc ttggcgccgg tggcgacccg cgcgcacagc aatgcgggtg gctcaaggac
    14641 cgctacgggc tatcgtggca gatcgtgccg agcatcgtcg ggcagctgat gaccggcccg
    14701 aactcggacc gtgtgatggc ggccgtcatg aaaatgaaaa agctcgatct cgcgg
//
LOCUS       DATIRT010000104         2857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_602811, whole genome shotgun sequence.
ACCESSION   DATIRT010000104 DATIRT010000000
VERSION     DATIRT010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602811"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="VLN25_02580"
     CDS             complement(<1..928)
                     /locus_tag="VLN25_02580"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HSQ09492.1"
                     /translation="MAGPLEGIKVLELGTLIAGPFAARMFAEFGAEVIKIEDPASGDP
                     IRNWRHLHEGTSLWWYVQARNKKSIALNLKDARAQAIARALALDADVVIENYRPGVLE
                     KWGLGYEHLREANPATIMVRISGYGQTGPMRDRPGFGAIGESMGGIRFVSGHPDRPPV
                     RIGISIGDSIAALHGVIGALMALRHRDATGGRGRGVGQFVDVALYEAVFNMMESTVPE
                     FDRYGVVRARTGGALPGIVPSNTYSCANDAAIVVAGNGDAIFKRLMHAIGRPDLAEDP
                     ALARNEGRVKRTKEIDDAIQAWCGERTIDDALS"
     gene            complement(932..1306)
                     /locus_tag="VLN25_02585"
                     /pseudo
     CDS             complement(932..1306)
                     /locus_tag="VLN25_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311152.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
     gene            complement(1355..1957)
                     /gene="recR"
                     /locus_tag="VLN25_02590"
     CDS             complement(1355..1957)
                     /gene="recR"
                     /locus_tag="VLN25_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HSQ09493.1"
                     /translation="MKLGPTLDKLVDALKHLPGIGPRSAQRIAFHLLQHDRESAVRLG
                     ESLLAAVQSIRHCARCNTLTELQICETCASVRRDAQLLCVVESPADQLMVEQSMSFGG
                     LYFVLLGRLSPLDGIGPREIQMEQLLARATDGVVREVIVATSFTPEGDATAHYIGELL
                     RGRNPDLKITRLARGVPAGAELEYTDVNTIAQAMLDRRAA"
     gene            complement(2100..2426)
                     /locus_tag="VLN25_02595"
     CDS             complement(2100..2426)
                     /locus_tag="VLN25_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HSQ09494.1"
                     /translation="MMKGQLAGLMQQAQKMQEGVKRLQEELANTEVEGQSGAGMVKVV
                     MTCKHDVRRVAIDPSLVGEDKDMLEDLVAAAFNDAVRRAEAVSQEKMSSITAGMPLPP
                     GMKLPF"
     gene            complement(2423..>2857)
                     /locus_tag="VLN25_02600"
     CDS             complement(2423..>2857)
                     /locus_tag="VLN25_02600"
                     /inference="COORDINATES: protein motif:HMM:NF023592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau C-terminal
                     domain-containing protein"
                     /protein_id="HSQ09495.1"
                     /translation="LSHAPPAAAPIEFDGDWPTLVERLKVSGLVKELAQRSELVSMQG
                     DHFRLRAPLKTLIEAGTVEKLKAALGAALGRTVRVTADVGSTTGPTAAGVAAKANAER
                     QRQAEESIYADPFVRELIDNFGAAVDPASIRPRDSNDTGTQR"
BASE COUNT          465 a          968 c          912 g          512 t
ORIGIN      
        1 tagacagggc atcgtcgatg gtccgctcgc cgcaccaggc ctggatcgcg tcgtcgattt
       61 cctttgtccg cttgacgcga ccctcgttac gcgcgagcgc cgggtcctcc gcgaggtccg
      121 gccggccgat ggcatgcatc agtcgcttga aaatggcgtc gccgttgccg gcgaccacga
      181 ttgctgcatc gttcgcgcag gaataggtgt tcgagggaac gatgccgggc aacgccccgc
      241 ccgtgcgcgc ccgcacgacg ccatagcggt cgaactccgg caccgtgctc tccatcatgt
      301 tgaacaccgc ttcgtacagc gcgacatcga cgaactgccc cacgccccgc ccccggccgc
      361 cggtcgcgtc gcgatgacgc agcgccatca gcgcgccgat gaccccatgc agggccgcga
      421 tcgagtcgcc gatcgagatg ccgatccgca ccggaggacg gtcaggatgt ccggacacga
      481 agcggatacc ccccatcgat tcgccgattg cgccaaaccc gggtcggtca cgcatcggcc
      541 cggtctgtcc gtagccggag atgcgcacca tgatggtcgc cgggttggct tcacgcaggt
      601 gctcgtagcc caggccccac ttttccagta cgccgggccg gtagttctcg atgacgacat
      661 ccgcgtcgag cgcgagcgct ctcgcaattg cctgcgcgcg ggcgtccttc aggttaagtg
      721 cgatcgattt cttgttgcgc gcctgcacgt accaccagag cgatgtgccc tcatgcagat
      781 ggcgccagtt gcggatgggg tcgccgctgg ctggatcctc gatcttgatc acctcggccc
      841 cgaactccgc gaacatgcgc gcggcgaacg gacccgcgat caaggtgccg agttcgagga
      901 ccttgatgcc ctcgagcggt cccgccatcg ttcaggctcc gagcaggaag atcgtgaggc
      961 aggcgagcca gccgatcagc cacacgagcg atcgcaccgc ctgccagtcc gccagatagc
     1021 agccgaggta ggcaagccgc gccacgatga agatgatcgc caggatgtcg acccgctcct
     1081 gcggcgcgtg ggtcaggtgc gcgacgatga cagcgaccgc gaagaacgga aacgcctaga
     1141 aggcattgag ctgcgcattg ttggcgcgct tgctcaatcc ggtctggcgc tcgagccagg
     1201 cgcgcggatc gcggttgttg tagcgctgcc cgctggtctt cgcgatgatg gtcgccagca
     1261 tcgggagcag cgcggcgacg agaacacacc agaacgcaat cgtcatcgag tccacctttg
     1321 gaacgttagt ttcgaatcag ctggccggcg cttgctatgc cgcgcgtcgg tccagcatcg
     1381 cctgcgcgat cgtgttgaca tcggtgtact cgagttcggc accggccggc acgccgcgcg
     1441 ccaggcgcgt gatcttcagg tccggattcc gtccgcgcag cagttcgccg atgtagtgcg
     1501 cggtggcgtc gccttccgga gtgaacgacg tggccacgat cacctcccgc acgactccgt
     1561 ccgtggcgcg tgccagcaac tgctccatct ggatctcgcg tggaccgatg ccgtcgagcg
     1621 gtgacagcct gccgagcagc acaaagtaga ggccaccgaa ggacatcgac tgctcgacca
     1681 tcaactgatc cgccggggac tcgaccacac acagcagctg cgcgtcacgc cggacgctgg
     1741 cgcaggtctc gcagatctgg agttcggtga gcgtattgca gcgcgcacag tgtcggatcg
     1801 actgcaccgc agccagtagc gactcgccca gtcgcacggc ggactcccgg tcgtgctgca
     1861 gcagatgaaa cgcaatccgc tgcgcggagc gcggtccgat gccgggcaga tgcttcagcg
     1921 catcgacgag cttgtcgagc gtggggccga gtttcatgat gtcgaagcgg gtgtacgcgg
     1981 ccctgcgggc cgcacgtgca cccgcttcgg tagatagtgt gcagcgtgct tcgggcggcc
     2041 gggcgcccgc tgcaaggtca acagcaccag gaaacttggc cgcattgccg cggcgtcacc
     2101 tagaacggca gcttcatgcc gggcggcagt ggcatgccgg cggtgatcga cgacatcttc
     2161 tcctgcgaca cggcttctgc gcggcgcacc gcgtcgttga aggccgcggc gacgagatcc
     2221 tcgagcatgt ccttgtcctc accgaccagg ctggggtcga tcgccacacg gcggacgtcg
     2281 tgcttgcagg tcatcacgac cttcaccatg ccggcgcccg actggccttc gacttccgta
     2341 ttggcgagtt cttcctgcag gcgcttgacg ccctcctgca tcttctgcgc ctgttgcatc
     2401 aagccggcaa gctggccttt catcatcgct gcgttcccgt gtcgttggaa tcgcgcgggc
     2461 gtatcgatgc aggatcgact gccgcgccga agttgtcgat caattctcgc acgaaggggt
     2521 cagcgtagat cgactcctcg gcttgccgct gcctctccgc attggccttt gctgccactc
     2581 ctgccgccgt cggtcccgtc gtgctgccga cgtctgccgt gactcggacc gtgcggccca
     2641 gcgccgctcc gagtgctgcc ttcagctttt ccaccgtgcc cgcttcgatc agcgtcttca
     2701 gcggcgcgcg caggcggaag tgatcgccct gcattgacac gagttcactg cgctgggcca
     2761 gttccttgac gaggccgctg actttgagcc gctcaaccag cgtcggccag tcgccatcga
     2821 actcgatcgg cgcagccgcc ggcggcgcgt gcgaaag
//
LOCUS       DATIRT010000105         3457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1094515, whole genome shotgun sequence.
ACCESSION   DATIRT010000105 DATIRT010000000
VERSION     DATIRT010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094515"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..642
                     /locus_tag="VLN25_02605"
     CDS             97..642
                     /locus_tag="VLN25_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HSQ09496.1"
                     /translation="MNAPTSPLTAAPVHPDEAATGNAAPSMRFMLAHPAHGVALGFGS
                     GLLRPAPGTWGTLAGWGAFIALDAWLSPVAWGVVIFVTFLLGCACAQRTGAALGRPDH
                     GAIVIDEIVAIWIVLLLLPSTLVAQACGFLAFRLFDILKPPPIRALDRRWKNGVGVMV
                     DDLVAAFYALVLAAIVVRVLP"
     gene            821..1345
                     /locus_tag="VLN25_02610"
     CDS             821..1345
                     /locus_tag="VLN25_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HSQ09497.1"
                     /translation="MNSAAERLEHLSAELGRALRAQHLSLTTAESCTAGGVAYAVTLS
                     PGSSQWFDRGFITYTNESKMQVLGVAPAYLRDFGAVSEPVARAMALGALSHSSAQVAV
                     AVTGVAGPGGATPTKPVGTVSFAWAIRRDASQVAWVKTETRHFDGDRGAVRTQSIIAA
                     LETLVGLLKQRQDI"
     gene            1375..2268
                     /locus_tag="VLN25_02615"
     CDS             1375..2268
                     /locus_tag="VLN25_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSQ09498.1"
                     /translation="MKPSELFPDFAPERRHVNGVDICFRQGGSGPALLLLHGFPQTHG
                     IWHRVAPRLAQRFRIVMPDLRGYGDSGKPPSDAEHAPYSKRVMARDMVELMHALGHER
                     YFVCGHDRGGRVAHRLALDHPRDVMRLMVLDISPTRTMYERTTMEFARLYYHWFFLIQ
                     PAPLPETLIGADPGFYLRTKLGAWSSSGTSAFDARALSEYQRCFTTDAIHAMCEDYRA
                     AASIDLEHDRADEASRIGCPVHVLWGERGVVHRLFAPLADWRDKATGAVTGRTLPCGH
                     FIPEEQPDAFVDEAAAFFMGD"
     gene            2268..>3457
                     /locus_tag="VLN25_02620"
     CDS             2268..>3457
                     /locus_tag="VLN25_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09499.1"
                     /translation="MRRGWLVLFLLLSSLAEGARPANELCLSDARGLKVTEARLFDGV
                     GSYRAPPVSDHPEAQRYFEQGLVFGWGFNFAEAARSFRAATVRDPGCAMCRWGIAWAV
                     GPGINHDMDAADMPIARDAIVQARVYAVDARTRELVAAMALRYAGGATHVDDAAARRY
                     AEAMVALAGRRSDDADVAMLAAEALMTAHAYDWWRAEGTAQPWTPQIAALLDLALSLA
                     PDHPGAHHYRIHLYDESRTPAAALVSAQRLPAITPGVGHLVHMPSHVYLRLGRYHEAV
                     LANQAAVSADQRYATATITDRAYAAGYALHNQHFLWASALWSGESQLALSTATAMAAT
                     VTNGPDLQIDVGTREHLVATPWFTLVRFGRWKDILLATVNSNAGPYLRGVAAYARGSA
                     YAHQG"
BASE COUNT          502 a         1192 c         1178 g          585 t
ORIGIN      
        1 catcggggcg atccgggctg gcagcggact gacggtcgtc gatgccgacg gccgggcgat
       61 ggacagggaa gcgcgggcgt tcgatcattt ccgtccatga acgcgcccac gtcaccgctg
      121 acggccgctc ccgtccaccc cgacgaggcg gctaccggca atgccgcgcc gtcgatgcga
      181 ttcatgctcg cgcatccagc gcacggcgtc gcgctcggat tcggcagcgg cctgctgcgc
      241 ccggcgcccg gcacctgggg cacgctggcc gggtgggggg cgttcatcgc actggatgcg
      301 tggctgtctc ccgtggcctg gggcgtcgtg attttcgtca ccttcctgct cggctgtgcc
      361 tgtgcccagc gcaccggtgc cgcgctgggg cgcccggacc atggcgcgat cgtcatcgac
      421 gagatcgttg ccatctggat cgtgctgctg ctgctgcctt ccacgctagt cgcgcaggcg
      481 tgcggctttc tggcgttccg cctgttcgac atcctgaagc cgccgccgat acgggcactc
      541 gaccggcgtt ggaaaaatgg cgtcggcgtc atggtggacg acctcgtcgc cgcgttctac
      601 gccctggtcc tggccgcgat cgtcgtgcgg gtgctgccat gagcgcgcac ggccgctccg
      661 aagcgctcat cccgcagcgc gcagcgcgga gggtttccag acacgcgcac ggccgctccg
      721 aagcgctcaa cccgcagcgc gcagcgcgga gagtttccgg acacgcgcac aaccactccg
      781 aggtacccat cccggagcgc gcagcgcgga gggccgggca gtgaattcgg cggccgaacg
      841 tctcgagcac ctgtccgccg aactcgggcg cgcgctgcgt gcgcagcacc tgtcgctgac
      901 cacggccgag tcctgcacgg cgggaggcgt cgcgtatgcg gtgacgctga gcccgggttc
      961 gagccagtgg ttcgaccgcg gcttcatcac ctacaccaac gaatcgaaga tgcaggtgct
     1021 cggggttgcg ccggcctacc tgcgcgactt cggcgcggtc agtgaacccg ttgcgcgtgc
     1081 gatggcgctg ggcgccctgt cgcacagcag tgcccaggtg gcggttgcgg tgaccggcgt
     1141 ggcaggtccg ggcggagcca cgccgacgaa gccggtcggc acggtcagtt tcgcatgggc
     1201 gatacgtcgc gacgcgagcc aggtcgcctg ggttaagacg gaaacacggc acttcgacgg
     1261 cgatcgcggt gccgtgcgga cgcagagcat catcgcggca ctcgaaaccc tggtcggtct
     1321 gctgaagcag cggcaggaca tctgagcgcc cgccgtcgcg gcgcattgcc ccgtatgaaa
     1381 ccgtcggagc tgttcccgga cttcgcaccc gaacgacgcc acgtcaacgg cgtcgacatc
     1441 tgcttccggc agggcggcag cggtccagcc ctgctcctgc tgcacggttt cccgcagacg
     1501 cacggcatct ggcatcgcgt ggcgccgcga ctggcgcaac gattccgaat cgtcatgccg
     1561 gacctgcgcg gctacggtga ctctggcaag ccgccgagcg atgccgaaca cgctccgtac
     1621 agcaagcgcg tgatggcgcg cgacatggtc gaactgatgc atgccctcgg ccatgaacgc
     1681 tacttcgtct gcggccacga tcgcggcggg cgcgttgcgc accggctcgc actcgaccac
     1741 ccgcgcgacg tcatgcgcct gatggtgctc gacatctcgc cgacgcgcac gatgtacgaa
     1801 cgcacgacga tggagttcgc gcgcctgtac taccactggt tcttcctgat ccagccggcg
     1861 ccgctgcccg agacgctgat cggtgccgac cccgggttct atcttcggac caagctcggc
     1921 gcctggtcgt cgagcgggac gtcggcgttc gatgcccgtg cactgtccga gtaccagcgc
     1981 tgtttcacga cggacgccat ccacgcgatg tgcgaggact accgagccgc cgccagcatc
     2041 gacctcgagc atgaccgcgc cgacgaggcg agccggatcg gatgcccggt gcatgtgttg
     2101 tggggcgaac gcggcgtggt gcaccgtttg ttcgcgccac tggccgactg gcgtgacaag
     2161 gcgaccggcg cagtgaccgg ccgcacattg ccgtgcggtc acttcatccc cgaagagcag
     2221 ccggatgcct tcgtcgacga ggcagccgct ttcttcatgg gcgactgatg cgccgcggct
     2281 ggctggtttt attccttctg ctgtcgtcac tggcggaggg tgcccggccg gccaatgaac
     2341 tctgcctgtc cgacgcgcgt ggcctgaaag tcacggaggc gcgcctcttc gacggtgtcg
     2401 gctcctaccg cgcgccgccc gtttccgatc atcctgaggc gcagcgttac ttcgagcagg
     2461 gactggtgtt tggctggggc ttcaatttcg ccgaggccgc ccgctcgttc cgtgccgcga
     2521 ccgtgcgcga tccgggctgc gcgatgtgtc gctggggcat cgcatgggcc gtgggaccgg
     2581 gtatcaacca cgacatggat gctgccgaca tgccgatcgc ccgcgatgcg atcgtgcagg
     2641 cgcgcgtgta tgcggtggat gcccgcacgc gcgaactcgt ggcggcgatg gccctgcgct
     2701 atgcgggggg cgccacgcac gtcgacgatg ccgctgcgcg ccgctatgcc gaggcgatgg
     2761 tggcgctggc gggacgtcga tcggacgacg ccgacgtcgc catgttggcc gccgaggccc
     2821 tgatgaccgc gcatgcttac gactggtggc gtgccgaggg cacggcccaa ccctggacgc
     2881 cgcagattgc ggcactgctc gaccttgcgc tttcgctcgc tcccgaccat cccggcgcgc
     2941 atcactatcg cattcacctg tacgacgagt cgcgtacgcc cgcggcggct ctcgtgtcgg
     3001 cccagcgcct gccggcgatc acgccgggcg tcggccacct cgtccacatg ccgtcgcatg
     3061 tttatttgcg gctgggtcgg taccacgagg ccgtgctcgc caaccaggca gcggtgtctg
     3121 cggatcagcg ctatgcgacg gccacgatca ctgaccgtgc atacgctgcc ggatacgcat
     3181 tgcataacca gcatttcctc tgggcatcgg ctctgtggag cggcgagtca cagctggcgc
     3241 ttagcaccgc gacggcgatg gcggcgactg tcaccaacgg gcccgacctg caaatcgacg
     3301 tcgggacgcg cgagcacctg gtggcaacac cctggttcac cctggtgcgc ttcggccgct
     3361 ggaaagacat cctcctcgcc acggtgaact ctaatgccgg accgtacctg cgcggcgtgg
     3421 cggcgtatgc ccggggctcg gcctacgcgc atcaggg
//
LOCUS       DATIRT010000106         5756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_504273, whole genome shotgun sequence.
ACCESSION   DATIRT010000106 DATIRT010000000
VERSION     DATIRT010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5756
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504273"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1444)
                     /locus_tag="VLN25_02625"
     CDS             complement(<1..1444)
                     /locus_tag="VLN25_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HSQ09500.1"
                     /translation="MAGLAPAQPGHRDELRADGALRPVWREFFRHLGPGGYGDLPRRR
                     ATIERQVRDDGITYNVYSDDGLRERPWSLDLLPFLISADEWSELEAGLAQRAELMSAI
                     MRDVYGPQQLLAANLLPPALVYGHPGYLRPLVGHRPAGDTYLHILAFDVARAADGQWW
                     VVGNRTQSPSGLGYALQNRLIVSRLFPEAFRRMKVQRLAASYRCLLDTLQRCSPCGTG
                     ESARLVLLTPGPYNETYFEHAYLARYLGIPLVEGSDLTVRDDRTYLKALHGLLPVHGI
                     LRRLDDDFCDPLELRQDSTLGVPGLLQSIRAGKVLVANALGSGFMESPALNGFLPAIA
                     RRELGEKLRLPSLASWWCGERAAFEQVAGELSDKVIKATFPSSQPRPAFEPVIGAAMG
                     ASQLRDVRQLIEADPDAYTVQAYLPLPQAPTWHDGSLVPRAAMVRVFVINDGSGGWHV
                     MPGGLTRIAQREQRVVSMQRGGASQDTWVQT"
     gene            complement(1551..4976)
                     /locus_tag="VLN25_02630"
     CDS             complement(1551..4976)
                     /locus_tag="VLN25_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HSQ09501.1"
                     /translation="MSIHVALNHVTHYRYDRRVTLSPQVVRLRPAPHCRTPVLSYSLR
                     VEPELHFINWQQDPFSNYLARLVFPEKTTEFKVTVDVVAQMAVYNPFDFFLEPGAESF
                     PFVYEANLHHDLQPYLVQEPLTPRFNDFVASVDTHDQRTIDFLVALNQRLQHEIRYLI
                     RLEPGVQTPEQTLSLAAGSCRDTGWLLVQVLRRLGLAARFVSGYLIQLTPDVKALDGP
                     SGTDHDFTDLHAWCEVYLPGAGWIGLDPTSGLLAGEGHLPVACTPEPTSAAPVSGLVE
                     DCEVEFEHTMRIERIYEAPRVTRPYSDAQWRSLDALGQKVDADLQALDVRLTQGGEPT
                     FVSIDDRDGAEWNTSALGPTKRGYATELLLRLKERYGASGFLHFGQGKWYPGEQLPRW
                     ALSAIWRSDGEPCWSDAGLFADERSPSSYTTADADRFMRRLTQRLGIEASYVLPGYED
                     VLYYLWRERRLPANVDPFDSRLDDELERARLRRAFEQGLSSPIGYALPIRREAPRGGG
                     AARWASSQWFLRDERMFLIPGDSPMGYRLPLDSLPWAEPEDQPIAEERDPFAPQAALP
                     LHADIRSEYSPHEVGGGFAEGGSVALQAGFEPPDAAAGGSASVDRAPQRFKSAAGITR
                     STLCVEARDPRRASGPTAEDTGKPAGVLYVFMPPLTALEDYLALLAAVEATAAELGVT
                     IVLEGYPPPRDPRLKVLQITPDPGVIEVNIHPAANWTELVDHTEFLYEAARLSRLSTE
                     KFMLDGRHTGTGGGNHIVMGGATAADSPFLRRPDLLGSLLAYWHNHPSLSYLFSGLFI
                     GPTSQAPRIDEARNDQVYELEIALAELRRRSGAPESAPPWLVDRALRNILIDVTGNTH
                     RAEFCIDKLYSPDGPTGRLGLLEMRAFEMPPHWQMSLAQQLLLRALLARFWRTPYETR
                     LTRWGTELHDRFLLPTFVALDFENVLDELSQAGYPMEPAWFAPHFEFRFPLFGEVAAR
                     GVRLIIRGALEPWHVMGEEGAAGGTVRYVDSSLERVEVKLRGLNDNRHIVTVNGHPIP
                     LNPTGAAGEFVAGVRYRAWQPTSALHPTIGVHAPLTFDVVDAWMGRSLGGCQYHVAHP
                     GGRNYVTFPVNAYEAESRRLARFVDTGHTPGQMVVAAPQPNLEFPFTLDLRRV"
     gene            complement(5074..5756)
                     /locus_tag="VLN25_02635"
                     /pseudo
     CDS             complement(5074..5756)
                     /locus_tag="VLN25_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234287.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase"
BASE COUNT         1011 a         1909 c         1897 g          939 t
ORIGIN      
        1 ccgtctgcac ccaggtgtcc tggctcgcgc cgccacgctg catcgacacg acccgctgct
       61 cgcgctgcgc tatgcgggtg agtcctcccg gcatgacatg ccacccgccg ctaccgtcgt
      121 tgatcacaaa cacccgcacc atggccgcgc gcggcaccag tgaaccgtcg tgccacgtcg
      181 gggcctgcgg cagcggcagg taggcctgca ccgtgtaggc atccggatcg gcttcgatga
      241 gctggcgcac gtcgcgcaat tgcgacgcgc ccatcgcggc gccgatcact ggttcaaatg
      301 ccgggcgtgg ttgcgacgac gggaacgtcg ccttgatgac cttgtcggat agctcgccgg
      361 cgacctgctc gaaggcggca cgctcaccac accaccatga cgcgagcgac ggcaagcgca
      421 gtttctcacc cagttcgcgc cgcgcaattg ccggcaggaa accgttgagc gccggcgatt
      481 ccatgaatcc tgagcccagt gcgttggcga ccagcacctt gccggcccgg atcgactgca
      541 gcaggccggg tacgcccagc gtggagtcct gccttagctc cagcggatcg cagaaatcgt
      601 cgtccaggcg tcgcaggatg ccgtgcacgg gcaacagtcc atggagcgcc ttcaggtagg
      661 tgcggtcatc gcgcacggtg aggtcgctgc cttccaccag agggatgccg aggtagcggg
      721 ccaggtacgc atgctcgaag taggtttcgt tgtaaggccc gggcgtcagc agcacgagac
      781 gtgccgactc accggtgccg catgggctgc agcgctgcag cgtgtcgagc aggcagcgat
      841 agctggccgc cagacgctgc accttcatcc gccggaatgc ctccggaaac agacgcgaga
      901 cgatcagccg gttctgcaat gcatagccca gcccggatgg cgattgcgtg cgattgccga
      961 cgacccacca ttgcccgtcc gcggcgcgtg cgacatcgaa cgcgaggatg tgcaggtacg
     1021 tatcacccgc gggtcgatgt ccgaccagcg ggcgcaggta gccgggatgc ccgtagacca
     1081 gcgccggcgg cagcaggttt gccgccagta attgctgcgg tccgtagacg tcgcgcatga
     1141 tcgccgacat cagctccgcc cgctgcgcca ggcccgcctc gagctctgac cattcgtccg
     1201 cgctgatcag gaagggcagg aggtccagcg accacggacg ctcgcgcagc ccgtcgtcgc
     1261 tgtaaacgtt gtaggtaatg ccgtcgtcgc ggacctggcg ctcgatcgtt gcgcgccggc
     1321 gtggcaagtc tccatagcca cccggtccca gatgccggaa gaattcgcgc cacacgggac
     1381 gcagcgcgcc gtcggcacgc agttcatcgc ggtgccccgg ttgcgcgggc gcaagacccg
     1441 ccacaaggcg cgcggtgtct tgttcaggat ccgaatcgaa caggctttgc gtcacgcctt
     1501 gggcgaactc tgagtcgggt gagtgtcagg tcggcaacga acgcactcga ctacacgcga
     1561 cgaaggtcga gcgtaaacgg aaactcgagg ttcggctgag gcgctgccac caccatctgc
     1621 ccgggcgtat gacccgtatc gacgaagcgc gccaggcgac ggctttcagc ctcgtaggcg
     1681 ttcaccggga aggtgacgta gttgcgaccg ccgggatgcg ccacgtgata ctggcatccg
     1741 ccaagagagc gccccatcca ggcgtcgacg acatcgaacg tcagcggggc atgcacgccg
     1801 atggtcggat gcagtgccga tgtcggctgc caggcgcgat accgcacccc ggcgacgaac
     1861 tcgccggcag caccggtcgg atttaacgga atcggatgtc cgttgaccgt gacgatgtgc
     1921 cgattgtcgt tgagtccacg gagcttgacc tcgacgcgtt cgagcgagga atcgacgtag
     1981 cgcacggtgc cacccgcagc gccctcctcg cccatcacgt gccagggctc gagcgcaccc
     2041 cggatgatca ggcgcacgcc gcgagctgcg acttcaccaa acagcgggaa gcggaactcg
     2101 aaatgcggcg cgaaccacgc cggctccatc gggtagcccg cctgcgacag ttcgtccagc
     2161 acgttttcga aatcgagcgc gacgaaggtc ggcagaagga accggtcgtg cagttcagtg
     2221 ccccagcgcg tcagccgggt ctcgtagggc gtccgccaga agcgcgccag caatgcgcgc
     2281 agcagcagtt gctgcgcaag gctcatctgc cagtgcggcg gcatctcgaa cgctcgcatt
     2341 tccagcaggc caagacgccc ggtgggtccg tcgggcgagt acagcttgtc gatacagaac
     2401 tcggcacggt gcgtattgcc ggtcacatcg atcaggatgt tgcgcaaggc gcgatcgacc
     2461 agccacggcg gggcactctc gggcgcgccg ctgcgccggc gcagttccgc cagcgcgatc
     2521 tccagttcgt acacctggtc attgcgtgcc tcgtcgatgc gcggcgcctg gctggttggc
     2581 ccgatgaaca gtcccgagaa cagatacgac agtgacgggt ggttgtgcca gtacgcgagc
     2641 aggctgccga gcaggtccgg gcggcgcaga aacggtgagt cggccgccgt ggcaccgccc
     2701 atgacgatgt ggttacctcc gccggtgccc gtgtggcgtc cgtccagcat gaacttctcg
     2761 gtcgacagcc gagacagacg cgccgcctcg tacaggaact ccgtatggtc gaccagttca
     2821 gtccagttcg ccgccgggtg aatgttgact tcgatgacac cgggatcggg cgtgatctgc
     2881 agaaccttga ggcgcggatc gcgcggcggc ggatacccct cgagcacgat ggtcacgccc
     2941 agttcggccg ccgttgcctc aaccgcggcc agcaaagcga ggtagtcctc cagtgccgtc
     3001 agcggcggca tgaagacata aagaaccccc gccggtttgc ccgtgtcttc agccgtgggg
     3061 ccgctggcac gccgcggatc acgcgcctcg acacacaagg tggagcgcgt aatgccagcg
     3121 gccgatttga agcgctgcgg cgcccgatcg actgaagcgg aacctcccgc agcggcgtcc
     3181 ggaggttcaa aaccggcctg aagcgccacc gacccgcctt cggcgaatcc gccccccact
     3241 tcgtgcggcg agtattcgga gcggatgtct gcgtgcagcg gcagtgctgc ttgcggtgcg
     3301 aacggatctc gctcctccgc aatcggctgg tcctcaggct ctgcccaggg cagcgaatcg
     3361 agcggcagtc gatagcccat cggcgagtcg cccgggatca ggaacatgcg ctcatcgcgc
     3421 aggaaccact ggctgctggc ccaccgcgcc gcgccgccgc ctcgcggcgc ttcgcgccgg
     3481 atcggcagcg catagccgat cggactcgac agtccctgct cgaatgcacg ccgcagcctc
     3541 gcgcgctcga gttcgtcgtc gagacgcgag tcgaagggat cgacgttcgc aggcaggcgc
     3601 cgctcgcgcc agaggtagta caggacgtct tcgtagccgg gcagaacgta gctcgcttcg
     3661 atcccgagac gctgcgtcag ccggcgcatg aatcggtcgg catccgccgt cgtgtacgac
     3721 gacggcgacc gctcatctgc aaagagccct gcatcgctcc agcacggctc gccatccgac
     3781 cgccagatgg ccgaaagcgc ccagcgcgga agctgttcgc cgggatacca cttgccctgc
     3841 ccgaagtgca ggaaaccgct ggcgccgtag cgttccttca gccgcagcag cagttcggtc
     3901 gcgtagccgc gcttggtcgg acccagcgcg gaggtgttcc actccgcacc gtcgcggtcg
     3961 tcgatcgaaa cgaaggtcgg ttcacccccc tgcgtgagac gcacgtccag cgcctgcagg
     4021 tcggcgtcga ctttctgccc cagcgcatcg agcgaccgcc actgcgcatc gctgtagggg
     4081 cgcgtgaccc gcggcgcttc gtagatgcgc tcaatgcgca tcgtgtgctc gaactcgact
     4141 tcgcagtcct cgaccagccc gctgaccgga gcggcggagg tgggctccgg cgtgcaggcc
     4201 accggcagat ggccttctcc tgcgagcagg ccggacgtcg gatccagtcc gatccagccg
     4261 gcgcctggca gataaacctc gcaccaagcg tgcaggtcgg tgaagtcatg gtcggtcccg
     4321 gagggtccgt cgagcgcctt cacgtccggc gtcaactgga tgaggtagcc cgacacgaag
     4381 cgcgcggcca ggcccagccg gcgcagcacc tgcaccagca gccaaccggt gtcacggcac
     4441 gaaccggcgg cgaggctcag cgtctgctcc ggcgtctgta cgcccggttc gagccggatc
     4501 aagtagcgga tctcgtgctg cagacgctgg ttcagcgcga ccaggaaatc aatcgtgcgc
     4561 tgatcgtgcg tatcgacgct ggcgacaaaa tcgttgaagc gtggcgtcag cggctcctgc
     4621 acgaggtaag gctgcaggtc gtggtgcaga ttcgcttcgt agacaaacgg aaatgactcc
     4681 gcgcccggct cgaggaagaa atcgaacggg ttgtagaccg ccatctgcgc tacaacatcg
     4741 accgtgacct tgaactcggt ggtcttttcg gggaacacca ggcgtgccag gtagttcgag
     4801 aacggatcct gctgccagtt gatgaaatgc agttccggtt cgacccgcag cgagtacgac
     4861 agcaccggcg ttcgacaatg cggcgcggga cgcaggcgca cgacctgcgg cgacagggtt
     4921 acgcggcggt cgtagcgata gtgcgtgacg tgattgagtg cgacgtggat ggacacggca
     4981 gtcccgttca gtggttcgtc gcgccggcgt cgatacggcc ggcgagtctg gagtggcgcg
     5041 cgcgcacggc gggtctcacg ctcgccgcgg ggctcagcgt ggcaccgtgc gaggtccgct
     5101 ctgggcgacc agttgcatgg cgacattggc cgccgagtcg gcttcaaaac tggccggcaa
     5161 cggtgcgcgc ggcggctggg tcggttcggt ctccagcctc ggcgagccgc tgtcgctcgg
     5221 ggcatcgcgc cacgtcggat cggggatctc ctggaacacc tgtttgagtc gcgagcccca
     5281 ggtgttgcgg atcatctgca tgtactggtt gtgatggtcg atctgcacga acttcgtgac
     5341 gcgcagcgca ccttgctcgt agaccagcag gtccagcggc agcccgaccg agatattgga
     5401 gcgcagcgtc gagtccatgg aaatcagcgc gcacttggcc gcctcctcga gtgaagtcga
     5461 cggcgagatc acacggtcga tgatcggctt gccgtacttg gcctcgccga tctggaagta
     5521 cgcgttctcc ggcgtggcct cgatgaagtt gcctgcctag taaagctgaa acaaccgcat
     5581 ccgctcgccg cggatctgcc cgccgaaaat gaagctgcaa ttgaactcca tgcccgcgtc
     5641 cttcagcgcc tggccgtcgc gctggtgcac ttcgcgtatc gcctcaccca ccacgcgcac
     5701 ggcctcgaac agcgtcttag cattccagac actgtcgtcg tgcggaccga cgcgct
//
LOCUS       DATIRT010000107         4787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_996420, whole genome shotgun sequence.
ACCESSION   DATIRT010000107 DATIRT010000000
VERSION     DATIRT010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4787
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996420"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..1357
                     /gene="chrA"
                     /locus_tag="VLN25_02640"
     CDS             29..1357
                     /gene="chrA"
                     /locus_tag="VLN25_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HSQ09502.1"
                     /translation="MNPAGAPAAVSFWQAVAYWLRLGFISFGGPAGQIAIMHRELVEE
                     RRWLSEKRFLHALNYCMLLPGPEAQQLATYIGWLMHRTWGGIVAGALFVLPSLVILIA
                     LSWIYLRFGDVPAVAGILYGIKPAVTAIVLAAAWRIGSRALDHPFLWAIAVAAFVAIF
                     AFNVPFPLIVLVAAVAGWIGARVVPKAFDGTSAHSSAESHGPALIDDDSLRPAHSHWS
                     TAHALRVLLAFLLLWSCAIGVLVALTGWSGTLTQMAWFFTKAALLTFGGAYAVLPYVF
                     QGAVEHYGWLTTAQMMDGLALGETTPGPLIMVVAFVGFVGGWTHQVTGDALFAGGVLA
                     ATVVTFFTFLPSFLFILLGAPFIESTHGQLHFTAPLKGITAAVVGVIVNLAVFFALHV
                     WWPDGRAAGIELASVLISIVALVALMRFKVGVIPVIVASGLTGLALRTFL"
     gene            1366..1707
                     /locus_tag="VLN25_02645"
     CDS             1366..1707
                     /locus_tag="VLN25_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015275857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HSQ09503.1"
                     /translation="MDKITLTELEEWQRARRTFTLLVVRRAKVREADGADIAGTEWRS
                     PDGLFTWKDEILRDRPVVVVCAHGHELSQGSAATLRAMGLDARYLVDGFAGWRDSGRA
                     TVPLNSNEVRT"
     gene            1704..2159
                     /locus_tag="VLN25_02650"
     CDS             1704..2159
                     /locus_tag="VLN25_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HSQ09504.1"
                     /translation="MKWITRERPKIDRVACPWLIARFIDKEPEFLYVPASQVMSEAQI
                     TGAVPYDVPGVEFTHVGELCSFDAFLARYDLTDPALKQLAAIVRGADTSRLELTPQSP
                     GLYALSLGLSHNFSDDHEMLKHGMVMYDALYTWCRACQAETHNWPPKMN"
     gene            2196..2474
                     /locus_tag="VLN25_02655"
     CDS             2196..2474
                     /locus_tag="VLN25_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09505.1"
                     /translation="MNLEPGIVKTVNLDNEQLLMIESPRGDCVCVIHRGIYLIGCSQA
                     QGHDRSGGSSVRTWRTWLAWIRGRLLRHPTPAATAHRNGLRPEQWVPA"
     gene            complement(2490..3545)
                     /locus_tag="VLN25_02660"
     CDS             complement(2490..3545)
                     /locus_tag="VLN25_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019651949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HSQ09506.1"
                     /translation="MTSHSNPWPALRGGLMALLAAVFFGISTPLVQRFGAGVGAFTTA
                     ALLYFGAALVGALSRHGVEREARLARADLPRVLAIAAFGAVIGPVALAWGLQRTSGTS
                     ASLMLTLEAVFTAILAWRLYRESMGSRVWTALLLLVAGGVVLIVDQSRGGQAQLLGLL
                     AVALATAAWGVDNTLSRSLAERDPGQVVLAKALLGSSATAILALVWREPLPSLAAALA
                     LVAVGAAGYGLSLRFYLLGQRAFGAARTGSVFAFAPFIGAALASGLGDRSFTALMLLG
                     SLLMLAGVVLHLAESHAHEHEHESFDHEHAHRHDDGHHAHAHDSMPVGSHSHRHRHEP
                     LRHSHAHVPDAHHPHEH"
     gene            complement(3643..4305)
                     /gene="ureG"
                     /locus_tag="VLN25_02665"
     CDS             complement(3643..4305)
                     /gene="ureG"
                     /locus_tag="VLN25_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HSQ09507.1"
                     /translation="MTTESTAVPSKPSTPARATGPLRIGVGGPVGSGKTTLVEVLCKR
                     LRDRYRLAVITNDIYTKEDERLLVTAEALTPDRIMGVETGGCPHTAIREDASINLEAV
                     DRMCQRFPDLDLIFIESGGDNLAATFSPELSDLTIYVIDVAGGEKIPRKGGPGITKSD
                     LLVINKTDLAPHVGANLDVMRHDTERMRGARPYVMTNLKTGTGLDAVINFIERQGLLV
                     AA"
     gene            complement(4313..>4787)
                     /locus_tag="VLN25_02670"
     CDS             complement(4313..>4787)
                     /locus_tag="VLN25_02670"
                     /inference="COORDINATES: protein motif:HMM:NF013858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HSQ09508.1"
                     /translation="WLRVHDATLAGDEDAVTRWNRELLATRETAELRAESLQMGASLL
                     RVFPALQLGVPPLDPPTYTAAFAIACARLGIARNEGLAAYLWTWSENQVLVAVKTVPL
                     GQQVGQTMLFRLHATVQRAVEIADELTDDELGTAAVGFALQSARHETQYSRLFRS"
BASE COUNT          736 a         1572 c         1652 g          827 t
ORIGIN      
        1 cgctgtacag cggactgcga ggtgctgcat gaaccccgcg ggcgcgccgg ccgctgtcag
       61 cttctggcag gccgtcgcct actggctgcg gctcggcttc attagcttcg gcggcccggc
      121 cgggcagata gcgatcatgc accgcgaact ggtcgaagag cgccgctggc tctcggagaa
      181 gcggttcctg cacgcgctca actactgcat gctgctgccg ggccccgaag ctcagcagct
      241 tgcgacctac atcggctggc tgatgcaccg gacctggggt ggcatcgttg ccggtgcgct
      301 cttcgtgttg ccctcgctcg tgatcctgat cgcgctttca tggatctacc tgcgcttcgg
      361 cgatgtaccg gccgtggccg ggatcctgta cggcatcaag ccggcggtca ccgcgatcgt
      421 gctggctgcg gcctggcgta tcggttcgcg tgcgctcgat catccgtttc tctgggcgat
      481 cgcggtcgcg gcgttcgtcg cgatcttcgc gttcaacgtg ccgtttccgc tgatcgtgct
      541 ggtggctgca gtcgcaggct ggatcggcgc acgcgtcgtt ccgaaggctt tcgacggcac
      601 gagcgctcac tccagcgcgg agtcgcacgg cccggcgctg atcgacgacg actcgctgcg
      661 tcccgcgcac tcgcactggt cgaccgctca cgcgctgcgt gtgctgttgg cgttcctgct
      721 gctgtggtcc tgcgccatcg gtgtgctggt ggcgctcacc ggctggagcg gcacgctgac
      781 gcagatggcc tggttcttca ccaaggcggc gctgctgacc ttcggcggcg catatgccgt
      841 tctgccgtac gtctttcagg gggccgtcga gcactatggc tggttgacga cggcgcagat
      901 gatggacggg ctggcgctcg gcgagaccac gccgggacca ttgatcatgg tcgtggcctt
      961 cgtcggcttc gtcggcggct ggacccacca ggtaacgggg gacgcgctct tcgccggcgg
     1021 agtgctggcg gcgaccgtcg tcacgttttt cacctttctg ccgtcgttcc tgttcatcct
     1081 gctcggcgcg ccgttcatcg agagcacgca cggccagctg cacttcaccg cgccgctgaa
     1141 gggcatcacg gcggcggtgg tcggcgtcat cgtcaacctc gcagtgttct tcgcattaca
     1201 tgtctggtgg cccgacggcc gcgcggccgg catcgagctg gcgtcagtgc tgatctccat
     1261 cgtcgcgctc gtggctttga tgcgtttcaa ggtgggcgtc atcccggtca ttgtggcctc
     1321 cgggctcacc ggtctggcgc tgcgcacatt cctgtaggag atgaaatgga caagatcacg
     1381 ttgactgaac tcgaagagtg gcagcgcgcg cggcgcacct tcacactact cgtcgtgcgc
     1441 cgcgccaagg tccgcgaggc cgacggcgcc gacatcgccg gcaccgaatg gcgctcgccc
     1501 gacggcctgt tcacctggaa ggacgagatc ctgcgcgacc ggccggtggt ggtcgtctgc
     1561 gcgcatggac acgaactgag ccagggctcc gcggccacgc tgcgcgcgat gggactcgac
     1621 gcgcgctacc tggtcgacgg attcgccgga tggcgcgaca gcggccgcgc aacagtgccg
     1681 ctcaattcga acgaggtgcg aacatgaaat ggatcacgcg tgagcggccg aagatcgacc
     1741 gcgtcgcctg cccgtggctg atcgcccgct tcatcgacaa ggagccggag ttcctctacg
     1801 tgccggccag ccaggtgatg agcgaagcgc agatcaccgg cgccgttccc tatgacgtgc
     1861 cgggcgtgga gttcacgcac gtcggcgaac tctgcagctt cgacgctttc ctggcgcgtt
     1921 acgaccttac ggacccggca ctgaagcaac tcgccgcgat cgtgcgcggc gccgacacgt
     1981 cgcggctcga gctgacaccg cagtcgcccg gcctctatgc gctgtcgctc ggcctgtcgc
     2041 acaacttcag cgacgaccac gagatgctga aacacggcat ggtgatgtac gacgcgctgt
     2101 acacctggtg ccgcgcctgc caggcggaga cgcacaactg gccgccaaag atgaactgag
     2161 cggcacctcg atgagtgccg tgaacgggag ccgacatgaa ccttgaaccc ggaatcgtga
     2221 agaccgtcaa tctcgacaat gagcagttgc tgatgatcga gtctccgcgc ggtgattgcg
     2281 tgtgcgtcat ccatcgcggc atctacctga tagggtgcag tcaggcgcaa ggccatgacc
     2341 gctccggcgg ctcgagcgtg cggacgtggc ggacgtggct cgcgtggatc cgcggccggc
     2401 tcctgcgaca cccgactccg gctgcgacag cgcaccggaa cgggctccgg ccggagcagt
     2461 gggtcccggc ctgacaccgg gctaacccgt cagtgctcgt gcgggtggtg cgcgtcgggc
     2521 acgtgcgcat ggctgtgccg cagcggttcg tgccggtgcc ggtggctgtg agagccgacg
     2581 ggcatcgagt cgtgcgcgtg cgcgtggtgc ccgtcgtcgt gccggtgcgc gtgttcgtgg
     2641 tcgaatgact catgctcgtg ctcgtgtgcg tgcgattcgg caaggtgcag cacgacgccg
     2701 gccagcatca gcaggctgcc gagcagcatc agggcggtga acgaacggtc gccgagtccg
     2761 ctcgcgagcg ctgcgccgat gaaaggcgca aaggcgaaca cggagccggt gcgcgcagcc
     2821 ccgaaggcac gctgccccag caggtagaag cgcagactca gcccgtagcc cgcggcgccg
     2881 accgcaacaa gtgcgagcgc cgcggcgagc gatggcaggg gctcgcgcca cacaagggcc
     2941 aggatcgcgg tcgcactgga gccgagcagc gccttggcca gcacgacctg gcccggatcg
     3001 cgctcggcga gcgaacgcga gagcgtgttg tcgacgcccc aggccgcggt ggcgagcgcc
     3061 accgcgagca accccagcaa ttgcgcctgg ccgccgcggc tctggtcgac gatcagcacc
     3121 acgccgccgg cgactagcag cagcaaggcc gtccagacgc ggctccccat ggactcgcgg
     3181 tacagccgcc aggcgagaat ggcagtgaac acggcttcga gcgtcagcat cagcgaggcg
     3241 ctcgtcccgc tggtccgctg cagtccccat gcgagcgcca ccggtccgat cacggcgccg
     3301 aaggcggcga ttgccagcac gcgcgggagg tccgctcgcg ctaggcgcgc ttcgcgctcg
     3361 acaccgtggc gcgacagtgc gccgaccagc gcagcgccga agtacagcag cgccgccgtc
     3421 gtgaacgcgc cgacacctgc gccgaagcgt tgcaccagcg gcgtgctgat gccgaagaag
     3481 acggcggcca gcagtgccat cagcccgccg cgcagtgcgg gccacggatt cgagtgcgag
     3541 gtcatcggcc gggtggcagt tcgtccgact tgaagtcggc aagtggtgcc tgcctctatg
     3601 ctgccacgcg ccttccggtc ccggcgaacc ggtcgtcgcg cgtcaggcag cgaccagcag
     3661 tccctgcctc tcgatgaagt tgatcactgc gtcgaggccg gttccggtct tcaggttggt
     3721 catcacgtag ggccgtgcgc cgcgcatgcg ctcggtgtcg tgacgcatca cgtcgaggtt
     3781 ggcaccgacg tgcggcgcga gatcggtctt gttgatgacc agcagatcgc tcttggtgat
     3841 gccgggtccg cccttgcgcg ggatcttttc gccgccggcg acgtcaatga cgtagatcgt
     3901 cagatccgac agctccgggc tgaaggtcgc cgcgaggttg tctcccccgg actcgatgaa
     3961 gatcaggtcg agatcgggaa agcgctggca catgcggtcg accgcctcga ggttgatcga
     4021 cgcatcttcg cggatcgccg tgtgcgggca gccgccggtt tccacgccca tgatgcgatc
     4081 cggcgtcagc gcttcggcgg tcacgagtag gcgctcgtcc tccttcgtgt agatgtcatt
     4141 ggtgatgacg gccagccgat agcgatcgcg cagacgtttg cacagaactt cgaccagcgt
     4201 cgtcttgcct gagccgaccg gcccaccgac gccgattcgc aatggtccgg tggcgcgcgc
     4261 gggcgtcgat ggcttggaag gcacggcggt ggattctgtt gtcatcagcg gctcaggaac
     4321 ggaacagtcg ggagtactgg gtttcgtggc gtgccgactg cagggcgaag ccgaccgcgg
     4381 cggtacctag ttcgtcatcg gtgagctcgt cggcaatttc cacggcgcgc tgcacggtgg
     4441 catgcaatcg aaagagcatg gtctgtccga cctgctgccc gagtggcacg gtcttcacgg
     4501 cgactagcac ctggttttcg gaccaggtcc acagataggc cgcgaggccc tcgttgcgcg
     4561 cgatgccgag ccgggcgcag gcgatggcga aggccgccgt ataggtcggg gggtcgagag
     4621 gcggcacgcc gagctgcaat gccgggaaga cgcgcagcag cgacgcgccc atttgcagcg
     4681 actcggcgcg cagttccgcg gtttcgcgcg tggccagcag ttcacggttc cagcgagtga
     4741 ccgcatcctc gtcgccggcc agcgttgcgt catggaccct gagccac
//
LOCUS       DATIRT010000108         2327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1487978, whole genome shotgun sequence.
ACCESSION   DATIRT010000108 DATIRT010000000
VERSION     DATIRT010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487978"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..1019
                     /locus_tag="VLN25_02675"
     CDS             120..1019
                     /locus_tag="VLN25_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSQ09509.1"
                     /translation="MKKLLKWTGGLAIVLVVVGIGLVLATNLLFDRKRERVVRVDVKG
                     VTVVSDDATLARGKYLFASRGCGGCHAPNGAGHVFIDDPKGGFRARSPNITPAGVIEK
                     YTEADWVRAIRHGIRPDGRALFIMPSEDYNRFTDADIAALIAFARSLPPVPGGAAEFT
                     VPLPVKALYAIGVVKDAAEKIDHSLPPAVAVAEGLTPEHGRYVANLCLGCHGDKLSGG
                     KIPGGPPSWPAASNLTPGAGTVMTTYDTADKMKAMFRSGKRPDGSAIAVMPFETLREI
                     NDMDVAAMHAYLKSLPPRPAGGR"
     gene            1116..>2327
                     /locus_tag="VLN25_02680"
     CDS             1116..>2327
                     /locus_tag="VLN25_02680"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HSQ09510.1"
                     /translation="MAVSVFDLFKIGIGPSSSHTVGPMRAARLFALRLEHDGVLDRVG
                     RLRVELYGSLGATGKGHGSDKAVLLGLEGEEPATVDVEAIEARLARMRSAQQIRLLGR
                     HALRFVERDDLLFFTRALPFHANGLRFIAHASDDSELAQRTYYSVGGGFVVSDEVAAD
                     GSAQKRIAPDTTILPMPFHSGDELLARCREHGLTIAGVMRANERHWRSDTAIDDGLRQ
                     IWHVMQACVARGLACDGILPGGLKVKRRAAEWHRKLVARDASDPLALLDWVNLWALAV
                     NEENAAGGRVVTAPTNGAAGIIPSVLHYYTRTVPGAGDDGVIDFLLTAGAIGILYKEN
                     ASISGAEVGCQGEVGVACSMAAGALAAVTGGTPEQVENAAEIGMEHHLGLTCDPVGGL
                     VQIPCIERNAIA"
BASE COUNT          398 a          723 c          797 g          409 t
ORIGIN      
        1 tcgagttcac gcctcatcga atctgatgta gacactcgac gcaccgcggg tcggcgagta
       61 ctctccgccg cctgcgactg cccgcgcttt cgggccacgt gacagccatg ggagggagca
      121 tgaagaaact gctcaagtgg accggcggac tagcgatagt tctggttgtc gtcggaatcg
      181 gcctggtgct tgccaccaac ctgctgttcg atcgcaagcg cgagcgcgtg gtccgggtgg
      241 acgtcaaggg cgtgaccgtg gtgagcgatg acgcgacgct ggcgcgcgga aagtatttgt
      301 tcgcatcgcg cggttgtggc ggttgtcatg cgcccaacgg ggcaggacac gtcttcatcg
      361 atgatccgaa aggcggcttc cgggcgcgct cgccgaacat cacaccggcc ggcgttattg
      421 agaagtacac cgaggccgac tgggtgcgag cgatccggca tggaatccgg cccgacgggc
      481 gagcgttatt catcatgccg agcgaggact acaaccgctt cacagacgcc gatatcgccg
      541 cgctgatcgc ctttgcccgc agcctgccac ccgtcccggg cggtgccgcc gagttcaccg
      601 tgccgctgcc ggtcaaggcg ctctacgcga tcggcgtcgt caaggatgcc gctgagaaga
      661 tcgatcacag cttgccaccg gccgtggcgg tcgctgaagg gctgaccccc gagcatggcc
      721 gctatgtggc caatctgtgc ctcggctgtc atggcgacaa gctgtccggc ggcaagattc
      781 ccggcggacc gccgtcttgg ccggccgcct cgaacctcac tccgggagcc ggaacggtga
      841 tgacgaccta cgacacggcc gacaagatga aggccatgtt ccgcagcggc aagcgccctg
      901 acggcagcgc gattgcagtg atgccgttcg aaacgctgcg tgagatcaac gatatggatg
      961 tcgctgcgat gcatgcctac ctgaagtcac ttccaccgcg tccggcgggc ggtcggtagg
     1021 tagggtcgcg tgggcgcatg ccgctgcgtg gacattgacg cgcagcgacg tgcagaccga
     1081 ttcgaagggt gcgatgctgc gccggggaat agcacatggc tgtcagcgtc tttgatctct
     1141 tcaagatcgg catcgggccg tcgagctcgc atacggtcgg accgatgcgc gcagcgcggc
     1201 tgttcgcgct gcgcctcgag cacgatggcg tcctcgatcg cgtcgggcgg ttgcgcgtcg
     1261 agttgtatgg ctccttgggc gcgacgggca agggacacgg cagcgacaag gccgtgctgc
     1321 tcggcctcga aggcgaagag ccggccaccg tcgatgtcga agcgatagag gcgcggctcg
     1381 cgcgcatgcg cagcgcgcag cagattcgcc ttctcggtcg ccatgcgttg cgcttcgtcg
     1441 aacgcgacga cctcctgttt ttcacccgag cgctgccgtt ccatgcgaac ggactgcgct
     1501 tcatcgcgca tgcgtccgat gacagcgaac tcgcccagcg aacctactac tccgtcggcg
     1561 gcggtttcgt tgtcagtgat gaggtggccg ccgacggttc ggcgcagaag cgcatcgcgc
     1621 ccgatacgac gatcctgccg atgccgtttc acagtggcga tgaactgctg gcacgttgcc
     1681 gcgaacacgg gctaacgatt gccggcgtga tgcgtgccaa tgagcggcac tggcgcagcg
     1741 ataccgcgat cgacgacggc ctgcgccaga tctggcacgt catgcaggcc tgtgttgcgc
     1801 gcgggcttgc gtgtgacggt attctgcccg gaggcctgaa ggtgaagcgc cgcgctgcgg
     1861 agtggcatcg aaagctggtt gcgcgcgacg cctccgaccc gctggcactg ctcgactggg
     1921 tcaacctgtg ggcgctcgcg gtcaacgagg aaaacgcggc gggcggtcgc gtcgtcaccg
     1981 cgcccacgaa cggtgctgcc ggcatcattc cgtccgtact gcactactac acgcggacgg
     2041 tacccggtgc cggcgatgat ggcgtgatcg acttcctgct cacggccggc gcgataggaa
     2101 ttctctacaa ggaaaatgca tcaatctccg gcgccgaagt cggctgccag ggcgaagtcg
     2161 gggttgcatg ttcgatggcg gcaggcgcac tcgcggcggt gaccgggggc acgccggagc
     2221 aggtcgagaa tgcggcagag atcggtatgg agcatcacct cgggctgacc tgcgatccgg
     2281 tcggtgggct ggtgcagatt ccgtgcatcg aacgcaacgc gatcgcg
//
LOCUS       DATIRT010000109         2461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1488083, whole genome shotgun sequence.
ACCESSION   DATIRT010000109 DATIRT010000000
VERSION     DATIRT010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1488083"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..1185)
                     /locus_tag="VLN25_02685"
     CDS             complement(229..1185)
                     /locus_tag="VLN25_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSQ09511.1"
                     /translation="MLEATLSQTLNGVSYGLLLFMLAAGLTLIFSMMGVLNFAHASFY
                     MLGAYVAYQLSLWFGFWVALVLAPVLLFIFGSLVERFGLRRVHRGGHVPELLFTFGLS
                     YIIVELVQVIWGRAAVPYRVPVELDGPVAWLQPLGITFPQYRMFMMAIAIGMLAAIWL
                     VLTRTRIGLVIQAALTHPQQVEALGHNVPRVFMLVFGGGSALAGLAGVVGGNAFVTEP
                     AMAGTVGAIVFVVIVVGGMGSLAGAFVAALLIGLLESFGKVSDVSGLRLLSALGLDLA
                     PDAALYPVLKLTMSQVAPVLPYLLLVVILIVRPKGLLGTRED"
     gene            complement(1253..2440)
                     /locus_tag="VLN25_02690"
     CDS             complement(1253..2440)
                     /locus_tag="VLN25_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HSQ09512.1"
                     /translation="MPAQTVKIAYVDPLSGPFAGVGTNLLRHFQFMAEQLNAQQAAGP
                     GVKFEIVPFDNKASPQETLTVLKQVIDQGIRFVVQGQGSGAALALIDAIEKHNERNPG
                     QSMVFLNFAAVDPDLTNSKCSFWHFRFDANTDMKMEALTTFIKDRPEVKNVFLINQNY
                     SHGQQVSRVAKDLLGKKRPDVKIVGDDLHPLGQVRDFSPYIAKMRAANADTVITGNWG
                     PDLALLVRAARDAGLNANFYTYYAGVLGTPTAIGAAGENRVRQVTYWHPNIVPDYPYE
                     KVAQEFKKRFNEEWYAASTYTSLRLLAEGIRGAQSTDPLKVAFAMEGVRLTAPHGEIE
                     MRSSDHQLLQPLWISSWTKLGGPNRNDVEKTGYTWKTEVAYPTYVASQPTSCQMKRPA
                     RPQ"
BASE COUNT          469 a          800 c          792 g          400 t
ORIGIN      
        1 acgatcatga cgcccatctg cgacatcagc gtcagcgcaa agcccttgct gaaaaatgcc
       61 ggcagcagcg cgaacagcag cgcagtgaag ccccagatga tccagcggcc gaagttcagc
      121 ggtcggaagg tgagttcgcg cagatcggtc atgacagctc tgccggccac tcgcagggca
      181 ccgttgacca tcgcggcaca gaagcgtcac tgagtgggcg cgccatcctc agtcctcgcg
      241 cgtgccgagc agccccttgg ggcgcacgat caggatgacg accaaaagca ggtaaggcag
      301 caccggagcc acttgcgaca tcgtgagctt gagcaccgga tagagcgccg cgtcgggcgc
      361 cagatcgagt cccagcgcag agagcagccg caatccggag acatccgaca ccttgccgaa
      421 gctttcgagc aagccgatca gcagcgcggc cacgaacgct ccggccagcg agcccatccc
      481 gcccacgacg atcaccacga agacaattgc cccgacggtg cctgccatcg ccggctcggt
      541 aacgaacgca ttgcctccga ctacgccggc cagcccggcc agcgcggagc cgccgccgaa
      601 gacgagcatg aagacccgcg gcacgttgtg tcccagcgcc tccacctgct gcggatgcgt
      661 cagcgccgcc tgaatcacga gaccgatgcg cgtgcgggtc agcacgagcc agatggctgc
      721 cagcatgccg atcgcgatcg ccatcatgaa catccggtac tggggaaagg tgatgccgag
      781 cggttgcagc caggcaaccg ggccatcgag ttcgaccggg acgcggtagg gaaccgccgc
      841 gcggccccag atcacctgga cgagttcgac aatgatgtac gacagcccga aggtgaacag
      901 cagttccgga acatgacccc cccgatgcac gcgccgcagg ccgaagcgct cgactagtga
      961 gccgaagata aagagcaaca cgggtgccag gaccagcgca acccagaagc cgaaccagag
     1021 gctcagctgg tacgccacat acgcgccgag catgtagaag ctcgcgtgcg cgaagttgag
     1081 cacccccatc atcgagaaga tcagcgtcag gcccgccgcc agcatgaaca gcagcaggcc
     1141 gtagctcacg ccgttgaggg tctggctgag ggtcgcttcg agcactgaac ggaacctgaa
     1201 aaagaaccgg cccggaagtc cgggccggcg acggtgatct gggtttgaaa gcttactggg
     1261 gacgtgcggg gcgcttcatc tggcagctgg tcggctgcga ggcgacatag gtcgggtacg
     1321 ccacctcggt tttccatgtg tagccggtct tctcgacgtc gttgcggttc ggtccgccaa
     1381 gcttggtcca tgacgaaatc cacaacggct gcagcagctg gtggtcgctt gagcgcatct
     1441 cgatctcgcc gtgcggtgcc gtcagccgga ccccttccat cgcaaaggcg accttcagcg
     1501 ggtcggtcga ctgcgcgccc ctgattcctt cagcgagcag ccgcagcgaa gtgtaggtcg
     1561 aggcggcgta ccactcctcg ttgaagcgct tcttgaactc ctgcgccact ttctcgtagg
     1621 ggtagtccgg cacgatgttc gggtgccagt acgtcacctg ccgcacgcgg ttctcgccgg
     1681 cggcgccgat cgcggtcggc gtaccgagca cgccggcata gtaggtgtag aagttcgcgt
     1741 tcagcccggc gtcgcgcgct gcacgcacca gcagcgcgag gtcggggccc cagttgccgg
     1801 tgatgaccgt gtcggcattg gccgcgcgca tcttggcgat gtacggcgag aagtcgcgga
     1861 cctggccaag cgggtgcagg tcgtcgccga cgatcttcac atccggccgc tttttgccga
     1921 gcagatcctt ggcgacccgc gagacctgct ggccgtgcga gtagttctgg ttgatcagga
     1981 agacgttctt cacttccggg cggtccttga tgaaggtcgt cagggcttcc atcttcatgt
     2041 cggtgttcgc gtcgaaccgg aagtgccaga agctgcactt gctgttcgtc aggtccggat
     2101 cgaccgccgc gaagttcagg aacaccatcg actggcccgg attgcgctcg ttgtgcttct
     2161 cgatcgcgtc gatcagcgcc agcgccgcgc ccgagccctg cccctggacg acgaaacgaa
     2221 tgccctggtc gatgacctgc ttgagcacgg tcagcgtctc ttgcggcgag gccttgttgt
     2281 cgaacggcac gatctcgaac ttcacgcccg gaccggcggc ctgctgtgcg ttgagctgct
     2341 ccgccatgaa ctggaagtgg cgcagcaggt tcgtgccgac acccgcgaac ggcccggaca
     2401 gcgggtcgac gtaggcgatc ttgaccgtct gcgccggcac cgtcggagcc gctgctggag
     2461 c
//
LOCUS       DATIRT010000110         6148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1193527, whole genome shotgun sequence.
ACCESSION   DATIRT010000110 DATIRT010000000
VERSION     DATIRT010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6148
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193527"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..302)
                     /gene="rpsP"
                     /locus_tag="VLN25_02695"
     CDS             complement(39..302)
                     /gene="rpsP"
                     /locus_tag="VLN25_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HSQ09513.1"
                     /translation="MVVIRLARGGAKKRPFFNIVATDSRNPRDGRFIERVGFYNPVAA
                     ENEPKLRIAVDRLSYWAGVGARVSPTVARLVKQHAPKQGAAAA"
     gene            607..1065
                     /locus_tag="VLN25_02700"
     CDS             607..1065
                     /locus_tag="VLN25_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09514.1"
                     /translation="MLAPYDGDRIRALGFRMAKFHHKALAALLASLGGALGLNRLYLS
                     QKFWWLPLAVTGMTLPLLIGVRNWYQSPAFFLLMVPVTAGFIQALFIALMPDERFDAA
                     YNAGSSRKNKSGWDAVLVAIATLAGGAIALMATIALLFQTYYERTLGVAQ"
     gene            complement(1081..2172)
                     /locus_tag="VLN25_02705"
     CDS             complement(1081..2172)
                     /locus_tag="VLN25_02705"
                     /inference="COORDINATES: protein motif:HMM:NF033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HSQ09515.1"
                     /translation="MPIDLQPSQARKGRGAVFNVAHRFMSTEREVDGDVLDVGAYDDD
                     PLPPLKTSVALETARSLVTYNASPDIPFDRSINPYRGCEHGCTYCYARPTHSYLNLSP
                     GLDFETKLVAKRNAAELLRRELAVPSYRVAHIALGTATDAYQPIERELRITRAVLEVL
                     SEARHPLGVVTKSALIERDIDLLAPLAADGIAEVFIRVTTLDAQLSRILEPRAASPLR
                     RLRVIETLTRAGIPVHVNVAPVIPFINEPEIERILEAAARAGAVKSHYTVVRLPWEVS
                     PLFEEWLAAHFPDRAQRVLNRVRDMRDGKNYDARFGARMKGTGTWAALVQQRFDKASA
                     KFGLDRKRPDLRTDLFVAPRSAGPQLGLF"
     gene            complement(2321..2908)
                     /locus_tag="VLN25_02710"
     CDS             complement(2321..2908)
                     /locus_tag="VLN25_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HSQ09516.1"
                     /translation="MMAPISRESLLSLEAYARSRKDFRAKVLAHKKNRTVRLGEHVTL
                     IFEDELTVRYQIQEMLRIEKTFEEGGIRDELDAYNPLIPDGTNLKSTMMIEYEDAEER
                     RVELGRLRGIENRVYVQVDGAARVYAIADEDLERENEHKTSSVHFLRFEFEPVMIAAF
                     RQGAAVALGIDHPHYAVRIDEVAPQVQSALAQDFR"
     gene            complement(2945..4297)
                     /locus_tag="VLN25_02715"
     CDS             complement(2945..4297)
                     /locus_tag="VLN25_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HSQ09517.1"
                     /translation="MTVREGSLEAPTRHPLAWKSPEFWDQQALDKELERVFDICHGCR
                     RCVSLCGSFPVLFDLVDNGPTGEVDGVPKEKYKDVVDQCYLCDMCYMTKCPYVPPHPW
                     NLDFPHLMLRAKAVHYKAQGAKATDKFLTSTDKLGMFAGIPIVTQAVNAANKTKPVRA
                     MVESMMGIDRNAWMPSFAETKFRSSAKADAASPVKDGAKTPGKVAIYSTCYINYNEPG
                     IGHDLVAILRHNDIPYVLLDKEACCGMPKLEIGDLDSVETLKNINIPQLARVAREGYA
                     IITPIPSCTLMYKQELPLMFPVDDDVRVVQDSMWDPFEYLVARNKDGLLKTDFKKPLG
                     KVSYHIPCHGRVQNIGRKTEEFLKLTPAIELNTVERCSGHSGLWGTRKEFHAMAMKIG
                     KPVFKQMAETAPDFISSDCQLGAHHIEQGIANFAKDALGGKTPVNAHPLTLVRIAYGL
                     "
     gene            complement(4478..4897)
                     /locus_tag="VLN25_02720"
     CDS             complement(4478..4897)
                     /locus_tag="VLN25_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HSQ09518.1"
                     /translation="MQLKGSKTETNLKDAFAGESQANRRYLYFAAKADVEGYNDVAAV
                     FRSTAEGETGHAHGHLEYLEAVGDPATGLPIGPTRANLKASIAGETHEYTDMYPGMAK
                     SARDEGFDEIADWFETLAKAERSHANKFQRTLDAIVD"
     gene            complement(5077..6148)
                     /locus_tag="VLN25_02725"
                     /pseudo
     CDS             complement(5077..6148)
                     /locus_tag="VLN25_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652454.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
BASE COUNT         1061 a         1947 c         1925 g         1215 t
ORIGIN      
        1 cggcggcggg gttgcagccg cggatccttc tacattcact acgcggcggc tgcgccttgc
       61 ttcggcgcat gctgcttgac caggcgcgcg accgtcggcg acacacgggc gcccacgccg
      121 gcccagtagc tgagccggtc gaccgcgatg cgcagcttgg gctcgttctc cgccgcgacg
      181 gggttgtaga acccgacacg ttcgatgaat cggccgtcgc gcggattgcg cgaatccgtg
      241 gccacgatat tgaagaacgg ccgctttttt gcgccgcccc gggcaagtcg aataacgacc
      301 attgtttctt ccttcagttc tggtgcggca ttaccgacca ccccgtgggc ctcggtcctg
      361 ccaactggac attctgtcct gtgagtcttg ctatcgctcc ggcgtgcagg tcgtaggcgg
      421 gttcacagcg gggcatagcc cgccaccttg cttctggcgc cccgctcgta tggcaacgcg
      481 gaaaacccgt gattgtatct gccggctgcg gcaccaggca agccggaccg gattgcacgg
      541 gttgccaggc gtccacttgg gtccaacaca ccagaccgcg cagacaggga cacatcgagg
      601 gtcacattgc tggctccgta tgatggcgac cgcattcgag cgctcggttt ccgtatggcg
      661 aaatttcatc acaaggccct ggccgcgctg ctggcgtcgc tgggaggcgc gctgggcctc
      721 aaccgactct atctaagcca gaaattctgg tggttaccgc tggccgtcac gggcatgacg
      781 ctgcccctgc tgatcggagt ccgcaactgg tatcagtcgc cggcgttttt cctgctgatg
      841 gttccggtca cggcaggctt catacaggcg cttttcatcg ctctgatgcc ggatgagcgg
      901 ttcgacgctg cctacaacgc gggaagctcg cgcaagaaca agagtggctg ggatgccgtc
      961 ctggtcgcca tcgccacgct ggccggcggt gcaatcgcgc tgatggcgac catcgccctg
     1021 ttgttccaga catactacga acgcacgctc ggcgtggccc agtaggctca aaccgcggcc
     1081 ctagaacaac cccagctgcg gtccggcgga gcgaggagcg acgaacaggt ctgtgcgcag
     1141 gtcgggacgt ttgcggtcga gaccgaattt cgcgctggcc ttgtcgaatc tctgttgcac
     1201 cagcgctgcc caggtgccgg tgcccttcat gcgcgcaccg aagcgggcgt cgtaattctt
     1261 gccgtcacgc atgtcgcgaa cccggttgag gactcgctgt gccctgtcgg gaaaatgcgc
     1321 ggcaagccat tcctcgaaca gtggactgac ttcccacggc aaccgaacca cggtgtagtg
     1381 ggacttcaca gcgccggccc gcgcagcggc ttcgaggatc cgctcgatct cgggttcatt
     1441 gataaacggg atcaccggtg caacgttgac gtgcaccggg atgccggcgc gcgtcagcgt
     1501 ctcgattacc cgcagccggc gcagcgggct cgccgcgcgc ggctcgagga tgcgcgatag
     1561 ctgcgcatcg agcgtcgtga ctctgatgaa cacctcggcg ataccgtcag ccgccagcgg
     1621 agccaagaga tcaatgtcgc gctcgatcag cgcggacttt gtcacgacgc cgagcggatg
     1681 ccgggcctcg ctcagcactt ccagcacggc ccgcgtgatg cgcagttcgc gctcgatcgg
     1741 ctggtaagcg tcggtcgccg tgcccagggc gatgtgggcg accctgtatg acgggaccgc
     1801 gagttcacgc ctcagcagtt cggcggcatt gcgcttggcc acgagcttcg tctcgaagtc
     1861 cagccctggc gacaggttca ggtaactgtg ggtcgggcgt gcgtagcagt acgtgcagcc
     1921 gtgttcacag ccgcggtacg gattgatcga ccgatcgaac gggatgtcgg gcgaggcgtt
     1981 gtaggtgacg aggctgcggg cggtctcgag cgccacgctg gtcttcagcg gtggcagcgg
     2041 atcgtcgtca tacgcgccga cgtcgagcac atcgccatcg acctcgcgct cggttgacat
     2101 gaagcgatgc gcgacattga acaccgcacc gcggcccttt cgcgcctgcg acggctgcag
     2161 atcgatcggg atgcggcgaa aggtgtccgt cgcctccggt agagggtgac gcgaacgcac
     2221 ggctgacggc cctgttagat actgaatttt catacagtat caacacctgt cggtgccgtc
     2281 cagtttccgc tcgctcagcg gatcgacgta cgcgtgcagg tcagcggaag tcctgtgcaa
     2341 gagcgctctg cacctgcggc gcgacctcgt cgatgcgcac tgcgtagtgc ggatggtcga
     2401 ttccgagcgc gaccgcggct ccctggcgga acgccgcgat catcacaggc tcgaattcga
     2461 accgcaaaaa gtgaaccgac gacgtcttgt gctcgttctc gcgttcgaga tcctcgtccg
     2521 caatggcgta cacccgagcg gccccatcga cctggacata gacacgattc tcgatgccac
     2581 gcagccgtcc gagctcgacc ctgcgctcct cggcatcctc gtactcgatc atcatcgtgg
     2641 acttgaggtt cgtgccatcc gggatcagcg gattgtaggc atcgagttcg tcgcgaatac
     2701 ccccttcctc gaacgtcttc tcgatgcgca gcatttcctg gatctggtag cgaacggtga
     2761 gttcgtcctc gaagatcagt gtcacgtgct cgccgagccg gaccgtgcgg ttcttcttgt
     2821 gtgcgagcac cttggcgcgg aagtccttgc ggctgcgcgc atacgcctcg agcgagagca
     2881 ggctttcgcg tgatatcggg gccatcatca tctccatgtc cttccgggct gcgatccgtt
     2941 caattcagag tccgtaggcg atgcgcacca gcgtcagcgg atgcgcattg accggtgtct
     3001 tgccaccgag ggcatccttg gcgaaattcg cgatgccctg ttcgatgtgg tgagcaccga
     3061 gctggcagtc cgagctgatg aagtcgggcg cggtctctgc catctgcttg aataccggct
     3121 tgccgatctt catcgccatc gcgtggaatt ccttgcgcgt gccccacagg cccgaatggc
     3181 ccgagcagcg ttccactgtg ttcagctcga tggccggagt gagcttgagg aattcttcag
     3241 tcttgcggcc gatgttctga acgcggccgt ggcaggggat gtgatacgac accttcccga
     3301 gcggcttctt gaaatccgtc ttcagcagcc cgtccttgtt gcgcgcgacg aggtactcga
     3361 acggatccca catcgaatcc tgaaccactc tcacatcgtc atccaccggg aacatcagcg
     3421 gcagttcctg cttgtacatg agcgtgcatg acgggatcgg cgtgatgatc gcgtagccct
     3481 cacgcgccac ccgggcgagt tgggggatgt tgatgttttt cagcgtctcg acggaatcca
     3541 ggtcgccgat ctcgagcttt ggcatgccgc agcacgcttc cttgtcgagg agcacgtacg
     3601 gaatgtcgtt gtgccgcagg atcgccacca ggtcgtggcc gattccgggc tcgttgtagt
     3661 tgatgtagca ggttgagtag atcgccacct tgcccggcgt cttggctccg tccttgaccg
     3721 gagacgcagc atccgccttc gccgacgagc ggaacttcgt ttccgcgaag cttggcatcc
     3781 atgcattgcg gtcgatgccc atcatcgatt cgaccatcgc gcgcaccggc ttcgtcttgt
     3841 tcgccgcgtt cacagcctga gtgacgatcg gaatgccggc gaacattccg agcttgtcgg
     3901 tggaagtaag gaatttgtcc gtggctttgg cgccctgtgc tttgtagtgc actgccttgg
     3961 cgcgcagcat caggtgcggg aagtcgagat tccaggggtg cggcggtacg taagggcact
     4021 tggtcatgta gcacatgtcg cacaggtagc actggtcaac aacgtccttg tacttttcct
     4081 tgggcacgcc atcgacttcg ccggtcggac cgttgtcgac caggtcgaac aggaccggga
     4141 acgatccgca caagctgacg cagcgtcggc atccgtgaca gatgtcgaac acacgctcaa
     4201 gttccttgtc gagcgcctgt tggtcccaga actcggggct cttccacgcc agtggatggc
     4261 gggtgggagc ctcgaggctg ccctcgcgta cggtcatagg attctcggaa agggtgatct
     4321 gatggtcatg cgaggctcag ccaagcggcg ctcgcactgc ttggcttaac cggccgcgct
     4381 gccacggccc actttctcgc gtcgagccgg cgggtcaatt ccgccggctc ccacgcgaac
     4441 tgtctctccc gcgccaaggc gccgggaaca gcaggaccta gtcgacgatc gcgtccagcg
     4501 tccgctggaa cttgttggcg tgcgaacgct cggccttcgc cagcgtctcg aaccagtcgg
     4561 cgatctcgtc gaagccctcg tcgcgggctg atttcgccat tcccgggtac atgtcggtgt
     4621 actcgtgcgt ttcgccggcg atcgacgcct tgaggttggc cctggtcggg ccgatcggaa
     4681 gacccgtggc cggatcaccg accgcctcga gatactcgag gtggccatgc gcatggccgg
     4741 tctccccttc cgcggtcgaa cggaagacgg cggccacatc gttgtaaccc tcgacgtcgg
     4801 ccttggctgc gaagtaaagg tatcgacggt tggcctgcga ttcgccggca aacgcgtcct
     4861 tcaggttcgt ttcggttttc gagcccttca gttgcatgaa accctccttg ctggattcga
     4921 caagaacact gcgtgcgacc actccgcagg ccgacggctg aaaggagcct acgccgccga
     4981 aaccggtagg actaattaat tgtcgatatt acttctatag tccttgacta tagacggctt
     5041 ggcaccggac caacgatggc cacggacacc tggatgtcag aactgttctt cggtgagagc
     5101 cagagtgccc gcactgccgg aaacgatgct ggcagacagc cccgggacag tcaccagcag
     5161 gtgatcggcg aagaagcgcg cagtcgcaat cttggcgttt gcaaactgca cgtcgtcgcc
     5221 cgtggcgatc aggcgccgcg ccgcaagagc ggcgcgcgcc agttgccaac cgccgtgcgc
     5281 aatgcccgcc agcttcagat aaggcaccga tccggcgaaa acggcgcgaa tgtcgctccg
     5341 aaagtgcgcg acgacgaatt cgaccaccgc ttcataggag tcgacagcca cggccagccg
     5401 ttgaccgatg gccgcgcaat cgtctcccgc ccgtgcgagc tcggccgcgg ttgcgcgcat
     5461 ctgggcgatg aacgtgcggg ccgtcgcccc gccgtcacgc gacgtcttgc ggctgacaag
     5521 atcgttggcc tggatcgccg tggtgccctc atagatcgtc aggatgcgag cgtcgcggta
     5581 gaactgcgcg gcaccggttt cctcgatgaa acccatgccg ccgtgtacct gcacgccggt
     5641 cgacgcgacg tcgatcgaga tctccgtgga ccagcccttg acgatcggca cgaggtactc
     5701 gtaggccgac tgtgcttcct tgcggaccgc ggcatcggga tcgtgatggg ccacatcgtg
     5761 gtagccagcc gccacgtagg cgacagcgcg cgcggcttcg gtgctggaac gcatgtacat
     5821 cagcatgcga cgcacgtccg gatgccggat gatcgcgacc ggcccgctgg aaccttcgac
     5881 agcgcggctc tgcacgcgat ccttcgcata cgaggccgcc ttctggtagg cgcgatcgca
     5941 gaccgcgatg ccctgcatgc caacctggaa gcgagccgcg ttcatcatca cgaacatgta
     6001 ctcgagcccg cggttttcct cgccgatcag gtagccaatc gcgcccgggc cgacctaacc
     6061 cttgccgtcg ccgaacagca gtacacaggt cgggctcgcc ttgatgccga gcttgtgctc
     6121 gatcgaggcg cagtagacat cgttgcgt
//
LOCUS       DATIRT010000111         5579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1586690, whole genome shotgun sequence.
ACCESSION   DATIRT010000111 DATIRT010000000
VERSION     DATIRT010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586690"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(277..1392)
                     /gene="ald"
                     /locus_tag="VLN25_02730"
     CDS             complement(277..1392)
                     /gene="ald"
                     /locus_tag="VLN25_02730"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HSQ09519.1"
                     /translation="MLIGVPKEIKVHEYRVGMTPSSVREAVAHGHQLIVETHAGAGIG
                     AADDEYRKAGADVVGSAAEVFKRADMVVKVKEPQAIERKQLREGQVLFTYLHLAPDPE
                     QARDLIASGAICIAYETVTSPSGGLPLLAPMSEVAGRLAVQAGARCLEKSAGGMGMLL
                     GGVPGVAPGRIVILGAGVVGTNSAQMAVGTGAQVVVIEKSTDALRRIDAMFNGRVITV
                     FSNQDNVEREVLRADLVISGVLIPGAEAPKLITKAMLPKMKAGSVIVDVAIDQGGSVE
                     TSRPTTHAEPTYVVDGVVHYMVANMPGAVPRTSTYALNNATLPFTLALADKGWHKALH
                     DDPHLKAGLNIAAGKVTYKAVAKALSLGYTAADAVIA"
     gene            complement(1466..2677)
                     /locus_tag="VLN25_02735"
     CDS             complement(1466..2677)
                     /locus_tag="VLN25_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HSQ09520.1"
                     /translation="MKPESAHLWKAPAWALSLLLACLGMLGPFAIDTYLPAFATIRTS
                     LDATTLQMQQTLSAYLFGFAAMNLFHGALADAYGRRPVVLWGVAAFTVASVGCALSQT
                     IGQLVFFRAMQGLATGAGIVVSRAIIRDMFPPAQAQRVMSQVTIFFGVAPAVAPLIGG
                     LLYVNIGWRSIFWLLAGVGLALWLANWRVLPETLHKRDVQPFNVSNLFRGYAQLLGHR
                     NFMLLALASGIPFNGMFLYVLSAPAFLGELLGLAPTQFFWFFCMTIGGIMGGAWLSGQ
                     LAGRIRPRKQIRYGFTVMVVVSIVNVLANALFTAQAWWALPLIGLFAFGWSLMVPVVT
                     IMVLDLVPERRGLASSLQAVVGSVANALVAGALAPAVMHSTLGLAVASFGMMCIGLVA
                     WQIVRRKLQSH"
     gene            complement(2757..5279)
                     /locus_tag="VLN25_02740"
     CDS             complement(2757..5279)
                     /locus_tag="VLN25_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSQ09521.1"
                     /translation="MTTQVRPPQAARIVVIGGGIMGCSVAYHLSSFGETDVVLLEQGR
                     LTSGTTWHAAGLVGQLRTHESMTRLIRYSTDLYARLEGETGLSTGWKQCGSLAVARTA
                     DRMIQLKRTAAVAQAYGVSCDILSPSEAGALYPIMETGDLQGAVWLPGDGKANPADLT
                     LALAKGARNRGVRICEGVRVTGDATQTGSGKRISHLEWISKDGEAGRIAADIVVLCGG
                     QWSRALGRELGVAVPLFSCEHYYIVTDVVPGVHRDLPVMRDPDGYIYFKEEVGGLLMG
                     GFEPEATPWLQSARHRGGIPENFEFQLLPDNWDAFQILLDNAMIRVPSLENAQVKQFY
                     NGPESFTADNNFIVGPAPGIDNLYVGCGFNSMGIASAGGAGKALAEWIMQGEPAMDLW
                     PVDSRRFASFNANETWLPDRIAEVLGMHYKMPWPNRELESARPFRRSPLYAQLRDAGA
                     CFGSKMGWERANFFAPSPAQAQIEYGWGHQNWHPWVAAEHRACREAVAVFDMSSFAKL
                     LVQGRDAHTVLQWLVANDVPLEPGRTIYTGMLNERGGYESDFTITCLGQDEYLIVTGS
                     AQAVRDRDVIRRAIAALPAPANVQVTDITPMLAMLAVMGPRSRELLQRVSTADFSNAA
                     FPFGTSQEIDLGYATVRATRLTYVGELGWELYTPVEFAVGVHERLMSAGRDLGLALGP
                     MNAGYYAIDSLRLEKGYRAWGRELTPDINPFEAGLAFAVSLEKPAEFRGRRALQALRE
                     AGGAAAVARRIVSLVVDAPHTNLWSGELILRNDVPVGSVTSAAFGHTICKPVALGIVS
                     NAQGPAERAWIESGIFTVDLAGERYPAQVALKAPLDSTATKA"
BASE COUNT          980 a         1874 c         1813 g          912 t
ORIGIN      
        1 acgaggaaca cgaacggcag tgccacgagt cctgcgccgg cgacgatcgc cgcggtgtcg
       61 agcagcacat cgctgccgcc cttgatcatc gcgttcctgc tggccgcatt gctgcacgcc
      121 agctggacgg caaaggaaac ctcaagactc acggtgtggt cggcgcggca ggcctccgtg
      181 gatccatccg caaccgtttg cgccgagacg accgaaaacg ccggaatacc gaggcggcgc
      241 gacgcaaccg ggtactgggg gagcgctcgt acagactcaa gcgatcacgg catccgccgc
      301 cgtgtagccg agactcaagg cctttgcgac cgccttgtac gtaaccttgc cggcggcaat
      361 gttgagtccg gccttcagat gtggatcgtc gtgcagcgcc ttgtgccagc ccttgtccgc
      421 gagcgccagc gtaaagggca gcgtggcatt gttaagggca tacgtggacg tgcgggggac
      481 ggcccccggc atgttcgcca ccatgtagtg gacgacgccg tcgacgacat aggtcggttc
      541 ggcatgtgtt gtcggtcgac tggtctcgac gctgccgccc tggtcgatcg cgacgtcgac
      601 gatcacggag ccggccttca tcttcggcaa catcgccttc gtgatcagct tgggcgcttc
      661 ggcaccggga atcagcaccc cggagatcac gaggtcggcg cgcagcacct cgcgctcgac
      721 attgtcctgg ttcgagaaca cggtgatcac gcggccgttg aacatcgcgt cgatccgccg
      781 cagtgcatcg gtcgactttt cgatcaccac tacctgcgcg ccggtgccga cggccatctg
      841 cgcggagttc gtgccgacaa caccggcgcc gaggatgacg atgcgtcccg gcgccacgcc
      901 cgggacgccg ccaagcagca tgcccatgcc gccggccgac ttttctaggc agcgtgcgcc
      961 ggcctgcacc gcgagccgcc ctgccacttc gctcatcgga gcgagcagcg gcaggccgcc
     1021 ggagggtgaa gtgaccgttt cgtaggcaat acagatcgcc ccactggcga tcaggtcccg
     1081 ggcctgttcg ggatccggcg ccaggtgcag gtaagtgaag agcacctggc cttcgcgcag
     1141 ctgtttgcgt tcgatcgcct gcggttcctt gaccttgacc accatgtcgg cgcgcttgaa
     1201 gacctcggcc gcgctgccga cgacatcggc gccggccttg cgatactcgt catcggcggc
     1261 gccgatgccc gcaccggcgt gagtctcgac gatcaactgg tgtccgtgcg cgacggcctc
     1321 ccgcaccgac gatggcgtca tgccgactcg gtactcgtgg accttgatct ccttgggtac
     1381 gccgatcagc atcgccgcct ccggattgtt gttctggggc gggattgtgc ccgctgttgg
     1441 acttcaccgc gacggcgcgt actgctcagt gcgattggag ttttcgacgg acgatctgcc
     1501 aggcgacgag cccgatgcac atcatgccga acgacgccac ggccagcccc agggttgaat
     1561 gcatcaccgc gggtgcgagc gcgccagcta ccagcgcgtt ggccacgctg ccgacgactg
     1621 cctgcagtga cgaggcgagc ccgcgtcgtt ccggcaccag gtcgagcacc atgatcgtga
     1681 ccaccggcac catcagcgac cagccgaaag cgaacagccc gatcagcggc agcgcccacc
     1741 atgcctgcgc ggtgaacagc gcattcgcga gcacgttcac gatcgacacg accaccatga
     1801 ccgtgaagcc gtagcggatc tgcttgcgcg gcctgatgcg ccccgccagc tgccccgaca
     1861 gccatgcgcc gcccatgatg ccgccgatgg tcatgcagaa gaaccagaag aactgggtcg
     1921 gtgccaggcc cagcagttcg ccgaggaagg cgggggcgga cagcacgtaa aggaacatgc
     1981 cgttgaacgg tatgccgctg gccagcgcca gcaacatgaa gttgcgatgc cccagcagct
     2041 gcgcatagcc gcggaagagg ttgctcacgt tgaacggctg gacgtcccgc ttgtgcagcg
     2101 tctcgggcag cacacgccag ttcgccagcc acagggccag ccccacgccc gcgagcagcc
     2161 agaagatcga gcgccagccg atgttgacgt agaggagtcc gccgatcagt ggcgcaaccg
     2221 cgggcgcgac gccgaagaag atcgtcacct gcgacatcac acgctgcgcc tgtgctggcg
     2281 ggaacatgtc gcggatgatc gcgcgcgaca cgacgatgcc tgcacccgtc gccaggccct
     2341 gcatcgcgcg gaagaacacc agctggccga tcgtctgcga gagcgcgcaa ccgactgaag
     2401 cgacggtaaa ggcggccaca ccccacagca cgaccggtcg ccggccgtag gcgtcggcca
     2461 gtgcgccgtg gaacaggttc atcgccgcaa atccgaacag ataggccgac agcgtctgct
     2521 gcatctgcag cgtcgtggcg tcgagcgatg tgcggatcgt cgcgaaggcc ggcaggtagg
     2581 tgtcgatcgc gaatggccct agcatgccga ggcatgcgag cagcagcgaa agcgcccacg
     2641 ccggagcctt ccagaggtgg gcggattcag gcttcaccgg cggtgcgtgc agaacgccgt
     2701 ggatagaaga ggcgccgatg ggggcgtctc ggacatcgat gtgccgctaa atcggttcaa
     2761 gcctttgtcg ctgtcgaatc gagcggcgcc ttgagtgcaa cctgtgcggg gtaacgctct
     2821 ccggcgaggt cgacggtaaa gatgcccgac tcgatccacg ccctttccgc cgggccttgt
     2881 gcgttggaga caatgcccag cgcgaccggc ttgcagatcg tgtgtccgaa cgctgccgac
     2941 gtgacggagc cgacgggtac gtcgttgcgc aagatgagtt cgccgctcca caggttggtg
     3001 tgaggcgcgt cgacgacaag cgacacgatg cgtctcgcga cagcggcggc accgccggcc
     3061 tccctgagtg cttgcagcgc gcgcctgccg cggaactcgg caggtttttc gagactgacc
     3121 gcaaaggcaa gtccggcctc gaaagggttg atgtccggcg tcagttcgcg gccccacgcg
     3181 cggtagccct tttcgagccg cagcgaatcg atcgcgtagt agccggcgtt cattggcccg
     3241 agcgcgaggc cgagatcacg cccggccgac atcagacgtt catgcacgcc gaccgcgaac
     3301 tcgaccggcg tgtagagctc ccagccgagt tcgccgacat aggtcagacg cgtggcgcgc
     3361 accgtcgcgt aaccgagatc gatctcctgc gaggtgccga agggaaacgc ggcattggaa
     3421 aaatcggcgg tggagacgcg ttgcagcagc tcgcgtgaac gcggccccat caccgccagc
     3481 atcgcgagca tcggcgtgat gtcggtcacc tggacgttcg ccggcgccgg cagtgcggcg
     3541 atcgcgcgcc ggatcacgtc gcgatcgcgt accgcctggg cgctgccggt gacgatcagg
     3601 tattcgtctt gaccgagaca ggtgatggtg aagtcgctct catagccgcc acgctcgttg
     3661 agcatgcccg tatatatcgt gcggccgggc tcgagcggaa catcgttggc gaccagccat
     3721 tgcagcacgg tgtgcgcgtc gcggccctgc acgagcagct tcgcgaacga actcatgtcg
     3781 aaaacggcca ccgcttcgcg gcaggcgcga tgttcggcgg ccacccacgg atgccagttc
     3841 tggtggcccc agccgtattc gatctgcgcc tgcgcgggcg atggcgcaaa gaaattggcg
     3901 cgctcccagc ccatctttga gccgaaacac gcgccggcgt cgcgcagttg cgcatacagc
     3961 ggcgagcgcc ggaacggacg ggccgattcc agttcacggt tcggccacgg catcttgtag
     4021 tgcatgccga gcacctcggc gatgcggtca ggcagccagg tctcgttggc gttgaagctc
     4081 gcgaagcggc gactgtcgac cggccagagg tccatggcgg gctcgccctg catgatccat
     4141 tcggccagtg ccttgccggc accgccggcc gaggcgatcc ccatcgaatt gaagccgcag
     4201 ccgacataca ggttgtcgat acccggcgcc gggccgacga tgaagttgtt gtccgcggta
     4261 aacgactcgg ggccgttgta gaactgctta acctgcgcgt tctcgagcga tggcacacgg
     4321 atcatcgcgt tgtcgagcag gatctggaaa gcatcccagt tgtcgggcag cagctggaac
     4381 tcgaagttct cgggaatccc gccgcgatgc cgtgcactct gcagccaggg cgtcgcttcc
     4441 ggttcaaagc cgcccatcag caggccgccg acctcttcct tgaagtagat gtagccgtcc
     4501 ggatcgcgca tgaccggcag gtcgcggtgc acgccgggga ccacgtcggt aacgatgtag
     4561 tagtgctcgc aggaaaatag cggcaccgcg accccgagct cccgtccgag cgcgcgcgac
     4621 cactggccgc cgcacagcac gacgatgtcg gcggcgatgc gcccggcttc gccgtccttg
     4681 ctgatccatt ccagatgcga aatgcgcttg ccggacccgg tctgcgttgc gtcgccggtg
     4741 acgcgcaccc cttcgcagat gcgtacgccg cggttgcgcg cgcccttggc gagcgcgagc
     4801 gtcaggtcag ccggattcgc cttgccatcg cccggcagcc acacggcacc ctggagatcg
     4861 cctgtttcca tgatcggata cagcgcgccc gcctcgctcg gggaaaggat gtcgcagctg
     4921 accccgtaag cctgcgcgac cgcggccgtg cgcttcagct gaatcatgcg gtcggccgtg
     4981 cgcgccaccg ccagcgatcc gcactgcttc cagccggtcg aaagccccgt ctcgccctcg
     5041 agtcgcgcat acagatcggt cgagtagcga atcagccgcg tcatgctctc atgcgtgcgc
     5101 aactgaccga ccagccccgc cgcgtgccac gtcgtccccg atgtcagccg cccctgttcg
     5161 agcagaacga catcggtctc gccgaaggaa ctcaggtggt acgccaccga gcaccccatg
     5221 atcccgccgc caatcaccac gatccgcgcc gcctgcggcg gccgaacctg cgtcgtcagt
     5281 gcctcgtccg ccactcgtgt ctccattggg ttcttgtcgt tatcctgcgg gcagagcgcc
     5341 cggccgctcc gaagcgagcc cgcctattca gtcttgagcg gccgcttcgc ggccgtgtcg
     5401 tgaaggatgc acgtgcgacg cgcagcgtcg cgtacatcct ttacgcctgc ttgaggtgcc
     5461 acgccttcag ccgcgtgaag gtctcgatgc cgtaggtcga cagcgtcagc ccgataccgg
     5521 agtgaccgac gccgctccac ggcaggcgcg gactgacgcg atcgcagcag ttccagtag
//
LOCUS       DATIRT010000112         6033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1685568, whole genome shotgun sequence.
ACCESSION   DATIRT010000112 DATIRT010000000
VERSION     DATIRT010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6033
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1685568"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..1869
                     /locus_tag="VLN25_02745"
     CDS             190..1869
                     /locus_tag="VLN25_02745"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HSQ09522.1"
                     /translation="MTQLFISYARENRETAERVAHALGEIGIDVWWDRDIPGGSEFAE
                     VIEQRLSEARVVIVLWSESSTRSGFVRDESMRALEAGKLLPVRIEVVVPPLGFGQIHA
                     LDLLDWDGAADDPAFVGVVNEVRQRLGQAPIARPHPPQPRPRWQRRAIIAAVAVLPAI
                     VAAAGGWHFYQLSESRRNLRLGLEEHFGREPNLQAARNYYLDALEYVSDNARARYYLG
                     HVYAQLGEIELARTSFERAVRDRDGLDDAQLADASTRVAALSPTVEPTALVRASSPST
                     APKDVIALSPPPPTAAKTVQPGAKSTGSTTLSTTVPSFAGSSGAASVTRLPRIAPSPD
                     LRRRALALVDQMFSDDSQTRITSTTSLVTDPDLLSDAVPLAVEQALDRLGAARGDPSA
                     SVQSGVVNTLVLLQSALPGTLVVNRGDIERLLKRAAPIGKYTAAQAEKVQTLLKGAAD
                     RRPLAFIQIANEAQRPMAEALVVRLRAAGYDAPGIEMVGERAPASSQVRVQGKSERGF
                     ARWIAKVVGDADGEAVAVQTLRNVNPKIDTFEVWFDRDLCTPGRSAPQCAR"
     gene            complement(1889..2707)
                     /locus_tag="VLN25_02750"
     CDS             complement(1889..2707)
                     /locus_tag="VLN25_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSQ09523.1"
                     /translation="MSISVVVTTYNRPDALRAVLDGLGAQTDRAFEVLVADDGSRDDT
                     RELVESFARGTSVPVRHVWQEDRGFRAGAARNRATAMAYGDYVVFLDGDCVPRPQFIE
                     RHRRLIRRSWMVAGNRVMLSESFTQEALAAHMPLHEWTDNQWRAAHRRGDINRTLPLR
                     HLALGPLRRLAASHWQRVRTCNLGVWRRDLKRVNGFDEVFEGWGYEDSDLAVRLINAG
                     VRRKEGAYATGVLHLWHHENDRRAEGDNWNRLQQRIRDHATWAERGLDQYPGIT"
     gene            complement(2728..3516)
                     /locus_tag="VLN25_02755"
     CDS             complement(2728..3516)
                     /locus_tag="VLN25_02755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSQ09524.1"
                     /translation="MARISVYIIAYNEADKIEAALKSVTWADEIVVADSFSTDGTVEI
                     ARKYTERVLQLPFEGFGKLRNDVLDRLEGDWIFSLDADERCTEAAAAEIRRVVAQPDA
                     SDAYRVPRRNFLLGRWIRHSGWYPDYRQPQLFRRGKLRYTADAVHESYVLDGTLGTLT
                     EPIAQVPFKDLAQIVHKMQRYSTLGVERLAQRRTRPSMGRALAHGLGAFLRHYIVRLG
                     FLDGWAGFVIAFSNFEGTFYRYAKYTERMQNLTREPPLPPSLKN"
     gene            3655..5433
                     /gene="msbA"
                     /locus_tag="VLN25_02760"
     CDS             3655..5433
                     /gene="msbA"
                     /locus_tag="VLN25_02760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HSQ09525.1"
                     /translation="MSSAHPRLLDDPNLRRLLRMVLSHRRLLVYGLIATAISAGTEPV
                     IARLAGVLTDQAMFQKDFASVIWMPIAFVGLFIVRGLAIFGGSYLLNRVSQAVLVDLR
                     MQMFDRMLHWPTSTFENTPSGIVISKFVNEASNALNLAAEVMSSAVRDSLIVIGLLVM
                     LFYYNWQLTLLTMIVAPPIGIALRAFSRRLRRLNLENQEMLGEMTRAVQEAHEGQRVV
                     KLYEGADYESGRFRQINEKLRGFAMRMQVAWSAATPIAQVLGAIGVAIVMTVALWQAK
                     TTRMSPGDFATFLAAALLLLPALRHLSSLNGPLARMAAASDSVFRMIDTAPEADSGTR
                     DIGRAHGEVEFRRVSFRYGGADADALTDFSLLVRPGEMVAFVGPSGAGKTTAVHLVPR
                     FVRPTAGEILLDGVPLEELTLDSLRRQVALVSQDVVLFDDTIAANIAYGAQRNATVER
                     IREAADAAYLLPFIDSLPAGFETRIGENAVKLSGGQRQRLSIARALLKDAPILLLDEA
                     TSALDSESERYIQASLQRLMNGRTTFVVAHRLSTIERADRIVVLERGRVVEIGSHREL
                     IGRGGLYARLHGIQFASTDTPLEEAQ"
     gene            5430..6017
                     /locus_tag="VLN25_02765"
     CDS             5430..6017
                     /locus_tag="VLN25_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241139.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HSQ09526.1"
                     /translation="MTPPTRPISRWPVPELAALPEDIRLRIKEVQDKAGFVPNVFLTL
                     AHRPEEFRAFFAYHDALMLRDSGLSKGDREMIVVATSGANDCVYCVVAHGAILRVYEK
                     NPLVADQVATNYRKADITPRQRAMLAFALKVATDSAAIEPLDFEPLRAHGFSDEDIWD
                     IGAIAAFFALSNRMANLISMRPNDEFYLLGRLPRK"
BASE COUNT         1001 a         1951 c         2013 g         1068 t
ORIGIN      
        1 cgacccgacg atcgactgat cgccgatctg cgcggccagg cccgtgtgcg ccgggcgagc
       61 ttcagttaca gcaactgagt cattgcggag ccgccccgct gcgcgctcgc gcgtcgaggg
      121 gatggcgcga tcgtcattgc aagcgccatt gtccgtgaca ccggaccgct ccaagcgctt
      181 gcaacgcact tgacccagct tttcatcagc tacgcccgcg aaaaccgcga aactgcggag
      241 cgagtcgcgc acgcgctcgg tgaaatcggc atcgatgtct ggtgggaccg cgatattccg
      301 ggcggatcgg aattcgccga ggtcatcgag cagcgcctga gcgaagcacg ggtggtgatc
      361 gtgctgtggt cggagtcgtc gacccggtcg ggttttgtgc gtgacgagtc gatgcgcgcg
      421 ctcgaagccg gcaagctgtt gccggtgcgt attgaagtcg ttgtgccgcc gctcggcttc
      481 ggccagatac acgcgctgga cctgctcgac tgggacggtg cggctgacga tcccgctttc
      541 gtcggggtgg tcaatgaagt gcgacagcgg ctcggccagg ctccgattgc tcgtccgcat
      601 ccgcctcaac cgcggccgcg ctggcagcgg cgggccatca tcgccgcggt cgctgtgctg
      661 ccggcaattg tggctgcggc cggtggctgg cacttctatc agctgagcga gtcccgccgc
      721 aatctgcgac ttggactcga ggaacacttc ggtcgcgagc ccaacctgca ggcggcgcgc
      781 aactattacc tcgacgcgct cgaatacgtc tcggacaatg cgcgggcgcg ctactacctc
      841 gggcacgtct atgcgcaact gggcgagatc gaactggcgc gcacgtcgtt cgagcgagcg
      901 gtgcgcgatc gcgacgggct cgacgacgcc cagcttgcgg acgcctcgac gcgcgtagct
      961 gcgctgtcgc ccacggtcga gccgaccgcg ctggttcgcg ccagctcgcc atcgacggca
     1021 ccgaaggatg tcattgcgct ttcgccgccc ccgccgactg cggcaaaaac agttcagcca
     1081 ggggctaagt cgacaggttc gacgaccctg agcacgacgg taccgagttt cgcggggtcg
     1141 agcggggcag catcggtcac acgactaccg cggatcgcgc cgagtccgga tcttcgccgc
     1201 cgggcgttgg ccctggtgga tcagatgttc agcgacgaca gccagacacg catcacatcg
     1261 accacctcgc tcgtgaccga ccccgacctg ctgtcggacg cagtgccact cgcggtcgag
     1321 caggcgctgg atcggttggg tgccgcgcgc ggagatccct cggcgtcggt ccagtcgggc
     1381 gtcgtgaata cgctggtgct gctgcaatcc gcgctgcccg gcacgctggt ggtgaaccgg
     1441 ggcgacatcg agcggctgct caagcgcgct gcaccgattg gcaagtacac cgctgcgcag
     1501 gccgaaaaag tgcagaccct gctaaagggc gcggccgacc ggcggcccct ggccttcatt
     1561 cagattgcca acgaggcgca gcggccgatg gccgaggcgc tggtggtgcg gctgcgcgcc
     1621 gctggatatg acgcgccggg catcgaaatg gtgggcgaac gcgcgccggc aagcagccag
     1681 gttcgcgtgc agggcaagag cgagcgcgga ttcgcgcgct ggatcgccaa ggtggtcggc
     1741 gatgcggatg gcgaagcggt cgcggtgcaa acgctgcgca acgtcaatcc gaagatcgac
     1801 accttcgagg tctggttcga tcgcgacctg tgcacaccgg gacgcagtgc gccacagtgc
     1861 gcacgttagg aacaggccct gtgccgtgtc aggtgatgcc cgggtattga tcgagtccgc
     1921 gttcggccca tgtggcgtga tcgcggattc gctgctgcaa gcgattccag ttgtcgcctt
     1981 cggcgcgccg gtcgttctcg tgatgccaca gatgcagcac gccggtcgcg tacgcccctt
     2041 ccttgcggcg caccccggca ttgatcagcc tcacagcgag gtccgaatcc tcgtagcccc
     2101 agccttcgaa gacctcgtcg aagccgttga cgcgcttgag gtcgcgacgc catacaccga
     2161 gattgcaggt gcgcacgcgc tgccaatgcg atgccgcgag tctccgcagc ggtccgagcg
     2221 cgaggtggcg cagtggcaac gtccggttga tgtcgccgcg tcgatgcgca gcgcgccact
     2281 ggttatcggt ccactcgtgc aagggcatgt gtgccgccag cgcctcctgg gtgaacgact
     2341 ccgacagcat cacacgattg ccggcgacca tccagctccg cctgatcagt cttcggtgac
     2401 gctcgatgaa ttgaggtcgc ggcacgcagt caccgtcgag gaagacgaca tagtcaccgt
     2461 aggccatggc cgtcgcacgg ttgcgcgccg cacctgcgcg gaacccgcgg tcctcctgcc
     2521 agacgtgccg caccggcacc gaggtcccgc gggcaaagga ctcgaccagt tcgcgcgtgt
     2581 cgtcgcgcga gccgtcatca gcgaccagta cttcgaacgc ccggtccgtc tgcgcgccca
     2641 ggccgtcgag gacggcccgc aatgcatcgg gtcggttgta ggtggtcacg accaccgaaa
     2701 tggacatcgc ggattccggg cggggtatca gttcttcagg ctgggtggaa gcggcggctc
     2761 gcgtgtcagg ttctgcatgc gttcggtgta cttggcatag cgataaaacg tgccctcgaa
     2821 gttcgagaaa gcgatgacga acccggccca gccgtcgagg aagccaagcc gcacgatgta
     2881 gtggcgcaag aatgctccga gtccgtgagc gagcgccctg cccatcgatg gtcgcgtgcg
     2941 gcgctgggcg agtcgttcga cgccgagcgt cgagtaccgc tgcatcttgt gcacgatctg
     3001 tgcgagatcc ttgaatggca cctgggcaat cggttcggtc agggttccca gtgttccgtc
     3061 gaggacgtag gactcgtgca ccgcatcggc cgtgtagcgc agcttgccgc gccggaacaa
     3121 ctgcggctgc cgatagtccg gataccagcc ggaatgacgg atccagcgtc cgagcaggaa
     3181 gtttcgtctc gggacgcggt aggcatcaga cgcatcaggc tgcgcgacga cgcgccggat
     3241 ctccgctgcg gccgcctcgg tgcagcgttc gtcggcatcg agcgagaaga tccagtcgcc
     3301 ttcaagccgg tcgagcacgt cattgcgcag cttgccgaag ccttcgaacg gcagttgcag
     3361 aacgcgttca gtgtacttgc gggcgatctc gactgtcccg tcggtcgaaa acgagtccgc
     3421 cacgacgatt tcgtcggccc aggtgacgct tttcagcgcc gcctcgatct tgtcggcctc
     3481 gttgtacgca atgatgtaga cggaaatgcg cgccattgcc gctccagcgt cctgcttcag
     3541 cattctgccg caagcttaac ggcagcgttg tcaacgagta tgcaagcgcg ttcgcataca
     3601 ctacgcattg cgtgcgcacg ggctcggtct gcgggcgggc gccgcctccg cgacatgagt
     3661 tcagcccatc cccgcctgct cgatgatccg aacctgcgcc gcctgctgcg catggtgctg
     3721 tcgcatcgcc ggctgctggt gtacgggctc atcgccacgg ctatcagtgc cggcactgaa
     3781 ccggtgatcg cgcgcctggc cggcgtcctg accgaccagg cgatgttcca gaaggacttt
     3841 gcgtcggtca tctggatgcc gatcgcattc gttggacttt tcattgtccg cgggctggcg
     3901 atcttcggag gttcgtacct gctgaaccgg gtctcgcagg cagtgctggt cgacctgcgc
     3961 atgcagatgt tcgatcgcat gctgcactgg ccgacgtcga ccttcgagaa cacgccgtcg
     4021 ggcatcgtga tctcgaagtt cgtcaatgaa gcgagcaatg cgctcaatct cgccgccgag
     4081 gtgatgagct cggccgtgcg cgattcgctg atcgtgatcg gcctgctggt catgcttttc
     4141 tactacaact ggcagttgac gctgttgacg atgatcgtcg cgccgccgat cggtatcgcg
     4201 ctgcgcgcgt tcagccgccg cctgcgccgc ctcaacctcg aaaaccagga gatgctgggc
     4261 gagatgacgc gcgcggtcca ggaggcgcac gaaggccagc gcgtggtcaa gttgtacgag
     4321 ggtgcggact acgaaagcgg acgcttccgc cagatcaacg agaagttgcg cgggttcgcg
     4381 atgcgcatgc aggtcgcctg gtcggcggcg acgccgattg cgcaagtgct cggtgccatc
     4441 ggcgtggcga tcgtgatgac ggtagcgctg tggcaggcaa agaccacgcg gatgtcgcca
     4501 ggcgatttcg ccacgtttct cgcggcggcg ctgctgctgt tgccggcgct gcggcacctg
     4561 tcgtcgctga acggcccgct ggcgcggatg gccgcggcat ccgacagtgt cttccggatg
     4621 atcgacacgg ccccggaagc cgacagcggg actcgcgata tcggtcgggc gcacggtgaa
     4681 gtggagttcc gtcgcgtttc gttccggtac ggaggtgccg acgccgatgc actgaccgac
     4741 ttcagtctgc tggtgcggcc gggcgagatg gtcgccttcg tcggaccctc cggcgctggc
     4801 aagaccacgg cggtccatct tgtgcctcgc tttgtacggc cgacggcggg cgagatcctc
     4861 ctcgacggag ttccgctgga ggaactgacg ctcgacagcc tgcgccgcca ggtcgcgctg
     4921 gtgtcgcagg atgttgtgct cttcgacgac accattgctg ccaacattgc ctacggcgcg
     4981 cagcgcaacg ctacggtcga gcgcatacgc gaggccgccg acgcggctta cctgctgcca
     5041 ttcatcgatt cgctgccggc aggtttcgag acacgcattg gcgagaacgc agtcaagctc
     5101 tcgggcgggc agcgccagcg cctgtcgatc gcgcgcgcac tgctgaagga cgcgccgatc
     5161 ctgctgctcg acgaagcgac gtccgcgctc gactcggaat ccgagcgcta catccaggcg
     5221 tcgttgcagc ggctgatgaa cgggcggacc acgttcgtcg tcgcgcaccg gctgtcgacg
     5281 atcgagcgcg ccgatcgcat cgtcgtgctc gagcgcggac gggtggtcga aatcggctcg
     5341 caccgcgaat tgattggccg gggcggtctg tacgcgcgac tgcacggcat ccagttcgcc
     5401 tcgaccgaca ccccgctgga ggaagcgcaa tgaccccacc cacccgaccg atcagtcgct
     5461 ggcccgtgcc cgaactggca gcattgccgg aggacatccg cctgcgcatc aaggaagttc
     5521 aggacaaggc cggcttcgtg ccgaatgtgt tcctgacgct cgcgcatcgt cccgaggagt
     5581 ttcgcgcctt cttcgcctac cacgacgcac tgatgctgcg cgactcgggc ctgagcaagg
     5641 gcgacaggga aatgatcgtc gtggcgacca gcggcgccaa cgactgcgtg tactgcgtcg
     5701 tcgcccatgg cgcgatcctg cgtgtctacg agaaaaaccc gctcgtcgcg gaccaggtcg
     5761 cgaccaacta ccgcaaggcc gacatcacac cgcgccagcg cgcgatgctc gcgtttgcgc
     5821 tcaaggtcgc gaccgactcg gcggcgatag agccgttgga tttcgagccg ctgcgagcgc
     5881 acggtttcag cgacgaggac atctgggaca tcggcgccat cgcggcgttc ttcgcgctgt
     5941 cgaaccggat ggcgaacctg atcagcatgc ggccgaacga cgagttctac ctgctcgggc
     6001 gcctgccgcg caagtgaccg ccggtgccga tcg
//
LOCUS       DATIRT010000113         2109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_210896, whole genome shotgun sequence.
ACCESSION   DATIRT010000113 DATIRT010000000
VERSION     DATIRT010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210896"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..170)
                     /locus_tag="VLN25_02770"
     CDS             complement(<1..170)
                     /locus_tag="VLN25_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HSQ09527.1"
                     /translation="MKTRAAVAWTAGKPLTIEEVDLEGPRAGEVLVEIKATGICHTDY
                     YTLSGADPEGLFP"
     gene            358..957
                     /locus_tag="VLN25_02775"
     CDS             358..957
                     /locus_tag="VLN25_02775"
                     /inference="COORDINATES: protein motif:HMM:NF017437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSQ09528.1"
                     /translation="MYERHFGLKQRPFSIAPDPRYLFMSERHREALAHLLYGVQGGGG
                     FVLLTGDVGAGKTTVCRCFLEQLPERCNVAYIFNPKLTVEELLKTVCAEFRIPYQHEG
                     PGVPTVKDYVDALNEFLLRTHAVRQTSVLIIDEAQNLSVRVLEQLRLLTNLETNERKL
                     LQIILIGQPELRAILARPDMEQLAQRVVARYHLEALTEE"
     gene            1060..2046
                     /locus_tag="VLN25_02780"
     CDS             1060..2046
                     /locus_tag="VLN25_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09529.1"
                     /translation="MPRRINLLCDRALLGAYADSKPTVDRRIIDKAAEEALDAVDLGK
                     IRRARFERASLVGIGLVVGAVLVGVVGLSLNRGERGAPRTELAANASPAPTVVAAAPA
                     AAPAPEPTAFQPVTMAPEPPKPAPFDLRKGFASLVGADKEAWQELGRLWNIAPGDGDP
                     CAAAEKQQVRCYRTGNATLALIRQLDRPGVLTLRDAANRPAYVMISGLSNDTATLTIG
                     GVDQTVPLVVLADYWRGEFATFWRPPPNYAGTVVDSLAGPAAHWLAQQLATALGDTGS
                     VGPRLDDAKLQSWIHRFQLAQGLPSDGSAGPITLMQLTRANGVDEPRLQKAT"
BASE COUNT          349 a          727 c          698 g          335 t
ORIGIN      
        1 gggaacagtc cctcgggatc ggcgcccgac agcgtgtagt agtcggtgtg acagatgccg
       61 gtcgccttga tctcgaccag cacctcgccg gcacgcggcc cttcgagatc gacttcttcg
      121 atagtcaaag gctttccggc cgtccaggcc accgccgccc gtgtcttcat ggcattcgcc
      181 ccgaattggt tgaccggaga ttgtgcccgc aagatcgttg cagtaggccg gcgttccggt
      241 ctgctgccgc agggagtcga ccggttcacc cacgcccacg ccgacccgcg cagcagtccc
      301 ggagacgcgt cttcacccgc acacaagtcc cgctcagggc cagtatcctt gccggcgatg
      361 tacgagcgcc acttcgggct gaaacaacgg cccttctcga tcgcgccgga tccgcggtac
      421 ctcttcatga gcgagcggca ccgcgaggcc ctcgcgcacc tgctttatgg cgtgcagggc
      481 ggcggcggct tcgtgctgct gaccggggac gtcggcgccg gcaagaccac cgtgtgccgc
      541 tgcttcctcg aacaactgcc cgagcgctgc aacgtcgctt acatcttcaa cccgaagctg
      601 acggtcgagg aattgctgaa gaccgtgtgc gcggagttcc gcatcccgta ccagcacgag
      661 gggccgggtg tgccgaccgt caaggactat gtcgacgcgc tgaatgagtt cctgctgcgc
      721 acgcacgccg tgcggcagac gagcgtgctg atcatcgacg aagcgcagaa cctttcggtt
      781 cgcgtgctgg agcagttgcg cctgctcacc aacctcgaga ccaatgagcg gaagctcttg
      841 cagatcatcc tgatcgggca gccggagttg cgcgcgatac tggcacggcc cgacatggag
      901 cagctcgcgc agcgcgtggt cgcccgctac cacctcgagg cgctcacgga ggagtagacc
      961 gggcactatg tcagccatcg cctgggcgtc gcgggattgc gccaggccaa tcccttcgat
     1021 caacgcgcga ttcggcgcgt cttccgttat tcacgcggag tgccacgccg cattaacctg
     1081 ctgtgcgatc gcgcgctgct gggcgcctac gcggacagca agcctaccgt cgaccgccgc
     1141 atcatcgaca aggcggccga ggaagcgctg gatgccgtcg atctcggcaa gatccgacgc
     1201 gcgcgcttcg agcgcgcgtc ccttgtgggc atcgggctgg tggtcggcgc ggtgctggtc
     1261 ggagtggtcg gcctctcgct gaaccgcggc gaacgcggcg cgccgcgcac tgagctggca
     1321 gcgaacgcga gccccgctcc gacagtggta gccgccgcgc ccgcggctgc gccggcacca
     1381 gagccgacgg cttttcagcc ggtgaccatg gcgccggagc ctccgaagcc cgcgcccttc
     1441 gacctgcgca agggctttgc gtcgctcgtc ggtgccgaca aggaagcgtg gcaggagctg
     1501 ggtcgcctat ggaacattgc tccgggcgat ggcgatccct gcgcggcggc ggaaaagcag
     1561 caggtccgct gctaccgcac cggcaacgcg acgctggcgc tgatccggca gctggaccgt
     1621 ccgggcgtgc tgacgctacg cgacgcagcc aaccgccctg cctacgtgat gatcagcgga
     1681 ctgtcgaacg acacagcgac gttgacgatc ggcggcgtag accagacggt cccactcgtc
     1741 gtgctggccg actactggcg cggagagttc gcgacgttct ggcggccgcc accgaactac
     1801 gcgggcacgg tcgtggacag cctcgcgggg cccgccgcac actggctcgc gcagcagctc
     1861 gcgaccgcgc tgggcgacac cggctcggtc ggtccacggc tcgacgacgc caagttgcaa
     1921 agctggatcc atcgcttcca gctcgcccaa gggctgccgt cagacggcag tgccggaccg
     1981 atcacgctga tgcagctcac tcgcgcgaac ggcgtggacg agccgcgcct gcagaaagcg
     2041 acttagtcca atgtcactga tcctcgacgc actgcgcaag gcggacgcgg aacgcgagcg
     2101 cggctcggt
//
LOCUS       DATIRT010000114         2515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_112205, whole genome shotgun sequence.
ACCESSION   DATIRT010000114 DATIRT010000000
VERSION     DATIRT010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112205"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VLN25_02785"
     CDS             <1..407
                     /locus_tag="VLN25_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSQ09530.1"
                     /translation="YWTAIHMVPYFRTRGGGCFVNIASTAGVRPRPGLTWYNGSKGAV
                     ITTSKSMAAELGPDNIRVNCVNPVIGATGLVEEFMGMPDTPENRKKFLATIPLGRYST
                     PDDVAAACVFLASDVARFITGASLEVDGGRCV"
     gene            complement(453..2135)
                     /locus_tag="VLN25_02790"
     CDS             complement(453..2135)
                     /locus_tag="VLN25_02790"
                     /inference="COORDINATES: protein motif:HMM:NF003715.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HSQ09531.1"
                     /translation="MDVLNQTLLIAGLLCFVAIALSAASQKIGVPSLLVFLAVGLFAT
                     ELPQVRWVHIELETAVLVGNLALAVILLDGGLRTRLATFRMVAAPSLTLATFGVILTA
                     ALVGVAGAWLLDLDWRYGLLLGSIVGSTDAAATFALLGNSGLRLNERVESTLEVESGI
                     NDPMAVFLTLGTIELIRSPARSIADLLPMFLQQLGIGVLVGVALGYLLSLAVARVRLD
                     EGLYALLIQSGGLATFALTNLLGGSGFLAIYLAGMLIAHRRAHVGEDVLRVSDGFAWL
                     AQAGMFLVLGLITDVRGLLDVAGPALLVAAVLLFIARPLAVAACLLPFRYPAREIAFI
                     GWIGMRGAVPIVLGLFPLVAGIPQAQLLFHIAFFIVLLSLLLQGGSLPVAARLARVRA
                     GRPRAALATAPLDGGVQVREVVQFVVAEGAPATRRALDELDWPEGARLVELERAGRIV
                     EPTELRSGDLVAVVAPAEQVGALEEIFAPAAVDGELTLAASATLGDLQDYYGVEIPEG
                     LQRDTTLAAYVSRLLRGRAASGDAVARGSLRLRVRESSAGVVRSFSLLLKRR"
     gene            complement(2241..>2515)
                     /locus_tag="VLN25_02795"
     CDS             complement(2241..>2515)
                     /locus_tag="VLN25_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09532.1"
                     /translation="RENSIRAVLLRTAGVLCLREQLWGKAGSYLNESLQVSKQPPTLV
                     ALARLAEAVGDDAEAARHYREAALGFVLPAPAGPDSARPVLREHTL"
BASE COUNT          458 a          885 c          839 g          333 t
ORIGIN      
        1 tctactggac cgcgatccac atggtgccgt acttcaggac gcgcggcggc ggctgcttcg
       61 tcaacatcgc ctcgaccgcg ggagtgcgcc cccgtcccgg gctcacctgg tacaacggca
      121 gtaaaggagc cgtcatcacc acgagtaagt cgatggccgc cgagctcggg ccggacaaca
      181 tccgtgtcaa ctgcgtgaac ccggtgatcg gcgcgaccgg tctggtcgag gagttcatgg
      241 gcatgcccga cacacccgag aatcgcaaga aattcctggc gacgataccg ctcggccggt
      301 actccactcc ggacgatgtc gccgctgcct gcgtgttcct cgcttcggac gtagcccgct
      361 tcatcacggg cgccagcctc gaggtcgacg gtggccgctg cgtttagcgt tgccgaccag
      421 cgctgcgttt ggctttgccc ttgcctttgt acttagcgcc gtttcagcag caggctgaac
      481 gagcgcacca caccggcgct cgattcgcgc acccgcagcc gcaggctgcc ccgcgcgacg
      541 gcatcaccgc ttgccgcgcg tccgcgcagc aggcgtgaga cgtaggcagc gagcgttgtg
      601 tcccgctgca gaccctccgg aatctcgacg ccgtagtagt cctgcaggtc acccagggtg
      661 gccgaagccg ccagcgtcaa ttctccgtcc accgcggcag gcgcgaagat ttcttccagt
      721 gcaccgacct gctcggccgg cgccacgacc gccaccagat cgccactgcg cagctcggtg
      781 ggctcgacga tgcggccggc gcgctcgagc tcgaccaggc gcgcgccctc cggccagtcg
      841 agctcgtcga gcgcccggcg ggtcgcgggc gcgccttcgg cgacgacgaa ctgcaccacc
      901 tcgcgcacct gcacgccgcc gtcgagcggc gccgtggcaa gcgcagcgcg cggacgcccc
      961 gcgcgcacac gcgccagccg ggcggcgacc ggcagtgacc cgccctgcag cagcagcgac
     1021 agcaggacga tgaaaaatgc gatgtggaaa agcagctgcg cctgcggtat cccggccacc
     1081 aggggaaaga ggccgagcac gatcgggact gcgccgcgca tgccgatcca gccgatgaag
     1141 gcaatctccc gcgccgggta ccggaacggc aacaggcagg ccgccaccgc aagcggtcgc
     1201 gcgataaaga gcagcacggc agcaaccagc agggccggac ccgcaacgtc gagcaggccg
     1261 cgcacatcgg tgatgaggcc cagcacgaga aacatgccgg cctgggccag ccaggcaaac
     1321 ccgtcgctga cgcgcagtac gtcctcgccg acgtgggcgc ggcggtgcgc gatcagcatg
     1381 ccggccaggt agattgccag gaagccgctg ccgcccagca ggttggtgag agcgaaggtg
     1441 gccagtccgc cggactggat cagcagcgca tacaggccct cgtcaagacg cacacgcgcg
     1501 accgcgagtg agagcagata tcccagggcg acgcccacga gcacgccaat gccgagctgc
     1561 tgcaggaaca tcggcagcag atccgcgatc gatcgcgcgg gtgaccgtat gagttcgatg
     1621 gtgcccaggg tcaggaatac cgccatcgga tcgttgatgc cggattcgac ctcgagcgtc
     1681 gattcgacgc gttcgttgag gcgcaggccg ctgttgccca gcagcgcaaa cgttgccgcc
     1741 gcgtcggtcg agccgacgat cgaacccagc agcagtccgt agcgccagtc gaggtcgagc
     1801 agccaggcgc ccgccacgcc cacgagcgcc gcggtcagaa tcacaccgaa cgtcgccagc
     1861 gtcagcgacg gggccgcaac catgcggaat gtcgccagcc gcgtccgcag gccgccatcg
     1921 agcaggatca ccgccagcgc gagattgccg accagcacgg ccgtctccag ttcgatgtgc
     1981 acccaccgca cctggggcag ctcggttgcg aagaggccca ccgcgaggaa gacgagcagc
     2041 gacggcacgc cgatcttctg cgaagcggcg gacagcgcaa tcgccacgaa acaaagcaga
     2101 ccggcgatga ggagagtctg attgagcacg tccatgccgc gatttgatca cgacccgggc
     2161 atcggccctg cactggggtg gcgcggtgcc caatcagcgc gctggactcg cgccgatagc
     2221 aacgaaaacc cggcgaaggt tcagagcgtg tgttcgcgca gcacgggtcg cgcgctgtcg
     2281 ggtccggccg gtgcgggcag cacgaaaccc agggccgcct cgcgatagtg ccgcgccgcc
     2341 tcggcgtcgt caccgacggc ctcggccaga cgcgcgagcg ccaccagtgt cgggggctgc
     2401 ttgctgacct gcaggctttc gttgaggtag ctgccggcct tgccccacag ctgctcacgc
     2461 aggcacagca cgccggcggt gcgcagcagt accgcgcgga tagagttctc gcgcg
//
LOCUS       DATIRT010000115         6158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1095921, whole genome shotgun sequence.
ACCESSION   DATIRT010000115 DATIRT010000000
VERSION     DATIRT010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6158
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095921"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VLN25_02800"
     CDS             complement(<1..338)
                     /locus_tag="VLN25_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09533.1"
                     /translation="MNPGAGHPAVGAAAARSTGDLAAAALNGMREALKARVAVVQLEG
                     RIAARNLLRAALYVAAARLAVLAGWLALQVARVLALVASGLLSMLGASFVVALINSVI
                     AYVLFYVSQR"
     gene            complement(335..652)
                     /locus_tag="VLN25_02805"
     CDS             complement(335..652)
                     /locus_tag="VLN25_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09534.1"
                     /translation="MLETTYPKTTTADPVGSGTNFVDRLAQGAHHAVDQAAASAKPAL
                     EKLQESGAAAREVLAEKAEAAASMSDELMDSVRHYVRERPLTVLAGAVALGIVLAGIS
                     RSR"
     gene            827..1027
                     /locus_tag="VLN25_02810"
     CDS             827..1027
                     /locus_tag="VLN25_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HSQ09535.1"
                     /translation="MNWDRIEGNWKQFTGKVKEQWGKLTDDDIDRMAGQRDQLVGKIQ
                     ENYGVARDEAEQQVDDWSRRLM"
     gene            1110..1394
                     /locus_tag="VLN25_02815"
     CDS             1110..1394
                     /locus_tag="VLN25_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09536.1"
                     /translation="MNEAERKDLPQKSDAGNPDIQGEGNYDAARRHRQSVEDFLESND
                     PEELARSAAPADPREEKELEDAEKRGREGPGEDTEQDIAKRDRPMQSPKR"
     gene            complement(1451..1732)
                     /locus_tag="VLN25_02820"
     CDS             complement(1451..1732)
                     /locus_tag="VLN25_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09537.1"
                     /translation="MTWRTRPEETSNVLGAWALEVLNDLRLEWRCRPLIRHLSALALC
                     LVVVLVGVLERSTKRTSFATTVTRPGVAVAEQRAPARDLSRAEPPARRQ"
     gene            complement(1729..1935)
                     /locus_tag="VLN25_02825"
     CDS             complement(1729..1935)
                     /locus_tag="VLN25_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09538.1"
                     /translation="MKVKKRLGSSTTSEPALPNERDQRAARPAAPRSQGRRAYADATS
                     GRVDTDTRGSQLLQLAHRLKARRP"
     gene            complement(2029..5172)
                     /locus_tag="VLN25_02830"
     CDS             complement(2029..5172)
                     /locus_tag="VLN25_02830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HSQ09539.1"
                     /translation="MASSAAVGSNYRGTMLIPKVKWWKAQMAAVLLTLLAFAFTVYVL
                     GRIPEGRHPLLVLLLASALSAAYGGLIAGVVSTLLGVTLVAYVYEPAGSLLVVDPQDQ
                     TRIVLLALLGIVISVVFAALQKARREETKVVKRLHEEVAARDRIERSLTESRDSFELL
                     AASVPEIVFVATTDGQFEYLNSRWTEFTGLPRELSLGSGWRSAVHPDDLQAFDTQLVQ
                     AIQLMQPLELRLRLRSLEGAFRTTLIRASPSRRADGSLRWFGSATDIEDVARSSAALR
                     ASEERLKAVVAAAELGTFVWDAETDELLWSERARNILGIPMPGPRRLAEFVAQLAPTE
                     ADRVHRAMHDAIAQGEGQRFRTEIAIGQGPKARSVLVAGLVTERHGLGSNSHGLQCVG
                     AVLDQSRERSTAAQVVRLETSLQLAIESTRLGIWDVDLATDSITLSARAREIYGFSAD
                     AVVTRQAARSRILEEDLEGVEKCWRAALDPAGQGRLDVDHRVRRADGSVAWVSALGRA
                     HFVGSGSDRVAARIAGTVLDISDRKQAEQRLLAQEQQFLLTAEAITGLIYSTDLETGR
                     VERMRGLYDILGWERDEVPATVSWWHDQVHPDDMKQLEGDLKAAIAARQRRLSSRYRA
                     RHRSGHWRHLVDRAVVMYHDDGVPCRLVGCVQDVSEQVETTRSLVEADERKDRFLAVL
                     AHELRNPIAPLRNAVSILRTADFERPELWQSVEILDRQLLHATRLVDDLRDVGRIGRG
                     SLELRKAAVRFAEVVHTGVEAVRPAIEERGQQLKLALTLDDEPVLVDQVRIAQVIANL
                     VGNASKFSHTGDTIEVTATTLGSQVQITVEDHGKGIPPTALSTIFEMFAKLDRGNTEA
                     TEGLGIGLALSRHLVELHGGQVRAYSEGRGTGARFVVTLPVQRAPVDTTPSARGDKTR
                     ALKIVVADDNVDSVESLAALLTLAGHQVAVAHDGLTAVQLTADMQPDLAILDLGMPGI
                     DGYEAAARMRESPVGGDLVLVALSGYAQPADRQRSQQAGFDLHLAKPLDPDRLRDLLA
                     AVGTGRVQFRDNYAMGT"
     gene            complement(5301..5468)
                     /locus_tag="VLN25_02835"
     CDS             complement(5301..5468)
                     /locus_tag="VLN25_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020565957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HSQ09540.1"
                     /translation="MLYWAAVFFVIALVAALFGFGGIAAGASSIAQWIFFLFIIIAVV
                     SFIVGLVRRPH"
     gene            5656..>6158
                     /locus_tag="VLN25_02840"
     CDS             5656..>6158
                     /locus_tag="VLN25_02840"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HSQ09541.1"
                     /translation="METTTPKPAAAPDAAVTPSRSRARHIERTALVVIALIVTCAALV
                     FARTLLIPIVVAVMLSLLLRPLVLLLARYLRIPDAIGSLAIVALGLTGVVWFGTYLYE
                     PAEKLVKMGPGEVRQLERKLRDLARPVQAIRGATDKVTKLAEAADPTDKRREVVMQSG
                     GFLQWFET"
BASE COUNT         1152 a         2002 c         2010 g          994 t
ORIGIN      
        1 tgccgctgtg acacatagaa aagaacgtag gcaatgacgc tgttgatgag tgcgacgacg
       61 aaactcgccc ccagcatcga gagaagtccc gacgcaacga gcgccagcac gcgggcgacc
      121 tggagcgcca gccagcctgc cagcactgcg agccgcgcag ccgcgacata aagggccgca
      181 cgcaggaggt tgcgcgcggc aatccgtcct tccagttgca ccacggcaac gcgcgccttt
      241 aatgcctcgc gcataccgtt caatgcagcg gcggccagat caccggttga acgggccgcc
      301 gccgcaccga cggcgggatg tcccgccccc ggattcatcg cgaacggctg atgccggcaa
      361 ggacgatgcc gagtgcgact gcaccggcga ggaccgtcag agggcgctct cgcacgtaat
      421 ggcggacaga gtccatgagc tcgtccgaca tggacgcagc cgcctcggcc ttttcggcaa
      481 ggacttcgcg cgccgcagca ccggattcct gcagtttttc gagggcgggc ttggcgctgg
      541 ctgcagcctg gtcgaccgcg tggtgtgcac cctgcgcgag gcgatcaacg aagttcgttc
      601 cgctgccgac cggatcggca gtcgtggtct tcggataggt ggtttcaagc atggcaattc
      661 cctttcggtg agtgattgaa ctgggtgcaa cgtcgcaatg agcacccgcc caatagccca
      721 aggcaatgca cgtgcctgac catccaatgg gtacagcgct tgccgcaatc tcccgcggac
      781 gctcaatgcg tcggctcttt cctcccttaa tgcaaaggag tcagcaatga attgggaccg
      841 tatcgaaggc aactggaagc agttcaccgg caaggtcaag gagcagtggg gcaagctcac
      901 tgacgacgac atcgatcgca tggcgggtca acgcgatcag ctcgtcggca agatccagga
      961 gaactacgga gttgcccgcg atgaggcgga gcagcaggtt gacgactggt cgcgtcgcct
     1021 gatgtaagaa agccgcaaac acaacgcgcg gctggcgggc agttcgctcc gtcggtcgcg
     1081 caccttcgac cacgaacagg agtgcagcca tgaacgaagc agaacggaaa gacttgccgc
     1141 agaagtcgga tgccgggaac ccggacatcc agggcgaggg aaattacgat gcagcccggc
     1201 gccaccggca gagcgtcgag gactttcttg aatcgaatga tcccgaggaa ctggcgcgtt
     1261 cggccgcacc cgcggatcca cgcgaggaga aggaactcga agacgcggaa aagcgcgggc
     1321 gagaaggtcc gggggaggac accgagcagg acatcgctaa gcgtgatcgc cccatgcaat
     1381 cgcccaagcg ctgacaggca cggctcgtcc ccgggcacca cgcccggaga cgacgcccac
     1441 aatcatgtag ttactgccgg cgggccggcg gctcggcgcg actcagatct cgcgcgggag
     1501 ctcgttgttc tgcaaccgca acgccggggc gggtcaccgt cgtcgcgaac gacgtgcgtt
     1561 ttgtcgatct ctcgagcacg ccgacgagca ctaccaccag acacagcgcc agcgcgctga
     1621 gatgccggat caatggtcgg cagcgccact ccagccggag gtcgttcagc acctcgagcg
     1681 cccaggcccc tagaacgttc gaagtttctt caggccgtgt acgccaggtc atggccggcg
     1741 cgccttcaga cggtgcgcca gttgcagcaa ctgggatcca cgcgtgtcgg tgtcgacacg
     1801 cccggaagta gcgtccgcat atgcccggcg gccctgtgaa cgcggtgcgg ctggacgcgc
     1861 ggcgcgctgg tcacgctcgt tcggcagcgc cggttcggac gtcgtcgatg agcccaatcg
     1921 cttcttcact ttcatgggaa cccctggatg agtgatttct cacggcaagg gacatacccg
     1981 ttcgagaacc ccgtcgggga cggaagcgcg gctatcagac gacgggactc aggttcccat
     2041 ggcgtagttg tcgcggaact ggacgcgccc ggtgccgacg gctgcgagca ggtcgcgaag
     2101 tcgatcggga tcgaggggct tggcgaggtg caggtcgaag ccggcttgct gtgagcgctg
     2161 tcgatcggcg ggttgcgcat agcccgagag ggcgaccagg accagatccc cgccgaccgg
     2221 cgactcgcgc atgcgcgcag ccgcctcgta gccgtcgatg ccaggcatgc cgaggtccag
     2281 gattgccagg tccggctgca tgtcggccgt cagttggacc gcggtcaacc catcgtgcgc
     2341 gaccgcgacc tggtggccgg caagcgtcag cagcgccgcg agcgattcga ccgagtcgac
     2401 gttgtcatcg gcgacgacta tcttcagcgc gcgcgttttg tcaccgcgag cggacggcgt
     2461 cgtgtcgacc ggagcccgct gcacggggag cgtgacgacg aagcgcgcac ccgtaccgcg
     2521 accttcgctg taagcgcgaa cctggccgcc atggagctcc accagatgac gcgacagcgc
     2581 gagcccgatg ccgaggcctt ccgttgcttc ggtattgccg cggtcgagct tggcgaacat
     2641 ctcgaagatc gtcgacagtg ctgtcggcgg tatgcctttg ccgtgatcct ccaccgtgat
     2701 ctgcacctgg ctgcccagcg tggtggccgt cacctcgatg gtgtctccgg tgtgcgaaaa
     2761 cttcgaagca ttcccgacca ggttggcgat cacctgcgca atgcgtacct gatcgaccag
     2821 gaccggttcg tcgtcaaggg tcagggcgag cttgagttgc tggccacgct cctcgatcgc
     2881 agggcgaact gcttctacgc cggtgtgcac aacttcggca aaccgcacgg ccgccttgcg
     2941 cagttcgagg ctcccgcgcc cgatgcggcc gacgtcgcgc aggtcgtcga ccagccgcgt
     3001 cgcgtgcagt agctgccggt cgaggatctc gacgctctgc cacagttccg ggcgctcaaa
     3061 gtcggccgtg cgcaggatcg aaacggcgtt gcgcagcggc gcgatcgggt tgcgcaactc
     3121 gtgcgcgagc acggcgagga aacgatcctt gcgttcgtcg gcttcgacca gtgaacgggt
     3181 ggtttcaacc tgctccgaga cgtcctgcac gcaacccaca agccgacagg gcacaccgtc
     3241 gtcgtggtac atcaccaccg cgcggtcgac cagatgtcgc cagtgcccgc tgcggtgccg
     3301 ggcccggtaa cgcgagctaa gtcgtcgctg gcgcgcggca atggcagcct tgaggtcccc
     3361 ctctagttgt ttcatatcgt ccggatgaac ctgatcgtgc caccacgaaa ccgtcgcggg
     3421 cacctcgtca cgttcccagc cgaggatgtc gtagagacct cgcatgcgct cgacccgacc
     3481 cgtttccaga tcggtcgaat aaataaggcc cgtgatagct tcggccgtca gtaagaactg
     3541 ctgctcctgt gccaggagac gttgctcggc ctgcttgcgg tcagagatgt cgaggaccgt
     3601 acctgcgatc cgtgccgcga cacgatccga tccactgccg acgaagtgcg cccgcccgag
     3661 cgccgagacc caggcgacac tgccgtcggc acggcgtacg cgatggtcaa cgtcgagacg
     3721 cccctggccc gcagggtcga gcgcggcgcg ccagcacttc tcgacgccct cgaggtcctc
     3781 ctcaaggatg cggctgcgcg cggcctgtcg agtgacgacg gcgtctgcgc tgaagccata
     3841 gatttcccgg gcgcgggccg acagcgtgat tgaatccgtg gcgagatcga catcccagat
     3901 gccgaggcgg gtcgattcga ttgccagctg caggcttgtt tccaggcgga cgacctgcgc
     3961 tgcagtcgac ctctcacggc tctggtcgag cacggcgccg acgcactgca atccgtggga
     4021 gttcgagccg agaccatggc gctccgtcac caggccagca accagcaccg accgtgcctt
     4081 ggggccctgg ccgatcgcaa tctcggtgcg gaaccgctgg ccctcaccct gggcgatagc
     4141 gtcgtgcatg gcccggtgca cgcgatcggc ctcagtcggt gccagttggg caacgaactc
     4201 ggcgaggcgc cgtggcccgg gcatcgggat gccgaggatg ttgcgcgctc gctccgacca
     4261 gagcagttcg tccgtctctg catcccacac aaacgtaccg agttccgcgg ccgcgacaac
     4321 ggctttgagt ctttcctcgc tggcccgcag ggccgccgag gatcgcgcga catcctcgat
     4381 gtccgttgca ctgccgaacc agcgcaggct gccgtctgca cgacgcgagg gcgacgcccg
     4441 tatcagggtg gtccgaaagg ctccttcgag tgaccgcagt cgcagccgca gctcgagcgg
     4501 ctgcatcagc tggatggcct ggacgagttg ggtatcgaag gcctgaaggt cgtcgggatg
     4561 cacggccgag cgccatccgg aaccgaggct gagttcccgt ggaagcccgg tgaactcggt
     4621 ccagcgtgaa ttcaggtact cgaactggcc gtccgtggtg gcgacgaaga caatctccgg
     4681 caccgaagcg gccagcagtt cgaagctgtc ccggctttca gtcagcgaac gttcaatgcg
     4741 gtcacgagcc gcgacttcct cgtggagacg cttgaccacc ttcgtctctt cccggcgcgc
     4801 cttctgcaga gcggcgaaca cgaccgagat gacgataccg agcaaggcga gcaggacgat
     4861 gcgggtctga tcctgcggat cgacgacgag cagcgagccc gccggctcgt agacgtacgc
     4921 gaccagcgtc acacccagca acgtcgacac aacgccggca atcagaccgc cataggcagc
     4981 gctcagggct gaggcgagca gcagcacgag cagtggatgg cgtccctcgg ggatccggcc
     5041 cagcacgtac acggtgaacg cgaaggccag cagcgtcagc aggaccgctg ccatctgtgc
     5101 cttccaccac ttgaccttgg gtatcagcat tgtcccgcgg tagttcgaac cgaccgcagc
     5161 cgatgatgcc atcgaccgcc cggcacgggt tcaccgacag cgcgatcaac gccccatgtg
     5221 cgggacagag cgagagggaa agtgccggat tggcgagaca ggcagtcccg tcgcgctccg
     5281 gcgggcgcgt ggccgctcgt tcagtgaggt cgccggacga gcccgacgat gaacgagacg
     5341 accgcaatga tgatgaagag gaaaaaaatc cactgcgcga tcgacgacgc cccagcggcg
     5401 atgccaccaa aaccaaagag cgcggcgacg agtgcaatga cgaagaacac ggctgcccag
     5461 taaagcatgg agaccccctg taaacgtaga gttagtcgtg attcggactg aaacccatgt
     5521 ccggcgcgtg cgccgcacgg cgtttccgtc ccggcaaact ccatgcctgt gcggatcggt
     5581 tcgctttccg aatccacttc acttgacccg cggagatgtc ggccgcgtgg cacaaagttt
     5641 gcctcccccc gcgccatgga aacgacgaca ccgaaacccg cggccgcacc ggacgccgca
     5701 gtcacgccgt cccgatcgcg ggcgcgccac atcgaacgca cggcactcgt cgtgatcgcg
     5761 ctcatcgtga cgtgcgcggc gctggtcttc gcacgcaccc tgctgattcc catcgtggtc
     5821 gccgtcatgc tgtcgctgct gctgcggccg ctcgtcctgc tgctggcgcg ctacctgcgg
     5881 attcccgatg cgatcggttc gctggccatc gtggcactgg ggctgaccgg cgtcgtgtgg
     5941 ttcggaacgt atctttatga acctgcagag aagcttgtga aaatggggcc gggtgaagtg
     6001 cgccagctcg agcggaagct gcgcgatctg gcccggccgg tgcaggcaat ccgcggcgcc
     6061 accgacaagg tgaccaagct cgctgaggcc gcggatccga ccgacaagcg acgcgaagtc
     6121 gtcatgcagt cgggagggtt cctgcagtgg ttcgagac
//
LOCUS       DATIRT010000116         1784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_997779, whole genome shotgun sequence.
ACCESSION   DATIRT010000116 DATIRT010000000
VERSION     DATIRT010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997779"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /gene="hrcA"
                     /locus_tag="VLN25_02845"
     CDS             complement(<1..875)
                     /gene="hrcA"
                     /locus_tag="VLN25_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HSQ09542.1"
                     /translation="MLDERARTLLKALIEHYVVDGQPVGSRALSRLSGLELSPATIRN
                     VMADLEDLGYISSPHTSAGRIPTPRGYRFFVDTLLTLRPLGEQQASIIEGQLRGGDPQ
                     RAIQNAAQLLSSLSQFAGVVLTPRRSSSFKQLEFVRLSDRRVLLIIVTPEGDVQNRVL
                     MTQAAFTQSQLTEAANMLNEHFAGLSFNQARARLHTELRQLSEDISALMEAAVQAGSE
                     AEAEAESDPVVVSGERNLLGISDLATDVETLRRLFDLFEHKTRLIHLLDTSIGAQGVQ
                     IYIGGESQLVPMDEM"
     gene            960..>1784
                     /locus_tag="VLN25_02850"
     CDS             960..>1784
                     /locus_tag="VLN25_02850"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF002561.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HSQ09543.1"
                     /translation="MSQAFRNVAIFGKPRAENIREPLLAIARVVEAAGARVLFDLATA
                     EQVGLDGVERQTVHAIGKQADVAVVLGGDGTMLCIARELAPFRVPLIGINHGRLGFMT
                     DIALDQMAETLPAMLGGRYETDRRLVLDAEVRRAGQVAYHAMAVNDVVVSRGVTGGMV
                     EYTVRVDGVTMYNQRADCVILATPTGSTAYALSADGPILYPSLAGLLLVPVAPQTLSN
                     RPIVLPDDVVVEIEITDVRDASAHFDMQAYSSLVPGDIVRARRSHDLVTLLHPIGYN"
BASE COUNT          297 a          581 c          590 g          316 t
ORIGIN      
        1 ctcatctcgt ccatcggcac cagctgcgat tcgcctccga tgtagatctg cacgccctgc
       61 gcaccgatcg acgtgtcgag caggtggatc aggcgggtct tgtgctcgaa gagatcgaac
      121 aatcgccgca gcgtttcgac atcggtggcg agatcactga tgcccagtag gttgcgctcg
      181 ccggacacca cgaccggatc agattccgct tcggcttcgg cttcgctgcc ggcctgcacc
      241 gcggcctcca tcaatgccga gatgtcctcg ctgagttgcc gcagctcggt atgcagccgc
      301 gcgcgcgcct gattgaaact cagaccggcg aagtgctcgt tcagcatgtt cgcggcttcg
      361 gtcaactgcg actgtgtgaa cgccgcttgc gtcatcagca cgcggttctg aacgtcgcct
      421 tccggtgtga cgatgatcag cagtacacgc cggtccgaca ggcgcacgaa ctcgagctgc
      481 ttgaatgacg acgagcgacg cggcgtcagc accacgccgg caaactgcga cagactcgac
      541 agcagctgcg ccgcattctg tatcgcgcgc tgcggatcgc ccccgcgcag ctgcccttcg
      601 atgatgctgg cctgctgctc cccgagcggc ctgagcgtca gcagcgtgtc gacgaagaaa
      661 cgataaccgc gcggcgtcgg aatccggccg gccgacgtat gggggctcga gatgtagccg
      721 aggtcctcga ggtccgccat cacattgcgg atcgttgcag gcgacagctc caggcctgag
      781 aggcgagaca atgcgcgcga cccgaccggt tggccgtcga cgacgtagtg ctcgatcagg
      841 gccttgagca gggtccgggc gcgttcatcc agcatgggga aattctacgg tggttgcccc
      901 ggccgcaaag ggccgaacgg tcatccatag ggttggacag tgtcctgcat aatcgaatca
      961 tgtcccaggc ctttcggaat gtcgccatct tcggcaagcc ccgcgcggaa aacatccgcg
     1021 agccgctgct tgccatcgcg cgggttgtgg aggccgccgg cgcccgggtg ctgttcgacc
     1081 tggccacggc cgagcaggtg gggctcgacg gggtcgagcg ccagacggtc catgcgatcg
     1141 gcaagcaggc ggatgttgcg gtcgttcttg gcggtgacgg cacgatgctt tgcattgcgc
     1201 gcgaactggc accgttccgg gtgccgctga tcggcatcaa tcatggccgc ctcgggttca
     1261 tgaccgacat cgcactggac cagatggccg agacactgcc ggcgatgctg ggcggccgct
     1321 atgaaaccga tcgccggctc gtgctcgatg ccgaagtgcg ccgtgcgggg caggtggcct
     1381 accacgcgat ggcggtcaac gacgtggtcg tgtcgcgcgg cgtgaccggc ggcatggtcg
     1441 agtacacggt tcgggtcgat ggcgtcacga tgtacaacca gcgcgccgac tgcgtgatcc
     1501 tggccacgcc gaccgggtcg accgcctacg cgctgtcggc cgatggcccg atcctgtatc
     1561 ccagtctggc cggcctgctg ctggtgccag tcgcgccgca gacgctgtcg aaccggccga
     1621 tcgtgctgcc ggatgacgtc gtggtcgaga tcgagatcac cgacgtgcgc gacgccagcg
     1681 cgcacttcga catgcaggcc tactcgagcc tggtcccggg cgacatcgtg cgcgctcgcc
     1741 gcagccacga cctggtcacg ctgttgcatc cgatcggcta caac
//
LOCUS       DATIRT010000117         2665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1391257, whole genome shotgun sequence.
ACCESSION   DATIRT010000117 DATIRT010000000
VERSION     DATIRT010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391257"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..616)
                     /locus_tag="VLN25_02855"
     CDS             complement(152..616)
                     /locus_tag="VLN25_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSQ09544.1"
                     /translation="MAKVTTPPAGYHSVTPYLAIRDAVAAVEFYRRAFGAELVMKLTM
                     PDGKYAHAEIKIGDSHIMMAEENPEWGNQSPALLGGSPVSFMIYVPDVDAAFARALAA
                     GCTQVRPVEDQFYGDRTGTLKDPYGFQWSIGTHMEDVSEAEGQKRMEAMFSK"
     gene            complement(663..1457)
                     /locus_tag="VLN25_02860"
     CDS             complement(663..1457)
                     /locus_tag="VLN25_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HSQ09545.1"
                     /translation="MESMHYHRPASVADAVKLVGQHGDDKILAGGQSTLPSMRLGLLA
                     PTGWIDLAGVAELQGIRVDGNKVVVGAMSTHAAVAASKDVQSRIPALAQLAGGIGDRQ
                     VRNRGTIGGSLANNDPAACYPAAVLGLGATVQTDRRMIAADEFFKGLYETALAAGEII
                     TAVHFPVPEKAAYLKFKQPASRFALVGVFVAKTSGGVRVAVTGAGSVVFRVKALEDKL
                     TAKFAPESCDGVSVPASGLNSDLHASAEYRAHLITVLAKRAVAAAA"
     gene            complement(1477..>2665)
                     /locus_tag="VLN25_02865"
     CDS             complement(1477..>2665)
                     /locus_tag="VLN25_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HSQ09546.1"
                     /translation="KIDPAELRRRNFIKTFPYATPVGLSYDTGDYNATLTRAIELADV
                     KGFAARKAESANKGLLRGLGYSCYIEACGLAPSNIAGALGARAGLFEAGEVRVHPTGK
                     VTVFTGSHSHGQGHETTFAQVVADRLGIPLDDVDIQHGDTGKILFGMGTYGSRSIAVG
                     GTAIVKAIDKVVAKGKKIAAHLLEASDADIEFANGEFRVAGTDRKKAFAEVSLAAYVP
                     HNYPLDKLEPGLNENAFYDPTNFTYPAGSYVCEVEVDPETGVVRIDRFVACDDFGNVI
                     NPMIVEGQVHGGLAQGIGQALLEHGIYNEDGQLVTGSYMDYAMPRADDLPKFTVETAK
                     GTPCTHNPLGVKGCGEAGAIGSPAAVINALTDALAHLGVRDVPMPATPHIVWKTIRTA
                     TRV"
BASE COUNT          418 a          903 c          864 g          480 t
ORIGIN      
        1 acaaatcgat ccgccgcgct gccgcttcca cctgctcttg cgcaagctgc cgcgccgcga
       61 gcgcatcgaa cggcagaacc actggctcga actcgccgtt cgaggtggta tcgagcttga
      121 ttggaagctg cactcggtca gacggacaga actacttgct gaacatcgct tccatgcgct
      181 tctgtccctc ggcctcgctc acgtcttcca tgtgcgtgcc gatcgaccac tggaaaccgt
      241 acgggtcctt cagcgttccg gtgcgatcgc cgtaaaactg atcctccacc ggccgcacct
      301 gcgtgcaccc ggccgccaat gcgcgcgcga acgccgcatc gacgtcgggc acgtagatca
      361 tgaaggacac cggcgagccg ccgagcaggg ccgggctctg gttgccccac tccgggttct
      421 cctcggccat catgatgtgc gagtcgccga tcttgatctc ggcgtgggcg tacttgccgt
      481 caggcatcgt cagcttcatc accagctcgg cgccgaacgc gcgcctgtag aactcgacgg
      541 ccgccaccgc gtcgcggatc gcgaggtacg gcgtcaccga gtggtatccc gcgggcgggg
      601 tcgtgacctt ggccatggct gcctccgtgc cagttgaacc gtggatcgat gaacgcgcga
      661 ggtcaggctg ccgctgccac tgcccgcttg gccagcaccg tgatcaggtg cgcgcggtac
      721 tcggccgacg cgtgcaggtc ggagttcagt cccgaggccg gcacgctgac gccgtcgcag
      781 gactccggcg caaacttggc ggtcagtttg tcttcgagtg ccttcacccg gaacacgacg
      841 gacccggcac cggtcactgc gacgcgcacg ccgcccgatg tcttcgcgac gaacacgccg
      901 accagcgcga agcgcgaagc gggctgcttg aacttcaggt aggccgcctt ctccggcacc
      961 ggaaagtgca cggccgtgat gatttcgcca gcggccagcg cggtttcgta caggcctttg
     1021 aagaattcat cggccgcaat cattcgccgg tccgtctgca cggtcgcgcc gagcccgagc
     1081 acggcggccg ggtagcaggc ggccgggtcg ttgttcgcga gcgaaccgcc gatcgtgccg
     1141 cggttgcgca cctggcgatc gccgatgccg ccggcgagtt gggccaacgc cgggatgcgt
     1201 gactgcacat ccttcgacgc ggccactgcc gcgtgtgtcg acattgcgcc gacgacgacc
     1261 ttgttgccgt cgacccgaat accctgcagc tcggcgacgc cggcaagatc gatccagccc
     1321 gtcggtgcca gcaggccgag ccgcatcgat ggcaacgtcg actggcctcc ggcgaggatc
     1381 ttgtcgtcgc catgctggcc gaccagcttg acagcgtcgg ccaccgaggc ggggcggtga
     1441 tagtgcatcg attccatcat catctcctca gtgtcgttac acgcgggtcg ccgtgcggat
     1501 cgtcttccac acgatgtgcg gcgtcgcggg catcggcacg tccctgacgc cgaggtgcgc
     1561 gagcgcatcg gtgagcgcat tgatgactgc cgccggcgag ccaatcgcac ccgcctcgcc
     1621 gcatcccttg acgcccaacg ggttgtgcgt gcacggcgtg cccttggcgg tctcgaccgt
     1681 gaacttcggc aggtcgtcgg ctcgcggcat cgcgtagtcc atgtagctgc cagtgacgag
     1741 ctggccgtcc tcgttgtaga tgccgtgctc gagcagcgcc tgaccgatgc cctgggcgag
     1801 cccgccgtgc acctgcccct cgacgatcat cggattgatg acgttgccga agtcgtcgca
     1861 ggccacgaag cggtcgatac gcacgacgcc ggtttcggga tcgacctcga cctcgcagac
     1921 ataggagccc gccggatacg tgaaatttgt cggatcgtag aacgcgtttt cattgagtcc
     1981 cggctcgagc ttgtcgagcg ggtagttgtg cggcacgtag gcggccagcg acacttcggc
     2041 gaaagctttc ttgcggtcgg tgccggcgac acggaactcg ccattcgcga actcgatatc
     2101 ggcgtcactc gcttccagca ggtgcgcggc aatcttcttg ccctttgcga ccaccttgtc
     2161 gatcgccttc acgatggcgg tgccgccgac cgcgatcgag cgcgagccgt aggtgcccat
     2221 gccgaacagg atcttgccgg tgtcgccgtg ctggatgtcg acatcgtcga gcggaatgcc
     2281 gagccggtcg gccaccacct gcgcgaacgt ggtctcgtgg ccctggccgt gcgaatgcga
     2341 accggtgaac accgtcacct tgccggtcgg atgcacccgc acttcgccgg cttcgaaaag
     2401 acccgcgcgc gcaccgagcg cgccggcgat gttcgacggc gccagcccgc acgcttcgat
     2461 gtagcaggag tagccgaggc cgcgcagcag ccccttgttc gcgctctcgg ccttgcgggc
     2521 ggcaaagccc ttgacgtccg cgagttcgat cgcccgggtc agcgtggcgt tgtagtcgcc
     2581 ggtgtcgtag ctcagcccga cgggcgtggc gtacgggaag gtcttgatga agttacgccg
     2641 gcgcagttcg gccggatcga tcttc
//
LOCUS       DATIRT010000118         5504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_408058, whole genome shotgun sequence.
ACCESSION   DATIRT010000118 DATIRT010000000
VERSION     DATIRT010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408058"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..584)
                     /locus_tag="VLN25_02870"
     CDS             complement(81..584)
                     /locus_tag="VLN25_02870"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HSQ09547.1"
                     /translation="MTAIDWALVALLGLSVLLGLVRGVVRELFALSGWIVGILLALRF
                     AEPLGTVLPLDLPLGARAALAGIFIVVAILLLAALVAALLRAMLTAAKLSLEDRLLGA
                     TFGLARGVIVIGLAVLVAVAAGAPRQSWWQASLLLPWVQASVGFASPLLPQSLARYAS
                     KPGPQGN"
     gene            complement(591..1340)
                     /locus_tag="VLN25_02875"
     CDS             complement(591..1340)
                     /locus_tag="VLN25_02875"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HSQ09548.1"
                     /translation="MSLAFWRRKNTAPDASELRSTRTAASDDDSGARTDPTAVLRARA
                     RRRLIGAAALLLLVVIVVPMVLDPEPKPVPDNIPIDIPSEKTKFSPRLALPPVPAQES
                     VSVAPPPDAAPTKAAPPAPAAAPTAATPPPGAEPAKDKPAKSVEASKAEERRAREVLD
                     GKAGTEKSPVPVKGGKFAVQAAATSSEAAALELSERLKKAGLPPYTERIDTAEGARYR
                     VRVGPYANREEAERVRGRLKALGINANIVSS"
     gene            complement(1361..2659)
                     /gene="folC"
                     /locus_tag="VLN25_02880"
     CDS             complement(1361..2659)
                     /gene="folC"
                     /locus_tag="VLN25_02880"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HSQ09549.1"
                     /translation="MLLEKLSLVDWLTRLERLHPKGIDLGLDRVRVVADRLELASSAS
                     TIVVGGTNGKGSTCAMLDSILRAAGYRTGLYTSPHLLKFNERARIDGVDAGDEQLIES
                     FEAVEAVRGTTSLTYFEFTTLAILRLFAQARLDAVVLEVGLGGRLDAVNLVDADVAIV
                     TSVDIDHIDYLGSTRELIGLEKAHIYRTGHVAICSDPRPPQSLLDYADRIGADLWRIG
                     HDFNYQGDRQQWAYGGRRVRRSGLPYPALRGANQLLNASGALAALEALAERLPVSQQA
                     VRHGLLTVELPARFQVLPGRPATILDVAHNPHAAAVLAENLDSMGYFPVTHGVFGMLG
                     DKDIAGVVGKLGDRIDHWHVGPTPGSRGSSAAQTAAQLRAVGVGMASGQSITEHATLA
                     DAYAAARDSASADDRILAFGSFLTVADVMRAIRTHVRSDP"
     gene            complement(2697..3569)
                     /gene="accD"
                     /locus_tag="VLN25_02885"
     CDS             complement(2697..3569)
                     /gene="accD"
                     /locus_tag="VLN25_02885"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HSQ09550.1"
                     /translation="MSWIDKILPPRIKREEGSARKVVPEGLWTKCPGCEAVLYSAELE
                     KTQMVCPKCAHHLRIGARQRLDALMDAEGRYEIGQEVLPVDALKFKDSRKYTERLQEA
                     MEETGETDALVVMGGSIRAVPVVVACFEFAFMGGSMGSVVGERFVRGVGTAIEQKVPY
                     ICITSTGGARMQEGLLSLFQMAKTTAMIARLAEAKLPFISVLADPTMGGVSASFAFMG
                     DVVIAEPKALIGFAGPRVIEQTVREKLPEGFQRAEFLLTKGAIDMIIDRREMRDEIAR
                     LLALLLKQPADAIS"
     gene            complement(3598..4416)
                     /gene="trpA"
                     /locus_tag="VLN25_02890"
     CDS             complement(3598..4416)
                     /gene="trpA"
                     /locus_tag="VLN25_02890"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012733649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HSQ09551.1"
                     /translation="MSRIGQRFAKLKAAGRKGLIPYIAAGDPEPSLTVPLLHALARAG
                     ADVIELGVPFSDPMADGPVIQRATERAIRHGVGLRQTLAFVAEFRTSDAVTPVVLMGY
                     ANPIERMGRHEFAEAARAAGVDGVLVVDYPPEECAEFAAEMRAAGIDPIFLLAPTSTD
                     QRIEEVARHAAGYIYYVSLKGITGAGHLDVASVAAKLPLIRKHSNVPVGVGFGIRDAA
                     SAVAVAQVADAVVIGSRLIQEMEATPRERAVEAVEALVREIRSALDREFVRAAA"
     gene            complement(4416..>5504)
                     /gene="trpB"
                     /locus_tag="VLN25_02895"
     CDS             complement(4416..>5504)
                     /gene="trpB"
                     /locus_tag="VLN25_02895"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HSQ09552.1"
                     /translation="RPSPIYHAKRWSELLGGAQIYLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKPRVIAETGAGQHGVATATVAARYGMKCVVYMGSEDVGRQAANVYRMKLLGAE
                     VVPVESGSRTLKDALNEAMRDWVTNVENTFYIIGTVAGPHPYPMMVRDFNSVVGKECL
                     WQMPEMVARQPDVIIACVGGGSNAMGIFYDYIPHEQVKLVGVEAAGEGLTTMRHAASL
                     QCGTPGVLHGNRTYLLQDENGQITETHSVSAGLDYPGVGPEHAWLKDSGRAVYVGATD
                     EEALRAFHECCRIEGIIPALESSHALAYATKLAPTLPKDKVLLVNLSGRGDKDLHIVA
                     EAGALEFYCRASCREATFEYRQRHAGER"
BASE COUNT          960 a         1903 c         1718 g          923 t
ORIGIN      
        1 agagcccgcc ctgtcggtcg aaggcgcgta attgcctcct caggagggag ccagcaaagc
       61 aggtgtttgc gcaattcatc ctagtttccc tgcggccccg gcttcgacgc atagcgtgcc
      121 agggactgcg gcaacagcgg cgaggcgaat cctacgctcg cctgcaccca cggcaacagt
      181 aaagacgcct gccaccacga ctgacgcggt gcgcccgccg ccacggcaac gagcacggca
      241 agcccaatca cgattacgcc acgcgccaaa ccgaaggtgg cgccgagcag ccgatcttca
      301 agcgacagct tggccgccgt cagcatcgcg cgcagcagcg cggccacgag tgcggccagc
      361 aggagtatgg caacgacgat gaagatcccg gcaagggccg cccgcgcacc gagcggcagg
      421 tcgagcggga gcaccgtgcc cagtggttcg gcaaaccgca gcgcgagcag gatgccgaca
      481 atccatccgc tcagcgcgaa aagttcgcgg acgacgccgc gcaccagtcc cagcaacacc
      541 gacaggccga gtagtgcgac gagggcccag tcgatggcag tcatcggtcg ctaggaactg
      601 acaatgttgg cattgatacc gagcgccttc agccgaccac gcacgcgctc ggcctcttcc
      661 cgattggcgt acggtccgac ccggactcga tagcgcgccc cctctgcggt gtcgatccgc
      721 tcggtgtacg gcggcagacc ggccttcttc aggcgctccg aaagctccaa tgccgccgct
      781 tcgctcgagg tcgccgctgc ctgcaccgca aactttccac ctttcaccgg taccggactt
      841 ttttcggtgc ccgccttgcc atcaagcacc tctcgggcac gtcgctcttc ggccttggac
      901 gcttcaacgc tcttcgcggg cttgtccttc gccggttcgg cacccggcgg cggtgtagca
      961 gcggtcggcg cagcggcagg agctggcgga gccgcctttg tgggcgccgc atccggtgga
     1021 ggcgctacag acacgctctc ctgcgcagga accggaggca gcgcgaggcg cggcgagaac
     1081 ttcgttttct cgctgggaat gtcgatcgga atgttgtcgg gaaccggctt gggttcggga
     1141 tcgagcacca tcggcaccac gatcacgacc agcaggagca gcgccgctgc accgatgagc
     1201 cgccgccgcg ctcgcgcacg cagcaccgcg gtgggatcgg tgcgcgcgcc ggagtcatcg
     1261 tcggaggctg ccgtgcgggt ggagcgaagc tcggaggcgt caggggccgt gttcttgcga
     1321 cgccagaaag ccagggacat ccgtgctcca gtcgtgatcc tcagggatcc gaacggacat
     1381 gcgtgcgaat tgcacgcatc acgtcggcca cggtgaggaa ggaaccgaag gccagaattc
     1441 tatcatcggc cgatgcgctg tcccgtgcag cggcatacgc atccgcgagc gtggcgtgct
     1501 cggtgatcga ctgcccggat gccatgccaa caccgaccgc acgcagttga gccgcggtct
     1561 gcgctgcgct cgagccgcgg gatccgggcg tgggtcccac atgccagtgg tcgatccggt
     1621 cgccgagctt gccgacaacg cccgcgatgt ccttgtcgcc cagcatcccg aacacgccgt
     1681 gcgttaccgg aaagtaaccc atgctgtcga gattttctgc aagcacggcc gccgcatgag
     1741 gattgtgtgc cacgtcgagg atcgtggccg gacgccccgg cagaacctgg aagcgggccg
     1801 gaagctccac cgtgagcaat ccgtgtcgga ccgcctgctg cgagacgggt aggcgctcgg
     1861 cgagcgcttc gagcgcagcc agagcaccgg atgcattcag caactggttc gcacctcgca
     1921 gcgccggata gggcagcccg ctgcggcgga cgcggcgccc gccgtaggcc cattgctgcc
     1981 ggtcgccctg gtagttgaag tcgtgcccaa tgcgccagag gtcggcgccg atgcgatcgg
     2041 cgtaatcgag cagcgactgc ggcggcctgg gatcactgca gatggcgacg tgacccgtgc
     2101 ggtagatgtg ggctttctcc agtccgatca actcgcgcgt cgaaccgagg tagtcgatgt
     2161 gatcgatgtc cacggatgtg acaatcgcca cgtcggcatc gacgaggttc acggcgtcga
     2221 ggcggccgcc cagaccgacc tcgagcacta ctgcatccag ccgggcctgt gcgaagagac
     2281 gcaggatggc cagcgtggtg aactcgaagt aggtgagtga cgtcgtgccg cggactgcct
     2341 cgaccgcctc gaagctttcg atcagctgct cgtcaccggc atcgacaccg tcgatacgcg
     2401 cccgttcgtt gaacttcagc aggtggggtg aggtgtagag cccggtgcgg tagcccgcgg
     2461 cgcgcaggat gctgtccagc atcgcgcacg tggagccctt gccgttcgtg ccgccgacaa
     2521 cgatggtcga cgccgaggat gcgagctcga gccgatcggc gacgacgcgc acccggtcca
     2581 ggccgaggtc aatgcccttc gggtgcagac gttcgaggcg agtaagccag tcgaccagcg
     2641 agagtttttc caagagcatc aatcgccgga cgcctggtgg ccggcactcc tgaacactag
     2701 ctgatcgcgt cggccggctg cttcagcagc agcgcgagca ggcgcgcaat ctcgtcgcgc
     2761 atctcgcgcc ggtcgatgat catgtcgatg gcacccttgg tcagcagaaa ttccgcacgc
     2821 tggaagccct cgggaagttt ctcgcgcacg gtctgttcga tgacacgcgg gccggcgaag
     2881 ccaatcagcg ccttcggctc ggcgatcacc acgtcgccca tgaatgcgaa gctggcggac
     2941 acaccgccca tggtcggatc ggccagcacc gagatgaacg gcagctttgc ttcggccagg
     3001 cgcgcaatca tcgccgtcgt cttggccatc tgaaacagcg acagcaaccc ttcctgcatg
     3061 cgcgcaccgc cggtggatgt gatgcagatg tacggcacct tctgttcgat ggccgtcccg
     3121 accccgcgca caaatcgctc tccaaccacc gaccccatcg aaccgcccat gaaggcgaac
     3181 tcgaagcacg ccaccacgac cggcaccgcg cggatcgagc ctcccatgac gacgagggca
     3241 tcggtctctc cggtttcctc catcgcctcc tgcaggcgct cggtgtactt gcggctgtcc
     3301 ttgaacttca gcgcgtcgac cggcaggacc tcctgcccga tctcgtagcg cccctcggca
     3361 tccatcagcg catcgagccg ctggcgtgca ccgatgcgta ggtggtgtgc gcacttcggg
     3421 cacaccatct gagtcttctc gagctctgcg ctgtacaaca ccgcttcgca gcccgggcac
     3481 ttggtccaca ggccctcagg aacgaccttt ctggccgagc cctcttcccg cttgatgcgc
     3541 ggcggaagaa tcttgtcgat ccaactcatc gtggtactcc ggtctgtcgc tctaatctca
     3601 agctgccgcc ctgacgaact cgcggtccag cgcggaacga atctcccgca ccagcgcttc
     3661 cacggcctcc accgcacgtt cgcgcggtgt cgcttccatt tcctgaatca accgcgagcc
     3721 gatcaccacg gcatccgcga cctgcgccac cgcgactgcg ctggccgcat cacggatgcc
     3781 gaagccgacg ccgaccggca cgttcgagtg cttccggatc agcggcagtt tcgcggccac
     3841 gcttgcgaca tccaggtgac cggccccggt gatccccttc aggctgacgt agtagatgta
     3901 gccggccgcg tggcgcgcga cttcctcgat gcgctggtcg gtcgacgtcg gcgcgagcag
     3961 gaagatcgga tcgatgccgg ccgcacgcat ctcggctgcg aactccgcac actcctccgg
     4021 cgggtagtcg actacaagca caccgtccac gccggccgcg cgcgccgcct cggcaaactc
     4081 atgccgaccc atgcgttcaa tcgggttggc ataacccatc aaaactacgg gggtcaccgc
     4141 atcggacgta cgaaactccg cgacgaacgc cagtgtctgg cgcaagccga caccgtgccg
     4201 aatcgcccgc tcggtcgcgc gctggatcac cgggccgtcg gccatcggat ccgagaatgg
     4261 aacgccgagc tcgataacat ccgccccggc gcgcgccagc gcatgcagca gcggaaccgt
     4321 cagcgacggc tccgggtcgc cggcggcgat gtacggaata aggcctttgc ggccggccgc
     4381 ttttagcttg gcaaagcgct ggccgatgcg cgacatcagc gctccccggc gtgccgctgc
     4441 cgatactcga acgtcgcctc gcggcacgac gcgcggcagt agaactccag cgcgcccgcc
     4501 tcggcgacga tgtgcaggtc cttgtcgcca cgtcccgaca ggttcaccag cagcaccttg
     4561 tccttcggca gtgtcggcgc cagcttcgtc gcatatgcga gcgcatggct cgattccagc
     4621 gcggggatga tgccctcgat acggcagcac tcatggaagg ccctgagtgc ctcttcgtca
     4681 gtggcaccca catacacggc gcggccgctg tccttcagcc aggcatgctc gggacccacg
     4741 cccggatagt cgagacccgc cgacaccgag tgcgtctcgg tgatctgacc gttctcatcc
     4801 tgcagcaggt aggtacggtt gccatggagc acgccgggcg tgccgcactg caggctcgcc
     4861 gcatgacgca tcgtcgtcag accttcgccc gcggcttcga caccaacaag cttcacctgt
     4921 tcgtgcggaa tgtagtcgta gaagatgccc atcgcgttgc tgccgccacc gacgcaggca
     4981 atgatgacgt caggctggcg tgccaccatc tcgggcatct gccacaggca ctccttgccg
     5041 accaccgagt tgaagtcgcg caccatcatc gggtacggat gcggtccagc caccgtgccg
     5101 atgatgtaaa aagtgttctc gacgttcgtc acccagtcgc gcatcgcctc gttcagcgcg
     5161 tccttcagcg tgcgcgatcc gctttccacc ggcaccacct cggcgcccag cagcttcatg
     5221 cggtacacgt tggccgcctg gcgtccgacg tcctcgctgc ccatgtacac gacacatttc
     5281 atgccgtaac gcgcggccac ggtggcggtc gccacgccgt gctgccccgc accggtctcc
     5341 gcaatcacac ggggcttgcc catccgcttc gccagcaggg cctggccgat ggtgttgttg
     5401 accttatgtg ccccggtgtg attcaggtct tcgcgcttca ggtagatctg ggcaccgccc
     5461 agcaactcgc tccagcgctt tgcgtgatag atcgggcttg gacg
//
LOCUS       DATIRT010000119         6524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_505885, whole genome shotgun sequence.
ACCESSION   DATIRT010000119 DATIRT010000000
VERSION     DATIRT010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6524
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505885"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VLN25_02900"
     CDS             <1..632
                     /locus_tag="VLN25_02900"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HSQ09553.1"
                     /translation="GFLQWFETAQLVTASVVSCVILLYFLLASGDLFLRKLVRVLPGL
                     RDRIVAVDISRSVQGEIGRYFASTTLINAGLGVCTALTMMGLDMPSPALIGTVAAVLN
                     FVPYIGSLVTLAGILVTATVTFDTAWQIALPPLAFLALSAIEGQIVQPLVLGRSLALN
                     PVVLFVWMMVWGWLWGIAGFLVAVPMLVALRICADRFESLAMLSEFIRR"
     gene            complement(619..2790)
                     /locus_tag="VLN25_02905"
     CDS             complement(619..2790)
                     /locus_tag="VLN25_02905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HSQ09554.1"
                     /translation="MTSAGPPEPRRLLLYTAFGLAGLVALLAVTPLGPALFSQNGFLP
                     HGYCFTWSPSLLTLHLAADLLIAAAYFSIPLTLAYLVSKRADLPFNWVFLLFAAFIVA
                     CGVTHLVGVLTLWYPFYWFAGAVKAVTALVSVTAAVAVVLLMPRLLALPSAAQLRSAN
                     AQLEAEVTERRLKDAELQLIKQDLEQRVAARTAELATTNALLAQQREWLHTILTSICD
                     AVIATDAQGRVVFLNPVAEQLTSWRDADAQGQPLERIWRVRGSDELGAAPLVAEVLRR
                     GRVVRGGPATVEFGNEGFRRVLEVTAAPIAPAGNAARGVVIVVRDLTAQRDAERSSVE
                     LSSSLASTNALLDTLFERAPIGMGFWDRELRYVRVNEALARINGLPVEAHLGRTINEV
                     LPKLEPRVEAAFRTVLETGQPLSELELQGETPAAPGVLRTWRTSVYPVTVGSALVGVG
                     AVCDEITDLKRVEAQRLELLEAESHARARAEEMNRLKDDFISTLSHELRNPLNSIVGW
                     THLLESGKLSEADREKAIDRITRNVKTQAKLVEELLDFSRLNSGKLQLKLERLNASEL
                     ARTACETFRTGAQAKNIELDCRIDLPELTVLADAERLRQVLSNLLSNAVKFTPEGGSV
                     VAHVRRNGPTIEFEVRDTGAGIDAEFLPHVFDPFRQASLGARQPLQGVGIGLTIVKRL
                     VELHGGRVTARSDGPGKGASFLVELPVASNDGTQTTLTSVE"
     gene            complement(3264..4643)
                     /locus_tag="VLN25_02910"
     CDS             complement(3264..4643)
                     /locus_tag="VLN25_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSQ09555.1"
                     /translation="MTHALIVDDDIDSAEMLATLLATEGLTVAVAHDLAGARRQLALQ
                     TPQLILLDLQLPDGSGMSLLDDEKVRVESEIVLVTGNASLETSIHALRMGAADYLIKP
                     VNIKQLKGILSRLSSPATIDSEIETLKSEAEGTARFGHLRGRSAAMLRIYAQIGRVAP
                     TAVSVLITGESGTGKEVVARTLHDLSRRRERPSLAVNCGAISPNLIESELFGHEKGAF
                     TGASRQHFGYFERAHGGTLFLDEITEMPSELQVKLLRVLETGTFMRVGSLETLHADVR
                     LVAATNRDPLLAVAEGKLREDLLYRLNVFPIHLPPLRERLEDVPLLAQHFLEEIIEKE
                     GEPKTLSSAVLRRFESYAWPGNVRELRNAVYRCYVMAADSEITEDWLPEGAVKPSRSG
                     PDATSVRVELGTPLADIERKVILATLKHCDGQKEQTAAALGISLKTLYNRLKEYSAMA
                     EADDEKVGR"
     gene            5044..5295
                     /locus_tag="VLN25_02915"
     CDS             5044..5295
                     /locus_tag="VLN25_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09556.1"
                     /translation="MSGPVTIALIVVLGIFAIPWLLGRPHGRSLVAIATLLLAALFMA
                     FEAGNEAATVTRIVLAGLLGFAPAVAGIIVARLQRRAPP"
     gene            5421..5618
                     /locus_tag="VLN25_02920"
     CDS             5421..5618
                     /locus_tag="VLN25_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09557.1"
                     /translation="MLPWQELALKIAVTALLLAVVLPLVGFAFTRDGSSGLRALRTGA
                     IILGVIALVAGVAALLGEVWR"
     gene            complement(5696..>6524)
                     /locus_tag="VLN25_02925"
     CDS             complement(5696..>6524)
                     /locus_tag="VLN25_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09558.1"
                     /translation="AAASIATDIGTVRPPGHRDFALDEAQRGLVESLRRAATDGMRAQ
                     LLVLSFLVDKDRELRGQQRALIAEAYGGKAAQIADGYHEQVQRLPPGARQPLVDVAMP
                     ALRKLPDDAREALLRTAHLLIAADGRLTVREFLLFTILKRRLGRDAGHPVPVNYSAIA
                     AVAREAGVVLSLVAMVRLPERPEHAFNAGAVQLREAELPFVAASSLRLDSVSDALDRL
                     NQLAPLAKPQLIKAATAVAFVDGQTNWKAASSLRMICTALDAPLPPQVQQVEAGVSG"
BASE COUNT         1098 a         2164 c         2090 g         1172 t
ORIGIN      
        1 gagggttcct gcagtggttc gagacagcgc agctggtcac ggcctccgtt gtctcgtgcg
       61 tcatcctgct ttacttcctg ctggcgtcgg gcgatctgtt tctgcgcaag ctggtccgcg
      121 tgctgcccgg attgcgggac cgcattgtcg cggtcgacat ctcgcgttcg gtccagggcg
      181 agatcggacg ttacttcgcg tcgaccacgc tgatcaacgc cggcctgggt gtgtgcaccg
      241 cattgacaat gatggggctc gacatgccat cgcccgcctt gatcggcacg gttgcggctg
      301 ttctgaattt cgtcccctac atcggctctc tcgtgacact cgcaggcatc ctggtcacgg
      361 ccacggtgac cttcgatact gcctggcaga tcgcattgcc gccgctggcc ttcctggcgc
      421 tatccgcaat cgaaggccag atcgtgcaac cgcttgtact gggccgcagc ctggcgctca
      481 atccggtggt gctctttgtc tggatgatgg tgtggggctg gctctggggc atcgccggat
      541 tcctggtcgc cgtcccgatg ctggtggcgc tgcgtatctg tgctgaccgt ttcgagtcgc
      601 tcgcgatgct gtcggaattc attcgacgct agtcagcgtc gtctgcgtgc cgtcattcga
      661 agccacgggc agttccacca ggaaacttgc gcccttgccc ggtccgtcgc tgcgtgccgt
      721 tacgcggcca ccgtgcagtt caaccagccg cttgacaatg gtcagtccga tgccaacccc
      781 ttgcagcggc tgacgcgcac ccaatgacgc ctgccggaac gggtcgaaca cgtgaggcag
      841 gaactcggca tcgatgccgg cacccgtgtc acggacttcg aactcgatcg tcggaccgtt
      901 gcgccgcacg tgcgccacga ccgaaccgcc ctcgggcgtg aacttcacgg cattcgagag
      961 caggttggaa aggacctggc gcaggcgctc ggcatcggcg agcacggtca actcgggcag
     1021 atcaatgcgg cagtcgagct cgatgttctt ggcttgggcg cccgtgcgga acgtctcaca
     1081 cgccgtccgc gccagctccg atgcattgag ccgctcaagc ttgagctgca gcttgccgct
     1141 gttcaggcgc gagaagtcga gcagttcctc gaccagtttc gcctgcgtct tgacgttgcg
     1201 ggtgatgcga tcgatcgcct tctcgcgatc ggcctcggat agcttgccgc tctcaagcag
     1261 gtgagtccag ccgacgatgg agttcagtgg attgcgcaac tcgtgggaca gtgtcgagat
     1321 gaagtcgtcc ttgaggcggt tcatctcttc cgcgcgggca cgcgcgtgcg actcggcctc
     1381 cagcaactcc aggcgctgcg cttcgacccg cttcaggtca gtgatttcgt cgcagacggc
     1441 gccgaccccg accagcgcgc tccccacagt caccgggtac acactggtgc gccaagtgcg
     1501 caatacgccc ggcgcggcgg gtgtctcgcc ctgcagttcc agctcgctca gcggctgccc
     1561 ggtttcgagc actgtccgga aggcggcctc gacgcggggt tcgagcttcg gcaggacttc
     1621 gttgatcgtc cgccccaggt gagcctcgac cggcagcccg ttgatacggg ccagggcttc
     1681 gttcacgcgc acatagcgca gctcccggtc ccagaacccc atgccgatcg gagcccgctc
     1741 gaacagcgtg tcgagcaacg cattggttga agcaaggctg gagctgagct cgacactgga
     1801 acgctcggca tcccgctgcg ccgtgaggtc gcgcacgacg atgacgacgc cacgagcggc
     1861 attgcccgcc ggcgcgatcg gggcggccgt cacttcgaga acgcgccgga atccttcgtt
     1921 gccgaactcg acggtcgccg gtccaccgcg aacgacgcgg ccgcggcgca gtacttcggc
     1981 caccaggggc gctgccccca actcgtcact accgcgcacg cgccagatcc gctctagcgg
     2041 ctggccctgc gcgtcggcat cgcgccacga agtcagctgt tcggccaccg gattcaggaa
     2101 caccacgcgc ccctgggcgt cggtggcgat caccgcatca cagatgctcg tcaggatcgt
     2161 gtgcagccat tcgcgttgct gcgccagcag ggcgttggtc gtcgccagtt cagccgtacg
     2221 tgccgcgaca cgctgctcga gatcctgctt gatgagttga agttccgcgt ccttgaggcg
     2281 ccgttccgtg acttccgcct ccagttgcgc attggcggag cgcagttgcg ccgcactcgg
     2341 caacgcaagc aaccggggca tcaggaggac gaccgccacc gccgccgtga cggacaccag
     2401 tgcagtcacc gccttgaccg cgccagcgaa ccagtaaaac gggtaccaca gggtgagcac
     2461 accgacgaga tgcgtcacac cgcaggccac gatgaaggcc gcgaacagca ggaagaccca
     2521 gttgaagggc aggtccgcgc gcttgctgac caggtaggcg agggtcagcg gtatcgagaa
     2581 gtacgccgcg gcgatcagca ggtcggcggc caggtgcagc gtgagcagcg acggtgacca
     2641 ggtgaaacag tagccgtgcg gcagaaaccc gttctgtgaa aacagagcgg gacccagcgg
     2701 cgtcaccgcc agcagggcaa cgaggccggc gaggccgaag gcggtgtaca gcagcagtcg
     2761 tcggggctcg ggcggtccgg ccgaagtcat tcggagatcc gtgacggatg gaaacggcgt
     2821 ctcgcgcggc cggccttggg agacagacac cgagtcgttg agcttcggta atgcaaattt
     2881 cctgccacgt ggctccacga acctgcagcg gccactcgat gagatgaacg gcgtctgcaa
     2941 atgcaaagtt cgactgcttt caaccgcttc ttacgtcttg aagccgacct cggctccatg
     3001 gtgtcccgcc gctcaatatc agcttgtaag gtttgccgca attgttgccg gcgcggcaca
     3061 gacaactggc aggaatggca gttgccacct ccctgtacgg gtgaacgccg cgttcggaga
     3121 atcaatggag agctcacgcc gtcagaaact aatagtttgc cgtgctcgcg ccgctatcgg
     3181 cagcgccgac gacatccgtg cagcgctcaa gcgagataac gaagctgtgc gggacagcga
     3241 caggacatca ggaacgaaag ccgtcagcgt ccgacctttt cgtcatcggc ttcagccatg
     3301 gcgctgtatt ccttgaggcg gttgtagagc gtcttgaggc tgatgccgag tgcagccgcg
     3361 gtctgctcct tctgcccgtc acagtgcttc agtgtcgcca ggatcacctt gcgctcgatg
     3421 tcggccagcg gcgtaccaag ttcgaccctc acactcgtcg cgtcggggcc gcttcggctc
     3481 ggcttcacgg cgccctcagg cagccagtct tctgtgatct cgctgtcagc cgccatgacg
     3541 tagcagcgat agacggcgtt gcgcagctcg cgcacgttac cgggccacgc ataggactcg
     3601 aagcgccgca gcacggcgct cgacagggtc ttcggctcgc cttccttctc gatgatttcc
     3661 tcgagaaagt gctgcgcgag aagaggtacg tcctcaagcc gttcgcgcaa tggcggcagg
     3721 tggatgggaa agacgttgag ccgatacagc agatcctccc gcagctttcc ttccgcgacc
     3781 gccagcagcg gatcgcggtt cgtcgcggca accaagcgga cgtcggcgtg cagggtctcg
     3841 agcgaaccga cccgcatgaa ggtacccgtc tcaagcacgc gcagcagctt gacctgcagt
     3901 tccgacggca tctcggtgat ttcatccagg aacagcgtgc cgccgtgtgc ccgctcgaag
     3961 taaccgaagt gctggcgcga ggccccggtg aacgcgccct tctcgtgtcc gaacagttcg
     4021 ctttcgatca ggttgggcga gatcgcaccg caattgacgg ccagggacgg acgctcgcgg
     4081 cggcgtgaaa gatcgtgcag cgtgcgggcc acgacttcct tgcccgtgcc gctctctccg
     4141 gtgatcagca cgctgacggc cgtcggcgca acgcgcccga tctgtgcata gatgcgcagc
     4201 atggcagccg aacggccccg caggtgcccg aatcgcgccg tgccttccgc ttcggacttc
     4261 agcgtctcga tctcgctgtc gatcgtcgcc ggactcgaca gtcgcgacag aatgcccttg
     4321 agctgcttga tattgaccgg cttgatcaga tagtccgccg cccccatgcg cagcgcatga
     4381 atggacgttt cgaggctggc gttgcccgtc accagcacga tttcggattc gactcgcact
     4441 ttctcatcgt cgagcaggct catgccgctg ccgtcaggca gttgcagatc gagcaggatc
     4501 aactgcgggg tctgcagcgc cagctgacgg cgcgcgccgg cgaggtcatg agccaccgcg
     4561 acggtcaggc cctcggtggc cagcagcgtc gccagcatct cggcggaatc gatgtcgtcg
     4621 tcgacgatca gggcatgcgt catggttcgt gcggcgcgtt cgcaccggag ttgtatcgga
     4681 ttgaattccc gcgaagcggg gacagggacc gtcgggagaa acgcaaattg cgtgccggcc
     4741 ggtctcgccc cgccgaggtt tcccatggac agtacaccgg ccacggcgcg gcgtagattg
     4801 aggcctgttc acccctgaat gcggaaacgg accaagaaga aggtgcagcg tgcccacagc
     4861 ataacggtcg cgcccagctt gccggactcg gggtcacgct tcgcacgcgc ccccgccgga
     4921 caaatagagg attcgcgata cgctgagtgg tcgtcagcgc aaccaaagtc aagtcgggca
     4981 agacccctct ctggggagcc cgctccaggt cgggttcaat ccttgtttgc gattccaaca
     5041 gccttgtccg gccccgtcac gatcgccctg atcgtcgtcc ttggcatctt tgcgatccca
     5101 tggctgctcg gacgcccgca cggacgctcc ctggtcgcca tcgcgacact gctgcttgcg
     5161 gcgttattca tggcgttcga ggccggcaac gaagcggcaa ccgtgacacg catcgtcctg
     5221 gcaggactgc tcggtttcgc gccggccgtg gccggcatca tcgtcgcgcg actgcagcgt
     5281 cgcgcgccgc cctgagggcc cgcagcaacg acttccgcgc ctgaattgcc gcgcgttggg
     5341 ccggcttttc cagcggatgc gcacttggaa cgcgcggcgc gctgcactac gctcgcgcta
     5401 ccacttcatc gggaaacccg atgcttccct ggcaggaact cgcactgaag attgcggtga
     5461 ccgcgctgct gttggcggtc gtgctgccac tggtcggctt cgcgttcacg cgcgacggct
     5521 cgtctggcct gcgggcgctg cgcactggcg cgatcatcct aggcgtgatt gcactggttg
     5581 cgggtgtcgc ggccctgctc ggcgaagtct ggcgctgaag gttccgccct cagactgacc
     5641 gcgttcgccc ctcgcgaaag ggcgtgcagc gaacgtcgac aacccgaaat acgcgttacc
     5701 cgctcacgcc ggcttcgacc tgctgcacct gtggtggcaa cggcgcgtcg agtgccgtgc
     5761 agatcatccg caatgagctc gcggccttcc agttggtctg accatcgaca aaggcaacgg
     5821 ccgtcgccgc cttgatcagc tgcggcttgg ccagcggcgc cagctggttc agccggtcga
     5881 gcgcgtcgga cacactgtcg agtcgcaggg acgacgcggc aacgaagggc agttccgcct
     5941 cgcgcagctg gactgcaccg gcgttgaagg cgtgttccgg acgctccggc aggcgcacca
     6001 tcgccaccag cgacagcacg acgccggcct cccgcgccac cgcggcaatg gcggaatagt
     6061 tcaccggcac cgggtgaccg gcatcgcgac cgagccgccg cttcaggatc gtgaacagga
     6121 gaaactcgcg cacggtcagt cggccgtcgg cggcgatcag cagatgcgcc gtgcgcaaca
     6181 gggcctcgcg cgcatcgtcc ggcaacttcc gcagtgccgg catcgcgaca tcgaccagcg
     6241 gctgacgcgc gccgggcggc aggcgctgga cctgctcgtg atagccgtcg gcaatctgcg
     6301 ccgctttgcc gccgtaggcc tcggcaatca gggcccgctg ctgcccacgc agttctcggt
     6361 ccttgtccac cagaaaggag agcacgagca gttgcgcgcg catcccgtcg gtggctgcgc
     6421 gccgcagtga ctccaccagg ccccgttgcg cctcgtccag cgcaaagtcg cgatgccccg
     6481 gcggtcgtac cgtgccgata tcggtcgcga tggacgccgc cgcg
//
LOCUS       DATIRT010000120         4627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1784573, whole genome shotgun sequence.
ACCESSION   DATIRT010000120 DATIRT010000000
VERSION     DATIRT010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4627
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1784573"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..772)
                     /locus_tag="VLN25_02930"
     CDS             complement(35..772)
                     /locus_tag="VLN25_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSQ09559.1"
                     /translation="MDTISQATIAALTMIFSFDPGLLSVVGRSLAVSTIACLLAFGFG
                     AIAGAWLGVSRFAGRGAALAVLNTLLALPSVVVGLLVYLLLSRSGPLGFLGWLFSFEA
                     MVLAQTILVAPIVTALTRQAIEDADRSHGEQLTSLGATPFMRGLLLVWDERYALLTVL
                     LAAFGRAISEVGAVMIVGGNIEGFTRVMTTAIALETSKGDLPLAIALGIVLLLVVLAL
                     NALIAALRNWRERLDGAATQAARVAVA"
     gene            891..2015
                     /locus_tag="VLN25_02935"
     CDS             891..2015
                     /locus_tag="VLN25_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HSQ09560.1"
                     /translation="MHKVELSYVLASGRAPEALIRNPLMDLLAAVRAQGSISGAARVL
                     GQSYRHVWGELKRWEQELGQRLIVWDRGQPARLTEFAEKLLWAERQVQARLAPQIAAL
                     HVDLERAFAMAFDPSASVLLLCASHDNALALLAEHCTPARLHLDIRFTGSANALHELN
                     AGHCAIAGFHLPPRTTSDSLIARSLKPLLRPERDQLIGFVRRVQGLALARGNPLNLHS
                     ITDVAARGARFVNRPSGTGTRMLLDALCEQHVVDRSALVGYENEEPSHAAVAQAVAAG
                     QADTGLCIEAAARSRDLDFVPLLWEEYFLVCDKATLEQPAVRALQAVLRSDSWSRRLS
                     TLAGYAPARSGEALSLRDVLPWWGERAQRESKQGFRKAGR"
     gene            complement(2111..2692)
                     /gene="mobA"
                     /locus_tag="VLN25_02940"
     CDS             complement(2111..2692)
                     /gene="mobA"
                     /locus_tag="VLN25_02940"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HSQ09561.1"
                     /translation="MRPVICGLVLAGGQGCRMGGVDKGLQPLQGRPLMQHVIDRLRPQ
                     VDSVLINANQNLERYAEFGCPVVPDRVGGFAGPLAGLDAGLHATVAPLIVTVPCDSPF
                     LPHDLVARLATARSAGDADVAVARTGSQPHPVFALVCARVRPHLADFLARGERKIDLW
                     YASLNVVEVAFDDEADAFANINTRAELDQHDRR"
     gene            complement(2751..3575)
                     /locus_tag="VLN25_02945"
     CDS             complement(2751..3575)
                     /locus_tag="VLN25_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003272366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HSQ09562.1"
                     /translation="MPLLLTNASRPLIFPVDAIDERGISVPTPIAGEHPLTLYVDKCE
                     VVTLMTLGQAPEALAIGYLRNQRLVSSLDELAAVQVDWETDAVAVTTRRGVKSLAKKL
                     AKRTVTTGCGQGTMFGDLMEEVDSIRLRDDVRLSEASLVAVLEAVRQHETIYNAAGAV
                     HGCALASNEGADSRILVFIEDVGRHNAVDAIAGRMWLDNIDGSDKIFYTTGRLTSEMV
                     IKTAQMGIPFLVSRSGLTEMAWRIASQVGLTMIGRAARKHYLLFTGRHRFDAARAS"
     gene            3736..4215
                     /locus_tag="VLN25_02950"
     CDS             3736..4215
                     /locus_tag="VLN25_02950"
                     /inference="COORDINATES: protein motif:HMM:NF023177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3305 domain-containing protein"
                     /protein_id="HSQ09563.1"
                     /translation="MANPLRRPAAVVTAKRLIDNPWTSEIWVPVAVIEREGARDDAPI
                     LLERSEAGEQWLHLGFEVELNRTEADGYYLNLTTDHPVAFVEWELTEVGAEPRWVTLS
                     YHEAARRMDGGAQVEAVPIPGEWLPWLAAFTQQHLKMPEKKRRPAPASFLGAKRDNR"
     gene            4277..>4627
                     /locus_tag="VLN25_02955"
     CDS             4277..>4627
                     /locus_tag="VLN25_02955"
                     /inference="COORDINATES: protein motif:HMM:NF023176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3306 domain-containing protein"
                     /protein_id="HSQ09564.1"
                     /translation="MSSEPESFLARWSRRKRAVDREAEPSAEEAAPLGADVSPAQAGD
                     EGVASGVPSPAPAPARESGEAAELPLPPIESLEGLRSDYTPFFLQPVGEDLRRAALKK
                     LFADPHFNKMDMLDV"
BASE COUNT          805 a         1487 c         1540 g          795 t
ORIGIN      
        1 ctcggaatgc cggcctgcgg gttgtcggac aggttcatgc gaccgcgacc cgtgcagcct
       61 gtgtggccgc tccgtcgagc cgttcgcgcc agttgcgcag tgcggcgatc agcgcgttca
      121 atgccagcac caccaggagc agcacgatgc cgagtgcaat agcgagcggc aagtcaccct
      181 tgctggtctc cagtgcgatc gccgtggtca tcacgcgggt gaagccttcg atattgccgc
      241 cgacgatcat caccgcgccc acttcggaaa tcgcgcgtcc gaacgcggcg agcagtaccg
      301 tcagcagcgc gtagcgttca tcccagacca gcaggagccc gcgcatgaac ggcgtggccc
      361 ccagcgacgt cagctgctcc ccatgcgacc ggtcggcgtc ctcgatcgcc tggcgcgtga
      421 gcgcggtgac gatcggagcc accaagatcg tctgcgccag caccatcgcc tcgaaggaga
      481 agagccaccc caggaagccc agcggtccgg agcgcgacag caacaggtag acgagcaggc
      541 cgacgacgac cgagggcagc gccagcaatg tgttgagcac agcgagcgct gcaccccgac
      601 ctgcaaagcg ggagacgcca agccaggccc cggcaatcgc accgaacccg aatgcgagca
      661 ggcatgcgat cgtgctgacc gccagcgaac gcccgacgac cgatagcagc cccgggtcga
      721 acgagaaaat catggtcagc gcggcaatgg ttgcctggga aatcgtatcc atagcggaat
      781 ggatcgtagg caagcgtgcg ggcaaccggc cccagcgagc cgggccacct taggcaatct
      841 gtttcatatg tagcgcgggc aagaggtgag ctttgcggca aactcgcatc gtgcacaagg
      901 tcgaactgtc gtatgtcctg gcgagcgggc gtgctcctga ggcactgatc cgcaatccat
      961 tgatggatct gctggccgca gtgcgcgctc agggttcgat ctcgggcgcc gcgcgagtgc
     1021 tgggccagtc gtatcgccat gtctggggtg agctcaagcg gtgggagcag gaactcggac
     1081 agcgcctgat cgtctgggac cggggtcagc cggcgcggct taccgaattc gccgagaaac
     1141 tactgtgggc cgagcgccag gtgcaggcgc ggttggcacc gcagatcgcg gcgctccacg
     1201 tcgatctcga gcgggccttc gcgatggcct ttgacccgag tgccagcgtc ctccttttgt
     1261 gcgccagcca tgacaacgct ctggcgttgc tggcggaaca ctgcactcct gcccgcctgc
     1321 acctcgacat ccggtttacc ggtagtgcga atgcactgca cgaactgaac gcgggacact
     1381 gtgctatcgc cggctttcat ttgccgccgc gcacgacctc ggacagcctg attgctcgaa
     1441 gccttaagcc gctgcttcgt cccgaacgcg accagctgat cggttttgtg cgacgcgtcc
     1501 agggcctggc gttggcacgc ggcaatccgc tcaacctgca ctcgatcacc gacgtggcgg
     1561 cgcgcggcgc tcgtttcgtc aaccggccgt ccggcaccgg cacgcggatg ctgctcgatg
     1621 cgctgtgcga gcagcatgtc gtggatcgat ccgcactggt cggctatgag aacgaggagc
     1681 cgtcgcatgc tgcggtggcc caggccgtgg ccgcaggtca ggcagacacc ggcctgtgca
     1741 tcgaggcggc agcgcgttcg cgcgacctcg acttcgtgcc gctgctgtgg gaggagtatt
     1801 tcctggtgtg cgacaaggcg acgttggagc aacccgccgt ccgcgccctg caggcggtgc
     1861 tgcgcagcga ttcctggtcg cggcggcttt cgacgctggc cggctacgcg cccgcgcgca
     1921 gcggcgaggc gctgtcattg cgcgatgtgc tgccttggtg gggagagcgt gcccagcgag
     1981 agtcaaaaca gggctttcgc aaggctggac gttgatcggg ctgtccgcga cgttttgcgc
     2041 gcgtgcgtcc gctcgtaatc agctcatggt cttccgacat cgggcgcgcg gatcgcgagc
     2101 gcccgcgaac tcatcgacgg tcgtgctgat ccagttcggc gcgcgtgttg atgttggcga
     2161 aggcgtcggc ttcatcgtcg aaggcgacct cgaccacgtt caggctcgcg taccagagat
     2221 cgatcttgcg ctcgccccgc gcgagaaaat cggccaagtg ggggcgcacg cgagcgcaga
     2281 ccagtgcgaa caccggatgc ggctgactgc cggtgcgcgc caccgcgacg tcggcgtcgc
     2341 cagcactgcg cgcagttgcc agccgggcga cgaggtcgtg cggcagaaac ggcgaatcgc
     2401 acggcacggt gacgatcagt ggagcaacgg tcgcgtgcag gcccgcgtcc aggccggcca
     2461 ggggccccgc gaagccgccg acccggtcgg gaacgacagg acaaccgaac tctgcatagc
     2521 gctcgaggtt ctggttggcg ttgatcagca cgctatcgac ctgcggacgc agtctgtcga
     2581 tcacatgctg catcagcggc cttccctgca gcggctgcag ccccttgtcc acgcccccca
     2641 tgcgacatcc ttgtccgccg gccagtacaa ggccgcagat cacgggcctc acgggacacg
     2701 cctctgcgct gcgcgctgcg gcatgagcgc tttggagcgg ccgtgcgcgc tcatgacgcg
     2761 cgcgccgcgt cgaagcggtg tcgacccgtg aagagcagat agtgtttgcg ggctgcgcgg
     2821 ccgatcatcg tcaggccgac ctggctcgca atgcgccagg ccatttcggt caggcccgag
     2881 cgtgacacca ggaagggaat ccccatctgc gccgtcttga tcaccatctc ggacgtcagc
     2941 cgccccgtgg tgtagaagat cttgtccgag ccgtcgatgt tgtcgagcca catgcgtcct
     3001 gcgatcgcat cgacggcgtt atgccgtccg acgtcctcga tgaagacgag gatccggctg
     3061 tcggcgccct cgttcgaggc aagtgcgcag ccgtgcacgg cgcctgcggc gttgtagatc
     3121 gtctcgtgct ggcgcacagc ttcaagtacg gcgaccaacg acgcttccga gaggcgcacg
     3181 tcatcgcgca ggcggatcga atcgacctct tccatcaggt cgccgaacat cgtgccctgt
     3241 ccgcagccgg tggtcactgt gcgcttggca agctttttcg ccagtgactt gactcctcgt
     3301 cgtgtggtga ccgccacggc gtcggtttcc cagtcaacct gcactgcggc gagttcatcg
     3361 agcgacgaca ccaggcgctg gttgcgcagg tagccgatcg cgagcgcctc gggcgcctgc
     3421 cccagtgtca tcagcgtgac gacttcgcac ttgtcgacat acagcgtgag cggatgctcg
     3481 ccggcgatcg gtgtcggcac cgagatcccg cgttcgtcga tggcatcgac agggaagatc
     3541 agtgggcggg acgcgttggt caacagcagt ggcatcgttg gtcgggttcc ggtcctgcgt
     3601 ggcgtctcga acgcatcgcg catcatcaag cgcgctccac aaagaccctt cgcgcgtatt
     3661 ccgtattcgg gtttacgctt gctgagcgat agcaggaata ttctccacag cctgaaggaa
     3721 gataacatcg aaggcatggc aaacccattg cgtaggccgg ctgcggtcgt gaccgccaag
     3781 cgccttatcg acaacccgtg gacgtccgag atctgggtcc cggttgccgt catcgagcgc
     3841 gagggcgcgc gcgatgacgc acctattctg ctcgaacgaa gtgaagcggg tgagcagtgg
     3901 ctgcatctcg gctttgaagt tgaactgaat cgcaccgagg ccgatggcta ctatctcaat
     3961 ctgacgaccg atcacccggt tgccttcgtg gaatgggaac tcacggaggt gggcgcagaa
     4021 ccgcgctggg tcacgctcag ctatcacgag gcggcgcgac gcatggacgg aggggcgcag
     4081 gtcgaggcgg taccgatccc aggcgagtgg ctgccgtggc tcgcggcatt cacacagcag
     4141 catctgaaga tgccggagaa aaagcgccgc ccggcgccgg catcgttcct cggcgccaag
     4201 cgcgacaacc gatgaatcct cgaggctttc tgcgtctccc tctcgacgtg aacggcgcgg
     4261 gtcgtgggct atctgaatga gctctgagcc ggaatcgttc cttgcccgct ggtcgcggcg
     4321 gaagcgcgcg gtcgatcgtg aggcggagcc gtcagctgaa gaggctgcgc cgctgggtgc
     4381 cgacgtttca ccggcgcagg ccggcgacga aggagttgca agtggcgtgc cttccccggc
     4441 tccggcgccc gcgcgcgaaa gcggcgaggc agccgagttg ccgctgccgc cgatcgagtc
     4501 gctcgagggt ttgcgttcgg actacacgcc gttcttcctg cagcccgtcg gtgaagacct
     4561 gcgccgggca gcactaaaga aacttttcgc cgacccgcat ttcaacaaga tggacatgct
     4621 ggatgtg
//
LOCUS       DATIRT010000121         3429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_408251, whole genome shotgun sequence.
ACCESSION   DATIRT010000121 DATIRT010000000
VERSION     DATIRT010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408251"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="VLN25_02960"
     CDS             complement(<1..840)
                     /locus_tag="VLN25_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09565.1"
                     /translation="MRAFGGLRAPVFLRCAAAALMALAVPTESLAQDALHGKRLYLDA
                     ARVRGSGVSCVDCHGGLPGGLFGIGRAANDPAAVERAVNAVPQMTPLRGRLGAQDYAD
                     LAAYLGNPTVPSPALRSLVSGPAATSSADRLDFGVVSRNAQSTASRWHIVNEGSVGLT
                     VTGAPQLRGHHPQDFVIIASDCSAGRTLMAGASCSVDLAFRPLDGVDTRQAAVAVAHD
                     WVGGEAAVALAGAVVAAPVSPAPSASAGGGGGGAALGLLCALLTAAAARRRCLAGPAT
                     DYRS"
     gene            complement(854..3091)
                     /locus_tag="VLN25_02965"
     CDS             complement(854..3091)
                     /locus_tag="VLN25_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HSQ09566.1"
                     /translation="MSHDDDVVTNTSSNPHINELIEQRLQDPARRKLLRGGFGVASLS
                     FIGASGLLSGCGGSDDVVATLVPPPAPAPTPTPAPPPPAPARPTALSFSAVSKSVADS
                     LVVPAGYTAAVLYRLGDPIAAAVPTYANNGTDAAATYDRRAGDHHDAIQYYGLGANGQ
                     YSATSNDRGLLVMNHEAITPAFLHPTGQTIVAGARTVADEVLREFYIHGVSVVEVNRA
                     AGGGAWSYKQDSTFNRRVHTLTETIISGPAGVTSYMITKYSTNGSRTRGTVNDCAHGY
                     TPWYTYLACEENWAGYFRRVTATDNPNRSAKELTSLARYGVAGNGRELWATVSPDTTD
                     NLYGRWNAEVRGPTAADDYRNGPNTYGWNVEIDPFNPTSTPKKRTAMGRFAHEGAWVA
                     KPVAGKPIVFYMGCDSRNEYIYKYVSTAVWDPADATLGLAAGDKYLDAGKLYVAKFNA
                     DGTGQWLELAFGIGNVTAGYAAYAFADQADVLVNTRLAADAQGATKMDRPEWGAVDPL
                     TGDVYMTLTNNNATLRTLAATDAANPRHYNDARTNGTAQRGNPNGHIIRWKEGSPEAL
                     TFTWDIFLFGARAGTDASNVNVSGLIAENDFSSPDGLWFSEATKVCWIQTDDGAYTDV
                     TNCMLLAALPGQVGDGGVKTITNTDQTGATKQVQTYVGAVLPSTKLRRFLVGPKDCEI
                     TGITETPDGRTLFVNIQHPGEETPAASIGDPTLFTSHWPEGGTARPRSATIAITRNDG
                     GVIGV"
BASE COUNT          570 a         1119 c         1138 g          602 t
ORIGIN      
        1 agatcggtag tcggtagcag ggccagccag gcatcggcgt cgagccgcgg cggcagtcaa
       61 cagcgcgcac aacagcccga gtgcagcgcc gcctccgccg ccgcctgcgc tagcggacgg
      121 cgcgggcgat accggcgcgg ccaccacggc gccagccagc gcgacggcag cctcaccgcc
      181 gacccagtcg tgtgccactg cgaccgctgc ctgacgagtg tcgacgccgt cgagcggccg
      241 aaaggccaga tcgacgctgc aggaggctcc ggccatcagc gtccggcccg ccgaacagtc
      301 gctcgcaatg atgacgaagt cctgcggatg atggccacgt agttgcggcg cgccggtcac
      361 ggtcaggccg accgagcctt cgttgacgat atgccagcgg ctcgccgtcg actgcgcatt
      421 gcgcgagacc acaccgaagt cgagccgatc ggcactcgac gtagccgcag gaccgctgac
      481 caggctgcgc aatgccgggc tcggcacggt cggattgccg aggtaggccg cgagatcggc
      541 gtagtcttgc gcacccagtc ggccacgcaa cggcgtcatc tgcggcaccg cattgacggc
      601 gcgttcgact gcggccggat cattggccgc gcgcccgatg ccgaacaggc cgcccggcag
      661 ccctccatgg cagtcgacgc agctcacgcc actgcctctc acgcgcgctg catcgagata
      721 gagacgtttg ccatgcagcg catcctgcgc gagcgattcg gtcggaacag cgagcgccat
      781 cagcgcagcc gccgcgcacc gaagaaaaac gggggcacgc aggcccccga aagctctcat
      841 cgccacgaat cctttacacg ccgatcacgc caccgtcatt gcgcgtgatc gcgatcgttg
      901 ccgatcgtgg ccgcgccgta ccgccttcgg gccagtggct cgtgaagagc gtcggatcgc
      961 cgatgctggc ggccggcgtt tcctcgcccg gatgctggat gttgacgaag agcgtccgcc
     1021 cgtcgggcgt ctcggtgatg ccggtgatct cgcaatcctt cggacccaca aggaagcgcc
     1081 gcagcttggt cgacggcagg accgcgccga cgtacgtctg cacctgtttg gtggcgccgg
     1141 tctgatcggt gttcgtgatc gtcttcacac caccgtcgcc gacctggcca ggcagtgccg
     1201 cgagcagcat gcagttggtc acgtcggtat acgcgccgtc gtcggtctgg atccagcaga
     1261 ctttggtcgc ctcgctgaac cacaggccgt ccggactgga gaagtcgttc tcggcgatca
     1321 atcccgaaac gttgacgttg ctcgcatcgg tgcccgcgcg ggccccgaac aggaagatgt
     1381 cccacgtgaa cgtcagcgcc tccggtgaac cttccttcca gcggatgatg tggccattcg
     1441 gatttccgcg ctgggcggtg ccgttggtgc gcgcgtcgtt gtagtggcgc ggattggccg
     1501 catcggtggc cgccagcgtg cgcagcgtcg cgttgttgtt ggtgagcgtc atgtagacgt
     1561 cgccggtcag ggggtcaacc gcgccccact ccggccgatc catcttcgtc gcgccctgtg
     1621 catcggcggc gagacgcgta ttgaccagga cgtcggcctg atcggcgaac gcataggctg
     1681 catagcccgc ggttacgttg ccgatgccaa acgcgagctc cagccactgg ccggtgccat
     1741 ccgcattgaa tttcgcgacg tagagctttc ctgcatcgag atacttgtcg ccagcggcca
     1801 ggcccagcgt cgcatctgcc ggatcccaca ccgccgtcga cacgtacttg tagatgtatt
     1861 cgttgcggga gtcgcacccc atgtagaaga cgatcggttt gcctgccacc ggcttggcta
     1921 cccaggcccc ctcgtgggcg aagcggccca tggcggtgcg cttcttcggt gtcgaggtcg
     1981 ggttgaacgg gtcgatctcg acgttccagc cataggtgtt cgggccgttg cgatagtcgt
     2041 cagccgcggt cggccccctg acctcggcgt tccagcgccc gtagaggttg tcggtcgtgt
     2101 cgggagagac agtggcccag agttcgcggc cattgccggc gacgccatag cgagcgaggg
     2161 aagtcagttc cttggcgctg cggttcgggt tgtcggtggc ggtgacacga cggaagtatc
     2221 cggcccagtt ctcctcgcag gcaaggtacg tataccaagg cgtgtagccg tgcgcgcaat
     2281 cgttgacggt tccgcgggtg cggctcccgt tggttgagta cttggtgatc atgtatgacg
     2341 tcacgcccgc cggaccggag atgatcgtct cggtcagcgt gtggacacgc cggttgaacg
     2401 tggagtcctg cttgtagctc cacgcgccgc caccggccgc gcgattgacc tcgacgacgc
     2461 tcacgccgtg gatgtagaac tcgcgcagca cctcgtcagc caccgtgcgc gcgccggcga
     2521 cgatcgtctg gccggtcgga tgcaagaacg ccggcgtgat cgcctcgtgg ttcatcacca
     2581 gcagaccgcg atcgttcgac gttgcgctgt actggccgtt ggcgccgagt ccgtagtact
     2641 ggatcgcatc gtgatgatca cccgctctgc ggtcataggt cgcggcggca tcggtgccgt
     2701 tgttcgcata ggtcggaaca gccgccgcga tcggatcgcc gagccgatac agcacggctg
     2761 cggtgtatcc cgccggaacg accagggaat cggccaccga cttggatact gcagaaaaac
     2821 tcaacgctgt cggccgcgcg ggcgcggggg gtggaggcgc cggtgtcggc gttggagcag
     2881 gtgccggagg cggcacgagc gtcgccacga catcgtcgct gccaccgcag cctgacagca
     2941 ggcccgatgc gccgatgaac gagagcgacg ccacgccgaa gccgccgcgc agcaacttgc
     3001 gccgcgcggg gtcctgcaga cgctgctcga tcagctcatt gatgtgggga ttggacgagg
     3061 tattcgtgac gacgtcatcg tcatgcgaca tgggcaatgc ctccggttgc ggtcgagcgg
     3121 gttgcggaaa tgcgcaagtg tcgcgatgtc cgatgaccaa cgcgtttcga aacgatgaag
     3181 cccccgtgac gtagggtctt cgctgcaact ttcgaatgca cttgaagaag cggctgcaag
     3241 ttcccgtgtc atatcggagt caccggacct ccctaccctg cgttcctcag ggtgcgatgg
     3301 cttcagctca aagtcgcgtt gtggagctca acgagcacga cgacgctccg aacagcgtgt
     3361 gcgatcgatc cacaacatgc gttgccgctc ttcaacgcgg ttaccgaacc gccggacgag
     3421 ccggcctcg
//
LOCUS       DATIRT010000122         2854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_899792, whole genome shotgun sequence.
ACCESSION   DATIRT010000122 DATIRT010000000
VERSION     DATIRT010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899792"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="VLN25_02970"
     CDS             complement(<1..941)
                     /locus_tag="VLN25_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSQ09567.1"
                     /translation="MALLHAPLAAKPVEQVRVGFSTSISGPFAALGAEARDGFNLGVK
                     QLGGKLGGIPVEVFATDEAGNPDTARQHVDRYIKRDRIHFYTGPIGSNVALAVGPALF
                     AAEVFYLSNNAGPSQFAGAQCNTYFFGTAWQNDAYHEAAGKFAGERGFKKVAVLAPNY
                     PAGQDAITGFKRGFARQVASETFTKLGQLDYAAEIAKLRAEAPDALYIFLPGGMGINF
                     IKQFVGAGLSKITQIVGPGFSADEDVIRAVGDPMLGMFNTAHWNHDFLNPASQAFVQA
                     YQKEYGRLPSIYEAQAYDTVLMLDAAVRDVKGELDNRA"
     gene            1230..>2854
                     /locus_tag="VLN25_02975"
     CDS             1230..>2854
                     /locus_tag="VLN25_02975"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSQ09568.1"
                     /translation="MSGPLTRFGLVVETQEQRKEKILAGVMASVYRQVPQSVLVSIVG
                     AFALLVVFWNSMSQNLLMGWFVLILLESLIRVRVAYRFRHASAVVDQVQRWAISWVVL
                     AVTAGLLWGVAGFLFFSNDQPLHQVVLVAVVLSVAFGSLTLYACYRPSFYSFMLLALL
                     PLIARMVSEQDPTYYTASVVMAAVFFFTVFYGRTFGDAVFESVKNNYENEVLVEQLLN
                     EKRLAEEARRDAENATRSKTQFFAAASHDLRQPLQAIGIYVSLLKKRAQGPLEPLVNN
                     LSTAVESLSKLVEELLEISRLDSGAIQPRVDQLLLDDLFTLLEQEFTPLSASKGLSLR
                     VRRHGFAIDSDPLLLQRVIRNLLANAIRYTQRGGVLLAARPRNGLISIEIWDTGPGIK
                     QGELDRIFEEFYRGESSKAENSGAGFGLGLSIVKRICGLLGHPLIVTTRPGTGTVFRV
                     EVPLSIAPPKAKRTAPETIDVAIRALNGFTFVVLEDNAEILNSLTRLLRSWGAEVVPS
                     TGYNAQLIKRISLHERIDAIIADHNLGPHSISGV"
BASE COUNT          496 a          914 c          918 g          526 t
ORIGIN      
        1 gcccggttgt cgagttcgcc cttcacgtcg cgcacggcgg catccaacat cagcaccgtg
       61 tcgtaggcct gtgcctcgta gatcgacggc aggcgaccgt actccttctg gtaggcctgc
      121 acgaacgcct ggctcgccgg attcagaaag tcgtggttcc agtgcgcggt gttgaacatg
      181 ccgagcatcg gatcgccgac cgcacggatc acgtcctcat ccgccgagaa cccgggaccg
      241 acgatctgtg tgatcttcga cagcccggca ccgacgaact gcttgatgaa attgatgccc
      301 atacctccgg gcaggaagat gtagagcgcg tccggtgcct ccgcgcgcag cttcgcgatc
      361 tcagccgcgt agtcgagctg gccgagcttg gtgaatgtct cggacgcaac ctgcctggcg
      421 aagccgcgct tgaacccggt gatggcgtcc tgcccggccg gatagttcgg cgcgagcacc
      481 gccaccttct taaagccgcg ctcgccggcg aacttgcccg cggcttcatg gtaggcatcg
      541 ttctgccagg cagtgccgaa gaagtaggtg ttgcactggg ctcccgcgaa ttgcgacggc
      601 ccggcgttgt tgctgaggta gaacacctcg gcggcaaaca gcgccggtcc gacggcaagc
      661 gccacgttcg agccaatggg cccggtgtag aagtgaatcc ggtcgcgctt gatgtaccgg
      721 tccacatgct gacgcgcggt gtccgggttg cctgcctcat cggttgcaaa cacctcgacc
      781 ggaatgcccc cgagcttgcc gccgagttgc ttgacgccga ggttgaagcc gtcgcgtgcc
      841 tccgcaccga gcgccgcaaa gggtcccgaa atcgaggtcg agaagccgac acgcacctgt
      901 tcgaccggtt ttgccgccaa tggtgcatga aggagcgcca gcgagcaggc ggcggcgaga
      961 actgcggatt gggacttctt caagacgagg ctccggagcg ggtggggtag ttgcaggacg
     1021 cgcaagtatt cggaaaccga aggggcgcgt caaatggcgc gcgtcaaaca gagacggccc
     1081 ttcgcagccg atgctgcagc ggtggatgga taacaatccg aaaaacaggc aaacggctgc
     1141 ccaaaccgac gtgatgctgt aggcgtcggt cgctgtgcgc aaatgcgctt cccgcctatc
     1201 atcgcgcctg tcgcccaaac cagtcgcgca tgtccggacc tctcacccga ttcggcctcg
     1261 tcgtcgagac gcaggaacag cgcaaggaga agatccttgc cggcgtgatg gcatcggtct
     1321 atcggcaggt gccgcagtcg gtgctcgtca gcatcgtcgg tgccttcgcg ctgctggtcg
     1381 tgttctggaa ctcgatgagc cagaacctgc tgatgggctg gttcgtgctg atcctgctcg
     1441 aatcgctgat ccgcgtgcgg gtggcctatc ggttccgcca cgcgtccgcc gtggtcgacc
     1501 aggtccagcg ctgggcgatc agctgggtcg tgctcgccgt caccgccggc ctgctgtggg
     1561 gcgtcgcggg cttcctcttc ttctcgaacg accagccact gcaccaggtg gtactggtcg
     1621 cggtcgtgct cagcgtggcc ttcggctcgc tgacgctgta cgcctgttat cggccttcgt
     1681 tctactcgtt catgctgcta gcgctgctgc cgctgatcgc ccgcatggtg tcggagcagg
     1741 acccgaccta ctacacggcc tcggtggtga tggccgctgt gttcttcttc acggtgttct
     1801 acggtcgtac cttcggcgat gcggtgttcg agtcggtcaa gaacaattac gagaatgagg
     1861 tcctggtcga gcagctgctg aacgagaagc ggctggccga ggaggcacga cgcgacgccg
     1921 agaacgcgac gcgctcgaaa acccagttct tcgcggcggc aagtcacgac ctgcgccagc
     1981 cactgcaggc gatcggcatt tatgtctccc tgctgaagaa gcgtgctcag ggcccgctgg
     2041 aaccgctcgt gaacaaccta tcgacggcgg tcgagtcgct gtcgaagctg gtggaagaac
     2101 tgctggagat ctcgaggctg gactcaggcg cgatccagcc gcgcgtcgac cagctgctgc
     2161 tcgacgacct gttcactctg ctggagcagg agttcacgcc gctgtcggcc tccaaggggc
     2221 tgtcactgcg cgtgcgtcgc catggctttg ccatcgacag cgatccgctg ctgctgcaac
     2281 gggtcatccg caaccttctg gccaacgcga tccgctacac gcagcggggc ggggtgctgc
     2341 tcgcggcgcg cccgcgcaat ggcctcatca gcatcgagat ctgggacacg ggtccgggca
     2401 tcaagcaggg cgagctcgat cgcatcttcg aggagttcta tcggggcgaa agctccaagg
     2461 ccgagaacag cggcgccggg tttggcctgg gcctgtcgat cgtcaagcgc atctgcgggc
     2521 tgctcggaca tccgctgatc gttacgaccc gtccggggac cgggaccgtc ttccgcgtcg
     2581 aagtgccgct gtcgatcgca ccgccgaagg ccaaacgcac ggcacccgag acgatcgatg
     2641 tcgcgattcg cgccctcaac ggcttcacct tcgtcgtgct cgaagacaat gccgagatcc
     2701 tgaacagcct gacgcgactg ctgcgctcgt ggggcgccga ggtcgtgccc tcgaccggct
     2761 acaacgcgca gctgatcaag cgcatcagcc tgcacgagcg catcgacgcg atcatcgccg
     2821 accacaacct cggaccgcat tcgatcagcg gcgt
//
LOCUS       DATIRT010000123         2768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1882849, whole genome shotgun sequence.
ACCESSION   DATIRT010000123 DATIRT010000000
VERSION     DATIRT010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1882849"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VLN25_02980"
     CDS             complement(<1..418)
                     /locus_tag="VLN25_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09569.1"
                     /translation="MLELGSFEDARRKVLANSQRLLLGVKRAVAEPIDSVDAGLAMLA
                     SLRRQAHDGLIHIQRDQMIICAAEWLVTHGVASSATCWNWNPRSSTEPAAPDLIGRDG
                     DSAVICAEVAASEEPVGVIDAQMRRALAKLASMDGRR"
     gene            complement(675..1289)
                     /gene="msrA"
                     /locus_tag="VLN25_02985"
     CDS             complement(675..1289)
                     /gene="msrA"
                     /locus_tag="VLN25_02985"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HSQ09570.1"
                     /translation="MRTLRWTWAVAAVWVAAVGAQPKADVPPRTEIAIFAGGCFWCVE
                     ADFDKVDGVLETTSGYIGGRIPNPTYSAVSAGATGHTEAVKIVFDPARISYAQLLEKF
                     WPTIDPTVKDQQFCDVGSQYRSGIFPLDQKQLQEAEASKAARQKSKPFKAPIVTEITL
                     ATTFYPAEEYHQDYYLKNPIRYRYYRTGCGRDARLKDLWGRPPQ"
     gene            complement(1289..1798)
                     /gene="msrB"
                     /locus_tag="VLN25_02990"
     CDS             complement(1289..1798)
                     /gene="msrB"
                     /locus_tag="VLN25_02990"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HSQ09571.1"
                     /translation="MMSRRHLLSRLFLGGLFARAVTAVAAPAGPAPSSPAVRTDKLVL
                     TDAQWRQRLTSDQYRVLRRDGTEPAFSSPLNAEKRRGTFKCVACDLPLFTSAMKYDSG
                     TGWPSFFETLPGAFEVSTDYTLIFPRTEYHCARCGGHHGHVFNEGPRPTGKRYCNNGV
                     CLKFEAEAA"
     gene            complement(1948..>2768)
                     /locus_tag="VLN25_02995"
     CDS             complement(1948..>2768)
                     /locus_tag="VLN25_02995"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HSQ09572.1"
                     /translation="LLGQRASLSYLGILAEIARNSATVGEALCTFSVNQHLYSGGGAV
                     YLFQDNDEITFGYAIYHAGLRGCEQIHDLAMAQMLSGVRELCGGTFQPRIIELSRHAP
                     ADAAPYRRAFPAPVVFDSVNSAMRFGADVMRQPIAGADPARLRELNELLEAAGRHNLL
                     NRLRRTLRIQLLRGDTSGDHAAQMLDMHRRTLNRRLMRRGTTFQKVLDQVRFDAARHL
                     LYLTRIPVPRIASSLGYSNASSFTRAFRRWSGSTPARYRAVPVSGVGPSVHGRS"
BASE COUNT          494 a          893 c          891 g          490 t
ORIGIN      
        1 acctgcgccc gtccatggac gccagtttcg ccagcgcccg cctcatctgc gcgtcgatca
       61 cgccgaccgg ttcctcggat gcagccacct cggcacatat cactgcggaa tcgccgtcgc
      121 gtccgatcag atcgggcgcc gcgggctcgg ttgagctacg tggattccaa ttccagcagg
      181 tcgcagaact ggccacacca tgagtcacca gccactcggc ggcgcagatg atcatctgat
      241 ctcgctggat atggatgagg ccgtcatgcg cctgccgacg cagactcgca agcatggcca
      301 ggccggcgtc caccgagtcg atcggctccg ctacggcacg cttgacgcca agcagcagcc
      361 gctgtgaatt ggcaagcacc ttacgccggg catcttcaaa agatccaagt tcaagcattg
      421 caggtcaggg acgcggcacg gttttttgat ggacaggcca cggggcatca ggcatcgacc
      481 ccgtgatcgg ctggtcggtc gcaaggtaat cggcttttcg cggtctgcga agtcctgcgc
      541 ctcgcctgac ttgggtcggt tcacatcggc catgtggacg gtgcgactgc gacccaccgc
      601 gtggccgtgg gatgcaatcc catttgcctc gctactgccg cgcgccgaat gcctgggccc
      661 acgttgatgc gctcttactg cggcggtcgg ccccacaggt ccttgaggcg cgcgtcacgg
      721 ccgcagccgg tgcggtagta ccgatagcgg atcggattct tcaggtagta gtcctggtgg
      781 tattcctcgg ccggatagaa cgttgtcgcc aaagtgatct ccgtgacgat cggcgccttg
      841 aatggcttgc tcttctgtcg cgcggccttc gatgcttcgg cctcctgcag ctgcttctga
      901 tcgagcggga atatgcctga acggtactgc gatccgacat cgcagaactg ctgatccttt
      961 accgtcggat cgatcgtggg ccagaacttc tcgagcagtt gcgcgtagct gatgcgggcc
     1021 ggatcgaaga cgatcttcac ggcctcggtg tggccggtag cgccggccga taccgcgcta
     1081 taggtcgggt tcggaatgcg accgccgatg taccccgacg tggtctcgag caccccgtcg
     1141 accttgtcga agtcagcctc gacacaccag aagcagccgc cggcgaaaat ggcgatctcc
     1201 gtccgcggcg gcacgtccgc cttcggttgt gcgcccaccg cggcaaccca cacggccgcc
     1261 actgcccagg tccagcgcag ggtccgcatc aagcggcctc agcttcgaac ttcaggcaca
     1321 cgccgttgtt gcagtagcgc ttgccggtcg ggcgtggccc ttcattgaac acatgaccgt
     1381 ggtgaccgcc gcagcgtgcg cagtggtact ccgtgcgcgg aaaaatcagc gtgtagtcgg
     1441 tgctgacctc gaacgccccg ggcaaagtct cgaaaaaact cggccagccg gtgccgctgt
     1501 cgtacttcat cgcactcgtg aacaacggca ggtcgcaggc cacgcacttg aacgtcccgc
     1561 gccgcttctc ggcattcagc gggctgctga acgcaggctc ggtgccgtcc cggcgcagga
     1621 cccggtactg atccgacgtc agccgctggc gccactgcgc gtcggtcagg acgagcttgt
     1681 cagtgcgtac agcggggctg gatggcgcgg gtccggccgg cgcggcgacg gcggtaaccg
     1741 ctcgcgcaaa caatccaccc aggaacaagc gggacagcag atggcgacgt gacatcatgg
     1801 actttcctcg gtcatcaatc tgaaggaccc cgatggcgcg ggacagttcc cgcaatcggc
     1861 cctaccgcga ttggtcgccg ggcgggtgaa gtccttacaa agtcacggcg gcgggctgcg
     1921 gccatctatc cgggatcagg gggctttcta ggatcggcca tgcaccgacg gccctacgcc
     1981 cgagacgggc accgcacgat agcgggccgg cgtgctgccc gaccagcggc gaaacgcccg
     2041 cgtgaacgac gacgcattcg aatagccaag cgacgaggca atgcgtggaa cggggatccg
     2101 ggtgaggtac agcaggtgtc gggccgcatc gaagcggacc tggtccagca ctttctgaaa
     2161 cgtggtaccg cggcgcatga ggcgtcggtt caaggtgcgc cggtgcatat cgagcatctg
     2221 cgcggcatga tcgccgctgg tgtcgccgcg caaaagctgg atccgcagcg tccgtcgcag
     2281 ccggttcagc aggttgtgcc gcccggccgc ttcgagtaat tcgttgagtt cccgcaaccg
     2341 cgcagggtcg gcgccggcga tcggctgccg catgacgtcc gcgccgaagc gcatcgcgct
     2401 gttcacggaa tcgaacacga caggcgccgg aaaagcgcgc cggtacgggg ccgcgtccgc
     2461 aggcgcgtgg cgggataatt cgatgatgcg tggctggaac gttcccccgc acagttctcg
     2521 cactccgctg agcatctgtg ccatcgccag gtcgtggatc tgctcgcagc cgcgcaaccc
     2581 cgcgtgatag atcgcgtagc cgaacgtgat ttcgtcatta tcctggaaca ggtagacggc
     2641 gccgccgccg ctataaaggt gctgattaac ggaaaatgtg catagcgctt cgcctaccgt
     2701 cgccgaattg cgggcgatct cggccaggat gccgagatac gacagggaag cacgctggcc
     2761 gagcagca
//
LOCUS       DATIRT010000124         2594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_408549, whole genome shotgun sequence.
ACCESSION   DATIRT010000124 DATIRT010000000
VERSION     DATIRT010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408549"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..623
                     /locus_tag="VLN25_03000"
     CDS             63..623
                     /locus_tag="VLN25_03000"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HSQ09573.1"
                     /translation="MRYCPTCGSPITLRVPGGDTRVRACCDQCGAIHYVNPRPVVGTI
                     PIWGDEILLCRRAIEPRYGKWTLPAGFMEVGETTADGALRETMEEAGARVALGPLFSV
                     IDVPHVEQVHIFFQARLLDLGFAPGEESLEVQLFTEHTVPWPELAFRTVASTLRHYFA
                     DRARGAFGVHTEAIAPGVPQPHVPAA"
     gene            620..1357
                     /gene="aat"
                     /locus_tag="VLN25_03005"
     CDS             620..1357
                     /gene="aat"
                     /locus_tag="VLN25_03005"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HSQ09574.1"
                     /translation="MIPWLAGGQPFPDVDTALRSPNGLLAASTDLTVERLIEAYSKGL
                     FPWSAEGEPVLWWSPDPRMVLYCSEFRVSRSLQKVLRRTARSHTVEIWIDRAFEDVMR
                     ACASPRSDDAGTWITPDVIRAYGALHARGLAHSVETWIDGRLVGGLYGVSMGRMFFGE
                     SMFARAPDASKLALAALVRILLNEHVPVIDCQQNTRHLASLGGREIARHQFCSHVHKA
                     VQMTPIDWGRYRHRLNEQLAAFERPAD"
     gene            1359..2123
                     /locus_tag="VLN25_03010"
     CDS             1359..2123
                     /locus_tag="VLN25_03010"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HSQ09575.1"
                     /translation="MANLKELPFSALQFYATAPYPCSYLEDRQARSQVATPAHLINAD
                     VYSELVKMGFRRSGIFTYRPYCDGCRACVPVRVPVAEYRPNRTQRRAGQRHVDLVSHV
                     GTLAFDAEHYDLYLRYQASRHAGGGMDNDSREQYSQFLLQSKVNTRLVEFRDPVTDGG
                     AGPGILRMVSIIDLLNDGLSSVYTFFDPEVLTASFGTFNILWQIEQCRALKLPYLYLG
                     YWIAESSKMAYKARFRPVEILRGGRWERLREPALKA"
     gene            2165..>2594
                     /locus_tag="VLN25_03015"
     CDS             2165..>2594
                     /locus_tag="VLN25_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HSQ09576.1"
                     /translation="MLYPLARSVLFALDPEVAHELTLSKLEHAAAFGLTRLIARRVHD
                     LPVQAMGLTFRNPVGLAAGMDKNAAHIDALGSFGFGHIEAGTVTPRLQPGNPKPRLFR
                     LIPAQGLINRMGFNNDGVEHFVANVRAQQRFRAAGGVVGLN"
BASE COUNT          445 a          866 c          814 g          469 t
ORIGIN      
        1 cggcggggca gggagtcgga tacgagcctg cgatagactg atttccgaag ggagtcctct
       61 cgatgcgtta ctgcccgacc tgcgggagtc cgatcaccct gcgcgtgccg ggcggcgata
      121 cgcgggtacg tgcctgctgt gaccagtgcg gagcgatcca ttacgtgaac ccgcgcccgg
      181 tcgtgggcac gatcccgatc tggggcgacg agatcctgct gtgccgccgt gcaatcgagc
      241 cacgctacgg caagtggacg ctccctgccg gattcatgga agtcggcgaa accactgcgg
      301 acggggcgct gcgtgaaacc atggaggaag ccggcgcccg cgttgcgctg ggccccctgt
      361 tttccgtgat agacgtgccg cacgtcgaac aggtccatat cttcttccag gccaggctgc
      421 tcgatctcgg ttttgcgccc ggtgaagaaa gcctcgaagt gcagctcttc accgagcaca
      481 cggtgccatg gccggagctg gccttccgca cggtcgccag caccctcaga cactactttg
      541 cagaccgggc ccgcggtgca ttcggcgtac atacggaagc catcgcgccc ggagtgccgc
      601 agccccacgt tcctgccgca tgatcccgtg gctcgccggc gggcagccgt ttcctgacgt
      661 cgatactgcg cttcgctcgc ccaacgggct gctcgcggcc agcacggacc tcaccgtcga
      721 acggctgatc gaagcctatt ccaagggcct cttcccgtgg tccgcggaag gcgaaccggt
      781 gctgtggtgg tcgcctgatc cgcgcatggt gctgtactgc tcggagttcc gcgtttcgcg
      841 ctcgctgcag aaggtgctgc gccgaaccgc gcgcagccac accgtcgaga tctggatcga
      901 ccgtgccttc gaggacgtga tgcgcgcgtg cgcatcccct cgatcagacg acgccggcac
      961 ctggatcaca cccgacgtca tccgggccta tggcgcgctg catgcgcgcg ggctggcgca
     1021 ttcggtcgaa acatggattg acggacgcct cgtgggcgga ctctacggcg tcagcatggg
     1081 ccggatgttc ttcggcgaat cgatgttcgc ccgcgcaccc gacgcctcga aacttgcact
     1141 cgccgccctg gtgcgaatcc tgctgaacga acacgtgccg gtgatagact gccagcagaa
     1201 cacgaggcac ctggcctcgc ttgggggacg tgagatcgca cgacaccagt tctgttcgca
     1261 tgtacacaaa gcggtccaga tgacgcccat cgactggggg cggtaccggc accggctgaa
     1321 cgagcaactg gctgcgttcg aacgaccggc ggactgacgt ggcaaacctc aaggaactcc
     1381 cgttctcggc gttgcagttc tacgccaccg cgccctaccc ctgcagctac cttgaagacc
     1441 gacaggcgcg ttcgcaggtg gccacaccgg cgcacctgat caacgccgat gtctatagcg
     1501 aactggtcaa gatgggattc cgccgcagcg gaatcttcac ctaccgcccg tattgcgacg
     1561 gatgccgcgc ctgtgtgccg gtccgagtac cggtcgccga gtaccgaccc aaccggaccc
     1621 agcgtcgcgc gggccagcgg cacgtcgatc tcgtttcaca cgtcggcacg ctggccttcg
     1681 atgccgagca ttacgacctg tacctccgct accaggccag ccgtcatgcc ggcggcggca
     1741 tggacaacga cagtcgcgag cagtattcgc agttcctatt gcagagcaag gtcaacacac
     1801 ggctggtcga gttccgcgat cccgtgactg acggcggtgc cggtccgggc atcctgcgca
     1861 tggtgtcgat catcgacttg ctcaacgacg ggctctcgtc cgtgtacacg ttcttcgatc
     1921 cggaggtact gaccgcaagc tttggcacgt tcaacatctt gtggcagatc gagcagtgtc
     1981 gcgcgctgaa gctgccgtac ctttatctcg ggtactggat cgccgagagc tcgaaaatgg
     2041 cctacaaggc gcgcttccgt cctgtcgaga tcctgcgcgg cggccgctgg gaacggctgc
     2101 gcgaacccgc gctcaaggct tagccccgcg cacgtgcggc tatgcgggcc gctaagattg
     2161 cggcgtgctc tacccacttg cacgttccgt tctgttcgcg ctcgatcccg aggtcgcgca
     2221 cgaactgacg ttgtccaagc tcgaacatgc ggcagcgttc gggctgacgc gcctcatcgc
     2281 acgccgggtt catgacctgc ctgtacaggc catgggactg acgttccgca atcccgtcgg
     2341 cctggccgcc ggcatggaca agaatgcggc gcacatcgac gcactcggca gtttcgggtt
     2401 cggtcatatc gaagccggca ccgtgacgcc gcgactccag cctggaaatc cgaagcctcg
     2461 cctgttccgc ctgattccgg cgcagggcct gatcaaccgc atgggcttca acaacgacgg
     2521 cgtcgagcac ttcgtggcca acgtgcgagc gcagcagcgc tttcgtgcag cgggcggcgt
     2581 ggtgggcctc aata
//
LOCUS       DATIRT010000125         6017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_211994, whole genome shotgun sequence.
ACCESSION   DATIRT010000125 DATIRT010000000
VERSION     DATIRT010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6017
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211994"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="VLN25_03020"
     CDS             <1..1197
                     /locus_tag="VLN25_03020"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HSQ09577.1"
                     /translation="VLKAALMDAAFFASSAHPARKLIDRIAGTSAGWEPYGDDNQKYL
                     KEVERVIGEILKKFETDLSVFEKVYTEFDRFVSEIDPGDSDPVSRAKKALEAAEKHEI
                     LTINTTIQVRRAFERVDLEPYIKEFLLGPWVKVLVAATVRSETQPGFAKSFRDVIHEL
                     VWSVQPKASTEDRNKLVKMIPTMVRVLRDGLALVNQSERDRDQFFTSLMESHAMAVKP
                     VDQATYIKSSLLTSELRAKIDGMQLSGIFPVTTVPGGIRVSTGLIMRAAQEHHADIQM
                     PDPLTDIARLDPVEDARVQEEIGRWQRGNWFKMWNGMEFVRVKLRWMSPLRTLFLFAG
                     EHDQKAHVLSSDMLKSYLARKYIEPLESVPLTKRAVDAVVADFESAPDRATNLAKRYK
                     ASQPAA"
     gene            1343..3043
                     /locus_tag="VLN25_03025"
     CDS             1343..3043
                     /locus_tag="VLN25_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HSQ09578.1"
                     /translation="MLATSDAERFKISGGRDRAGHPLVNLAEMNYRTPNAERKLLDLE
                     ALTQWLAQKSSLPYFHIDPLRVDFTRVVDVMSSSYATTYSILPVAMGGDEVTIATCEP
                     FQTGWEREIEQITKKKVKRVVSNPLDVARYTTEFYKLAQQVRGAAKSGSAPLVSSFEQ
                     LVELGGKVEANDAHVIHIVDWLLQYAFDQRASDIHLEPRREFGVARFRIDGVLHQVYQ
                     VPGGVMAAMTSRIKLLGRMDVVEKRRPQDGRIKTKVATGPNAGREVEIRLSTLPTAFG
                     EKLVMRIFDPEVVVRSYAELGFTPDETKVWEDLIHRPSGIVLVTGPTGSGKTTTLYST
                     LKQLATDEVNVSTVEDPIEMVDPALNQMQVQHGIDLGFADGLRALMRQDPDIIMVGEI
                     RDKETADMAVQSALTGHLVFSTLHTNDAAASIARLLELGIPNYLVNATLIGILAQRLV
                     RTLCQHCKAPGDPIEPMLWKDLVAPWRSNVPEKIYHPVGCLECRRTGYMGRTGLIEML
                     VMSDAMRKLIGEGADLVQLRGQAARDGLRSLRVSGAEKIAKGMTTLDEVLKAAPPFNN
                     "
     gene            complement(3050..4246)
                     /locus_tag="VLN25_03030"
     CDS             complement(3050..4246)
                     /locus_tag="VLN25_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09579.1"
                     /translation="MLIDFFFHLRAAKLKVSINELLTLLEALKQGVIGPQSPEGGLSL
                     LDEFYHLSRTTLVKDETQFDKFDRAFGEYFKGVTTSVDFTKEIPDEWFRKVLERAFSP
                     EEMAKLEKLGWDKLMEEFRKRLEEQDGAHHGGSRWIGSGGTSPFGHGGANPEGIRVGG
                     PSKGGKSAVKVWEMRGFRDYDDSVEIGTRNIKVALRRLRRFAREGADLELDMHDTIES
                     TAKNAGWLDLKLVPERHNTVKVLMLLDVGGTMDEHIKRTEELFSAAKGEFKHLEFYYF
                     HNCVYEYLWRNNRRRYTERFATWDVLRKYPHDWKLVFVGDATMSPYEVLQPGGSVEYN
                     NEEAGAVWLQRFFATFPKSIWLNPEPESLWQYRQSISLIRQLAGDKMFPMTLEGLTRA
                     MRQLSK"
     gene            complement(4265..4858)
                     /locus_tag="VLN25_03035"
     CDS             complement(4265..4858)
                     /locus_tag="VLN25_03035"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HSQ09580.1"
                     /translation="MLVAQPVVLEGHGVRLEPLAREHHDGLVAAARDGELWNLWFTSV
                     PEPEQAHAYISAALAGREASHMLPWAVRDLAHDTIVGTTRYHDIVAAAERVEIGYTWY
                     ARRCQRSHVNTACKLLLLSHAFDELRCAVVGFRTDNFNFASQQAIAGLGAKKDGVIRH
                     HHPRRDGTVRDTVMFSILATEWPDVKRHLELRLARHR"
     gene            complement(4866..5711)
                     /locus_tag="VLN25_03040"
     CDS             complement(4866..5711)
                     /locus_tag="VLN25_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HSQ09581.1"
                     /translation="MSRFEGSTTYVATDDLKLAVNAALTLQRPLLIKGEPGTGKTMLA
                     EEVARALDMPLLAWHIKSTTKAQQGLYEYDAVSRLRDSQLGDARVKEIENYIVRGVLW
                     QAFTSEEQSVLLIDEIDKADIEFPNDLLRELDRMEFYVYETRQTVKARHRPLVVITSN
                     NEKELPDAFLRRCFFHYIRFPDKDTMQAIVDVHFPKLRKELLANALAVFFGVRELPGL
                     KKKPSTSELLDWLKLLVAEQIPPEALRSKDDKIAVPPLAGALLKNEQDMHLFERLVFM
                     ARSNR"
BASE COUNT         1089 a         1912 c         1941 g         1075 t
ORIGIN      
        1 gtgctgaagg ccgcgctgat ggacgcggcg ttctttgcct cgagtgcgca ccccgcgcgc
       61 aagctgatcg accgtatcgc aggaacgtcg gcgggttggg aaccctacgg tgacgacaac
      121 cagaagtacc tgaaggaagt cgaacgcgtc atcggcgaga tcctgaagaa attcgagacc
      181 gatctttccg tcttcgagaa ggtctatacc gagttcgacc gcttcgtctc ggaaatcgat
      241 cccggggaca gcgatccggt ctcccgcgcc aagaaagcgc tcgaggcggc cgagaagcac
      301 gagatcctca cgatcaacac aacgatccag gtgcggcgtg cattcgagcg tgtcgacctc
      361 gagccttaca tcaaggagtt cctgctgggg ccctgggtca aggtgctggt ggctgcgacg
      421 gtgcgcagcg agacccagcc tggcttcgcc aagtcattcc gcgacgtgat ccacgaactg
      481 gtctggtcgg tacagcccaa ggccagcacc gaagaccgca acaagctggt caagatgatt
      541 ccaaccatgg tgcgggtgct gcgcgacggc ctggcgctgg tcaaccagtc cgagcgcgac
      601 cgtgaccagt tcttcacctc actgatggaa tcgcacgcga tggcggtcaa gccggtcgac
      661 caggcaacgt acatcaagtc gagcctgctc acgtcggagt tgcgcgccaa gatcgacggc
      721 atgcagctgt caggcatctt ccccgtaacc accgtgcccg gcgggatccg cgtgtcgacc
      781 ggactgatca tgcgcgcggc acaggaacac cacgcggaca tccagatgcc ggacccactg
      841 accgacatcg cccgactcga tccggtcgag gacgcccggg tgcaggagga aatcggtcgc
      901 tggcagcgcg gcaactggtt caagatgtgg aacggcatgg aattcgtgcg cgtcaagctg
      961 cgctggatga gcccgttgcg gaccttgttc ctgtttgccg gcgagcatga ccagaaggcg
     1021 cacgtgctgt cgtcggacat gctgaagtcc tacctggcac gcaagtacat cgaaccgctc
     1081 gaaagcgttc cgctgaccaa gcgcgcggtt gacgcggtcg tcgccgactt cgagagcgca
     1141 ccggaccgag cgaccaatct ggccaagcgc tacaaggcgt cgcagccggc ggcatgaggc
     1201 gcgccgcctg aatcccaagg agggtgccgc ggcactgaag gccgttgcgt cttcgcaggc
     1261 gacggagcgg catgcgccga ccccggtgcc gagcggccgc gtgaccggcg gctatgtgct
     1321 caagcaactg gtcgccgacg gcatgctcgc gaccagcgat gccgagcgct tcaagatctc
     1381 cggagggcgc gaccgcgcag gtcatccgct ggtgaatctc gcggagatga actaccggac
     1441 gccgaatgcc gaacgcaagc tgctcgacct cgaggcgttg acgcagtggc tggcgcagaa
     1501 gtcgtcgctg ccttactttc acattgatcc gctgcgcgtc gacttcaccc gcgtcgtcga
     1561 cgtgatgtcg tcgtcgtatg cgacgaccta ctcgatcctg ccggtggcca tgggcggcga
     1621 cgaggtcacg atcgcgacct gcgagccctt ccagacggga tgggaacgcg agatcgagca
     1681 gatcacaaag aagaaggtca agcgcgtcgt ctcgaatccg ctcgacgtcg cgcgctacac
     1741 gaccgagttc tacaagctgg cgcagcaggt caggggcgcc gccaagtccg ggtctgcgcc
     1801 actggtgtcg agtttcgaac aactggtcga gctcggcggc aaggtcgagg ccaacgacgc
     1861 ccacgtcatt cacatcgtcg actggctgct gcagtatgca tttgaccagc gggcgagcga
     1921 tatccacctc gagcctcggc gcgagttcgg tgtcgcgcgc ttccgcatcg acggtgtgct
     1981 gcaccaggtc taccaggtgc ccggcggggt gatggcggcg atgaccagcc gcatcaagct
     2041 gctgggccgc atggacgtcg tcgagaagcg ccggccgcag gacggccgca tcaagaccaa
     2101 ggtcgcgacc ggaccgaatg caggccgcga ggtcgagatc cggctgtcga cgctgccgac
     2161 ggcatttggt gaaaagctgg tgatgcggat cttcgatccg gaagtcgtcg tgcgcagcta
     2221 cgccgaattg ggcttcaccc cggatgagac gaaggtctgg gaagacctga tccatcggcc
     2281 atcgggcatc gtgctggtga cggggccgac tggttccggc aagaccacca cgctgtactc
     2341 gacgctgaag caactggcga ccgatgaggt caacgtcagc accgtcgaag atccgatcga
     2401 aatggtcgat cccgcgctca accagatgca ggtccagcac ggcatcgacc tcggctttgc
     2461 cgacggcctg cgcgcattga tgcggcagga tccggacatc atcatggtgg gcgagatccg
     2521 cgacaaggaa accgccgaca tggcggtcca gtcagcgctg accggccacc tggtcttctc
     2581 gactctgcac accaacgacg ccgccgcgtc gattgcgcgc ctgctcgaac tcggcattcc
     2641 caactacctg gtcaatgcga cgctgatcgg catcctcgcg caacgactgg tgcgcacgct
     2701 gtgccagcac tgcaaggcgc cgggcgaccc gatcgaaccc atgctgtgga aggatctggt
     2761 ggcgccctgg cgctcgaacg tgccggagaa gatctatcac ccggttggct gcctcgagtg
     2821 tcgtcgcacc ggctacatgg gccgaaccgg attgatcgag atgctggtga tgagcgatgc
     2881 gatgcgcaag ctgatcggtg agggcgctga cctcgtccag ctgcgcggcc aggccgcgcg
     2941 cgacgggctg cgctcgctgc gcgtgtcggg cgcggagaag atcgccaagg gcatgaccac
     3001 gcttgacgag gtgctgaagg ccgcaccacc gttcaacaac tgacgggtct tacttcgaca
     3061 gctgacgcat tgcccgcgtc agtccctcga gagtcatcgg aaacatcttg tcgcccgcca
     3121 gctggcggat gagcgagatc gactggcggt actgccacag gctttccggt tccgggttca
     3181 gccagatcga cttcgggaac gtcgcgaaga accgctgcag ccagacggcg ccggcttctt
     3241 cgttgttgta ctcgaccgag ccgcccggct gcagcacttc gtagggactc attgtcgcgt
     3301 cgccgacgaa gacgagcttc cagtcgtgag ggtactttcg cagcacgtcc caagtggcga
     3361 agcgctcggt gtatcggcgt cggttgttgc gccagaggta ctcgtagacg cagttgtgaa
     3421 agtagtagaa ctcgaggtgc ttgaactcgc ccttcgcggc ggaaaagagt tcctcggtgc
     3481 gcttgatgtg ctcgtccatc gtgccgccga catcgagcag catcagcacc ttgacggtgt
     3541 tatggcgctc cggcacgagc ttcaggtcaa gccagccggc gtttttcgcg gtcgactcga
     3601 tcgtgtcgtg catgtcgagc tcgaggtcgg cgccctcgcg tgcgaagcgc cgcaaccggc
     3661 gcagtgcgac cttgatgttg cgcgtgccga tttcgaccga gtcatcgtag tcgcggaagc
     3721 cgcgcatctc ccagaccttg acggccgact tcccgccctt gctcggcccg ccgacgcgga
     3781 taccctcggg attcgcgccg ccgtgtccga acggcgaggt tccgccggat ccgatccagc
     3841 ggctgccacc atggtgcgcg ccgtcctgct cctcgaggcg cttcctgaac tcctccatca
     3901 gcttgtccca gccgagcttc tcgagcttcg ccatttcttc cgggctgaac gcgcgctcga
     3961 gcaccttcct gaaccactcg tccgggatct ccttcgtgaa atcaaccgag gtcgtcacgc
     4021 ccttgaagta ctcgccgaag gcccggtcga acttgtcgaa ctgtgtttca tccttgacga
     4081 gcgtggttcg cgacagatgg tagaactcgt cgaggagcga aagcccgccc tccgggctct
     4141 ggggtccgat cacgccctgc ttcagcgcct cgagcagcgt cagcagctcg ttgatcgaca
     4201 ccttgagctt ggcggcacgc aggtgaaaga agaagtcgat cagcatcgcg atagcggtgg
     4261 cgcgtcaacg gtggcgcgcc aggcgcagtt cgaggtggcg cttcacgtcc ggccactcgg
     4321 tggccaggat gctgaacatc acggtgtcgc gcactgtgcc gtcgcggcgc gggtggtgat
     4381 ggcggatcac gccgtccttc tttgctccga gtccggcgat cgcctgctgc gaggcgaagt
     4441 tgaagttgtc ggtgcggaac ccgaccaccg cgcagcgcaa ttcatcgaac gcatggctga
     4501 gcagcaacaa cttgcaggcc gtgttcacgt gcgacctctg gcagcggcgc gcgtaccacg
     4561 tgtagccgat ctcgacgcgt tcggccgcgg cgacgatgtc gtggtagcgc gtggtgccga
     4621 caatcgtgtc gtgtgcgagg tcgcgcaccg cccacggcag catgtggctg gcctcccggc
     4681 ccgccagcgc ggccgagatg taggcgtgag cctgctcggg ctcgggcacg gacgtgaacc
     4741 acaggttcca cagttcgccg tcgcgcgcgg ccgcgaccag tccgtcgtgg tgttcgcgcg
     4801 ccagcggctc gagccgcacg ccgtggcctt cgagcacgac cggctgggca acgagcatta
     4861 cagcctcagc ggttgctgcg ggccatgaag acgagccgtt cgaacagatg catgtcctgc
     4921 tcgtttttca gcagcgcacc ggccagcggc ggcaccgcga tcttgtcgtc cttcgatcgc
     4981 agcgcttccg gcgggatctg ttcggccacc agcagcttca gccagtcgag cagctccgat
     5041 gtcgacggct tcttcttgag gcccggcaac tcgcgcacgc cgaagaacac cgccagcgca
     5101 ttggcgagga gctccttgcg cagcttcgga aagtggacat cgacgatcgc ctgcatcgtg
     5161 tccttgtccg gaaaccggat gtagtgaaag aagcagcgac gcaggaatgc atccggcagt
     5221 tccttctcgt tgttcgatgt gatcaccacc agcggacgat gccttgcctt gaccgtctgc
     5281 cgcgtctcgt acacatagaa ctccatgcgg tcgagctcgc gcagcaggtc attggggaat
     5341 tcgatgtcgg ccttgtcgat ctcgtcgatc agcagcaccg attgttcctc cgacgtgaac
     5401 gcctgccaaa gcacgccacg gacgatgtag ttctcgattt ccttgacgcg tgcatcacct
     5461 agttgcgagt cgcgcagccg gctgaccgcg tcgtactcat acaggccctg ctgcgccttg
     5521 gtggtggact tgatgtgcca ggcaagcaac ggcatgtcga gcgcgcgcgc cacttcctcg
     5581 gccagcatgg tcttgcccgt acccggttcg cctttgatca gcagcggtcg ctgcagcgtc
     5641 agcgcggcgt tgaccgccag cttgaggtcg tcggtggcta cgtaggtcgt tgagccttcg
     5701 aaacgtgaca tggacggatg cggaagcggc tgagaggagc cgatcgagta tacgcaacgg
     5761 cgctccgaaa cctgcgcact ggacggtgcc gcgacccggt ggtcggactc gattacaatc
     5821 gcgcgctgcg tgccggggag ggcgcggccg ggcgagtggg ttcacgacac tacgatcccg
     5881 ggatttgctt caacatcacc tacggatagt gcggatgaag ttggaccgtt tgctgctcgt
     5941 cgccgttgcc gcgacactcc cgctgctggc ggcggcgcag gacaagaagg ccgagtcgcc
     6001 ggcaccggcc gcaacca
//
LOCUS       DATIRT010000126         2933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_605430, whole genome shotgun sequence.
ACCESSION   DATIRT010000126 DATIRT010000000
VERSION     DATIRT010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605430"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /gene="prmB"
                     /locus_tag="VLN25_03045"
     CDS             <1..690
                     /gene="prmB"
                     /locus_tag="VLN25_03045"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: protein motif:HMM:TIGR03533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HSQ09582.1"
                     /translation="IHELLQAIDRRVKKRLPAAYLLNEAWLRGYKFYVDERVIVPRSF
                     IGELLDDGLSPWVQDRAAVTEVLDMCTGSGCLAIMAAVVFPNAKVDAADTSADALQVA
                     RRNIADYQFEQRITPVKSDLFSALGTKRYDIILCNPPYVTDAAMAKLPVEYTHEPKLA
                     LAGGADGMNLVKTLVRQARGHLKRGGVLIVEVGDGRPAADKIFSDLPLTWLTTSAGDD
                     MVFLVRQEELA"
     gene            846..2738
                     /locus_tag="VLN25_03050"
     CDS             846..2738
                     /locus_tag="VLN25_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016473953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSQ09583.1"
                     /translation="MLRLIDVALARGARTLYRGVSLIARPGERVGLVGANGCGKSTLF
                     AAILGDLSLEAGSMEVPPPERIAHVAQDIDVSGQRAIDYVLAGHAPLTAARAELAAAE
                     ASHDEMRLAHAHVAMADLNEGSIVAKAATVMHGLGFATSDGDRPVSAFSGGWRNRLAL
                     ARALLRPADLLLLDEPTNHLDLDSVVWLESWLRRQPATVLVISHDREFLDRIAETIWH
                     VDNNAIRGYAGDYSAFELAWLEQQKQQDAAAKAYSRTAAHLQQFVDRFRAQATKARQA
                     QSRLKMLERLTVIEPTRARREWRFEFPEPVRVPERLLDAEDMSLGYGDRTVLGDVTLT
                     VRAGDRIGILGVNGAGKSTLVKAIAQELAPSAGELRRGQGLAIGYFAQHQLDQLRPDE
                     TPLAHLRRLAPAAREQELRNFLGNYRFSGEMATSLLGPMSGGEKARCALALIAWQRPN
                     LLLLDEPTNHLDMETREALTMALSSYDGALLLVSHDRHLLRATCDELWLVHDGRVAPF
                     DGDLDDYSALVLASRRDRNDALTDAEGPNRREQRKQEAAARQRLAANRRPLQTKLKRL
                     EDELARISDELRELDAQLADPDFYHSGNDDAVAKTLKRRGELAGRVEELEANWIALTS
                     ELEAIA"
     gene            2809..>2933
                     /locus_tag="VLN25_03055"
     CDS             2809..>2933
                     /locus_tag="VLN25_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HSQ09584.1"
                     /translation="MNRPIAAPLAGLRILDLTRLLPGPAATMHLADFGADVIKIE"
BASE COUNT          494 a          990 c          969 g          480 t
ORIGIN      
        1 atccacgaac tgctgcaagc catcgaccgt cgcgtgaaga agcgcttgcc ggcggcgtac
       61 ctgctcaacg aagcctggct gcgaggctac aagttctatg tcgacgagcg cgtgatcgtg
      121 ccgcgctcgt tcatcggcga actgctcgac gatggcctgt cgccctgggt gcaggatcgc
      181 gccgccgtca ccgaggtgct cgacatgtgc accggttcgg gctgcctcgc gatcatggct
      241 gccgttgttt ttccgaacgc caaggtggat gcggccgata cctcggccga cgcactgcag
      301 gtggcgcgac gcaatatcgc cgactaccag ttcgaacagc gcatcacgcc ggtcaagtcg
      361 gacctgttct ccgcgctcgg caccaagcgc tacgacatca ttctgtgcaa tccaccgtac
      421 gtcaccgacg ccgccatggc caagctgccg gtggagtaca cgcatgagcc caagctggcg
      481 ctggcgggcg gtgccgacgg catgaacctg gtgaagacgc tggtgcggca ggcccgcggt
      541 cacctgaagc gcggcggcgt gctgatcgtg gaagtcggcg acggtcgtcc ggcggcggac
      601 aagatattca gcgatcttcc gctgacctgg ctcacgacca gcgccggcga tgacatggtg
      661 ttcctggtcc gccaggaaga acttgcatga ctgggacgct gctcgtgtac gcgaccgcat
      721 tgcggtcgca cgtgcattcg cagcgactag tcgggcgcgc tcccttcgga gcggccgcgc
      781 gtgcgcgcgc agaatgaggg cacggggctc ctgcgcttcg ctcgccacac aacactgcga
      841 ttgacatgct taggctgatc gacgttgcgc tggcacgcgg tgcgcgcacg ctgtaccggg
      901 gcgtttcact gatcgcgcgc cccggtgagc gcgtcggcct cgtcggcgcc aatgggtgcg
      961 gcaagtcgac actttttgcg gcaatccttg gcgacctgag cctcgaagcc ggctcgatgg
     1021 aggtgccacc gcctgaacgc atcgcgcacg tggctcagga cattgatgtc agcggccagc
     1081 gcgcgatcga ctacgtcctg gccggccacg cgccgctgac ggcggcgcgg gccgaactgg
     1141 ctgcggccga agcaagccac gacgagatgc gactggcgca cgcgcatgta gcaatggcgg
     1201 atctcaatga agggtcgatc gtcgcaaagg ccgccaccgt gatgcacggt ctgggctttg
     1261 ccacctcgga tggtgaccgc ccggtgtcgg cattctccgg cggctggcgc aaccgtctcg
     1321 cgctggcgcg tgcgctgctg cggccggcgg atctcctgct gctcgacgag ccgaccaatc
     1381 acctcgacct ggattccgtg gtctggctcg aatcctggct gcgccgccag ccggcgaccg
     1441 tgctcgtcat ctcgcatgac cgcgagttcc tcgaccgcat cgccgagacg atctggcacg
     1501 ttgacaacaa cgcgatccga ggctacgcgg gtgactactc ggcgttcgag ctggcctggc
     1561 tcgagcagca gaagcagcaa gatgccgcgg ccaaggccta ctcgcgcacg gcggcacacc
     1621 tgcagcagtt cgtcgaccgc ttccgcgccc aggcgacaaa ggcgcgtcag gcgcagagtc
     1681 gactgaagat gctggagcgg ctgaccgtca tcgagccgac gcgcgcgcgg cgcgagtggc
     1741 gcttcgagtt tcctgaaccg gttcgcgtcc ccgagcgact gctcgatgcg gaagacatgt
     1801 cgctcggcta tggcgaccgc acagtgttgg gcgatgtcac gctgaccgtc cgtgccggtg
     1861 accgcatcgg aatccttggc gtcaacggcg ccggcaagtc gacgctcgtc aaggcgatcg
     1921 cacaagagct cgctccctcg gccggcgaac tgcggcgcgg ccagggcctg gcgatcggct
     1981 actttgcgca gcaccaactc gaccagctgc gccctgatga aactccgctg gcgcacctgc
     2041 gccggctggc gccggcggcg cgtgaacagg agctgcgcaa ctttctcggc aactaccgat
     2101 tttccggcga gatggccaca tcgctgctgg gcccgatgtc gggtggcgag aaggcacgct
     2161 gtgcgctggc cctcatcgcc tggcagcgcc ccaacctgct gctgctggac gagccaacca
     2221 atcacctcga catggagacg cgcgaggcgt tgaccatggc cttgtcgtcc tacgacggtg
     2281 cgctgctgct cgtgtcgcac gaccgccatc tgctgcgtgc cacctgcgac gaactgtggc
     2341 tggtgcacga cggccgcgtc gccccgttcg atggcgacct cgacgattac agcgccctcg
     2401 tcctggccag ccgccgcgac cgaaacgacg cgttgaccga tgccgagggt ccgaaccggc
     2461 gcgagcaacg caagcaggaa gccgcggcgc gccagcgcct tgccgcgaat cgccgtccgc
     2521 tgcagacaaa gctgaaacgt cttgaagacg agcttgcgcg gatctccgat gaactgcgcg
     2581 aactcgatgc ccagctggcc gacccggact tctatcactc cggcaatgat gatgctgttg
     2641 cgaagacgct gaagcgtcga ggggaactgg ccggccgggt cgaagagctc gaagcgaact
     2701 ggatcgccct cacctcggaa ctcgaggcca tcgcatgagc ggcgcccggc cgcttcgaag
     2761 gcgctcgcgt cccccgcggg gggaccgccc gcaggacgag agggcaactt gaaccgtccc
     2821 atcgcagcac cgcttgccgg cctgcgcatt ctcgacctga cgcggttgct gcccggaccg
     2881 gctgcgacga tgcacctggc cgactttggc gcggacgtca tcaagatcga gga
//
LOCUS       DATIRT010000127         9679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_900635, whole genome shotgun sequence.
ACCESSION   DATIRT010000127 DATIRT010000000
VERSION     DATIRT010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9679
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900635"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..564)
                     /locus_tag="VLN25_03060"
     CDS             complement(13..564)
                     /locus_tag="VLN25_03060"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HSQ09585.1"
                     /translation="MPRKFFRKVMPNVDKVREMRVLSMFGDSLFHPALWHLNRRSAAD
                     GVAVGLFCGLIPGPLQMLGAGIACVLFRVNLPVSLVTTFYTNPLTIVPLYLVAYQIGS
                     FVLGASGSKPVTRPPDWEWSQPFGSAEAIGHWMIGLGPPLALGVLLLACLLAILGYLI
                     VRGLWSVHLRRAWHARRRRTTPS"
     gene            681..1484
                     /locus_tag="VLN25_03065"
     CDS             681..1484
                     /locus_tag="VLN25_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HSQ09586.1"
                     /translation="MWIDTHCHLDADEFDRDRDEVVARAREAGVGMMVIPTGHVDERD
                     EVRAIAHRHGFAYALGLHPLWLAEAVEDDIERLREAVQESLADPRFVAVGEIGIDLAE
                     PGVDPARQEWFFREQLRIARKAEVPVIVHVRQSADLLLKHLRRIDVPGGIIHAFNGSA
                     QQASQFVTRGFKLGFGGALTYSGSQRIRRHAAELAVDAWVLETDAPYIAPSWRRTPER
                     VERTEPCDLRRIAGELAQLRGTDLATLARQNRANALAALPRLGPLLPSA"
     gene            1488..3959
                     /locus_tag="VLN25_03070"
     CDS             1488..3959
                     /locus_tag="VLN25_03070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HSQ09587.1"
                     /translation="MLIAPLAAFVLGVCALQLQAELSSLPILLVAAIGGAVLLVFGVR
                     SRSRRMALMTFMCSGALLAGFAYAGARAHVRLADFLPFADEGRDVIVTGVVSSLPVKL
                     ERGVRFEFDVESHRVESHGADVAVPRRLLLGWYAAGEPVRPGERWRFEVRLRRPHGAM
                     NPGGFDFEAWMLERNLRASGYVRTPAARPPERLQSMVWSPGIAVERARAWLRDRLLPH
                     LQSERYGGVVLALVLGDQRAIADTDWTLFNRTGITHLVSISGLHITMIAALAGGMTGA
                     FWRRSPWLLRRAPVQTAAIVAGVAAALLYALLAGWGIPAQRTVLMLVCVAAAWTARAR
                     VTPGVALALAAAAVCVFDPWAVTAAGFWLSFGAFAAIIWVVQGRPRRDGSLAMNAVVT
                     ATRIQIAVTLALVPATVVLFQQISVVAPLANAIAIPLISWIVTPLALVGAGIAALPDA
                     LWPLASFFLGIASRVFAGLAAVLAAVSAPPWAALAVSAAPWWVTAFALAGVAWLLAPP
                     GWPARGLGAIALVPLFVWPVERPLAGGLWITALDVGQGSAVVLETREHAWLYDTGPRY
                     SQDSDAGERVILPFLRSRGIQSLDGVVVSHLDLDHSGGAASLLRGVDVRRRVSSVAPG
                     HASLGGADVERCVAGMTMESGDMSLRVLHPPDVDYERRLPTNAMSCVVLARVGGVAVL
                     LTGDLPARGETELILREPELRANWMMAPHHGSHSSSSEMLLESVAPAVAIAQAGYRNR
                     FGHPDPRVVARYAARGIELIRTDHAGAAQWRYAPGESVEFRSWRATAVRYWHNRPAAG
                     RTGAATEPDEALEEAEVREPLFGMP"
     gene            4047..5468
                     /locus_tag="VLN25_03075"
     CDS             4047..5468
                     /locus_tag="VLN25_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HSQ09588.1"
                     /translation="MSKAFDEMYGEEAQVREHYREFGRWLAEQSGETMRNKRAEADLV
                     FRRVGITFAVYGEQDEEGNGTERLIPFDVVPRIIPAAEWRQLEAGLRQRVRALNQYIH
                     DVYHDQEIVRAGRIPADEIFRNSQYRPEMHGVDVASNIYAHIAGIDVVRAGAGEFYVL
                     EDNLRVPSGVSYMLENRKMMMRLFPELFGRNRVAPVAHYPDLLLENLRSVAPQGVDDP
                     LVVLLTPGMHNSAYFEHAFLAQQMGIELVEGQDLFVQDNNVYMRTTRGPLRVDVIYRR
                     IDDDFIDPLTFRPDSQLGVPGLLSVYRAGRVTLANAIGTGIADDKSIYAYVPDMIDFY
                     LGEKPILNNVPTYRCRRPDDLEYTLAHMSELVVKEVHGAGGYGMLVGPAAARSEIEDF
                     SQRVRANPTNYIAQPTLALSTCPTYVDSGIAPRHIDLRPFVLSGSNVTMVPGGLCRVA
                     LREGSLVVNSSQGGGTKDTWVLE"
     gene            5465..6325
                     /locus_tag="VLN25_03080"
     CDS             5465..6325
                     /locus_tag="VLN25_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09589.1"
                     /translation="MMKRTSDHPVGSDAAGGEPTARRVTDQIDPYALIDRAERLLNTD
                     PHDAVMFALRAQSVARARGAEALMARAHYVAGAALTQTGDWSGAIRQLSHAEHIYRAQ
                     NDITSQCQVIMQLAAACSELGEYPEALDGFAQAERLARQHNDHAAVRKALVQTSSVHT
                     VLGDFSAARECLRSALELPSGTAADEGMVVLQFGLVDVNEGLKAALAGDAATVSRRMA
                     EGEEHFDVALELLSGTTDRSTTIAARTQRGAARCWLGDFAGGLADLEEAGVEAARGGF
                     RLREVQARLE"
     gene            6431..7162
                     /locus_tag="VLN25_03085"
     CDS             6431..7162
                     /locus_tag="VLN25_03085"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HSQ09590.1"
                     /translation="MHERLSALYEARTDFQRALFHHKRFVEVKTRLDGKLSTQRARLH
                     EARAELVKLRTQTAALHARTQDLEKSRSRLERLALEDELTGLANRRAFTKELENWCGR
                     ARAGQLRFGLVVADIDGFKQINDSYSHVTGDVVLRQLGNVIRANLRDMDFAARLGGDE
                     FALLLADSEGGAARTVVERILQAVRNMPWGQLGEGMAVTLSVGLCESADFGDSSELVR
                     RADEALYGAKDAGRDRMHLWSADRA"
     gene            7155..8153
                     /locus_tag="VLN25_03090"
     CDS             7155..8153
                     /locus_tag="VLN25_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HSQ09591.1"
                     /translation="MLSRTADHLYWMARYIERAENTARMLDVNLTTSLLPQSVQAAES
                     GWRAMLNISELLPQFDARYPTLSPREVIDFMVRDPENSSSIYCCLQAARENARAVRGS
                     LTTEVWETHNDTWLGLQPLLRERRLERDPQEFFEWVKFRSHLSRGVTIGTMLKDEAFY
                     FIRLGTFLERADNTARLLDVKYHGVVQPGTLYTNARGEDANSSGEDANEQHLDFYHWT
                     AVLRSVSAFEIYRKVYRDVITPARIAALLILRGDMPRSLLSCVNEVSLNLEMVRNSRS
                     EETERRAGLLRAELRYGRIEDILARGIHEFLTSFLERVNDLGGRISQDFLVPLVAH"
     gene            8178..9017
                     /locus_tag="VLN25_03095"
     CDS             8178..9017
                     /locus_tag="VLN25_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HSQ09592.1"
                     /translation="MRYCIEHLTDYRYTHVAVSTQQTLRLSPRIEAHQRVFNWKIRVP
                     GTLSSANDAYGNLTHMHTLHSKHDRVCIEVQGEVELDALNEGRLAERGGLPPQVFVAV
                     TPLTVADERIADFARIQLKRATAAGVLEFAQAVRSAIDYEAGTTHVRTTAAEALALGR
                     GVCQDHAHVFIAGCRAAGIPARYVSGYYYSERHASHVASHAWADVWVSDGWTSVDITH
                     ACFASDALCRLAIGRDYDSASPVRGVRAGGGEEAMKVALTVRRVAQTADRTPAARPEE
                     VTA"
     gene            9014..>9679
                     /locus_tag="VLN25_03100"
     CDS             9014..>9679
                     /locus_tag="VLN25_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HSQ09593.1"
                     /translation="MTYCIGVQLDDGLVFLSDSRTNAGVDHISTFRKMTVFEHPGERL
                     MVLLSAGNLAVTQAVRQIVCERVGPHDDSVWNAKTLFEAVRVVGEAIREVHQRDGQAL
                     KDAGMEFNCSFIFGGQIRGERMRLFQLYSAGNFIEATPENAYFQIGEAKYGKPIIDRV
                     ISPSTSLEEAAKCALISMDSTLRSNISVGLPLDLLVYEQGALRVTKFVQIDHHNQYMQ
                     MIRN"
BASE COUNT         1568 a         3125 c         3329 g         1657 t
ORIGIN      
        1 agcctgatcg gatcatgatg gcgtcgtgcg gcgacggcgc gcatgccacg cgcgacgcaa
       61 gtgcacgctc cagagccccc ggacaatgag gtagccgagg atcgccagca ggcaagcaag
      121 cagcagcact cccagtgcca gcggcgggcc gaggccgatc atccagtgtc cgatggcttc
      181 tgcagatccg aacggttgcg accattccca gtcgggcggc cgggtcaccg gtttcgatcc
      241 cgacgctccc agcacgaacg agccgatctg gtaggcgacc aggtacagcg ggacgatggt
      301 cagcggattc gtataaaagg tggtgaccag cgataccggc aggttgacgc ggaacagcac
      361 gcaggcgatg ccggcaccga gcatctgcag cggtcccggg atcagcccgc agaacagccc
      421 taccgcgacg ccgtccgccg ctgaacggcg attcaggtgc cataaggccg gatggaacag
      481 gctgtcgccg aacatcgaca ggacgcgcat ttcgcgcacc ttgtcgacat tgggcatcac
      541 cttgcggaag aacttgcgcg gcatgcaggg agtctagggc ctgttcacgc caaagccggg
      601 agtgcgaagg ccaatggcgg gaggcagtgc gaggcgccgc gagcgccgtt ggtgcacgca
      661 gggtgcttac actccttccg atgtggatcg acacccattg ccatcttgac gcggacgagt
      721 tcgatcgcga ccgcgacgaa gtggtcgcgc gggcgcgcga ggccggggtc ggcatgatgg
      781 tcattccgac cggacacgtg gacgagcgcg acgaagtgcg cgcgattgcg caccgccatg
      841 gcttcgccta cgcgctggga ttgcatccgc tgtggcttgc agaggctgtg gaggacgata
      901 tcgaacgact gcgcgaggcc gtacaggagt cgctcgccga cccgcgcttt gtcgccgtgg
      961 gcgagatcgg catcgacctc gccgagcccg gcgtcgatcc ggcacgacag gaatggttct
     1021 tccgcgagca gttgcgcatc gcgcgcaagg ccgaagtgcc cgtcatcgtg cacgtgcggc
     1081 agtcggcgga cctgctgctt aagcatctgc ggcgcatcga cgtgccgggc ggcatcatcc
     1141 atgccttcaa cggcagtgct cagcaggcca gccagttcgt gacgcggggt ttcaagctcg
     1201 gcttcggcgg ggcgctgacc tacagcggat cgcagcgcat ccggcgtcat gcggccgagc
     1261 tggcggtcga cgcgtgggtg ctcgaaacgg atgcgccgta tatcgccccg tcctggcgac
     1321 gaacaccgga acgcgtcgag cggaccgagc cctgcgatct gcggcgcatc gccggggaac
     1381 tggctcagtt gcgcgggacc gacctcgcca ccctggcacg tcagaaccgc gcgaatgcgc
     1441 tggcggcgct gcctcggttg gggccgctcc tgccctctgc atagccaatg ctcatcgcac
     1501 cgctagccgc gttcgtgctc ggcgtgtgcg cgctgcaact gcaagcggag ttgtcctcgc
     1561 tgccgatatt gctggtcgcg gcgatcggcg gggccgtcct gctcgtgttc ggcgtgcgct
     1621 cccgatcgag gcgcatggcc ctgatgacgt tcatgtgcag cggtgcgttg ctcgcgggct
     1681 ttgcgtatgc cggtgcgcgc gcgcatgtac gactggcgga cttcctcccg ttcgccgacg
     1741 agggccgcga tgtcatcgtg accggcgtcg tgtcgagcct gcccgtcaaa ctggaacgcg
     1801 gcgttcgctt tgaatttgac gttgagtcac acagggtcga atcacacggg gccgacgttg
     1861 ccgtgccaag acgcctgctg ctcggctggt atgccgcggg cgaaccggtt cggcccggag
     1921 agcgctggcg cttcgaggtg cgcctgcgtc ggccacacgg ggcgatgaat cccggcgggt
     1981 tcgacttcga agcgtggatg ctcgagcgca acctgcgcgc gagcggctac gtacgcacgc
     2041 cggccgcgcg gccgcccgag cggctgcagt cgatggtgtg gtcccccggc atcgccgtcg
     2101 agcgggcacg cgcctggctt cgagatcgcc tgttgccgca tctgcagtct gagcgctacg
     2161 gcggcgtcgt gctggccctg gtgctgggtg accagcgcgc catcgccgat accgactgga
     2221 cgctcttcaa tcgcaccggc atcacccacc tggtcagcat ctcggggctg cacatcacaa
     2281 tgatcgccgc acttgccggt ggcatgaccg gagcgttctg gcgccgctcg ccctggctgc
     2341 tgcggcgtgc gccggtccag acggcggcca tcgtcgccgg tgtcgccgct gcactgctgt
     2401 atgcactgct cgcggggtgg ggcataccgg cccaacggac ggttctgatg ctcgtgtgtg
     2461 tcgccgccgc gtggactgca cgtgcccgcg tgacaccggg tgtggcgctt gcactcgcgg
     2521 cggcagcggt gtgcgtattc gatccctggg ctgtgacggc ggccgggttc tggctttcat
     2581 tcggcgcctt cgcggccatc atctgggtcg tgcagggacg tccacgccgc gacggttctt
     2641 tggcgatgaa cgcggtcgtt accgcaacgc gcattcagat tgcagtcacg ctggcgctgg
     2701 tgccggcgac agtcgtgctg ttccagcaga tttcggtggt ggcaccgctg gccaacgcga
     2761 tcgcgatccc gctgatcagc tggatcgtga ccccgctcgc gctggtcggc gccggcatcg
     2821 ccgcgcttcc cgatgcgtta tggccgctgg cctcgttctt cctcgggatc gccagtaggg
     2881 tgttcgcggg ccttgctgcg gtgctcgccg ctgtgtccgc gccgccctgg gctgcattgg
     2941 ccgtgtccgc agctccctgg tgggtcaccg cgttcgcact cgccggagtt gcctggctgc
     3001 tggcgccgcc gggctggccg gcgagagggc tcggcgcgat tgcgctcgtc cccttgtttg
     3061 tatggccggt cgaacgaccg ctcgcgggcg ggctctggat tactgcactg gatgtcggcc
     3121 agggatcggc cgtcgtgctg gaaacccgag agcacgcgtg gctgtacgac accgggccgc
     3181 gttactcgca ggactccgat gccggggagc gcgtgattct gcccttcctg cgtagccgcg
     3241 gcatccagtc gctcgatggt gtggtcgtgt cgcatctcga cctggatcac tccgggggtg
     3301 ccgcctcgct gctgcgcggc gtcgacgtgc gtcgccgggt gagttcggtt gcgccggggc
     3361 atgcgtcgct gggcggtgcc gacgtcgaac gttgcgtcgc cggcatgacg atggagtccg
     3421 gcgacatgag cctgcgggtg ctgcatccgc ccgacgtcga ctacgaacgg cggctcccga
     3481 cgaatgcgat gagctgtgtc gttctcgcgc gggtgggcgg cgtggcggtc ctgctgaccg
     3541 gtgacctccc ggcacgcggt gagacagaac tgatcctgcg cgagccggaa ctgcgggcga
     3601 actggatgat ggcgccacac cacggcagcc actcgtcgtc gagcgagatg ttgctcgagt
     3661 cggttgctcc ggcggtcgcc atcgcgcagg cgggctatcg caaccgcttc ggtcaccccg
     3721 atccgcgggt ggtcgcgcgt tacgccgcgc gtggcatcga gctgatccgc accgatcacg
     3781 cgggcgcggc ccagtggcgc tacgcgccag gagaatcggt cgaattccgg tcatggcgcg
     3841 ccaccgcggt gcgctactgg cataaccgcc ccgccgcggg gcgcaccggt gcggcgacgg
     3901 agccggatga ggctctcgaa gaagccgaag tgcgggaacc gcttttcggg atgccataga
     3961 cccggagact ggtgtacatc ttgctagact ggatcggcac cgcgtcgggg ggcgccggac
     4021 aataacgaga ctcactcagg ccgcggatga gcaaggcgtt tgacgagatg tacggggagg
     4081 aagcccaggt tcgcgagcac tatcgcgaat tcgggcgctg gctcgccgag cagagcggcg
     4141 agacgatgcg caacaagcgc gccgaagccg acctcgtctt ccgtcgcgtc ggcatcacgt
     4201 ttgccgtcta cggcgagcag gatgaagagg gcaacgggac ggaacgactg attccgttcg
     4261 atgtcgtccc ccgcatcatc ccggcggccg agtggcgcca gcttgaagcg ggcctgcgcc
     4321 agcgcgtgcg cgcgctgaac cagtacatcc acgacgtcta ccatgatcag gagatcgtgc
     4381 gggcggggcg cattccggcc gacgagatct tccgcaattc ccagtaccgg ccggagatgc
     4441 acggtgtcga cgtggcgagc aacatctacg cccacatcgc cggcatcgat gtcgtacgtg
     4501 ccggcgccgg tgagttctac gtgctcgagg acaacctgcg cgttcccagc ggcgtgtcgt
     4561 acatgctcga gaaccgcaag atgatgatgc ggctttttcc cgagcttttc gggcgcaatc
     4621 gggtcgcgcc ggtcgcgcat tatcccgatc tgctgctgga aaacctgcgc tcggtggcgc
     4681 cgcagggcgt cgacgatcct ctggtcgtgc tgctgacgcc aggcatgcac aactcggcgt
     4741 acttcgaaca tgcgttcctg gcgcagcaga tgggcatcga actggtcgag ggtcaggatc
     4801 tcttcgtgca ggacaacaat gtctacatgc ggacgacgcg tgggcccctg cgggtagacg
     4861 tgatctatcg ccgcatcgac gacgatttca tcgatccgct gacattccgt cctgattcgc
     4921 aactcggcgt gcccggattg ctgtccgtat atcgcgcggg ccgggtcacg ctcgccaacg
     4981 ccatcggcac cggcatcgcc gacgacaagt cgatctacgc ctacgtgccg gacatgatcg
     5041 acttctacct tggcgagaaa ccgatcctca acaacgtccc gacgtaccgt tgccggcggc
     5101 cggacgacct cgagtacacg ctggcgcaca tgtccgagct ggtcgtgaag gaggtccacg
     5161 gcgcaggcgg ctacgggatg ctggttggac ccgcggcggc gcgcagcgag atcgaggact
     5221 tcagccaacg cgtgcgtgcc aatcctacga actacattgc ccagccgacc ctggccctgt
     5281 caacctgccc gacctatgta gacagtggca ttgccccgcg ccacatcgac ctgcgtccgt
     5341 tcgtgctgtc gggatccaac gtcaccatgg ttccgggcgg tttgtgccgg gtcgcgctgc
     5401 gcgaaggatc gctcgtcgtg aactcgtcgc agggtggcgg cacgaaagac acctgggtac
     5461 tcgaatgatg aagcgcactt ctgaccaccc cgtaggcagc gatgcggccg gcggcgagcc
     5521 gacggcccgg cgcgtcaccg accagatcga tccctacgcg ctgatcgacc gggccgaacg
     5581 cctgctgaac accgatccgc acgatgcggt gatgtttgcg ttgcgcgcgc agagcgtggc
     5641 acgggcgcgc ggggccgaag cgctgatggc ccgtgcgcac tacgttgccg gcgccgcgct
     5701 gacgcagacc ggcgactggt ccggggccat tcggcagctg tcgcacgcgg aacatatcta
     5761 tcgcgcccag aacgacatca cgtcgcagtg ccaggtcatc atgcagctcg ccgcggcctg
     5821 cagcgagctc ggcgagtacc cggaggcact cgacggcttc gctcaggcgg aacgcctcgc
     5881 gcgccagcac aacgaccacg ccgccgtgcg caaggcactg gtgcagacat cgagcgtgca
     5941 cacggtcctc ggggatttca gcgcggcgcg cgagtgcctg cgatcggcgc tcgagctgcc
     6001 gtccggaacc gcggccgacg agggcatggt ggtactgcag ttcggcctcg tggatgtcaa
     6061 cgaaggcctg aaggcggcgc tcgccggaga tgctgcaacc gtgtcgcgtc gcatggccga
     6121 aggcgaggag cacttcgacg tggcgctcga actgctgtcg ggcacgacgg accggtcgac
     6181 gacgatcgcg gcccgcaccc agaggggtgc ggcgcgctgc tggctcgggg acttcgccgg
     6241 agggctggca gatctcgaag aggccggcgt cgaggcggca cgcggcggtt tccggttgcg
     6301 tgaagtgcag gcgcggctcg agtagggcta tcacctcgtt gctggcggcc gcgaggccga
     6361 cgggctgtcg ctgctgcgcc ctgccttcct ccaggctgag gaactcggcg acctgcgtcg
     6421 gccggccgac atgcacgagc gactgtcagc cctgtacgag gcacgcacgg attttcagcg
     6481 cgcgcttttc caccacaagc gcttcgtcga ggtgaagacg cggctggacg gcaagctgtc
     6541 gacgcagcgc gcgcgcctgc acgaggcgcg tgccgagttg gtgaagctgc gcacccagac
     6601 ggccgcgctg cacgcacgta cacaggatct cgagaagtcc cgctcgcgcc tggagcggct
     6661 cgcccttgag gacgaactga cgggcctggc gaaccgacgc gcatttacca aggaactgga
     6721 gaactggtgc gggcgcgccc gcgcgggcca gcttaggttc ggcctggtcg tggccgacat
     6781 cgatggcttc aagcagatca acgacagcta ctcccacgtg accggtgacg tcgtgctgag
     6841 gcagcttggc aacgtgatcc gggccaattt gcgcgacatg gacttcgcgg cgcgactggg
     6901 tggtgacgag ttcgcgctgc tgctggccga cagcgagggc ggtgcggcgc gtaccgtggt
     6961 cgagcgcatc ctgcaggcgg tgcgcaacat gccctggggc cagctcggcg agggcatggc
     7021 cgtgacgctc agcgtcgggc tatgcgagtc cgcggacttc ggcgattcga gcgaactcgt
     7081 gcgacgcgcc gacgaggcgc tgtacggcgc caaggacgcc ggccgcgatc ggatgcatct
     7141 gtggagcgcc gaccgtgctt agccgtacgg ccgaccacct gtactggatg gcgcggtata
     7201 tcgagcgcgc cgagaacacg gcacgcatgc tggacgtgaa tctgacgacc agcctgctgc
     7261 cacagtcggt acaggccgcc gagagcggct ggcgcgcgat gctcaacatc tccgaactgc
     7321 tgccccagtt cgacgccagg tacccgacgt tgtcgccgcg cgaggtgatc gacttcatgg
     7381 tgcgcgatcc cgaaaacagc tcgtcgatct actgctgcct gcaggcggcg cgcgaaaacg
     7441 cgcgggccgt gcgcggttcg ctgacgaccg aggtctggga aacccacaac gacacctggc
     7501 taggcctgca gccgttgctg cgagagcgcc ggctcgagcg tgatccgcag gaattcttcg
     7561 agtgggtcaa gttccggtcg catctgtcgc gcggcgtcac gatcggcacg atgctcaagg
     7621 acgaggcgtt ctatttcatc cgtctcggta ccttcctcga gcgtgccgac aacactgcgc
     7681 gactgctgga tgtgaagtat cacggcgtag tccagcccgg cacgctgtac acgaacgccc
     7741 gtggcgaaga cgcgaactcc agcggcgaag acgcgaacga acagcatctg gatttctatc
     7801 actggacggc cgtgcttcga tcggtgtcgg cgttcgagat ctatcgcaag gtctatcgcg
     7861 acgtgatcac gcccgcacgc atcgccgcgt tgctgatcct gcgcggggac atgccgcgtt
     7921 cgttgctgtc gtgcgtcaac gaggtcagcc tcaatctgga gatggtccgc aacagccgct
     7981 cggaggaaac cgagcgccgc gccggactgc tgcgcgccga actgcgatac ggcaggatcg
     8041 aggacatcct ggcccgtggc atccacgagt tcctgacctc gttcctggag cgcgtcaacg
     8101 atctcggtgg ccgtatcagc caggacttcc tggtaccgct ggtggcgcac tgagaccgcg
     8161 gaggtgtcga caagcccatg cgctactgca tcgaacatct gaccgactat cgctacacgc
     8221 acgtggcggt ctcgacgcag cagacgctgc gcctgtcgcc gcgcatcgag gcgcaccagc
     8281 gggtcttcaa ctggaagatc cgcgtgcccg ggactctgtc gtccgcgaat gacgcgtatg
     8341 gcaacctgac ccacatgcac acgctgcaca gcaagcacga ccgtgtctgt atcgaggtcc
     8401 agggcgaggt cgaactcgat gcgctgaacg agggccggct ggccgagcgc ggtggtctgc
     8461 cgccgcaggt ctttgtcgcc gtgaccccgc tgaccgtagc ggatgagcgc attgctgact
     8521 tcgctcgtat ccagctgaag cgagcgactg cggccggcgt gctcgagttt gcgcaggcgg
     8581 tgcggtcggc catcgactac gaggccggaa cgacccatgt tcggacgacg gcggccgagg
     8641 cgctggcgct gggccgcggc gtctgccagg accacgcgca cgtgttcatc gcgggctgtc
     8701 gcgcggccgg catcccggcg cgttatgtct ccggctacta ctacagtgag cggcacgcgt
     8761 cccacgtcgc cagccatgcg tgggccgacg tctgggtgtc cgacggctgg acgtcggtcg
     8821 acatcacgca tgcgtgcttc gcgtccgacg cgttgtgccg gctcgccata ggtcgtgatt
     8881 acgacagtgc atctcccgtg cgcggcgtgc gtgccggcgg aggggaagag gccatgaaag
     8941 tcgcgctcac ggtgcggcgc gtggcgcaga cagcagacag gacgccggcc gcgcgtccgg
     9001 aagaggtgac ggcatgacgt attgcatcgg agtgcagctc gatgacgggc tcgtgttcct
     9061 gtccgactcg cgcaccaatg cgggggtgga tcacatctcc accttccgca agatgaccgt
     9121 tttcgagcat ccgggcgagc gcctgatggt gctgctgagc gcgggcaacc tggcagtgac
     9181 gcaagcggta cggcagatcg tctgcgaacg cgtcggtcct cacgacgaca gtgtctggaa
     9241 tgccaagaca ctcttcgagg ccgtgcgcgt cgtcggcgag gcgatacgcg aagtgcatca
     9301 gcgcgacggc caggcactca aggacgccgg catggagttc aattgcagct tcatattcgg
     9361 cggccagatc cgcggcgagc ggatgcgtct gtttcagctc tactccgcgg gcaactttat
     9421 cgaggccact ccggagaacg cgtacttcca gatcggcgag gccaagtacg gcaagccgat
     9481 catcgaccgt gtgatctcgc cgtcgacttc actcgaggag gcggccaagt gcgcgctgat
     9541 ttcaatggac tcgacgctgc gttccaatat ctcggtcggg ctgccgctgg acctgctggt
     9601 ctacgagcaa ggtgcgctgc gcgtcacgaa gttcgtgcag atcgaccatc acaaccagta
     9661 catgcagatg atccgcaac
//
LOCUS       DATIRT010000128         4983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1392933, whole genome shotgun sequence.
ACCESSION   DATIRT010000128 DATIRT010000000
VERSION     DATIRT010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392933"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4262)
                     /locus_tag="VLN25_03105"
     CDS             complement(<1..4262)
                     /locus_tag="VLN25_03105"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF016944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HSQ09594.1"
                     /translation="MKADIAPMTPSPEGSKAQRLEEVLALIAQRHASAERAMLTTFGL
                     EYFRQADQEDICERTAEDLYGMALSHLQLARQRTPGQPRLRVLNPTAHEAGFGSRHTI
                     VEVVNDDMPFLVDSTSVEVNRQGLTLHLILHPIFAVRRSESGELLELQPRAERPDWPR
                     ESFMHLEVDRLADPVARAGLAEGIERVLGDVRAAVVDWKPMVAQLRAVIRELDERPPP
                     VPREELTESRAFLQWLTEDHMLLLGYRCNDLQERDGELELVRVDGTGLGILREAGAPK
                     GISISFSTAPPNMRSLATSPSPMLLVTKATTRATVHRPGYVDYVGVKRYDGRGRVIGE
                     HRFVGLFTSAAYSTRVTEVPLVRGKVREVTERAGLAPASHLGKALTHILESYPRDELF
                     QISVDDLAEISNGILQLGERQRFRLFVRRDAFERFVSCLIFVPRENYTTELRRKFQWI
                     LLEAFNGSAAEFDVLLTDAALARIHITVRTTPGRIPPFDRRTVEAHLAVAARRWEDDL
                     REELSARLDEADAAQLLRHFGTAFPASYREHIDAASAVEDLQSLRRLARTELVLNLYR
                     PRDAAPHRLGFKVYRGSAPVPLSDSLPMLEHMGVRVLSERPYEITPDGAEPMWIHDFE
                     LEVPGSGEMDVAALAPLFEDAFERVFEGRVENDDFNRLVLKASVAADEIVVLRAYAKY
                     LRQIGFPLSQQYIETTLAAYPRVAGMLVRLFRLRFSLTERDDAAAGSQVRAIEQALDK
                     VPNASEDRVLLQYLALIQATMRTNFWRTGVGADGAAGARRTFLSFKFDPKKIPGLPEP
                     RPMFEIFVYSTRFEGVHLRGGKVARGGLRWSDRPEDFRTEVLGLVKAQMVKNTVIVPV
                     GSKGGFVLKKAPPSTEREAYLKEGIACYQDYLRGLLDLTDNLVQGQVVPPPHVVRHDS
                     DDTYLVVAADKGTASFSDYANAISAEYGHWLGDAFASGGSAGYDHKKMGITARGAWES
                     VQRHFREMGIDTQTTGFTCVGVGDMSGDVFGNGLLLSKHIRLLAAFDHRHIFIDPTPD
                     AATSFAERERLFALPRSSWADYNANLISAGGGIWSRAEKSIPVSPQAQQALGIAAAQL
                     TPTELIAAILKAPADLLYNGGIGTYVKSSWESHADVGDRANDALRVNGADLRCKVVGE
                     GGNLGLTQRGRVEAALAGVRLFTDAIDNSAGVDTSDHEVNIKILLGAAVAAGRLKLAE
                     RNALLAEMTDDVAHLVLEDNYFQTQALSVGSRTARKLLEQQQRFMRFLEKEGRLNRAI
                     EFLPDDETIAERRAAGLALTPPERAVLLAYAKMWLFDALLASDLPEDPWVASALTHYF
                     PATVRTRFAADIERHPLRREIIATQLLNDMVNRVGPSFVYQMTSGTGSTPAQVVRAYL
                     LTREVFDLRPTWKAIEALDNVVSDEMQAELLMELGRRTVRATT"
     gene            4447..>4983
                     /locus_tag="VLN25_03110"
     CDS             4447..>4983
                     /locus_tag="VLN25_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HSQ09595.1"
                     /translation="MFPAFAPRLAFVDVETTGSSPERERVTEVGLVGVDFDGDDLRVT
                     EWSSLINPGMPIPAEIQWLTGITNDMVRDAPAFADLAQELYDRLDGVVFVAHNARFDY
                     GFLRAEFARAGLNFHAKTLCTVRLSRHLYPDRSPHTLDAIIERFGLGGEQRHRALGDA
                     RVLWRLLQKLGDRHSPAEL"
BASE COUNT          823 a         1693 c         1613 g          854 t
ORIGIN      
        1 gtcgtcgcac gcacggtgcg tcgtcccagc tccatcagca gttcggcctg catctcgtcg
       61 ctgaccacgt tgtcgagcgc ctcgatcgcc ttccacgtcg gccgcaggtc aaagacctcg
      121 cgtgtcagca gataggcgcg cacgacctgc gcgggcgtcg aaccggtgcc cgaggtcatc
      181 tggtagacga agctgggccc gacgcggttg accatgtcgt tcagcaactg cgtggcgatg
      241 atctcccgcc gcagcgggtg gcgttcgatg tcggcggcga aacgcgtgcg cactgtcgcc
      301 gggaagtagt gcgtcagcgc gctcgccacc cacgggtctt ccggcagatc cgacgccagc
      361 agcgcatcga acagccacat ctttgcgtag gccagcagca cggcacgttc gggcggagtc
      421 agggcaagcc cggcagcgcg ccgctcggcg atggtctcgt catccggcag gaattcgatc
      481 gcgcgattga gccggccttc cttttccaga aagcgcatga agcgctgctg ctgctcgagc
      541 agcttgcgcg cggtgcgcga tccgaccgac agcgcctgcg tctggaagta gttgtcctcg
      601 agcacgagat gagcaacgtc gtcggtcatc tcggcgagca gcgcattgcg ctcggccagc
      661 ttgagcctgc ccgcagccac cgcagcgccc agcaggatct tgatattgac ttcatggtcc
      721 gaggtatcga ctccggccga gttgtcgatc gcgtccgtga aaagacgcac gccggcgagt
      781 gccgcctcga cgcgtccgcg ttgcgtcaga ccgagattgc cgccttcacc gacgaccttg
      841 cagcgcaggt cggccccgtt gacgcgcagc gcatcgttgg cccggtcgcc gacgtcggca
      901 tggctttccc aggacgactt gacgtaggtg ccgatgccac cgttgtagag cagatccgcc
      961 ggcgccttca ggatggcggc gatcagttcc gtcggagtca gctgcgccgc tgcgattccg
     1021 agcgcctgct gcgcctgcgg tgacacgggg atcgacttct ccgcgcgcga ccagatcccg
     1081 ccgcccgcgg aaatcagatt cgcgttgtag tcggcccacg aggagcgcgg cagcgcgaac
     1141 agccgttctc gctcggcaaa actcgtcgcc gcgtcgggcg tcggatcgat gaagatgtga
     1201 cgatgatcga acgcggccag cagccggatg tgcttcgaca gcagcaggcc gttgccgaac
     1261 acgtcgcccg acatgtcgcc gacaccgacg caggtgaacc cggtcgtctg agtgtcgatg
     1321 cccatctcgc ggaagtgacg ctggacgctt tcccacgccc cgcgcgccgt gatgcccatt
     1381 ttcttgtggt catagccggc gctgccgcca gaggcgaagg catcgccgag ccagtggcca
     1441 tactccgcgc tgatcgcatt ggcatagtcg gaaaagctcg ccgtgccttt gtcggccgcc
     1501 accacgaggt aggtgtcgtc gctgtcgtgt cgcacgacgt gaggtggcgg cacgacctgg
     1561 ccctgcacca ggttgtcggt caggtcgagc agtccgcgca ggtagtcctg gtagcaggca
     1621 atgccctcct tcaggtacgc ctcgcgctcg gtggatggcg gcgccttctt cagtacgaag
     1681 ccgcctttcg atccgacggg aacgatgacg gtgttcttga ccatctgcgc cttgaccagc
     1741 ccgagcacct cggtacggaa gtcctccggt cgatcggacc agcgcagtcc accgcgtgcg
     1801 accttgcctc cgcgcagatg tacgccctcg aaccgcgtcg agtacacgaa gatctcgaac
     1861 atcggccgcg gctccggcag gccggggatc ttcttcggat cgaacttgaa cgacaggaac
     1921 gtgcggcgcg ctccggccgc tccgtcggcg cccacgccgg tgcgccagaa gttcgtgcgc
     1981 atcgtggcct ggatcagcgc aaggtactgc agcagcacgc ggtcctcgct cgcattgggc
     2041 accttgtcca gcgcctgctc gattgcacgc acctggctgc ccgccgccgc gtcgtcgcgc
     2101 tcggtgaggc tgaagcgcaa acggaacagc cgaacgagca tgccggcgac gcgcggatag
     2161 gcggccagcg tcgtctcgat gtactgctgc gacagtggga agccgatctg ccgcaggtac
     2221 ttggcgtagg cgcgcagtac gacgatctcg tctgccgcca cgctggcctt gagcaccagc
     2281 cgattgaagt cgtcgttttc gacgcgccct tcgaacacgc gttcgaaggc gtcctcgaac
     2341 agcggcgcca acgccgcgac atccatctcg ccgctgcccg ggacctcgag ctcgaagtca
     2401 tggatccaca tcggctcggc gccgtcgggc gtgatctcgt acggtcgttc ggacagcacg
     2461 cgcacgccca tgtgttcgag catcggcagc gagtccgaca gcggcaccgg cgcgctgcca
     2521 cggtagacct tgaagcccag gcgatgtggc gctgcgtcgc gcggccggta caagttgagc
     2581 acgagctcgg tacgcgcaag gcgacgcagg ctctgcaggt cttcgaccgc gctggctgca
     2641 tcgatgtgct cgcggtacga cgccgggaac gccgttccga aatgtcgcag taactgcgcg
     2701 gcgtcggcct cgtccagccg ggcactcagc tcttcacgga ggtcgtcctc ccagcggcgt
     2761 gccgcgacgg ccaggtgcgc ctcaaccgta cgacgatcga acggcgggat gcgcccgggc
     2821 gtcgttcgta ccgtgatgtg aatgcgcgcg agcgcggcgt cggtcagcag cacgtcgaat
     2881 tcggccgccg aaccgttgaa cgcctcgagc aggatccact ggaacttgcg ccgcagttca
     2941 gtcgtgtagt tttcgcgcgg cacgaagatc aggcacgaga caaagcgctc gaacgcgtcc
     3001 cgtcgcacga agaggcgaaa acgctgacgc tcgccgagct gcagaatgcc gttcgagatc
     3061 tcggccaggt cgtcaaccga gatctggaac agctcgtcgc gcggatacga ctcgaggatg
     3121 tgcgtgagcg ccttgccaag gtggctggcc ggcgcaagcc cggcgcgctc cgtcacctct
     3181 cgcaccttgc cccgcaccag cggtacctcg gtcacgcgcg tactgtaggc ggcggaagtg
     3241 aagaggccga cgaagcgatg ctcgccgatg acccgacctc tgccgtcgta gcgtttgaca
     3301 ccgacgtaat cgacataccc gggccgatgc accgtcgcac gcgtcgtcgc cttcgtcacc
     3361 agcagcatcg gcgacggcga ggtcgccagg ctgcgcatgt tcggcggcgc cgtcgagaag
     3421 ctgatcgaaa tgcctttcgg tgccccggct tcgcgcagga tgccgagccc ggtcccgtcc
     3481 acgcgcacga gctccagctc gccgtcgcgc tcctgcagat cgttgcagcg atagccgagc
     3541 agcagcatat gatcctcggt cagccactgc agaaacgcac gcgactccgt cagttcctcg
     3601 cgcggaaccg gcggaggacg ctcgtcgagt tcgcggatga cggcgcgcaa ctgcgcgacc
     3661 atcggtttcc agtcgacgac ggcggcacgg acatcgccaa gcacgcgctc gatgccctca
     3721 gcgagtccgg cccgagcaac gggatcggcc aggcgatcca cctccaggtg catgaacgac
     3781 tcgcgcggcc agtcaggacg ctccgcgcgc ggctgaagct cgagcagttc gcctgactca
     3841 ctgcgtcgca ccgcaaagat cggatgcagg atcaggtgca gcgtcagccc ctggcgattg
     3901 acctcgaccg acgttgaatc gaccaggaac ggcatgtcgt cgttgacgac ctcgacgatg
     3961 gtgtggcgtg acccgaagcc cgcctcgtgt gccgtcggat tcaggaccct cagtcggggc
     4021 tgacccggcg tgcgctggcg cgccaactgc aggtgcgaaa gtgccattcc gtaaaggtct
     4081 tcggcagtcc tctcgcagat gtcctcctgg tctgcctggc ggaagtactc caagccgaac
     4141 gtcgtgagca tcgcccgctc ggcggaggca tgccgctgcg cgatcagtgc aagcacttcc
     4201 tcgagccgtt gcgccttgct cccttccgga ctcggcgtca taggggcgat atcggccttc
     4261 atcgtgtgtc tccgcgtgcc ggctgccggg caagtcccgg cgtagtccgc atttccagga
     4321 aacgcggtca gcccggcgtt gtcctgggag cgtgttcgag cctgccagta tgcccgcgcc
     4381 gcgcgggctt cgcgatcgtg cagtgccgga taatccggga cttcgcgcct ggccacccac
     4441 ctcggaatgt tccccgcctt cgctccacga cttgcgttcg tcgatgtcga gacgaccggc
     4501 tccagcccgg agcgcgagcg cgtgactgaa gtcggcctcg tcggcgtcga cttcgatggc
     4561 gacgacctgc gcgtcaccga gtggtcatcg ctgatcaatc cgggcatgcc gatcccggca
     4621 gagatccagt ggctgacggg catcaccaac gacatggtgc gcgacgcgcc cgcgttcgcc
     4681 gacctggcgc aggaactcta tgaccggctc gacggagtgg tgttcgtcgc gcacaacgcc
     4741 cgcttcgact acggcttcct gcgcgccgag ttcgctcgtg caggactcaa cttccatgcg
     4801 aagacgctgt gcacggtgcg actgtcgcgg cacctctacc ctgatcgatc gccgcatacc
     4861 ctggatgcca tcatcgagcg cttcgggctc ggcggcgaac agcgtcaccg cgccctcggc
     4921 gatgctcgcg tgctgtggcg gctgctgcag aagctcggtg accgtcactc gccggcagaa
     4981 ctc
//
LOCUS       DATIRT010000129         5664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_999715, whole genome shotgun sequence.
ACCESSION   DATIRT010000129 DATIRT010000000
VERSION     DATIRT010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5664
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999715"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VLN25_03115"
     CDS             complement(<1..182)
                     /locus_tag="VLN25_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09596.1"
                     /translation="MSELVFHPGLLLIAGAVILPWLRGSVRSAVVLLLPLAAIALVWS
                     IPDGVVWQVRFLGLTLT"
     gene            complement(175..435)
                     /locus_tag="VLN25_03120"
     CDS             complement(175..435)
                     /locus_tag="VLN25_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09597.1"
                     /translation="MNDRHWLDEPRNVRLLWRLFLVVLALTVLVEAFVDLHPHFAIER
                     LFGFHAWYGLLACAAMIGVAKALGVLLKRPDTYYDGELPRDE"
     gene            complement(440..1903)
                     /locus_tag="VLN25_03125"
     CDS             complement(440..1903)
                     /locus_tag="VLN25_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D family
                     protein"
                     /protein_id="HSQ09598.1"
                     /translation="MAATLIAVTLAVPVVGAVFIAAFHRHPNLREAATLLTAVLLFVC
                     VKALLPGVLGGAQPALHLLDVLPGLALAFKVEPLGMLFALVASGLWILNSVYSFGYMR
                     SNGESHQTRFYVCFALALASSIGIAYAGNVFTLFVFYELLTLITYPLVTHHGTADARR
                     GGRVYLGILIGTSMVLLLPAVIFTWQAAGTTDFQPGGILDGKLSAAALAGLLALYMFG
                     IGKAALMPFHRWLPAAMVAPTPVSALLHAVAVVKAGVFSVVKVLVYVFGVDVLAAASA
                     TDWLVVVAGFTILAASVVALRADNLKRRLAYSTVSQLSYVVLAAALLAPLSIVGAVMH
                     IAAHALGKITLFFAAGAIYTAAHKTEVSQLDGIGRRMPWTMGAFAIGALSMIGLPPAA
                     GFISKWYMLAGAMEAAQWLAVAVIIASTLLNAGYFLPIVYRAFFRPPAATGHDHPHGE
                     APLAMVLALTVTAAGTLLLFFVPDVPLALARQLLGKG"
     gene            complement(1903..3387)
                     /locus_tag="VLN25_03130"
     CDS             complement(1903..3387)
                     /locus_tag="VLN25_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D family
                     protein"
                     /protein_id="HSQ09599.1"
                     /translation="MSIAAHLPALQVVVPLLAAPLSVLLRDRRAAFAVALAASWAAFA
                     IAIALWLQVSASGTISYHIGSWAPPWGIEYRIDRLTSFVLCLVSGVAAVVLPYSWPSI
                     ERELPREQHYLYFTMFSLCLAGLLGITITGDAFNIFVFLEISSLSTYVLIALGRDRRA
                     LVAAYQYLIMGTIGATFIVIGIGLLYLQTGTLNLVDMGQRLQSISGHRSVLAALAFLT
                     VGLSLKLALFPLHQWLPNAYAYAPSAVSAFLAATATKVAVYALLRFYFSVFGATTVFR
                     QLPMAELMLVLALAGMFVASTVACFQNDLKRLFAYSSVAQIGYIILGLSFDSVTGLTA
                     TIVHLANHAVTKGGIFLLLGAVAASMGGVTLARTQGMGRVMPLTSFGIVLCGLSLIGV
                     PGTAGFISKWYLILAALEQGQWWLVFLIVLSSLLAVVYVWRFVESAYFKAPRADLADV
                     REAPASLLVPALLLVAATVYFGLDTSLTIGAAADAAAELLGATR"
     gene            complement(3384..3755)
                     /locus_tag="VLN25_03135"
     CDS             complement(3384..3755)
                     /locus_tag="VLN25_03135"
                     /inference="COORDINATES: protein motif:HMM:NF012636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HSQ09600.1"
                     /translation="MTTVSHFSYAVTIFLMIAGLFIVIARGNLIKKLVGLGIFQTSVY
                     LLYVAPGKLIGGTAPIVSDAFTVYSNPLPHVLILTAIVVGVATLALGLALVVRIREAY
                     SSIEEDEILDADLSDGVADRQ"
     gene            complement(3752..4177)
                     /locus_tag="VLN25_03140"
     CDS             complement(3752..4177)
                     /locus_tag="VLN25_03140"
                     /inference="COORDINATES: protein motif:HMM:NF009162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter subunit B"
                     /protein_id="HSQ09601.1"
                     /translation="MKRDLILRVVAKVFLPFILIFALYVQFHGDFGPGGGFQAGVIIA
                     AAVVFYALIYGLANARRVVPERLVEGMMAAGVLIYAGVGVIGLVLGGNYLDYFVLDRD
                     PVAGQHRGIFWVEVGVAVTVSGVMLKIFYMFADRGRIDE"
     gene            complement(4174..4716)
                     /locus_tag="VLN25_03145"
     CDS             complement(4174..4716)
                     /locus_tag="VLN25_03145"
                     /inference="COORDINATES: protein motif:HMM:NF009159.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HSQ09602.1"
                     /translation="METLIINVLLVLMAVAVVVLARVRNLFSVIVLSGLYSFLMATVL
                     VALDAVDVAMTEASVGAGISTVLLLGALHLCKSEEAKPVQKPWLPLLVSVACGLLLVY
                     GSLGLPAFSDPAAPIHTHVAPRYLQEGPKETGVPNVVTAVLASYRGYDTLGETTVVFT
                     AGAGVVVLLRRRRGRKEQQK"
     gene            complement(4704..5033)
                     /gene="mnhG"
                     /locus_tag="VLN25_03150"
     CDS             complement(4704..5033)
                     /gene="mnhG"
                     /locus_tag="VLN25_03150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HSQ09603.1"
                     /translation="MSALIDGLSWVSLIAGGVFCVIGAVGMLRMPDFYTRLHAASVIE
                     TLGGGLILLGLLLQAGFTLVAAKLLIVALLVFFASPTATHALAKAAMERGVKPLLYRP
                     EGTPSKR"
     gene            complement(5030..5314)
                     /locus_tag="VLN25_03155"
     CDS             complement(5030..5314)
                     /locus_tag="VLN25_03155"
                     /inference="COORDINATES: protein motif:HMM:NF015993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HSQ09604.1"
                     /translation="MFAAASLAMLVTLALVLVRAALGPTVFDRAQAANTVGTCALILL
                     ALFGFLTGRPEFLDLAIVYGLLNVIGTIAVLKYFRYGGLGDTGDEREERP"
     gene            complement(5314..>5664)
                     /locus_tag="VLN25_03160"
     CDS             complement(5314..>5664)
                     /locus_tag="VLN25_03160"
                     /inference="COORDINATES: protein motif:HMM:NF014012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HSQ09605.1"
                     /translation="RMDVIDAEGHPVQLGWRIVAYWAWLSKEIVKSAWDVSRIIVNPR
                     LPISPTLVSFKPSQKTTVGLVIHANSITLTPGTITIDAGKDEFLVHSLTRAGAEGATT
                     GDMDRRVRACEGVQ"
BASE COUNT         1216 a         1853 c         1809 g          786 t
ORIGIN      
        1 gtcagcgtga ggccgaggaa gcgcacctgc cagacgacgc cgtcaggtat cgaccagacc
       61 agggcgattg cggccagcgg cagcagcagc accacggcag agcgcaccga cccgcgcagc
      121 caaggcagga tcaccgcgcc cgcgatcagc aggagtccgg gatggaacac cagttcactc
      181 atcgcgcgga agctcgccgt cgtagtacgt atcgggccgt ttgagcagca cgccgagcgc
      241 cttcgcgacg ccgatcatcg cggcgcaggc cagcaggcca taccaggcgt ggaagccgaa
      301 caggcgttct atcgcgaaat gcggatgcag gtcgacgaac gcttcgacca gcaccgtcag
      361 cgcgaggacg acgagaaaca ggcgccacag cagtcgcacg ttgcgcggtt catccagcca
      421 atggcggtcg ttcatcgggt caccctttac cgagcaactg ccgcgcgagc gcaagcggta
      481 cgtccggcac gaaaaacagc agcagcgtgc cggcggcggt gacggtcagc gcaagcacca
      541 tcgccagcgg ggcctcgcca tgcggatggt cgtgcccggt cgccgccggc gggcggaaga
      601 aggcacggta gacgatcggc aggaagtagc ccgcgttgag cagcgtactg gcgatgatca
      661 cggccacggc cagccattgc gcagcctcca tcgcaccggc cagcatgtac cacttgctga
      721 tgaagcccgc cgcgggcggc aggccgatca tcgacagcgc accgatcgcg aacgctccca
      781 tcgtccaggg catgcgccgt ccgatgccgt cgagctggct gacttcggtc ttgtgggccg
      841 cggtgtagat tgcgccggcg gcgaagaaca gcgtgatctt tcccagcgca tgggcggcga
      901 tgtgcatcac cgctccgacg atcgatagcg gcgccagcag cgcggccgcc agcaccacat
      961 acgacagctg gctcacggtg gagtaggcaa gccggcgctt gaggttgtcg gcccgcagcg
     1021 cgacgaccga cgccgcaagg atggtgaacc ccgcgacgac cacaagccag tccgtcgctg
     1081 aagccgcagc caggacatcg acgccgaaga catagaccag gaccttgacc acgctgaaca
     1141 cgccggcctt gacgaccgcc accgcgtgca acagtgccga cacgggggtc ggtgccacca
     1201 tggctgcggg cagccaccgg tgaaacggca tcaacgccgc cttgccgatg ccgaacatgt
     1261 acagcgccag cagtccggcc agtgcggcag ccgaaagctt gccgtccaga attccgcccg
     1321 gctggaagtc ggtcgtgccc gctgcctgcc aggtgaagat caccgccggg agcagcagca
     1381 ccatcgacgt gccgatcaag atccccaggt agacgcgccc gccgcgccgc gcgtccgccg
     1441 tgccatggtg ggtcaccaag ggataggtga tcagcgtgag aagttcgtag aagacaaaga
     1501 gcgtgaagac gttgccggca taggcaatgc cgatcgacga cgccaacgcc agggcgaagc
     1561 agacatagaa gcgggtctga tgggactcgc cattgctgcg catgtacccg aacgagtaga
     1621 ccgagttcag gatccacagg cccgaggcga cgagcgcaaa cagcatgccc agcggctcga
     1681 ccttgaatgc gagtgccaat ccgggcagga cgtccagcag gtgcaaagcc ggctgcgccc
     1741 ctccgagaac gcccggcagc agcgctttca cgcacacgaa cagcagcacg gccgtcagca
     1801 gcgttgcggc ttcccgcaga ttgggatgtc gatgaaaggc ggcgatgaac acggcgccga
     1861 ccaccgggac ggccagcgtg accgcgatca gcgtcgcggc catcaccgtg tcgctcccag
     1921 caattcggcg gctgcatcag ccgctgcgcc gatggtcaac gaggtgtcga ggccgaagta
     1981 aacggttgcg gcgaccagca gcagcgcggg aaccagcagc gatgccggag cctctcgcac
     2041 atccgccagg tccgccctcg gcgccttgaa gtaggccgac tcgacgaagc gccagacgta
     2101 caccacggca agcaaagacg acaacacgat caggaacacc agccaccact ggccctgctc
     2161 cagggcggcc aggatcaggt accacttgct gatgaaccct gccgtgcccg gcacaccgat
     2221 gagcgacagg ccgcacagca cgatgccgaa actggtcagc ggcattacac ggcccatgcc
     2281 ttgcgttcgc gcaagcgtga cgccgcccat cgaggcagcg actgcgccga gcagcaggaa
     2341 gatgccgccc ttggtcaccg catggttggc aagatgcacg atcgttgccg tcaggccggt
     2401 caccgagtcg aacgacaggc cgaggatgat gtatccgatc tgtgcaacgc ttgagtaggc
     2461 gaacaggcgc ttaaggtcgt tctggaagca cgccacggtc gaggcaacaa acatgcccgc
     2521 aagcgccagg accagcatca gttcggccat cggcaactgc cggaacacgg tggttgcacc
     2581 gaacacggaa aaatagaaac ggagcaacgc atacaccgcc accttggtgg cggtcgccgc
     2641 caggaacgcg gaaaccgccg acggtgcgta cgcgtacgcg tttggcagcc actggtgcag
     2701 tgggaagagc gcgagcttca gcgacagccc gacagtcagg aacgccagtg ccgccaggac
     2761 gctgcggtgt ccggaaatcg actgcaggcg ctgccccatg tcgaccaggt tgagggtacc
     2821 ggtctgcagg tagagcaggc cgatgccgat cacgatgaaa gtcgccccga tcgttcccat
     2881 gatgaggtac tggtacgcgg cgaccagtgc ccggcgatcc ctccccagcg cgatcagtac
     2941 gtaggtcgac agcgaggaga tctcgaggaa cacgaagatg ttgaacgcgt cgccggtgat
     3001 cgtgatgccg agcagccccg ccaggcacag cgagaacatc gtgaagtaga ggtagtgctg
     3061 ttcgcgcggc agttcacgct cgatgctcgg ccagctgtag ggcagcacca ccgcggcgac
     3121 accggagacc aggcacagca caaacgaggt caggcggtcg atccggtact cgatgcccca
     3181 cggaggcgcc cagctgccga tgtggtacga gatagtgccg gatgcggata cctgcagcca
     3241 cagcgcgatg gcaatcgcaa acgcggccca ggaagcagcc aacgcgacgg caaacgccgc
     3301 ccgccgatcg cgcagcagca cactcagtgg agcagcgagc agcgggacga caacctgcag
     3361 cgcgggcagg tgcgcggcga tgctcattgc cggtcggcga ctccatcgct gaggtcggca
     3421 tcgagaatct cgtcctcctc gatgctgctg taggcctccc ggatccgcac gaccagtgcc
     3481 agaccgagcg ccagtgtcgc gacgccgacg acgatggccg tcaggatgag cacgtgcggc
     3541 agtggattgg aatacaccgt aaatgcatcg ctgacgatgg gcgccgtgcc accgatcagc
     3601 ttgcccggtg cgacgtaaag cagatacacc gacgtctgga aaattcccag gccaacgagc
     3661 ttcttgatca ggttgccgcg ggcaatgacg atgaaaagcc cagcgatcat caggaaaatc
     3721 gtcaccgcat agctgaagtg gctgacggtg gtcactcgtc aatccttccg cggtcggcga
     3781 acatgtagaa gattttcagc atcacgccgg acacggtgac cgcgacaccg acctcgaccc
     3841 agaagatgcc gcggtgctgg ccggcgaccg ggtcccggtc gagcacgaag taatcgaggt
     3901 agttgccgcc aagcacaagt ccgattacgc caaccccggc atagatcagc accccggcgg
     3961 ccatcatgcc ttccaccagg cgctccggca ccacgcggcg tgcattcgcc aggccgtaga
     4021 tcagtgcata gaaaacgacg gcagccgcaa tgatcacacc cgcctggaag cctccgccgg
     4081 gaccgaagtc gccgtggaac tgcacataca aggcaaagat gaggatgaac ggcaggaaca
     4141 ccttggcgac gacccgtagg atcagatcgc gcttcacttc tgctgctcct tgcgcccgcg
     4201 ccggcgacgc aggagtacca cgacgccagc ccctgcggtg aagaccaccg tcgtttcacc
     4261 gagcgtgtca taaccgcggt agctcgccag cactgccgtc acgacattcg gaacgccggt
     4321 ctccttcggc ccctcctgca ggtagcgtgg tgcgacgtgg gtgtggatcg gcgctgccgg
     4381 atcggaaaag gccggcagtc cgagactgcc gtagacgagc agcagtccgc atgccaccga
     4441 gaccagtagc ggaagccagg gcttttgaac cggcttcgcc tcttcgctct tgcacaggtg
     4501 cagcgcgccg agcagcagga ccgtcgatat gccagcgccg accgaggcct cggtcatcgc
     4561 gacatcgacg gcatcgagcg ccaccaggac ggtcgccatc aaaaagctgt atagcccgga
     4621 cagcacgatg accgaaaaca gattgcggac gcgggccagc acgaccaccg cgaccgccat
     4681 caacaccagc agaacattga tgatcagcgt ttcgatggtg tcccctcggg acgatagagg
     4741 agtggcttca cgccgcgctc catcgcggcc ttcgccaggg cgtgcgtcgc tgtcggacta
     4801 gcgaagaaca cgagcagggc aacgatgagc agtttggcgg ccaccaacgt gaatccggcc
     4861 tgcaggagca ggccaagcag gatgagtccg ccgccgaggg tctcgatcac gctcgctgca
     4921 tgcagccgag tgtaaaagtc cggcatgcgc agcatcccaa ccgccccgat cacgcagaac
     4981 acaccgccgg cgatcaggga cacccagctc agtccgtcga tcagcgcgct catggtcgtt
     5041 cctcgcgctc gtcgcccgta tcgccgaggc cgccgtagcg aaagtacttg agtaccgcga
     5101 tcgtgccgat gacgttgagc aggccgtaca cgatcgcgag atcgaggaac tcgggccgcc
     5161 ccgtgagaaa gccgaacagc gccagcagga tcagggcaca ggtgccgacc gtgttggccg
     5221 cctgtgcacg gtcgaagacc gttgggccga gtgctgcgcg caccagcacc agagccagcg
     5281 tcacgagcat ggcaaggctg gccgcggcga acatcactga acgccctcgc aggcacggac
     5341 gcggcgatcc atgtcgccgg tcgtggcgcc ctccgcgccg gcacgggtca gggaatgaac
     5401 caggaattcg tctttcccgg cgtcgatcgt gatcgtccca ggcgtgagcg tgatcgagtt
     5461 tgcgtgaatg acaagaccga cggtggtctt ctggctcggc ttgaacgaca ccagggtcgg
     5521 gctgatcggc aaccgtggat tgacgatgat ccggctgaca tcccacgccg actttacgat
     5581 ttccttcgac agccatgccc agtaggcgac gatgcgccaa ccgagctgca ccggatgccc
     5641 ttccgcatcg atcacatcca tgcg
//
LOCUS       DATIRT010000130         6681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_999748, whole genome shotgun sequence.
ACCESSION   DATIRT010000130 DATIRT010000000
VERSION     DATIRT010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6681
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999748"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VLN25_03165"
     CDS             <1..762
                     /locus_tag="VLN25_03165"
                     /inference="COORDINATES: protein motif:HMM:NF022646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 family protein"
                     /protein_id="HSQ09606.1"
                     /translation="QARFTPDALAGELATLRQPHRQTFERPYGWGWLLKLAAELEALA
                     QEQSGTSAWRDAMRPLASEFANRFIAYLPRADYPSRGGAHGNTAFALLLALAWCDTVQ
                     HRALRQAIAERANRWFGRDRRYPAAYEPGGEDFLSAGLVEAALMLRVVDGCSFADWWA
                     LFRPLDDALAAWLAPVTVSDPADAKIVHLHGVNLSRAWCWSLLSPELNPELREPVDRA
                     IAAHLEISLPAATRGDYVGTHWLASFALLALDDAR"
     gene            822..2372
                     /locus_tag="VLN25_03170"
     CDS             822..2372
                     /locus_tag="VLN25_03170"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSQ09607.1"
                     /translation="MSQWDVIVIGSGIGGLSAAAALAREGKRVLVVERHSQLGGLTQS
                     FERNGYRFNVGVHYIGGAGDIEGRAGPAKKIFDVLTPHGIPMASMGPVYDRVHFPGMT
                     IEFEHPAARLVETLKVRFPTEAAGIDRYFDSMRAAGKALEAVFAAHSMPQIVARGLMW
                     WKEDDVERWVGRTLWEVIQECVGDPKLQAVLAAQWGDHGGRPTQASFALHAVVMSSYF
                     DGAWYPVGGSGVFAEALGQAVRAGGGELRASEPVAAIRVSEGRVQGVTLASGAMIDAP
                     CVISDAGVRTTLRLLPSEEVDYEWARDVLQIEPSVGYVGLYLGLDGDIAANGASTAND
                     WIYESWDVDQLWRNPLIEPDAPMMFVSFPSLKDPLHDPGKSLRHTCEIVAFVDPDAFA
                     PWERESMKRGDTRDPGYLELKEQIEQNLLSQFGRHFPRLAPMVRYVTSSTPISVSTYT
                     GAEHGAMYGLATTPARFLSDALRPRTPIGGLFLAGQDACCPGVTGALLGGLMAAVSVE
                     PRLLTLLR"
     gene            2466..2882
                     /locus_tag="VLN25_03175"
                     /pseudo
     CDS             2466..2882
                     /locus_tag="VLN25_03175"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
     gene            complement(2804..2974)
                     /locus_tag="VLN25_03180"
     CDS             complement(2804..2974)
                     /locus_tag="VLN25_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09608.1"
                     /translation="MRTWVVLTKTFIGAWETRSKSTLSARMSRSGLLSRGLRSYGENT
                     RAARSIATKIGE"
     gene            3017..3523
                     /locus_tag="VLN25_03185"
     CDS             3017..3523
                     /locus_tag="VLN25_03185"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HSQ09609.1"
                     /translation="MLLASACLPTIFQAVEIDGEAYWDGGYTGNPTITPLVRECKSED
                     TILVQINPVERPGTPSTDNEIQNRLNEVSFNATLLKEMRAAALLRRVAKTQTGELGML
                     ARMRFHRITSPMMIELGYSSKMLAEWAFFQMLREEGRRVAEEFLANHGHDLGINATLD
                     LNEMLDFE"
     gene            complement(3602..4654)
                     /locus_tag="VLN25_03190"
     CDS             complement(3602..4654)
                     /locus_tag="VLN25_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HSQ09610.1"
                     /translation="MAAVPNPELHWESEPGSDEIIVKAEGIAKYFDVSPPWLNRVLER
                     SGRQILHAVDGISFTIRRGETLSLVGESGCGKSTVARLLVGLYTPTRGRVEFFGHDLA
                     EMNTASGRSLRQKLQMIFQDPFASLNPRWRVRDIIAEPLIAHGLMRDKVQIAARVDEL
                     LHQVKLGPADKEKYPHQFSGGQRQRLSIARALATKPEFLVCDEPTSALDVSVQAQVLN
                     LMKDLQRELGLTYLFISHNLAVVHHISDRVGVMYLGKIVELAPRRVLFDAPRHPYTRM
                     LLDAIPDLEMTGRARTPVAGEVPNPLNPPSGCTFHPRCPHANARCKAEVPVLRIERHG
                     AGETEVACHAVEEGRI"
     gene            complement(4659..5633)
                     /locus_tag="VLN25_03195"
     CDS             complement(4659..5633)
                     /locus_tag="VLN25_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSQ09611.1"
                     /translation="MLEVRNLRIEFPTRRGTLLAVDDVSFSIAPGEVLGVVGESGAGK
                     SLTGAAIIGLLEPPGRIAAGEIKLAGLRIDNLPYDDMRKIRGREIGAIFQDPLTSLNP
                     LYTVGQQIVETITTHLNVSSSQARERAVELLVSTGIPAAERRIDHYPHQFSGGMRQRV
                     VIALALAAQPKLVIADEPTTALDVSIQAQIISLLKQLCRDYGTAVMLVTHDMGVIAET
                     ADRVAVMYAGRIAEIGPVSDVIHRPQHPYTVGLMGSIPSMIADKERLTQIDGAMPRLT
                     AIPDGCAFHPRCTQAFERCSRERPDLLPAGASFASCWLHDPASLRRAA"
     gene            complement(5651..6043)
                     /locus_tag="VLN25_03200"
     CDS             complement(5651..6043)
                     /locus_tag="VLN25_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09612.1"
                     /translation="MKTRFVSAALLITGAGLLSGCATSFHGSYLVGDRYFKANMDTQP
                     VVILGVDNWDTVQRRTLVEPGVRVVRVQAMPVPGAPQETASFKLDVKPCHTYYIVAVR
                     ENPIAVSFTPTVDYAQPLAGCTPLPEKK"
     gene            complement(6090..>6681)
                     /locus_tag="VLN25_03205"
     CDS             complement(6090..>6681)
                     /locus_tag="VLN25_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSQ09613.1"
                     /translation="MGVGLGLLSGYVGGRIDAFIMRVCDVMLSFPAILVALLIDGVAR
                     AVLPRDSHNELAFGVLIIAIALSGWVQYARTVRGSTLVEKNKEYIQAARVIGVSPFKI
                     MFKHVLPNVLGPVLVLATVHIATAIITEATLSFLGVGVPPTTPSLGTLIRIGNEFLFS
                     GEWWITIFPGLALILLVLSVNLLGDWLRDALNPKLR"
BASE COUNT         1170 a         2185 c         2172 g         1154 t
ORIGIN      
        1 caggcccgct ttacgccgga tgccctcgcc ggtgaactcg ccacgctgcg gcagccccac
       61 cgtcaaacct tcgagcggcc ctatggctgg ggctggctgc tgaagctggc cgccgaactg
      121 gaagccctgg cccaggagca gagtggcacc tctgcctggc gcgatgccat gcggcctctc
      181 gccagcgagt tcgccaatcg attcatcgcc tatctgccgc gcgccgacta cccgtcgcgg
      241 ggcggtgcac acggcaacac ggccttcgcc ctgctgcttg cactcgcctg gtgcgacacg
      301 gtccagcacc gtgccctgcg tcaggcgatc gctgagcgcg ccaatcgctg gttcggccgc
      361 gaccgccgct atcctgccgc atacgagccc ggtggcgagg actttctctc ggcaggtctg
      421 gtcgaggccg cgttgatgct gcgcgtcgtc gacggctgtt cgtttgccga ctggtgggcg
      481 ctcttccgcc cgctggacga tgccctggcc gcctggttgg cacccgtgac tgtcagcgat
      541 ccggccgacg ccaagatcgt gcacctgcac ggagtgaacc tctcgcgcgc atggtgctgg
      601 tccctgctgt ccccggagct gaatccggag ctgcgcgaac ctgtcgatcg cgccatcgcc
      661 gcacatctgg agatatcgct gccggccgcc acccgcggcg actatgtcgg tacgcattgg
      721 ctcgcgtcat tcgcactgct cgctctggac gacgcgcgct gaccgcggtc aacaccgctg
      781 gcgatcgggt aaggtgagat acgcgattcg ggaggtgcat cgtgtctcag tgggacgtca
      841 tcgtcatcgg ttccggcatc ggcggactgt ccgcggcggc agcgctcgcg cgtgagggca
      901 agcgtgtcct cgtcgtcgag cgccacagcc agctcggtgg cctgacccag tccttcgaac
      961 gcaacggcta ccgtttcaac gtcggcgtgc attacatcgg tggtgcgggc gacattgagg
     1021 gccgcgccgg gccggcaaag aagatcttcg acgtcctgac gcctcatgga atcccgatgg
     1081 cgtcgatggg gccggtctac gaccgtgtgc actttcccgg gatgacgatc gagttcgaac
     1141 acccggccgc gcgcctggtg gagacgctga aggtccgctt tcctaccgaa gctgcgggca
     1201 tcgaccgtta cttcgacagc atgcgtgccg ccggcaaggc gctcgaagcc gtgtttgcgg
     1261 cgcattcgat gccgcagatc gtcgcgcgcg gactgatgtg gtggaaggaa gacgacgtcg
     1321 agcgctgggt cggccgcacg ctgtgggaag tcatccagga gtgcgtcggt gatcccaagc
     1381 tgcaggccgt gctggccgca cagtggggcg accatggcgg acgacccacc caggccagct
     1441 tcgcgttgca tgcggtggtg atgtcgagct acttcgacgg cgcctggtac ccggtgggcg
     1501 ggtctggcgt cttcgccgaa gccctcgggc aagccgtgcg ggccggcggt ggcgaactgc
     1561 gggcgagtga accggtggcc gcgattcgcg tgagcgaggg ccgagtgcag ggtgtgacgc
     1621 tcgcgagcgg cgccatgatc gacgcgccgt gcgttatctc ggatgccggc gtacgcacga
     1681 cactgcgcct gctgccgtcg gaggaagtgg attacgagtg ggcccgcgat gtactgcaga
     1741 tcgaaccgtc ggtcggctac gtcggcctgt acctcgggct cgacggcgac atcgctgcca
     1801 atggtgcgag cacggccaat gactggatct acgaaagctg ggatgtcgac cagctctggc
     1861 gcaacccgct gatcgagccc gacgcaccga tgatgttcgt ctcgttcccc tctttgaagg
     1921 atccgctcca cgatccgggg aaatccctgc gccatacctg cgagatcgtt gcctttgtcg
     1981 atcccgacgc cttcgccccc tgggaacgcg aaagcatgaa gcgtggcgac acgcgcgatc
     2041 caggctatct cgaactcaag gaacagatcg aacagaacct gctgagccag ttcgggcgtc
     2101 acttcccgcg actggcgccg atggttcgct acgtcacgtc atcgacgcca atctcggtgt
     2161 cgacctatac cggtgccgag cacggcgcga tgtacgggct ggcgaccacg ccggcccggt
     2221 tcctgagcga tgcactgcgt ccgcgcacgc cgattggcgg gctgttcctg gccggccagg
     2281 acgcatgttg ccccggtgtg acaggcgctc tgctcggtgg cctgatggcc gccgtgtcgg
     2341 tcgagccgcg gctgctcacg ctgttgcgct gacctcgctc ggaccggatg cggccgtccg
     2401 agccgaatct acgtttgtgt tccaatcgaa accactccgg tactgcatag atcgatcacg
     2461 caaccatgaa aaagaaatcc acaaagactt cctcgaagaa atccgcgccg tgcaaaacgg
     2521 ttctcgtcga cctggccctg caggggggcg gctcgcacgg agcgtttacc tggggcgtac
     2581 tcgatcgcct gctcgaggaa tcgtggctgc agttcgaggg ggtgtccggc acatcggcgg
     2641 gcgcgatgaa cgcggtcgtg atggccgacg gcatggctgc cggagggcac gcacaggcgc
     2701 gtatcgcgct cgacaacttc tggcgccgtg tttcggacgc ggccatcatg agtccgttcc
     2761 gccgcgggcc gcttgaagtc ctgaccggcc gatggacgct cgattactcg ccgatcttcg
     2821 tcgcgatgga tcttgcggcg cgcgtgttct cgccgtacga cctcaatccg cgcgacaaca
     2881 acccgctgcg cgacatcctc gccgaaagcg tcgacttcga gcgcgtctcc caggcgccga
     2941 tgaaggtctt cgtcaacacc acccacgtgc gcaccggccg cagccgcgtg ttccgcaacc
     3001 gggaactgac cctgacgtgc tgctcgcgtc ggcctgcctg ccgacgattt ttcaggcggt
     3061 cgagatcgac ggcgaggctt actgggacgg cggctacacg ggtaatccga cgatcacgcc
     3121 gctggtgcgt gagtgcaagt ccgaagacac gatcctcgtg cagatcaatc cggtcgaacg
     3181 ccccggaacg ccgagcacag acaacgagat ccagaaccgg ctgaacgagg tctcgttcaa
     3241 tgccactttg ctgaaggaaa tgcgcgccgc ggcgttgctg cgccgggtgg ccaagacgca
     3301 gaccggcgaa cttggcatgc tggcgcggat gcgttttcat cgcatcacca gtccgatgat
     3361 gatcgagctc ggctattcgt caaagatgct ggcggagtgg gccttcttcc agatgctgcg
     3421 tgaggagggg cgtcgagtgg ccgaagagtt ccttgcgaac cacggccatg atctcggcat
     3481 caatgcgaca ctcgacctca acgaaatgct cgacttcgag taagcaggac cactgacgtc
     3541 gggcaacgca ggcgttgcgt cgggaccgtc ccgagccgcc gccgatgccg cgcgcgagcc
     3601 gctagattcg cccctcttcc acggcgtggc aggccacctc tgtctctccg gcaccatggc
     3661 gttcgatgcg cagtaccggc acctcggcct tgcaccgtgc attggcatgc ggacagcgcg
     3721 ggtggaaggt acacccgctt ggcgggttca gtggattggg gacttcccca gccactggcg
     3781 tgcgcgcgcg gccggtcatc tcgagatccg ggatcgcgtc gagcagcatg cgcgtatacg
     3841 ggtggcgtgg tgcgtcgaac agcacgcgcc gcggcgcgag ttcaacgatc ttgcccaggt
     3901 acatcacgcc gacgcggtcc gaaatgtgat ggaccaccgc caggttgtgc gagatgaaca
     3961 gatacgtgag ccccagttcg cgctgcaggt ccttcatcag gttgagcacc tgcgcctgca
     4021 ccgagacatc gagcgctgac gtcggctcgt cgcatacgag gaattccggc ttggtcgcca
     4081 gcgcacgtgc gatcgacagc cgctgccgct gcccacctga aaactggtgc ggatacttct
     4141 ccttgtcggc cggtccgagc ttcacctgat gcagcaactc atcgacccgt gcggcgatct
     4201 gtaccttgtc gcgcatcagt ccatgcgcga tcagcggctc ggcgatgatg tcgcgcaccc
     4261 gccagcgcgg attcaggctc gcaaacgggt cctggaagat catctgcagc ttctggcgca
     4321 ggctgcggcc cgaggccgtg ttcatctcgg ccagatcgtg cccgaagaac tcgacacgac
     4381 cgcgcgtcgg cgtgtagagg ccgaccagca agcgcgccac agtggacttg ccgcaccccg
     4441 attcgccgac cagcgagagc gtctcgccgc gacggatggt gaagctgatg ccgtcgaccg
     4501 catgcagtat ctggcggccg ctgcgctcga gcacgcgatt cagccacggc ggcgatacat
     4561 cgaagtactt ggcgattccc tcggccttga cgatgatctc gtcggaaccc ggttccgact
     4621 cccagtgcag ttccgggttg gggacggcgg ccatcgcgtc aggccgcacg acgcagcgac
     4681 gcagggtcat gcagccagca cgacgcaaac gacgcaccgg ctggaagcag atcaggccgc
     4741 tcacgcgagc atcgctcgaa tgcctgggtg caacgcggat ggaatgcgca cccgtcggga
     4801 atcgccgtca ggcgcggcat cgcgccatcg atctgcgtga gccgctcctt gtccgcgatc
     4861 atgctgggaa tcgagcccat cagtcccacc gtatagggat gctgcggacg atggatgaca
     4921 tccgacaccg gcccgatctc ggcgatgcgg ccggcataca tgacggcaac ccggtcggcc
     4981 gtctcggcga tcacgcccat gtcgtgcgtg accagcatca ccgcggtgcc gtagtcgcgg
     5041 cacaactgct tcagcaggga aatgatctgg gcctggatcg aaacgtcgag ggcggtcgtc
     5101 ggctcgtcgg cgatgaccag cttgggttgc gctgcgagcg ccagtgcgat gaccacgcgt
     5161 tgccgcatcc cacccgagaa ctggtgcggg taatgatcga tgcgccgctc tgccgccggg
     5221 atgccggtgg acaccagcag ttcgaccgcg cgctcgcgcg cctggctcga cgagacgttc
     5281 aggtgcgtgg tgatcgtttc gacgatctgc tggccgaccg tgtacagcgg attcaatgac
     5341 gtcaacgggt cctggaagat cgcgccgatc tcgcgcccac ggatcttgcg catgtcgtcg
     5401 tacggcaggt tgtcaatgcg caggcccgcc agcttgatct cgccggccgc aatgcggccg
     5461 ggaggctcaa gcagcccgat gatcgctgcg ccagtcagcg acttgccggc gccggattcg
     5521 ccaaccacgc cgagcacttc gcccggtgcg atcgagaacg acacgtcatc gacggcaagc
     5581 agcgtgccgc gccgcgtcgg aaattcgatg cggagattgc ggacttcgag catcggactc
     5641 gccatgcgag ctatttcttc tctggcagcg gcgtgcagcc ggccagcggc tgcgcgtaat
     5701 ccaccgtcgg agtgaagctg accgcgatcg ggttctcgcg caccgcgacg atgtagtagg
     5761 tgtggcaagg cttgacgtcg agcttgaatg acgcggtctc ttgcggggcg cctggcaccg
     5821 gcatggcctg gacgcggacc acgcggacac cgggctcgac cagcgtgcgc cgctgcaccg
     5881 tgtcccagtt gtcgaccccc aggatgacca ccggctgggt gtccatgttg gccttgaagt
     5941 agcgatcccc caccaggtac gacccgtgaa acgaagtagc gcatcccgag aggaggcccg
     6001 caccggtgat cagcaaagca gcagagacga atcttgtctt catgaagttt ctcctttgga
     6061 gaaggtacta cgcaggcaac accgccagct caacgcaact tcggattcaa cgcatcgcgc
     6121 agccagtcac ccagcagatt gaccgacagc acgagcagga tcagggcaag gccggggaaa
     6181 atggtgatcc accactcccc ggagaacagg aattcgttgc cgatccggat cagcgtgccc
     6241 agcgagggcg tggtcggcgg cacgccgaca cccaggaagg acagcgtcgc ctcggtgatg
     6301 atcgcagtcg cgatgtgcac ggtagcgagc acgagcaccg gccccagcac gttcgggagc
     6361 acatgcttga acatgatctt gaacggcgag accccgatga cccgggccgc ctggatgtac
     6421 tccttgttct tctccaccag cgtggagcct cgtaccgtgc gtgcgtactg cacccaaccg
     6481 gagagtgcga tcgcaatgat caacacgccg aacgccagct cgttgtgcga atcgcgcggg
     6541 agcacggccc gcgccacacc gtcgatcagc agcgctacca ggatggccgg gaacgacagc
     6601 atgacgtcgc acacacgcat gatgaaagcg tcgatgcggc cgcccacata gcccgacagc
     6661 aaaccgaggc cgacgcccat c
//
LOCUS       DATIRT010000131         2297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1294499, whole genome shotgun sequence.
ACCESSION   DATIRT010000131 DATIRT010000000
VERSION     DATIRT010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294499"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VLN25_03210"
     CDS             <1..688
                     /locus_tag="VLN25_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09614.1"
                     /translation="QTVFWGLTVNLWLLQFSLTGCHGGFLMTATDGAFAQWLSEAQLA
                     EDRLTAEQRGLLRAAFAFRQCCGQDYYSTRLLSHFLLHCDANLKVAQIARLVGISRPT
                     ASHQQGVSSKEAIQAAHHRMAGRPHGKLLPRYAGPIAEYILSHVDATRYDILDFIQRT
                     WNVRVSTVALHHFCKKFGLDRATRAEAKAPKPSADRAAAQTRPIETVPAAPPSPPFSR
                     RGPNTRALSS"
     gene            709..2007
                     /locus_tag="VLN25_03215"
     CDS             709..2007
                     /locus_tag="VLN25_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09615.1"
                     /translation="MATAEQCFADEYGSLRQGLLTSIFSLVVGLERVFHLDEMEDRGF
                     ARLCGGRRCPSRHTVGGWRRHLSWHEVDAFCRRTCPWHLLRDEDALVSFDEHTIPRWT
                     KKFRIGKGYVTTRNKYMRCEKLFCGYDVGSGRFLSVRAAPGNRGLQELAVPLLEQVLR
                     QSRPRTLHALFDAGAGKSDAGVRALWDLAEQHPKLNVTLRACRYPHRLRGWKQLPSGL
                     FTSIQEPGVCVGAPSKEVRLAETQTVLKGEDPEQAIRTIVCREVRPGPKKDRWHPLYT
                     TAAAAPEAILPIFRARQNQEQSYRVGVHDLGLDAVPCAYDKESPDRQRPRFHRGPLQM
                     IGWLVALVYNAVADLAETLGTDHAGDHVRTLRRKFFHRPGQLYETPAALIVHLDPFSG
                     QDELLPIIDQLNAGRHRLPWLDNRRLVVSLTPRRDSGAEP"
BASE COUNT          414 a          747 c          693 g          443 t
ORIGIN      
        1 ccaaacggtt ttttggggtc tgacggttaa tctctggttg ttacaattct ccctgacggg
       61 atgtcacgga ggattcctca tgacggccac ggacggcgct tttgcacaat ggctttccga
      121 agcgcagctt gccgaagacc ggttgacggc cgagcaacgc ggcctgttgc gggcggcgtt
      181 tgccttccgg cagtgctgcg gccaagatta ctattcgacg cgtttgctca gtcatttcct
      241 gcttcactgc gatgccaatc tcaaagtcgc gcagatcgcc cgcttggtcg ggatcagccg
      301 tcctacggcc tcgcatcagc agggcgtttc ttccaaggaa gcgattcagg cggcacacca
      361 tcgcatggcg ggtcggcccc atggcaagct gctgccgcgt tatgccggac ccatcgccga
      421 gtatattctg agccatgtcg atgccacgcg ttacgacatc ctcgatttca tccagcgcac
      481 ctggaacgtg cgcgtctcca cggtggcctt gcatcacttt tgcaagaagt ttggtctcga
      541 ccgcgccacc cgggccgagg cgaaggcccc caagccgagc gcagatcgcg ctgccgccca
      601 gacgcgtccg atcgaaaccg tgcccgcggc gccgccgtct cccccttttt cgcgacgcgg
      661 acccaatacg cgggcgcttt cctcttgatg ccgcaggcct tgaattggtt ggccacggcc
      721 gagcaatgtt tcgctgacga atacggctct ttgcggcaag gcctgttgac cagcatcttc
      781 tccttggtcg tcggtctgga acgggttttc catctggacg aaatggaaga ccgcggcttt
      841 gcccggctgt gcggcggccg ccgctgtccg tcacgacaca ccgtcggcgg ctggcgtcgg
      901 cacttgtctt ggcacgaggt cgatgccttt tgccgccgca cttgcccctg gcacttgctc
      961 cgcgacgaag acgcgctggt gagcttcgac gagcacacca tcccgcgttg gaccaagaaa
     1021 ttccgcatcg gcaagggcta cgtcaccacc cgcaacaagt acatgcgttg cgagaaattg
     1081 ttctgcggct acgacgtggg cagcggccgt ttcctgagcg tgcgcgctgc gccgggcaat
     1141 cggggtttgc aagagctggc cgtgccgctg ctggagcagg tgctcaggca gagccggcca
     1201 cgcacgttgc atgccttgtt cgacgccggc gcgggcaagt cggacgctgg ggtgcgggcg
     1261 ttgtgggacc tggccgagca gcatcccaag ttgaacgtca cgctacgcgc ctgtcgctac
     1321 ccgcatcgcc tgcggggttg gaaacaactg cccagcggcc tgttcacgtc gatccaggag
     1381 ccgggggtgt gcgtcggcgc tccgtccaaa gaggtccgtc ttgccgaaac tcaaacggtg
     1441 cttaagggcg aagacccgga gcaggcgatt cgcaccatcg tctgccgcga ggtccgaccc
     1501 ggcccgaaga aggaccgctg gcacccgctg tacacgaccg ccgcggcagc accggaagcg
     1561 atcttgccga tctttcgagc tcggcaaaac caggagcaga gctaccgcgt cggcgtccat
     1621 gatcttggcc tggatgcggt gccgtgtgct tacgacaaag agagtcccga ccgacaacgg
     1681 ccgcgcttcc accgcggccc cttgcaaatg atcggctggc tggtggcgtt ggtgtacaac
     1741 gcggtggccg acctggctga gacgctgggc accgaccatg ctggagacca cgtgcggaca
     1801 ctgcgacgga aattctttca ccgaccggga cagttgtacg aaacgcccgc ggccttgatc
     1861 gtccacctcg accccttttc tggtcaggac gagctgctgc cgatcatcga tcaattgaac
     1921 gcgggccgtc atcgtttgcc ctggttggac aaccgccgcc tggtcgtgtc gcttacgccc
     1981 cgccgcgact ccggggccga gccgtagtcc gtcattgtta agcattagag aacaaaaaac
     2041 cgtttggtgc tagggcgctt gctgggcatg ggcgaaatga ccgcccgaaa agagcaagca
     2101 tccagtcgcc ccacagctta ttctgtccac acctccggct cgaccggcgt gctccaagcc
     2161 acaagctcac tccgaagggc tgacttccta gccaccgaca gcgcaccgca gcacctgcac
     2221 ttgccaggca accaaacttc gtaaaggcct agaaaacggc acttccgtga ggtcccgacc
     2281 aacgctgagt ttccaga
//
LOCUS       DATIRT010000132         2721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1098004, whole genome shotgun sequence.
ACCESSION   DATIRT010000132 DATIRT010000000
VERSION     DATIRT010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098004"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VLN25_03220"
     CDS             complement(<1..290)
                     /locus_tag="VLN25_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09616.1"
                     /translation="MKQLLTLLMLSLALTGVARAEQAGGLTWTAPPEWKAQGERPMRA
                     ATYSIPAAKGDSEPAELAVFYFGESSGGGVDANVKRWIEQFQKADGTSAAKG"
     gene            718..1308
                     /locus_tag="VLN25_03225"
     CDS             718..1308
                     /locus_tag="VLN25_03225"
                     /inference="COORDINATES: protein motif:HMM:NF014639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HSQ09617.1"
                     /translation="MSATFNIATQRRLALANDSYLLLLMFVISGILTAAMTLQYAKGE
                     LPCPLCLLERLAMFGVCFGIMLNFRRGFSYQNTGFSLLSAILLLIVSVRQSLLDIYAR
                     PGHEYIGSPVFGIHLPVWSIIIALTILIAYAVKMAIIGGDEYLRSASIDEFPALHRIA
                     DALSAYVILLCVINLVSVVIQCGLGQCHTTGYRLLQ"
     gene            complement(1389..2231)
                     /gene="fghA"
                     /locus_tag="VLN25_03230"
     CDS             complement(1389..2231)
                     /gene="fghA"
                     /locus_tag="VLN25_03230"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004522664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HSQ09618.1"
                     /translation="MSTLTVLSEHACWGGVQGFYRHESTAIGLPMQFGVYLPPQAKSA
                     AVPALFYLAGLTCNEETFAIKAGAQQYAAEHGLIIVTPDTSPRNTGIEGADASWDFGT
                     AAGFYLDAMRRPWDRHWKMETYVAEELRAVIAEHFPVQSDRLGVFGHSMGGHGALTLA
                     LRHPGHYRSVSAFAPIAAPMQAPWGIKAFTGYLGEDRALWREQDATELIARGARMPEC
                     LVDQGTADKFLDEQLHIEALQSACLQAGQPLTLRRHADYDHGYYFIATFVGDHIAHHA
                     RFLG"
     gene            complement(2246..>2721)
                     /locus_tag="VLN25_03235"
     CDS             complement(2246..>2721)
                     /locus_tag="VLN25_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HSQ09619.1"
                     /translation="LIVGVDVNPARRAMAEKFGMTHFVNPLEVEGDIVAYLVDLTGGG
                     ADYSFECVGNVDLMRQALECCHRGWGVSVIIGVAGAGQEIKTRPFQLVTGRVWKGTAF
                     GGARGRTDVPKIVDWYMDGRIKIDELITHRLKLEDINRGFELMKSGESIRSVVVY"
BASE COUNT          466 a          840 c          849 g          566 t
ORIGIN      
        1 tctcccttgg ccgcggaggt gccgtcagcc ttctggaact gctcgatcca gcgcttcacg
       61 ttggcatcca ccccgccgcc actgctttcg ccgaagtaga agacggccag ttcggcgggc
      121 tccgagtctc ccttggccgc gggaatgctg taggtggccg cccgcatggg ccgctcgccc
      181 tgcgccttcc actctggagg agcggtccac gtcaggccgc ccgcctgctc ggctcgcgcg
      241 actcccgtca gagcgaggct caacatcagt aaggtgagta gttgtttcat ggggcgagcg
      301 taactgaaac aatgactatg cgatcgcgcg tgactccggc ggattcgccc tggtacccgg
      361 ctgattgatc tcgtacaaca aacctgaacg gtgactggac tactggttct aagtccggcg
      421 gctggatgac ctctcgcatg caatgaggct tgccaatgat taaggaatgg cgcatgttcg
      481 acgccactgc gtcgacgcca gtgcgccggg gagcgggtca atgatggttg tcgtattcgc
      541 gacgatgtcg ctcgcactga tttgtgtctg gctccgatgg catcggccat cgatcgcgtt
      601 gttcgcttcc agcctggttc ttggcactgg cctttttctc ttcgagatct acagccctga
      661 atacggcttc cgcatgccat ggattcaaac cgagtcgaac gcagggccga cacgatcatg
      721 agcgcgacgt tcaatatcgc aacccagcgc agactcgcgc tggcgaacga cagctatctg
      781 ttgctgctga tgttcgtgat atcggggatt ctgaccgcgg cgatgacgct gcaatatgcg
      841 aagggcgagt tgccgtgccc tttgtgtctg ctcgagcgtc tcgccatgtt cggcgtgtgc
      901 ttcggcatca tgctgaactt ccgtcgaggc ttttcctatc agaacaccgg cttcagcctg
      961 ctgtcagcga tcttgctgct aatcgtttcg gtgcggcaaa gcctgctgga catctatgcg
     1021 cgaccggggc acgaatacat cggcagcccg gttttcggca ttcatctgcc ggtgtggtcg
     1081 atcatcatcg cgctgacgat cctgattgcg tatgcggtca agatggcgat catcggcggt
     1141 gatgagtact tgcggagcgc ctcgatcgat gaattccccg cccttcacag gatcgccgat
     1201 gcattgagcg cttacgtcat tctgctctgc gtcatcaacc tggtgtcggt cgtcattcaa
     1261 tgcggtttgg ggcaatgcca caccaccgga tacaggttgt tgcagtaggt tcgtggcgac
     1321 gttggacggg gatcgtccat agaaaacgaa gtgcccgccg cgtgacgaca ctcgcgacga
     1381 ttcgcgctct agccgagaaa ccgcgcgtga tgcgcgatgt gatcgccgac gaaggtcgcg
     1441 atgaagtagt agccgtggtc gtagtccgcg tggcgacgta gcgtcagcgg ttgaccggcc
     1501 tgcaggcagg cgctctgcag cgcctcgatg tgcagctgct cgtcaaggaa tttgtcggcc
     1561 gtgccctggt cgaccaggca ttcgggcatg cgggcgccgc gggcgatcag ctcggttgca
     1621 tcctgttcgc gccacagtgc gcggtcctct ccgaggtagc ccgtaaatgc cttgatgccc
     1681 cagggtgctt gcatcggcgc cgcgatcggc gcgaaggcgg agacgctgcg atagtggccc
     1741 ggatgccgca aggcgagcgt cagtgcaccg tggccgccca tcgaatgacc gaacacgccg
     1801 agccgatcgc tttgcaccgg gaaatgctcc gcgatgacgg cgcgaagctc ctcggccaca
     1861 taggtttcca tcttccagtg acggtcccat ggccgtcgca tggcatcgag gtagaagcct
     1921 gccgcggtgc cgaagtccca tgacgcgtct gcgccttcga tgcccgtgtt gcgcgggctc
     1981 gtgtcgggcg tgacgatgat gagaccgtgt tcggccgcgt attgctgcgc gcccgccttg
     2041 atcgcgaacg tttcttcgtt gcaggtcagc ccggccagat agaagagcgc cggcaccgct
     2101 gcgctcttcg cctgcggcgg caaataaacg ccgaactgca tcggcagtcc gatcgccgtc
     2161 gactcatggc gatagaagcc ctgcacgccg ccccagcacg cgtgctcgct caggaccgtg
     2221 agggtcgaca tctcagtaga cgatgtcagt agacgaccac ggagcggatc gactcgccgc
     2281 tcttcatcag ctcgaacccg cgattgatgt cctcgagctt cagccggtgg gtgatgagct
     2341 cgtcgatctt gatgcggccg tccatgtacc agtcgacgat cttcgggaca tcggtgcggc
     2401 cgcgtgcgcc gccgaaagcg gtgcctttcc atacgcggcc ggtcacgagc tggaatggcc
     2461 gcgtcttgat ttcctgtcct gcgcccgcaa cgccgatgat gaccgaaacg ccccagccac
     2521 ggtggcagca ttcgagcgcc tgtcgcatca ggtccacgtt gcccacgcat tcgaagctgt
     2581 agtcggcgcc gccgccggtg aggtcgacca ggtaagcgac gatgtcgccc tcgacctcca
     2641 ggggattgac gaagtgcgtc atgccgaatt tctcggccat cgcccggcgc gcgggattga
     2701 cgtcgacgcc gacgatcagg t
//
LOCUS       DATIRT010000133         6836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_705012, whole genome shotgun sequence.
ACCESSION   DATIRT010000133 DATIRT010000000
VERSION     DATIRT010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6836
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705012"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VLN25_03240"
     CDS             <1..295
                     /locus_tag="VLN25_03240"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HSQ09620.1"
                     /translation="DALERIEAEHLAMAEVWRRIDATLARISAGDPALLDPQTIAQFA
                     TRYDQHIDIEENVVLPALRRRLTAADWSSVGRAMAERRGLDWSETPARGDTLL"
     gene            complement(315..782)
                     /locus_tag="VLN25_03245"
     CDS             complement(315..782)
                     /locus_tag="VLN25_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HSQ09621.1"
                     /translation="MINRRRLFLVGLTVACVPVLAHHGWSSFDQDKPLYLSGTVKSVR
                     WQNPHVEFVLVVAPGLVKPADLPGRKAPTQSQSVDGSAVLGKAAVPAQAAGDWEIELA
                     PLSRVSAWGVAELKPGDRVEVVGYALASGDKRVLRVEYLFAQGKAFGMRSSPQ"
     gene            complement(815..1267)
                     /locus_tag="VLN25_03250"
     CDS             complement(815..1267)
                     /locus_tag="VLN25_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09622.1"
                     /translation="MIDWYTLEGWPLAEAIRHSVWAYPALETLHIAALATLVGSLLVL
                     ELRVFGAQPALSLTALGRLAVRTALTGFTLAVLSGSLLFLSAATEIAANPAFRVKLVL
                     ILMAGGNALVFHARDSLRRHDTVARLQAGLSLTLWFGVIAAGRLIAYI"
     gene            complement(1298..2839)
                     /locus_tag="VLN25_03255"
     CDS             complement(1298..2839)
                     /locus_tag="VLN25_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HSQ09623.1"
                     /translation="MSDPLIIAMSGTTEIGLLPALANRHGCITGATGTVKTVTLQKLA
                     ESFSRIGVPVFMADVKGDLTGIAKAGALGDKMKKRLEEHRLPEPKWGACPVTLWDVWG
                     EQGHPVRATVSDMGPLLLSRLLGLNETQEGVLSIVFKAADDNGLLLLDLKDLRAMVQF
                     IGDNAAKVKTEYGNVSSASIGAIQRGLLLIEEQGGDRFFGEPMLNIDDLMQTADGQGV
                     VNILAADKLMNNPRLYSTFLLWLLSELFENLPEVGDREKPKLVFFFDEAHLLFSEAPP
                     ALLEKVEQVVRLIRSKGVGVFFVTQNPLDIPEKVLGQLGNRVQHALRAFTPRDQKAVK
                     SVAETMRANPKIDASKAIMELGVGEALVSLLDAKGTPGITERAWVLAPGSQLGPITPE
                     DRRKLIAGSVVGSTYEKTIDRESAHEKLKARTGGGETLPAGKEAGEPAASGQAPGQSG
                     GGGLMGSLGDLLFGSTGPRGGRREGMVDAMAKSAARSVGSSLAREITRGVLGGLLGGS
                     TRKRR"
     gene            2978..3703
                     /locus_tag="VLN25_03260"
     CDS             2978..3703
                     /locus_tag="VLN25_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HSQ09624.1"
                     /translation="MAGHSKWANIQHRKNRQDVKRGSLWTKIIREVTVAARDGGPDPG
                     MNPRLRLALDKARLGNVPKDKLTNAIQRGAGRLEGASYEEIRYEGYGIGGAALMIDCM
                     TDNRTRTVAEVRHALSKNGGNLGTDGSVAFNFKHCGQIVFAPGTSEEKVMDAALEAGA
                     EDVISNDDGTIEVITAPANFESVREALDRSGLKPEIAEVTMKPLAETVLSGDDAQKMQ
                     RLIDALDDLDDVQQVYSTAALEE"
     gene            3726..5003
                     /gene="purD"
                     /locus_tag="VLN25_03265"
     CDS             3726..5003
                     /gene="purD"
                     /locus_tag="VLN25_03265"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HSQ09625.1"
                     /translation="MKVLVVGGGGREHALAWRLAQSPRVQRVYVAPGNGGTVRDRHLR
                     NVPHDSHESLAAFVKSEDIALTLVGPEAPLASGIVDVFRLHGLRVFGPTRAAAQLESS
                     KDFAKAFMQRHGIPTADYRTFTDAAEARAYVRQRGAPIVVKADGLAAGKGVVVAGTVA
                     EAEAAIDSMLLGNTLGQSGARVVVEDFLQGEEASFIVMSDGRNALALATSQDHKRLLD
                     GDLGPNTGGMGAYSPAPIVTPTIHARVMREIILPTIQGMAADGVPYTVFLYAGLMIDS
                     HGQARTLEFNCRMGDPETQPIMARLKTDFTVLLDAAIDGRLDQVEAEWDRRTALGVVV
                     AAAGYPDAPRKGDAIARLPEDRDNCITFHSGTAFSGERLITSGGRVLCVTALGDTVRA
                     AQKAAYDAVEMVEFDGMQYRHDIGHRALRPRTL"
     gene            5167..>6836
                     /locus_tag="VLN25_03270"
     CDS             5167..>6836
                     /locus_tag="VLN25_03270"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HSQ09626.1"
                     /translation="MSSAPYTVEQHVQLKRVSSISPSPDGTWLAVAVQRLDREGAKYV
                     SDLWKVPTDGAAPTQLTRGDSKDAAPCFRTDGALGFLSNRQPTEVKPDEDADKRMQVW
                     ILPAGGGEPQQLTDEPLGVESFRFAKSAAVLALFAPVLPDVEHDKQRETASERRKKHS
                     SARHFRQQPVRHWDHWLHQNDNMADTHLIVYAADGTQRVDLTPEARHELSIEPALELS
                     SDGRQVAVTWQTPGQDREIDTAIRLIDTATKSARTLGGATNTNNDVPLLSPDGKTLSV
                     VRSTRSSTAAMRPTLTLIDVATGTMRELGTAFDAWPHPGAWTADGKQIVFTCDLDGQV
                     PVFTLDVAADKVDRITTARSSGAHSDLQVLKDGRIVGIRSTFREPPEAFVVTNQPDSP
                     PQVLARLSGFEFPDWVDVEHCTATSTDGASIQYFVARPAKSQGKLPTLLWIHGGPIGM
                     DADAWHWRWNPLLAVAQGYVVVLPNPRGSTGFGQDFVQGIWGNVWGKQCYEDLMAVTD
                     AAERRPDVDPERVMAMGGSFGGYMTNWIGTQTQRFKCLITHACVTTMA"
BASE COUNT         1238 a         2290 c         2202 g         1106 t
ORIGIN      
        1 cgatgcgctc gaacgtatcg aggccgagca tctcgcgatg gcggaggtgt ggcgtcggat
       61 cgatgccacg ctggcacgta tcagcgcagg tgatcccgca ctcctcgatc cgcaaacgat
      121 tgcgcagttc gcgacccgct acgaccagca catcgacatc gaagaaaacg ttgtcctgcc
      181 cgcgctgcgt cgccggctga ctgcagccga ctggtcgtcg gtcggtcgtg caatggccga
      241 acggcgcgga ctcgactgga gtgaaactcc cgcgagaggc gatacgctgc tctaggttcg
      301 gcgcatctcc ccgactactg cgggctcgat cgcatgccga aggccttgcc ctgggcaaac
      361 aaatactcga cgcgcagcac gcgcttgtct ccactggcca gcgcgtagcc gacgacttcc
      421 acgcggtctc cgggcttcag ttcagccaca ccccatgccg acacgcgtga cagcggcgcc
      481 agttcaattt cccagtcgcc ggcggcctgt gccggcaccg cggctttccc gagcacagcg
      541 ctcccgtcga ccgactggct ttgcgtcggc gcctttcgac ccggcagatc ggcaggcttc
      601 accaacccgg gtgcgacgac aagtacgaat tcgacgtgcg ggttctgcca gcgcacggac
      661 ttgaccgtgc ccgaaaggta aagcggcttg tcctgatcga aactcgacca gccatggtgc
      721 gcgagtaccg ggacgcaggc gacagtcaga ccgacgagga aaagacgacg acgattgatc
      781 atttcaagac tccgctcagt gggccggtcc gaggttaaat gtacgcaatc agccggccgg
      841 ccgcgatcac gccgaaccag agcgtgagcg acaggcctgc ctgcaggcgc gcgacggtat
      901 cgtgacgtcg caacgaatcc cgcgcatgaa acaccagcgc attgcctcca gccatcagga
      961 tcagtaccag tttgacccgg aacgcgggat tggcggcgat ttcagttgcg gccgagagga
     1021 agagtagcga cccgctcagc accgcgaggg taaagcctgt caacgccgtg cgtaccgcaa
     1081 gtcgccccag cgcggtcagc gaaagtgcgg gctgcgcccc gaaaactcgc agctccagga
     1141 ccagcaggct gcccacgagc gttgccaggg ccgcgatgtg cagtgtctcg agtgccgggt
     1201 aggcccagac ggagtggcgg atcgcctccg ccaacggcca gccttcgagc gtgtaccagt
     1261 cgatcacctg tcatcgcccg tcgcgcggat tgccggttca gcgtcgcttg cgagtcgaac
     1321 caccgagcag tccgcccagt acgccacgcg tgatttcgcg cgccagcgat gagccaaccg
     1381 atcgcgctgc gctcttcgcc atggcatcga ccatgccttc tcgtcggccg ccgcgcgggc
     1441 cggtcgagcc gaaaagcagg tcgcccaggc tgcccatcag gccgccacct cccgattgtc
     1501 cgggtgcctg tccggacgcg gcgggctccc cagcctcctt gccggcgggc agggtctcgc
     1561 cgccgcccgt gcgggccttg agcttctcgt gggcggactc acgatcgatc gtcttctcgt
     1621 aggtgctgcc caccaccgag ccggcgatca gtttcctgcg atcctccggc gtgatgggac
     1681 cgagttggga gcccggcgcg agcacccatg cgcgctcggt gatgccggga gtgcccttcg
     1741 catccaggag cgagaccagg gcttcgccga cacccagctc catgatcgcc ttcgacgcat
     1801 cgatcttggg attggcgcgc atcgtttcgg cgaccgactt gaccgccttc tgatcgcgtg
     1861 gcgtgaacgc gcgcagcgca tgctgcacgc ggttgccgag ctggcccaga accttttccg
     1921 gaatgtcgag cggattctgc gtgacaaaga agaccccgac gcccttcgag cgaatcagcc
     1981 gcaccacctg ctcgaccttt tccagcagcg ccggcggcgc ttccgagaac agcagatgcg
     2041 cttcgtcgaa gaagaacacg agcttcggct tctcgcggtc gcccacttca ggcaggttct
     2101 cgaacagttc cgacagcagc cacagcagga aggtcgaata cagccgcggg ttgttcatca
     2161 gcttgtcggc agcgaggatg ttgaccaccc cctggccatc cgcggtctgc atcaggtcgt
     2221 cgatgttgag catcggctcg ccaaaaaagc ggtcaccgcc ctgttcctcg atcagcaaca
     2281 ggccgcgctg gatcgcaccg atcgaggccg acgacacgtt gccgtattcg gtcttgacct
     2341 tggccgcgtt gtcgccgatg aactggacca tcgcgcgcag atccttgagg tccagcagca
     2401 gcaggccatt gtcgtccgcg gccttgaaga cgatggaaag cacgccttcc tgcgtctcgt
     2461 tcagtcccag caggcgcgaa agcagtaacg gtcccatgtc ggagacggtt gcgcgaacgg
     2521 ggtgtccctg ctcgccccag acgtcccaca gggtgaccgg gcacgcaccc cacttcggct
     2581 ccggcaggcg gtgctcctcc aggcgcttct tcatcttgtc gccgagcgcg ccggccttgg
     2641 cgatcccggt gaggtcgccc ttcacgtccg ccatgaacac cggtacgccg atgcgcgaaa
     2701 agctctccgc cagcttctgc agcgtgacgg tcttgacggt gccggtggca ccggtgatgc
     2761 aaccgtgccg gttcgcgagt gcaggcagca gcccgatctc ggtggtgccc gacattgcga
     2821 tgatcagtgg atcgctcatg ttctttcctc tcgatgccga ccgcagtcta gccaaagcgc
     2881 tggccgaatc acgggcgggt ggcgggcgtt cgcggtttgc ggtccgctag actccggtcc
     2941 tttccgcatc agtcaccgca acatcgaaag cagcatcatg gccggccatt ccaaatgggc
     3001 caacatccag catcgcaaga accgccagga cgtcaagcgc ggctcactct ggaccaagat
     3061 catccgcgag gtcactgtcg ccgcgcgcga cggcggacct gaccctggca tgaacccgcg
     3121 cctgcgcctg gcgctggaca aggcgcggct cggcaatgtc ccgaaggaca agctcaccaa
     3181 tgcgatccag cgcggcgccg gcaggctcga gggcgccagc tatgaagaaa tccgctatga
     3241 aggctatggc atcggcggcg cggcactgat gatcgattgc atgacggaca accgcacccg
     3301 gaccgtggcc gaagtacgcc atgcgttgtc gaagaatggc ggaaacctcg ggaccgacgg
     3361 ctcggtggcg ttcaatttca agcactgcgg ccagatcgtg ttcgcgcccg gcacctcgga
     3421 agagaaagtg atggacgccg cgctcgaggc cggtgccgaa gacgtaatca gcaacgacga
     3481 tggcacgatt gaggtcatca ctgcgccggc caacttcgag tccgtgcgcg aggcgctgga
     3541 ccggtcgggg ctgaagccag agatcgccga agtgacgatg aagccgcttg ccgagaccgt
     3601 gctgagcggc gacgatgcgc agaaaatgca gcggctgatc gacgcgctcg acgatctcga
     3661 cgacgtgcag caggtctaca gcacagccgc cctcgaggaa tgagcgtgca ccggggccca
     3721 cggcgatgaa ggtcctggtc gtcggcggcg gaggccgcga gcacgcgctt gcatggcgac
     3781 tggcacagtc tccgcgcgtg cagcgtgtct acgttgcgcc gggcaacggt ggcacggtac
     3841 gcgaccggca tttgcgcaac gtgccgcacg acagccacga gtcgctggca gccttcgtga
     3901 agtccgagga catcgcgctg acgttggtcg gaccggaagc gcccctggcc agcggcatcg
     3961 tcgatgtctt ccgtctccac ggactgcggg tcttcggacc gacgcgggcg gccgcacaac
     4021 tggaaagctc gaaggatttc gccaaggcct tcatgcagcg ccacggcatt ccgaccgccg
     4081 attaccgaac tttcaccgac gccgccgaag cgcgggccta cgtgcgccag cgcggcgcgc
     4141 cgattgtggt caaggccgac ggcctggcgg ccggcaaggg cgtcgtcgtt gccggcacgg
     4201 tcgccgaggc agaagcggcg atcgactcga tgctgctcgg caatacgctc gggcaaagcg
     4261 gggcacgcgt ggtcgtcgag gacttcctgc aaggcgagga agcgagcttc atcgtgatga
     4321 gcgatggccg caatgcattg gcgctcgcca ccagccagga tcacaagcga ctgctcgacg
     4381 gtgacctcgg cccgaacacc ggcggcatgg gagcctattc gcccgcgccg atcgtgacgc
     4441 cgacgatcca tgcgcgggtg atgcgcgaga tcatcctccc gacaatccag gggatggccg
     4501 ccgacggcgt tccgtacacc gtcttcctgt atgccggcct gatgatcgac agccatggcc
     4561 aggcccgcac gctcgagttc aactgccgca tgggcgatcc cgaaacgcag ccgatcatgg
     4621 cgcgtctgaa gaccgacttc accgtgctgc tcgacgcggc gatcgacggt cgactcgatc
     4681 aggtcgaagc cgaatgggat cgacgcaccg cactgggcgt ggtggtggct gcggcgggct
     4741 atccggacgc accacgcaaa ggcgacgcga tcgcgcgact gcctgaggac cgcgacaact
     4801 gcatcacgtt ccattccggc accgcgttca gtggtgagcg actcatcacg agcggcggcc
     4861 gggtcctgtg cgtgactgcg ctcggggaca cggtgcgcgc ggcgcagaaa gccgcctacg
     4921 acgccgtcga gatggtcgag ttcgacggca tgcagtaccg gcatgacatc ggccatcggg
     4981 cgctgaggcc ccggacgctg tagtgccccg ctgtcgcccc ccgctgtagc cctcagtgcc
     5041 tcccttgtcg atttctcttc tgcggctgaa ctgtcgcacc gtccggcccc cacgcaggtg
     5101 cggcctaagg ctttcgtcca caatgaccga cttttcggct acccagaccc cggacctgac
     5161 cgaaccatga gctccgcccc ctataccgtt gaacagcacg tccagctgaa acgtgtttcg
     5221 tcgatctcac cttcccccga cgggacgtgg ttggccgtcg cggtgcaacg cctcgaccgg
     5281 gaaggcgcga agtacgtctc cgacctgtgg aaggtgccca ccgacggcgc cgcaccgact
     5341 caactgacac gcggcgacag caaggatgcg gccccgtgtt tccgcactga cggcgcgctc
     5401 gggttcctct ccaaccgcca gccgaccgaa gtcaagccgg acgaagacgc ggacaagcgc
     5461 atgcaggttt ggatactgcc ggccggaggc ggcgagccgc agcagctgac cgatgagccg
     5521 ctcggggtcg agtcatttcg cttcgcgaaa agcgcggccg tgctggcact gttcgcgccg
     5581 gtattgccgg acgtggaaca cgacaagcag cgcgagaccg ccagtgaacg gcgcaagaag
     5641 cactcgagcg cgcgacactt ccggcagcag ccggtgcgcc actgggacca ttggctgcac
     5701 cagaacgaca acatggccga tacgcacctg atcgtctacg cggccgacgg gacccaacgc
     5761 gtcgacctga cgcctgaagc gaggcacgag ctgtcgatcg agccggcact ggagctctcg
     5821 tcagacggca ggcaggttgc cgtcacctgg cagacgcccg gacaggatcg ggagatcgac
     5881 accgcgatcc ggctgatcga caccgcgacg aagtcggcgc ggacgcttgg cggtgctacc
     5941 aacacgaaca acgatgtgcc attgctttct ccggacggca agacgctgtc cgtcgtgcgc
     6001 tcgacccgct cttcgacggc ggccatgcgg ccgacgctga cgctgatcga cgtggccacc
     6061 gggacgatgc gcgaactggg cacggcgttc gacgcgtggc cccatccggg cgcctggacg
     6121 gccgatggaa agcagatcgt gttcacctgc gacctggacg gtcaggtgcc cgtgttcacg
     6181 ctcgacgtcg ccgcggacaa ggtcgatcgc atcaccacgg cacgcagcag cggcgcgcac
     6241 agcgatttac aggtgttgaa ggacggccgc atcgttggca tccgttcgac tttccgcgag
     6301 ccgcccgagg ccttcgtcgt cactaaccag ccggactcgc cgccccaggt gctggcacgc
     6361 ctgtccggct tcgagttccc cgattgggtc gacgtcgagc actgcacggc gacctcgacc
     6421 gacggtgcgt cgatccagta ctttgtcgcc cgaccggcca agtcgcaggg caagctgccg
     6481 acgctgctgt ggatccacgg cggcccgatc ggcatggatg cagacgcctg gcattggcgc
     6541 tggaatcccc tgctcgcggt ggcacagggg tatgtcgtcg tgctgccgaa tccgcgcggc
     6601 tcgaccgggt tcggccagga cttcgtgcaa ggcatctggg gcaacgtctg ggggaagcag
     6661 tgttacgaag acctgatggc cgtgaccgac gctgcggaac ggcgtccgga cgtcgatccg
     6721 gagcgtgtga tggcgatggg cggcagcttc ggtggctaca tgaccaactg gatcggcacg
     6781 cagacccagc gcttcaagtg cctgatcacg cacgcctgtg tgacgacaat ggccca
//
LOCUS       DATIRT010000134         1829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_802997, whole genome shotgun sequence.
ACCESSION   DATIRT010000134 DATIRT010000000
VERSION     DATIRT010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802997"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="VLN25_03275"
     CDS             <1..1063
                     /locus_tag="VLN25_03275"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814928.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HSQ09627.1"
                     /translation="DDGTGGFELDQGKLKKTIRTAMRMLDNVIYINYYAVPKARNSNV
                     RHRPVGLGIMGFQDCLHMMRTPYASEGAVEFSDRSMEAVCYYAYWASTELAEERGRYS
                     SFAGSLWDRGILPQDSLGLLKEQRGGHVDVDMSASMEWDALRGRIRQFGMRNSNCVAI
                     APTATISNIIGVDACIEPTFQNVFVKSNLSGEFTVINEHLVHDLKRLGLWDEVMVSDL
                     KYFDGSLAKIDRIPAEIRSLYATAFEIEPTWLIECAARRQKWIDQAQSLNIYMAGVSG
                     KKLDETYRLAWRRGLKTTYYLRTIGATHAEKSTSTRTGQLNSVSSDMGASVAAMEADG
                     KACMLKPGDAGFADCEACQ"
     gene            1097..>1829
                     /locus_tag="VLN25_03280"
     CDS             1097..>1829
                     /locus_tag="VLN25_03280"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HSQ09628.1"
                     /translation="MLAWDDNTAPLASKPTLPTAAARPVQPGPAVDAPELRRVNVADK
                     RIINGATDVNQLVPFKYKWAWEKYLATCANHWMPQEVNMSRDIALWKDPNGLTEDERR
                     LVKRNLGFFVTADSLAANNIVLGTYRHITAPECRQFLLRQAFEEAIHTHAYQYIVESL
                     GLDEGEIFNAYHEVDSIRAKDEFLIPFIDTLTDPHFRKGTLENDQALLKSLIVFACLM
                     EGLFFYVGFTQILALGRQNKMTGAAE"
BASE COUNT          375 a          586 c          560 g          308 t
ORIGIN      
        1 ggatgacggc accggcggat tcgagctcga tcagggcaag ttgaagaaga ccatccgtac
       61 cgccatgcgg atgctcgaca acgtcatcta catcaactac tacgccgtgc cgaaggcgcg
      121 caactccaac gtgcgtcacc gcccggtcgg actcggcatc atgggcttcc aggactgcct
      181 gcacatgatg cggacgccct acgcgtccga aggcgcagtc gagttctccg atcgcagcat
      241 ggaggcggtc tgctactacg cctactgggc gtcgacggag ctggccgagg agcgcgggcg
      301 ctattcctcg tttgccgggt ctctttggga tcgcggcatc ctgccgcagg actcgctcgg
      361 tcttctcaag gagcagcgcg gaggccatgt cgacgtcgac atgagtgcct cgatggagtg
      421 ggacgcattg cgcgggcgaa ttcgtcagtt cggcatgcgc aattcgaact gcgtggccat
      481 cgcgccgacc gcgacgatct caaacatcat cggggtcgac gcctgcatcg agcccacctt
      541 ccagaacgtt ttcgtgaagt cgaacctctc gggggagttc accgtcatca atgagcacct
      601 cgtccatgac ctcaagcggc tcggcttgtg ggacgaagtg atggtttcgg acctgaagta
      661 cttcgacggc tcgctggcga agatcgatcg gataccagcg gagattcgca gcctctacgc
      721 gaccgcgttc gaaatcgagc cgacctggct gatcgaatgt gccgcgcggc gccagaagtg
      781 gatcgaccag gcgcagtcac tcaatattta catggctgga gtctccggca agaaactcga
      841 cgagacctac aggctggcct ggcggcgggg cttgaagacg acctactacc tgcggacgat
      901 cggcgcgacc catgccgaga agtcgacctc gacccgtacc ggccagctga attccgtgtc
      961 gtccgacatg ggggcatcag tcgcagccat ggaagcggac ggcaaggcct gcatgctcaa
     1021 gccgggcgac gcgggattcg cagactgcga agcctgccag taacgcacaa cacgaagtca
     1081 acgggagaag atcagcatgc tggcgtggga cgacaacacc gcacctctgg cctcgaagcc
     1141 aacgctgccc acggccgccg cgcggccggt gcagcctgga ccggccgtcg atgcacccga
     1201 gctgcggcgg gtcaacgtgg ccgacaagcg gatcatcaac ggcgccaccg acgtcaacca
     1261 gctcgtcccc ttcaagtaca agtgggcctg ggagaagtat ctcgccacct gcgccaacca
     1321 ctggatgccg caggaagtga acatgtcgcg cgacatcgcg ctctggaagg atccgaacgg
     1381 actaaccgag gacgagcgcc ggctcgtcaa gcgcaacctc gggttcttcg tcacggccga
     1441 ctcgctggcg gccaacaaca tcgtgctcgg cacctaccgc cacatcacgg cgccggagtg
     1501 ccggcagttc ctcctccgcc aggccttcga ggaggcgatc cacacccacg cctaccagta
     1561 catcgtcgaa tccctcgggc tggacgaggg cgagatattc aatgcctacc acgaggttga
     1621 ctcgatccgg gccaaggacg agttcctgat cccgttcatc gacacgctga ccgatcctca
     1681 cttcaggaaa ggaacgctgg aaaacgacca ggcgctactg aagagcctga tcgtcttcgc
     1741 ctgtctgatg gaggggctgt tcttctacgt cggcttcacg cagatccttg cgctgggccg
     1801 ccagaacaag atgaccgggg ctgccgagc
//
LOCUS       DATIRT010000135         1590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_311692, whole genome shotgun sequence.
ACCESSION   DATIRT010000135 DATIRT010000000
VERSION     DATIRT010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311692"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="VLN25_03285"
     CDS             complement(<1..1070)
                     /locus_tag="VLN25_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09629.1"
                     /translation="MLDKIFGSPLHEHADPAQRVLGMAALPPDSKVLAQLLGSDPSTD
                     VRSAAATRCTDPVVLIRALQAETEPSVRSAIASSLGKLLAATADIGIVRSLLAAPECP
                     DAVRAQIALHTQDEQRRRDALNGMADEDALVDVALGAEHASVRIAAAERVHAPEPLRR
                     LLTGARDKDRGVARLARERLDAINRRIENADAADAILREAEALVLQPGPIVTAAVELD
                     RRWKALVLGDDEVRRARWDAVGRMMQQRFDRELDEQRAHARFEQRLGAWLAALQPPPA
                     NELPVLREQLIALRTEAADEKDSRALARLEQAERQLLQWEQVAPALAAAEALVVEAEE
                     LATGTPIDDAQLPARWQALDLAA"
     gene            complement(1221..>1590)
                     /locus_tag="VLN25_03290"
     CDS             complement(1221..>1590)
                     /locus_tag="VLN25_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09630.1"
                     /translation="PTFDLITAPQAGGYFIGDYFGLAASGNVFIPVYVRTTGSTTNRT
                     DVFAVFARSIASSATRAANSVTGNTAAANSPAVNTTTAHPEDDATRQRASDHLVRVME
                     RRIPGWTSWRAETAGSRIDR"
BASE COUNT          237 a          530 c          540 g          283 t
ORIGIN      
        1 gccgcgagat ccagcgcctg ccagcgggcc ggcagttgcg catcgtcgat cggcgttccg
       61 gtcgccagct cctcggcttc caccaccaga gcctcggcgg cggccagcgc cggagccacc
      121 tgttcccact gcaagagctg tcgttcggcc tgctccagcc gtgccagggc gcggctgtct
      181 ttttcgtcgg ccgcttcggt gcgcagcgcg atcaactgtt ctcgcaacac gggcagctca
      241 tttgccggag gaggttgaag cgcggccagc cacgcgccga gtcgctgctc aaaccgtgcg
      301 tgcgcgcgtt gctcatcgag ttcgcgatcg aagcgttgct gcatcatccg accgaccgcg
      361 tcccagcggg cgcgacggac ttcgtcgtcg cccagaacca gcgccttcca gcgtcgatcg
      421 agttcgacgg cagcggtcac gatcgggccg ggctgcaaca caagcgcctc ggcctcccgc
      481 aggattgcat cggctgcatc ggcgttctcg atgcgccggt tgatcgcgtc gagccgctcg
      541 cgcgcgaggc gagccacgcc gcggtccttg tcccttgctc ccgtcagcaa gcggcgcagt
      601 ggttcgggcg catgcacgcg ctccgcggca gcgatccgca ccgacgcgtg ctctgcgccg
      661 agcgcgacgt ccaccagcgc atcctcgtcg gccatgccgt tcagagcgtc gcggcgccgc
      721 tgctcgtcct gcgtatgcag cgcgatctgc gcgcggactg catccgggca ctctggtgca
      781 gcaagcaggg aacgcacgat gccgatgtcc gccgttgccg ccagcagctt gcccaacgaa
      841 gaggcgatgg ccgaacgtac ggacggctcg gtctccgcct gcaaggcgcg aatgagcacg
      901 accggatcag tacagcgtgt ggccgcagcg ctgcggacat cggttgacgg atcggagccg
      961 agcagctgcg ccagcacttt cgaatccggc ggcagcgcag ccatgccgag cacgcgttgc
     1021 gccgggtcag cgtgttcgtg cagaggggaa ccaaagatct tgtcgagcac gtcgaatctg
     1081 cgttgggaag tcgggatcag tcggatgtat ttgtcgcttc agtggggatg gtaccgagtg
     1141 cggcgccttg gtcttcggct gccgacctct caaggccccg ccggggcatg aggaccttcc
     1201 ttcaggaaca cttccggact ttaccggtcg attctggagc ctgctgtctc ggcgcgccag
     1261 ctcgtccatc ccgggatgcg ccgttccatc acgcggacaa gatgatcgct cgcgcgctgg
     1321 cgggtggcgt cgtcctcggg gtgtgccgtc gtcgtgttca ccgccggcga atttgctgcc
     1381 gccgtgtttc ccgtcactga gttcgcagca cgtgtagcgc tggacgcgat cgagcgcgcg
     1441 aagacggcaa atacgtccgt gcggttggtc gtgctgccgg tggtgcgtac gtacaccggg
     1501 atgaacacgt tgccactggc cgcgaggccg aagtagtcgc cgatgaagta gccgccggcc
     1561 tggggcgcgg tgatgaggtc aaaggtcggt
//
LOCUS       DATIRT010000136         1776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1884542, whole genome shotgun sequence.
ACCESSION   DATIRT010000136 DATIRT010000000
VERSION     DATIRT010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1884542"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VLN25_03295"
     CDS             <1..623
                     /locus_tag="VLN25_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSQ09631.1"
                     /translation="IAYFLDGFGQTLWGSDIYKIDTGLPKTPLLVLEQIFDGGILINT
                     EDMVAAAIAGLLVLALALFFQKTPTGRALRAVVDDHQAAQSIGIPLNRIWVIVWTVAG
                     FVALVAGMIWGSKLGVQFSLSLIALKALPVVILGGFTSVPGAILGGLIIGVGEKFAEV
                     FLGPVLVREFDLAGGGIENWFAYVLALLFLLVRPEGLFGEKHIDRV"
     gene            742..>1776
                     /locus_tag="VLN25_03300"
     CDS             742..>1776
                     /locus_tag="VLN25_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSQ09632.1"
                     /translation="MLYRENGQFKTSYAEDQQILPIVQDRWFMLALLAFAYLAMPAFA
                     TEYLFLSLVVPFLILSIAAMGLNILVGYCGQISLGTAAFMAVGAYAAYNFVIRVPALN
                     FIVVLALSGGIAALVGVLFGIPSLRIKGLYLAVATLAAQFFIDWLFARVKWFTNYSSS
                     GSVSTPPIEIFGWVVDSPVEKYLFVLTIAVVMTLAAKNLVRGHIGRSWMAIRDMDVAA
                     EVIGIRPLYAKLSAFAVSSFYAGVAGALWGFIRLGAWEPLAFNVNLSLNLLFMIIIGG
                     MGSLLGSYFGAAFIVILPILLNQVPGTIGIEMSTALASHLELMIFGSLIVFFLIVEPH
                     GLARLWAIGKE"
BASE COUNT          274 a          530 c          566 g          406 t
ORIGIN      
        1 gcattgcgta cttcctcgac ggcttcgggc agaccctttg gggcagcgat atctacaaga
       61 tcgacaccgg gctgcccaag acgccgttgc tggtgctgga gcagatcttc gatggcggca
      121 tcctgatcaa caccgaagac atggtagcgg cggccatcgc gggcttgctc gtgctggcgc
      181 tcgcgctgtt cttccagaag acgcccaccg gtcgcgccct gcgcgcggtc gtggacgatc
      241 accaggccgc gcaatcgatc ggcatcccgc tgaaccgcat ctgggtcatc gtctggaccg
      301 tcgccggatt cgtggcgctg gtcgccggca tgatctgggg ttcgaagctg ggcgtccagt
      361 tttccctgtc gctgatcgcg ctgaaggctc tgccggtcgt cattctgggc ggcttcacgt
      421 ccgttccagg ggccatcctc ggcggactca tcatcggtgt cggggagaag tttgcagagg
      481 tatttctcgg tcctgtgctc gtgcgcgaat tcgacctggc ggggggcggc atcgagaact
      541 ggtttgccta cgtgctggcg ctgctcttcc tgctggttcg ccccgaaggg ctgtttggtg
      601 aaaaacatat cgaccgcgtg tagcgggcga aaggttttcg gagaggctaa tgtgagcgaa
      661 gcgaacgtta agcgagtaca gcgagcgggc cggaaccaaa aacatatcga ccgcgtatag
      721 cgacgaggcc tgacgtccac catgctctac cgcgaaaacg gccagttcaa gacctcgtac
      781 gccgaggacc agcagatcct gccgatcgtg caggaccgct ggttcatgct ggcgctgctg
      841 gcatttgcct atctggcgat gcccgcgttc gccacggagt acctgtttct ctcccttgtc
      901 gtgccgttcc tgatcctttc gatcgcggcc atgggcctga acatcctggt ggggtactgc
      961 gggcagatct cgctgggaac tgccgccttc atggccgtcg gcgcctacgc tgcctataac
     1021 ttcgtcatac gcgtgccggc gctcaacttc atcgtggtgc tggcgctctc tggcggcatc
     1081 gcggcgctgg tcggcgtgct ctttggtatt ccgagcctgc gcatcaaggg gctgtatctg
     1141 gccgtcgcca cgctggctgc gcagttcttc atcgactggc tgttcgcgcg cgtaaagtgg
     1201 ttcaccaact actcctcgtc ggggtcggtg tcgacgccgc cgatcgagat cttcggctgg
     1261 gtcgtcgact ctccggttga gaagtatctg ttcgtcctga cgatcgccgt cgtgatgacg
     1321 ttggcagcga agaatctcgt gcgcggacat atcggccgaa gctggatggc catccgtgac
     1381 atggacgtcg cggccgaggt gatcggcatt cggccgctgt acgccaagct gtcggcattc
     1441 gcggtcagtt cgttctacgc cggcgtggcc ggcgccctat gggggtttat ccggctcggc
     1501 gcctgggagc cactggcgtt caatgtgaac ctgtcgttga atctgctgtt catgatcatc
     1561 atcggcggga tgggttcact gctaggctcg tatttcggcg ccgcgttcat cgtcatcctc
     1621 ccgatcctgt tgaatcaggt tccgggaacc atcggaattg aaatgtcgac ggcgctcgct
     1681 tcgcatctgg agctcatgat cttcggcagc ctgatcgtgt tcttcctgat cgtcgaaccg
     1741 cacggccttg cgcggctgtg ggcgatcggc aaggag
//
LOCUS       DATIRT010000137         4848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_508032, whole genome shotgun sequence.
ACCESSION   DATIRT010000137 DATIRT010000000
VERSION     DATIRT010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4848
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508032"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..1053)
                     /locus_tag="VLN25_03305"
     CDS             complement(103..1053)
                     /locus_tag="VLN25_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HSQ09633.1"
                     /translation="MQLGWLALVITLPTSPSAVRVRIWRAMKASGCGALRDGVYLLPE
                     RPDCAQVFDGLADEVRQADGEATQLAFGARDAEQQRQFEALFDRSDEYKAIERDLARA
                     RRELRTAEEVPGQRTLRALAQRLEALRNIDFFTDARAAAVAMALEDLRIECEARWSPG
                     EPVARVKGAIEALDAADYQGRTWATRQRPWVDRLASAWLVRRFIDPSARFVWLKPSAK
                     PPKSALGFDFDGARFTHVAGKVTFEVIAASFGLDDDAALRRLGEAVHYIDIGGAANEE
                     AAGLEALVRGLQTQHDDDDALLDAALGVFDALYSGLRGAA"
     gene            complement(1125..3101)
                     /locus_tag="VLN25_03310"
     CDS             complement(1125..3101)
                     /locus_tag="VLN25_03310"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HSQ09634.1"
                     /translation="MKPAEPLWRPSFERIERAHITAFLRWLEQERALTFTDYESLWRW
                     SVTDLEGFWGALWHFLDIGASRPYDKVLGARAMPGAEWFVGARLNLVDQVFRHVTDDR
                     PAILAGDERGEVRAVSWTDLRDQVASLAAALRGLGVVPGDRIVAILPNVPETIVAFLA
                     CASVGAIWSVCSPDIGPLAVLDRFRQIAPRVLIAGDGYRYGGKIFDRAGVLAEIRAAL
                     TSVEHTIIVPLLRDEVDTVRFPGALSWRALTGNAAELRTEQVASDHPLWVVYSSGTTG
                     LPKPIVHGHAGVLLEMLKGVHLHRDIGPDDRFFWYTTTGWIMWNVQVSGLLAGATVVL
                     YDGHPGTPDLGVLWRFVERVGATFFGAGAAYFANCIKAGIEPAQIADLSSLRTLGSTG
                     SPLPADSYHWVYEHVKRDLWLVSIAGGTDLAGALLTGQPTLPVYAGEIQCRALGLKVE
                     VWDDAGRPVIDEVGELVCSEPFPSMPLYFWNDAYGKRYRESYFERYPGVWRHGDWMRL
                     VPRPGAIGAIIYGRSDATINRHGIRMGTAELYRAVEALPEVLDSLVVDLEYLGRESFM
                     PLFVVLRADVALEESLRQRINARIGEALSARHVPSEIFAVTEVPRTLSGKKMELPIKR
                     LLLGYPLEQVAHVDAMANPASLAWFVEFARRRSG"
     gene            3148..3522
                     /locus_tag="VLN25_03315"
     CDS             3148..3522
                     /locus_tag="VLN25_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HSQ09635.1"
                     /translation="MNAFLHSGPPTEWTVICLCAEWCDTCREYRRLFDQRAARANDAL
                     HLWVDIEDDADVLGDLDIETFPTLLVLHGHRPRFFGPVLPQIEVIDGLLRTLDPLATA
                     IHIATDNETAVRWLCEQAQRMR"
     gene            3559..>4848
                     /locus_tag="VLN25_03320"
     CDS             3559..>4848
                     /locus_tag="VLN25_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSQ09636.1"
                     /translation="MTLLTMLDAELAFGLQPLLDRASLTVLEGDRIGLIGRNGTGKSS
                     LLNVVADRLDLDDGEVKRRDGLHVALVEQEPVLPVASTLRESLALRGALDGLHDERER
                     WRIEARLVEYLHRFGLDEALDPTRLSGGERKRGALALAFALTPDLMLLDEPTNHLDID
                     GITLLEDLLLKGPALIVITHDRAFLDRVASRIVELDRGLLRSYPGNYSAWEDRRATEL
                     AAEENASRKFDKFWAQEEAWIRKGIEARRTRNAGRVSRLESLRVERSARRERLGNVKL
                     TLDAGERSGKLVAELTDVCKRFGDKVVVDRLTTRIMRGDRLALIGPNGAGKTTLLRLI
                     LGTMAPDSGTVRRGTNLEIAYFDQMREALDPDRTVAETISPGSDWIEVAGSRKHVMSY
                     LGEFLFSPQRANSPIKTLSGGERNRLLLARLFARPANL"
BASE COUNT          851 a         1636 c         1600 g          761 t
ORIGIN      
        1 tcgctatctg cccggccggg ccgccgaagc taatgaagcc gagccgcagc cagtaggcga
       61 cggcctgcca gaagctgaca gcggccggcg cgcccgcggg gttcatgcag cacctcgcag
      121 tccgctgtac agcgcgtcga aaacgccgag cgcagcgtcg agcagtgcgt cgtcgtcgtc
      181 gtgctgtgtc tgcaggccgc ggaccagggc ttcgagtccg gcggcttcct cgttcgcggc
      241 gccaccgatg tcgatgtagt gcaccgcctc gccgagcctg cgcaatgcag cgtcgtcgtc
      301 cagcccgaag ctcgcggcga tgacttcaaa cgtgactttg ccggcaacgt gcgtgaagcg
      361 tgcgccatcg aagtcgaagc ccagtgccga tttcggcggc ttcgccgagg gtttcagcca
      421 gacgaaacgc gccgacgggt cgatgaagcg gcggaccagc cacgcactgg cgaggcgatc
      481 aacccagggg cgctgccgcg tggcccaggt gcgcccctgg tagtcggccg catcgagcgc
      541 ctcgatagcg cctttcaccc gcgcgaccgg ctcgcccggc gaccagcgcg cctcgcactc
      601 gattcggaga tcctccagcg ccatggcgac ggctgcagcg cgcgcgtcgg tgaaaaagtc
      661 gatattccgc agggcttcga gccgctgtgc gagcgcgcgc agcgtgcgct ggcccggaac
      721 ttcctcggcg gtccgcagtt ctcggcgcgc gcgggcgagg tcgcgctcga tggccttgta
      781 ctcgtccgag cggtcgaaca gggcttcgaa ttgccgttgc tgctcggcgt cgcgcgcgcc
      841 aaatgcgagc tgcgtagcct cgccatcggc ctggcgcact tcgtcggcga gtccgtcgaa
      901 cacctgcgcg cagtcgggcc gttcgggcag caggtaaacc ccgtcgcgca gcgcgccgca
      961 accgctcgcc ttcatcgcgc gccagatgcg cacccgcacc gcgctgggcg aggtcgggag
     1021 cgtgataacc agggcgagcc atccgagttg catgtagcaa ttattacata atgtaatagt
     1081 tattgcaagt cctttcttag cccgccgctg cgggccgccg gcgttcagcc ggagcgcctc
     1141 ctggcgaact cgacaaacca cgcgaggctc gcaggattgg ccatcgcgtc gacatgcgcg
     1201 acctgttcga gcgggtagcc gagcagcagg cgcttgatcg gcaattccat cttcttgccc
     1261 gagagcgtgc gcggcacttc ggtcaccgcg aagatctcgc tcggcacgtg gcgcgccgac
     1321 agcgcttcgc cgatgcgggc gttgatgcgc tgcctgaggc tctcttcgag cgccacatcc
     1381 gccctcagca cgacgaacaa cggcatgaag gactcgcgtc cgaggtactc gagatcgacg
     1441 accagcgagt cgagcacttc cggcagtgcc tcgaccgcgc ggtacagctc ggccgtgccc
     1501 atacggatgc cgtggcgatt gatggtcgcg tccgagcggc catagatgat tgcgccgatc
     1561 gccccgggac gcggcactag ccgcatccag tcaccgtggc gccagacgcc ggggtagcgc
     1621 tcgaagtaac tttcgcggta gcgctttccg taggcatcgt tccagaagta aagcggcatc
     1681 gacggaaacg gctcactgca gacgagttcg ccgacttcgt cgattaccgg ccggcccgcg
     1741 tcgtcccaca cctcgacctt cagcccgagc gcgcggcact gtatctcgcc ggcatacacc
     1801 ggcagcgttg gctgcccggt gagaagcgcg ccggcgagat cggtgccgcc ggcaatcgac
     1861 accagccaga gatcgcgctt cacgtgttcg taaacccagt ggtaggaatc cgccggcagc
     1921 ggcgaaccgg tcgagccgag cgtgcgcagc gacgagaggt cggcgatctg cgcgggttcg
     1981 atacctgctt tgatgcagtt ggcgaaatac gcggcgcccg cgccgaagaa agtcgcgccg
     2041 acgcgctcga cgaagcgcca cagcacgcca aggtccggtg tgccgggatg accgtcgtac
     2101 agcacgacgg tggccccggc cagcagcccg ctcacctgca cattccacat gatccagccc
     2161 gtggtcgtgt accagaagaa gcggtcgtcg ggcccgatat cgcggtgcag gtggacaccc
     2221 ttgagcattt cgagcagtac accggcgtgc ccgtgcacga tcggcttcgg cagtccggtc
     2281 gtgcccgacg aatacacgac ccagagcgga tgatccgacg ccacctgctc ggtgcgcagc
     2341 tcagcggcgt ttcccgtcag cgcgcgccac gacagcgcac cgggaaaacg cacggtgtcc
     2401 acttcgtcgc gcaagagcgg gacgatgatc gtgtgctcta cgctggtgag tgccgcacga
     2461 atctcggcga ggaccccggc gcgatcgaag atcttgccgc cgtagcgata gccgtcgccg
     2521 gcgatgagca cccggggcgc gatctgtcga aaccggtcga gcacagccag cggaccgatg
     2581 tcgggcgagc acaccgacca gatcgcaccg acgcttgcac aggcgaggaa cgcgacgatg
     2641 gtttccggca cattgggcag gatggcgacg atgcggtcgc cgggtacaac gccgaggccg
     2701 cgcagcgccg ccgccagcga cgcgacctgg tcacgcaaat cagtccacga gaccgcacgc
     2761 acctcgccgc gctcgtcgcc agcgagaatt gccggacgat catcagtcac atgccggaac
     2821 acctggtcga cgagattcag tcgcgctccg acgaaccact cggcccccgg catggcgcgc
     2881 gcgccgagca ccttgtcgta cggccgcgac gcaccgatat cgaggaagtg ccagagcgcg
     2941 ccccagaatc cttcaaggtc cgtcactgac caccgccaca gcgattcgta atcagtaaac
     3001 gtcagcgcac gctcttgctc gagccagcgc agaaacgcgg tgatgtgcgc gcgctcgatc
     3061 cgttcgaacg acggacgcca aagcggctcg gcgggtttca tgcggccgca gtctacgagg
     3121 ccgccacgcc acaggcacaa tggatcgatg aacgctttcc tgcactccgg accgccgacc
     3181 gagtggacgg tcatctgcct ttgcgcggag tggtgcgata cctgccgcga gtatcggcgg
     3241 ctcttcgatc agcgtgcggc gcgggcgaat gacgccctgc acctgtgggt cgacatcgag
     3301 gacgatgccg atgtcctcgg cgatctcgat atcgagacct ttccgacgct gctggtcctg
     3361 cacggtcatc ggccgcgctt cttcggaccg gtcctgccgc agatcgaagt gatcgacggc
     3421 ctgctgcgca cgctggaccc cctggcgacg gccatacaca tcgctacgga caacgagaca
     3481 gcggtgcgct ggctgtgcga gcaggcgcag cggatgcgtt gagccgctgc gacggcacct
     3541 gcttgcgaga atgcggccat gaccctgctg accatgctcg acgccgaact cgctttcggc
     3601 ctgcaaccct tgctcgaccg tgcgtccctg accgtgctcg agggcgatcg tatcggactg
     3661 attggccgca acggcactgg caagagttcc ctgctgaacg tggtcgccga tcgactcgat
     3721 ctcgatgacg gggaggtcaa gcgcagggac ggcctgcacg tggctttggt cgaacaggag
     3781 ccggtgctgc cggtggcctc gacgctgcgc gaaagcctgg cgctgcgcgg agcgctggat
     3841 ggactgcacg acgagcgcga gcgctggcgc atcgaggcgc gcctggtcga atacctgcat
     3901 cgctttgggc tcgacgaggc gctggacccg acgcgcctgt ccggcggcga gcgcaagcgc
     3961 ggcgcactcg cgcttgcgtt cgcgttgacg cccgacctga tgctgctcga cgaacctacc
     4021 aaccacctcg acatcgacgg catcacgctg ctcgaggacc tgctcctcaa gggaccggca
     4081 ctgatcgtga tcacgcacga tcgcgctttc ctcgatcgcg tggccagtcg catcgtcgag
     4141 ctggaccggg ggctgctgcg ctcgtatccc ggcaactaca gcgcgtggga agacaggcgc
     4201 gccaccgaac tggccgccga ggagaacgcc tcacgcaagt tcgacaagtt ctgggcgcag
     4261 gaagaggcgt ggatacgaaa gggtatcgaa gcgcggcgca cgcgcaacgc cggccgtgtg
     4321 tcaaggctcg agtccttgcg cgtcgagcgc agtgcgagac gcgagcgcct cggcaacgtg
     4381 aagctcacgc tggatgccgg cgagcgctcg ggcaagctcg tggccgaact gacggacgtc
     4441 tgcaagcgtt tcggcgacaa ggtggtcgtc gaccgcctga cgacgcggat catgcgcggc
     4501 gatcgtctcg cgctgatcgg gcccaatggt gccggcaaga ccacgctgct gcgcctgatc
     4561 ctcggcacga tggcaccgga cagcgggacc gttcggcgtg ggacgaacct cgagatcgcc
     4621 tacttcgacc agatgcggga ggcactcgat cccgacagga ccgtcgccga aacgatcagc
     4681 ccgggctccg attggatcga agtggcaggt tcgcgcaagc acgtgatgag ctatctcggc
     4741 gagttcctgt tctcgcccca gcgcgcgaat tcacccatca agacgctgtc agggggcgag
     4801 cgcaaccgcc tgttgctggc gcgcctcttc gcgcgaccgg ccaacctg
//
LOCUS       DATIRT010000138         2874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1393736, whole genome shotgun sequence.
ACCESSION   DATIRT010000138 DATIRT010000000
VERSION     DATIRT010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393736"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="VLN25_03325"
     CDS             complement(<1..968)
                     /locus_tag="VLN25_03325"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HSQ09637.1"
                     /translation="MSIGLLHSSIRSLPLVYRGKVRDSYAVGDDKLLLVTSDRLSAFD
                     VVMNQAIPDKGRVLNAMSNFWFEKLAHIVPNHLTGMAPEGVVAADERDQVRGRAVVAL
                     RLKQIPVEAVVRGYLIGSGWKDYQKSGEVCGIELPRGLQMASRLPEPIFTPATKAELG
                     EHDENISFNAAKAIIGAELAFKVQTVSLTLYRAAAEYALTRGIIIADTKFEFGTDADG
                     QLRLMDEVLTADSSRFWPADEYEEGKSPPSFDKQFVRDWLEQQHWNKAPPPPDLPADI
                     VEKTAAKYREALKRLTGKTRDWLEQQHWNKAPPPPDLPADIVEKTAA"
     gene            complement(1036..1458)
                     /locus_tag="VLN25_03330"
     CDS             complement(1036..1458)
                     /locus_tag="VLN25_03330"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HSQ09638.1"
                     /translation="MSYESRRYEAGVDAADGRTFKGKRGLQRVLNATRYSLDGLGAAW
                     AHEDAFRQELLLAAVMIPVALVVPVSVIEKILLVGVVVLVLIVELLNTGIEAAVDRDS
                     FEINPLGKRAKDFGSAAVMLSLLLAGGTWIAILATRFL"
     gene            1544..>2874
                     /locus_tag="VLN25_03335"
     CDS             1544..>2874
                     /locus_tag="VLN25_03335"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HSQ09639.1"
                     /translation="MPAIDYSRFYRYDDLMVLLRAFVAEHPQYISLETIGTSHEGRAI
                     PLLAITNTATGKAIDKPAYWVDGNIHSVELTASSACLYFTDWLMKGKDTDPEIRRALD
                     TRTVYVCPRINPDGAEWAMADKPRYVRSSTRRYPFDEEPVEGLIAEDLTGDGRILQIR
                     IADPNGPWKKHPQEPRLLVRRDPLDPPGGEYYRLLPEGRIDDYDGTSIGIAGVTGNAQ
                     GLDLNRNFPSNWRQEFEQLGAGPYPTSEPEVRAVVDFFINHPNICGGTSIHTFSGVLL
                     RPFGHAPDDKLPAEDLWTYQAVGKKGEELTGYPAISIWHEFQYYPGEFISGAFDWIYE
                     HLGMYIWTIEIWNPKKEAGIDNKEWIHWFRDHPIEDDLKVFEWAQRIAPADSANPGYV
                     DWTPFDHPQLGKVEIGGWNRMAVFTNPPPALREKEIARFPKWLLWQTLISP"
BASE COUNT          529 a          940 c          899 g          506 t
ORIGIN      
        1 gctgccgtct tttcgacgat gtctgccggc aggtcgggcg gcggaggcgc cttgttccag
       61 tgctgttgtt cgagccagtc gcgcgtcttt ccggtcaggc gcttcaacgc ctcgcggtac
      121 ttcgctgccg tcttttcgac gatgtctgcc ggcaggtcgg gcggcggagg cgccttgttc
      181 cagtgctgtt gttcgagcca gtcgcgcacg aactgcttgt cgaacgacgg cggcgatttg
      241 ccctcctcgt actcatccgc cggccagaag cgcgatgagt ccgcggtcag cacttcatcc
      301 atcagccgca gctggccgtc ggcgtccgtg ccgaattcga acttggtgtc ggcgatgatg
      361 atgccgcgag tcagtgcgta ctcggccgcc gcacggtaca gcgtcagcga gaccgtctgc
      421 accttgaacg ccagttcggc gccgatgatc gccttggccg cgttgaagct gatgttctcg
      481 tcgtgttcgc cgagctcggc cttggtggcc ggcgtaaaga tcggctccgg cagccgactt
      541 gccatctgca ggccgcgggg caattcgata ccgcagactt ccccggactt ctggtagtcc
      601 ttccagcccg aaccgatcag gtagccgcgc acgaccgcct cgaccgggat ctgcttgagg
      661 cgcagggcca cgaccgcacg gccgcgcacc tggtcacgct cgtcggcggc gacgacgcct
      721 tccggcgcca tgccggtcag gtgattcggc acgatgtgcg cgagcttctc gaaccagaag
      781 ttcgacatcg cattgagcac gcggcccttg tccggtatcg cctggttcat cacgacgtcg
      841 aacgccgaca ggcgatccga cgtcaccagc agcaacttgt cgtcgcccac cgcgtagctg
      901 tcgcgcacct ttccgcgata caccagcggc agagagcgga tggacgaatg caggagcccg
      961 atgctcattt ggaaaaaccc tccctgccga agtgtaaggc gccgcgcgcg ccagccgacc
     1021 gcgcgaacgg gccggtcaga ggaagcgcgt ggccaggatg gcgatccacg tcccgcccgc
     1081 aagtaggagc gaaagcatga cagcggcact gccaaagtcc ttggcacgct tgccgagtgg
     1141 attgatctcg aaactgtcgc gatcgacggc ggcctcgatc ccggtgttca gcagttcgac
     1201 aatgagtaca aggaccacta cgccgaccag caggatcttc tcgatgacgc tgaccggaac
     1261 gacgagcgcg acaggaatca tcacggccgc cagcagcagc tcctggcgga atgcatcctc
     1321 gtgcgcccac gcagcaccca gtccgtcgag cgaatagcgc gtcgcgttca gcacgcgctg
     1381 caacccacgt ttgcccttga aggttcgacc gtcggcggcg tcgaccccag cttcgtagcg
     1441 gcgagactca tatgacatgg gcgcggatga tacgaccgcg atcggcgttg aaatatgaca
     1501 gccgcgtatc atcgcgcgac tcgtcgccct cttcgctcgc gccatgcccg ccatcgacta
     1561 ctcacgcttc taccgctacg acgacctgat ggtgctgctg cgcgctttcg tcgcggagca
     1621 tccacagtac atctcgctgg aaacgatcgg taccagccac gagggtcgtg cgatcccgct
     1681 gttggcgatc acgaatacgg caaccggcaa ggcgatcgac aagcccgcgt actgggtcga
     1741 cggcaatatt cactcggtcg aactgacggc gtcgagcgcg tgcctgtact tcaccgactg
     1801 gctgatgaag ggcaaggaca ccgacccgga gattcgccgc gcgctcgata cccgcacggt
     1861 ttacgtctgc ccgcgcatca atcccgacgg tgccgagtgg gcgatggccg acaagccgag
     1921 gtacgtgcga tcgtcgacgc gccgctatcc gttcgacgaa gagccggtcg aagggctgat
     1981 tgccgaagac ctcaccggcg atggccggat cctgcagata cgcatcgccg atccgaacgg
     2041 accgtggaaa aaacatccgc aggagccgcg tctgctggtg cggcgcgacc cgctcgatcc
     2101 gcccggtggc gagtattacc gcctgctgcc cgaaggtcgt atcgatgact acgacggcac
     2161 ctcgatcggc atcgccggcg tgaccggaaa tgcgcagggg ctcgatctca atcgcaactt
     2221 tccgtcgaac tggcgacagg agttcgagca gctcggcgcg ggcccgtacc ccacctcaga
     2281 gcccgaggtg cgcgcggtcg tcgacttctt catcaaccat cccaacatct gcggcggcac
     2341 cagtatccac acgttttcgg gcgtgctgct gcgcccgttc ggccacgcgc cggacgacaa
     2401 gctgccggcc gaggatctct ggacctacca ggcggtgggc aagaagggcg aagagctgac
     2461 cggctacccg gcgatctcga tctggcacga gttccagtat taccccggtg agttcatcag
     2521 cggcgcgttc gactggatct acgagcacct cggcatgtac atctggacga tcgagatctg
     2581 gaatccgaag aaggaagccg gcatcgacaa caaggagtgg atccactggt tccgcgacca
     2641 cccgatcgag gacgacctca aggtgttcga gtgggcgcag cgcatcgcgc cggcggattc
     2701 cgccaatccg ggctatgtcg actggacgcc gttcgatcat ccgcagctgg gcaaggtcga
     2761 aattggcggc tggaaccgca tggcggtgtt cacgaatcca ccgccggcgc tgcgcgagaa
     2821 agagatcgcg cggttcccga agtggctgct gtggcagacg ctgatttcgc cgaa
//
LOCUS       DATIRT010000139         4259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1884820, whole genome shotgun sequence.
ACCESSION   DATIRT010000139 DATIRT010000000
VERSION     DATIRT010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1884820"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1324)
                     /locus_tag="VLN25_03340"
     CDS             complement(<1..1324)
                     /locus_tag="VLN25_03340"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSQ09640.1"
                     /translation="MAIIRDRLPAFTSSVPVIVIGAGAAGALAALAARDAGAKVLVLD
                     RDVSPTGATALSSGMIPAAGTSAQEAREVADTPEQFGSDIQAKSNGRSAAHLVEAYTR
                     ESAGTLEWIERTCGVSFELVEGVPPGHGLARMHALADRGGTALLSVLYRALAAGGVRV
                     QSAARVTDLYVDADERVLGVRYQRGERESVDIGCSAVVVATNGFAGNMELVARHLPDV
                     RGLPFAGHDSSQGDALGWGNELGAAIEDIDGFLAHSSVVMPQRLQLPWALMTEGAIQV
                     NLQGERFGNEHEGYSESALFVLAQPEGIAFNIYDERIHEVGLAMPNYLEAVTKGLIKR
                     AATLRDLADTLGVDRNGLEQSVALVNALAFEDDMDEFGRSCHASQMILGPYYGVQVTG
                     ALLGTEGGLAIDASGQVLRPDGTTLPNLLAAGGAARGLSGDGGGGYLEG"
     gene            complement(1327..2649)
                     /locus_tag="VLN25_03345"
     CDS             complement(1327..2649)
                     /locus_tag="VLN25_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HSQ09641.1"
                     /translation="MKTPLNRLPATRLARMIAAHEVSCEAVTHSFSDALRDREQDIRA
                     FTWFDAARALETAREFDRVDWRGPLHGLPVAVKDNIDTADIASEYGSAIFLGHVPQSD
                     AACVAALRSSGAYVFGKTVTAELANFTPGPTRNPHGLGHTPGGSSSGSAAAVAANMVP
                     FALGTQTAGSVIRPASFCGVVGFVPTRGAVPRAGVKAVSDTLDVVGAFARSVEDVALV
                     AAVLALQPQWQHAPSSAQAPTIAWTATPWSSQLAPSMLAALERVARLLAGRGARVREI
                     AWPYEPRSDCPAFTRLAEAQRTVQLFETARALGPEAQYRRELLSSRLAALIDEGRGVG
                     APDYVAALQVGRDCAAGLDQLFGDADILLTPSAPGEAPQGLDATGDPQFNRPWHFLGG
                     PQVSVPVPHDIGHGESGLPLGLQVVARPGDDVRALAAAHWIESQLGRH"
     gene            complement(2861..3688)
                     /locus_tag="VLN25_03350"
     CDS             complement(2861..3688)
                     /locus_tag="VLN25_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSQ09642.1"
                     /translation="MNLKGKTIFISGASRGIGLAIVKRAAADGANVAIAAKTVEPNPR
                     LPGTIHSAAAEIEAAGGKALAIQCDIRDEEQVVAAAKQTAETFGGIDILVNNASAINL
                     TPTEATPMKRFDLMFGVNVRGTFLCTQACLPYLKKSGERKRNPHILTLSPPLNMEPRW
                     FAPHVAYTMAKYGMSMCVLGHAEEFRSYGIAVNALWPRTVIQTAALQMIPGIQPEHCR
                     TPEIMADAAYAVLTSDAATTTGNFFIDDEVLTKAGVTDFGRYSVVPDSKQLLPDLFL"
     gene            complement(3685..>4259)
                     /locus_tag="VLN25_03355"
     CDS             complement(3685..>4259)
                     /locus_tag="VLN25_03355"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HSQ09643.1"
                     /translation="GARTQREALAYPLAALLARRLGGVVRWQLVAESGLTSEGVLQRL
                     VGSRPQAADIAVIVVGVNDITKDVPLAFALRQRQHIADWLHVHAGVRHVVFPALPEME
                     LFPAVPKPLAWYAGQAARRNNRAQARWAARDGGLSHLVMDGVARPDLFCQDGFHPAPA
                     LYARVAERLTDHIVEVVQATTAPKLTTEKP"
BASE COUNT          712 a         1476 c         1399 g          672 t
ORIGIN      
        1 ttccttcgag gtagccgccg ccgccgtcac cggacagtcc gcgcgcggcg ccacccgcgg
       61 ccagcagatt gggcagcgtc gtcccgtccg gccgtagcac ctggccgctg gcgtcgattg
      121 ccagaccgcc ttcggtgccc agcagcgcgc cggtgacttg cacgccgtag tacggtccga
      181 gaatcatctg cgaggcatga cacgagcgcc cgaactcgtc catgtcgtct tcgaacgcca
      241 gtgcgttcac caaggcgacg ctctgctcga gaccgttgcg atccacgccg agcgtgtccg
      301 caagatcgcg cagcgtcgcg gcgcgcttga tcagcccctt ggtgacggct tccaggtaat
      361 tgggcatggc caggccgacc tcgtggatgc gctcatcgta gatgttgaac gcaatgccct
      421 cgggctgggc cagcacgaag agcgcgctct ccgaataacc ctcgtgttcg ttgccgaagc
      481 gctcgccctg gaggttgacc tggatcgccc cctcggtcat cagcgcccag ggcagctgca
      541 ggcgctgcgg catcaccacg gagctgtgcg cgagaaagcc gtcgatgtct tcgatcgcgg
      601 cgccgagttc attgccccag ccgagcgcat cgccctggct gctgtcatgg ccggcgaacg
      661 gcaggccccg cacatccggc agatgccgtg ccaccagttc catgttgccg gcgaagccgt
      721 tggtggcgac cacaactgcc gagcacccga tgtcgacact ctcgcgttcg ccgcgctggt
      781 accgcacgcc gagcacgcgc tcgtctgcgt cgacatacag atcggtgacc cgcgcagcgg
      841 actgcacacg aacacctccc gccgcaagcg cgcggtacag caccgacagc aaagccgtgc
      901 cgcctcgatc cgccagcgcg tgcatgcgag ccagtccgtg gccgggggga acaccctcga
      961 ccagttcgaa gctcacaccg caggtccgct cgatccactc cagcgtgcct gccgactcgc
     1021 gcgtgtaggc ctcgaccagg tgcgcggccg atcggccatt ggacttggcc tggatgtcgg
     1081 agccgaattg ctccggagtg tcagcgacct cgcgtgcctc ctgcgccgat gtgccggcgg
     1141 cgggaatcat ccccgaggaa agcgcggtag cgccggtagg cgagacatcg cgatcgagca
     1201 ccagcacctt ggcgccggca tcgcgcgccg cgagtgcggc cagcgccccc gccgcgccgg
     1261 caccgatgac gatcacaggc accgacgaag tgaatgcggg cagccggtca cggatgatcg
     1321 ccatgctcag tgccgaccca actgcgactc gatccagtgc gccgccgcca gcgcccgcac
     1381 atcatcgccc ggtcgcgcca ccacctgcag cccgagcggc aatccactct cgccgtgccc
     1441 gatgtcatgc ggcaccggaa cactgacctg cggaccgccg aggaagtgcc agggtcggtt
     1501 gaactgaggg tccccggtgg catcgagccc ctgcggcgcc tcgccgggcg cgctcggcgt
     1561 caggaggatg tccgcatcac cgaagagctg atcgagacct gcagcacagt cgcgtccgac
     1621 ctgaagggcc gcgacatagt ccggcgcacc gaccccccgg ccctcgtcga tcaatgcggc
     1681 gagccgactc gacagcagct cgcgccggta ctgagcctcg gggccgagtg cgcgcgccgt
     1741 ctcgaacagc tgcacggtcc gctgcgcttc ggccagtcga gtgaacgccg ggcagtcgct
     1801 gcggggttcg tagggccagg caatctcgcg tacacgcgcg ccgcgccccg cgagcaggcg
     1861 tgccacgcgc tcaagcgcgg cgagcatcga cggtgccagc tgggatgacc acggtgtagc
     1921 cgtccacgcg atggtcggcg cctgtgccga ggatggcgcg tgctgccact ggggctgcag
     1981 cgccaggacc gcggcgacca gcgcgacatc ttcgacgctt cgcgcaaacg cacccacgac
     2041 atcgagcgtg tccgatacgg ctttcacacc ggcgcggggg accgcgcccc gcgtcggcac
     2101 gaatccgacg acgccgcaga acgatgccgg gcgaatcacc gagccggccg tctgcgtgcc
     2161 cagcgcaaac ggcaccatgt ttgcggccac ggcagcagcc gaaccactgg acgagccacc
     2221 gggcgtatga ccgagcccgt gcgggttgcg cgtggggccc ggcgtgaagt tggcgagttc
     2281 cgcggtcact gtcttgccga acacgtacgc cccggaactg cgcagcgccg ccacgcacgc
     2341 cgcgtcggac tgcgggacat ggcctaggaa tatcgcagag ccgtactcgc ttgcgatgtc
     2401 ggcggtgtcg atgttgtctt tcacggccac gggcagtccg tgcagcggcc cacgccagtc
     2461 gacgcggtcg aattcgcgcg ccgtttcgag cgcgcgcgcc gcgtcgaacc aggtgaatgc
     2521 gcggatgtcc tgttcgcgat cgcgcagcgc gtcgctgaac gagtgcgtca ccgcctcgca
     2581 cgagacctca tgggcagcga tcatgcgtgc aagccgggtg gccggcaggc gattgagcgg
     2641 cgtcttcatc aaatcccccg tgggtcgaat ctaccgcagg acctgcggcc atccactgcg
     2701 agcagtcaac aaaaaagggc acccgcagac gcccttatgt ctccaactcg cgctcgaagt
     2761 tgaaatgaca ggcacggccc gcgctttttc gagggccgga tgtccgatga acacagagag
     2821 cccgttgaac aagagcccgc tgaacaagag cccgctgaac tcagagaaac agatcgggta
     2881 gcagttgctt gctgtccggc acgaccgaat agcgcccgaa gtccgtaacc cccgccttcg
     2941 tcagcacttc gtcgtcgatg aagaagttgc cggtggtcgt tgcggcatcg gacgtcagca
     3001 cggcataggc ggcatcggcc atgatctcgg gcgtgcggca gtgttcgggt tgtatgcctg
     3061 ggatcatttg cagcgccgcc gtctggatca ccgttcgggg ccacagagcg ttcacagcga
     3121 tgccgtagct gcgaaattcc tcggcatgtc cgagtacgca catgctcatg ccgtacttcg
     3181 ccatcgtgta ggccacatgg ggcgcgaacc agcgcggctc catgttcagc ggcggtgaca
     3241 acgtcaggat gtgcggattg cgcttgcgct cgcccgactt cttcaggtac ggcaggcatg
     3301 cctgcgtaca gagaaacgtt ccgcgcacgt tcacgccgaa catcaggtcg aagcgcttca
     3361 tcggcgtcgc ttcggtcggc gtgaggttga tcgcgctggc gttgttgacg aggatgtcga
     3421 tgcccccaaa ggtctccgcg gtctgcttcg ccgccgcaac cacctgctcc tcgtcgcgga
     3481 tgtcgcactg aatcgcgagc gccttgccgc cggcggcctc gatctccgcc gctgcgctgt
     3541 ggatcgttcc gggcagcctg gggttcggct ccacggtctt ggccgcgatc gcgacattgg
     3601 cgccatcagc ggccgcgcgc ttgacgatcg ccagaccgat gccgcgtgac gcaccggaaa
     3661 tgaatatggt cttgcccttg agattcatgg tttctccgtg gtgagcttgg gcgccgtggt
     3721 cgcttgcacg acctcgacga tgtgatcggt gaggcgttcg gccacgcgcg cataaagcgc
     3781 cggtgccgga tgaaagccgt cttggcagaa caggtcggga cgggcgacac catccatgac
     3841 caggtgcgag agcccgccgt cgcgcgccgc ccagcgcgcc tgtgcgcgat tgttgcgtcg
     3901 cgccgcctgg cccgcgtacc acgccagcgg cttcggcacg gccggaaaga gttccatttc
     3961 cggcagcgcc ggaaagacga cgtggcgcac gccggcgtgg acgtgaagcc agtccgcgat
     4021 gtgctgccgc tgtcgcagcg cgaaggccag cggcacatcc ttggtgatgt cgttgacacc
     4081 gaccacgatc accgcgatgt cagcggcctg cggccgggag cccaccaggc gctgcagcac
     4141 gccttcggac gtgagtcccg attcggccac gagttgccag cgtacgaccc cgcccaggcg
     4201 ccgggccaac agcgcggcca gcggataggc cagagcttcg cgctgggtgc gtgcgccca
//
LOCUS       DATIRT010000140         3680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_705627, whole genome shotgun sequence.
ACCESSION   DATIRT010000140 DATIRT010000000
VERSION     DATIRT010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705627"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..348
                     /locus_tag="VLN25_03360"
     CDS             148..348
                     /locus_tag="VLN25_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09644.1"
                     /translation="MVTKLDKDLKRELLIRDKPFTLTIGPERLKLTPKGYRNGLELRW
                     EDLVDGDAALAAALNASIAPVR"
     gene            345..2933
                     /gene="ligD"
                     /locus_tag="VLN25_03365"
     CDS             345..2933
                     /gene="ligD"
                     /locus_tag="VLN25_03365"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HSQ09645.1"
                     /translation="MSDKASARTRSSPLSHYEGKRDFRVTPDPATSPARHARKRSKSL
                     SFVIQKHWASHLHYDFRLEFGGVLMSWAVPKGPTYDPSKMQMAIQVEDHPLDYAAFEG
                     TIPRKQYGAGTVIVWDRGTWEPIGDAADGIDKGKLLFKLHGEKLAGLWELVRISKPGE
                     KQDRWMLFKKRDEWARPLSEYDVITALPDSVVSQPLGLVEEREPRIATRSTVPRGSKR
                     ETAFDLSAAVRSTLPTKLEPQLATTVAVPPAGDWIVETKFDGYRLLARIEAGKARLFT
                     RGGHDWTTKMSSLAAAIEAIGIQSGWLDGEIVVMSDAGLPDFNALQNAIDAATADAIE
                     YFVFDLPYHDGRDLRKVPLRHRREVLRRLVEPKGSDRVHLSQSFDAAPAQMLEAAREM
                     RLEGIMLKRPEAPYVSARTETWLKLKCSARQEFVICGFALRSGSANEAGSLMLGYYQD
                     SKLTYAGNVGTGWNAKAGRELFSRLAAIEITRPHFDSQTLKPGRWTRRGPGQERWVQP
                     TLVAAVSFAEWTPAGHIRHAVFQGLRADIPPTSVSREPATAIPSAAPSAMTKAQAHIK
                     VTHPERVIDPSTGLTKLALVRYYESIGDRMLPHLQARPVSLVRAPEGVGGEIFFQKHM
                     DKPISGLRELDPGLWPGHSALLGIDSVAALTAAAQMNTIEFHTWNSTTRSIDKPDRMV
                     FDLDPGHGVAWSRVQEAALLVRTILTELKLQSWLKTSGGKGLHVVVPLAARLDYDTVK
                     SFSQAIVQHLARTIPQRFVAKSGADNRVGRIFVDYLRNGRSQTTVAAFSARARPGLGV
                     SMPISWDQVSSLKSAAQWSIATAREYLSFETSDPWADYWKAKQTLTAAMKTLDFERPQ
                     STRRPK"
     gene            3027..>3680
                     /locus_tag="VLN25_03370"
     CDS             3027..>3680
                     /locus_tag="VLN25_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HSQ09646.1"
                     /translation="MAARSIASLSLSFGLVSIPVKLYSATESSASVRFNMLSKDGSRL
                     KQQYVSERDQKVVERAEIVKGYEFEKDRFVIFAPDELKALQESPSHTIDIVAFIPANA
                     IDPIFYDKSYFLAPDKRGAKPYNLLTEAMKQSGRCALAKWSWKAKQYVVQVRAADEGL
                     VLQQLLYADEVRSMKELGIEKVTVSDAELKLAMQLIDQISRDTYDPTMFEDEEKKRIL
                     "
BASE COUNT          732 a         1127 c         1170 g          651 t
ORIGIN      
        1 gcagctttcg attcagagcg aggttttgag aaaggggcgc cacgtgcgcc ccttttcttt
       61 ctcgtctatt gcaggtgtgc cctaaacgcg gtcatcagca ggaattgctt ttatgatgaa
      121 gcggtccgtg gctgccggag aacgtcaatg gtcaccaagc ttgacaagga tctgaagcgc
      181 gagctgctta tcagggacaa acctttcacg ctcacgatcg gtcccgagcg tttgaagttg
      241 acaccgaagg gctatcgcaa tggtctcgaa cttcggtggg aggatctcgt cgacggcgac
      301 gccgcgctcg cggctgcgct caacgcctca attgcacccg tgcggtgagc gacaaggctt
      361 cagcccgaac ccggagttcg ccgctttccc actacgaggg gaagcgtgac ttccgcgtca
      421 cgcccgatcc tgcgacgtcg cctgcaaggc atgcacgaaa acgaagcaaa tcgctgtcgt
      481 tcgtcattca aaagcattgg gctagccacc ttcactacga cttccgactc gagttcggtg
      541 gcgtgctgat gagttgggca gtgcccaagg gtcccaccta cgacccttcg aagatgcaga
      601 tggccatcca ggtcgaggac catccgctcg actatgcagc cttcgaagga acgatcccgc
      661 gcaaacagta cggcgcgggg accgtcatcg tgtgggaccg cggtacgtgg gagccgatcg
      721 gtgacgctgc cgacggcatc gacaagggca agctgctgtt caaactccat ggcgagaagc
      781 tcgccgggct gtgggagctt gtgcggatct ccaaacccgg cgaaaagcag gatcgctgga
      841 tgctgttcaa gaagcgcgac gagtgggcac ggccgctctc cgagtacgac gtcatcactg
      901 cgttgccgga cagcgtggtc agtcagccgc tcggactggt ggaggagcgt gaaccgcgga
      961 tcgcaacccg cagcacagtg cctcggggca gcaagagaga gactgcgttc gacctgagcg
     1021 cggcggtgag gtcgacgcta cccacgaagc tcgaaccgca gctggccacg acggtggcag
     1081 tgccgccggc cggcgactgg atcgtcgaga ccaagttcga tggctaccgc ctcctggcac
     1141 ggatcgaagc cggcaaggcc cggcttttca cgcgcggagg tcacgactgg acaacgaaga
     1201 tgagctcgct cgcagctgcc atcgaggcaa tcggaatcca atcgggctgg ctcgacggtg
     1261 agatcgtcgt gatgagcgat gcaggcctgc ccgactttaa cgcgctgcag aacgccattg
     1321 acgccgcaac cgctgacgcg atcgagtact tcgtcttcga tctgccttac cacgatggcc
     1381 gcgatctgcg gaaagtgccg ctgcgccatc gtcgcgaagt gctgcgccgg ctggtcgagc
     1441 cgaaggggag cgaccgggtg catctgagcc aaagcttcga cgccgcaccg gcgcaaatgc
     1501 tcgaggccgc gcgcgagatg cgtcttgaag ggatcatgtt gaagcggccg gaagccccgt
     1561 atgtgtcggc acgcaccgag acctggctca aactcaagtg cagtgcacgc caggagtttg
     1621 tgatttgcgg attcgcgctg cgcagcgggt cggcgaacga ggccggcagc ctcatgctcg
     1681 gctactacca ggattcgaaa ctgacctacg ccggcaatgt cggtacgggc tggaacgcca
     1741 aggcggggag ggagctcttc tcgcgccttg ccgcgatcga gatcacgcgc ccgcatttcg
     1801 actcgcagac gctcaaaccg gggcgctgga cccgtcgcgg gcccgggcag gagcgctggg
     1861 tccagccgac gctggtcgcc gcggtgtcat tcgccgagtg gacgccggcc gggcacatcc
     1921 ggcacgccgt gtttcagggt cttcgagcgg acatcccgcc cacctcagtg tcgcgcgagc
     1981 ctgcgactgc cattccttcg gctgctccgt ccgccatgac aaaagcgcaa gcgcacatca
     2041 aggtcaccca cccggaacgg gtgatcgacc cgtcaaccgg cctcaccaag ctggcgctgg
     2101 tccggtatta cgagagcatc ggtgaccgga tgctgccgca cctgcaggcg cgcccggtat
     2161 cgctcgtgcg cgctccggag ggagtgggcg gcgagatctt tttccagaag cacatggaca
     2221 agccgatttc cggattgcgc gaacttgatc cggggttatg gccgggtcac tcggcgctgc
     2281 tcggcatcga cagcgtcgct gcactgaccg cggcggcaca gatgaacacg atcgagtttc
     2341 atacctggaa ctcaacgacg cgcagcatcg ataagccgga tcgcatggtt ttcgacctgg
     2401 acccgggcca cggggtcgcg tggtcgcgtg tgcaggaagc ggcactactc gtacgcacca
     2461 tcctgaccga actgaagttg caaagctggc tcaagaccag cggcggcaag ggcttgcacg
     2521 tcgtggtgcc gctcgcagcg aggctcgact acgacaccgt caagtcattc tcgcaggcga
     2581 tagtgcagca tttggcgcgc acgatcccgc agcgcttcgt cgcgaagagc ggcgcggaca
     2641 atcgggtcgg acggatcttc gtggactatt tgcgcaacgg ccggtcgcag acgacggtgg
     2701 ctgcgttttc ggcacgcgcg cggccgggtc tgggcgtttc catgccaatt tcatgggatc
     2761 aggtgtcttc gctcaagagc gccgcgcagt ggagcatcgc gaccgcgcgc gagtatctga
     2821 gtttcgagac atcggaccca tgggccgact actggaaggc gaagcagaca ctgaccgcgg
     2881 cgatgaagac gctcgacttc gaacgcccgc aatcaacgcg acggcccaag tgacaaaccg
     2941 gctcgcttcc ccttatgcta agtgcccgcc gcgcactagc cgtttccaag gccgttgaac
     3001 ggttcggcga ctcacaagga gaacccatgg ctgcgcgctc gatcgcatcg ttatcgctgt
     3061 ccttcggact ggtttcgatc cccgtcaagt tgtactcagc gaccgaaagc tccgcctcgg
     3121 tgcgtttcaa catgctgtcc aaggacggct cacgtctcaa gcagcagtac gtctccgaac
     3181 gggatcagaa ggtcgttgag cgcgccgaga tcgtcaaggg ctatgaattc gagaaggatc
     3241 gcttcgtcat cttcgcgcca gacgagttga aggcgctgca ggaaagtccc agccacacga
     3301 tcgatatcgt ggcgttcatt ccggccaacg cgatcgaccc gattttctac gacaagtctt
     3361 acttcctcgc gccggacaag cgcggcgcga agccctacaa cctgttgacg gaagcgatga
     3421 agcagagcgg acggtgtgcg ctcgcgaagt ggtcgtggaa ggcgaagcag tacgtggtcc
     3481 aggtgcgcgc ggctgacgag gggctggtgt tgcagcagct tctctacgcc gacgaagtcc
     3541 gctccatgaa ggagcttggc atcgagaagg tcaccgtatc cgacgccgag ctaaagctcg
     3601 ccatgcagct catcgaccag atctcgcggg acacctacga cccgacgatg ttcgaggacg
     3661 aagagaagaa gcgcattctc
//
LOCUS       DATIRT010000141         3743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1492090, whole genome shotgun sequence.
ACCESSION   DATIRT010000141 DATIRT010000000
VERSION     DATIRT010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492090"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="VLN25_03375"
     CDS             complement(<1..1184)
                     /locus_tag="VLN25_03375"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HSQ09647.1"
                     /translation="MSPLGITLLMLVGFAAFAWLAWRKLSIVVALAPENRFDQPWQRV
                     KRLLQVGFGQSRLLTGDFRPGLMHAVIFFGFLTLLVRKLHLIVIGYTPLATIPGTAGG
                     IYTAFKDLIELAVLVAVGYGYYRRMVRPPARLEPNREAILILTLIALIMITDFGFDAF
                     RFAQHAQTVPAIAHEAAWAWIGAPLSTLLSGLSPSTLDLGAHAFYWVQMATVFTFLAI
                     LPIGEHFHIVTALPTVYFGRDVPANRVTSVDLAPLAADDVDPDTLKIGAKTALDLTWK
                     EGLDAFTCTECGRCKDACPTYLTGKPLALKWVHDAVKHHLVEHAEAIRAHDAAKLPAI
                     IGSAISDVTLWAFTTCGYCEVACPIQLEHLPKFYRLRQRQVMMEGEFPHELNRVFAAY
                     ES"
     gene            complement(1334..1990)
                     /locus_tag="VLN25_03380"
     CDS             complement(1334..1990)
                     /locus_tag="VLN25_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09648.1"
                     /translation="MVGLLSSCAMYSSPPVARPSGSELPDPTGKMAVAAWQARLGQHI
                     ESAGGGDPAVLAELPALRSTAVARPSQIVFAATDIEALVPERDGFDVSGLLIGKHSTA
                     IGPRYMFIVGVVQRRDYWPVAVGDVRVAAMTVNGGVASWETGPADAEALMRYRAGTDQ
                     STTIRFPSVQDRFRVVDCDPGVCVEELRTGARWALYPGARAAPAPSAQRPVTPPQTAH
                     "
     gene            complement(2084..>3743)
                     /locus_tag="VLN25_03385"
     CDS             complement(2084..>3743)
                     /locus_tag="VLN25_03385"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HSQ09649.1"
                     /translation="AIESGQSQARIEELQSAEHIAGMVWSHIFAAIPANELLDATLVS
                     EAVQSQYPGLITMYRPPVSIYDDPLLLIDLTKAVRTFFRAGTVSAGEHVFGTDKIIHF
                     VNVGRIYHAKYEARLKRGLPEKEAIKSAIASTSRNFLLSESGVLGVFTTGILSNGDLA
                     ANYAGLMFYRNLTETVHIGPRTLPPMLERDGPLWRVQISPGSDVFVAFITPHWNEVLN
                     PNKYVRYTSGRISTLVRERCPQVLDWYRDPRGQTRDRAQFETIERELATYYGDDYGHQ
                     SNITNPVTVADLCFPGSADKSVGMRDDRVPSAPSRDSAVTASARGPDTLGRTPLWWAA
                     REGDVKKIRLLTEGSRTLDLADVDGETPLHAATRSGNTAAVHELASRGADLDRASVYG
                     VTPLMLAAASGRTELAVALLEAGASPNARDLFGKTALHDAALHGNRLLTNRLLESGAD
                     PRIANDGGTTPLQLAGRRGHETIVTLLVAHGADATARNDASASARDEAQKQEHVSTVD
                     RRLMDAHTTARDAHTTVGLRAPQQPGEPVVARSVDASRSPDRNP"
BASE COUNT          638 a         1162 c         1270 g          673 t
ORIGIN      
        1 tgcgactcat aggcggcgaa gacgcgattc agttcatgcg ggaactcgcc ctccatcatg
       61 acttggcgct gccgcagccg gtagaacttc ggcaagtgtt cgagttggat cgggcaggcc
      121 acttcgcagt agccgcaggt cgtgaatgcc cacagggtga cgtcgctgat cgcgctgccg
      181 atgatcgccg gcagcttcgc cgcatcgtgg gcgcggatcg cctccgcatg ctcgacgaga
      241 tgatgcttga ccgcgtcgtg cacccacttc agcgccagcg gcttgccggt cagatacgtg
      301 gggcaggcgt ccttgcagcg gccgcactcg gtgcaggtga aggcatcgag tccttccttc
      361 cacgtgagat cgagcgcggt cttcgcgccg atcttcagcg tgtcggggtc gacgtcgtcg
      421 gccgccagcg gcgcgagatc gacactcgtc acacggttgg cgggcacgtc gcgaccgaag
      481 tagaccgttg gcaatgcggt gacgatgtga aagtgctcgc ctatcggcag aatcgccagg
      541 aaagtgaaga ccgttgccat ctgcacccag tagaacgcgt gggcgcccag gtcgagcgtg
      601 gacggtgaca gaccggaaag cagggtcgac agcggcgcgc cgatccatgc ccaggccgct
      661 tcgtgtgcaa ttgcggggac ggtctgcgca tgctgtgcga atcggaacgc gtcgaagcca
      721 aagtcggtga tcatgatcag cgcgatcagc gtgaggatca ggatcgcctc gcgattcggt
      781 tcgagccgtg ccggcggacg caccatgcgc ctgtagtagc cgtagccaac ggcgacgagt
      841 acggcgagtt cgatcaggtc cttgaacgcg gtgtaaatgc cgcccgctgt gccgggaatc
      901 gttgccagcg gggtgtagcc gatcacgatc aggtgcagtt tgcgcaccag aagggtcagg
      961 aagccgaaga agatcaccgc atgcatgagc ccgggacgga agtcaccggt cagcagccgt
     1021 gactggccga aaccgacctg cagcagccgt ttcacgcgtt gccagggctg gtcgaagcgg
     1081 ttttcgggcg caagggcgac gacgatcgac agcttgcgcc aggcgagcca ggcgaatgcg
     1141 gcgaaaccca ccagcatcag cagcgtgatt ccgagcgggc tcatcgatgt cggttcatcg
     1201 gggtcatttc atcgaggtcg gaccgggcgg cccattgtcc aagtccggcc cggcggcagg
     1261 ctgacatcga tcaaccgcag gcgcgaccta ccgctggaag cggcgcagcc ggaggcaacc
     1321 ccggcttcag tgctcagtgc gcagtctgtg gtggtgtaac cggccgttgc gcggacggcg
     1381 ccggtgcggc ccttgcgcct ggatacagtg cccagcgcgc gccggtgcgg agctcttcca
     1441 cgcatacgcc gggatcgcag tcgacgaccc ggaaccgatc ctgcactgaa gggaatcgga
     1501 ttgtggtcga ctgatcggtg ccggctcgat agcgcatcag cgcctcggca tcggcgggac
     1561 ccgtttccca ggaagcgacg ccgccattga ccgtcattgc ggccacgcgc acgtcgccga
     1621 ccgccacggg ccaatagtcc cgacgttgga ccacgccgac gatgaacatg tagcgtggac
     1681 cgatagccgt gctgtgcttg ccgatcagaa gcccggacac gtcgaaaccg tctcgttccg
     1741 ggacaagtgc ctcgatgtcg gtcgccgcaa agacgatttg gctgggtcgc gccacggccg
     1801 tcgatcgcag cgccggcagt tctgccaata ccgccggatc gccgccgccg gcactttcga
     1861 tgtgctgccc aagccgagcc tgccaggccg caacggccat tttgcccgtc gggtccggca
     1921 attcgctacc cgacggtcgg gcgaccgggg gtgaggagta catggcgcag ctgctcagca
     1981 gacccaccat caacatcatg gtcgcgtgcg cgcatgcgac gcgcgtcgcg aagcgcgctg
     2041 cagttggcga aggggcatga ggtggcatat tgaaggtgcg ggctcagggg ttccggtccg
     2101 gcgatcgact cgcgtcgacc gagcgcgcca cgacgggctc ccccggctgt tgcggtgccc
     2161 gaagtccgac cgtggtgtgt gcgtccctcg cagtggtatg tgcgtccatc aacctgcgat
     2221 cgactgtgct cacgtgttcc tgtttttgtg cctcgtcgcg cgccgatgcg ctggcgtcgt
     2281 ttcgggccgt tgcgtcggcg ccgtgcgcca ccagcagcgt cacgatggtc tcgtggcccc
     2341 ggcggcccgc cagttgcagc ggagtagtgc cgccgtcgtt ggcgattcgc gggtcggcgc
     2401 cggattccag caggcggttc gtcagcaggc ggttgccatg cagggctgca tcgtgcaggg
     2461 ccgtcttgcc gaacaggtca cgcgcattcg gacttgcacc tgcctcaagc aatgcaacgg
     2521 cgagctcggt cctgccactg gcggccgcca gcatcagcgg cgtcacaccg taaaccgacg
     2581 ctcggtcgag gtcggcgcct cggctcgcca attcgtgcac ggctgccgtg ttgccggacc
     2641 tcgtggccgc atgtagcggc gtttcgccat cgacatcggc gaggtcgagt gtccgggagc
     2701 cctcggtcag gagtcggatc ttcttgacgt cgccttcccg ggccgcccac cacaagggtg
     2761 tacggcccag ggtgtccggg ccccgcgcgg aagccgtgac tgcgctgtca cgtgacggtg
     2821 ccgacggcac ccggtcgtcg cgcatgccga ccgacttgtc ggcgcttccg ggaaagcaca
     2881 ggtcggcaac ggtcaccgga ttggtgatgt tgctctggtg cccgtagtcg tcgccgtagt
     2941 aagtggcgag ctcgcgctcg atggtttcga actgtgcacg gtcacgggtc tgtcctcgcg
     3001 ggtcgcggta ccaatccagc acctgcggac agcgttcgcg gacaagggtg ctgatccgac
     3061 cgctggtgta gcgcacgtac ttgttcgggt tgagtacctc gttccagtgg ggcgtgatga
     3121 aggcgacgaa gacatcggag cctggcgaaa tctgaacacg ccagagcggg ccgtcacgct
     3181 ccagcatcgg cggcagggtt cgcggtccga tgtgcactgt ctcagtcagg ttgcgataga
     3241 acatcaggcc ggcatagttg gcagcgaggt cgccgttgga gaggattccg gtcgtgaaaa
     3301 cgccgagcac gccgctctcg gataacagga aattgcgcga tgtcgacgcg atcgcagatt
     3361 tgatggcttc cttctcgggc agtccccgct tgagccgggc ttcgtatttg gcgtggtaga
     3421 tccgaccgac gttaacgaag tggatgatct tgtcggtgcc gaaaacgtgc tcgcccgcgc
     3481 tgactgttcc ggcccgaaag aaggtccgaa cggccttggt caggtctatc agcagcagag
     3541 gatcgtcata gatcgatacc ggcggccgat acatcgtgat caggcccgga tactgagatt
     3601 gcaccgcttc ggataccagc gtggcgtcga gcagttcatt ggcagggatg gccgcgaaga
     3661 tgtgcgacca caccatgccc gcgatgtgtt ctgccgactg cagctcttcg atccgggcct
     3721 gcgactgtcc ggactcgatc gcc
//
LOCUS       DATIRT010000142        17560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_115272, whole genome shotgun sequence.
ACCESSION   DATIRT010000142 DATIRT010000000
VERSION     DATIRT010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 17560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17560
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115272"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VLN25_03390"
     CDS             complement(<1..643)
                     /locus_tag="VLN25_03390"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HSQ09650.1"
                     /translation="MTLLIDIGNTALKWAFTDARSEPAEVHVELHHDADNLAVKLSSA
                     WTSMPPGLAAVGCSVAASPVRAAVERAAKERGATVQWLGAEPRHDGAVVLVNGYRNPA
                     QLGADRWHGMLGACLRCPGQSFVLVAAGTATTVDCVQRGPDMGRFIGGCIAPGARMMF
                     EALARRTAGLPQARGAATDFPDNTDDAITTGVVDAQAGLAGQIVQRFARRLGHA"
     gene            complement(627..1508)
                     /locus_tag="VLN25_03395"
     CDS             complement(627..1508)
                     /locus_tag="VLN25_03395"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HSQ09651.1"
                     /translation="MTRLVSVSAAAAQAGRPEAPPLVTAVIERWVADAALPETLRVEV
                     VASTGSTNEDLLVRARRRRPEGSLLLAADEQTAGRGRQQRCWVARPRTALLFSLSVPL
                     AGLPAALPAVTLACGVALADYLIGRAVPVRLKWPNDLLLDGRKLAGVLCELAVDADGQ
                     ATLVVGVGINGCLGDADRAAIGQPAAALEERVPWALLAAEREAWIAALAGVVLLAVRR
                     FVGEGFSPWRMRFNELLDARGQRVDIVDDGHIVASGRVVEVDTIGRLMLATAGGSRSI
                     NVGDVSLRAAPSDDAAD"
     gene            1678..2790
                     /locus_tag="VLN25_03400"
     CDS             1678..2790
                     /locus_tag="VLN25_03400"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HSQ09652.1"
                     /translation="MERSSLIPTLRTQASPIARAACAVYGMLLLYSGLAPWAGWRDLG
                     IHALAFLTAPFPRYTTTFDLVVNFVAYLPLGALIVLALYPRVRGVSAIGLGVLGGALL
                     AGMVEAMQTYLPTRVASNVDLMSNSLGALAGATLTAPFAAALIDRGRLVEWRRRWFEH
                     GATAVLMAVALWPLAQIYPEPMLFGNGGLRGMLEPAITALGGDWWQFGAGLFGPAEFV
                     LAEAFVVASAILAVGLALTSVVRTNAPRYRLLAALLAAALVAKSLSNAVQFGPERALA
                     WLTPGVFGGISLGVLSLAAATGGPRRWQGRFAGIAVLALLVAVNATPDNPYHLAQMQE
                     WRQGRLLNFNALAQWLSALWPLFLAAALLLQQTRRS"
     gene            2869..3177
                     /locus_tag="VLN25_03405"
     CDS             2869..3177
                     /locus_tag="VLN25_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HSQ09653.1"
                     /translation="MSHYKHHVFFCLNEREDGAACCSRHHATEMQQYAKARVKELGLS
                     GEGKVRVNKAGCLDRCDECPCIVVYPDAVWYTYVDRQDIDDIIDSHLIHGRVVDRLCL
                     "
     gene            3177..3857
                     /locus_tag="VLN25_03410"
     CDS             3177..3857
                     /locus_tag="VLN25_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSQ09654.1"
                     /translation="MTPRAEKRLIAGPAGRIEVALDWPLASPVAGVACIGHPHPLYGG
                     TMDNKVAATIARTFAALGWLAVRFNFRGVGSSDGTHDAGHGETEDFLHVLRTVPTLPG
                     VPERLPPTYPLALAGFSFGSFVAARAAEVLADGARPADYLVLVGAAAGKWPMPEVRTD
                     TLVIHGESDETIALPAVLDWARPRGLPVLVIPGADHFFHRKLPVLKARISAHIRGMSQ
                     FDAAEQSH"
     gene            3934..4446
                     /locus_tag="VLN25_03415"
     CDS             3934..4446
                     /locus_tag="VLN25_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09655.1"
                     /translation="MDGSLVYSKTPKGVAEIAARSAQLPMTTRRVLIMVDGKRTIDEL
                     AVLLRPGEIDTVMTQLESAGLIQRSSSSHSLDVPTVAGREMDAMPTTQGAPSSDDTNP
                     MTLEEAKRRAVRELTDRLGPDAEGLAIRIENCRTIEDFRERVREAERFVTAALGQAAA
                     ADYLRALRKR"
     gene            4532..5725
                     /locus_tag="VLN25_03420"
     CDS             4532..5725
                     /locus_tag="VLN25_03420"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HSQ09656.1"
                     /translation="MKPTFLISAHVTALVIALLCTCLAHAQGPALSARAWLLIDVASG
                     TTLAAHAPANRLEPASLVKLMTAYVVFIALAEKRIAIDDAVAVSRAAFVAPGRAGSRM
                     FIEPGRTVTVQELLRGMIVASGNDAAVALAEHASGSQAAFVERMNDEARRLGLVDTRF
                     TNPSGQSDPQQYSTARDLARLALRIGNDFPQHMALFAERDLTYNGITQANRNRLLWSD
                     PTVDGMKTGHLEASGWSIVATATRPRGKGDGAFERRLIAVVLGAPSDTVRAQDALRLL
                     NYGYAAYDTVRLYRGGAILVRAEVWKGDSAEVPLGPERDVFVTLPTDELQRLGEKGLR
                     SSIERPDPLIAPLTQGETVGRLKITANGQALTDAPIVALEGVAPAGLLGRAYDAVRLW
                     WRRRN"
     gene            5725..6645
                     /locus_tag="VLN25_03425"
     CDS             5725..6645
                     /locus_tag="VLN25_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HSQ09657.1"
                     /translation="MEWNESTCFINGDWRPLVDATVSVLDRGFIFGDGVYELVPVDTV
                     DGVRAPFRAHEHCERLQRSCDGIRLANPYSTDQWLDLIDAIVERHPWPRQAVYFQVTR
                     GVAKRDHAFPAGVAPTVFITTSPWPAVPAEQIERGIAVVTHADERWLHCDIKSISLLG
                     NVLMKQHAVEHGAFETVMLRDGFLTEASSANVMVVKKGTLLAPRPSAQILPGITCDAS
                     YALARQHDMPIELRPVSEAELRSADELWLSSSGREVLPITTLDGRPVGEGRPGPMYAR
                     MRQWFQAAKRAEAKHWQERKARQARGTLAA"
     gene            6720..7001
                     /locus_tag="VLN25_03430"
     CDS             6720..7001
                     /locus_tag="VLN25_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HSQ09658.1"
                     /translation="MNDAADRTEPKGLEFPTDFPIKIMGRSDDAFAQSIVKVVLEHAP
                     DFDASTLEMRASRDGNYISLTATIRAVSREQLDALYRALTSHPMVKIVL"
     gene            7060..7698
                     /gene="lipB"
                     /locus_tag="VLN25_03435"
     CDS             7060..7698
                     /gene="lipB"
                     /locus_tag="VLN25_03435"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HSQ09659.1"
                     /translation="MIVRDLGRSEYLPTWHAMRDFTAHRTESSDDELWLVEHPPVFTL
                     GQAGRREHLRSDTPIPVVPTDRGGQITYHGPGQVVAYPLVDLRRLGIFVKALVFRIEH
                     SVMQTLAMFDVDARRVPGAPGVFVPWGDGLTSGEFAGLAKIAALGIKVSHGRSYHGVA
                     LNVAMDLAPFSLINPCGYAGLPVIDLATLGVSARPEAVSARLAERLVAHLSA"
     gene            7799..8800
                     /gene="lipA"
                     /locus_tag="VLN25_03440"
     CDS             7799..8800
                     /gene="lipA"
                     /locus_tag="VLN25_03440"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HSQ09660.1"
                     /translation="MSEQSPIVRDPYAKLRGEGKTGKVFKKFDIKPVPANEALKKPEW
                     IRVKAGSPATRFYEIKAILREHKLHTVCEEATCPNIGECFGKGTATFMIMGDKCTRRC
                     PFCDVGHGRPDPLDADEPVNLARTIAALKLSYVVITSVDRDDLKDGGAQHFVDCIRAT
                     RELSSATRIEVLVPDFRGRLERALGILEAAPPDVMNHNLETVPRLYRESRPGSDYQHS
                     LTLLQEFKRRVPGVPTKSGLMVGLGETDDEILQVMRDLRTHGVDMLTIGQYLAPTSHH
                     LPVRRYVHPDVFRMFESEAAGMGFSHAACGAMVRSSYHADQQAHAAGLVDMSIAKGD"
     gene            9338..>17560
                     /locus_tag="VLN25_03445"
     CDS             9338..>17560
                     /locus_tag="VLN25_03445"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HSQ09661.1"
                     /translation="MLPSSFFRELPAVDSGPLIGLPRIYGIAWAFTAHTDSRFDSGLL
                     HRFVVAYQRVQPLTLAELWALPIMLRIVMIENLRRLAARIVESIDGRELADHFVDQLL
                     ALGPSGTSNEPDWPFSGDVPLPRASAAQLYQRLRYSDAVAAPILRTLTERLSKEGTHP
                     EEVVRLEVDRQTAADLSVRNLITSLRQISSFEWQKFVEAASQVHLALCAAPLYSSMDF
                     ITRDRYRHAVEELARRSPLTELEVAGVAVRRAAEADAATSARLLDPGYYLIDDGRVDI
                     ESEISYRVPLARQLFRAALRIALPIYVGAIFFLTFGIVAIAIAASADAGIEAAVLLLL
                     GALAIFPASDIAVTIVNRLVGIAVAPRHLPRLELAGGIPASMRTFVVVPTMLGSDESI
                     DEQVHQLETRHLSSGPGALYFALLTDWPDAEQEHMPDDRAQYDYAVAAIQRLNSRYPA
                     RADDSSDGYQRFYLFHRRRLWNASESRWMGWERKRGKLQEFNHLLRGANDTTYLTDEH
                     YVPPPDEVRFVLTLDADTILPIGAAAELVGTLVHPLNRPQLDASGRVVQGYGILQPRI
                     TPALPTRVGATRFQQLFSGNAGVDPYTFQVSDVNQDLFGEGTFTGKGLYDVDAFETSL
                     AGRTPENAILSHDLFEGSFARCGFVSDVELFEEFPSHTGVADMRAHRWVRGDWQLLPW
                     ISGRYWHTIPTLARWKMLENLRRSLSAPAMFLMLVASWLIPAIAPGPWIGLVLVTLLL
                     PQMLPLVERLVPRPNLDRSHWLRSLATDFVSGGTRAGVNLALLAHRAWLTSDAIVRTL
                     ARLAKSRNLLEWNTSAQVQSAARLSARSFVKSMLPSMVIVIAVGIAVFTTQPDHIWIA
                     LPLFALWLLAPFIARRISTPTVHGAAGSVAPADIVALRLIARRTWLYFATFVNKANHF
                     LPPDNFQEDPSPVIAHRSSPTNFGLYLLSVVAARDLGWISLHEMTSRIEATLDGMDAL
                     PRHRGHFLNWIDTLDLRPLEPRYVSTVDSGNLAAMLLVLSQACKEALDRPLAHQSALA
                     GIGDEVKLLRRSLARADESRGAAGVGFDQLHEALQRVEQLLEATPLAPRDRDQHMEAL
                     ESAVSDVHDIVSTLASEGNTGQLQEALLWAEAMHLNITAQRRERLELAPWLEAIAREQ
                     RATETTEGAPSTVMQPVAQEVLADIDRSRHAVEELADRLNTLSRRARLMFDEMDFRFL
                     YDETRDLFSIGYLVEDARLDLGCYDLLASEARLTSFITIAKRDVPATHWFRLGRTLVP
                     VDGGAALVSWSGSMFEYLMPSLLMDTPHGSLLEATVRRVIDRHINFGRERMVPWGISE
                     SAYNARDIHLTYQYSNFGVPGLGLKRGLSKDTVVAPYATALAAMYDAKAAVSNFEHLT
                     AVGALGRYGFYEALDFTPSRLPKSKEVAIVRAYMAHHQGMTLVALTNVVLKGEMRHRF
                     HRIPMVQATELLLQETAQRDVTLALPRSDRATPAIASSAAVPIDRRVNSPHAVPPATQ
                     LLSNGRYSVMVTAAGSGYSRWRELAVTRWREDATRDCWGTYLYLRDPASGYLWSAGYQ
                     PVCAEPVYYEAEFAEDRVRILRADAGIESSMEIMVSAEDDVEIRRLTLKNTGSRRREI
                     EITSYAEIVLASPAADEAHPAFSNLFVQTEYLSAVNGLIATRRPRSAEDEALWAAHVV
                     AYDSKDDSSSGIEFETDRVKFLGRGRSVRAPLSLMEGRRLSNTVGAVLDPVASLRIRL
                     AIAPGATVHASFATMTATSRESILNLADKYHDPGAFDRASTLAWTHAQVKLHYLGIDP
                     AEAQVFQQLASRVLYSDALLRAPRELLQGNRLPVSGLWKFGISGDRPILLARIEDGAD
                     IGVIRQLLTAHEYLHVKRLGIDLVILNEQATSYAEGLQQDLEMRVRESDARKGGDDPG
                     GRGTIYVLRADLLSEDERALLLTAARVVVVCGQGNLSAHLAMRHREPVLQVQPRVHAA
                     PSTSKLLHPPPLQFPNGLGGFTESGREYAVVLNRGQRTPLPWINIIANDGFGFHVSES
                     GAGYTWAGNSRENQLTPWSNDPVSDPVGEAFYLLDRDSGVLWTPTALPIRVEDSSYIT
                     RFGHGYARFQHRSNGIESELVQFVASEDPVKISRLRLINHSSQTRHISITAYVEWVLG
                     FSRTVNAPFIVTEADPETGGVFAFNPWHREFGNRIAFVAWDAPADGLTCDRQEFVGRN
                     GHLEAPVALTADVSLSGRWGAGFDPCVALQREVQIEPESQIELTFVLGQAADADAARA
                     LIRRFRTVKEADAELLHVKQQWREILDRVQVETPDPQMNLVLNHWLLYQTIVCRLYAR
                     SAFYQAGGAYGFRDQLQDVMALTITRPDLTRRQIVNASSHQFREGDVQHWWHPPFGRG
                     VRTHFSDDRVWLPYCVSHYLTTTGDTGVLDERVPFVEGDLLPPESEAMYFEPAISDEK
                     VTVYEHCARALDVSMAIGQHGLPLMGGGDWNDGMNRVGAEGRGESIWLAWFLCAALRE
                     FAPVAAQRGELERARAWSDHADRLQTAVEHEAWDGAWYRRAYFDDGTPLGASGNAECR
                     IDSIAQSWALISGAGEPARARRAMESVGEHLVKPGDSMVLLFAPPFDRSAQEPGYIKG
                     YLPGVRENGAQYTQAAVWCVIAHALQGDGERAAELFEMLNPINHSSTRAGIHRYKAEP
                     FVIAADVYSEPPHTGRGGWSWYTGAAGWMYRAGIEFILGFKLRGREILIDPCIPEQWR
                     SFKIRYRAPQSTYEFVIDNPQGVARGVLR"
BASE COUNT         3007 a         5755 c         5669 g         3129 t
ORIGIN      
        1 gcgcatgtcc cagtcggcgc gcaaagcgct gcacgatctg accggccagt ccggcctgtg
       61 cgtccacgac gcccgtcgtg atggcgtcgt cagtattgtc cgggaaatcc gtcgccgcgc
      121 cgcgggcctg cggcaatcct gccgtacggc gggccagtgc ctcgaacatc atccgtgccc
      181 ccggcgcaat gcagccaccg atgaagcgcc ccatgtcggg gccacgctgc acgcagtcga
      241 ccgtggtggc cgtccccgcc gcgaccagca cgaacgattg cccggggcag cgcaggcagg
      301 ctcccagcat gccgtgccag cgatcggccc cgagctgggc gggattgcga tagccgttga
      361 ccagcacgac tgcgccgtcg tggcggggct cggcaccgag ccattgcacc gtcgcgcctc
      421 gttccttggc tgcacgctcg accgccgcgc ggaccggcga cgcggccacg ctgcatccca
      481 ccgccgccag gcccgggggc atcgacgtcc acgccgaaga caatttcacc gcaaggttgt
      541 cggcatcgtg gtgcagttcg acgtgcacct cggcgggctc gctgcgggca tccgtgaacg
      601 cccatttcag cgccgtgttg ccgatgtcaa tcagcagcgt catcggacgg cgcagcgcgc
      661 agggaaacat cgccgacgtt gatactgcgg ctgccgcccg cagtggccag catcaggcgg
      721 ccgatcgtat cgacttcgac gacgcgacca ctggcgacga tgtgaccatc gtcgacaatg
      781 tcgacccgtt gaccacgggc gtccagcagt tcgttgaagc gcatgcgcca cggcgagaac
      841 ccctcgccca cgaaacgccg cacggcgagc agcacgacac cggcgagcgc ggcgatccag
      901 gcttcgcgct cggcggcaag cagcgcccat gggacgcgct cttccagggc cgcggccggt
      961 tggccaatgg cggcgcgatc cgcgtcgcca agacagccgt tgatgccaac gcctacgacc
     1021 agggtggcct gcccgtcggc gtcgacggcc agttcacaga gcactcccgc cagcttgcgt
     1081 ccatccagca gcagatcgtt tggccacttt agcctgacgg gtacggcgcg gccgatcagg
     1141 tagtcggcca acgccactcc gcatgcaagc gtcacggcgg gaagggctgc cggtagcccg
     1201 gcgagcggta ccgagaggga aaacagcagc gcggtgcgcg gccgggcgac ccagcagcgc
     1261 tgctgacgac cgcgtcctgc cgtctgttca tccgcggcga gcagcaacga accctcgggt
     1321 cggcggcgcc gggcgcgaac caggaggtct tcgttggtcg aacctgtact tgccacgact
     1381 tcgacccgca gggtctccgg caacgcagcg tcggcgaccc agcgttcgat gaccgcagtc
     1441 accagcggcg gcgcctcggg gcggccggcc tgtgcggcag ccgcggagac agatacgagg
     1501 cgggtcacgc gcaccgaccc gagtgccgac cggatgtcac cgactgtgtc actgtatgga
     1561 gcactgaatg tgcgaccgtg cgcgaccgac ttcaacgcga gtcactgtag catcggccag
     1621 ccgctgcggt gacccggggt ccatcatccc ttcagcaccg cgccaggcaa cgaaacgatg
     1681 gaacgctcca gcctgattcc gacactgcga acccaggcct cgccgatcgc acgcgcggcc
     1741 tgtgcggtct atggaatgct gctgctgtac tccggcctgg ccccgtgggc cggctggcgc
     1801 gatctcggca tccatgccct tgcattcctg acggcgccct ttccgcgcta cacgacgacc
     1861 tttgacctgg tcgtcaattt cgtcgcttac cttcccttgg gtgcactgat cgtgctcgca
     1921 ctctatccgc gcgtgcgcgg cgtgtcggcc atcggactgg gcgttctggg tggcgcactg
     1981 ctggcgggga tggtcgaagc gatgcagacg tatctgccaa cgcgtgtcgc gtcgaatgtc
     2041 gacctgatga gcaactcgct cggtgcgctg gcgggggcca cactgacagc gccgttcgcc
     2101 gcagcgttga tcgaccgcgg ccggctcgtc gagtggcgcc gccgctggtt cgagcacggc
     2161 gccaccgcgg tgctgatggc cgtcgcgctg tggccgcttg cgcagatcta ccccgaaccc
     2221 atgctgttcg gcaacggcgg cctgcgcggc atgctcgagc ccgcgatcac ggcgctcggc
     2281 ggggattggt ggcaattcgg cgccggactc ttcggccctg ccgagttcgt cctggccgaa
     2341 gccttcgtgg ttgcatcggc gatactcgcg gtcggcctgg cgctcacctc ggtagtgcgc
     2401 acgaatgccc cgcgatatcg attgctggcc gcgctgctgg cggcggcgct ggtggcaaag
     2461 tcgctttcca acgcggtcca gttcggcccg gaacgcgcgc tggcgtggtt gacgccggga
     2521 gttttcggcg gcatttcact cggcgtactt tcgttggccg ccgccacggg cggtccgcgt
     2581 cggtggcagg gtcgtttcgc cgggatcgcc gtccttgcac tgctggtggc cgtcaatgcc
     2641 acccccgaca atccctatca cctggcgcag atgcaggagt ggcgacaggg gcggctactc
     2701 aatttcaatg cgctcgcgca gtggctgtcg gccttatggc ccctgttcct cgcggcggct
     2761 ttgctgctgc agcagacgcg ccgcagttga attggccctt gaacctgtag ctgaacacgt
     2821 cgttggatcg ggatcgcttc ctataattga tcttttggca tcgaccccat gagtcactac
     2881 aagcatcacg tcttcttctg cctgaacgag cgtgaggacg gtgcggcctg ctgcagccgt
     2941 catcacgcga ccgagatgca gcagtacgcg aaggctcgcg tcaaggagct tggcctcagc
     3001 ggtgaaggca aggtccgcgt caacaaggcc ggctgcctgg accgctgcga cgagtgtccg
     3061 tgcatcgtcg tctatcccga cgccgtctgg tacacgtacg tcgatcgtca ggacatcgac
     3121 gacatcatcg actcgcacct gatccacggc cgggtggtcg accgactgtg cctctgatga
     3181 cgccgcgcgc cgagaagcga ctcatcgcgg gaccggcggg tcggatcgaa gttgcgctgg
     3241 actggccgct cgcatcgccg gtcgccggcg ttgcctgtat tggtcatccg cacccgctgt
     3301 acggcggaac gatggacaac aaggtcgcgg ccacgattgc gcgcacgttt gcggcactgg
     3361 gctggctcgc ggtgcgcttc aattttcgtg gggtcggttc atccgacggc acgcacgatg
     3421 ccggtcacgg ggaaaccgaa gacttcctgc acgtcctgcg caccgtgccg actctgccgg
     3481 gcgtcccgga gcgcctgccc ccgacctacc cattggcact cgccggattt tcctttggca
     3541 gcttcgtcgc cgcacgggcg gccgaggtac tggccgacgg cgcgcgaccg gccgactacc
     3601 tcgtcctggt gggcgcagcc gccggcaaat ggcccatgcc ggaggtgcgc acggacacgc
     3661 tcgtgatcca cggcgaaagc gacgagacaa tcgctctgcc ggccgtgctc gactgggccc
     3721 gaccgcgggg gttgccagta ctcgtgatcc cgggcgccga tcacttcttt catcggaaac
     3781 tcccggtgct caaagcgcgg ataagcgcac acattcgagg catgtctcaa tttgacgccg
     3841 ccgagcaaag ccattaaaag gtttgggctt ggtgcccgct gcagcgaagc cggcgccgaa
     3901 aaggagaatc gccgctgacg gcgactggga cacatggacg gctcactcgt ctactcgaag
     3961 accccgaaag gtgtcgcgga aatcgccgcc cgcagcgcac agctgccgat gacgacgcgc
     4021 cgcgtgctca tcatggtcga tggcaagcgc acgattgatg agctggcggt gctgctgcgc
     4081 cccggcgaaa tcgacacggt gatgacccag ctggaaagtg cagggctgat acagcgcagt
     4141 tcatccagcc attcgcttga tgtgccgacg gtggccggac gtgagatgga tgccatgccc
     4201 accacgcagg gtgccccgag cagcgacgat acgaacccga tgacgctcga ggaggccaag
     4261 cgccgcgcgg tgcgcgaact caccgaccgg ctcggaccgg acgccgaagg gctggcgatc
     4321 cgcatcgaga actgccgcac gatcgaggac tttcgcgagc gcgtgcgcga agcggagcgc
     4381 ttcgtgacgg cagcgctggg ccaggccgcc gccgccgact acctgagggc gctgcgcaag
     4441 cgctagtccg cagccgaccg accgggaact ctccggtcat cgcgctatcg tacggaactc
     4501 cgccacatgc gtggccactg cctttcctga agtgaaaccc acgttcctca tcagcgcaca
     4561 cgtcactgcg ctggtgatcg ccctgctttg cacctgcctt gcccacgcgc aggggccagc
     4621 gctgtccgcc cgcgcgtggc tgttgattga tgtcgccagc ggcacgacgc tcgccgccca
     4681 cgcgccggcg aatcggctcg agccggcttc gctggtgaag ctgatgacgg cgtatgtcgt
     4741 gttcatcgcg ctcgcagaaa agcgcattgc gatcgacgat gccgtggccg tctcgcgcgc
     4801 cgcatttgtc gctccgggac gcgccggctc gcgcatgttc atcgagcccg gccggaccgt
     4861 cacggtgcag gaactgctgc gcggaatgat cgtggcgtcg ggcaacgatg ctgccgtggc
     4921 gctcgccgag cacgcctccg gttcgcaggc cgcgttcgtc gagcgcatga acgacgaggc
     4981 ccggcggctc ggactcgtcg acacgaggtt caccaacccg tcgggacagt cggacccgca
     5041 gcagtactcg acggcgcgcg acctcgcacg cctggcgctg cgcatcggca atgactttcc
     5101 gcagcacatg gcgctgttcg ccgagcgtga tttgacctac aacggcatca ctcaggccaa
     5161 ccgcaatcga ctgctgtgga gcgacccgac ggtcgatggc atgaagaccg gtcacctcga
     5221 ggccagtggc tggtcgatcg tggccaccgc gacgcgaccg cgcgggaagg gcgacggggc
     5281 ctttgagcgg cgactgatcg ccgttgtgct gggagcgccc agcgacaccg tgcgtgcgca
     5341 ggacgcactg cgcctgctca actacgggta cgccgcgtac gacacggttc gcctctaccg
     5401 cggtggcgcg atcctggtcc gggccgaggt ctggaagggc gacagcgccg aagtaccgct
     5461 cgggccggag cgggacgtgt tcgtcaccct gccgacggac gaactgcagc ggctcggcga
     5521 gaagggtctg cgttcctcga tcgaacgtcc ggacccactg atcgccccgt tgacccaggg
     5581 cgaaaccgtg ggccgcctga agatcactgc gaatggacaa gcgctcacgg atgcgccgat
     5641 cgtcgctctc gagggcgtcg cgcctgcagg tctgctgggt cgcgcgtacg atgcagtcag
     5701 actgtggtgg cggaggcgca actgatggaa tggaacgaat cgacctgctt cattaacggc
     5761 gattggcggc cgctggtcga cgcaacggtt tccgtgctcg atcgcggttt catcttcggt
     5821 gacggggtgt atgagctggt tccagtcgat accgtcgacg gcgtgcgcgc gccgttccgt
     5881 gcacacgagc actgcgagcg actgcagcgc agctgcgacg gtatccggct cgccaacccg
     5941 tactcgaccg accagtggct cgacctgatc gacgcgatcg tggagcgaca cccctggccg
     6001 cggcaggccg tctacttcca ggtcacgcgc ggcgtggcca agcgcgacca cgcgttcccg
     6061 gccggtgtgg caccgacggt gttcatcacc acgtcgccgt ggccggccgt ccccgccgaa
     6121 cagatcgagc ggggcattgc ggtcgtcacg catgccgacg agcgctggct gcactgtgac
     6181 atcaagagca tttcgctgct cggcaacgtg ctgatgaagc agcacgccgt cgagcatggc
     6241 gccttcgaga ccgtgatgct gcgtgacggc tttctcacgg aagcctcgag tgccaacgtg
     6301 atggtggtga agaaaggaac gctgctggcc ccgcgtccct cggcgcagat cctgccaggc
     6361 atcacgtgcg atgccagcta cgccctggcc cggcagcacg acatgccaat cgagctgcgc
     6421 ccggtatccg aagccgagct gcgcagtgcc gatgaactgt ggctcagttc atcgggccgc
     6481 gaagtcctgc caatcacgac gctcgacggc cgccccgttg gcgagggccg tcccggaccg
     6541 atgtacgcac ggatgcggca gtggttccag gcggccaagc gcgccgaggc aaagcactgg
     6601 caggagcgca aggcacgtca ggctcgaggg accctcgcgg catgatcgaa gcgggccgcc
     6661 gcgcgcgcaa gtcggcaccg atgagtggcg cggataagtg acgcggataa gtgctgccga
     6721 tgaacgacgc cgctgaccgc accgagccga aagggctcga gttccccacc gattttccga
     6781 tcaagatcat ggggcgtagc gacgacgctt tcgcgcagtc gattgtcaag gtcgtgctcg
     6841 agcatgcgcc tgacttcgac gcatcgacgc tcgagatgcg cgcgtcgcgg gacggcaact
     6901 acatctcgct gacggctacg atccgcgcgg tatcgcgcga gcaactcgat gcgctgtacc
     6961 gcgcattgac cagccacccg atggtcaaga tcgtcctctg aagcccacct cttgccgagt
     7021 ggaatggccg actgagctcc ctgcgggagc gctgccgcga tgatcgtgcg cgacctcgga
     7081 agatccgagt atctgccgac ctggcacgcg atgcgcgact tcaccgctca ccggaccgaa
     7141 tcgtccgacg acgaattgtg gctcgtcgag catcctccgg tattcacgct cggccaggcc
     7201 gggcgccgcg agcaccttcg gagcgacacg ccgattccgg tcgtgcccac tgatcgaggc
     7261 gggcagatca cctatcacgg cccagggcag gtcgtggcct atccactcgt cgacctgcgt
     7321 cgcctcggca tcttcgtcaa ggcactcgtg ttccgcatcg agcactccgt gatgcagacg
     7381 ctcgccatgt tcgacgtcga cgcgcggcgc gtgcccgggg caccgggggt gttcgtgcca
     7441 tggggcgatg gcctgacgtc gggagagttt gccgggctgg ccaagattgc ggcgctcggc
     7501 atcaaggttt cgcacggccg cagctatcac ggtgtcgcgc tgaatgtggc gatggatctc
     7561 gcgccgtttt cgttgatcaa cccgtgcgga tacgccggac tgccggtgat agaccttgca
     7621 acgctcgggg tctcggcgcg cccggaagca gtttcggccc ggctcgccga gcgcctggtc
     7681 gcgcaccttt cggcttgaac ggcttcctgg ccacacgcga gtcttagcta caattgtcgc
     7741 cttcctaccg tagcgctgct gtgcataagt tccgtgcggc agccttcgcc atctgatcat
     7801 gtcagagcaa agtccaatcg tgcgcgatcc ctacgccaag ctcaggggcg aaggcaagac
     7861 cggcaaggtg ttcaagaaat tcgacatcaa gccggtcccc gcgaacgagg cgctgaagaa
     7921 gccggagtgg atccgcgtca aggccggttc gcctgcaaca cggttctacg agatcaaggc
     7981 tattttgcgc gagcacaagc tgcacacggt gtgcgaggaa gccacgtgcc ccaacatcgg
     8041 cgagtgcttc ggcaagggca ccgcgacatt catgatcatg ggcgacaaat gcacgcgccg
     8101 ttgcccgttt tgcgacgtcg gccatggtcg gcccgatccg ctcgacgccg acgagccggt
     8161 gaacctggcg cggacgatcg cggcactcaa gctcagttac gtggtcatta cctcggtcga
     8221 tcgcgacgac ctgaaggacg ggggcgccca gcacttcgtc gactgcatcc gcgccacgcg
     8281 cgaactgtcg tccgcaacgc ggatcgaggt tctggtgccg gacttccgcg gacggctcga
     8341 gcgcgcactg ggaatcctgg aggccgcgcc acccgacgta atgaatcaca acctcgagac
     8401 cgtaccgcgc ctgtatcgcg agtcgcggcc ggggtccgac taccagcact cgctgacgct
     8461 gctgcaggag ttcaagcggc gagtgccggg cgtaccgacc aagagcggcc tgatggtcgg
     8521 gctcggcgag accgacgacg agatcctgca ggtgatgcgc gacctgcgca cgcatggggt
     8581 cgacatgctg accattggtc agtatctcgc accgacgagt catcatctgc cggtgcgccg
     8641 ctatgtgcac ccggacgtgt tccggatgtt cgagtctgaa gcggcaggca tggggttttc
     8701 gcacgctgcc tgcggcgcga tggtgcggtc gagctatcac gcggaccagc aggcgcacgc
     8761 ggccggcctg gttgacatgt ccatcgccaa aggcgactga gcacacagct ttaggcgatg
     8821 gatcgggacg accgcattca gaatctcctc ctgtccgccg tcggcgagat gccggttcga
     8881 caaggtcccg ttctgagcgg atgatcacca cgctaccttt ccgcgggaca gaaaacctcc
     8941 caccactcga cggcggaggt ccaacgcatc cgccggcact cgaaccatcc tcccagggca
     9001 aacgctgaac ttctaccgat atctgtcgaa ctggtttttt gctctgtcga ggcctcagcg
     9061 ctggggcacg cgcgacgagc cactgtgggc cgaagtcttc agcccggagc gacttgagca
     9121 acacgccgag agccttgcgg aagcacagac aaccggggat ctccgtctgc cctggttcgg
     9181 cttcgcacgg cgcagcgccg ataacgcgcg ggtcctcatc ggcgcttacg aggcaattgc
     9241 tgctgccgtc gacaagcaga gctcgatcac gcctgccgcc gaatggctgc tcaacaactt
     9301 ccacatcgtt gaagaacaga tccgcgacat ccgctccttg ctgccgtcca gtttctttcg
     9361 ggaattaccg gcagttgaca gcggcccact gatcggactg cctcgcatct acggcattgc
     9421 gtgggcgttc accgcgcaca ccgacagccg cttcgactcc ggcctcctgc accgcttcgt
     9481 cgtcgcgtac cagcgcgtac agccgttgac tctggccgag ctatgggcgc tgccgatcat
     9541 gttgcgcatc gtgatgatcg agaatctgcg gcgcctggcg gcccgcatcg tcgaatcgat
     9601 cgacggtcgc gagctggctg atcactttgt cgatcaactg ctcgctctcg gaccgagcgg
     9661 cacgtcaaac gaaccggact ggccgttttc aggggacgtg ccgctgccgc gcgcctccgc
     9721 ggcacagctc tatcagcgct tgcgttactc cgatgcggtg gcagcgccca ttctcaggac
     9781 gctgaccgaa cggctgagca aggaaggcac ccatccggaa gaagtcgttc gactcgaggt
     9841 cgatcggcag acggcggcgg atctttccgt gcgcaatctg atcaccagcc tgcggcaaat
     9901 ttcctcgttc gagtggcaga agttcgtcga ggcggccagc caggtgcacc ttgccctgtg
     9961 cgcggcgccc ctgtactcca gcatggactt catcacccgg gaccgctatc ggcacgccgt
    10021 cgaagaactc gcgcgaaggt ctccactgac ggaacttgaa gttgcgggcg tggcagtcag
    10081 acgcgctgcg gaggccgacg ctgcaacgag cgcgcgcttg ctcgacccgg ggtactacct
    10141 gatcgatgac gggcgggtcg acatcgagag tgagatcagc taccgggttc cgcttgcgcg
    10201 ccaactcttt cgggccgcgc tccggatagc gctgccgatc tacgttggcg ccatcttctt
    10261 tctgacgttc ggcatagtcg ccatcgcgat cgccgcgagt gccgatgcgg gcatcgaggc
    10321 cgccgtcctg ttgctgctcg gagcgctggc gatcttccct gcctccgaca tcgctgtcac
    10381 gatcgtcaac cggctggtcg gcatcgccgt tgcgccccgg cacctgccgc gcctggaact
    10441 cgccggcgga atcccggcat cgatgcgcac gtttgtagtc gtgccgacga tgctcggtag
    10501 cgacgaatcc atcgacgaac aggtccatca gctcgaaacg cgacacttgt cgagcggacc
    10561 gggtgcactc tacttcgcac tgcttaccga ctggcccgat gccgagcagg agcacatgcc
    10621 ggacgaccgc gcccagtatg actacgcggt ggcggccatc caacggctga acagccgtta
    10681 tcccgccaga gcggacgaca gttcggatgg ctaccagcgc ttttacctgt ttcatcgtcg
    10741 acgcctgtgg aatgcctcag agtctcgctg gatgggatgg gagcgcaagc gcggcaagct
    10801 gcaggagttc aatcacctgt tgcgcggcgc gaacgacacg acgtacctta ccgatgagca
    10861 ctatgtgccc ccacccgacg aggtgcgatt tgtcctgacg ctcgacgccg acacgatcct
    10921 gccgatcggc gcggcggccg aactggtggg cacgctcgtc cacccgctga accggccgca
    10981 gctcgacgcg agcggccggg tcgtgcaggg ctacgggatc ctgcagccgc gcatcacgcc
    11041 agcgctgccc acacgcgtcg gcgccacgcg cttccagcag ctgttttccg gaaatgccgg
    11101 cgttgatccg tacacgttcc aggtgtcgga cgtcaatcag gacctgtttg gtgaaggcac
    11161 gttcaccggc aagggcctgt atgacgtgga tgctttcgag acctcacttg ccgggcggac
    11221 gccagagaac gcgatcctga gccatgacct ctttgaagga agcttcgcgc gctgcggttt
    11281 tgtcagcgac gtcgagttgt tcgaggagtt tccatcgcac accggtgtcg cggacatgcg
    11341 cgcgcaccgt tgggtgcgcg gtgactggca gctgctgcca tggatctccg gtcggtactg
    11401 gcacacgatt ccaacgctgg cgcgatggaa gatgctcgag aatctgcggc gctctctgtc
    11461 ggcgccggcc atgttcctga tgctggtggc cagctggctg atccctgcaa tcgcgcccgg
    11521 tccctggatc ggcctggtgc tggtcaccct gctgcttccg cagatgctgc cgctggtaga
    11581 gcgtcttgtg ccgcggccca acctcgaccg cagccattgg ctgcgtagcc tggcgacgga
    11641 tttcgtgtcg ggcggaacgc gtgccggggt caacctggcg ctgcttgccc accgtgcctg
    11701 gctgacgagc gatgcgatcg tacgcaccct ggcgcgcctg gcgaaaagcc gcaatctgct
    11761 cgagtggaac acgagcgcgc aggtgcagtc agcagcgcgt ttgtccgcta ggagctttgt
    11821 caaaagcatg ctgccgagca tggtgatcgt catcgctgtg ggtatcgcgg tgttcaccac
    11881 gcaacccgac catatctgga tcgcgctgcc gcttttcgct ctgtggttgc tggcgccatt
    11941 catcgcaagg aggatcagca cgccgacagt gcacggcgcg gccggatcag tcgcgcctgc
    12001 cgatatcgtc gctctacgac tgatcgctcg tcgcacgtgg ctttacttcg cgacctttgt
    12061 gaacaaagcg aatcacttcc tgcccccgga caatttccag gaggatccgt caccggtcat
    12121 cgctcatcgc agctcgccca ccaacttcgg gctttacctg ctgtccgttg tggcggcacg
    12181 cgatctaggc tggatcagcc tgcacgaaat gacgagccgc attgaggcga ccctggatgg
    12241 tatggatgcc ctgccgcggc atcgtggcca ttttctgaac tggatcgaca cgcttgacct
    12301 gcgtccgctc gaacccaggt atgtatcgac ggtcgacagt ggcaacctgg ccgcaatgct
    12361 gctggtcctc tcgcaggcct gcaaggaggc cctcgatcgg ccactggctc atcagagtgc
    12421 cctggcgggg atcggcgatg aagtgaagct gctgaggcgc tcgctcgcgc gggccgacga
    12481 gagtcgggga gctgccggtg ttggcttcga ccagttgcat gaagcactgc agcgggtcga
    12541 acagttgctc gaagcgacgc ccctggcgcc gcgtgaccgt gatcaacaca tggaagcgct
    12601 cgagtccgcc gtgtcggacg tccacgacat cgtcagcaca ctggccagcg aaggcaacac
    12661 gggccagttg caggaggcct tgctctgggc cgaggcgatg catctgaaca tcaccgcgca
    12721 gcgacgcgaa cggctcgaat tggcgccctg gctggaagcg atcgcgcgcg agcaacgggc
    12781 caccgagacc accgagggcg ccccgtccac tgtgatgcag ccggtagctc aagaagtgtt
    12841 ggccgacatc gatcgttcac gacacgccgt cgaggaactc gccgaccgtc tgaacaccct
    12901 ctcgcgccgc gctcgcctga tgttcgacga gatggatttc cgctttcttt acgacgaaac
    12961 gcgcgatctc ttttcgatcg gctatctggt cgaggatgcg cggctcgacc tcggctgcta
    13021 cgacctgctc gcatcggaag cgaggcttac gagtttcatc acgatcgcga agcgcgacgt
    13081 gcccgcgacg cattggttcc ggctcggccg cacactcgtt ccagtcgacg gcggcgccgc
    13141 gctcgtttca tggtcgggat cgatgttcga atacctgatg ccgtcgctgt tgatggatac
    13201 tccgcacggc agtctgctcg aggcgaccgt gcgccgtgtc atcgaccggc acatcaattt
    13261 tggtcgcgag cgcatggtgc cctggggaat ctccgagtcg gcctacaacg cccgcgatat
    13321 tcacctcacg taccagtact cgaactttgg cgtccccggc ctgggactga agcggggtct
    13381 gtcgaaggac accgtcgtcg cgccctatgc gacagccctc gcggcgatgt acgacgccaa
    13441 agccgccgtg tcgaacttcg agcatctgac cgcagtcggc gccttgggtc gatacggctt
    13501 ctatgaggcg ctcgatttca cgcccagccg attgccgaag tcgaaggagg tggcgatcgt
    13561 ccgggcgtac atggcgcatc accagggcat gaccctggtt gctttgacga acgtcgttct
    13621 caaaggggag atgcgacacc gtttccatcg cataccgatg gtgcaggcga ccgagttgct
    13681 gttgcaggaa accgcgcaac gggatgtcac gctcgccctg ccccgctcag atcgggcgac
    13741 gccggccatc gcaagctcgg cggctgttcc gatcgaccgc agggtcaatt cgccgcacgc
    13801 ggtacctcct gccacgcagt tgctttccaa cggccgctac agcgtgatgg tcacggcggc
    13861 cggatcgggc tatagccgct ggcgcgagct ggccgtgacg cgctggcgag aggacgcgac
    13921 gcgcgattgc tggggtacct acctctatct gcgagatccg gcgagcgggt acctgtggtc
    13981 agccggctac cagccggtgt gcgccgaacc ggtttattac gaggccgagt tcgccgagga
    14041 tcgggtgcgc atcctgcgtg cggacgccgg catcgagtcg tcgatggaga tcatggtttc
    14101 ggccgaagac gacgtggaga ttcgccggtt gacgttgaag aacaccgggt cgcggcggcg
    14161 cgagatcgag atcacttcgt acgcggagat cgtgctcgcc tcgccggcag ccgacgaggc
    14221 tcatccggct ttctcgaatc tcttcgtaca gactgagtat ctgtccgcgg tcaacggatt
    14281 gatcgcgacc cgtcggccgc gctcggccga ggacgaagcg ctgtgggctg cgcacgtggt
    14341 ggcatatgac agcaaggacg acagcagctc gggtatcgag tttgaaacgg accgagtgaa
    14401 gtttctcgga cgcggccgtt cggtacgagc gccgctgtct ttgatggaag gacggcggtt
    14461 gtcgaatacg gtgggcgctg tgctggatcc ggtcgccagc ctgcgcatac gcctcgccat
    14521 cgctccgggg gcgaccgtgc acgcatcatt tgcgacgatg actgccacca gtcgcgaatc
    14581 gattctcaac ctggccgaca agtaccatga tccgggtgca ttcgaccgcg cgtcgacact
    14641 ggcctggacc catgcccagg tcaagctgca ttacctcggc atcgatcccg ccgaggcgca
    14701 ggtctttcaa caactcgcca gtcgcgtcct gtactcggac gcgctgctgc gggccccgcg
    14761 cgaacttctg cagggcaatc gccttccggt ttcgggcctc tggaagttcg gaatttcagg
    14821 cgaccgaccg atcctgctcg cgcgaatcga ggatggcgct gacatcggcg tgatccgcca
    14881 actcctgacc gcgcatgagt atctccatgt gaagcggctc ggaatcgatc tcgtcatcct
    14941 gaacgaacag gcgacctcct acgcggaagg cctgcaacag gatctcgaga tgcgggttcg
    15001 cgaaagcgat gcccgcaagg gcggcgacga tccgggaggg cgtggcacga tctacgttct
    15061 ccgcgccgat cttctcagcg aggacgaacg ggcgctgttg ttgacggccg cgcgcgtcgt
    15121 cgttgtctgc ggccaaggca acttgtctgc gcacctggcg atgcggcacc gtgagcctgt
    15181 cctgcaagtc cagccgcgcg ttcacgcggc gccaagcacg tcaaaactgc tacatccgcc
    15241 tcccctgcag tttccgaacg ggctgggcgg ctttaccgaa agcgggcgcg agtacgccgt
    15301 cgtcctgaat cgcggccagc gcacgccact tccatggatc aacatcatcg ccaacgacgg
    15361 attcggattc cacgtctccg agtcaggtgc tggctacacg tgggcgggca acagtcggga
    15421 aaaccaattg acaccctggt ccaacgatcc ggtgtccgac cccgttggcg aagcgttcta
    15481 ccttcttgat cgcgactctg gcgtcctgtg gacaccgacc gccctgccga tccgcgtcga
    15541 ggactccagc tatatcaccc gcttcggcca cggctacgca cgctttcagc atcggtcgaa
    15601 cggtatcgag agcgaattgg tccagttcgt cgcctccgag gatccggtca agatctcccg
    15661 gctccggctc atcaatcact cgagccagac acgccacatt tcgattaccg cctacgtcga
    15721 gtgggtgctt ggattctcgc gcaccgtcaa tgcaccgttc atcgtcaccg aggccgaccc
    15781 ggagacgggc ggagtattcg cgttcaatcc gtggcatcgc gagttcggca accggatcgc
    15841 ttttgtggca tgggatgcgc cggccgatgg tctgacctgc gatcggcagg agtttgtcgg
    15901 acgcaacgga catcttgaag caccggttgc gctgaccgcc gacgtttcgc tttcgggacg
    15961 gtggggcgcg ggattcgatc catgcgtcgc gctgcagcgg gaagtgcaga tcgagccaga
    16021 gagccagatc gaactgacct tcgtgctggg ccaggcggcc gacgccgacg ctgcgcgagc
    16081 gctgattcga cgctttcgga ccgtgaaaga ggctgacgcc gagttacttc atgtgaagca
    16141 gcagtggcgc gagatcctcg accgcgtgca ggtcgagacg ccggatcccc agatgaatct
    16201 ggtcctcaat cactggctgc tgtaccagac gatcgtctgc cggctgtatg cccgtagcgc
    16261 gttctaccag gccggtggtg cgtatggatt ccgcgaccag cttcaggacg tcatggctct
    16321 gacgatcaca cgaccggatc tgacgcgcag gcagatcgtc aacgcctcat cgcatcagtt
    16381 ccgtgaaggt gacgttcagc attggtggca tccgcctttc gggcgcggcg tgcgaacgca
    16441 tttttccgat gatcgcgtct ggctacccta ctgcgtcagc cattatctga ccaccaccgg
    16501 cgatacgggc gtgctggatg agagggtgcc attcgtcgaa ggcgacctcc tgccgcccga
    16561 gagcgaggcg atgtacttcg agcccgccat ctcggatgag aaggtcacgg tgtacgagca
    16621 ctgtgcccgc gcgctcgacg tgagcatggc gatcgggcag cacggcctgc cgctgatggg
    16681 cggcggagat tggaacgacg gcatgaaccg cgttggtgcc gaaggccgtg gggagagcat
    16741 ctggctggcc tggttcctgt gtgcggcgtt gcgcgagttc gcacccgtgg ccgcacagcg
    16801 cggcgaactc gaacgcgcac gcgcgtggtc cgaccacgca gaccgccttc aaacggccgt
    16861 cgaacacgag gcctgggatg gcgcctggta tcgccgcgcg tacttcgacg acggcacccc
    16921 gctgggtgcc tcgggcaacg ccgaatgccg catcgattca attgcgcaga gttgggcgct
    16981 gatatccggc gcgggtgaac cggcgcgtgc gcgccgagca atggaatccg ttggcgaaca
    17041 cctggtcaag ccgggcgaca gcatggtgct gctgttcgca ccgcccttcg accgatcggc
    17101 acaagagccc ggatacatca agggctacct gccgggagtg cgcgaaaacg gcgcgcagta
    17161 cacacaggcc gcggtctggt gcgtgatcgc ccatgcgctg cagggtgacg gcgagcgtgc
    17221 tgcggagtta ttcgagatgt tgaatcccat caaccacagc tcgacgcgcg ccggaattca
    17281 tcgctacaag gctgaaccct tcgttatcgc cgccgacgtg tacagcgaac caccgcatac
    17341 cggacggggt ggctggagct ggtacaccgg cgccgcggga tggatgtatc gcgctggcat
    17401 cgagttcatc ctcgggttca agctgcgcgg tcgtgaaatc ctgattgatc cttgcattcc
    17461 cgagcagtgg cgcagcttca agattcgcta ccgtgcgccg cagtcgacgt acgagttcgt
    17521 catcgacaac ccgcagggcg ttgcacgcgg cgtccttagg
//
LOCUS       DATIRT010000143         6389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_17087, whole genome shotgun sequence.
ACCESSION   DATIRT010000143 DATIRT010000000
VERSION     DATIRT010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6389
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17087"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VLN25_03450"
     CDS             complement(<1..283)
                     /locus_tag="VLN25_03450"
                     /inference="COORDINATES: protein motif:HMM:NF018391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HSQ09662.1"
                     /translation="MKVFNLSCENEHPFEGWFASADDFDRQLQVKLVECPVCGSHAVR
                     KLLSAPRLNLGAEAPETNAKQAVAMPNDATMQAMLLQMSRHIHANSEDVG"
     gene            complement(293..383)
                     /locus_tag="VLN25_03455"
     tRNA            complement(293..383)
                     /locus_tag="VLN25_03455"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(347..349),aa:Leu,seq:taa)
     gene            complement(509..1810)
                     /locus_tag="VLN25_03460"
     CDS             complement(509..1810)
                     /locus_tag="VLN25_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HSQ09663.1"
                     /translation="MNELSIHSASPSWRTPLGLVLFVSVTTLLGACSLVSPPAPAPVV
                     TPPAPVTSPAPAPATVAAPSPPPIPVQPLDTALLDAAGKLFSAASSSLGAPPAQRLLV
                     IDPLVDAVSYTQSVTTQSMEAKIQGLVRSTYPGFNVQPISRASIGQAPIVLVGTFTAI
                     DLKNQVGGQREAFRICLALADLKSGLIVAKGVARAQVEGIDQTPLQHLQDAPTWAKDP
                     AVDAYVRTCQTTKPGDRIDPAYLDGILAASLIAEASALQDRGRYQEAIDVYRSARATP
                     KGDQLRVHNGIYSASWKLGKRADAEQAFGEAVRVGLASNRLGVKFLFRPGSTDFVSDP
                     RLSAPYPIWITQIARRSAAQSACLNVVGHTSPTGPEPLNERLSVLRAENVKNRLERET
                     PQLKGRVIASGVGSKEKLVGTGTDDLRDALDRRVEFKTVPC"
     gene            complement(1807..2916)
                     /locus_tag="VLN25_03465"
     CDS             complement(1807..2916)
                     /locus_tag="VLN25_03465"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HSQ09664.1"
                     /translation="MADVFVSYSRQDKARAAQFVQLLEGFGWDVFWDQETRAGTIWPK
                     VLEDELGLARCLLVLWTANSVASRWVRIEAYEALQNDKLLPVRLENVKPPMEFRQTQT
                     FDLLGWAGDHNDPRLDHLIADFCVLTKLEPRKGTVGPATLNVVRTLVPATPGANALAP
                     ATLSGAPDLRAHVVPTDRTVGLATPTLVPTEPAHVTDTEVAKGATAQADEVRSAIHAG
                     QTTAVLTGETDAVDTARITPDPLRRRMGWGLGAAVISIALVWVAVQILKPHKAAEPDP
                     FKPNMTATVPNPNPPTGPATASVPPSTAAATSAPTSSAAPTVEVSRPVTVAKPPATRP
                     PAAFNRCIEITERFQQTGQITVEDRQFLSSKECSR"
     gene            complement(3269..3342)
                     /locus_tag="VLN25_03470"
     tRNA            complement(3269..3342)
                     /locus_tag="VLN25_03470"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3308..3310),aa:Cys,seq:gca)
     gene            complement(3393..3468)
                     /locus_tag="VLN25_03475"
     tRNA            complement(3393..3468)
                     /locus_tag="VLN25_03475"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3433..3435),aa:Gly,seq:gcc)
     gene            complement(3524..4096)
                     /gene="pgsA"
                     /locus_tag="VLN25_03480"
     CDS             complement(3524..4096)
                     /gene="pgsA"
                     /locus_tag="VLN25_03480"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HSQ09665.1"
                     /translation="MAFNVPMALTWARIAMIPVIVGIFYVPDSWFPSHWKNATACWLF
                     VLAAITDAIDGYVARKYGMVSRLGAFLDPVADKLMVSAALIVLLSLGRIDEFVALVII
                     GREIAVSALREWMAQIGKSASVAVNSLGKIKTIAQMVAIPMLLFHDKLFGVIDVHLIG
                     TLLIYVAAALTVYSMFVYLRMAWPHLDNES"
     gene            complement(4116..5966)
                     /gene="uvrC"
                     /locus_tag="VLN25_03485"
     CDS             complement(4116..5966)
                     /gene="uvrC"
                     /locus_tag="VLN25_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HSQ09666.1"
                     /translation="MSDTSASFDPRAVVDGLPHLPGVYRMFDAEGGALYVGKARDLRK
                     RVASYFNKGVGPRTAHMLARVTRIDTTVTRSEAEALLLENNLIKSLAPRYNIVFRDDK
                     SYPYLKFTSHAFPRVAYYRGAVDRRAQYFGPFPNAWAVKDSIQLLQKVFRLRTCEDTV
                     YANRSRPCLLHQIQRCSAPCVGLIDEATYAQDVDRAVRFLRGETDEVMRELEGRMQQL
                     ATDLKFEEAAAVRDRLGALSRVLHQQAVETTGSDADADVLAVVTDGGLACVNLAMIRG
                     GRHLGDKAIFPARSGDVVEHGEILEAFISQHYAVQPCPPTIVVNADIDADDVSAFLSE
                     ASGRRVQLVRHPQESRRRWLEMAEANARLALARRLAEEGSQMARTRALIEALGLDIGD
                     DDADKLRVECFDISHTSGEATQASCVVFANHQMRPSEYRRYNIEGIEPGDDYAAMRQV
                     LSRRFAAVARGEGVLPQLALIDGGKGQVMVARQVFEELGLDTGVLVGVAKGEERKVGL
                     EELVYPDDRPSLVLGRESQALMLVAQIRDEAHRFAITGMRARRAKTRQNSQLETMDGI
                     GPKRRQRLLARFGGLRGLAAASVDDIATVEGVSRKLAERIYAQLHGQPTE"
     gene            6100..>6389
                     /locus_tag="VLN25_03490"
     CDS             6100..>6389
                     /locus_tag="VLN25_03490"
                     /inference="COORDINATES: protein motif:HMM:NF019814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HSQ09667.1"
                     /translation="MAKILATEIRAGNLLEFDKRVWRVLKSYHVHVGGRGGAYMQIEI
                     KDIESGQKRNERLSTDEKIERPFIDRRTMTYSYHDGDNYVFMDNETYEQLTLG"
BASE COUNT         1170 a         2053 c         2038 g         1128 t
ORIGIN      
        1 cgccgacatc ctcgctattc gcatggatat ggcgtgacat ttgcagcagc atggcctgca
       61 tcgtcgcgtc gttcggcatc gcgaccgcct gctttgcatt cgtctcgggc gcctcggcac
      121 cgagattcag gcgcggcgcc gacagcagct tgcgcacggc atggctgccg cagaccgggc
      181 actcgaccag tttcacctgc aactgccgat cgaagtcatc ggccgaggcg aaccagccct
      241 cgaacggatg ctcgttttca cagctgaggt tgaatacttt catcggtcgt gatggtgccg
      301 cgggccggaa tcgaaccggc atgcttgctt tcgcttgcgg cggattttaa gtccgctgtg
      361 tctaccagtt tcaccaccgc ggcaggtggc ggcctggcac agcgtatcag cgtccgggag
      421 tgcagccccc gggttcaatc ggtcggtgtg atatgccgtc gaatcctatt tcaacggcaa
      481 tgcgatcgac tccagtttcg aggttaccct aacatggcac cgtcttgaac tcgacgcgac
      541 gatcgagcgc gtcgcgcaga tcatcggtac ccgtgccgac aagtttctcc tttgatccga
      601 cgccactcgc gatgacgcgg cccttgagtt gcggcgtttc gcgttcaagg cggttcttca
      661 cgttttcggc gcgcaggacc gacagacgtt cgttcaacgg ctcgggaccg gtcggactgg
      721 tgtgtccgac gacgttgagg caagccgact gcgccgccga gcggcgcgcg atctgtgtaa
      781 tccagatcgg atacggcgcg ctcaggcgcg ggtccgacac aaagtcggtc gagccaggcc
      841 ggaacaggaa cttcactccg agccggttgc tggcgagccc gacgcgcacc gcctcgccga
      901 atgcctgctc cgcgtcggcg cgcttgccaa gtttccagct tgcactgtag atgccgttgt
      961 gaacgcgcag ctggtcgccc ttcggcgtag cacgcgcgct gcgatagaca tcgatcgcct
     1021 cctggtaacg accccggtct tgcagggcac tggcttccgc gatcagtgaa gcggcgagga
     1081 tgccgtcgag atacgcagga tcgatacggt cgccgggctt ggtcgtctgg caggtccgca
     1141 cgtaggcatc gacggccgga tccttggccc aggtcggcgc gtcctgcaga tgctgcagcg
     1201 gagtctggtc gatgccctcg acctgcgcac gcgccacacc cttggcgacg atgagtccgg
     1261 acttcaggtc agccagggcc aggcagatgc ggaacgcttc gcgctggcca ccgacctggt
     1321 tcttcaggtc gatcgcggta aatgtgccga ccagcacgat cggggcctgt ccgatgctgg
     1381 cgcgtgagat cggctgcaca ttgaagcccg gatacgtgct acgcaccagc ccctggatct
     1441 ttgcctccat cgactgtgtc gtgacgctct gtgtgtagga cacggcatcc acaagcggat
     1501 cgatgaccag caggcgttgc gccggtggcg caccgagtga gctcgatgct gctgaaaaca
     1561 gcttgccggc ggcgtcgagc agtgccgtgt cgagtggctg cactggaatc ggcggcggag
     1621 agggtgcggc aaccgtagca ggcgcgggcg ccggcgaagt gaccggcgca ggcggcgtca
     1681 cgaccggagc gggagcgggc ggcgacacca aggagcatgc gcccagcagg gtcgtcacgc
     1741 tgacgaacag cacaagtccc aacggggtgc gccaggaagg tgatgcggaa tgaatggaga
     1801 gttcgttcat cgtgagcact ccttgctgct gaggaactgc cggtcttcga cggtgatctg
     1861 gccggtctgc tggaatcgct cggtgatttc gatgcagcgg ttgaaagcgg caggcggacg
     1921 cgttgcggga ggcttcgcga cagtcaccgg ccggctgact tcgactgtcg gcgccgcaga
     1981 cgatgttggt gccgacgttg ctgctgctgt cgatgggggc accgaggcag tcgctggtcc
     2041 ggtgggcgga ttcggattgg gcacggtggc ggtcatgttg ggcttgaacg gatccggttc
     2101 ggcagccttg tgcggtttga gaatctgaac ggcgacccat acgagggcga tcgagatgac
     2161 tgcagctccc aggccccacc ccatacgccg gcgcaaagga tccggcgtga tcctcgctgt
     2221 gtcgactgcg tcggtctcgc cggttaatac cgctgtggtc tgcccagcat gaattgcgga
     2281 gcgcacttcg tccgcctgcg cggtcgcacc tttcgcgacc tctgtgtcgg tgacgtgcgc
     2341 gggttcagtc ggaaccagtg tcggggtggc caatccaacg gtgcggtcgg tcggcacaac
     2401 gtgtgcgcgc aagtcgggcg cgcccgacag cgtggcaggg gccagcgcat tcgctcccgg
     2461 cgtcgcagga acgagcgttc gtacgacatt caatgtcgca gggccgaccg tccccttgcg
     2521 cggttcgagc ttggtcagca cacaaaagtc cgcgatcaag tgatccagcc gcggatcatt
     2581 gtgatcgccg gcccaaccca gcagatcgaa ggtctgcgtc tgccgaaact ccatcggcgg
     2641 cttgacattc tcgagacgca ccggcagcaa cttgtcgttc tgcagcgctt cgtacgcctc
     2701 gatgcgtacc cagcggctcg ccaccgagtt ggcggtccac agcacgagca gacagcgcgc
     2761 aaggcccagc tcgtcttcca ggaccttcgg ccagatcgtt cccgcgcggg tttcctggtc
     2821 ccagaagacg tcccaaccga agccttccag caactggaca aactgcgccg cgcgcgcctt
     2881 gtcttgacgc gagtagctaa cgaagacgtc cgccattgcc acctctgcct gcgccctgcg
     2941 gaacggtcgg gctcgacacg atcacaggga accttgccgg aagccacagt ctaggagcga
     3001 tgtgcgccag cagctaccgc atattcatgc gcccatagtt tccagggctt cgtagccgag
     3061 acagtcgtcg ttctacttcg gggccgccac agaccgaacc tgccagcgat cgcttgcggc
     3121 gatccggtca gcgtcgaccg gaatgtcgga gaggactcgc acgacagtcc gggcgaccga
     3181 atctcgatga ccggcttcgg ctcggatccg gaacctacgg atagcatgag cgaaagtcgc
     3241 cgccgggagg cgcgcacttt caactcattg gaggcggggg tcggaatcga accggcgtac
     3301 gcggatttgc agtccgctgc atggccactc tgccaccccg ccgcatgggg ttcaaaccaa
     3361 aagggggaag cgcgtggctc cccccagaat tctggagcgg gcgaagagtc tcgaactctc
     3421 gacctcaacc ttggcaaggt tgcgctctac caactgagct acgcccgctc gaagcccgga
     3481 attatatcgg cggacgaagg ggtgtcaaac cacgcactaa ctttcagctt tcgttatcga
     3541 gatgcggcca cgccatgcgc agatagacga acatggaata cacggtgagc gcagccgcca
     3601 cgtagatcag cagcgtgccg atcaggtgca cgtcgatgac gccgaacagc ttgtcgtgga
     3661 agagcagcat cgggatcgcg accatctgcg caatggtctt gatcttgccg agcgagttga
     3721 cggccacgct tgcggacttc ccgatctgag ccatccactc acgcaaggca gacacggcta
     3781 tttcgcggcc gatgatcacc agtgccacga actcgtcgat gcggccgagc gacagcagca
     3841 cgatcagcgc cgcactgacc atcagcttgt ccgccacggg atccagaaag gcacccaggc
     3901 ggctgaccat gccgtacttt cgcgcgacat aaccgtcgat cgcatcggtg atcgcggcca
     3961 gcacgaacag ccagcacgcc gtcgcgttct tccagtgcga tggaaaccag ctgtccggga
     4021 cgtaaaagat gccgacgatg accgggatca tcgcgatgcg cgcccaggta agcgccatcg
     4081 ggacattgaa ggccagcggt gggctgctcg atggactatt cggtgggctg gccatgcagt
     4141 tgcgcgtaga tcctctccgc cagcttacgc gaaacgcctt cgacggtcgc aatgtcgtcg
     4201 accgacgccg cggccagtcc gcgcagtcca ccgaagcgcg cgagcaggcg ctggcgccgc
     4261 ttgggaccga taccgtccat ggtttcgagc tgggagttct ggcgcgtctt ggcacggcgc
     4321 gcgcgcattc ctgtaatcgc aaagcggtgc gcctcgtcgc ggatctgggc gaccagcatc
     4381 agggcctgcg attcacgccc caggacgagc gaaggccgat cgtcgggata cacgagttcc
     4441 tcgagcccga ccttgcgctc ctctcccttg gcgacgccaa ccagcacgcc ggtgtccagc
     4501 ccaagttcct cgaagacctg gcgcgcaacc atgacctggc ccttaccgcc gtcgatcagc
     4561 gccaactggg gcagcacacc ctcgccgcgt gcgaccgccg cgaaccgtcg cgacaggacc
     4621 tggcgcatcg ccgcgtagtc gtcgccgggt tcgatgccct cgatgttgta gcgccggtac
     4681 tccgagggcc gcatctggtg gttcgcgaac accacacagg aggcctgtgt cgcctcgccg
     4741 gacgtatggc tgatgtcgaa acactcgacg cgcaacttgt ccgcgtcgtc atccccgatg
     4801 tcgaggccga gtgcttcgat cagcgcgcgc gtgcgggcca tctgcgaacc ctcttccgcc
     4861 aggcggcgcg ccagggccag tcgggcattg gcctcggcca tttccagcca gcgccgccga
     4921 ctttcctgcg gatgacgcac cagttgaacc cgccggccgc tcgcttccga aagaaaggcg
     4981 ctgacgtcgt cggcatcgat gtcggcattc acgacgatgg tcggcgggca cggctggaca
     5041 gcgtagtgct gcgagatgaa cgcttcgaga atctcaccgt gttcgacgac atcgccgctg
     5101 cgtgcgggga agatggcctt gtcgcccagg tgccggccac cgcggatcat cgcgaggttc
     5161 acgcacgcga gcccgccgtc cgtgaccacg gccagtacgt cagcgtcggc gtccgagccg
     5221 gtggtctcca cggcctgctg gtgcagtacg cgagagagcg caccgagccg gtcgcgcacg
     5281 gccgcggcct cctcgaactt gagatccgtc gcgagctgct gcatgcggcc ctcgagttcg
     5341 cgcatcacct cgtcggtttc gccccgcagg aaccgcaccg cgcgatcgac atcctgcgca
     5401 tacgtggcct catcgatcag tccgacgcac ggagcactgc agcgctggat ctggtgaagc
     5461 aggcagggac gcgaacgatt cgcgtagacg gtgtcttcgc aggtccgcag ccggaaaacc
     5521 ttttgcagca gctggatgct gtccttcacc gcccatgcgt tcgggaaggg cccgaagtac
     5581 tgcgcgcgtc gatcgacagc accgcggtag tacgccacgc gcggaaacgc gtgactggtg
     5641 aacttcaggt acggatacga cttgtcatcg cgaaacacga tgttgtatcg cggcgcgaga
     5701 gacttgatca ggttgttctc gagcagcagc gcctcggcct cggaccgggt caccgtcgtg
     5761 tcgatccgcg tcactcgcgc cagcatgtgc gccgtgcgcg gcccgacccc cttgttgaaa
     5821 tacgacgcca cccgcttccg gaggtcgcgt gccttgccga cgtacagtgc accaccctcg
     5881 gcgtcgaaca tgcgatagac gccaggcagg tgtggcaggc cgtcaacgac ggcgcgggga
     5941 tcgaaggaag cggaggtgtc agacatgatc gaagtcgcgt cgcagcatat aatggcgggc
     6001 tttcggcgtc ccagccagca gcggccagca acaccggccg cagagcccgc tgagtcctca
     6061 gcgcaaaccc cgagtcaaca caaaccacga gtcaccctca tggccaagat ccttgccaca
     6121 gaaatccgtg ccggcaacct gctggaattc gacaagcgtg tctggcgcgt gctcaagagc
     6181 tatcacgtgc acgtcggtgg ccgtggcggc gcctacatgc agatcgagat caaggacatc
     6241 gagagcggcc agaagcgcaa cgagcgcctg tctaccgatg aaaagatcga gcggccgttc
     6301 atcgatcgcc gcacgatgac ttactcgtac cacgatggcg acaactacgt tttcatggac
     6361 aacgagacct acgagcagtt gaccctcgg
//
LOCUS       DATIRT010000144         1618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_410718, whole genome shotgun sequence.
ACCESSION   DATIRT010000144 DATIRT010000000
VERSION     DATIRT010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410718"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="VLN25_03495"
     CDS             <1..1039
                     /locus_tag="VLN25_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HSQ09668.1"
                     /translation="TRKQLNAAIRDTGLFFPIDPGADATIGGMAATRASGTNAVRYGT
                     MRENVLALTVVTAEGKVIRTASRAKKSSAGYDLTRIFVGSEGTLGVITEVTLRLYPQP
                     EAISAATCSFPSVDAAVQTTIATIQLGVPIARCELLDALTVKAVNQRDKLGLPELPLL
                     LFEFHGSPASVEEQARTVEAIAAEHGGQGFKWATKPEDRTRLWEARHNAYFACLQLKP
                     GCRSFTTDVCVPISRLAECITETIADTEASFLPAPILGHVGDGNFHCAILANADDPRE
                     VEEAERLNTRIVERAIAMDGTCTGEHGVGMHKMEFLRDEHGDDALDLMVRLKRAFDPL
                     NILNPGKIVRV"
     gene            complement(1075..1356)
                     /locus_tag="VLN25_03500"
     CDS             complement(1075..1356)
                     /locus_tag="VLN25_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09669.1"
                     /translation="MLVRGLGTAFALLFPLVLLAQRRSLPELAARLVGCADRAYAEGG
                     RYLHPPEKRMRDSVLAALRASLSDDSIAALQAEGARWSEDEGFASAGIH"
     gene            complement(1399..>1618)
                     /locus_tag="VLN25_03505"
     CDS             complement(1399..>1618)
                     /locus_tag="VLN25_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09670.1"
                     /translation="EAGLQNVALARAAGALGAIPALSKLADTEFVLGHSEAAIALCRE
                     AIERAAAIGRPAAAVHAYGNMVPALLER"
BASE COUNT          290 a          555 c          520 g          253 t
ORIGIN      
        1 cactcgcaag cagctcaatg cagcgatcag ggacacggga ctgttcttcc cgatcgaccc
       61 gggtgcggat gcaaccatcg gcggcatggc ggcgacgcgc gcctccggca ccaatgcagt
      121 ccgctacggc acgatgcgcg agaacgttct ggcactcacg gtcgtcaccg cggaaggcaa
      181 ggtcatccgc actgcaagtc gcgcgaaaaa gagctcggcc ggctacgacc tgactcgcat
      241 ttttgtcggc tccgaaggca cacttggcgt aatcaccgag gtgacgctgc ggctgtaccc
      301 gcagcccgag gcaatctcgg ctgcgacctg ctcgtttccg agtgtcgatg ccgcagtgca
      361 gacgaccatc gcgacgatcc agctcggagt gccgatcgcg cgctgcgagt tgctggacgc
      421 cctgaccgtg aaggcggtca accagcgcga caagctgggc ctgcccgaat tgccgcttct
      481 tcttttcgag ttccacggct cgcccgccag cgtcgaggag caggcgcgga cggtcgaggc
      541 gatcgccgcc gagcacggtg gacagggctt taaatgggcc acgaagcccg aagaccgcac
      601 gcgactatgg gaagcgcgcc acaacgccta tttcgcgtgc ctgcagctca agcccggatg
      661 ccgcagcttc acgaccgatg tctgcgtgcc gatctcacgc ctggccgagt gcatcaccga
      721 aacgatcgcc gacaccgagg cgagctttct gccggcaccg atactcggcc atgtgggcga
      781 cggcaacttc cactgcgcca ttcttgcgaa cgccgatgac ccgcgcgagg tcgaggaggc
      841 cgaacgcctc aacacgcgca tcgtcgagcg cgcgatagca atggacggca cctgcaccgg
      901 ggaacacggc gtcggcatgc acaaaatgga gttcctgcgc gatgagcatg gcgacgatgc
      961 gctggatctg atggtccggc tgaagcgggc gttcgatccg ctcaatatcc tgaaccccgg
     1021 gaagatcgtg cgcgtgtagc ggttgactga gccgccgggc gcacgccgag ccgttcagtg
     1081 gatgccggcg ctcgcgaatc cttcgtcttc gctccagcgc gccccctcgg cctgcaacgc
     1141 ggcgatcgag tcgtcactga ggctggcacg caacgctgcg aggacactgt cacgcatcct
     1201 tttctcgggc ggatgcaggt agcgtccgcc ctcggcatag gcgcggtccg cgcaaccgac
     1261 cagcctcgcc gccagttccg gcaggctgcg acgttgtgcc agcaatacga gaggaaagag
     1321 cagggcgaac gccgtaccga gaccgcgtac cagcaacgct cgtccggcgc gtatcgcctc
     1381 ctcagcctgg tccagttctc agcgttcgag cagcgccgga accatgttgc cgtaggcatg
     1441 cacggccgcg gccggccgcc cgatggcagc cgcgcgttcg atcgcctcgc ggcacagcgc
     1501 gatcgccgcc tcgctgtggc cgagcacgaa ctcggtatca gcgagtttgc tgagcgcagg
     1561 gatcgcgccc agcgcgcccg ccgcgcgcgc cagcgcgacg ttctgcagtc cggcctca
//
LOCUS       DATIRT010000145         1852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_115399, whole genome shotgun sequence.
ACCESSION   DATIRT010000145 DATIRT010000000
VERSION     DATIRT010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115399"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..>1852
                     /locus_tag="VLN25_03510"
     CDS             174..>1852
                     /locus_tag="VLN25_03510"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HSQ09671.1"
                     /translation="MSKALIIAEKPSVAADIARALGGFTKQQDFFESDRYVLSSAVGH
                     LLELKAPESHDVKRGKWTFTHLPVIPPHFDLAPIARAEARLKVLLKLLKRKDVTELVN
                     ACDAGREGELIFRYIVQAAKARQPVRRLWLQSMTPASIREAFESLRSDDEMLPLADAA
                     RSRSEADWLVGINGTRAMTAFNSKEGGFYLTTVGRVQTPTLAIVAEREDKIRRFVSRN
                     YWEVRAKFDARAGRYDGRWFDEAFRKADDPDARAERLWDESRAAAIVAACTGKPGDVS
                     EEAKPSTQLSPLLFDLTSLQREANGKFGFSAKTTLSIAQALYEKHKVLTYPRTDARAL
                     PEDYLGVVKKTMEELGAVSAYKPFTKQALKAGWIRPNKRVFDNSKISDHFAIIPTPQT
                     PGHLSEVEAKLYDLVAKRFLAVFFPAAEYLVTTRITRVEGHAFKTEGKVLVEPGWLAI
                     YGREAVEEQGSLVKVDDGERVRTTEIESVPLATRPPPRYTEATLLTAMEGAGKLIEDD
                     ELRAAMAGKGLGTPATRAQIIENLIDQRYMVRDGRDLRPTRKAFDLMTLLKG"
BASE COUNT          355 a          609 c          593 g          295 t
ORIGIN      
        1 ccgccaagcg cgaggctgat gccaccatac ataactggac accgcgcagt aggcgatttc
       61 tcccgccggg ccgggcactt gcatcggttt tcgatttcgt tgttatcctg aaaaaagaga
      121 gccgagctca tatatgagga ccggccgcct gtgatggcgg cctaattttc tgcatgtcca
      181 aagcactgat cattgccgag aagccctccg tcgccgccga tatcgcgcgc gcgctgggcg
      241 gcttcaccaa gcaacaggac ttcttcgaga gcgaccgcta cgtgctgtcc tccgcggtag
      301 gccacctgct cgaactcaag gcgcccgagt cgcatgacgt gaagcgcggc aagtggacct
      361 tcacgcacct gccggtgatc ccgccgcact tcgacctcgc acccattgcc agagccgagg
      421 cgcgcctcaa ggtgctgctc aagcttctca agcgcaagga tgtcaccgag ctcgtcaacg
      481 cctgtgacgc cggacgcgaa ggcgaactga tcttccgcta catcgtgcag gccgcgaaag
      541 cgcgccagcc ggtgcgccgt ctctggctgc agtcgatgac gccggcctcg atccgcgagg
      601 cgttcgagtc cctgcgcagc gatgacgaaa tgctgccctt ggccgatgca gcgcgcagcc
      661 gctccgaggc cgattggctg gtggggatca acggcacgcg cgcaatgacc gccttcaaca
      721 gcaaggaagg cggcttctac ctgacaacgg tgggtcgcgt gcagaccccg acgctggcga
      781 tcgtcgccga gcgggaagac aagatccggc gctttgtttc gcgcaactac tgggaagtgc
      841 gcgcgaagtt cgacgcccgg gccgggcggt acgacgggcg ctggttcgac gaggcgttca
      901 ggaaggccga tgacccggat gcccgcgccg agcgtctgtg ggacgagtcc agggccgccg
      961 ccatcgtcgc tgcctgtacc ggaaagcccg gcgatgtcag cgaggaagcc aagccgagca
     1021 cgcagctgtc gccgctgctc ttcgacctga cgtcgctgca gcgagaggcc aacggcaagt
     1081 tcggcttttc ggcaaagacg acgctatcga tcgcgcaggc gctttacgag aagcacaagg
     1141 tgctgacgta tccgcggacc gatgcccgcg cgctgcccga ggattacctc ggcgtggtga
     1201 agaagacaat ggaggaactc ggcgcggtgt cggcgtacaa gcccttcacg aagcaggcgc
     1261 tgaaggcggg ctggatccgt cccaacaagc gtgtgttcga caactcgaag atctcggacc
     1321 acttcgcgat catccccacg ccccagactc ccggacatct gtccgaggtc gaagccaaac
     1381 tttacgacct cgtcgccaag cgctttctgg ccgtgttctt tccggcggcc gagtacctgg
     1441 tgacgacgcg catcacccgg gtcgagggcc atgccttcaa gaccgagggc aaggtgctgg
     1501 tcgaacctgg ctggctcgcg atctacggcc gcgaggccgt cgaggaacag ggctcgctgg
     1561 tcaaggtcga cgacggggag cgcgtccgca cgaccgagat cgaatccgta ccgctcgcca
     1621 cgcgcccgcc gccgcgctac acggaagcga cgctgcttac cgcgatggaa ggagccggca
     1681 agctgatcga ggacgacgag ctgcgcgcgg cgatggccgg caaagggctc ggcacccccg
     1741 ccacgcgcgc gcagatcatc gagaacctga tcgaccagcg ctacatggtc cgcgatggcc
     1801 gggacctgcg ccctacgcgc aaagcgtttg acctgatgac cctgctcaaa gg
//
LOCUS       DATIRT010000146         4505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1591132, whole genome shotgun sequence.
ACCESSION   DATIRT010000146 DATIRT010000000
VERSION     DATIRT010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591132"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1311)
                     /locus_tag="VLN25_03515"
     CDS             complement(<1..1311)
                     /locus_tag="VLN25_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HSQ09672.1"
                     /translation="MTSKSTPTDGDALLQALTAARAPLTPQELADRLHVADDARPAFN
                     TELEELERAGRVVRNRAGLLLVAARANLISGQVQGHRDGFGFLIRDDAGQDLVLSEHE
                     MSKVLHGDRVLARVSGTDRRGRPEGEIVEVIESRTNKLVGRLLNERGVTIVVPEDQRI
                     QHDILVPPGATLAAEPGQVVVVEILQQPQRYVQPVGRVIQVLGAIDDPGMEIEIAVRK
                     FSVPHEFPAEALALADSLPDGVQPRDLRGRVDLRDVPLVTIDGEDARDFDDAVYCEPI
                     AGRKKGWRLIVAIADVSHYVKSGDALDTEAQNRSTSVYFPRRVIPMLPEKLSNGLCSL
                     NPSVDRLVMVCDCVISAQGALDAYQFYPAVMHSHGRLTYDEVWEALSVPGSRSAHRLV
                     DLLPHLQNLYSLFETLASAREARGAIDLETTETYIVCDVNGRIEK"
     gene            1419..1503
                     /locus_tag="VLN25_03520"
     tRNA            1419..1503
                     /locus_tag="VLN25_03520"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1453..1455,aa:Leu,seq:cag)
     gene            complement(1701..2237)
                     /locus_tag="VLN25_03525"
     CDS             complement(1701..2237)
                     /locus_tag="VLN25_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HSQ09673.1"
                     /translation="MDRSVVKDLYVTWDEYHRLIERLSLAIHESGWQFDSLLCLARGG
                     LRVGDIISRIYDMPLAILAASSYREAAGRSQGELDIGKYITMTQGTLKGRLLLVDDLV
                     DSGVTLGRVVEHLKERFPELDEVRTAVIWHKACSTFNPDYHVEYLPTNPWIHQPFEVY
                     DAMGPQNLAARLRGPASG"
     gene            complement(2251..3543)
                     /locus_tag="VLN25_03530"
     CDS             complement(2251..3543)
                     /locus_tag="VLN25_03530"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HSQ09674.1"
                     /translation="MAKNVVVVGTQWGDEGKGKIVDWLTEDVDGVVRFQGGHNAGHTL
                     VIAGKKTILRLIPSGILRPKTACYIGNGVVLSPPALLAEIDELQAAGVDVASRLAISG
                     NCPLILEYHMALDKAREVRRGDSKIGTTGRGIGPAYEDKVARRAVRAQDLFDAVRFGD
                     GVREALDFHNFVLEHYFKAPSVDASKVIDDTLALAGRLKPLITDVSHRLNSVMASGHR
                     LLFEGAQGTLLDVDHGTYPYVTSSNTVAGAAAAGAGVAPQRLDYVLGITKAYSTRVGS
                     GPFPTELSDATGEYLRDRGQEFGSVTGRPRRCGWFDAAGLRRAVQLNGTTGLCITKLD
                     VLDGLPVVRLGVGYRFDGREVDILPYGADAVGRCEPIYEEFAGWSDSTFGLRSWDELP
                     VNARRYLERLADVLGVPIDLVSTGPDREQTIVLRHPFR"
     gene            complement(3552..>4505)
                     /locus_tag="VLN25_03535"
     CDS             complement(3552..>4505)
                     /locus_tag="VLN25_03535"
                     /inference="COORDINATES: protein motif:HMM:NF008935.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HSQ09675.1"
                     /translation="MLGLRADTTPQVARIDAHILNRSGVARLCYAGSVLHARPLHPLA
                     VREPLQVGAELYGAVGSAADSEVLELAVASLRVAGLGRISIDLGHTGVVRGLLDVTRQ
                     SAEVEDEILSALSTKDMPALQQTVSHLPAPVRDGLLALGRLSGGLDTLEQARRLLPAE
                     PGIRSALDHLEALSSRCGADVVSVDLADLHGYRYHNGVTFAVHAPELPGAVLRGGRYD
                     GIGKAFGRARPAVGFSIYLRDLVGLLGTDTPRAILAPAGDDAGLREAIRTLRVAGEIV
                     VQRFAEETGGADPGDFVFDRELQRADSVWQVAARNSAPLVK"
BASE COUNT          786 a         1507 c         1431 g          781 t
ORIGIN      
        1 cttctctatc cgtccgttga cgtcgcagac gatgtaagtc tcggttgtct cgaggtcgat
       61 cgctccgcgc gcctcgcgcg ccgatgcaag cgtctcgaag agcgagtaca ggttctgcaa
      121 atgcggcagc agatcgacga ggcgatgcgc ggagcggctg ccgggcaccg acagcgcctc
      181 ccacacctcg tcataggtaa ggcgcccgtg cgagtgcatg acggccggat agaactggta
      241 cgcatcgagc gcaccctggg cgctgatcac gcagtcgcag accatcacga ggcgatcgac
      301 cgacggattc agcgagcaca gcccgttgga cagcttctcc ggcagcatcg gtatgacccg
      361 gcgcgggaag tacaccgacg tcgatcgatt ctgggcctcg gtgtcgagcg cgtcgcccga
      421 cttcacgtag tgactgacat cggcgatggc gacgatcagc cgccacccct tcttgcggcc
      481 cgcgatcggc tcgcagtaca ccgcgtcgtc gaagtcgcgt gcgtcctcgc cgtcgatcgt
      541 gaccagcggc acgtcgcgca gatcgacacg gccacgaagg tcgcgcggtt gcacgccgtc
      601 cggcaggctg tccgccagcg cgagggcctc ggcgggaaac tcgtggggca ccgagaactt
      661 gcgcacggca atctcgattt ccatgccagg gtcgtcgatg gcgcccagca cctggatcac
      721 gcgcccgacc ggctgcacgt agcgctgtgg ttgctgcagg atctcgacca cgacgacctg
      781 gccgggctct gccgccaggg tcgcacccgg tggcacgagg atgtcatgct ggatgcgctg
      841 atcctccggc acgacgatgg tcacacctcg ctcgttgagc aagcggccga cgagcttgtt
      901 ggtgcggctt tcgatgacct cgacgatctc gccttcgggg cggccgcggc ggtcggtgcc
      961 ggacacgcgc gccagcacgc ggtcgccatg cagcaccttg ctcatttcgt gctcggacag
     1021 gacgaggtcc tggcccgcgt cgtcgcggat caggaagcca aagccgtcgc ggtggccctg
     1081 cacctggcct gaaataaggt tggcgcgggc ggcgaccagc agcaggccgg cgcggttgcg
     1141 cacgacgcgc ccggcgcgct ccagttcctc gagctcggtg ttgaaggcgg gacgagcatc
     1201 gtccgcgacg tgcaggcggt ctgcgagttc ctgcggagtc aggggcgccc gcgccgcggt
     1261 cagcgcctgc aatagtgcat cgccgtcagt gggtgtggat ttggaagtca atcagttcct
     1321 tgttgtagtg taggttgcac gtgctccggc tcgaggtcga gccagcgtag cgcatttgac
     1381 atggcgttca ccgccgctag aatcgcgcgc tcacctgtgc ccaggtggcg gaattggtag
     1441 acgcactagt ttcaggtact agcgccgcaa ggtgtggagg ttcgagtcct ctcctgggca
     1501 ccatcttcga aaggccggca tcgccggcct tttgcgttct tgcgcccagg aagcgagcac
     1561 ctgcgtgctt cgcgtgagga cgagaatggc gccgcatgcc aagcggcgcc ggggtcgcgt
     1621 accgtgaccg agtcctctcc tgggcaccag agtattcgaa gggccggcat cgccggcctt
     1681 ttgcattctc agatcgatac tcagcccgat gccgggcctc gtagccgcgc cgcgagattc
     1741 tgcggtccca tcgcgtcgta gacctcaaag ggctgatgaa tccacggatt ggtcggcaag
     1801 tattcgacgt ggtagtccgg attgaacgtc gagcaggcct tgtgccagat caccgccgtg
     1861 cggacctcgt cgagctcggg aaagcgttcc ttgaggtgtt cgaccacccg gcccaacgtg
     1921 acgcccgagt cgaccaggtc gtcgaccagc agcagtcgac ccttcagtgt tccctgggtc
     1981 atcgtgatgt acttgccgat atcgagctcg ccctgcgagc gacccgctgc ctcccggtac
     2041 gaactggcag caagtatcgc caacggcatg tcgtagatgc gcgaaatgat gtcgccgacc
     2101 cgcagacctc ctcgggcgag acacagcagt gaatcgaact gccatcccga ctcgtgtatc
     2161 gcgagactca gccgctcgat gagccggtgg tactcgtccc atgtgacgta gagatccttc
     2221 acgacacttc gatcaattcc gcaggccgcg tcagcggaac gggtgccgca acacgatcgt
     2281 ctgctcgcgg tccggtccgg tggagaccag atcgatcggt acgccgagaa catcggcaag
     2341 gcgctcgagg taacgccgcg cattgaccgg cagttcatcc catgaacgca gaccgaaggt
     2401 cgagtcggac catccggcga actcttcgta gatcggttca cagcgtccga ccgcgtccgc
     2461 tccatagggc aggatgtcga cctcgcgacc gtcgaagcgg tagccgacac cgagcctgac
     2521 caccggcaat ccatcgagga catccagctt cgtgatgcac agtccggtgg tgccgttgag
     2581 ctgcaccgcc cgacgcaatc cggcggcgtc gaaccagcca caacgacgcg gccggcctgt
     2641 taccgagccg aactcctgcc cgcgatcgcg caggtattca ccggtggcgt ccgacagttc
     2701 ggtcgggaac ggtcccgatc ccacccgggt cgagtaggcc ttcgtgatgc cgagcacgta
     2761 gtcgagccgt tggggtgcca cacctgcgcc ggcagccgcg gcccctgcga cagtgttgct
     2821 cgacgtgaca tacggatagg tcccgtggtc gacgtcgagc agcgtccctt gcgcgccttc
     2881 gaacagcagt cgatggccac tggccatcac gctgttgagc cggtgcgaca catccgtgat
     2941 caacggcttc agccgacccg ccagtgcgag cgtgtcatcg atcaccttgg acgcatcgac
     3001 cgaaggcgcc ttgaaatagt gttcaaggac gaagttgtgg aagtcgagcg cctcgcgtac
     3061 accgtcgcca aaacgaacgg catcgaagag atcctgcgcg cgaacggccc ggcgcgccac
     3121 cttgtcttcg taggcgggtc cgatcccgcg tccggtcgta ccgatcttcg aatcaccgcg
     3181 ccggacctcg cgcgccttgt cgagcgccat gtggtactcg aggatcaacg ggcaattgcc
     3241 gctgatggca agccgtgacg caacgtcgac acccgcagcc tgaagctcgt cgatttccgc
     3301 cagcagcgcg ggaggcgaca gcacgacgcc gttgccgatg tagcaggctg ttttcggtcg
     3361 caggatgccc gacggtatca accgaagaat ggtcttcttg cccgcgatca ccagcgtgtg
     3421 gccggcgttg tgtccaccct ggaagcggac gacgccatcg acgtcctcgg tgagccagtc
     3481 gacgatcttg cctttgccct cgtcgcccca ttgggtgccg acgacgacca cgttctttgc
     3541 catgccgctt cctatttcac gaggggcgca ctgttgcgtg cagccacctg ccacactgaa
     3601 tccgcacgtt gcagctcgcg gtcaaaaacg aagtcaccgg ggtccgcgcc ccccgtctcc
     3661 tcggcgaacc gctgcaccac gatctcgccg gcgactctca gcgtgcgaat cgcctcgcgc
     3721 agtccggcat cgtcgccggc cggcgccagt atcgcgcgcg gagtatctgt accgagcaac
     3781 ccgaccagat cgcgcaggta gatcgaaaac cccaccgcgg gccgtgcacg gccgaaggcc
     3841 ttgccgatgc cgtcgtaccg tccgcccctc aacaccgcgc ccggcagctc cggtgcatgc
     3901 acggcgaaag tcaccccgtt gtggtaccga tagccgtgca ggtcggccag gtccacggac
     3961 accacatcgg caccgcaacg ggaagacagt gcctcgagat ggtcgagcgc tgaccggatt
     4021 cccggttcgg ccggaagcaa tctgcgtgct tgttccagtg tgtcgagccc gccgctgagt
     4081 cgtcccaacg cgagcaggcc atcgcgcacc ggtgccggta gatgactgac cgtctgctgc
     4141 agcgccggca tgtcctttgt actgagggcc gacagaatct cgtcttccac ctctgccgac
     4201 tggcgcgtca cgtcgagcag accgcgcacg acgccggtgt ggcccagatc gatactgatc
     4261 cggccgagtc ctgcaacgcg cagcgaggcg acggccagtt cgaggacttc ggagtcggcg
     4321 gcgctgccca ccgctccgta gagttccgcc ccgacttgca gcggctcccg cactgccaat
     4381 ggatgcaggg gccgcgcatg tagcacactg cccgcgtagc acaggcgcgc gacaccggac
     4441 cgattgagta tgtgcgcgtc gatccgcgct acctgcggag tcgtgtcggc acgcagcccc
     4501 agcat
//
LOCUS       DATIRT010000147         3425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_903150, whole genome shotgun sequence.
ACCESSION   DATIRT010000147 DATIRT010000000
VERSION     DATIRT010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903150"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VLN25_03540"
     CDS             <1..435
                     /locus_tag="VLN25_03540"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HSQ09676.1"
                     /translation="LERLTMYLQDVDNVFDLVYTRWKDRGVERVLSYGDVFHQNEVEQ
                     STYNFEASDADFLLGQFGLFEAEARRLMDAQLALPAYEMVLKCSHTFNLLDARGAISV
                     TERAGYIGRIRNLARAVAQSYYDSRERLGFPMAVDAAAQAAV"
     gene            435..2564
                     /gene="glyS"
                     /locus_tag="VLN25_03545"
     CDS             435..2564
                     /gene="glyS"
                     /locus_tag="VLN25_03545"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HSQ09677.1"
                     /translation="MTTASLLVELHTEELPPRALKSLSEAFATGIEANLYSRQFLTPE
                     SRVTAFGAPRRLAVHITSVKARSADQPFRQKLMPLAVARDAARNWTQAFLKKLEGLGR
                     GHLAQVPIGSSGGADSLVVENDGKAEAIFLHGITAGQNLHVGLQAALDETIAGLPIPK
                     VMSYQLADGSTTVQFVRPAHRLVAMHGHQVVPVQALGLLAGTKTLGHRFMCKGDVVIH
                     AADTYVEQMEFEGKVVPSFAARRQRIEEQLAREAARIGATVVAPEDLLDEVTALVEWP
                     VVYESGFEPEFLEVPPECLILTMQQNQKYFALRDADGALLNRFLLVSNLDTKDPEAIV
                     AGNARVVRARLADAKFFYDQDRKQTLESRVSGLANVVYHNKLGSQLARVERVTGIAVG
                     IARQLGLDVTHVERAARLAKADLRTLMVGEFPELQGIMGEYYARHDGESDDVAVAIRE
                     HYQPRFAGDAVPTSTAGICVALADKLETLAGLFGIGEKPTGERDPYALRRHALGVLRM
                     LKEQALPLDLGALVDAALAAFPAGVLKDSNARSGLVAFFSDRLAGLLRDEGYGAHEID
                     AVLAEGSLRIDQVPKRLEAVRAFMELPEAISLAAANKRIGNILKKSDIVPEGFDRALL
                     LEPAEQALGNAFVDLQPEAEALYARGDYTAMLRSLAPLKLPVDRFFDEVMVNVDDARL
                     RANRLGLLRGLQATMNRVADISKLTAG"
     gene            2804..>3425
                     /locus_tag="VLN25_03550"
     CDS             2804..>3425
                     /locus_tag="VLN25_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HSQ09678.1"
                     /translation="MLVLRSTLYLLYLIVVVIPWAFVVLSGWLFPVHTRYYLCACYTK
                     MAIWGARVICGIEWKVEGWDNLPNGPAILLPKHQSSWETLWLPSVMPRDLTFVYKREL
                     NWLPFFGWGLASEQMISIDRRKGQDAFEQVVEQGIDRLGRGWWIVIFPEGTRTRPGST
                     RRFKTGGARLAVRTGAFAVPIAVNSGELWPRNAFIRRPGLITVSIGP"
BASE COUNT          560 a         1098 c         1172 g          595 t
ORIGIN      
        1 ctcgagcggc tcacgatgta cctgcaggat gtcgacaacg tgtttgatct cgtctacacg
       61 cgctggaagg accgcggcgt ggagcgcgtg ctgagctacg gcgacgtgtt ccaccagaac
      121 gaggtcgagc agagcaccta caacttcgag gcgagcgatg cggacttcct gctgggacag
      181 ttcggactat tcgaggccga ggcccgtcgc ctgatggacg cgcagctggc gctgcctgcc
      241 tacgaaatgg tgctgaagtg cagccacacc ttcaacctgc tcgatgcgcg cggggcgatc
      301 agcgtgaccg agcgcgctgg ctacatcggc cgcattcgca acctggcgcg ggccgtggcc
      361 cagagttact acgattcgcg cgaacgtctc ggctttccga tggccgttga tgcggccgca
      421 caggcggcgg tgtgatgacg accgcgtcgc tgctcgtcga actgcacacg gaggaactgc
      481 cgccgagggc gctcaagtca ctgtcggaag cgttcgccac cggcatcgag gccaacctct
      541 acagccggca gttcctgacg cccgaaagcc gcgtcaccgc gttcggtgcg ccgcgccggc
      601 tggcggtgca catcacctcg gtgaaggctc ggtcggcgga ccagccgttc cggcagaagc
      661 tgatgccgct ggcggtggcg cgcgatgcgg cgcgcaactg gacacaggcc tttctcaaga
      721 aactcgaggg actcggacgc ggccacctgg cacaggtgcc catcggcagc agcggcggcg
      781 ccgattcact ggtggtcgag aacgacggca aggcggaggc gatcttcctg cacggcatca
      841 ccgcaggcca gaatttgcac gtgggcctgc aggcggcact cgacgaaacg atcgcgggtc
      901 tgccgattcc aaaggtcatg agctaccagc ttgccgacgg cagcacgacg gttcagttcg
      961 tgcggccagc gcaccggctg gtggcgatgc atggccacca ggtcgtgccg gtgcaggccc
     1021 tcggtctgtt ggccggcacc aagaccctcg gacaccgttt catgtgcaag ggcgatgtag
     1081 tgatccatgc ggccgatacc tacgtcgaac agatggagtt cgaaggcaag gtcgtgccgt
     1141 cgttcgcggc gcggcgccag cgcatcgagg agcaactggc gcgtgaggcg gcgcgcatcg
     1201 gcgcaaccgt cgtggcgccc gaggacctgc tcgatgaagt gacggcgctg gtcgagtggc
     1261 cggtggtcta cgaatcgggt ttcgagcccg agtttctcga ggttccaccg gagtgcctga
     1321 tcctcacgat gcagcagaac cagaagtact tcgcgctgcg cgatgcggac ggtgccttgc
     1381 tcaaccgctt cctgctggtc tcgaacctcg acaccaagga tccggaggcc atcgttgccg
     1441 gcaacgcccg tgtggtgcgt gcccgcctgg ctgacgccaa gtttttctac gaccaggatc
     1501 gcaagcagac gctggagagc cgggtgagcg gcctggcgaa cgtcgtgtat cacaacaagc
     1561 tcggctcgca gctcgcgcgc gtcgagcgcg tcaccggcat cgcggtcggc atcgcgcggc
     1621 aactcggact ggacgtcacg catgtcgagc gtgccgcgcg cctggcgaag gccgacctgc
     1681 gaacgctgat ggtcggtgag tttccggaac tgcagggcat catgggcgag tactacgcgc
     1741 gccacgacgg cgagtccgat gacgtggccg tcgcaatccg cgagcactac cagccgcgct
     1801 ttgccggcga tgcggtgccc acaagcacgg cgggtatctg cgttgcgctg gcggacaagc
     1861 tcgagacgct agccggcctc ttcggcatcg gtgaaaaacc gaccggcgag cgcgatccgt
     1921 atgcgttgcg ccgccacgcg ctcggcgtgc tgcgcatgct gaaggagcag gccctgccgc
     1981 tcgatctcgg tgcgctggtg gatgcagcgc tcgccgcctt cccggcgggc gtgctgaagg
     2041 acagcaacgc ccgctccggc ctggtcgcat ttttctccga tcggctcgcc gggctgctcc
     2101 gcgatgaagg ctacggtgcc cacgagatcg atgccgtgct tgccgagggt tcactgcgca
     2161 tcgaccaggt gcccaagcgg ctcgaagccg tgcgcgcctt catggagctt ccggaggcga
     2221 tcagcctggc ggcggccaac aagcggatcg gcaatatcct gaagaagtcc gatatcgtgc
     2281 ccgaaggctt tgaccgggct cttctgctcg agccggccga acaggcgctc ggcaacgctt
     2341 tcgttgacct gcagccggag gcggaggcgc tctacgcgcg gggcgattac acggccatgc
     2401 tgcgctcgct tgcgccactg aagttgccgg tcgaccgctt cttcgacgaa gtcatggtca
     2461 atgtcgacga tgctcgcctg cgcgccaacc gcctcgggct gctcagggga ctgcaggcca
     2521 cgatgaatcg cgtggctgac atctccaagc tcacggcggg ctaggttaat gacccccacg
     2581 ctcactgcgt tcactgcccc ccgagggggc ggacagtcgg cttgggggcg gcccggcgcc
     2641 gacttgatga cccccacgct cactgcgttc gctggaccac ggttcggctt gcaggccgaa
     2701 ccgctgcgcg aggcgtgcat caggccgcag ggccagctgc acgtccgcgc gcagcccctt
     2761 gggggccgcc agtcggcttg ggagcggccc ggcgccgact gacatgctgg tcctgcgctc
     2821 gacgctgtat ctgctctacc tgatcgtcgt tgtcatcccg tgggccttcg tcgtgctgtc
     2881 gggttggctg ttcccggtgc atacgcgcta ctacctgtgc gcgtgctaca cgaaaatggc
     2941 gatctggggc gcgcgcgtca tctgcggcat tgagtggaaa gtcgagggct gggacaacct
     3001 gcccaatggg ccggcgatcc tgctgccgaa gcaccagtcc tcatgggaga cgctgtggct
     3061 gccgtcggtg atgccccgtg acctgacctt tgtttacaag cgcgaactga actggttgcc
     3121 gtttttcggt tgggggctgg cctcggagca gatgatcagc atcgatcgcc gcaaggggca
     3181 ggacgccttc gagcaggtgg tcgagcaggg tatcgatcgc ctcggccgtg gctggtggat
     3241 cgtgatcttt cccgagggca cgcgcacgcg gcccgggtcg acgcggcgct tcaagacagg
     3301 tggcgcgcgg ctcgcggtac gcacgggagc gtttgccgta ccgatcgcgg tgaactctgg
     3361 cgaactgtgg ccgcggaacg cattcatcag gcggccagga ctgatcacgg tgtcgatcgg
     3421 ccctc
//
LOCUS       DATIRT010000148         2258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1689895, whole genome shotgun sequence.
ACCESSION   DATIRT010000148 DATIRT010000000
VERSION     DATIRT010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1689895"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..>2258
                     /gene="ileS"
                     /locus_tag="VLN25_03555"
     CDS             193..>2258
                     /gene="ileS"
                     /locus_tag="VLN25_03555"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HSQ09679.1"
                     /translation="MRGDLAKREPQWIREWDEQKVYEQIRAASVGRPKWVLHDGPPYA
                     NNDIHMGTAINKVLKDIVVKSRVMAGFDAPYVPGWDCHGMPIEIQIEKKYGKHLPPRE
                     VIAKSRAWATEQIDRQRKDFKRLGVLGAWDRPYTTMNFRAEANELRALAKIIAKGFVF
                     RGLKPVNWCFDCGSALAEAEVEYQDKVDLAVDVAFPFRDDERGKLAAAFGLAGLPDKP
                     VAAAIWTTTPWTIPANQALNLHPEISYDLVDTGKGLLLLASERRDACLASYGLTGTTL
                     AQTVGEKLALIRFRHPFYERDAPVYLGDYVTLDTGTGIVHSSPAYGVEDFISCKAHGM
                     TDDEILNPVQGDGVYASSLPLFGGMKIWDANSKVVDVMREHGALLKVEKFTHSYMHCW
                     RHRTPVIYRATSQWFAGMDTLPKSGGKTLRELALTGIESTQFYPAWGKARLQGMIANR
                     PDWTLSRQRQWGTPMAFFVHKSTGELHPRTLELIEQVAQRVEKSGIEAWQDLDPQELL
                     GAHGPDSAEHYAKNKDTLDVWFDSGTTHETVLRGSHADESQFPADMYLEGSDQHRGWF
                     HSSLLTSCMINGVPPYKALLTHGFVVDASGHKMSKSKGNVVAPQQVMDELGADILRLW
                     VASTDYSGELSISKEILKRVVESYRRIRNTLRFLLANLADFDAARDFLPIAEWLEIDR
                     YAWVMT"
BASE COUNT          440 a          747 c          708 g          363 t
ORIGIN      
        1 cggcgacgag ccgggcggca ccacgctaca ctcgcgctcc gcttttcatt gacacctctt
       61 gcagagcccg agggggccaa ggagtgccgc accatggctg acgccgacgc caagcgcatc
      121 gacaaaagca agcccgcgtc cgatcccgcg acgcgcaagt acccgctcaa cctgcccgat
      181 acgccgttcc cgatgcgcgg cgatctcgcc aaacgcgagc cgcagtggat ccgcgaatgg
      241 gatgagcaaa aggtctacga gcagatacgc gccgcttccg tgggtcggcc gaagtgggtc
      301 ctgcacgacg gtccgccgta cgcgaacaac gacatccaca tggggaccgc gatcaacaag
      361 gtcctgaagg acatcgtcgt caagagccgc gtgatggccg gcttcgatgc gccttacgtt
      421 ccgggatggg actgtcacgg catgccgatc gagatccaga tcgagaagaa atacggcaag
      481 cacctgccgc cgcgcgaagt gatcgcgaaa tcgcgcgcgt gggcgacgga gcagatcgac
      541 cggcagcgca aggacttcaa gcggctcggc gtcctcggcg cctgggatcg gccgtacaca
      601 acgatgaact tcagagccga ggccaacgaa ctccgcgcac tggcgaagat catcgccaag
      661 ggcttcgtgt tccggggcct gaagcccgtt aactggtgct ttgactgcgg ctccgccctc
      721 gccgaggccg aggtcgaata ccaggacaag gtggatctcg ccgtcgacgt tgccttcccc
      781 tttcgagatg acgagcgcgg caaacttgct gccgcattcg gacttgcggg actgcccgac
      841 aagcccgtcg ccgccgccat ctggaccacg acaccctgga cgatcccggc caaccaggcc
      901 ctcaacctcc atccggagat cagctacgac ctggtcgata ccggcaaggg cttactgctg
      961 ctcgccagcg agcggcgcga tgcgtgcctc gcctcgtatg gactgacagg cacgacgctg
     1021 gcacaaaccg tcggcgaaaa actcgccctg atccgcttcc gccacccgtt ctacgagcgc
     1081 gacgcgccgg tctacctcgg cgattacgtg acactggaca ccggcaccgg catcgtgcac
     1141 tcgtcacccg cctacggcgt cgaggacttc atttcgtgca aggcgcacgg catgaccgac
     1201 gacgagatcc tgaacccggt ccagggagat ggagtctatg cgtcgtcgtt gccgctcttc
     1261 ggcggcatga agatctggga tgcgaactcc aaggtcgtag acgtcatgcg ggagcacggt
     1321 gcactgctga aggtcgagaa gttcacacac agctacatgc actgctggcg gcatcgcaca
     1381 ccggtgatct accgcgcgac ctcgcagtgg ttcgccggca tggacacgct gccgaagtcg
     1441 ggcggaaaga cgctgcggga gctcgcactc accggcatcg agtccacgca gttctatccg
     1501 gcgtggggca aggcgcgttt gcagggcatg atcgctaacc gcccggactg gaccctgtcg
     1561 cgacagcgcc agtggggtac gccgatggcg ttcttcgtac ataagtcgac cggcgagctg
     1621 cacccgcgca cgctcgagct gatcgagcag gtcgcgcagc gcgtcgagaa gtcgggcatc
     1681 gaagcctggc aggacctcga tccgcaggaa ttgctgggcg cgcatggacc cgactcggcc
     1741 gagcactacg cgaagaacaa ggacacgctc gacgtctggt tcgactccgg caccacgcac
     1801 gagactgtgc tgcgcggttc gcacgccgac gaatcacagt tcccggccga catgtacctg
     1861 gaaggctcgg accagcaccg cggctggttc cattcgtcgc tgctcacctc gtgcatgatc
     1921 aacggcgtgc cgccgtacaa ggcgctgctg acgcacggct tcgtggtcga cgcctcgggc
     1981 cacaagatga gcaagtccaa gggcaacgtc gtggcgccgc agcaggtgat ggacgagctc
     2041 ggcgccgaca tcctgcgcct gtgggtggcc tccaccgatt actcggggga gctctcgatc
     2101 tcgaaggaga ttctgaagcg cgtcgtggaa tcgtatcgcc gcatccgcaa cacgctccgc
     2161 ttcctgctcg cgaacctcgc ggacttcgat gccgcgcgcg attttctgcc gatcgcggaa
     2221 tggctcgaga tcgaccgcta tgcgtgggtg atgacgca
//
LOCUS       DATIRT010000149         1979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1296692, whole genome shotgun sequence.
ACCESSION   DATIRT010000149 DATIRT010000000
VERSION     DATIRT010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296692"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..682
                     /locus_tag="VLN25_03560"
     CDS             200..682
                     /locus_tag="VLN25_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HSQ09680.1"
                     /translation="MTANTSTQTLLARHPFTHGMGGPAIEKLASLARNVSFARNEVIF
                     REGDDIHDFFLVVSGRVALEIVSEGAAHRVHTLSAGDELGWSAVLMGQGKYFQARALE
                     QAGALAFDGGELLALCESDPAFGYKLMHRLLGVVAERLQATRLQLLDTYLPAAHKAGA
                     "
     gene            complement(792..>1979)
                     /locus_tag="VLN25_03565"
     CDS             complement(792..>1979)
                     /locus_tag="VLN25_03565"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HSQ09681.1"
                     /translation="DRSGLIATPYRDYRGTQVIGAWQWLPAYDVGVAAEISESEAFST
                     LRYITISFAVIGGITALSLVAAFMSAFLLARLSRQFGRVQRLGAYTLDRKVSEGGMAT
                     IYLARHALLKRPTAIKILKKHVATDEFVHRFEREVQLASQLMHPNTVEIYDFGRTREG
                     QPYYVMEFLDGVTLAELVEHSGRVPPGRAIHILRQVAAALREAHMHGMIHRDVKPENV
                     MLCRRGEDDVVKLLDFGLVKNLERADTRDITKQLKIVGTPRYMAPERLVNPSDVDARS
                     DIYALGAVGYYLLTGKVIFDGDDSLQISNQVLHAMAPRVRDAGVEVPEGLDNLIAACL
                     EKDRNRRPRNAEAVVEALDRLASRLAWTQSDAVAWWTEYRKTRRPAAGMAASDGRRKT
                     TAA"
BASE COUNT          332 a          679 c          634 g          334 t
ORIGIN      
        1 tgctagaacg caccacggcc atgccagttc cgcccctccc aagacgccgc gctatatcct
       61 gcacgcaaac gcgtccggcg accatagtgg gctctgctca tcacggcttg acaacggtca
      121 acgccccgcc ttgggggcgg gcgcagccta tgccctcaat ccgggcgacg acccggcaca
      181 gcttgtggaa ggagccctga tgactgccaa cacgtcgacc cagaccctgc tggctcgcca
      241 tccgttcacg cacggcatgg gaggaccggc catcgagaag cttgcgtcac ttgcgcgcaa
      301 cgtcagcttc gcacgcaacg aggtgatttt tcgcgagggc gacgacatcc acgacttctt
      361 cctggtcgtg tccggccgcg tggcgctcga gatcgtttcc gagggcgcgg cgcaccgcgt
      421 gcacacgctg agcgccggag acgaactcgg gtggtctgcc gtgctgatgg gccagggcaa
      481 atacttccag gcccgcgcgc tggagcaggc gggagcactc gcctttgatg gcggcgagct
      541 actcgcgctg tgcgaaagcg accccgcgtt tggctacaag ctgatgcatc gcctgctcgg
      601 cgtcgtcgcc gaacgtctgc aggccacgcg ccttcaattg ctcgacacgt acttgccggc
      661 agcccacaaa gccggcgcct gacacgcgca cggtgagcgt cgctggcatc acgattcgcg
      721 ggggcactgc gcgcagacgc gcctgacgcc cccgcagcaa tgcaagacag gcggagtcgc
      781 ggcagttccg atcaggccgc cgtcgtctta cgccggccgt cagaagcagc catgccggcg
      841 gccgggcgcc gagtctttcg atactccgtc caccaggcca ccgcgtcgga ctgcgtccac
      901 gccagacggc tggcaagccg atccagggcc tcgaccaccg cctcggcgtt gcgcgggcga
      961 cggttgcggt ccttctcaag gcaggcggca ataaggttgt cgagcccctc cggcacttcg
     1021 acccccgcat ctcgcacccg cggcgccatc gcgtgcagga cctggttgct gatctgcagg
     1081 ctgtcgtcac cgtcgaagat gaccttgccg gtcagcaggt agtagccgac cgcgccgagc
     1141 gcgtagatgt ccgagcgcgc atcgacatcg gagggattga ccaggcgctc cggagccatg
     1201 tagcgcggcg tgccgacgat cttgagctgt ttggtgatgt cgcgtgtgtc agcgcgctcg
     1261 aggtttttca ccaggccgaa gtcgagcagc ttgaccacgt cgtcctcccc gcgccggcac
     1321 agcatcacgt tttccggctt cacgtcgcgg tggatcatgc cgtgcatgtg cgcctcgcgc
     1381 agcgcggccg cgacctggcg caggatgtgg atcgcccggc ccggaggcac ccgtcccgag
     1441 tgctccacca attcggccag cgtcacgcca tcgaggaatt ccatcacgta gtacggctgg
     1501 ccctcgcgcg tgcgcccgaa gtcgtagatc tcgaccgtgt tggggtgcat cagctggctc
     1561 gccagttgca cctcgcgctc gaaccggtgc acgaactcgt cggtggcgac atgcttcttc
     1621 aggatcttga tcgcggtcgg acgcttcaag agcgcatgcc gcgccaggta gatcgtggcc
     1681 atcccccctt cgctgacctt gcggtcgagc gtgtatgcgc cgaggcgctg cacgcgaccg
     1741 aactgccggc tgagccgtgc cagcaggaac gccgacatga acgcagccac cagagacagg
     1801 gcggtaatgc cgccgatgac ggcgaagctg atcgttatat agcgcagcgt cgagaacgcc
     1861 tcgctctcgg aaatttcggc ggccaccccg acatcgtagg caggcagcca ttgccaggcg
     1921 ccgattacct gggtgccgcg atagtcacga tagggtgtcg cgatgagccc gctgcggtc
//
LOCUS       DATIRT010000150         1629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1591706, whole genome shotgun sequence.
ACCESSION   DATIRT010000150 DATIRT010000000
VERSION     DATIRT010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591706"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(475..909)
                     /locus_tag="VLN25_03570"
     CDS             complement(475..909)
                     /locus_tag="VLN25_03570"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HSQ09682.1"
                     /translation="MLIRFIHRLGRWIPVCLIVAATGSVVMAEAAARADEGKVEAIEI
                     IYSAPDNKPVNKGTVIGGIAGGVIGHQIGSGRGKTAATVAGTIGGAVIGNEIEKKQQA
                     TPRYRISVRLDSGSSMIVEETRDLNLRVGDRVRIEDGHISRI"
     gene            complement(997..>1629)
                     /locus_tag="VLN25_03575"
     CDS             complement(997..>1629)
                     /locus_tag="VLN25_03575"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HSQ09683.1"
                     /translation="VGAPYGISETLTVGYVSARRTARSEPGDPASVEVFQTDAAIAPG
                     NSGGPMFNMQGEVIGIVSRIVTRSGGSEGIGFAVTSNAAKEILLDAPPIWTGVSVVPL
                     SGELARALNLPQPAGLLVMHAAEDSPGAQMGLRAGTTPAVVGGVNLTLGGDIILAVAG
                     MPIEANVSNYRSIRDRLRRSQPGEQVPIVVLRSGRIVELSGANMRTTRAN"
BASE COUNT          295 a          517 c          505 g          312 t
ORIGIN      
        1 ggaaaggcgg tgacctgagt ccggccacgc ttggtaaggg cgtagatctt gaagaacccc
       61 gagcggattg cgtagagcga gtcgaacggg tcgccggcgc ggaacagatg ttctccgctg
      121 tggacacgtt ttctcgtaag aacgagttcg acgtcgacag gcatcgagtc gtccgattgc
      181 atagctccga gcaaacagtg cttgcgtgcg cgacaagtcg agcacagcgt cggcgcagcc
      241 gccggcacgg atcgcgccgg cgtccaactc gggagcgaac gggcaagcgc aacgggctgc
      301 gaagccggac tgacttgatc acaagttgac tgcatgaaag cctcctaggt ccggctaact
      361 cgggcggatg cgctgccgat ccccgcacgg tggatgcact gcctcgcgga cgacacaaca
      421 tcgttgaccg aagcgaatct tccgatcatt ccggtcatgg ccacgagccc ctgcctagat
      481 ccgcgagatg tgaccgtcct caatccgcac acggtccccc acccgcaggt tgagatcgcg
      541 cgtttcttcg acgatcatcg agctgcccga atcgagtcgc acggagatgc ggtagcgcgg
      601 cgtcgcttgc tgcttcttct cgatttcgtt tcctataacc gccccgccga ttgtgccggc
      661 gacggttgcc gccgttttcc cgcggccgct tccgatctgg tggccgatca caccaccggc
      721 gataccgccg atcacggttc ctttattgac aggcttgttg tcgggcgcgc tatagatgat
      781 ctcgatggcc tcgactttgc cttcgtcagc gcgggcggca gcttcggcca tcacaacgct
      841 gccggtggca gcgacgatca ggcaaaccgg tatccaacga cccaaccggt gaatgaatcg
      901 aatcagcatg tttgttctcc tttgcttgaa gttgatgcaa tttgtcgtta tccacacgcg
      961 gctcttctac gccgagggct tggcggacag taagagtcag ttcgcgcggg tcgtgcgcat
     1021 gttcgcgccg ctaagctcga cgatccgccc agagcgcagc acaacgatgg gaacctgctc
     1081 ccctggctgc gatcgtcgca atcgatcgcg gatactgcgg tagttgctga cgttcgcttc
     1141 tatcggcatc ccggcaaccg ccaaaatgat gtcgccgccg agcgtcaggt tcactccgcc
     1201 caccaccgcg ggcgtagtgc ctgccctgag gcccatttgc gccccgggtg agtcttcggc
     1261 tgcgtgcatc accagcaatc cggcgggttg cgggagattg agagcccgag ccagttcccc
     1321 ggacagcggc acgacggaaa cgccggtcca gatgggtggc gcatcgagca ggatttcctt
     1381 cgcggcgttg gaagttacgg caaagccgat gccttcggag cctccggagc gagtgacgat
     1441 gcggctgacg atgccgatca cctcgccctg catgttgaac atcggtccgc cggaattgcc
     1501 gggcgcaatc gccgcgtcag tctgaaacac ttccaccgag gcggggtcgc cgggctccga
     1561 gcgagccgtg cgccgcgcgc tgacgtagcc gacggtcagc gtttcgctga tgccgtacgg
     1621 cgcgccgac
//
LOCUS       DATIRT010000151         3090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_903609, whole genome shotgun sequence.
ACCESSION   DATIRT010000151 DATIRT010000000
VERSION     DATIRT010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903609"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VLN25_03580"
     CDS             complement(<1..446)
                     /locus_tag="VLN25_03580"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HSQ09684.1"
                     /translation="MQLLLSLVEHYGLWLVFANVLALQLGLPLPAYPTLILVGAIASR
                     GDFTVPQVIAVAVLASVIADLAWYLAGARIGRRVLRVMCRLSLSPDSCVSQTERIYER
                     WGAPSLTIAKFVPGFAAVATSMAGVMRTRLASCLAFDAIGALLWSGV"
     gene            805..1098
                     /locus_tag="VLN25_03585"
     CDS             805..1098
                     /locus_tag="VLN25_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HSQ09685.1"
                     /translation="MPTYIVLGSFTDQGIRGVKDTTKRADAVREHAKKFGVTAKEFYW
                     TLGAHDMVVILDAPDDAAMSAFGMSVGSAGNVRTQTMRAFSKDEVNSILGKLA"
     gene            complement(1184..1495)
                     /locus_tag="VLN25_03590"
     CDS             complement(1184..1495)
                     /locus_tag="VLN25_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09686.1"
                     /translation="MKSIVTRFFSTVVLGIAFVATAHAEYRCDPAPTWIDQRACEAAG
                     ESPQALRQYVQRMRWFTNLQFSDYVNENTAQGWEAKKREMAARKSSEDGVISVAFSER
                     G"
     gene            complement(1772..>3090)
                     /locus_tag="VLN25_03595"
     CDS             complement(1772..>3090)
                     /locus_tag="VLN25_03595"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HSQ09687.1"
                     /translation="IVTALPTVYFGRDVPANRVTSVDLAPLAADDVDPDTLKIGAKTA
                     LDLTWKEGLDAFTCTECGRCKDACPTYLTGKPLALKWVHDAVKRHLVEHADAIRAHDA
                     AKLPAIIGSAISDDTLWACTTCGYCEVACPIQLEHLPKFYRLRQRQVMMEGEFPHELN
                     RVFAAYESQGNPWGLDAATRGDWAKDLGVPVVTSADEVATLDYLYYVGSASSFDPRGQ
                     KVARAFVKVAQAAGVRLGILGPQEGSTGECVRRLGNEMVGQQLATSLVETLNRLGVKR
                     IVTCDPHAFNALKNELPEFGGHYEVVHHTQAIDLWMRDGRLRTAPTHERVIYHEPCYL
                     GRHNGEYDAPRRVLQAISRGAPLEFDLARDKAMCCGAGGGRMWLEETIGTRINVLRAE
                     QALAKHPVAVATGCPYCAVMVGDGLAQKDDRVPARDIAELVAEALQ"
BASE COUNT          562 a          965 c         1020 g          543 t
ORIGIN      
        1 acgccgctcc aaagcagtgc gccgattgca tcgaacgcta gacaactcgc aaggcgtgtc
       61 cgcatgacgc ccgccatgct tgttgccacc gcggcaaagc cggggacgaa cttggcgatc
      121 gtcagtgacg gtgcacccca acgttcatag atgcgttctg tctggctcac gcagctgtcg
      181 ggcgacagcg agagccggca catcacgcgc agtacgcgac ggccgatcct ggcgcccgcg
      241 agataccagg cgagatcagc gatgacgctt gccaggactg caaccgcgat cacctggggt
      301 acggtgaagt cgccgcgtga cgcgatggcg ccaaccagga tcagtgtcgg gtacgccggc
      361 agcggcaggc cgagctgcaa ggcgaggacg ttggcaaaga ccagccacag accgtagtgc
      421 tcgacgagag aaagcagcag ttgcatcaga caaagtacgt cggcgcgtca tgccgttcca
      481 cggtagcaac ggcggtcgcc tgacgatagt tcgattgcat tccgtgggca aggctggaga
      541 ttgtggggtg acgatcagtc gtgtctggca tcaatggcga cagttggaag gccgcgcgat
      601 cgccactgct ctgtgaggcg tgcaagaaat ccagcgcaat gccgtggcca aggagtatct
      661 tggccgatcc tcgacaggac gaccgccggg acaactgcgc cctgcggctg cctatgccga
      721 tgggcgacgg gaacagacga ggcctgagtc cacgccgcca gcggttttcc aaccaggcct
      781 cgcgaccgac atcaggagac agacatgccg acctacatcg ttttgggcag cttcaccgat
      841 cagggtatcc gcggcgtcaa ggacacgacc aagcgcgctg acgcggtccg ggagcacgcg
      901 aagaagttcg gcgtcacggc caaggagttc tactggaccc tgggtgcaca cgacatggtc
      961 gtgatcttgg atgctccgga cgatgctgcg atgtccgcgt tcggcatgag tgtcggttcc
     1021 gcgggcaacg tgcggacgca gacgatgcgc gcattctcga aggacgaggt caactcgatt
     1081 ctcggcaagc tggcgtagtc gtggcggcgc tgtgccgttc ggggcacgcc tcccgaacgg
     1141 cgcaacgatc ccacggatcc gcagagcgcg acgtaacagt cggtcagccg cgttcgctga
     1201 atgcgacgga gatgacaccg tcctccgatg acttccgtgc ggccatttcg cgcttcttcg
     1261 cttcccatcc ttgcgcggtg ttctcgttga catagtccga gaactgaagg ttcgtgaacc
     1321 aacgcatgcg ttgcacgtac tgacgcagtg cctgagggct ctccccagct gcctcgcagg
     1381 cacgctgatc gatccaggta ggcgccggat cgcaacgata ttcggcgtgc gcggtcgcca
     1441 cgaaggcgat gccgaggaca acggtggaga agaagcgagt gacgatgctc ttcatgtctt
     1501 gactcctttg tgggaaggtc agggggttga tcggatgcac cccgtggagg cgggcatcag
     1561 atgaatcgca cggtaggtcg acaccgcgtc ggcggcgatg ggcaatgcgg cgtcgtcaat
     1621 accgaaaatt cgacaagcgc ggggccagcg tttccgatcg ggatacgtag tcaggcgcca
     1681 gcaccacggc gagcccacgc cgtgggtaac gactgttttt ttgtgaagtg ccgctgccat
     1741 cgacggcagc agccggagga tcggaacctg actactggag cgcctcggcc accagctcgg
     1801 caatatcgcg cgcgggcacg cggtcgtcct tctgtgccag gccgtcccca accatcactg
     1861 cgcaatacgg gcagccggta gccaccgcga ccggatgctt tgccagtgcc tgctcggcgc
     1921 gcagcacgtt gatacgcgtg ccgatcgttt cctcgagcca catacggccg cctcccgccc
     1981 cgcagcacat cgccttgtcg cgggcgaggt cgaattcgag cggcgcaccc ctggagatcg
     2041 cctgcagcac gcggcgcggc gcgtcgtact cgccgttatg ccggccgaga tagcacggct
     2101 cgtggtagat cacgcgctcg tgcgtcggcg cagtcctcag tctgccgtcg cgcatccaaa
     2161 ggtcgatagc ctgtgtgtga tgcacgacct cgtagtgacc gccgaactcc gggagttcgt
     2221 tcttcagcgc gttgaaggca tgcgggtcgc aggtgacgat gcgcttgacg ccgaggcggt
     2281 tcagcgtctc gaccaggctg gtggcgagct gctggccgac catttcgttg ccgagccggc
     2341 gcacgcactc gccggtcgat ccttcctgcg gaccgagtat gccgagtcgc actccggccg
     2401 cctgcgcaac cttcacgaac gcgcgtgcga ccttctggcc gcgcggatcg aacgaggatg
     2461 cggagccgac gtagtagagg tagtcgagcg tcgcgacttc gtcagcactg gtaacgaccg
     2521 gcacgcccag gtccttggcc cagtcgccgc gcgttgcggc gtccagtccc cagggattgc
     2581 cctgtgactc gtaggcggca aagacgcgat tcagttcatg tgggaactcg ccctccatca
     2641 tgacctggcg ctgccgcagc cggtagaact tcggcaagtg ttcgagctgg atcggacagg
     2701 ccacttcgca gtagccgcag gtcgtgcatg cccacagggt gtcgtcgctg atcgcgctgc
     2761 cgatgatcgc cggcagcttc gcggcatcgt gggcgcggat cgcatccgca tgctcgacga
     2821 gatgacgctt caccgcgtcg tgcacccact ttagcgccag cggcttgccg gtcaggtacg
     2881 tggggcaggc gtccttgcag cggccgcact cggtgcaggt gaaggcatcg agtccttcct
     2941 tccacgtgag atcgagcgcg gtcttcgcgc cgatcttcag cgtgtcgggg tcgacgtcgt
     3001 cggccgcgag cggcgcgaga tcgacactcg tcacacggtt ggcgggcacg tcgcgaccga
     3061 agtagaccgt tggcaatgcg gtgacgatgt
//
LOCUS       DATIRT010000152         6936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1002941, whole genome shotgun sequence.
ACCESSION   DATIRT010000152 DATIRT010000000
VERSION     DATIRT010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002941"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VLN25_03600"
     CDS             complement(<1..374)
                     /locus_tag="VLN25_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSQ09688.1"
                     /translation="MAAASKVARRRFDAVIVGAGGSGMRCSLQLAQAGLNVAVLSKVF
                     PTRSHTVAAQGGIGASLGNMTEDNWLWHMFDTVKGSDYLGDQDAIEFMCREASKVVYE
                     LEHFGMPFDRNPDGTIYQRPFGG"
     gene            complement(378..755)
                     /gene="sdhD"
                     /locus_tag="VLN25_03605"
     CDS             complement(378..755)
                     /gene="sdhD"
                     /locus_tag="VLN25_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HSQ09689.1"
                     /translation="MPASPNPAGIGTKRIVVGAHYGVGGFLLQRLTAIVLTVFVVFLL
                     LRLVFGGPLGYDGWAGLFVPLWMKIVTLVALLALFYHAWIGVRDIWMDYVKPTGIRVA
                     LQTLTVLWLVFCAVWSVQILWRV"
     gene            complement(755..1171)
                     /gene="sdhC"
                     /locus_tag="VLN25_03610"
     CDS             complement(755..1171)
                     /gene="sdhC"
                     /locus_tag="VLN25_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HSQ09690.1"
                     /translation="MTTPAFASKPRPEFRNIHVSQLLKYKLPPPGMVSILHRISGAAL
                     FLTLPLILWLFDLSLTSELSYARLADFGAHWLVKLVLVGLAWSFLHHLIAGLRFLLLD
                     LDVGVDKPAARTSALAVFAISLPLTLLAALKIFGAF"
     gene            complement(1243..2007)
                     /locus_tag="VLN25_03615"
     CDS             complement(1243..2007)
                     /locus_tag="VLN25_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HSQ09691.1"
                     /translation="MPSADFPADSPDRVARPAVFSPLYRQIKGLLLRSLDEGEWRPGE
                     LIPSEIELATRFQVSQGTVRKAVDELAAENLLVRRQDKGTFVGTHHEPRAQFRFLRVV
                     PDGGEPAPPQSRFLECRRVRAPADIARLLDLRTGDAAVYLRRLLSFGGDPIVLDEIWL
                     PGGLFKGLTAERLSEYKGPLYGLFETEFGTRMIRAEEQLRAIGAERDAAQLLGVPEKS
                     PLLLVERVSYSYGDRPVEVRRGLCVTDHYHYNNTLS"
     gene            2499..3485
                     /locus_tag="VLN25_03620"
     CDS             2499..3485
                     /locus_tag="VLN25_03620"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HSQ09692.1"
                     /translation="MSKAPVRVAVTGAAGQIGYSLLFRIASGEMLGRDQPVVLQLLEI
                     PDEKAQKALKGVMMELDDCAFPLLAGMEAHSDPTAAFKDTEIALLVGARPRGPGMERK
                     DLLAANAQIFTAQGKALDKAASRNVKVLVVGNPANTNAYIAMKSAPSLPKGNFTAMMR
                     LDHNRALSQLAGKTGKPVASIEKLVVWGNHSPTMYPDIRFATIGGQPAQPMVDPTWYR
                     DTYMPTVGKRGAAIIEARGLSSAASAANAAIDHIRDWVLGTNGRWVTMGVPSDGSYGV
                     PEEIIYGVPCVCAGGKYEVVKGLAIDEFSRGKMDNTLKELVEERDGVKHLLG"
     gene            3522..4409
                     /gene="prpB"
                     /locus_tag="VLN25_03625"
     CDS             3522..4409
                     /gene="prpB"
                     /locus_tag="VLN25_03625"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HSQ09693.1"
                     /translation="MPMSPSAGARFREALQLERPLQIVGTIIANHALMARHVGYRAIY
                     LSGGGVAAGSLGMPDLGINTLDDVLTDTRRITDVCDLPLLVDIDTGFGPSAFNIARTV
                     KSLIKAGAAACHIEDQVGAKRCGHRPGKEIVTTEEMVDRVRAAADAKTDSAFFLIART
                     DAIAVEGIDRAIERAVACAEAGADGIFAEAAKDLATYRRFVDAVKVPVLANITEFGAT
                     PLFTVKELGDAGAAMVLYPLSAFRAMNKAALNVYTTLRRDGTQQGVVETMQTRMELYD
                     VIGYQAYEDRLDQLFAKRK"
     gene            4461..5618
                     /gene="prpC"
                     /locus_tag="VLN25_03630"
     CDS             4461..5618
                     /gene="prpC"
                     /locus_tag="VLN25_03630"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HSQ09694.1"
                     /translation="MNDTPAPEAPKPKKSVALSGVVAGNTALCTVGRTGNDLHYRGYD
                     ILDIADTCEFEEIAHLLVHGKLPNHSELVAYKTKLKALRGLPANVKAALEWLPASSHP
                     MDVMRAGVSALGCVLPEKDDHNVPGAKDIADRLMASLGSMLLYWYHYSINGRRIEVET
                     DDDTIGGHFLHLLHGKPPSQLWVKAMHTSLILYAEHEFNASTFTARVIAGTGSDFYSA
                     IAGAIGALRGPKHGGANEVAFEIQKRYETPDEAEADIRRRVEAKEVVMGFGHPVYTVS
                     DPRNKVIKEVARALSKDIGNTKMFGIAERLETVMWDAKKMFPNLDWFSAVSYHMLGVP
                     TSMFTPLFVISRTSGWSAHIIEQRLDNKIIRPSANYTGPEDLKFVPLAKRT"
     gene            5618..6061
                     /locus_tag="VLN25_03635"
     CDS             5618..6061
                     /locus_tag="VLN25_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSQ09695.1"
                     /translation="MAEVTLNGLTVRRAQRVDLPRLLELLADDELGKNREGVGSDDPA
                     YIRAFDAIDRDPNQSLLLAELDGRVVGMLQVTFIPGLSRRGAWRANIEALRVDSSVRR
                     RGIGGWLLSRALDNARKRGCRLAQLTSDRRRSEAHRFYERLGFTD"
     gene            6097..6453
                     /locus_tag="VLN25_03640"
     CDS             6097..6453
                     /locus_tag="VLN25_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09696.1"
                     /translation="MTARQLLSLAAAFIASAASAAEPRLVTVVYLCDEARVLSIVQPE
                     MSLPGQNLELVFGSERKRMQPAIATSGAHYLSTDGAWEWWSRGAVSRLATSSGSALAQ
                     NCTVFQAGGKRPAPDK"
     gene            6511..>6936
                     /locus_tag="VLN25_03645"
     CDS             6511..>6936
                     /locus_tag="VLN25_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HSQ09697.1"
                     /translation="MGSHTSNVRPKPDKVLVDIADYVIKYKVKSKEAIDTARYCLMDT
                     LGCGFEALEYPACTKMLGPVVHGTVVPNGAKVPGTQFQMDPVKAAFDIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVAAGKSPLTVS"
BASE COUNT         1377 a         2283 c         2165 g         1111 t
ORIGIN      
        1 ccgccgaacg gcctctggta gatcgtgccg tccggattgc ggtcaaacgg cattccgaaa
       61 tgctcgagtt cgtacacgac cttcgacgcc tcacggcaca tgaactcgat cgcatcctgg
      121 tcgccgaggt agtccgagcc cttgaccgtg tcgaacatgt gccacagcca gttgtcctcg
      181 gtcatattgc caagcgatgc gccgatgcct ccctgcgcag cgaccgtgtg cgagcgcgtc
      241 ggaaacacct tggacagcac cgcgacgttc agcccggctt gcgccagctg cagcgagcac
      301 cgcatgcccg agccacccgc gccaacgatc accgcgtcga agcgacgacg agctactttc
      361 gatgcagccg ccatgaatca gactctccag agaatctgca cggaccatac cgcgcagaac
      421 accagccaca gtaccgtcag cgtctgcaga gctacccgga tccctgtcgg cttgacatag
      481 tccatccaga tatcacgcac gccgatccat gcgtgataga aaagcgccag cagcgccacc
      541 agcgtgacga tcttcatcca gagcgggaca aaaagccctg cccagccgtc gtagccgagg
      601 gggccgccga aaaccagccg cagcagaagg aacaccacga ataccgtcag cacgatggcc
      661 gtcagccgct gcaacaagaa cccaccgacg ccgtagtgcg cgccaacgac gattcgcttg
      721 gtaccgatcc ctgcggggtt gggcgacgcg ggcatcagaa cgctccgaag atcttcaatg
      781 cggccagcag agtcagcggc aggctaattg cgaaaacggc gagcgcgctg gtacgcgcgg
      841 ccggtttgtc aacgccgaca tccaggtcga gcaagaggaa gcgcagaccg gcgatcaggt
      901 ggtgcaggaa tgaccacgcc agcccgacca gaaccagttt taccagccag tgcgcgccga
      961 agtcagcgag gcgcgcgtag ctcagctcgg acgtgaggct caggtcgaac agccacagga
     1021 tgaggggcag tgtcaggaac agcgccgccc cgctgatccg atgcaggatc gagaccatgc
     1081 ccggcggcgg aagcttgtat ttcaggagct gcgaaacgtg gatgttgcgg aattcagggc
     1141 ggggtttgct cgcgaaagca ggtgtggtca ttgacgatgg cctgtgtggt tcgtgccctg
     1201 gataggcgcg ctattttgaa cgatttgatg aggcgaacca aatcacgaca gcgtgttgtt
     1261 gtagtgatag tggtcggtga cgcacaaacc gcgccggact tcgaccggtc gatcaccgta
     1321 cgagtacgag acccgctcga ccagcagcag cggtgacttc tcggggacac cgagcagctg
     1381 tgccgcatcg cgctctgcgc cgatggcccg caactgttcc tcggcgcgaa tcatccgggt
     1441 tccgaactcg gtctcgaaaa ggccatacag cgggcccttg tattcggaca gtcgttcggc
     1501 cgtcagcccc ttgaacagac cccccggcaa ccagatctcg tcgagaacga tcgggtcgcc
     1561 gccaaagctc agcaggcgcc gcagatacac cgcggcatcg ccggtacgca ggtcgagcag
     1621 ccgcgcaata tcagccggcg cgcggacacg ccgacattcc aggaatcggc tctgcggtgg
     1681 ggcgggttcg ccgccgtcag gaaccacccg cagaaaccgg aactgcgcgc gcggctcgtg
     1741 atgtgtgcca acgaaggtgc ccttgtcctg ccggcgtacc agcaggttct cggccgcgag
     1801 ttcatcgacg gccttgcgga cggttccctg gctgacctgg aaccgggtgg cgagctcgat
     1861 ttcacttggg atgagttcgc cgggcctcca ttccccctca tccaggctgc gcaacaacag
     1921 gcccttgatc tggcggtaca gcggcgaaaa aaccgccggc cgcgccacac ggtcaggact
     1981 gtctgcgggg aagtcagcgc tgggcatgag ctgagttcag cataactgat agagaaatga
     2041 cgtggattaa ttcttatgtc ttatataaga tagtattatc gtcccaggtt gcagcattgg
     2101 cagcccccga tgcagcaccc gccataaaga agcctcggaa attctgctgc cgccacgatc
     2161 gcacatgagc cttcttcccc tgcagtctgt ccaggctagt tgtggccctt ctcacggaat
     2221 tcgttcgggg caccgtggtc gccggaagtc ggcgcgctat agcgggcaca cggtccggat
     2281 ccaacaccgc ccgggctccg caccggccga gcccggcccg gcacagcgcg ccacaccaag
     2341 ctacgtcaag gcgtgcgcgc gacagcggca gtacactcct gcggctcaca cgccggcgtt
     2401 tgcccaccgg ttcgcacgac gaattccacg ctcgcccggc tgccgccgac ctcgcccgcc
     2461 attctcatcg tccactgcat ctccaggaga ccatttccat gagcaaagct cccgtacgcg
     2521 ttgccgtcac cggcgctgcc ggacagatcg gttattcgct gctgttccgc atcgcctccg
     2581 gcgaaatgct gggcagggac cagccggttg tcctgcaact gctcgagatc ccggacgaaa
     2641 aggcgcaaaa ggccctcaag ggcgtgatga tggaactcga cgactgcgcg ttcccgctgc
     2701 tggccggcat ggaggcgcac agcgacccga ccgccgcatt caaagacacc gagatcgcgc
     2761 tgctggtcgg cgcacgcccg cgcggccccg gcatggaacg caaggacctg ctcgccgcca
     2821 acgcacagat tttcacggcg cagggtaagg cgctggacaa ggccgccagc cgcaatgtca
     2881 aggtgctggt cgtcggcaac ccggccaaca ccaacgccta catcgcgatg aagtccgcgc
     2941 cgtcgctgcc caagggcaat ttcacggcca tgatgcggct cgaccacaac cgcgcgctgt
     3001 cgcagctcgc cggcaagacc ggcaagccgg tcgcctcgat cgagaaactg gtggtgtggg
     3061 gcaaccactc tccgacgatg tatccggaca tccgctttgc aacgatcggc gggcagccgg
     3121 cccagccgat ggtcgacccg acctggtatc gcgacaccta catgccgacc gtcggcaagc
     3181 gtggcgcggc gatcatcgag gcgcgcggcc tgtcgtcggc cgcctccgcc gccaatgcgg
     3241 cgatcgacca catccgcgac tgggtgctcg gcacgaacgg ccgctgggtg acgatgggtg
     3301 tgccgtccga cggcagctat ggagtgcccg aagaaatcat ctacggtgtc ccgtgcgtct
     3361 gcgccggtgg caagtacgaa gtggtcaagg gcctggccat cgacgagttc tcgcgcggca
     3421 agatggacaa cacgctcaag gagctggtcg aggaacgcga cggcgtcaag cacctgcttg
     3481 gctgagcccg acgtggcgac cctcgcagcc tctaaggaga aatgccgatg agtccctccg
     3541 ccggagcccg ctttcgcgag gcgctgcaac tggaaaggcc cctgcagatc gtcggaacca
     3601 tcatcgccaa ccacgcgctg atggcgcggc atgtcgggta ccgcgcgatc tacctgtcgg
     3661 gcggcggcgt ggccgccggc tcgctcggca tgccggacct ggggatcaac acgctcgacg
     3721 acgtcctcac cgacacccgc cgcatcactg acgtgtgcga cctgccgctg ctggtcgaca
     3781 tcgacaccgg cttcggcccc agcgccttca acatcgcccg cacggtcaag tcgttgatca
     3841 aggccggcgc tgccgcctgt catatcgaag accaggtggg tgccaagcgc tgcggccatc
     3901 ggcccggcaa ggaaatcgtc acgaccgaag agatggtcga ccgcgtcagg gcggctgccg
     3961 atgcgaagac cgactccgcc ttcttcctga tcgcgcgcac cgatgcgatc gcggtcgagg
     4021 gaatcgatcg tgcgatcgag cgcgccgtgg cctgcgcgga ggccggcgcc gacggcattt
     4081 tcgcggaggc cgccaaagat ctcgccacct accgacgctt cgtggacgcg gtgaaggtgc
     4141 cggtgctcgc gaacatcacc gagttcggcg cgacgccgct tttcacggtc aaggagctcg
     4201 gcgacgccgg ggcggcgatg gtgctgtatc cgctgtcggc gtttcgcgcc atgaacaagg
     4261 cggcccttaa cgtctacacg acgctgcgcc gagacggcac gcagcagggg gtcgtcgaaa
     4321 ccatgcagac acgaatggaa ctgtacgacg tgatcggcta ccaggcgtac gaagatcgcc
     4381 ttgaccagct gtttgcaaag cgcaagtagg ccgtgcctcg acgcagggcc gacaccgaac
     4441 gagagagctg aggaggacag atgaacgaca cacccgcacc cgaagcgccc aagcccaaga
     4501 aatccgtggc cttgtccggc gtggtggccg gaaatacagc gttgtgtacc gttgggcgca
     4561 ccggcaacga ccttcactac cgcggttacg acatcctcga catcgctgat acctgcgagt
     4621 tcgaggaaat cgcgcacctg ctggtgcatg gcaagctgcc gaaccacagt gagctggtgg
     4681 cctacaagac gaaactcaag gcgctgcgcg gactgccggc caatgtcaag gctgcgctcg
     4741 agtggctgcc ggcgtcgagc catccgatgg acgtgatgcg cgcgggagtg tctgcgctcg
     4801 gctgcgtgct gccagagaag gacgaccaca acgtccccgg cgccaaggac atcgccgatc
     4861 gcctcatggc ttcgctcgga tcgatgctgc tctactggta ccactacagc atcaacggcc
     4921 gacgcatcga ggtcgagacc gacgacgaca cgattggcgg gcacttcctg cacttgctgc
     4981 acgggaagcc cccgagccag ctttgggtca aggcgatgca cacttcgctg attctgtacg
     5041 cagagcacga gttcaatgct tcgacgttca ctgcgcgcgt gatcgcgggg acgggctcgg
     5101 acttctactc ggcgatcgcc ggtgcgatcg gcgccctgcg cgggccgaag cacggcggcg
     5161 ccaacgaggt cgcgttcgag atccagaagc gctacgagac gccggacgag gccgaggccg
     5221 acatccgccg ccgcgtcgag gccaaggaag tggtcatggg attcgggcat ccggtgtaca
     5281 ccgtctccga cccgcgcaac aaggtgatca aggaggtcgc acgcgccctg tcgaaggata
     5341 tcggcaacac gaagatgttc ggcatcgccg agcggctcga aaccgtgatg tgggacgcca
     5401 agaagatgtt cccgaatctc gactggttca gcgccgtcag ctatcacatg ctgggcgttc
     5461 ccacgagcat gtttacgccg cttttcgtga tctcgcgaac cagcggctgg agcgcgcaca
     5521 tcatcgagca gcgcctcgac aacaagatca tccgtccgag tgccaactac accggcccgg
     5581 aagacctgaa gttcgtcccg ctggcgaaac ggacctgatg gctgaagtca cgctcaacgg
     5641 cctgacggtg cgtcgcgcgc aacgagtcga cctgccacgc ctgctcgaac tgctggcgga
     5701 cgacgaactc ggcaagaacc gggagggcgt cggcagtgac gatcctgcct acatccgggc
     5761 attcgacgca atcgaccgcg acccgaacca gagcctgctg ctcgccgaac tggatggccg
     5821 ggtcgtcggc atgctgcagg tcactttcat tcccggcctg agccgacgcg gcgcctggcg
     5881 cgccaacatc gaagcgttgc gcgtcgacag cagcgtacgc cggcgaggaa tcggcggttg
     5941 gctcctgtcg cgcgcgctcg acaacgcacg taagcgcggc tgccggctgg cccagctgac
     6001 cagcgaccgg cggcggtcag aggcccatcg cttctacgag cggctcggat ttacggacta
     6061 gcacatcgga tacaagcttc ttctggagtc ttccgagtga ctgcgaggca gcttctgtcc
     6121 ctcgctgctg ccttcatcgc aagtgccgcc tcggccgccg aaccgcgcct ggtgaccgtc
     6181 gtgtacctct gcgatgaggc acgcgtgctg agcatcgtgc agccggaaat gtcgttgccc
     6241 ggccagaacc tggaactcgt cttcggatcg gagcgcaagc gcatgcagcc cgcgattgcg
     6301 acctcgggtg cgcactacct gtccaccgac ggcgcctggg aatggtggag caggggtgcc
     6361 gtcagccggc tcgcgacctc cagcggttcc gcgctggcac agaactgcac cgtcttccag
     6421 gccggcggca agcgacccgc gccggacaaa tagacccatc gaacatcgac acgaaccatc
     6481 gaacactgga gcagaaagga tcatcccctc atgggaagcc acaccagcaa cgtccgcccg
     6541 aagcctgaca aggtgctggt cgacatcgcc gactacgtca tcaaatacaa ggtcaagagc
     6601 aaggaagcaa ttgacaccgc gcgctactgt ctgatggaca cgctcggctg cggcttcgag
     6661 gcgctcgagt atccggcctg caccaaaatg ctcggtccgg tggtccatgg aaccgtggtg
     6721 ccgaacggcg caaaggtgcc gggtacgcag ttccagatgg atccggtgaa agcggcgttc
     6781 gacatcggcg cgatgatccg ctggcttgac ttcaacgaca cgtggctcgc ggccgaatgg
     6841 ggccatccct cggacaacct gggcggcatc ctggcaaccg ccgactggct ttcccgcaac
     6901 gcggtcgccg ctggaaagtc accgctaacg gtatcc
//
LOCUS       DATIRT010000153         8981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_708099, whole genome shotgun sequence.
ACCESSION   DATIRT010000153 DATIRT010000000
VERSION     DATIRT010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8981
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708099"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VLN25_03650"
     CDS             complement(<1..216)
                     /locus_tag="VLN25_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09698.1"
                     /translation="MSNPMWEWWKLALSASEIAWYSPQVVQKRTQRLLAAPTLTRAAD
                     RKEATRMVAEKLQAGQEAQWALWQQSLA"
     gene            complement(213..812)
                     /locus_tag="VLN25_03655"
     CDS             complement(213..812)
                     /locus_tag="VLN25_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HSQ09699.1"
                     /translation="MAAEPVGLLLAAGLGTRYDPTGRSLKLLAPARRGPHVGAPMAAA
                     AARALLAAMPRVTAVVRPADSDAQRQLHALLRSEGCTLAVCHNADAGMSASIARGVQA
                     EERAAGWVIGLADMPAIEVSTVRAVVGLLLEGALTAAPMFRGQRGHPVGFAAELRPRL
                     LALSGDAGARPVLAAHPPRVIEVDDPGVLYDVDTVEEKR"
     gene            complement(802..2193)
                     /locus_tag="VLN25_03660"
     CDS             complement(802..2193)
                     /locus_tag="VLN25_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HSQ09700.1"
                     /translation="MSEPSLSPSSAPRAALAPIAAPKPALSVFDAVMITVGIVIGAGI
                     FKTPSMVAGVTGSVDWMLIAWVLGGVLSLVGALCYAELASAFPHAGGDYHFITKAFGR
                     DASFFFAWARVMVITTGSIALLAFVFGDYMSRVVSLGAQSSAIYAALTVVVLTVINLI
                     GLRESARTQNLLTILEVSGLLLVALARLIATAPAESSSATPTASGMPAMFGLAMVFVL
                     LTYGGWNEAAYISAEVKGGPRAIVRTLVLSLLVITVVYVLVVLAVLKGLGFSGLAKST
                     AVGADVMQAAFGPVGAQLIGAIVGVATLTSINATMIVGARTNYALGRDWPVLAFMSHW
                     HGERHVPVIAFLVQAAIALALIAFGSVQKDGFSTMVEFTAPVFWFFFLLTGVALFVLR
                     FRAPHVARPFKVPMYPILPIVFVGTCAYLLYSSVTYAQSQNAVHVALYVMGAGVVAWI
                     VMRLVATRRPDGG"
     gene            complement(2266..3183)
                     /locus_tag="VLN25_03665"
     CDS             complement(2266..3183)
                     /locus_tag="VLN25_03665"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HSQ09701.1"
                     /translation="MSRTVVRPLAIGAACLLTVALIGDASARTRAECERDYKPQVGQA
                     GKDVVWVPTPDELLQRMLRMAKVTPQDLVFDLGAGDGKIAIAAGKLGANSVGIEYNPD
                     MAKLASCLVTAEGVGNKTRIIQGDIFKEDFSKATVITMYLLPELNLCVRHRILGMKPG
                     TRVTSHQFTMGDWEADETSEFEYRTAYLWIVPARVDGTWVLRDASGASTAVNLTQTYQ
                     KLTGDVVSGAPKQPLVGATLRGDQLRFAFNDDKGVTRSFTGSVRGNELVGTLKSGSVE
                     TKVTGAPQGALRAAAWAEMQPACGRFYGK"
     gene            complement(3285..5507)
                     /locus_tag="VLN25_03670"
     CDS             complement(3285..5507)
                     /locus_tag="VLN25_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HSQ09702.1"
                     /translation="MRFIEQIAQAQRDAAATMVPVKLSRREFMKATGVAGGGLMLGLG
                     AVPADAQEAKRMVYPPSAFVRIAPDGRTTVMVNRLEFGQGVNTALPMLLAEELDCEWD
                     KVRAELAPAAEVYADPMFGIQMTGGSMSVSSSWKQYRVIGASARAMLIAAAAQQWGVE
                     PAKLDTRSGFVIELAGQKRRVSYGSLAEAAAKQPVPTRIGLKADPGDFFFIGRPTRRL
                     DSREKVNGTAQFGIDKSLPNLRVAVLVHPPVFGGKVASFDPAKARAIKGVEAVVQVPV
                     DRGGTGVAVIASGYWPAKTGRDALDIKWEAPTGPSTDDLVRQFRELAGKPGLLARSDG
                     DANAVAQAATRIEATYEFPYLAHAPMEPLNAVVDLKADHCTVWCGTQFQTIDQLAIAS
                     TAGLKPEQVTLNTMLAGGGFGRRAIPSSDYLVEAVNIAKAMKQSGIDAPVKVIWSRED
                     DIRGGYYRPLVVHRVVAGLDAGNTLRGWNHSIVGQSLLKGTPFEKYLVKDGIDSTTTE
                     GIVDTPYRLPNLQVSVHHPEVSVPVLWWRSVGHSHTAFVMETMVDELARAAKQDPIAY
                     RLALLDPKHTRVRGVLQFVREKSGWTTPVAKGRARGVAVHESFGSVVAHVVEVSVDKN
                     VIRVHKVTSAIDCGTAVNPLTIEAQIQSGMVFGLSAALYGRITLKDGVVQQSNFTDYP
                     VLRINEMPEISVHIVPSRADPTGVGEPGTPPIAPAVANAVFALTGKRLRKLPFDLSQA
                     "
     gene            complement(5517..5972)
                     /locus_tag="VLN25_03675"
     CDS             complement(5517..5972)
                     /locus_tag="VLN25_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HSQ09703.1"
                     /translation="MTRLNVNGKAVDVDADPATPLLWALRDNLGLTGTKFGCGIAACG
                     ACTVHVNGQPVRSCITPISSVSGPVTTIEAAASDRVGKAVQDAWVKLDVVQCGYCQSG
                     QVMAATALLKGKKNPSDADIDAALAGILCRCGTYARIRAAVKDAARSLA"
     gene            complement(6032..7027)
                     /locus_tag="VLN25_03680"
     CDS             complement(6032..7027)
                     /locus_tag="VLN25_03680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HSQ09704.1"
                     /translation="MTQAIVLSKEGDAFRAEIRAVTDAELEAATPGDDVVIGVEYSTI
                     NYKDGLALTNTSPVVRKWPMVAGIDAAGKVIHSTHARWKAGDAVVINGWGLGESHWGG
                     LSQRARVKGDWLVRIPAPFTARDAMAIGTAGYTAALSVLALQAHGVTPTHGEILVTGA
                     TGGVGSVAVALLSSLGYTVVGSTGKAAEGDYLKSLGAHEVIDRASLAVAGKPLQKDRW
                     AGAIDSVGSVTLANVCAQTRSGGTVAACGLAGGMDFPASVAPFILRGVTLAGIDSVMA
                     PIERRKAAWKLLAEQLPVETLAQLTQEVPLAGAFDVARDILAGRVRGRVVVDVNR"
     gene            complement(7091..7567)
                     /locus_tag="VLN25_03685"
     CDS             complement(7091..7567)
                     /locus_tag="VLN25_03685"
                     /inference="COORDINATES: protein motif:HMM:NF023338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3429 domain-containing protein"
                     /protein_id="HSQ09705.1"
                     /translation="MSVVIEQRIATLLGNLGLLPFFVLALLCWPWGGTAAFRMQLGLA
                     GYAAVVLSFLGAVHWGLALANPGLNKAQSWNALGWGVMPSLLGWLALLMILFGVPATI
                     VFAFLIGDLLLVRVVDGLLMKLYSTPVASWYLALRTRLTIAASLCLLIAAIGAFPR"
     gene            7777..>8981
                     /gene="hemG"
                     /locus_tag="VLN25_03690"
     CDS             7777..>8981
                     /gene="hemG"
                     /locus_tag="VLN25_03690"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HSQ09706.1"
                     /translation="MAFDVIVVGAGLSGLVTAYRLQRAGLSVQVLEAAGRPGGVIGSE
                     RRSGALFERGPNSGMDTSPVINALLDDLGIRSQRLDASKASSRRYVLRGGRLVALPTS
                     GGSFIATPLFSLGAKLRLFAEPFIARAPSEAEESIAGFVRRRLGREFLDYAVEPFVSG
                     IYAGDPEQISVSAAFPRLHALEQRYGSLLRGAILGARERRRKAEKSKSAAVSFSFRDG
                     MQTLTDALAAAITDLQCGARVVQLAREADGSYVVTVDRAGHADQSATLRARAIVLAVP
                     AHAGAALVRPLAPDAAVSLEAIPYAPVVVVASAYRRSDVGHPLDGFGFLAPAREQPRV
                     LGSLFSSTMFEHRAPSDTVVFTTFVGGRRNPARAVASDEELLQMVQYELERLVAVCGT
                     PLFNEITRWP"
BASE COUNT         1556 a         3008 c         2942 g         1475 t
ORIGIN      
        1 ggccagggac tgctgccaga gggcccactg ggcttcctgg ccggcctgca gcttctccgc
       61 caccatgcgc gtggcttcct tccggtcggc cgctcgcgtc agcgtcggtg cggccaggag
      121 ccgctgggtg cgcttctgca cgacctgcgg cgaataccag gcgatctcgc tcgcgctcaa
      181 cgccagcttc caccactccc acatcggatt gctcatcgtt tctcctcgac agtatcgacg
      241 tcatacagca cgcccggatc atccacctcg atcactcgcg gcggatgcgc cgccagtacc
      301 ggccgggcac ccgcgtcgcc gctcaatgcc agcagtcgag ggcgcagttc agccgcgaag
      361 ccgaccgggt gcccgcgctg gccgcgaaac atgggtgccg ccgtcagtgc gccttcaagc
      421 agaagtccga cgacggcgcg cacggttgag acttcgatcg ccggcatgtc cgcgaggccg
      481 atgacccatc ccgcagcgcg ttcttctgcc tggaccccgc gcgcgatcga tgcgctcatg
      541 ccggcatcgg cgttgtggca caccgcgagc gtgcagcctt cgctgcgcag cagggcatgc
      601 agctggcgct gggcgtcgct gtcggccgga cgcacgacgg cagtgacccg gggcatcgcc
      661 gcaagcagtg cccgcgcggc agcggcagcc atcggggcgc cgacatgcgg accgcgccgc
      721 gcgggagcca gcagtttcag cgagcggccg gtcgggtcat agcgtgtccc aagcccggcg
      781 gcaagcagga gtccgaccgg ttcagccgcc atccggacgg cgcgtcgcga cgagccgcat
      841 cacaatccat gcgacgacgc cggcgcccat cacatacagc gccacatgca cagcgttctg
      901 tgactgcgca tacgtcacgc tcgagtacag caggtaggcg caggtgccga cgaagacgat
      961 cggcaggatc ggatacatgg gtaccttgaa tggccgcgcc acgtgcggtg cgcgaaaccg
     1021 cagcacgaac aacgccacgc ccgtcagcag gaagaagaac cagaacactg gcgccgtgaa
     1081 ctcgaccatc gtcgagaagc cgtccttttg taccgacccg aacgcgatga gtgcgagcgc
     1141 gatcgcggcc tgaacgagaa acgcgatcac gggcacgtgc ctttcgccat gccagtggct
     1201 catgaatgcc agcaccggcc agtcgcgacc aagcgcgtag ttcgtgcggg caccgacgat
     1261 catcgtggcg ttgatcgacg tcagcgtcgc aacgccgaca atcgcgccga tcagttgggc
     1321 acccacgggt ccgaatgccg cctgcatgac gtcggcgccg accgcggtgc tcttggccag
     1381 accgctgaat ccgaggccct tgaggactgc cagtacgacg agcacgtaga cgaccgtgat
     1441 gacgagcagc gagagcacca gcgttcgcac gatcgcgcgt ggcccaccct tgacctcggc
     1501 ggaaatgtac gccgcctcgt tccagccgcc gtacgtgagc aacacgaaga ccatcgccag
     1561 cccgaacatg gcgggcattc cagaggccgt cggggtggcg gacgaagact cggcgggcgc
     1621 tgtcgcgatc agccgggcga gcgccaccag cagcaggcca ctgacctcta ggatcgtcag
     1681 caggttctgg gtgcgtgccg attcgcgcag gccgatgagg ttgatgacag tcagcacgac
     1741 gaccgtgagc gcggcataaa tcgcagacga ctgtgctcca agcgacacca cccggctcat
     1801 gtagtcgccg aagacaaatg ccagcagcgc gatcgagccg gtggtgatca ccatcacgcg
     1861 ggcccaggca aagaagaagc tggcatcgcg gccaaaggcc ttggtgatga agtgatagtc
     1921 accgccggcg tgcgggaagg cgctcgcaag ctccgcatag caaagcgcgc cgaccagcga
     1981 cagcacgccg cccagaaccc aggcaatcag catccagtca accgagccgg taacgccggc
     2041 caccatcgag ggtgtcttga aaatcccggc gccgatcacg atgccgacgg tgatcatgac
     2101 cgcgtcgaac actgaaagcg ccggcttcgg cgcagcgatc ggagcgagcg cggcgcgcgg
     2161 tgcggacgac ggagacagtg acggttcgct catcgagcgt ccttccatgt aaaaaagagc
     2221 cccgccgtag cggggccctg ctgcgttcag gaaaccggcg cctgtctact taccgtagaa
     2281 gcgtccgcac gccggctgca tttcggccca ggcggcggcg cgcaaggcgc cttgcggcgc
     2341 gccggtgacc ttggtctcga cgctgccgct cttgagcgtg ccaaccagtt cgttgcctcg
     2401 aaccgagccg gtgaagctgc gcgtcacgcc cttgtcgtcg ttgaaggcaa agcgcagctg
     2461 atcgccgcgc agcgtcgctc ccacgagcgg ctgcttgggc gcgccgctca cgacgtcacc
     2521 ggtaagtttc tggtatgtct gcgtgagatt caccgctgtg ctggcgccgc tcgcgtcgcg
     2581 caggacccag gttccgtcca cgcgcgccgg gacgatccac aggtacgcgg tgcggtactc
     2641 gaactccgag gtctcgtccg cttcccagtc gcccatcgtg aactggtgcg aggtcacgcg
     2701 cgtgccgggc ttcatgccga ggatgcgatg acgcacgcac agattcagtt ccggcaacag
     2761 gtacatcgtg atgacggtgg ccttgctgaa gtcttccttg aaaatgtcgc cctggatgat
     2821 tcgggtcttg ttgccgacac cttcggcggt cacgaggcag ctggccagct tggccatgtc
     2881 cgggttgtat tcgatgccga ccgagttggc gccaagcttg cccgcggcga tcgcgatctt
     2941 gccgtcgccg gcgccgaggt caaaaaccag gtcctgcggc gtgaccttgg ccatgcgcag
     3001 catgcgctgc aagagttcat cgggcgtcgg cacccacacg acatccttgc cggcctggcc
     3061 gacctggggt ttgtagtcac gctcgcactc cgcgcgagtg cgcgccgacg cgtcgccgat
     3121 caatgcgacg gtgagaaggc acgcggctcc aatggcaagc ggacgtacaa cagtgcgaga
     3181 catcatgtgg gctcctgcgg aaggggtcgt gggggatcgg cattctgccg aaagtctctg
     3241 cggccgtcag ccgcagggtg caagtgaaat cgactgggca atcgtcaggc ctggctgaga
     3301 tcgaacggca gcttgcgcag ccgcttgccg gtcagggcaa agaccgcgtt ggcgaccgcc
     3361 ggtgcaatcg gcggcgtgcc cggctcaccg acaccggtcg gatcggcgcg actcgggaca
     3421 atgtgcacgc tgatctcggg catctcgttg atgcgcagca ccgggtagtc ggtgaagttg
     3481 gattgctgca cgacgccgtc cttcaacgtg atccggccat acagggccgc cgataggccg
     3541 aacaccatcc ccgactggat ctgcgcttcg atcgtcagcg ggttgactgc cgtgccgcag
     3601 tcgatcgccg aggtgacctt gtggacccgg atgacgttct tgtcgaccga gacctcgacc
     3661 acgtgcgcca cgaccgaccc gaatgactcg tgcacggcga cacctcgggc gcgtcccttg
     3721 gccaccgggg tggtccagcc ggacttctca cgcacgaact gaagcacgcc ccgtacgcgc
     3781 gtgtgcttcg ggtcgagcag cgccagacga tacgcgatcg gatcctgctt cgcggcgcgg
     3841 gccagctcgt cgaccatcgt ctccatgaca aacgccgtgt gcgaatggcc aaccgagcgc
     3901 caccacagca ccggcacgct gacctcgggg tgatgcaccg agacctgcag gttcggcagg
     3961 cggtacgggg tatcgacgat gccctcggtg gtcgtggagt cgatgccgtc cttcacgagg
     4021 tacttctcga aaggcgtgcc cttcaatagt gactgcccga cgatgctgtg gttccagccg
     4081 cgcagcgtgt ttccggcgtc gagacccgcc accacgcggt gcacgaccag cggccggtag
     4141 taaccgccgc ggatgtcatc ttcgcgcgac cagatcacct tgactggggc atcgatgccg
     4201 gattgcttca tcgccttcgc gatgttcacc gcctccacga ggtaatcgga cgacgggatg
     4261 gcgcgccggc cgaatccacc gccagccagc atcgtgttca acgtgacctg ctccggcttg
     4321 agcccggcgg tgcttgcgat ggcgagttga tcaatggtct ggaactgtgt gccgcaccac
     4381 acggtgcagt gatcggcctt gaggtcgacg accgcattca gtggctccat cggcgcatgg
     4441 gccaggtacg gaaattcgta ggtggcctcg atcctcgtcg cagcctgggc cacggcattg
     4501 gcatcgccgt cgctgcgcgc cagcaatccc ggcttgccag ccagttcgcg gaattgcctg
     4561 acaaggtcgt ctgtggacgg gcccgttggg gcctcccact tgatgtccag cgcatcacga
     4621 cccgtcttgg ccggccagta gccgctggca atcacggcca ctccggtgcc acctcgatcg
     4681 accggtacct ggacgacagc ctccacgccc ttgatggcac gtgccttggc cggatcgaag
     4741 ctcgccacct tgccgccgaa caccggcgga tgcaccagga cggcgacccg caggttggga
     4801 agcgacttgt cgatgccgaa ctgggcggtg ccgttgacct tctctcgcga gtccagtcgc
     4861 cttgtcggtc gaccgatgaa gaagaagtcg ccgggatcgg ccttgagccc gatgcgcgtc
     4921 ggcaccggct gcttggcggc cgcttcggcc agcgagccgt agctgacgcg tcgtttctgc
     4981 ccggccaact cgatgacgaa gccgctgcgg gtgtcgagct tcgcgggttc cacaccccac
     5041 tgctgggcgg cagcagcgat cagcatcgcg cgcgccgatg cgccaatcac gcggtactgt
     5101 ttccagctcg acgaaaccga catcgagccg ccggtcatct ggatgccgaa catcggatcc
     5161 gcgtagacct ctgccgccgg tgccagttcg gcgcgaacct tgtcccattc gcagtcgagc
     5221 tcttccgcga gcagcatcgg cagcgctgtg ttgacgccct ggccgaactc cagccggttg
     5281 accatcaccg tggtgcggcc gtctggcgca atgcgcacga aagccgatgg cggatagacc
     5341 atgcgcttgg cttcctgggc gtctgcgggg acggcaccga ggccgagcat cagtccgccg
     5401 ccggccacac cggtggcctt catgaactcg cgccgcgaga gcttgaccgg caccatcgtc
     5461 gcggcagcgt cacgctgcgc ctgggcaatc tgttcgatga atcgcatcgt cgtctcctag
     5521 gcgagcgaac gcgccgcgtc cttgactgct gcgcggatgc gcgcataggt cccgcagcga
     5581 cagaggatgc cggcgagcgc tgcgtcgatg tcggcatcac tcggattctt cttgcccttg
     5641 agcagcgccg tggccgccat cacctggccc gactggcagt agccgcactg cacgacatcg
     5701 agcttgaccc aggcgtcctg caccgccttg ccgacgcggt cactcgcggc ggcctcaatc
     5761 gtcgtcaccg ggccgctgac actcgagatt ggcgtgatgc atgaacgcac cggttgaccg
     5821 ttcacatgca ccgtgcaggc accgcaggcg gcgatgccac agccgaactt cgtcccggtc
     5881 agtccgaggt tgtcgcgcag cgcccagagc aagggcgttg cgggatcggc gtcgacgtcg
     5941 acggccttgc cgttcacgtt caaacgggtc atgacgactc caggtaatga aatggggaga
     6001 acggtggttg atcgtgccgc tgcaggcggc ttcagcgatt gacgtcgacg accacccggc
     6061 cgcggacgcg cccggcgagg atatcgcggg ccacatcgaa tgctcccgcc agcggcactt
     6121 cctgcgtcaa ctgtgccaga gtttcgaccg gtaattgctc tgccagcagt ttccatgcgg
     6181 ccttacgccg ctcgatcgga gccatcacgc tgtcgatgcc tgccagcgtc acccctctga
     6241 ggataaatgg cgccactgag gcgggaaagt ccatgccccc ggcgagaccg caggcagcga
     6301 ccgtaccgcc cgagcgtgtt tgcgcgcaga cattggcgag cgtcacgcta ccgacggaat
     6361 cgatcgcgcc cgcccaacgg tccttctgga gcggtttgcc cgcgaccgca agcgaggcgc
     6421 ggtcaatgac ttcgtgggct cccagggact tcaggtagtc gccttcggcc gccttgccgg
     6481 tcgagccgac cacggtgtag ccgagcgatg acagcagcgc caccgcgacc gaaccgacgc
     6541 ctcccgtcgc accggtgacg aggatctcgc catgagtcgg cgtgactccg tgggcctgca
     6601 gcgccagcac cgacagggcg gccgtgtagc ccgccgtgcc gatggccatc gcatcgcgcg
     6661 cagtgaaggg cgccggaatg cgcaccagcc agtcgccctt gacgcgcgcg cgctgggaca
     6721 ggccgcccca atgcgattcc ccgagtcccc agccattgat gacgacggca tcacccgcct
     6781 tccagcgggc gtgggtgctg tgaatcacct tgccggcggc atcgatgccg gcaaccatcg
     6841 gccacttgcg caccacgggc gacgtattcg tcagtgccag cccgtccttg tagttgatgg
     6901 tcgagtactc gacgccgatc acgacatcgt cccccggtgt cgcggcttcg agttcggcgt
     6961 ccgtgacggc acgaatctcg gcgcggaagg cgtcaccctc cttggaaagt acgatggctt
     7021 gcgtcatgtg ttgaaaaccc gggatgcagg accggccatc atagtgccgc gacgaaccac
     7081 gacgctcccg tcagcgcggg aaggcgccga tcgccgcgat gaggaggcac agcgaagcgg
     7141 cgatcgtcag ccgcgtccgc agcgccaggt accacgatgc caccggcgtc gaatacagtt
     7201 tcatcagcag gccatcgacc acccgcacca gcagcaggtc accgatcagg aacgcgaaca
     7261 caatggtcgc gggcacgcca aacaggatca tcagcagtgc gagccaaccc agcaacgacg
     7321 gcatcacgcc ccatccgagg gcattccagc tctgtgcctt gttcagtccg gggttcgcca
     7381 gcgcgagacc ccagtggacc gcgccgagga aggacagaac caccgccgca taaccggcga
     7441 gaccgagctg catccggaag gccgcggttc cgccccacgg ccagcacagc agcgccagca
     7501 cgaaaaacgg cagcaggccg aggttgccga gtagcgtggc gatgcgctgt tcgatgacga
     7561 ccgacatccg acctccgggt tcgccgcgat tatccagcct gcacaccaac aaccgtagct
     7621 tgcgttcgag caagtcgaga gcaggcggga ccccattggc caacgcatca ttggccgcgc
     7681 gcaaacgcgg atcagcgggc cggtggaatg ctctcgcgta cagggggagc aggctgacat
     7741 ggaaggagcg ggctgacatg gaaggagcag gctgacatgg cattcgatgt cattgtggtg
     7801 ggagccgggc tgtcggggct tgtgacggcc taccgacttc agcgcgccgg gctgtctgtc
     7861 caggtgctgg aagcggccgg gcggccgggc ggggtcatcg gctcggaacg ccgcagcggc
     7921 gcgctgttcg agcgcggacc caacagcggc atggacacgt ccccggtcat caatgcactg
     7981 ctggacgatc tcggcatccg cagccagcgc ctcgatgcca gcaaggcatc gtcccgtcgc
     8041 tatgtcctgc gtggcgggcg actggtggcc ttgccgacgt caggcggatc cttcattgcg
     8101 acgccgctct tttcgctggg ggccaagctg cggctcttcg cggaaccgtt catcgcgcgc
     8161 gcgccgtccg aagccgagga gtcgatcgcc ggattcgtgc ggcggcggct gggacgggag
     8221 tttctcgact atgccgtcga gccgtttgtc tcgggcattt atgccgggga tcccgagcag
     8281 atctcggtgt cggccgcgtt tccccggctg catgcgctcg agcagcgcta cgggagcctg
     8341 ctgcgcggag cgattctcgg tgcgcgcgag cgcaggcgga aagccgagaa gtcgaagagt
     8401 gccgcggtga gtttttcatt ccgcgacggc atgcagacgc tgaccgacgc gctggccgca
     8461 gcgatcacgg atttgcagtg cggcgctcgc gtggttcagc ttgcccgcga ggctgacggg
     8521 tcgtacgtcg tcacggtcga tcgggctggt catgctgacc agtcagccac tctgcgtgca
     8581 cgcgcgatcg tgcttgcggt gcccgcccat gcaggtgctg cgctcgtccg gccgctcgcg
     8641 cccgacgcgg cggtgtcact ggaggcaatc ccgtacgcgc cggtggtggt cgtggccagc
     8701 gcttatcgcc ggtccgacgt cggtcatccg ctcgacggct tcggtttcct cgcgccggca
     8761 cgggagcagc cgcgcgtgct cggatcgctc ttctcgagca cgatgttcga acatcgtgcg
     8821 ccgagcgata cagtggtgtt caccaccttt gtcggagggc ggcgcaatcc ggcgcgggcc
     8881 gttgcgtcgg acgaagaact gctgcagatg gttcagtacg agctggagcg attggtcgcc
     8941 gtgtgcggga caccgctctt caacgagatc acgcgctggc c
//
LOCUS       DATIRT010000154         2069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_708668, whole genome shotgun sequence.
ACCESSION   DATIRT010000154 DATIRT010000000
VERSION     DATIRT010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708668"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1143)
                     /locus_tag="VLN25_03695"
     CDS             complement(<1..1143)
                     /locus_tag="VLN25_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HSQ09707.1"
                     /translation="MSDKVVVAGVGMIPFRKPGDHPPYYLMGADAAKLALTDAAVAYA
                     QVQQAYVGYVYGDSTSGQRALYEVGLTGVPIINVNNNCSTGSTALFLARQAIESGAAD
                     CVLALGFEQMRPGALGAVFDDRPTPFDRFNEVTDTLVGEPQIPLALRYFGGAGREHMQ
                     RYGTPLETFAQIRAKASLHAQNNPLALFRKPVTIEQVLGDVQIWPGVMTRLMACPPTC
                     GAAAAALVSERYARQHGLDTGVRILAQSMTTDTASTFGRSMISLVGADMAKAAADAVY
                     EQAGIGPDDVVVCELHDCFAHNELISYEALGFCAPGEATRFVADGDNTYGGAVVTNPS
                     GGLLSKGHPLGATGLAQCFELTQQLRGRAEARQVDNARFGLQHNLGL"
     gene            complement(1158..>2069)
                     /locus_tag="VLN25_03700"
     CDS             complement(1158..>2069)
                     /locus_tag="VLN25_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSQ09708.1"
                     /translation="ELAAAGDTSFGVHVHIIVAHYLLNQGTEEQKRKYLPQLASGAMV
                     GAIAMSEPAAGSDLQGVRTRAERGDSGYVINGSKTFISNGYLADLIVVVARTDASAGA
                     RGLSLLLVETRNVEGFRVGRILEKVGQKGQDTCELFFDNVRVPGDAVLGGVEGRGFAQ
                     LMAELPYERTLVGIGAVAAIERALRLTVDYARERSAFGQKLIEMQNTRFKLAEIKTLA
                     VVARTFIDRCIMQVMDGTLDTATASMAKWYLTDLQCKVVDECLQLFGGYGYMLEYPIA
                     RMYVDARVQPIYGGTNEIMKEIIARSL"
BASE COUNT          336 a          702 c          677 g          354 t
ORIGIN      
        1 caggccgagg ttgtgctgca gcccgaagcg cgcgttgtcg acctgccgtg cctccgccct
       61 tccgcggagc tgctgcgtca gctcgaagca ctgggcgagc ccggttgcac cgagcggatg
      121 ccccttggac aacaggccgc cggacgggtt ggtgacgact gcgccaccat aggtgttgtc
      181 accatcggcg acgaaccgag tggcctcgcc cggtgcgcag aagcccagcg cctcgtagga
      241 gatcagctcg ttgtgcgcga agcagtcgtg cagttcgcac acaacgacgt cgtcgggccc
      301 gatgccggcc tgctcgtaga cggcgtccgc agcggccttc gccatgtcgg cgcctaccag
      361 gctaatcatc gaacgcccga atgtgctcgc ggtatccgtc gtcatcgact gcgcgagaat
      421 gcggacgccc gtatcgagcc cgtgctggcg cgcgtaacgc tcggagacga gggccgccgc
      481 ggccgcacca caggtcggtg gacacgccat cagccgcgtc atcacccccg gccagatctg
      541 cacgtcgccc agcacctgct cgatcgtgac cggtttgcgg aagagcgcga gcggattgtt
      601 ctgcgcgtgc aggcttgcct tggcccggat ctgcgcaaag gtctcgagcg gcgtgccgta
      661 gcgctgcatg tgctcgcgcc cggcgccgcc gaagtagcgc agcgcgagcg ggatctgagg
      721 ctcgccaacc agggtgtcgg ttacttcgtt gaagcgatcg aacggggtcg ggcgatcgtc
      781 gaacacggcg ccgagagcgc cgggacgcat ctgctcgaag cccagagcca gcacgcagtc
      841 ggcggctccg ctctcgatcg cctgccgcgc gaggaagagc gcggtcgaac cggtcgagca
      901 gttgttgttg acgttgatga tcggcacgcc ggtcagtccg acttcgtaca gcgcccgctg
      961 accgctggtg gaatcgccgt agacgtagcc gacataggcc tgctgcacct gcgcatacgc
     1021 gactgcggcg tcggtgagcg cgagtttcgc ggcgtccgcg cccatcaagt agtacggcgg
     1081 atggtcgccg ggcttgcgga aggggatcat gccgaccccg gcgaccacga ccttgtcaga
     1141 catttcagcc tccggcgcta cagcgaacgc gcgatgatct ctttcatgat ttcgttggtg
     1201 ccgccgtaga tcggctgcac gcgcgcgtcg acatacatgc gcgcgatcgg gtactcgagc
     1261 atgtacccgt agccaccgaa gagctgcagg cactcgtcca ccaccttgca ctgcagatcc
     1321 gtcaggtacc acttggccat cgaggcggtc gcggtatcga gcgtgccgtc catgacctgc
     1381 atgatgcagc ggtcgatgaa agtgcgcgct accaccgcca gcgtcttgat ctccgcgagc
     1441 ttgaagcgcg tgttctgcat ctctatcagc ttctggccga acgcgctgcg ctcgcgcgcg
     1501 tagtcgaccg tcaaccgcag cgcgcgttcg atcgccgcca ccgcgccgat gccgacgagc
     1561 gtgcgctcat agggcagttc cgccatcagt tgggcaaagc ccctgccctc gacgccgccg
     1621 agcaccgcat cgccaggcac gcgcacgttg tcgaagaaga gctcgcaggt gtcctgcccc
     1681 ttttgcccga ccttttcgag aatgcggccg acgcggaaac cctcgacgtt ccttgtctcg
     1741 accagcagca gcgagaggcc ccgcgcgccg gccgacgcat cggtcctcgc gacaacaacg
     1801 atcaggtccg cgaggtagcc gttggagatg aaggtcttcg agccgttgat gacgtagccg
     1861 ctgtcgcccc tctctgcgcg cgtgcgcacg ccctgcagat cggagccggc ggccggttcg
     1921 gacatcgcga tcgcccccac catcgcgccg ctggcaagct gcggcaggta cttgcgcttc
     1981 tgctcctcgg tgccttggtt cagcaggtag tgcgcgacga tgatgtgcac gtgcacgccg
     2041 aacgaggtgt cgccggccgc ggcgagttc
//
LOCUS       DATIRT010000155         4868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_807228, whole genome shotgun sequence.
ACCESSION   DATIRT010000155 DATIRT010000000
VERSION     DATIRT010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807228"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..777)
                     /gene="rpsB"
                     /locus_tag="VLN25_03705"
     CDS             complement(25..777)
                     /gene="rpsB"
                     /locus_tag="VLN25_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HSQ09709.1"
                     /translation="MSVTMRQMLEAGVHFGHQTRFWNPKMAPYIFGHRNKIHIINLER
                     TLEQFNDATRFVRQLAANRGTLLFVGTKRQAREIIVEEANRVSMPFVDQRWLGGMLTN
                     FKTVKVSIKRLKEMEQIIVDNGTERMTKKEALDFAREAEKLNKSIGGIKDMNALPDAL
                     FVIDVGYHKIAVQEAAKLGIPVVAVVDTNHSPEGVAYVIPGNDDSSRAVRLYARGIAD
                     AVLEGKAAALQGVVQAVTGGGEEFVEVSEGQE"
     gene            1108..1950
                     /gene="map"
                     /locus_tag="VLN25_03710"
     CDS             1108..1950
                     /gene="map"
                     /locus_tag="VLN25_03710"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HSQ09710.1"
                     /translation="MPIVIKTPTDIAGMRIAGRLAAEVLDYIAPFIKAGVTTGELDRL
                     CHEFMVNVQGTTPAPLNYAPPGYRPFPKAICTSVNHQVCHGIPGDRVLKNGDVVNLDV
                     TVIKDSYHGDTSRMYFVGEPSIASRRLCEVTYDCMWLGIEQVRPGATIGDIGHAIQTH
                     AEKHGYSVVREFCGHGIGRKFHEEPQILHYGKPGSLEKLVAGMTFTIEPMINAGRREI
                     KELADGWTIVTKDHSLSAQWEHTVLVTESGYEILTRSKATPAPPQFVVGGWRGSSVPE
                     TATA"
     gene            1955..4510
                     /locus_tag="VLN25_03715"
                     /pseudo
     CDS             1955..4510
                     /locus_tag="VLN25_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141080.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
     gene            complement(4668..>4868)
                     /locus_tag="VLN25_03720"
     CDS             complement(4668..>4868)
                     /locus_tag="VLN25_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HSQ09711.1"
                     /translation="YVRLRYRGVDLDGRPIERVADGFHARVVQHECDHLDGILYPMRM
                     RDITRFGYTDVLFPEMAEQPDD"
BASE COUNT          855 a         1626 c         1519 g          868 t
ORIGIN      
        1 ttcgccgcac gattcagcgc aaagctattc ctgcccttcg ctgacctcga caaactcctc
       61 gccgcccccc gtcacggcct gcaccactcc ttgcagtgcc gcggccttgc cttcgagaac
      121 ggcgtcggca atgccgcgcg cataaaggcg caccgcccga ctcgagtcgt cattgcccgg
      181 aatcacatag gcgacaccct cgggggagtg attggtgtcg accacagcca ccaccgggat
      241 gcccagtttg gcggcttcct gcaccgcaat cttgtgatag ccgacgtcga tgacgaacag
      301 cgcatcgggc agcgcattca tgtccttgat gccgccgatc gacttgttca gcttctcggc
      361 ctcgcgcgcg aagtccagcg cttccttctt ggtcatgcgc tcggtgccgt tgtcgacaat
      421 gatctgttcc atctctttga gacgcttgat cgagaccttg acggtcttga agttggtcaa
      481 catgccgccg agccagcgct ggtcgacaaa gggcatgctg acgcggttgg cttcctcgac
      541 gatgatctcg cgcgcctgac gtttggtgcc gacgaacaga agcgtgccac gattggccgc
      601 gagctggcgc acgaagcgcg tagcgtcgtt aaactgctcg agcgtgcgct cgagattgat
      661 gatgtggatt ttgttgcggt gaccgaagat gtacggtgcc atcttcgggt tccagaagcg
      721 ggtctggtga ccgaaatgaa ctccggcctc cagcatttga cgcatggtca cagacatgac
      781 gactctctcc aaggttgggt ccagcgcagt cgccgacgca gtcttgggtc gcttgcgacc
      841 cggaccgcac cttggtggcg gtgcgcgcga tttctgcctc cagcgttccg agcggaccgg
      901 aaccggcgcc gttgtcggct ggccggtctt gcctatctgc ccatcaaaat cccggcagat
      961 accgcaatgg caccggattg gccgtttacg ccggattccg tagcctgtga ggcacctgcc
     1021 taagcttttg attttcaaag cactgtgcag cccgatatta tagccacctc tgccagctcc
     1081 gctcaagccg cccggcctgc cccgaccatg cccatcgtga tcaagacgcc gaccgacatc
     1141 gccgggatgc gcatcgccgg ccggctcgcc gcggaggttc tcgattacat cgcgcctttc
     1201 atcaaggcag gggtcaccac cggtgaactc gaccgcctgt gccacgaatt catggtgaac
     1261 gtccagggca cgaccccggc gcccctgaat tacgcgccgc cggggtatcg gcctttcccc
     1321 aaggcgattt gcacctccgt caaccatcag gtgtgccacg gcattcccgg cgaccgcgtg
     1381 ctgaaaaacg gcgatgtcgt caacctcgac gtgaccgtca tcaaggacag ctaccacggc
     1441 gacaccagcc gaatgtactt cgtcggcgaa ccgtcgattg cgtcgcgccg cctgtgcgag
     1501 gtgacctacg actgcatgtg gctgggtatc gagcaggttc gtccgggagc caccatcggc
     1561 gacatcgggc acgcgatcca gacccacgcg gaaaagcacg gctatagcgt cgtgcgtgag
     1621 ttctgcggcc acggcatcgg ccgtaagttc cacgaagagc cgcagatcct gcattacggc
     1681 aagcccggct cattggaaaa actggtcgcc ggcatgacat tcaccatcga gccgatgatc
     1741 aatgcgggcc ggcgcgagat caaggaactc gccgacggct ggactatcgt gaccaaggat
     1801 cacagtctgt cggcccagtg ggagcacacc gtgctcgtga cagaaagcgg ctacgagatt
     1861 ctcacccgct ccaaggcgac gccagcgccc ccgcagttcg tcgtcggcgg atggcgcggt
     1921 tcttcggtac ccgagaccgc caccgcgtga ctgcatgaac gatgtcagtt tccagctgcg
     1981 ggatcgcctg cgactggaac gcgatgcact gatcgcacgc tttgaaggcg gcggcccggt
     2041 cgaggcgctg ctcaagggcc tgtcgcgttc tgccgatgcc ctcttgcgcg aacttgcacg
     2101 cacgaccggg ctggaacgcc atgccgccct ggtggcggtc ggtggctacg ggcgcggcga
     2161 gctgttcccg cattccgacg tcgacgtgct gatcctgctt gaggccgcgc ccagcgaggc
     2221 ggaacgcgcc acgatcgaaa acctgatcag cctgctctgg gatctcggcc tgacgctcgg
     2281 ccacagcgta cggacggtcg atgagtgcca gtcggaggcg cgcggcgaca tcacggtact
     2341 gacctccttg ctggaagccc ggctgatcgc cggtccgcgc cggctgttcg atcggttacg
     2401 ccgcgcagtg ctggacgtcc tcgatccagc gtcctatttt cgcgccaaag tgctggaaca
     2461 gcgccagcgc tacatcaagt tcaacgaatc cccgtacagc ctcgagccca acgtcaagga
     2521 aagcccgggc ggcctgcgcg acctgcacgt gcttctgtgg atcgcccgcg ctggcggttt
     2581 cggaacgacc tggaccgtgc tcgcgcggcg cggactgatc acgccggaag aggcgggcct
     2641 gatccgcctg agcgagcgcc ggctgaagca ggtccgcgcc tggctgcacc tgatcaccgg
     2701 ccgccgcgaa gaccggctcg tcttcgacgt gcagcaggcc gtcgcgcagc gcgcgggctt
     2761 ccagccgacc gccgcgcggc gcgcgagcga ggtgctgatg cagcgctatt actgggcagc
     2821 caaagcggtc acccagatga acacgatcgt gctgcagaac atcgagctgc gcctgtttca
     2881 ccgcgccgac gagccggcgc agccgctgga cgagaccttc gtgacgcgca gtgaactgct
     2941 cgacctgcgt gcgcccgacg ccctggaggc tgaccccaac ggcatcctgc gcgccttcct
     3001 gctgatggcg cagcaccagg agttgcgcgg catgtccgcc ccgctgatgc gcgcgctctg
     3061 gcatgcgcgc gtgcgcatcg acgcgcgcta ccggcgcgac ccggccaacc gcgcaacctt
     3121 cctcgcgatc ctgcagcagc cgaagggcgt gctgcacgaa ctgcgccgca tgaaccagct
     3181 gtccgtgctc ggtcgctatc tgccggtgtt ccgtcgcatc gtcgggcaga tgcagcatga
     3241 tcttttccac gtctacacgg ttgatcagca catcctgcag gtgctcagga agttgcgccg
     3301 atttgcgctc gccgagcatg cgcatgaatt cccgctgtgc agccagctga tcgcggactt
     3361 cgataaaccc tggctgctgt acgtcgctgc actcttccat gacatcgcca agggacgcgg
     3421 cggcgatcac agcgacctgg gagcccgcga cgcgcgacgc ttctgtcgcg aacatggact
     3481 gtccgccgac gacacggcgc tggtcgtgtt cctggtcgaa catcatctgt cgatgtcgca
     3541 ggtggcgcag aagcaggacc tcgccgacga ggaggtgatc gggcgcttcg ccaggcatgt
     3601 gcagacctag cgccggctga ttgcgctcta cctgctcacc gtcgcggata tccgtggcac
     3661 cagccccaag gtgtggaacg cgtggaaggg caagttgctc gaggatctgt tccaggctac
     3721 gcgacggttg ttgggaggag gcagcgagtc gccgaccgcc gtgctcgatg gtcgcaagga
     3781 ggaggccctg cgcatcctga ggctgtacgg cctgtccgac agtgcgcgcg aagcgctctg
     3841 ggagcagctc gacgtcgtgt acttcctgcg tcacagcgcg caggacatcg cctggcacac
     3901 gcggtcgctc ttctcccgtg tgcacaccga cccgccagtg gttcgcgcgc gcttggcgcg
     3961 cattggcgaa ggcgccgagg tactgatcta cgtgaaggac cagaaggacc tgtttgcccg
     4021 cgtctgtggc tacttcgaaa gtcgcaacct gagcattctc gacgcgcgca tccacacgac
     4081 ccggcacggt tatgcgctcg acacgtttct cgtcaccgac aacggccacg cgccccacta
     4141 ccgtgaactg ctggcgcagg tcgagcacga actgggcgaa tggattgccc ggcagcacaa
     4201 attgccgacg ccggtcaaag gccgcatgtc acgccagtcg cgccacttcc cggtgacccc
     4261 tgaagtacga ctgcagcccg acgagcgggg gtcgcgctat ctgctgtcag tcacggcgac
     4321 cgaccgcctg ggactgcttt actcgatcgc gcgcgtgctg acccaacacc acttgaacgt
     4381 gcacacggcg aaggtgctta cgctgggcga acgcgccgag gacgtgtttc tcgtctcggg
     4441 cgcggaactc gaccaggcgc gcagtcagct cgagatcgag aaggacctgc tcgaagcgct
     4501 ggcgccttga cggcgccgct cacgtacgca accgcttcgc ggtcgcacgt gcgctcgcgg
     4561 cggatacgac gggctatgcc cgccgctcaa gactagtcag tcggctgcgc tccggagcgg
     4621 ccgcgcgctc cgccgaaaag caaagggcag gctgggcggc agtccttcta gtcgtccggc
     4681 tgttcggcca tctccggaaa caggacgtcg gtgtagccga agcgagtgat gtcgcgcatg
     4741 cgcatcggat acaggatgcc gtcgaggtga tcgcactcat gctgaaccac gcgcgcgtgg
     4801 aagccatcgg ccacccgctc gatcggtcgg ccatccaggt cgaccccccg gtaccgcagt
     4861 cgaacata
//
LOCUS       DATIRT010000156         2510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1005353, whole genome shotgun sequence.
ACCESSION   DATIRT010000156 DATIRT010000000
VERSION     DATIRT010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005353"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VLN25_03725"
     CDS             complement(<1..196)
                     /locus_tag="VLN25_03725"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HSQ09712.1"
                     /translation="MDKVQMLIGLSIALFAIGLTGVIIRRNVLVMLMCMELMLNGVNL
                     SLVTFAAQSGSAVGAVLVFLV"
     gene            complement(205..729)
                     /locus_tag="VLN25_03730"
     CDS             complement(205..729)
                     /locus_tag="VLN25_03730"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HSQ09713.1"
                     /translation="MGTLLANLDTLLLVVFAACALIGAALMLVLREPMRVALALISTM
                     VFLGGIYGLLGVHFIAAFQVLIYVGAVMVFIVYVIMLLDPRDSAVRRRFSGLLIPGVI
                     GCGLFIWALGALVWDGAGAVKPGSGGAGFTLAQFSAAFLNEYWLEFELTSVLLVAAIV
                     AAIAVIQVSRKQHG"
     gene            complement(729..1310)
                     /locus_tag="VLN25_03735"
     CDS             complement(729..1310)
                     /locus_tag="VLN25_03735"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HSQ09714.1"
                     /translation="MARKPYVRKQYWNQPTMSWWEKAYFFEIVRGLLITGGVFSRNML
                     RWMTGRKGALTTYYPEEKRWDYAKGNRGKHVLTQREDGSGRPQCIACNMCATVCPAKV
                     IEIEAAFDPADTVHPKYPARFEIDYSRCVFCGLCVEACPEDAIRMVEDVPHLTSADRQ
                     HMWLGKEELLTWRPQSDVAKHYAAPVATPRGHA"
     gene            complement(1310..>2510)
                     /locus_tag="VLN25_03740"
     CDS             complement(1310..>2510)
                     /locus_tag="VLN25_03740"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HSQ09715.1"
                     /translation="WRCPCRAAVRAAGHDCRGIRMIQDLVIAIVFITYAISVLMGFGT
                     ILTWVERKQAAVMSDRIGANRAYLRIPFTQVKLVWWGLFHGMADGLKMILKEDFKPKT
                     YDWYAYAVAPWVVFTPVLLVFAVIPFGGQLVPGELIPPLADWFGDRSYPMQIARLDAG
                     LLMVFAFGGMTIIGAMLAGWSSSNKFSLLGALRAGSQMISYEVIMGLTVLGLIVIFGT
                     VDLNSIVQQQSATVLGFLPAWGIVLQPFAAILFMVAAVAENKRIPFDLPEAESELIAG
                     YYTEYSAMKMGLFMFAEFIEIAIIGALFTTLFLGGYNLPFMTDAGFEFPGGHVVAMSH
                     GAVVVTQLVVFLAKVFVVCSFQILVRWSLPRFRYDQVLRFAWKFMLPLALANLVVTVI
                     VVWGLQL"
BASE COUNT          525 a          823 c          772 g          390 t
ORIGIN      
        1 acaccaggaa cacgaggacc gcgccgacgg ccgagccgct ctgggccgcg aaggtcacca
       61 ggctgaggtt gacgccgttg agcatcagtt ccatgcacat cagcatcacc agcacattgc
      121 gccggatgat cacgccggtc agaccgatcg cgaaaagcgc gatcgacagt ccgatcagca
      181 tctgcacttt atccatgctt gcacttatcc atgctgtttt cggctgacct ggatgactgc
      241 gatcgccgcg acgatcgcgg ccaccagcag gaccgacgtc agttcgaact cgagccagta
      301 ttcattgagg aacgccgccg aaaactgggc cagcgtgaag cccgcgccgc cggagcctgg
      361 cttgaccgcg cccgcgccgt cccacacgag ggcacccagt gcccagatga acagtccgca
      421 cccgatgacg cccgggatca gcaggcctga aaagcgtcgc ctgaccgcag agtcgcgcgg
      481 gtcgagcagc atgatcacgt agacgataaa caccatgacc gcgccgacat agatcagcac
      541 ctggaacgcc gcgatgaagt gcacgccgag caggccgtag atcccgccaa ggaacaccat
      601 tgtcgaaatc agggccagcg ccacgcgcat tggctcgcgc agcacgagca tcagcgcagc
      661 gccgatcagc gcgcaggcag cgaacacgac cagcagcagc gtgtccaggt tggcgagcag
      721 cgtgcccatc aggcatgtcc ccgtggcgtc gcgaccggag ccgcgtagtg cttggcgacg
      781 tcgctctgtg gccgccaggt caggagttct tccttgccga gccacatgtg ttgccggtcg
      841 gccgaggtca gatgcggaac gtcctcgacc atgcggatcg cgtcctccgg gcaggcctcg
      901 acgcacaggc cgcagaagac acagcgtgag tagtcgatct cgaagcgagc cggatacttc
      961 gggtggaccg tatcggcagg atcgaaggcg gcctcgatct cgatcacctt ggccgggcac
     1021 acggttgcgc acatgttgca ggcgatgcac tgcggccggc cggagccatc ctcgcgttgc
     1081 gtcagcacgt gcttgccgcg gttgcccttg gcgtagtccc agcgcttttc ctcggggtag
     1141 tacgtcgtca gcgctccctt gcgcccggtc atccagcgca acatgttgcg gctgaacacg
     1201 ccaccggtga tcaggaggcc gcgcacgatc tcgaagaaat acgccttctc ccaccacgac
     1261 atggtcggct ggttccagta ctgcttgcgg acgtacggtt tgcgcgccat cagagttgca
     1321 gcccccagac gacgatcacg gtgacgacga ggttcgccag cgccagcggc agcatgaatt
     1381 tccaggcaaa gcgcagcacc tggtcatagc ggaagcgcgg cagcgaccag cgcaccagga
     1441 tctggaaact gcacaccacg aacaccttgg ccaggaatac gacaagctgg gtcaccacga
     1501 ccgctccgtg gctcatcgcc acgacgtgcc cgccggggaa ctcgaagccg gcatccgtca
     1561 tgaacggcag gttgtagcca ccgaggaaca gcgtcgtgaa gagggcgccg atgatcgcga
     1621 tctcgatgaa ctcggcaaac atgaacaggc ccattttcat cgccgagtac tcggtgtagt
     1681 agccggcgat gagttccgac tcggcctcgg gcaggtcgaa cgggatgcgc ttgttctccg
     1741 ccaccgccgc gaccatgaac aggatcgcgg cgaaaggctg cagcacgatg ccccaggcgg
     1801 ggaggaaccc cagaaccgtg gctgactgct gctgcacgat ggaattcagg tccaccgtgc
     1861 cgaagatcac gatcagcccg agcaccgtca gtcccatgat cacttcgtag gagatcatct
     1921 ggctgcccgc gcgcaaggcg ccgagcagcg agaacttgtt cgacgacgac cagcccgcca
     1981 gcatggcccc gatgatcgtc atgccgccga aggcgaacac catcagcagt ccggcatcga
     2041 gccgcgcgat ctgcatcggg tagcttcgat ccccgaacca gtcggccagt ggcggaatga
     2101 gttcgcccgg caccagctgg cctccgaacg gaatgacggc gaacaccagc agcaccggcg
     2161 taaacacgac ccagggggcg actgcgtagg cgtaccagtc gtaggtcttc ggcttgaagt
     2221 cctccttcag gatcatcttc agtccgtcgg ccataccgtg aaacagcccc caccagacga
     2281 gcttcacctg cgtgaaggga atgcgcaagt aggcgcggtt ggcgccgatg cgatcggaca
     2341 tcacggccgc ctgcttgcgc tcgacccatg tcaggatcgt gccgaagccc atcagcacgg
     2401 agatcgcata ggtgatgaac acgatcgcga tgacgaggtc ctggatcatg cgaatgcccc
     2461 ggcagtcgtg gccagctgcg cgaacagctg ctcggcatgg gcaacgccat
//
LOCUS       DATIRT010000157         2792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1103818, whole genome shotgun sequence.
ACCESSION   DATIRT010000157 DATIRT010000000
VERSION     DATIRT010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103818"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VLN25_03745"
     CDS             <1..787
                     /locus_tag="VLN25_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HSQ09716.1"
                     /translation="LEYAWDHPEVRALSHPLPPFFALPTTSGTGSEVGRSSVVSEADT
                     HVKRVIFSPQLLAREVFADPELTLGLPPAVTAATGMDALTHNIESYLSPAYHPLCDGI
                     ALEGVRIAARALPIAMRDGSNLQARADMMMASMMGAIAFQKDLGAVHSCAHALGAVCD
                     LHHGLANALMLEPVMRFNAEAVPARFAELAHVAGLDSAKDFVPWLARLKREIGIQPSL
                     TDAGVKREQIPRLVELAVKDVCHQTNPRPCTAADFERFFEQAF"
     gene            787..1623
                     /locus_tag="VLN25_03750"
     CDS             787..1623
                     /locus_tag="VLN25_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HSQ09717.1"
                     /translation="MSQRPTIGISACFFHADPSRPIFTGKTLQYVEQSIVHWVQSSGA
                     LAVVIPSPEGPTQRGDVSLADYAELLDGLVLEGGSDMWPGSYGEEPLRPQWGGDRIRD
                     EYEIALLRAFVDSGKPVLGICRGLQVINVAFGGTLFQDIQTQLPGAIEHRNAKLYDQN
                     FHTIEFVPGTRLADLYPHPGFKAPDRPHPHFVVNSVHHQSIRELAPFFSVEARCPNDG
                     VIEAIRYDASRGPIQSYVAAVQWHPEFHDWTNSSLLSGDPLLTDFIGAVRSARRDTTS
                     TC"
     gene            1617..>2792
                     /locus_tag="VLN25_03755"
     CDS             1617..>2792
                     /locus_tag="VLN25_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSQ09718.1"
                     /translation="MLTVINPANGRVIAELSEDTADAVASAYRSARAAQADWAAQPLT
                     ARIETIRRFRGELVRDLERLAAILTSEVGKPIRQSRNEISGVLGRIDFFLAESERTLA
                     SRQVFDESGMREQITHEALGVVANISAWNYPYFVGANVFVPALIAGNAVLYKPSEYAT
                     LTGLEIGKLLHISGVPQDAFKVLVGGGAVGSALLVQPVDGVFFTGSHATGAKIAQTVG
                     PRMIKLQLELGGKDPIYVCEDVDIATAAASLADGAMYNTGQSCCSVERIYVHASIHDA
                     FVDAFVSEVRGFTVSDPTDEAAYIGPLTRAPQLEVLERQVADATAKGARLLLGGKRVA
                     GPGNWFAPTVFSHVDHSMDLMREETFGPVIGIQRVADDGEAVRLMNHTEYGLTAGVYT
                     "
BASE COUNT          481 a          945 c          893 g          473 t
ORIGIN      
        1 cctcgaatac gcgtgggacc acccggaggt gcgcgcgctg tcgcacccac taccgccctt
       61 cttcgcgctg ccgaccacca gcggcaccgg ctcggaagtc ggccgctcga gcgtcgtcag
      121 cgaggccgac acgcacgtta agcgggtgat cttctcgccc cagctgctgg cgcgcgaagt
      181 ctttgcggat ccggaactca cgctcgggct gccaccggcg gtgactgccg caactggcat
      241 ggacgcgctg acgcacaaca tcgagagtta tctgtcaccg gcgtaccacc cgttgtgcga
      301 cggcatcgcg ctcgaaggtg tacgcatcgc cgcgcgcgcg ctgccgatcg cgatgcgcga
      361 cggcagcaat ctgcaggcgc gcgccgacat gatgatggcc tcgatgatgg gggcgatcgc
      421 gttccagaag gacctcggtg cagtgcactc gtgcgcgcat gcgctcggtg ccgtgtgcga
      481 cctgcaccat ggactcgcca atgcgctgat gctcgaaccc gtgatgcgct tcaacgccga
      541 agcggtgcct gccagattcg cggaactggc gcatgtcgcc ggcctggatt ccgcgaaaga
      601 cttcgtgccg tggctcgcac ggctgaagcg cgagatcggc atccaaccat cgctgactga
      661 tgccggcgtg aagcgggagc aaattccgcg gctggtcgag ctggcagtca aggacgtctg
      721 ccaccagaca aatcctcggc cgtgcactgc cgccgatttc gagcgcttct tcgagcaggc
      781 cttctgatga gccagcgacc gaccatcggt atttcggcgt gcttcttcca cgccgatcca
      841 tcgcgaccga tcttcaccgg caagacgctg cagtacgtcg agcagtcgat cgttcattgg
      901 gtgcagtcgt ccggcgcgct ggcggtcgtg attccgtcgc ccgaggggcc gacacagcgc
      961 ggcgatgtct cgctcgccga ctatgcggaa ctgctcgacg gactcgtgct cgaaggcggc
     1021 tccgacatgt ggccgggcag ctacggtgag gagccgctgc gaccccagtg gggcggcgat
     1081 cgcatccgcg acgagtacga gatcgcgctg ctgcgcgctt ttgtcgattc gggcaagccg
     1141 gtgctcggca tctgccgcgg cctgcaggta atcaacgtcg ccttcggcgg aacactcttc
     1201 caggacatcc agacccagct ccccggcgcc atcgaacacc gcaatgccaa gctctatgac
     1261 cagaacttcc acacaatcga gttcgtgccg gggacgcgac tggcggatct gtatccccat
     1321 ccggggttca aggcgcccga ccggccgcac ccccacttcg tggtcaacag cgttcatcac
     1381 cagagcatcc gcgagctggc gccgttcttc agcgtcgaag cgcgctgccc gaacgacggc
     1441 gtgatcgagg caatccgtta cgacgcttcg cgcggcccga tccagtcgta tgtcgcggcg
     1501 gtgcagtggc atccggaatt ccacgactgg accaactcat ctctgctgtc cggcgacccc
     1561 ctgctcaccg acttcatcgg cgccgttcgc agcgcacggc gcgacaccac ctcgacatgc
     1621 tgactgtcat caatcccgcg aacggccgcg tcatcgccga actttccgag gacacggcgg
     1681 acgcggtcgc ctccgcctac cgaagtgcgc gcgccgccca ggcagactgg gccgcgcagc
     1741 cgctgacagc acgcatcgag acgattcgcc gctttcgcgg cgaactggtg cgcgatctcg
     1801 agcgactcgc cgcgatactg acctcggaag tcggcaagcc gatccggcag tcccgcaacg
     1861 aaatcagtgg cgtgctcggc cgcatcgact tcttcctcgc agagtccgaa cgcactctgg
     1921 cttcgcgcca ggtcttcgac gaaagcggca tgcgcgaaca gatcacgcac gaagcgttgg
     1981 gcgtggtcgc gaacatctcg gcgtggaact atccctactt cgtcggcgcc aatgtcttcg
     2041 tgcccgcgct gatcgccggc aacgcggtgc tctacaagcc ctccgagtac gcgacgctga
     2101 cggggctcga gatcggcaag ctgctgcaca tctcgggcgt cccccaggac gcgttcaagg
     2161 tgctggtcgg cggcggtgcg gtgggttcgg ccctgctggt gcagcccgtc gatggtgtgt
     2221 tcttcaccgg ttcgcatgcc accggcgcga agatcgcgca gacagtcggc ccgcgcatga
     2281 tcaagctgca gctcgaactc ggcggcaagg atcccatcta cgtctgcgag gacgtcgaca
     2341 tcgcgacggc ggcggcgtcg ctcgccgatg gcgcgatgta caacaccggg cagagctgct
     2401 gctcggtcga gcgcatctac gtgcatgcgt caatccacga tgcgttcgtc gacgccttcg
     2461 tcagcgaggt gcgcggcttc acagtcagcg atccgaccga cgaggccgct tacatcggcc
     2521 cactgacgcg tgctccgcaa ctcgaagtgc tcgagcgcca ggtcgccgac gcgaccgcaa
     2581 agggggcgag gctgctgctc ggcggaaagc gagtggcagg accgggcaac tggttcgcgc
     2641 cgacggtgtt cagtcacgtc gatcactcaa tggacctgat gcgcgaggag accttcggcc
     2701 cggtcatcgg catccagagg gtggcggatg acggcgaagc ggtccggctg atgaatcaca
     2761 ccgagtacgg gctgacggcg ggggtctaca ca
//
LOCUS       DATIRT010000158         3447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1595498, whole genome shotgun sequence.
ACCESSION   DATIRT010000158 DATIRT010000000
VERSION     DATIRT010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595498"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..2325
                     /locus_tag="VLN25_03760"
     CDS             124..2325
                     /locus_tag="VLN25_03760"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSQ09719.1"
                     /translation="MAGAMRLGELVHDMETRMEAAMQLSDVPGIIIEDLQGQYDQATA
                     LFEELQNPDAAKPSAPAPAEPEPAKSDEPALAPVIDLATARGEPKLEPRVEKPAALPS
                     TVEATLAATAPTQQAAPFIRVRADVLDKLVDQAGEVSIARSKLENEVSTIKGSLTDLT
                     ENIQRLRSQLREVEIQAEAQVQARGASLSRESADFDPLEFDRYTRLQELTRMLAESVE
                     DVAMVQSNMVKGLQMADTDLTSQSRLTRDLQQQLMRVRLVPFANVSERLYRVARQTAK
                     ELDKRVNVDVRGGTTEIDRGVLEKMVGPFEHLIRNAIVHGLELPAERRAAGKAEAGEL
                     VLDIRQEANEIIVVLSDDGAGLNLERIRERAIERELISADQVLGDRELMDLIFLPGFS
                     TATEVTELAGRGVGLDVVRAELASFGGRIAVSSESGRGTRFTLYLPMTLAVAQVVLAR
                     IGTRRYALPAGMVEQVRRYRPIMLLPSLAEGMIDIPPAGPVVLRPLSQLVGEETSGHL
                     SKQTPVILLRSGDDRMAIAVDDVTSNQEVVVKNVGAQVARLAGILGATILGNGEIVLI
                     INPVQLITRAPEPPAIFEEELRAETKAAPNRTVPATEIGVATMPTVMVVDDSLTVRRV
                     TQRLLERNNFIVLLAKDGVDALRQLQDTKPDVMLVDIEMPRMDGYDLTLNVRSSPVTS
                     SIPIIMITSRTAEKHRSMAFELGVNEYLGKPYQEDELVKLVRHYVGDRVTA"
     gene            complement(2673..>3447)
                     /locus_tag="VLN25_03765"
     CDS             complement(2673..>3447)
                     /locus_tag="VLN25_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HSQ09720.1"
                     /translation="LTRIDRYDEVRNFPAVRGPSYLSVHLRFGTLSVRELFRTARSVA
                     SSGASTWLSELIWRDFYQMILWHHPHVAERAFRPEYDAIEWDTGPAADAAFAAWCNGR
                     TGYPLVDAAMAQLNQTGYMHNRLRMIVASFLTKDLGIDWRRGEHYFAQHLNDFDLAAN
                     NGGWQWAASTGCDAQPYFRIFNPVTQSEKFDPDGTFIRRYLPQLASFSAKEIHAPWLA
                     SADRQREAGCVIGVDYPAPVVDHAQARKRTLERYSVVKR"
BASE COUNT          627 a         1122 c         1154 g          544 t
ORIGIN      
        1 ctacaagcgc gacatcgacg acatccgcga atcgccgtcg ctggacgtgc tgacgctgct
       61 ccaccagaaa ggtgccgtcg tccgctacgc cgatccgtac gtctccagtc tggcagcgcc
      121 cgcatggccg gcgcgatgcg gctgggcgaa ctcgtgcacg acatggaaac gcgcatggaa
      181 gccgccatgc agttgtccga cgtgcccggc atcatcatcg aggacctgca gggccagtac
      241 gaccaggcga ccgccctgtt cgaggaactt cagaatcccg acgccgcgaa gccgtccgcc
      301 ccggcgcccg ccgagccgga accggctaag tccgatgaac ccgcgctcgc gccggtcatc
      361 gacctggcta ccgcgcgcgg cgagcccaag ctcgagccgc gtgtcgagaa gcccgctgcc
      421 ttgccgtcga ccgtcgaggc gacgctggcg gcaacggccc cgacgcagca ggcggcaccg
      481 ttcatccgcg tgcgcgccga cgtgctcgac aagctggtcg accaggccgg cgaagtctcg
      541 atcgcccgct ccaagctcga aaacgaggta tcgacgatca agggatcgct gacggaccta
      601 accgagaaca tccagcgact gcgctcgcag ttgcgcgaag tcgagatcca ggccgaagcc
      661 caggtgcagg cgcgcggcgc atcgctgtcg cgtgaatcgg cggacttcga cccgctcgag
      721 ttcgaccgct atacgcggct gcaggaactg acgcgcatgc tggccgagtc ggtcgaagac
      781 gtggcgatgg tccagagcaa catggtgaag ggcctgcaga tggccgacac cgacctgacc
      841 tcgcagtcgc ggctcacgcg ggacctgcag cagcaactga tgcgcgtgcg actggtgcca
      901 tttgccaacg tgtccgagcg gctctatcgc gtcgcgcggc agaccgccaa ggaactcgac
      961 aagcgggtca acgtcgacgt acgcggcggc acgaccgaaa tcgaccgcgg cgtactcgag
     1021 aagatggtcg ggccgttcga gcatctgatc cgcaacgcga tcgtgcacgg actcgaactg
     1081 cccgccgagc gacgtgcggc gggcaaggcc gaagccggcg aactcgtgct cgacattcgc
     1141 caggaagcca atgaaatcat cgttgtcctc agcgacgacg gtgccggcct gaatctcgag
     1201 cggatacgcg agcgtgcgat cgagcgcgaa ctcatcagtg ccgaccaggt gcttggcgat
     1261 cgcgagttga tggacctcat cttcctgccg ggattctcga ccgccacgga agtcacggaa
     1321 ctggctggac gcggtgtcgg cttggacgtc gtacgggcgg agcttgcgtc gttcggcggc
     1381 cgtatcgccg tgtcgagcga gtccgggcgc ggcacgcgat tcacgcttta cctgccgatg
     1441 acgctggccg tcgcacaggt cgtgctggcg cgcatcggca cgcgccgcta tgcgctgccg
     1501 gccggcatgg tcgagcaggt gcggcgctac cggccgatca tgctgctgcc gtcgctggct
     1561 gaaggcatga tcgacatccc gccggccggt ccggtcgtat tgcggccgct gtcgcagctg
     1621 gtgggcgaag agaccagcgg acacctgtcg aaacagacgc cggtcatcct gctgcgctcg
     1681 ggcgacgatc gcatggccat cgcggtcgac gatgtgacga gtaaccagga agtggtggtc
     1741 aagaacgtcg gcgcgcaggt cgcccggctc gccggcatcc tgggcgcgac catcctgggt
     1801 aacggcgaga tcgtgctgat catcaatccg gtgcagttga tcactcgcgc gcccgagccg
     1861 cccgcgatct tcgaggagga gctgcgcgcc gagaccaagg cggcaccgaa ccgcaccgta
     1921 cctgcgaccg agattggcgt ggccaccatg ccgaccgtga tggtcgtcga cgactcgctg
     1981 accgtgcgcc gtgtcacgca gcgacttctc gaacgtaaca atttcatcgt gctgctcgcg
     2041 aaggacggcg tcgatgcgct gcggcagctg caggacacca agcccgatgt gatgctggtc
     2101 gacatcgaaa tgccgcgcat ggacggctac gacctgacgc tcaatgtccg cagttcgccg
     2161 gtcacctcct cgatcccgat catcatgatc acttcgcgta ccgccgagaa acatcgcagc
     2221 atggcgttcg agctgggagt caacgagtac ctcggcaagc cgtatcagga agacgagctg
     2281 gtgaagctcg tcaggcacta cgtgggagat cgggttacgg cgtagcgcga actgcgcagc
     2341 atagggtgcc gctgcgcgtg ttgcgatcct gtcgtgccac gaccgagccg ggagtcgccc
     2401 ggcggcgagt gactttcttt gtctcgccaa agaaagtcac caaagaaagg cgaccgcgcg
     2461 aggaaggcct acggctcccc ttgcgctgca aggcgtcagc gggccaggcg cgaaactcgc
     2521 tccctgcagt cgctcagaca cgcgcccggc acttctccgc tgccgccccg caccgctacg
     2581 gcgtccccga gcggatgacc acctgcggcg taccagtgcc ggcggagaac gtgcaatgtg
     2641 cccagcgtta aaggcccgaa agccgcacat tcctagcgct tcaccactga gtaacgctcc
     2701 agcgtgcgct tgcgggcctg agcatggtcc acgactggag cggggtagtc gacaccgatc
     2761 acgcatcccg cctcgcgctg gcgatcggcg ctggcgagcc atggcgcgtg gatctccttc
     2821 gcgctgaagc tcgccaactg cggcaggtag cgccggatga acgtgccgtc gggatcgaac
     2881 ttctccgact gcgtgaccgg attgaagatg cggaaatacg gttgcgcgtc gcagccggtg
     2941 gacgctgccc actgccagcc gccgttgttg gctgcgaggt cgaagtcgtt caggtgctgc
     3001 gcgaagtagt gttcgccgcg gcgccagtcg atgccgaggt ccttggtgag gaagctggcc
     3061 acgatcatgc gcagccggtt gtgcatgtag ccggtctggt tgagctgcgc catcgcagcg
     3121 tcgaccagcg gatagccggt acgcccgttg caccaggcgg cgaacgccgc atcggccgcg
     3181 gggccggtgt cccattcgat cgcgtcgtac tcgggtcgga aggcccgttc ggccacgtgc
     3241 gggtgatgcc acaggatcat ctggtagaag tcgcgccaga tgagttccga cagccaggtc
     3301 gatgcgcccg aggatgcgac ggagcgggcg gtgcggaaga gttcgcgcac ggacaacgtg
     3361 ccgaagcgca ggtgcactga aagatatgac ggcccgcgca cggcgggaaa gttgcgcacc
     3421 tcgtcatagc gatcgatgcg cgtcagg
//
LOCUS       DATIRT010000159         5107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_907419, whole genome shotgun sequence.
ACCESSION   DATIRT010000159 DATIRT010000000
VERSION     DATIRT010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907419"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /gene="def"
                     /locus_tag="VLN25_03770"
     CDS             complement(<1..486)
                     /gene="def"
                     /locus_tag="VLN25_03770"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HSQ09721.1"
                     /translation="MIREILRMGDPRLLRVSEPVRDFGTPELRTLVADMFDTMHAANG
                     AGLAAPQIGVLKRVVIFGYADPAMRNPRYPDADPVPQTILLNPVLEPLSESTEEGWEG
                     CLSVPGLRGVVDRYVRLRYRGVDLDGRPIERVADGFHARVVQHECDHLDGILYPMRMR
                     DI"
     gene            complement(483..1226)
                     /locus_tag="VLN25_03775"
     CDS             complement(483..1226)
                     /locus_tag="VLN25_03775"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HSQ09722.1"
                     /translation="MYAIIGGSGLAKLSALENLRRQVMRTPYGEPSGALTFGRFAGAE
                     VVFLARHGYGHTLAPHEINYRANLWALRELKVEGVISVATVGGIRADLGPGALVVPDQ
                     IIDYTHSRKGTFFEGSEMPVMHVDFTQPYTPALRARLLAAAVMCGEPVSDGGVYACTQ
                     GPRLETAAEIERIAREGGEMVGMTGMPEAALAREAELAYATLAVVVNHAAGRGDSKLA
                     VRLEELEAVMRATMTRAVRIMAAFFAASQ"
     gene            complement(1226..3262)
                     /gene="ligA"
                     /locus_tag="VLN25_03780"
     CDS             complement(1226..3262)
                     /gene="ligA"
                     /locus_tag="VLN25_03780"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HSQ09723.1"
                     /translation="MPSARHDSARQRARRLRDLIGRADHDYYVLDAPSIPDAEYDRLF
                     RELQELEAAHPELVVSESPTQRVGGAVRSDLPKVRHVVPMLSIRTETDAGPSGARAFD
                     ARVRRELGLAADDPPIEYCAELKFDGLAVSLRYERGVLVRAATRGDGEEGEDVTPNAR
                     TIRAIPLRLNGVDALVLEVRGEIFLRRRDFEQINERQRETGDKVFVNPRNAAAGFLRQ
                     LDAGVTASRPLSFYAYGLGEVAGWTLPPTHSAMLHALANMGVPVAAERRVASGADELV
                     AFHADIAERRDRLPFDIDGVVYKVNSLELQRRLGFVSREPRWAVAHKYPAQEELTQVT
                     GIDVQVGRTGKITPVARLAPVFVGGVTVSNATLHNEDYIRSLGLMIGDTVIVRRAGDV
                     IPQVVSVLPDRRPKNARNFAMPQACPVCGSSVAREEGEKDHRCTGGLFCAAQRKQALL
                     HFAGRRALDIEGLGDKLVEQLVDAGLVQTPADLFKLGLGALAGLERMADKSAANVLAA
                     LERARSTTLERFIYALGIRHVGESTARDLARHFGSLDAVMDASQEELLQVTDVGPVVA
                     ASIARFFAEPHNREVVEQLRAAGVHWTEAAPHRAPAGALAGLTFVLTGTLRHWTRDEA
                     KDRIEAAGGKVAGSVSKKTDFVVAGEDAGSKLDKALQLGVPLIDEARLKTMLEG"
     gene            complement(3357..4502)
                     /locus_tag="VLN25_03785"
     CDS             complement(3357..4502)
                     /locus_tag="VLN25_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09724.1"
                     /translation="MTDLHWALLTIAGVLLLAIWIYSKWQERRALARLDAAMRQGVGD
                     PLLGTRPLPSPAASSRRIEPRLDGAGGVGNDAATASQETAPEAERTAVEVADQSADEG
                     AEVADERSEPSAYALPDGWSEDPLLDFVIELRCAHAIDGVAALEAKGQLERLQLPLPT
                     HLAVWDAKTQQWTGPDRFGFYSELLIAVQMAHRNGVLGEIDAARFVAATQQIALGIDA
                     DFDAPDVPRLVAQAAELDRVCARFDVQVTLTLESGETPWTADVIQAAAIPVGFTAVGS
                     GRWEMREASGELALSMTAGALPANRLAIFIDVPLVRPEGSPLARQFAVADQLATRLNA
                     RVVDDNGKPVQREAHFAIAAQLAGMYEQMQAAGIAAGSERARRLYAV"
     gene            complement(4509..>5107)
                     /locus_tag="VLN25_03790"
     CDS             complement(4509..>5107)
                     /locus_tag="VLN25_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSQ09725.1"
                     /translation="LEELTTSRERKSFLDAQSADLKAAITTLEDAIRKIDRESREMLQ
                     QTFDQVNAHFGQLFPKLFGGGEARLIMTGEEILDAGVQVMAQPPGKRNSSIHLLSGGE
                     KALTATALVLALFKLNPAPFCLLDEVDAPLDDANTERFCRLVESMTVNTQFLFITHNK
                     IAMEMAQQLIGVTMQEQGVSRIVAVDIEGAVRLTAEAA"
BASE COUNT          875 a         1789 c         1596 g          847 t
ORIGIN      
        1 gatgtcgcgc atgcgcatcg gatacaggat gccgtcgagg tgatcgcact catgctgaac
       61 cacgcgcgcg tggaagccat cggccacccg ctcgatcggt cggccatcca ggtcgacccc
      121 ccggtaccgc agtcgaacat accggtcgac gacgccgcgc agcccaggca ccgacaggca
      181 accttcccat ccctcctcgg tcgactccga caggggttcc aggaccgggt tgagcaggat
      241 cgtctgaggc accggatccg cgtccgggta acgcggattg cgcattgccg ggtccgcata
      301 gccgaagata accacccgct tcagaactcc gatctgcggt gctgccagtc ccgcaccgtt
      361 cgccgcatgc atcgtgtcga acatgtccgc caccagcgtg cgcaattcgg gcgtgccgaa
      421 atcccgcacg ggctcggaca cacgaagcag gcgcgggtcc cccatgcgaa ggatctcgcg
      481 gatcattgcg acgctgcgaa gaacgccgcc atgatccgaa ccgcccgcgt catcgtggca
      541 cgcatgaccg cttccagttc ctcgagcctg acggcgagtt tgctgtcgcc ccggccggcc
      601 gcatggttca cgaccacggc gagcgtggca taggcaagct cagcctcgcg cgcaagcgcg
      661 gcctccggca tcccggtcat gcccaccatt tccccacctt cgcgcgcgat gcgctcgatc
      721 tccgcggcgg tttccagccg cggtccctga gtgcacgcat agacgccgcc gtccgagacc
      781 ggttcgccgc acatcacggc cgccgccagc agtcgcgccc gcagggccgg cgtatacggt
      841 tgcgtgaaat cgacgtgcat gactggcatc tccgaaccct cgaagaaggt gcctttgcga
      901 ctgtgcgtgt agtcgatgat ctggtcaggt accaccaggg cgccgggtcc gagatcggcg
      961 cgaatgccgc cgacggtcgc caccgagatc acgccttcga ccttcagctc gcgcagcgcc
     1021 cataggttgg cgcggtagtt gatctcgtgt ggagcgagcg tatgaccgta accatggcgc
     1081 gccaggaaca ccacttcggc gccggcgaag cgcccgaagg tcagcgcgcc cgagggctcg
     1141 ccgtacggcg tgcgcatcac ctgccggcgc aggttctcca gcgccgagag tttcgccagt
     1201 ccacttccac caatgatcgc gtacatcagc cttccagcat ggttttcaag cgggcctcgt
     1261 cgatgagcgg cacgccgagc tgcagtgcct tgtccagctt gctgccggcg tcctcgcccg
     1321 cgacgacgaa gtcggtcttc ttcgacacgg agcccgcgac tttgccgcca gcggcttcga
     1381 tgcggtcctt ggcctcgtcg cgcgtccagt gccgaagcgt accggtcaat acaaacgtca
     1441 ggccggcgag cgcaccggcg ggggcacgat gcggcgcggc ttccgtccag tgcacgccgg
     1501 ccgcgcgtaa ctgctcgacc acttcgcgat tgtgcggctc ggcgaagaag cgtgcgatcg
     1561 aagcggcaac caccggtccc acatccgtga cctgcagcag ttcttcctgc gaagcatcca
     1621 tcaccgcatc gagcgacccg aaatggcgcg ccaggtcgcg cgcggtcgat tcaccgacat
     1681 ggcgaatgcc gagcgcataa atgaatcgct cgagcgtggt cgagcgcgcc cgttccagtg
     1741 cggccagcac attggcggcc gatttgtccg ccatgcgctc gagccccgcg agcgccccaa
     1801 ggcctagctt gaagagatcg gccggtgtct gcaccagccc ggcgtcgacg agttgctcaa
     1861 ccagtttgtc accaaggcct tcgatgtcga gcgcgcgccg gcccgcaaag tgcagcagcg
     1921 cctgctttcg ttgcgccgcg cagaacagac ccccggtgca acgatggtcc ttctcacctt
     1981 cctcccgggc gacgctcgat ccgcacaccg gacaggcctg gggcatcgcg aagttgcgag
     2041 cgttcttcgg acgtcgatcg ggcagcacgg atacgacctg cggaatcacg tcgccagcgc
     2101 ggcggacgat caccgtatcg ccgatcatca gtccaaggct gcggatgtag tcctcgttgt
     2161 gcagcgtcgc gttgctgacc gtgaccccgc cgacgaagac cggcgccagg cgtgccaccg
     2221 gggtgatctt cccggtccga ccgacctgca cgtcgatgcc ggtcacctgt gtcagctcct
     2281 cctgtgccgg gtacttgtgc gccacggccc agcgcggttc acgcgaaacg aatcccagcc
     2341 ggcgctgcag ttcgagcgag ttgaccttgt agacaacgcc atcgatgtcg aacggcaatc
     2401 ggtcgcggcg ctccgcgatg tcggcgtgaa acgccaccag ctcatcggca ccgctggcca
     2461 cgcgtcgctc cgcggcgacc ggcacgccca tgttcgccag cgcgtgcagc atcgcactgt
     2521 gagtgggcgg caatgtccac ccggccacct cgcccagccc ataggcgtag aaggacagtg
     2581 gccggctggc cgtcaccccg gcatcgagtt ggcgcaggaa gccggcggcg gcatttcgtg
     2641 gattcacgaa caccttgtcg ccggtctcgc gctgccgctc gttgatctgc tcgaagtcgc
     2701 gccggcgcag gaagatctcg ccgcggactt cgaggaccag cgcatcgacg ccgttcaggc
     2761 gcaaggggat cgcacggatc gtgcgcgcat tcggggtgac atcctcaccc tcctcgccgt
     2821 cgccgcgcgt cgcggcccgc accaacactc cgcgttcata gcgcaaactg acagccagtc
     2881 cgtcgaactt gagctcggcg cagtactcga tcggcggatc atccgccgcc aggcccagct
     2941 cccgccgcac gcgtgcatcg aacgcacgcg caccactggg accggcatcg gtttcagtgc
     3001 ggatcgacag catcgggacg acatggcgca ccttggggag gtcagatcgg accgcgccgc
     3061 ccactcgctg tgtcggcgat tcggacacca ccagttccgg atgcgcggct tcgagttcct
     3121 gcagttcccg gaacaagcgg tcgtactccg cgtctggaat gctcggggcg tcgagaacat
     3181 agtagtcatg atccgcccgg ccgatcaggt cgcgcagtcg ccgtgcgcgc tgacgggcgg
     3241 aatcatgccg tgccgacggc atcgatcagt gcccgatcgc ctgtctgcag ggcaccggtc
     3301 ggcccgtcga gggggaacga atccggtgag cgtggctttt catccgcgct tctttttcag
     3361 acagcgtaga gccggcgggc gcgctcggag cccgctgcaa tgccggccgc ctgcatctgc
     3421 tcgtacatcc cggccagctg cgcagcaatc gcgaaatgcg cttcccgttg caccggcttg
     3481 ccgttgtcat cgacgacgcg cgcattcaga cgagtagcga gctggtcggc aaccgcgaac
     3541 tgccgcgcca gcggagagcc ttctgggcga acgagcggaa catcgatgaa aatggccagc
     3601 cgattcgcag gcaaggcccc cgcggtcatg ctcagtgcga gttcacccga cgcctcgcgc
     3661 atttcccagc gaccggaccc cacggcggta aagccgaccg ggatcgccgc ggcctggatc
     3721 acgtcggcgg tccagggagt ctcgcccgac tccaaagtca gcgtcacttg cacgtcgaaa
     3781 cgcgcgcaaa cgcgatccag ctccgcggcc tgcgccacca gtcgcggcac gtcgggcgcg
     3841 tcgaagtccg catcaatacc gagagcgatc tgctgggtcg cggccacgaa gcgggccgca
     3901 tcgatttcgc cgagcacccc gttgcgatga gccatctgta ccgcgatcag gagctcggag
     3961 tagaagccga accgatcggg ccccgtccac tgctgcgtct tggcatccca gaccgccaga
     4021 tgcgttggca acggcaactg caggcgctca agctgaccct tcgcttcgag cgcggccaca
     4081 ccgtcgatcg catgggcgca cctcagctcg atcacgaaat ccagcagggg atcctcgctc
     4141 cagccgtcgg gaagcgcgta cgccgacggt tcgctgcgtt cgtccgcgac ctcagcgcct
     4201 tcgtcggcgc tctgatcagc cacttcgacg gccgtccgct cagcctccgg agctgtctct
     4261 tgcgaagcag ttgcagcgtc gttgccgacg ccacccgcgc catcaaggcg aggctcgatg
     4321 cgccgggatg aagccgccgg agacggcagt ggacgtgtac cgagcagcgg atcgccgaca
     4381 ccctggcgca tggccgcatc caggcgcgcc agcgcacgac gttcctgcca cttgctgtaa
     4441 atccagatcg ccagcagcag gacgccggcg atcgtaagca gcgcccagtg caggtcggtc
     4501 atcgaggtct aggccgcttc cgccgtcagg cgaacggcac cttcgatgtc aaccgccacg
     4561 atgcgggaca caccctgctc ctgcattgtt acgccgatca gctgctgcgc catttccatc
     4621 gcaatcttgt tatgcgtgat gaacaggaac tgcgtgttga cagtcatcga ctcgaccagc
     4681 cggcaaaagc gctcggtgtt cgcgtcgtcg agcggtgcgt cgacctcgtc cagcagacag
     4741 aacggcgccg gattgagctt gaacagcgct aacaccagcg cggtggccgt cagtgccttc
     4801 tcgccgcccg acaggaggtg aatcgagctg ttgcgcttgc ccggcggttg cgccatcacc
     4861 tgcacgccgg cgtcgagtat ctcctcgccg gtcatgatca gccgcgcctc gcccccgccg
     4921 aagagcttcg ggaacagctg cccgaaatgc gcattcacct ggtcgaaagt ctgctgcagc
     4981 atttcgcggg actcgcggtc gatcttgcgg atcgcgtcct cgagcgtggt gatggcggcc
     5041 ttgagatcgg ccgactgcgc atcgaggaag gacttgcgct cgcgcgatgt cgtcagctct
     5101 tccagcg
//
LOCUS       DATIRT010000160         9157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_907589, whole genome shotgun sequence.
ACCESSION   DATIRT010000160 DATIRT010000000
VERSION     DATIRT010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9157
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907589"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="VLN25_03795"
     CDS             complement(<1..1122)
                     /locus_tag="VLN25_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HSQ09726.1"
                     /translation="MRNPEREVHYFRLRVLVAVAFVIACFTLLGARFAWLQVVKHEAY
                     LAQAEMNRIAVLPVAPNRGLIKDRHGRIIARNYSAYTLEITASKVANLEQTIDQIAQV
                     VEVQPRDRRRFKKLMEDTKRFDSVPIRTRLTDEEVARFTAQRYRFPGVELRARLFRDY
                     PLGLTGSHMLGYIGRISQRDLERIEKLDDPAAYAGTEHIGKLGIEQSYEGDLHGTTGV
                     EEVEITAGGRAVRTLKRTPSMPGNNLTLSVDIELQRVVEQAFGNRRGALIAIEPRSGD
                     VLAFVSMPNFDPNLFVDGIDPKSWDELNNDPDKPLLNRALRGTYPIGSTYKPFLALAA
                     LETGKRRPETVIHDGGTFVFGNRVFRDSNKVPLGAVDMHR"
     gene            1524..1919
                     /locus_tag="VLN25_03800"
     CDS             1524..1919
                     /locus_tag="VLN25_03800"
                     /inference="COORDINATES: protein motif:HMM:NF023184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HSQ09727.1"
                     /translation="MSSTPPPPVPPLRPGRDRPTTPRLLRELRTLEVMTEIYCRDQHG
                     SETPCERCRDLLSYAAKRLAVCPYGEDKPVCAKCQIHCYGPAPREEVRTIMRYAGPRM
                     MLRHPWLALGHLLDKRYPAPAKPRARQRA"
     gene            2152..2997
                     /locus_tag="VLN25_03805"
     CDS             2152..2997
                     /locus_tag="VLN25_03805"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HSQ09728.1"
                     /translation="MAKRAVLIGVNRYRMPGADLRGCVSDVDTMTTLLTGTFGFAAGD
                     IVRLTDFAATKKAIESAIRSLVRGGRRGDVLLIHFSGHGSNVPDDDGDEADGRDEVLC
                     PADLDWKKPLRDDWLRSTFDALRTGVGLTVVTDCCHSGTITRAVVPPDAPIVERYLPC
                     PLDLLAVESGRRLRGTVRGGLHLAPTATRRKRDVVAADLPEVLITGCRADQTSADAAI
                     EGGFHGALTFNLAATLREAQGRVSYRELHERTSARLKRGRLDQVPQLEGVKARLEQPF
                     LSPVE"
     gene            3020..8758
                     /locus_tag="VLN25_03810"
     CDS             3020..8758
                     /locus_tag="VLN25_03810"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HSQ09729.1"
                     /translation="MAIELLIPGRPDAHAKGGTRGAPSEKRSTDLLDAVETVDAYTLS
                     PSARARAKAPARIAVESDDILEIEVEGGFTLWTSVERYRHDLAVLRPEAVRGNAMPID
                     MLPRNSVSERGVRDWMASALRVLRLRPDAVDAEMANPARWSEFVREAGLGHAITAGAW
                     ATTKFALWLIERRLPYGEGLYRFGDVALRSAGAADPVVLSPIDAEQPVLVFIHGAASS
                     SQGSFGALLDSSAQSDWRAIGDVFGSHIYGFEHRTMSASPIENAIRLAQALPARARLS
                     LVTHSRGGLVGDLMCLSSISTEAIACVRRVDPALAEADEVDRRNLQTLAQILAAKQFR
                     IERFVRCASPARGTLLASENTDQFLSVLTNLLGLIPGLAGSPLYEVVKRITLQVAKNR
                     WKPALIPGIEAMTPPSPLVRFLNTQHDAAGTLGVISGDIEGGHWLKQLGVFLTDQFIY
                     ENRDNDLVVNTDSMFEGATRPHAHYVFDQGGDVTHFNYFRNERTRGAIQRWLTTTGEG
                     APAEFRTIEETQTAPVPMLRALQKRAGTAQPIVFVLPGITGSQLTRADKCIWLDYTRL
                     VVDGLKELHDIAAADVRASSLVGAYYRGLCEWLADTHEVIPYAYDWRRSIVDSAARLA
                     AEVEKVLERSAGPVRFVAHGMGGLVVRQMIKDAPDLWDRVCVHEGGRLVMLGTPNHGT
                     HATVDALLGTASTVQQLAMLDLDRGLAGVLDVIQDFPGILELLPLAEGGRYFERATWD
                     HMHAQCGQGARPDPGRLARARATLAGLPEQIPHAERVLYVAGSSPQTVIGFEGVDGRI
                     VLQATSEGDGRVTYESGVLPGVQTWYLDAEHGDLPATEAGYPAILELLERGTTRRLPT
                     APPSAARGGGARFRALPQPALYPTSGDLTAGLLGQQRTKRYRARVTIGFRAGVVHGDL
                     RYARYPIVVGHYEGDTIVGAEARIDQLLEGALSTRYNLGLYPGPARTSSVVLRQPTAL
                     QRALRLPNGSVVVGLGRWGELTAAQLANLIRRAALTYVLQLDDAAGATLVGESDIVGL
                     SMLLIGTNSTSNISIDYSIAAILRGIAQANRELRSRVPAARHIEEVEIVELYADTAIE
                     AARAVRRLAAPLGEELNTRIEATPLLASGRNGRTRLTPVQGRDSWRRWEISVIDPPAA
                     GAVPRLPKPLAERLQAAISQAATTDGELVAALAQLALCEAPAHTPHRELKFVSLSDRA
                     RAEVMHQQRQPELVERLIAASVAQTRFRAEEARALFELLVPNDLKDGLAQLSRVVFVV
                     DAETAAYPWELMTDGKEPLATRMGLIRQLKTATFRQHIRATTAQTAYIVGDPLVSAPY
                     QQLSGARDEARLVAGLLSTRFEPTYRDEQLSALEVLGGLFEKPYRLIHLAGHGYYEAP
                     GDNGAARSGMVLDNGVYLTAVEIGQMQQVPELVFLNCCHIGQIGPEAPARGAALPYNR
                     LAASISRELIEMGVRAVVAAGWAVRDDAALAFARVFYEQMLEGQSFGRALQEARHRTW
                     QQFPDCNTWGAYQAYGDPDFRLDQDAAASSRSTSMRDYVSTAEFIDVLRSSRQTAREI
                     ERGERKGDASQFRPAAARRIADLVKDCPSEWLNRSEVLVELGLAFGELADFEQAARYL
                     SEALASDELDSCTTLAAVERLANFESRLGERLATAVGADAATRARGAASIETAISRLI
                     TLGQLGQTAERLALLAGCYKRLAQIRSDRDEIRLALEQSARHYQKAHEYNSERSRFDP
                     YPVLNWLALDTVLGVSQPELDTLLARVEGAARERYARSKDFFDAVALAEANLVRALGT
                     GALFGDASRVAAEVERVASLYTQAIRVAQATARQVDSATNQITTLARMLRALSVRDAA
                     AEQTAAALLALRSRISGEATGAAADQAPTAPAPDRSPRKPVPPATSQRRKAAATAVPP
                     KRRRRSSSGISG"
     gene            8872..>9157
                     /locus_tag="VLN25_03815"
     CDS             8872..>9157
                     /locus_tag="VLN25_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09730.1"
                     /translation="MRSPVLVSTLMVFVLAIAGCADLLPKSKVQTEVQWTSFNEARAV
                     IDGIKPYATTKADLASIGVTEVNPAVTLLSHAEVAIRFPIGGVLNEQDVDP"
BASE COUNT         1506 a         3120 c         3110 g         1421 t
ORIGIN      
        1 gcggtgcatg tcgaccgccc ccaacggcac cttgttcgag tcgcggaaca cgcggttgcc
       61 gaagacgaag gtgccgccgt cgtggatgac cgtttcgggc cggcgcttgc cggtctcgag
      121 cgccgccagc gcgaggaacg gcttgtaggt cgaaccgatc ggataggtgc cccgcagcgc
      181 gcggttgagc aggggcttgt ccgggtcgtt gttcagctcg tcccagcttt tcgggtcgat
      241 gccgtcgaca aagagattgg ggtcgaagtt cggcatcgag acgaaggcaa gcacatcgcc
      301 ggacctgggt tcgattgcga tcagcgcgcc gcggcggttg ccgaaggcct gctcgaccac
      361 acgctgcagc tcgatatcga ccgatagcgt caggttgttg ccgggcatcg acggcgtgcg
      421 cttcagggtg cgcaccgccc ggccgcccgc ggtgatctcg acttcctcga ccccggtggt
      481 gccgtgcagg tcgccttcat agctttgctc gatgccgagc ttgccgatgt gctccgtacc
      541 tgcgtacgcg gcgggatcgt cgagcttctc gatgcgctcg aggtcgcgct gggaaatgcg
      601 gccgatatag cccagcatgt gcgaccccgt caggccgagc gggtagtcgc ggaacaggcg
      661 cgcacgcagc tcgaccccgg ggaagcggta acgctgtgcg gtgaagcgcg cgacctcctc
      721 gtcggtcagg cgggtgcgga tcggcaccga atcgaagcgc ttggtgtcct ccatcagctt
      781 cttgaaccgg cgccggtcac gcggctggac ttcaaccacc tgggcgatct ggtcgatggt
      841 ctgctcgagg ttcgcgactt ttgacgcggt gatctcgagc gtgtaggcgg agtagttgcg
      901 cgcgatgatg cggccgtggc ggtccttgat cagtccgcgg ttcggggcga ccggcagcac
      961 ggcgatgcgg ttcatctcgg cctgcgcaag gtaggcctcg tgcttcacga cctgcagcca
     1021 cgcaaagcgt gcgcccagca gcgtgaagca ggcaatgacg aacgcgacgg ccaccagcac
     1081 gcgcaggcgg aagtagtgca cttcgcgctc gggattgcgc attgcggccg agcgcggacc
     1141 gaacgacaac tcgtcaccgc ttgagcgccg acggcggcgc agccagcgtc tacccgacat
     1201 gacggtacgc gacgacgatg ctgctgacag cgtgcacggc acgaggaagc ggcagacgcc
     1261 gctggggaca gatggcaacc actcggagga tcagcgccgg agcgcagaca gtaaacgggg
     1321 cggattatag gagcgcccct caagtgacat gccgcgaaaa ccgcggcaaa cgtcacccgg
     1381 caccccggct cacctcggcg ggtgccccgc aacgtcgctt ggggacccca aggcggcagg
     1441 gatggccccg tcctgcgatc ccgcaccgca tcggtttgat ccggctcagc acgcgatcgc
     1501 ggcgtgccgg tcgacaatgc ggcatgtcca gcacccctcc cccgcccgtc ccacccctgc
     1561 ggcccggtcg cgaccggccg accaccccgc gcctgctgcg cgaactgcgc acgctggagg
     1621 tgatgacgga gatctactgc cgtgaccagc atggcagcga aacgccgtgc gaacgctgtc
     1681 gcgacctgct gagctacgcg gccaaacggc ttgcggtgtg cccctacggc gaggacaagc
     1741 cggtctgcgc caagtgccag atccactgct acggaccggc gccgcgcgaa gaggtacgaa
     1801 ccatcatgcg ttacgccgga ccgcgcatga tgctgcggca cccgtggctg gcgctgggtc
     1861 acctgctcga caaacgctac cccgctcctg ccaagccgcg cgctcgccag cgcgcctgaa
     1921 actctcctgg attcaccgcc tgacctgcgc acatcgcgca gcggtccgcc gtcccccata
     1981 cgtggcatgg cggccgcggg tgctgtcgaa tcttcgggcg cgccggcgtg cccggaggac
     2041 tagcatcgcg gcggggatca cggcggaccc atagtcagcc gtgaggtggg tgggcggtaa
     2101 gcccggagcg cgagacgcag ttcgcccgcg gccggtggaa agggggatcg aatggccaag
     2161 cgcgcagtgc tgatcggagt caaccgttac cgcatgcccg gagccgacct gcgcggctgc
     2221 gtcagcgatg tcgacacgat gacgacgctg ctgaccggca cattcggctt cgctgccggc
     2281 gacatcgtgc gactgaccga cttcgccgcg accaaaaagg ccatcgagtc tgcgatccgg
     2341 tcgctggtgc ggggcggccg gcgcggcgac gtgttgctga tccacttctc cgggcatgga
     2401 tcgaacgtgc cggacgacga tggcgacgag gccgatggcc gcgacgaagt attgtgtccg
     2461 gcggatctcg actggaagaa gcccctgcgt gacgactggc tgcgttcgac cttcgatgcg
     2521 ttgcgcaccg gcgtcggcct gaccgtggtc accgactgtt gtcactccgg cacgatcacg
     2581 cgggccgtcg tgccgcccga tgcaccgatc gtcgagcgct acctgccctg tccgctggac
     2641 ctgctggcgg tcgaatccgg gcgacggctg cggggcacgg tgcgcggcgg cctgcatctc
     2701 gcgcccactg cgacgcggcg caagcgcgac gtcgtggcgg ccgatctgcc cgaggtcctg
     2761 atcaccggtt gccgcgccga ccagacatcg gccgatgctg cgatcgaggg aggtttccac
     2821 ggcgcgctga ccttcaacct tgcggccacc ctgcgcgagg cccaggggcg cgtcagctac
     2881 cgtgaactgc acgagcgcac gagcgcgcgc ctgaaacgcg gccgtttaga tcaggtcccg
     2941 cagctcgaag gggtgaaggc acggctcgag cagccgttcc tgtcgcccgt cgaataggga
     3001 aaccgcaggc tacgaatcca tggcgatcga actcctgata cccggccggc ccgacgcgca
     3061 cgcgaaggga ggcacgcgcg gcgcgccgtc cgagaaacgc tcgaccgacc tgctcgatgc
     3121 cgtggaaacg gttgatgcct acacgctgtc gccgtcggca cgtgcgcgcg cgaaggcccc
     3181 cgcgaggatc gccgtcgaaa gtgacgacat cctcgagatc gaagtcgagg gcggtttcac
     3241 gctctggacg tcggtcgagc gctaccgcca cgacctggca gtgctgcgcc cggaggcggt
     3301 gcgcggcaac gcgatgccga tcgacatgct gccgcgcaac agcgtgtccg aacgcggtgt
     3361 gcgcgactgg atggcgagcg cactgcgggt gttgcggctg cggcccgatg cggtcgatgc
     3421 cgaaatggcc aatcccgcac gctggagcga gttcgtgcgc gaagccgggc tcggccacgc
     3481 gatcacggcc ggtgcctggg ccacgaccaa gtttgcgctc tggctgatcg agcggcgcct
     3541 gccgtacggc gaaggcctgt accgcttcgg cgacgtggcg ctgcgttccg ccggcgcggc
     3601 agatccggtc gtgctctcac ccatcgatgc cgagcaaccg gtcctcgtgt tcatccatgg
     3661 cgcggcctcg tcctcacagg gcagcttcgg cgcgctcctc gactcgtcgg cccagtccga
     3721 ctggcgagcg atcggcgacg tgtttggcag ccacatctac ggcttcgagc accggacgat
     3781 gagcgcaagc ccgatcgaga atgcgatccg gctggcacaa gcgctgccgg cacgcgcgcg
     3841 cctgtcgctg gtcacgcatt cgcgcggcgg cctggtcggc gacctcatgt gcctgtcgtc
     3901 cattagtacg gaggccattg cgtgcgtccg gcgcgtcgac cctgcgctcg cagaagccga
     3961 cgaagtcgac cggcgcaacc tgcagacgct cgcacagatc ctggcagcaa agcagttccg
     4021 catcgaacgc ttcgttcgct gcgcgagccc cgcacgcggc acgctgctcg cctcggaaaa
     4081 caccgaccag ttcctgtcgg tgctgaccaa cctgctgggt ctgattccgg gcctggccgg
     4141 ctcgccgctg tacgaggtgg tcaagcgcat cacgctgcag gtcgcgaaga atcgatggaa
     4201 gccggcactc attccgggca tcgaggcaat gacgccgccc tcgccgttgg tgcgtttcct
     4261 gaacacgcaa cacgacgccg cggggacgct cggtgtgatc tccggcgaca tcgagggtgg
     4321 tcactggttg aagcagcttg gcgtattcct taccgaccag ttcatttacg agaaccgcga
     4381 caacgatctc gtcgtcaaca cagactcgat gttcgaaggc gcgacgcggc cgcacgcgca
     4441 ctatgtcttc gaccagggcg gcgacgtcac tcacttcaac tacttccgca atgaacgcac
     4501 gcgcggcgcg atccagcgct ggctgaccac gacgggcgag ggggctccgg cggagttccg
     4561 gacgatcgaa gagacccaga ccgcgccggt gccgatgctg cgcgccctgc agaagcgcgc
     4621 cggcacggcg caaccgatcg tctttgtgct gcccggcatc accggctcgc aactgacacg
     4681 cgccgacaaa tgcatctggc tggactacac gcggctggtg gtcgacgggc ttaaagagct
     4741 gcacgatatc gcggccgccg acgtgcgtgc ctcgtcgctg gtcggggcgt actaccgcgg
     4801 actgtgcgag tggctcgccg acacgcacga ggtcattccg tacgcctatg actggcgccg
     4861 ctcgatcgtc gacagtgcgg cccggctggc ggcggaggtc gagaaggtgc tggagcgcag
     4921 cgccgggccg gtgcggtttg tcgcgcatgg catgggcgga ctggtggtcc gccagatgat
     4981 caaggacgcg cctgatctgt gggatcgcgt gtgcgtgcac gagggtggtc gcctggtgat
     5041 gctcggcacg ccgaaccacg gcacgcacgc gaccgtggat gcgctgctcg gcaccgccag
     5101 cacggtgcag cagctggcga tgctggatct cgaccgcggg ctggccggag tgctcgacgt
     5161 catccaggac ttcccgggaa tcctggagct gctgccgctg gccgaaggcg gtcgttactt
     5221 cgagcgcgca acctgggacc atatgcacgc ccagtgcggg cagggcgcac gccccgaccc
     5281 cggtcgcttg gcaagggcac gagccactct cgccggcctg cccgaacaga tcccgcatgc
     5341 cgagcgggtg ctgtacgtcg ccggaagttc gccgcagaca gtgatcggct tcgaaggagt
     5401 cgatgggcgc atcgtcctgc aggcaaccag tgaaggcgac ggtcgcgtga cctacgagtc
     5461 cggcgttctg ccgggagtgc agacgtggta cctggatgcc gagcacggcg acctgccggc
     5521 cacggaggcc ggttaccccg cgatccttga actgctcgag cgcggcacga cccgccgcct
     5581 gccgacggcg ccgcccagcg cggcccgcgg gggcggcgcc cgcttccgcg cactgccgca
     5641 acccgcgctg tacccgacgt ccggcgacct gaccgccggc ctgctggggc agcagcggac
     5701 caagcgctac cgcgcccggg tgaccatcgg ctttcgcgcc ggtgttgttc acggcgatct
     5761 gcgctacgcc aggtatccga tcgtcgtcgg tcactacgag ggcgatacga ttgtcggagc
     5821 cgaagcccgc atcgatcagt tgctcgaagg cgcgctcagc acacgctaca acctcggcct
     5881 gtatccgggg ccggcccgga catcgtccgt ggtgctgcga cagcccaccg ccctgcagcg
     5941 agcacttcgg ttgcccaatg gttcggtcgt ggtcggactc ggccgctggg gagaactgac
     6001 cgcagcgcag ctcgccaacc tcatccgccg cgcggcactg acctacgtgc tgcagctcga
     6061 cgacgctgcc ggcgcgacgc tggtgggtga aagcgacatc gtcgggctga gcatgctgct
     6121 gatcgggacc aactcgacgt cgaacatctc gatcgactat tcgatcgcgg cgatcctgcg
     6181 cggcatcgcg caggcgaacc gcgaactgcg gagccgcgtg ccggccgcgc gccacatcga
     6241 ggaagtcgag atcgtcgagc tctacgccga caccgcgatc gaggcggcgc gtgcagtccg
     6301 gcggctggca gctccgctcg gcgaggagct caacacccgc atcgaggcca cgccactgct
     6361 tgccagcgga cgcaacggac gcacgcgcct gaccccggtg caggggcgtg actcgtggcg
     6421 acgctgggag atcagcgtga tcgatcctcc cgccgccggg gcagtcccgc gcctgccgaa
     6481 gccgctggcg gagcgtcttc aggccgccat cagccaggcc gctaccaccg atggcgaact
     6541 ggtcgccgcc ctggctcaac tcgcgttatg cgaggccccg gcgcacacac cgcatcgcga
     6601 gctgaagttc gtttcgctgt ccgatcgcgc gcgcgccgag gtcatgcatc agcagcggca
     6661 acccgaactg gtcgagcggc tgattgccgc atcggtcgcg cagacgcgct ttcgcgccga
     6721 ggaggcgcgg gcgctgttcg agctgcttgt gccgaacgat ctcaaggacg gactggcgca
     6781 actgtcgcgc gtggtgttcg tcgtcgatgc cgagacggcc gcgtatccgt gggagctgat
     6841 gaccgacggc aaggagccgc tggccacgcg catgggtctg atacgccagt tgaagacggc
     6901 cacgttccgc cagcacatcc gcgcgacgac ggcccagacg gcctacatcg tcggcgatcc
     6961 cctggtcagt gcaccctacc agcagctgag cggtgcacgc gacgaagcgc ggctcgtcgc
     7021 cgggctgcta tcgacacgct tcgagccaac ctatcgcgac gaacagctgt ctgcactcga
     7081 agtcctgggc gggctcttcg agaagcccta ccggctgatc cacctcgcgg gccacggcta
     7141 ctacgaagcc cccggcgaca acggcgccgc acgcagcggc atggtgctcg acaacggcgt
     7201 gtatctgacg gctgtcgaga tcggccagat gcagcaggtt ccggagctgg tgttcctcaa
     7261 ctgctgccac atcggccaga tcgggcccga ggctccggcg cgtggtgccg ccctgcccta
     7321 caaccggctg gcggccagca tttcacgcga actgatcgag atgggcgtgc gcgcggtggt
     7381 cgcggccggc tgggcggtgc gtgacgacgc cgcactcgcc ttcgcgcgtg tgttctacga
     7441 gcagatgctc gaaggccagt cgtttggtcg cgcgctgcag gaggcccgcc atcgcacctg
     7501 gcagcagttt cccgactgca acacgtgggg tgcttaccag gcctacggcg atcccgactt
     7561 ccgcctcgac caggacgctg cggcaagcag ccgcagcacg agcatgcgtg attacgtgtc
     7621 gacggcggag ttcatcgacg tgttgcgtag cagccgccag actgcgcgcg aaatcgaacg
     7681 tggcgagcgc aagggcgatg cgagccagtt ccgtcccgct gcggcccggc ggatcgcgga
     7741 cctggtcaag gactgtccga gtgagtggct gaaccgcagc gaggtgctgg tcgaactcgg
     7801 gctggcgttc ggcgaactgg ctgacttcga gcaggccgcg cgttacttgt ctgaggcgct
     7861 cgcctcggac gaactcgaca gctgcacgac gctggcggcg gtcgagcggc tggcgaactt
     7921 cgaatcccgg ctcggtgaac gactcgcgac ggccgtcggc gccgatgcag caacgcgtgc
     7981 gcgcggcgcg gccagcatcg aaaccgcaat cagccgcctg atcaccctcg gccagctcgg
     8041 gcagacggcc gaacggctgg cgctgctcgc cggctgctac aagcggcttg cacagatccg
     8101 ctccgatcgc gacgaaatcc gcctcgcgct cgagcagagc gcgcgccact atcagaaggc
     8161 gcatgaatac aacagcgagc gcagccgctt cgatccttac cccgtcctca actggctggc
     8221 actcgacacg gtcctcggcg tcagccagcc ggaactcgac acgctgctcg cgcgcgtcga
     8281 aggggctgca cgcgaacgct atgcgcgcag caaggacttc ttcgatgcgg tcgcgctcgc
     8341 cgaggcgaac ctcgtgcgcg cgctcggcac cggcgcgctg tttggcgacg cctcgcgtgt
     8401 ggctgccgag gtcgaacgcg tcgcgtcgct ctacacgcag gcgattcgcg tcgcacaggc
     8461 gaccgctcgg caggtcgact cggcgaccaa ccagatcacc acgctggccc gcatgctgcg
     8521 cgcactgtcg gtgcgcgatg cggcagcgga gcaaacggct gccgccctgc ttgcgctgcg
     8581 ttcgcggatc tccggggaag cgaccggtgc agccgcggat caagccccga ccgcaccagc
     8641 accggatcgc tcgccccgca aacccgtgcc gccggcgacg tcacagcgtc gcaaggcggc
     8701 cgcaacagcg gtcccgccaa agcgccgccg caggagcagc agcggaatct ctggctaggc
     8761 gtgccgtccg tcatcctggc ccccggcttt cgaatcggcg tttgacccga cacaaccggc
     8821 cgcgctagat cgcgtcgatg atgaaatcag gagtgaggtc aggaccggac gatgcggtcc
     8881 cctgtactgg tgtccacgct gatggtgttc gtcctcgcaa tcgcggggtg cgccgacttg
     8941 ctgccgaagt cgaaggtgca gaccgaagtg cagtggacga gcttcaacga agcgcgcgca
     9001 gtcatcgacg gcatcaagcc ctatgccacc accaaggcgg atcttgcctc catcggcgtc
     9061 acggaagtca atccggccgt cacactgctc tcgcatgccg aggtggcgat ccgctttccg
     9121 atcggcggtg tactcaatga gcaggatgtc gaccccg
//
LOCUS       DATIRT010000161         2738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1399531, whole genome shotgun sequence.
ACCESSION   DATIRT010000161 DATIRT010000000
VERSION     DATIRT010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399531"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1506
                     /locus_tag="VLN25_03820"
     CDS             <1..1506
                     /locus_tag="VLN25_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HSQ09731.1"
                     /translation="TYKAVARMLDEKRAERESFIDSALARLRAMVADAGLRAEISGRP
                     KHIYSIWNKMRAKGLAFEQVYDAQCYQVLALVHSEWNPVESEYDDYIARPKPNGYQSL
                     HTVVGDDAGRTLEVQIRTRRMHEHAELGVAAHWRYKEGGKGRDDDELRVAWLRQLLAW
                     RSEVDAPLATGPAREERIYVLTPQARVIELPAGGTPIDFAYHIHSELGHRCRGARVNG
                     AMVPLSTTLATGQTVEIVSAKTGGPSRDWLNPELGYLHSARSRAKVRQWFNALEFEQS
                     VAAGREIVEKELQRLGKTAVRLEELARRLGFDKVDELCAAATKEEFSLRSIEQAIAPA
                     QPAEELPPPLLTGKPREAPAGKGKVLVVGVDSLLTQLARCCRPAPPDEIGGYVTRGQG
                     VSIHRANCSNLQALTQRQPERIVEVAWGRQADAVYPVELLVIAQDRQGLLRDISEVFA
                     REKLNVVGVGTQSQRGEARMGFTVEVRDAGDIARVSALLREVSGVIAVRRR"
     gene            complement(1521..2330)
                     /locus_tag="VLN25_03825"
     CDS             complement(1521..2330)
                     /locus_tag="VLN25_03825"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HSQ09732.1"
                     /translation="MARSNDTTRAGAQAAGPAEPRLAVSLTHSPADIEDAQRLRYKVF
                     VEEMGAKVGDAVAGIDADDFDPYCDHLIVRDLDTLRVVGTYRILAPHRARELGRLYSE
                     GEFDLSRLHHIRPTMVEVGRSCVHRDYRSGSTILQLWAGMAHYMRAGGYNHLIGCASI
                     PLADGGRMAARARLDLQKFLTDPEYRVFPHLPFPYAQIEPATTGEMPPLLKGYLRVGA
                     KICGEPAWDPDFNTADFLVWLSLHNLRPRYARHFELLAAQGNAAERASSNF"
     gene            2513..2589
                     /locus_tag="VLN25_03830"
     tRNA            2513..2589
                     /locus_tag="VLN25_03830"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2547..2549,aa:Val,seq:gac)
BASE COUNT          498 a          846 c          943 g          451 t
ORIGIN      
        1 acctacaagg cggttgcgcg catgctcgac gagaagcgcg ccgagcggga gtcgttcatc
       61 gattcagctc tggctcgcct gcgcgcgatg gtggccgacg ccgggctgcg cgcggagatc
      121 agcgggcgac ccaagcatat ctacagcatc tggaacaaga tgcgcgccaa gggactcgct
      181 ttcgagcagg tctatgacgc gcagtgctat caggtcctgg cgctcgtaca ctcggagtgg
      241 aacccggtcg agagtgaata cgacgactac atcgcccgac ccaagcccaa tggataccag
      301 tcgctccaca cggtcgtcgg cgatgacgcc ggccgcacgc tcgaagtcca gatccggacc
      361 cggcgcatgc atgagcatgc cgagcttggc gtggctgccc actggcgcta caaggaaggc
      421 ggtaaggggc gcgacgacga tgaattgcgg gtggcgtggc tgcgccagct gctcgcctgg
      481 cggtccgagg tcgatgctcc tctcgccacc gggccggcac gcgaggagcg catctacgtc
      541 ctgacgccgc aggcgcgcgt gatcgagttg ccggccggtg gcacgccgat cgacttcgcg
      601 tatcacatcc actccgagct gggacaccgc tgccgcggtg cacgcgtcaa cggggccatg
      661 gtgccgctga gtacgacgct cgcgacgggg cagacggtgg aaatcgtgtc ggccaagacc
      721 ggcgggccga gtcgcgactg gctcaacccg gaactcggtt acctgcacag cgcacgctcg
      781 cgcgccaagg tgcgccagtg gttcaacgca ctcgagttcg agcagtcggt ggcggccgga
      841 cgcgagatcg tcgagaagga actgcaacgg ctgggcaaga cggctgtcag gctcgaagag
      901 ctggcaaggc gattgggatt cgacaaggtc gacgagctgt gcgctgcggc gacgaaggaa
      961 gagttcagcc tgcgttccat cgagcaggcg atcgcaccgg ctcagccggc agaggaactc
     1021 ccgccgcctc tgctgaccgg caagccacgc gaagccccgg ccggcaaggg caaggtgctg
     1081 gttgtcgggg tggattctct gctgacccag cttgctcgct gctgccggcc cgccccaccg
     1141 gacgagattg gcggatatgt gacgcggggc cagggagtat cgatacaccg cgcgaactgt
     1201 tcgaatctgc aggccctgac ccaacgccag cccgagcgca tcgtcgaggt ggcctggggc
     1261 cggcaggccg atgcggtcta tccggtcgag ctgctggtca tcgcgcagga ccgccagggg
     1321 ctgctgcgcg acatctcgga ggtcttcgcg cgcgaaaagc tcaacgtcgt cggcgtcggc
     1381 acgcagagcc aacgcggcga ggcgcgcatg ggattcacgg tcgaagtacg tgacgcgggt
     1441 gacatcgcac gggtgtctgc gctcctgcgc gaggtatccg gcgtgatcgc ggtacgccgt
     1501 cgctgaggcg gtggccggcg tcagaaatta gacgaggccc tttcggcggc attgccttgc
     1561 gccgcgagca gctcgaagtg acgcgcatag cgcggacgga ggttgtggag cgaaagccac
     1621 accaggaagt cggctgtatt gaagtcgggg tcccacgcag gctcgccaca gatcttcgcg
     1681 ccgacacgca gatagccttt caacaagggc ggcatctctc ccgtggtcgc aggctcgatc
     1741 tgcgcgtacg gaaagggcag gtggggaaac acgcggtact cgggatcggt caggaacttc
     1801 tgcagatcca gcctcgcgcg cgcggccatg cgcccgccgt cggccagcgg aatgctggcg
     1861 cagccgatca ggtggttgta accgccggcc cgcatgtagt gggccatgcc agcccacagc
     1921 tgcaaaatgg tgctgccgga gcgatagtcg cgatgcacac aggaacggcc cacctcgacc
     1981 atcgtcggcc tgatgtgatg caggcgtgac aggtcgaact cgccttccga gtagagccgt
     2041 cccagctccc gggctcggtg cggcgccagg atgcgatacg tgccgacgac ccgcagcgtg
     2101 tcgaggtcgc ggacaatcag atggtcgcag taggggtcga agtcgtccgc gtcgatgcct
     2161 gcgacagcgt cgcccacctt cgcgcccatc tcctcgacga acaccttgta gcgcagtcgc
     2221 tgggcatcct cgatgtcggc gggcgagtgg gtcagggata ccgcaagccg cggctcggcc
     2281 ggaccggctg cctgggctcc tgcgcgtgtt gtgtcgttcg acctggccat gggaccctga
     2341 gcaaatgtga ctgcggggcc aagactaaat tgcatcgatg acgctaaagt gaacggactg
     2401 agcgccgtta ctcgacgtca tgttcggcga ggggcggtgg cgctgtcaat ggacgccccg
     2461 cggcccgtat ggccgccggc tcgggatgcg ctgatatact tcgaagcctt ggaggcgcgt
     2521 agctcagctg gttagagcac caccttgaca tggtgggggt cgttggttcg agtccaatcg
     2581 cgcctaccag gccccaaagc agacgaaagg tacggagaaa tgcttcgaac tcgattcacg
     2641 gacggttgat ccccggagac gagtacgcct ttcgacggaa aagtgcggct cgaccgcact
     2701 tttttttcgc ttggagattt ccatggtctc agttcgat
//
LOCUS       DATIRT010000162         2251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1694563, whole genome shotgun sequence.
ACCESSION   DATIRT010000162 DATIRT010000000
VERSION     DATIRT010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694563"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /gene="ccoG"
                     /locus_tag="VLN25_03835"
     CDS             <1..1041
                     /gene="ccoG"
                     /locus_tag="VLN25_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HSQ09733.1"
                     /translation="EQKIEGDRARRMKLDKEPMSARKFALKAAKHGAWIAVALWTGFT
                     FVGYFTPIRGLTIDVATLAAGGWSLFWILFYGFATYGNAGWLREQVCKYMCPYARFQS
                     AMFDPDTLIVTYDRERGEPRGAGSKNSTRAEQNLGDCVACKLCVQVCPTGIDIRDGLQ
                     YECIGCAACIDACDFVMERAGMPKGLIRFSTERALKEHYAARGIWGHVLRPRVFVYTG
                     ILAAVTAALVVAVGIRVPLKVDVIRDRGALAREIEDGRIENTYRLQVMNTSESGQVFA
                     LSAGGLPGLQIASEPTFEMPGASTRSVLVRLRARPEDIAPGSNKVVIGVKSVDDPKVA
                     VEEKTVFLGLRR"
     gene            1068..1610
                     /locus_tag="VLN25_03840"
     CDS             1068..1610
                     /locus_tag="VLN25_03840"
                     /inference="COORDINATES: protein motif:HMM:NF017559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HSQ09734.1"
                     /translation="MNTQAVTPWYRQPWPWFLIALPATAVIASIATAIIAARGFDGLV
                     AADYYKQGLAINDEVARAQLARDLGLEARIELAGITDGDRVRVEIDAQRALPPEAALR
                     LRLVHPGRPGEDRTAVLSRQDVDPENRRALYVGTLQAVAPTSSYVGPVSWQVILESQQ
                     WRVDDSFTAGGGGRFTLQAR"
     gene            1646..1978
                     /locus_tag="VLN25_03845"
     CDS             1646..1978
                     /locus_tag="VLN25_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09735.1"
                     /translation="MTDSFAQRLGWVLWPSFLVACAAELAFFSLFDPRDLHLFGAPVE
                     ADRMLVYTLGFFALWALGAISSALTVFLARSPFEVNRCTLPAEDRPDGCPKREGVSDV
                     AVVPLERS"
     gene            complement(2016..>2251)
                     /locus_tag="VLN25_03850"
     CDS             complement(2016..>2251)
                     /locus_tag="VLN25_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003268377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSQ09736.1"
                     /translation="AFLLNLSQRFEARGYSRTEFVLRMTRAEIGSVLGLKLETVSRVL
                     SRFAQDGLIDVNQKHVRIVNQDGLRQIISGQGS"
BASE COUNT          367 a          692 c          789 g          403 t
ORIGIN      
        1 gaacagaaga tcgagggcga tcgcgcccga cgcatgaagc tcgacaagga gccgatgtca
       61 gcgcgcaagt tcgcgctcaa ggccgccaag cacggagcgt ggatcgcggt ggcgctctgg
      121 accggcttca catttgtcgg ctacttcacg cccattcgcg gactgacgat cgatgtcgcc
      181 acgctcgctg ccggcggctg gtccctgttc tggatactgt tctacggctt cgcgacctac
      241 ggcaatgcgg ggtggctgcg cgagcaggtc tgcaagtaca tgtgtcccta cgcgcgcttc
      301 cagagcgcaa tgttcgatcc ggacacgcta atcgtcacct acgaccgcga gcgcggcgag
      361 ccgcgcggtg caggcagcaa gaactcgacg cgcgccgagc agaacctcgg cgactgcgtg
      421 gcgtgcaagc tgtgtgtcca ggtctgcccg accggcatcg acatccgcga cggcctgcag
      481 tacgagtgca tcggctgcgc cgcgtgcatc gacgcctgcg acttcgtgat ggaacgggcc
      541 gggatgccga aggggctgat ccgcttttcg accgagcgag ccctgaagga acactacgca
      601 gcgcgcggca tctggggaca cgtactccgg ccgcgcgttt tcgtctacac cggcatactc
      661 gcggcggtca cggcagcgct ggtggtggcg gtcggcatcc gcgtgccgct gaaggtggat
      721 gtgattcgcg atcgcggtgc gctggcgcgt gagattgaag acggacgcat cgagaacacg
      781 tatcggctgc aggtgatgaa caccagcgag agcggtcagg tctttgcgct cagtgccggt
      841 ggcctgcccg gactgcagat cgcctcggag ccgacgttcg aaatgcctgg cgcgtcgacg
      901 cgctcggtcc tggtgcggct gcgggcgcgc ccggaggaca ttgctccggg ctcgaacaag
      961 gtcgtgattg gcgtgaagtc ggttgacgat ccgaaggtgg cggtcgaaga gaaaaccgtc
     1021 tttctcgggc tgcggcgttg agcatgccca caaccaagga gcaaattgtg aacacgcagg
     1081 ccgtgacccc gtggtaccgc cagccgtggc cctggttcct catcgcactg ccggcgaccg
     1141 cggtcattgc aagcattgcc accgccatca ttgctgcccg aggcttcgac ggactggtgg
     1201 cggccgacta ctacaagcag ggtctggcga tcaatgacga agtggcccgc gcgcagcttg
     1261 cgcgcgacct cggcctcgag gcgcgcatcg agcttgccgg cattaccgac ggtgatcgtg
     1321 tgcgtgttga gatcgacgct cagcgggccc tgccgccgga ggcggcgctg cggctgcggc
     1381 tggtgcatcc tggccggccg ggtgaggacc gcacggccgt gctgtctcgc caggacgtcg
     1441 atccggaaaa tcgccgcgcg ctgtacgttg ggacgctgca ggctgtggcg cctacgtcga
     1501 gttatgtcgg acccgtcagc tggcaggtca ttctcgaatc gcagcaatgg cgcgtcgatg
     1561 attcgttcac cgccggcggc ggcggtcgct tcacgctgca ggcgcgttag tccttcagaa
     1621 gaccgcggaa cggcgaggag gaggcatgac agacagtttt gcgcaacggc tcggctgggt
     1681 gctgtggccg tcgttcctgg tggcctgcgc ggccgaactg gcgttctttt cgctgttcga
     1741 tccgcgcgac ctgcatctgt tcggagcccc ggtggaggcc gaccggatgc tggtctacac
     1801 actgggattc ttcgcgctgt gggcgctcgg cgcgatatcg agtgcgctga ccgtcttcct
     1861 cgcgcgctcg cctttcgagg tcaaccgctg cacgttgccg gcggaggatc gaccggacgg
     1921 ctgcccaaag cgggaaggcg tatcagatgt cgcggtggta ccgctcgagc gcagctagca
     1981 gctcagccct gcgcggcggg tgatgcagcc ggtggctagc tgccctggcc ggaaatgatt
     2041 tgccgcaaac cgtcctgatt gacgatgcgg acgtgcttct ggttgacgtc gatgaggccg
     2101 tcctgcgcga agcgggacag cacgcggctg acggtttcga gtttgaggcc gaggacgctg
     2161 ccgatctccg cgcgggtcat gcgcagcacg aactcggtgc gcgagtagcc gcgcgcttcg
     2221 aaacgctgcg acaggttcag gaggaaagcg g
//
LOCUS       DATIRT010000163         5252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_907660, whole genome shotgun sequence.
ACCESSION   DATIRT010000163 DATIRT010000000
VERSION     DATIRT010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5252
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907660"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1412)
                     /locus_tag="VLN25_03855"
     CDS             complement(<1..1412)
                     /locus_tag="VLN25_03855"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HSQ09737.1"
                     /translation="MGRRERMSSVDTAWLRMDSQANLMMIVGVYEFVGNVDYDRMRRL
                     LEERLLSHARFRSCVVQDATGYYWELDDNFDLDLHLARTALPGKGTTADLKRLVGRLA
                     STALDSTKPLWQMHLVDNYNGGQAMIVRIHHCIADGIALIGVVMALTSEDPEDGGVEE
                     PGRRSRAASGGSWDAWLRPLTSATVRAIDSTGGAAVRALKASAALLAHPDSVSGAAGD
                     MARLAAQVTKDFAGIALMDNDSVTSLKGLPSGSKVVAWNEPLALDDVKAVGKALGCSV
                     NDVLLSCVSGAIRSYLEHQGEEVDGIELRAMVPVNLRDPRTWKELGNKFGLVPLLLPV
                     GIANPISRVYEVRRRMDELKQSYTAVLSMGILGVVGFAPRGLQKQALDFLAKKATAVM
                     TNVPGPQQPLYMAGTEVKRMMVWVPQSGNIGVGVSILSYAGGVQFGLITDKRLCEEPQ
                     RIIDRFEPEFEKLVLTLCMLP"
     gene            1570..1827
                     /locus_tag="VLN25_03860"
     CDS             1570..1827
                     /locus_tag="VLN25_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HSQ09738.1"
                     /translation="MSDLKSKFEKAVADSKNLPERPDNQTLLKLYALFKQASTGDVEG
                     KRPGFTDMVGRAKWDAWNEIKGTATDDAMKQYVALIEDLKV"
     gene            complement(1944..2342)
                     /locus_tag="VLN25_03865"
     CDS             complement(1944..2342)
                     /locus_tag="VLN25_03865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HSQ09739.1"
                     /translation="MADIRMIKYHSLPMAQARTLVQQAADDLAEEYDLTSQWAGNTLR
                     FRRSGVQGEMRVTESEIDLEVTLGFLLKAFKAKFVEHIEHNFDRLLAKAHAAHKPAQK
                     PGTASTKAPAKEAARKKPAVKPAKKATRKG"
     gene            complement(2357..3343)
                     /locus_tag="VLN25_03870"
     CDS             complement(2357..3343)
                     /locus_tag="VLN25_03870"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HSQ09740.1"
                     /translation="MTSKQRVVVSRRIFPALVDELRGEFDVLSNQDDVAWNAAELAAA
                     LRDADGALLTAGDRIDEALIAQCPSLKVVANIAVGYNNLDLAACTRHGVLATNTPDVL
                     NEATADHAWALLLAAARRVGESERWVRAGQWKRWTFEMFMGAEVSGTTLGILGMGRIG
                     RAIARRASGFSMSVMYHNRTRLPAELEGDARHVELPELLRESDHLVLVVPYSTAVHHL
                     IDAGALAQMKRTAVVVNIARGGVVDDAALVAALRAGRLAAAGLDVFENEPAPHPGLLE
                     LENVVMTPHIASSTRATRAAMARLAISNLRQALAGQPPPSLLNPDVLQNRRR"
     gene            complement(3461..5146)
                     /locus_tag="VLN25_03875"
     CDS             complement(3461..5146)
                     /locus_tag="VLN25_03875"
                     /inference="COORDINATES: protein motif:HMM:NF009310.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter subunit D"
                     /protein_id="HSQ09741.1"
                     /translation="MSEPLFHPGLLLIAGAVVLPWLRGSVRSAVVLLLPLAAIALVWS
                     VPDGAVWQVRFLGLTLTPLEGSRLGRLFATIFSIMVFGGGLYALGQKSRLELPAALLY
                     AGAAIGVALAGDLVTVFIFWEVMAIGSTLVLWSAGTPASYRASMRYLMIHLGGGVLLF
                     AGVAGHVVQTGDVAFTRMSADSVAHWLILAGFLVNAGAPPLSAWLPDAYPEASWSGTV
                     FLSAFTTKTAVYVLLRGFPGFEVLIWIGLVMVFYGIVYALLENDMRRILAYSIVNQVG
                     FMVAGIGIGTDMALNGAAAHAFTHIIYKALLLMSAGSVLYMTGRRKCSELGGLFHSMP
                     VTTICGTIGALAISSFPLTSGFVSKSMISQAASDGGLLTVWLLLAAASAGVFLHAGIK
                     FPWFVFFQKDSGLRPPDPPTSMKWAMILFAFLCIALGVWTEPLYSMLPYPVDYVPYTA
                     AHVLTQLQLLLFSGLAFFLLLPMLKRTLTITLDTDWVYRRLLPSVVRGADRILGGMGS
                     KLAAAGARRVALVLAGIETRSGPSGVLARTWPIRSMALWVLLLLVAYLVLYYV"
BASE COUNT          994 a         1789 c         1643 g          826 t
ORIGIN      
        1 ggcagcatgc acagcgtcag caccagcttc tcgaattcgg gctcgaagcg gtcgatgatc
       61 cgttgcggtt cttcgcacag gcgcttgtcg gtgatcagtc cgaactggac gccaccggcg
      121 taggacagga tcgacacgcc cacgccgatg ttgccggact gcggcaccca gaccatcatg
      181 cgcttgacct ccgtgccggc catgtaaagc ggctgctgcg gtcccggcac gttggtcatc
      241 accgcggtcg ccttcttcgc caggaagtcg agtgcctgtt tctgcaatcc acgcggcgcg
      301 aaaccgacga caccgagaat gcccatcgac agcacggccg tgtagctctg cttgagttca
      361 tccatccgcc gccgcacttc gtagacgcgc gaaatcggat tggcgatgcc gaccggcaac
      421 aaaagcggca ccagtccgaa cttgttgccc agttccttcc aagtgcgcgg atcgcgcagg
      481 ttgaccggca ccatggctcg cagctcgatg ccatcgactt cttcaccctg atgctcgagg
      541 taggaccgga tcgccccgga cacgcaagac agcagcacat cgttgaccga gcaccccagc
      601 gccttgccga ccgccttcac atcgtcgagg gccagaggct cgttccaggc caccaccttg
      661 gagcctgacg gcagcccctt gaggctggtg accgaatcgt tgtccatcaa ggcgataccg
      721 gcgaagtcct tcgtcacctg cgccgccagt cgcgccatgt cgccggcagc tccactgacg
      781 gagtcagggt gcgcgagcaa cgccgcggac gccttcagcg cgcgcacggc cgcacccccc
      841 gtcgagtcaa tcgctcgcac cgttgcgctc gtcagcggac gcagccaggc atcccatgaa
      901 cctccggagg cagctcggga ccgccgaccc ggttcctcga cgccgccatc ttcgggatcc
      961 tcggaggtca gtgccatcac cacgccgatc agcgcaatgc catcggcgat gcaatgatgg
     1021 atccgcacga tcatcgcctg accgccgttg tagttgtcga cgaggtgcat ctgccacagc
     1081 ggcttggtcg aatcgagcgc ggtggaagcc agccgcccga cgagccgctt cagatccgcg
     1141 gtcgtgccct tgcccggcag cgcggtgcgc gccaggtgca gatcgaggtc gaagttgtcg
     1201 tccagctccc agtagtagcc cgtcgcgtcc tgcacgacgc agctgcggaa gcgcgcgtgc
     1261 gacagcaggc gctcttcgag cagtcggcgc atgcggtcgt agtcgacgtt gccgacgaac
     1321 tcgtagaccc cgacgatcat catcaggttg gcctgcgagt ccatccgcag ccatgccgtg
     1381 tcgaccgacg acatccgctc gcgtctgccc atgttgtctc ccgtgtacgc ccgcgcggca
     1441 cattggccgc ggccgacctt cgagggtcgc tggctatgat acgacgcgct atcctcacgg
     1501 ccacgcgctt cgcctcgacg aacgccggcc cgcacgccca tcggccagac caacaaccca
     1561 ggagacaccg tgagcgacct gaaatcgaaa ttcgaaaagg ccgtcgccga ttcgaagaac
     1621 cttcccgaac ggcccgacaa ccagacgctg ctcaagcttt atgcactgtt caagcaggcg
     1681 tcgaccggcg acgtcgaagg caagcgtccg ggattcaccg acatggtcgg acgcgccaag
     1741 tgggacgcgt ggaacgagat caaggggacc gcgaccgatg acgccatgaa gcagtacgtg
     1801 gcgctcatcg aagacctcaa ggtctgaagc tcgcgcggct tcgagagagt tggccgccgc
     1861 agcacaatca gagtcgcgcg cagtctcgga gcggtgttca gccccgagac gcttttgcca
     1921 gagcgccggg gcgcccgaag cgttcagccc ttgcgcgtcg ctttcttcgc gggcttgacc
     1981 gcgggtttct tgcgagccgc ttccttggcc ggtgccttgg tcgatgcggt tccgggcttc
     2041 tgggccggct tgtgggcagc atgagccttg gcgagcagcc gatcgaagtt gtgctcgatg
     2101 tgctcgacaa acttggcctt gaacgccttc agcaggaagc ccagtgtcac ttcgaggtcg
     2161 atctcggact cggtcacccg catctcgccc tgaacgccac tacgacgaaa gcgcagcgtg
     2221 tttccggccc actggctggt gaggtcgtac tcctcggcca ggtcgtccgc ggcttgttgc
     2281 acaagcgtgc gcgcctgtgc catcggcagc gagtggtact tgatcatcct gatgtcagcc
     2341 atggggcctc cgaaggtcag cggcgccgat tttgcagcac atcggggttc agcagcgaag
     2401 gcggtggctg acccgccagc gcctgtcgca ggttgctgat cgccagtcgc gccatggccg
     2461 cacgcgtcgc gcgggtgctc gacgcaatgt gcggggtcat gacgacattc tcgagttcca
     2521 gcaacccggg atgcggcgcc ggttcattct cgaacacgtc cagcccggcc gctgcgagcc
     2581 gccctgcgcg cagcgccgcc accagcgcgg catcgtcgac cactccacca cgcgcgatgt
     2641 tgacgacgac tgcggtgcgc ttcatttggg ccagggcccc cgcgtcgatc aggtgatgca
     2701 ccgctgtcga gtacggcacg accaggacga ggtgatcgga ctcgcgcaat aactcgggca
     2761 gttcgacatg ccgcgcatcg ccctcaagct ccgccggcaa gcgcgtccgg ttgtgataca
     2821 tcaccgacat cgagaagccg ctcgcccggc gcgcaattgc ccggccgatg cgtcccatgc
     2881 cgaggatgcc gagtgtcgtg ccgctcacct cggcgcccat gaacatctcg aaggtccagc
     2941 gcttccactg gcccgcccgg acccagcgtt cggattcgcc gacccgccgg gccgccgcca
     3001 gcagcagggc ccatgcgtgg tcggccgtcg cttcgttcag cacgtcgggc gtattggtgg
     3061 ccagtacacc gtggcgcgtg cacgccgcaa ggtcgaggtt gttgtagccg accgcgatgt
     3121 tggccaccac tttcaggctc ggacattgtg cgatcagcgc ttcgtcgatg cggtcaccgg
     3181 cggtcaacag cgcaccgtct gcgtcgcgaa gcgccgccgc cagttcggcg gcgttccacg
     3241 cgacatcgtc ctggttcgac agcacgtcga actcaccgcg aagctcgtcc accaatgccg
     3301 ggaagatgcg ccgtgatacg accacgcgtt gtttcgatgt catcgtgcga ctcgccgtac
     3361 gagagtcgta acacgaaaca gggtcatctt cggtccgatt aaacaggttg gaagcaaagg
     3421 gttggaggct cgatcactcg tgaacgcagt caggcgcagc tcagacgtag tacagcacga
     3481 gataggccac cagcagcagc agaacccaca gcgccatgct gcggatcggc cacgtgcgcg
     3541 caagtacgcc cgacggcccg gatcgggtct cgatgccggc cagcaccagc gcaacccgtc
     3601 gcgcgcccgc cgcggccagc ttcgagccca tgccaccgag gatgcgatcc gcaccccgca
     3661 cgacggacgg cagcaggcga cgatagaccc agtcggtgtc cagcgtgatg gtcaatgtcc
     3721 gtttcagcat cggcaagagc aggaagaacg ccagccccga gaagagcagg agctgcagct
     3781 gcgtcagcac atgagcggcc gtgtagggca cgtagtcgac tggatacggc agcatgctgt
     3841 agagcggctc ggtccacacg cccagcgcga tgcacaggaa tgcgaacagg atcatggccc
     3901 acttcatgga cgtcggcggg tccggcggcc gcaggcccga gtccttctgg aagaacacga
     3961 accacgggaa cttgatgccc gcgtgcagga agactccggc cgacgcggcg gcaagcaaca
     4021 gccagaccgt gagcaatccg ccgtcgctgg ccgcctggct gatcatcgac ttcgacacga
     4081 agcccgaggt gagcgggaat gacgagatcg ccagcgcgcc gatcgtcccg cagatcgtcg
     4141 tgaccggcat gctgtggaac aggcccccca gttccgaaca cttgcgccgt cccgtcatgt
     4201 agagcaccga acctgccgac atcagcagca gtgccttgta gatgatgtgc gtgaacgcat
     4261 gcgcggcggc gccgttcaat gccatgtccg tgccgatgcc gatgccggcg accatgaacc
     4321 ccacctggtt cacgatcgag taagcgagga tgcggcgcat gtcgttttca agcagcgcgt
     4381 acacgatgcc gtagaacacc atgacgagac cgatccagat cagcacctcg aatcccggga
     4441 agccgcgcag caggacatac acggcagtct tggtcgtgaa cgccgacagg aataccgtcc
     4501 cgctccacga cgcctccgga tacgcatcgg gcagccaggc cgacagcggc ggcgcacctg
     4561 cgttgacgag aaacccggcc aggatcagcc agtgcgcaac cgaatccgcg ctcatgcgcg
     4621 tgaacgcgac gtcgcctgtc tggaccacgt ggccggccac gccggcaaag agaagcacgc
     4681 ccccgccgag gtgaatcatc aggtagcgca tgctggcgcg atacgacgcc ggcgtgcccg
     4741 cgctccacag caccagcgtc gaaccgatcg ccatcacttc ccagaagatg aacacggtga
     4801 cgaggtcgcc cgccagcgcc acaccgatgg cggcaccggc gtacagcagc gcggccggga
     4861 gttcgaggcg gctcttctgc cccagtgcat acaggccgcc gccgaacacc atgatcgaga
     4921 agatcgtggc gaacaggcgc ccgagccggc tgccttcgag cggtgtcagg gtgaggccga
     4981 ggaagcgcac ctgccagacg gcaccgtcgg gaaccgacca caccagggcg attgcagcca
     5041 gcggcaacag cagcactaca gcggatcgca ccgaaccgcg cagccacggc aggacgaccg
     5101 cgcccgcgat cagcaggagt ccgggatgga aaagcggttc actcatcgcg cgagtgctcg
     5161 ccgtcgtagt acgtatcggg ccgtttgagc agcacgccga gcgccttcgc gacgccgatc
     5221 atcgcggcgc aggccagcag gccataccag gc
//
LOCUS       DATIRT010000164         2596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_711318, whole genome shotgun sequence.
ACCESSION   DATIRT010000164 DATIRT010000000
VERSION     DATIRT010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711318"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VLN25_03880"
     CDS             <1..972
                     /locus_tag="VLN25_03880"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HSQ09742.1"
                     /translation="LVDRRMARARTITVKAQHLVAAVVLLTLSIIVVSTVFSLLSVRA
                     ASALPLPFVSDVIAFVTRDQAERKEQYVRDSVSAMTRQLGEVQAQLLRLDVLGERVAR
                     AAGIRPEEFRFRELPGRGGAPGSTMSFTDLDSELQGLTRGVEQRADYLAVVEAELRSQ
                     QVRRALLPGSQPVADGYIGSGYGWRTDPFTREMTRHDGIDFAAPQGTPIHAAAGGVVV
                     VAELHPVWGKVVEIDHGNQLMTRYAHASRLTVVAGELVKRGHKIAEVGSTGRSTGPHL
                     HFEVHSKGVAQNPAKFLFADGNPLQQHAASSARIDLKALANAAAARN"
     gene            1107..>2596
                     /gene="secA"
                     /locus_tag="VLN25_03885"
     CDS             1107..>2596
                     /gene="secA"
                     /locus_tag="VLN25_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HSQ09743.1"
                     /translation="MISGFLTKIFSSRNERLLKTYERNVERINALEAQVEALTDDALR
                     AKTDEFRGRVAGGETIEALLPEAFAVVREAAKRTLGMRHFDVQLVGGMVLDAGKIAEM
                     RTGEGKTLVATLPAYLNALTGKGVHIVTVNDYLASRDADWMGKVFTFLGMSVGVILSQ
                     QSTEDKRAAYAADITYGTNNEFGFDYLRDNMEYSVVDRRQRGLNYAIVDEVDSILIDE
                     ARTPLIISGPAEDHTETYRRINEVPPLLTRQKDEKGDGDYWVDEKAHQVHISESGHEK
                     LEQILQRLGLLTEGESLYAPGNIILMHHLNAALRAHTLFHRDQQYVVQNDEIIIVDEF
                     TGRLMPGRRWSEGLHQAVEAKEGVKIQAENQTYASITFQNYFRMYGKLAGMTGTADTE
                     AYEFQEIYRLETVVIPTHRPMIRIDSQDKVYRTLKEKYSAIIEDIKDCYERGQPVLVG
                     STSIESSELLSNLLKQEKLPHQVLNAKQHAREAEIVAQAGRPKVIT"
BASE COUNT          491 a          822 c          843 g          440 t
ORIGIN      
        1 ctggtcgatc ggcgaatggc gcgagcacgc acgatcacgg tcaaggcgca acacctggtc
       61 gcggcggtcg tcctgcttac gctgtcgatc atcgtcgtgt ccaccgtctt ttccctcttg
      121 tccgtccgtg cggcctcggc gctgcccttg cccttcgttt ccgatgttat cgccttcgtg
      181 acgcgcgatc aggccgagcg caaggagcag tacgtgcgcg acagcgtttc ggccatgacg
      241 cgccagctcg gcgaagtgca ggcgcagttg cttcggctcg atgtgctcgg cgaacgcgtg
      301 gccagggccg ccggcatccg gcccgaagag ttccgtttcc gcgagttgcc gggacggggc
      361 ggcgcgccgg gaagcaccat gagcttcact gatctagatt ccgaactgca gggcctgacc
      421 cgcggagtcg agcagcgcgc tgactatctg gccgtggtcg aggcggagct gcggtcgcaa
      481 caggtacgtc gggcgttgct gcctggcagc cagccggtgg cggatggtta catcggctcg
      541 ggctacggct ggcgcaccga cccgttcacc cgtgaaatga cccggcacga cggtatcgat
      601 tttgccgcgc ctcagggcac tccgatccat gcagcggccg gcggcgtcgt ggtcgtggcc
      661 gagcttcatc cggtctgggg caaggtggtc gagatcgacc acggcaacca gttgatgacg
      721 cgctatgcgc acgccagccg tctgaccgtc gtggccgggg aactggtcaa gcgcggtcac
      781 aagattgccg aggtgggaag cactggtcgt tccaccggtc cgcaccttca tttcgaagtt
      841 cacagcaagg gcgttgcgca gaacccggcg aagttcctgt tcgccgacgg caatccgctg
      901 cagcagcatg ccgccagttc ggcgcgcatc gacctgaagg cccttgcgaa cgccgcggcc
      961 gcccgcaact aggcctccag gagtcacccg ggcggccgat ccccctgtga cataattttc
     1021 gctttgcgca atgtggtcgg gccctcatcg gccgccgcag gcaatcacct ctgggcgtcg
     1081 acgcgcaagc gcgacccgtt tcccgcatga tttccggttt cctgaccaag atattcagca
     1141 gtcgcaacga gcggctgctc aagacctacg aacgcaacgt cgaacgcatc aatgcgctcg
     1201 aggctcaggt cgaggcgctc accgacgatg ccctccgcgc gaagacggac gagttccgtg
     1261 gccgggtggc cggcggagag accatcgaag ccctgctgcc ggaagcgttt gcggtcgtgc
     1321 gcgaggcggc caagcgaacg ctcggcatgc gccactttga cgtgcaactg gtcggcggca
     1381 tggtgctgga cgccggcaag attgccgaga tgcgcacggg cgagggcaag acactggtgg
     1441 ccacgctgcc cgcttacctc aatgcgctga cgggtaaggg cgtgcacatc gttacggtca
     1501 acgactacct ggcgagccgc gacgccgact ggatgggcaa ggtcttcacc tttctgggca
     1561 tgtcggtcgg cgtgatcctg tcgcagcagt cgaccgagga caagcgcgcg gcttatgcgg
     1621 ccgacatcac ctacgggacg aacaacgagt ttggcttcga ttacctgcgc gacaacatgg
     1681 agtactcggt cgtcgaccgg cgccagcgcg gcttgaacta cgcgatcgtc gacgaagtcg
     1741 actcgatcct gatcgacgag gcacgcacgc cgctgatcat ttccggcccg gccgaggatc
     1801 acaccgaaac ctatcgccgc atcaacgagg tcccgccgct gctcacgcgg cagaaggacg
     1861 agaagggcga tggtgactac tgggtcgacg agaaggccca ccaggtccac atctcggagt
     1921 cgggccacga gaagctcgaa cagatcctgc agcggctggg actgctgacc gagggcgaga
     1981 gcctttatgc gcccggcaac atcatcctga tgcaccacct gaacgccgcg ttgcgagcgc
     2041 acacgctgtt ccatcgtgac cagcagtacg tggtgcagaa cgacgaaatc atcatcgtcg
     2101 acgagttcac cggccggctg atgccggggc gccgctggtc cgagggcctg caccaggccg
     2161 tcgaagccaa ggaaggcgtg aagatccagg cggagaacca gacctacgca tcgatcacgt
     2221 tccagaacta cttccgcatg tacggcaagc tcgcgggcat gacgggcacg gccgacaccg
     2281 aagcctatga attccaggag atctaccggc ttgagacggt cgtcatcccg acgcaccggc
     2341 cgatgatccg tatcgattcg caggacaagg tttatcggac gctgaaagag aagtactcgg
     2401 cgatcatcga ggacatcaag gactgctacg agcgcggcca gccggtgctg gtgggatcga
     2461 cctcgattga gagctccgag ctgctttcga acctgctcaa gcaggaaaag ctgccgcatc
     2521 aggtgctcaa tgcgaagcag catgcccggg aggccgagat cgtcgcgcag gcgggtcgtc
     2581 cgaaggtgat cacgat
//
LOCUS       DATIRT010000165        10923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_514201, whole genome shotgun sequence.
ACCESSION   DATIRT010000165 DATIRT010000000
VERSION     DATIRT010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10923
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514201"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..1425)
                     /gene="phoR"
                     /locus_tag="VLN25_03890"
     CDS             complement(82..1425)
                     /gene="phoR"
                     /locus_tag="VLN25_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HSQ09744.1"
                     /translation="MVDRLKVSLSVLGRPLLRLATLAVVGAIVGFFFGTVAALWTVII
                     GLVGLLAIHMSYASRLAAWLEAPKLDAIPNGWGLWTDVFARLYRQRRATEINERRLLE
                     NEERFRRTISALPEGIVLADASLQIDWCNPVAEEHLGIRLTADHGLRVTNLVRDPEFI
                     NYMTSSHFEAPLVFRPLSRPGIALEVRVVDFEPSRSIIITRDITQRERVDAVRRDFIA
                     NVSHELRTPLTVVTGFLEMLLDAQTEDPATRRHHLGLMHEQAQRMTRLVEDLLTLSRL
                     ESRESLVTEDVVDIRQLVREVGDEARVLSNGRHRVEVETPPAFVRGSRDELRSAFGNL
                     VSNAIRYTPEGGQIVVRWVESGGEGRFEVEDTGIGIAPEHLARLTERFYRVDKSRSRE
                     TGGTGLGLAIAKHVLMRHDGRLDINSEIGKGSTFSAVIPGERLISVVDHGMAQAA"
     gene            complement(1526..2221)
                     /gene="phoB"
                     /locus_tag="VLN25_03895"
     CDS             complement(1526..2221)
                     /gene="phoB"
                     /locus_tag="VLN25_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018573660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HSQ09745.1"
                     /translation="MSTNILIVEDEPAIQELVAYTCSSNGYSVRRAESVSAAREAIGQ
                     QLPDLVLLDWMLPDKPGIDLLRDLRTEDRTRAVPVIMLTARGSEADRVVGLDTGADDY
                     VVKPFSPRELVSRIRAVFRRRAPQHSGESVSYGPLTIDPTRHEVLVEGNPVSMGLSEF
                     KLLSFLVNHPDRVYSRTQLLDNIWGDHVFIEERTVDVHVLRLRKALTPARAQHLVQTV
                     RGLGYRLSMRPEH"
     gene            complement(2228..2941)
                     /gene="phoU"
                     /locus_tag="VLN25_03900"
     CDS             complement(2228..2941)
                     /gene="phoU"
                     /locus_tag="VLN25_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HSQ09746.1"
                     /translation="MGTGDHSMRKYDEELESMRSRVLQMGGLVESQVRTALDAFETAN
                     VELAQQAIDADRRVNELEMDLDQMVNHVIARRQPTAGDLRMITGIAKAITDIERIGDE
                     ASKIARAAKWLREKDGQSRVNRVPDIRYSGEAAVSMLRRALDAFARLDALAAASIIKD
                     DQGVDDRFRAILRQLITFMMEDPRTISASLDTVWAAKAIERIGDHSKNIAEHVIFISQ
                     GVDARHLSPDELERAIARG"
     gene            complement(2975..3763)
                     /gene="pstB"
                     /locus_tag="VLN25_03905"
     CDS             complement(2975..3763)
                     /gene="pstB"
                     /locus_tag="VLN25_03905"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HSQ09747.1"
                     /translation="MHAATSALKPGEKVKMTVRDLNFYYGKFQALKNINLEIPEKKVT
                     AFIGPSGCGKSTLLRVFNRMFELYPDQRAEGEVSVDGENVLTSKEDIALIRAKIGMVF
                     QKPTPFPMSIYDNIAFGVKLFENLSKSALDDRVEWALRKGALWNEVKDKLGQSGTSLS
                     GGQQQRLCIARGIAVRPEIILFDEPCSALDPISTAKIEELIDELKSDYTVAIVTHNMQ
                     QAARVSDYTAYMYLGDMVEFGETDQIFIKPKKKETEDYITGRFG"
     gene            complement(3773..4648)
                     /gene="pstA"
                     /locus_tag="VLN25_03910"
     CDS             complement(3773..4648)
                     /gene="pstA"
                     /locus_tag="VLN25_03910"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HSQ09748.1"
                     /translation="MIAVPQAVNADYRLPLHRRRKLINVIALALSLAAMAFGLVWLVW
                     ILWDTLWLGVQGLSASIFLEDTPPPNSDGGGLRNAIVGTLVMVLSATAVGTPIGILAG
                     IYLAEYGQRGWLGSVTRFINDILLSAPSIVIGLFVYTIIVAQTRTFSAYAGVIALALI
                     VIPVVIRTTENMLALIPNALREATFALGAPKWIMIRRVTLKAARAGVVTGVLLALARI
                     AGETAPLLFTALSNQFFTLNMGEPMANLPVTIFKFAMSPFSNWQQMAWVAVFLITVGV
                     LVLNILARVLFRQKY"
     gene            complement(4645..5604)
                     /gene="pstC"
                     /locus_tag="VLN25_03915"
     CDS             complement(4645..5604)
                     /gene="pstC"
                     /locus_tag="VLN25_03915"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HSQ09749.1"
                     /translation="MPEEPPARRSGRLGDVIFLNLTRGAAILTLLLLAGILLSLVIGA
                     WPSIREFGLAFLWTAEWDPVQEKFGGLVMIYGTLMTSLIALIIAVPVSFGIALFLTEL
                     SPKWLKRPLGTAIELLAAIPSIVYGMWGLLVFAPILSKYVQQPLQAVFSNVPVLGSLF
                     SGAPVGIGILSAGIILAIMIIPFIAAVMRDVFEVTPPMLKESAYGVGATTWEVVWRVV
                     LPYTKAGVVGGIMLGLGRALGETMAVTFVIGNFNQLNSLSLFEAANSITSALANEFAE
                     ATPGLHEASLIYLGLILFFITLVVLAFSKYLLLQLKKREGSRT"
     gene            complement(5750..6787)
                     /gene="pstS"
                     /locus_tag="VLN25_03920"
     CDS             complement(5750..6787)
                     /gene="pstS"
                     /locus_tag="VLN25_03920"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HSQ09750.1"
                     /translation="MRLLPIKALAASIALVGVVSTGIAQEITGAGATFPAPLYAKWAD
                     AYNKATGARINYQSVGSGAGMRQIRGKTVDFGASDAPLNDADLAKDGLIQFPTVIGGV
                     VPVINIQGIQSREMRLTGQVLGDIYLGKITKWNDPAIAALNPGVKLPDAAISPVRRAD
                     GSGTTFIFTNYLSKVNPEWKAKVGEGTAVNWPVGAGGKGNEGVSAFVQRLPNSIGYVE
                     YAYAKQNKIPYVLMQNAAGTWVAPDDLTFKAAAAGAEWQKSFNQVLTNQAGKEAWPIT
                     GATFIMMHTKQDKPQQAAASLKFFDWAYANGDKMADDLDYVALPDSVKALIRQQWAKI
                     GDAAGKPVLGK"
     gene            7088..7561
                     /locus_tag="VLN25_03925"
     CDS             7088..7561
                     /locus_tag="VLN25_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HSQ09751.1"
                     /translation="MNRRTWLLGLLAVSLAGCASMQPKNVADVIASDSELSTLNKLIN
                     DAGLTQTLSGTGPFTVFAPSNAAFKAVPAKTMGELATNKALLTQVLTFHVVPAKAMSA
                     DVKQGNLKSVNGANLGLAKAGNYVTVESAIVTRPDLAASNGVVHVVDSVLLPPTR"
     gene            complement(7639..8703)
                     /gene="arsB"
                     /locus_tag="VLN25_03930"
     CDS             complement(7639..8703)
                     /gene="arsB"
                     /locus_tag="VLN25_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HSQ09752.1"
                     /translation="MTEAVALPTPAAAMSLFERYLTVWVALCIVAGIAIGQLLPGPVQ
                     VIGTMEIARVNLPVGVLIWVMIIPMLLRIDFAALGQVRRHVRGIGVTLFINWLVKPFS
                     MALLAWIFIRQLFAPWLPADQIDSYIAGLILLAAAPCTAMVFVWSRLTHGDPYFTLSQ
                     VALNDTIMIFAFAPIVALLLGVASITVPWDTLLTSVALYIVVPVIIAQLIRAYVLARG
                     GPAALNALLARLGPWSIAALLATLVLLFAFQGEQILRQPLIIAMLAVPILIQVFFNSA
                     LAYWLNRAVGEKHNVACPSALIGASNFFELAVAAAISLYGFESGAALATVVGVLIEVP
                     VMLLVVRVVNRSKGWYEQRA"
     gene            complement(8700..9200)
                     /locus_tag="VLN25_03935"
     CDS             complement(8700..9200)
                     /locus_tag="VLN25_03935"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HSQ09753.1"
                     /translation="MNVLFLCTGNSARSIMSEVMMNAMAKGRFIAHSAGSMPTGTVNP
                     FAIELLQKSRLPTEGLRSKSWDEFAAAGAPPLDFVFTVCDNAAGEVCPMWPGQPVTAH
                     WGVEDPAAIEGGDEDKRKAFFRAFTTLQRRIELFLSLPLAKLDKLSLQQRLNEIGRSG
                     ATQSAA"
     gene            complement(9225..9707)
                     /locus_tag="VLN25_03940"
     CDS             complement(9225..9707)
                     /locus_tag="VLN25_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HSQ09754.1"
                     /translation="MKRFHVHVSVPDLDSSIRFYSTLFGTEPAVVKGDYAKWMLEDPR
                     VNFAISQRGGKPGLNHLGLQTDSDGELESLNAQLQRADLATQAEKEASCCYARSNKYW
                     VTDPTGIAWETFHTMGSVPTFNGSDTEVTTQATGCCAGDATAAMPTSARAKAASACCS
                     "
     gene            complement(9704..10090)
                     /locus_tag="VLN25_03945"
     CDS             complement(9704..10090)
                     /locus_tag="VLN25_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HSQ09755.1"
                     /translation="MESITVVRALGALAQETRLGVFRLLVEQGPSGMAAGEIAEELTI
                     APATLSFHLKELTHAGLVTARHEGRFIFYAANFTAMNDLLAYLTDNCCVADCGPGALC
                     APLAACSPAPPKRATAGRRKPQRSKA"
     gene            complement(10211..10843)
                     /locus_tag="VLN25_03950"
     CDS             complement(10211..10843)
                     /locus_tag="VLN25_03950"
                     /inference="COORDINATES: protein motif:HMM:NF014651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HSQ09756.1"
                     /translation="MLTDEVRLLAARADLARLLSASYYEPGEEFAEEQLFASVREAAS
                     SIDPALGAVAQRLESAYAEADRQELLIDYVRLFHGPPRALAQPYASVWLSMTTQTVME
                     DSTVAVMELYREAGFEIAEDFRDLPDHVSVELECLYALLFREAQAGRDGQTDKQLGAK
                     ALRRRLLKEHLAGWLAQFAESVIAQAQTPFYRTLAELTRDYIDFERSAQS"
BASE COUNT         2081 a         3519 c         3327 g         1996 t
ORIGIN      
        1 agcaggcgcg ctaccagact gcgctacacc ccgagggcgg agcgatgata acacccgtcc
       61 tccaatgatc gtcactcacc gtcacgccgc ttgcgccatg ccgtgatcaa ccacggagat
      121 caggcgctcg ccgggaatga cggcactgaa cgtgctgccc ttgccgatct cggagttgat
      181 gtcgagccgc ccgtcgtggc gcatcaggac gtgcttggcg atggccaggc caagtcccgt
      241 tccaccggtt tcacgcgagc ggctcttgtc gacccggtag aagcgctcgg tcagccgcgc
      301 caggtgttca ggcgcgatgc cgatgccggt gtcctcgacc tcgaagcgcc cttcgccacc
      361 ggactcgacc cagcgcacga cgatctggcc accttccggc gtgtagcgga tcgcattgga
      421 cacgaggttg ccgaacgcac tgcgcagttc gtcgcggctg ccgcggacga atgccggcgg
      481 agtttcgacc tcgacgcgat gccggccatt ggacaggact cgggcctcgt ctccgacctc
      541 gcgaacaagt tggcgtatgt cgaccacgtc ttcggtgacg aggctttcgc gtgattcgag
      601 ccgagacagc gtcagcaggt cttcgaccag tcgtgtcatg cgctgcgcct gttcgtgcat
      661 cagtccgagg tgatggcgtc gcgtggcggg atcctcggtc tgcgcatcca gcaacatttc
      721 gaggaaaccg gtaaccaccg tcagcggggt acgcagttcg tgcgaaacat tcgcgatgaa
      781 gtcgcgccgc accgcgtcga cgcgttcgcg ctgggtgatg tcgcgcgtga tgatgatgga
      841 acgagacggc tcgaaatcga ccacgcgcac ttccagcgca atgcccggtc gcgagagcgg
      901 gcgaaacacc agcggcgcct cgaagtggga agaggtcata tagttgatga actccggatc
      961 acgaacgaga ttagtgaccc gcaagccatg gtcggccgtc agtcggatgc cgaggtgttc
     1021 ctcggccact gggttgcacc agtcaatctg caacgaggca tcggcgagca cgatgccttc
     1081 cgggagggcg ctgattgtcc gccggaaccg ttcctcgttt tcgagcaggc gacgttcgtt
     1141 gatctcggtg gctcggcgct gccggtacaa ccgggcgaac acatcggtcc agaggcccca
     1201 cccgttggga atcgcgtcga gcttgggagc ctcgagccag gccgccagcc ggctcgcgta
     1261 gctcatgtgg atggccagga ggccgaccag cccgatgatg accgtccaca gagcggctac
     1321 ggttccgaag aagaaaccga cgatggcacc gacgaccgcc agcgtcgcca atcgaagcag
     1381 cggccgcccg agcacagaca aacttacctt caggcgatca accatggaag cagtctagtc
     1441 cgtgggtgtg gcaggaggct tggctcagcg cagccccggg taagtcggct ggccgtcggg
     1501 cccctacccc gccgtcgtgc aggcttcaat gttccggacg catcgacagg cggtagccca
     1561 gcccgcgcac ggtctgcacc agatgctgtg cacgggccgg cgttagggcc ttgcgcagcc
     1621 gcaggacgtg cacgtcgacc gtgcgctcct cgataaacac gtgatcaccc cagatgttgt
     1681 cgagcaactg cgtgcgtgag tacacgcgat caggatgatt gacgagaaag ctcagaagct
     1741 tgaactccga cagtcccatg ctcaccgggt tgccctcgac cagcacttcg tgccgcgtcg
     1801 gatcgatggt caacggcccg tagctgaccg attcgccact gtgctgcggt gcgcggcgac
     1861 ggaacacggc gcggatgcgt gacaccagct cgcgcggcga aaacggcttg actacatagt
     1921 cgtcggcacc cgtgtcgagc ccgacgaccc gatcggcctc gctgccgcgc gctgtcagca
     1981 tgatgaccgg taccgcgcga gtacggtcct cggtgcgcaa atcccttagc agatcgatgc
     2041 cgggcttgtc cggcagcatc cagtcgagga gcacaaggtc gggaagctgc tgaccgatcg
     2101 cctcgcgcgc cgcgctcacc gactcggcac gtcgaacgga gtatccgttg ctgctgcagg
     2161 tataggcgac cagttcctgg atcgcgggtt cgtcttcgac tatgagtatg ttggtggaca
     2221 tccgggatca gccacgtgcg atcgcgcgtt ccagttcatc cggcgacagg tggcgcgcat
     2281 cgacaccctg cgagatgaag atcacgtgct ccgcgatgtt cttcgagtga tcaccgatgc
     2341 gctcgatcgc cttggccgcc cacaccgtgt cgagcgacgc ggaaatcgtg cgtgggtctt
     2401 ccatcatgaa ggtgatcagc tggcgcagga tcgcgcgaaa gcggtcatcg acaccctggt
     2461 catccttgat gatcgaggca gccgctaggg cgtccagtcg agcgaacgca tccagcgccc
     2521 ggcgcagcat cgacaccgct gcctcgcccg agtaacggat gtcgggcacg cgattcaccc
     2581 gcgactggcc gtccttctcg cgtaaccact tcgccgcgcg cgcaatcttg ctcgcctcgt
     2641 cgccgatgcg ctcgatgtca gtgatcgcct tggcgatccc cgtgatcatc ctcaggtcac
     2701 cggccgtcgg ctggcgccgc gcgatcacat ggttgaccat ctggtcgaga tccatttcga
     2761 gctcgttaac ccgccgatcg gcgtcgatgg cctgctgcgc cagctcgacg ttggcggtct
     2821 cgaacgcatc cagcgcagtg cgcacctgcg attcgacaag gccacccatc tgcagcacgc
     2881 gcgaacgcat ggattcgagt tcctcgtcgt atttcctcat cgagtgatca ccggttccca
     2941 tttcactctc ccgcggcccc gccgcaatgc tgcactagcc gaagcggcca gtgatgtagt
     3001 cctcggtttc cttcttcttc ggcttgatga agatctggtc ggtttcaccg aattcgacca
     3061 tgtcgccgag gtacatgtag gccgtgtagt cggacactcg tgcggcctgc tgcatgttgt
     3121 gcgtcacaat cgccacggtg tagtctgact tcaattcgtc gatcagttcc tcgatcttcg
     3181 cggtagaaat cgggtcaagg gccgaacatg gctcgtcgaa caggatgatc tcaggacgca
     3241 cagcgatgcc gcgcgcgatg cacagtcgct gctgctggcc gcccgacagg ctggtgccgc
     3301 tctgcccgag cttgtccttg acctcgttcc acagcgcgcc cttgcgtagc gcccactcga
     3361 cccgatcgtc cagcgctgac ttcgacaggt tctcgaacag cttcacgccg aacgcgatgt
     3421 tgtcgtagat cgacatggga aacggcgtcg gcttctggaa caccatcccg atcttggcac
     3481 ggatcagcgc gatatcctcc ttcgacgtca gcacgttctc gccgtcgacg ctgacttctc
     3541 cctcggcgcg ctggtccgga taaagctcga acatccggtt gaatacgcgc agcaacgtcg
     3601 acttgccgca cccggatggc ccgatgaacg cggtgacctt cttctcggga atctccaggt
     3661 tgatgttctt cagcgcctgg aacttcccgt agtaaaagtt caggtcgcgc accgtcatct
     3721 tgaccttctc gcccggcttc aacgcgctcg ttgcggcgtg catggtggac tcctagtact
     3781 tctgtctgaa tagcacccgc gccaggatgt tcagcacgag cacgccaacg gtgatgagaa
     3841 agacggcaac ccaggccatc tgctgccagt tcgagaacgg gctcatcgca aacttgaaga
     3901 tggtgaccgg caggtttgcc atcggttcac ccatgttcag cgtaaagaac tggttcgaca
     3961 gcgccgtaaa tagcagcggc gccgtctcgc ccgcgatgcg ggccagcgcc agcaggaccc
     4021 cggtcacgac accggcccgt gcggccttca gcgtcacgcg ccggatcatg atccacttcg
     4081 gcgcacccag ggcgaatgtc gcttcgcgca gcgcattcgg gatcagcgcc agcatgttct
     4141 cggtcgtgcg tatcacgaca gggatcacga ttaatgcgag tgcaatgaca ccagcgtagg
     4201 cagaaaaagt cctcgtctgc gccacgatga tcgtatagac gaaaagtcca atcacgatcg
     4261 acggtgctga cagcagaatg tcgttgatga atcgcgtcac gctacccagc cagccccgct
     4321 gcccatactc ggcgaggtag atgccggcga ggattccaat cggggttccg acggccgtgg
     4381 cggaaagcac catgacaagc gtacccacga tggcgttgcg cagtcccccg ccgtccgaat
     4441 ttggcggcgg agtgtcctcc aggaagatcg aggcgctcag accctggacc cccagccaaa
     4501 gggtgtccca cagtatccag accagccaga ccaggccgaa cgccatcgcc gccagcgaca
     4561 aggccagcgc gatgacgttg atcagtttgc ggcgccggtg caggggcagc cggtagtcgg
     4621 cgttcacggc ctgagggaca gcgatcatgt tctcgacccc tcgcgcttct tcaactgcag
     4681 cagcaggtac ttgctgaatg ccagtaccac cagggtgatg aagaaaagaa tcaagcccag
     4741 gtagataagc gatgcctcat gcaggccggg tgtcgcctcg gcaaactcat tggccagtgc
     4801 cgaagtgatc gaattggcgg cctcgaacag tgaaagactg ttgagctggt tgaagttgcc
     4861 gatgacgaac gtcactgcca tcgtctcgcc aagcgcacgc cccagtccga gcatgatgcc
     4921 gccaaccacg ccggccttcg tgtacggcag cacgaccctc cacaccactt cccaggtcgt
     4981 ggcgccgaca ccgtaggcgg attccttgag catcggcggc gtgacctcga acacgtcccg
     5041 catgaccgcc gcaatgaagg gaatgatcat gatcgccaga atgattcccg ccgacaggat
     5101 gccaatgcct accggcgcgc ccgaaaacag cgacccaagt accggaacat tgctgaagac
     5161 cgcttggagc ggctgctgca cgtacttgga caggatggga gcaaaaacca gcaggcccca
     5221 catgccgtag acgatcgacg gaatcgctgc gaggagttcg atcgccgtgc ccaacgggcg
     5281 cttcagccat ttcggagaca gttcggtcag gaatagcgcg atgccgaaac tgaccggtac
     5341 ggcgatgatc agcgcgatca acgacgtcat cagcgtgccg tagatcatga ccaggccgcc
     5401 gaacttttcc tgcactggat cccattcggc tgtccacagg aacgctaggc cgaattcgcg
     5461 gatcgacggc catgcgccga tcacgagcga aagaaggatt cccgcgagca ggagcagcgt
     5521 cagaattgct gccccacgcg tcaggttgag gaatatgacg tcgccaagtc tgccggaccg
     5581 ccgggccggt ggctcctcgg gcatctgcac agtctgggct tctttgcgaa aactggtctc
     5641 ggacagtgta gcgtccatcg aagcgctccc cggcagagcc gaagcagtcc tggtacggtg
     5701 atcgtcgaga aaggaccccg acggcatggc cgccggggac gctagagcgc tacttcccca
     5761 gtaccggctt tccggcggcg tcgccgatct tggcccactg ctggcggatc agcgccttca
     5821 cgctatccgg cagcgccacg tagtccaggt cgtcggccat cttgtcgcca ttcgcatagg
     5881 cccagtcgaa gaacttgagt gatgcagcag cttgctgcgg cttgtcctgc ttggtgtgca
     5941 tcatgatgaa cgtcgcaccg gtgatcggcc acgcctcctt gccggcctgg tttgtcagga
     6001 cctgattgaa ggacttctgc cattccgcgc cggccgcggc ggccttaaag gtcagatcat
     6061 ccggggccac ccaggtgccc gccgcgtttt gcatcagcac gtacgggatc ttgttctgct
     6121 tggcgtaggc gtactcgacg tagccgatcg agttcggcaa ccgctgcacg aacgccgata
     6181 cgccttcatt gcccttgccg cccgcgccca ccggccagtt gacggccgta ccttcgccga
     6241 ccttcgcctt ccactcgggg ttcactttgg acagatagtt cgtgaagata aacgtcgtac
     6301 cggagccgtc ggcgcggcgg actggcgaga tcgccgcgtc cggcaacttc acgcccggat
     6361 tcagcgcggc gatggctgga tcgttccact tcgtgatctt gccaagatag atatccccga
     6421 gcacctgtcc cgtcaggcgc atctcgcgcg actggatgcc ctggatgttg atcaccggca
     6481 ccacgccgcc gatgacggtc gggaactgga tcaggccatc cttcgcgagg tcggcatcgt
     6541 tcagcggtgc gtcggacgca ccgaagtcca cggtcttgcc gcggatctga cgcatacccg
     6601 cgccggaacc caccgactgg tagttgatgc gcgcgcccgt cgccttgttg taggcgtcgg
     6661 cccacttggc atacaacggc gcggggaacg tggcgcccgc accggtaatc tcctgcgcaa
     6721 ttcccgttga aacgacgccg accagcgcaa tgctggcggc cagtgctttg atcggtagaa
     6781 gtctcatgcg aattacctca gtagtggttc gaggaaagga cgagtaggcg gggaagcaag
     6841 gatggccttc aatgccggcg agtctaggaa aggtggatga cagatcggtg acatgtcact
     6901 cactttcggc gttctagagg gaaattccca gcacgacagg cctttgagac tccggttgtc
     6961 ataccgggga agctcatgtc acgccgcagt catgcggccg acgtagcttg ccggaccaat
     7021 gtcagcggtt cggggcgacc cctgttcgct tggcattccg atccttttct actgcccagg
     7081 agtctgtatg aatcgtcgta cttggctgct cggcctgctt gcggtttcgc ttgccggctg
     7141 tgcgtcgatg caaccgaaga atgtggccga tgtcatcgcg tcggattccg aactgtccac
     7201 tctgaacaaa ctcatcaacg atgccgggtt gacccagact ctgtccggca ccggcccgtt
     7261 cacggtgttc gcccccagca atgccgcgtt caaggcagtc ccggcgaaga cgatgggtga
     7321 actcgccacc aacaaggcgt tgctcactca ggtcctgacg tttcacgttg tcccggccaa
     7381 ggccatgtcg gccgatgtca agcaaggaaa cttgaagtcg gtcaacggcg cgaatctggg
     7441 gctggccaag gccgggaact acgtgaccgt cgagagtgcg atcgtcaccc ggcccgatct
     7501 ggccgccagc aacggggtcg tacacgtcgt cgacagcgtc ctgctgcctc cgacgcgcta
     7561 gacgccactc tgttgcacga gaagctcgac gaatagcggc gcccgcgagc gccgcttctt
     7621 ctcactgcgc acaactcctc aggcacgctg ctcgtaccac cccttgctgc gattcacgac
     7681 ccttacgacc agcagcatca ccggcacttc gatcagaacg ccgaccaccg tcgccagcgc
     7741 cgctccggat tcgaaaccat acaggctgat cgccgcagcc acggccagtt cgaagaagtt
     7801 cgaagcgccg atcagtgccg acggacaggc cacgttgtgc ttctcgccca ccgcgcgatt
     7861 gagccagtag gccagggctg aattgaagaa cacctggatc aggatcggca ccgccagcat
     7921 ggcgatgatc aacggctgcc gcaggatctg ctcaccctgg aatgcaaaca gcagcaccag
     7981 tgtcgcgagc agtgcagcaa tcgaccacgg cccgagacgc gccagcagcg cgttgagtgc
     8041 cgccggcccg ccccgcgcga ggacataggc gcggatcagt tgcgcgatga tcaccggtac
     8101 cacgatgtat agcgccaccg aggtcagcaa ggtgtcccac ggcaccgtga tcgacgccac
     8161 gccaagcagc agcgcgacga tcggtgcgaa tgcgaagatc atgatcgtgt cgttcagcgc
     8221 cacctgcgac agcgtgaaat acggatcgcc gtgggtcaac cggctccata caaacaccat
     8281 ggccgtgcac ggcgcggcag ccagcaggat cagcccggcg atatagctgt cgatctggtc
     8341 cgccggcagc cacggcgcaa acagctggcg gatgaagatc cacgccagca gcgccattga
     8401 aaagggcttg accagccagt tgatgaagag cgtgacgccg atgccgcgca catgccgccg
     8461 cacctggccg agcgcggcga agtcgatccg cagcagcatc gggatgatca tcacccagat
     8521 cagcacgccg accgggagat tgacgcgcgc gatctccatc gtgccgatca cctgcaccgg
     8581 ccccggcaac agctggccga tcgcgatgcc ggcgacgatg cacagcgcga cccagaccgt
     8641 caggtagcgc tcgaacaacg acatcgcagc cgccggcgta ggcagcgcaa cggcctccgt
     8701 catgccgcgc tctgcgtcgc cccgctgcgg ccgatctcgt taaggcgctg ctgcagcgag
     8761 agcttgtcga gtttcgcgag cggcaggctc aggaaaagct cgatgcgccg ctgcaaggtg
     8821 gtgaacgcac ggaagaatgc cttgcgcttg tcctcgtcac cgccctcgat cgcagcagga
     8881 tcctcgacgc cccagtgtgc agtgaccggc tgcccgggcc acattgggca gacctcgccg
     8941 gccgcgttgt cgcaaaccgt gaagacgaag tccagcggtg gcgcgccggc tgcggcgaac
     9001 tcgtcccagc tcttgctgcg cagcccctcc gttggcaggc gactcttttg cagcagctcg
     9061 atcgcaaatg gattcacggt tccggttggc atgctgccgg cgctgtgagc gatgaatcgt
     9121 cctttggcca tcgcgttcat catcacttcg ctcatgatcg agcgagccga gttgcctgtg
     9181 cataggaaca gcacattcat gggttgttgg gacacgtcgc tctcctagct gcagcaggcc
     9241 gacgcggcct tggcacgcgc gctcgtaggc atcgccgcgg tggcatcgcc ggcgcagcag
     9301 ccggtggcct gggtcgtcac ttccgtatcc gaaccattga acgtcggaac cgatcccatt
     9361 gtgtggaagg tctcccacgc aatgccggtc gggtcggtga cccagtactt gttcgagcgc
     9421 gcatagcagc aggatgcttc cttctccgcc tgtgtggcca ggtcggcccg ctgcaactgg
     9481 gcattcaacg attcgagttc gccgtcggag tcggtctgca ggccgagatg attcaggccc
     9541 ggcttgccgc cgcgctgcga gatcgcgaag ttgacgcgcg ggtcttcgag catccacttg
     9601 gcgtagtcgc ccttgacgac ggccggctcg gtgccgaaca gcgtcgagta gaagcggatg
     9661 ctcgagtcga gatccgggac cgatacgtga acgtgaaagc gcttcatgcc ttgctccttt
     9721 ggggtttgcg ccggcctgcc gtggcgcgct tcgggggtgc gggcgaacag gcggcgaggg
     9781 gcgcgcacaa ggcgcccggg ccgcagtcgg caacgcagca gttatccgtc aaataggcga
     9841 gcaggtcatt catcgccgtg aagttcgccg catagaaaat gaagcgaccc tcgtggcgcg
     9901 cagtgaccag tcccgcgtgc gtcagttcct tcaaatggaa gctcaaggtt gctggcgcaa
     9961 tcgtcaactc ctcggcaatt tcacccgctg ccatgccgct cggcccctgc tcgaccagca
    10021 gccgaaagac gccgagacgg gtttcctgtg ccagcgcgcc gagagcacga acaaccgtta
    10081 ttgattccat ggttcgaata atatggaagc gttgcgggga aagtcaagtc cattttttca
    10141 gatgcactcg ttccctagcc ttgcgcttcg ccggaccggg gcggcggaag gtacacgccg
    10201 acctccggct tcagctctgt gctgaccgtt cgaagtcaat gtagtcgcgg gtcagctccg
    10261 ccagcgtgcg atagaaaggc gtctgcgcct gagcgatgac ggactcggca aactgcgcaa
    10321 gccagccagc gagatgctcc tttaacaacc gcctgcgcag cgctttcgca cccagttgtt
    10381 tgtccgtctg cccatcgcga cctgcctgcg cctcgcggaa cagcagtgca tacaggcact
    10441 cgagttcgac ggagacgtga tccggcaggt cgcgaaagtc ctcggctatt tcgaaccctg
    10501 cttcgcgata caactccatg acggctaccg tcgagtcctc catcacggtc tgcgttgtca
    10561 tcgacagcca caccgacgca tagggctgcg ccagcgccct cggaggaccg tggaaaagcc
    10621 gcacgtagtc gatcagcagc tcctgtcgat ccgcttcggc gtatgcactt tcgagtctct
    10681 gggcgaccgc gccgagcgca ggatcgatgc tactggctgc ctcccgtact gaagcgaaca
    10741 actgctcttc ggcaaactcc tcgcccggct cgtagtagct ggcggagagg agccgcgcaa
    10801 gatcggcgcg cgccgccagg agccggactt cgtcagtcag catgggaatc tccgaaaaaa
    10861 accggacgca gctgggatgc tgcgtccggc tcaggcttgg ttgcctatcg ctcagccgcg
    10921 cgc
//
LOCUS       DATIRT010000166         4625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1695309, whole genome shotgun sequence.
ACCESSION   DATIRT010000166 DATIRT010000000
VERSION     DATIRT010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1695309"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /gene="rplK"
                     /locus_tag="VLN25_03955"
     CDS             <1..418
                     /gene="rplK"
                     /locus_tag="VLN25_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HSQ09757.1"
                     /translation="VGFIKLQVPAGKANPSPPIGPALGQRGLNIMEFCKAFNAQTQGV
                     EPGLPIPVVITAFADKSFTFVMKTPPATVLIKKAAKIDKGSPKPHTDKVGKITRAQAE
                     DIAKAKMPDLTAAGLDAAVRTIAGSARSMGITVEGL"
     gene            419..1111
                     /gene="rplA"
                     /locus_tag="VLN25_03960"
     CDS             419..1111
                     /gene="rplA"
                     /locus_tag="VLN25_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HSQ09758.1"
                     /translation="MTISKRSKTIHAKVDPQKHYAIADALALVKECATAKFDESVDVA
                     VVLGIDAKKSDQTVRGSVVLPAGSGKTKRVAVFAQGAKADEAKAAGADIVGFEDLAAK
                     VKEGFMDFDVVIASPDAMRVVGQLGQILGPRGLMPNPKVGTVTPDVVQAVKNAKAGQV
                     QFRADKAGIVHTTIGRASFEVDRLKTNLSALVEALNKVKPASSKGIYLRKISVSSTMG
                     IGVRVDAASLNG"
     gene            1481..2011
                     /gene="rplJ"
                     /locus_tag="VLN25_03965"
     CDS             1481..2011
                     /gene="rplJ"
                     /locus_tag="VLN25_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HSQ09759.1"
                     /translation="MGLNRQEKAAKVEEVSALVASAQTIVVAEYRGLDVESATKLRKQ
                     ARSQGVQLRVLKNTLARRAVTGTPFAGLSDKLVGPLVYGFSADPVAAAKVLSSFAKDN
                     DKLVVKAGAMANFVMDEKGVKALATMPSREELLAKLMATMQAPIAQFVRTLNEVPSRL
                     VRTLAAVRDAKEATAA"
     gene            2060..2440
                     /gene="rplL"
                     /locus_tag="VLN25_03970"
     CDS             2060..2440
                     /gene="rplL"
                     /locus_tag="VLN25_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HSQ09760.1"
                     /translation="MALSNQEILDAIAAKSVLEISELIKLMEEKFGVSAAAAAVAVAA
                     PAAGAAAPAAEEQTEFNVILAEIGPNKVNVIKAVRELTGLGLKEAKDLVDGAPKPVKE
                     AVNKADAEAAKKKLEDAGAKVDVK"
     gene            2679..>4625
                     /gene="rpoB"
                     /locus_tag="VLN25_03975"
     CDS             2679..>4625
                     /gene="rpoB"
                     /locus_tag="VLN25_03975"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157779.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HSQ09761.1"
                     /translation="MPYSFTEKKRIRKSFAKRPSVLAVPFLLATQLESYMQFLQADTP
                     ASKRKTEGLQAAFSSIFPISSHNGMARLEFVSFNLGEPAFDVKECQQRGLTYASPLRA
                     KVRLVIMDREASKPTVKEVKEQEVYMGEIPLMTTTGSFVINGTERVIVSQLHRSPGVF
                     FEHDRGKTHSSGKLLFSARVIPYRGSWLDFEFDPKDILYFRVDRRRKMPATILLKAIG
                     LSNEQVLAHFFRSDTFQLAAEGAFMELVPERLRGEIARFDITDKAGKVVVAKDKRINA
                     KHIRDMEAAGMKRIAVTEDYLLGRVLANKVVDPETGEIVANANDEVTEEALKKLRVAN
                     VQEIRTIYTNDLDQGPFISNTLRIDETGDQLAARIAIYRMMRPGEPPTEDAVETLFRR
                     LFFDDETYDLSRVGRMKVNSRFARPEITGPMTLTNEDIIDTMKLLVELRNGRGEIDDI
                     DHLGNRRVRCVGELAENQFRAGLVRVERAVKERLGQAETENLMPHDLINSKPISASIR
                     EFFGSSQLSQFMDQTNPLSEITHKRRVSALGPGGLTRERAGFEVRDVHPTHYGRVCPI
                     ETPEGPNIGLINSMALYARLNEYGFLETPYRKVEKGHVTTEISYLSAIEEGKYVIAQA
                     NAPLDKTGKFMDELVSCRENGEFVL"
BASE COUNT          962 a         1428 c         1477 g          758 t
ORIGIN      
        1 cgtcggcttt atcaagctgc aggtgccggc aggaaaggcg aatccgtcgc ctccgatcgg
       61 ccccgcgctc ggccagcgcg gcctgaacat catggaattc tgcaaggcct tcaacgccca
      121 gacacaaggc gtggaaccgg gcctgccgat tccggtggtg atcacggcct ttgcggacaa
      181 gagcttcacc ttcgtgatga agacgccacc ggcaacggtg ctgatcaaga aggccgcgaa
      241 gatcgacaag ggttcgccca agccgcacac cgacaaggtt ggcaagatca cccgtgccca
      301 ggctgaggac atcgccaagg cgaagatgcc ggacctcacc gccgccgggc tcgatgccgc
      361 tgtgcgcacg attgccggca gcgcccggtc tatgggcatc acggtggagg gcctgtagat
      421 gacgatttcg aagcgctcca agacgatcca cgccaaggtc gatccgcaga agcactatgc
      481 gattgccgat gcgcttgctc tggtgaagga atgcgccacc gccaagttcg acgagtcagt
      541 cgacgtcgcg gtggtgctgg gcatcgacgc gaagaagtcg gatcagacgg tccgtggatc
      601 ggtcgtgctg ccggcgggca gcggcaagac caagcgcgtc gccgtgttcg cgcagggtgc
      661 caaggcggac gaggccaagg cagcgggcgc cgacatcgtc ggctttgaag acctcgcagc
      721 gaaggtcaag gaagggttca tggacttcga tgtcgtgatc gcttctccgg atgcgatgcg
      781 cgtcgtcggc cagttgggac agatccttgg accgcgcggc ctgatgccga accccaaggt
      841 tggcacggtg acgccggacg tcgtacaggc agtaaagaac gccaaggcag gccaggtgca
      901 gtttcgcgcc gacaaggcgg gcatcgtgca tacgacgatc ggtcgcgcct cgttcgaagt
      961 cgatcggctc aagaccaacc tgtcggccct ggtcgaagcg ttgaacaagg tcaagccggc
     1021 gtcgagcaag ggcatctacc tgcgcaagat ttcggtctcc tcgacgatgg gcatcggcgt
     1081 gcgcgtcgat gcggcatcgc tgaacgggta gttcgagagg caaaggattc gactcggctg
     1141 cgaccgggtc acaagggact ttgggctgcg cgctgcgtcg gaacatcgat acgggcgcgc
     1201 tgctgtcaaa gaccgctgga cgagaacgac accgatggcg cgagcgtgca acgaatgcgc
     1261 cccagggtct tctccttaat caccgtctgc tctgtctcgc atcgaaacgc tcggcgatga
     1321 caggtcggca gcaggagtcc agcgcagacg gccccgagga agacatggac agtgcgttgt
     1381 tcagcacttg agatgtcgct tcggacgccg ggtggtgcaa ccggtgccta gccagtgcgc
     1441 aagcacacat gcgaagcaca ttttgagtgg agccagtacc gtgggtctca atcggcagga
     1501 gaaagcggcc aaggtcgaag aggtcagtgc ccttgtggca tcggcccaga cgatcgtcgt
     1561 cgccgagtac cgtggtctgg atgtggaatc ggccaccaag ctgcgcaagc aagcgcgcag
     1621 ccagggtgtc caactgcgcg tgctgaagaa cacgctggca cgccgtgccg tcactggaac
     1681 cccgttcgcc ggactgtccg acaagttggt cgggccgctg gtgtatggat tttccgctga
     1741 cccggtcgcg gcagccaaag tgctctccag ctttgccaag gacaacgaca agctcgtcgt
     1801 caaagccggc gcgatggcca atttcgtcat ggacgaaaag ggcgtcaaag cgctggcgac
     1861 gatgccgagc cgcgaggaac tcctggcgaa gctgatggca acgatgcagg caccgatcgc
     1921 acagttcgtt cgcacgctga acgaagtgcc gtcgcgcctc gtccgcacgc tcgccgcagt
     1981 ccgcgatgcc aaggaagcaa cggcggcctg atccccatcc caaattccgg aactgaatca
     2041 acgcacagga gtcaatcaaa tggcactttc gaatcaagaa atcctcgacg cgatcgccgc
     2101 aaagtcggtg ctcgagatca gcgaactgat caagttgatg gaagagaagt tcggcgtctc
     2161 ggccgcagcg gccgcggtgg ccgtggccgc gccggcggcg ggcgctgctg cgccggccgc
     2221 tgaagagcag accgagttca acgtcatcct ggcggaaatc ggccccaaca aggtcaacgt
     2281 catcaaggca gtgcgtgagc tgacgggcct gggtctgaag gaagccaagg atctggtcga
     2341 cggcgcaccg aagcccgtaa aggaagccgt gaacaaggcc gatgccgaag ccgccaagaa
     2401 gaagctggaa gacgccggcg ccaaggtcga cgtgaagtaa acggcaaggg atgccggcgg
     2461 aggcagcttc gccggtgtcc cgaaccgaaa ggccgggagg cctttgacct gcagccatcg
     2521 gcttcagatc aaagtcttcc cctgaatgag cggacgcgca ggattcccct acgccgcccg
     2581 ggcgacagcc actgctgtgc ccgcggtggc gcttgtcgtc cctgcaaacg cccggtcagg
     2641 ccgcattttt tctctctccc gcgtcgaacg gagtgtccat gccgtactcg ttcactgaaa
     2701 agaagcgcat tcgcaagagc tttgccaaga ggccaagcgt tctggcggtg cctttcctgc
     2761 ttgcgaccca gctcgagtcg tacatgcaat tcctgcaggc ggatacgccc gccagcaagc
     2821 gcaagaccga aggcctgcag gcggcgttct cgtccatctt tccgatctcc agccacaacg
     2881 gcatggcccg gctcgaattc gtcagcttca atctgggcga gcccgcgttc gacgtgaagg
     2941 aatgccagca gcgcggactg acctacgcct cgccgctgcg cgccaaggtg cgactcgtga
     3001 tcatggaccg cgaggccagc aagccgacgg tcaaggaagt gaaggagcag gaggtttaca
     3061 tgggcgagat tccgctcatg acgaccaccg gttcgttcgt gatcaacggc acggagcgcg
     3121 taatcgtcag ccagctgcac cgctcgcccg gcgtgttctt cgagcacgac cgcggcaaga
     3181 cgcattcgtc gggcaagctg ctcttctctg cacgcgtgat cccgtaccgc ggttcgtggc
     3241 tcgacttcga gttcgatccg aaggacatcc tgtacttccg cgtcgaccgt cgccgcaaga
     3301 tgccggcaac gatcctgctt aaggcgattg gcctcagcaa cgagcaggtg ctggcgcact
     3361 ttttccgctc cgatacgttc cagctggctg ccgaaggcgc cttcatggaa ctcgtgccgg
     3421 agagactgcg tggcgagatc gcgcgcttcg acatcacgga caaggccggc aaggtcgtcg
     3481 tcgccaagga caagcgtatc aacgccaagc acatccgcga catggaagcg gcgggcatga
     3541 aacgcatcgc tgtcaccgag gactatctgc tcggccgcgt gctcgccaac aaagtcgtcg
     3601 accccgagac cggggagatc gttgccaatg cgaacgatga ggtgaccgag gaagcgctca
     3661 agaagcttcg cgtcgcgaac gtgcaggaga tccgcacgat ctacacgaac gacctggacc
     3721 agggaccgtt catctcgaac acgctgcgca tcgacgagac cggcgatcag ctggcggcgc
     3781 gcattgccat ctaccgcatg atgcgacccg gcgagccgcc gacggaagat gcggtcgaga
     3841 cgctgttccg gcgcctgttc ttcgacgacg agacctacga cctgtcacgg gtcggccgca
     3901 tgaaggtcaa ttcgcgcttc gcccgtccgg agatcacggg gccgatgacg ctgaccaacg
     3961 aggacatcat cgacacgatg aagctcctcg tcgagttgcg caatggccgc ggtgaaatcg
     4021 acgacatcga ccacctaggc aatcgccgtg tgcgctgcgt cggcgagctg gcggaaaacc
     4081 agttccgcgc gggcctcgtg cgcgtagagc gcgcggtcaa ggaacgcctc ggccaggccg
     4141 agaccgaaaa cctgatgccg catgacctga tcaattccaa gccgatcagc gcatcgattc
     4201 gcgagttctt cgggtcgtcc cagctgtcgc agttcatgga ccagaccaac ccgctgtcgg
     4261 agattacgca caagcggcgt gtgtccgccc tgggaccggg cggcctgaca cgcgaacgcg
     4321 ccggcttcga ggtgcgcgac gtgcacccga cccactacgg ccgcgtgtgc ccgatcgaga
     4381 cgcctgaagg cccgaacatc ggcctgatca actcgatggc actgtatgca cgcctgaacg
     4441 agtacggctt ccttgagacg ccgtatcgca aggtcgagaa aggccacgtc accaccgaga
     4501 tctcgtatct gtccgcgatc gaggaaggca agtacgtgat cgcgcaggcc aatgccccgc
     4561 tcgacaagac gggcaagttc atggacgagc tcgtgagctg ccgcgagaac ggtgaattcg
     4621 tgctg
//
LOCUS       DATIRT010000167         2057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1301750, whole genome shotgun sequence.
ACCESSION   DATIRT010000167 DATIRT010000000
VERSION     DATIRT010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301750"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="VLN25_03980"
     CDS             complement(<1..1116)
                     /locus_tag="VLN25_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HSQ09762.1"
                     /translation="MKIRKELWFGFIIMVLIIAGTAVMLLSADNLASGHLGLMMLALV
                     VVAIMLGFPTAFTLMGMGMIFTWLAYDRDITRTLDLIVQAAFKVMSNDVLISIPLFVF
                     MGYLVERANLIERLFRSLHLAMARVPGSLAVATIFTCAVFATATGIVGAVVTLMGLLA
                     LPQMLKAGYDVRVSAGSITAGGCLGILIPPSVLLIVYGATAGVSVVQLYAGAFFPGIM
                     LAGLYVVYVVVLAKIKPKLMPPLTTEQRFVPLPPMTERVSNAISNRALPALLGALRRA
                     RNIGVPTDYLVKQLVVALLPALLFTAVFALAWQINTAPGAAAQAAAAGGGLSEPAGTL
                     REPASGGGIQEPPGSSSGLKEPPGSVGLSEPPGSGSGV"
     gene            complement(1113..1745)
                     /locus_tag="VLN25_03985"
     CDS             complement(1113..1745)
                     /locus_tag="VLN25_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HSQ09763.1"
                     /translation="MQKLLLAVDRLSTWLGQVFAWMIILLTGMISWEVYSRYVLNTPH
                     DWALNLQIMMYGALFMMAGAYTLSKNGHVRGDVLYGFFQPRTQATIDLVLYVVFFLPG
                     IVALTYAGWIYANEALAIREKTFSATPLPLYPFKFIIPFAGAMLLLQGIVEIVRCIRC
                     LQDGNWPSREQDVEEVDVDKLKQMVHVKDEDIAALDKIVIAQELKQGTAP"
     gene            complement(1827..>2057)
                     /locus_tag="VLN25_03990"
     CDS             complement(1827..>2057)
                     /locus_tag="VLN25_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HSQ09764.1"
                     /translation="YIELKKMGVNFYKTPDAILRAQLASWDKIMEKKSAENALFKKVL
                     DSQKAFAQRASSWQNDYTVDFKMASNYYFGKK"
BASE COUNT          414 a          658 c          612 g          373 t
ORIGIN      
        1 cacgccgctg cctgatcccg gcggttccga aaggcctacg ctgccgggag gctctttcaa
       61 accgctcgaa gaacccggag gctcctggat tccaccgccc gaggcgggtt ccctgagcgt
      121 gcccgccggc tccgacaatc cgcctcccgc ggcggctgcc tgtgcggccg cgcccggtgc
      181 ggtgttgatc tgccatgcca gcgcgaagac ggcagtgaac agcagtgccg gcagcagtgc
      241 gaccacaagc tgcttgacca gatagtcggt cggcacaccg atgttgcgcg ccctcctgag
      301 cgcaccgaga agcgccggca gcgcgcggtt ggaaatcgcg ttcgacaccc tttccgtcat
      361 tggcggcagc gggacgaatc gctgctcggt ggtcaacggc ggcatcagct tcggcttgat
      421 cttcgccagc acgaccacgt acacgacata taggccggcc agcatgatgc cggggaagaa
      481 ggcaccggcg tacaactgca ccaccgaaac acccgcagtc gcaccataga caatcaatag
      541 gacgctcggc gggatcagga tgccgaggca acctccggcc gtgatcgatc ccgccgagac
      601 gcgcacgtcg tatccggcct tgagcatttg cggcagcgcc agcagtccca tcagggtgac
      661 aacggcgccg acgatgccgg tcgccgtcgc gaacactgcg caggtgaaaa tggtggcgac
      721 cgcaagcgag cccggcacgc gtgccatcgc caggtgcagg cttcggaaca gccgctcgat
      781 caggttcgcg cgctccacca ggtagcccat gaacacgaac agcggaatgg aaatcagcac
      841 gtcgttgctc atcaccttga acgccgcctg cactatcagg tccagcgtcc gcgtgatgtc
      901 gcggtcgtag gcgagccacg tgaagatcat ccccatgccc atcagcgtga atgctgtagg
      961 gaatcccagc atgatcgcca ccacgaccag cgccagcatc atcaaaccga ggtgaccgct
     1021 cgctagattg tcggccgaca gcagcatcac tgcggtgccc gcgatgatga gcaccatgat
     1081 gatgaatccg aaccagagtt ccttgcggat cttcatggcg cggttccctg cttcaactcc
     1141 tgcgcaatca cgatcttgtc gagggcggca atgtcctcgt ccttcacgtg caccatctgc
     1201 ttgagcttgt cgacgtccac ttcctcgacg tcctgctcgc gcgacggcca gttgccgtcc
     1261 tgcaggcagc ggatgcagcg cacgatctcc acgatgcctt gcagcagcag cattgctccg
     1321 gcgaagggga tgatgaactt gaacgggtag agaggcagcg gcgtggcgga aaatgtcttc
     1381 tcacggatcg ccagcgcctc gtttgcatag atccagcccg cgtaggtcag cgcgacgatt
     1441 cccggcagga agaacacgac atagaggacg aggtcgatcg tcgcctgtgt gcgcggctga
     1501 aagaagccgt acagcacatc tccgcgaacg tggccgtttt tcgagagcgt ataagcgccg
     1561 gccatcatga acagcgctcc gtacatcatg atctgcaggt tcagcgccca gtcgtgcggt
     1621 gtattcagca cgtagcggga atagacctcc caggagatca tcccggtcag caggatgatc
     1681 atccacgcga aaacctggcc cagccatgtc gacaaccggt ccactgccag cagaagtttc
     1741 tgcatcgccc ttccttttgc gggaagaaaa agggccaccg accgttgctg ctggcaggtg
     1801 gcccgtcgga cctcaaccgg cggtgactat ttcttgccga agtagtagtt ggaggccatc
     1861 ttgaagtcga ccgtgtagtc gttctgccac gagctggcac gctgcgcgaa ggccttctga
     1921 gagtcgagca ccttcttgaa cagcgcattt tccgcggact tcttctccat gatcttgtcc
     1981 cacgaggcga gctgcgcgcg caggatcgcg tcgggggtct tgtagaagtt cacgcccatt
     2041 ttcttcaact cgatgta
//
LOCUS       DATIRT010000168         4313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_908593, whole genome shotgun sequence.
ACCESSION   DATIRT010000168 DATIRT010000000
VERSION     DATIRT010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908593"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..888
                     /locus_tag="VLN25_03995"
                     /pseudo
     CDS             31..888
                     /locus_tag="VLN25_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701144.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            939..1502
                     /gene="ampD"
                     /locus_tag="VLN25_04000"
     CDS             939..1502
                     /gene="ampD"
                     /locus_tag="VLN25_04000"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HSQ09765.1"
                     /translation="MAPSVFSVGPDGWVAPARRHPSPNCDARPTGTAISLVVIHNISL
                     PPGCYGGVHVERLFTNGIEAGEHPILDRLRDAKVSAHFFIDRAGGSMQFVSCLERAWH
                     AGASSFKGRARCNDFSIGIELEGTDFEPFVDKQYVTLNALLAALAAAFPLEAIVGHSD
                     IALDRKTDPGPFFCWNRLDAPSHLLAR"
     gene            1878..>4313
                     /locus_tag="VLN25_04005"
     CDS             1878..>4313
                     /locus_tag="VLN25_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HSQ09766.1"
                     /translation="MQFQQSASPAVVGVAAHLSTVETGGGVSNVQATSYGDYKVIRRN
                     GAVVAFEPSKIAVAMTKAFIAVHGGQGAASAAIREQVEKLTSSVVTALMRRQPAGGTF
                     HIEDVQDQVELSLMRDGAHDVARSYVLYRERRAQERAATRQAAPAAAAPPLAMIEEGR
                     RVPLDMTRIEALVRSACEGLSENVAPDAILSETFKNLYDGVPLEEVYKSAILAARGLI
                     EKDPAYSYVTARLLLHTMRREALGEEVAQSAMAGRYADYFPKFIRYGIEAELLDDKLA
                     LFDQKKLARALKPERDLQFNYLGLQTLYDRYFLHHEGRRFELPQAFFMRVAMGLALNE
                     IDREARAIEFYEVLSTFDFMSSTPTLFNAGTRHSQLSSCYLSTVSDNLDGIYEAIKEN
                     ALLAKYAGGLGNDWTPVRALGSHIKGTNGKSQGVVPFLKVVNDTAVAVNQGGKRKGAV
                     CCYLETWHLDIEEFLELRKNTGDDRRRTHDMNTANWIPDLFMKRVVDGADWTLFSPSD
                     CPDLHDKTGRAFEESYLRYEDKVARGELKLFRKVPAQSLWRKMLSMLFETGHPWITFK
                     DACNLRSPQQHVGTIHSSNLCTEITLNTSDTEIAVCNLGSVNLPAHMKGDGTGGFELD
                     HGKLKKTIRTAMRMLDNVIDINYYAVPKARNSNVRHRPVGLGIMGFQDCLHMMRTPYA
                     SEGAVEFSDRSMEAVCYYAYWASTELAEERGRYSSFAGSLWDRGILPQDSLGLLKEQR
                     GGHVDVDMSASMEWDALRGRIRQFGMRNSNCVAIAPTATISNIIGVDACIEPTFQNVF
                     VKSNLSGEFTVINE"
BASE COUNT          786 a         1376 c         1381 g          770 t
ORIGIN      
        1 gccatccacg agtcgtaggc gcgccgcgcg cagccattcg tggcagtcaa ctgtggcgcc
       61 attccagaaa cgctgatgga ggccgagttc tttggctatc gcaaaggagc cttcaccggc
      121 gccgatcgcg atcgcgacgg attttttcag gccgctgctg gcggaacgct gttcctcgac
      181 gaggttgccg acctgccact ggcgatgcag gtcaagctgc tgcgtgcgat ccaggaacgg
      241 cgcgtccgca aggtcggatc accggccgaa gactcggtgg acgtgcgact ggtcagcgcg
      301 acccatcagg acctcgcggc gcttgtcacg agcggccgtt tccggcagga cctctattac
      361 cggctcaatg tcatcgagct cagagtgccg ccgctgcgcg accggcttga ggacgtcgag
      421 atgctggcca tcgccattct tcgtcgcatt gccgaccgca ctcaggggcc aatgacacgg
      481 ctgtcgccgg cggcgctggc ggcgctcaag cgataccgct ttccgggcaa cgtacgtgaa
      541 ctcgaaaaca tcctcgagcg cgccgtggcg ctggccggcg acgatgtgct gatggccgat
      601 gacctgctgc tcccgggaga ggtcgacctg gcgctggagg ccgagccggc gcccgccgaa
      661 gatgtggccg cgtcgccgga tgatgacctg ggtcagaccg cggcggcggt ctacgacctt
      721 gtcgatggca tcccgacctc gttgccggcg ttcctcgacg agaacgagcg cagcgccatt
      781 cgtgcggcgc tggcaaaaac acgctacaac cgcaccgcag ccgcgcagct gctcggaatc
      841 actttccgtc agttgcgcta tcgaatgcag cggctcgaca tcaagtgaga cgcggtcact
      901 cttcggggtt tgtcttcatc ggcgagcgct gataagggat ggcgccgtcg gtcttcagcg
      961 ttggtccgga tggctgggtc gccccggcgc gtcgccaccc atcgccgaac tgcgacgcgc
     1021 gccccaccgg cacggcgatc tcgctcgtcg tcatacacaa catcagtctt ccgccgggtt
     1081 gctatggcgg cgtccacgtc gagcgcctgt tcaccaacgg catcgaggcc ggcgagcacc
     1141 ccatcctcga tcgccttcgc gatgcgaaag tctcggccca tttcttcatt gatcgcgcgg
     1201 ggggaagcat gcagttcgtg tcctgtctcg agcgcgcctg gcacgccggc gcctcgtcgt
     1261 tcaagggccg ggctcgctgc aacgatttct cgatcggcat cgaactcgaa ggcacggact
     1321 tcgaaccgtt tgtcgacaag cagtacgtga cgctcaacgc gttgctcgcg gcgctcgccg
     1381 cggcgttccc actcgaggcc atcgtcggac acagcgacat tgcgctcgat cgcaagaccg
     1441 accccggtcc tttcttttgc tggaacaggc tcgacgctcc gtcgcatctg ctcgcacgct
     1501 gataccgact tgcagtcgta gttgattcgg cgcgttttcg cgcatccgta ctccctacgg
     1561 tgcgccgccg catcgacagc ggttgaactc ctgaaaaact tctcctatga tgcgcacaca
     1621 caaaaactag tgcctgcaag cacgttgacc acaacatctt gtggcaaggg tcgggtcgaa
     1681 gttgcccgac gaacagcagg cgcaggcttg gccgagcggc ggaaagcggg tcctcatgcg
     1741 cagcggcgtc atgccgggtg cggaggctga gcgaaaaaag gaaagagccc gggcggctat
     1801 agcttgtggt gccggcgcgc tggatgcgcg ggcatagcgg gtccgccgtg gggcacctag
     1861 gagcaatcgg aggtcgtatg cagtttcagc agtccgcctc cccggcagtg gtcggggtgg
     1921 ccgcacatct atctacggtc gagaccggcg gcggggtcag caacgttcag gcgacgtcgt
     1981 acggggacta caaggtcatc cgccgcaatg gcgccgtcgt cgcgttcgag ccatcgaaga
     2041 tcgcggtggc gatgaccaag gcgttcatcg cggtgcacgg cgggcagggt gctgcgtcag
     2101 ccgcgatccg cgagcaggtt gagaagctca cctccagcgt ggtcaccgct ctgatgcgcc
     2161 gccagccggc gggcggcacc tttcacatcg aagatgttca ggatcaggtc gaactctcgc
     2221 tgatgcgcga cggtgcgcac gatgtcgcgc gctcttacgt gctgtatcgc gagcggcgcg
     2281 ctcaggagcg agccgccacg cggcaggccg cgccggccgc ggccgctccg ccgctggcga
     2341 tgatcgaaga ggggcgtcgt gtcccgctcg acatgacgcg catcgaggcg ctggtgcggt
     2401 cggcgtgcga gggcttgtcc gagaacgtag ccccggacgc cattctttca gagaccttca
     2461 agaacctgta tgacggcgtt ccgctcgagg aggtctacaa gtccgccatc ctggctgcgc
     2521 gtgggctgat cgagaaggat ccggcctatt cctatgtcac tgcgcggctg ctgttgcaca
     2581 cgatgcggcg cgaagcgctc ggtgaagagg tcgcgcagtc ggcgatggcc gggcgctacg
     2641 ccgactattt cccgaagttc atccggtacg gcatcgaagc cgagctgctt gacgacaagc
     2701 tggcgctgtt cgaccagaag aaactcgcgc gggcactgaa gcccgagcgc gacctgcagt
     2761 tcaactacct cggccttcag acgctgtacg accgctactt cttgcaccac gaaggccgcc
     2821 gctttgaact gccgcaggcg ttcttcatgc gtgtcgccat gggcctggcg ctcaacgaaa
     2881 tcgaccgcga agcacgcgcg atcgagtttt acgaggtcct gtcgacgttc gacttcatga
     2941 gctcgacgcc gacactgttc aacgcgggga cgcgacattc gcagctgtcg tcgtgctatc
     3001 tgtcgaccgt ctcggacaac ctcgacggta tctacgaggc gatcaaggag aacgcgctgc
     3061 tggccaagta cgccggcggg ctgggcaacg actggactcc ggtgcgggcg ctcggcagcc
     3121 atatcaaggg caccaacggc aagagtcagg gcgtcgtgcc gttcctgaaa gtggtcaacg
     3181 acaccgcggt ggcggtcaac cagggtggca agcgcaaggg agcggtgtgc tgctacctgg
     3241 aaacctggca cctcgacatc gaggagttcc tggagttgcg caagaacacc ggcgacgacc
     3301 ggcggcgcac ccacgacatg aacaccgcca actggattcc cgacctgttc atgaagcgcg
     3361 tggtggatgg cgccgactgg acgctcttct caccgagtga ctgcccggac ctgcacgaca
     3421 agaccggcag ggccttcgag gagtcctacc tgcgctacga ggacaaggtg gcgcgcggcg
     3481 aactcaagct gttcaggaaa gtgcctgcac aaagcctgtg gcgcaagatg ctttcgatgc
     3541 tgttcgagac cgggcatcca tggatcacat tcaaggacgc ctgcaacctg cgctcgccgc
     3601 agcagcatgt cggcacgatc cacagctcca acctgtgcac cgagatcact ctcaacacgt
     3661 ccgataccga gattgccgtc tgcaacctgg gttcggtcaa cctgccggcg cacatgaagg
     3721 gtgacggcac cggcggattc gagctcgatc acggcaagtt gaagaagacc atccgtaccg
     3781 ccatgcggat gctcgacaac gtcatcgaca tcaactacta cgcggtgccg aaggcgcgca
     3841 actccaacgt gcgtcaccgc ccggtcggac tcggcatcat gggctttcag gactgcctgc
     3901 acatgatgcg gacgccctac gcgtccgaag gcgcagtcga gttctccgat cgcagcatgg
     3961 aggcagtctg ctactacgcc tactgggcgt cgacggagct ggccgaggag cgcgggcgct
     4021 attcctcgtt tgccgggtct ctttgggacc gcggcatcct gccgcaagac tcgctcggcc
     4081 tgctcaagga gcagcgcgga ggccatgtcg acgtcgacat gagtgcctcg atggagtggg
     4141 acgcattgcg cgggcgaatt cgtcagttcg gcatgcgcaa ttcgaactgc gtggctatcg
     4201 cgccgaccgc gacgatctca aacatcatcg gggtcgacgc ctgcatcgag cccaccttcc
     4261 agaacgtttt cgtgaagtcg aacctctcgg gggagttcac cgtcatcaat gag
//
LOCUS       DATIRT010000169         2769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1105583, whole genome shotgun sequence.
ACCESSION   DATIRT010000169 DATIRT010000000
VERSION     DATIRT010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105583"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VLN25_04010"
     CDS             <1..571
                     /locus_tag="VLN25_04010"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HSQ09767.1"
                     /translation="ARIDTIATRNQLDVAPLWRMKPWMLANVFALFEAAQAGYMPALS
                     VEAYLTRIAKQGNKPILELESIEQQFELFERASPPTQIAFLEDAVKAVETNAARREIN
                     RIAQAWETGDSAALERLMAEMRAQTSVGARFTVDTILLGRHPAMVKRIEALMAEGKLY
                     LIAVGSLHLAGPDGLVAMLRARGYTVTPL"
     gene            591..1274
                     /gene="pdeM"
                     /locus_tag="VLN25_04015"
     CDS             591..1274
                     /gene="pdeM"
                     /locus_tag="VLN25_04015"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HSQ09768.1"
                     /translation="MRKPLTRLMVELGGGTWELLPQRAAFWIERRWLVIADAHFGKAA
                     TFRARGVPVPQGTTTDNLSRLDRLIDDLRPSAVVFLGDLFHAREAHAPATLAALHRWR
                     EEHDGLDLVLVEGNHDLAAGAPPAGLRIRCMTEPWRVESIVFCHHPQFVAGATAMAGH
                     LHPAVRLSGRADDSVRLPCFWLRDGMTVLPAFGSFTGGACIARSPGERVIALADDRLF
                     EIPELRSAA"
     gene            complement(1314..2126)
                     /locus_tag="VLN25_04020"
     CDS             complement(1314..2126)
                     /locus_tag="VLN25_04020"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HSQ09769.1"
                     /translation="MSTLPTLFLSHGSPMHAIAAGRAGDTWSALGRRLPRPQAILVAS
                     AHWETELPMVATAKQPETIHDFGGFPPELFRIRYPAPGAVDAAQRALELLHAAGLPAS
                     ANGCHGLDHGAWVPLRRLYPEADVPVAQISIQPSLDAEHHLQVGAALAPLAHEGVLVL
                     GSGHLTHNLREWITDARRRGSMSIDEREPEPYVSEFRDWVDTTLRSNDRSALARWWQL
                     APHAARAHPTPEHFLPLMIAVGAAGAEPMVEHIDAGVDSGVLEMDAYLFWPR"
     gene            complement(2198..2761)
                     /gene="gstA"
                     /locus_tag="VLN25_04025"
     CDS             complement(2198..2761)
                     /gene="gstA"
                     /locus_tag="VLN25_04025"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HSQ09770.1"
                     /translation="MVARAAGLPLSLVRVDTKAKKTAAGDDYRAINGKAYVPMLELDD
                     GTRLTEGPAIVQYLADKAPEKRLAPANGTMERYKLQEWLNFITSEIHKQFSPLFDASM
                     PDETKAKFRDKLAGRFDWIVEQLGDQEYLTGANFTVADAYLFTVLNWTKWTGIDLARW
                     PLLQSYVARVAARPEVQAAMKEEGLLK"
BASE COUNT          443 a          905 c          938 g          483 t
ORIGIN      
        1 ggcgcgcatc gataccatcg cgacgcgcaa tcagctcgac gtcgcgccac tgtggcgcat
       61 gaagccatgg atgctcgcca atgtcttcgc gctgttcgaa gcggcgcagg ccggttacat
      121 gccggcactg tcggtcgagg cgtacctgac ccggattgcg aagcaaggca acaaacccat
      181 cctcgaactc gaaagcatcg agcagcagtt cgaacttttc gagcgagcat cccctcccac
      241 ccagatcgcg ttcctcgagg atgcggtcaa ggcggtcgag accaatgccg cgcgccgcga
      301 aattaaccgc attgcacagg cttgggaaac gggcgattcc gcggcgctgg agcgactgat
      361 ggccgagatg cgtgcgcaga cgagcgtcgg ggcgcgcttc accgtggaca cgatcctgct
      421 cgggcggcat ccggcgatgg tcaagcgcat cgaggcgttg atggctgaag ggaagctgta
      481 cctgatcgcg gtaggttctc tgcatcttgc cgggccggac ggcctggtcg cgatgctgcg
      541 tgcccgcggt tacaccgtga cgcccttgtg acggcgctgt gaccacttca gtgaggaagc
      601 cgctgacgcg gctgatggtg gaactcggcg gtggcacgtg ggagctgctt cctcagcgcg
      661 cggccttctg gatcgagcgc cgctggctcg tcatcgccga tgcgcacttc ggcaaggcgg
      721 ccacgttccg cgcgcgtggt gtgccggtgc cgcaagggac gaccacggac aatctgtccc
      781 gcctcgatcg gctgatcgat gacttacgcc cgtccgctgt ggtctttctg ggcgatctct
      841 ttcatgcgcg ggaagcgcac gcgcccgcca cgctcgcagc gcttcatcgc tggcgcgagg
      901 aacacgacgg actcgacctg gtgctggtcg aaggcaatca cgatctcgcg gcgggcgcgc
      961 cgccggccgg gcttcgcatt cgttgcatga cggagccctg gcgagtcgag tcgattgtgt
     1021 tctgccatca cccgcaattc gtcgcgggtg cgactgccat ggcgggccat ctgcacccgg
     1081 ccgtgcgcct gagtggccgc gccgatgaca gcgtgcgtct gccgtgcttc tggttgcgcg
     1141 acggcatgac ggtgcttccg gcttttggat cgttcaccgg cggcgcatgc attgcgcgct
     1201 caccgggtga acgcgtcatc gcgctcgccg acgatcggtt gttcgagatc ccggagctgc
     1261 gcagcgccgc atgagcggcg ccgtctgcgc cgcagcgtgc gtgcgccgac cgatcagcgc
     1321 ggccagaaca ggtaggcgtc catctcgagc acgccgctgt cgacgccggc atcaatgtgc
     1381 tcgaccatcg gctccgcccc ggcggcaccg accgcgatca tcagcggaag aaagtgttca
     1441 ggcgtcgggt gcgcccgcgc cgcgtgcgga gccagctgcc accatcgcgc cagcgccgac
     1501 cgatcgttcg atcgcagcgt cgtgtcaacc cagtcacgaa actcgctgac atacggctcg
     1561 ggctcgcgct cgtcgatcga catcgagccg cggcggcgcg catcggtgat ccactcgcgc
     1621 agattgtgtg tgaggtggcc cgaaccgagc acgagcacgc cttcatgcgc cagtggcgcg
     1681 agggcggcgc cgacctgcag gtgatgctcc gcatcgagcg aaggctgaat cgagatctgc
     1741 gcgaccggca catcggcctc ggggtacagg cgccgcagcg gcacccacgc gccgtgatcc
     1801 aggccgtggc aaccgttggc actcgccggc aggcccgcgg catgcagcag ctccagcgcg
     1861 cgctgcgccg cgtccacagc gccgggcgcg ggataacgga tgcgaaacag ctcgggcgga
     1921 aaaccgccga aatcgtggat cgtctcgggc tgcttcgccg tcgccaccat cggcagctcg
     1981 gtctcccaat gagccgaggc gaccaggatc gcctgcggtc ggggcaggcg tcgacccagt
     2041 gcactccagg tgtcgccggc gcgtccggcc gcgatggcat gcatcggcga gccgtgcgaa
     2101 agaaatagcg tcgggagcgt ggacatgggt ggcctcgttg atcagagccg gaggagtagg
     2161 cgtgccttcc cctctggaac ttgcttgcgg aaggacctta cttcagcaga ccttcttcct
     2221 tcatcgcggc ctgcacctcc ggccgtgcgg cgacacgcgc cacgtagctt tgcagcaagg
     2281 gccagcgtgc caggtcgatg ccggtccact tcgtccagtt gagcacagtg aacaggtacg
     2341 cgtcggcgac ggtgaagttc gcaccggtca ggtactcctg gtcaccgagc tgctccacga
     2401 tccagtcgaa gcggccggcg agcttgtcgc ggaactttgc cttggtttca tccggcatgc
     2461 tcgcatcgaa cagcggcgag aactgcttgt ggatctcgga ggtgatgaag ttgagccact
     2521 cctgcagttt gtaccgctcc atcgtgccgt tggcgggtgc gaggcgcttt tccggcgcct
     2581 tgtcggccag atactgaacg atggccggac cttcggtcag gcgcgtgccg tcgtcgagtt
     2641 caagcatagg cacgtaggcc ttgccgttga tggcgcggta gtcgtcgccg gcggccgttt
     2701 tcttggcctt ggtgtcgaca cgcaccagcg acagcggcag accggccgca cgcgcgacga
     2761 tgtgcggcg
//
LOCUS       DATIRT010000170         7310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1597074, whole genome shotgun sequence.
ACCESSION   DATIRT010000170 DATIRT010000000
VERSION     DATIRT010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7310
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597074"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VLN25_04030"
     CDS             <1..802
                     /locus_tag="VLN25_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HSQ09771.1"
                     /translation="PGLPQVACFDTAFHRTLPLAEKMLPLPHAMWERGIRRYGFHGLS
                     YEYMSIALPERHGLHAEGRTIVAHLGSGASLCAMAGLQSVATTMGFSALDGLMMGTRC
                     GAVDPGVLLHLMATEKLSVEQLTNLLYHESGLLGVSGFSSSPKALLDAEVVNPRARAA
                     LALYLRRIVREIGALTAVLGGLDMLAFTGGVGEHSAILRWRICAALEWLGVHIDRDAN
                     SADAPTISARRSRVHVAVEPTNEEWIAATHARRLLRPTSPVDQGYAVA"
     gene            833..1447
                     /locus_tag="VLN25_04035"
     CDS             833..1447
                     /locus_tag="VLN25_04035"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSQ09772.1"
                     /translation="MNLKSLLLGRDRIAIRSIRPDDRERLVRAFHALDGQSIYLRFFF
                     HKRALSDKDLRQITECDGTRVVALVATVETGAQEAIVGLGQYARQGTTAHIAFAVEED
                     FQGRGIATRLLRRLVRIGRDQGISHFEADVLPENLPMLQVFRRSGLPLEESEEDGAVH
                     LTLVIGDANRQAEAPNAERYRPNGDRLLTTSAQAPTTVTTEPRC"
     gene            1692..4049
                     /locus_tag="VLN25_04040"
     CDS             1692..4049
                     /locus_tag="VLN25_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011356226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HSQ09773.1"
                     /translation="MNTPARQLSHSHDVAAKVTQVVQSRTTLARERFTERVKGACEQQ
                     VAAVMENPLSAWEIWTSGAQYAVDFAQRSILMLDTLRQRGNNFVEHERQGLPPVLRFD
                     YEIVMDGRSFKRPVNYALVRIVPPKGVTIDPKRRPFVIIDPRGGHGPGIGGFKDDSQV
                     GVALQEGHPVYFVIFFPHPEPGQTLLDVCNAERDFVHRVRKLHPDAPKPGIVGNCQAG
                     WAAMMLASAHPDDTGPVVIVGAPMSYWGGAWRENEGDNPMRYAGGLLGGTWLASLTSD
                     LGAGIFDGAWLVQNFENLNPANTFWDKYYKVFADIDTEAARFLEFERWWGGYYLLNGE
                     EIEWITQNLFVGNKLWSGTMKGGPAGALDLRQIKVPIVMFASMGDNITPPQQAFNWVA
                     DVYGSTEEIKARGQVIVGLMQEEVGHLGIFVSGKIARKEGTQIASVLKSIEALPPGLY
                     GMQIAEKKDAGGKAAYEVNLVELRLEDVVKSLNRFKRVDEKPFEAAKVVSEFNQRAYE
                     LFGRPLVKALANEYGAKLSREFHPLRYQRWAFSDLNPFLGWLAPAAQAVKAQRQALAS
                     DHPARQIEATASEMLSASVDCYRALRDTVAEATFFQIYGNLFEFYLGEGEAGDPETVA
                     PALDARELPFVKEVLASIDKGGYPEALARVGFLMAHRDEPLPLARVQLASDLLQDYQD
                     LLPDLPRDEVRRIGGEQEIIARYELDKAIETLPVLLAKRKDRDRLLSLLERVLRDKRV
                     QRIQPSPEQTEMLARIRRVLGQPSHRPAPVPARRRIKREVAAEAG"
     gene            4053..4376
                     /locus_tag="VLN25_04045"
     CDS             4053..4376
                     /locus_tag="VLN25_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09774.1"
                     /translation="MAAQTHDLPLAFWESNLEELDGEIARLAMLCDISIFEPGAIARV
                     LQSDASVCKSENPLAFAKLRALLMMHLAIREKSVDVFGKAATVRVEDYVIDRLKQRFP
                     HLKGE"
     gene            4426..4872
                     /locus_tag="VLN25_04050"
     CDS             4426..4872
                     /locus_tag="VLN25_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09775.1"
                     /translation="MDATLVGAMAGVLGSLVGGSATVATAWLTQTTASRRELVQMEFR
                     KREALYGEFIAECSKLLMDAMAHTLDKPETLLNAYALLNRIRLSASPPVLAEAEHLLR
                     RITEQYFSSNLTVEEMREIARSEGADPLQPFGEACRVEFASISARL"
     gene            5273..6289
                     /locus_tag="VLN25_04055"
     CDS             5273..6289
                     /locus_tag="VLN25_04055"
                     /inference="COORDINATES: protein motif:HMM:NF021497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HSQ09776.1"
                     /translation="MKTSLWMVLVALLLSSAVDCAAQSTAPYRGALTVTLENDVGSSD
                     NNYTNGLGLTWVSNAINTYDEGSFVRRWGEFWSFLPFVGNKGSRTYVSWSLAQEMHTP
                     DDITNPNPPLDDQPYAGVLYLDSVIYSKKERWAHAWQLRVGVVGPASQAENVQKWLHR
                     VIDSDEPMGWDTQLPDEPIINVGYTGTYLLAQGELGRSASWRVIPVAYAGLGNYFTGV
                     GLGLYGEVGWNLVDAFGGTALRLGFNAASTVGVGPVNGWSVSLSGGVVGFGVAHYLPL
                     DGTVFRDSRSVGSEPFIGMATLGIAVRHRGFTFFIGQTFFSKTFETERQRPEFGTLSL
                     SWIY"
     gene            6589..>7310
                     /locus_tag="VLN25_04060"
     CDS             6589..>7310
                     /locus_tag="VLN25_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSQ09777.1"
                     /translation="MPLLAYITRLPRLTGLKPMRRAAASAGALVAPLAASLAIGLAGC
                     STPVLKSAVDVPAHFAAGASTVDTDPDVAWWESYADPALTELIRRASRENRDVKIAAA
                     RVRAARAGATISRSWLFPSISAVGTGLHANTGYDAVKRQALPDADIWGAGVGVSWEID
                     LSGRLRAGATAAEADALAVEHGARGVRLLVLTDIATSYFTLVGASRQLETVRAISTAQ
                     DETLRLVTARHRAGLATPFDVER"
BASE COUNT         1328 a         2339 c         2332 g         1311 t
ORIGIN      
        1 gccagggttg ccgcaagtgg cgtgcttcga taccgcattc caccgtactc tgcccctggc
       61 ggagaagatg ctgccgttac cccatgcgat gtgggagcgc ggcatccggc gctacgggtt
      121 ccatgggctg tcttacgaat acatgtcgat cgccctgcct gaacgccatg gcttgcacgc
      181 agagggacgc acgatcgtcg cccatcttgg cagcggcgcc agcctgtgcg cgatggctgg
      241 cctgcaaagc gtggccacga cgatgggctt ttccgcgctc gacggtctga tgatggggac
      301 gcggtgcggg gcggtcgacc ccggcgtcct gctgcacttg atggcaaccg agaagctcag
      361 cgtggagcag ctcaccaatc tcctgtatca cgagtcgggg ctgctcggcg tctccggctt
      421 ctcctccagc ccgaaggccc tgctggacgc cgaagtggtc aatccgcggg cgcgggcagc
      481 gctggcactg tacttgcgac gcatcgtgcg ggagatcggc gcgctgaccg ctgtgctggg
      541 cgggctcgac atgctggcct tcaccggcgg cgtgggtgag cacagcgcaa tactgcgctg
      601 gcggatctgc gcggcactcg agtggctggg cgtgcacatc gaccgtgatg ccaacagcgc
      661 cgacgctccg accatctcgg cccggcgcag ccgggtccat gtcgccgtcg aaccaaccaa
      721 cgaggaatgg atcgcggcta cccacgcccg gcgcctgctg cgtcccacct cgccggtgga
      781 ccagggctac gccgtcgcct gacgcgaacg tgcccggaag gagagaatgc tcatgaacct
      841 aaagtcgctg ctgctcggcc gcgatcgcat cgcgattcgc tccatccgac ccgacgaccg
      901 cgaacgtctg gtgcgggcct ttcacgcact ggacggccaa tcgatctacc tgcgcttttt
      961 cttccacaag cgggcgttga gcgacaagga cctacggcag atcactgaat gtgacggcac
     1021 gcgcgtcgta gctctggttg ccacggtcga gaccggcgct caggaagcca tcgtcggact
     1081 gggtcagtac gcgaggcaag gcacgactgc gcatatcgcg ttcgcagtcg aagaggactt
     1141 ccagggacgc ggcatcgcca cccgtctgct gcggcgcctg gtgcgtatcg gtcgcgacca
     1201 gggcatctcc catttcgagg ccgacgtgct ccccgagaac ctgccgatgc tgcaggtgtt
     1261 caggcgcagc ggcctgccac tggaggaaag cgaagaggac ggcgcggttc acctgacgct
     1321 ggtgatcggt gatgccaatc ggcaggcaga agcgccgaac gccgagcgat acagaccgaa
     1381 cggcgaccgt ttgctcacca cttccgcgca ggccccgact accgttacta ctgaaccgcg
     1441 ttgctgattg acactgcgat gactctcaaa tccgaatcca cttatccaag ccggcgcact
     1501 tacgtgctga agttgcgtag cgacgccgga ccgaacgccc ttgcgggacg cctcgaaaat
     1561 cttgtgaccg gaggacagcg tgaattcgta tccggccagg cacttctcga gtcgatagcc
     1621 aacgatcttc agaccagcac gtccgagcgg tccgccgaag cggcgacggg cgatgaacaa
     1681 aggaagacct catgaacact ccagccagac aactgtccca cagccacgat gtggcagcta
     1741 aagtcacaca ggtcgtgcag tcgcgcacaa cgctggcgcg ggagcgtttc actgagcgcg
     1801 tgaagggagc gtgcgagcag caagtcgccg cggtgatgga gaatccgctg tccgcctggg
     1861 agatctggac cagcggggcg cagtatgcgg ttgacttcgc gcagcgttcg attctgatgc
     1921 tggacaccct gcgtcagcgc ggaaacaact ttgtcgaaca cgaacggcag ggactgccgc
     1981 cggtgctgcg cttcgactac gagatcgtga tggacgggcg ctcgttcaag cggccggtca
     2041 actatgcgct ggtgcgcatt gttccgccga agggggtgac gatcgatccc aagcgccgcc
     2101 ccttcgtgat cattgacccg cgcggcggcc atggccccgg catcggcggc ttcaaggacg
     2161 actcgcaggt cggcgtagcg ctgcaggaag gtcaccccgt gtacttcgtg atcttcttcc
     2221 cccatcccga gccgggacaa acgctgctcg acgtgtgcaa cgccgagcgc gacttcgtgc
     2281 atcgcgtgcg taagctccac cccgacgccc cgaaacccgg aatcgtcggc aactgccagg
     2341 ctggctgggc cgcgatgatg ctcgcgtccg ctcatccaga cgacaccggg ccggtggtaa
     2401 tcgtcggcgc accgatgtcc tactggggcg gcgcgtggag ggaaaacgag ggcgacaacc
     2461 cgatgcgcta cgcgggcggg cttctcggcg gcacctggct tgcatcgctg acctccgacc
     2521 tgggcgcggg catattcgac ggcgcctggc tggtgcagaa cttcgagaac ctgaatccgg
     2581 ccaacacgtt ctgggacaag tactacaagg tattcgccga catcgacact gaggcagcgc
     2641 gctttctcga gttcgagcgc tggtggggcg gctattacct gctcaacggc gaggagatcg
     2701 agtggatcac acagaatctt ttcgtcggca acaagctgtg gtcggggacg atgaaaggcg
     2761 gtccggccgg cgcacttgac ctgcgccaga tcaaggtacc gatcgtgatg ttcgcgtcga
     2821 tgggcgacaa catcaccccg ccgcagcagg cgttcaactg ggtcgccgac gtgtacggca
     2881 gcaccgagga gatcaaggcc cgcggccagg tgatcgttgg cctgatgcag gaggaagtcg
     2941 gccacctggg catcttcgtt tccggcaaga ttgccaggaa ggaaggaacg cagatcgctt
     3001 cggtgctcaa gtcgatcgag gcgctgcccc cgggcctgta cgggatgcag atcgccgaaa
     3061 agaaggacgc cgggggcaag gccgcctacg aggtcaacct cgttgagttg cgtctggaag
     3121 acgtggtcaa gagccttaac cgcttcaaac gcgtcgacga gaagcccttc gaggcggcca
     3181 aggtcgtctc ggaattcaat cagcgcgcct atgaactctt tgggcggccc ctggtgaagg
     3241 cactcgccaa cgagtacggc gccaagctgt cgcgcgagtt tcacccgctg cgctatcagc
     3301 gctgggcatt ttccgacctc aacccgttcc tgggttggct ggcgccggca gcgcaggcag
     3361 tgaaggcgca gcgtcaggcg ctggcgtccg accatccggc gcgccagatc gaagcgacgg
     3421 cgtcggagat gctgagcgcc tcggtcgatt gttatcgggc gctgcgtgac acagttgccg
     3481 aagcgacctt cttccagatc tacggcaacc tcttcgaatt ctatctgggc gaaggagagg
     3541 ccggcgatcc tgaaacggta gcaccggcgt tggacgcgcg cgagcttcca ttcgtcaagg
     3601 aagtgctggc ctcgatcgac aagggcggct accccgaggc gctggctcgc gtcggcttcc
     3661 tgatggcaca ccgggacgag ccgctgccgc tcgccagagt gcagctggcc agcgatctgc
     3721 tgcaggacta tcaggacctg ttgccggatc tgccccgcga cgaagtgcgg cgcatcggtg
     3781 gcgagcagga gatcatcgcc cgctacgagc tggacaaggc gatcgagacg ctgcccgtgc
     3841 tgttggccaa acgcaaggac cgcgatcgcc tcctctcgct gctcgagcgc gtgctccggg
     3901 acaagcgggt gcaacgcatc cagccttctc ccgagcaaac cgaaatgctc gcgcgcatcc
     3961 gtcgcgtgct tggccagcca agccaccggc cggcgccggt tccggcgcgc cgacggatca
     4021 agcgcgaagt tgcagcagaa gcaggatgat ccatggccgc gcaaacgcat gaccttcctc
     4081 tcgcgttctg ggagagcaac ctcgaagagc ttgatggcga aattgcgcgg ctggcgatgc
     4141 tgtgcgacat cagcatcttt gaacccggcg ccattgcgcg cgtgctgcag agtgacgcct
     4201 cggtgtgcaa aagcgagaat cccctcgcct tcgccaagct gcgcgcgctg ctgatgatgc
     4261 acctcgcgat acgcgagaag tcggtcgacg tcttcgggaa ggcggcgacg gtccgcgtgg
     4321 aggactatgt catcgaccgg ctcaagcaac gcttccctca cctgaaaggc gagtagtcgc
     4381 acgccggagt cctacggttc cgtcgacaaa gctcaaggct aagcaatgga tgcaaccctg
     4441 gtaggcgcta tggcgggagt gctgggatcg ctggtgggcg gctcggcaac cgtcgccact
     4501 gcgtggctca cgcagacaac cgccagcagg cgtgaactgg ttcaaatgga gttccgcaag
     4561 cgggaagcgc tgtacggcga gtttatcgcc gagtgcagca aacttctcat ggacgcgatg
     4621 gcccataccc tggacaagcc ggaaaccttg ttgaacgcct acgccctgct caaccgcatc
     4681 cggctatctg cctccccgcc ggtcctggcc gaagccgagc atttgttgag acgcatcacc
     4741 gagcagtatt tttccagcaa cctgaccgtt gaggagatgc gcgaaatcgc gcgttccgaa
     4801 ggggccgacc cgcttcagcc cttcggagag gcgtgccgcg tggagttcgc gtcgatcagc
     4861 gccaggttgt aggtcgctat ttccgacaga atcgctctcg gcgggggtcg tctgctcacc
     4921 actcgattgc tctgcaacct catctaactg cgaccgaaag ccgacgcttc gctgaccact
     4981 ttttgctagc gcgcggttac tctgacttca catcgagatc catttgatgt cagcgagcga
     5041 tgccgccaag tgctccccct gccaagaggc ctccgcgggc cggccgcgag ccgtttcgcc
     5101 aattcgaatg gccagcggga accaaatgtc gcgctcgcga tgtcacttgc gaccggtcgg
     5161 gccggctgca cccgctgatc gccaggccgc aaggcgcggc gctcctgctc atcggcagcg
     5221 tgctgttcgt catttcactt gtgcgctttt ggaagatgca gggggcaatg gcatgaaaac
     5281 gagcctgtgg atggtcctgg tcgcgttgct gctatccagc gcggtcgact gcgccgcgca
     5341 gtcaaccgcg ccctatcgtg gcgcattgac ggtgacgctc gaaaacgacg tagggtcctc
     5401 cgacaacaac tacaccaacg gcctcggcct gacttgggtc tcgaacgcca tcaataccta
     5461 cgacgagggg agcttcgtcc gccggtgggg tgagttctgg tcgttcctcc cgttcgttgg
     5521 gaacaaaggt tcccgcacgt atgtgtcctg gtccctcgct caggagatgc atacgccaga
     5581 cgacattacg aacccgaacc cgccgctgga cgaccagccg tatgcgggcg tgctgtacct
     5641 cgacagcgtc atctattcga aaaaggaacg gtgggctcac gcgtggcagc tccgcgttgg
     5701 tgtcgtcggt cccgcgtcgc aggctgaaaa cgtacagaag tggctccacc gagtgatcga
     5761 ctcggacgag ccgatgggct gggacacgca gcttccggac gagcccatca tcaacgtcgg
     5821 ttacaccggc acgtacctgc tggcccaggg ggaactgggc agatcggcct cgtggagagt
     5881 cattcccgtg gcctacgcgg gtctcggcaa ctacttcacc ggtgtcggac tcgggctgta
     5941 cggcgaggtc ggctggaacc tcgtcgatgc atttggcgga acagcgctgc gtctggggtt
     6001 caatgcggcg tcgacggtcg gtgtcgggcc agttaacgga tggtctgtgt cgctgtccgg
     6061 cggtgttgtg ggcttcggcg tggcccacta ccttccactc gacggtacgg tcttcaggga
     6121 cagccgatca gtcggctccg agcccttcat cggcatggcc accctcggca tcgccgtgcg
     6181 acatcgcggg ttcaccttct tcatcggcca gacattcttc agcaaaacgt tcgaaaccga
     6241 gcggcagcgc cccgagttcg gcactttgag cctgtcctgg atttactgaa aattgtcgat
     6301 ggtgtcgccc cggcgtgggt taccccgggg atcctcagca agcgcgaact gatccagacg
     6361 aagtcccgcg agcggtaggt tctggacggc ctgttcatcg tgctctacgc cggtcgatcc
     6421 gctctaaggt ggtgtcattc atgcgcagcc ccgtacgagg ctgctcgaca cccacactcc
     6481 cccgtcggaa ggtgctgacc aaatcgcgac caaacgtcga cgcctcactg accacttcct
     6541 gctgagcctt cactactgtt cgctcactga accaaacgtt gacgacccat gccgttactc
     6601 gcttacatca cccgactgcc tcggctgaca ggcctgaagc cgatgcgccg agccgcggcc
     6661 tctgcgggcg cgttggtggc cccgctcgcc gcgtcccttg cgatcggtct ggccgggtgt
     6721 tccacacccg ttctaaagtc cgccgttgac gtgccggcgc acttcgcagc gggtgcctcc
     6781 accgtcgata ccgatcccga cgtcgcgtgg tgggaaagct acgcagaccc agcgctaacc
     6841 gaactgattc gccgcgcatc gcgcgagaac cgggacgtga agatcgccgc tgcgcgcgtg
     6901 cgcgctgcgc gagccggcgc gacgatcagc cgttcctggc tctttcccag catcagtgcc
     6961 gtcgggacgg gacttcacgc caatacaggc tacgacgcag tcaaacggca ggcgctaccg
     7021 gatgccgaca tctggggcgc tggcgttggc gtttcgtggg agattgatct ttccggccgc
     7081 ctgcgagctg gcgctacggc ggccgaggcc gatgcgctcg ccgtcgagca tggcgcgcgc
     7141 ggcgtgcgcc tgctggtact gacagacatc gcgacgagct acttcacact ggtcggtgca
     7201 tcgcggcagc tcgaaaccgt ccgagctatc tcgacggcgc aggacgaaac gctgcgtctg
     7261 gtcacggcac gccatcgcgc cggccttgcg acgccgttcg acgtcgagcg
//
LOCUS       DATIRT010000171         6308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_121829, whole genome shotgun sequence.
ACCESSION   DATIRT010000171 DATIRT010000000
VERSION     DATIRT010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6308
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121829"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..179
                     /locus_tag="VLN25_04065"
     CDS             <1..179
                     /locus_tag="VLN25_04065"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HSQ09778.1"
                     /translation="PLNRVIKCWTEGVQKMKVGGKAKLTCPASVAYGERGTPGGPIPP
                     NATLIFEVELLGIK"
     gene            complement(580..1089)
                     /gene="mreD"
                     /locus_tag="VLN25_04070"
     CDS             complement(580..1089)
                     /gene="mreD"
                     /locus_tag="VLN25_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HSQ09779.1"
                     /translation="MPREYLLRPANPLFIWFSLIVALLVNLMPWGRTPFAPDVLALAL
                     VFWNVHQPRRVGMGAAFVFGLLMDVHQGALFGQHALAYTLVSYGAITLHRRIQWFPLG
                     AQAIHVLPLLLIAQLASLAIRMWVGGEWPGWIYFAQSLVGAILWPLIAWLLLAPQRRP
                     IDRDETRPI"
     gene            complement(1176..2078)
                     /gene="mreC"
                     /locus_tag="VLN25_04075"
     CDS             complement(1176..2078)
                     /gene="mreC"
                     /locus_tag="VLN25_04075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HSQ09780.1"
                     /translation="MEYGPPPLFNQGVSARARLAFFSFLAIALIVIDSRIKALETVRV
                     GVGVVLYPVQQVLLFPGRMADAVGDYFTSVTTLQRENEQLKRRQVESAQSLLQSQQVG
                     AENERLRKLLGARERTGHGAIVGTVLYESRDRFSHKIVLHVGTVDGVRAGNPVIDDVG
                     VVGQVTRVFSNTAEVTLLTDKDQSIPIQIVRNGLRGIAFGGADPGTLDLRFMAANADV
                     ENGDVAVTSGLDGIYPAGLPVGKVGIVERAAKDQFARIVLTPSAGVENHTHLLVLLVE
                     AAQRPAPPPPVQPEAVTRRGTGKR"
     gene            complement(2208..3251)
                     /locus_tag="VLN25_04080"
     CDS             complement(2208..3251)
                     /locus_tag="VLN25_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HSQ09781.1"
                     /translation="MFGFLRSYFSNDLAIDLGTANTLIYVRGKGIVLDEPSVVAIRQE
                     GGPNGKRTIQAVGKEAKQMLGKVPGNIEAIRPMKDGVIADFTVTEQMLKQFIKMVHPS
                     SLFAPNPRIIICVPCGSTQVERRAIKESAEAAGASQVFLIEEPMAAAIGAGLPVSEAS
                     GSMVVDIGGGTTEVGVISLGGMVYKGSVRVGGDKFDEAIINYIRRNYGMLIGEPTAES
                     IKKNIGSAFPGSEVKEIEVKGRNLSEGIPRSFTISSNEILEALTDPLNQIVSSVKIAL
                     EQTPPELGADIADRGMMLTGGGALLRDLDRLLMEETGLPVVVAEEPLTCVVRGCGIAL
                     ERMDKLGTIFATG"
     gene            3454..3741
                     /gene="gatC"
                     /locus_tag="VLN25_04085"
     CDS             3454..3741
                     /gene="gatC"
                     /locus_tag="VLN25_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011353557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HSQ09782.1"
                     /translation="MALDLAQIRRIADLARIDVTDTEAQRVLDQLNDIFRMIERMQAV
                     DTDALEPMAHPLGGAQRLREDEVTVGDERTANLANAPEQQDGLFLVPRVIE"
     gene            3845..5323
                     /gene="gatA"
                     /locus_tag="VLN25_04090"
     CDS             3845..5323
                     /gene="gatA"
                     /locus_tag="VLN25_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HSQ09783.1"
                     /translation="MSALDLGQATLADLSRLLAAREVSSVELARAYLARMEAQRSLNA
                     FLDVRPEVTLAQAAAADARIARGEATPLTGLPIAHKDIFVTKEFASTASSRILKGYMS
                     PFDATVVDNLSRAGMVTLGKLNCDEFAMGSSNENSAYGNVLNPWDHGAVPGGSSGGSA
                     AAVAARIAPVATATDTGGSIREPAAFSGITGTKPTYGRPSRWGMIAFASSLDTAGLMT
                     LSAEDAALVFNAMLGFDPKDSTSLDRPTEDYTRSIGASVKGMRIGVPKEFFGTGLQPD
                     VEAAVRAALVEYEKLGARLVPISLPNAELGIPVYYVIAPAEASSNLARFDGVRYGHRA
                     AKYTDLKDMIRKSRAEGFGDEPKRRILIGTYVLSHGYYDAYFIKAMKVRRLIANEFQQ
                     AFARCDVIAGPVTTSVAFGFGEKAADPVAMYMADLYTVPGSLAGVPSMSIPCGFGTGP
                     VNSRRPVGLQLIANYFEEAKLLGVAHAYQSATDWHRRVPAGA"
     gene            5332..>6308
                     /locus_tag="VLN25_04095"
     CDS             5332..>6308
                     /locus_tag="VLN25_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09784.1"
                     /translation="MSQRLLLVNQQLVDAARDPLVRARHLQDRVVLLARLGRVEQARA
                     LLESALRETPADAPAALRLRFDYASAIRLYFAKRFDDARTAMLDVAERARHADGDMAL
                     VGECESALALFMQREGDVRSAASYARAVLANRAATPESRYRAFLALGSLHQDAYDYEE
                     AGRLYREAEGLVRQLDDEIAMASWLKRSALTQAAHARQAAALGELDARTLANAVDALK
                     KSIAFAKGLAEGPEAMLDHLLLAEMHVLQRRYTDALTLYDAHLPSADGEGFLHEVTAA
                     MADSAHCCIELGRVDDGYAQLLSALRRVDESTPSDIRAIVHANMAAALK"
BASE COUNT         1131 a         2101 c         2038 g         1038 t
ORIGIN      
        1 ttccgctcaa tcgcgtgatc aagtgctgga ccgaaggcgt gcagaaaatg aaggtcggcg
       61 gcaaggccaa gctcacctgc cctgccagcg tcgcctacgg cgaacgcggc acgcccggcg
      121 gaccgatccc gccgaacgcg accttgatct tcgaggtcga gctgctggga atcaagtgac
      181 attcgccgct cacttacgcg cccttcgggc gcacgtgagc tcgcggcggg agcgcgcgct
      241 ctgcgcgcac tttgattcga gtcggctgcg ctccggagcg gccgcgcgct ccgccgactt
      301 ccgattgcac gacactctcc ctaaccctct cgccgggcga gagggaatcg tgctcccctc
      361 cctctccctc ccctccagga ggggagggca atgacgaaac agccgccggc tcggtgcgtc
      421 gcggagtggg cgcgtgccct tgtccttcgg cggagcgcgc ggccgctccg aagcgcagcc
      481 gacttatcag tgtcgagcgg ttcgcgcgca gcgcgaaacg tacccggagc gagtgcacgt
      541 gcgatcggcg cagccgatcg cgtacacgag cgacgattgt taaatcggcc tcgtttcgtc
      601 cctgtcgatc gggcgtcgct gaggcgcgag cagcagccag gcgatgagcg gccagaggat
      661 cgcgccgacc aggctctgtg caaagtagat ccacccgggc cactcgccgc cgacccacat
      721 gcggatggcg agcgaagcta gctgcgcgat cagaaggagc ggcagcacgt ggatggcctg
      781 cgcaccaagc ggaaaccact ggatgcgtcg gtgcagcgta atggctccgt acgaaacgag
      841 cgtgtacgcg agtgcgtgct gcccgaacaa cgcgccttgg tgcacatcca tcagcagtcc
      901 gaagacgaag gccgccccca tgccgacacg ccgtggctga tgcacgttcc agaacacgag
      961 tgccagggcc agcacatcag gggcgaaagg cgtacggccc cacggcatca ggttcaccag
     1021 caacgcaacg atcaacgaga accagatgaa gagcggattc gccggtcgca acagatactc
     1081 gcgcggcacg actccacgat cgacgacatc gatcgggtcg gtcggccgca gcgcgaacgt
     1141 gtcgcgaaac cattgctgaa cacgggagat caagctcatc gcttgccggt cccccgtcgc
     1201 gttaccgcct ccggctggac cgggggcgga ggagccggtc gctgcgccgc ctcgaccagc
     1261 agcacgagca gatgggtatg gttttctact cccgcggagg gggtgaggac gatgcgcgca
     1321 aactgatctt tggccgcacg ctcgacgatg ccgaccttgc ccaccggcag ccccgcagga
     1381 tagatcccgt caagccccga ggtgacggca acgtcgccgt tttcgacatc ggcattcgcc
     1441 gccatgaagc gcaggtcgag cgttcccgga tccgcgccgc cgaacgcaat gccgcgcaag
     1501 ccgtttcgca cgatctggat cgggatcgac tgatccttgt cggtaagcag tgtcacttcc
     1561 gcggtgttcg agaagacgcg cgtcacctgg ccgacgacgc cgacatcgtc gatcaccgga
     1621 ttgcctgccc gcacgccgtc gaccgtaccg acgtgcagaa cgatcttgtg cgagaagcgg
     1681 tcgcgcgatt cgtagagcac tgtgcccacg atcgcgccat ggccagtccg ctcgcgggcg
     1741 ccgaggagct tacgcaggcg ctcgttctcg gcaccgacct gctgcgactg aagcagcgat
     1801 tgcgccgact cgacctggcg gcgcttgagc tgctcgtttt cccgctgcag tgtcgtgacg
     1861 gacgtaaagt aatcgcccac cgcatctgcc atgcgtcccg ggaagagcag cacctgctgc
     1921 accgggtaga gaacgacgcc gacgccaacg cggaccgttt ccagcgcctt gatgcgcgaa
     1981 tcgatgacga tcagtgcgat cgccaggaac gagaagaaag cgagccgcgc gcgggcggat
     2041 acaccctgat tgaacagcgg cggcggaccg tattccatta gttgggcgcg cgaaagtgca
     2101 aacctacttt cgcgcgacta gtcagcaaaa gatcgggggc ggagccacac gatcttttga
     2161 tgttcaaagg caggggaaac acgacgagcg acggcgtcgt gtcggggtca gccggtggcg
     2221 aagatggtgc cgagcttgtc catgcgctcg agcgcgatac cgcagccgcg cacgacgcag
     2281 gtgagcggct cctcggccac gaccaccggc aggccggtct cttccatcag cagacgatcg
     2341 agatcgcgca gcagcgcgcc gccgccggtg agcatcatgc cgcggtcggc gatgtcggcg
     2401 ccaagttccg gcggcgtctg ctcgagcgcg atcttcacac tggaaacgat ctggttcaac
     2461 ggatctgtca gtgcctcgag aatctcgttg ctcgaaatcg tgaagctgcg cggtatgcct
     2521 tccgacaggt tgcgcccctt gacctcgatc tccttgacct ccgaacccgg gaacgcggaa
     2581 ccgatgttct tcttgatcga ttcagcggtc ggctcgccga tcagcatgcc gtagttgcgg
     2641 cggatgtagt tgatgatcgc ttcgtcgaac ttgtcgccgc cgacacgcac gctgcctttg
     2701 tagaccatgc cgccgagcga gatcacgcca acctcggtgg taccgccacc gatgtcgacc
     2761 accatcgagc ccgacgcttc ggacaccggc aacccggcgc cgattgcggc ggccatcggc
     2821 tcttcgatca ggaacacctg cgaggcaccg gccgcttccg ccgattcctt gatcgcgcgt
     2881 cgctcgacct gcgtcgagcc gcacggcacg cagatgatga tgcgcgggtt cggcgcgaac
     2941 aggctcgacg ggtggaccat cttgatgaac tgcttgagca tctgctcggt caccgtgaag
     3001 tcggcgatca cgccatcctt catcgggcgg attgcctcga tgttgccggg caccttgccg
     3061 agcatctgct tggcttcctt gccgacggcc tggatcgtgc gcttgccgtt aggcccgccc
     3121 tcctggcgga tcgcaacgac cgaaggttca tcgagcacga tgcccttgcc gcgcacgtag
     3181 atgagcgtgt tcgcggtgcc aaggtcgatc gccaggtcgt tggagaaata gctgcggaga
     3241 aaaccgaaca tgactacctt ttttggctga tctgcgtccc ggagcgcccc gcccgccggc
     3301 gctgcgttct ctcggaagca tgcagagtct gtggagaatg cgcgatgata aactaatcac
     3361 acgacaaacc cgctgcaaac gcttgttttg aaaggcttac gcagcggttg tccaccccgc
     3421 atttccgctg ttaccgactc tcacccctcc tcgatggcac tcgacctcgc gcaaatccgc
     3481 cgcattgccg acctggcgcg catcgacgtc acggacaccg aggcccagcg cgtgctcgat
     3541 cagctgaacg acatcttccg gatgatcgag cgcatgcagg cggtcgacac cgatgccctc
     3601 gagccgatgg cgcacccgct gggcggcgcg cagcggctgc gagaagacga agtgaccgtc
     3661 ggcgacgagc gcacggccaa ccttgcgaac gctcccgagc agcaggacgg cctgttcctg
     3721 gttccgcgcg tcatcgaatg agcgcagcgc cgggccgtcc caagcaagct cgcgccgcag
     3781 tgcgcagcac ggaggctcag ggcgcgagcg cagcgccgga cacccacgac gcaaaggtgc
     3841 tccagtgagc gcacttgatc tcggacaggc cacgctggcc gacctgtcgc gactgctggc
     3901 ggcgcgcgaa gtgtcatcgg tggaacttgc gcgcgcgtac ctcgcgcgca tggaagcgca
     3961 gcggtcactc aatgcgttcc tggatgtgcg ccccgaggtc accctggcgc aggccgccgc
     4021 cgcggacgca cgcatcgccc gcggcgaagc aacgccgctg acgggtctgc cgatcgcgca
     4081 caaggatatt ttcgtcacca aggaattcgc ctcgacggcg tcgagcagga tcctgaaggg
     4141 ctatatgagc ccgttcgacg cgaccgtggt cgacaacctg tcgcgcgccg gcatggtcac
     4201 tctgggcaag ctgaattgcg acgagttcgc aatgggctcg agcaacgaga acagcgccta
     4261 cggcaatgtg ctgaatccgt gggaccacgg cgcagtgccc ggcggctctt ccggcggatc
     4321 ggccgcggcc gtggccgcgc gcattgcgcc ggtggctacc gccaccgata ccggcgggtc
     4381 tatccgcgag cccgcggcct tcagcggcat caccggcacc aagcccacct acggacggcc
     4441 gtcgcgctgg ggcatgatcg cgttcgcgtc gagcctggat acggccggcc tgatgacgct
     4501 gtcggccgag gacgcggcgc tggtgttcaa cgcgatgctc ggcttcgacc cgaaggactc
     4561 gaccagcctg gaccgtccga ccgaggatta cacgcgttcg attggtgctt cggtcaaagg
     4621 catgaggatc ggcgtaccca aggagttttt cgggacggga ctgcagccgg acgtcgaggc
     4681 tgcagtacgt gccgcactcg tcgagtacga aaagctcggc gcccggctcg tgccgatttc
     4741 gctgcccaat gccgaactcg gcatcccggt ctactacgtg atcgcgccgg ccgaagcatc
     4801 gagcaacctt gcgcgcttcg atggcgtgcg ctacgggcat cgggcggcga agtacacgga
     4861 cctcaaggac atgatccgca agtcgcgtgc cgaaggcttc ggcgacgaac cgaagcgccg
     4921 catcctgatc ggcacctatg tgctgtcgca cggctactac gacgcctact tcatcaaggc
     4981 gatgaaggtg cggcgtctga tcgccaatga attccagcag gcctttgccc ggtgcgacgt
     5041 catcgccggt cccgtgacga ccagtgtcgc gtttggcttc ggcgagaaag cggccgatcc
     5101 ggtcgcgatg tacatggccg atctttacac ggttcccggc agcctcgcgg gggtgccgtc
     5161 gatgagcatt ccgtgcgggt tcggcaccgg gccggtcaac agccgccgtc cggtcggact
     5221 gcagctgatc gccaactatt tcgaagaagc caaactgctc ggcgtcgctc atgcctacca
     5281 gagcgcgact gactggcacc gacgcgtgcc tgccggcgcc tgaccgcgac catgtcgcaa
     5341 cgccttctgc tcgtcaacca gcaactcgtc gatgcggcgc gcgatccgct ggttcgcgcg
     5401 cggcacctgc aggatcgtgt cgtgctgctc gcgcgcctcg gtcgggtgga gcaggcgcgc
     5461 gcattgctcg agagcgcatt gcgcgagact cctgccgatg cacccgccgc actgcggctg
     5521 cgatttgact acgccagcgc gatccgcctg tacttcgcca agcggttcga cgatgcgcgc
     5581 acggccatgc tggacgtggc tgagcgcgcg cgacacgcgg atggggacat ggcgctggtg
     5641 ggcgaatgcg agtcggcgct ggcgcttttc atgcagcgcg agggcgacgt gcgctctgcg
     5701 gccagctatg cacgcgcggt gcttgccaat cgcgctgcca cgccggagtc gcgctatcgc
     5761 gcgttcctgg cgctgggctc gctgcatcag gatgcctacg actacgagga ggcgggtcgc
     5821 ctgtatcgcg aggccgaggg tctggtgcgc cagctcgacg acgagatcgc gatggcgagc
     5881 tggctgaaac gctcagcgct cacgcaggcc gcgcatgcgc gccaggctgc ggctctcggc
     5941 gaactcgatg cacgcacgct ggccaacgcc gtcgacgcgc tgaaaaagag cattgcattc
     6001 gcgaagggtc tggcggaagg ccccgaagcc atgctcgacc acctgctgct cgccgaaatg
     6061 cacgtgctgc agcgccgcta caccgacgca ctgaccctgt acgacgccca tcttccgagc
     6121 gccgatggcg aagggttcct gcacgaagtc acggcagcga tggccgacag cgcgcactgc
     6181 tgcatcgagc tcggccgcgt cgatgacggc tacgcccagt tgctgagcgc actgcgccgg
     6241 gtcgacgagt cgaccccgtc ggacatccgc gcgatcgtcc acgccaacat ggccgccgcg
     6301 ctgaagaa
//
LOCUS       DATIRT010000172         5671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1400975, whole genome shotgun sequence.
ACCESSION   DATIRT010000172 DATIRT010000000
VERSION     DATIRT010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5671
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400975"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VLN25_04100"
     CDS             <1..888
                     /locus_tag="VLN25_04100"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HSQ09785.1"
                     /translation="LDKCWDELPVDRILTALEHRWMHAETVCYLLHQIEPGKKRAPGS
                     PQGASAAANGIARRPHPWVAIPAGVAILGQAPDAFGWDNEFPQHGYEVADFEISGHKL
                     TNGGYLRFVVAGGPPPHFWRQRDGTWWLRRMFDETPLPLNAPVYLTHRQATAYAAWAG
                     LRLPTEAEWQRAAYGDDERRTPWGDAEPDAARANLDFVEWDPLPVAAHPLSATPLGVE
                     QMVGNGWEWTATPFAAFEGFSPRPYYSGYSANFFDGEHFVLKGASPRTARRLARPSFR
                     NWYRAEYPYVYATVRLARS"
     gene            complement(953..1915)
                     /gene="egtD"
                     /locus_tag="VLN25_04105"
     CDS             complement(953..1915)
                     /gene="egtD"
                     /locus_tag="VLN25_04105"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HSQ09786.1"
                     /translation="MGFADATARMRFIDLLPAGADPAAELRAGLAAPVPWIAPKFFYD
                     ALGARLFEAICELPEYTLPRDERVIFDRCTDEIASLIGPGSTLIDLGAGNCAKAERLF
                     EALQPVQYVAVDSAGPFLRAQLEALAGRHRSIDVIGVAADFSPGLVLPPVVRRERRGV
                     FYPGSSIGNFAPEEARAFLSCIHDQCAGGHLLIGADLVKNPAELVAAYDDALGVTASF
                     NLNVLNVVNRLAGTDFTPGDWRHVALFNAAHRRIEMHLEARRELRVGWPGGERNFAAG
                     ARIHTENSYKTTSASMSDGLRAAGFGSVRLYTDPAGRFALALAQ"
     gene            complement(1986..2531)
                     /locus_tag="VLN25_04110"
     CDS             complement(1986..2531)
                     /locus_tag="VLN25_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09787.1"
                     /translation="MVAARTPNTIHSSGARNTGTSVKKPRNPRRKAISWNAKIRPELE
                     PEIVSDKRLGDRLLLPTPLLVAEEIAQVPRGATITISQLRRRLAQRFSADRTCPLMTG
                     IFVKIIAGAVAEDLTQGRKPRWPVWRLVNDSGTLSTTWPLDALYRATCLREEGVRLGH
                     KGGAWQVLNPGNVAGQGSVLG"
     gene            complement(2652..3857)
                     /locus_tag="VLN25_04115"
     CDS             complement(2652..3857)
                     /locus_tag="VLN25_04115"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HSQ09788.1"
                     /translation="MVTLESIRAAAARLAGRVEVTPCVHSRTLSQITGAEVYLKFENL
                     QFTASFKERGALNKLSLLTPAQRAKGVIAVSAGNHAQGVAYHAERLGIPAVIVMPRFT
                     PMVKIERTRGFGPEIVLTGDTFDDARLEAAKIATARGLTMVHPYDDADVIAGQGTVAL
                     EMLAQQPELDCLCVAIGGGGLISGVATAARALKPGISVIGVQTDQFPSMYAAFKGVDM
                     PSANATLAEGIAVKQPGELTCEIVKRIVDDVVLVSEGDIEQAIVMLLEIDKTVVEGAG
                     ATGLAAVHRHPALFAGRKVGLILCGGNIDPMVLADIIERGMVRAGRLARIRVHTRDVP
                     GELARAATLIGQAGANIEEVEHQRAFTTLPVQNAELEFVLQTRGPEHIEEVLRTLRNA
                     GLYANTHPY"
     gene            3964..4638
                     /locus_tag="VLN25_04120"
     CDS             3964..4638
                     /locus_tag="VLN25_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSQ09789.1"
                     /translation="MLQIHGLTKRYRERAVLDHLDLTVGGGEYVAIVGESGSGKSTLL
                     NLIAGLDRPDAGSVVIDGVDIAALDDTARTLTRRSKLGFVFQAFHILPHLTVAQNVAL
                     PLVLLGVAPEPREQRAADLLAEVGLAGREASMPRELSGGELQRVAVARSLVHQPALVL
                     ADEPTGNLDPDTASVVLQLLAREIRSRHAAGVLVTHSEVAAATADRTYRLDRGRLVPH
                     LATALP"
     gene            4635..>5671
                     /locus_tag="VLN25_04125"
     CDS             4635..>5671
                     /locus_tag="VLN25_04125"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSQ09790.1"
                     /translation="MITWLLAHISGGYARHHPLRAIVQVIAIAVGVALGYAVNLINAS
                     ALDEFSGAVRHVLGQSDFSVSGGRIGFDEQVYARIAALPEVEMASPMVEVEAPVPSQP
                     RSAAGLATLRVVGIDVFRAALLSPAWVGEPDSKSEDGPFKYLSDGLFLSPAALERFGL
                     KPGDPLHLQVGPRTETFSIAGRLTRARAGEELAVLDIAAAQTRFDQTGRLHRIDVKVA
                     AGVAPAMARERIAALLPAGVTISTPDEAAQRVSNVSRAYRINLNVLALVALFTGAFLV
                     FSLQAQAVVARRTELAFLRISGVTAGELQRLLVIEAIVAGVIGSLFGLALGIALAAVA
                     LAALGGDLGSG"
BASE COUNT          928 a         1897 c         1877 g          969 t
ORIGIN      
        1 cttgataaat gctgggatga gctcccggtc gaccgcatcc tgacggcgct cgagcatcgc
       61 tggatgcacg cagagacggt gtgttatttg ctgcaccaga tcgaacccgg taagaaacgc
      121 gcgccgggct cgccgcaggg cgcaagtgca gcggccaatg gaatagctcg acgcccgcat
      181 ccttgggttg ccattccggc gggcgtcgcc atcctgggcc aggcgccgga cgcctttggc
      241 tgggacaacg agtttccgca gcacgggtac gaggtcgccg acttcgaaat ctctggccat
      301 aagctcacca acggcggcta cctgcgcttt gtcgtcgccg gcgggccgcc accgcacttc
      361 tggcgtcagc gcgacggcac atggtggttg cgacgcatgt tcgacgagac accgctaccg
      421 ctcaatgccc cggtgtacct gacgcatcgg caggcgacgg cgtatgcagc gtgggcggga
      481 ctgcgcctgc cgaccgaggc cgaatggcaa cgtgcggcgt atggcgatga cgagcgacgg
      541 actccgtggg gtgatgccga gcccgacgcc gcgcgcgcaa acctcgactt cgtcgaatgg
      601 gatccgctgc ccgtggccgc gcacccgctc agcgcaacgc cgttgggagt agagcagatg
      661 gtgggcaatg gctgggaatg gactgccacg ccgtttgcgg cgttcgaagg attctcgccg
      721 cgaccctact actccggata ttcggccaac ttcttcgatg gcgagcattt cgtactgaag
      781 ggcgcatcgc cacgcacggc acggcgcctg gcgagaccct cgtttcgcaa ctggtaccgc
      841 gccgagtacc cgtatgtcta cgcgaccgtg cggctggcgc gctcctgacc ggtgcgtgag
      901 acgtgtgaac tactggacca ctgcgttact ggaccactgc gttactcgac cactactggg
      961 ccagtgcgag cgcaaagcgg ccagcgggat cggtgtagag ccgcacactg ccgaatccgg
     1021 ctgcgcgcag tccgtccgac attgaagcag acgtcgtctt gtacgaattc tcggtgtgga
     1081 tgcgcgcgcc agcggcgaag tttcgttcac cgcccggcca gccgaccctt agctcgcgac
     1141 gtgcctccag gtgcatttcg atgcggcggt gggctgcgtt gaacaacgcc acgtggcgcc
     1201 agtctcccgg agtgaagtcg gtccccgcaa ggcggttgac gacattcagc acgttcagat
     1261 tgaacgacgc cgtcaccccg agcgcatcat cgtaggcagc gaccagctcg gcgggattct
     1321 tgaccaggtc ggcaccgatc agcagatggc cgcccgcgca ttgatcatgg atgcatgaca
     1381 ggaacgcccg cgcttcctcc ggcgcgaagt tgccgatcga cgatcccgga tagaagaccc
     1441 cccgcctctc gcgacggacc acgggcggca acaccagtcc gggagaaaaa tccgcggcca
     1501 caccgatgac gtcgatgctc cgatgccgcc cggccagcgc ctccagctgc gcgcgcagga
     1561 acgggccggc actgtcgact gccacgtact gcaccggttg caacgcctcg aacagccgct
     1621 cggctttagc gcagttgccg gcgcccaggt cgatcagcgt gctgcccggc ccgatcagcg
     1681 aagcgatctc gtcggtgcaa cgatcgaaga taacccgctc atcccgcggc aacgtgtact
     1741 cgggcagttc gcagatcgcc tcgaacagcc gcgcgccgag tgcgtcatag aagaatttcg
     1801 gcgcgatcca aggtaccggc gcagcgagac cggcgcgcag ctcggcggcc gggtcggcac
     1861 cggccggcaa cagatcgatg aagcgcattc gcgcagtggc atcggcgaaa cccatgccac
     1921 agtctacggg cgccgctccg tagcggcgcc gtcataatgt gtcgaaccgc gcggtcacaa
     1981 cgtggtcagc caagcacaga gccctggccg gccacgttgc cggggttgag cacctgccag
     2041 gcgccgccct tgtgcccgag gcggacgcct tcctcgcgca gacaagtcgc ccgatacagc
     2101 gcatcgagcg gccaggtcgt gctcaacgtg ccgctgtcgt tgacaagacg ccacaccggc
     2161 caacgcggct tgcgtccctg cgtgaggtct tcggccaccg ctccggcgat gatcttcacg
     2221 aagatgccgg tcatcagcgg gcaggtgcga tcggcgctga atcgttgcgc caggcgcctg
     2281 cgcaactgcg agatcgtgat cgtcgcgccg cggggcacct gcgcgatctc ctcggccacc
     2341 agcaacggcg tcggcagcag caggcgatcg ccaaggcgct tgtcgctgac gatttccggc
     2401 tcgagttcgg gacggatctt cgcgttccag ctgatcgcct tgcgacgtgg attgcgcggt
     2461 ttcttgacag aagtgccggt gttccgagcg ccggaactgt ggatcgtgtt cggggttcgc
     2521 gcagcgacca tgccgcctcc ctcttggtgc cgtcgagaga taacttagcg gcgtctgggc
     2581 ttccgacaat gatctggcgc aagcgagcat gggcttgcaa tcacaaagtt gatcgcgcgt
     2641 tgacgatcga cttaatacgg atgagtgttc gcgtacaggc ccgcgttgcg caatgtgcgc
     2701 agcacttctt caatgtgctc cgggccgcgc gtctgcaaga cgaactcgag ttcggcgttc
     2761 tgcaccggca gcgtcgtgaa cgcgcgctgg tgctcgactt cctcgatgtt ggcgccggcc
     2821 tggccgatca gcgtggccgc acgcgcgagt tcacccggca cgtcgcgcgt gtgcacccgg
     2881 atgcgtgcca gccggcccgc gcgcaccatg ccgcgttcga tgatgtcggc caggaccatc
     2941 gggtcaatgt tgccgccgca caggatcaac ccgaccttgc gtccggcaaa gagcgcggga
     3001 tgccggtgta cggccgcgag cccggtcgcg ccggccccct cgaccacggt cttgtcgatt
     3061 tcgagcagca tcacgatcgc ctgctcgatg tcgccttcgg acaccagtac tacgtcgtcg
     3121 acgatgcgct tgacgatctc gcaggtcagc tcgcccggct gcttgactgc gatgccttcg
     3181 gccaacgtgg cgttggccga aggcatgtcg acacccttga acgccgcata catcgacgga
     3241 aactggtccg tctgcacccc gatgacggag atgcccggct tcagcgcgcg cgcggccgtg
     3301 gccacgcccg agatgagccc accgccaccg atggccacgc acaggcagtc cagttccggc
     3361 tgctgcgcga gcatctcgag cgcgaccgtg ccctgcccgg cgatgacatc ggcgtcgtcg
     3421 tacgggtgaa ccatcgtgag gccgcgagcg gtggcgatct tcgccgcctc gaggcgagca
     3481 tcgtcgaagg tgtcgccggt cagcacgatt tcaggcccga agccgcgcgt gcgttcgatc
     3541 ttgaccatcg gcgtgaagcg cggcatcacg atcacggcgg gtatgcccaa gcgctcggcg
     3601 tgatatgcga cgccctgggc gtggttgccc gcggataccg cgatcacgcc cttcgcgcgt
     3661 tgcgccggcg tcagtagcga caacttgttg agcgcaccac gttccttgaa cgaggcggtg
     3721 aattgcaggt tctcgaactt gagatacacc tcggcgccgg tgatctgcga aagcgtgcgc
     3781 gagtgcacgc acggcgtcac ctcgacacgg ccggcaaggc gcgccgcagc agcgcggatc
     3841 gactccagcg tgacgatgtc gctcaacgcg gtctcggttt cggcgaagga aggggcgaga
     3901 ttgtagcggc cggcctcggc gcgcccgtgc agtcgcccaa gaccggcccg tagaatcgat
     3961 cgcatgctgc agatccacgg cctgaccaag cgctatcgcg agcgcgcggt gctcgaccac
     4021 ctggacctga cggtcggcgg cggcgagtac gtcgcgatcg tcggcgagtc gggttcggga
     4081 aagtcaacgc ttctgaatct gattgccgga ctcgaccgac ccgacgccgg ttctgtcgtc
     4141 atcgacggcg tcgacatcgc ggccctcgac gacacggccc gcacactcac gcgccgctcg
     4201 aagctcggat tcgtgttcca ggcgttccat atcctgccgc acctgaccgt cgcgcagaac
     4261 gtcgcgttac cgctggtgct gctgggcgtc gcgccggagc cgcgcgaaca acgcgccgcc
     4321 gacttgctcg ccgaagtcgg cctcgcagga cgcgaagcga gcatgccgcg tgaactgtcc
     4381 ggcggcgagc ttcagcgcgt cgcggtcgcg cgttccctgg tgcaccagcc tgcgctggtc
     4441 ctggccgacg agccgactgg caatctcgac cccgacacgg cctcggtcgt gctgcagctg
     4501 ctggcgcgcg agattcgcag ccgccacgcg gccggagtgc tggttacgca ttcggaggtg
     4561 gccgcggcca cggccgatcg cacctaccgg ctcgaccgcg gacgattggt gccacatctg
     4621 gcgacggcct tgccgtgatc acctggctgc tcgcccacat ttccggcggc tacgcacgtc
     4681 accatccgct gcgtgcgatc gtgcaggtga tcgcgatcgc ggtcggagtc gcactcggct
     4741 acgcggtgaa tctgatcaat gcctcggcgc tcgacgagtt ctcaggcgct gtccgccatg
     4801 tactcgggca gtcggacttc tcggtcagcg gcggacggat cggcttcgac gaacaggtgt
     4861 atgcacgtat agccgcgctg cccgaagtcg aaatggccag tccgatggtg gaggtcgagg
     4921 cgccggtgcc gtcgcagccg cgctccgctg cgggcctcgc gacgctgcgt gtggtcggca
     4981 tcgatgtctt tcgtgcggcg ctgctgtcgc cggcgtgggt cggcgaacct gactcgaaaa
     5041 gtgaagacgg cccgttcaag tatctctcgg atggactgtt cctctcgcca gccgcactgg
     5101 agcgcttcgg actgaaaccg ggcgacccgc tgcatctgca ggtcggaccg aggacggaaa
     5161 cgttctcgat cgccggacgc ctgacgcgcg ctcgcgccgg cgaggaactg gccgtgctgg
     5221 acatcgccgc ggcgcagacg cgattcgacc agacgggccg gctgcaccgc atcgatgtca
     5281 aggtcgctgc gggcgtggcc ccggcgatgg cgcgcgaacg gatcgcggca ctgctgccgg
     5341 ccggggtgac gatcagcacc ccggacgagg ccgcccagcg cgtatcgaac gtgtcgcgcg
     5401 cgtaccgcat caacctcaat gtgctggcgc tggtggctct cttcaccggc gcattcctcg
     5461 tgttctcgct gcaggcgcaa gcggtggttg ctcggcgcac cgaactggcg ttcctgcgca
     5521 tcagcggcgt gacggcaggc gagttgcagc gtctgctcgt catcgaagca atcgttgcag
     5581 gcgtcatcgg cagcctgttc ggtctcgcgc tcggcatcgc gctcgccgca gttgcgctgg
     5641 cggcacttgg cggcgatctg ggcagcggtt t
//
LOCUS       DATIRT010000173         1987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_515267, whole genome shotgun sequence.
ACCESSION   DATIRT010000173 DATIRT010000000
VERSION     DATIRT010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515267"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(375..1742)
                     /locus_tag="VLN25_04130"
     CDS             complement(375..1742)
                     /locus_tag="VLN25_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011578756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HSQ09791.1"
                     /translation="MTDDTLLPFDVPAVRRKKLTVDFDGGNQSSDGGLLLLRQAERKL
                     GVCGRLADAMPDRRDASRIRHEMFEMVMARVSAIACGHKDAIDLDRLRHDPLMKVAVG
                     RCPESGAPLASQSTISRLENAPSRTEAARLTAALIDQTGTTVKPRAQEILDIDDTFCA
                     AHGGQQLAFWNAHHDERGFASMHIYHVASGTPLVTILRPARTPKGTEVRTVIKHVTKR
                     LRRHWPKTRMIWRGDSHYGRVEAMEWAEDNGADYIFGLAGNAALDALMAETADYLRFH
                     HAVSSKAKLRGFASFMYQAGSWKRPRKVVARLECSLQPVAGETGMRQEVDIRYVVTSL
                     KGSAQHLYEKVYCQRGQMENLIKLHKAQLASDRMSCHSATANQVRLVLHTAAFWLMHG
                     VRSAIPQTNPLAKCEFATIRERLIKIGARVIEHIARIRVQLPTSCPEGALFRTVALGL
                     LPSGP"
BASE COUNT          340 a          623 c          623 g          401 t
ORIGIN      
        1 aaggttgatc gcctcataat ttgatatgct tgatgtgggc aacgaatctt cgtcgagatc
       61 ttgacggcca agcatttccc ctacgaattc cattcccaac gaagcgagtt ggcgctttag
      121 ctgagacagc gccttattta agacaaagcc tgccatcatc tcgaatgact cggattgaaa
      181 tgcagtaacg atttggcgct ggtctcgcat gcttagccgc aatcatgcat caggctgagc
      241 atattgcaca tctgtctggc ggctcgcgcg ctggcaatcc gtcctgctgg atcggtgtga
      301 gacaactcgc tgtgcgacgc gtttggattg atctgcctgt gtcggcggct ggtcggggca
      361 caccgccccc cggcttacgg gccggacggc agcaggccga gcgcgacggt tcggaataat
      421 gccccctccg ggcaactggt cggcagttgg acgcggatgc gcgcgatgtg ctcgatcacg
      481 cgcgcgccga tcttgatcag gcgctctcgg attgtcgcaa actcacactt ggcaagcggg
      541 ttggtctgcg ggatcgcgga gcgcacgcca tgcatcagcc agaacgcggc ggtgtggagg
      601 acgagccgca cctgattggc ggtcgcgctg tgacacgaca tgcgatctga cgccagctgc
      661 gccttgtgca gcttgatcag gttctccatc tgcccgcgtt ggcagtagac cttctcgtaa
      721 aggtgctgcg ccgagccctt cagcgaggtg acgacgtagc ggatgtcgac ttcctggcgc
      781 atgccggtct cgcctgccac cggctgcagc gagcactcga gccgagccac tactttgcgc
      841 ggccgcttcc agctgccggc ctggtacatg aagctcgcaa agccgcgcag cttcgccttg
      901 ctgctcaccg catgatggaa gcgcagataa tcggcggtct ctgccatcag ggcgtcgagc
      961 gcggcattgc cggcgaggcc gaagatgtag tccgcgccgt tgtcttcagc ccattccatg
     1021 gcctcgacgc ggccgtagtg gctgtcgccg cgccagatca tgcgggtctt cggccaatgt
     1081 cttcgcaggc gcttcgtcac gtgcttgatg acggtcctca cctccgtacc cttcggggtg
     1141 cgcgctgggc gcaggatggt caccaacggc gtgccgctcg ccacgtgata gatgtgcatc
     1201 gatgcgaagc cacgctcgtc gtgatgcgcg ttccagaacg caagctgctg gccgccgtgc
     1261 gccgcacaga acgtgtcgtc gatgtcgagg atctcctgcg cgcgcggctt cactgtcgtg
     1321 ccggtctggt cgatgagagc ggcggtgagc cgcgccgcct ccgtcctgct cggcgcattc
     1381 tcaagacgac tgatcgtcga ttgcgaggcc agtggtgcgc cgctctccgg acaacgacca
     1441 accgccacct tcatcagcgg gtcatgccgc agccgatcga ggtcgatggc atccttgtgg
     1501 ccgcaggcaa tcgccgagac ccgcgccatc accatctcga acatctcgtg acggatgcgg
     1561 ctcgcatcgc ggcgatccgg catcgcatcg gcaagccgcc cgcaaacgcc gagcttgcgt
     1621 tccgcttgcc gcaacagcag tagaccgcca tccgatgact ggttgccgcc gtcaaaatcg
     1681 actgtgagtt tcttgcggcg gacggctgga acgtcgaagg gaagaagcgt atcgtcggtc
     1741 atggcggctg tggcgctcgg ctgatgcacg gtgcaatttg tttggacaac gatttgctac
     1801 gtgaatcgcc gcctttgtgc cactgccgcc gcataatttc tcacgcctga tgcataatcc
     1861 gggttagggc ggctgcggag ccgggcgctt gaagagattc ggaattaggc cactcagtta
     1921 gcggctcaca tcggcccgga cgtcagcacg ccgggcgtgc tgcgcgccct cgcccgcgga
     1981 tgcccga
//
LOCUS       DATIRT010000174         2962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1401252, whole genome shotgun sequence.
ACCESSION   DATIRT010000174 DATIRT010000000
VERSION     DATIRT010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401252"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2635
                     /locus_tag="VLN25_04135"
     CDS             <1..2635
                     /locus_tag="VLN25_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HSQ09792.1"
                     /translation="AHCAKGASVREHGMTEHSHRLKYPMKLVDGKYQKISWDQAIDEI
                     TAKMSKIREESGPDAAYFIGSSKHNNEQAYLMRKFVSLWGTNNCDHQARICHSTTVAG
                     VANTWGYGAMTNSYNDMMNSKAIMYIGSNAAEAHPVSMLFSLHAKENGAKMIVVDPRF
                     TRTAAKADHYIRLRSGGDIPYLYGMMYHIFKNGWEDKAYLAARVYGIDKVKEEAMKWT
                     PDKVTEACGVPEDQVLLAAETMAKNRPSTIVWCMGQTQHTIGNAIVRTSCLLQLVLGN
                     VGVSGGGTNIFRGHDNVQGATDVGPNPDSLPGYYGIVPGSWAHWAKVWNVDLDWLKKR
                     FASDAMMTKPGTTVSRWIDAVLEPNDSIGQGPNLRAVFYWGHAPNSQTRGLEMKKAMD
                     ALDLLVVVDPYPSATAAMAAMPAGEGGKLNPNRAVYLLPACTQFETVGSVTASNRSLQ
                     WREKVIEPLFESRTDHAIMLRFAQKWGFGDQLLGKRDGTQNIRTIPGAGGWEEPVIED
                     ILANEINRGTWTIGYTGQSPERLKLHMKHMGTFDVKTLRAKGGPCDGDYFGLPWPCYG
                     TPQMKHPGSSNLYDTSRTVMDGGGNFRANFGVERDGVNLLAEAGSHSKGAEITTGYPE
                     FDHVFLKKLGWWNELTEDEKKAAEGKNWKTDLSGGIIRVVMTKHQCHPFGNAKARAVV
                     WNFPDPVPLHREPLFSNRPDLVVKYPTHADRKDFWRVPTLYKTVQDKNIADKVYEKFP
                     LVMTSGRLTEYEGGGDETRSNPWLAELQQNAFVEINPKAANDRGIRNGEWVWVNTPTG
                     AKLKVMALVTERVGPDTVFVPFHFAGWWQGKDLIDSYPAGAAPIVRGEAINTSTTYGY
                     DSVTNMQEGTKTTLCQVAKLA"
     gene            2668..>2962
                     /locus_tag="VLN25_04140"
     CDS             2668..>2962
                     /locus_tag="VLN25_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HSQ09793.1"
                     /translation="MARMKFICDANRCIECNGCVTACKNANDVPWGVNRRHVVTLNDG
                     VPGERSVSVACMHCSDAPCMAVCPVDCFVRSEDGVVLHDKDQCIGCGYCLYVCP"
BASE COUNT          628 a          908 c          935 g          491 t
ORIGIN      
        1 tgcgcactgc gccaagggcg catcggtgcg tgagcacggc atgaccgaac actcgcaccg
       61 tctgaagtac ccgatgaagc tggtcgacgg caagtaccag aagatcagct gggatcaggc
      121 gatcgacgag atcactgcga aaatgtccaa gattcgcgag gagtccggtc ccgacgcggc
      181 atacttcatc ggctcgtcca agcacaacaa cgaacaggcg tacctgatgc gcaagttcgt
      241 ttcgctctgg ggcacgaaca actgtgacca ccaggcgcgc atctgccact ccacgacggt
      301 cgccggtgtg gcgaacacgt ggggttacgg cgcgatgacg aactcctaca acgacatgat
      361 gaactcgaag gccatcatgt acatcggatc gaacgccgcc gaggcgcacc cggtgtcgat
      421 gctgttctcc cttcatgcga aggagaacgg ggccaagatg atcgtcgtcg acccgcgctt
      481 cacgcgcacg gcggcgaagg ccgatcacta catccgcctg cgctcgggcg gagatatccc
      541 ctacctctac gggatgatgt atcacatctt caagaacggc tgggaggaca aggcttacct
      601 tgccgcgcgc gtgtatggca tcgacaaggt caaagaggag gccatgaagt ggactcctga
      661 caaggtgacc gaagcctgcg gagtaccgga agatcaggtg ctgctggccg ccgagaccat
      721 ggccaagaac cggcccagca cgatcgtctg gtgcatgggg cagacccagc acacgatcgg
      781 caacgccatc gtgcgcacat cgtgcctgct gcagctcgtc ctcggaaacg tcggtgtctc
      841 tggtggaggc accaacatct tccgcggcca cgacaatgtt cagggcgcga ccgacgtcgg
      901 cccgaacccc gactccctgc ccggctacta cggcatcgtg ccgggatcct gggcgcactg
      961 ggccaaggtc tggaacgtcg atctggactg gctgaagaag cgcttcgcgt ccgatgcgat
     1021 gatgaccaag cccggcacca cggtcagccg ctggatcgac gcggtgctgg agcccaacga
     1081 ctcgatcggc caggggccga acctccgggc cgtgttctat tggggccacg cacccaactc
     1141 gcagacgcgc ggactggaaa tgaaaaaggc gatggacgcg ctcgacctgc tcgtcgtggt
     1201 cgacccgtat ccgtcggcaa ccgccgccat ggcggcgatg ccggccggtg aaggcggcaa
     1261 gctcaatccg aaccgggctg tctacttgct gccggcctgc acgcagttcg agacggtggg
     1321 ctccgtcacc gcgtccaatc gctcactgca gtggcgcgag aaggtcatcg agccgttgtt
     1381 tgaatcgcgg acggaccacg cgatcatgtt gcgcttcgcg cagaagtggg gcttcggcga
     1441 ccagctgctg ggcaaacggg atggcactca gaacatccgc accattccgg gggcgggcgg
     1501 ctgggaagaa ccggtgatcg aggacatcct cgccaacgag atcaatcgcg gcacctggac
     1561 catcggttac accggacaga gcccggagcg gctgaagttg cacatgaagc acatgggcac
     1621 cttcgacgtg aagacgcttc gtgccaaggg cggaccgtgc gacggcgact acttcgggct
     1681 gccgtggccg tgctatggca cgccgcagat gaagcacccc ggctcgtcca acctgtacga
     1741 cacctcgagg acggtgatgg acggcggcgg caacttccgc gccaactttg gcgtagagcg
     1801 cgacggcgtc aacctgctgg ccgaagcggg ctcgcattcg aagggcgccg agatcacgac
     1861 gggctatccc gagttcgatc acgtgttcct gaagaagctc gggtggtgga acgaactgac
     1921 cgaggacgag aagaaggcgg ccgagggcaa aaattggaag accgatcttt cgggcgggat
     1981 catccgcgtg gtgatgacca agcaccagtg ccatccgttc ggaaatgcca aggcgcgtgc
     2041 cgtggtctgg aacttcccgg atcccgtccc gctgcatcgc gaaccgctgt tcagcaatcg
     2101 tcccgacctg gtcgtcaagt acccgacaca cgccgatcgc aaggatttct ggcgcgtgcc
     2161 gacgctctac aagaccgtgc aggacaagaa cattgctgac aaggtctacg agaagttccc
     2221 gctcgtgatg acctcgggcc gcctgacgga gtacgaaggg ggaggcgacg agacgcgttc
     2281 gaacccctgg ctcgccgaat tgcaacaaaa cgccttcgtc gagatcaatc caaaagcggc
     2341 gaacgaccgc ggtatccgca atggcgaatg ggtctgggtg aacacaccga ccggtgcgaa
     2401 gctcaaggtc atggcgctcg tgaccgaacg cgttgggccg gacaccgtgt tcgtgccgtt
     2461 ccactttgct ggttggtggc aaggcaagga tctgattgac agctatccgg ccggagcagc
     2521 gccgatcgtc cgaggcgaag cgatcaacac cagtacgacc tacggctacg actcggtgac
     2581 gaacatgcag gaaggcacga agacgaccct ttgtcaggtt gccaagctgg cctgagaggc
     2641 ctcgagaacg aaacaaggag actcacgatg gcccgcatga agttcatctg tgatgccaac
     2701 cgctgcatcg aatgcaacgg ctgtgtcacg gcctgcaaga acgcaaacga cgtcccctgg
     2761 ggtgttaatc gccggcatgt cgtcactctc aatgacggtg tacctggcga acgctcggtc
     2821 tcggtcgcct gcatgcactg ctccgatgct ccgtgcatgg cggtatgccc ggtcgactgc
     2881 ttcgtccgca gcgaagacgg cgtcgtgctg cacgacaagg accagtgcat cggctgcgga
     2941 tactgcctgt atgtctgccc ct
//
LOCUS       DATIRT010000175         6230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_615026, whole genome shotgun sequence.
ACCESSION   DATIRT010000175 DATIRT010000000
VERSION     DATIRT010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615026"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VLN25_04145"
     CDS             complement(<1..247)
                     /locus_tag="VLN25_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HSQ09794.1"
                     /translation="MLTRDRWTLMERFFETVVAKSREIFSAAERDTEARVKSLLPPIE
                     LQVREQRAQLKKRAESISKIRDAQGSLDERISELELAL"
     gene            complement(305..1846)
                     /locus_tag="VLN25_04150"
     CDS             complement(305..1846)
                     /locus_tag="VLN25_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HSQ09795.1"
                     /translation="MAQLASSFEEYGQWRDALAAAIVQLRQWLVDAQMLDPDAARRVD
                     VALDRLAEDKLVIAFVAEFSRGKSELINAIFFADYGKRILPSSAGRTTMCPTELLYDE
                     SMPPSIRALPIETRSRLGSTSDFKRLSEEWQVFPLDTSSADGMLNAFKIVAETVRVPV
                     DEARSYALFDADDPDQAIALDKNGMVEISKWRHAVINFPHPLLKHGLVILDTPGLNAI
                     GTEPELTLSLVPNAHAVLFVLAADTGVTKSDLDVWRHVVGDAGHRNHIAVLNKIDGLW
                     DVLKTPGEIELEVRHQCAAVAATLDLAPMRVFPVSAQKGLVAKVQNDPTLLEASRLPL
                     LEEALIDLLVPTKQQIVRDQTVAQVERVASEARQTLNARERGVIEQLYELRGLQGKNQ
                     SSIERMTRRALAEQREFEEVVRKIVAARVVLQKLSDKLFVPLRVSTLRAQVLATRNKM
                     KKSQLSPSISGAVRDYFGSLRELLRDANAKALEIEQMVLGVQRRFAEELGWSLSPPMT
                     FSLDT"
     gene            complement(1985..2815)
                     /gene="mutM"
                     /locus_tag="VLN25_04155"
     CDS             complement(1985..2815)
                     /gene="mutM"
                     /locus_tag="VLN25_04155"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HSQ09796.1"
                     /translation="MPELPEVEVTRRALAPYVEGQTIESVDVRDTRLRWPIPHDLATR
                     LSGQRVVCLERRGKYLLWEFAQGTLISHLGMSGAWRVWPRDAAPAARRHDHVDIVVPR
                     YVVRLTDPRRFGALLWHSAAAGPVKEHPLLADLGMEPFDPRFDGAVLYMGTRRRTAAI
                     KQVLLAGKIVVGVGNIYASEALFRAGINPKLAAGRIGPERCDRLAAAVREVLTEAIGV
                     GGSTLRDFVSAAGDEGYFMLDAKVYERAGQPCRVCATPIRRRVQGQRSTYYCPHCQRR
                     "
     gene            2911..4722
                     /locus_tag="VLN25_04160"
     CDS             2911..4722
                     /locus_tag="VLN25_04160"
                     /inference="COORDINATES: protein motif:HMM:NF016611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSQ09797.1"
                     /translation="MLFSFARRAATGSRLFPFLLATAILGCAARPDAAPPMPVPVPVA
                     PVATGPVAPSPSAARPGAIDGIPEVPITGALMYQLMAAELAAQQGELGAAYSIYLKLA
                     RETRDPRLARRAAELALQGRALPQSVEAAELWRQLAPDSREAGQALALLYASTSRFDE
                     AYTLLAAEVKAAASPAAELSRIQRQLARAQDRQGTFKLLERLAQPYLNSAEVRLVLAS
                     GANAAGMTARASEEAKAAVELQPDSERAVLAAAQFLQSTDRAAALELLQRQVARPGAS
                     AEVRLAYARLLVADRQYGPARAQFELLQQADPKNPDLVYSLALLSLQGNLRAEARTYL
                     LRYLDLLQSQPSEERDPDQAFLYLAQLAEDDKQYDDALRWLRKIEGGDEFIPARVREA
                     LVLARMQRADEARKVLRGVQANSPAERLQLVLAEAQLLRETRRYDEAFKLLAQALEKT
                     PDNIALLYDSAMTAEKLDRIEAMERQLRRVIELKPDYAHAYNALGYTFADRNVRLPEA
                     LQLIEKAHQLAPEDPFILDSLGWVYFRMGDLPQARKYLELAYDAKPDTEVTIHLAEVL
                     WSSGDQARARTLLREARAKEPGNELLKSTLARLRIGL"
     gene            4722..5258
                     /locus_tag="VLN25_04165"
     CDS             4722..5258
                     /locus_tag="VLN25_04165"
                     /inference="COORDINATES: protein motif:HMM:NF015512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HSQ09798.1"
                     /translation="MAASRALIALLALALGGCATAPTAPPERSYAGRFSAIADNGAQR
                     DSVSGRFTIEVRGDRKTIDLATPVGTTIARIEIEPGRASATGPQLQTVTGPDADELIE
                     RLLGWRLPVAGLVDWINGRHVPERPARTRRDGDRISEIEQDGWTIRIDEYSPTTGDHH
                     DSSWTARRSDPTRPSPSA"
     gene            5293..6183
                     /gene="ispE"
                     /locus_tag="VLN25_04170"
     CDS             5293..6183
                     /gene="ispE"
                     /locus_tag="VLN25_04170"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016475221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HSQ09799.1"
                     /translation="MDRALALPAPAKINLFLHVLGRRTDGYHDLQSVLCLIDLADWID
                     IARRDDGQLRRSGDVVGAPDDDLALRAARLLQQHTGTRLGADIAITKRIPAGAGLGGG
                     SSDAATTLIALNRLWDTGLDREHMAELGLALGADVPFFVHGTNAFAEGRGERLSPLTL
                     APAHYSVIWPQVHVSTKEIFEDPGLTRNTKATKMSDFSAVVGAVVPASAGECTNGVST
                     ALFGTNDLEPVTCRRYPAIDQVLAHLSGFGTARMTGSGSAAFAVTPSAEAAQAAIAHL
                     PPGWQGWAVCGLQEHPLRAW"
BASE COUNT         1087 a         2065 c         2005 g         1073 t
ORIGIN      
        1 cgagcgcgag ctcgagttcg ctgatgcgtt catcgagcga gccctgcgca tcgcggatct
       61 tggagattga ttcggcacgc ttcttcagtt gcgcccgttg ctcgcggacc tgcagctcga
      121 tcggaggcaa cagcgacttg acccgggcct cggtatcccg ttcggccgcg gagaagatct
      181 cgcgcgactt cgccacgacg gtctcgaaga agcgctccat caatgtccag cgatcgcgcg
      241 tgagcacggc aaacgcaccg aactgcgaac ggtaggccgc ctcagtgcgc tctaggtcgg
      301 cgatttaggt atcgagcgag aacgtcatcg gcggactcag gctccagccc agttcctcgg
      361 cgaagcggcg ctgcacgccc agcaccatct gctcgatctc gagcgccttt gcattggcat
      421 cacgcagcaa ttcgcgcagg ctgccgaaat agtcgcgcac ggcaccggag atggacggcg
      481 acagttgaga cttcttcatc ttgttgcgtg tcgcgaggac ctgtgcacgc agcgtgctga
      541 cgcgtagcgg cacgaacagc ttgtccgaca gtttctgcag cacgacgcgt gccgcgacga
      601 tcttgcgcac cacttcctcg aactcgcgct gctcggccag cgcccgccgc gtcatgcgct
      661 cgatggagct ctggtttttg ccctgcaggc cgcgcaattc atagagttgc tcgatcacgc
      721 cacgctcgcg cgcgttcagg gtctggcgcg cttcggaggc gacacgctcg acctgcgcca
      781 ccgtctggtc gcgcacgatc tgctgtttgg tcggcaccag cagatcgatc agcgcctctt
      841 ccaacagcgg cagccggctg gcttcgagca acgtcggatc gttctggacc ttggcgacca
      901 ggcccttctg cgcgctgacg ggaaacacgc gcatcggcgc gaggtcgagg gtggccgcga
      961 ccgcggcaca ctggtgccgc acttccagtt cgatctcgcc cggcgtcttc agcacgtccc
     1021 acagtccgtc gatcttgttg aggaccgcga tatggttccg gtggcccgcg tcgccgacga
     1081 catgcctcca gacatcgagg tcgctcttgg tcactccggt gtcggctgcg aggacaaaca
     1141 gcacggcatg cgcattcggc actaatgaca gcgtcagttc cggctcggtg ccgatcgcat
     1201 tcaagcccgg cgtgtcgagg atcaccagtc cgtgcttgag cagcggatgc gggaagttga
     1261 tgacggcgtg gcgccacttc gagatctcga ccatgccatt tttatcgagc gcgatggcct
     1321 ggtcgggatc gtcggcatcg aacagcgcgt aactacgcgc ctcgtcgacc ggaacccgca
     1381 cggtttcggc gacgatcttg aaggcgttga gcatgccgtc ggcagaactg gtgtcgagcg
     1441 ggaacacctg ccactcttcg gacaggcgct tgaagtccga ggtcgagccg agacggctgc
     1501 gcgtttcgat cgggagtgcg cggatggacg gcggcatcga ctcgtcgtac aagagctccg
     1561 tcgggcacat cgtcgtgcgc ccggccgacg acggcaggat ccgcttaccg tagtctgcga
     1621 agaaaatggc gttgatcagt tccgacttgc cgcgcgagaa ctcggcgacg aaagcaatga
     1681 cgagcttgtc ctcggcgagt cgatcgagcg cgacgtcgac gcggcgtgcc gcgtccggat
     1741 cgagcatctg tgcgtcgacc agccactgtc gcaactggac gatggccgct gccagcgcgt
     1801 cacgccactg gccgtattcc tcgaagctgg aagcaagctg ggccatgagc cacgcgtcga
     1861 agcagggtcg agccaatcta tcacagggtc tgcggcacgc gatccgtgcc catgacatga
     1921 aaacgtacca acatccagtt caaatcgagg cacgccccaa ccctcgcgca gcgcgagccc
     1981 cagttcaacg cctctggcag tgcggacagt agtaggtcga ccgttgcccc tggacccgtc
     2041 gtcgtatcgg cgtcgcgcag acgcggcaag gctgccccgc gcgctcgtaa accttggcat
     2101 cgagcatgaa gtagccctcg tcgccggccg ccgaaacaaa gtcgcgcaag gtgctgccgc
     2161 ccacgccgat cgcctcggtc aagacttcgc gcacggcggc cgcaagccgg tcgcagcgtt
     2221 ccgggccgat ccggcccgcc gccagtttgg gattgatgcc ggcacgaaac aacgcttctg
     2281 acgcatagat gtttccgacg ccgacaacga tcttcccggc cagcaagacc tgcttgatcg
     2341 cggccgtccg tcggcgcgtg cccatgtaaa gcaccgctcc gtcgaagcgc ggatcgaacg
     2401 gctccatgcc gaggtcggcc agcaacggat gttccttcac gggtccggcc gcggcgctat
     2461 gccagagcag cgctccgaag cgacgcgggt cggtcagtcg aacaacatag cgtggcacca
     2521 cgatgtcgac gtgatcgtgc cggcgcgcag cgggtgccgc gtcacgcggc cacacgcgcc
     2581 atgcacccga catgccgagg tgggagatca gcgtgccctg cgcgaactcc cagaggagat
     2641 acttgcctcg gcgttccaga cacacgacgc gctgcccgct gagacgggtc gcaagatcgt
     2701 gcgggatcgg ccagcgcaga cgggtatcgc gcacatccac ggattcgatc gtctggcctt
     2761 cgacatacgg agccagcgcc cgtcgcgtga cttcgacttc aggcagttcg ggcatcgcta
     2821 ttccaccgaa cgggcagcgg tcgttctgac ggtcgttctg ataatgtggg gttcattccg
     2881 cttgccgtgc gctccaacgc acgcgattcc atgctgtttt ccttcgcacg tcgcgctgcc
     2941 accgggtcgc gtctttttcc gttcctgctc gcgacggcga ttctagggtg cgccgcgcgg
     3001 cccgatgccg cgccgcccat gcccgtgccc gtgccggttg caccggtagc caccgggcct
     3061 gttgcgccga gtccgtccgc agcgcgccca ggtgcgatag atggcattcc cgaggtgccg
     3121 atcaccggtg cgcttatgta ccagttgatg gcggcggaac tggcggcgca gcagggcgaa
     3181 ctcggcgcgg cctactcgat ctatctgaaa ctggcgcgcg agacgcgcga cccccgactc
     3241 gcgcggcgcg ccgcggaact cgcgctgcag gggcgcgcgc tgccgcagag cgttgaagcg
     3301 gcggaactgt ggcggcagct tgcacccgat tcccgggaag ccgggcaggc actggccctg
     3361 ctttatgcaa gcacgagtcg tttcgatgag gcctacacgc tgctcgcggc cgaggtcaag
     3421 gcggccgcgt cgcccgcggc ggagctgtcg cgcatccagc ggcagctggc gcgcgcgcag
     3481 gaccgacagg gcacatttaa gctgctcgaa cgcctggcgc agccttacct gaacagtgcc
     3541 gaagttcgcc ttgtactggc atcgggtgcg aacgcggccg gcatgacggc acgggcgtct
     3601 gaagaggcca aagcggccgt ggagctgcag cccgattccg agcgagccgt gctggccgct
     3661 gcacagttcc tgcagagcac cgatcgtgcc gcggcgctcg aactgctcca acgccaggtc
     3721 gcgcggcccg gcgcttccgc tgaagttcgc ctggcctatg cacgtctgct ggtcgcggat
     3781 cgccagtacg gacccgcacg cgcccagttt gaactgctgc agcaagccga tcccaagaac
     3841 ccggaccttg tgtactcgct cgcactgctt tccctgcagg gcaatctgcg agccgaagcc
     3901 cgcacctacc tgctgcgtta cctggacctg ctgcagagcc agcccagtga agagcgcgat
     3961 ccggaccagg cattcctgta cctcgcgcaa ctggcggaag acgacaagca gtacgacgac
     4021 gcgctgcgct ggctgcgcaa gatcgagggc ggcgacgaat tcattccagc gcgcgtacgc
     4081 gaggcgctcg tccttgccag gatgcagcgc gcagatgaag cacgcaaagt gctgcgcggc
     4141 gtgcaggcga attcacccgc tgaacgattg cagctggtgc tggccgaagc ccagttgctg
     4201 cgcgagacgc ggcgctacga cgaggcgttc aagctgctgg cccaggcact cgagaagacg
     4261 ccggacaaca tcgcgctgct ctacgacagt gcgatgactg ccgaaaagct cgatcgcatc
     4321 gaggcgatgg agcggcagct gcggcgggtg atcgagctca agccggatta cgcacacgcg
     4381 tacaacgcgt tgggttacac ctttgccgat cgcaacgtgc ggttgcccga agcgttgcag
     4441 ttgatcgaaa aggcgcacca gctcgccccc gaggacccct ttatcctcga ttcgcttggc
     4501 tgggtctact tccgcatggg cgacctgccg caggcgagga agtacctcga gcttgcctac
     4561 gacgcgaagc cggacaccga ggtcacgatc catcttgcag aggtgctctg gtcaagcgga
     4621 gaccaggcac gcgcccggac gctgttgcgt gaggcccgcg cgaaggagcc cggcaacgag
     4681 ttgctgaagt cgacgcttgc ccgcctgcgc atcgggctgt gatggcagcg tcgcgcgcat
     4741 tgatcgcgtt gcttgcgctc gcccttggtg gctgtgccac ggctccgacc gcgccgcccg
     4801 aacgctccta cgccggccgc ttttccgcaa tcgccgacaa tggcgcgcag cgtgacagcg
     4861 tcagcggacg cttcacgatc gaagtgcgag gagaccgcaa gacaatcgac ctcgccacgc
     4921 cggtgggcac cacgatcgcc cgcatcgaaa tcgagcccgg ccgtgccagc gcgaccggac
     4981 cccagcttca gaccgtgacg ggtccggatg ccgatgaact catcgagcgt ttgctcggct
     5041 ggcgcctgcc ggtggccgga ctggtcgact ggatcaatgg ccgacacgta ccggaacgtc
     5101 ctgccaggac tcgacgcgac ggcgaccgca tcagcgagat cgagcaggac ggctggacga
     5161 tccgcattga cgagtattcg ccgacgacgg gcgaccatca cgactcgtca tggaccgccc
     5221 gccgttcgga ccctacccgg ccgtctccgt ccgcgtgatc gtcgacgagc cgacctcctg
     5281 accctggcac gcatggatcg cgcactcgca ttgcccgctc cggcaaagat caatctcttc
     5341 ctgcacgtgc ttggccgtcg cactgacggc tatcacgacc tgcaatcggt tctctgcctg
     5401 atcgatctcg cggactggat cgatatcgcg cggcgcgacg atgggcagtt gcgacggtcc
     5461 ggcgacgtgg tcggcgctcc cgacgacgac ctcgcgctgc gtgcggcccg cctgctgcaa
     5521 cagcacaccg gaacgcgtct tggcgccgac attgccatca cgaaacgtat tccggcgggt
     5581 gccgggctcg gcggcggctc gtccgatgcc gcgacaacgc ttatcgccct gaatcgcctg
     5641 tgggacaccg ggctcgaccg ggagcatatg gccgagctcg ggctggcgct gggcgccgat
     5701 gttccgttct tcgtgcacgg gacgaatgca tttgccgaag ggcgcggcga acgcctgtcg
     5761 ccgctcacgc tcgcgcccgc gcactattcg gtgatctggc ctcaagtgca tgtttcgaca
     5821 aaggaaatct ttgaagaccc cggtttgaca cgaaatacca aagccacgaa aatgtcggac
     5881 ttttctgctg tggtcggtgc cgtcgtgccc gcgagcgcag gcgagtgcac caacggcgtt
     5941 tccacagcac ttttcggcac caatgatcta gagccagtga cgtgccgtcg gtatcccgca
     6001 atcgatcaag tgcttgcgca tctttccggt ttcgggacgg cgcgcatgac cgggtcgggg
     6061 tcggcagcgt ttgccgtcac cccgagtgca gaggcggcgc aggcagcgat cgcccatctg
     6121 ccgccgggtt ggcagggctg ggcggtttgc ggattgcagg agcatccgct gcgcgcgtgg
     6181 tagagagcaa aagtgcggtt cgattcaccg aaccgggcag aacggaagtg
//
LOCUS       DATIRT010000176         7350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1598017, whole genome shotgun sequence.
ACCESSION   DATIRT010000176 DATIRT010000000
VERSION     DATIRT010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7350
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598017"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VLN25_04175"
     CDS             complement(<1..629)
                     /locus_tag="VLN25_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010814997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HSQ09800.1"
                     /translation="MIAQELEVSLHMAFVEARQARHEFITVEHLLLALLDNPSAAEVL
                     RACACNIEDLRKNLQNFIADNTPILPPNSEADTQPTLGFQRVIQRAIMHVQSTSNGKK
                     EVTGANVLVAIFGEKDSHAVYYLHQQGVTRLDVVNFISHGITKSGQGEEKAKPAAEQE
                     QEGEGKDQPSPLEQYTQNLNALAKEGKIDPLIGREPEVERVIQVLCRRR"
     gene            complement(626..934)
                     /gene="clpS"
                     /locus_tag="VLN25_04180"
     CDS             complement(626..934)
                     /gene="clpS"
                     /locus_tag="VLN25_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HSQ09801.1"
                     /translation="MSERIDEGTVVEKQTSRVKPPPMYQVVLLNDDYTPMEFVVGVLQ
                     KFFGKGREQATQIMLKVHHEGRGVCGVYPRDIAATKVEQVCSYSRQHQHPLQCVMEEA
                     "
     gene            1356..1562
                     /gene="cspD"
                     /locus_tag="VLN25_04185"
     CDS             1356..1562
                     /gene="cspD"
                     /locus_tag="VLN25_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008156 - negative regulation of DNA
                     replication [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein CspD"
                     /protein_id="HSQ09802.1"
                     /translation="MATGTVKWFNDAKGYGFITPDDGGEDLFAHFSAIQMNGFKTLKE
                     GQKVQFEVVQGPKGKQASNITAPA"
     gene            complement(2022..3269)
                     /gene="icd"
                     /locus_tag="VLN25_04190"
     CDS             complement(2022..3269)
                     /gene="icd"
                     /locus_tag="VLN25_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HSQ09803.1"
                     /translation="MYAHIKVPTTGQKITVNKDYSLSVPDQPIIPYIEGDGTGVDITP
                     VMIKVVDAAVAKAYGGKKKIVWMEIYAGEKSTKVYGPDVWLPEETLTVLRDYVVSIKG
                     PLTTPVGGGIRSINVALRQELDLYVCLRPIQYFKGVPSPVKEPEKTHMVIFRENSEDI
                     YAGIEYEAESDKAKKLIKFLQDEMGVKKIRFPATSGIGIKPISREGTERLVRKALQYC
                     IDNKRKSLTFVHKGNIMKFTEGAFAKWGYELAQREFKAELLDGGPWMKIKAPHGDIVL
                     KDVIADAFLQQILLRPAEYDVIATMNLNGDYISDALAAQVGGIGIAPGANLSDSVAMF
                     EATHGTAPKYAGKDYVNPGSEILSAEMMLRHMGWTEAADLVIKSMERSILSKKVTYDF
                     ARLMEGATQVSCSGFGQVMIEHM"
     gene            3517..3846
                     /locus_tag="VLN25_04195"
     CDS             3517..3846
                     /locus_tag="VLN25_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09804.1"
                     /translation="MKKVIAALISALFAGAVLAQAAAPAAPAKAATPATPASPATPAA
                     PSAAPATPATPAAAPAAKADAKATTDKKAKSTKKTTTSKKTMTDKKATTDAKPKADAA
                     ATDKPMK"
     gene            3968..4432
                     /locus_tag="VLN25_04200"
     CDS             3968..4432
                     /locus_tag="VLN25_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HSQ09805.1"
                     /translation="MSDPSTLLRRFYTLLLSLALAGSAYAQSGPQPTLPTVRLSAGMH
                     VITAELATTPQSRTIGMMFREKAAPNHGMLFVFEYKAQQCFWMRNTPLPLSIAFIDED
                     GTILQISDMAPKSEALHCSQRPVRFALEMEQGWFARKGAGVGAKIGGLPGTR"
     gene            complement(4489..5070)
                     /gene="sodB"
                     /locus_tag="VLN25_04205"
     CDS             complement(4489..5070)
                     /gene="sodB"
                     /locus_tag="VLN25_04205"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HSQ09806.1"
                     /translation="MEHTLPALPYATDALAPHMSKETFEYHYGKHHQAYVTNLNNLIK
                     GTEFESLSLEDIVRKASGGVFNNAAQVWNHSFFWNCMKPGGGGEPSGALAQAIAAKWG
                     SYGAFKEAFAKSAVGNFGSGWTWLVKRADGSVDIVNTSNAATPLTTADKALLTVDVWE
                     HAYYIDYRNARPKFVEAFLASLVNWDFAARNFG"
     gene            complement(5138..6514)
                     /gene="xseA"
                     /locus_tag="VLN25_04210"
     CDS             complement(5138..6514)
                     /gene="xseA"
                     /locus_tag="VLN25_04210"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012329091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HSQ09807.1"
                     /translation="MALDSELADSGLARSPLTVSALNRAVAGLLERSFPLVRVQGEVG
                     NLTRAASGHWYFTLKDDIAQVRCVMFRGRNSLLDWAPREGDEVEATSIVSLYEARGEF
                     QLNVEFMRRAGLGRLYEEFLRLKQRLAAEGLFDLARKRTLPRLPMCVGVVTSLQAAAL
                     RDVITALGRRAPYVGVIVYPVPVQGAGAGAEIAATLARVSGRRDVDVVLLVRGGGSIE
                     DLWAFNEEAVARAIRACVLPVVVGVGHESDVTIADFAADVRAPTPTAAAELVATSADA
                     LQELLANQMRHLARQLQTRLQAASQRIDFAQRSLATPRAPLAAYDARIALLRSRAAGA
                     IVQATARAAARLQAQHARWLQVRPSRDQEGLLGQRNRLVALGRALLNDNAMRLGPLMA
                     RLATLDPTAVLRRGYAIALDGSGRAVTRAQDLAVGARLRLVLAEGAAGVRVESLQGDG
                     PDGVAGGH"
     gene            6820..>7350
                     /locus_tag="VLN25_04215"
     CDS             6820..>7350
                     /locus_tag="VLN25_04215"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HSQ09808.1"
                     /translation="MLAIIIELGWPVWPLILASVLALALIIERTFALRRIRILPSGLF
                     EDVVGLVKKQTVTPEILVKLEHNSPLGRVLSAGLRNSRVLREGAMQQIEAAGAVVANR
                     LTKNLTLLGSIGSIAPLLGLFGTVVGMIQIFASQQAGSSPQELARGISVALYCTAFGI
                     IVAVPAVLAHRYFRRLV"
BASE COUNT         1324 a         2400 c         2263 g         1363 t
ORIGIN      
        1 ttgcgtcggc ggcacaggac ctggatcacg cgctcgacct cgggctcgcg cccgatcagc
       61 ggatcgatct tgccttcctt ggccagcgca ttgagattct gtgtgtactg ctcgagcggc
      121 gacggctggt ccttgccctc gccctcctgc tcctgctcgg cggcgggctt ggccttttct
      181 tcgccctgcc ccgacttggt gatgccgtgc gagatgaagt tgacgacatc aagtcgcgtg
      241 acgccctgct ggtgcaggta atacacagcg tgcgagtcct tctcgccaaa gatcgcgacc
      301 agcacgttgg cacccgtgac ttccttcttg ccattgctcg tgctctgcac gtgcatgatg
      361 gcgcgctgga tcacgcgctg gaagccaaga gtgggctgcg tgtcggcctc ggagttcgga
      421 ggcaggatcg gcgtgttgtc ggcgatgaag ttctgcaggt tcttgcgcag gtcctcgatg
      481 ttgcatgcgc aggcacgcag cacctccgct gccgacggat tgtcgagcag cgcgagcagc
      541 aggtgctcga cggtgatgaa ttcgtgccgg gcctggcgcg cctccacaaa ggccatgtgc
      601 aggctgactt cgagttcctg ggcgatcatg cttcctccat cacgcactgc agcggatgct
      661 gatgctggcg tgaataactg cagacttgct cgaccttggt cgcggcgata tcgcgcgggt
      721 agacgccaca gacgccgcgt ccttcgtgat gcaccttcag catgatctgg gttgcctgtt
      781 ctctcccttt gccgaagaac ttctgcagga ctccgaccac gaattccatt ggcgtgtagt
      841 cgtcgttcaa cagaaccacc tggtacatgg gcggcggctt gactcgagaa gtctgcttct
      901 cgacgaccgt gccttcgtca atccgctcac tcattgaccc tcattctatg caaaccggac
      961 ccacttacct gtatacaaaa agcgtaccaa tcatacggta aatcattctg tgccccgctg
     1021 aatttccgac gggtgcacag ggcagagacg catgaagcat cagaggtgaa acacaacttt
     1081 cggttccttg aacgtattga atatcgacac tgtgaacaac cgcgcccgaa gcaaccggag
     1141 cgacggttgt aatgccaaaa acgcggcggc ggttgtcttc ggagaccgct tgaccgccgg
     1201 ggcttttgct cgaacaatcc cgccatcaag ccggtatgcg caggcatgcg gggcggatgc
     1261 gcacccaaat tgaagcggac gtgcgcggaa tcggggcagc gccggccgcc aggatgtcaa
     1321 cggcgccgct ccttcattcg tgggaaggaa gaggcatggc gacaggtacg gtcaagtggt
     1381 tcaacgacgc taaagggtac ggcttcatca caccggacga cgggggcgaa gacttgttcg
     1441 ctcacttctc ggccatacag atgaacggct tcaagaccct gaaagaagga cagaaggtgc
     1501 aattcgaggt cgtgcagggt ccgaaaggca agcaggcgtc gaacatcacg gcacccgcct
     1561 gaagcaaggc acgctccgca tcaaccccgc tgcggcgggg ttttttattg gttcgtcgct
     1621 cgtgtacgcg atcggctctg ccgatcgcac gtgcactcgc tccggttacg ggtcgctctg
     1681 cgagcgatcc gcacaggact gatcagtcgg ctgcgctccg gagcggccgc gcgcttcgcc
     1741 gaaggacaag ggcgggcacc ccaccccgcg atgcaccgag ccggcggttg tttagtcatt
     1801 gccctccccg ccaggcggtg agggagaggg aaggttgtct ggaccctccc gcgtggcggg
     1861 agggcagggt gggtgcaatg aagttgaaat tcggcggagc gcgcggccgc tccggagcgg
     1921 agccgacaaa cttgtcttga gcggctctcg caaagcgaga gccgtaccgc tccgagtgca
     1981 cgtgcgaccg caaagcggtc gcgtacacga ggagcgcagc tttacatatg ttcgatcatc
     2041 acctgcccaa acccggagca gctgacctgg gtggcgcctt ccatcaggcg cgcgaagtcg
     2101 taagtgacct tcttgctgag gatcgaccgt tccattgact tgatgacgag atcggcggcc
     2161 tcggtccagc ccatgtgacg cagcatcatc tcggccgaca ggatctcgga acccggattg
     2221 acgtaatcct taccggcata ttttggggcg gtgccgtgcg tggcttcgaa catcgcgacc
     2281 gaatccgaca ggttcgcgcc cggtgcaatg ccgatgccac cgacctgtgc ggccagcgcg
     2341 tcggagatgt agtcgccgtt gaggttcatt gttgcgatga cgtcgtactc ggccggccgc
     2401 agcaggatct gctgcaggaa cgcatcggca atgacgtcct tgagcacgat gtcgccgtgc
     2461 ggcgccttga tcttcatcca cggaccgccg tcgagcagct cggccttgaa ttcgcgctgt
     2521 gccagctcgt agccccattt cgcgaaggcg ccttcggtga acttcatgat gttgcccttg
     2581 tgcacgaagg tcaggctctt gcgcttgttg tcgatgcagt actggagcgc cttgcgcacg
     2641 agccgctcgg tgccttcgcg cgagatgggc ttgatgccga tgccggaggt cgccgggaaa
     2701 cggatcttct tgactcccat ctcgtcctgc aggaacttga tgagcttctt cgccttgtcg
     2761 ctttccgcct cgtactcgat gccggcgtag atgtcctccg agttctcgcg gaagatcacc
     2821 atgtgagtct tttcgggttc cttgaccggg ctcggcacgc ccttgaagta ctggatcgga
     2881 cgcaggcaga cgtaaagatc gagttcctgg cgcagcgcca cgttgatcga ccggatgcca
     2941 ccgccgaccg gcgtcgtcaa cggccccttg atcgacacga cgtagtcgcg cagcacggtc
     3001 agcgtttcct ccggcagcca gacgtccggg ccgtacacct tggtcgactt ctcgccggca
     3061 tagatctcca tccagacgat cttcttcttg ccaccatagg ccttcgcgac cgcagcatcg
     3121 accaccttga tcatcaccgg cgtgatgtcc acgccagtgc cgtcaccctc gatgtagggg
     3181 atgatcggct ggtcgggaac gctgaggctg tagtccttgt tgacggtgat cttctggccg
     3241 gtcgtgggga ccttgatgtg cgcgtacatg gcgtctccgg gcggtgtctt cttgtgaatg
     3301 gcaacggctc ggcaacgttc ttcggccggc cgattgcctc gatagcaggg tcgagagttt
     3361 actcgggccg actgtcgctg gacggtcctg cccatgacgc gtgtcaaccg cacgccttga
     3421 ttgcccgtta aaaaaccgac cactcggtcg tccacacaga gggacggcgc gcggacacag
     3481 cgtctgcggc cacctgctgt acctagggag aaagtaatga agaaagttat tgccgcgctg
     3541 atcagcgccc tgtttgccgg cgctgtgctg gcccaagccg ctgctccggc cgctccggcc
     3601 aaggcggcca ctccggcaac tccggcgagt ccggcaacac cggccgcacc ttcggcagcg
     3661 cctgccactc cggccacacc ggcggccgct ccggcggcca aggcggacgc caaggccacg
     3721 accgacaaga aggccaagtc caccaagaag accaccacca gcaagaagac gatgaccgac
     3781 aagaaggcca cgacggatgc gaagccgaag gctgacgcgg cggcaaccga caagccgatg
     3841 aagtaagccc tgcgtggccg gagaaaggca gggagacccc tgcctttttt tcgtccgtta
     3901 cacggcgcct gagatccgac gtcggcgcgg taaagtcacc ccagccatcc gactcttgaa
     3961 ttgcccgatg tccgatccat ccacattgct acgtcgtttc tacacgctcc tgctcagcct
     4021 ggcgctggca ggcagcgcct acgcccagtc ggggccgcaa ccgaccctgc cgacggtgcg
     4081 cctcagcgcc ggcatgcacg tcatcaccgc tgaactggcc accacgccgc agagccgcac
     4141 catcggaatg atgttccgcg aaaaggcggc gccgaatcac ggcatgcttt tcgtcttcga
     4201 gtacaaggca cagcagtgct tctggatgcg caatacaccg ttgccgctgt cgatagcctt
     4261 catcgacgaa gacgggacga tcctgcaaat cagcgacatg gcgccgaaga gcgaagcgtt
     4321 gcactgttca cagcgtccgg tgcgctttgc gctcgagatg gaacagggat ggttcgccag
     4381 gaagggcgcg ggcgttggcg cgaaaatcgg cggcctgccc gggacccgct agcgcgtcgt
     4441 cgaagcgacg gccgccactg cggccgccgt tcgcggatac agcggtcttt agccgaagtt
     4501 acgcgccgcg aagtcccagt tgacgagcga cgcgaggaag gcctcgacga acttcggtcg
     4561 cgcattgcgg tagtcgatgt agtaggcgtg ttcccacacg tctaccgtca gcagcgcctt
     4621 gtcggccgtc gtcagcggtg tcgccgcatt gctcgtattg acgatgtcaa cgctgccgtc
     4681 tgccctcttc acgagccagg tccagccgga gccgaagttg ccgaccgcgc tcttcgcgaa
     4741 ggcctctttg aacgccccgt aggagcccca cttcgccgcg atcgcctgcg ccagtgcgcc
     4801 gctcggctcg ccaccgccgc ccggcttcat gcagttccag aagaagctgt gattccacac
     4861 ctgggccgca ttgttgaaca caccgcctga cgccttgcgc acgatgtcct cgagcgacaa
     4921 cgactcgaac tcggtgccct tgatcaggtt gtttaggttg gtgacatagg cctggtggtg
     4981 cttgccgtag tggtattcga aggtctcctt cgacatgtgc ggcgccagag cgtcggtggc
     5041 gtacggaagt gcgggtagcg tgtgttccat gctttacctc gggggaatgt cgcggtggaa
     5101 aagtgcggtg gagaaaggag attctatcgt cgcaagctca atgcccgccc gcgacgccgt
     5161 cagggccatc gccttgcagc gactcgaccc gcactccggc cgcaccttcg gcgagcacca
     5221 gccgcagccg cgcacccacc gcgaggtcct gcgcccgcgt caccgcacgg ccgctgccgt
     5281 ccagcgcgat cgcatacccc cggcgcaaga ctgccgtggg gtcgagcgtc gccagccgcg
     5341 ccatcaacgg acccagccgc atcgcgttgt cattgagcag cgccctgccg agcgccacga
     5401 gccggttcct ctgccccagc agtccttcct gatcacgact cggacgaacc tgcagccagc
     5461 gcgcgtgctg tgcctgcagg cgtgcggcgg cccgagccgt cgcctgcacg atggcccctg
     5521 ctgcgcgtga ccggagcagc gcgatacgcg catcgtaggc agcgagcggc gcacgcggcg
     5581 ttgcgaggct gcgctgcgcg aagtcgatgc gctgtgatgc ggcctgcaat cgcgtctgca
     5641 actggcgcgc cagatgccgc atctgattgg cgagcaactc ctgcagcgcg tccgcactgg
     5701 tggcgaccag ttccgctgcc gcggtaggcg tcggagcacg gacgtcggcg gcaaagtcgg
     5761 cgatcgtgac atcgctttca tggccgacac ccacgaccac cggcagcacg caggcgcgga
     5821 tcgcccgggc cacggcctct tcattgaacg cccacagatc ctcgatcgag ccgccgccgc
     5881 gcaccagcag cacgacgtcg acgtcgcggc ggccggagac ccgcgccagc gtcgccgcga
     5941 tctcggcgcc ggcgccggca ccctgaaccg gtaccggata gacgatgacg cccacgtacg
     6001 gcgcgcgccg ccccagcgcc gtgatcacat cgcgcagagc cgcggcctgc agcgacgtaa
     6061 caactcccac acacatcggc agtcgcggca gcgttcgctt gcgcgccaga tcgaacagac
     6121 cctcggccgc gaggcgctgc ttgagccgca aaaactcctc gtacaggcgt ccgaggcccg
     6181 ctcgccgcat gaactcgacg ttcagctgga attcaccgcg cgcctcgtac agggaaacga
     6241 tcgacgtggc ctcgacttcg tcgccttcac gcggcgccca atccagcaag ctgttgcgtc
     6301 cgcggaacat cacacaccgt acttgcgcga tgtcgtcctt cagcgtgaag taccagtgac
     6361 cgcttgcagc gcgggtcagg ttgcccacct cgccctgcac gcggaccagc gggaagctac
     6421 gttctagcag ccccgcaacg gcgcggttta gcgcggacac cgtcagcgga gaccgcgcca
     6481 aacctgaatc ggccagttcg gaatcaagcg ccatgcggct ttccgggcaa aaactgtcca
     6541 caaacgctga tgtgacgggg ctgtcgccct ccagactctc gtgcaaacgg cttaatccac
     6601 ttcaagtgct tgatttcggg actgttttcc atctgctcaa tcggtgtgca acgaaaacaa
     6661 tgcccgctgt gacaagggct tagcgctcgc cccacgaagt tgttcacata cttgtccaca
     6721 ggaactgtgg aaacatgcgg cccgaggggc aaacttgcgg ccacctgagc gcaccactac
     6781 gcttcgcgct tttcgcgtgc cgcgcgggag tcaacgcact tgcttgccat catcatcgaa
     6841 ctgggctggc ccgtctggcc cctcatcctc gcgtccgtgc tcgcgctggc gctgatcatc
     6901 gaacgaacct tcgcgctgcg gcgcattcgt atcttaccca gcggactgtt cgaggacgtg
     6961 gtcggactgg tgaagaagca gaccgtcacg ccggagatcc tggtcaagct cgagcacaac
     7021 tcgccgctcg gccgggtcct gtcggccggc ctgcgcaaca gccgcgtgct gcgcgaaggc
     7081 gcgatgcagc agatcgaggc ggcaggcgcg gtcgtggcga accggctcac caagaacctg
     7141 accctgctgg gatcgattgg atcaatcgcc ccgctgctgg gattgttcgg aaccgtggtc
     7201 gggatgatcc agatctttgc gtcgcagcag gccgggtcga gtccgcagga actggcccgc
     7261 ggcatttcgg tggcgctcta ctgcaccgcc ttcggcatca tcgtcgcggt accggcggta
     7321 ctcgcccacc gctacttccg ccgtctggtc
//
LOCUS       DATIRT010000177         6677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_811665, whole genome shotgun sequence.
ACCESSION   DATIRT010000177 DATIRT010000000
VERSION     DATIRT010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6677
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811665"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..2110)
                     /locus_tag="VLN25_04220"
     CDS             complement(230..2110)
                     /locus_tag="VLN25_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HSQ09809.1"
                     /translation="MPAASTTYPHRDALLAHQLKALFPDVGPETLAFMQQHLEWVEIA
                     AGETLISQGAPGDSAYLTISGRLRVYVAADDLPRMVRELSRGEVIGEMSLYTGEPRSA
                     TVVAVRDSVLVRLAKEHFEALLVSHPQVSLTFTRQMIRRLQTEHERRPMAAPVTVGLL
                     PVSAGVAIGDFAQRLAAPLAQFGRVVVVDSAEMDRLLSEQGITARSDAEADHRISLAL
                     DGLEAGHDFVLLLADAFPGPWTHRCVRNSDEMLLVAEAAQTPAVHEIEKTCLSGRPAR
                     SEAAEILVLLHPSESRAPRGARAWLQRRPVTGHVNIRPDLDRDMARLARLLSRNAVGL
                     VLAGGGARGFAHLGVWRALQERGIEIDVVGGTSIGAIMACLIAIDRPIDKAIDVARRA
                     FSLNPTGDYNLLPLVSLIKGRRVRTAIRRSIEELVGGPVDIEDLWKGYFCIASNYSQA
                     REQRIDGGDLEHALLASAAIPGALPPVIRDGDLLCDGGTFNNFPVDAMRDLRGVGKVI
                     GVDLGVRSARRLEFDEVPGSWTMLLDRLRPRRQRRFRLPSLTAYLLNITILYSTSRQR
                     EVQRLTDLYFNPPLYRVGLLQWSRFDQIVRQGYEYAIGVLDGEPPTRRDTPGVAPARA
                     TG"
     gene            complement(2192..4465)
                     /locus_tag="VLN25_04225"
     CDS             complement(2192..4465)
                     /locus_tag="VLN25_04225"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HSQ09810.1"
                     /translation="MSETVAPSNWWSSLFQSRGIARGDIAGGVTAALVLPAIEGSYGL
                     LAFAGLGADKAAIGFLLGACTAAIACIVTMVAGGRGPMLSGSSAALALLLASLIGWLS
                     ADPRFLGADGLPVLPLLLAFTALGVVLAGVMQVMLAKLKLGGLVRYVPYPVHAGYMNG
                     VAVVMVGGMLPHLLGLPLGATAMDWHQMNVLAPVIALVALWLALNAPVWTRRIPPYLT
                     GLLAATGLHHLLSFTPLAGGLGPLFEAPQFEWPGLNVMAPVAAHFNSGLLRDMIGPLL
                     LFAAAVAMMSTLQTALAGSTIDELTRTRRNGDRELLAQGMANIAVGVIGALPTAGSTT
                     RSKINLDAGGTTAMSRVVFGVALLVALAFGLQYMNLLPMAAIAGVFCAVAFSLVDDWT
                     RRATGVLWRQTLKWRAPMALLQSYLVMLLVAGVTIFVSLALAIALGTLVAMILFIRSN
                     CKKPIRQVVHGDRRRSRKVRPGEQTEHLHAEGKRIAVIELDGALFFGTAEEADEEIEH
                     LAHASEFIIIDFGRVTDVDASGARVLLHAASTVQRAGKHLLFAGLSLTDSRTRIIRDM
                     DVHDRLADAQFFPDVDRALEYAEDQLLASLAVASNAAGATLRLEQTLLGARLDPDEIA
                     VLAPMLKERRCSKGEVVFRRGDPGDAMYVSLRGSIGIWLPADPAGGGGAGRRMVSYAL
                     GVVFGEMGLLQGLSRSADAIAEDEAVVLELSRANYERLGTEYPVLHGKLLLSFGLLLS
                     ARVRALTDELETAQTVR"
     gene            complement(4571..5368)
                     /locus_tag="VLN25_04230"
     CDS             complement(4571..5368)
                     /locus_tag="VLN25_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018915155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HSQ09811.1"
                     /translation="MLLCQISDPHVVKEGTLAYGRIDTPRMLERCVSKILALPRRPDA
                     VVATGDLTDHATADEYAALRKLLAPLAAQMPVYLAVGNHDDRDALRTAFPEHPYLAGH
                     DGFIQYVVEDFPVRLVVLDTLIPGAPGGELCAERLRWLDRTLAASDRPTIVAQHHPPF
                     VTGMAMMDQMTLANPDAEAAVIGKYRHVERVICGHYHRNIQARFAETVASVCPSSAHQ
                     LLLDLVPDAGIRFTFEPSSFHLHLWNGRQLVTHTELVEDFPTWGSHG"
     gene            complement(5596..6003)
                     /locus_tag="VLN25_04235"
     CDS             complement(5596..6003)
                     /locus_tag="VLN25_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09812.1"
                     /translation="MTLVRTGAFLATAVLAAMSAGCGKKEPPAVAPAARTQPPPVIPA
                     PPKPPPSYLRDHYAKLEDCVYDWGYAQKCTPVAPGSATQQAGAHFLGPIYAKSYREET
                     QAQLRKEAIDGGYAQRVAADASDSSISKSEVKP"
     gene            complement(6093..6377)
                     /locus_tag="VLN25_04240"
     CDS             complement(6093..6377)
                     /locus_tag="VLN25_04240"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HSQ09813.1"
                     /translation="MPAFIVGTIRVTDTAAWQRYVDRVGATFGPHGGRVVLRGAKVSD
                     LARQAHGERVVVAEFADIDALMRWHDSAEYQALVSLREAGAEVVLTAYQT"
     gene            complement(6377..>6677)
                     /locus_tag="VLN25_04245"
     CDS             complement(6377..>6677)
                     /locus_tag="VLN25_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09814.1"
                     /translation="AADGTALFDLVVIENSAEHVVYRGIDRAHVERVRSALADGSIDV
                     YAVQRVLTEVVLRRGGAPRSMFVRGMPIPLPGTSGTPKDRWTEARAQMEALLEGH"
BASE COUNT         1212 a         2284 c         2150 g         1031 t
ORIGIN      
        1 tccttcagga tcttgaacgg caggcggagc gcctccggcg tgtcattcca gccggcatcg
       61 tcaatcaggg gtttgccata accctcggcc cggctcaatt cgccggaagc ggcggcctcc
      121 gccaggcggc gggcaatctc ttcgtccagg ctgcgcatct gggtactccg gatcgtgagc
      181 acggggcgat tgaagcacag gtcctgcgcc ctggcgatgg caatccgagc tacccggttg
      241 cgcgtgcagg agcgacgccc ggcgtgtccc gccgtgtcgg aggttcgccg tcgagcacac
      301 ctatcgcgta ctcgtacccc tgccgcacga tctggtcgaa gcgcgaccac tgcagcagcc
      361 cgacccggta cagcggcgga ttgaagtaca ggtcggtgag ccgctgcact tcgcgctggc
      421 gcgaggtgct gtaaagaatc gtgatgttga gcaggtaggc cgtcagcgac ggcagtcgga
      481 aacgccgctg gcgacgcggg cgaaggcggt cgagcagcat cgtccagctg cccggaacct
      541 catcgaactc cagacgcctc gcactcctca cgcccaggtc gacgccgatc accttgccga
      601 cgccgcgcaa atcccgcatc gcatcgaccg ggaaattgtt gaacgtgccg ccatcgcaca
      661 gcaggtcacc gtcacgaatg acgggcggca aggctccggg gatcgcggcg cttgcgagca
      721 gcgcgtgctc gagatcgcca ccgtcgatgc gctgctcgcg cgcctgcgaa tagttgctgg
      781 cgatgcagaa gtagcccttc cacagatcct cgatgtcgac cggcccgccg accagttcct
      841 cgatcgagcg ccggattgcc gtgcgcaccc gacgcccctt gatgagcgac accagcggca
      901 gcaggttgta atccccggtc gggttcaggc tgaaagcccg gcgcgcgacg tcgatcgcct
      961 tgtcgatcgg ccgatcgatc gcaatcaggc acgccatgat cgcgccgatg ctggtcccgc
     1021 cgacgacgtc gatttcaatg ccgcgctcct gcaaggcgcg ccagacgcca agatgagcaa
     1081 agccgcgcgc gccgccgccc gccaggacca gccccaccgc attgcggctc aacaaccgcg
     1141 ccagacgcgc catgtcgcga tcgagatcgg ggcggatgtt gacgtgcccc gtcactggtc
     1201 ggcgctgcag ccaggcccgg gcgccgcgcg gggctcggct ttcggaagga tgcaggagca
     1261 cgagaatctc ggcggcctcg ctgcgcgccg ggcgaccact gaggcaggtc ttctcgatct
     1321 cgtgaacggc gggcgtctgc gccgcctcgg caaccagcaa catctcgtca ctgttgcgga
     1381 cacagcgatg cgtccacggt ccagggaatg catcggccag cagcagaacg aagtcgtgtc
     1441 cggcctcgag gccgtcgagt gcaagcgaga tgcggtgatc cgcctcggca tccgagcgcg
     1501 cggtgatgcc ctgctcacta agcaggcgat ccatctcggc gctgtccacc acgacaacgc
     1561 ggccgaactg cgccagcggc gcggccaggc gctgcgcaaa gtcgccgatt gcgacgccgg
     1621 cggaaaccgg caacaggccc accgtgaccg gcgcggccat tgggcggcgc tcgtgctcgg
     1681 tctgcagtcg ccggatcatc tgacgcgtga acgtgagcga gacctgcggg tggctgacca
     1741 gcagcgcttc gaagtgctcc ttcgcgagtc gcaccagcac ggagtcacgc acggccacca
     1801 ccgtggccga gcgcggctcg ccggtataca ggctcatctc gccaatgact tcgccgcgcg
     1861 aaagttcgcg caccatgcgt ggaaggtcgt cggccgccac gtagacgcgc agccgtccac
     1921 tgattgtcag gtaggccgag tcgcccggcg caccctgcga aatcagcgtc tcgccggccg
     1981 cgatctcgac ccactccaag tgctgctgca tgaacgccag cgtttccggg ccgacgtccg
     2041 gaaacagcgc tttcagctga tgtgcaagca gtgcgtcgcg atgcggatac gtcgttgatg
     2101 cggcaggcat gcgtgcaagt gttgccggtt tccgaccgtc ggtccagcat gcggcacggc
     2161 cgggtcgcgc cgcatgcgga ttgtcttgtg ctcagcgcac cgtctgcgcc gtttcgagtt
     2221 cgtcggtcaa cgcgcgcacg cgggccgaca gcagcaggcc gaagctcagc agcagcttgc
     2281 cgtgcagcac cgggtattcg gtgccgagcc gctcgtagtt ggcgcgcgaa agctcgagca
     2341 ccacggcctc gtcctccgcg attgcatcag cggagcgcga caggccctgc agcagtccca
     2401 tttcaccgaa gacgacaccc agagcgtagg acaccatgcg gcgacccgcg ccgccaccgc
     2461 ccgcaggatc ggcaggcagc cagatgccga tggatccccg cagcgagaca tacattgcat
     2521 caccgggatc gccgcgccgg aacaccactt cccccttgct gcaacgccgt tccttcagca
     2581 taggcgccaa cactgcgatt tcatccggat cgaggcgcgc cccgagcaac gtctgctcca
     2641 gccgcagggt cgcgccggca gcgttcgacg cgacggcgag cgacgccagc agttgatcct
     2701 cggcatactc gagcgcccga tcgacatccg ggaagaactg ggcgtccgcg aggcggtcat
     2761 gcacatccat gtcgcggatg atgcgcgtac gcgagtctgt cagcgacagg ccggcgaaca
     2821 acaggtgctt gcccgcccgc tgcaccgtcg aggccgcgtg cagcagcacg cgagcaccgc
     2881 tggcgtcgac gtcggtgaca cggccgaagt cgatgatgat gaactcggat gcatgggcaa
     2941 ggtgttcgat ctcctcgtcc gcctcctccg cggtaccgaa gaagagcgcg ccgtccagct
     3001 cgatcacggc aatgcgcttg ccttcagcgt gcaggtgctc agtctgttcg cccggacgca
     3061 ccttgcgcga acggcggcga tcgccgtgca cgacctggcg gatcggcttc ttgcagttgc
     3121 tgcggatgaa gagaatcatc gcgaccagcg tgcccagcgc aatcgcgagc gcgagcgaca
     3181 cgaaaatggt gacgccggcc accagcagca tgaccagata gctctgcagc agcgccatcg
     3241 gcgcgcgcca cttcagcgtc tggcgccaca gcacaccggt ggcgcggcgg gtccagtcgt
     3301 ccaccagcga gaacgccact gcgcagaaca ccccggcgat ggcagccatc ggcaacagat
     3361 tcatgtactg gagcccgaac gcgagcgcca ccagcagtgc gacgccaaag acgacgcgcg
     3421 acattgcggt cgtgccgccg gcgtcaaggt tgatcttgct gcgtgtcgtc gatcctgccg
     3481 tcggcagcgc cccgatcacg ccgacagcga tgttggccat gccctgcgcc agcagttccc
     3541 gatcaccgtt gcgtcgcgtg cgcgtcaact cgtcgatcgt cgatccggcc agtgcggtct
     3601 gcaacgtcga catcattgcc actgccgccg cgaacagcag caaggggccg atcatgtcgc
     3661 gaagcaaccc gctgttgaag tgagcggcca ccggtgccat cacgttgagt cccggccact
     3721 cgaactgcgg cgcttcgaag agcgggccaa gcccgccggc gagcggagtg aacgacagca
     3781 gatggtgcaa tccggtcgcg gcgagcaggc ccgtgaggta cggcggaatg cgccgggtcc
     3841 agaccggggc gttgagcgcc agccatagcg ccacgagggc aatcaccggt gccagcacgt
     3901 tcatctggtg ccagtccatc gcagtcgccc ccaatggcag gccgagcagg tgcggcagca
     3961 tcccgccgac catgacgacg gccacaccgt tcatgtagcc ggcgtgcacc ggatacggaa
     4021 catagcgaac gagtcccccg agcttcagct tggccagcat gacctgcatc accccggcca
     4081 gcacgacgcc gagcgcggta aaggcgagca ataacggcag gacggggaga ccgtccgcgc
     4141 cgagaaaacg cggatcggca ctgagccaac cgatcaggga cgccagcagc aatgccagcg
     4201 cggcactcga gccactcagc atcgggcctc gcccaccggc gaccatggtc acgatgcagg
     4261 cgatggccgc tgtgcacgcc cccagcagga agccgatggc cgccttgtcg gcgcccaggc
     4321 cagcgaacgc cagcagcccg tagctgcctt cgatcgcggg cagtaccagc gccgcggtga
     4381 cgccgcccgc gatgtcgcca cgggcgatgc ctcgactctg gaaaagtgaa ctccaccaat
     4441 tcgatggcgc gaccgtttcg ctcatgcagc ccccaacacc aggcgagtct gcaccgaccg
     4501 atctgacgcg tttcctcctg cggcagcgaa accgcgccgc ccgagcgtcg accgggtgcg
     4561 aatcacggcg tcagccatgc gagccccagg tgggaaagtc ctcgaccagc tccgtgtgcg
     4621 tcacgagctg ccgaccgttc cacaaatgca gatgaaagga cgacggctcg aaggtgaagc
     4681 ggataccggc gtccggcacg agatcgagca gcaactgatg cgctgaactc ggacacacgg
     4741 aggcaacggt ttctgcaaag cgggcctgga tgttgcgatg gtagtgaccg cagatcacac
     4801 gttcgacgtg ccggtacttg ccgatcaccg ccgcttcggc gtccggattg gccagagtca
     4861 tctgatccat catcgccatg ccggtcacga acggcggatg gtgctgcgcg acgatcgtcg
     4921 gccgatcgga cgccgccagc gtgcgatcga gccagcgcag acgctcggcg caaagttcgc
     4981 cgcccggtgc gccaggtatc agcgtgtcga gcacgacgag acgcaccggg aagtcttcga
     5041 cgacatactg gatgaatccg tcgtgccctg ccagataggg gtgctcggga aatgccgtcc
     5101 gcagcgcatc gcggtcatca tgattgccga cggcgaggta caccggcatt tgcgcggcca
     5161 gtggcgcgag cagcttgcgc agcgccgcgt actcgtcggc cgtcgcatgg tcggtcaggt
     5221 cgccggttgc caccacggcg tctggccggc gcggcaacgc caggatcttg ctgacgcaac
     5281 gctcgagcat gcgcggcgta tcgatgcgcc cgtacgccag cgtgccttcc ttgacgacat
     5341 gcgggtcgct gatctggcag agaagcatgc gtgagttcca ggttgaactg aggtgtcgtt
     5401 acgggacgag gcagtccggg acgatgcacc cggtccgacg ggccagccct tcccggcccc
     5461 ttggcggcgc cgacaaagcc cgcagcgtag tcgaagccgg ggagcacggc gcgtggaccc
     5521 ggtccggcgg catggcgtcg cgcactgtca gccgcgttgc ggcgccaact cggcgcgact
     5581 tgctccccga tcccctcagg gcttcacttc agacttgctg atcgaactgt cactggcgtc
     5641 ggccgcgacg cgttgggcgt agccgccgtc gatcgcttcc ttgcgcagtt gcgcctgcgt
     5701 ttcttctcta tagctcttgg cgtagatcgg gcccaggaaa tgggcacccg cctgctgggt
     5761 agcagaaccg ggcgccaccg gcgtgcactt ctgcgcatag ccccagtcgt agacgcagtc
     5821 ttccagcttc gcgtagtggt cacgcaggta cgacggcggc ggcttggggg gcgccggaat
     5881 caccggcggc ggctgggtcc tggcggcggg tgccacggcc ggcggttcct tcttgccgca
     5941 tccggcagac attgctgcca gtacggccgt cgcaaggaat gcgccagtac gaaccaaagt
     6001 catgtcagga gtctctccga agtagtcgca gggtcaatag gcttgcagcg gagtcgcgca
     6061 agtgcggtcc ggcagtatcg ccattcaagg cgtcatgtct ggtaagcagt gaggacaacc
     6121 tcggctcctg cttcgcgcaa cgaaaccagc gcctgatact cggccgaatc atgccagcgc
     6181 atcagcgcgt cgatatccgc aaactcggcg acgacgaccc gctcgccatg cgcctgtcgc
     6241 gcgagatcgc tcactttcgc accgcgcagt accacgcgcc cgccgtgcgg gccgaacgtg
     6301 gcaccgaccc ggtccacata gcgctgccac gccgccgtgt cggtgacgcg aatcgtcccg
     6361 acgatgaacg ccggcatcag tgaccctcca gcaacgcttc catctgcgcg cgtgcttccg
     6421 tccaacggtc tttgggcgtt ccagacgtgc cgggcagtgg aatcggcatg ccacgcacga
     6481 acatcgaacg aggcgcgcca ccacgacgaa gtactacctc cgtcagcacc ctttgcaccg
     6541 cgtagacatc aatcgatcca tccgccaagg cactccggac gcgctcgaca tgggcacggt
     6601 cgatgccgcg atagacaacg tgttcggccg aattttctat tacgacaaga tcgaacagcg
     6661 cagtgccgtc tgcggcc
//
LOCUS       DATIRT010000178         9246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_418740, whole genome shotgun sequence.
ACCESSION   DATIRT010000178 DATIRT010000000
VERSION     DATIRT010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9246
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418740"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..130)
                     /locus_tag="VLN25_04250"
     CDS             complement(<1..130)
                     /locus_tag="VLN25_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013337902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HSQ09815.1"
                     /translation="MPQLTTANTGPLLALEQKIVDEMPAIERWFRLQWQDHTPPFYG"
     gene            complement(454..2031)
                     /gene="amt"
                     /locus_tag="VLN25_04255"
     CDS             complement(454..2031)
                     /gene="amt"
                     /locus_tag="VLN25_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HSQ09816.1"
                     /translation="MKKILVMLLTAGAMVLASGGAVAQGGGGAYGDASAPKMEGKAAE
                     AAPTAAPAAAKMEEKSTTVTETVKVTETVKVEEKVEEVAVAPKIEKADTAFMYVATIM
                     VILMTIPGLALFYGGLVRSKNMLSVLMQVFVIFSLISVLWVIYGYTLAFGGPGACFGN
                     FSKLFLSGVNAEAAAATFSKGVYIPELLFVAFQCTFAAITCALIVGSYAERMKFSAVL
                     LFTVIWFTFSYLPMAHMVWYWDGPDAIKDQATLDAVMANAGWLWAKGALDFAGGTVVH
                     INAGVAGLVAAYVLGKRIGYGKESMAPHSLTLTMVGAALLWVGWFGFNAGSNLEANGV
                     AAFAFLNTLLATAVAVLAWMFAEWMVKGKPSMLGAASDAVAGLVAITPACGFVGVIGA
                     MVIGIAAGLLCFWGVTGLKKMLGADDALDVFGVHGVGGIVGALLTGVFAAPSLGGAGI
                     CNYITNKCGDPSDFPGIGAQLWIQFQAVLTAVILSAVVSLVALYIVKMITGLRVTPEE
                     EREGLDTTYHGESAYKM"
     gene            complement(2047..2385)
                     /locus_tag="VLN25_04260"
     CDS             complement(2047..2385)
                     /locus_tag="VLN25_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HSQ09817.1"
                     /translation="MKLISAIIKPFKLDEVREALSGVGVQGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEAAVADDIVERAIEAIEGSARTGKIGDGKIFVYDLEQVVRI
                     RTGETGKDAL"
     gene            complement(2419..3207)
                     /locus_tag="VLN25_04265"
     CDS             complement(2419..3207)
                     /locus_tag="VLN25_04265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HSQ09818.1"
                     /translation="MKKAFIAASVAAACSAPLLAAAQTAAPAATPDHTFSGNLTIASD
                     YRFRGFSQTDYQPAIQGGIDYSHSSGFYLGNWNSNISSTLYNGAPIEMDFYGGWKTSF
                     DGGFGLDVGAIYYYYPGTGEYNPDFEAKNFEAYIGGSWGPASVKYYYAFTDFFGVKAP
                     GYETKGSQYVDFSATFPLADGFAIVAHAGWQFVKNYENINTALNKDNVWDFKLGGTWD
                     IMGSGWILGAFWIGTSEKDFFTTAEVPSRGAGRNSLVATVGKTF"
     gene            3474..3710
                     /locus_tag="VLN25_04270"
     CDS             3474..3710
                     /locus_tag="VLN25_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HSQ09819.1"
                     /translation="MDKISFLDDLQSRVSSLIQQTPAADLQKNLKALLTQQFARLELV
                     TREEFDTQSKVLARTRAKLDELERRLAELEQRKT"
     gene            complement(3780..4688)
                     /locus_tag="VLN25_04275"
     CDS             complement(3780..4688)
                     /locus_tag="VLN25_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09820.1"
                     /translation="MSSLLFGIGLLVVLVGLYLWARRRAEAIRLQAAQREQAAMAMML
                     GSSAPEAKAKASKANPAAPVEEIDHAALIRQAHGEAAEQDHASKDLPPIEYDHKRPGP
                     SRNMTPAEEAAAGLDPIEELISQIMVDDSGRPAAPARVSAPTPPEPKRANAASRAAAR
                     EQTPMLDSMAPSVPLRELVLAWYESRGYRQVPASPAVWPIELVLRHRDDPSRAYAFVV
                     QNDHVSVDRITALIEQAREIGMLRVAVIAEAGYERGAKEIARRRNVRLIDRPTMDAEL
                     SDLQLETAAKIIAVARKRASPEASAA"
     gene            4944..6479
                     /locus_tag="VLN25_04280"
     CDS             4944..6479
                     /locus_tag="VLN25_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HSQ09821.1"
                     /translation="MSIAIVRTRGLSGMYAPQVTVEVHLANGLPSFTLVGLPDTEVRE
                     ARERVRAALLTSRFDFPTRRITVNLAPADLPKESGRFDLPIALGILAATGQIPTRTLD
                     QYEFAGELSLTGELRPVRGTLAMAYAVAKSEHTVGPRRGFVVPFESADEAALVGGIPI
                     FPARTLLQVVEHLRADAGDTSARAIAEHQAMVRLTPPIYPDLFDVKGQLGAKRALEIA
                     ATGGHSVLLVGPPGTGKTMLAQRFAGILPPMTTDEALESAAVLSLVGQFAPQRWATRI
                     FRSPHHTASAVALTGGGSPPMPGEISLAHHGVLFLDELPEFERRVLETLRQPFESGTI
                     TVSRAARQVDFPSRFQLIGAMNPCPCGYYGHASIACRCTPDMVARYQDRISGPLLDRI
                     DMRVEVSSVPEQVMTSLPDGEPTRFVAERVRKGYDRAIARQGKVNDHLQGAELEEVCS
                     FDEAVQKLLHAASVRLGWSARAYHRVLKVARTIADLADADGLAPHHVAEAIQYRRSLR
                     DTS"
     gene            complement(6501..8747)
                     /locus_tag="VLN25_04285"
     CDS             complement(6501..8747)
                     /locus_tag="VLN25_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HSQ09822.1"
                     /translation="MTLRLRARAIAIASVLLILSGASAQTSPTSPAETPAAKQRPKVG
                     LVLSGGGARGAAHIGVLKVLEELRVPVDVIAGTSMGSIVGAAYATGLSVPEMEAAVAK
                     ITTATLFTDKPPRADQSMRQKTDDQLPYLIPELGIGKDGVALPKGIVTGVALEGELRK
                     LVQIANVRSFDELPIPFRAIATDIGTGEMVVLKEGSVVAAIRASMSVPGAVAPVTIGK
                     RQLVDGGLTNNLPIDIARSMGADVIIAVNLGTPLLRPDEITSVLSVTLQMINILTEQN
                     VGRSLKDLRAGDVLIVPELGNFSSADFDNLMTTVPIGEEAAWKVADRLRALSLPPEQY
                     AALRSKQAAPTASAPVIVEAIKVEGTARVSPVVVLQSLRTQVGDPIERDRIDLDMRRV
                     YGRGDFENVNYTVEEIDGKQTLVVLVKEKPERHYFRFGLELDADLGKDANFNLYASHR
                     AKWLNAWGAEWRNDVVLGTNVLLGTEFYQPLGPRQYFFIAPQLRYAIDPWYLFSDDVR
                     IAEYQDTSWVAQFDVGANIIEYGEVRLGAAYGRRTFELQSGGFLFPSRLATSIGAIEG
                     VARFDRLDSVNFPHRGYFASAGFYSSLSALGADDEYTKWEARLNGAATWGRHTVEGLV
                     TAGGKVGSNDIAIYDQFNLGGFLYLSGLQRQQIKTQDFTFGRLVYRTKMGDIPFFEGV
                     YLGASAEAARTKLLIPVWNGEPADGYVNVLAGSVFLGVDSPLGPLYVGFGYSSKENRA
                     VYLFLGRP"
     gene            complement(8889..>9246)
                     /locus_tag="VLN25_04290"
     CDS             complement(8889..>9246)
                     /locus_tag="VLN25_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004200059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSQ09823.1"
                     /translation="EEIFGPVLTMVHVPDFAAALQLVNGHDLGNGVACYTTDGGTARE
                     FARRVDAGMVGINVPIPVPMAFHSFGGWKRSLFGDHHAYGEEGVRFYTRYKSVMQRWP
                     DASASKGPEFTMPVNR"
BASE COUNT         1630 a         3079 c         2930 g         1607 t
ORIGIN      
        1 agccgtagaa cggcggggtg tggtcctgcc actgcagccg gaaccagcgc tcgatcgccg
       61 gcatctcgtc gacgatcttc tgctcgagcg ccagcagggg gcccgtgttg gccgtggtca
      121 gctggggaat tgtgttcgac acgacgggct cgcttctgcg cggtccaacc agagtcaatc
      181 ggccgcgcca ggtcgttggg atcggcagtt tagcgccgcc ctactttgat ccccgagtcg
      241 cgttccgggt tgtccggcat gccaggcgaa atgaccaaaa aaaaaacggg caccccagag
      301 ggtgcccgtg aaagttcgcc acgagtgggc tgcggggcag cccggcgagc tagggagaca
      361 caaccttcac ttgcgccgtc gcgccacgtc cggtttggcg ccacggcggc ccgggagtcg
      421 cctcccgggc cctttcgttg actacatcgt tcattacatc ttgtaggcgg actcgccgtg
      481 gtacgtcgtg tcgaggcctt cgcgctcctc ttccggcgtc acacgcagtc cggtgatcat
      541 cttgacgata taaagcgcca ccagcgaaac cacggccgag agtatgaccg cagtcagcac
      601 cgcctggaac tggatccaga gctgtgcgcc gatgcccggg aagtcggacg ggtcaccgca
      661 cttgttggtg atgtagttgc agatgccggc accgccgagc gacggcgcgg cgaacacgcc
      721 cgtcagcagc gcgccgacga tgccgcccac gccgtgcaca ccgaacacgt cgagtgcatc
      781 gtccgcaccc agcatcttct tcaaaccggt cacgccccag aagcagagca ggccagctgc
      841 gatgccgatg accattgcgc cgatgacgcc gacgaagccg caggcaggcg tgatcgccac
      901 caggccggcc acggcatccg acgccgcacc cagcatcgac ggcttgccct tgaccatcca
      961 ctccgcgaac atccacgcca gcaccgcgac agccgtggcc agcagggtat tgaggaacgc
     1021 gaaggccgcg acgccgttgg cttcgaggtt ggagccggca ttgaagccga accagcccac
     1081 ccacagcagt gcggcaccga ccatcgtcag tgtcaggctg tgcggcgcca tcgactcctt
     1141 gccatagccg atgcgcttgc cgagcacgta ggccgccacc aggcccgcaa cacccgcgtt
     1201 gatgtgcacc accgtgccgc cggcgaagtc gagtgcgccc ttggcccata accagccggc
     1261 attcgccatc acggcgtcga gagtggcctg gtccttgatc gcgtccgggc cgtcccagta
     1321 ccacaccatg tgcgccatcg gcaggtagct gaacgtgaac cagatcaccg tgaacagcag
     1381 cacggccgaa aacttcatcc gctccgcgta cgagccgacg atcagcgcgc aggtgatagc
     1441 ggcgaacgtg cactggaacg ccacgaacag cagctcgggg atgtagaccc ccttgctgaa
     1501 ggtcgccgcg gccgcttcgg cgttcacgcc gctcaggaag agcttcgaga agttgccgaa
     1561 gcaggcgccc ggcccgccga aagcgagcgt gtagccgtag atgacccaca gcaccgagat
     1621 cagcgagaag atcacgaaga cctgcatcag cacgctcagc atgttcttcg agcgcaccag
     1681 gccgccgtag aacagcgcca gccccggtat ggtcatcagg atgaccatga tcgtcgcgac
     1741 atacatgaac gccgtgtcgg ccttctcgat cttcggcgcc accgccactt cctcgacttt
     1801 ctcctcgacc ttgacggtct cggtgacctt gaccgtttcg gtcacggtcg tggacttttc
     1861 ctccatcttg gcagcagccg gtgcggcagt cggagcagcc tcagccgcct tgccttccat
     1921 cttgggcgca gacgcatcgc cgtaagcgcc gccgccaccc tgcgcgaccg ctccgcccga
     1981 tgcgagcacc atggctcctg cagtgaggag catgaccaga attttcttca ttgcttcccc
     2041 acgaagttag agagcatctt tgccggtttc accggtgcgg atgcggacga cctgctcgag
     2101 gtcatagacg aagatcttgc cgtcgccgat cttgccggtg cgcgccgagc cttcgatggc
     2161 ctcgatcgcg cgctcgacga tgtcgtcagc cacggcggct tcgatcttca ctttgggcag
     2221 gaagtcgacc acgtattcgg cgccgcgata cagctccgtg tggcccttct gccgtccgaa
     2281 gcccttgact tcggtgactg tgatgccctg aacacccaca ccgctcaggg cctcacgcac
     2341 ctcgtcaagc ttgaacggct tgatgatggc ggagataagt ttcatgaatt cagttcccgt
     2401 tcgttgattc gtgatgcgtc agaacgtctt gccgaccgtg gcgacgaggg agttccggcc
     2461 ggccccgcgc gacgggactt ctgccgtcgt gaagaagtcc ttctcgctgg tgccgatcca
     2521 gaaggcgccc agaatccagc ctgatcccat gatgtcccat gtgccaccga gcttgaagtc
     2581 ccacacgttg tccttgttga gcgcggtgtt gatgttctcg tagttcttca cgaactgcca
     2641 cccggcgtgc gcgacgatgg caaagccgtc cgcgaggggg aaggttgcgg aaaagtcgac
     2701 gtactgagaa cccttggttt cgtatcccgg ggctttcaca ccgaagaagt cggtgaacgc
     2761 gtagtaatac ttcaccgacg ccgggcccca cgacccgccg atgtacgcct cgaagttctt
     2821 cgcttcgaag tccgggttgt attcgcccgt gcccgggtag tagtagtaga tggcgccgac
     2881 atcaaggccg aaccctccat caaacgatgt cttccagcca ccgtagaagt ccatctcgat
     2941 cggcgcgccg ttgtacagcg tcgacgagat gttggagttc cagttgccga gatagaagcc
     3001 cgacgagtgc gaatagtcga tgcccccctg gattgccggc tgatagtcgg tctgggaaaa
     3061 gccccggaac cgatagtcgc tggcgatggt gagattgccg ctgaacgtgt gatccggcgt
     3121 tgctgccgga gctgccgtct gggccgcggc cagcagcgga gcagaacagg cagcggctac
     3181 ggacgctgca atgaatgctt tcttcatgaa ggatttcctt tcgagggatc aaaggtggag
     3241 caaggctcgg gacaggctta cgcaacatgt gtgccaattg ctgcgacgcc aaaaacgcct
     3301 cgaactggtg cgaaaccctg ggcgctcgcc cgttcctgcg tcattcaagt gcgcggacga
     3361 cccgattccg gtcgcgcagc agaacatgcc ccgcgttgag gcctggcgac tgtcccggtg
     3421 aggtgcgctg ctagactggt ttcgacccgt gtcgtggtgg cagccgtagc cctatggaca
     3481 agatttcgtt tctcgatgac ctgcagtcgc gtgtcagctc gctgatccag cagacaccgg
     3541 cggccgacct gcagaagaac ctcaaggcgc tgctgacgca gcagttcgcg cgtctcgaac
     3601 tggtgacgcg cgaggagttc gatacccagt ccaaggtgct ggcgcggacg cgcgccaaac
     3661 tcgacgaact cgaacgccgc ctggccgagc tcgaacagcg aaagacctga gggacggcgc
     3721 cgcggtgcgg cgcgacggtg aatggtcctg tcggacgaca ggacccgcga actgaatgac
     3781 taggccgcgg aagcttcggg cgaagcgcgc ttgcgagcga ccgcgatgat ctttgcggcg
     3841 gtttcgagtt gcaggtcgga cagttcggcg tccatcgtcg gccgatcgat caggcgaacg
     3901 ttgcggcgcc gggcaatttc cttggcgccg cgctcgtagc cggcctccgc tatcacggcc
     3961 acgcgcagca tgccgatctc gcgcgcctgc tcgatcaatg cagtgatgcg atccaccgag
     4021 acgtggtcgt tctgcacgac gaatgcatag gcgcgcgacg gatcatcgcg gtgtcgcagc
     4081 acgagttcga tcggccacac cgcgggagac gccggtacct ggcggtagcc gcgggattcg
     4141 taccaggcga gcaccaattc gcgcagcgga accgacggcg ccatcgaatc cagcatcggc
     4201 gtctgctcgc gggccgccgc gcgggatgcc gcgttggcac gcttgggttc gggcggtgtc
     4261 ggggcactca cgcgcgcggg cgcggcgggg cggccggagt cgtcgaccat gatctgcgag
     4321 atcaattcct cgatcggatc gaggccggcc gcggcctctt cggccggcgt catgttgcgc
     4381 gacgggccgg ggcgcttatg gtcgtactcg atcggcggaa gatccttgct ggcgtggtcc
     4441 tgttcagcgg cctcgccatg ggcctgacgg atcagtgcgg catggtcgat ctcctcgacc
     4501 ggcgccgcag gattcgcctt gctcgctttc gctttcgctt ccggcgccga gctgccgagc
     4561 atcatcgcca tggcggcctg ttcgcgctgt gccgcctgca gacggatcgc ttccgcgcgc
     4621 cgacgcgccc acaggtagag ccctaccagg acgacgagca aaccaattcc gaacagcagt
     4681 gaagacacga aagtgcgggc cggcaccggc gcgtggacgc accggaagga tgtcagggct
     4741 gcaatcgtga cacgagtcgc cccgcaagcc caaccgaata tggggccaaa ggcaccattt
     4801 ttcgtggtgg tggtcgtgct ccgccaggcc ttgcccaacg ctgcaaggta tggatagcgg
     4861 caaagcgcgg tcctattcat cgaattcaat cgacggcggc atggcacgcg ccgatagaat
     4921 cgcccgcgct ggacccccac cccatgtcga tcgccatcgt tcgtacccgc ggcctgtccg
     4981 gcatgtatgc gccgcaggtg acggtcgagg tccatctggc gaacggcctg ccgtcattca
     5041 ccctggtggg tctgcccgac accgaagtac gcgaagcgcg cgagcgcgtg cgtgcggctc
     5101 tcctgaccag ccgcttcgac tttccgaccc ggcggatcac cgtcaatctc gcaccggccg
     5161 acctgccgaa ggagtccggg cgcttcgatc tgccgatcgc gctcggtatc ctggccgcga
     5221 ccggccagat tccgacgcgc acactcgacc aatacgagtt tgccggcgag ctgtcgctga
     5281 ccggggaact gcgaccggtg cgtggcacgc tggcgatggc ctacgcggtc gcaaagtccg
     5341 agcacacggt cggcccccgg cgcggatttg tcgtcccgtt cgaaagcgcc gacgaagccg
     5401 cgctggtcgg cggtatcccg atcttcccgg cacggactct gctgcaggtg gtcgagcacc
     5461 tgcgcgccga cgccggcgat acctcggccc gtgcgatcgc ggagcaccag gcgatggtgc
     5521 gcctgactcc gccgatttac ccggatctct tcgacgtcaa gggccagctc ggtgccaagc
     5581 gcgcgctcga gattgcggcc actggcggcc acagcgtcct gctggtgggg ccgcccggga
     5641 ccggcaagac catgctggcg caacgcttcg ccggcatcct gccgccgatg acaaccgacg
     5701 aagcgctgga gtccgcagcg gtgctcagtc tggtcggtca gttcgcacct cagcgctggg
     5761 cgacgcgcat ctttcgttcg ccgcaccaca cggcgagcgc ggttgcgctc accggcggcg
     5821 gctcgccgcc gatgcccggc gaaatctcac tcgcgcacca tggcgtgctg tttctcgacg
     5881 aactgccgga gttcgaacga cgcgtactgg agacactgcg ccaaccgttc gagtccggga
     5941 cgatcaccgt gtcacgtgcg gcgcgccagg tcgactttcc ttcgcgcttc cagctgatcg
     6001 gcgcgatgaa tccgtgtccc tgcggctact acggacacgc cagcattgcc tgccgctgca
     6061 cgcccgacat ggtggcgcgc taccaggacc ggatctcggg tcccttgctc gaccgcatcg
     6121 acatgcgcgt ggaagtgagt tccgtgccgg aacaggtcat gacctcgctg cccgatggcg
     6181 aaccgacacg ctttgtcgcc gagcgcgtgc gcaagggcta cgaccgcgcg atcgcacgcc
     6241 agggcaaggt caacgatcac ctgcagggcg ccgagctgga ggaagtgtgt tcgttcgacg
     6301 aggcggtgca gaagctgctg cacgccgcca gcgtgcgcct cggctggtcg gcgcgtgcct
     6361 accatcgcgt gctgaaagtc gcccgcacga tcgccgacct cgccgatgcc gacggactgg
     6421 cgccgcacca cgtcgccgaa gcgatccagt accggcgctc gctgcgcgac acgagctaga
     6481 ccggcagtcc gtctcggtct ctacggccgg ccaaggaaca ggtacacggc gcgattctcc
     6541 ttgctggagt agccgaatcc cacatacagc gggccgagcg gggagtcgac gccgaggaac
     6601 acggacccgg ccaggacatt gacgtaaccg tccgcaggct cgccgttcca gaccggtatc
     6661 agcagcttgg tccgcgcggc ctcggccgac gcgccgaggt acaccccttc gaagaacggt
     6721 atgtctccca tcttggtccg gtagacgagc cgcccgaagg tgaagtcctg ggtcttgatc
     6781 tgctggcgct gaaggcccga cagatagagg aagccgccga ggttgaactg gtcgtagatc
     6841 gcgatgtcat tgctgccgac cttgccgccg gccgtcacca ggccctcgac cgtgtgccga
     6901 ccccaggttg cagcgccgtt gagccgcgct tcccatttcg tgtactcgtc gtcggcgccc
     6961 agcgccgaga gtgacgaata gaagcccgcg gacgcgaaat agccccggtg cggaaaattg
     7021 acgctgtcga gccgatcgaa tcgagccacc ccttcgatgg caccgatgga cgtggccagc
     7081 cggctgggaa agagaaagcc gccgctttgc agctcgaacg ttcgccggcc ataggctgca
     7141 ccgaggcgca cctcgccgta ctcgatgatg ttggcaccga catcgaactg cgcgacccac
     7201 gaagtgtcct ggtactcggc gatgcgcaca tcgtccgaga acaggtacca gggatcgatc
     7261 gcgtagcgca gctgcggcgc gatgaagaaa tactggcgcg gcccgagcgg ctgatagaac
     7321 tcggtgccga gcagcacgtt cgtgccgagc acgacatcgt tgcgccattc cgcgccccac
     7381 gcgttgagcc acttggcgcg atgcgacgcg tacaggttga agttggcgtc cttgccgaga
     7441 tcggcatcga gctccagtcc gaagcggaag tagtggcgtt cgggcttttc cttgacgagc
     7501 acaaccaggg tctgcttgcc atcgatctct tcgaccgtgt agttcacgtt ttcgaagtcc
     7561 ccccggccgt agacgcggcg catgtccagg tcgatcctgt cgcgttcgat cggatcaccc
     7621 acctgcgtgc gcagcgactg cagcacgacc accggtgaca cccgcgcggt tccttcgacc
     7681 ttgatcgcct cgacaatgac cggcgcggac gcggtcggcg ccgcctgttt gctgcgcaga
     7741 gcggcgtact gctcgggcgg cagcgacagc gcccgcaggc gatccgccac tttccaggcg
     7801 gcctcctcgc cgatcggtac cgtcgtcatc aggttgtcga agtcggccga agagaaattt
     7861 cccagttccg gcacgatcag cacgtcgccg gcgcgcaggt ccttcaacga gcgcccgacg
     7921 ttctgctcgg tcaggatgtt gatcatctgc agggtcacgc tcagcaccga ggtgatttcg
     7981 tccggccgca gcagcggcgt gccgagattg accgcgatga tgacgtcggc ccccatcgag
     8041 cgggcgatgt cgatcggcag gttgttcgtc aatccgccgt cgaccagctg ccgcttgccg
     8101 atcgtcacgg gcgccacggc gccgggcacc gacatcgagg cacgaattgc cgccaccacg
     8161 ctgccttcct tcaacacaac catctcgccg gtaccgatgt cggtggcaat cgcacgaaac
     8221 gggatcggta gttcgtcgaa gctgcgcacg ttggcgatct gcaccagctt gcgcagttcg
     8281 ccctcgagcg ccaccccggt gacgatgccc ttgggcaggg caactccgtc cttgccgatg
     8341 cccagctccg gtatcaggta gggaagctgg tcgtcggtct tctgccgcat cgactggtcc
     8401 gcacgcggcg gcttgtcggt aaacaacgtc gccgtcgtga tcttcgcgac cgccgcttcc
     8461 atttccggca cggacaaccc ggtggcatag gccgcgccga cgatcgagcc catgctggta
     8521 ccagcgatca cgtcgaccgg aacccgcaac tcctcaagca cctttagaac gccgatatgg
     8581 gcggcgccgc gcgcgccgcc gccggacagc accagtccga ccttcgggcg ttgcttcgca
     8641 gcgggcgtct cggccggtga cgtcggggac gtctgcgcac tggcgccgga caggatcagc
     8701 agcacgctcg cgatcgcaat ggcacgggcg cgcagtcgca gcgtcatggc agtctttcgg
     8761 gatggttggc cggcaccgcg gccggcgggc ggcagtctaa gcgacgtcaa attcgcgccg
     8821 cgccggcgtg cgcatgagtg cgccgaggca actgtggtgc cccgttaata ccgcccgtgt
     8881 gaaccgggtc agcggttcac tggcatcgtg aattccggac ctttgctcgc ggacgcatcg
     8941 ggccagcgct gcatcacgct cttgtagcgg gtatagaagc gaacaccttc ctcaccgtac
     9001 gcgtgatggt cgccgaacag cgaccgcttc cagccaccga aggagtgaaa cgccatcggc
     9061 accgggattg gcacgttgat gccgaccatg cctgcatcca ctcggcgcgc gaactcgcgc
     9121 gcggtaccgc cgtcggtcgt gtaacacgcc acgccgttgc cgagatcatg gccgttgacc
     9181 agctgtagtg cagcggcgaa gtcgggcacg tgcaccatcg tcagcaccgg cccgaagatt
     9241 tcctcg
//
LOCUS       DATIRT010000179         2830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_418942, whole genome shotgun sequence.
ACCESSION   DATIRT010000179 DATIRT010000000
VERSION     DATIRT010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418942"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /locus_tag="VLN25_04295"
     CDS             complement(<1..1066)
                     /locus_tag="VLN25_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HSQ09824.1"
                     /translation="MSLERLFGLMAEKQASDLFLSVGSPITIKINGVCVPINQERLAP
                     QSIVSLLAERLSDSHFRELEDSRELNIGLPVQGIGSFRLSAFLQRGSISAVVRFIPHD
                     IPKLDALHLPEVLKDLVMERRGLILVVGAAGSGKSTTIASMLDHRNELTTGHVLTFED
                     PIEFLFRNKKSIINQREIGSDARTLQAALRSAMRQAPDVIFIGEIRDRDTMSAALAYA
                     MSGHLVVATLHATNSSHALNRVISFYPPETRQALFQDMSVAVKAIVSQRLVRSKRGGR
                     VPSVEVLMNTRHIADLIERGDVQGIKEAMEQSLAPESISFEQSLYSLIQGEQITREDA
                     LAAADSQNNLLWFINNAGKSR"
     gene            complement(1265..2074)
                     /gene="dapD"
                     /locus_tag="VLN25_04300"
     CDS             complement(1265..2074)
                     /gene="dapD"
                     /locus_tag="VLN25_04300"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HSQ09825.1"
                     /translation="MSLEQTINDAWEQRAELSPAAAPAAVRDAVDHAISDLNAGRVRV
                     AEKVGGEWITHQWLKKAVLLSFRLNDNAPMHAGDLSFYDKVQPKFSHLDAEHMRATGV
                     RVVPPAVARRGSYIAKNVVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSGG
                     VGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEENAVLGMGVFIGQSTKIFNRET
                     GEILYGRVPAGSVVVAGSLPSRECNLYCAVIVKQVDARTRSKTSINELLRT"
     gene            complement(2093..>2830)
                     /locus_tag="VLN25_04305"
     CDS             complement(2093..>2830)
                     /locus_tag="VLN25_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019718138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSQ09826.1"
                     /translation="GFRMELERLTPVDWNGVQLVYVCTPGNPTGRVMTLDEWARLFEL
                     ADRHDLIIASDKCYSEIYFDEATPPLGALSAAAQLGRDRYERVVMFSSLSKRSNLPGM
                     RSGFVAGDAEALHKFHLYRTYHGTAMSPPVQAASIAAWSDEAHVVENRRLYAQKFEQA
                     TPVIARQLPCTRPDAAFYLWAQTPVADTEVARRLYAQKNVTVLPGSYLSREANGANPG
                     TNFVRIALVAEPAEVAEAAARIASLQL"
BASE COUNT          501 a          885 c          943 g          501 t
ORIGIN      
        1 agcgggactt gccggcattg ttgatgaacc agagcaggtt gttctgcgaa tcggccgcgg
       61 cgagcgcgtc ctcgcgcgtg atctgctcgc cctggatcag cgagtacagg ctttgctcga
      121 acgagatgct ctcgggcgcc aaactctgtt ccatcgcttc cttgatcccc tgcacgtcgc
      181 cacgctcgat caggtcggcg atgtggcgcg tattcatcag cacctcgacc gaggggacgc
      241 gtccaccgcg cttggaacgt accagccgct gcgagacgat cgccttcacc gccaccgaca
      301 tgtcctggaa cagcgcctgg cgggtttccg gcgggtagaa gctgatcacg cggttcagcg
      361 cgtgcgagct attggtcgca tgcagcgttg ccacgaccag gtggccggac atcgcatagg
      421 cgagcgccgc gctcatcgtg tcgcgatcgc ggatctcgcc gatgaagatg acgtccgggg
      481 cctgccgcat cgcgctgcgc agggccgcct gcagcgtgcg ggcatcggaa cctatctcgc
      541 gctgattgat gatcgacttc ttgttgcgga acaggaactc gatcgggtcc tcgaaggtca
      601 gcacatgccc ggtcgtcagt tcgttgcggt ggtcgagcat cgaagcgatg gtcgtcgact
      661 tgccggaacc ggccgcgccc acgacaagga tcaggccgcg ccgctccatg acgaggtctt
      721 taaggacctc gggcaggtgc agtgcatcga gcttgggaat gtcgtgcggg atgaagcgga
      781 ccacggcgga gatcgagccc cgctgcagga acgccgagag gcggaagctg ccgatgccct
      841 gcaccggcag gccgatgttc agctcgcgcg agtcctcgag ttcgcggaag tgcgaatcgg
      901 acaggcgctc ggccagcagc gaaacgatcg actgcggcgc gagccgctcc tggttgatcg
      961 gcacgcagac gccgttgatc ttgatcgtga tcggcgagcc aaccgagagg aacaggtccg
     1021 aggcctgctt ttcggccatc aggccgaaca ggcgctcgag cgacatgggc gtagccgaca
     1081 tggaatgcga cctcgtgctg cagagggtca cagcatacga ccggcgggca tcccttgcga
     1141 atccgtccga tcggatgaaa agagggcgga agaggcgtca gggaggtgcc gggcggggtg
     1201 cgcggcgctg gtcgcgcgac cgcgaaggcg cgggacgcgc aacggcatcc agtgtccggt
     1261 acgcctacgt cctcagcagt tcgttgatgc tggtctttga gcgggtccgg gcatcgacct
     1321 gcttcacgat gaccgcgcaa tagagattgc actcgcgcga gggcagtgaa ccggcgacga
     1381 cgaccgaacc ggcgggtacg cggccgtaca ggatctcacc cgtctcgcga ttgaagatct
     1441 tggtgctctg cccgatgaag acacccatgc cgagcacggc gttctcctcg acgatgacgc
     1501 cctcgacgac ctccgagcgc gcgccgataa agcagttgtc ctcaatgatg gtcggattcg
     1561 cttgcagcgg ctcgagcacg ccaccgatgc cgactccgcc ggacagatgg acgttcttgc
     1621 cgatttgcgc gcacgaaccg accgtggccc aggtgtctac catcgttccc tcgtccacgt
     1681 aagcgccgat gttgacgtac gacggcatca gcacgacgtt ttttgcgata tagcttccgc
     1741 gccgcgccac ggcgggcggc accaccctta cgccggtagc gcgcatgtgc tctgcatcga
     1801 gatgcgagaa cttgggctgc accttgtcgt agaaacttag gtcgccggcg tgcatcggcg
     1861 cgttgtcgtt gaggcggaac gacagcagga cggccttttt cagccattgg tgcgtgatcc
     1921 actcgccccc gaccttctcg gcaacccgca cgcggccggc gttgaggtct gaaatagcgt
     1981 gatccaccgc atcgcgcacg gccgcgggcg cggcagcggg cgagagctcg gcgcgctgtt
     2041 cccatgcgtc gttgatggtc tgttcgagcg acatcggtcg tcctcattgg ggtcagagtt
     2101 gtagggaggc gatgcgcgcc gcggcctcgg ccacttcggc cggttcggcg acgagcgcga
     2161 tgcgaacgaa gttcgtgccg gggttcgctc cgttggcctc acgcgacagg tagctgcccg
     2221 gcagcaccgt tacattcttc tgggcatata gccgccgcgc aacctcggta tcggcaaccg
     2281 gtgtctgcgc ccaaaggtag aacgcggcat ccggccgcgt gcaggggagc tgacgggcga
     2341 tgaccggtgt ggcctgctcg aacttctgcg catagagccg gcggttctct accacatggg
     2401 cctcatcgga ccaggccgcg atgctcgccg cctgcaccgg agggctcatc gcggtgccgt
     2461 gataggtgcg ataaaggtgg aacttgtgca aagcctcggc atcgccagcg acaaaaccgg
     2521 acctcattcc gggcaaattc gagcgcttcg acaagctgga gaacatgacc acgcgctcgt
     2581 agcgatcgcg tccgagctgc gccgcggccg agagcgcccc gagcggcggg gtggcttcgt
     2641 cgaagtagat ctcggagtag cacttgtccg aggcgatgat taagtcgtgc cggtcggcca
     2701 gttcgaacag ccgggcccat tcgtccagcg tcatcacgcg accggtggga ttgccgggcg
     2761 tacagacgta gaccagctgg acgccgttcc aatcgactgg tgtcaaccgc tcgagctcca
     2821 tgcgaaagcc
//
LOCUS       DATIRT010000180         2964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1402355, whole genome shotgun sequence.
ACCESSION   DATIRT010000180 DATIRT010000000
VERSION     DATIRT010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402355"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..261)
                     /locus_tag="VLN25_04310"
     tRNA            complement(185..261)
                     /locus_tag="VLN25_04310"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(225..227),aa:Pro,seq:ggg)
     gene            complement(312..692)
                     /locus_tag="VLN25_04315"
     CDS             complement(312..692)
                     /locus_tag="VLN25_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HSQ09827.1"
                     /translation="MQRHETSQLLPPIPAKRYFTIGEVSELCGVKPHVLRYWEQEFTQ
                     LRPVKRRGNRRYYQHHEVLLIRRIRELLYEQGFTISGARNRLEEGERPSRHEGKNGNG
                     AQIPLDAVDLREELAAIRRLLAGN"
     gene            complement(739..1125)
                     /locus_tag="VLN25_04320"
     CDS             complement(739..1125)
                     /locus_tag="VLN25_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HSQ09828.1"
                     /translation="MRDDSQMTTGFKFPELPPATGGNHASDRGAKTMTKAELAEALFD
                     RLGLNKREAKDMVDGFFDEIRQALERGESVKLSGFGNFQLRDKPTRPGRNPKTGEEIA
                     ITARRVVTFHASQKLKAAVARIAGSH"
     gene            complement(1131..>2964)
                     /gene="pheT"
                     /locus_tag="VLN25_04325"
     CDS             complement(1131..>2964)
                     /gene="pheT"
                     /locus_tag="VLN25_04325"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HSQ09829.1"
                     /translation="LPVSVEAADLCGRFSGRVIRGVDAHEGTPEWMKQRLLRSGQRPI
                     SALVDISNYVMLELGRPSHVFDLDKVKGGVCVRWGRPGETVELLNGQTVKVDGGVGVI
                     ADDTGVEALAGIMGGEATAVSLDTRNIYVEAAFWWPDAIRGRARRFNFSTDAAHRFER
                     GVDYATTTDHVEYITRLILDVCGGQPGPMDDTIVGLPRRAPVSMRVERCRKVLGIALG
                     ADDMANVFERLRLPYHRSEAAFIVTPPSYRFDLETEEDLIEEVARLHGYARIPAHAPL
                     APAHMRALPETRRSPHAVRALLAQLGYQELINYSFVDARWEHDFAANDQPIAVLNPIS
                     AQMSVMRSSLLGGLVSVLGYNLNRKVTRVRVFEIGRVFWRDPQVVDGPLEVQGIAQPT
                     CVAGLAYGTADDDQWASASRDVDYFDVKADVERLFAPGVIRVEPAVHPALHPGRSARI
                     RYDGREVGFLGELHPRLQQAYELPKSAVVFQLELEPLLARELPVVRDIPKFQPLQRDI
                     AVVVSEGVGYASIEGAIDNLSRADGRLSALTEVRLFDVYRPRSTSESAAVVGANALLN
                     KEKSLAIRIVLQDTERPLSDTDADAAVTAVVEELARRFGARLRQ"
BASE COUNT          535 a          956 c          919 g          554 t
ORIGIN      
        1 gtcgtcgacg ctcgttccgc ggtcgaactc agcttccaat ccaggaccgc cgaggagagc
       61 cacatgggtc tgccgtgtga tgcgctcatc cgcaccgtcc attgtttccg aacgcagttc
      121 cgaacgtgag agccggaaag agcagcgggc cagcacgttg tgagtgctaa cccgttgatt
      181 tcattggtcg ggacggcggg attcgaactc gcgacccctt gcaccccatg caagtgcgct
      241 accaggctgc gctacgcccc gaaggagcgg attatagccg cgactgcgac ctgtcccggg
      301 aaaaaacgcg atcagttgcc ggcaagcagc ctgcggatgg ccgcaagttc ctcgcgcaaa
      361 tcgaccgcgt cgagtggaat ctgcgcgcca ttgccattct tgccttcgtg ccgcgaaggg
      421 cgttcacctt cttcaaggcg gttgcgggcg ccactgatcg tgaagccctg ctcgtacagt
      481 agttcgcgga tgcgacggat cagcaggact tcatggtgct ggtagtagcg ccggttgccg
      541 cgccgcttga ctggccgcaa ctgcgtgaac tcctgctccc agtaacgcag cacatggggc
      601 ttgaccccgc acaactcgct cacttcacca atcgtgaagt agcgcttcgc cggaatcggc
      661 ggcaacagtt gcgatgtctc gtggcgttgc atcgcgctgg tcgggaagtc cgctgctcag
      721 cccggcacgc tcgcggccct aatgggaacc cgcgatacgt gccaccgccg ctttcagctt
      781 ctggctggca tggaaagtca caacgcgacg cgccgtgatg gcgatttctt cgccggtctt
      841 cggattgcgg cccggtcgcg tcggtttgtc acgcaattga aagttcccga agccggaaag
      901 cttcacggat tcccctcgct cgagcgcctg ccggatctcg tcgaagaacc cgtccaccat
      961 gtccttggct tcgcgtttgt tcagtcccag ccggtcgaag agggcttcgg ccagttcggc
     1021 cttggtcatt gtcttggccc cgcgatcgct cgcgtggtta ccacccgtgg caggcggcaa
     1081 ctccggaaac ttgaagcccg tcgtcatctg ggaatcgtct cgcattattt tcactgtcgt
     1141 agacgggcgc cgaaccgccg ggccagttcc tcgacgactg cggtgaccgc tgcatcggca
     1201 tcagtatcgc tgagcggcct ttcagtatct tgcagaacaa tccggatagc aaggcttttt
     1261 tctttattta acaatgcgtt agcgcccact actgcggctg attccgaagt tgaccgggga
     1321 cgatacacgt cgaacagtcg gacttccgtc aaggccgaca atcggccatc ggcgcgcgac
     1381 aggttgtcga ttgcgccttc gatcgaggcg tacccgacac cctcgctgac gacaaccgcg
     1441 atgtcgcgct gtagcggttg gaacttcgga atgtcgcgca cgacgggaag ctcgcgcgcg
     1501 agcaacggtt caagttcgag ctggaagact acggcgctct tcggcaattc gtaggcctgc
     1561 tgaagacgcg ggtgcagctc cccgagaaaa ccaacctcgc gtccgtcgta gcggatgcgc
     1621 gcgctgcgtc ccggatgcaa cgcaggatgg acggccggct cgacgcggat gacgcccgga
     1681 gcgaacagtc gctccacgtc tgccttgacg tcgaagtagt cgacgtcgcg cgacgcgctc
     1741 gcccactgat cgtcgtcagc cgttccatac gcgagaccgg cgacacaggt aggctgggca
     1801 attccctgca cttccagcgg accgtcgacg acttgcgggt ctcgccagaa cacgcgcccg
     1861 atctcgaaga cccggacgcg tgtcaccttg cggttcaggt tgtagcccag cacggacacc
     1921 agaccgccca gcagtgaact gcgcatcacc gacatctgcg ccgagatcgg gttgagcacg
     1981 gcgatgggct ggtcgttcgc ggcgaagtcg tgttcccagc gggcgtcgac gaacgaatag
     2041 ttgatgagtt cctggtagcc gagctgggca agcaatgcgc gaaccgcatg cggcgaccgc
     2101 cgcgtttcag ggagtgcgcg catgtgcgcc ggcgccaatg gcgcgtgcgc aggaatgcgc
     2161 gcgtagccgt gcaaccgcgc cacctcctcg atcaggtcct cctcggtttc gagatcgaac
     2221 cggtagctcg gcggcgtcac gatgaacgcg gcctccgagc ggtgataggg caggcgcagg
     2281 cgctcgaata cgttggccat gtcgtcggcg ccgagcgcaa tgccgagcac cttgcggcaa
     2341 cgctccacgc gcatgctgac cggcgcgcgt cgcggcaacc cgacgatcgt gtcatccatc
     2401 gggcccggct gcccgccgca tacatcgagg atcaggcggg tgatgtattc gacgtgatcg
     2461 gtggtggtcg cgtagtcgac gccgcgttca aatcgatgcg ccgcatcggt ggaaaaattg
     2521 aaacgacggg cgcgcccgcg gatggcatcc ggccaccaga atgccgcctc gacgtagatg
     2581 ttgcgcgtgt cgagcgagac ggcggtggct tcaccgccca tgatccccgc cagtgcctcg
     2641 accccagtgt catccgcgat cacgccgaca ccgccgtcga ctttgacggt ctgtccgttc
     2701 agcagttcca cggtttcgcc ggggcggccc cagcggacac agaccccacc cttcaccttg
     2761 tcgaggtcga acacatgcga cgggcgtccg agttccagca tcacgtagtt cgagatgtcg
     2821 accagtgccg agatcggtcg ctggccactg cgcagcagtc gctgcttcat ccactccggt
     2881 gtgccttcat gggcatcgac gccgcggata acgcgtccgg aaaatcgacc gcacaggtcg
     2941 gcggcttcca cagaaacggg cagt
//
LOCUS       DATIRT010000181         3236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_910607, whole genome shotgun sequence.
ACCESSION   DATIRT010000181 DATIRT010000000
VERSION     DATIRT010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910607"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1693
                     /locus_tag="VLN25_04330"
     CDS             <1..1693
                     /locus_tag="VLN25_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSQ09830.1"
                     /translation="DPDVLRCEALFDRTADRFLRDWVGREFPATRFAAYFAQLMNRQP
                     VVVNDTRTSRLTTMFAGDQHWSHVRALLDVPILVEGTIAGVLCLQHRDIRYWDEDDVG
                     FANTTGLMLALSMEAAQRHEAESRIEHLAWYDALTGLPNRNLLRETMRDMVMTASNRK
                     RRMAAMLIDLDRFKDVNDTLGHLVGDALIKSASDVLRETVGEDGTVARLGGDEFVVLI
                     SEFVHRQEVALLAARIAQALHRTDLVSNIDTQVSASIGVALFPEHGRDMSTLLKNADA
                     AMYQAKRDGRNQFSFFNPIRYERAAREVQLGIELVKAVQSESPQFICEYQPQIEMASG
                     RVVGLEALIRWNHPVHGMLTPDRFIGVAEVSGLSERITRWVLNEVCSQINRWRLVRPD
                     FDVPVAVNVAGREMGSTALPILVRSALVKHGIEPRLIVLEITERTLVKESEINNDVLA
                     ELASLGVGLVLDDFGTGYSMLGYLKRMPIHALKIDQSFIEGIPADPDSRAIVHAMLAV
                     ARHFRLKVVAEGVESQDQVDYMRDIGCEFAQGFFYSRAISGDTILDYLARESPAP"
     gene            1746..2522
                     /locus_tag="VLN25_04335"
     CDS             1746..2522
                     /locus_tag="VLN25_04335"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HSQ09831.1"
                     /translation="MAYQNILLETRGRVGLLTLNRPKALNALNDQLMDELGEALLAFD
                     ADEAIGAMVVTGSEKAFAAGADIGAMKEWTYMDVFKSEYISRNWETIRRVRKPVIAAV
                     GGYALGGGCELAMMCDIIIAADTAKFGQPEIKLGIIPGAGGTQRLPRAVGKAKAMDLV
                     LSARMMDAAEAERAGLVSRVVAAEKLLDEAIAVAAAIASHSLPAVMMAKEAINRAYEA
                     PLNEGMLFERRLFHSLFATNDQKEGMAAFVEKRPAQFRHT"
     gene            3104..>3236
                     /locus_tag="VLN25_04340"
     rRNA            3104..>3236
                     /locus_tag="VLN25_04340"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          634 a          986 c         1025 g          591 t
ORIGIN      
        1 cgatcccgac gtgctgcgct gtgaggcact gttcgatcga accgccgacc gcttcctgcg
       61 cgactgggtc ggccgagagt ttcctgcgac ccggttcgcc gcttacttcg cgcaactgat
      121 gaatcgccag ccggtcgtcg tcaatgacac gcgtacctcg cgcctgacaa ctatgtttgc
      181 gggcgatcag cactggtcgc acgtgcgtgc gctgctcgac gttccgatac tcgtcgaggg
      241 caccatcgcc ggtgtccttt gccttcaaca tcgcgacatc cgatactggg acgaggacga
      301 tgtcggcttc gccaacacga ccggtctgat gctggccctg tcgatggagg cggcgcagcg
      361 gcatgaagct gaatcgcgca tcgagcatct tgcctggtac gacgcgctga cggggctgcc
      421 gaaccgcaac ctgctgcgag agaccatgcg cgacatggtc atgacggcgt cgaaccgcaa
      481 gcgtcgcatg gcagcgatgt tgatcgacct cgaccgcttc aaggacgtca acgacaccct
      541 cggacacctg gtcggggacg cgctgatcaa gtcggcgtcc gatgtgctgc gcgaaaccgt
      601 cggcgaagac ggcaccgtcg cccgactggg cggcgacgaa ttcgtcgtgc tgatcagcga
      661 gttcgtgcat cgccaggagg tcgcgctgct ggcggcccgg atcgcacagg cactgcaccg
      721 gaccgatctc gtgtccaaca tcgataccca ggtgtcggca tcgatcggtg tggcgctctt
      781 tcccgagcat ggccgagaca tgagcacgct gctgaagaat gccgacgcgg cgatgtacca
      841 ggccaaacgc gatgggcgca accagttcag cttcttcaat ccgattcgat acgagcgggc
      901 ggcacgcgaa gttcagctcg gcatcgagct ggtcaaggcc gtgcagagcg aaagccccca
      961 gttcatctgc gaataccaac cgcagatcga aatggcaagc ggtcgcgtcg tcgggctcga
     1021 ggcgctgatc cgatggaatc acccggtgca cggcatgctt acacccgacc gtttcatcgg
     1081 ggtggccgag gtcagcggcc tgtcggagcg catcactcgc tgggtgctga acgaagtctg
     1141 cagccagatc aatcgctggc ggctcgtgcg gcccgacttc gatgtcccgg tggcggtgaa
     1201 cgtcgcaggt cgcgagatgg gctcgaccgc cctgccgatc cttgttcgca gcgctctcgt
     1261 caagcacggc atcgagccac gcctgatcgt tctcgaaatc accgagcgga cactggtcaa
     1321 ggaaagcgag atcaacaacg acgtgctggc cgaactcgca tcgctgggcg tcggcctggt
     1381 gctcgacgac ttcggcacgg gctactcgat gctgggttac ctgaagcgca tgccgataca
     1441 tgcactcaag atcgaccagt cgttcatcga gggtattccg gccgaccccg acagccgtgc
     1501 catcgtgcac gccatgctgg cggtagcgcg ccacttccgc ctgaaggtcg tcgccgaagg
     1561 ggtcgagtcg caggaccaag tcgactacat gcgcgacatc ggctgcgagt tcgcacaggg
     1621 cttcttctat tcgcgcgcga tctccggcga cacaatcctc gactacctcg cgcgcgagtc
     1681 accggcgcct tagcctcggt cccggtgacc gctaaactcg ctggacattg cgaggaggca
     1741 tctctatggc gtaccagaac atcctgcttg agacccgtgg tcgcgttggc ctgctaacgc
     1801 tgaatcgtcc aaaggcgctc aatgcactca acgaccagct gatggatgaa ctgggcgagg
     1861 ccctgctcgc gttcgatgcg gacgaagcaa tcggtgcgat ggtcgttacc ggaagcgaaa
     1921 aggcgttcgc ggccggagcc gacatcggcg cgatgaaaga gtggacttac atggacgtct
     1981 tcaagtccga atacatctcc cgcaactggg agacgatccg ccgcgtgcgc aaaccggtga
     2041 tcgccgccgt cgggggttat gcgctgggcg gcggctgcga actggcgatg atgtgcgaca
     2101 tcatcattgc ggccgacacg gcgaagttcg ggcaacccga gatcaaactg gggatcatcc
     2161 cgggtgcagg tggaacgcag cgcctgccgc gcgccgtcgg gaaggccaag gcgatggacc
     2221 tcgtgctctc ggcgcgcatg atggatgcgg ccgaagcgga gcgtgcgggc ctggtgtcac
     2281 gcgtggtggc tgcagaaaag cttctcgatg aagccatcgc agtcgcggca gccatcgcca
     2341 gccattcgtt gccagcggtg atgatggcca aggaagcgat caatcgcgcc tatgaagcgc
     2401 cgctgaacga aggcatgctc ttcgagcggc gcctgtttca ttcgctcttt gcgaccaacg
     2461 atcagaagga aggcatggcg gccttcgtcg agaagcgtcc ggcgcagttt cgtcacacgt
     2521 aggggtggtc agcagcatcg gctgcagcgt tgcgctggct ttggcgcgtc atggacagac
     2581 ccctagagga tcccgtctag cgctcggctc cgggacacgc gggcttgtgc gcgtgcgacg
     2641 gttggtcccg ttacggaaca aggatttatc gcctgttcgc acgtggccaa gccgcgtcgc
     2701 atcattgccc gcggtcgcct gaatcgccca gcgatttggg gcttgggtgc cccgctagcc
     2761 gcgtctcaga gcgttgccgc gaccggtcaa cttgacacta attttgccgg gccccataat
     2821 tccgcttctc tgctgcttcg gttgcgtcgc aacacgacga tctgaccggg cggcacgttc
     2881 tttaacaacg aaacagccga taagtgtggg cgcttggtga aaggcgcaac cggcacggaa
     2941 ggtcgtcgag gtctcacacc tagacatcaa ccgactgcgc cggctgaact caaaacatca
     3001 ggtgctcacg caatggaaag agggaaatga tggaaacatc acatacctcg attccagcga
     3061 gtgagtaatc aatcgtgcct agcacggttg cgcaaacaga atcgaactga agagtttgat
     3121 cctggctcag attgaacgct ggcggcatgc cttacacatg caagtcgaac ggcagcacgg
     3181 gggcaaccct ggtggcgagt ggcgaacggg tgagtaatgc atcggaacgt gcccag
//
LOCUS       DATIRT010000182         2297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1795834, whole genome shotgun sequence.
ACCESSION   DATIRT010000182 DATIRT010000000
VERSION     DATIRT010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1795834"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1983)
                     /gene="glnE"
                     /locus_tag="VLN25_04345"
     CDS             complement(<1..1983)
                     /gene="glnE"
                     /locus_tag="VLN25_04345"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HSQ09832.1"
                     /translation="MTLSLELAAADWRGVPATLADVAELSHYARRALDLEVGHDSPAD
                     ARLALLKDLASAAWTAERMRAALDHDIPAAREAPQLATDLRRLRRRVMLSLIARDVTG
                     AAGLAEVVQTTTALAEVALQSALALHAHELALVHGIPSSDNGSPQDLLIVGMGKLGGV
                     ELNVSSDIDLVFVYDEDGSKRATGAYAAAVRPLTNHEFFERLGRRIIAALSDTTGDGF
                     VFRVDMRLRPNGDAGPLAVSTAMFEEYLVALGREWERFAWLKGRIVSAPVFATPAAFA
                     AQCASLEEIVRPFVFRKYLDFGAIASLRDLHALIRAETGRRSAGRDDRGDNVKLGRGG
                     IREIEFLAQTFQVIRGGREPRLRSRSTLATLACLADLGLLPRDTSTRLAAGYEFLRDL
                     EHALQYVDDAQTHLLPVDPQARARVAALLGARSADTMMAEFRAVQDTVAGTFDAVFVE
                     PVTERSPLPMGAGPADGAADEAELREHLQAMGFADPAATAQRLRGVLASRRVQVTSEA
                     TRSRIERLLKSGLQAVTARARATGASHDIGPDELFARFAQLVDVIAGRSTYIALLNQF
                     PPALERVMRLLAASRWATDYLVRHPILLDELLDERVELFDNEHTPDWMAWASGVRAQL
                     AEADGDQEEQMNLLRDVHHAQVFRLLVADLDGRLTVE"
BASE COUNT          376 a          797 c          773 g          351 t
ORIGIN      
        1 ctcgaccgtc agccgcccat ccagatcagc caccagcaga cggaacacct gcgcgtgatg
       61 tacgtcgcgc agcaggttca tctgctcttc ctggtcgccg tcggcctctg ccagctgcgc
      121 gcgcacgccg ctcgcccagg ccatccagtc gggcgtgtgc tcgttatcga acagctccac
      181 ccgttcgtcg agcagttcgt ccagcaggat ggggtgacgc acgagatagt ctgtcgccca
      241 gcggctggcc gcgagcaggc gcatcacgcg ctcgagcgcc ggtggaaact ggttcagcag
      301 cgcgatgtag gtgctccgac cggcaatcac gtcaaccagc tgagcgaagc gcgcaaagag
      361 ctcgtccggg ccgatgtcat gcgacgcacc ggtcgcgcgg gcacgcgccg tcaccgcctg
      421 caggccggac ttcagcagtc gctcgatgcg cgagcgcgtg gcctcgctgg tgacctggac
      481 ccggcgcgaa gcaagcacgc cgcgcagccg ctgcgccgtg gcggcgggat cggcaaaccc
      541 catcgcctgc aggtgctccc gcagttccgc ctcatcggct gcgccgtcgg caggcccggc
      601 tcccatgggc agtggcgagc gctccgtcac cggttcgacg aagactgcgt cgaacgtacc
      661 cgccacggtg tcctgcaccg cgcgaaactc ggccatcatc gtgtcggcac tgcgggcgcc
      721 gagcaatgcc gcgacacggg cccgcgcttg tgggtcgacc ggcagcaggt gcgtctgggc
      781 atcgtcgacg tactgcagcg catgctcgag gtcacgcagg aattcgtatc cggccgcgag
      841 ccgcgtgctg gtgtcacgag gcagcaagcc gaggtcggcc aggcaggcaa gggtcgcgag
      901 cgtcgagcgc gagcgcaagc gcggctcgcg accgccgcgg atcacttgaa aggtctgcgc
      961 aaggaactcg atctcgcgta tgccgccgcg gcccagcttc acgttgtcgc cacgatcgtc
     1021 gcggcccgcg ctgcgccgcc cggtttcggc gcggatcagg gcatgcaggt cgcgcagcga
     1081 ggcaatcgcg ccgaagtcca ggtacttgcg aaacacgaag ggccgtacga tctcctcgag
     1141 tgacgcgcac tgggcggcga aagccgccgg cgtggcaaag accggcgcgg aaacgatgcg
     1201 gcccttgagc caggcgaagc gctcccactc acggcccagc gccaccaggt attcctcgaa
     1261 catcgcggtc gacacggcca gcggcccggc atctccattg ggtcgcaggc gcatgtcgac
     1321 gcggaacacg aagccgtcgc ccgtggtgtc cgacagtgca gcgatgatgc gccgtccgag
     1381 gcgctcgaag aattcgtggt tcgtcagcgg ccgcaccgcg gccgcatagg cgccggtcgc
     1441 acgcttcgac ccgtcctcgt catagacgaa gaccaggtcg atgtctgaag acacattgag
     1501 ttcgactccg cccagcttgc ccatgcccac gatcagcagg tcctgcggcg acccgttgtc
     1561 actgctggga atgccgtgca ccagcgccag ttcgtgcgcg tgcagtgcga gcgcggattg
     1621 cagcgcgacc tcagccagcg cggtggtggt ctgcacgacc tctgccagcc ccgcggcccc
     1681 cgtgacgtcg cgcgcgatca gcgacagcat cacgcggcgg cgcaatcgcc gcaagtcggt
     1741 ggcgagctgc ggcgcctcgc gcgcggctgg tatgtcgtgg tcgagcgccg cccgcatgcg
     1801 ttccgccgtc caggcggccg acgcaaggtc cttcaacagt gcgagccgcg catcggccgg
     1861 actgtcgtgc ccgacctcga gatcgagcgc gcgccgcgcg tagtgcgaca gttccgcgac
     1921 gtcagccagc gtggcgggaa cgccgcgcca gtccgccgct gcaagttcga ggcttagggt
     1981 cacgacagga gcaggttgac ggggcaggtc atggcgggca gcgggcgagc gaaggaggac
     2041 agctccggag aggcgcgcac actcggcgca ctgcacctct gtgatagagt gacttcgcct
     2101 tgtcagggcg gccgccgccg taggcgtttg cctcctgcgt attgcggtcg acaccactgc
     2161 gggcgacatc gaaccattgc gggcgacccc caaccattcc gggcaacagc gaaccgattg
     2221 tagtttcttc gacgacgagc cgcttcgtcc gcacaccgcg gtcattgaat ctcgcttcat
     2281 tttgctttgc cttgtcg
//
LOCUS       DATIRT010000183         2713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1795846, whole genome shotgun sequence.
ACCESSION   DATIRT010000183 DATIRT010000000
VERSION     DATIRT010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1795846"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..2679)
                     /gene="gyrB"
                     /locus_tag="VLN25_04350"
     CDS             complement(139..2679)
                     /gene="gyrB"
                     /locus_tag="VLN25_04350"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HSQ09833.1"
                     /translation="MSDIPNSPDNLPKPIDPYGESSIQILEGLEAVRKRPGMYIGDTQ
                     DGTGLHHMVFEVVDNAIDDALAGHCDDIVVTIHTDNSISVVDNGRGIPTGIKFDDKHE
                     PRRSAAEIALTELHAGGKFNQNSYKVSGGLHGVGVSCVNALSKWLRLTIRRDGKVHEL
                     EFRQGEVQDRVIETLNSQEGPVEASPMKVAGPTDKRGTEVHFLPDDAIFGVVDFHYEV
                     LSKRLRELSFLNNGVRIRLVDQRTGKEEDFAFAGGVKGFVQYINKTKTVLHPNAFYAT
                     GEAGGAASSSTAAVPIGVEVAMQWNDGYSESLLAFTNNIPQRDGGTHITGLRAAMTRV
                     LNNYIKANELDKKAKVETTGDDMREGLTCVLSVKVPEPKFSSQTKDKLVSSEVRPAVE
                     DVVGKALESFLLERPADAKIICGKIVEAARAREAARKAREMTRRKGVLDGVGLPGKLA
                     DCQEKDPAQAELYIVEGDSAGGSAKQGRDRKFQAVLPLRGKVLNVEKARFDKLISSEQ
                     IATLITALGTGIGTGPENEGFNLEKLRYHRIIIMTDADVDGAHIRTLLLTLFYRQMPL
                     LVERGHIYIAQPPLYKVKHGKDERYIKDDTELAQYMVTLALKDAQLVPAEGRAPIEGT
                     ALEELARQYLVANAVVERLARVIDRATLEMLMAGVELNLDDAVAAESSGRRLAAALGN
                     STVHVYPQHDDKTDKHRLRIERQHHGNIRATVVDADFVRGADYAALAGAAQTFKGLVG
                     SDARVRRGSGEKQKETTVSDFREAMDWLMKEAESAVSRQRYKGLGEMNPEQLWETTMD
                     PAVRRMLKVQIEDAIGADQIFTTLMGDEVEPRRAFIESNALYAGNIDV"
BASE COUNT          438 a          872 c          832 g          571 t
ORIGIN      
        1 tgggaaatct gctgaaagag tggccccatg ccttcccctg acgccggagc ccagcgggct
       61 ccgagctggc tgataaaaga agggccctaa ctccgatgga gtgaaggcct cacttagccg
      121 atgtgcacct acagatatct aaacgtcgat attccccgca tacagtgcat ttgattcaat
      181 gaaagcgcgc ctgggctcaa cctcgtcgcc catcagtgtg gtgaatatct gatcggcgcc
      241 gatggcgtct tcgatctgca ccttgagcat gcgtcgcacg gccgggtcca tggtggtttc
      301 ccacagttgc tcggggttca tctcgcccag gcccttgtag cgctggcggc tgacggcgga
      361 ctcggcttcc ttcatcagcc agtccatcgc ctcgcggaag tcggaaaccg tcgtttcctt
      421 ctgtttctcg ccgctgcctc gcctgaccct ggcgtcacta ccgacaaggc ccttgaaggt
      481 ctgagccgcc cccgcgagcg cggcatagtc ggcgccccgc acgaagtccg catcgaccac
      541 cgtggcgcgg atgttgccgt ggtgctggcg ttcgatgcgc agacgatgtt tgtccgtctt
      601 gtcgtcatgc tgcgggtaga catgcacggt cgaattgcca agcgcagcgg ccaggcgccg
      661 gcccgaggac tcggcggcga ctgcatcatc gaggttgagt tcgacgccgg ccatcagcat
      721 ttcgagcgtg gcgcggtcga tcacgcgcgc gagacgttcg accaccgcgt tggcgacgag
      781 gtactggcgc gccagctcct cgagtgcggt gccttcgatc ggggccctgc cctcggccgg
      841 caccagctgc gcatccttca gcgcaagcgt gaccatgtac tgcgcgagct cggtatcgtc
      901 cttgatgtag cgctcgtcct tgccgtgctt gaccttgtac agcggcggct gcgcgatgta
      961 gatgtgcccg cgttcgacca gcaacggcat ctggcggtaa aagagcgtca gcagcaacgt
     1021 acgaatgtgc gctccgtcga cgtcggcgtc ggtcatgatg atgatgcggt ggtagcggag
     1081 cttctcgagg ttgaaaccct cattctccgg cccggtgccg atgccggtgc cgagtgcggt
     1141 gatcaaggta gcgatctgtt ctgagctgat cagcttgtca aagcgcgcct tctccacgtt
     1201 caacaccttg ccccgcagcg gcagcacggc ctggaatttg cgatcgcgcc cctgcttggc
     1261 cgagcctccc gccgagtcgc cctcgacgat gtaaagctcg gcctgtgccg gatccttctc
     1321 ctgacagtcg gcgagctttc ctggcagtcc cacgccgtcg agcacgcctt tgcgacgcgt
     1381 catctcgcgt gccttgcgcg cggcttcgcg cgcacgagca gcttcgacga tcttgccgca
     1441 gatgatcttg gcatcggccg gccgctcaag caggaaactt tcgagcgcct tgcccacgac
     1501 gtcctcgacc gctggccgca cttcggacga caccaacttg tccttcgtct gcgaactgaa
     1561 cttgggctcg gggaccttga ccgacaggac acaggtgagt ccttcgcgca tatcgtcgcc
     1621 ggtggtctcg accttggcct tcttgtcgag ctcgttggcc ttgatgtagt tgttcagtac
     1681 ccgcgtcatc gcggcgcgca ggccggtgat gtgcgtgccg ccgtccctct gcgggatgtt
     1741 gttggtgaag gcgagcaggc tctcgctgta gccgtcgttc cactgcatcg cgacttcaac
     1801 gccaatcggc accgccgccg tgctgctgct ggctgcgcct cccgcttccc cggtcgcgta
     1861 gaaagcgttc gggtgaagca cggtctttgt cttgttgatg tactggacga aacctttcac
     1921 gccgccggcg aacgcaaagt cctcctcctt gccggtgcgc tgatccacca gccggatgcg
     1981 cacgccattg ttgaggaacg agagctcgcg cagccgcttg ctgaggacct cgtagtggaa
     2041 atcgaccaca ccgaagatcg cgtcatcggg cagaaagtgc acttcagtac cgcgcttgtc
     2101 ggtcgggccg gcgaccttca tcggcgacgc ctcgaccggc ccttcctggg aattcagggt
     2161 ctcgatgaca cggtcctgca cctcaccctg gcggaactcg agctcgtgca ccttgccgtc
     2221 acgacggata gtcagacgca gccacttgct gagcgcattg acgcacgaca cgccaacacc
     2281 atgcaggccg ccggaaacct tgtagctgtt ctggttgaac ttgccgccgg cgtgcagttc
     2341 ggtcagtgcg atttcggccg ccgagcgcct gggctcgtgc ttatcgtcga acttgatgcc
     2401 ggtcggaatg ccgcggccat tgtcgacgac tgaaatggag ttatcggtgt ggatggtgac
     2461 gacgatgtcg tcacagtggc cggccagcgc atcgtcgatc gcgttgtcga cgacctcgaa
     2521 caccatgtgg tgcaggccgg tgccgtcctg cgtgtcgccg atgtacatgc ccggccgctt
     2581 gcgcaccgcc tccagcccct cgaggatctg gatgctgctt tcgccgtagg ggtcgatcgg
     2641 cttcggcagg ttatcggggc tgttcggaat atcgctcatc gctgttcttg cactctggaa
     2701 aaacgcagaa gcg
//
LOCUS       DATIRT010000184         5666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1107761, whole genome shotgun sequence.
ACCESSION   DATIRT010000184 DATIRT010000000
VERSION     DATIRT010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107761"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..1530
                     /gene="soxC"
                     /locus_tag="VLN25_04355"
     CDS             187..1530
                     /gene="soxC"
                     /locus_tag="VLN25_04355"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HSQ09834.1"
                     /translation="MSKGERAVVPGRIVAAPESHLTAEMLRGAARSRRGFLQGGSALA
                     GAVAAVAGGRAGAQEKAPFEPPNVPSWTRSLGAPVVAKPYGTPSEFEANVIRRQSPGL
                     TPQPQSSISFTPLQSLFGIITPSGVHFERHHSGMPVVNPTQHRLMIHGLVKRPLILTM
                     DDILRFPSVSRIHFIECGANSGMEWANVAVPTVQYTHGMLACSEFTGVLLSTLLEEVG
                     YDKKNAKFVLAEGADGSALTRSIPMEFALSDVMVVYGQNGEMLRPENGYPLRLVVPGV
                     QGVSSVKWLRRLEVGDKPWNAREESLHYVDLLPDGTHRQYSIVQEVKSVITAPSGGQK
                     LLGKGYYEITGFAWSGRGKVRRVDVSTDGGRNWGQARLQEPVLTKALARFRFDWTWNG
                     GPALLQSRAVDETGHIQPALPQLRAVRGTRSIYHNNGIQTWQVNPNGEVWNVQVG"
     gene            1514..2590
                     /locus_tag="VLN25_04360"
     CDS             1514..2590
                     /locus_tag="VLN25_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSQ09835.1"
                     /translation="MFRWVDRARLVIVLAGLSAAAAGAQSTGSSDGARFGFGRPASAQ
                     EIAGWDIDVRPDGHGVKKGRGTVAQGQEIYDAQCASCHGTFGESNRYMAVAGGVKKED
                     LAAGRASALTRPDGIRTLGTKLNAATTLWDYIYRAMPWTNPQSLTADQVYAVSAYVLH
                     LNEIVPADFELNDGNLTKVQMPNRNGMTTNHGMWSVKGKPDVQGSNCVKDCVSEVKVV
                     SELPEFARNQHGNLAEQKRPIGPFRGIDTTRYDAGKPGAVATATGAVPVAAAAATGPN
                     VKDLLTRNACTACHATDSKLVGPSFREVGTKYQARSDAEAYLAKKIKEGGQGAWGGVP
                     MPAQPGLKEDEAKVLARWILAGAK"
     gene            2679..3146
                     /gene="soxY"
                     /locus_tag="VLN25_04365"
     CDS             2679..3146
                     /gene="soxY"
                     /locus_tag="VLN25_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HSQ09836.1"
                     /translation="MDGQRREFVRAGGVLGLMVAAGLLTAKEAQAQQAAWNKAAFEAK
                     NLDETVKALGGTSAAQSGDISINAPDIAENGAVVPVGVSSKIPNTQAVYILVEKNPNS
                     LAAGFGIPAGTEANVATRIKMGQTSNVHAVVKADNKFYVATKEVKVTLGGCGG"
     gene            3179..3490
                     /gene="soxZ"
                     /locus_tag="VLN25_04370"
     CDS             3179..3490
                     /gene="soxZ"
                     /locus_tag="VLN25_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HSQ09837.1"
                     /translation="MPDPMRIRATEQPDGVDVRVLMSHEMESGQRRDSAGNLVPAHFI
                     QTVTVTSAGKPVLTADWGPAVSKNPYLQFKFKGAKKGDKLTVTWLDNKGETRTDEATI
                     N"
     gene            3637..4455
                     /gene="soxA"
                     /locus_tag="VLN25_04375"
     CDS             3637..4455
                     /gene="soxA"
                     /locus_tag="VLN25_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HSQ09838.1"
                     /translation="MKKQTLATIVAAVVLGGASASALAQGSAAEGIARYREMLQDGNP
                     AELTVARGEEIWKTKRGPKNVSLEQCDLGIGPGVIKGAYAQLPRYFADADQVMDFESR
                     VVWCMVNLQGMDHAAITKSPYSGAGQRQTDIEALAAFGAEMSRGIPVAIPQSHPKERA
                     SFDRGKQAFYMRGGPYDFSCATCHGVTGQRIRLQDLPNISSNKPDANRAFTTWPAYRV
                     SQGALRSMQWRLYDCFRQQRWPELVFLSPTSIDLITFLGVNANGAKMDAPAIKR"
     gene            4466..5083
                     /gene="soxX"
                     /locus_tag="VLN25_04380"
     CDS             4466..5083
                     /gene="soxX"
                     /locus_tag="VLN25_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HSQ09839.1"
                     /translation="MKKKVIFSITGLAVLAGCATYVAPTKEESVQAVKSSFKERGPAK
                     LDRLNQTAIQAICSDAQVMGKEVPKAEAEAMQKKVLATIKYPADGKFLGDYKQGERIA
                     QSGVGMQWSDGPTTVNGGNCYACHQISDAEVSFGNIGPSLKGYGKLRGYNEATLKYTW
                     GKIWNTHAFSLCSQMPRYGDAGILSEAQIKDVMALLLDPNSPVNK"
     gene            5136..5627
                     /locus_tag="VLN25_04385"
     CDS             5136..5627
                     /locus_tag="VLN25_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HSQ09840.1"
                     /translation="MHRRAVMSALAAALASAAIPSRADVRAPIRWNDIRLVDGRTLKA
                     SELQGRTVVVEFWASWCPFCMKQNPFIEKLHRQAGGDSLRVLTFTIDKTEQPARDYLA
                     KHGYTFAAAMAGPQSEAWFGRRRGLPEVHVVDPRGRIVFSEIGEMFEEDIAALARFAA
                     RSP"
BASE COUNT         1129 a         1697 c         1912 g          928 t
ORIGIN      
        1 gacggagcga tggtgtacta ctcgatcagc gatcctaatg tcgtcacgtt gtgtcgcacc
       61 gcctgcgtgc acatcgcggg tcagcaggac gatcactcgg tcacgagtcg cacgctgaag
      121 cgattcatgc cggcaaagta gcggctgccg gtgcgtcgaa actagaagaa gagcatggga
      181 gtgcagatga gtaaaggaga acgagccgtg gtgcccgggc gcatcgtcgc ggcgcccgaa
      241 agccacctca cggccgagat gctgcgaggc gcggcgcgat cgcgccgtgg attcctccag
      301 ggcggctccg cattggcagg ggccgtcgcg gcggtcgcag gcgggcgggc aggtgcgcag
      361 gaaaaagcgc ccttcgagcc gcccaatgtt ccgtcgtgga cacgatctct cggtgcaccc
      421 gtcgtcgcca agccgtatgg cacgccgtcg gagttcgagg ccaacgtcat ccgccggcaa
      481 agccccggcc tgacgccgca gccgcagtcg tcgatcagct tcacgccgct gcagagcctg
      541 ttcggcatca tcacgccaag cggggtgcac ttcgagcgcc accactccgg catgccggtc
      601 gtcaatccga cccagcaccg cctgatgatc cacgggttgg tcaagcggcc gctgattctc
      661 acgatggacg acatcctgcg atttccgtca gtgtcgcgga ttcatttcat cgagtgcggc
      721 gcgaactcgg gcatggagtg ggccaatgtt gccgtgccca cggtgcagta cacgcacggc
      781 atgcttgcct gcagcgagtt caccggcgta ttgctctcga cattgctcga agaagtcgga
      841 tacgacaaga agaatgcaaa gttcgtgctg gccgaggggg ccgatggctc cgcgctgaca
      901 cgctcgattc caatggagtt cgcgctcagc gacgtcatgg tggtctacgg ccagaacggc
      961 gaaatgctgc gtcccgagaa tggctatccg ctgcgcctcg tcgtgcccgg cgttcagggt
     1021 gtgtcgagcg tcaagtggct gcgtcgcctc gaagtcggcg acaagccctg gaatgcacgc
     1081 gaggaatccc ttcactacgt cgacctgctg cccgatggga cgcaccgcca gtattcgatc
     1141 gtgcaggaag tgaagtcggt gatcacggcg ccgtcgggcg gccagaaact gctgggcaag
     1201 ggctactacg aaatcaccgg gttcgcgtgg agcgggcgcg gcaaggtgcg tcgcgtcgac
     1261 gtgtcgaccg atggtggccg caactggggg caggcgcgcc tgcaggagcc ggtgctgacc
     1321 aaagcgttgg cgcggtttcg tttcgactgg acatggaacg gcggcccggc actgttgcag
     1381 agccgcgccg ttgacgagac cgggcacata cagccggccc tgccgcagct gcgtgcggtc
     1441 cgtggtacgc gttcgatcta tcacaacaac gggatacaga cctggcaggt caacccgaac
     1501 ggggaggtgt ggaatgttca ggtgggttga tcgcgcgcgc ctcgtcatcg tgctggcggg
     1561 cctgtctgcg gccgctgcag gtgcgcagtc gacaggcagc tccgacggag cccgcttcgg
     1621 attcggtcga ccggcaagcg ctcaggagat cgcgggctgg gacatcgacg tgcgccccga
     1681 cggtcacggc gtgaagaagg gccgtggaac ggtcgcacag gggcaggaaa tctatgacgc
     1741 ccagtgtgcg agctgtcacg gaaccttcgg cgaaagcaac cgttacatgg ctgtcgccgg
     1801 gggcgtgaag aaagaggatc tcgcggcggg ccgcgccagt gcactgaccc ggcccgacgg
     1861 catccgtacg ctcggtacca agctgaacgc ggcgaccacg ctgtgggatt acatctatcg
     1921 cgccatgccg tggaccaatc cgcagtcgct gacggcagac caggtctacg ctgtctcggc
     1981 ctacgtgctg cacctgaacg agatcgtccc ggcagatttc gagctgaacg acgggaacct
     2041 taccaaggtg cagatgccga accgcaacgg catgacgacc aatcacggca tgtggtcggt
     2101 taagggcaag cccgatgtgc agggttcgaa ctgcgtgaaa gactgtgtct ctgaggtcaa
     2161 ggtcgtctcg gagcttccgg agttcgcacg caaccagcat ggcaacctgg ctgagcagaa
     2221 gcgtccgatt ggccccttcc gcgggatcga tacgacccgt tacgatgccg gcaagccggg
     2281 ggcagtggca acggcaacgg gagcggttcc cgtggctgcc gcggccgcga cgggaccgaa
     2341 tgtgaaggac ctgctgacgc gcaatgcatg cacggcgtgt cacgcgaccg actccaagtt
     2401 ggtggggccg tcgttccgcg aggtgggcac gaagtaccag gcacgcagtg atgccgaggc
     2461 ctatctcgcc aagaagatca aggaaggcgg gcagggtgca tggggtggtg tcccgatgcc
     2521 ggcgcagccc ggcctcaagg aagacgaggc gaaggtgctg gcacgctgga ttctggcggg
     2581 tgccaagtaa gtggtaacag caatggggga cgtcggggcg acacccggtc gtttcattta
     2641 aagtagcgag caatttttct tcggagcagg agactcagat ggatggtcaa cgcagggagt
     2701 tcgtcagggc cggcggcgtg ctgggcttga tggtggcggc gggcttgctg accgccaagg
     2761 aagcgcaggc acagcaggcc gcgtggaaca aggcggcatt cgaggccaag aacctcgacg
     2821 agacagtcaa ggcgctcggc ggtacatcgg ccgctcaaag tggtgacatc tcgatcaacg
     2881 cgcctgatat cgccgaaaac ggtgcggtcg tgccagtcgg cgtgagcagc aagatcccca
     2941 acacccaggc ggtctacatc ctggttgaga agaatccgaa ttccctggca gcaggattcg
     3001 gcattccagc gggcaccgaa gccaatgtcg cgacccgcat caagatgggc cagacgtcga
     3061 acgtgcacgc ggtggtgaag gcagacaata agttctacgt cgccaccaag gaagtcaagg
     3121 tcacgctcgg cggctgcggc ggctagcaca ttcacttttc tgatttgagg agcagcaaat
     3181 gccagatccg atgcgtatcc gcgcaaccga gcagcctgac ggcgtcgacg tgcgcgtatt
     3241 gatgagccat gagatggagt ccggacagcg ccgcgattca gcgggcaacc tggtgccggc
     3301 gcatttcatt cagacggtga ccgtgacgtc cgccggcaag ccggtgctga ctgcggactg
     3361 gggcccggcg gtgtcgaaga atccgtacct ccagttcaag ttcaagggtg ccaagaaggg
     3421 tgacaagctc accgtaacct ggctcgacaa caagggcgag acccgcaccg acgaagcgac
     3481 gatcaactga cgtaccgggg tcacaggggc cgcaagcctg caggtgcgcg cggtgtatga
     3541 ctgcatccgg ccgtaagccg gtgaccacga gttcagtgat agagctcccg cagaggagca
     3601 aaacgggaag aaacttcagc caggaggcag aaacacatga agaagcagac gttggccacg
     3661 atcgtggccg cggtggttct gggcggtgcg agcgccagtg cgctggccca gggcagtgcc
     3721 gcggagggca tcgcgcgcta tcgcgaaatg ctgcaggatg gcaatcccgc cgagctcacg
     3781 gtcgcacgtg gcgaggagat ctggaagacc aagcgtgggc cgaagaacgt gtcgctcgag
     3841 cagtgcgacc tcggcatcgg tcccggcgtc atcaagggcg cctatgcgca gctgccacga
     3901 tactttgccg atgccgacca ggtgatggat tttgaatcgc gcgtggtctg gtgcatggtc
     3961 aacctgcagg gcatggatca cgcggcgatc accaagagcc cgtactcggg cgccggacag
     4021 cggcagaccg acatcgaggc gctggctgca ttcggggccg agatgtcgcg cggcatcccg
     4081 gtcgccattc cgcagagcca tcccaaggaa cgggcgtcgt tcgatcgcgg caagcaggcg
     4141 ttctacatgc gcggcggacc gtatgacttt tcctgcgcca cctgccacgg cgtgaccggg
     4201 cagcgcattc gcttgcagga cctgccgaac atttccagca acaaaccgga cgccaatcgc
     4261 gcgttcacga cctggccggc ataccgggtc tcgcaaggcg cactgcgctc gatgcagtgg
     4321 cgtctttatg actgcttccg gcagcagcgc tggccagagc tggtgttcct ttcaccgacc
     4381 tcgatcgacc tgatcacctt ccttggcgtc aatgccaacg gcgcgaagat ggacgcgccg
     4441 gccatcaagc gctgaggagc cgacgatgaa gaagaaagtc attttctcga tcaccgggct
     4501 cgcggttctg gcgggctgcg ctacgtacgt ggcgccgacg aaggaagaat cggtccaggc
     4561 ggtcaagtcc tcgttcaagg aacgagggcc ggccaagctc gaccggctca accagaccgc
     4621 gatccaggcc atctgttccg atgcccaggt aatgggcaag gaagtgccga aggccgaggc
     4681 cgaggcgatg cagaagaagg tcctggcaac gatcaagtat ccggccgacg gcaagttcct
     4741 cggtgattac aagcagggcg agcgcatcgc gcagtccggt gtgggcatgc aatggagcga
     4801 cggtcccacc acggtcaacg ggggcaactg ctatgcctgc catcagatca gtgacgccga
     4861 agtttcgttc gggaacatcg gtccgtcgct gaagggttac ggcaagctgc gcggatacaa
     4921 cgaggcgacc ctgaagtaca cgtggggcaa gatctggaac acgcacgcgt tcagcctgtg
     4981 ctcgcagatg ccgcgctacg gggatgccgg catcctgagc gaggcgcaga taaaggacgt
     5041 catggcgctg ctgctggacc cgaactcgcc cgtcaacaag taggtcccct ggcccggccg
     5101 ccagcggcag ccgggcttct tcttcagaga ttcccatgca tcgccgtgct gttatgtccg
     5161 cgctggctgc cgcgcttgcc agtgcggcga ttccctcgcg cgccgacgtt cgtgcgccga
     5221 tccgctggaa cgacatccgg cttgtcgacg gtcgcacgct caaggcgagc gaactgcagg
     5281 gccggaccgt ggtggtcgaa ttctgggcca gctggtgtcc gttctgcatg aaacagaacc
     5341 cgttcatcga gaagctgcat cgccaggcgg gaggcgacag ccttcgcgtg ctgaccttca
     5401 ccatcgacaa gaccgagcag cctgcgcgcg attacctggc caagcacggc tacaccttcg
     5461 cggcggccat ggcaggaccg cagtcggaag catggttcgg ccgccgacgg ggcctgccgg
     5521 aggtccatgt cgtcgatccg cgcggacgca tcgtcttcag cgagatcggc gagatgttcg
     5581 aggaagacat cgcagcgctg gcgcgcttcg ccgccaggtc gccgtagttc gaacaagaca
     5641 ccagagaacg gacagggaga ggtacg
//
LOCUS       DATIRT010000185         7770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1501189, whole genome shotgun sequence.
ACCESSION   DATIRT010000185 DATIRT010000000
VERSION     DATIRT010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7770
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501189"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VLN25_04390"
     CDS             <1..597
                     /locus_tag="VLN25_04390"
                     /inference="COORDINATES: protein motif:HMM:NF022426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HSQ09841.1"
                     /translation="AVVVLAGCSSSMSKDQCAMADWHMIGFEDGLQGWSADRIGVHRV
                     ACAPHQVTPTLAAYMEGRQRGLQEFCQPKNGFRVGLRGGGYANVCSGPTEPAFVDSYR
                     YGRQIHDARTELRNTQSRLRSAREGLAYTEAGMASVTTELVLPVVPTDRRAFLATELI
                     RLTQERTELIARIDQSMARTQQLAVNVQELERHSPYAL"
     gene            complement(674..1084)
                     /locus_tag="VLN25_04395"
     CDS             complement(674..1084)
                     /locus_tag="VLN25_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase subunit alpha"
                     /protein_id="HSQ09842.1"
                     /translation="MIISTFDDLLRAAREQADSQRLLFVFAKADVPDDATPHQRERFE
                     AGLGGTLTPQMCVDKAPSELDSFAVLLDEARQVGQAWDIVFVAALSGTGTSGQIAAQT
                     QEALQRMVESIRAGAVGGFIPFDVHGDPVRVSND"
     gene            complement(1104..2249)
                     /locus_tag="VLN25_04400"
     CDS             complement(1104..2249)
                     /locus_tag="VLN25_04400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HSQ09843.1"
                     /translation="MRLSDCHNFHDFRELARKRLPGPIFNYIDGAADDEVTHRQNTAS
                     FEGCDLVPNVLRGVESVDLSVTVMGQKLAMPVYCSPTALQRLFHHQGERAVAAAASKH
                     GTLFGVSSLGTVSLEELRKEYATPQVYQFYFHKDRGLNRAMLERAKEAGVDVMMLTVD
                     SITGGNRERDLRTGFSIPFRLTLNGMFQFAVKPIWGLNYLLHERFSLPQLDSHVDMGG
                     GAMSIGRYFTEMLDPSMNWNDVEAMVRSWNGQFCLKGIMSVADARRAADIGCTGIVVS
                     NHGGRQLDGSRAPFDQLTEIVDAVGDRLDVMMDGGIQRGTHVLKALSLGAKAVGVGRF
                     YLFPLAAAGQAGVERALGLMRAEIERGMKLMGCSTILQLSRENMKLR"
     gene            complement(2303..5590)
                     /gene="icmF"
                     /locus_tag="VLN25_04405"
     CDS             complement(2303..5590)
                     /gene="icmF"
                     /locus_tag="VLN25_04405"
                     /EC_number="3.6.5.-"
                     /EC_number="5.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0047727 - isobutyryl-CoA mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fused isobutyryl-CoA mutase/GTPase IcmF"
                     /protein_id="HSQ09844.1"
                     /translation="MTDLSEIKKLASYRPKQKVRFVTAASLFDGHDASINIMRRILQS
                     MGAEVIHLGHNRSVEEIVTAAVQEDVQGIAVSSYQGGHVEYFKYMLDLLRERGGSAIR
                     VYGGGGGVIVPAEIRELHDYGIARIFSPEDGQKMGLQGMIGLMVAECDTDLSQHAPSA
                     LSALQDKDLTKRWRALAQAITALELGNADAKFREALHKAADIAKVPTLGITGTGGAGK
                     SSLTDELIRRLRLDQADRLNIAVISIDPSRRKSGGALLGDRIRMNAIGPWGVGVQRVY
                     MRSLATREAGSEISQALPDAIAACKVAGFDLVLVETSGIGQGDAAIVPHADATLYVMT
                     PEYGAASQLEKIDMLDFADFVAINKFDRKGAADALRDVSKQVQRNKGDFSKKPDEMMV
                     FGTQAARFNDDGVTALYQALLPKLRELGLKAGEGVLPKVATRHSTHQVPIVPPQRTRY
                     LAEISDTVRGYKKRSLEQSRIARQRQQLAEAGRMLAAECKIENADLGKLAAERDARLE
                     ARSKKLLEMWPQMQKAYAGDEYVVKIRDREVRTKLTSTSLSGSRIRKVALPHYEDHGE
                     LLRWLLLENVPGSFPYTAGVFAFKRENEDPTRMFAGEGDPFRTNKRFKLLSEGMPAKR
                     LSTAFDSVTLYGNDPAVRPDIYGKVGNSGVSIATLDDMKVLYSGFDLTAPNTSVSMTI
                     NGPAPTILAMFMNTAIDQNLDKFRADNGREPTDDEAAKIRQWVLENVRGTVQADILKE
                     DQGQNTCIFSTEFSLKVMGDIQQYFVHHNVRNFYSVSISVYHIAEAGANPISQLAFTL
                     SNGFTFVEAYLARGMHIDDFAPNLSFFFSNGMDPEYTVMGRVARRIWAVAMRDKYGAN
                     ERSQKLKYHCQTSGRSLHAQEIQFNDIRTTLQALIATYDNANSLHTNAFDEAITTPTE
                     ESVRRAMAIQMIINREWGLAKNENPNQGAFIIDELTELVEEAVLKEFESISERGGVLG
                     AMELGYQRGKIQEESLHYEHLKHTGEYPIIGVNTFRNPHGERVPDHIELARSTEDEKQ
                     SQLKRLQDFQKRHAKQAPAMLVRLQKAVIDNGNVFEVLIEAVRVCSLGQITNALFEVG
                     GQYRRSM"
     gene            complement(5824..6741)
                     /locus_tag="VLN25_04410"
     CDS             complement(5824..6741)
                     /locus_tag="VLN25_04410"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HSQ09845.1"
                     /translation="MELYQLRTFAAVAEEGHLTRAAERLHLSQPAVSGHIKALEQELD
                     VRLFERAATGMVLTEPGRQLLAHAQKVLSAAEGVKRAAQQMHTEVTGLLHVGTVADPV
                     DNRLGAVLSATVHRYPLLRLELHQTMSGTALESVRDGRLDASFYFGDVPAAPLHALPL
                     RQLIYRVAAPAAWGARVLDARWPDIAAMPWIRTPTLSTHTHLVTQLFAAHGIEAPEPA
                     VLADDETVITNLISSGLGVALVRDEVARARAADGDLAIWPGAAIETTLWFVCPASRIE
                     EPALAALLTLVRETWNLGELASQPRPATA"
     gene            6905..7276
                     /locus_tag="VLN25_04415"
     CDS             6905..7276
                     /locus_tag="VLN25_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09846.1"
                     /translation="MELLMSVLTRLFPWPPGFLPRRAAAVAISAPSRPEARGAVETPA
                     TDAVAHIGTQPVAGSPSLEERRAHALRSMFPMLFSMVARRSDLWRAIAIERYLAQATN
                     IADLEQRIHEVQRQRHFSWSE"
     gene            7513..7701
                     /locus_tag="VLN25_04420"
     CDS             7513..7701
                     /locus_tag="VLN25_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09847.1"
                     /translation="MEKSLGHTKQQNPLTRFFEWLTESFERAQMRDHDRYVAAASNPR
                     DVSQRLHRIEQGEESFHA"
BASE COUNT         1346 a         2491 c         2435 g         1498 t
ORIGIN      
        1 gccgtcgtgg tgctggccgg ctgctccagc agcatgagca aagaccagtg tgcgatggcg
       61 gactggcaca tgatcggctt cgaggatgga ctacagggct ggtccgctga ccggatcggc
      121 gtgcatcgcg tcgcctgcgc accgcatcag gtcacgccga ccctcgccgc ctacatggaa
      181 ggacgacagc gcggactgca ggaattctgt cagcccaaga acggctttcg cgtcggcctg
      241 cgcggcggcg gctatgccaa cgtgtgctcg ggcccgaccg agcccgcgtt cgtcgacagc
      301 taccgctacg gccgtcaaat tcacgatgcg cgcaccgaac tgcgcaacac gcagtcacgg
      361 ctgcgcagcg cccgcgaagg tctggcctac accgaagcgg gcatggcgag cgtcaccacg
      421 gaactcgtgt tgcccgtcgt accgaccgac cgacgcgcct tcctggcgac ggagttgatc
      481 cggctgacac aggaacgcac cgagctgatt gcgcgcatcg atcagtcgat ggcgcgaacg
      541 cagcagctcg cggtcaacgt acaggaactc gagcgccaca gtccgtacgc gctctgactt
      601 tcactgggtt cggagttgtt ggacgccgcg acccactatc gtggctggcc caccaagact
      661 ggccctgacg gtcctagtcg ttgctcaccc gaaccggatc accgtggacg tcgaacggaa
      721 tgaatccacc cacggcgccg gctcgaattg attcgaccat gcgctgcagc gcttcctgcg
      781 tctgtgcggc aatttggccg gatgtacccg tgccggacag ggcggcgaca aacacgatgt
      841 cccacgcctg gccaacctga cgcgcttcat cgagcagcac ggcgaacgaa tccagctcgc
      901 tgggagcctt gtccacgcac atctgcggcg tcaacgtgcc gcccagcccg gcttcaaagc
      961 gttcgcgttg atgaggcgtg gcgtcgtccg gaacgtcagc cttggcgaat acgaacagca
     1021 gtcgctgaga gtccgcctgt tcccgcgccg cgcgcaggag gtcgtcaaag gtcgaaatga
     1081 tcatgcgatc ccgtgtgtgc gacctatctc agcttcatgt tttcacgcga gagctggagg
     1141 atcgtcgagc agcccatcag cttcatccca cgctcgattt ccgctctcat cagacccagc
     1201 gcccgctcga cgccagcctg gccggcagcc gccagcggaa acagatagaa gcgtcctacg
     1261 ccgacggcct tggctccgag cgaaagggcc ttgaggacgt gcgtgccgcg ctggatgcct
     1321 ccgtccatca tcacatcaag acgatcaccg acggcatcga cgatttcggt cagttgatcg
     1381 aagggagcgc gcgagccatc gagttgtcga ccgccgtgat tggagacgac gatgccggtg
     1441 cagccgatgt cggcggcccg cctagcgtcg gccaccgaca tgatgccctt caggcagaac
     1501 tggccgttcc atgatcggac catggcttcc acgtcgttcc agttcatgga cgggtccagc
     1561 atctccgtga agtagcggcc aatcgacatg gcacccccac ccatatcgac gtggctgtcc
     1621 aactggggca ggctgaagcg ctcgtggagc agatagttca aaccccagat cggcttgact
     1681 gcaaactgaa acattccgtt cagtgtcagc ctgaatggaa tggaaaatcc ggtgcgcagg
     1741 tcgcgctcgc ggtttcctcc ggtgatgctg tccacggtca gcatcatcac gtcgactccg
     1801 gcctccttgg cacgttcgag catcgcgcgg ttgaggccgc ggtccttgtg aaagtagaac
     1861 tgatagacct gcggagtggc gtactccttg cgaagttctt cgaggctgac cgtgcccagc
     1921 gatgacacgc cgaacagggt tccgtgcttc gaagctgcgg ctgccactgc ccgttcgccc
     1981 tgatggtgga acagtcgctg gagcgcggtt ggggagcagt agaccggcat ggcaagtttc
     2041 tggcccatca cagtcacgga aagatcgacc gactcgactc cgcgcagcac attgggaacg
     2101 agatcacagc cctcgaagct tgcggtgttc tgccgatgag tcacctcatc gtccgccgcg
     2161 ccgtcgatgt aattgaagat cggacccggc agccgtttcc tggccagttc ccggaagtca
     2221 tgaaaattgt gacagtcgct taggcgcatg tcggaatgga gtcgtcgcct ttcgatggcg
     2281 acagccttgg ccggaaacgg agctacatgc tccgccggta ctgcccccca acctcgaaca
     2341 acgcattcgt gatctgtccg agcgaacaga cacgcaccgc ttcgatcagc acctcgaaca
     2401 cgttgccgtt gtcgatcacc gctttctgca gccgcacgag catcgccggc gcctgcttcg
     2461 catggcgctt ctgaaagtcc tgcaggcgct tcaactggct ctgtttttca tcctcggtcg
     2521 agcgggcgag ttcgatgtgg tcgggcacgc gctcgccatg cggattgcgg aaggtgttga
     2581 cgccgatgat cgggtattca ccggtgtgct tcaggtgctc gtagtgcagt gactcttcct
     2641 ggatcttgcc gcgctgataa ccgagttcca tcgcgccgag cacgccgccg cgttcgctga
     2701 tggactcgaa ctctttcagc accgcttcct caacgagctc agtgagttcg tcgatgatga
     2761 acgcgccctg gttggggttc tcgttcttcg ccagacccca ttcgcggttg atgatcatct
     2821 ggatcgccat cgcccggcgc acgctttcct cggtcggcgt ggtgatcgct tcgtcgaagg
     2881 cgttggtgtg cagcgagttg gcgttgtcgt aggtcgcgat cagcgcctgc agcgtggtgc
     2941 ggatgtcgtt gaactggatt tcctgcgcgt gcagcgagcg gccgctggtc tggcagtggt
     3001 acttcagctt ctgggatctt tcattcgcgc cgtatttgtc gcgcatcgca accgcccaga
     3061 tacggcgcgc gacgcggccc atcacggtgt actcggggtc catgccattg ctgaagaaga
     3121 agctcaggtt cggtgcaaag tcgtcgatgt gcatgccccg cgccagatac gcctcgacga
     3181 atgtgaagcc atttgaaagc gtgaacgcga gctggctgat cgggttcgcc cccgcctcgg
     3241 cgatgtgata gacgctgatg ctcaccgaat aaaagttgcg cacgttgtga tggacgaagt
     3301 actgctggat gtcgcccatc accttgagcg agaactcggt cgagaagatg caggtgttct
     3361 ggccctggtc ttccttcagg atgtcggcct gcacggtgcc gcgtacgttc tccagcaccc
     3421 actggcggat cttcgcggct tcgtcgtcgg tcggctcgcg gccgttgtcg gctcggaact
     3481 tgtcgaggtt ctggtcgatc gcggtattca tgaacatcgc gaggatcgtc ggcgccgggc
     3541 cgttgatcgt catcgatacc gacgtgttgg gcgcggtcag gtcgaagccc gaatacagca
     3601 ccttcatgtc gtcaagcgtg gcgatgctga cgccggagtt tccgaccttg ccgtagatgt
     3661 cgggacgcac ggccgggtcg tttccgtaca gcgtcacgct gtcgaaagcc gttgagagcc
     3721 gcttggccgg catgccctcg gaaagcagct tgaagcgctt gttggttcgg aaaggatccc
     3781 cctcaccggc gaacatgcgc gtcggatcct cgttctcgcg cttgaaagca aatacgcccg
     3841 ccgtgtacgg gaaactgccc ggcacgtttt cgagcagcag ccaccgcagc aactcgccgt
     3901 ggtcctcata gtgcggcagc gcgaccttgc ggatcctgct gccggacagc gacgtgctcg
     3961 tgagcttggt ccggacctcg cggtcgcgga tcttcacgac gtactcgtcg cccgcatagg
     4021 ccttctgcat ctgcggccac atctcgagca gcttcttgct gcgggcctcg agcctcgcgt
     4081 cgcgctcagc ggcgagcttg ccaaggtccg cattctcaat cttgcattcg gcggccagca
     4141 tgcgacccgc ttcggccagc tgctgccgct ggcgcgcgat gcgcgactgc tcgagcgaac
     4201 gcttcttgta gccgcgcacg gtgtcggaaa tttcggccag gtagcgcgtg cgctgcggcg
     4261 gcacgatcgg cacctggtga gtcgaatggc gtgtcgccac cttcggcagc acgccctcgc
     4321 cggccttcag tcccagttcc ctcagcttcg gcagcagcgc ctggtaaagc gcggtgacgc
     4381 cgtcgtcgtt gaagcgcgcg gcctgcgtgc cgaacaccat catctcgtcg ggcttcttcg
     4441 aaaagtcgcc cttgttgcgc tggacctgct tggacacatc gcgcagcgcg tcggccgcgc
     4501 ccttgcgatc gaacttgttg atcgcgacga aatcggcgaa gtccagcatg tcgatctttt
     4561 cgagctggct ggcggcaccg tactccggcg tcatcacgta cagcgtcgcg tcggcgtgcg
     4621 gcacgatcgc ggcatctccc tgcccgatgc ccgaggtctc gaccagcacg aggtcgaagc
     4681 ccgcgacctt gcaggcggca atcgcgtcgg gcagtgcctg cgaaatctcg gaaccggctt
     4741 cgcgtgtcgc gagcgaacgc atgtagacac gctggacccc cacgccccag ggaccgatcg
     4801 cattcatgcg gatgcggtca ccgagcagcg cgccgccgct ctttcggcgc gacgggtcaa
     4861 tcgagatcac ggcgatgttc agcctgtctg cctggtcgag ccggaggcgg cggatcaact
     4921 cgtcggtcag gctcgacttg ccggcacccc cggtgccggt gatgccgagt gttggcacct
     4981 tggcgatgtc ggcggctttg tgcagcgcct cgcggaattt cgcgtctgca ttgccaagtt
     5041 cgagcgcggt gatggcttgc gccagtgcac gccagcgttt ggtcaggtcc ttgtcctgca
     5101 gcgccgacag cgcgctaggt gcgtgctgcg aaagatcggt gtcgcactcg gcgaccatca
     5161 gcccgatcat tccctgcagg cccattttct gcccgtcctc gggcgagaag atgcgtgcga
     5221 taccgtagtc gtgcagttcc ctgatctcgg ccggaacgat gacgccgccg ccgccgccgt
     5281 agacacggat ggccgagccg ccgcgctcgc gcagcaggtc gagcatgtac ttgaagtact
     5341 cgacgtggcc gccctgataa ctcgacacag cgatgccctg cacgtcctcc tgcactgcgg
     5401 cggtgacgat ttcctcgacc gagcggttat gacccagatg gatcacctcc gcgcccatgc
     5461 tctgcaggat gcgacgcatg atgttgatgc tggcgtcgtg gccgtcgaaa aggctcgctg
     5521 cagtgacgaa acgcaccttc tgcttcgggc ggtaactggc gagcttcttg atttccgaaa
     5581 gatcggtcat gcgcgctcct cgacggtcgg ccggccgctg gaagacgaat catcgccgcg
     5641 cgccgggtcg acagcaatcg cggacggaga gggggacgga acggcgctaa cgctagctgc
     5701 cgttgacgtt aacgtcaacc tcagcggagg gagggtgccg gtgtgcccgg tcattcccgg
     5761 aagcgggtgc cgttccgtga cactggtttt cgagccgagg cttctcggcc ggtcgagttg
     5821 cggtcaggct gtggccggtc ggggctggct ggcaagctcg cccagattcc aggtttctct
     5881 aaccaatgtg agcagtgcgg ctagcgcggg ctcttcgatc cgtgaggcgg ggcacacgaa
     5941 ccaaagcgtc gtctcgatcg cggcgccggg ccagatcgcc agatcgccat cggccgcacg
     6001 ggcgcgcgcc acttcatcgc gcacaagggc gaccccgagg cccgatgaga tgaggttcgt
     6061 aatgacggtt tcgtcgtccg cgagcacggc gggctccggc gcctcgatcc catgagcggc
     6121 gaagagctgt gtgacgaggt gcgtatgcgt gctcagcgtc ggtgtccgga tccacggcat
     6181 cgccgcgata tcaggccagc gcgcatcgag cacgcgcgcg ccccaggcag ccggggcggc
     6241 cacgcgatag atcaactggc gcagcggcag cgcgtgcaga ggcgccgccg gcacgtcacc
     6301 gaagtaaaag ctcgcgtcga gccggccatc gcgcacgctt tcgagcgcgg tcccggacat
     6361 ggtctgatga agttcgagac gcagcagcgg gtatcgatgc acggtcgccg acagcacggc
     6421 gcccaaccgg ttgtcgaccg gatcggccac cgtgccgaca tggagcagcc cggtgacttc
     6481 ggtgtgcatc tgctgcgcgg cgcgcttgac gccttcggcg gcactcagca ccttctgcgc
     6541 gtgcgccagc aactgccgcc cgggctcggt cagcaccatg cccgtggcag cgcgttcgaa
     6601 caggcgaacg tcgagttcct gctccagtgc cttgatatgg ccgctgacgg ccggttggct
     6661 caggtgcagg cgctccgcgg cccgtgtcag gtggccttcc tcggccacgg cagcaaacgt
     6721 ccgtagttgg taaagctcca taaggcacac tccaaacgca gcctaatcgg gttcttgcac
     6781 tgccgcaata ccgtaacacc tccaatcgcg gcatctgatg ctaattatct gaatttacga
     6841 ttgtatttct gttgatattg ccccgattat gggttgtgcg aagcagcaat tcgcccctca
     6901 ccgattggag ctgctcatgt ccgtactgac ccgattgttt ccgtggccac cgggctttct
     6961 accccggcgc gccgcggcgg tggcgatttc cgccccgtcc cggccggaag cccggggcgc
     7021 tgtcgaaacg cccgcgaccg atgccgtcgc tcacatcggg acgcaacctg tcgctggatc
     7081 gccttcgctg gaggagcgcc gcgcccacgc gttgcgcagc atgttcccca tgctcttcag
     7141 catggtcgcg cgccgctcgg acctgtggcg ggccatcgca atcgagcgct acctggcgca
     7201 agcgacgaac atcgccgatc tcgagcagcg tatccacgag gtgcagcgcc agcgccactt
     7261 ctcgtggagc gagtgacgca cgccatgcgc gggcttgacg agtcgcaatc gtccgccgct
     7321 ttaggcggct ggcctagcga ctgttgcgca cgagtggcgc accagagcag caaaggactc
     7381 gcactgactg cggttgattg cgaaacacga tgccgcgcat ctgcattctc gattttcaat
     7441 tcggtgacga tcggctcatg atgaattcgt taaagagcac cgatcctcgc cagcgcgagt
     7501 gaaaggacga cgatggaaaa gagtcttgga catacgaagc agcagaaccc gctgacacgt
     7561 ttcttcgagt ggctgaccga gtcattcgag cgtgcgcaga tgcgcgatca cgatcgttat
     7621 gtcgctgcgg ccagcaaccc gcgcgacgtt tcgcagcgcc tacacaggat tgagcaaggc
     7681 gaggaaagct tccacgcctg aacacggttg tctccttggc gcgtccgcgt ctgtctccct
     7741 tgtacgcgcc cctcccaccg acgcggttca
//
LOCUS       DATIRT010000186         2433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_222955, whole genome shotgun sequence.
ACCESSION   DATIRT010000186 DATIRT010000000
VERSION     DATIRT010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222955"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1359)
                     /locus_tag="VLN25_04425"
     CDS             complement(<1..1359)
                     /locus_tag="VLN25_04425"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HSQ09848.1"
                     /translation="MEFTPNTDAPRPAGDLPYVTAAQASALMKSAMAISNELVATAFP
                     DITKAIQEELTGTWDFDVSSPLVQRLLSSKSDDLHRSFVEHLKQAQDQTFHEITVGRV
                     QPPPGSLDAETLSLVDSISVESNTVVERHASKVAGFADLALRDLNLVVAFVLGRSAVR
                     NSENPLGPNVYVRALLRAAEDCELHKEAWEFLLTRFEKPLGEELGQIVRQLLEHFANH
                     GVDVRSIRRSLAVPKGTTGGWQPPGVSGDGKPGEWSGSAQAPGQSGTGAGRPGRPGSV
                     AAAEGGPTRAPTAPDEPTQLLGGLLTRLQSNARGSRLPQLPSLGPASPELLQSLGEMQ
                     RDGVEQFVETGDFAMMPPMQSITSMRDELTSKSTRTVDKLTIELVGMLFDHVMQDKQV
                     PAEIKARLSRLQFPVLKAALMDAAFFASSAHPARKLIDRIAGTSAGWEPYGDDNQKYL
                     KEV"
     gene            complement(1478..2245)
                     /locus_tag="VLN25_04430"
     CDS             complement(1478..2245)
                     /locus_tag="VLN25_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HSQ09849.1"
                     /translation="MRRPSRRSPRTRVTLSEEDGVRYLHFGTEWIQGGMRIARPWQLE
                     LEYQKQMMMVALFIAEPPRILQLGLGAAALTKFCYRHLPAAHVTAVELSAEVIDTARQ
                     WFALPPDGSRLDVVEADAQDYLGEVRRADRADWLQVDLYDAAARGPVHDDAGFYRMCR
                     RALRRPGVACFNLFGSRFDPSFNAICTAFDDRVLALPAVDEGNRIVFALTGPRIDEPM
                     TAVARRAGALEVAWRLPFRRWLADLMQSNDLVDRLRV"
     gene            complement(2247..>2433)
                     /locus_tag="VLN25_04435"
     CDS             complement(2247..>2433)
                     /locus_tag="VLN25_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09850.1"
                     /translation="PRRNFYLFTSAETGKAHSLAPLVLRGYIRAGRIRTAESAPLFER
                     VVGDMMRDLGAPAAQPA"
BASE COUNT          426 a          806 c          794 g          407 t
ORIGIN      
        1 gacttccttc aggtacttct ggttgtcgtc accgtagggt tcccaacccg ccgacgttcc
       61 tgcgatacgg tcgatcagct tgcgcgcggg gtgcgcactc gaggcaaaga acgccgcgtc
      121 catcagcgcg gccttcagca ctgggaactg caggcgcgaa agccgcgcct tgatctcggc
      181 cggcacctgc ttgtcctgca tgacgtggtc gaacagcatg ccgaccagtt cgatcgtcag
      241 cttgtcgacg gtgcgggtcg acttcgatgt cagctcgtcg cgcatcgaag tgatcgactg
      301 catcggcggc atcatcgcga agtcgccggt ctcgacgaac tgctcgacgc cgtcgcgctg
      361 catttcgccg agcgattgca gcagttcggg cgatgccggg ccgagcgagg gcagttgcgg
      421 cagccgcgaa ccgcgcgcgt tcgactgcag acgcgtcagc agtccgccca gcaattgagt
      481 cggctcatcg ggggccgtcg gcgcacgagt aggaccgccc tcggcggccg ccacggagcc
      541 cggacgcccc gggcgtcctg ccccggtccc cgactgtccg ggtgcctgag cactgccgct
      601 ccactcgccg ggcttcccgt cacccgagac acccggcggt tgccagcctc cggtcgtgcc
      661 cttcggcacc gcgaggctgc gccggatcga tcgcacgtcg acgccatggt tggcgaagtg
      721 ttcgagcaac tgccgcacga tctgaccgag ttcctcgccc agcggcttct cgaagcgcgt
      781 cagcaggaac tcccacgctt ccttgtgcag ctcgcagtct tcggccgcac ggagcaatgc
      841 gcgtacatag acattcggcc cgagcggatt ctcggaatta cgcaccgcgc tgcggccaag
      901 cacgaaggcc acaacgagat tgagatcgcg cagcgccaga tctgcaaagc cggcaacctt
      961 gctggcgtgc cgctcgacca cggtgttgct ttcgaccgag atcgaatcga ccagcgacag
     1021 ggtttctgcg tcgagcgagc ccggtggcgg ttgcacccgg cccacggtga tctcgtgaaa
     1081 ggtctggtcc tgggcttgct tcaggtgctc gacaaacgag cggtgcaggt catccgactt
     1141 ggacgagagc agccgttgca ccagtggtga gctgacatcg aagtcccagg tcccggtgag
     1201 ttcttcctgg atggccttgg tgatgtccgg aaatgcagtg gcgaccaact cgttggaaat
     1261 cgccatcgcc gacttcatca gcgccgacgc ctgcgcagcc gtgacgtacg gcaaatcgcc
     1321 ggcagggcgg ggggcgtcgg tattgggggt gaactccatc gatgctcgta caaaggcctg
     1381 gccgtgcggc ccttaggaag gctccgactc tatccgggtg gggcccgctg tcaagttaca
     1441 tatgggcgcg agcaacccgc taattatgcg tgttggatca gacacgtaga cgatccacca
     1501 gatcattcga ctgcatcagg tccgccagcc atcgccggaa gggcaggcgc caggcgactt
     1561 ccagcgcgcc tgcacgccgc gccaccgccg tcatgggctc atcgatgcgg ggaccggtca
     1621 gcgcgaagac gatccgattg ccctcatcga ccgccggtag tgcgagcacg cggtcgtcga
     1681 acgcagtgca gatggcgttg aagctcggat cgaaccggct gccgaacaga ttgaaacagg
     1741 ccacacccgg acgccgcaac gcgcgccggc acatccggta aaagccggcg tcatcatgga
     1801 ccgggccgcg cgccgcggcg tcgtacaggt ccacctgcag ccagtcggcc cgatcggcgc
     1861 ggcgcacttc gccgaggtaa tcctgtgcgt ccgcttccac gacatcgagc cggctgccat
     1921 ccggcggcaa cgcgaaccac tgtcgtgcgg tgtcgatgac ctcggcgctc agttccaccg
     1981 ccgttacatg cgcggccggc aggtgccggt aacagaactt ggtcagcgcc gcggccccga
     2041 gaccgagctg aaggatgcga ggcggctcgg cgataaacaa ggccaccatc atcatctgct
     2101 tttggtactc gagctcgagt tgccagggac gcgcaatgcg cattccgccc tggatccatt
     2161 cggtgccgaa atgcaggtag cggacaccgt cctcttcgga cagcgtaacc cgggtgcgcg
     2221 gggagcgacg cgacgggcgt cgcacgtcag gcgggctgag cggcgggcgc tccgagatcg
     2281 cgcatcatgt ctccgaccac gcgctcgaac agcggtgcgc tttccgccgt gcggatgcgt
     2341 ccggcgcgga tatagccgcg caacaccagc ggcgccagcg aatgcgcctt gccggtctcg
     2401 gccgaggtga agagatagaa gttgcgacgc gga
//
LOCUS       DATIRT010000187         5010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1796438, whole genome shotgun sequence.
ACCESSION   DATIRT010000187 DATIRT010000000
VERSION     DATIRT010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1796438"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="VLN25_04440"
     CDS             complement(<1..951)
                     /locus_tag="VLN25_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSQ09851.1"
                     /translation="MNQPIPGGEADGKGDYGASSIQILEGLEAVRKRPGMYIGDTQDG
                     TGLHHMVFEVVDNAVDEALAGYCDDIVVTIHTDNSISVVDNGRGIPTGIKFDDKHEPK
                     RSAAEIALTELHAGGKFNQNSYKVSGGLHGVGVSCVNALSKWLRLTIRRDGKVHALEF
                     RQGEVQDRVIETLNSQEGPVEASPMKIAGATDKRGTEVHFLPDDAIFGVVDFHYEVLS
                     KRLRELSFLNNGVRIRLVDQRTGKEEDFAFSGGVKGFVQYINKTKTVLHPNAFYTAGE
                     AGGAASSSTAAVPIGVEVAMQWNDGYSESLLAFTNNIPQKD"
     gene            complement(1117..2217)
                     /gene="dnaN"
                     /locus_tag="VLN25_04445"
     CDS             complement(1117..2217)
                     /gene="dnaN"
                     /locus_tag="VLN25_04445"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HSQ09852.1"
                     /translation="MQLVKASRDALLKPLQVVSGIVERRQTLPILANILVRKDGERVS
                     FTATDLELQIQTSAPIGAGKDVAATTVAARKLVDILRALPESDVQLSLASKKLAVASG
                     KSRFNLQTLAAEEFPTVAQAEFTADFSLPAATLKYLLSMVHFAMAQQDIRYYLNGMLL
                     VVDGATVRAVATDGHRLALCEVQKDGASPKIEAIIPSKTILELSRLLPDNEDPVRVQM
                     AATQVKFSFGDIELISKLVEGKFPDYQRVVPAANTKTFVIGREDLMHALQRAAILTTD
                     KFKGVRLLLAAGALKINSTNADQEDAQEELEIDYAGDGLDIGFNVNYLLDVLLNVKSE
                     QLKFALGDALGSALMTLPDSEKFKYVVMPMRI"
     gene            complement(2454..3818)
                     /gene="dnaA"
                     /locus_tag="VLN25_04450"
     CDS             complement(2454..3818)
                     /gene="dnaA"
                     /locus_tag="VLN25_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HSQ09853.1"
                     /translation="MQHIWHDCSRLLESELTPQQYSAWIKPLKAIDFDESTSTLTIGA
                     PNRLKLDAIRAQFSDQIAYAASRIVQRPVAVVFAVENGAAVATLRFPAALASEVEVPT
                     LAEVAPPSPEHLERARLNANLTFDTFVTGKANQLARAAAMQVAENPGGSYNPLFLYGG
                     VGLGKTHLIHAVGNGILARKPDAKVRYIHAEQYVSDVVRAYQRKAFDDFKRYYHSLDL
                     LLIDDIQFFAGKARTQEEFFYAFEALVVGRKQIIITCDTYPKELKDMEDRLVSRFASG
                     LTVAIEPPELEMRVAILLKKAESEGVDMKEDVAFFIAKNLRSNVRELEGALRKVIAFS
                     SFHGRVVTVEVAREALKDLLSASTGQVTVELIQKTVADYYKIKVADMYSKRRPSNIAL
                     PRQVAMYLAKELTQKSLPEIGELFGGRDHTTVLHAVRKIGGERVKAGELNHALHVLEQ
                     TLKG"
     gene            4432..4572
                     /gene="rpmH"
                     /locus_tag="VLN25_04455"
     CDS             4432..4572
                     /gene="rpmH"
                     /locus_tag="VLN25_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HSQ09854.1"
                     /translation="MSTKRTYQPSKVKRARTHGFLVRMRSRGGRAVIAARRAKGRKRL
                     AV"
     gene            4713..>5010
                     /locus_tag="VLN25_04460"
     CDS             4713..>5010
                     /locus_tag="VLN25_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HSQ09855.1"
                     /translation="MLAADRAHSTRAAGKWLSMTAAWDPISPGCVRLGITVRKRMARR
                     AIDRALVKRIAREAFRHAVSGIERVAAGSGVGVDISVRLKRPVALPDDPQRPALT"
BASE COUNT          873 a         1547 c         1570 g         1020 t
ORIGIN      
        1 gtccttctgc gggatgttgt tggtgaacgc gagcaggctc tcgctgtagc cgtcgttcca
       61 ctgcattgcg acttcaacgc cgatcggcac cgccgccgtg ctgctgctgg ccgcacctcc
      121 ggcttccccg gccgtgtaga aggcgttcgg gtgcaggacc gtcttggtct tgttgatgta
      181 ctggacgaag cctttcacgc cgccggagaa cgcaaagtcc tcctccttgc cggtgcgctg
      241 atccaccagc cggatgcgca cgccgttgtt gaggaacgag agttcgcgca gccgcttgct
      301 gagcacttcg tagtggaagt cgaccacgcc gaagatcgcg tcatcgggca ggaagtgcac
      361 ctcggtgccg cgcttgtcgg tggcgcccgc gatcttcatc ggcgaggcct cgactggccc
      421 ttcctgggaa ttcagggtct cgatgacacg gtcctgcacc tcaccctggc ggaactcgag
      481 cgcgtgcacc ttgccgtcac gacggatggt caggcgcagc cacttgctga gcgcattgac
      541 gcacgacaca ccgacgccgt gcaggccacc cgagaccttg tagctgttct ggttgaactt
      601 gccgccggca tgcaactcgg tcagtgcaat ttctgcagcc gagcgcttgg gttcgtgctt
      661 gtcgtcgaac ttgataccgg tcggaatgcc gcggccattg tcgacgactg aaatggagtt
      721 gtcggtgtgg atggtgacga cgatgtcgtc gcagtagccg gccagcgcct cgtcgacagc
      781 gttgtcgacg acttcgaaca ccatgtgatg cagcccggtg ccgtcctgcg tgtcaccgat
      841 gtacatgccc ggacgtttgc gcaccgcctc cagtccttca aggatctgga tgctgcttgc
      901 gccgtagtcg cccttgccgt cggcttcgcc tccaggaatg ggctgattca tcggtggctg
      961 attcggtact gctgacatga attcctcgcg ttctcggcgt acgcatcggc tgcgccgatg
     1021 cacgcgcact tcgaacgatt agttagtgca tggggcttgg ggcggcccgg cgcctcatgc
     1081 agttcaacaa caaaatggcg cgtacaccga gtctggctaa attctcatcg gcatgacgac
     1141 gtacttgaac ttctcggaat ccggcagcgt catcagcgcg ctgccgagcg cgtcacccag
     1201 cgcgaacttc agctgttcgc tcttcacgtt gagcaacaca tcgagcagat agttgacgtt
     1261 gaatccgata tcgagcccgt cgccggcgta gtcgatctcg agttcttcct gggcgtcttc
     1321 ctggtcggcg ttggtcgagt tgatcttcag ggcgccggcc gccagcaaca gtcgcacgcc
     1381 cttgaacttg tcggtcgtca ggatcgcggc gcgctgcagc gcgtgcatca ggtcctcgcg
     1441 gccaatgaca aaggtcttcg tgttggccgc cggaacgact cgctggtaat cggggaactt
     1501 gccttcgacc agcttggaaa tgagttcgat gtcaccgaag ctgaacttga cctgcgtggc
     1561 cgccatctgc acgcgtaccg gatcctcgtt atcgggcagc aagcgtgaga gttcgaggat
     1621 ggtcttgctt ggaatgatgg cctcgatctt cgggctggcg ccgtccttct gcacttcaca
     1681 gagcgcaagc cggtgcccgt cggtcgcgac cgcgcgcacg gtggcaccgt cgaccacgag
     1741 cagcatgccg ttcaggtagt agcggatgtc ctgctgtgcc atcgcgaagt gaaccatcga
     1801 cagcaggtac ttgagcgttg ctgccggcag cgaaaagtcg gccgtgaact cggcctgcgc
     1861 gacggtcggg aactcctcgg ctgcgagcgt ctgcaggttg aaccggctct tgccgctggc
     1921 gaccgccagc tttttgcttg ccagcgagag ctgaacatcc gattccggca gtgcgcgcag
     1981 gatgtcgacg agcttgcgcg cggcgactgt cgtcgcggcg acgtccttgc ccgcaccgat
     2041 cggcgccgag gtctggatct gcagctcgag atccgttgcc gtgaaagaca cgcgctcgcc
     2101 gtccttgcgc acgaggatgt ttgcaaggat cggcagcgtc tggcgacgct cgacgattcc
     2161 gctcacaacc tgcaagggtt tgagcaacgc atcacgactg gctttgacga gttgcatttc
     2221 tcttttcttt ctctctagta gttagttaac aactaagcat tgcctgcacc ggggacaacc
     2281 cgcgcaatcg ccttcatatc agtcacctgc gcggccggaa gcactgtgga caagcgatct
     2341 gcctgcctgt gggccgcatg tggacgaacg ggacaacttt cctgccacgc gcttcgaggc
     2401 tcgaactgtc cacagctgtc cacaggcaca gcgagtttgt ccacaggaat tcatcagccc
     2461 ttcaaagtct gctcaagcac gtgcagtgcg tgattgagct cgccggcctt gacacgctcg
     2521 ccgccgatct tgcgcacggc gtgcagcacc gtcgtgtggt cgcgcccacc gaagagttcg
     2581 ccgatctcgg gcaggctctt ctgtgtcagt tccttcgcca ggtacatcgc gacctggcgc
     2641 ggcagcgcga tgttgctggg gcgccgcttg ctgtacatgt cggcgacctt aatcttgtag
     2701 taatcggcaa ccgtcttctg gatcagttcg accgtaacct gcccggtcga ggccgacagt
     2761 agatccttga gtgcctcgcg tgccacttcc acggtcacga cgcggccgtg gaacgacgag
     2821 aacgcgatga ccttgcgcag cgcaccttcg agctcgcgca cattgctgcg caggttcttg
     2881 gcaatgaaga aggcaacgtc ttccttcatg tcgacgcctt cgctctccgc tttcttcagc
     2941 aggattgcga cccgcatctc gagttccggc ggctcgatcg caaccgtcag gcccgaagcg
     3001 aaacgggaga ccaggcggtc ttccatgtcc ttcagttcct tcgggtaggt gtcgcaggtg
     3061 atgatgatct gtttgcggcc gacgaccagc gcttcgaatg cataaaagaa ctcttcctgc
     3121 gtgcgcgcct tgcctgcgaa gaactggata tcgtcgatca gcagcaggtc gagcgagtgg
     3181 tagtagcgct tgaagtcgtc gaaggccttg cgctggtagg cgcgcacgac atcgctgacg
     3241 tactgctcgg catggatgta gcgcaccttg gcatcgggct tgcgcgcaag gatgccgttg
     3301 ccgaccgcat gtatcaggtg cgtcttgccc aggcccacgc cgccataaag aaacagcggg
     3361 ttgtaggaac cgcctggatt ctccgcgacc tgcatcgccg cggcgcgggc gagctggttc
     3421 gccttgccgg tgacgaaggt atcgaaggtg aggttcgcgt tgagtcgtgc gcgctctaag
     3481 tgttccggac tgggcggcgc aacttccgcg agcgtgggga cctcgacctc ggaagcgaga
     3541 gcggccggaa aacgcagtgt ggctacggca gcgccgtttt ccaccgcgaa gaccaccgcc
     3601 accggacgct ggacgatgcg tgacgccgcg tacgcgatct gatctgaaaa ttgcgcgcgg
     3661 atcgcatcga gcttgagccg attcggtgca ccgatcgtca gtgtcgatgt ggattcgtcg
     3721 aagtcgatgg ccttgagtgg cttgatccag gcggaatact gttgcggcgt cagctcgctt
     3781 tcgagcagac gcgagcagtc gtgccagatg tgttgcattg agtgggtgtt cccgcttttg
     3841 ttgtcattgc gggagtgcct gcggcccgat agctgaaacc ggcccgcttt gcccccgccc
     3901 cgtcgtcaga acccgcagtc ctcggctgcg tgtgccctga cgaaaccgcg cattttactg
     3961 caagcgaggg ggttatccac agggcgtgac tggggcgaga caccggtagg gccgtgcaag
     4021 ggccgcgaat tagcgaggcg atctaaggta gtagatgctc acacctcatg caacgaaccg
     4081 actgcgcgtt gaagatttcc atattgcact gcaccatgga gttatccaca ggaaatccgc
     4141 cgacttattc aacattcgtc cggaccccgc ccaacgaccg catgaccacg aggtgcgcct
     4201 cgtttgacag tccattgcgg catcgcgtct aatagcgggc tcttcgctgt ggcgggaaaa
     4261 gcagcgaaaa atccgtacct tgcgtgactc gacgccggtg gttcgacttc ggtgaagcaa
     4321 cgccgcggcc agtcagcccg gcggcgttaa gccggggcga ctatttcgcg gcgcagcgag
     4381 tgccggcgta ccggctgcat cgcccgacgc gtccgccgaa caggaaagaa catgtctacc
     4441 aaacgtactt accagccttc caaagtcaag cgcgcccgca cgcatggatt ccttgtccgc
     4501 atgagatcgc gcggcggtcg tgccgtcatc gcggcgcgtc gtgcgaaggg tcgcaagcga
     4561 ctggcggtct gacggcatcg ggcacggcgc gtgtcgtgca tgccgatgac actgatccca
     4621 cacggaatgc cggagcgggc aaggctcgcc ccgacgacgc tcgcacgctc gggcgcgaac
     4681 gtcgcctgcg taggccgcgg gagtttgccg ccgtgcttgc cgcagatcgt gctcactcga
     4741 cgcgggctgc agggaagtgg ctctcgatga cggcagcctg ggacccgatc tcgcccggct
     4801 gcgtacggct cggcatcacc gtcagaaagc gcatggcgcg gcgggccatc gaccgtgcac
     4861 tggtcaagcg catcgcgcgc gaagcgtttc gccacgcagt ttcgggaatc gagcgcgtcg
     4921 ccgccggttc gggtgtgggc gtggacatca gcgtacggct caagcgtccc gtcgcgctgc
     4981 cggatgatcc gcagcggccc gcgttaaccc
//
LOCUS       DATIRT010000188         2949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1894499, whole genome shotgun sequence.
ACCESSION   DATIRT010000188 DATIRT010000000
VERSION     DATIRT010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1894499"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="VLN25_04465"
     CDS             <1..771
                     /locus_tag="VLN25_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HSQ09856.1"
                     /translation="SGHKCPFTTSWISWTQLESFSAWHNTLFATMNNGFGGMGARLVF
                     DSPLHQRHIENLANMSKQGLFIYKGRNNTADASFPSGECAMMTGSSGLYSSIKRNAKF
                     ASGISTLPYYPDVTGAPQNTVIGGASLWVMSGKKPIEYKGVANFFNYLSRPDVQAASH
                     QRTGYLPITMAAYKLTEESGFYKKNPGTDVAVTQMIRKTTDKSRGIRLGNFVQIRTIV
                     DEETEQVWSGKKTPKEALASSVKRGNEQLERFEKANKN"
     gene            859..1743
                     /gene="ugpA"
                     /locus_tag="VLN25_04470"
     CDS             859..1743
                     /gene="ugpA"
                     /locus_tag="VLN25_04470"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HSQ09857.1"
                     /translation="MEKRVRFRSLWLPWLLLAPQLAIISIFFFWPAAQALLQSVQMQD
                     AFGTTTEFVGMENFKRLFNDELYLDSFRITAIFSVLVAGCGLSLALLLAVFADRVIRA
                     ATVYKTLMVWPYAVAPAVAGVLWLFMFAPSIGMVSFWLRAAGFDWNHLLNANDAMALI
                     VMAAVWKQISYNFLFFLAGLQSIPKSLIEAAAIDGAGPWRRFWTIVFPLLSPTTFFLL
                     VINIVYAFFETFAIVDAATQGGPGRATTILVYKVYYDGFKGMDLGSSAAQSVVLMVIV
                     ITLTVVQFRYVEKKVQYS"
     gene            1807..2658
                     /gene="ugpE"
                     /locus_tag="VLN25_04475"
     CDS             1807..2658
                     /gene="ugpE"
                     /locus_tag="VLN25_04475"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HSQ09858.1"
                     /translation="MVERRPALSVLTHFVLLVGVLIVAFPVYVTFVASTQTAEAIVQA
                     PMSLVPGDQFFKNYASVLTGSDKGAGSSAPVGRMMWVSLVSALGIAFGKIVISLLSAF
                     AIVYFRFPFRNFFFWAIFVTLMLPVEVRIAPTYQVVSDLGMLNSYAGLTVPLIASATA
                     TFLFRQFFLTVPDELAEAARMDGAGPMRFFWDVLLPLSRTSIAALFVIQFIYGWNQYL
                     WPLLVTTQEDMYPVVMGIKRMIAGGDAVNEWNLVMATAMLAMLPPALVVLLMQKWFVK
                     GLVETEK"
     gene            2671..>2949
                     /locus_tag="VLN25_04480"
     CDS             2671..>2949
                     /locus_tag="VLN25_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSQ09859.1"
                     /translation="MASIEFSDVRKSYGSNQVVQGITLTVNNGEFVVLVGPSGCGKST
                     LLRMVAGLEEISGGTIRIGERIVNDLEPKDRDCAMVFQNYALYPHMSVY"
BASE COUNT          540 a          904 c          905 g          600 t
ORIGIN      
        1 tcgggtcaca aatgcccgtt cacaaccagc tggatcagct ggacgcagct cgagagcttc
       61 tccgcgtggc acaacacgct gttcgcgacg atgaacaacg gcttcggtgg catgggcgcg
      121 cgcctggtct tcgattcgcc gctgcaccag cgtcacatcg agaaccttgc caacatgtcc
      181 aagcagggac tgttcattta caaaggccgc aacaacacgg ccgacgccag cttcccctcg
      241 ggcgagtgcg cgatgatgac cggctcctca gggctttaca gcagcatcaa acgcaacgcc
      301 aagttcgcct ccggtatctc gacgctgccc tactatcccg acgttacggg cgcgccgcaa
      361 aatacggtga tcggtggcgc gagcctgtgg gtcatgtcgg gcaagaaacc catcgagtac
      421 aagggcgtcg ccaacttctt caattacctg tcgcgtcccg acgtccaggc cgcgagccac
      481 cagcgcaccg gctacctgcc aatcacgatg gcggcgtaca agctgaccga ggagtcgggc
      541 ttctacaaga agaatccagg caccgatgtc gcggtaacgc agatgatccg gaagaccacc
      601 gacaagtcac gcggcatccg cctcggcaat ttcgtccaga tcaggacgat cgtcgacgaa
      661 gaaacagagc aggtctggtc gggcaagaag actccgaagg aagctctcgc ctcgtcggtc
      721 aagcgcggca acgagcagct cgagcgtttc gaaaaggcca acaagaactg acgctcgcaa
      781 ttcgcaacgt gtgattggtc ggggccgcga gcaacgcagc acgcggcctg atcgcaggcc
      841 gactcatcac tgaacacgat ggaaaagcga gtccgcttcc gctcgttgtg gctgccctgg
      901 ctgctgctgg cgccgcagct tgccatcatc tcgatcttct tcttctggcc ggcggcacag
      961 gcactgctgc agtcggtaca gatgcaggat gcgttcggca ccacgaccga gttcgtcggg
     1021 atggagaact tcaagcggct tttcaacgac gagctgtatc tcgactcgtt caggatcacc
     1081 gcgatcttct ccgtactcgt cgcgggctgc gggctcagcc tcgcgctgct gctggcggtg
     1141 ttcgccgacc gggtgatccg cgctgcgacg gtctacaaga cgctgatggt gtggccgtat
     1201 gccgtggcgc cggcagttgc cggcgtgctg tggctcttca tgttcgcgcc gtcgatcggc
     1261 atggtctcgt tctggctgcg cgcagcgggc ttcgactgga accacctgct caatgccaac
     1321 gacgcgatgg cgctgatcgt catggcggcg gtgtggaagc agatcagcta caacttcctg
     1381 ttcttcctcg ccgggctgca gtcgatcccg aagtcgctga tcgaggccgc tgccatcgat
     1441 ggcgccggac cgtggcgccg cttctggacc atcgtctttc cgttgctttc gccaacgaca
     1501 tttttcctat tggtgatcaa catcgtctat gcgttcttcg agaccttcgc gattgtcgac
     1561 gccgcaaccc agggtggacc cggtcgggcc acgacgattc ttgtttacaa ggtgtactac
     1621 gacggcttca agggcatgga cctcggcagc tcggccgcac agtccgtcgt gctgatggtg
     1681 atcgtcatca ccttgacggt cgtgcagttc cgctacgtcg agaaaaaggt gcagtactca
     1741 tgagacgcga acgcgggtgg ccggcgaaca ccgccacggt ctggcaaacg gcaggaaggg
     1801 gttgagatgg tcgaacgccg cccggcgctt tcggtactga cccacttcgt gctgctcgtc
     1861 ggcgtgctca tcgtcgcgtt cccggtctat gtcactttcg tggcgtcgac ccagaccgcc
     1921 gaggcgatcg tgcaggcgcc gatgtcgctg gtccccggcg accagttttt caagaactac
     1981 gcctcggtgc tgaccggcag cgacaagggc gccggctcgt ccgcgccggt gggccggatg
     2041 atgtgggtaa gcctggtcag cgcgctggga atcgcattcg gcaagatcgt gatctcgctg
     2101 ctgtcggcgt ttgcgatcgt ctacttccgt tttcccttcc gcaatttctt cttctgggca
     2161 atcttcgtca cgctgatgct gccggtcgaa gtgcgtatcg cgcccaccta ccaggtcgtg
     2221 tccgacctgg gcatgctgaa cagttacgcg ggactgacgg tgcccctcat tgcgtcggcc
     2281 accgcgacgt tccttttccg ccagttcttc ctgaccgtgc cggacgaact ggccgaggcc
     2341 gcccgcatgg acggcgccgg accgatgcgc ttcttctggg acgtgctgct gccgctgtcg
     2401 cgcacctcga tcgcggccct gttcgtcatc cagttcatct atggctggaa ccagtacctg
     2461 tggccgctgc tcgtgacaac gcaagaggac atgtacccgg tagtcatggg catcaagcgg
     2521 atgatcgccg gcggcgatgc cgtcaacgaa tggaacctcg tgatggccac ggcaatgttg
     2581 gcgatgctgc cgccggctct ggtcgtgctg ctgatgcaga agtggttcgt caagggtctg
     2641 gtcgagacgg aaaagtgaga taggttctac atggcatcaa tcgagttttc ggacgtacgc
     2701 aagagttacg gcagcaatca ggtcgtgcaa gggatcaccc tgaccgtcaa caacggtgag
     2761 ttcgtcgtac tggtgggccc ctcaggctgc ggcaagtcga cgctgctaag gatggtggcc
     2821 gggctggagg aaatttccgg cggcacgatc cggatcggcg agcgcatcgt caatgatctc
     2881 gagccgaagg atcgcgactg cgcgatggtg tttcagaact acgcgctgta tccgcacatg
     2941 agtgtctac
//
LOCUS       DATIRT010000189         7230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1698961, whole genome shotgun sequence.
ACCESSION   DATIRT010000189 DATIRT010000000
VERSION     DATIRT010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7230
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1698961"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1506
                     /locus_tag="VLN25_04485"
     CDS             <1..1506
                     /locus_tag="VLN25_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HSQ09860.1"
                     /translation="NTRDSAGEGCLALTEALLADGRISVWERVRALVEQNQLSTAKRV
                     GQRALDAQGKPVDQKLLAQAIDRPAAFLVAHERRLTQIQRELALVAIVRLARDSPSEA
                     VEHANALNLHLTPEQRGIVWGRIGHMAALRLDPEANDWYRRGGEHVGVGPDAVRIDEV
                     LEWQVRAALRAGDWPTVKSTIDRMPPALRGDPAWIYWYGRALKTEGRPAEAQDHFAQI
                     APQFNFYGKLAAEELGIPIVLPPRALAPTAEDITPMRANPGFARAQKFYELGMRVEGH
                     REWNWQLRGMSDRQLLAAAEYARSVSLLDRMINTSDRTRSEFDFTQRFPSPFRDTLVQ
                     YAAPLGLDETWVYGLIRQESRFIMDARSHVGASGLMQLMPATARYVARKINVSNFSPA
                     RVNELDMNLMLGTAYLKLVLDDLDGSPILATAAYNAGPGRPRAWRSSLVRPVEGAIFA
                     ETIPFNETRDYVKKVMSNSVYYAALFENKAQSLKQRLGTIAPKSAGTTELP"
     gene            1592..2557
                     /locus_tag="VLN25_04490"
     CDS             1592..2557
                     /locus_tag="VLN25_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HSQ09861.1"
                     /translation="MQHTDILVIGGAGFVGRHIVSRLIDRGLKVTVPVRRRARAGHLI
                     TLPTIDVVEADIHDDATLAGLIAGRDAVINLVGILQGSRGSPYGPEFARAHVELPRRI
                     VKGCEAQRVRRYLHMSALGADAQGPSMYQRSKGDGEAAVRASSLDWTIFQPSVVFGPE
                     DKFLNTFAMLASVAPVLPIANAGARFQPIFVGDVADAFDHALDNAATYRKVYELAGPR
                     IYTLRELVQFATRAAGHPRPVIGLPAGLAKAQAIMMELAPGEPMMSRDNLDSMKRDNV
                     AARQPYVPPPELGIELRPMESEAAPYLAGQNLRTRLGSFRARARR"
     gene            2601..3248
                     /locus_tag="VLN25_04495"
                     /pseudo
     CDS             2601..3248
                     /locus_tag="VLN25_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011401704.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
     gene            3248..4417
                     /locus_tag="VLN25_04500"
     CDS             3248..4417
                     /locus_tag="VLN25_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09862.1"
                     /translation="MKFYRVGGCVRDALLGEEQQRRGDLDAALETDRDWVVVGGSPEE
                     MTKLGYRPVGRDFPVFLHPETHEEYALARTERKTAPGYKGFVFHAAPDITLESDLERR
                     DLTINAMALDEDGRLIDPHNGARDLRSGILRHVSPAFVEDPVRILRLARFAARFPDFR
                     IAPETAELVREMVVNGEADALVPERVMQEISRGLMERRPSRMLNVLVASGLIDRLYSE
                     LEDLGPTSAALDRAAAKGLVLPARFAVLASACRSPRTVADLMGKLRVPQESAQLARLL
                     VELREPLATAESSATVVDALERADAFRRPERFEVLLQTFETACEKSAERFRQALRAAA
                     EVDAGALASLHRNDPAEIPRAVRQARVVAVSGALHAGESPGESPSEQARDQHQRN"
     gene            complement(4382..4573)
                     /locus_tag="VLN25_04505"
     CDS             complement(4382..4573)
                     /locus_tag="VLN25_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HSQ09863.1"
                     /translation="MKWLLTTFLALLVLTALQPWLQRLGIGRLPGDVRFKINGKEVLL
                     PFTSTVLLSVALMLIARLF"
     gene            complement(4644..5720)
                     /locus_tag="VLN25_04510"
     CDS             complement(4644..5720)
                     /locus_tag="VLN25_04510"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HSQ09864.1"
                     /translation="MIASEIEARGGWIPFDRYMQRVLYAPGLGYYAAGARKFGDSASG
                     GDFVTAPEISPLFGQALAAQVAQVFEHTPACIVEFGAGSGRLAAELLQALRHRGLPCQ
                     RYAIVEVSADLRERQQALLAGEPVEWLTAAPADFAGVMLANEVLDVMPVRLFVKRGGA
                     VAERGVGTLDGGLRFVERTAPPDLVAAVGEIEREVGELPDGYGSEIGFDARAWMNSVA
                     QWLTRGALFTIDYGFPRREYYHPQRLMGTIMCHYRHFAHSDVFWLPGLNDLTAHVDFS
                     AMAQAAHESGLQVLGYTSQAHFLINCGLVDRLGSEHSPARSSEAHRLLSEAEMGELFK
                     VMAVGRELDAPLLGFARGDRTARL"
     gene            5858..6661
                     /locus_tag="VLN25_04515"
     CDS             5858..6661
                     /locus_tag="VLN25_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSQ09865.1"
                     /translation="MMTRRRSKSAGAALVTGAARRIGREIALALARDGWDIAVHYATS
                     RDEALDTVREIEALGRRAIAVNRDLAVEAGVRSLLAECAHELGEISCVVNNASLFEHD
                     SAATFTAEALVRCMRTNVAAPVLLAESLHATLSPGERGVVINLLDQKLWNPNPDFLSY
                     TLSKAALREATVLLAQALAPRIRVVGIAPGITLPSGDQSEAGFANAHAATPLGRSSRP
                     EDIASAVVFLARADGITGSTLLVDGGQHLVPSPRDVMFLTEPASPPAAA"
     gene            6658..7056
                     /locus_tag="VLN25_04520"
     CDS             6658..7056
                     /locus_tag="VLN25_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HSQ09866.1"
                     /translation="MMNGLLDPRLADCRRLFLRNYVVDANIGIHAFEKAGAQRLALNV
                     DLYIPLALSTPRHDRIHEVIDYDFIRLTIRHRLEQGHINLQETLVDDIARSLLAHPAV
                     RAVRVASEKPDVYADVDAVGVEVLQFKEGA"
BASE COUNT         1241 a         2456 c         2367 g         1166 t
ORIGIN      
        1 aacacgcgcg actcagcggg cgaaggctgc ctcgcactga ccgaggcatt gcttgccgac
       61 gggcgcattt cggtgtggga gcgcgtccgt gccctggtcg agcagaatca gctttccacg
      121 gccaagcgcg tcgggcagcg agcgctcgac gctcaaggca agccggtcga ccagaaactc
      181 ctggcccagg ccatcgatcg tccggccgcg ttcctggtcg cacatgagcg tcgcctgacg
      241 cagatccagc gcgagctcgc cctcgtggcg atcgtgcgcc tcgcccgcga cagcccgagc
      301 gaggcggtcg aacatgcgaa cgcgctcaac ctgcatctga cgccggagca gcgcggcatc
      361 gtctggggcc gcattggtca catggcggcc ctgcggctcg acccggaagc caacgactgg
      421 tatcggcgcg gcggcgagca tgtcggcgta gggccggatg cggtccgtat cgatgaagtg
      481 ctcgagtggc aggttcgcgc ggcgttgcgc gccggcgact ggcccaccgt gaagtcgacc
      541 atcgaccgca tgccgcccgc gctgcgtggc gacccggcct ggatttactg gtacggacgt
      601 gcattgaaga cagaaggccg gcccgccgag gcccaggatc actttgcgca gatcgcgcct
      661 cagttcaact tctacggcaa gctcgctgcc gaagaactcg gcatcccgat cgtgctgccg
      721 ccgcgcgcgc tcgcccccac tgccgaggac atcacgccga tgcgggccaa tccaggtttc
      781 gcacgcgcac agaagttcta cgaactgggg atgcgcgtcg aaggacaccg cgaatggaac
      841 tggcaattgc gcggcatgag cgaccggcaa cttctggccg cagccgagta cgcgcgatcg
      901 gtttcgctgc tcgaccgcat gatcaacacg tcggaccgca cgcggtccga gttcgacttc
      961 acacagcgct ttccttcgcc gttccgcgac acgctggtcc agtacgcggc cccgctcggg
     1021 ctggacgaaa catgggtgta cgggctgata cggcaggagt cccgcttcat catggacgcg
     1081 cgttcgcacg tgggtgcctc cggactgatg cagctgatgc cggcgacggc ccgttacgtg
     1141 gcgcgcaaga taaatgtcag caatttctca cccgcgcggg tgaacgaact cgacatgaac
     1201 ctgatgctcg gcacggccta cctcaagctc gtgctggacg atctcgacgg aagtccgata
     1261 ctcgcgacag cggcctacaa cgccggaccg ggacgcccgc gcgcctggcg ttcgtcattg
     1321 gtgcgaccgg tcgaaggtgc gatctttgcc gagacgattc cattcaacga gacgcgcgac
     1381 tatgtcaaga aggtcatgtc gaacagcgtc tactacgcgg ccctgttcga gaacaaagcg
     1441 cagtcgctga agcagcgcct cggtaccatc gcaccgaagt cggccggcac caccgagttg
     1501 ccttgaccgg gtcgcgtgcc ggccgcctgg cgcgagaccc gaccgccgca gaatagaatc
     1561 tcttttcgca ggcaccctcc tccggtcccc cgtgcaacat accgacatcc tggtcattgg
     1621 cggagccggc ttcgtcggcc gccatatcgt cagccggctc attgaccgcg ggctcaaggt
     1681 cacggtgccg gtgcgccgac gggcgcgggc aggccatctg atcacactgc cgacgatcga
     1741 cgtggtcgag gccgacatcc atgacgacgc gacgcttgcc gggctgatcg ctggacgcga
     1801 cgcggtgatc aacctggtcg gcatcctgca gggttcgcgc ggatcgccct acggccccga
     1861 gttcgcccgc gcgcatgtcg agctgccgcg ccgcatcgtc aaagggtgtg aagcgcagcg
     1921 cgtgcgtcgc tatctccaca tgagcgcgct cggggcggat gcgcaggggc cgtcgatgta
     1981 tcagcgcagc aaaggcgacg gcgaagccgc cgtgcgcgcc tcgtcgctcg actggaccat
     2041 cttccagccc tcggtcgtgt ttggaccgga ggacaagttc ctcaacacgt ttgccatgct
     2101 cgcgtcggtt gcgccggtcc ttccgattgc caatgccggc gcccgcttcc agcccatttt
     2161 cgtcggcgac gtggctgacg cgttcgacca tgcgctcgac aatgcggcca cgtaccgcaa
     2221 ggtctacgaa ctggccgggc cgcgcatcta cacactgcgc gaactcgtgc agttcgctac
     2281 acgcgcggca ggacatccgc ggcccgtgat cggcctgccg gccggtctcg ccaaggcgca
     2341 ggccatcatg atggagctcg cgccggggga accgatgatg tcgcgagaca acctggattc
     2401 catgaagcgc gacaacgttg cagcccggca gccctatgta ccgccaccgg aactgggcat
     2461 cgaactgcgg ccgatggaat ccgaggcggc gccgtatctc gccggccaga acctgcgaac
     2521 gcggctgggc agctttcggg cgcgcgcgcg ccgttgaaga tgccgatggc gcaacgcgac
     2581 gacgttcagg gcaatccgcg atgagccgcg tgctggtcat cggcaacaag aactactcca
     2641 gctggtccct gcgcgcctgg ttggcgctga aagcgtccgg agtgcaattc gaggaaagtc
     2701 gcattgcact cgaccaaccg cagaccaagg ccgagatcct gcggcattcg cccagcggca
     2761 aggtgccgtg cctggtcgac ggatctctcg tcgtatggga ctccctggcg atctgtgaga
     2821 cggcgaacga gcgctatgcc gcgggatcgc tgtggccgac cgatccggaa gcgcgtgcgc
     2881 acgcgcgtgc cgtcacggcc gaaatgcatt cggggttcgt cgcactccgt acccacatgc
     2941 cgatggacat acgctcgaac aaacctgacg tcggtgtgac ggcccagggc cacagcgacg
     3001 tcgccgcgga catccagcgc attcgtgcga tctgggccga ctgcctggcc cgcagcggcg
     3061 gaccgctgct ctatggcgaa ttctcgatcg ccgatgcgtt ctatgcgccg gtggtcacgc
     3121 gcttccggac ctaaggcata gccctgcaac cgccgctggg cgcgtacgcc gacgccgtat
     3181 tcgcgctgcc ggcgatgcag cagtgggtcg ccgcggctcg ggccgaaacc gaagtccttc
     3241 agtactgatg aagttctatc gcgtcggcgg ctgtgtgcgc gacgcgttgc tcggcgagga
     3301 acagcagcgt cgcggcgacc tagacgcagc gctcgaaacc gaccgcgact gggtcgtcgt
     3361 cggcggctcg cccgaggaga tgacaaagct gggctaccgg ccggtcggtc gcgactttcc
     3421 cgtctttctg catccggaga cgcacgagga gtacgcgctg gcgcggaccg agcgcaagac
     3481 agcgccgggc tacaaagggt tcgtttttca cgctgcgccc gacatcacgc tggaatcgga
     3541 tctcgaaagg cgcgacctga cgatcaacgc gatggcgctc gacgaggacg ggcgcctgat
     3601 cgatccgcac aacggggccc gcgatctgcg ttccggcatc ctgcgccatg tcagcccggc
     3661 gttcgtcgag gacccggtgc gcatcctgcg gctcgcgcgc tttgcggccc gctttcccga
     3721 ttttcggatt gcaccggaaa ccgccgaact ggtgcgcgag atggtcgtca atggtgaggc
     3781 cgacgccttg gtgcccgagc gcgtgatgca ggagatctca cgcgggctga tggaacgcag
     3841 gccttcgcgc atgctcaacg tgctggtcgc gagcggactc atcgatcgtc tctacagcga
     3901 gctggaagac ctgggcccga ccagtgccgc gctcgaccgg gcggcagcga aagggctcgt
     3961 gctgccggcc cgctttgccg tgctcgcgag tgcatgccgc agcccgcgaa cggtggccga
     4021 cctgatgggc aagctgcgcg tgccacagga gtcggcgcag ctggcacggc tgctggtcga
     4081 attgcgcgaa cccctggcga cggcagagtc ttccgccact gtcgtcgacg cgctcgagcg
     4141 cgcggatgcg tttcgccggc cggaacgctt cgaggtgctg ctgcagacct tcgagacggc
     4201 ctgtgaaaag tcggccgagc ggttccgtca ggcgctgcgc gctgcggccg aggtggacgc
     4261 gggcgcactc gcgagtctgc accgcaacga tccggccgag atcccgcgcg cggtgcgaca
     4321 ggcccgcgtc gttgcggtca gcggcgccct gcacgcgggt gagagtccgg gcgagagtcc
     4381 gtcagaacag gcgcgcgatc agcatcaacg caactgaaag caacacggtg ctggtgaacg
     4441 gcagcagcac ttcctttccg ttgatcttga agcggacgtc acccggtagc cggccgatgc
     4501 cgagccgctg cagccagggc tgcaatgcgg tcagcacgag cagcgcgagg aaggtagtga
     4561 ggagccattt catcgtgacg caacgagtgt acgcggcgca tggctccgtt ccaggagcgg
     4621 ccgagagagc cgccgtcacg ggctcacaga cgcgccgtcc gatcgccacg cgcgaagccg
     4681 agcaacggag catccagttc acggccgacc gccatcacct tgaacagttc gcccatttcc
     4741 gcctcggaca gaaggcgatg cgcttcgctg ctgcgcgccg gcgagtgctc cgacccgaga
     4801 cggtcgacca ggccgcagtt gatcaggaag tgcgcctgtg aggtgtatcc gagaacctgc
     4861 agtccgcttt catgggccgc ctgcgccatc gcactgaagt cgacgtgcgc cgtcaggtcg
     4921 ttcaggcccg gcagccagaa gacgtcgctg tgggcgaagt gccggtaatg acacatgatc
     4981 gtgcccatca gccgctgcgg gtggtagtac tcacgtcgtg gaaagccgta gtcgatcgtg
     5041 aacaacgccc cgcgcgtcaa ccactgcgcc accgagttca tccatgcgcg tgcgtcgaag
     5101 ccgatctccg aaccgtagcc gtcgggcagt tcgcccacct cgcgttcgat ctcgcccacc
     5161 gcggcgacta gatcgggcgg cgcggtgcgc tcgacgaacc ggagaccgcc gtcgagggtt
     5221 ccgacaccgc gctcggcgac cgctccgccg cgtttaacga agagccgcac cggcatcaca
     5281 tcgagcactt cgttggccag catgacgcca gcgaaatcgg ctggcgccgc cgtcaaccat
     5341 tcgaccggct cccccgcaag cagcgcctgc tgccgctcgc gcaggtcagc cgagacttcg
     5401 acgattgcgt atcgctggca cggtaggcca cggtgtcgca gtgcctgcag cagttctgcc
     5461 gcgaggcggc ccgatccggc gccgaactcg acgatgcagg ccggtgtgtg ctcgaacacc
     5521 tgggccacct gagccgcgag cgcctggcca aagagcggcg agatttccgg ggccgtgacg
     5581 aagtctccgc cgctcgccga atcgccgaat ttgcgcgcgc cggccgcgta gtaacccagt
     5641 cccggcgcgt acagcacccg ttgcatgtag cggtcgaacg gaatccagcc accccgggct
     5701 tcgatctccg acgcgatcaa cgcgcacagc ttttcactgt gcgcgcactg atcggccgag
     5761 gcttgtggca gcggggagct cgagcggtgc atcgagcggc atggtagcca agccccgcag
     5821 gacacgccgg ctacacttgt gcggttccgg aattgccatg atgactcgcc gcaggtcgaa
     5881 atctgccggc gctgcgctcg tcacgggcgc ggcgcgccgt attggtcgcg agatcgcgct
     5941 cgcgctcgcc cgcgacggct gggacatcgc ggtgcactac gccacgtcgc gcgacgaggc
     6001 gctcgatacc gtgcgtgaga ttgaagcact cggacgacgg gccatcgcgg tcaatcgcga
     6061 cctcgccgtc gaggccggag tacgcagcct gctggccgaa tgcgcacacg aactcggcga
     6121 gatcagctgc gtcgtcaaca acgcatcgct gttcgagcac gacagcgcgg ccacctttac
     6181 cgccgaggca ctggtccgct gcatgcggac gaacgttgcg gcgccggtcc tgctcgctga
     6241 gtctcttcac gcgacgctgt caccgggtga gcgtggcgta gtgatcaacc tgctcgacca
     6301 gaaattgtgg aatccgaatc cggacttcct ctcgtacacg ctgtccaagg ccgccttgcg
     6361 cgaggccaca gtgctgctcg cccaggcact cgctcctcgc attcgagtgg tcggcatcgc
     6421 gccgggcatc acgctgccct cgggtgatca gtccgaagcc ggctttgcca acgcgcacgc
     6481 cgcaaccccg ctcgggcgtt cgagccggcc cgaggacatt gccagcgccg tcgtgtttct
     6541 tgccagggcc gacggcatta ccggcagtac gctgctggtc gacggcggac agcatctcgt
     6601 ccccagtccc cgcgacgtca tgtttctcac cgagcccgca tcgcccccgg cggccgcatg
     6661 atgaacggac tgctcgatcc gcgcctggcc gactgtcgtc gcctgttcct gcgcaactat
     6721 gtggtggacg ccaacatcgg catccatgcg ttcgagaagg ctggggcgca gcgcctcgcg
     6781 ctcaatgtcg acctgtacat ccctctggcg ctgtcaacgc cgcggcatga ccggatccat
     6841 gaagtcatcg actacgactt catacggctg acgatccgcc atcgcctcga acagggtcac
     6901 atcaatctgc aggagaccct ggtcgacgac atcgcccgca gcctgctcgc acacccggcc
     6961 gtgcgcgccg tacgcgtggc gagcgaaaaa ccggacgttt atgccgacgt cgatgcggtc
     7021 ggcgtcgaag tgctgcagtt caaggagggt gcatgagtcc aacggcgggg tcccacggcg
     7081 agctgccgcg cgcgctgcag gacgacagca cgctcgtcgc cgcgcccgcg ggcgacccga
     7141 ccgatccgcg ccgcgcggcc aggctcgcgc atgagaatca caagctcgaa aagcgcctgc
     7201 tgcgcctcgc cgggcaggcg atcggcgact
//
LOCUS       DATIRT010000190         3239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1502028, whole genome shotgun sequence.
ACCESSION   DATIRT010000190 DATIRT010000000
VERSION     DATIRT010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502028"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..865)
                     /locus_tag="VLN25_04525"
     CDS             complement(200..865)
                     /locus_tag="VLN25_04525"
                     /inference="COORDINATES: protein motif:HMM:NF023044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSQ09867.1"
                     /translation="MSAAQSRLKQASAAGRSPEARNRARGFTLLELLVAITVLSVVSL
                     ISWRGLDTLVSTRERLEPEADDVRALLTAFGQMERDLAQVTNPKFLGLTASPVNVSIA
                     DGTTTLQLARVAPASADRPTEVQTVYYRVVEGTLIRQATAALPAFELTPTDRLESARL
                     LGQVRLMQVRTWSPNGGWVDPNIPQDTSGAPPGANAVVPGVEISIERENGRIFRRVLL
                     VGA"
     gene            complement(862..1236)
                     /gene="gspI"
                     /locus_tag="VLN25_04530"
     CDS             complement(862..1236)
                     /gene="gspI"
                     /locus_tag="VLN25_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HSQ09868.1"
                     /translation="MTPSRGFTLLEVLVALVIIGVALAASMRGAMSLTTTAEYTREKM
                     LAVMTAENRLLELRLARERLEQGENSVPCEQGGLTFQCVQAVKPTPNPFFRRVEVRVF
                     KTAPDGSPRMMAELMTVLPTNQ"
     gene            complement(1233..1766)
                     /locus_tag="VLN25_04535"
     CDS             complement(1233..1766)
                     /locus_tag="VLN25_04535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSQ09869.1"
                     /translation="MKALSARRTARPGGRLPCGGFTLIEMLVVIVLAGILISVVSISV
                     TPDPRQKLMREAQRIGQLMTLAADEARIRQLPIVWEADLRGYRFVSEVGGERQLLTGD
                     DLLRERTWETPLTRLSVQDGAGPQPAQVLLGPGAPPVRMAIAREWIQPRWRLELSNED
                     STVRLDFDESGRATVVQ"
     gene            complement(1824..2267)
                     /gene="gspG"
                     /locus_tag="VLN25_04540"
     CDS             complement(1824..2267)
                     /gene="gspG"
                     /locus_tag="VLN25_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HSQ09870.1"
                     /translation="MKVLRSPRAGGFTLIEIMVVITILGILAALIVPRVVGRTDDARI
                     AAARQDIASVMQALKLYRLDNGRYPTTEQGLQALITKPSTEPIPNNWKQGGYLERSSV
                     PRDPWGQPYQYLNPGLRGEIDIFSFGADNQSGGQNTDADIGSWSL"
     gene            2497..3078
                     /locus_tag="VLN25_04545"
     CDS             2497..3078
                     /locus_tag="VLN25_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09871.1"
                     /translation="MLKKFAAAMVHLLFFAGVVAIGAYWSVRIFTPAPTSAPPPLPPP
                     PLRDPDATAAARMFGKVEVAQVAVASNIQSLGAFAAGKNSSAILVVDSKPPRVFLVGQ
                     EVVPGTRLVSIESEVIVLDSPSGRQEVRLPPRPAVAISGGAPMQTFTRDGNTLTAPSA
                     SGAPAIAPRPSNAPPQPPPAMPQNTPGSPPQSQ"
BASE COUNT          535 a         1075 c         1057 g          572 t
ORIGIN      
        1 acgatcaacg tggcgagcgt ggcgacgaac aaggcaagca acacggctgc gccgcgttgc
       61 ttgcctgtcg gcagacgtga tggccccacc ccagcccgcc cccgcggggc gggagacaca
      121 gcggcgccgc gctgcctgcc actctgcgga cgtgatgccc ccactctcgc aagccccttg
      181 tgtgttgcag cggttgctct catgcaccca ccagcagcac gcggcgaaag atccggccgt
      241 tctcgcgctc gatcgagatt tcgacgccgg ggacgactgc gttagctcct gggggcgcgc
      301 cgctggtgtc ctgtgggatg ttcggatcga cccacccgcc gttcggtgac caggtgcgta
      361 cctgcatcag gcgcacctgg ccgagcaatc gggccgactc caggcggtcg gtgggcgtca
      421 gttcgaacgc cggaagagcc gcggtcgcct gacggatgag tgtgccttcg acgacgcggt
      481 agtacacggt ctgaacttcg gtcgggcggt cagccgatgc gggtgccacc cgcgccagct
      541 gcagcgtcgt cgtgccgtcg gcgatcgaca cgttgaccgg tgacgccgtc aggccgagga
      601 acttgggatt ggtcacctgc gcaagatcgc gttccatctg tccgaatgcc gtcagcagtg
      661 cgcgtacgtc gtcggcctcc ggctctagcc gctctcgcgt gctcaccaat gtgtcgagcc
      721 cgcgccagga gatcagcgac acgaccgaca gcacggtgat cgcgaccagc aattcgagca
      781 gcgtgaatcc gcgcgcgcgg ttacgagcct ccgggctgcg ccctgcggcg ctagcttgct
      841 tgagacggct ctgcgctgcg ctcattggtt ggtcggcaac acggtcatca gctcggccat
      901 catgcgcggg gaaccatcgg gcgcggtctt gaacacccga acctcgacac gccggaagaa
      961 cgggtttgga gtcggtttca ccgcctgtac gcactggaag gtcaggccgc cctgctcgca
     1021 cggcacgctg ttttcgccct gttcgagccg ctcgcgcgcc aggcgtagtt cgagcaggcg
     1081 gttctccgcc gtcatgacgg ccagcatctt ttcgcgtgtg tactcggccg tggtcgtcag
     1141 cgacatcgcg ccccgcatcg acgcggcgag cgccacgccg atgatcacca gcgcaaccag
     1201 cacttcgagg agggtgaacc cgcggctagg cgtcattgaa ccaccgtggc gcggccggat
     1261 tcgtcgaaat cgaggcggac cgtcgagtcc tcgttggaca gttcgagacg ccagcgcggc
     1321 tgaatccact cgcgcgcaat cgccatgcgc accggcggtg caccggggcc gagcagcact
     1381 tgcgccggtt gcggccccgc gccgtcctgg accgacagcc gcgtcagcgg tgtttcccag
     1441 gtgcgttcgc gcagcaagtc atcgccggtc agcagttgcc gttccccgcc cacctcggac
     1501 acgaatcgat agccgcgcag atctgcctcc cagacaatcg gcaactgccg gatccgggcc
     1561 tcgtcggccg cgagcgtcat cagctggccg atgcgttgcg cctcgcgcat gagcttctgc
     1621 cttggatcgg gcgtcacgct gatcgacacg acagagatca ggatgccggc gagcacgatg
     1681 acgaccagca tttcgatcag ggtgaagccg ccacaaggca agcgtccccc tggacgtgcg
     1741 gtgcggcgtg ccgacagtgc cttcatgggg tctggatatc cgatcgtctg gcaaggcagg
     1801 gcggtacgat gaccgcgccg cgcctacagc gaccaggaac cgatgtccgc gtcggtgttc
     1861 tgtccgccag actggttgtc ggccccgaag ctgaagatat cgatctcacc tcgcagcccc
     1921 gggttcaggt actggtaggg ctggccccac gggtcgcgcg gcaccgaact gcgctcgaga
     1981 taaccgcctt gcttccagtt gttcggaatc ggttcggtgc tcggcttggt gatcagcgcc
     2041 tgcaggccct gctccgtggt cgggtagcgc ccgttgtcga gccggtagag cttcagtgcc
     2101 tgcattaccg aggcgatgtc ctggcgtgcg gcggcgatgc gggcatcgtc agttcgcccc
     2161 acgactcggg ggacgatgag cgccgccagg attccgagga tcgtgatcac gaccatgatc
     2221 tcgatcagcg tgaaaccgcc ggcgcgcggg gaacggagga ccttcatcgt actcatatga
     2281 tcactgccca aagcgaaaga aagcttgaag ttacgcgccg aaggcattga acttcttgtg
     2341 ttttgggcgc ggacaatggg gactgcgacg gatattatca acgcaagtgt ctgacccgac
     2401 ccctcttaga cgggattcgt cgtggcgact aatgaaagag gatgagcgtt atccgcgcgg
     2461 cctgagcgat catcggcgcc cctgcctgga cttgcgatgc tgaaaaagtt tgctgcagcc
     2521 atggttcacc tgctcttttt cgcgggtgtg gtggccatcg gcgcgtactg gtcggtgcgc
     2581 atcttcacac cggcgccgac ctcggcgccg ccgcccctgc cgcccccgcc cttgcgcgat
     2641 cccgatgcga ccgccgcggc ccgtatgttc ggcaaggtcg aggtggcgca ggtcgcggtc
     2701 gcgtccaaca ttcagtcgct gggcgctttt gctgccggca agaattccag cgcaatcctc
     2761 gtcgtagaca gcaagccgcc gcgggtgttc ctggtcgggc aggaagtagt tcccggcact
     2821 cggctggtgt cgatcgagtc ggaggtgatc gtcctagaca gtccgagcgg gcggcaggaa
     2881 gtccggctcc cgcctcggcc cgccgtggcc ataagcggtg gcgcgccgat gcagaccttc
     2941 acacgagacg gcaatacact gaccgccccc agtgcgagcg gggccccggc catcgcgccg
     3001 cgtccgtcca acgcaccccc gcagcccccg ccggccatgc cgcagaatac tcctgggtcg
     3061 ccaccgcagt cgcagtaggg tgcgccgcgg ttgcgccgcg tatagctggc gcgtcgcgga
     3121 cgtttcagac gtaaccaaaa gtaagtcgcg tcggccggcg cgcgtgctca ccgttaaatg
     3181 ggctcaccct cacaggagat gctcatgaaa actcggatac tcatcgctgc tgtactggc
//
LOCUS       DATIRT010000191         4490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1600281, whole genome shotgun sequence.
ACCESSION   DATIRT010000191 DATIRT010000000
VERSION     DATIRT010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1600281"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VLN25_04550"
     CDS             <1..184
                     /locus_tag="VLN25_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HSQ09872.1"
                     /translation="VEKDASGRPMRTINHMLKLFLIDRNGVVREIYALDYLHPAMMIN
                     DIETLLMEDRAPVVGR"
     gene            361..603
                     /locus_tag="VLN25_04555"
     CDS             361..603
                     /locus_tag="VLN25_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09873.1"
                     /translation="MKRILSAAAALLFSTAALAFHCPADMKKIDEAMAKNPKLTEAQM
                     AEVKKYRTEGETLHKAGKHQESVDTLAKAMKILNIS"
     gene            866..3412
                     /locus_tag="VLN25_04560"
     CDS             866..3412
                     /locus_tag="VLN25_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HSQ09874.1"
                     /translation="MSRGAQLALPSAEGWFAAQGWQPFPFQREVWQCIAEGRSGLLHA
                     TTGSGKTYAVWFGLLNRALAGELHGGGLRLLWLTPMRALAADIARALEAPLLQLGLDW
                     SVGVRTGDTGSVERARQSQRLPEALVTTPESLTLLLARADSPLLLEGVELVVVDEWHE
                     LMGSKRGVQLQLALARLRRFARERAERSRSAPRPRERQGDGGPEGGEGSTPQPELAVW
                     GLSATLGNTGHAMEVLLAGAPGVMVRGAVPKEIVVDTLLPDEPGRFPWGGHLGIQMLE
                     PVVREIESASTTLVFTNTRSQAEIWYQSLLEARPKWAGVIALHHGSLDKEVREWVECG
                     LKEGRLKAVVATSSLDLGVDFLPVERVLQIGSPKGVARLLQRAGRSGHAPGRASRVTI
                     VPTHTLELVEAAAARAAVAQGQVEPRFAPERPLDVLVQHLVTLAVGTGFREDEALAEI
                     RTAWSYRELSDEEFAWVLDFVGRGGESLRAYPEYHRVVRDGEGVYRVQDRRIARRHRM
                     SVGTIVSDASMLVKMLNGRNLGTIEESFITRMRKGDSFVFGGRLLELVRTHEMTAYVQ
                     LAKRRSGTVPRWSGAKMPLSTEMADASLRELEWAMAGRYDSPEMRLARPLIELQAKWS
                     AVPTRAALVVESLRSREGRHLFVYPFAGRHAHLGLASLLAYRIGQGAPSTFSIAINDY
                     GFELLSPVDIDWMGAWQRRSATLLGESDLLDDVLASLNAGELALRRFREVARIAGLVF
                     QGFPGAPKSMKQLQASSGLFYEVFRKFDAGNRLLAQAEREVLEQELDIGRLRTALARM
                     RGQRIVYRLLARPSPFAFPLMVERLREQVTTEKLSARIERMLAELEQAAR"
     gene            3671..4066
                     /locus_tag="VLN25_04565"
     CDS             3671..4066
                     /locus_tag="VLN25_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HSQ09875.1"
                     /translation="MATKKAKKATKKTAKKAAPKKAAKKTAKKAVKKVAKKAPAKKAA
                     KPKVKRKPNAAFMRPLMPSGPLAAIVGSVPMPRTEVTSKIWAYIKKNKLQDSVNRRMI
                     NADDKLKALFGKAQASMFELTKYVSKHLK"
     gene            4203..>4490
                     /locus_tag="VLN25_04570"
     CDS             4203..>4490
                     /locus_tag="VLN25_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HSQ09876.1"
                     /translation="MPSIVLATLNARYAHASLGLRCLRANMGELRDDTVIREFVIRTP
                     PEQIVADLIAQQPRIVGLGVYIWNVRQTTRVVELLKAAAPHVVVVLGGPEVS"
BASE COUNT          806 a         1425 c         1500 g          759 t
ORIGIN      
        1 tgtcgagaaa gacgcgagcg gccggccgat gcgcacgatc aatcacatgc tgaagctctt
       61 cctgatcgac cgtaatggcg tcgtgcgcga gatctacgcg ctcgactacc tgcacccggc
      121 gatgatgatc aacgacatcg agacgctgct gatggaggat cgcgctcccg tggtcgggcg
      181 ttgaggccat tgcgggctat gccgggaagt acagcacccg ccaagacagt gacaccacgc
      241 cgcgagcggg gtcttccgcc ggcaccggtt ccggggatgt ggccgcgcgt tcctggagtt
      301 agactgcaga tcgctttgct ttcacgcaag gcctcttttt cctctcaagg agagtcacct
      361 atgaaacgca tcctgagcgc cgcagcggcg ctgctgttct ccaccgctgc actggccttc
      421 cactgccccg ctgacatgaa gaagatcgat gaggccatgg ccaagaatcc gaaactgacc
      481 gaagcgcaga tggccgaagt caagaagtac cgcaccgagg gtgaaacgct gcacaaggcc
      541 ggcaagcacc aggagtcggt cgacacgctg gccaaggcga tgaagattct caacatctcg
      601 taattgttct ctgccgcacg acgccgcctg cgggcggcgt ttcttttttc ggatcacgcc
      661 acaggtaacg taccgatcga ctgcccggct gtgatgcgca atttgcagat tcgcttcgct
      721 ggacagtgcc gtgattgagg aacggcgcgg cctgccgcgc ggttcgaatg tcggcactca
      781 gctgcgcgcg cggcgcgcga agcaggtgcg ggaacagcgg gcgcgcgagg ccgaagcagc
      841 cacccgcgcc gctgcggcaa cgcccgtgtc tcgaggcgcg caactcgcac tgcccagtgc
      901 cgaaggctgg ttcgcggccc agggctggca accgttcccc ttccagcggg aagtctggca
      961 gtgcatcgcc gagggccgct cgggcctgct gcacgcgacc accggcagcg gcaagaccta
     1021 cgcggtctgg ttcgggctgc ttaaccgggc cctcgcgggc gaactacacg gtggcggcct
     1081 gcgcctgctg tggctgacgc cgatgcgtgc actcgcggcc gacatcgcgc gtgcactcga
     1141 agcgccgctg ctgcagctcg gtctcgactg gagcgtcggg gtgcgcacgg gtgataccgg
     1201 ctcggtcgag cgcgcccgac agtcacaacg attgcccgag gcgctcgtca caacgcccga
     1261 gagcctgacc ctgctgctgg cgcgcgccga ttcgccgttg ttgctcgaag gcgtcgaact
     1321 cgtcgtggtc gacgagtggc acgagctgat gggcagcaag cgcggcgtac agctgcagct
     1381 cgcgctcgcg cgcctgcgcc gcttcgcgcg cgagcgcgcg gagcgcagca ggtctgcgcc
     1441 ccgccctcgg gagcggcagg gggatggggg gcctgaagga ggcgaaggct ctacaccgca
     1501 gcctgagctt gcggtgtggg gactgtctgc gacgctgggc aataccgggc acgcgatgga
     1561 agtgttgctt gcgggtgcgc cgggcgtgat ggttcgcggc gcggtcccca aagagatcgt
     1621 cgtcgacacg ctgctcccgg acgagcccgg acgctttccc tggggcggtc atctcggcat
     1681 tcagatgctc gaaccggtcg tacgcgagat cgaaagcgcc tcgaccaccc tcgtcttcac
     1741 aaacacacgc tcgcaggccg agatctggta ccagtcgctg ctcgaggcgc ggcccaagtg
     1801 ggcaggcgtg atcgcgctgc atcacggttc gctcgacaag gaagtgcgag agtgggtcga
     1861 atgcgggctc aaggaggggc gattgaaggc ggtggtcgcc acctccagcc tcgacctcgg
     1921 ggttgacttc ctgccggtag aacgcgtgct gcagatcggg agcccgaagg gcgtggcgcg
     1981 attgctgcaa cgtgccggcc gcagcggaca cgcgccaggg cgggcgtcgc gcgtgacgat
     2041 cgtgccgacc catacgctcg aactcgtcga agccgcggcc gcgcgcgcag ccgtcgcgca
     2101 aggccaggtt gaaccgcgct tcgctcccga gcgaccgctt gacgtgctgg tgcagcatct
     2161 cgtgaccctg gccgtcggca cgggctttcg cgaggacgag gctctggcgg agatccgcac
     2221 ggcctggagc taccgcgagc tcagcgacga ggaatttgcg tgggtgctcg acttcgtcgg
     2281 ccgcggcggc gagtcactgc gtgcctatcc cgaataccac cgcgtcgtcc gcgatggcga
     2341 aggcgtctat cgggtgcagg accgtcggat cgcgcggcgg caccgcatgt cggtggggac
     2401 catcgtctcc gacgcctcga tgctggtgaa aatgctcaac ggccgcaacc tcggcaccat
     2461 cgaggagtcg ttcatcacgc ggatgcgcaa gggcgattcc ttcgtcttcg gcgggcgcct
     2521 gctcgaactg gtgcgcacac atgagatgac ggcgtacgtg cagctggcca aacggcgcag
     2581 cggtacggtg ccacgatggt ccggagccaa gatgccattg tcgaccgaga tggccgatgc
     2641 gtccctgcgc gagctggaat gggccatggc cggacgctac gactcacccg agatgcggct
     2701 ggcgcggccg ctgatcgaac tgcaggccaa gtggtcggcc gtgccgacgc gtgcggccct
     2761 ggtcgtcgaa tcactgaggt cgcgcgaggg tcggcacctg ttcgtctacc ccttcgccgg
     2821 acgccatgcg cacttgggcc tcgcgtcgct cctggcctac cggatcgggc agggcgcgcc
     2881 ttcgaccttc tcgatcgcga tcaacgacta cgggttcgag ctgctctcgc cggtcgacat
     2941 cgactggatg ggcgcgtggc aacgccgctc ggcgacactg ctcggcgagt cggacctgct
     3001 cgacgacgtc ctggcgagcc tgaacgccgg cgagctcgcg ctgcggcggt tccgcgaagt
     3061 ggcgcgcatt gccggactgg ttttccaggg atttccgggc gcacctaagt cgatgaagca
     3121 actccaggca agctcgggac tgttctacga ggtcttccgc aagttcgacg ccggcaaccg
     3181 gctgctggcc caggccgagc gcgaggtgct cgagcaggag ctggacatcg ggcgcctgcg
     3241 cacagcgctc gcgcgcatgc ggggccagcg catcgtctac cgcctgctgg cgcggccatc
     3301 gccgtttgca tttccgctga tggtcgaacg cctgcgcgag caggtgacga cggaaaaact
     3361 gtccgcgcgt atcgagcgca tgctcgccga actcgagcag gcggcccgct agaacctaca
     3421 agatattgtg tgacgatggg ctgctggcca atatctagta caccagtcgt ggtgtctggc
     3481 acagcaagtc gaagtgtttt cagaggggaa acggcgttgt tcacacgcaa tttccctctg
     3541 aaaagttgat ttctcctctt gtaattcgtt gcgtttcctc taggatcgcg gtcacccaac
     3601 gagagcgctc tcttgcattt cactcgagaa cgctgtcgct gtggcactca aatcctcaga
     3661 ggagaactcg atggcaacaa agaaggcaaa gaaagcgacg aagaagacgg cgaagaaggc
     3721 tgcgccgaaa aaggctgcaa agaagacggc aaagaaggcc gtaaagaagg ttgcgaagaa
     3781 ggcaccggcc aagaaggccg ccaagccgaa ggtcaagcgc aagccgaacg ccgcgttcat
     3841 gcgtccgctg atgccgagtg gcccgctcgc cgcgatcgtc ggctcggtgc cgatgccgcg
     3901 taccgaagtc accagcaaga tctgggcata catcaagaag aacaagctgc aggactcggt
     3961 caatcgccgc atgatcaacg ccgacgacaa gctgaaggcg ctgttcggca aggcgcaggc
     4021 ttcaatgttc gaactgacca agtacgtcag caagcacctg aagtagtcgc gcgcgggctc
     4081 acggcccgcc accgacacaa tgatcgcccg gtgccatctc tggcccgggc gattttttct
     4141 tcgcgctctg attgtggcga tcgaacggcc cgtcgcggcg caacgctatg cttccttgag
     4201 ttatgccaag catcgtgctc gccacgctca atgcccgcta cgcgcatgcg tcgctcgggc
     4261 tgcgctgcct gcgggcgaac atgggggaat tgcgcgacga cacggtcatc cgggagttcg
     4321 tgatccgcac tccgcccgag cagatcgttg ccgatctgat tgcgcagcag ccgaggatcg
     4381 tcggcctggg cgtctacatc tggaacgtcc ggcagacgac ccgcgttgtc gagttgctga
     4441 aggccgcagc gccacacgtc gtcgtggtcc tcggcggacc cgaggtcagt
//
LOCUS       DATIRT010000192         1853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_617464, whole genome shotgun sequence.
ACCESSION   DATIRT010000192 DATIRT010000000
VERSION     DATIRT010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617464"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /gene="trxB"
                     /locus_tag="VLN25_04575"
     CDS             <1..985
                     /gene="trxB"
                     /locus_tag="VLN25_04575"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HSQ09877.1"
                     /translation="STKHARVLILGSGPAGYSAAVYAARANLKPVLITGLAQGGQLMT
                     TTDVDNWPADANGVQGPDLMARFLAHAERFNTEIIFDHIHTARLTEKPIRLIGDSAEY
                     TCDALIIATGASAMYLGLPSEQAFMGKGVSGCATCDGFFYKNQDVAVIGGGNTAVEEA
                     LYLSNIAKKVTLVHRRDKFRAEPILVDHLMEKVKEGKVELRVHSVLDEVLGNESGVTG
                     ARIRNAQTGAAQDLVLQGVFIAIGHRPNTEIFAGQLDMANGYIITKTGLAGMATATSV
                     PGVFAAGDVQDHVYRQAITSAGTGCMAALDAQRYLENLQPAPATTGETIAA"
     gene            1008..1658
                     /locus_tag="VLN25_04580"
     CDS             1008..1658
                     /locus_tag="VLN25_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HSQ09878.1"
                     /translation="MASRIKDLSQLAPLRESLNRAAREREAAAADQQRVARQQQTAAD
                     EFRAVVGAVTPLAIKQRVEHDRPPHPPIPQKRIEDDQQVLIASVSDEFEIDTLLHTDA
                     ELSFRRPGIGPDVLRKLRRGEWVIQDHLDLHGARVDEARELLAGFLREALRRGFRCVR
                     VVHGKGLGSKDKVPVLKGKARVWLAQREEVIAFCQARPAEGGSGALVVLLRPAQRP"
     gene            1655..>1853
                     /locus_tag="VLN25_04585"
     CDS             1655..>1853
                     /locus_tag="VLN25_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HSQ09879.1"
                     /translation="MIEFPTLLFVVEVVAVLACAFSGFAEAQRKDMDLVGVYVVAFIT
                     AFGGGPLRDVLLDRRPFFWVDH"
BASE COUNT          332 a          607 c          613 g          301 t
ORIGIN      
        1 atcgaccaaa cacgcacgcg tgctgatcct gggttcggga ccggcgggat actcggcggc
       61 ggtctacgcg gcgcgcgcca atctcaagcc ggtactgatc accggcctgg cccagggcgg
      121 acagttgatg acgacgaccg atgtcgacaa ctggcctgcc gacgccaacg gagtgcaggg
      181 cccggatctg atggcgcgct tccttgccca tgccgagcgt ttcaacacgg aaatcatttt
      241 cgaccacatc cacaccgcca ggctcactga aaagccgata cgcctgatcg gcgacagcgc
      301 cgagtacacc tgcgatgcgc tgatcatcgc caccggggcc agtgcgatgt acctcggatt
      361 gccgtccgaa caggcgttca tgggcaaggg cgtctcgggc tgcgcgacct gcgacggttt
      421 cttctacaag aaccaggacg tggcggtcat tggcggcggt aacacggcgg tcgaggaagc
      481 gctgtatctg tccaacatcg caaagaaggt cacgttggtc catcgtcgcg acaagttccg
      541 cgccgagccg atcctggtcg accacctgat ggaaaaagtg aaggaaggca aggtcgagct
      601 gagggtgcac agcgtgctcg acgaggtgct cggcaacgag tccggcgtca ccggcgcccg
      661 catcaggaac gcccagaccg gcgccgcgca ggatcttgtg cttcagggcg tgttcatcgc
      721 catcgggcat cggccgaaca cggagatctt cgccggccag ctggacatgg ccaacggtta
      781 catcatcacg aaaaccggac tcgcgggaat ggcaaccgcg accagcgtac ccggcgtatt
      841 tgctgccggc gacgtgcagg accatgtcta ccggcaggcg atcacgagcg ccggaaccgg
      901 ctgcatggcc gcactggacg cacagcgcta cctcgaaaac ctgcagccgg ctccggccac
      961 caccggggaa acgatcgcgg cctgaggcgt ccggatggtg acgatccatg gcctcgcgca
     1021 tcaaggactt gtcgcaactc gcgccgctgc gtgagtcact gaaccgtgcc gcgcgggagc
     1081 gcgaagccgc cgccgcggac cagcagcgcg tcgcccgcca gcagcagacg gccgcggacg
     1141 agtttcgtgc cgtcgtcggc gccgtgacgc cgctcgcgat caagcagcgc gtcgagcacg
     1201 accggccgcc gcatccgcca attccgcaaa aacgcattga agacgaccag caggtcctga
     1261 tcgcttcggt gtcggacgag ttcgagatcg acacgttgct gcacacggac gcggaactgt
     1321 cgttccgtcg tcctggcatc ggccccgatg tcctgcgcaa gctcaggcgc ggcgagtggg
     1381 tgatacagga ccatctcgac ctgcacggcg cgcgcgtcga cgaggcacgc gaactgctgg
     1441 cgggcttcct gcgcgaggcg ctccggcgcg ggtttcgttg tgttcgcgtc gtccatggca
     1501 agggcctggg atccaaggac aaggtcccgg tgctcaaggg caaggcacgc gtctggctcg
     1561 cgcagcgtga ggaggtcatc gccttctgcc aggcgcgccc ggcagaaggc ggcagcggcg
     1621 cgctggtggt gctgctccgt cccgcgcagc ggccgtgatt gaatttccga cgctgctgtt
     1681 cgtcgtcgaa gtggtcgcgg tcctggcctg cgcgttttca gggttcgccg aagcgcaacg
     1741 caaggacatg gacctggtcg gcgtctatgt cgtagccttc atcacggcct tcggcggtgg
     1801 cccgctgcgc gacgtactgc tcgatcgccg gcccttcttc tgggtcgacc acc
//
LOCUS       DATIRT010000193        12526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_322595, whole genome shotgun sequence.
ACCESSION   DATIRT010000193 DATIRT010000000
VERSION     DATIRT010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12526
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322595"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VLN25_04590"
     CDS             <1..661
                     /locus_tag="VLN25_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HSQ09880.1"
                     /translation="RLASRVLRINGRGIFAVNDRDHGDGRPLLVWIDDLLRSRGVRDA
                     DREIWLHTFPRVLGYVFNPVSFWFAHRADGALRAVVCEVNNTFGERHCYLLAHEDGRP
                     LQYGEAVESRKVFHVSPFCHVAGNYRFRFMLSSPASGAAPRWVGRIDYDDDDGPLLLT
                     SIAGTLVPLTDRALLRAFVAYPVFTFGVVARIHWQALRLWLKRVPFISKPEPPALHVT
                     R"
     gene            666..1913
                     /locus_tag="VLN25_04595"
     CDS             666..1913
                     /locus_tag="VLN25_04595"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HSQ09881.1"
                     /translation="MSNVAALDSRADLDTARFPSDARLALRLLDRMRQGRLDLTFPDG
                     QHAYYGDLRSGERADLRLANWNLVREALKSGDIGFAEAYIAGDWDSPDLVPAIEFFVA
                     NRDAADEFIYGSFLGRLAYRIRHLLNRNTRAQSRKNIHAHYDLGNDFYSLWLDRTMSY
                     SSALFEHTDRRAGPHEVASTDELEQGQYAKYARVLDELALPSGAHLLEIGCGWGGLAE
                     VAARAGLTVTGLTLSAEQLAYGRDRLARAGLHGDLQLRDYRDEAGEYDGIASIEMFEA
                     VGESFWPSYFATLKRCLKPGGRVCLQTIVIADRLFERYRAGTDFVQQYIFPGGMLPSP
                     SVFRARAHAAGFDVLNEHRFGMDYASTLATGRARFHARLDDVRALGFDDRFVRIWDFY
                     LAYCEAAFARENTDVVQYTLEVR"
     gene            1924..2493
                     /locus_tag="VLN25_04600"
     CDS             1924..2493
                     /locus_tag="VLN25_04600"
                     /inference="COORDINATES: protein motif:HMM:NF027363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HSQ09882.1"
                     /translation="MRADTRPVATLVLAALLAVAAHVPTSVASTLHDTPDHVRRHIDD
                     ARPYGSGRLTWFGFHVYDARMFVPRNFKPSNPSAQPFALEITYARSLEGRAIAERSRD
                     EIARLGLGSDAQRQRWLADMVALFPDVKSGQQLVGIYRPGSSTRFYVDGGFLGEVADP
                     EFGRAFFAIWLDPRTSAPQLRASLLSAPH"
     gene            2528..3832
                     /locus_tag="VLN25_04605"
     CDS             2528..3832
                     /locus_tag="VLN25_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009668093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSQ09883.1"
                     /translation="MTAATPMVGASGRVTATPVGRPTQRSLAAYGLLGAPLAMMALPV
                     YVLVPKFYFDATGLALSIIGAVLLITRLADAFIDPLLGAWVDRSKRHSIVRPVLVGMP
                     PLVLGFVLLFLPPAGLSAMAAAAWLAVTLMAAYFGYSVASIAYQAWGAGLADTDGDRA
                     HVTAWREGAGLVGVILASVVPYALGAPSLVALFLAALAIALTVLVRYAPRPPVRNPAE
                     SSSAPLTVALPLATSRFRWLLAVFVANGIAAAIPASLVLFFVADRLQLEPSSGLFLAL
                     YFIAGAASMPMWVRLAHRIPLEAVWLIGMVAAVAAFVWAHALGAGDFTAFALICALSG
                     VALGADLALPPALLARVIDRNGHTGLHEGAYFGLWNLANKLNLALAAGIALPTLGLLG
                     YVPGTRAPEALAALAFAYALLPCALKLVAAALLATAWRTRRL"
     gene            3841..4380
                     /locus_tag="VLN25_04610"
     CDS             3841..4380
                     /locus_tag="VLN25_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3833 domain-containing protein"
                     /protein_id="HSQ09884.1"
                     /translation="MIARALIAFAALLLAGCSAIDPKVYRDERPALELQRYFNGTLEG
                     HGLFQDRSGLVQRRLVVTIKASWAGDVGTLDEDFVWSDGKREKRIWTLRRDTDGATGT
                     RWIGTAADVIGEARGVVSGNALNWTYTFDLRTDDGKRYEVAFDDWMFLIDERVMLNRA
                     VMSFFGFRVGEVLISFRKL"
     gene            4380..5150
                     /locus_tag="VLN25_04615"
     CDS             4380..5150
                     /locus_tag="VLN25_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSQ09885.1"
                     /translation="MALNPKIRTWRGQRVWVIGASYGIGAALARRLINEGAHVAISAR
                     SRDLLVAIAERTDALVAVLDVTDPASVKKAAQQVIGAWGGIDLVMIVAGTHTAMSART
                     FDLPRATRLFEVNVMGVLHCLDAVLPQLLKQDRGGVAIVSSVAGYRGLPKALIYGPTK
                     AALINLAESLYLDLAPRGLGVYLVNPGFVDTPLTQKNDFRMPALIPADEAARLTLKGI
                     ADGEFETHFPKRFTRILRLLRTLPYPAYFAAVRRFTGT"
     gene            5167..5598
                     /locus_tag="VLN25_04620"
     CDS             5167..5598
                     /locus_tag="VLN25_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HSQ09886.1"
                     /translation="MTVDARVTRLQHFFETLSRSSLAAIGTVYADDAGFKDPFNEVRG
                     LPSVRRVFEHMFDQVTDPRFVVTRAVSQGDHAFLTWDFEFRMNRSPDQPQVIHGASHI
                     QFAPDGRVAYHRDYWDAAEELYEKLPILGGLMRFLKRRAAG"
     gene            5617..6489
                     /locus_tag="VLN25_04625"
     CDS             5617..6489
                     /locus_tag="VLN25_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010113645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HSQ09887.1"
                     /translation="MAFLYPELFKSLESVRWNMETDVPWKEFDGTRLSDDQARTIKMN
                     AITEWSALPATEMFLRDNLDDSDFSAFMSVWFFEEQKHALVLMEYLQRFRPELVPTEE
                     ELHAVRFQFDPAPRLETLMLHFCGEIRLNHWYRRAAEWHTEPVIKAIYRLLSQDEARH
                     GGAYLRYMKKALGEVGDAARAAFAKIGVLMASARRTDKAMHPTNLHVNQSLFPNDTVQ
                     SRLPDPGWLERWLDEQIRFDKEWETKVVDRILHNMSLLFERTFNSVQDLNRYRKEIAV
                     RLRTPPADPIVQPA"
     gene            6575..7573
                     /locus_tag="VLN25_04630"
     CDS             6575..7573
                     /locus_tag="VLN25_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018057787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HSQ09888.1"
                     /translation="MIPRHVPLVETTRGGTTECVHYGSIAVVDPAGKLISAVGDPDSL
                     NFTRSALKPLQALPFVEDGGLGRYGFTSHELAMMCASHSGESVHIGIVQRMLAAVGVD
                     DSALQCGCHAPGFFAATESSAPSGVKWSPLFHNCSGKHAGFLAYARMHGQPTENYLDN
                     GHPLQQRIRMIVARFAQGDGVAQGIDGCSAPNFALPLRRLAQTYARLAAQETPELAAL
                     YFAMTRHPDLVSGTRRTDLAIMQTGAGDWVSKAGADGMQAIGVASHGFGIAIRIADGS
                     PRALHAATVEVLQQLGVLEDSSGTPLAVYDCPPIRNYRGIETGGFVPVFKLRKQPS"
     gene            complement(7606..9708)
                     /locus_tag="VLN25_04635"
     CDS             complement(7606..9708)
                     /locus_tag="VLN25_04635"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HSQ09889.1"
                     /translation="MAQTQPDSTAPMPPVAKRIAHVVKSPHGDRVDEYYWMRDDDPTS
                     KRPEIIEHLNAENAYADAMLAHVNSLRDRLVTEMRARIKEDDSSVPAYDNGYWYWRRF
                     DAGAEYPILLRQRGGPSSPNGSAPEEVVLDIPRLARPHAYYNIGATAVSPDNRWLAYA
                     EDTVGRRIYTLRFRNLVTGELAAESITGVLADAEWAADSATVFYLRQDPQTLVRGTVF
                     RHRLGTDPAGDMLIYDEQDPELFTGLARSASRRFLMIQLEGYDTTETRVIPLAAPATA
                     PVVVLPRRKDVRTYADHLGDRWVLRTNEAAQNFRLVAAPDSAPDDRANWRDIVPARAD
                     AAVDAFALFERAIVIEERVEANSRIRVLPTDGRAPFTVVADEPAFSMNLAGNPDPAVL
                     VARYTYTSMTTPTTTYDVDLASGERRLRKVQPVIGYDATKYVTERIWAPARDGQRIPV
                     SLVYRRDRFKRDGSASLYIDGYGAYGLANDPYFSSNRVSLLDRGFVVALAHVRGGAEL
                     GQGWYEAGRLLAKQNTFNDFVDVTEHLVRERFGARDRVFASGGSAGGLLMGVIANQAG
                     DRYRGIALHVPFVDAVTTMLDESIPLTTNEWSQWGDPRRKSHYDYMLAYSPYDNIRAQ
                     EYPAMLVTTGLWDAMVQYYEPAKYVARLRATKTDRNPLLFHVTMEAGHSGKSGRFERL
                     AEAAREFAFFLDLAGVSD"
     gene            complement(9820..10482)
                     /locus_tag="VLN25_04640"
     CDS             complement(9820..10482)
                     /locus_tag="VLN25_04640"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSQ09890.1"
                     /translation="MQRSELDHLVVASTRLDDGIEWVGQHLVVRPQPGGQHAAMGTHN
                     ALLRLGARCYLEVIAIDPDGEPPVRPRWFALDDAMMQARLASGPALVTWALRCESLAG
                     ACARVPDLGDILSMSRGDFHWRIAVPEDGSLPWGGVLPAAIQWSTGDDESTPHPCDRL
                     PDSGCELLTLDLMHPAAVLGSAGIVSMFRELRVVGPIDLRPGPVRLAARIRTMAGDVT
                     LA"
     gene            complement(10815..11633)
                     /locus_tag="VLN25_04645"
     CDS             complement(10815..11633)
                     /locus_tag="VLN25_04645"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSQ09891.1"
                     /translation="MSTPDPYLLGYRQAEQERLEQQSLQLAHESEGLFEQIGVREGWR
                     VVEIGCGPRGCLGLLSKRVGASGRVVGVERSTEQVERARQFVSQSQLMNVEVLDADAR
                     ATALPEKTFDLATARLVLVNVPRPEEIVAEMIRLLRPGGVIALHEPDSTTQRCDPPLA
                     AQTRLLQILNSYAEMNGIDRSIGLRMPRMLREFGLLDIRVKPLVHVYPLGHARRMLVL
                     EFVENARTRILEKELIAVAELDELMAALRQHLENPSTLVVSSLFLQTWGRTPEQ"
     gene            complement(11630..12511)
                     /locus_tag="VLN25_04650"
     CDS             complement(11630..12511)
                     /locus_tag="VLN25_04650"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HSQ09892.1"
                     /translation="MVPLAPGDAADIAARTLGVEISRLLKTPVLTINRPGAGGAVGTN
                     SVVQARKDGYTILFAQNSALTFRTVLDPKSVAYDALRDLVPLGTASRTPTILVVQSDA
                     PYRTLPELIEYSRKNPGQVHIGHPGSGSVGDFCVQLINALTGTELVPIPYAGATPAII
                     ALRGRHIEGVVLSLGALSAHLKSGALRGLVASSRNPEFVDIPTMRELGYQEELFGIWF
                     SFLAPAGIPEDARTALVGAIEQAVNAPAIASRLASLGILQSYATPEQTAAEIRTEFKR
                     AGEIARKTGLITSGPSK"
BASE COUNT         2110 a         4094 c         4030 g         2292 t
ORIGIN      
        1 ccggctggca agccgtgttc ttcgaatcaa tggccgcggc atcttcgcag taaacgaccg
       61 cgaccacggt gacggtcggc ccctgctggt ctggatcgac gatctcctgc gaagccgtgg
      121 cgtcagggat gccgacagag agatctggct gcacacgttt ccgcgcgtgc tgggttacgt
      181 gttcaacccg gtcagtttct ggttcgcgca tcgtgccgac ggcgcgctgc gggccgtggt
      241 ctgtgaggtg aacaacactt ttggcgagcg tcactgctac ctgctggcgc acgaggacgg
      301 ccgtccgctg cagtacggag aagcagtgga atcgcgcaag gtgtttcatg tctcgccgtt
      361 ttgccacgtc gcggggaact accgcttccg tttcatgctc agttcgcccg cctcgggcgc
      421 agccccacgc tgggtcgggc gcatcgacta cgacgacgac gacggtcccc tgctgctgac
      481 gtcgatcgcc gggacgctgg tcccgctgac agaccgggcg ctgctgcgcg cgttcgtcgc
      541 gtacccggtc ttcacgtttg gcgtggtcgc acgaattcac tggcaggcgc tccggctctg
      601 gcttaagcgc gtccccttca tttccaagcc ggaacctccg gcgctgcatg tcacccgctg
      661 aactcatgag caacgtcgct gccctcgact cgcgcgcgga cctggacacc gcgcgctttc
      721 cttccgacgc ccgcctggcg ctgcgcctgc tcgaccgcat gcgccagggc cggctcgacc
      781 tgacctttcc cgatggccag cacgcctact acggcgacct gcgctccggc gagcgagccg
      841 acctgcgcct ggccaactgg aatctcgtca gggaggcgct gaaaagcggc gacatcggct
      901 tcgctgaggc ctacatcgcc ggcgactggg attcgcccga cctggtacct gcgatcgagt
      961 tcttcgtcgc caaccgtgac gctgcggatg aattcatcta tggctcattc ctcggccgac
     1021 tcgcctatcg catccgccac ctgctgaacc ggaacaccag ggcacagtcc aggaagaata
     1081 tccacgcgca ctacgacctc ggcaacgatt tctattcgct gtggctcgat cgcacgatgt
     1141 cgtactcgag cgcactcttc gagcacaccg atcggcgcgc cgggccgcac gaggttgcgt
     1201 caaccgacga actcgaacag ggccagtacg ccaagtacgc acgtgtactc gacgagctag
     1261 cgctgccatc cggcgcacac ctgctggaga tcggttgcgg ctggggtggg ctggccgagg
     1321 tcgccgcacg cgcggggctg acggtcaccg gattgacact gtcggccgaa caactggcgt
     1381 acggacgtga tcggctcgcg cgcgccggac tgcacggcga ccttcagttg cgggactacc
     1441 gtgacgaggc gggtgagtac gacggcatcg cgtcgatcga gatgttcgag gccgtcggcg
     1501 agtcattctg gccatcctac ttcgcgacgc tgaagcgctg cctgaagccc ggcgggcgcg
     1561 tctgtttgca gacgatcgtg atcgccgatc gcctgttcga gcgctatcgc gccggcacag
     1621 acttcgtcca gcagtacatc tttcccggcg ggatgctgcc gtcaccctcg gtgttccgtg
     1681 cgcgggccca cgccgccggg ttcgacgtcc tcaacgaaca tcgcttcggc atggactacg
     1741 cgagcacgct cgccaccggg cgggcgcgct ttcacgcgcg gctcgatgat gtgcgcgcac
     1801 tgggtttcga tgatcgcttc gtgcgcatct gggacttcta tctggcctat tgcgaggccg
     1861 cctttgcgcg ggagaacacc gatgttgtcc agtacacgct cgaagtgcgc tgaggtggcg
     1921 acgatgcgtg ccgacacgcg cccagtggct accttggtgc tggctgcact gctggcagtc
     1981 gcggcacacg taccgaccag cgtcgcttcg accctgcatg acacaccgga ccacgtacgc
     2041 cgtcatatcg acgatgcacg gccttacgga agcgggcgcc tgacctggtt cggtttccac
     2101 gtgtacgacg cacgcatgtt cgtcccgcgc aatttcaaac cgtccaaccc gtcggcgcag
     2161 ccgttcgcgc tcgagatcac ctacgcccgc agtctcgaag gacgtgcgat cgccgagcgc
     2221 agtcgcgacg agatcgcgcg cctcggtctc ggttcggacg cacagcgcca gcgctggctt
     2281 gccgacatgg tcgctctgtt tcccgacgtc aagtccggcc agcagctggt gggcatctac
     2341 cggcccggca gcagcacccg cttctatgtc gatggcggct tcctcggcga agtcgccgac
     2401 ccggagttcg gtcgggcgtt cttcgcgatc tggctggacc cgcgcacgag cgctccgcag
     2461 ttgcgcgcga gcctgctgtc ggcgccgcac tgatgtccgg accgaccaca ccacgccgag
     2521 cgtctcggtg accgccgcca cgcccatggt cggcgcatcg gggcgcgtca cggcgacgcc
     2581 cgtcggccgg ccgacgcagc gcagcctggc cgcgtacggc ctgctcggtg cgccgttggc
     2641 gatgatggcc ttgcctgtct acgtgctggt gccgaagttc tatttcgacg ccaccgggct
     2701 tgcgctgtcg atcatcggag ccgtgcttct gatcacgcgg ctggcggatg ccttcatcga
     2761 tccgttgctc ggagcgtggg tcgatcgttc gaagcgccac tccattgtcc ggcctgtcct
     2821 ggtcgggatg ccgccgctgg tgctgggctt cgttctcctc tttcttccgc ctgccgggct
     2881 atccgcgatg gcagccgcgg cgtggctggc cgtgaccctg atggcggcgt acttcggtta
     2941 ctcggttgcg tcgatcgcat accaggcctg gggcgccgga ctggcggata cggacggcga
     3001 tcgggcgcat gtcaccgcat ggcgcgaagg ggcgggcctg gtcggcgtaa tcctcgcgtc
     3061 ggtcgtgccg tacgcgttgg gtgccccgag tctggtcgca ctcttcctgg ccgcgctggc
     3121 catcgcgctc accgtgctgg tgcggtatgc gccgcgaccg ccagtgcgca accctgccga
     3181 gtcgtctagc gcaccactga cggtcgcgct gccgctagcc acgtcgcgct tccgctggtt
     3241 gctcgctgtg tttgtcgcca acgggatcgc cgccgcgata ccggcatcgc tggttctgtt
     3301 cttcgttgcc gaccggctgc aactcgagcc gagttcgggt ctgttcctgg cgctgtactt
     3361 catcgcgggt gccgcgagca tgccgatgtg ggtgcgcctc gcacaccgga tcccgctgga
     3421 agcggtgtgg ctaatcggca tggtggccgc ggtggcggcg ttcgtgtggg cccatgccct
     3481 gggcgccggt gatttcacgg catttgcact gatttgtgcg ttgtccgggg ttgcactggg
     3541 agccgacctg gcattgccgc cagccctgct ggcccgcgtg atcgatcgaa atggacacac
     3601 cgggctgcac gaaggcgcgt acttcggcct ttggaatctt gcgaacaaat tgaatctggc
     3661 actggccgcg ggcatcgcgc tgccgacgct gggcttgctc ggctacgtgc cgggcacacg
     3721 cgcaccggaa gcgctggccg cactggcctt tgcatatgcc ctgctgccgt gcgcgctgaa
     3781 gctcgtggct gccgcgctgc tcgctaccgc ctggcgcacc cgccgccttt aaggaatcac
     3841 atgatcgctc gcgcactcat tgccttcgcc gcacttctgc tcgccggctg ttccgccatc
     3901 gacccgaagg tctaccgcga cgaacgaccg gcgctcgagc tgcagcgcta cttcaacggg
     3961 acgctggaag gccatggcct gttccaggat cgcagcggcc tggtgcagcg ccgcttagtc
     4021 gtgacgatca aggcgagctg ggccggcgat gtcggcacgc tggacgaaga cttcgtctgg
     4081 tcggatggca agcgtgagaa gcgcatctgg acgttgcgcc gtgatacgga tggggcgacc
     4141 ggtacgcgct ggatcggcac cgcggccgac gtgatcggcg aagcgcgcgg cgtggtcagc
     4201 ggcaacgcac tgaactggac ctatacgttc gatctcagga ccgacgacgg caagcgctac
     4261 gaagttgcct tcgacgactg gatgttcctg atcgacgagc gcgtgatgct gaatcgcgcg
     4321 gtcatgagtt tcttcggttt ccgggtcggc gaggtgctga tctcgttccg caagctgtga
     4381 tggcgctcaa tccaaagatc cgaacctggc gcggccagcg cgtctgggtc atcggcgcgt
     4441 cctatggcat cggtgcggcg ctcgcgcggc ggctgatcaa cgagggcgcg cacgttgcga
     4501 tatcggcgcg cagccgcgac ctgctcgttg cgatcgccga acgcaccgac gcgctcgtcg
     4561 ccgtgctcga tgtcaccgat ccggcctcag tcaagaaggc ggcacagcag gtcatcgggg
     4621 cgtggggtgg catcgacctg gtgatgatcg tagcgggcac gcacaccgcg atgagcgcaa
     4681 ggacgttcga cctgccacgt gcaacccggc tgttcgaggt gaacgtcatg ggcgtgctgc
     4741 attgcctgga cgctgtgctg cctcaactgc tgaagcagga ccgcggtggc gtggcgatcg
     4801 tttcgtcggt ggccggttac cgcggactgc ccaaggctct tatctacggt ccgaccaaag
     4861 cagcgctgat aaatctggcg gagtcgctct acctcgacct ggcgccgcgc gggctgggtg
     4921 tgtatctggt caatccggga tttgtcgaca caccgctgac acagaagaac gacttcagga
     4981 tgccagccct gatccccgcc gacgaagccg cacgcctgac gctcaaaggg attgccgacg
     5041 gcgagttcga aacccatttc cccaagcgct tcacccgcat cctgcggctg ctgcgcacgc
     5101 tgccgtaccc ggcatacttc gctgccgttc gtcggttcac cgggacctag gtttcggcgc
     5161 cccacaatga cagtcgatgc ccgcgtcacg cgcctgcagc atttcttcga aaccctgtcg
     5221 cgcagttcgc tcgcggccat cgggaccgta tatgccgatg acgcggggtt caaggaccct
     5281 ttcaacgaag tgcgcggcct gccgtcggta cggcgggtct tcgagcacat gttcgaccaa
     5341 gtgactgacc cgcggttcgt cgtcactcgg gccgtatcgc agggcgatca cgcgttcctg
     5401 acatgggatt tcgagttcag aatgaatcga tcgcctgacc agcctcaggt aatccacggc
     5461 gccagtcata tacagttcgc tccagacggt cgcgtggcgt atcaccggga ctactgggac
     5521 gctgcggagg aactgtacga aaagctgcca atcctgggcg ggctgatgcg gttcctgaaa
     5581 cgtcgcgccg ccgggtgagt ccggctggag acaaacatgg catttctgta ccccgagctg
     5641 ttcaaatcgc tggaatccgt gcgctggaac atggagacgg atgtgccgtg gaaggagttt
     5701 gacggcacgc ggctgtccga cgaccaggcc cgcacgatca agatgaacgc catcaccgag
     5761 tggtcggcac tgccggccac cgagatgttc ctgcgcgaca acctcgacga ctctgacttc
     5821 tccgccttca tgtcggtctg gtttttcgag gaacagaagc acgcgctggt gctgatggag
     5881 tacctgcagc gcttccggcc agagctcgtg ccgaccgaag aggagttgca cgccgtgcgc
     5941 ttccagttcg acccggcgcc ccgcctggaa acgctgatgc tgcacttttg cggcgaaatc
     6001 cggctcaacc actggtaccg ccgcgctgcc gaatggcaca ccgagccggt gatcaaggcc
     6061 atctaccgtt tgctgtcgca ggacgaggcg cggcatggcg gtgcctatct gagatacatg
     6121 aagaaggcgc tcggcgaggt tggcgatgcg gcgcgtgcag cattcgccaa gatcggcgtg
     6181 ctgatggcct cggcacggcg caccgacaag gcaatgcacc cgaccaatct ccatgtaaat
     6241 cagtcgcttt ttccgaatga caccgtacaa tcgcgcctcc cggatccggg ctggctcgaa
     6301 cgctggctcg acgaacaaat acgattcgac aaggaatggg agaccaaggt cgtcgatcgg
     6361 atcctgcaca acatgtcgct gctgttcgag cggacgttca attcggtgca ggacctgaac
     6421 cgctaccgca aggaaattgc ggtgcggttg cgcacgcctc cggccgaccc gatcgtccag
     6481 cccgcctgag cactcgcgcc gcaatggcgc gcggggctgg catgcaatgc gccgtgcaac
     6541 cgcgctccgc gcgcggcgtt gccgaaggtt tccgttgatc ccgcgtcacg ttcccctggt
     6601 cgagaccacg cgcggcggaa cgaccgagtg cgtgcattac ggctcgatcg cggtggtcga
     6661 tcccgcgggc aagctgattt ccgcggtcgg cgatccggac tcgctcaatt tcacacgctc
     6721 ggccctgaag ccgctgcagg cactgccctt cgtcgaggat ggcggtctgg gtcgctacgg
     6781 attcacctcg cacgaactgg ccatgatgtg tgccagtcac agcggcgagt cggtgcatat
     6841 cggcatcgtg cagcgcatgc tcgctgccgt cggagtcgac gattccgcac tgcagtgtgg
     6901 ctgccacgcg cccggcttct tcgctgcgac tgaatcgtca gcgccctccg gggtcaagtg
     6961 gtcaccgctg ttccacaact gttcgggcaa gcatgccggc tttctggcct atgcgcgcat
     7021 gcacggtcag ccgaccgaaa actatctgga caacggacat ccgctgcagc agcggatccg
     7081 catgatcgtc gcgcgcttcg cccagggtga cggcgtcgcg cagggcattg atggctgcag
     7141 cgcgcccaat ttcgcactgc cattgcggcg cctcgcccag acctacgccc ggctggcggc
     7201 gcaggaaacg ccggagctgg cggcgctcta cttcgcaatg acgcgacatc cggatctggt
     7261 gtcgggcacg cgccgcaccg atctcgcgat catgcagacc ggcgcaggcg actgggtcag
     7321 caaggccggc gccgatggca tgcaggccat cggcgtggcc agccacgggt tcggcatcgc
     7381 catccgcatc gccgacggaa gcccgcgcgc gcttcacgcc gctacggtcg aggtgctgca
     7441 gcaactcggt gtgctggagg attcgagcgg cacgccgctc gcggtctacg actgtccgcc
     7501 catccgaaac taccgcggca tcgagaccgg cggcttcgtg ccggtgttca agttgcgcaa
     7561 gcagccctcg tagtcgcccg ttccttcgac cgttccgtcc gtcggctagt ccgacacccc
     7621 ggcgagatcg aggaagaacg cgaattcgcg cgccgcctcg gcaagccgct cgaagcgacc
     7681 cgacttgccg ctgtggccgg cttccatcgt gacgtgaaac agcaatggat tgcggtccgt
     7741 cttggtggca cgcagccggg cgacatattt ggccggctcg tagtactgca ccatcgcgtc
     7801 ccacagcccg gtcgtgacga gcatcgccgg atactcttgc gcgcgaatgt tgtcgtacgg
     7861 cgaataggcg agcatgtagt cgtagtgtga cttccggcgc gggtcgcccc actgcgacca
     7921 ttcgttggtc gtcagcggaa tcgattcgtc gagcatcgtc gtcacggcgt cgacgaaggg
     7981 cacgtgcagc gcaatgccgc ggtagcggtc gccggcctgg ttggcgatca cgcccatcaa
     8041 caggcccccg gcggagccgc ccgaggcgaa taccctgtcc cgcgcaccga agcgctcgcg
     8101 caccaggtgc tccgtcacgt cgacaaagtc gttgaaggtg ttctgtttgg caagcaagcg
     8161 gcccgcttcg taccagccct ggccgagttc ggcaccgcca cgcacgtggg caagcgcaac
     8221 gacgaagccg cggtcgagca gtgacacgcg gttgctgctg aagtacgggt cgttggccaa
     8281 gccgtaggcg ccgtagccat cgatgtacag cgacgcactg ccgtcgcgct tgaagcggtc
     8341 acggcggtag accagtgata ccggaatgcg ctgaccgtcg cgcgccggcg cccagattcg
     8401 ctcggtgacg tacttcgtgg cgtcgtatcc gatcacgggc tgcaccttgc gcaatcgtcg
     8461 ctcacctgag gcgagatcca cgtcgtaggt cgtggtcggg gtggtcatcg acgtgtacgt
     8521 gtagcgcgcg acaagcaccg cgggatcggg attgcccgcc aggttcatcg agaatgcagg
     8581 ctcgtcggcc acgaccgtga acggcgctcg tccgtccgtg ggcagcacgc ggattcggct
     8641 gttcgcttcg acacgctcct cgatgacgat cgcacgctcg aacagcgcga atgcgtcgac
     8701 cgccgcgtcg gcacgcgcgg gcacgatgtc gcgccagttt gcgcggtcgt cgggtgcgct
     8761 gtcgggcgcc gccaccagcc ggaagttctg cgcggcctcg ttggtacgca ggacccagcg
     8821 atcgcccaga tgatcggcat acgtgcgcac gtccttgcgc cgcggcagga cgacgaccgg
     8881 cgcggtcgcc ggcgccgcga gcgggatgac gcgtgtctcg gtcgtgtcgt agccttcgag
     8941 ctggatcatc aggaaacgcc gcgacgcgct gcgcgcgaga cccgtgaaga gctccgggtc
     9001 ctgttcgtcg tagatcagca tgtcaccggc cgggtcggtg ccgagccgat gccggaagac
     9061 agtcccgcgc accagcgtct ggggatcctg gcgcaggtag aacacggtgg cgctgtccgc
     9121 ggcccactca gcgtcagcca gaacacccgt gatcgactcc gccgcgagtt ctcccgtgac
     9181 gagattgcgg aagcgcaatg tgtagatgcg acggccgacg gtgtcttccg cgtaagcaag
     9241 ccaccggttg tccgggctga ccgcagtggc cccgatgttg tagtacgcgt gcggccgcgc
     9301 gagccgcgga atgtcgagca cgacctcctc gggagcgctc ccattcggcg acgacggccc
     9361 gccgcgctgc cgcagcagga tcggatactc ggcgccggca tcgaagcgcc gccagtacca
     9421 gtagccgttg tcgtaagccg gcaccgaact gtcatcctcc ttgatgcggg cgcgcatttc
     9481 cgtgacaagt cgatcccgca gcgaattcac atgcgccagc atcgcgtcgg cgtacgcgtt
     9541 ctcggcgttc aggtgctcaa tgatctccgg gcgcttggac gtcggatcat cgtcacgcat
     9601 ccagtaatac tcgtcgacgc gatcgccgtg cgggctcttg accacgtgcg cgatgcgctt
     9661 ggccaccggc ggcatcggcg cggtggagtc aggctgggtc tgcgccatgg cgaatcctgc
     9721 aaacaaaatg agacacgtgc aaagaagtgt tcgaacggca gccgacagcc cgcctcgcgt
     9781 gatgcgagcc cgatcgttca aggaattcat ggacgcagat catgccagcg tgacgtcacc
     9841 cgccatcgtc cggatgcgcg cggcgaggcg gaccggcccg ggtcgcaggt caatcggccc
     9901 gaccacccgc agctcgcgga acatcgaaac gatgccagcg gaaccgagca cagccgccgg
     9961 atgcatgaga tccagcgtga gcagctcaca gccactgtcg ggtaaccggt cgcacggatg
    10021 cggagtcgat tcgtcgtcgc cggtcgacca ctggatggcc gccggcagca caccccccca
    10081 cggcaggctt ccgtcctccg gcacagcgat gcgccaatga aagtcgccgc ggctcatcga
    10141 caaaatatcg ccgaggtccg gcacccgcgc gcacgcgccc gccagtgatt cacagcgcag
    10201 cgcccacgtg accagcgccg gacccgacgc gagacgggcc tgcatcatcg cgtcatccag
    10261 tgcaaaccag cggggacgaa cgggcggctc tccgtccgga tcgatcgcaa tgacttccag
    10321 gtagcagcgc gccccaagcc tgagcagggc gttgtgcgtg cccatcgccg cgtgctgccc
    10381 gcccggctgg ggccgtacga ccaggtgctg cccgacccat tcgatgccgt catcaaggcg
    10441 ggtgcttgcc accacgaggt gatcgagttc ggaacgttgc atgtcgcgat tctagggaag
    10501 tgcgcggtaa cgagaggtca gggcgaaaca caccggtgca ggtgtagggc gtcaaagttg
    10561 ctgagacgct gatcgctggg aatacaacaa cttggcgacc aacgggtaac tagacgatca
    10621 gggcttgtgt caaagtaatt ggcacctgcc tacaccccgg tcagagttac tgaatccgcg
    10681 tacacacact gcacgcccag ggtcagcttc ctgaaacgcg ccgtgcgccg tcgacccttt
    10741 gctgacatga agcccgccgc atagcggaca cgagttcccc gccgacagca gacgttgttg
    10801 ctgggcggcg ccgattattg ctccggcgtc cgtccccagg tctgaaggaa tagagaggag
    10861 acgaccagtg tggagggatt ctccaggtgc tgccttagtg ccgccatgag ttcatcgagt
    10921 tcagctaccg caatcagctc cttctccagg attcgagtcc gcgcgttctc gacgaattcg
    10981 agcaccagca tgcgacgtgc gtgtccgagg ggatacacgt gaaccagagg cttcacacgg
    11041 atatcgagga ggccgaactc tcgcagcatg cgcggcattc ggagaccgat ggaccgatca
    11101 atgccgttca tctccgcata gctgttcagg atctgcaaga gtcgcgtctg cgcggccaga
    11161 ggcggatcgc atcgctgcgt cgttgagtcc ggctcatgca gagcgatgac gccgccaggc
    11221 ctcagaagac ggatcatctc tgcaacgatc tcctccggtc gcggcacatt cacaagaacc
    11281 aggcgcgccg ttgcgagatc gaacgtcttc tcgggaagtg ctgtggcgcg agcgtccgca
    11341 tcaaggactt ccacgttcat caactggctc tgggagacga actgacgggc gcgttccact
    11401 tgttcggtac ttcgctccac cccgactacc cttcccgagg caccgacgcg cttggagaga
    11461 agtccgaggc agccccgtgg gccgcagccg atctcgacca ctcgccatcc ctcacgaacg
    11521 ccaatctgct cgaaaagccc ctctgattcg tgagcgagct ggagcgattg ctgctccagt
    11581 cgttcttgct cggcctgtcg gtagccgaga agatacggat ccggagtgct cactttgatg
    11641 gtccagaagt gatcaaaccg gtcttcctgg ctatctcgcc tgcccgcttg aactccgtgc
    11701 ggatctcggc cgcggtctgc tcgggtgtgg cgtaactctg caagatgccg agcgatgcca
    11761 gtcttgacgc gatagcgggt gcattcaccg cctgctcaat cgcgccgaca agggccgtcc
    11821 tcgcgtcttc ggggattccg gcaggcgcga ggaagctgaa ccagatgccg aacagttcct
    11881 cttgatagcc gagctcgcgc atcgtgggta tgtcgacaaa ttcgggattc cggctcgaag
    11941 cgacgaggcc ccggagcgcc cctgacttga ggtgggcgct cagtgcaccg agcgacagca
    12001 cgacaccttc gatgtgtctt ccgcgcaaag cgatgatggc gggcgtagct cccgcatagg
    12061 gaattggcac gagctctgta ccggtgagcg cattgatcaa ctgtacgcag aagtcaccga
    12121 ccgagccgga gccaggatga ccaatatgca cctggccagg gttcttcctc gagtattcga
    12181 tcaactccgg gagagttcta tacggtgcat cgctctgcac gacgaggatg gtcggcgtgc
    12241 gcgaggcggt gccgagtggc accagatccc ggagggcatc gtaggcgact gattttgggt
    12301 cgagaacggt acgaaaggtc agtgcgctat tttgcgcaaa caggatcgtg taaccgtcct
    12361 ttctggcctg gacgacgctg ttggtgccga ccgcgccccc ggcgccgggc cgattgatcg
    12421 taagaactgg tgtcttcagc agcctcgaga tctctacgcc aagggtgcgc gcggcaatgt
    12481 cggccgcgtc acccggggca agcggaacca cgagacttat cgggcc
//
LOCUS       DATIRT010000194         3371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1502587, whole genome shotgun sequence.
ACCESSION   DATIRT010000194 DATIRT010000000
VERSION     DATIRT010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502587"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1453)
                     /locus_tag="VLN25_04655"
     CDS             complement(<1..1453)
                     /locus_tag="VLN25_04655"
                     /inference="COORDINATES: protein motif:HMM:NF039580.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HSQ09893.1"
                     /translation="MSTTVAVPLWLALLIAVLAAFALLDRLLVPSARWFLRSRANKVL
                     EEVGTHLKIQVRPFQQSSRRALIDRLVYDDKVQQAARQYAAAHAMPVEVAVKIVESHA
                     REIVPAFNAYFYFRVGYWLGKSVSRALYRVRIGYADDAGLAAVPDNATVVFVMNHRSN
                     MDYIIASYLVAEKAALSYAVGEWARVWGLQQLIRSMGAYFVRRNSRDELYRRVLERYI
                     AMATEAGVTQAVYPEGGLTRDGRMREPKFGVLDYMLRGMTTDGERDLVFVPVGLNYDR
                     TLEDRTLLLGDSDAPRPGPMKAVATTLRFFAQQLGLVLHSRWYRFGYACVNFGTPVSV
                     RAYAAERGIDFRRLPKDDRSRAVAELGHRLVDDVRRLIPVLPVPLVATVVLRAVRPLS
                     EFELKSAVAALVHELEAAGAQVYVPRSDWDYAVGAGLRMLVLRRLVSESDGLYAAQPS
                     EERLLRYYANSIEHWLPPLAAGAASIDGIARPVA"
     gene            complement(1604..2488)
                     /locus_tag="VLN25_04660"
     CDS             complement(1604..2488)
                     /locus_tag="VLN25_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HSQ09894.1"
                     /translation="MPVVAVINRKGGSGKSTLATHLAAHFAHGGAAVMLGDADHQQST
                     QTWLRQRRSHQLGQRREIIGWNIDPRSFARPPSGIDHVVVDTPGGLRGLDLARVVMYA
                     DAILMPVCNSIFDRESAADCLAELRQLPRIASGRCKVAVVGMRLDARTKSAEYLQEWA
                     AKLGLPFIAVLRESQLYVRCIEKGLTLFDLPAMQVQADLAQWEPILKWLQSSLNPAPR
                     ALEDISHAAPAHGVPQRAPAGVAATRAPIPMATPVLSARRPNESSARVAPRQAGSASP
                     MSRLLDALPIPRFLQRTP"
     gene            complement(2740..>3371)
                     /locus_tag="VLN25_04665"
     CDS             complement(2740..>3371)
                     /locus_tag="VLN25_04665"
                     /inference="COORDINATES: protein motif:HMM:NF013935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HSQ09895.1"
                     /translation="RREASARRHRDCMSDLTPTRSLLIALADERRTMREGYAFLDEKC
                     LLLAGEMLRQVRLHQSLARQIVALNSAARHTLGSALARHGLQGLQVYPAGLSRQRLRI
                     EPRPLLGVMTLSAQVVGETATAPPAVNPSPEAEACRVTYARVAARLAELAAVSATLMR
                     LYSEYRRTVRRVRALQDVLLPELDADIADIDTRLEDIEREDAISMRFRR"
BASE COUNT          580 a         1096 c         1162 g          533 t
ORIGIN      
        1 gagcaacagg acgagcaatc ccgtcgatcg aggcggcccc cgcggctaga ggcggcagcc
       61 aatgctcgat cgaattcgcg tagtagcgca gcagacgctc ttcagacggt tgtgcggcat
      121 acaggccgtc gctttcgctc accaggcgtc gcagcaccag catgcgcagg cccgcgccga
      181 cggcatagtc ccagtcgctg cggggaacat aaacctgcgc gccggccgct tcaagctcgt
      241 gcacgagggc ggcgaccgcc gacttcagtt cgaattccga cagcggccga actgcacgca
      301 gcaccaccgt ggccaccagc ggaaccggaa gaaccgggat caggcgccgg acatcgtcga
      361 ccagacgatg gccgagctcg gcgacagcgc ggctgcggtc gtccttcggg agtcgccgga
      421 agtcgattcc gcgctcggcc gcatacgcgc gcacggacac gggcgtgccg aagttcacac
      481 aggcgtagcc gaagcgatac cagcggctgt gcagcacaag gcccagctgc tgcgcgaaga
      541 aacgcagcgt ggtcgcgacg gccttcatcg ggccgggccg cggcgcatcg ctgtcgccca
      601 ggagcagcgt gcggtcctcg agcgtccggt cgtagttcag gcccactggt acgaagacga
      661 ggtcccgttc cccgtccgtc gtcatcccgc gcagcatgta gtcgagcacg ccgaacttcg
      721 gttcacgcat gcggccgtcc cgcgtgaggc cgccttccgg atagaccgcc tgcgttaccc
      781 cggcctccgt cgccatcgcg atgtagcgct cgagtacacg gcggtacagt tcatcgcgcg
      841 agttccgccg cacgaagtac gctcccatcg agcggatgag ctgctgcagc ccccagacgc
      901 gtgcccactc gcccaccgcg taggagagcg ccgccttttc cgcgaccagg taggacgcga
      961 tgatgtagtc catgttgctg cggtgattca tcacgaatac gaccgtggcg ttgtcgggca
     1021 cggccgccag cccggcgtcg tcggcatagc cgatgcggac gcgatacagc gcacggctga
     1081 cggacttccc aagccagtag ccgacgcgga agtagaagta ggcattgaac gccggcacga
     1141 tctcgcgggc gtgggactcg acgatcttga cggccacctc gaccggcatg gcgtgggctg
     1201 cggcgtactg gcgcgccgcc tgctgcacct tgtcgtcata gacgagccgg tcgatcaatg
     1261 cgcgccggct ggattgctgg aacggtctga cctggatttt caggtgggtc ccaacctctt
     1321 cgaggacctt gttggcgcgg ctgcgcagaa accagcgggc actcggaacg agcaggcggt
     1381 caagcagcgc gaaggccgcg agcacagcga tcaggagcgc cagccacagc ggcaccgcaa
     1441 cggtcgtcga catgaggccc ttccggggtg cgcggttcga gcagactgac ccacgcggtg
     1501 cgggcgacga tacggccact ggcgcatggg tgacaagctg cgaatcgcgg cgggaccgcg
     1561 acaaggcgcg tgcccggtac gcgcgggctt gcgggacgga gcgctacgga gtgcgctgca
     1621 ggaagcgcgg aatcggcagt gcgtcgagca ggcggctcat cggcgaagcg ctgccggcct
     1681 gtcgtggtgc gacgcgcgcc gacgattcat tgggacgccg agcagagagc acgggcgtgg
     1741 ccatcggaat cggtgcgcgg gtggccgcga cgcctgccgg cgcgcgttgc ggcactccat
     1801 gcgcaggcgc cgcgtgtgaa atgtcctcta acgctcgggg agccggattc aggctcgatt
     1861 gcagccactt caggatgggt tcccattgcg ccagatccgc ctgcacctgc atggcaggca
     1921 ggtcgaacag cgtcagtccc ttctcgatgc agcgcacata aagttgggat tcgcgaagta
     1981 cagcgatgaa tggcagcccg agcttcgctg cccattcctg caggtactcg gccgacttgg
     2041 tgcgcgcgtc cagccgcatg ccgacgaccg caaccttgca tcggccgctc gcaatacggg
     2101 gcaactgtcg cagttcggcg aggcagtctg cggccgattc gcggtcgaag atcgaattgc
     2161 agaccggcat caggatcgcg tctgcgtaca tgacgacacg tgcaagatcc agcccgcgca
     2221 gaccgcccgg tgtatcaact acaacgtggt cgatgcctga cggcgggcgt gcaaaggagc
     2281 gcggatcgat gttccagccg atgatttccc ggcgctggcc gagctgatgc gagcggcgtt
     2341 gccgcagcca cgtctgggtc gattgctggt gatcggcgtc gccgagcatc accgccgcgc
     2401 cgccgtgggc aaagtgggcc gccaggtgcg tggccagcgt gctcttgccg cttccacctt
     2461 tgcgattgat gacggctacg acgggcatgt cggcttcttc tcgaatacat ccaaccgaac
     2521 ggcttgtcgg tcccggtttc cggctcggtc ggcgcgagca cggatgactc cttagggcat
     2581 tccagcacag cgcggatcgg tccacaccgc ggagaagccg gtggaatccg gcgcatctaa
     2641 gcgtcgagag ccgtccgccc gggtcgggag tcgtgctgcg gcagcgcaca ggcggggcgg
     2701 tggctgcagt cgggtctgcg gtccggacgc tcttccgctt cagcgtcgga agcgcatcga
     2761 aatcgcgtcc tcgcgctcga tgtcctccag cctcgtgtcg atgtcggcga tgtcggcatc
     2821 aagttcgggc aacagcacgt cctgcagcgc gcgcacgcgg cgtaccgttc ggcggtattc
     2881 ggagtacagc cgcatcagcg tggccgagac cgcggccagc tcggcgagcc gggccgcaac
     2941 gcgtgcgtag gtgacgcggc aggcttcggc ctccggagag gggttgaccg caggcggggc
     3001 cgttgcggtt tcaccaacca cctgcgctga cagcgtcatc actcccagca agggacgtgg
     3061 ctcgattcga aggcgctggc gtgagagccc ggccgggtaa acctgcaggc cctgcagtcc
     3121 atgccgtgcc agagcggagc cgagcgtgtg tcgcgcggca ctgttcagag cgacgatctg
     3181 tcgcgcgagc gactgatgca gacgcacctg ccgcagcatt tcacctgcca gcagcaggca
     3241 cttttcgtcg aggaaagcgt aaccctcgcg catcgtgcgt cgctcgtctg cgagcgcgat
     3301 cagcaggctg cgggtgggag tgaggtcgct catgcagtcg cggtggcgac gggcggacgc
     3361 ttcacgacgg g
//
LOCUS       DATIRT010000195         3774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_421333, whole genome shotgun sequence.
ACCESSION   DATIRT010000195 DATIRT010000000
VERSION     DATIRT010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421333"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="VLN25_04670"
     CDS             <1..1099
                     /locus_tag="VLN25_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HSQ09896.1"
                     /translation="RGLAMCRRNWEATAKKGRMPAEEVDKRLGLLRPTLDFTSLGSAD
                     IVIEAVYEDMVVKQDIFARLDAVMKRGAILATNTSTLDVDRIAAATSRPHDVIGTHFF
                     SPANVMRLLEVVRAKKTGTDVLATVMQLARRLKKVGVVSGVCDGFIGNRMLEQYIRQS
                     LFLIDEGASPQQIDAALTKFGMAMGPFAMYDMAGMDIGYAIRQRRYVDKPHLTYSRIG
                     DRVVELGRLGQKSGKGWYRYEPGSRTPLPDSEIDALIERYRTEIGMRPRAIGDEEIVQ
                     RCIYALVNEGARILAEGIALRASDIDVVYLAGYGFPVARGGPMFHAQAVGLDKVVAAM
                     ARFAANKHADPAFWQPAPLLVEAAKSGRWPK"
     gene            1105..2964
                     /locus_tag="VLN25_04675"
     CDS             1105..2964
                     /locus_tag="VLN25_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HSQ09897.1"
                     /translation="MELIVTRRDLSFLLYEWLDVETLTQRPRYSAHARETFEAALDTA
                     EQIATDLFAPHARKGDLNEPHFDGERVQLVPEVKQALDAFVAAGLMAAEHDEEFGGMQ
                     LPIAVAKAAFAYFKGANVASSGYAMLTVGNANLLLAHGSPAQIDAFVRPALAGRFFGT
                     MCLSEPQAGSSLSDITTRAEFERDSPLGAQYRLAGNKMWISAGEHELSENIVHLVLAK
                     IPGPDGKPIAGTKGISLFVVPKYLVGVEGEGAVSRVTVGERNDVVLAGLNHKMGYRGT
                     SNCLLNFGEGKFRPEGRAGAVGYLVGEPGRGLAAMFHMMNEARIGVGLGATMLGYTAY
                     LHALEYARGRPQGRPIGPAGKDPAQSQIPIVQHADVKRMLLAQKAYAEGALALTLYCA
                     RLIDDQKCAESEDVRAEAELLLDILTPIAKSWPSQWCLEGNALAIQVHGGYGYTRDYM
                     VEQFYRDNRLNSIHEGTHGIQALDLLGRKVVMREGAAFRLLGERMDDTIRRASHARHP
                     DTQRWAAMLGDRVKRVAQVTQRAWAAGDPARTLANATAYMEAFGHVVLAWIWLEQSLA
                     ASKHYQRAEGNFYHGKWQACRYFFLYELPKVDAMLELVESLDTTTLDMQDLWF"
     gene            3024..>3774
                     /locus_tag="VLN25_04680"
     CDS             3024..>3774
                     /locus_tag="VLN25_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSQ09898.1"
                     /translation="MALKQLLDLTGRTALITGGSRGLGLQIATGYGEMGARVLISARK
                     TDELDAACTQLRAQGIDASWIAADAAREDELTRLAREAVARLGPIDILVNNAGATWGA
                     PAEDHPLEAWDKVMNLNIRGVFLLTQAIGKACMIPRRYGRIVNVASIAGLLGNPPKLM
                     STIAYNTSKGALVNFTRALASEWGRHNITVNALAPGFFPSKMTQGVIAHFGAEALARF
                     APLERIGDDEDLKGAALLFASEAGKHITGQIL"
BASE COUNT          685 a         1169 c         1278 g          642 t
ORIGIN      
        1 tcgcggcctg gccatgtgcc gccgcaactg ggaggcgacc gcgaagaagg gccgcatgcc
       61 ggcggaggag gtggacaagc gcctcgggct gctgcggccc acgctggact tcacctcgct
      121 cggcagtgcc gacatcgtca tcgaggcggt ctacgaggat atggtcgtca agcaggacat
      181 ctttgcccgg ctcgatgccg tgatgaagcg tggcgcgatc ctggccacca acacgtcgac
      241 gctcgatgtc gatcgcattg ccgccgcgac gtcgcgcccg cacgatgtca tcggcacgca
      301 ctttttcagc ccggcgaacg tgatgcggct gctcgaggtg gtgcgcgcga agaagaccgg
      361 caccgacgtg ttggccaccg tcatgcagct ggcgcggcgc ctgaagaagg tgggcgtggt
      421 ctcgggtgtg tgtgacggct tcatcggcaa ccgcatgctc gagcagtaca tccgccagtc
      481 actgttcctc atcgacgagg gtgcctctcc ccagcagatc gacgcggcac tcacgaagtt
      541 cggcatggcg atgggcccgt ttgcgatgta cgacatggcg ggcatggaca tcggctatgc
      601 catccgccag cgccgctatg tcgacaagcc gcatctgacg tacagccgca tcggcgaccg
      661 tgtcgtcgag ctcggacggc tcgggcagaa gtccggcaag ggctggtacc gctacgaacc
      721 cggcagccgc accccgctcc cggattcgga gatcgatgcc ctgatcgaga ggtatcgcac
      781 ggagatcggg atgcggccgc gtgcaatcgg cgacgaagaa atcgtgcagc gctgcatcta
      841 cgcgctcgtc aacgagggtg cgcgcatcct cgccgagggg atcgctctgc gtgccagcga
      901 catcgatgtg gtgtacctcg ccggttacgg ctttcctgtc gcccggggcg gtcccatgtt
      961 ccacgcgcag gccgtgggac tggacaaggt cgttgccgca atggcgcgtt ttgcagccaa
     1021 caagcatgcc gatccggcgt tctggcagcc ggcgccattg ctggtcgaag ccgcgaagtc
     1081 cggccgctgg ccaaagtagt gaccatggaa ctcattgtta cccggcgtga tctttcgttt
     1141 ctgctgtacg agtggctcga tgtcgaaacg ctgacgcaac ggccgcgcta ttccgcgcac
     1201 gcgcgcgaga ccttcgaggc ggcactggac acggccgagc agatcgcgac ggatctcttc
     1261 gccccgcacg cacgcaaagg cgacctgaac gagccgcact tcgacggcga gcgcgtgcaa
     1321 ctggttccag aggtcaagca agccctggac gcctttgtcg cggcgggact gatggcggcc
     1381 gagcacgacg aggagttcgg tggcatgcag ctgccgattg ccgtggccaa ggctgccttc
     1441 gcctacttca aaggcgccaa tgttgctagt tcgggctacg cgatgctgac ggtggggaat
     1501 gccaacctgc tgctcgcaca cgggtcaccg gcgcagatcg atgcattcgt gcggccggca
     1561 ctggcgggcc gattctttgg cacgatgtgc ctgtcagagc cgcaggcggg ttcgtcgctg
     1621 tcggacatca ccacccgcgc ggaattcgaa cgcgactcgc cactcggcgc gcagtatcgg
     1681 ctggccggga acaagatgtg gatttcggcc ggcgaacacg aactgtccga gaacatcgtg
     1741 cacctggtgc tggcgaagat ccccgggccg gatggaaaac cgatcgccgg gacgaagggc
     1801 atatcgctat ttgtggtgcc gaagtacctc gtcggtgtcg agggtgaggg cgccgtatcg
     1861 cgcgtgaccg tcggcgagcg caatgacgtc gtgcttgccg gactgaacca caagatggga
     1921 taccgcggga catccaactg cctgctgaac tttggcgagg gcaagttccg tccggaaggg
     1981 cgggctgggg cggttggata tctcgtgggc gagccggggc gcgggctcgc cgcgatgttt
     2041 cacatgatga acgaggcgcg gatcggcgtg ggcctcggcg caacgatgct gggttacacc
     2101 gcctacctgc acgcgctgga gtacgcgcgt ggccgtcccc agggccggcc catcgggccg
     2161 gcgggcaagg acccggcgca gtcgcagatt cctatcgtcc agcatgcgga tgtgaagcgc
     2221 atgctgcttg cgcaaaaggc ctacgcggaa ggcgcactgg cgctcacgct ttactgcgcg
     2281 cgcctcatcg atgaccagaa gtgcgccgag tccgaggacg tgcgcgccga ggcggagttg
     2341 ctgctcgaca ttctgacgcc gatcgccaag agttggccgt cgcagtggtg ccttgaaggc
     2401 aacgcgctgg cgatccaggt ccacggcggc tatggctaca cgcgcgacta catggtcgag
     2461 cagttttatc gcgacaaccg gctgaattcg atccacgaag gcacgcacgg catacaggcg
     2521 ctcgatctgc tgggacgcaa ggtcgtcatg cgtgagggag cggcattcag gctgctcggt
     2581 gaacggatgg acgacacgat ccggcgtgcc tctcacgcac gccatcccga cacgcaacgc
     2641 tgggcggcga tgctcggcga tcgcgtaaag cgggtggcgc aggtgactca gcgtgcctgg
     2701 gccgcaggtg acccagcgcg cacgctggcc aacgccacgg cctacatgga ggccttcggc
     2761 catgtggtgc tcgcgtggat ctggctcgag cagtcactag cggcctcgaa gcactaccag
     2821 cgggccgagg gcaacttcta tcatggcaag tggcaggcat gccgttattt cttcctctat
     2881 gaactgccga aggtcgacgc aatgctggag ctggtggagt cgctggacac gaccacactc
     2941 gacatgcagg acctgtggtt ctgatggaag ggcacgacgg gaagggaacg acctgacgcc
     3001 tgcgggcgct acatgaggca accatggcac tgaagcagtt gctcgatctc accggcagga
     3061 cggcgctcat cacgggtgga tcgcgcgggc ttggcctgca gatagcaacc ggttacggcg
     3121 aaatgggcgc ccgcgtgctc atttccgccc gcaagaccga tgagctcgac gcggcctgta
     3181 cgcagttgcg cgcgcaaggc atcgatgcga gctggatcgc agcggatgcg gcgcgcgaag
     3241 acgaactcac ccggctcgca cgggaggccg tggctcgtct cggccctatc gacatactcg
     3301 tgaacaatgc cggcgcgacc tggggtgcgc cagcggaaga tcatccgctc gaggcctggg
     3361 acaaggtgat gaacctgaac attcgcggtg tgttcctcct gacgcaggcc atcggcaagg
     3421 catgcatgat cccgcgccgc tacggacgta tcgtcaacgt tgcatcgatc gccggactgc
     3481 tcggcaatcc gcccaagctc atgagcacga ttgcgtacaa caccagcaag ggcgcgttgg
     3541 tcaacttcac gcgcgccctg gcgagcgagt ggggccggca caacatcacg gtcaatgcgc
     3601 tggcaccggg gttctttcca tcgaagatga cccagggggt catcgcgcac ttcggagccg
     3661 aggcgctcgc ccgctttgcg ccgctggagc gaattggcga cgatgaggac ctgaaaggtg
     3721 cggcgctgct gttcgcttcc gaggccggca agcacatcac tggccagatc ctgg
//
LOCUS       DATIRT010000196         3111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1207935, whole genome shotgun sequence.
ACCESSION   DATIRT010000196 DATIRT010000000
VERSION     DATIRT010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207935"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VLN25_04685"
     CDS             <1..339
                     /locus_tag="VLN25_04685"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HSQ09899.1"
                     /translation="SINGLQTLGENISDLAGVKIAFDGLQIALARQPIGPIDGLTQNQ
                     RFFISYATIWRGQYRTEALLDQLRTGQHSPHRYRVLGPLANFPAFAAAFTCPANAPMM
                     RSPADQISIW"
     gene            complement(340..756)
                     /locus_tag="VLN25_04690"
     CDS             complement(340..756)
                     /locus_tag="VLN25_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09900.1"
                     /translation="MSTPAPLNTPNESLVTTTHIVYALHTLGLVIGAFGTASIIGAFL
                     FGWPSIIAVIINYVKRSEVRGTWLDSHFSWQIRTFWWALAIAVVVLLVGLVLLPALGL
                     GLVVWWIGFFALGIWAIYRIARGWLLLKDRQPMPAP"
     gene            complement(873..2261)
                     /locus_tag="VLN25_04695"
     CDS             complement(873..2261)
                     /locus_tag="VLN25_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HSQ09901.1"
                     /translation="MTTRRTTISASRRTFLRQASALSLAGTAAPFALNLASIGAASAS
                     TAADYKALVCVFLYGANDAHNTVVPYDATGYATYAGVRGSLAWTQAQLTPLVPTTPLP
                     GGRQVALPAELTPLATLFDAGQCAVIANVGPLIVPTDRTSFSAKSVPLPPKLFSHNDQ
                     QSVWQASVPEGAKFGWGGRIGDLMAGQNSNQIFTCNSLTGNAVFLSGQAVQPFQIDPL
                     QGSIAFSALNANSLYGSTAGAAALRNALTRPRTHLFESDLRNVNQRSIDSNTALAAAL
                     AMTPTLATPFPASNTLATQLAMVAKIISVSAELGAKRQVFMVSLGGFDTHSGQDSAHP
                     ALLTQLADSIDAFQKAMVELNAAQKVTLFTASDFGRTLTVNGDGTDHGWGGHHFVVGG
                     AVNGKQVVGSYPDLTANGPTDAGNGRLIPTTAVEQYAATLARWMGVSATDIPLILPNV
                     VNFPTADLGFMA"
     gene            complement(2272..>3111)
                     /locus_tag="VLN25_04700"
     CDS             complement(2272..>3111)
                     /locus_tag="VLN25_04700"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HSQ09902.1"
                     /translation="PQFHETGTKTFLNVTIPANTSAQASLTIALDTLFNHPNLCPFIG
                     RQLIQRLVTSNPTPAYVGRVSTACTDNGAGVRGDMKAIVRAILLDSEARSSAGLADPQ
                     FGKLREPVLRLSQWARCFGATSTSGNWQIRNLDSSATSLGQQPLRAPSVFNFYRPGYV
                     PANTAIASAGLVAPEFQITGETSVAGYTNFMQAAISSGVGVSREVRASYTTELAVADN
                     ADALIDRLNRCLMAGTMGTQQRQEIRDAVNAIAVTSPNGRANRVYTAVLLTMASPEYL
                     VQK"
BASE COUNT          516 a          983 c         1099 g          513 t
ORIGIN      
        1 tcgatcaatg gcttgcagac gctgggcgag aacatctcgg atctcgccgg cgtgaagatc
       61 gccttcgacg gcctgcagat cgccctggcg cgccagccga tcggcccgat cgacggcctg
      121 acgcagaacc agcggttctt catttcctac gcgacgatct ggcgggggca gtaccggacc
      181 gaggcgctgc tcgaccagtt gcgcaccggc cagcattcgc cccatcgcta tcgcgtgctg
      241 gggccgcttg ccaattttcc ggcgttcgcg gcagcgttca cctgcccggc gaacgcgccg
      301 atgatgcggt cgcccgccga tcagatctcg atctggtgat cagggcgcgg gcatcggctg
      361 ccggtccttc agcagcagcc agccgcgcgc aatgcgatag atcgcccaga tgccgagcgc
      421 gaagaagccg atccaccata cgacgagtcc gagcccgagt gcgggcagca aaacgagccc
      481 gaccagcagg accacgacgg caatggcgag cgcccaccag aaggtgcgga tctgccatga
      541 gaagtgcgag tcgagccagg tgccgcgcac ttcgcttcgc ttcacgtagt tgatgatcac
      601 cgcaatgatc gaaggccagc cgaagagaaa ggcgccgatg atcgaagccg tcccgaacgc
      661 gccgatcacc aggccgagcg tgtgcagcgc gtagacgatg tgcgtcgtcg tcaccaacga
      721 ttcgttgggc gtattcagtg gcgcaggcgt gctcatcggc ggctccgtca ggcaagggag
      781 cgcagcatag cgccgccgcg cgcggcggcg gaagcgggac tcaatggagc ctccgcgctg
      841 cgcgctgcgg ctcgagcttg ccaacgctgc gctcaggcca tgaagccgag gtcggccgtc
      901 gggaagttca cgacgttcgg caggatcagc gggatgtcgg tggcgctcac ccccatccag
      961 cgcgccagcg tcgccgcgta ctgctcgacc gcggtggtgg gaatcagccg gccgttgccc
     1021 gcgtcggtcg ggccgttcgc cgtcaggtcg ggatagctgc cgacgacctg cttgccattg
     1081 accgcgccgc cgacgacgaa gtggtgcccg ccccagccgt ggtcggtgcc gtcgccgttg
     1141 accgtcagcg tgcgcccgaa gtccgatgcg gtgaagagcg tcaccttttg cgccgcgttc
     1201 agttcgacca tcgctttctg aaacgcgtcg atcgagtcgg ccagctgcgt cagcagcgcc
     1261 ggatgagccg agtcctgccc ggagtgcgtg tcaaagccgc cgagcgacac catgaagacc
     1321 tgccgctttg cgccgagttc ggcgctgacg ctgatgatct tcgcgaccat cgccagctgg
     1381 gtcgcaagcg tgttactggc ggggaatggg gtggcgagcg tcggcgtcat cgccagcgcc
     1441 gcggcgagcg cggtgttcga atcgatcgag cgctgattca cgtttcgcag gtcgctttca
     1501 aacaggtggg tgcgcggacg cgtcagcgcg ttgcgcagcg cggcggcgcc tgcagtcgag
     1561 ccatagagtg agttggcatt cagcgcactg aacgcgatcg agccctgcaa ggggtcgatc
     1621 tggaacggct gcacagcctg gcccgacagg aaaactgcat tacccgtcag cgagttgcag
     1681 gtgaagatct gattcgagtt ctggcccgcc atcaggtcgc cgatgcgtcc gccccagccg
     1741 aacttcgcgc cctcgggcac cgacgcctgc cataccgact gctggtcatt gtgcgagaaa
     1801 agtttcggcg gcagcggcac cgacttagcg ctgaagctgg tgcggtcggt cggcacgatg
     1861 agcgggccga cgttggcgat gacggcgcac tgaccggcgt cgaagagcgt cgcgagcggc
     1921 gtcagttcag ccggcagcgc gacctgccgg ccgccgggaa gcggggtggt tggaacgagc
     1981 ggcgtcagct gcgcctgcgt ccaggcgagc gaaccgcgca cgcccgcgta ggtcgcgtag
     2041 ccggtcgcgt cgtacgggac gacggtgttg tgcgcgtcgt tcgcgccgta caggaagacg
     2101 cagaccagcg ccttgtagtc ggccgccgtg gatgcggatg cggcaccgat cgacgcgagg
     2161 ttcagcgcaa agggggcagc ggtcccggcg agcgagaggg cggatgcctg gcggagaaag
     2221 gtgcggcggg aagcgctaat ggtcgtcctt cgggtcgtca tcacgggtct cctacttctg
     2281 caccaggtac tcgggcgagg ccatcgtcag cagcaccgcg gtgtagacgc gattggcgcg
     2341 accgttgggc gacgtcacgg caatcgcgtt gacggcgtcg cggatctcct ggcgctgctg
     2401 cgttcccatc gtgccggcca tcaggcagcg attaaggcgg tcgatcagcg cgtcggcgtt
     2461 gtcggccacc gcgagctcgg tggtgtagga ggcgcgcact tcgcggctga caccgacgcc
     2521 gctcgagatc gcggcttgca tgaagttggt gtacccggcg accgaggtct caccggtgat
     2581 ctggaattcc ggtgcaacca ggccggcgga cgcgatcgcc gtgttggcgg gcacataacc
     2641 gggacggtag aagttgaaga ccgacggcgc gcgcagcggc tgctgcccca gcgacgtggc
     2701 cgaactgtcg aggttgcgga tctgccagtt gccggaagtc gaggtcgcgc cgaagcagcg
     2761 cgcccattgc gacagtcgca gcacgggttc gcgcagcttg ccgaactgcg ggtcggcgag
     2821 gccggcactg ctgcgcgctt cgctgtcgag caggattgca cgcacgatgg ccttcatgtc
     2881 tccgcgcacg ccggcgccgt tgtcggtgca ggcggtcgac acccggccga cgtaggccgg
     2941 cgtcggattg ctcgtcacca gtcgctggat cagctgcctg ccgatgaacg gacacaggtt
     3001 cggatgattg aagagcgtgt ccagcgcgat cgtcaggctc gcctgcgccg acgtgttcgc
     3061 gggaatcgtc acgttgagga aggtcttggt tccggtttca tggaactgcg g
//
LOCUS       DATIRT010000197         2225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_27637, whole genome shotgun sequence.
ACCESSION   DATIRT010000197 DATIRT010000000
VERSION     DATIRT010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27637"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VLN25_04705"
     CDS             complement(<1..546)
                     /locus_tag="VLN25_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HSQ09903.1"
                     /translation="MSIRWFAITAVALLVAGCDTLSKGPFAEAKVLGTDGSSVWGRVT
                     FHQAEGGTFVADGTGYRAPLASAAADSAAGKTFIRADINGLPANRQFGFHVHEKGDCS
                     DNGMGAGGHFNPQAKPHGRYDATDRHAGDLPNLSSNGEGVAMFAFTTELLVVAPGPRS
                     VVNRAIVIHESADDFKSQPAGN"
     gene            697..1203
                     /locus_tag="VLN25_04710"
     CDS             697..1203
                     /locus_tag="VLN25_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HSQ09904.1"
                     /translation="MPFCRFPLVVLLVLLLQSPATCAVEWVGTVVGIADGDTLTLLDD
                     GKTPHRIRLDGIDAPERLQGHGQRARQSLADLAHGRRAVAACPKTDRYGRAVCRVTVD
                     GVDVGLEQIRRGLAWHYVKYANEQSPTDRMRYAGAEEQARSTRQGLWSTSAAVPPWDF
                     RRGKAATK"
     gene            complement(1229..1657)
                     /locus_tag="VLN25_04715"
     CDS             complement(1229..1657)
                     /locus_tag="VLN25_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HSQ09905.1"
                     /translation="MLVRLLYASRAAAPVTQEIIESILQQSRTHNPAHGITGILCHGG
                     DVYMQALEGGREAVNALYIKIVRDTRHREVILLHYSEVPERHFAGWTMGQVNLSKVNP
                     SMLLKYSDKPVLDPFTVSGRASMALLEELIASAQIVGRAG"
     gene            complement(1671..>2225)
                     /gene="folE"
                     /locus_tag="VLN25_04720"
     CDS             complement(1671..>2225)
                     /gene="folE"
                     /locus_tag="VLN25_04720"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HSQ09906.1"
                     /translation="DKLLGVLQSLVIDVEGDHNTRETARRVAKMYLTEVFRGRYTAMP
                     AVTEFPNVERLNELMIVGPITVRSACSHHLCPIIGKVWIGVLPNEYSNLIGLSKYVRL
                     ADWVMSRPQIQEEAVTHLADLLQNTMQPDGLAIVMEADHFCMQWRGVKDTDSKMINSV
                     MRGSFLKDPDLRREFLSLLKLKNA"
BASE COUNT          376 a          711 c          737 g          401 t
ORIGIN      
        1 gttgccggca ggctggctct tgaaatcgtc ggcgctctca tgaatgacga tcgcccggtt
       61 cacgaccgag cgcggccccg gcgccaccac caggagttcc gtcgtgaacg cgaacatcgc
      121 gaccccttct ccgttcgatg agaggttcgg caggtctccg gcatggcggt cggtcgcgtc
      181 gtagcgtccg tgaggcttgg cctgcggatt gaagtgtccg ccggcgccca tgccgttgtc
      241 ggagcagtcc cctttttcgt gcacatggaa gccaaactgg cggttggccg gcaaaccgtt
      301 gatgtcggca cggatgaaag tcttgccggc ggctgagtcc gcagccgccg aggccagggg
      361 cgcccggtaa ccggttccgt cggcgacgaa ggtcccgcct tcggcctgat gaaacgtcac
      421 gcgcccccac acggagctgc cgtcggttcc gaggaccttg gcttccgcga acggcccctt
      481 gctcaaggtg tcgcagcccg cgacgagcag ggcaacggcg gtgatggcaa accagcggat
      541 cgacatcgag gattccttgc gtgagaaagc gcccgggagg cgagccgtga tgttagccgc
      601 cggagcccgg aattgcagcg ccggggtccc gctttccatc tcactgcgcg cgcgccacgt
      661 tcctagcatc ggtggcatca acggctcgct tgtcccatgc ctttctgtcg attccctctg
      721 gtcgtgctgc tcgtgctgct gctgcaatcg ccggccacct gtgcggtcga gtgggtcggc
      781 accgtggtcg gcatcgccga tggcgacacg ctcaccctgc tcgatgacgg caagactccg
      841 caccgtatcc gactggacgg catcgacgcg ccggaacgac tgcagggcca tggacagcgc
      901 gcccgccagt cgctcgccga cctggcgcac gggcgccgcg cggtcgccgc atgccccaag
      961 acagaccgct acggtcgggc ggtgtgtcgc gtgacggtgg atggcgtgga tgtcggtctc
     1021 gaacagatcc ggcgcgggct cgcctggcac tacgtcaagt acgcgaacga acagtcgccg
     1081 accgatcgca tgcggtatgc gggagccgaa gagcaggcgc gcagcacgcg ccagggcctg
     1141 tggtcgacgt cggctgcggt gccgccgtgg gatttccgtc gcggcaaggc ggcgacgaag
     1201 taaggtgatt cagcccaggt gaggcgactc agcccgcgcg gccgacgatc tgcgcagagg
     1261 caatcagctc ttccagcagc gccatcgacg cccgccccga gacagtgaaa ggatcgagca
     1321 ccggcttgtc ggaatacttc agcagcatcg acgggttgac cttcgacagg ttcacctggc
     1381 ccatggtcca gccggcgaag tggcgctccg gcacctcgga atagtgcagc aagatgacct
     1441 cgcggtgacg cgtgtcgcgc acgatcttga tgtacagcgc gttgaccgct tcgcggccgc
     1501 cttccagtgc ctgcatgtag acatctccgc cgtggcacag gatgccggtg atcccgtggg
     1561 cgggattgtg agtgcgcgac tgctgcagga tcgactcgat gatttcctgg gtgacggggg
     1621 cggcagcacg gctggcgtac agcaggcgga caagcattga ggatctccgg tcaggcgttc
     1681 ttcagtttca gcagtgacag aaactcgcgc cgcaaatcag ggtccttcag gaatgaccct
     1741 cgcatcacgc tgttgatcat cttcgaatcg gtgtctttga cgccgcgcca ttgcatgcag
     1801 aagtgatcag cctccatcac gatggccagg ccgtcgggct gcatcgtgtt ctgcagcagg
     1861 tccgccaggt gcgtgacagc ctcttcctgg atctgcggcc ggctcatgac ccagtcggcc
     1921 aggcgcacgt acttcgacag cccgatgagg ttcgagtact cgttgggcag gacaccaatc
     1981 cacaccttgc cgataatcgg gcacaggtgg tgggagcagg cactgcgcac cgtgatcgga
     2041 ccgacgatca tcagttcatt gaggcgttcg acgttgggaa actcggtcac cgcaggcatt
     2101 gcggtgtagc ggccgcggaa gacctcggtc aggtacatct tcgccacgcg gcgcgccgtt
     2161 tcgcgcgtgt tgtgatcgcc ctcgacgtcg atgaccaggc tctgcagcac gccgagcagc
     2221 ttgtc
//
LOCUS       DATIRT010000198         6869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_518972, whole genome shotgun sequence.
ACCESSION   DATIRT010000198 DATIRT010000000
VERSION     DATIRT010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6869
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518972"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VLN25_04725"
     CDS             complement(<1..903)
                     /locus_tag="VLN25_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HSQ09907.1"
                     /translation="MGTYLGAALRGPLTSILPHHEGLSELRRDLHANPEIGFEEHRTS
                     ALVAERLRGYGVDEVHTGIARTGVVGIIRGRGNSDRSIGLRADMDALTMDEENEFAHR
                     SVRSGLMHGCGHDGHTTILLGAAQYLASTRRFDGTVFLIFQPGEEGFAGAKAMIDDGL
                     FERFPADSVYALHNWPALEPGRFGLNTGPMMAAADRVEIIVSGKGGHAAHPHRAVDPV
                     VVAAHIVTAAQTIVARNVGPLDAAVLSLCSIHAGNPGAFSVIPREARMVGTVRTFSAD
                     AQDLIEQRLTDLAESTAKALGAEAK"
     gene            complement(914..1897)
                     /locus_tag="VLN25_04730"
     CDS             complement(914..1897)
                     /locus_tag="VLN25_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSQ09908.1"
                     /translation="MLAFIVRRLLQAVLVMLTVALVAFLLFQYVGDPVSQMLGQDATP
                     EDRTRLRAELGLDQPFYAQFATFVGNAAQGDFGISLRQGRAVSTLIKERLPATLELAF
                     TAAIIAIVVGIPAGVYTALRRNSWLSQALLAASLVGISLPTFLIGILLILVFAVQLGW
                     LPSYGRGDVVKVGWWSSGFLTPTGWKHLILPAITLSLFQLTLIMRLVRSEMMEVLRTD
                     YIKFARARGLTDRAVNFGHALKNTLVPVITITGLQLGGIIAFAIVTETVFQWPGMGLL
                     FIQSVQFADIPVMAAYLCLIALVFVLINLIVDLLYFAVDPRLRIDRAAAAH"
     gene            complement(2079..3655)
                     /locus_tag="VLN25_04735"
                     /pseudo
     CDS             complement(2079..3655)
                     /locus_tag="VLN25_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800630.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(3753..4508)
                     /locus_tag="VLN25_04740"
     CDS             complement(3753..4508)
                     /locus_tag="VLN25_04740"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HSQ09909.1"
                     /translation="MRFSVTRVFVPVLAMLAGCATTPAPEVPATVVARFSTNSDPGGL
                     PQGWRPWIINRTKTPTEYRLVRDPQTGVVVLHAHADAAASGLRQLLDVDPAQQPVVAW
                     RWRVLDLIVSADNQDRYAEDSPVRLMLFFDGDKKTLPFKEQVLIDTAKLLTGQDLPYA
                     TLMYIWENRFPVGTILPNSFTSQVKLLVAGSGPDRRLGKWKQFERNYVEDYRKAFGAS
                     PGRLVGIGIMTDTDNTGERIDAYYGDIELKGAP"
     gene            4926..5648
                     /locus_tag="VLN25_04745"
     CDS             4926..5648
                     /locus_tag="VLN25_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09910.1"
                     /translation="MTGFLVFAFGAGVLGAGAWLVLRARRLESDRDFERKVHARKNGW
                     TYDGRRSGRIDYRFAGTAGGIDWQMWHDSDRGDDSPTPRAFWTSANLRTPGLALVIIG
                     RKRFQLESGTVGRLLMGVVTGVAHAVTGHEGQVDKAEFYESAIEIKVGRPSFDERLTV
                     AVAPDMPRTWVDSELQALLLEWPATGTSRMFKSEDSVEVTLRADGLKIVVQKMPQDIA
                     HWRHLARLGEHIARQLSRTEPR"
     gene            5671..6375
                     /locus_tag="VLN25_04750"
     CDS             5671..6375
                     /locus_tag="VLN25_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09911.1"
                     /translation="MTDGRRQARQLAVVAGASGLVGRELVLRLLRHPVYSRVVALTRR
                     PLSIDNVRLSEVPARFESLEPTLSSAVSEPARLDAYCCLGTTIRSAGSEAAFRIVDHD
                     YVVAFGQWAVRHRAHRLVLVSALGADARGAVFYSRVKGETENALRALAPSSLVILRPS
                     LLDGDRGERRLGESFALALARPLRAVLPAAFRAVRADDVAQSMIDAALLDGAQGTIES
                     AAMHGAAARADSRTTG"
     gene            complement(6467..6838)
                     /locus_tag="VLN25_04755"
     CDS             complement(6467..6838)
                     /locus_tag="VLN25_04755"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HSQ09912.1"
                     /translation="MAGLRWGDEHRAVAEHRPLSSVALLARLFELSTPYPGDTFMVNV
                     GALSNRPEAPFSTRHAASLRAIYDLATPEASVWVQSTGQVGNPLSQLYASMLPAWRDV
                     RYFPMRPAPATAPVLLLVPLR"
BASE COUNT         1243 a         2194 c         2234 g         1198 t
ORIGIN      
        1 cttcgcttcg gcgccgagtg ccttggcagt cgactccgca agatccgtca gccgctgctc
       61 gatcaggtcc tgcgcatcgg cactgaacgt gcgcacggtc ccgaccatcc tggcctcgcg
      121 cgggatcacc gagaacgcac caggattgcc ggcatgaatc gagcacaggc tcaggaccgc
      181 cgcatccagc ggcccgacgt tgcgcgcgac gatcgtctgc gccgccgtca cgatgtgcgc
      241 ggccacgacg acgggatcga cggcccggtg cgggtgcgct gcatgcccgc ccttgccgct
      301 gacgatgatt tcgacccggt cggccgcggc catcatcgga ccggtgttca gtccgaacct
      361 tccgggttcg agcgcgggcc agttgtggag tgcgtacacc gaatcggccg ggaagcgctc
      421 gaagaggcca tcatcgatca tcgccttggc gcccgcgaac ccttcctcgc caggctggaa
      481 aatcagaaag acggtgccgt caaagcgccg cgtcgacgcc aggtactgcg cggcgccgag
      541 caggatcgta gtgtggccat cgtggccgca gccatgcatc aggcccgagc ggaccgaccg
      601 gtgcgcgaac tcgttttcct cgtccatggt gagcgcatcc atgtcggcgc gcagcccgat
      661 cgatcggtcc gaatttccac gcccgcgaat gatgcccacg acgccggtac gcgcgatgcc
      721 ggtgtgaact tcgtcaactc cgtagccgcg caggcgctcg gccaccagcg ccgacgtgcg
      781 atgttcctcg aaaccgattt ccggattggc gtgcaggtca cgccgaagct cggacagtcc
      841 ctcgtgatgc ggaaggatgg acgtcagcgg gccacgcaac gcggctccga ggtaagttcc
      901 catcggtggc gacctagtgc gccgccgcgg cgcggtcgat gcgcaggcgg ggatcgactg
      961 cgaaatacag caggtcgacg atgaggttga tcagcacgaa cacgagggcg atcaggcaca
     1021 ggtaggccgc catcacgggg atgtctgcga actgaaccga ctggatgaag agcagcccca
     1081 tgccgggcca ctggaacacg gtttcggtca cgatcgcgaa agcgatgatc ccgcccagct
     1141 gcaggccagt aatcgtgatg acgggcacca gtgtgttctt cagtgcatgg ccgaagttga
     1201 cggcacgatc ggtcagtccg cgggcccgcg caaacttgat gtagtcggtc cgcagcacct
     1261 ccatcatttc cgaacggacc agacgcatta tcagcgtcag ctggaacagt gacagcgtga
     1321 tcgccggcag gatcaggtgc ttccagccgg tgggcgtcag gaagccgctc gaccaccagc
     1381 cgaccttgac cacatcaccg cgcccgtagg acggaagcca cccgagttga accgcgaaca
     1441 caaggatcag caggatgccg ataaggaagg tcggcagcga aatgcccacg agtgatgctg
     1501 ccagcaacgc ctgcgacagc caggagttgc ggcgcagcgc ggtatacaca ccggccggga
     1561 taccgacgac gatggcgatg attgctgcag tgaacgccag ctcgagcgtc gccggcagtc
     1621 gctccttgat cagggtcgac accgcgcggc cctggcgcag gctgatgccg aagtccccct
     1681 gagccgcgtt cccgacgaag gtcgcgaact gggcgtaaaa cggctggtca aggcccagtt
     1741 cggcgcgcag gcgggtgcga tcttcgggcg tcgcgtcctg tcccagcatc tgcgacacgg
     1801 gatcgccaac gtactggaac agcaggaatg ccaccagcgc caccgtcagc atgacaagaa
     1861 cagcctgcaa caagcggcga acgatgaacg cgagcatcct cagacactag aacgcccgac
     1921 gcaggcggcc gggctgtcaa cgccttgaaa tatagcggaa ccctgcagag acgaccgtgc
     1981 gttcgtcgca tcgtcgtcac gaaaagccga agggccgaca gcgccggccc ttcggaatca
     2041 tccgcccgat cgcctctcgg cggcgggaat cgtcgccgtc agtcgatctt tacccactcg
     2101 gccgacaggc ggttgtcggc gcgatgcacg actgtcacgt tcttgcgcat cgcccatggg
     2161 atgacctggt tgtgcaacgg gatgtgcatc acgttctggt tatgcatcat cagcgcgtcg
     2221 gcaatgatct ggttgcgctt cttctggtcg gtctcgctct tcatcgcgtc gaccaacttg
     2281 tcgaactccg aattgctgac ccggcccagg ttgaagttgc cgtcaccgtc cttgcccacc
     2341 gtgcgcgcca gcgactgaac tgaatacagc gcgtcgaagg tcggcacacc ccagccgagc
     2401 aggtaggctg acgtatcgtg cttctggatc ttcgggaagt acgtcgcgcg cggcatcgcg
     2461 ttcaggctga ccttcagccc gatctgcgcc cacatcgtga cgagcgcctt gcagatttcc
     2521 tcgtcgttga tgtagcggtt gttggggcag tcgagcgtga aagaaaaccc attcggataa
     2581 ccggccgctg ccagcagttc acgcgacttt ttctggtcgt acggcaggcg ctggtgcgct
     2641 tccggggtcc agccattgac tgcacgctgg atgatcgcgc cggtcgggtc ggacgatccc
     2701 cgcatcaccg acttctggat cgcctcgatg ttgatggcgt gatacagcgc ctgacgcacg
     2761 cgcaggtcct tgaacgggtt cttgcccttg acgtcggcga actgcagttc gtcgcgatac
     2821 tgatcgaatc cgaggaagat ggtgcggttc tccgcgccct cgacgacctt gattgacgag
     2881 ttctgcttca tgcgcgcgat atcctgcggc gcgggatcga gcaagaaatc gagttcgccc
     2941 gacagcagcg ccgccgtccg tgtcgactcc gacttgatcg gcgtatagac gacctcggtg
     3001 acgttgccct tcttcgtcgc cttgccccac caggccgggt tctcgacgaa cacggtcttg
     3061 atatcgacct cgcgcgactt gagcatgtac gggccggtgc cattggcatt gcgcaccgcg
     3121 tagctttctt ccttctgcac gaagttggtc ggtactgtga ccttgtgctt ttccgcccac
     3181 gccttgctca tgatccgcag ttccggcagc tggcgcagca gcaccggatt gggcacggtg
     3241 gtgatgatca gaaccgtgtt ctcgcccact gcgcgcgcgc tctgaattga ggtcgtgtag
     3301 gcgcggaagt tggatgtcgg gtgcagcgca cgctggatcg agaacaccac gtcgtctgcg
     3361 gtgaacggcg tgccgtcgtg gaacgtgacg ccctacgcag ctcgagccgc cagataagcg
     3421 gcgtgtccag cttccacgag acggccaaag ccggcaccag cgcgaatttt tcgtcgtact
     3481 ggagcagcgg ctcgtagacg tagctgttgg ccgtgttgtt gaggccttcg ttctgcgagt
     3541 gcggatcgaa ggtgaggatg tcgccctggc tggcccagcg caggggcttg gcctcgaggg
     3601 ccggtccggc ggccagagcg aggacggtga aggcagcaat cgcgatacga cgcatcgagt
     3661 tctcctggtg gcgccttggg gcgcttacaa ccgacggatc atcacaaaaa gcaaccgtga
     3721 cagtcaaacc ggccgggcaa tggggcaaac cctcacggcg cacccttcaa ttcgatgtcg
     3781 ccgtagtaag cgtcgatccg ctcgccggta ttgtcagtat ccgtcatgat gccgatgccg
     3841 acgaggcgac cgggcgaggc gccgaaggcc ttccgatagt cctcgacgta gttgcgttca
     3901 aactgtttcc acttcccgag ccgccggtcc ggtccgctgc ccgccacgag cagcttgacc
     3961 tgcgatgtaa aggagttcgg caggatcgtg cccaccggga aacggttctc ccagatgtac
     4021 atcaacgtcg cgtacggaag atcctgcccc gtcagcagtt tcgccgtatc gatcagcacc
     4081 tgctccttga acggcagcgt cttcttgtct ccgtcgaaga agagcatcag tcgcaccggc
     4141 gaatcttcgg catagcggtc ctggttgtcg gcactgacga tcaggtcaag gacgcgccag
     4201 cgccacgcaa cgaccggctg ctgtgccgga tcgacgtcga gcagctggcg caggccgctc
     4261 gcagccgcat cggcatgtgc atggagcacg accacaccgg tctgcggatc gcgcaccagc
     4321 cggtattccg tcggtgtctt ggtccggttg atgatccatg gccgccatcc ctgcggtaat
     4381 ccgccggggt ccgaattcgt cgagaatcgg gcgactacag tggcaggcac ctccggcgct
     4441 ggcgtggtgg cacatccggc caacatagcc agcaccggaa cgaaaacgcg cgtgaccgaa
     4501 aaacgcatgg gcgcgcgatc ctagggccag tgcgagcacg ccgcaagacg ggacgtcacg
     4561 gaggctccgt gaggcccgac tgcatcggcg cgctccacgc ctgaccgtca ttgtcgcgac
     4621 tacgctggac gcccgccatc ttggcaaacg cacgccgagg ccaggtgatc aagggtgacg
     4681 gcgctcggcc gggtttctgc acaattcccg ggatcggctg cgtcgcgcaa cgcttaccat
     4741 cgtcacgtcg aaatgaagaa tccgaacact atgaacgtgt cgctatccgc ggttgtccgt
     4801 cgttcgcgtg cgccgctgat gcgttcatcc gcgcatgact tcgcggacag cgcgcaggag
     4861 agtggggccg ccgcgacgag ccgctgggca acgcacggcg ggataggctc gccttactgc
     4921 ggaccatgac gggtttcctt gtctttgcct tcggtgcggg cgttctcggc gccggggcct
     4981 ggctggtatt aagggcgcgt cgactcgaga gtgaccgcga cttcgaacgc aaggtccatg
     5041 cacggaagaa cggctggacc tatgacgggc gccggtccgg ccgcatcgac taccgctttg
     5101 ctggaacggc gggaggcatc gattggcaga tgtggcacga cagcgaccgc ggtgacgatt
     5161 cgccgacgcc tcgggcgttc tggaccagcg cgaatctgcg aacgcccggc cttgcgctgg
     5221 tcatcatcgg gcgcaagcgg tttcaactcg aaagtggaac ggtaggtcga ctgttgatgg
     5281 gagtggtcac tggagttgcc catgcggtca ccggccacga gggccaggtc gacaaggcgg
     5341 agttctacga aagcgccatc gagatcaagg tgggccgacc ctcgttcgac gaacgactca
     5401 ccgtcgccgt cgcccccgac atgcccagga cctgggtcga cagcgaactg caggcgctgt
     5461 tgcttgagtg gcctgcaacc ggcacctccc gaatgttcaa gagtgaggac agcgtcgaag
     5521 tcacgctgcg ggcggacggc ctcaagatcg tcgtgcagaa gatgccgcag gacatcgcgc
     5581 actggcggca ccttgcgcgg ctgggcgagc acattgcacg tcagctttct cggacggagc
     5641 cgcgctgact gcgcgctttg gagagggagc atgacggacg gtcggcggca ggcgaggcaa
     5701 cttgcagtcg tcgccggcgc gagcggtctg gtcggacgcg aactggttct gcgcctgctc
     5761 cgacatcccg tttactcacg ggtcgtcgcg ctgacgcgcc ggccgctcag catagacaat
     5821 gtccgcttga gcgaagtgcc ggcccgcttc gagtcgcttg aaccgaccct gtcgagcgcg
     5881 gtgtcggaac cggcgcgact ggatgcctat tgctgcctgg gcacgacaat caggtccgcc
     5941 ggctctgagg cggcctttcg catagtcgac catgactacg tggtcgcctt cggacaatgg
     6001 gccgtccggc atcgggccca tcgtctggtg ctcgtcagcg cgctcggtgc cgatgcacgc
     6061 ggcgccgtgt tctacagccg ggtcaagggc gaaaccgaga acgccctgcg cgcacttgcc
     6121 ccgtcgtcgc tggtcatcct gcggccgagc ctgctcgacg gcgaccgcgg tgaacgccgt
     6181 ctcggagaat catttgcgct agcactggcg cggccactgc gggccgtgct gccggcggcg
     6241 tttcgtgcgg tgcgcgccga tgacgtcgcg cagtcgatga tcgatgccgc cctgctggac
     6301 ggcgcgcaag gcacgattga atccgcggca atgcacggcg ctgcggcacg tgccgactct
     6361 aggacgacag gctgatgaga tgtcgcgcgc tatgtgcgca ccgcacgtcc ggcgcattcc
     6421 agttggatcg agagtggcac tgagcgcgcc gcggcacccg tcgacactat cgcaacggca
     6481 ccagcaagag gaccggagcc gttgccggag ccggacgcat cgggaagtac cggacatcgc
     6541 gccacgccgg cagcatcgaa gcgtacagct gcgacagggg attgccgacc tgcccggtgg
     6601 attgcaccca gacggaagcc tccggcgtag caaggtcgta gatcgcccgc aggctggcgg
     6661 cgtgccgcgt gctgaagggt gcttccggac ggttggacag agcgccgaca ttgaccatga
     6721 acgtatcgcc cggatacggc gtgctcagtt cgaacaggcg cgcgagcagc gcgacgctcg
     6781 agagcggccg atgctccgcc acggcccgat gctcgtcacc ccagcgcagc ccggccacat
     6841 cgcgaccgct cgcctacgtg aggcgagtg
//
LOCUS       DATIRT010000199         2417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_519085, whole genome shotgun sequence.
ACCESSION   DATIRT010000199 DATIRT010000000
VERSION     DATIRT010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519085"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..117
                     /locus_tag="VLN25_04760"
     CDS             <1..117
                     /locus_tag="VLN25_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HSQ09913.1"
                     /translation="ARGIADAVLEGKAAALQGVVQAVTGGGEEFVEVSEGQE"
     gene            202..1089
                     /gene="tsf"
                     /locus_tag="VLN25_04765"
     CDS             202..1089
                     /gene="tsf"
                     /locus_tag="VLN25_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HSQ09914.1"
                     /translation="MAEITASMVKELREKTDAPMMECKKALSEAGGDLAKAEEILRVK
                     LGNKASKAAARVAAEGVVAVSIAGKVGAIVEVNSETDFVAKNEDFLKFAKDLGAIVGT
                     SNPADVAALSGLPLGGGTVETTRTALVGKIGENMAIRRFARIEAQGTLASYVHGGSKI
                     GVLLDISGADAELAKDVAMHIAASKPKSLDSSGVSADLLDAERRIAIEKAREAGKPEA
                     MLEKIAEGTVQKFLKEVTLLGQVFVKAEDGKQTIEQLLKARGAKVNSFVLYVVGEGIE
                     KKQNDFAAEVAAQAAAARA"
     gene            1104..1832
                     /gene="pyrH"
                     /locus_tag="VLN25_04770"
     CDS             1104..1832
                     /gene="pyrH"
                     /locus_tag="VLN25_04770"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HSQ09915.1"
                     /translation="MASKTGTPAYRRVLLKLSGEALMGDDAFGINRQTVEAMVADIGE
                     VVKLGVEVAIVIGGGNIFRGVALGAEGMDRATADYMGMMATIMNAMALQDAMRRAGIE
                     ARVQSALNIEQVVEPYIRPKALRYLEEGRVVIFAAGTGNPFFTTDTAAALRGSEVGAQ
                     IVLKATKVDGVYSADPKKDPHATRYSTITFDEALGRNLQIMDATAFALCRDQRLPIKV
                     FNITKPGALKRVVLGEDEGTLVHV"
     gene            1870..>2417
                     /gene="frr"
                     /locus_tag="VLN25_04775"
     CDS             1870..>2417
                     /gene="frr"
                     /locus_tag="VLN25_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HSQ09916.1"
                     /translation="MNVAEIQKTTEQKMLKSVDALKADLSKIRTGRAHTGLLDHIQVD
                     YYGSMVPLSQVSQVGLGDARTITVQPWEKKMIPVVEKAIRESDLGLNPATSGDIVRVP
                     MPVLTEQRRKELVKVVKHEGEAAKVAIRNLRRDANHHVKELLKAKTISEDDDRRAEET
                     VQKLTDRFVGEVDKIVSEKEKEV"
BASE COUNT          520 a          679 c          846 g          372 t
ORIGIN      
        1 gcgcgcggca ttgccgacgc cgttctcgaa ggcaaggccg cggcactgca aggagtggtg
       61 caggccgtga cggggggcgg cgaggagttt gtcgaggtca gcgaagggca ggaatagctt
      121 tgcgctgaat cgtgcggcga aaagggggcg cgagcccctt ttttgcagtg agaaagttca
      181 acgaaattgc gggagcacgg aatggcggaa atcaccgcat ccatggtgaa agaactgcgc
      241 gagaagaccg acgcgccgat gatggaatgc aaaaaggctc tgtccgaggc cggcggtgac
      301 ctggccaagg ccgaggagat cctgcgggtc aagctcggca acaaggcgag caaggcggcg
      361 gcccgcgtgg ccgccgaagg ggtcgttgcg gtaagcatcg ccgggaaggt cggtgcaatc
      421 gtcgaagtga actcggagac cgacttcgtc gccaagaacg aggacttcct caagttcgca
      481 aaggatctcg gggcaatcgt ggggaccagc aacccggccg acgttgccgc tttgtcaggc
      541 ctgccgctcg gcggcggcac ggtggaaacc acgcgcacgg ccctggtggg caagatcggc
      601 gagaacatgg cgattcgccg ctttgcgcgc atcgaggcgc aggggactct cgcaagctac
      661 gtccatggcg gcagcaagat cggcgtgctg ctggacatct ccggcgcaga cgccgaactg
      721 gccaaggacg tcgccatgca catcgcagcc agcaagccca agtcgctcga ttcgagcgga
      781 gtatcggccg acctgctcga cgccgagcgt cgtatcgcga tcgagaaggc acgcgaagcc
      841 ggcaaacccg aggcaatgct ggagaagatc gctgagggca cggtgcagaa gtttctcaag
      901 gaagtgacgc tgctcggcca ggtcttcgtc aaggccgagg acggcaagca gaccatcgag
      961 caactgctga aggccagggg tgccaaggtg aatagcttcg tgctctacgt tgtcggtgag
     1021 ggcatcgaga agaagcagaa tgatttcgcc gcggaggtcg cggcgcaagc ggccgctgcg
     1081 cgggcctgag cggggcgaaa gcgatggcat cgaagacggg cacgccggcg tatcgacgtg
     1141 tactgctgaa gctttcggga gaggccctga tgggcgacga tgccttcggc atcaaccgtc
     1201 agacggtcga ggccatggtc gctgacatcg gcgaggtggt gaagctcggt gtcgaggtcg
     1261 ccatcgtcat cggcggcggc aacatcttcc gcggggtggc gctcggcgcc gaaggcatgg
     1321 accgcgccac ggccgactac atgggcatga tggccacgat catgaacgcc atggcactgc
     1381 aggacgcgat gcggcgtgcc ggcatcgagg cgcgcgtgca gtcggcgctc aacatcgagc
     1441 aggtagtgga gccgtatatc cggcccaagg cgctgcgcta cctggaggag gggcgcgtcg
     1501 tgatttttgc ggcggggacc ggcaatccgt tcttcacgac cgatacggcc gctgcgctgc
     1561 gcggttccga ggtcggcgcg cagatcgtgc ttaaggccac caaggtcgac ggcgtgtact
     1621 cggccgaccc gaagaaggat ccgcacgcga cccggtattc gacgatcacc ttcgatgaag
     1681 ccctgggtcg caacctgcag atcatggacg ccaccgcctt cgcgttgtgc cgtgaccagc
     1741 ggctgccgat caaggtcttc aacatcacca agccgggagc gctcaagcgt gttgtcctgg
     1801 gcgaggacga aggtacgctg gtccatgttt gaccgctgag tcccgcctgt accaagcaag
     1861 gagattcgga tgaacgttgc agaaatccag aagacgacgg aacagaagat gctgaagtcg
     1921 gtcgatgcgc tgaaggccga cctgtcgaag attcgcacag gccgcgcgca taccgggctt
     1981 ctcgaccaca tccaggtcga ctactacggg tcgatggttc cgctcagcca ggtctcgcag
     2041 gtggggctcg gtgatgcgcg cacgatcacc gtgcagccgt gggaaaaaaa gatgatcccg
     2101 gtcgtggaaa aggcgattcg cgaatccgac ctcggcctca atccagcgac cagtggcgat
     2161 atcgtccggg tgccgatgcc tgttctgaca gagcaacggc gcaaggaact cgtcaaggtc
     2221 gtgaagcacg agggcgaggc ggcgaaagtg gcgatacgca atctgcggcg cgacgctaac
     2281 caccatgtca aggaactgct gaaggcgaag acgatctccg aggacgacga ccggcgagcc
     2341 gaagagacgg tgcagaagct gacggaccgt ttcgtgggag aggtcgacaa gatcgtctca
     2401 gaaaaggaaa aagaggt
//
LOCUS       DATIRT010000200         1586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_814818, whole genome shotgun sequence.
ACCESSION   DATIRT010000200 DATIRT010000000
VERSION     DATIRT010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814818"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..322)
                     /locus_tag="VLN25_04780"
     CDS             complement(134..322)
                     /locus_tag="VLN25_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HSQ09917.1"
                     /translation="MGDSLVRLTCKEAHRLVAEKLDRELPLGDRLRVRLHLFACTSCT
                     RFNRQMQFLREAMKRVGE"
     gene            complement(324..896)
                     /locus_tag="VLN25_04785"
     CDS             complement(324..896)
                     /locus_tag="VLN25_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSQ09918.1"
                     /translation="MPDAHLRDALAPLQPMLLRIARLQLRNDAWAEDVVSETLIAALE
                     GSSNFTGQSQLKTWVVGILKHKIIDHFRRGHREISLDAEVEAAGAESHDELFNADGHF
                     VTPVEPWANPEASLGQTQFLQVLQACVDKLPPALGRIFMMREWLEQDTDEICKELGIS
                     STNCFVMLYRARMRLRECLTLNWFDAKEGA"
     gene            complement(1011..>1586)
                     /locus_tag="VLN25_04790"
     CDS             complement(1011..>1586)
                     /locus_tag="VLN25_04790"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSQ09919.1"
                     /translation="ARVARELYAEARGRSLHFIDAPVSGGQAGAVNGALTVMCGGDLA
                     PFERVHPVAMAVAKAVTRIGETGAGQLSKMVNQLCIAGLVQGLSEGINFGQAAGLDMK
                     LVLQVLGKGAAQSWQMDNRGPTMIDDKFDFGFAVDWMRKDLGLCLDEARSNGAPLPVA
                     ALVDQFYADVQSMGGQRWDTSSLIRRLRKGA"
BASE COUNT          273 a          533 c          503 g          277 t
ORIGIN      
        1 gccttcagca ggggcagggt ggcgtgcaat caaacctccg atcggaaaat gcgaatcttt
       61 tcctcaacct ggaccacgtg cctgcgctgc cggcccgcat cgaagccatc ggcagctgcg
      121 tcgacgcgag gcatcattcg cccacgcgct tcatcgcctc gcgcaggaac tgcatctggc
      181 gattgaaccg ggtacagctc gtgcaggcaa acaggtgcag gcgcacgcgc agccgatcgc
      241 ccagcggcaa ttcgcggtcg agcttttccg ccaccagccg atgcgcttcc ttgcaggtca
      301 ggcggaccag cgaatcaccc aattcaggcc ccttccttcg cgtcgaacca gttcaacgtc
      361 aggcattcgc gcagccgcat gcgcgctcga tacagcatca cgaaacagtt ggtcgagctg
      421 atgccgagtt ccttacagat ttcgtcggtg tcctgctcca gccattcccg catcatgaag
      481 atccggccga gcgccggcgg cagcttgtcg acacaggctt ggagcacctg caggaactgc
      541 gtctggccga ggctggcctc cggattcgcc cacggttcca ccggggtgac gaagtggccg
      601 tcggcgttga acagttcgtc gtgggactcg gcaccggcgg cctcgacttc ggcgtcgagc
      661 gagatttcgc gatgaccgcg gcgaaagtgg tcgatgatct tgtgcttgag gatgccgacc
      721 acccaggtct tcagttgcga ttgcccggtg aagttggacg agccttcgag cgccgctatc
      781 agcgtttccg acaccacgtc ctcggcccac gcatcattgc gtagctgcaa ccgggcgatc
      841 cgcagcagca tcggctgcag cggagcgagg gcgtcgcgca ggtgggcgtc aggcataggt
      901 ggtgatctag tacagcctgc gtttcgaccc gcatggaggc gggaagtgcc cgctttccgc
      961 tgggaagtca ggggccaatg ggctcaagcg gctcaagcgg ctcaatcggc tcaagcgccc
     1021 ttgcgcaggc gccggatcag gctcgaggtg tcccagcgct ggccgcccat ggactgcacg
     1081 tcggcgtaga actggtcgac cagcgccgcg accggcaacg gtgctccgtt gctgcgtgct
     1141 tcgtcgaggc acagtccgag atccttgcgc atccaatcca cggcgaagcc gaagtcgaac
     1201 ttgtcgtcga tcattgtcgg cccgcggttg tccatctgcc agctctgcgc ggcccccttg
     1261 cccaacacct gaagcacgag cttcatgtcg aggccggcgg cctggccgaa gttgatgcct
     1321 tcggacaatc cctggaccag cccggcaatg cacaactggt taaccatctt cgacagctgc
     1381 ccggcgccgg tttcgccgat gcgggtcacg gccttcgcga cggccatcgc caccgggtgc
     1441 acgcgctcga acggcgcgag gtcgccgccg cacatcacgg tcagcgcacc gttgacggcg
     1501 ccggcctggc cgcccgacac cggggcgtcg atgaagtgca ggctgcgacc gcgggcctcg
     1561 gcatacagct cgcgcgccac gcgggc
//
LOCUS       DATIRT010000201         4391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_814996, whole genome shotgun sequence.
ACCESSION   DATIRT010000201 DATIRT010000000
VERSION     DATIRT010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814996"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VLN25_04795"
     CDS             <1..537
                     /locus_tag="VLN25_04795"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HSQ09920.1"
                     /translation="GSPLEIDYPTLSGSMSLRVDDGRFMQVDNRGAGRLLTLLSLQSL
                     SRTLMTNARESFGEGFAFSVIKADATVTRGVIATENFSMTGAGAAALISGSVNLANET
                     QQLHLVVLPEIDASTAALAVGIANPIIGLGALLANMVLKAPLSRAFALEYDITGTIGD
                     PVITRRGRVAANPSEPVR"
     gene            534..1373
                     /locus_tag="VLN25_04800"
     CDS             534..1373
                     /locus_tag="VLN25_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HSQ09921.1"
                     /translation="MTLSPPLPGTVRVAAVQMVSTTQVDTNLATAAQLIAEAAATGAR
                     LISLPEYFCMMGRQDTDKVAVREADGRGPIQDFLASQARQHGVWLVGGTLPLEAPAPD
                     RVFNTTLVFDPSGARVARYDKVHLFRFSRGTESYDEAVTIAPGSRVSTFGLQPDAGPE
                     LKVGLSVCYDLRFPELYRAMNQPDLILVPAAFTATTGRAHWETLLRARAIENLCYVLA
                     AAQGGRHENGRATFGHSMLIDPWGEVIAHRPDGAGVVFGDIDPQVIARCRASLPALTH
                     RVM"
     gene            1468..2928
                     /gene="tldD"
                     /locus_tag="VLN25_04805"
                     /pseudo
     CDS             1468..2928
                     /gene="tldD"
                     /locus_tag="VLN25_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747615.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
     gene            3138..3719
                     /locus_tag="VLN25_04810"
     CDS             3138..3719
                     /locus_tag="VLN25_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HSQ09922.1"
                     /translation="MWLTRLLVGAYPRWIGSAPKATQRIYFGNHASHMDTVVLWAALP
                     EELRSNTRPVAAKDYWDRPGIRGAIARNELNAVMVARSREDPNADPLEPLRDALEHGF
                     SLIIFPEGTRSAQSLPGPFKSGLFHLAGDFPQVELIPVYIENLHRSMPKGAFVPVPII
                     CTVRFGAALARIDGEDKEAFLERARAAVIRLAS"
     gene            3736..>4391
                     /locus_tag="VLN25_04815"
     CDS             3736..>4391
                     /locus_tag="VLN25_04815"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HSQ09923.1"
                     /translation="MTLTLNETFLAVFCSLAILSAVATGIGQWATRQTFDATRLARLG
                     VLNSRIRSSWSVLAVFTIAFALGPVALLVVFALASFFALREFVSLTPIKSSDHWALVV
                     AFYLVIPAQYLLLGFGRASVFSTFIPVYIFLLLPVIVAIRQDIERYLERVAKVQWGLM
                     ISVYCISHAPAIATLPIKGNEGRGPLLLLFFLLVLFLTDVLQIVFSAALNGRALKSNP
                     "
BASE COUNT          732 a         1469 c         1372 g          818 t
ORIGIN      
        1 ggttcaccgc tcgaaatcga ttacccgacc ctgtcgggtt cgatgagcct gcgcgtcgac
       61 gatggacgct tcatgcaggt cgacaaccgc ggcgccggcc gcctgctcac gctgctgtcc
      121 ctgcagtcac tgtcgcgcac gctgatgacc aatgcccgcg agtcgttcgg cgaaggcttc
      181 gccttcagcg tgatcaaggc ggacgccacg gtgacgcgcg gtgtgatcgc caccgagaat
      241 ttcagcatga ccggcgccgg cgcggcggcg ctgatctcgg gcagcgtcaa tctggcgaat
      301 gaaacccagc aactgcacct ggtggttctg cccgagatcg atgcctcgac cgctgcgctg
      361 gccgtcggca tcgccaatcc gatcatcgga ctcggggcct tgcttgccaa catggtgctc
      421 aaggcaccac tgtcgcgtgc cttcgcgctc gagtacgaca tcaccggcac cattggggac
      481 cccgtgatca cccgccgcgg gcgcgtcgcg gccaacccgt cggagcccgt gcgatgaccc
      541 tctccccgcc tttgcccgga acggtgcgcg ttgccgctgt ccagatggtg tccaccacgc
      601 aggtcgacac aaatctcgcg acggccgcac agctgatcgc cgaggccgcc gcgaccgggg
      661 cacgactaat ctcactgccg gagtatttct gcatgatggg ccggcaggac accgacaagg
      721 tcgcagtgcg cgaagcggac ggacgcggac cgatccagga tttccttgca tcgcaggcgc
      781 gacaacacgg tgtctggctg gtgggcggaa cgctgccgct cgaggcgccg gcacccgacc
      841 gcgtgttcaa cacgacactg gtattcgatc cgagcggcgc tcgggtagct cggtacgaca
      901 aggtgcatct gttccgcttt tcccgcggga ctgaaagcta cgacgaggcc gtgacgatcg
      961 cgccgggctc gcgcgtgtcc acattcggtc ttcagcccga tgccggaccg gaattgaagg
     1021 tcggactgtc cgtgtgctac gacctgcgtt ttccggagct gtatcgcgcg atgaatcagc
     1081 ccgacctgat cctcgttccc gcggcgttca ccgcaacgac agggcgcgcg cactgggaga
     1141 cactgctgcg cgcccgtgcc atcgagaacc tctgctacgt acttgcggcc gcgcagggcg
     1201 ggcgccacga gaacggccgc gccacttttg gccactccat gctgatcgac ccgtggggcg
     1261 aggtgattgc ccatcgccct gatggcgccg gggttgtgtt tggcgatatc gaccctcagg
     1321 tcattgcccg ttgtcgggcc tcattgccag cactgacgca tcgagtgatg taaggcctgt
     1381 gtcaggccgc gctcggcccc ttccggccgg cccagcccta tagacttccg gccacgatcc
     1441 cttccacttt cctgactgcg caactgaatg aatctttccg atcctgcgct cgaccgcctc
     1501 gcaacggcca aggcgttgct gctcgacccc tacgacctcg atgaaggccg cctgcaacag
     1561 gtattcgcga ccatcttcga gcatcgcgcc gactacgccg atctttattt ccagtacaca
     1621 cgcaacgagt cgttctcgct cgaagagggc atcgtcaaga gcgggagctt cgacatcgat
     1681 cagggcgtcg gcgtccgggc ggtcgtgggc gacaaaacgg ccttcgcgta ttccgacgac
     1741 atttcgcgcg agtcgctgaa cgaggcggca gtgtcagcgc gtgccatcgc gcgtcgcggc
     1801 agcggccgcg tccgcgttgc gccccgcgtg acgtcgcgct cgcaagtggc gttctatggc
     1861 accgatgatc cggtcgcatc gctcgatgct gtcgagaaaa tccggctgct cgaacgcgtc
     1921 gagcatctcg cccgtgcgcg cgatccgcgc gtcaagcagg tgatggccaa tgtcggcgcc
     1981 gagtacgacg tcattctcgt ggcgcgcagc gacggcgtgc tcgccgcgga tgtgcggccc
     2041 ctggtgcaac tttcggtgac cgtgatcgtc taggaaagcg gccgccgtga gcaaggattt
     2101 tccggcggcg gcggacgaca cgacctcgcc tacttttcgg aagagcgcct ggcagaacac
     2161 gcgcacaagg ccgttgacca ggccctcgtc aatctcgacg cccgcccggc cccggccggc
     2221 gtgatgcctg tcgtgctcgg gcccggctgg cctgggatcc tcctgcacga agccgtcggc
     2281 catggcctcg agggtgactt caaccgcaag gggtcgagcg cgtttgccgg ccgcgtcggc
     2341 gaacgcgtgg cttcgcgcgg cgtcacggtc gtcgacgacg gcacgctgtc gaatcgccgg
     2401 ggatcgctga acgtcgacga cgagggcaat gccacccagc gtacggtcct gatcgaggac
     2461 ggcatcctgc gcggctatat ccaggactct ctcaatgcgc gactgatgaa ggtcgcaccc
     2521 acgggcaatg gccggcgcga gtccttcgcc catctgccga tgccgcgcat gacaaacacc
     2581 tacatgctcg gcggcgtgga cgatccgcag gaaatcatcc gctcggtcaa gaagggcctt
     2641 tatgcgacca acttccgcgg gggccaggtc gatatcacca atggcaagtt cgtgttcgcc
     2701 atggcggagg cctatctgat cgaaaatggc cgcgtcaccg cgcccgtcaa gggagccacg
     2761 ctgatcggca acggccccga cgcattgacc cgcgtgacga tgatcggcaa cgacatggaa
     2821 ctcgatctgg gcatcggcac ctgcggcaag gacgggcaga gtgtgccggt cggcgtcggc
     2881 cagccaacgc ttcgcatcga aggactgacc gtcggcggaa cgggataacg tcgcacacgt
     2941 ctcgcacgcg atctgcactt tcacctgcac tcgcacctgc attcgcagcg aactgacgct
     3001 tcgcgcataa cgacgtcacg tcgacccggg ctgcatcgca agcggcacca gcgctcagcg
     3061 acttacctgc gcttggcata attcgcacac aagaacagaa attcccggga ggcccgctga
     3121 agttcctgcc gccgatcgtg tggctgacac gcttgctcgt cggcgcgtat ccgcgctgga
     3181 tcggctcggc gcccaaggca acacagcgca tctatttcgg caatcacgcg agtcacatgg
     3241 acacggtggt gctgtgggca gcgctgccgg aggaactgcg ttcgaatacc cggcccgtgg
     3301 ccgccaagga ctattgggac cggccgggca tacgcggggc cattgcgcgc aacgaattga
     3361 acgccgtgat ggtcgcccgc agccgcgaag acccgaacgc cgatccgctc gagccgctgc
     3421 gcgatgccct cgagcacggc ttttcactga tcatttttcc cgaaggcaca cgcagtgcgc
     3481 agtcgctgcc cggcccgttc aagagcgggc tgtttcacct tgccggcgac ttcccgcagg
     3541 tcgagctgat tccggtgtac atcgagaatc tccatcgcag catgcccaag ggcgcgttcg
     3601 tgccggtgcc gatcatctgc acggtccgct ttggcgccgc gctggcgcgc atcgacggtg
     3661 aggacaagga ggcgttcctc gagcgcgccc gcgcagccgt gatccggctg gccagctgag
     3721 cactgttcga cacctatgac cctcacgctc aacgaaactt tccttgcagt gttctgttcg
     3781 ctggcgattc tgtcggcggt ggccacagga attggccagt gggcgacgcg gcagacgttt
     3841 gacgcgacac gcctggcccg cctcggggtg ctgaactcgc gcatccgcag ttcgtggtcc
     3901 gtgctggcgg tgttcacgat cgccttcgcg ctcggccccg ttgcgctgct ggtcgtgttc
     3961 gcgctggcgt cgttctttgc gttgcgcgag ttcgtgtccc tgacgcccat caaatcatcc
     4021 gaccattggg cgctcgtcgt cgccttctac ctggtgattc ccgcgcagta cctgctgctg
     4081 ggttttggcc gcgccagtgt gttctccacc ttcatacccg tgtacatctt cctgctgttg
     4141 ccagtcatcg tcgcgattcg ccaggacatc gagcgctatc tggagcgtgt cgccaaagtg
     4201 caatggggac tgatgatcag tgtctactgc atcagccacg cgcccgcgat cgcgaccttg
     4261 ccgatcaagg ggaacgaagg ccgcggtccc ctgctgctgc tgttcttcct gctggtgctg
     4321 ttcctgaccg acgtgttgca gatcgtgttc agtgcggccc tcaatggacg tgccttgaag
     4381 tcgaacccca a
//
LOCUS       DATIRT010000202         5317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1700322, whole genome shotgun sequence.
ACCESSION   DATIRT010000202 DATIRT010000000
VERSION     DATIRT010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1700322"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VLN25_04820"
     CDS             <1..471
                     /locus_tag="VLN25_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSQ09924.1"
                     /translation="WRIGFAEDACCFVIVPADLKSLLRQLARHARVTLRALRDLPGEL
                     IRRRTTLLFVAWHLLFPWTDLAFTLAFVPGVVLSLAYSSHLLLGPMALALLPAALLLN
                     IVIFYVMRAMFHDHGLKIRRNVGGLVVYLFGYSLLLQPARVAGYLAGLLRRRPA"
     gene            complement(532..4485)
                     /locus_tag="VLN25_04825"
     CDS             complement(532..4485)
                     /locus_tag="VLN25_04825"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HSQ09925.1"
                     /translation="MEPAPAPVPPPSLPRPSWLGSLAAVLGPGLAAILLLSGVAWWMA
                     TTAPGLRLAATLVNAIVPGITLKGVDGSLSGGLRAASFVYTRESWSLSIEDLVLEPRA
                     WTLWDRRLDVERVSARRVHIDWVSDDGPGTVPASLGLPFDLVVRQGNIQELALGARGA
                     PAMAFRRIELAATMGPTGLDVARITAEVDRTRIAARGSIGALLPFPTQAYARLETSAQ
                     ERTVVADIEARGSLQELTLELRADDDAARAKGTAILRVFDDVPLGRLQAEVEAFDPAL
                     WFNDVPTMQLRARAELLPTTRADGRWSVSGPFSVENSMAGPVNKNRAPLRSLRGSLDW
                     GNDTLKLDIARAEGVRGTAHGTLTWSTAGGLAVQAQFNGIDAATLHSGAVTTNATGQV
                     TYTFSKGVQRFNGSARNASGLPLSADVVASIRDQVLELSTARLQLGAGRADLRGRLEF
                     GGAQALQLSGNFAELDLSQLVRGIDTRLNGSLEVQGRLQAPFSGQGRFALADSRIAGR
                     PITGRGSMSIADRRFDADVELRSGDARLTALGGLGGGREVQVELVAPEIEPLLPGYAG
                     RLDLQATIAGEIEALRIRATAAATNLRVPGGHQIASVVTSFNGGVSANEPLALTVKLS
                     GHTSPGGPESSLAGATLVGRGTTTNATFELNGATATQQPIRIIASGGVSDGAWRGSLV
                     AAEAGAPLDLLIRAPAPLMISLDAIALGPMDFEMRGARFMAVEIRSADGRWRTSGTFE
                     GMQPQALDAEARAPRRVVRSGAGDRVPLTLAGRWNLEYSDTVNGIAVIERTGGDIYSG
                     IDALNPIGVSDVGAALSVLDDRVTGNVYVRGRALGKIDAEIDAYVDPSAPGGRLLAQN
                     RPFRVVIDSTLPDLSWIGPLIGDNVQFGGKASISAAIGGTPADPTSTGALRGEALRLA
                     WVDQAVRLENGKLDAVLDDGVLVINELLFSGNPRVAPLDKRALEGLDSNRPFEVRAVG
                     RIALRSLAGSIGVQATQLPVLQRADRWMVVSGSGGITLSPQKADLYAKLTVDGAYINF
                     DNLRANRGLPNDVVVTRAGAQRKATTTAPVDVSLAMQGNLGQRFYIEGAGLETRLAGE
                     VNIFGRPSQLRAEGSVRTVDGVFAGYGQRLQIERGIVTFLGPIDNPALNVLAVRSGLP
                     VEVGVAIGGTAQRPFVRLHSDPAMSDTEKLNWLVLGRPPGASDGNDRAMLSAAASALF
                     SGQADSASAGLIRSLGIDQITLQPGQSSGSLLPRETVAGRLRSSGVSTPNAAAADFLA
                     VGKRINDDLFLSFEQALSGAEYFVALNYRLTRQLSLIARAGSTNSLDLVYSIAFD"
     gene            complement(4491..>5317)
                     /locus_tag="VLN25_04830"
     CDS             complement(4491..>5317)
                     /locus_tag="VLN25_04830"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HSQ09926.1"
                     /translation="QTGEFAYADLIFPPQPGGARDSVGVLVEISDIENLQINRWGIGA
                     ARSKLMGPRDGRNVETQLALNFEHERRETPLDPPITLDVLSTTYTWTRRDVDDITHPR
                     KGNLIQLQGSVGIGGFSADDAFVRGFGSLVQYLPIGARDVLILRGQLGAVEAESTNVV
                     PNKFLFRTGGALTVRGYDFESIGVEQGGAIVGGRALAVASVEYVKWLERWGGDWGVAA
                     FVDAGDAAGRFRDLDPALGYGLGVRWRTPAGPLAVDVAWGERFQQLRVHFSVAIAF"
BASE COUNT          879 a         1789 c         1738 g          911 t
ORIGIN      
        1 tggcgcatcg gttttgccga ggacgcgtgc tgcttcgtca tcgttccggc cgatctcaag
       61 tcgctgctgc gccaactcgc gcgccatgcg cgcgtgacgc tgcgcgcgct gcgcgacctg
      121 ccgggtgaac tgatccgtcg acgcacgacg ctgctgttcg tcgcctggca cctgctgttt
      181 ccgtggacgg atcttgcatt cacgctcgcc tttgttccgg gcgtggtcct gtccttggcg
      241 tacagcagtc acctgctgct gggaccaatg gccctggcgt tgctgcccgc cgctttgctg
      301 ctcaacatcg tgatcttcta cgtgatgcgc gcgatgttcc atgatcacgg cctgaagatc
      361 cggcgcaacg tcggcggcct ggtggtgtac ctgttcggat acagcctgct gctgcagccg
      421 gcgcgcgtgg ccggctacct ggcgggcctc ttgaggcgac gtcccgccta gccattcaag
      481 tgcgcgcgcc agccgcgccc gcgaccgccc accggcgcga ggtcgatacg ttcaatcgaa
      541 cgcgatcgag taaaccagat caagcgagtt ggtactgccg gcacgggcga tcagagacag
      601 ctggcgcgtc aggcggtagt tcagcgctac gaagtactcg gccccgctga gcgcctgctc
      661 gaacgagagg aatagatcgt cattgattcg tttgccgacc gcgaggaagt cggcggcggc
      721 cgcattcggc gtcgagacgc cgctgctgcg cagccggccg gcgaccgtct cgcgcggcag
      781 cagtgaaccg ctgctttgcc cgggctgcag cgtgatctgg tcgatgccga ggctgcgtat
      841 cagtccggca ctggcgctgt cggcctgccc agagaacaag gcgctcgcgg cggccgacag
      901 catcgcccga tcgttgccgt cactggcgcc cggcggacga ccgagcacaa gccagttgag
      961 cttctcggtg tccgacatcg caggatcgga gtgcaggcgg acgaagggac gctgcgccgt
     1021 tccgccgatg gcgactccaa cctcgaccgg cagacccgag cgcaccgcca gcacgttgag
     1081 tgccggattg tcgatcgggc caaggaaggt gacgatgccc cgctcgatct gcaggcgctg
     1141 tccatagccg gcaaagacac catcgacggt tcgcacgctg ccttccgcgc gcagttggga
     1201 cggccttccg aagatgttga cctcgcccgc caggcgcgtt tcgaggccgg cgccttcgat
     1261 gtagaagcgt tgtccgagat tgccttgcat tgcgagagac acgtcgacag gtgctgtcgt
     1321 cgtggccttg cgttgtgcac cagcgcgcgt gaccacgacg tcgttcggca ggccgcgatt
     1381 ggcgcgcagg ttgtcgaagt tgatgtaggc gccgtcgacc gtcagtttgg cgtacaggtc
     1441 cgctttctgc ggagacaagg tgatgccgcc gctgcctgac acgaccatcc accggtccgc
     1501 gcgctgcagt accggcaact gcgtggcctg tactccgata ctgccggcga gcgaccgcaa
     1561 tgcgatgcgg cccaccgccc gcacctcgaa cggtcggttg gagtcgaggc cctcgagcgc
     1621 gcgcttgtcg agcggggcaa cgcgcgggtt gccggaaaac agcagttcgt tgatcacgag
     1681 tacgccgtcg tccagcaccg catccaactt tccgttctcc agccttaccg cctggtcgac
     1741 ccaggcaaga cgaagcgctt cgccgcgcag tgcaccggtt gatgtcgggt ccgccggggt
     1801 cccgccgatg gctgcgctga tcgatgcctt gccaccgaac tggacgttgt cgccgatcag
     1861 cgggccgatc cagctgaggt cgggcagggt cgaatcgatc accacgcgaa atggccgatt
     1921 ctgcgcaagc agccggccac cgggcgcgga agggtcgaca taggcatcga tttcagcatc
     1981 gatcttgccg agcgcgcgtc cacggacgta gacgttgccg gtgacccggt cgtcgagcac
     2041 gctgagtgca gcgccgacgt cgctgacacc gatcgggttg agtgcgtcga tgccgctgta
     2101 gatgtcgcca ccggtgcgct cgatgaccgc gatgccattg acggtgtcgc tgtactcgag
     2161 gttccagcgg ccggcgagtg tcagcggaac ccggtcgccg gcgccgctgc gcacaacgcg
     2221 gcgcggggcc cgcgcctcag cgtcaagggc ttgcggctgc attccttcga aggtgcctga
     2281 cgttcgccag cgcccgtccg cactcctgat ctcgacggcc atgaagcgcg cgccccgcat
     2341 ctcgaaatcc atgggaccga gggcgatcgc atcgagcgag atcatcaacg gggcaggtgc
     2401 gcgaataagc agatccagcg gcgcgcctgc ttccgcggcg accagcgatc cgcgccaagc
     2461 gccgtcgctc acgccaccgc tggcgatgat ccggattggc tgctgcgtag cggtggcacc
     2521 gttgagttca aacgtcgcgt tggtggtcgt gccgcgtccg accagcgtgg cgccggccag
     2581 tgagctttca ggcccgccgg ggctggtgtg tccactcagt ttcacggtta atgccaacgg
     2641 ttcgttggcc gacacaccac cgttgaacga cgtgaccacg ctggcaatct gatgtccgcc
     2701 cggtacgcgc aggttcgtcg ccgccgccgt ggctcttatg cggagggcct cgatctcgcc
     2761 cgcaatcgtc gcctggaggt cgagccgtcc tgcgtagccc ggcaacagcg gctcgatctc
     2821 gggcgctacc agttcgacct gaacttcgcg cccgccgccc agcccgccga gtgcagtcag
     2881 acgtgcatcg ccggagcgca attcaacgtc ggcgtcgaac cggcggtcgg cgatactcat
     2941 ggatccgcgg ccggtgatgg gccgaccggc gatgcggcta tcggcgagtg cgaatcggcc
     3001 ctgcccgctg aacggtgcct gcaggcgtcc ctgcacttcg aggctaccgt tcaggcgcgt
     3061 gtcgatgccg cgcacgagtt gcgagaggtc gagttccgca aagttgccgc tgagttgcag
     3121 cgcctgcgcg ccgccgaatt caagtcggcc acgtagatcc gcgcgtccgg caccgagctg
     3181 cagtcgagcg gtcgacaatt ccagcacctg atctcgtatc gatgcgacca cgtcggcgct
     3241 gagcggcagt ccactcgcat tgcgcgcact gccgttgaaa cgctgtacac ccttgctgaa
     3301 cgtatacgtc acctggccgg tcgcgttcgt ggtgacggcg ccgctgtgca gcgtggcggc
     3361 gtctatgccg ttgaactgcg cctggaccgc cagaccgccc gctgtggacc acgtcagtgt
     3421 cccgtgtgcc gtgccgcgca cgccctcggc gcgggcgatg tcaagcttga gcgtgtcgtt
     3481 gccccagtcg agcgatcctc gcaacgatcg gagtggcgcc cggttcttgt ttaccggccc
     3541 cgccatgctg ttctcgactg aaaaaggtcc ggacaccgac catctgccat cggcacgggt
     3601 cgtcggaagc aactcggcac gggcgcgcag ctgcatcgtc ggcacatcgt tgaaccacag
     3661 cgcggggtcg aacgcttcca cctcggcctg caggcgtccg agtggcacgt cgtcgaacac
     3721 ccgcaggatt gctgtgcctt tggcgcgggc tgcatcgtcg tccgcgcgca gttcgagcgt
     3781 cagctcctgt agcgaaccac gcgcctcgat atcggcaacc acggtgcgtt cctgggcact
     3841 ggtttcaaga cgagcgtagg cctgcgtcgg aaacggcaac agggcgccga tacttcctcg
     3901 tgcagcaatg cgggtgcggt cgacttcggc ggtgatgcgg gcgacgtcga gacccgtcgg
     3961 ccccattgtt gctgccagct cgatgcgtcg aaacgccatg gcaggcgcgc cgcgcgcccc
     4021 gagcgcgagt tcctggatgt tgccctggcg cacgacgaga tcgaacggca ggcccagcga
     4081 tgcaggcacc gtgcccggcc cgtcatctga tacccagtca atatggacgc gacgggctga
     4141 cactctctcg acgtcgaggc gccggtccca cagtgtccat gctcgcggtt cgagcaccag
     4201 gtcctcgata ctcagggacc atgactcgcg cgtgtagacg aacgaggctg cgcgcaatcc
     4261 gcccgagagc gatccatcga cacccttgag cgtgatgccc ggaacaatgg cgttgaccag
     4321 cgtcgcagcc agtcgcagac cgggcgccgt cgtcgccatc caccacgcga cgcccgaaag
     4381 cagaaggatt gccgccagtc cgggcccgag cacggcagcg agcgagccga gccatgacgg
     4441 gcgcggaagc gagggcggag gaacgggggc gggcgcgggc tccatggaac tcagaacgca
     4501 attgccaccg agaagtgcac acgcaactgc tggaatcgtt caccccaggc cacgtcgacc
     4561 gccagcggtc ccgccggagt gcgccagcga acacccagtc cgtagcccag cgcggggtcc
     4621 agatcgcgaa agcgtcctgc ggcgtcaccg gcatcgacga acgccgcgac cccccaatcg
     4681 ccgccccagc gctccagcca cttgacatac tcgactgagg cgaccgccag tgcccgacct
     4741 ccgacgatgg caccaccctg ctcgacaccg atgctctcga agtcatagcc gcgcacagtc
     4801 agcgcaccac cggtacggaa caggaacttg tttggcacga cgttcgtgct ttcggcctca
     4861 accgcgccca gctgaccgcg caggatcagc acgtcgcgcg cgccgatggg gagatactga
     4921 acgagactgc cgaacccacg cacgaatgca tcgtcggcag agaacccgcc gatacccacc
     4981 gacccctgca gctggatcag gtttcctttt ctcggatggg tgatgtcgtc gacatcgcgg
     5041 cgggtccagg tataggtggt cgatagcacg tcgagcgtga tgggcggatc gagcggtgtc
     5101 tcgcggcgtt cgtgctcgaa gttcagggcc agctgtgtct cgacattgcg gccgtcacgc
     5161 ggtcccatca gcttcgagcg cgccgcgcca atgccccagc ggttgatctg caggttttcg
     5221 atgtccgaga tttcaaccaa cacgccgacg ctgtcgcgag caccgcccgg ctgcggcggg
     5281 aagatcaggt cggcataggc gaactcaccg gtctggt
//
LOCUS       DATIRT010000203         1618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1306756, whole genome shotgun sequence.
ACCESSION   DATIRT010000203 DATIRT010000000
VERSION     DATIRT010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306756"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..1467)
                     /locus_tag="VLN25_04835"
     CDS             complement(268..1467)
                     /locus_tag="VLN25_04835"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HSQ09927.1"
                     /translation="MARQVQDAYIVAATRTPIGKAPKGMFRNTRPDDLLVAAIRSAMK
                     QVPSLDPAAVEDAIIGCAMPEAEQGLNMARIAVLLAGLPNTTGGVTINRFCSSGLNAV
                     AMAADRIRVGEADVMIAGGAESMSMVPMTGNKPAFNAQIFEKDENVGIAYGMGMTAEK
                     VAAQWKVTREMQDAFAVESHQKAIAAQKAGEFSDEMTPIEVIEKFPNLVSHELEVKTR
                     SAGLDEGPRADTNVEALAKLRPVFAAKGSVTAGNSSQTSDGAGALIVVSERALKQFNL
                     TPLARFVSFAVKGVPPEIMGIGPKYAIPAALAHAGLKAEDMGWIELNEAFAAQAVAVI
                     NDLGLDRSKVNPMGGAIALGHPLGATGAIRAATVVHALRRRNLKYGMVTMCVGTGMGA
                     AGIFERV"
BASE COUNT          256 a          568 c          488 g          306 t
ORIGIN      
        1 ggacgatgag cacgcctgcg tggcagcgct gcgagtcgcc gaaagtcgtc gcctgcagtt
       61 cgaagtcgtc gtccgtcgtg accgtcgcct tatgcatcgt cgcccagaac aacgctcctc
      121 accgaacttc gctatccacg acccgtgcgg cgcagagcgg cgcccgccgc accgcgcgca
      181 ctcacggtgg ccagcggagc gcagcgcagt cggctacctg cttcgacttc gtctggctct
      241 gccagcgaag tcgaacgaaa taatccgcta caccctctcg aatatccctg ccgcccccat
      301 ccccgtgccg acgcacatcg tcaccatgcc gtacttgagg ttgcgacgac gcagcgcatg
      361 cacgacggtg gcggcacgga tcgcgccggt cgctcccaac ggatggccca gcgcgatcgc
      421 accgcccatc gggttcacct tgctgcggtc gagcccgaga tcgttgatca cggccaccgc
      481 ctgcgcggcg aacgcctcgt tcagctcgat ccagcccatg tcctcggcct tgagtcccgc
      541 gtgagcaagc gcggccggta tcgcgtactt ggggccgatg cccatgatct cgggcggcac
      601 gcccttgact gcgaaactga cgaagcgcgc cagcggcgtg aggttgaact gcttgagcgc
      661 ccgctcggac acgacgatca gtgcaccggc cccgtccgac gtctgcgaac tgttgcccgc
      721 ggtgacgctg cccttggcgg cgaacaccgg ccgcagtttt gccagcgcct cgacgttcgt
      781 gtccgcgcgc ggaccctcgt ccaggccggc cgagcgggtc ttcacctcga gttcgtggct
      841 caccaggttc gggaacttct cgatcacttc gatcggcgtc atctcgtcag aaaactctcc
      901 agccttctgc gcggcgatcg ccttctggtg tgattcgacc gcgaacgcat cctgcatctc
      961 tcgcgtgacc ttccactggg cggcgacctt ttcggcggtc atgcccatgc cgtaggcgat
     1021 accgacgttc tcgtccttct cgaagatttg tgcattgaag gccggcttgt tgcccgtcat
     1081 cggcaccatg ctcatcgact cggcaccgcc ggcgatcatg acgtcggcct cccccacgcg
     1141 aatgcggtcg gcggccatgg ccacggcatt cagccctgac gagcagaagc gattgatggt
     1201 cacgcccccg gtggtgttcg gcagaccggc cagcagcacg gcaatgcgcg ccatgttgag
     1261 gccctgctcg gcctccggca tcgcacagcc gatgatcgcg tcctcgaccg ctgccgggtc
     1321 gaggctcggc acctgcttca tcgccgagcg gatggccgcg accagcaggt catccggccg
     1381 cgtgttgcgg aacatgccct tcggggcctt gccgatcggc gtgcgcgtcg cggcgacgat
     1441 gtaggcatct tgaacttgac gggccatctt gaactccaga ctttgattcg gttgccgggg
     1501 agatccaaga ggggctggca agcccctctt gcgtccagat aaattggatg tcgcagggaa
     1561 accggtcagt tcctgaccgg tttccctgtc gacatcattc ccatcatgcg ttcctgcg
//
LOCUS       DATIRT010000204         2999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_913525, whole genome shotgun sequence.
ACCESSION   DATIRT010000204 DATIRT010000000
VERSION     DATIRT010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913525"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="VLN25_04840"
     CDS             complement(<1..860)
                     /locus_tag="VLN25_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HSQ09928.1"
                     /translation="MNAPASHAPLPASLATALAARFGDRYSVAAAVREHHGRDESPYA
                     PLPPDAVVFAESTDDVAAVVQLAAAHRVPVIPYGVGSSIEGHLLALHGGISIDLGRMN
                     AVLAINGEDLTVTAQAGITRKQLNVAIRDTGLFFPIDPGADATIGGMAATRASGTNAV
                     RYGTMRENVLALTVVTAEGKVIRTASRAKKSSAGYDLTRIFVGSEGTLGVITEVTLKL
                     YPQPEAISAATCSFPSVDAAVQTTIATIQLGVPIARCELLDALTVKAVNQRDKLGLLE
                     LPLLLFEFHGS"
     gene            929..1495
                     /locus_tag="VLN25_04845"
     CDS             929..1495
                     /locus_tag="VLN25_04845"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HSQ09929.1"
                     /translation="MGKRLTKIATRTGDDGTTGLGDGTRTRKDALRVAAMGDVDELNS
                     TLGVLLTEPMPDDVRADLVDIQHDLFDLGGELCIPGYTMIREAQIERLDQRLEHYNAS
                     LSRLAEFILPGGSRAAALAHVSRTVCRRAERSVVALGAAEPVNGAPRQYLNRLSDLLF
                     VLARVLNRHAGGSDVLWQPRKNAASPDA"
     gene            1710..>2999
                     /locus_tag="VLN25_04850"
     CDS             1710..>2999
                     /locus_tag="VLN25_04850"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HSQ09930.1"
                     /translation="MKFTTRNALAAILLGAFTAPFAPVAAAEPVRPLIPVFDAATISA
                     RCDGELGAVRKQLKATEARKGAGGIFAELNALTIHFADFAYPVYLLQNVAPDKATRDA
                     AQACLEKLLPFETEVYQSVALYQRVQAVKPRDLTETVYQQDLFEKFEDGGATLPPDKR
                     KRAKEIIDELERMSLTFQKNINDDATTVIITPAEADGMPEAWKAARKRDDSGNFVLGM
                     DYPTYVPFLELATNADARKRVWMAKSREGGEKNLKILEQALQLRYELAQLHGQPDFAS
                     YALKRRMAQTPAAVFDFLGKVKAAADPLQEKEFGELRAEKARLDGTPLADTKLNRWDV
                     NFLKERVRKARYSVDQEALRAYFPTEASVQYAMRLADRLYSVEFVARDVPRWHSDVRY
                     YDVYDRLPDGKRGAFVGGIYLDLFPRDGKYNHAAAFPV"
BASE COUNT          544 a          957 c         1015 g          483 t
ORIGIN      
        1 gagccgtgga actcgaaaag aagaagcggc aattcgagca ggcccagctt gtcgcgctga
       61 ttcacggcct tcactgtcag ggcgtccaac aactcgcagc gcgcgatcgg cactccgagc
      121 tggatcgtcg cgatggtcgt ctgcactgcg gcatcgacac tcggaaacga gcaggtcgcg
      181 gccgagatcg cttcgggctg cgggtacagc ttcagcgtca cctcggtgat cacgccgagc
      241 gtgccttcgg agccgacgaa gatgcgagtc aggtcatagc cggccgagct ctttttcgcg
      301 cgacttgcgg tgcgaatgac cttgccttcc gcggtaacga ccgtgagtgc caacacgttc
      361 tcgcgcatcg tgccgtagcg gaccgcattg gtgccggagg cgcgcgtggc cgccatgccg
      421 ccgatggtcg catccgcgcc cgggtcgatc gggaagaaca ggcccgtgtc cctgatcgcc
      481 acattgagtt gcttgcgtgt gatgccagcc tgcgcggtga cagtcaggtc ctcgccattg
      541 atcgcgagca cggcgttcat tcggcccagg tcgatcgaga tgccgccgtg cagggcgagc
      601 agatgccctt cgatcgacga tccgacgcca tagggaatga ccggcacccg atgcgcggcc
      661 gcgagctgca cgacggcggc aacgtcgtcg gtggattcgg cgaagaccac ggcgtccggc
      721 ggcagcggtg catacggcga ctcgtcgcga ccgtggtgct cgcgcaccgc ggcagccacc
      781 gagtagcggt cgccgaaccg cgcggcgagc gcggtagcga gggaagcggg aaggggggca
      841 tgggatgcgg gcgcgttcat aatctccggc tccggccagt ttccggttaa cttccgaatt
      901 gacggccaca ccatacaccg ggcagatgat gggcaagcgg ctgaccaaga ttgcgacgcg
      961 taccggggac gacggaacga ccggcctggg cgacggcacg cgtacgcgca aggatgcgtt
     1021 gcgcgttgcc gcgatgggtg acgtcgatga actcaactcg acgctcggtg tgctgctgac
     1081 cgagccgatg ccggacgacg tgcgcgcgga tctcgtcgac atccagcacg atctgttcga
     1141 tctcgggggc gagctctgca ttccgggcta cacgatgatc cgggaggctc agatcgagcg
     1201 gctggatcag cgcctggaac actacaacgc gtcgttgtcg cgcctggccg agttcatcct
     1261 gccgggcgga tcgcgcgctg ctgccctcgc gcatgtgagc cgcacggtat gccggcgtgc
     1321 ggaacggtcc gtcgtcgcgc tgggcgcggc tgaacccgtg aatggggcgc cgcgccagta
     1381 cctgaaccgg ctgtcggacc tgctgttcgt tcttgcccgg gtgctgaacc gtcacgccgg
     1441 cggcagcgac gtgctgtggc aaccgcgcaa gaacgcggcg agccccgacg cctagcggcg
     1501 gctccgccgg cgcgtgtctg acccgacccg gcggcgcgct cggatcaacg tgctcggccg
     1561 cggtgcttca agcgtgccgc ttcatccggg atttccgcgt ttcgtcgcat ggccttgcgc
     1621 tccgtcgcat gcgggatggc caggccgaag tcctccctta tgctcgcgcg ctttgctgcg
     1681 cgcagcagcc cggaaactgg aaggaaacga tgaaattcac gacgagaaac gcgctggcag
     1741 cgatcctgct gggcgcgttc acagccccgt ttgcgccggt cgctgccgcc gaacccgtgc
     1801 ggccgctgat cccggtgttc gacgcggcaa caatcagcgc acgctgcgat ggcgaactgg
     1861 gggcggtgcg caagcagctg aaggcgacgg aagcacgcaa gggcgccggc ggcatttttg
     1921 ccgagctgaa tgcgctgacg atccattttg ccgactttgc ctacccggtg tacctgctgc
     1981 agaacgtcgc gccggacaag gcgacgcgcg atgcggcaca ggcctgcctg gagaagctcc
     2041 tgcccttcga gaccgaggtc taccaaagcg ttgccctgta tcagcgcgta caggcagtga
     2101 agccgaggga tttgactgaa acggtgtatc agcaggatct gttcgagaaa ttcgaggacg
     2161 gtggggcgac gctgccgccc gacaagcgca agcgcgcgaa ggaaatcatc gacgaactcg
     2221 agcgcatgtc gctcacgttc cagaagaaca tcaacgatga cgcaacgacg gtcatcatca
     2281 cgccggccga ggcggacgga atgcccgagg cctggaaggc ggcgcgcaaa cgcgatgaca
     2341 gtggcaactt cgtgctgggg atggactacc cgacctatgt accattcctc gaactggcca
     2401 cgaatgccga tgcgcgcaaa cgcgtctgga tggcgaagag ccgggagggt ggcgagaaga
     2461 acctgaagat cctcgagcaa gcgctgcagc tccgctacga actggcgcag cttcacggcc
     2521 aaccggactt tgccagctac gcgctcaagc gccgcatggc gcagacgccg gccgcggtct
     2581 tcgactttct cggcaaggtg aaggcggccg ccgacccgct gcaggagaag gagttcggcg
     2641 agctgcgggc cgagaaggcc aggctggacg gcacgccgct cgccgacacg aaactcaatc
     2701 gctgggacgt caacttcctg aaagagcgcg tgcgcaaggc gcgctacagc gtcgaccagg
     2761 aagcactgcg cgcgtacttc ccgaccgaag ccagcgtgca gtacgccatg cggctggcgg
     2821 accgcctcta cagcgttgag ttcgtcgcgc gcgacgtgcc gcgctggcac agcgatgtgc
     2881 gttactacga cgtctacgac cgcctgcccg acggcaagcg cggcgctttc gtcggcggca
     2941 tctacctcga tctgttcccg cgcgatggca aatacaacca tgcagctgcc tttccggtg
//
LOCUS       DATIRT010000205         5051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1798639, whole genome shotgun sequence.
ACCESSION   DATIRT010000205 DATIRT010000000
VERSION     DATIRT010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1798639"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VLN25_04855"
     CDS             complement(<1..511)
                     /locus_tag="VLN25_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HSQ09931.1"
                     /translation="MQATDIRRPDHFHRVVDCQWACPAHTPVPEYIRLIAGGRFADAY
                     MVNWKSNVFPGILGRTCDRPCEPACRRGRVEENQVARPEPVAICRLKRVAADFKEDIR
                     PRMPRRRVPMNGKRVACIGAGPASLTVARDLAPLGYEVIVFDQDPKAGGMIRTQIPRF
                     RLPEEVIDEE"
     gene            802..3660
                     /locus_tag="VLN25_04860"
     CDS             802..3660
                     /locus_tag="VLN25_04860"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HSQ09932.1"
                     /translation="MMRQFQVNSYLFGGNAPYVEELYERYLDNPGAVPEKWRAYFDQM
                     QLVPAADGSTSSRDVAHAPIVESFAQRAKAGAFQPKVAPTDLSVARKQVHVQSIIAAY
                     RYLGNRWANLDPLQRQERPSIPELEPAFYDLTEADMDTVFSASNTYFGQEQMTLREII
                     TALRQTYCGSIGAEYMYISDPAQKRWIQERLESVRSTPTFGAEDKMHILERVTAAEGL
                     ERFLHTKYVGQKRFSLEGGETFIAAMDELIQRAGSKGVEEIVIGMAHRGRLNVLVNTL
                     GKMPKELFAEFEGKPGSDLPAGDVKYHMGFSSDIPTPGGPVHLSLAFNPSHLEIVNPV
                     VEGSVRARQERRGDEAGDEVLPVLVHGDAAFAGQGVVMETLNLAQTRGYGTGGTIHIV
                     INNQIGFTTSDPRDSRSTIYCSDVVKMIEAPVFHVNGDDPEAVVFLTQLAMDYRQEFN
                     LDVVLDIVCFRRLGHNEQDTPAVTQPLMYKKIGGHPGTRKVYADKLVTQGTIAPEVPD
                     QMVKAYRAAMEEGRTTSDPVLTNYKSKYAVDWSAFLNRKWTDHADTGVPLAELKRLGE
                     RITTIPSTFKVHPLVEKVIADRRAMAEGKVPLDWGMGEHLAFASLVASGYAVRLTGQD
                     SGRGTFTHRHSVLHDQAREKWDEGTFIPLQHVSDTQAPFTIIDSVLSEEAVLAFEYGY
                     ASAEPNALVIWEAQFGDFVNGAQVVIDQFLSSGEVKWGRQSGLTLMLPHGYEGQGPEH
                     SSARLERFLQLAADNNMQIAQPTTAAQIFHLLRRQMIRLFRKPLVIMTPKSLLRNKDA
                     ASDLSELARGEFQTIISERDATIDAKKVKRIVLCSGKVYYDLAHARSERKEDDVALLR
                     VEQLYPFPHKALVAEFRKFPAATEIVWCQDEPANQGPWFYTQHHLLENMTDGQKLGYA
                     GRPASASPAVGYYAKHVEQQKALIDTAFGKLKGLVLNK"
     gene            3777..5027
                     /gene="odhB"
                     /locus_tag="VLN25_04865"
     CDS             3777..5027
                     /gene="odhB"
                     /locus_tag="VLN25_04865"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010809465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HSQ09933.1"
                     /translation="MAIVEVKVPQLSESVAEATMLQWKKKPGDAVVADEILIEIETDK
                     VVLEVPAPSSGALGEIIKGDGATVLSGELIATIDTEGKGAAAVAASPAAAVPKATAAA
                     PGASGVAMPAAAKLMADAGLAAGQVAGTGKDGRVTKGDVLGALAAGTQARAAPAPTPA
                     AAAPAARPALPEVKAPIDFSSLADRPEQRVPMSRLRQRVAERLVQSQSTAAILTTFNE
                     VNMQPLMDMRNRYKDRFEKEHGVKLGFMSFFVKAAVAALKKYPVLNASIDGNDIVYHG
                     YFDIGIAVGSPRGLVVPILRDADQMTFSDIEKKIADFGKRAGEGKLALEELTGGTFSI
                     SNGGVFGSMLSTPIINPPQSAILGVHATKDRAVVENGVVVVRPMNYLAMSYDHRIIEG
                     REAVLGLVAMKEALEDPARLLFDI"
BASE COUNT          949 a         1600 c         1684 g          818 t
ORIGIN      
        1 tttcctcgtc gatgacttcc tcgggtaaac ggaagcgggg aatctgggta cggatcattc
       61 cgccggcctt ggggtcctgg tcgaacacaa tgacctcgta accgagaggg gcaaggtcgc
      121 gcgcgaccgt cagcgaggcc gggccggcgc cgatgcaggc cacgcgcttg ccattcatcg
      181 gcacgcgtcg ccgaggcatg cggggacgga tgtcttcctt gaaatcggcc gccacgcgct
      241 tgaggcgaca gattgcgaca ggctcgggtc gggcgacctg gttttcctcg acgcgtccgc
      301 gtcgacaggc cggttcgcag ggtcggtcgc aggtccgccc caggattccc gggaagacgt
      361 tcgacttcca gttgaccatg taggcgtcgg cgaaacgccc tccggcgatc aggcggatgt
      421 actcgggaac aggggtatgc gcggggcagg cccactggca gtccacgacc cgatgaaagt
      481 ggtcgggacg acggatatcc gtcgcttgca cgtgtctcct cctcgaccgt cttggcggcg
      541 agtcatgatg tggcggcagt gtattccgct gaaggcgggc tgggcacgtc gagaagacga
      601 tgcggcgcat tggacacaaa cgttcgaaat ccgctccctc ggtggtagca tcccgcgctt
      661 gtcgccaggc cgcttgcctc aggccgacat actcgatggt cgtaccggaa tccggtgggg
      721 ccattgccgg gagcacgacg ccgcagggcg gagcgactgg gcatcttcaa cgagaaactc
      781 gttctgaaag gtgatggacc gatgatgcga cagttccagg tcaactcgta cctgttcggc
      841 ggcaatgcgc cgtacgtcga ggaactctac gagcgttacc tcgacaaccc cggcgcggtg
      901 cctgaaaaat ggcgcgcgta cttcgatcag atgcaactcg tgccggcggc cgatggctcg
      961 acgagctcgc gcgatgtcgc gcacgcaccg atcgtcgagt ccttcgccca gcgcgccaag
     1021 gccggagcct tccagccgaa ggtggccccg accgacctgt cggtggcgcg caagcaggtc
     1081 catgtccagt cgatcattgc ggcctatcgc tacctcggca accgctgggc caatctcgat
     1141 ccgttgcagc ggcaggaacg gccgtcgatt ccggagcttg aacccgcgtt ctatgacctg
     1201 accgaagcgg acatggacac cgtcttttcg gcgtccaaca cgtacttcgg gcaggaacag
     1261 atgacgctgc gcgagatcat caccgcgctg cgccagacct actgcgggag catcggcgcc
     1321 gaatacatgt acatctccga tccggcgcag aagcgctgga tccaggagcg gctcgagtct
     1381 gtgcgctcga cgccgacatt cggtgccgaa gacaagatgc acatcctcga gcgggtgacc
     1441 gcggccgaag gcctcgagcg cttcctgcac acgaagtacg tcgggcagaa gcgcttctcg
     1501 ctggaaggcg gtgagacctt cattgccgcg atggacgaac tgatccagcg cgccggctcg
     1561 aaaggcgtcg aggagatcgt catcggcatg gcgcaccgtg gccgccttaa cgtgctggtc
     1621 aacacactcg gcaagatgcc gaaggagctg ttcgccgaat tcgagggcaa gcccggctcc
     1681 gacctgccgg cgggcgacgt caagtaccac atggggtttt ccagcgacat cccgaccccg
     1741 ggcggtccgg tgcacctgtc gctggcgttc aatccgtcgc acctcgagat cgtcaacccc
     1801 gtggtcgagg gctcggtgcg tgcgcgacag gaacgccgcg gcgacgaggc gggcgacgag
     1861 gtgctgccgg tgctggtgca cggcgatgcc gccttcgccg gccagggcgt cgtcatggaa
     1921 acgctgaacc tggcgcagac gcgtggctac ggtaccggcg gcacgatcca cattgtcatc
     1981 aataaccaga tcgggttcac gacatccgac ccgcgcgact cgcgctcgac gatctactgt
     2041 tccgacgtcg tcaagatgat cgaggcgccg gtcttccatg tgaacggcga cgaccccgag
     2101 gcggtggttt tcctcacgca gctggccatg gactaccgcc aggaattcaa ccttgacgtc
     2161 gtgctcgaca tcgtctgctt ccgccgactc ggtcacaacg aacaggacac tccggccgtc
     2221 acgcagccgc tgatgtacaa gaagatcggc ggccacccgg gtacgcgcaa ggtgtatgcg
     2281 gacaagctgg tcacgcaggg cacgatcgcg cccgaggtac cggaccagat ggtcaaggcc
     2341 taccgggcgg cgatggaaga gggccgcacc acgtcggacc cggtgctcac caactacaag
     2401 agcaagtacg cggtcgactg gtccgcgttc ctgaaccgga agtggaccga ccatgcagac
     2461 accggcgtgc cactggccga gctcaagcgg ctcggcgagc gcatcacgac gatcccctcg
     2521 accttcaagg tgcatccgct ggtcgagaag gtgatcgcgg accgacgcgc gatggccgaa
     2581 ggaaaggtgc cgctcgactg gggcatgggc gaacacctgg cgtttgcgtc gctggtggcc
     2641 agtggctatg ccgtgcgcct caccggccag gactccggtc gcggcacctt cacccaccgc
     2701 cattcggtgc tccacgacca ggcccgcgag aagtgggacg agggcacgtt cattccttta
     2761 cagcacgtgt cggacactca ggcgcctttc acgatcatcg attccgtgct gtcggaagag
     2821 gccgtgctgg cgtttgagta cggctacgcc agcgcggaac cgaatgcgct ggtgatctgg
     2881 gaagcccagt tcggcgactt cgtcaacggc gcacaggtgg tgatcgacca gttcctgtct
     2941 tccggggaag tcaagtgggg ccggcagtca gggctcacgc tgatgctgcc gcatggctat
     3001 gagggccagg gccccgaaca ttcgtcggcg cggctggagc gcttcctgca gctcgcggcc
     3061 gacaacaaca tgcagatcgc ccagccgacg acggccgcac agatcttcca cctgctgcgc
     3121 cggcagatga tccggctttt ccgcaagcca ctggtgatca tgacgccgaa gtccctgctg
     3181 cggaacaagg atgcggcgtc ggacctgtcg gaactcgcgc gtggcgagtt ccagacgatc
     3241 atcagcgagc gtgacgcgac gatcgatgcg aagaaggtca agcgcatcgt gctgtgctcg
     3301 gggaaggtct attacgacct tgcgcacgcg agaagcgagc gcaaggagga cgatgtcgcg
     3361 ctgttgcgcg tcgagcagct gtacccgttc ccgcacaagg cgcttgttgc ggagttccgc
     3421 aagttcccgg cggccacgga aatcgtctgg tgccaggacg aaccggccaa ccagggcccg
     3481 tggttctaca cccagcacca cctgctggag aacatgaccg acggccagaa gctcggctac
     3541 gcggggcggc ctgcatctgc atcgccggcg gtcggctact acgccaagca tgtcgagcag
     3601 cagaaggcgt tgatcgacac cgcgttcgga aaactcaagg gcctcgtgct gaacaagtga
     3661 cgttccggac gcgcccagcg tttcggaaca tcgaacgcac gacccggcgg cctgccgcct
     3721 gactcgcgcg gcgacacccg gtgcagcccg cgtcccccat tggagccagg aagaaaatgg
     3781 caatagttga agtcaaggta ccgcagctgt cggaatcggt cgccgaagcc acgatgctgc
     3841 agtggaagaa gaaacccggc gatgcggtgg tcgccgacga gatcctgatc gagatcgaga
     3901 ccgacaaggt cgtgctggaa gtgccggcgc cgtcgagtgg ggcacttggc gagatcatca
     3961 agggcgatgg cgccacggtg ttgtcgggcg aactcatcgc gacgatcgac acggaaggca
     4021 agggggccgc ggccgtggcg gccagtccgg cggctgccgt accgaaggcg actgcggcgg
     4081 cgcccggagc atcgggggtg gcgatgccgg cggcggcgaa gttgatggcc gatgccggac
     4141 tcgcggccgg gcaggtcgcc gggaccggaa aggacggccg cgtgaccaag ggcgacgtac
     4201 tcggcgcgct cgccgcgggc acgcaggcgc gcgcagcacc tgcgccgact cccgcagcgg
     4261 cggcgccggc ggcgcggccc gcgcttccgg aagtcaaggc gccgattgac ttctcgtcgc
     4321 tcgccgatcg gccggagcag cgcgtgccga tgtcgcgatt gcgccagcgg gtggccgaac
     4381 gtctcgttca gtcgcagtcg acggcggcca ttttgaccac gttcaacgag gtcaatatgc
     4441 agccgctgat ggacatgcgc aatcgctaca aggaccgctt cgaaaaggag cacggcgtga
     4501 agctcggctt catgtcgttc ttcgtcaagg ccgcggtggc ggcgctgaag aagtacccgg
     4561 tcctcaatgc gtccatcgac ggcaacgaca tcgtttatca cggctacttc gacatcggca
     4621 tcgcggtcgg ttcgccgcgc ggcctggtgg tgccgatcct gcgcgatgcc gaccagatga
     4681 cgttctccga catcgaaaag aagatcgccg acttcggcaa gcgcgcgggc gaaggcaagc
     4741 tcgcgctcga ggaactcacc ggcggcacct tctcgatctc caacggcggc gtgttcggct
     4801 cgatgctcag cacaccgatc atcaacccgc cgcagagcgc gatcctcggc gtgcacgcga
     4861 ccaaggaccg cgctgtggtc gagaacggcg tcgtcgtggt gcgccccatg aactacctgg
     4921 cgatgagcta cgaccaccgc atcatcgaag gccgcgaagc ggtgctcggg ttggtggcga
     4981 tgaaggaagc gctggaggat cccgcccgcc tgctcttcga catatgaacg gatggataac
     5041 gcggcctgaa g
//
LOCUS       DATIRT010000206         3713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1503824, whole genome shotgun sequence.
ACCESSION   DATIRT010000206 DATIRT010000000
VERSION     DATIRT010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1503824"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VLN25_04870"
     CDS             <1..773
                     /locus_tag="VLN25_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HSQ09934.1"
                     /translation="FLGRVWNRPPLWSEVFAHYMTFARMVLDRVYWLSRAEHGIPVRV
                     HGAEVVEAALARRTAGLIVVGSHLGSFEALRGAGERVCGRRIRPLMYVANARKVQRVL
                     DAINPELKRDVIFAGRTGTMLEIREAIEQGHIVGILADRSPFAERTMTANFMGQPAPF
                     PVGAYRLAALLGVPVVFSCAVLSGKGYEIYFEAMGEPPAGGSRVARERWIVEQVARFV
                     RHLEHHARTYPLNWFNFYDFWSGGSTQSSSSGAAGPSP"
     gene            770..1330
                     /locus_tag="VLN25_04875"
     CDS             770..1330
                     /locus_tag="VLN25_04875"
                     /inference="COORDINATES: protein motif:HMM:NF039988.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HSQ09935.1"
                     /translation="MTVVAACVAALAMMLSSVAAAQEPITPAEILHRVSAAVSPEVGF
                     REVRTTRLLKTPLVSTGVLRYSPPGRLERETRKPMQEFVVIEDLQVTIDRGGSQTVVS
                     LISGTAPAAMVQTLRAVLGGQMRELDALYQASAQGSSERWTLNLEPRAVDTAVRSIRL
                     LGSGGAVEEIEVLERSGDKTVTQLSR"
     gene            1344..3686
                     /locus_tag="VLN25_04880"
     CDS             1344..3686
                     /locus_tag="VLN25_04880"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HSQ09936.1"
                     /translation="MGRSLRLALWCILAVALGFALPQVTVDADLSRFVSADRPDAQLD
                     LSVALQRGMGGRAVLIALPASTTKAAADVSRRMVTALRISGVFATVHNGDTGLLAAEV
                     EPLIAYRYVLSDRVDAALFSEAGLRMRLQDAEAMLADARAWIVTQLLPIDPTLETLHL
                     AEQWGGSARLPMQHGVWFARSGTDAMIIATTRAAGDDAVGQKAVEAAIRQAASTVGVP
                     QVKYTGLGLLAAEARDATRARVERLGWVSALLVTFILYLGYRRLLPVVLSLLPVALGI
                     AAGGVVTDLIFGDINVLAVAFACILVDEGSDYASYLLTQARPGRPLGAEARRIWPTLR
                     LAVLTSTAAFAVLLLAQFRGLQQLGLLCGVGLLVAGAAARWLLPDLLNPAHMASWSPP
                     RVARDVPAGSRWSLPTGRRGRMVKLAAAVGALLVLSVARPTWDDGVAAINPLPTDRIA
                     ADRALRSAAGLPLDQGVLLFSGADDEAVLQAQEAWLPALRALQLQGEVETFDLAARYF
                     PSFRTQQQRLAAVPDASILAPLLERAVQGTAFNVEAFQPFLESASKARSTALTLSALP
                     DGLFRQRVESLLLQIDGQSLGLVPIAGRAARDDLIASARKAVAGRPVTVSWFEPRAQL
                     SVLLIAVRERLLGLLLLCVVAMVLVIAIDRRSLALGVRVMTPVVTALLLTAALVRLGF
                     GPLTVFNLVALMLVLGVITNYSLFIHSPPAESDESDRRAHLVFSLLIASATTLAVFGA
                     LGLSGIRVVEAVGQTVVVGIIAGLAWLIATRGPAAGGAGK"
BASE COUNT          519 a         1156 c         1311 g          727 t
ORIGIN      
        1 actttctggg gcgtgtgtgg aaccgacccc cgctctggtc ggaagtgttc gcgcattaca
       61 tgaccttcgc gcggatggtg cttgaccgcg tctactggct gtctcgcgcc gagcacggca
      121 ttccggtacg agtgcacggg gccgaggtgg tcgaggcggc gcttgcgcgg cggacagccg
      181 gtcttatcgt agtcggttcg catctcggaa gcttcgaggc actgcgcggc gccggtgagc
      241 gagtctgcgg tcgtcgtatc cgcccgctga tgtatgtcgc caatgcgcgc aaggttcagc
      301 gggtgctcga tgcgatcaac ccggagttga aacgcgatgt gatcttcgcc ggccgcacgg
      361 gcacgatgct cgagattcgc gaggcgatcg agcagggcca cattgtcggg atcctggcgg
      421 atcgatcgcc gtttgccgaa cgaacgatga ctgcgaattt catgggccag ccggcaccgt
      481 tccctgtcgg tgcctaccgg ctcgcagcgc tgctcggggt cccggtcgtg ttctcgtgcg
      541 cggtgctgag tggcaagggc tacgagatct acttcgaggc gatgggcgaa ccgcccgccg
      601 gcggctcgcg cgtcgctcgc gagcgctgga tagtcgagca ggtcgcccgc ttcgtgcgcc
      661 atctggaaca ccatgcccgc acgtacccgt tgaactggtt caacttctac gacttctggt
      721 ccgggggttc gacgcagtca tcgtcgtccg gggcggccgg gccttcgccg tgaccgtcgt
      781 cgcggcctgc gtcgctgcgc tcgcgatgat gctgtcgtca gtcgcggcgg ctcaggagcc
      841 catcacaccc gcagagatac ttcatcgcgt ttccgccgcg gtttcccctg aagtgggttt
      901 cagggaagtg cggacgacgc gtctgctgaa gacgccgctg gtgtccaccg gtgtgctgcg
      961 ctattccccg ccgggccggc tcgagagaga aacgcggaag ccgatgcagg agtttgtcgt
     1021 catcgaagac ctgcaggtca caatcgatcg cggtggatcg caaacggtgg tctcgctgat
     1081 cagcggaaca gctcctgcag cgatggtgca gacgctgcgc gcggtactcg gtggccagat
     1141 gcgcgagctc gacgcgctgt accaggcgag cgcgcagggt tcgagcgagc gatggacgct
     1201 gaacctggag ccacgcgcgg tcgacaccgc ggtccgcagc attcggctgc ttggcagcgg
     1261 gggggcggtg gaggagatcg aagtactgga acgaagcggg gacaagaccg tcacccagct
     1321 ttcgcgctag gccggacgcc ggcgtgggtc gctccctccg tcttgccctg tggtgcatcc
     1381 tggccgtggc gcttgggttc gcacttccgc aggtgactgt cgacgctgat ctcagccgct
     1441 tcgtatcggc cgataggccc gatgcacagc tcgacttgtc ggtcgcactg cagcgcggaa
     1501 tgggggggcg ggcagtcctg atcgcgcttc ctgcgtcgac cacgaaggcg gccgccgatg
     1561 tcagccgccg catggtgaca gcgctgcgaa tctctggtgt gtttgcgacg gtgcacaacg
     1621 gagatacggg actgctcgcc gcggaggtcg agccgctgat cgcttaccgc tacgtgctca
     1681 gcgatcgcgt cgatgccgcg ttgttctcgg aagccggact tcgcatgcga ctgcaggatg
     1741 ccgaggccat gcttgccgat gcgcgcgcct ggatcgtgac gcagctgctt ccgatcgatc
     1801 cgacgctcga aacgctgcat ctggcagagc agtggggcgg cagcgcgcga ttgccgatgc
     1861 agcacggggt gtggttcgcc cgcagcggca cggacgccat gatcattgcg acgacgcgcg
     1921 ctgcgggtga cgatgccgtt ggccagaagg ctgttgaagc agccatccgg caggcggcct
     1981 cgacggtcgg cgtaccgcag gtgaagtaca ccggcttggg actgctggct gccgaggcgc
     2041 gtgacgcgac tcgcgcgcgg gtcgagcgac ttgggtgggt cagtgcgctg ctcgtcacgt
     2101 tcattctcta cctgggatat cgtcggttgc tgccggtcgt gctgtcgctg ctgccggtag
     2161 cgctgggaat agcggccggt ggcgtcgtga ccgacctcat cttcggcgac atcaacgtgc
     2221 tggcggtcgc cttcgcgtgc atcctcgtcg atgaaggctc cgactacgcg tcctatctgc
     2281 tgacgcaggc gcgacccggg cggccgctgg gtgctgaagc tcgccgcatc tggcctacct
     2341 tgcgacttgc ggtgctgacc agtactgctg cttttgcggt gctgctgctg gcccagtttc
     2401 gtggcctgca gcagctcggc ctgctgtgcg gcgtcgggct gttggttgcg ggcgcggcgg
     2461 cacggtggtt gctccccgac ctgctcaatc ctgcacatat ggcgagctgg tcgccgccgc
     2521 gggtcgcgcg cgacgtcccg gccgggtctc gctggagcct gccgacaggc cggcgcggac
     2581 gcatggtgaa actggctgcg gcggttggag cgctgctcgt gctgtcggtc gcgcggccga
     2641 cttgggacga tggcgtggca gccatcaatc cgctgccaac cgaccgcatc gctgctgacc
     2701 gcgcgcttcg cagtgcggcc ggtctgccgc tcgaccaggg cgtgttgctg ttttcgggtg
     2761 ccgacgacga agcggtgttg caggcacagg aggcatggct gcctgcactg cgcgcattgc
     2821 agcttcaagg tgaggtggag accttcgacc tggccgcacg ctattttccg agtttcagga
     2881 ctcagcagca gcgcctggca gccgtgcccg acgcctcgat attggcgccg ctgcttgaac
     2941 gggcggtaca gggcacggcg ttcaatgtgg aagccttcca gccatttctc gaaagcgcgt
     3001 cgaaggcgcg ttcgaccgcg ctcacgctgt ccgcactgcc cgacggcttg ttcaggcaac
     3061 gcgtcgagag cctgctgctg cagattgatg gccagtcgct tggactggtg ccgattgcgg
     3121 ggcgcgccgc gcgcgacgac ctaatcgcct cggcccgcaa ggctgtcgcg ggtcggccag
     3181 tcaccgtgtc ctggttcgag ccgcgggcgc agctgagtgt gttgctgata gcggtgcgcg
     3241 aacgcctgct gggactgctg ctgctctgcg tggtcgcaat ggtcctcgtc atcgcaatcg
     3301 accggcgctc gttggccctc ggcgtgcgag tgatgacccc cgtagtcact gcgctcttgc
     3361 tgacggcggc gctcgtgcgg ctcggattcg gacccctgac tgtgttcaat ctcgtggcgt
     3421 tgatgctggt cctgggtgtc atcaccaact attcactgtt cattcactcg ccgccggccg
     3481 agtcggatga aagcgatcgg cgggcgcatc tggtgttttc gctgctcatt gcgagtgcga
     3541 ccaccctggc ggtgttcgga gcgctcgggc tcagcggcat ccgagtcgtc gaggcggtcg
     3601 gccagacggt ggtcgtcggt atcattgcgg ggctcgcgtg gctcattgcg acgcgcgggc
     3661 ccgccgccgg cggcgcagga aagtagccgg gtgcattcat cgaatccgtc gtg
//
LOCUS       DATIRT010000207         1969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_717627, whole genome shotgun sequence.
ACCESSION   DATIRT010000207 DATIRT010000000
VERSION     DATIRT010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717627"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VLN25_04885"
     CDS             <1..693
                     /locus_tag="VLN25_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018314692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSQ09937.1"
                     /translation="KSIYDIDFEFLPEFQSNLDRHPKVHGFSIIDHMTHNVYRGRMAF
                     WGSFYERIFNFREIRYFDIKGEYTGLTSRAMTAPDGKIRIPLNEESGKGGTGQIEEFL
                     MKFNGEGIQHIALLTDDLMASVDSLQMAGVPLMSAPNDIYYEMLEERLPGHGEPVAQM
                     KARGILLDGSTQGGEKKLLLQIFSETMLGPVFFEFIQRKRDDGFGEGNFKALFESLER
                     DQIRRGALTVDA"
     gene            686..991
                     /locus_tag="VLN25_04890"
     CDS             686..991
                     /locus_tag="VLN25_04890"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HSQ09938.1"
                     /translation="MPRIPRLGTAQVRARLTALPGWTLSDDGLAITRQFKFADFQQAF
                     AFMTQVALAAERADHHPEWSNVYNRVDMRLTTHDAGGLTESDFALAAVADAAAGTAT"
     gene            988..1884
                     /gene="phhA"
                     /locus_tag="VLN25_04895"
     CDS             988..1884
                     /gene="phhA"
                     /locus_tag="VLN25_04895"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004505 - phenylalanine 4-monooxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HSQ09939.1"
                     /translation="MNAATDKHGLAAGAAGRPQSADWTIPQRWADYTAGEHAVWKVLY
                     ERQTALLRGRACNEFVAGMHALPIAPDRIPDFEHLSEVLGSHTGWQIVAVPGLVPDEV
                     FFEHLAHRRFPSGQFIRRADQLDYLEEPDVFHDVFGHAPMLMNPVIADFIQAYGEGGL
                     RAQRFGTLPLLARVYWYTVEFGLVRQSDGLRIYGSGIASSRTESVFSLDDDSPHRIGF
                     DLERVMCTRYRIDDFQECYFVLDRLDDLLELAHIDFAPVYERVQRRDEIEPGQLLPED
                     RIVTRGSGRYHAARRAGARHAI"
BASE COUNT          349 a          652 c          652 g          316 t
ORIGIN      
        1 aagagcatct acgacatcga cttcgagttc ctgcccgagt ttcaatcaaa cctggatcgc
       61 catccgaagg tccacggctt cagcatcatc gatcacatga cgcacaacgt gtaccgtggg
      121 cgcatggcgt tctggggcag cttctacgag cgcatcttca acttccgcga gatccgctat
      181 ttcgacatca agggcgagta cacggggctc acgagccgcg cgatgacggc gcccgacggc
      241 aagatccgca tcccgctgaa cgaggagtcg ggcaagggcg gcaccgggca gatcgaagag
      301 ttcctgatga agttcaacgg tgaaggtatc cagcacatcg cgctgctgac cgatgacctc
      361 atggccagcg tcgattcgct gcagatggcc ggcgtgccgc tgatgagcgc tcccaacgac
      421 atctactacg aaatgctgga ggagcggctg ccgggccacg gcgagccggt ggctcagatg
      481 aaggcgcgcg gcatcctgct cgacgggtcg acccagggcg gagagaagaa gctgctgctg
      541 cagatcttct ccgagacgat gctcggaccg gtgttcttcg agttcatcca gcgcaagcgc
      601 gacgacgggt tcggcgaagg caatttcaag gcgctgttcg agagcctgga gcgcgaccag
      661 atccgccgtg gcgcgctgac cgtcgatgcc taggatccct cggctcggca cggcgcaggt
      721 acgcgcgcgg ctgaccgccc tgcccggctg gacgctgagc gacgacggac tggcgatcac
      781 gcggcagttc aagttcgccg atttccaaca ggccttcgcg ttcatgacgc aggtggcact
      841 cgcggccgag cgcgccgacc atcaccccga gtggagcaac gtctacaacc gcgtcgacat
      901 gcggctgacg acgcacgatg cgggcgggct gaccgagagc gacttcgcgc tggccgccgt
      961 ggcggatgcc gcggccggca ccgcgacatg aacgctgcca ccgacaagca cggactggcg
     1021 gccggagcgg ccggacgccc gcaaagcgcc gactggacca tcccgcaacg ctgggccgat
     1081 tacacggcgg gcgagcacgc ggtgtggaaa gtgctctacg agcgccagac cgcactgctg
     1141 cgcggacgcg cctgcaacga attcgtcgcc ggcatgcacg cgctgccgat cgcgcccgat
     1201 cgcattccgg acttcgaaca cctgagcgag gtgctgggca gccacaccgg ctggcagatc
     1261 gtcgcggtgc cggggcttgt gcccgatgag gtctttttcg agcacctcgc gcaccggcga
     1321 tttccgtcgg gacagttcat tcgccgcgcc gatcagctcg attacctgga agagcccgac
     1381 gtcttccacg atgtgttcgg tcacgcgccg atgctgatga acccggtgat cgcggacttc
     1441 atccaggcct acggcgaagg cggcctgcgc gcgcagcggt tcggcacctt gccgctgctg
     1501 gcgcgcgtgt actggtacac ggtggagttc ggactcgtca ggcagtccga cggcctgcgc
     1561 atctacggct ccggcatcgc cagctcgcgc accgagagcg tgttctcgct ggacgacgat
     1621 tcgccgcacc gcatcggctt cgacctggaa cgcgtgatgt gcacgcgcta ccgcatcgac
     1681 gacttccagg agtgctactt cgtgctggat cggctcgacg acctgctcga actcgcgcac
     1741 atcgacttcg cgccggtgta cgagcgcgtg cagcgacggg acgagatcga accggggcag
     1801 ttactgcctg aggaccggat cgtcacgcgc ggcagcggcc gctatcacgc cgcgcgccgt
     1861 gcaggagcca gacatgccat ctgacggatg cgtgctcgtc accggggcag cgggcgcact
     1921 cggccgcgcg gtcgttgccc acttcgctgc ccgcggcgcc aggctggcg
//
LOCUS       DATIRT010000208         2771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1307335, whole genome shotgun sequence.
ACCESSION   DATIRT010000208 DATIRT010000000
VERSION     DATIRT010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307335"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="VLN25_04900"
     CDS             complement(<1..1126)
                     /locus_tag="VLN25_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HSQ09940.1"
                     /translation="MASITVAQFATELKVPPQLLIEQLRSAGVVKEAEGDPLSDDDKA
                     RLLESLRKAHGGEAAEKKKITLTRKQTTEIKQADASGKARTIQVEVRKKRVFVKRDEA
                     APEPEAPVAPAAPVIDEAELAKREEEARRQAEFTARQMQEARERAEHAQRELAAAQTK
                     EQHDVEGAREREAAAAAELARAEQAQRDADRAAKEAVAAQQRARRAVEDEVAAIKSMM
                     SAPKKVVKPAEPAATAATAGTLHKPTTKPESKPGEGKKDDKKPGVKEIKSGKLASSWS
                     EEQQKKRQLKTRGDSSGGTGAWRGPKGHRKHGRDESDGAAQAMPAEPTVRDVSVPETI
                     TVAELAHKMSVKAAEVIKVLMKLGQMVTINQVLDQDTAMII"
     gene            complement(1169..2665)
                     /gene="nusA"
                     /locus_tag="VLN25_04905"
     CDS             complement(1169..2665)
                     /gene="nusA"
                     /locus_tag="VLN25_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HSQ09941.1"
                     /translation="MSREILLLVDALAREKNVPKETVFGALESALAMATKKLFAEEDV
                     DIRVAVYRETGEYDAYRRWLVVPDEAGLQEPGHHIILSDARDQIADIEIEEHIEELLP
                     RQDLSTGRRFAQDTKQVILQKIRDAEREQLLSDFLARGDQIVSGTIKRMDKGDAIIEI
                     GKVEARLPRTEMIPKENLRIGDRCRAFVQKIDRLARGPQVILSRTSPEFLMKLFEFEV
                     PEIEQGLLVIKSAARDPGVRAKIAVWTNDRRIDPIGTCVGMRGSRVQAVTNELAGERV
                     DIVLWSDDPAQFVIGALAPANVSSIVVDEDKHAMDVVVDEDNLAIAIGRSGQNVRLAA
                     EMTGWQINIMTAEESAQKQESEQVGVRQTFIEKLDVDEEIANILIQEGFSTLEEIAYV
                     PVNELMEIEAFDEETVEELRNRARNALLTEAIKREENLEQAEKDLLDLEGMDADLAAR
                     LAENSIRTRDDLGELAVDELTEMTGIDDERAKGLIMAARAHWFESAEQ"
BASE COUNT          377 a          945 c          835 g          614 t
ORIGIN      
        1 cgatgatcat cgcggtgtcc tgatcgagca cttggttgat cgtgaccatc tgaccgagct
       61 tcatcagcac cttgatgacc tcggccgcct tgacggacat cttgtgggcc agttcggcca
      121 cggtgatcgt ctccggcaca gagacgtccc gcaccgtcgg ctcggccggc atggcctgcg
      181 ccgcaccgtc gctttcgtcc cgaccatgct tgcgatgtcc cttgggaccg cgccaggcgc
      241 cggtgcctcc cgaggaatcg ccacgcgtct tgagctggcg cttcttctgc tgctcttcgg
      301 accagctgga cgcgagcttg ccgctcttga tttccttgac gcccggcttc ttgtcgtcct
      361 tcttgccttc gcccggcttg gattcaggct tggtggtggg cttgtgcaac gtacctgccg
      421 tcgctgccgt agcggcgggt tcggccggct tcaccacctt cttgggcgcc gacatcatgg
      481 acttgatggc ggcgacttcg tcttcaactg cgcggcgagc tcgttgctgt gcagcgaccg
      541 cctccttggc ggcacggtcc gcatcacgtt gcgcctgctc ggcacgcgca agctcggctg
      601 cggccgcagc ttcgcgctcg cgcgcgccct cgacgtcgtg ctgttctttc gtctgagccg
      661 cagcaagctc gcgctgcgcg tgttcggcgc gctcgcgcgc ctcctgcatc tggcgcgcag
      721 tgaactcggc ctggcgacgc gcttcctctt cgcgcttggc gagctcggct tcatcgatca
      781 ccggcgcggc cggtgcaacg ggcgcctcgg gctccggtgc ggcctcatcg cgcttgacga
      841 acacgcgctt cttgcgcacc tcgacctgga tcgtgcgggc cttgccggac gcatccgcct
      901 gcttgatttc ggtggtttgc ttgcgcgtca gcgtgatctt cttcttctca gcggcctcgc
      961 caccgtgggc tttgcgcagt gactccagca ggcgcgcctt gtcgtcgtcg gacagtgggt
     1021 cgccctctgc ttccttgacg actccggccg acctgagttg ctcgatcagc aactgcggcg
     1081 gaaccttgag ttccgtcgca aactgggcca cggtgatgct tgccatagag ttccttatcc
     1141 ctgctgtccg tccagcccgc gccgccggct actgctccgc cgattcgaac cagtgcgcgc
     1201 gagcggccat gatcagcccc ttcgcgcgct cgtcgtcgat gccggtcatt tccgtcaact
     1261 cgtcgactgc cagctcgccg aggtcgtcgc gcgtgcgtat cgaattctcg gcaagcctgg
     1321 ccgcgaggtc cgcgtccatg ccctcgagat cgagcagatc cttctcggcc tgctcgaggt
     1381 tctcttcacg cttgatggct tccgtcagca gcgcattgcg cgcgcggttg cgcagttctt
     1441 ccaccgtttc ctcgtcgaac gcctcgattt ccatgagttc gttgacggga acgtaggcga
     1501 tttcctcgag cgtcgagaaa ccttcctgaa tcaggatgtt ggcgatctcc tcgtcgacgt
     1561 cgagcttctc gatgaacgtc tggcgcacgc cgacctgctc gctttcctgc ttctgggcgg
     1621 attcctcggc cgtcatgatg ttgatctgcc agccagtcat ttcagcagcc agcctgacat
     1681 tctgtccgct gcggccgatc gcgatcgcga gattgtcctc gtcgaccacg acgtccatcg
     1741 cgtgcttgtc ctcgtcgacg acgatcgagc tgacgttggc cggtgccagc gcgccgatca
     1801 cgaattgcgc cggatcgtcc gaccacagca cgatgtcgac gcgctcgccg gccagttcat
     1861 tggtcacggc ctgcacgcgc gagccgcgca tgccgacgca ggtgccgatc gggtcgatgc
     1921 ggcggtcgtt ggtccagacg gcgatcttgg cgcgcacgcc cggatcgcgc gcggccgact
     1981 tgatcaccag cagtccctgt tcgatctccg gcacctcgaa ctcgaaaagc ttcatcagga
     2041 actcgggcga ggtgcgtgac aggatcacct ggggtccgcg cgcgagacgg tcgatcttct
     2101 gcacgaaggc ccggcagcga tcgccgatac gcaggttttc cttcgggatc atctcggtgc
     2161 gaggcaggcg tgcttcgacc tttccgatct cgatgatggc gtcgcccttg tccatgcgct
     2221 tgatggtgcc gctgacgatc tggtcgccgc gggccaggaa gtcggacagc aattgctcac
     2281 gctcggcgtc gcggatcttc tgcaggatca cctgcttggt gtcctgcgcg aagcggcgcc
     2341 cggtagagag gtcctggcga ggcagcaatt cctcgatgtg ctcctcgatc tcgatgtcgg
     2401 cgatctggtc gcgcgcatcc gacaggatga tgtggtggcc gggttcctgc agacccgctt
     2461 cgtcgggcac gacgagccaa cgacgatagg cgtcgtactc gcccgtctcg cgatagaccg
     2521 cgacgcggat gtcgacgtct tcctcggcga acagcttctt cgtggccatc gccaacgccg
     2581 attcgagcgc cccgaaaacg gtttccttcg gaacgttctt ttcgcgcgct agtgcatcga
     2641 ccaacagcag tatctctcga ctcattgcgg tctactcctt gctcccgccc ctgaagttca
     2701 attcaggtac cagcttcgct ttgtccacct cgccaagcgc gaactccacg atcgcacccg
     2761 gcgcgggttc a
//
LOCUS       DATIRT010000209         2503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_324438, whole genome shotgun sequence.
ACCESSION   DATIRT010000209 DATIRT010000000
VERSION     DATIRT010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324438"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..394
                     /locus_tag="VLN25_04910"
                     /pseudo
     CDS             149..394
                     /locus_tag="VLN25_04910"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
     gene            404..2131
                     /locus_tag="VLN25_04915"
     CDS             404..2131
                     /locus_tag="VLN25_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HSQ09942.1"
                     /translation="MSAIPADMNVSRAPSRAALAGEALAARGGLVLLAVALFVFLTVP
                     LAMILVRAVEGRDGGFVGVANFIQYVRSPALAQSTLNTLEFALLTTVVTVPVAFLFAY
                     AIQRSCIPGKGLWRNIAMIPILAPSLLAAISFIYLFGNQGAFKFMLEWVGLKSIYGMP
                     GMVLAMTFASFPHAVMILMAALALSDARLYEAADSLGTNEPRKFFTITLPGAKYGLVS
                     ACMVVFTMAVSEFGVPKVIGGNTSVLAVDIYKQVIGQQNFSMGAVVGLVLLLPAVVAF
                     VIDNLMQRRQQALLTARSVPYQARRAPARDGLLLVFVVLISALLLLVVGMAAFGSVVK
                     FWPYNLSLTLNHYTYGFEEAGIEHAYRNSLVMALWCGVLGAAITFAGAYWVEKTRGSS
                     LVRPLIRFQAMLPMAVPGMVLGIGYILFFNLPTNPLNFLYGSMAILVISTIVHFYSSS
                     HLTALTALKQIDSEFESVSASLKVPFYKTFWRVTVPVCLPSILDIGRYYFVNAMTTIS
                     AVVFLYSPKTTLASISILQLDEAGAIGSAAAMATLIVVTSAVVTGVLFAIEWWLIRRT
                     QSWRNTALQ"
     gene            2148..>2503
                     /locus_tag="VLN25_04920"
     CDS             2148..>2503
                     /locus_tag="VLN25_04920"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308920.1"
                     /note="catalyzes the formation of phosphonoacetaldehyde
                     from 2-aminoethylphosphonate and pyruvate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HSQ09943.1"
                     /translation="MIVGNDPILLTPGPLTTSLATKAAMLRDWGSWDDAFNAITASLR
                     RDLVDIVSGSSEYTCVPLQGSGTFSVEAAIGSIVPREGKVLVPNNGAYCERIVRICGY
                     LGRAVVDLPVPEDQPAT"
BASE COUNT          390 a          770 c          815 g          528 t
ORIGIN      
        1 gtttgtggcc gacttcctcg gcaaggccaa tgtcctcaag ggagtcgcgc tcggcgcggg
       61 acgctatcgc gtgggatcgg ccgaactggc gctgccggcc gatgagcatc cggtcggttg
      121 cagtgtgcgg atctaagtcc ggcccgagga ccggcatgtc gagggcgaca ttgcatcgtt
      181 gccgaaccgc ctgcggggcc ggatcgcgcg catcgactat ctcggcacgt tctgcctggc
      241 cgaactgcgc tgcgaagcgc tcggccagcc gatgctgatc tcctactcgc tcaaccagtt
      301 gcatgatctc gccgtccgtg agggcggcga agtcgagatt gcgctgcgcg tcgatcgtgt
      361 gcgtgtcttc gcggataccg ccactgccgc ctgagaacgc ccggtgagtg ccattcctgc
      421 cgacatgaac gtgagccgcg cgccgtcgcg cgcagcgctc gcgggcgagg cgctggctgc
      481 gcgcggcggc ctggtgctgc tggcagtcgc gctcttcgtt tttctcaccg taccgctggc
      541 gatgatcctc gtgcgtgcgg tcgagggacg cgatggcggg ttcgttggcg tggcgaactt
      601 cattcagtac gttcggtcgc ccgcgctggc gcaatcgacg ctcaatacgc tcgagttcgc
      661 actgctgacc acagtggtca cggtgccggt ggcgttcctg tttgcctacg cgatccagcg
      721 cagttgcatt ccgggcaaag gtctgtggcg caatatcgcg atgatcccga tcctggcgcc
      781 gtcactgctc gctgcgatct cgttcatcta tctgttcggc aaccagggtg cgttcaagtt
      841 catgctcgag tgggtcgggc tgaagtcgat ttacggcatg cccggcatgg tgctggcgat
      901 gaccttcgca tcgttcccgc atgcggtgat gatcctgatg gccgcgttgg cgctctcgga
      961 cgcgcgcctg tacgaggcgg ccgattcgct cgggacgaat gagccccgca aatttttcac
     1021 gatcacgctg ccgggggcca agtacggact cgtttccgcc tgcatggtcg tgttcacgat
     1081 ggcggtgtcg gagttcggcg tgcccaaggt gatcggcggc aacacgagcg tgcttgccgt
     1141 cgacatctac aagcaggtca tcggccagca gaatttttcg atgggagcgg tcgtcggact
     1201 ggtgctgctg ctgccggccg tcgtggcctt tgtcatcgac aacctgatgc agcgccgcca
     1261 gcaggcactg ctgacggcac gcagcgtgcc gtaccaggcg cgccgggcgc cggcacgtga
     1321 tggactcctg ctggtgttcg tggtcctgat ctccgcgttg ctgctgctgg tcgtcggcat
     1381 ggcggcgttt ggatcggtgg tcaagttctg gccctacaac ctgtcgctca cgctcaatca
     1441 ctacacctac ggtttcgagg aagccggaat cgaacatgcc tatcgcaaca gcctggtgat
     1501 ggcgctgtgg tgcggtgtgc tcggggctgc aatcaccttt gccggcgcgt actgggtgga
     1561 aaagacccgc ggttccagtc tcgtgcggcc gctgatccgg ttccaggcca tgttgccgat
     1621 ggcggtgccg ggaatggttc tgggcatcgg ctacatcctg ttcttcaacc tgccgacgaa
     1681 tccactgaac ttcctgtacg gcagcatggc gatcctggtg atctcgacga tcgtgcactt
     1741 ctactcgtcg agccacctga ctgcgctgac ggcgctcaag cagatcgaca gcgaattcga
     1801 gtcggtctcg gcctcgctga aggttccgtt ctacaagact ttctggcgcg tcacggtgcc
     1861 ggtgtgcctg ccgtcgatcc tcgacatcgg tcgctactat ttcgtcaatg cgatgacgac
     1921 gatctcggcg gtggtgttcc tgtattcgcc gaagaccacg ctggcgtcga tctcgatcct
     1981 gcaactggac gaagcgggtg cgatcggctc agccgcggcg atggcgacgc tgatcgtggt
     2041 cacctccgca gtcgtgaccg gcgtactgtt tgccatcgag tggtggctga tccgacgtac
     2101 gcaatcctgg cgcaacacag cgctgcaata agtacaagga gaccccgatg attgtcggca
     2161 acgatccgat cctgctcacg cccgggccac tgacgacttc tctcgcgacc aaagcggcca
     2221 tgctgcgaga ctggggctcg tgggatgacg cattcaatgc gatcacggcg agcttgcggc
     2281 gcgacctggt cgacatcgtc agcggtagca gcgagtacac ctgcgttccg ctgcagggca
     2341 gcggcacgtt ttccgtcgag gcagccatcg gctcaatcgt gccgcgcgaa ggcaaggttc
     2401 tggtaccgaa caatggcgcc tattgcgaac gcatcgtccg gatctgcggt tacctaggcc
     2461 gggcggttgt cgatctgccg gtgcccgagg accagccggc gac
//
LOCUS       DATIRT010000210         2093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1209725, whole genome shotgun sequence.
ACCESSION   DATIRT010000210 DATIRT010000000
VERSION     DATIRT010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209725"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VLN25_04925"
     CDS             <1..537
                     /locus_tag="VLN25_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSQ09944.1"
                     /translation="PYVDEKTRLVPASRWIDFTGGRDVAFERGGIRFRLIDVGGAHSP
                     EDIMLWVENDRVLLAGDLYFSGRIPFVGNADTRSWLATLDVIAPLKPASVIPGHGAAS
                     RDPAPDIALTREYMLYLREQMGKAARDLVPFDEAYAAVDWSRFEKVPAFAAANRINAY
                     GTYLAMEQEALTPAAARK"
     gene            645..1301
                     /locus_tag="VLN25_04930"
     CDS             645..1301
                     /locus_tag="VLN25_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09945.1"
                     /translation="MLKRLSSVLIAAAAILVLGCATSSTTLDAQWVNPEYAGKRPIRS
                     MLVMAAVRDSTNRRISEDRMVAALSAVGVKAVQSYTLVPEDGPLTEERLRRMVTEAGV
                     SHAMVTRIINVSTQVNVSPGMVMGPAWGPGWGWGPAWGPGWGGMAGYHNAMWATSIPP
                     QVTTTQNVHADTRVFDAKSAAVLWSAATTTSTGYNSVQQLIEQFAQLIVATMTKDGVI
                     "
     gene            complement(1347..1871)
                     /locus_tag="VLN25_04935"
     CDS             complement(1347..1871)
                     /locus_tag="VLN25_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09946.1"
                     /translation="MKGNVSRSRELVLVPENRRDIDAVARLCRKLATRRALISAGASV
                     VPIPGIDIAVDIGVLMSMIEDINAAFGLTPDQIDRLAPRRKVFAYKAVMAVGSALVGR
                     VMTRELVIRVLKTVGVRLTAKQAARAVPIAGQVVAASLSFAALKYLGDRHIQDCVQVA
                     DTLLDMTGPGGSQK"
BASE COUNT          377 a          693 c          668 g          355 t
ORIGIN      
        1 ccctacgtgg acgaaaagac gcggctggtg cctgccagcc gttggatcga cttcactggc
       61 ggccgggatg tcgccttcga gcgcggcggc atccgctttc gcctgatcga cgtcggcggc
      121 gcgcattcgc cggaagacat catgctgtgg gtcgaaaacg accgcgtgct gctggctggc
      181 gacctgtact tcagcgggcg catccctttc gtcggcaatg ccgacacgcg ctcctggctc
      241 gccacgctgg atgtcatcgc gccactcaag cccgcgagcg tgattccggg ccacggtgcg
      301 gcctcgcgtg atccggcacc cgacatcgcg ctgacgcgcg aatacatgct gtacctgcgc
      361 gagcagatgg gcaaggcggc gcgcgatctg gtgccgtttg acgaggcgta tgccgcagtc
      421 gactggtcgc gcttcgagaa agtccctgcc ttcgccgcgg ccaatcgcat caacgcctac
      481 gggacctacc tggcaatgga gcaggaagcc ctcacgccag ctgccgcaag aaagtgaatg
      541 cgcggcagca ggtcggtcgg ctttccggag ccgaccggtg ccggttgcgc taacctgcgc
      601 gctgttggcg aaagcccatg ccctttcctc ttcggagcac gacgatgctg aagcgcttga
      661 gcagtgtcct aattgcggcg gccgcaattc ttgtcctcgg ctgcgcgact tcatcgacca
      721 cgctggacgc acagtgggtc aaccccgagt acgccggcaa acggccgatc cgcagcatgc
      781 tggtgatggc cgcggtccga gactcgacga atcgacgaat cagcgaagac cggatggtcg
      841 cggcgttgag tgccgtgggc gtcaaggccg tccagtccta cacactggtc ccggaagacg
      901 gtccgctgac agaggagcgt ctgcgtcgca tggtcaccga ggcgggtgtc tcgcatgcga
      961 tggtcacgcg catcatcaac gtctcgacac aggtcaatgt ctcgcccggc atggtcatgg
     1021 gtccggcctg gggtccggga tggggctggg gacccgcctg gggacccggc tggggcggca
     1081 tggccggcta tcacaacgcc atgtgggcaa cgtcgatccc gccgcaggtc acgacgacgc
     1141 agaatgtcca tgctgatacg cgcgtgttcg atgctaagag tgccgccgtg ctgtggtcgg
     1201 ccgccaccac gacgtccacc ggctacaact cggtgcagca gctgatcgag cagttcgcgc
     1261 aactgatcgt ggcgaccatg acaaaagatg gcgtgatctg aggaccgcgg cacggcaggt
     1321 attcgggccg gttttcccgg ctcgattcat ttctggctgc cgccggggcc ggtcatgtcg
     1381 agcagcgtgt cggccacctg cacgcagtcc tggatgtgtc ggtcgcccag atatttcaac
     1441 gctgcgaagc tcagcgatgc ggccaccacc tggcccgcga tcggaaccgc ccgtgctgcc
     1501 tgtttggcgg tcaatcgaac cccgacggtc ttcagcaccc tgatcaccag ttcacgagtc
     1561 atcacccggc ccacgagcgc gctgccgaca gccatcacgg ccttgtaggc aaacaccttg
     1621 cggcggggcg cgagccggtc gatctggtcg ggcgtcaagc cgaacgcggc attgatgtcc
     1681 tcgatcatcg acatcaacac gccgatgtcc accgcaatgt cgatgcctgg aatcgggacg
     1741 accgaggcac cggcggaaat cagggcacgc cgcgtcgcca gcttgcggca aagacgcgcc
     1801 acggcgtcga tgtcacgccg gttctccggc acgaggacca gttctcgcga tctgctcaca
     1861 ttgcccttca tgccccaatg ctaccgggcg tcgcccggag cacgcatgag agcctgacga
     1921 cgctccggcg caagccccga ggaacacact gccgacggag ggcttagcgc gtcgcagcaa
     1981 atggccgccg ctagacatgg atcaagtcgc cacccatccg gtatgtcgac aaaccactac
     2041 gacgcctatc gtgcgactgc cgacaagaac aagtgcgcag cgcggtccgc atc
//
LOCUS       DATIRT010000211         3280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1602868, whole genome shotgun sequence.
ACCESSION   DATIRT010000211 DATIRT010000000
VERSION     DATIRT010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602868"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VLN25_04940"
     CDS             <1..535
                     /locus_tag="VLN25_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HSQ09947.1"
                     /translation="IRTVCIIYVELVRGVPLISVLFMASFMFPLFMPQGFTIDVLVRV
                     LVGITLFAAAYMAEIVRGGLQAIPKGQVEAADSLGLTYWQTQRKIVLPQALAMVVPGI
                     MNNFISIFKDTSLVTIVSLYELTGALGIALNSDADWRPFKIEGYLFITLIYFILCFAM
                     SRYSLWVEKQLNRSKTR"
     gene            565..1317
                     /locus_tag="VLN25_04945"
     CDS             565..1317
                     /locus_tag="VLN25_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HSQ09948.1"
                     /translation="MTHPTHNIITFDKVNKWYGNNFHVLRDIDLHVKKGERIVICGPS
                     GSGKSTLIRCINRLEEYQEGKLAVDGIELTDDVKAIEKIRREVGMVFQQFNLFPHLTV
                     MENLTLAPMWVAGMPKAEAEERAMTQLNRVRIAEQAQKYPLQLSGGQQQRVAIARALC
                     LTPKVMLFDEPTSALDPEMIKEVLDVMIELAGQGITMLCVTHEMGFAKAVADRVIFMD
                     QGQIVEQNNPHEFFDNPKSERTKDFLSKILGH"
     gene            1426..2853
                     /gene="cls"
                     /locus_tag="VLN25_04950"
     CDS             1426..2853
                     /gene="cls"
                     /locus_tag="VLN25_04950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HSQ09949.1"
                     /translation="MPELSSLHWGTIAIVADWVIRLVMLPIVPTRRSPEAAKGWLLLI
                     FFLPWVGLVAYVLIGRPRLPRWRLERLQEFLHRVEPLRTRLVPPPQAAPPGVLVPYAA
                     SVRLSTDLAGMPNSGGNDVELMTDYAAILERIASDIESARVSVHACYYIFAEDARTAP
                     VIDALGRAAARGVRCRVLVDAFGSRPTIPSVVPRLRSLGIDARVALPFRWRKPERLDL
                     RNHRKIVVVDGRIGYTGSQNMVSPDFKPGLTYSELVARIEGPTALQLQLVFAADWYSE
                     SAEFIGEDAFPSPRIVGPCPAQVLPNGPASSTQRALRMVVNLVYAAQRRVCITTPYFI
                     PSQSLQEAMETAALRGVDVHLIVDRRKDQFMVANAQRSYYESLLVAGVHIHAYHEAFL
                     HTKAVSVDGEIAWVGSCNMDLRSFEINEEVIALFYSRDIASRLEGIEAGYMRGSEQVE
                     LDAWRRRPFRDQLVQNLTRLVSPLL"
     gene            2982..>3280
                     /locus_tag="VLN25_04955"
     CDS             2982..>3280
                     /locus_tag="VLN25_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09950.1"
                     /translation="MSAIESTAVPGAKSAVLPYAATIFLSAFLLFLVQPIIAKQILPW
                     FGGSSAVWTTALVFFQSTLLAGYAYADLTQRLGVRRQTLLHVGLLLLSLLTLPILA"
BASE COUNT          581 a         1035 c         1056 g          608 t
ORIGIN      
        1 gatccggacc gtctgcatca tctacgtcga gctggtgcgc ggcgtgccgc tgatctcggt
       61 gctgttcatg gcgagcttca tgtttccgct gttcatgccg cagggattca cgatcgacgt
      121 gctggtgcga gtgctggtgg gcatcacgct gttcgccgcg gcgtacatgg cggaaatcgt
      181 gcgcggcgga ctgcaggcga tcccgaaggg gcaggtcgaa gccgcggatt cgctcggact
      241 cacctactgg cagacgcagc ggaagatcgt gctgccgcag gcgctggcga tggtcgtgcc
      301 gggcatcatg aacaacttca tttcgatctt caaggacacg tcgctggtca ccatcgtctc
      361 gctctacgaa ctgactgggg cactcggtat cgcgctcaat tcggatgccg actggcgccc
      421 gttcaagatc gagggctacc tcttcatcac gctgatctac ttcattttat gcttcgcgat
      481 gtcacgctac agcctgtggg tggaaaagca gctcaaccgc agcaagaccc gatagccaac
      541 gagcgatagc caacgagcgc cgccatgacg caccccacgc acaacatcat cacgttcgac
      601 aaggtcaaca agtggtacgg caacaacttc cacgtgttgc gcgacatcga cctgcacgtg
      661 aagaagggcg aacggattgt catctgcggc ccgtcgggtt cgggcaagtc gacgctgata
      721 cgctgcatca accgcctcga ggagtaccag gagggcaagc ttgctgtcga cggcatcgag
      781 ctgaccgatg acgtcaaggc catcgagaag atccgccgcg aagtcggcat ggtgttccag
      841 cagttcaacc tgttccctca tctgacggtg atggagaacc tgacgctggc gccgatgtgg
      901 gtggcgggca tgccgaaggc cgaggctgag gaacgcgcga tgacacagct caaccgcgtg
      961 cgcatagcgg aacaggcgca gaagtatccg ctgcaactgt ccggggggca gcagcagcgg
     1021 gtggccattg cgcgggcgtt gtgcctgact ccgaaagtga tgctgttcga cgagccgacc
     1081 tcggcgcttg atccggagat gatcaaggaa gtgctcgacg tgatgatcga gctggctggc
     1141 cagggcatca cgatgctgtg cgtaacgcac gagatggggt ttgccaaggc ggtcgctgac
     1201 cgcgtgatct tcatggacca gggccagatc gtcgagcaga acaacccgca cgaattcttc
     1261 gacaacccga agagcgagcg gacgaaggac ttcctgtcga agatcctggg acattgagtg
     1321 acgaccccca cgctctctgc gttcgctgcc ccctgcgggg gcgctcagtc agcttgggag
     1381 cggcctggcg ccgactgaac gcgcggccca gcgccgcggt ccctgatgcc tgaactgtcc
     1441 tcgctgcact ggggaacgat cgccatcgtc gcagactggg tgattcgcct cgtcatgctg
     1501 ccgatcgtac cgacgcggcg ttcgcccgag gcggccaagg gctggttact gctgatcttc
     1561 ttcctgccgt gggtcgggct ggtggcgtac gtcttgatcg ggcgaccgcg actgccgcgc
     1621 tggcggctcg agcgcctgca ggaattcctg catcgggtcg aaccgctgcg cacgcgcctc
     1681 gtaccgccgc cgcaagccgc accgccggga gtactcgtgc cctacgccgc cagcgtccgg
     1741 ctgtcgaccg acctggcggg aatgccgaac tccggcggca acgacgtcga gctgatgacc
     1801 gactacgcgg ccatcctcga gcgcatcgcg tccgacatcg aaagcgcgcg cgtgtccgtg
     1861 catgcgtgct actacatctt cgcggaagat gcacgcaccg caccggtgat cgacgcgctc
     1921 ggtcgcgcgg ccgcacgtgg agtgcgttgc cgggtgctgg tggacgcgtt cggttcgcgc
     1981 cccacgatcc cgtcagtcgt tccgcgactg cgcagcctgg gaatcgacgc cagggttgcg
     2041 cttccgtttc gctggcgcaa gcccgagcgg ctcgacctgc gcaaccaccg caagatcgtc
     2101 gtcgtcgacg ggcgtatcgg ctacacggga tcacagaaca tggtcagtcc ggatttcaag
     2161 cccggactga cctacagcga actggtggcg cgcatcgagg gcccgacggc gctgcaattg
     2221 cagttggtct tcgcggccga ctggtattcg gagtcggccg agttcatcgg cgaggacgcg
     2281 ttcccttcgc cgcggatcgt cgggccttgc ccggcgcagg tcctgcctaa cgggccagcg
     2341 tcatcgacgc agcgcgcgct gcgcatggtg gtgaacctgg tctacgccgc gcagcggcgc
     2401 gtgtgcatca cgacgccgta tttcatcccg agccagtcac tgcaggaagc gatggaaacg
     2461 gcggccctgc gcggcgtcga cgtgcacctg atcgtcgatc gccgcaagga ccagttcatg
     2521 gtcgccaatg cgcagcgctc gtactacgag tcgctgctgg tcgccggagt gcacatccat
     2581 gcctaccacg aggcgttcct gcacaccaag gccgtttcgg tcgacggcga gattgcgtgg
     2641 gtcgggtcct gcaatatgga cctgcgctcg ttcgagatca acgaggaagt gattgcgctt
     2701 ttctacagcc gcgacattgc atcgcgactc gaaggaatcg aagccggata catgcgcggc
     2761 tccgaacagg tggagctcga cgcctggcgt cgccgcccgt tccgcgatca gctcgtgcag
     2821 aacctgacgc gactggtcag tccactgctg taagtcaagg cagccgactt catccggtaa
     2881 gcctggagca cgcacagcct gcggccggta ctcaggcgct gctgcccaac ttccgcaatc
     2941 catgataatc gcggctttgc aggacgcggc gaaacccatt catgtcagcc atagagtcca
     3001 ccgcagtgcc gggtgcgaag agtgcggtgc tgccgtatgc ggcgacgatc tttctatccg
     3061 cgttcctgct gttcctggta cagcccatca ttgcgaagca gatcctgcca tggttcggcg
     3121 ggtcgtcggc cgtgtggacc acggcgctgg tgttcttcca gtcgacactg ctggccggtt
     3181 acgcctacgc ggacctgaca caacggctgg gcgtgcggcg ccagacgctg ctgcacgtcg
     3241 gattgctgct gctgtcgctg ctgacgctgc cgatcctggc
//
LOCUS       DATIRT010000212         1679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1307996, whole genome shotgun sequence.
ACCESSION   DATIRT010000212 DATIRT010000000
VERSION     DATIRT010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307996"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VLN25_04960"
     CDS             complement(<1..688)
                     /locus_tag="VLN25_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HSQ09951.1"
                     /translation="MNQSTTWERLRTNRDWLGFWFMLPTAAFLLLFLVYPLGLGIWLS
                     FTDARIGTPGKFIGLENYEWIIGDSKFQTAVFFTLLYTLVASAFKFAIGLYLALLLNQ
                     SMPFKAMVRAVILIPFIVPTVLSAIAFWWIFDPQFSVVSWSLRQLGWIEGNINFLGDA
                     WNARWSVIFANVWRGVPFIAISLLAGLQTLPPSLYEAATLDGATRWQLFRHVTYPLLT
                     PIIAVVMTFSV"
     gene            complement(710..>1679)
                     /locus_tag="VLN25_04965"
     CDS             complement(710..>1679)
                     /locus_tag="VLN25_04965"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HSQ09952.1"
                     /translation="DVTDVADYLGKKYGGWVASAVTYGKSGKGWIAIPVGYNGNVINY
                     RQSMIEKAGFKEVPKDTAAFMELMKALKAKSVAGGFALGRASGDGNAWVHWCLWAFGG
                     NLVDDKDKVILNSPETVKALEYAKEMSTHFVPGCAAWNDSFNNKAFLESQISLTNNGV
                     SIYAAAKANAAKDPKAKEVMDDMNHSLWPVGPVGKPTEFHVAYPMLGMRYTKFPQATK
                     AFLAFTLEAENYNPWLQAAVAYITHPLNAYDANPVWTSDPKLSVARDCAKRTLTAGGL
                     GSVGEKAASALADFVILDMFASVCTDRASIKDAISVAERQARRIYR"
BASE COUNT          309 a          536 c          538 g          296 t
ORIGIN      
        1 gcaccgagaa ggtcatgacc accgcgatga tcggcgtcag cagcggatag gtgacgtgcc
       61 ggaacagctg ccagcgcgtc gcgccgtcga gcgtcgcggc ttcgtaaagc gacggcggca
      121 gggtctgcag cccggcgagc agcgatatgg cgatgaaggg caccccacgc cagacgttgg
      181 cgaagatgac gctccagcgc gcgttccacg catcgccgag gaaattgatg ttgccctcga
      241 tccaccccag ctgccgcagg gaccaggaga cgaccgagaa ctgcggatcg aagatccacc
      301 agaaggcgat ggccgacagc acggtcggca cgatgaaggg tatcaggatt accgcccgga
      361 ccatcgcctt gaacggcatg ctctggttca gcagcagcgc aaggtacagg ccgatggcga
      421 acttgaatgc gctcgccacc agcgtataga gcagcgtgaa gaacaccgcg gtctggaact
      481 tggagtcgcc gatgatccac tcgtagttct ccaggccgat gaacttgccc ggcgtgccga
      541 tgcgcgcgtc ggtgaacgag agccagattc ccaggccgag cgggtaaacc aggaacagca
      601 gaaggaacgc cgccgtgggc agcatgaacc agaacccgag ccagtcgcgg ttggtgcgca
      661 gccgctccca tgtcgtggac tggttcatgt ctttcccatc agcaccagct tagcgataga
      721 tccggcgcgc ctgccgttcc gccaccgaga tcgcgtcctt gatgctcgcg cggtcggtgc
      781 agaccgaggc gaacatgtcg aggatcacaa agtcggccag cgccgaagcg gccttttcgc
      841 ccaccgagcc caggccgccc gccgtgagcg tgcgcttggc gcagtcgcgc gccaccgaca
      901 gcttcgggtc ggaggtccac accgggttgg cgtcgtaggc gttcagcggg tgggtgatgt
      961 aagccaccgc cgcctgcagc cacgggttgt agttctccgc ttccagcgta aaggcaagga
     1021 acgccttggt ggcctggggg aacttggtgt agcgcatgcc gagcatcgga taggcgacgt
     1081 ggaactcggt cggcttgccc accggcccaa ccggccacag cgaatggttc atgtcgtcca
     1141 tgacctcctt cgccttggga tccttcgccg cgttcgcctt ggcggccgcg tagatcgaca
     1201 ccccgttgtt ggtgagcgag atctggctct cgaggaaggc cttgttgttg aaggaatcgt
     1261 tccacgccgc gcagccgggc acgaagtgcg tcgacatctc cttcgcgtac tccagcgcct
     1321 tgacggtctc gggcgaattg aggatcacct tgtccttgtc gtcgaccagg ttgccgccga
     1381 aggcccacag gcaccagtgc acccaggcat tgccatcgcc cgaggcgcgg ccgagcgcga
     1441 acccgcccgc gacgctcttc gccttgagcg ctttcatcag ttccatgaat gccgcggtgt
     1501 ccttcgggac ttccttgaag cccgccttct cgatcatgct ctggcggtag ttgatgacgt
     1561 tgccgttgta gccgacagga atggcgatcc aacccttgcc cgacttgccg taggtgacgg
     1621 cgcttgccac ccagccgccg tatttcttgc caaggtagtc ggcgacgtcg gtgacgtcc
//
LOCUS       DATIRT010000213         4360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_127912, whole genome shotgun sequence.
ACCESSION   DATIRT010000213 DATIRT010000000
VERSION     DATIRT010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127912"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..2674)
                     /gene="mdeB"
                     /locus_tag="VLN25_04970"
                     /pseudo
     CDS             complement(2..2674)
                     /gene="mdeB"
                     /locus_tag="VLN25_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011400980.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate dehydrogenase"
     gene            2779..3828
                     /gene="pyrC"
                     /locus_tag="VLN25_04975"
     CDS             2779..3828
                     /gene="pyrC"
                     /locus_tag="VLN25_04975"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HSQ09953.1"
                     /translation="MDSLTIARPDDWHLHLRDGAALAAVLPATAHQFARAIVMPNLKP
                     PVATTAQAQAYRQRILAALPPGLEFEPLMTLYLTDNMPADEIRRAVDSGIIHGVKLYP
                     AGATTNSDSGVTDIRKPRAALEAMQELGLPLQVHGEVTHPSVDVFDREKVFIDDVMEP
                     LRRDFPALKVVFEHITTRDAAHYVRDADGPLAATITAHHLLFNRNALFISADGRAGVR
                     PHYYCLPVLKRESHRLALVEAAVSGSPRYFLGTDSAPHPKGEKETACGCAGCYTAACA
                     LELYAEAFEDAGQLDRLEGFASHYGADFYGLPRNRGTVTLRRRAWNVPAELPLGDQKV
                     VPLRAGERLRLQLTN"
     gene            3891..>4360
                     /locus_tag="VLN25_04980"
     CDS             3891..>4360
                     /locus_tag="VLN25_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3025 domain-containing protein"
                     /protein_id="HSQ09954.1"
                     /translation="MAEGPDELIAALNRQLEERGITTTAGTRIFFAAPDDAPPGCAYE
                     SHIALTGRVPTRSNRHDLFNALVWLVFPRTKARLNALQATAIERAGVGAIRGPLRDAA
                     TVFDENGVVLVTRDECVIDDLRARRWNDVFLARRVAWGDVGVWIFGHALLDKLVA"
BASE COUNT          701 a         1442 c         1451 g          766 t
ORIGIN      
        1 gtcaccgtgc ccagggagga gcgctggcct gcaggcccca ggccgatcgt gctcgcgccg
       61 cggcggcggc atcgatcgca tcgagagcgg ccagtgcaat cgagcgacga tcgacttcga
      121 agaatgcacg cagggccgcc cgcgtatcgg agcgaccgaa gccatcggtg ccgagcgcga
      181 catagtgatc gccgatccat ggccggatca ggtcgggcac gctgctgacg taatccgacg
      241 ccgccaccac cggtgtcccg ttgcgcggaa gctgttgttc aacccaggac tttgcgggcg
      301 gcgcccccag ccttgcggcg cgctcgaggg ccatcgcttc gcgccgcagt tcggtgaagc
      361 tggtgaccga ataaacgtcc gctgcgatgc cgtagtcggt ctcaagcaat tcagccgcgg
      421 ccagcgcctc gcgcaggatc gtgcccgcgc cgagcagccg caccctcgcg gcaccctttg
      481 gcgagggccg aacgcagtac atgcccctca ggatgccgtt tcgtgacgtg tccagcgcgg
      541 acggctgtgc gtagttttcg ttcatcaccg tcacgtaata gaactcgtcg gcctgctcgc
      601 tcatcatgcg tcgcatgccg tgatcgacga tcacggccag ctcgtacgcg tagcacgggt
      661 cgtaggcgcg gcagttcggc acggtcgagg cgatcaggtg actggttccg tcctcgtgtt
      721 gcagtccttc gcccgacagc gtcgtgcggc cggcggtcgc accgatcagg aaaccgcgcg
      781 tgcgtgagtc cgcggcggcc cacagcagat cgccgacgcg ctggaagcca aacatcgagt
      841 agaagatgta cagcggcagc atcgccagac cgttggtcga gtagctcgtg cccgccgcca
      901 cccatgatgc gatcgcgccg gcctcgttga tcccttcctc gaggatctgt ccggacttcg
      961 attccttgta gtagctgatc tgggcgctgt cctcgggctc gtagagctgt cccagcggcg
     1021 cgtagatgcc gatctgccgg aacaggtccg ccatgccgaa ggtgcgcgct tcatcggcga
     1081 tgatcgggac gacccgcggc ccgacggcgg cgtccttcag cagtcccccg agcatgcgca
     1141 ccagcgccat ggtcgtcgac atttccttgc cgctggcctc gatcgcgaac cgcgcgtagg
     1201 tctcgaccgg tggcgtcgcc acgggctcgc aggccgtgga ccgtgccggc atgtagccgc
     1261 cgagcctggc acggcgtgcg tggaggtact tcatctccgg gttgtcatcg gccggcttgc
     1321 agaacgcgac gttttcgacc tgcgcatcgc tgagcggcag cgcgaagcga tcgcggaact
     1381 cgcgcagcgc ctcggcgtcg agttttttct gctggtgcgt cgtcattcga ccctgtccgg
     1441 ccgtgcccat gccgtagccc ttcttcgtct tggcgaggat cactgtcggc tgcccccggt
     1501 ggcgcgcggc ggcgaagtag gccgcgtgga tctttaccgg atcgtgccct ccgcgtcgca
     1561 gtcggtcgat atcgtcgtcg gacaggtgcg cggtcaactg cgccagttcg gggaacttgt
     1621 ggaagaagtg ctcgcgattg aaacgtccgt cggtcgccga cagagtctgg aactcgccgt
     1681 cgaccgtctc gtggaaggcg cgcaacagca gcatctgcgt gtcgcgtgca aagagcgggt
     1741 cccagtcgct gccccacagc agcttgatca cgttccagcc cgcgccggca aagagccctt
     1801 cgagttcctg gatgatcgag ccgttgccgc gtaccggccc gtcgagccgc tgcaagttgc
     1861 agttgacgac gaacaccagg ttgtcgagcc cttcgcgtgc agcgatgctc agtcccgcga
     1921 gtgactcggg ttcgtccatt tcgccgtcgc cgacgaaagc ccagaccttg cgcgcgtagg
     1981 tcgatgccag tccgcgattc tcgatgtagc gcaggaagcg cgcctggtag atcgcgaaga
     2041 gcggccccag tcccatctag cccgtcggga actgccagaa gttcggcatc aaccacggat
     2101 gcggatacga actcaggccg tggcctgcgg tttcgcgtcg atagtggtcg agctgatccg
     2161 ccgtcagccg gccttcgaga aatgcgcggg cgtagacgcc gggcgccgaa tgcggctggt
     2221 agtacaccag gtcaccgcgt ccaccggcgc cgtcggcacg gaagaagtga ttgaagccga
     2281 cttcgaacag atctgctgcc gacgcatagc tggcgatgtg tccaccgagt tccggactcg
     2341 cctggttcgc acgcacgacc atcgccagcg cattccagcg caccagtgcc gacaggcgct
     2401 gctcgatctc gaggttgccg ggaaacggcg cctgttcgcc gagcgaaatc gtgttcaggt
     2461 agggcgtgtt caacacctgc ggcaacggca cgcggcgcgt acgcgcgtgc tgcagcaact
     2521 gcttaagcac gaaggttgcg cgtgctggcc cctcggccgc gatcaacgcg tcgagcgctt
     2581 cgagccattc gcgcgtttcc tgcggatcgc cgtcatcctc gggcagccgc cacgcagcgt
     2641 tgggaatcag tttcgcgagg tcgtcgaggt tcatcggcgc tcgggatcgg gagggtcggc
     2701 cattatcacc ctcgactggg cgccggtgct gcggatcggg gaatccgctg ccgggcccag
     2761 ggttgcgata atcacggcat ggactctctg acgattgccc gcccggacga ctggcacttg
     2821 cacctgcgtg acggcgcggc actggccgcc gtgctgccgg cgaccgcgca ccagttcgcg
     2881 cgggccatcg tgatgccgaa cctgaagcct ccggtggcga cgacggcgca ggcgcaggcg
     2941 taccggcagc gcatccttgc cgctttgccg ccggggctcg agttcgagcc cctgatgacg
     3001 ctatacctga ccgacaacat gcctgcggac gagatccggc gggccgtcga cagcggcatc
     3061 atccacggcg tcaagctgta ccccgcgggg gcgaccacga actcggactc gggcgtcacc
     3121 gacatccgca agccgcgtgc cgcgttggaa gccatgcagg aactcgggct gccgctgcag
     3181 gtccacggcg aggtcacgca cccaagcgtc gacgtcttcg atcgcgagaa ggtcttcatc
     3241 gacgatgtga tggagccgct gcggcgcgac tttccggcat tgaaggtcgt gttcgagcac
     3301 atcaccacgc gcgacgcggc gcactacgtg cgcgatgcgg acggtccgct ggccgcgacg
     3361 atcaccgcgc atcatctgct cttcaaccgc aatgcgctgt tcatatcggc ggacggccgt
     3421 gccggcgtgc ggccgcacta ctactgtctg ccggtgctca agcgcgaatc gcaccggctg
     3481 gcgctggttg aagccgcggt cagcggcagc ccccgctact tcctcggtac cgattcggcg
     3541 ccccatccga agggcgagaa ggaaaccgcc tgtggctgcg ccggctgcta cacggccgcc
     3601 tgtgcgctcg agctctatgc cgaggcgttc gaggatgccg gacagctcga tcgactggaa
     3661 ggattcgcga gccactacgg agccgacttc tacggactgc cgcgcaatcg cggcaccgtg
     3721 acgctgcggc ggcgcgcttg gaacgtgcct gccgagcttc cgctcggtga tcaaaaggtc
     3781 gtgccgttgc gcgcgggcga acgtctgcgg ttgcaactca cgaactgagt gcgatcgact
     3841 ggtcgcagcc cggcttcgcg gcgattgccg atgtcggtcg ctgcgtatcc gtggccgagg
     3901 gcccggatga actgatcgcg gcactcaatc gacagctcga ggagcgcggc atcacgacaa
     3961 cggcgggaac ccggatcttc ttcgcagcgc ctgacgatgc gccgcccggc tgcgcctatg
     4021 agtcccacat cgcgctgacg ggaagagtgc cgacgcgttc gaaccgccat gacctcttca
     4081 atgcgctcgt ctggctcgtg tttccacgga ctaaggcgcg cctgaatgcc ttgcaggcca
     4141 cggcgatcga acgcgcggga gtgggtgcca tccgcgggcc gctgcgcgat gcggccaccg
     4201 tgttcgacga aaacggcgtc gtgctggtca cccgggacga gtgcgtcatc gatgatctgc
     4261 gggcacgacg ctggaatgac gtctttttgg cgcgccgtgt ggcatggggt gacgtcggcg
     4321 tgtggatctt cggccacgct ctgctggaca agctggttgc
//
LOCUS       DATIRT010000214         7982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1112798, whole genome shotgun sequence.
ACCESSION   DATIRT010000214 DATIRT010000000
VERSION     DATIRT010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7982
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112798"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VLN25_04985"
     CDS             <1..206
                     /locus_tag="VLN25_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09955.1"
                     /translation="ELLAGIHIGDEVDGRMRYIDQKGAVFYVHPNEVEKRQPSSQSIM
                     PEGLVDQLTQQELRDLMAFLLRL"
     gene            complement(296..685)
                     /locus_tag="VLN25_04990"
     CDS             complement(296..685)
                     /locus_tag="VLN25_04990"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HSQ09956.1"
                     /translation="MVWAQNPVVDQAAAEKGAIKTDSGMVYRSLKDGSGASPKETDVV
                     SVHYRGTLPDGKEFDSSIKRGQPAEFPLNRVIKCWTEGVQKMKVGGKAKLTCPASVAY
                     GERGTPGGPIPPNAVLIFEVELLDIVK"
     gene            815..2143
                     /locus_tag="VLN25_04995"
     CDS             815..2143
                     /locus_tag="VLN25_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HSQ09957.1"
                     /translation="MQDLFTTDAASVPLAERMRPRSIDEVVGQSHLLGPGKPLRVAFE
                     SARPHSMILWGPPGVGKTTLARLMADAFGLDFIAISAVLGGVKDIREAVERAQGTRAR
                     AGRPTVVFVDEVHRFNKGQQDAFLPHVESGLFVFVGATTENPSFEVVGALLSRAAVYV
                     LEPLNAEELETLITRAASGALGGVEVDATARRMLIDLADGDARRLLNALEIAAGAARD
                     AGASTVDADFLRAALPATLRRFDKGGENFYDQISALHKSVRGSDPDASLYWMTRMLDG
                     GADPRYIARRVVRMAVEDIGLADPRGLEIALAAAQTYERLGSPEGELAIAQAVVYLAV
                     APKSNALYAAYNAARAFVQGDASRPVPMHLRNAPTRLMKDLGYGAGYRYAHDEPEGYA
                     AGERYFPDGLTSLRFYEPSEQGVEARIRERLAQLRSRDAKRKDADPSDGS"
     gene            2400..3704
                     /gene="serS"
                     /locus_tag="VLN25_05000"
     CDS             2400..3704
                     /gene="serS"
                     /locus_tag="VLN25_05000"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HSQ09958.1"
                     /translation="MLDINLLRKDIGAAAERLRTRGFDFDIDGFNVLEAERKTVQSST
                     EELQARRNSLSKQIGVLKSKGEDASAVLAEVGAIPEQVKDLEIRLAGIQARLHDMLLN
                     FPNLPHASVPVGRSADDNPEVRRWGIPREFDFAPRDHVDVGAALGLDFEAAAKLSGSR
                     FAVLRGPVARLHRALAQFMLDTQTGEHGYTECYTPYIVNRETLLGTGQLPKFRDDMFW
                     VTKGGGERGDGEGKEELYLISTSEISLTNTVRGDVLREADLPIRLTAHTPCFRSEAGS
                     YGKDTRGMIRQHQFDKVEMVQITHPERSYEVLEQMVGHAEAILQKLGLPYRVVALCTG
                     DLGFGSTKTYDLEVWLPGQSAYREISSVSNCEAFQARRMQTRFKNAQGKTELVHTLNG
                     SGLAVGRTLVAVLENFQQADGSVKVPEVLRPYMQGLEVLKPV"
     gene            3755..3845
                     /locus_tag="VLN25_05005"
     tRNA            3755..3845
                     /locus_tag="VLN25_05005"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3789..3791,aa:Ser,seq:cga)
     gene            4348..6330
                     /gene="acs"
                     /locus_tag="VLN25_05010"
     CDS             4348..6330
                     /gene="acs"
                     /locus_tag="VLN25_05010"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HSQ09959.1"
                     /translation="MSNIESVLQEHRVFPPATEFIKQANVSGMGGYQALCAEAEKDFT
                     GFWGRLARENLSWHKPFTKVLDESNAPFYQWFPDGELNASYNCLDRNLSNGNADKVAI
                     IFEADDGTITKVTYRDLHKEVCRLANALKQHGVKQSDRVLIYMPMSVQVVVAMQACAR
                     IGATHSVVFGGFSAKSLQERIIDAGATEVITADGQFRGGKEIPLKPVVDEALAMGGCE
                     GVRNVIVYRRTGSNVPMQAPRDKWWDDVVKGQPETCEPTWVNAEHPLFILYTSGSTGK
                     PKGVQHSTGGYLLWAVLTMKWTFDYKPSDVFWCTADVGWVTGHTYITYGPLAVGATEV
                     MFEGIPTYPDAGRFWKMIQDHKVSVFYTAPTAIRSLIKANEGNPATHPTKYNLSGLRI
                     LGTVGEPINPEAWMWYHTNVGGGRCPIVDTWWQTETGGHLITPLPGVTPTVPGSCTLP
                     LPGIMAAIVDETGHDVPNGQGGLLVIKRPWPAMIRTIWGDPERYKKSYFPEELGGTLY
                     LAGDGSIRDKETTYFKIVGRIDDVLNVSGHRMGTMEIESALVSNPMVAEAAVVGRPDD
                     MTGEAISAFVVLKRARPTGEEAQQIAKELRDWVGKEIGPIAKPKDIRFGDNLPKTRSG
                     KIMRRLLRSIAKGEEITQDISTLENPAILDQLKVTL"
     gene            6646..6990
                     /locus_tag="VLN25_05015"
     CDS             6646..6990
                     /locus_tag="VLN25_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09960.1"
                     /translation="MAMAFALLALQTLVLVAGLALLGQGLVGAFNWSRRRQNLVWRLF
                     EVIASPVVKVVRLVTPKFVLDQHIPIAAFMLLFFAYFWLGFEHRSTCRADTAQAGCEK
                     WAAAWSGATVKQ"
     gene            6963..7628
                     /locus_tag="VLN25_05020"
     CDS             6963..7628
                     /locus_tag="VLN25_05020"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSQ09961.1"
                     /translation="MERRNGEAMTWLAQKLKLWWLQRQANAATLFKRVPDAVRAYRDM
                     LAIDPGNQMAGLMLGNLLAEAGDREGAVAQLEQLTVVNPSFADAWFNLGYLHDQADRL
                     TDAERCFRRAVELRPSLDRAWYGLGLVLIRDGRLTEAVDALKQTIKLQPFSPYGYYQL
                     GLTYHHLGRNEDAWRVHKQLTEFEPKFSATLKRDLERTVPQAVPAINPNSQPKEETTV
                     DAT"
     gene            7662..7928
                     /locus_tag="VLN25_05025"
     CDS             7662..7928
                     /locus_tag="VLN25_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HSQ09962.1"
                     /translation="MQLTEKHKEYWSRNLRITAILLFIWFVVTYVMAYFAIPLAKINF
                     FGWPLSFYMAAQGSLIIYVLLIWYYAKYMRKLDLEYGVDEGDLK"
BASE COUNT         1547 a         2425 c         2585 g         1425 t
ORIGIN      
        1 gtgaattgct ggccggcatt cacatcggcg acgaggtcga tgggcgcatg cgatatatcg
       61 atcagaaagg cgcggtgttt tatgtacatc cgaacgaggt tgagaagcgg cagccgagca
      121 gccaatcgat catgcccgag ggcctggtgg atcaattgac acagcaggag ctgagggacc
      181 ttatggcgtt cttgcttcga ctttagccgg gagcaaactt gctcgcggag aaccgccgac
      241 cgacccgttt tgctgaggct ccgggcccag gtttggtccc ggctaggtgc agcgattact
      301 tcacaatatc cagcagctcc acttcaaaga tcagcaccgc attcggcggg atcggtccgc
      361 cgggcgtgcc gcgttcgccg taggcgacac tggcagggca ggtgagcttg gccttgccgc
      421 cgaccttcat tttctgcacg ccttcggtcc agcacttgat cacgcgattg agcggaaatt
      481 ccgccggctg gccgcgcttg atcgagctgt cgaattcctt gccgtcgggc agcgtgccgc
      541 ggtaatgaac gctcacgaca tcggtttcct tcggactcgc accgctgcca tccttcaacg
      601 agcggtaaac catgccggag tccgtcttga tggcgccttt ttcggctgcg gcctgatcga
      661 ccaccgggtt ttgcgcccag accatcgggc ttgcgaaaag cgcaagcagc atccatttct
      721 tcatcgttgt ctactccgtt gcgttatgcc cgtgcgccag gtgctcgctg ggcggcgggc
      781 gcgatgctag cctgtctttg ctccggaccc aaacatgcag gatctgttca ccaccgacgc
      841 cgcctcagtg ccactggcgg aacgcatgcg accacgctcg atcgacgagg tcgtaggcca
      901 gtcacacctg ctcggtcccg gcaagccgct gcgggtcgcg ttcgagtcgg cgcggccgca
      961 ctcgatgatc ctgtggggcc cgccgggcgt gggtaagacc accctcgcga ggctgatggc
     1021 cgatgccttc ggcctcgact tcattgcaat ctcggcggtg ctcgggggcg tcaaggacat
     1081 ccgcgaagcg gtggagcgcg cgcagggcac ccgcgcgcgc gccggccggc cgacggtcgt
     1141 cttcgtcgat gaagtacatc gcttcaacaa gggccagcag gacgcgttcc ttccgcatgt
     1201 cgagtcgggc ctgttcgtct tcgtcggtgc gacgacggag aacccgtcct tcgaagtggt
     1261 cggcgcactg ctttcgcgcg ccgcggtcta cgtcctcgag ccattgaacg cggaggagct
     1321 cgaaacgctg atcacgcgtg ccgcgtccgg ggcgcttggc ggagtcgagg tcgacgcaac
     1381 ggcgcgtcgc atgctgattg atctggccga cggcgacgca cggcggttgc tgaatgcgct
     1441 cgaaatcgcg gccggtgcgg cacgggacgc gggcgcctcg acggtcgacg ccgactttct
     1501 gcgcgccgcc ttgccggcga cgctgcgtcg gttcgacaag ggcggggaga atttctacga
     1561 ccagatctcg gccctgcaca agtcggtgcg cggctccgac ccggatgcgt cgctctactg
     1621 gatgacgcgc atgctggacg gtggggcgga tccccgctac atcgcccggc gcgtcgtgcg
     1681 gatggcggtc gaggacatcg ggctggcgga cccacgcgga ctcgagattg cgctggcggc
     1741 cgcccagacc tacgagcgac tcgggtcgcc ggaaggcgaa ctcgccatcg cgcaggccgt
     1801 ggtctacctc gctgtcgcgc caaagtccaa cgcgctctac gcggcatata acgcagcgcg
     1861 cgccttcgtc cagggcgatg cgtctcgtcc ggtgccgatg catctgcgca atgcgccgac
     1921 ccgcctgatg aaggaccttg gatacggcgc cggctaccga tatgcgcacg acgaacctga
     1981 aggctatgcc gccggagagc gttattttcc cgatggcctg acttcgctgc ggttctacga
     2041 gcccagcgaa cagggggtgg aggcgcgcat acgcgaacgg ctggcgcaac tgcggtcgcg
     2101 cgatgccaag cgcaaggacg cggacccgag tgatgggtcc tagcctgcca accggcacga
     2161 cggtcggcag ggtcgggtcg gaaggggctt gcagggccgc aggccggcac tcgcggttgc
     2221 gcacgtcggc acgctcggcg agaatgcgcc catgcgctgc cacaccatcc cgcccagtcg
     2281 atcctgctcc cttgccagtg cccgcgctcg cgggtaattg gtacctctgc cgcgccgctg
     2341 cgcggccgct taaaaaacat tgacacgtcg gcgctgccga gacgctttca ggaactgcca
     2401 tgctggatat caacctgcta cgcaaggaca tcggtgccgc cgcggagcga ctacggacgc
     2461 gcggcttcga cttcgacatc gacggattca atgtgctcga agcggagcgc aagacggtgc
     2521 agagcagcac cgaggaattg caggcgcgtc gcaattcgct gtcgaagcag atcggagtgc
     2581 tgaagtcgaa gggcgaggat gcttcggctg tcctggccga agtcggggcg attcccgagc
     2641 aggtgaagga cctcgaaatc cggctggccg gcatccaggc gcggctgcac gacatgctgc
     2701 tcaacttccc gaacctgccg cacgcatcgg ttccggtcgg cagatccgcc gacgacaatc
     2761 cggaagtgcg ccgttgggga ataccacgcg agttcgactt tgcgccacgc gatcatgtcg
     2821 acgtgggtgc ggcactcggg ctcgacttcg aagcagcggc caagctgtcg ggatcgcgat
     2881 ttgcggtgct gcgaggaccc gtcgcccgcc tgcatcgcgc gctcgcgcag ttcatgctcg
     2941 acacgcaaac cggtgagcat ggctacaccg agtgctacac gccgtacatc gtcaaccgcg
     3001 agaccttgct agggactggt cagctgccga agttccgcga tgacatgttc tgggtcacga
     3061 agggaggcgg ggagcggggt gacggagaag gcaaggaaga gctctacctg atctccacct
     3121 cggaaatttc gctgaccaat accgtgcgcg gtgatgtgct gcgcgaggcg gacctgccga
     3181 tccggctgac cgcgcacacg ccgtgcttcc gctccgaggc aggcagctac ggcaaggaca
     3241 cgcgcggaat gatcaggcaa caccagttcg acaaagtcga gatggttcag atcacgcatc
     3301 ccgaacgttc gtacgaagtc ctcgagcaga tggtcgggca cgccgaagcc atactgcaga
     3361 aactcgggct gccgtaccgg gtggtggcgc tgtgcaccgg tgaccttggg ttcggctcga
     3421 cgaagaccta cgacctcgaa gtctggttgc cgggacagag cgcctatcgc gagatctcgt
     3481 cggtttcgaa ctgcgaggcg tttcaggcgc gcaggatgca gacccgcttc aagaatgccc
     3541 agggcaagac cgagctggtc cacacgttga atggctcggg cctcgcagtc ggtcgaacac
     3601 tcgtggcggt cctcgagaac ttccagcagg ctgatggcag cgtgaaggta ccggaggtgc
     3661 tgcgtccgta catgcaaggg ctggaggtcc tgaagccggt ctgacgtgcg tgcgcagata
     3721 taatgcgcgg ctcacttcag tgacgaccaa agccggagag gtggcagagt ggtcgaatgc
     3781 gccggattcg aaatccggtg tacggttcta ccgtaccgtg ggttcgaatc ccaccctcgc
     3841 cgccacagca aacgggcccg cgagaggccc gtttgctttg gcggagaggg tgtaagtaga
     3901 acccacacgg gttcgacgag caccgagtag cggcgcgaag gacaccggag cgcaacgacg
     3961 gtggtccgag gcacaagccg aggacgaggc agctctgctg ccgagcaatc ccaccctctt
     4021 caccatcgcg agttgtccgc attggcccct ccgctttggc gtgcacgagt gaaagagaac
     4081 acccgcctgc tcgacgatcg gtgcgatgca gcgagcctct ttgctagctc aggcggatgc
     4141 catccgaggc acgcagttgt cgagaattgg caattgctta cagccttctt acttcaacag
     4201 tgctctgctt ctacgaatag tgcattgcag caccaatttt gggcgtcctg tcagggttgt
     4261 gtaagcgcga ctacctaccg tccgcgcggg taatacaaat gacctagtca gaagaagaac
     4321 aagacacgcg ccgcggagac gggagacatg agcaacatcg aatcagtcct gcaagagcac
     4381 cgggtgtttc cgcccgcgac tgaattcatc aagcaggcca acgtgtccgg catgggtggg
     4441 taccaggcgc tatgtgccga ggcggagaag gacttcaccg gattctgggg caggctggcg
     4501 cgcgaaaacc tcagctggca caagccattt accaaggtcc tcgacgaatc gaacgcgccg
     4561 ttttaccaat ggttccccga cggcgagttg aacgcgtcgt acaactgcct cgatcgcaat
     4621 ttgagcaacg gcaatgccga caaggttgca atcatcttcg aggccgacga cggcaccatc
     4681 acgaaagtca cttaccgcga tctgcacaag gaagtgtgcc ggcttgcgaa cgcgctgaag
     4741 cagcacggtg tcaagcagag tgaccgggtt cttatttaca tgccgatgtc ggtccaggtc
     4801 gtcgtcgcga tgcaggcctg cgcgcggatt ggggcgacgc attcggtcgt ctttggcggc
     4861 ttctcggcga agagcctgca ggaacggatc atcgacgccg gcgccaccga ggtcatcacc
     4921 gcggacggcc agtttcgcgg cggcaaggaa atcccgctga agccggtggt ggatgaagca
     4981 ttggcgatgg gtggctgcga gggtgttcgc aacgtcatcg tctacaggcg cacgggcagc
     5041 aacgtgccga tgcaggcgcc gcgcgacaag tggtgggatg acgtcgtcaa gggccagccg
     5101 gagacatgcg aaccgacctg ggtgaacgcg gagcatccgc tgttcatcct gtacacgtcc
     5161 ggctcgaccg gcaagccgaa gggcgtgcag cactcgacgg gcggttacct gctgtgggcc
     5221 gtgctcacga tgaagtggac gttcgactac aagccgagcg atgtgttctg gtgcacggcc
     5281 gacgtcggct gggtgaccgg ccacacctac attacctacg gcccgctcgc ggtcggcgct
     5341 accgaagtga tgttcgaagg cattccgacc tatcctgacg ccggacgctt ctggaagatg
     5401 atccaggacc acaaggtcag cgtcttttac acggcgccga ccgcgatccg ttcgctgatc
     5461 aaggctaacg agggcaatcc ggcaactcac cccacaaagt acaacttgtc gggcctgcgc
     5521 atcctcggca cggtcggcga gccgatcaat cccgaagcct ggatgtggta ccacaccaac
     5581 gtggggggtg gtcgttgccc catcgtggac acctggtggc agacggagac cggcggtcat
     5641 ctgattacgc cactgcccgg cgtcacaccc accgtgcccg gctcctgcac gctgccgctg
     5701 cccggaatca tggcggcgat cgtcgacgag acaggacatg acgtgccgaa cgggcagggc
     5761 ggcctgctgg tgatcaagcg gccgtggccc gcgatgatcc ggacgatctg gggtgatccg
     5821 gagcggtaca agaagtcgta cttcccggag gaactcggcg gcacgctgta cctggccggc
     5881 gacggttcca tccgcgacaa ggaaaccact tacttcaaga tcgtcgggcg catcgacgac
     5941 gtgctgaacg tctccggtca ccgcatgggc acgatggaaa tcgaatccgc gctggtctcg
     6001 aacccgatgg ttgccgaagc cgccgttgtc ggtcgcccgg acgacatgac aggcgaggcg
     6061 atctccgcct tcgttgtcct caagcgagcg cggccaaccg gtgaggaagc gcagcagatc
     6121 gcgaaggaac tacgtgactg ggtcggcaag gaaatcggtc cgatcgccaa gcccaaggac
     6181 atccgcttcg gcgacaacct gccgaagacg cgatccggaa agatcatgcg gcgcctgttg
     6241 cgctcgatcg ccaagggcga agagatcaca caggacatct cgacgctgga aaacccagcg
     6301 atcctggatc aattaaaggt gacgctgtag cagctgggtt tcggtgaggc ttatgagagc
     6361 ggagcgaacg taaagcgagc ggatgcgagc ggccgtagcc aaaacccagc gatcctggat
     6421 cagttgaaag tgacgctgta agaaagtgac gctgtaagtc cgaagcgcga aagtttcgga
     6481 ggcagacggg ggcgatgagc cccgaacaaa gaggcgcaag gccccgtgag tcggatgagt
     6541 ccggcccacg gggtgaacgc ccgacggacg tgcggcagag tcgtgaagcg tgctgcatgg
     6601 caggtggaaa cggtgcgggc gagtctgcac cgaacgggga aaaggatggc gatggcattt
     6661 gcattgctgg cgctgcaaac gctggtgctg gtcgcgggcc tggccctgct cggccagggc
     6721 ctcgtcggcg ccttcaactg gtcgcgccgt cgtcagaacc tcgtctggcg tctgttcgag
     6781 gtcatcgcca gcccggtggt caaggttgtc cgactggtca cgcccaagtt tgtgctggac
     6841 cagcacattc cgatcgccgc attcatgctg ctgttcttcg cctacttctg gctcggcttc
     6901 gaacatcgtt cgacatgccg cgccgacacg gcgcaggcgg gctgcgaaaa gtgggccgcg
     6961 gcgtggagcg gcgcaacggt gaagcaatga cctggctcgc gcagaaactc aagctgtggt
     7021 ggcttcagcg ccaagccaac gctgcaactc ttttcaagcg cgttcccgat gcggtgcgtg
     7081 cctaccgcga catgctcgcg atcgaccccg gcaatcagat ggccggtctc atgctcggca
     7141 acctgttggc ggaagcgggt gaccgcgagg gtgccgtggc tcagctggag cagctgacgg
     7201 tcgtcaatcc gtcgtttgcc gacgcctggt tcaacctggg ctacctgcac gaccaggcgg
     7261 atcggctgac tgatgccgag cgatgcttcc ggcgggcggt cgagttgcgg ccttcgcttg
     7321 accgcgcatg gtacgggctg ggcctggtac tgatccgtga cggccgcctg accgaggcgg
     7381 tcgatgcgct aaagcagacc atcaagctgc agccgttcag tccctatggg tactaccagc
     7441 tcggcctgac ctatcaccat ctgggccgca atgaggacgc ctggcgtgtc cacaagcagc
     7501 tgaccgaatt cgaaccgaag ttttctgcga cgctgaagcg cgatctcgag cggacggtgc
     7561 cgcaggctgt tcccgcaatc aatccgaact cccaacccaa ggaggaaacg accgtcgacg
     7621 ccacttagtc caaggagaca gaagcctact taggagagaa catgcaactc accgaaaagc
     7681 ataaagagta ctggagcagg aatctgcgga tcaccgcgat cctgctcttc atctggttcg
     7741 tcgtcactta tgtgatggcg tacttcgcca ttccgctggc caagatcaac ttcttcggct
     7801 ggccgctgtc gttctacatg gcagcgcagg ggtccctgat catctatgtc ttactcattt
     7861 ggtactacgc gaaatacatg cgcaaactcg atcttgagta cggcgtggac gaaggagacc
     7921 tgaaatgagc gcgggcgtca gttcgaatcg tgccttcttc aaccagttga agaagtacta
     7981 ca
//
LOCUS       DATIRT010000215         4520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1505818, whole genome shotgun sequence.
ACCESSION   DATIRT010000215 DATIRT010000000
VERSION     DATIRT010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505818"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..1447
                     /locus_tag="VLN25_05030"
     CDS             92..1447
                     /locus_tag="VLN25_05030"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HSQ09963.1"
                     /translation="MAEPTLLQEVVEIVQGGLARPQSLWQLGVIAAGLIVGALLSRRV
                     QRRTRARVAAESQGFNLRTDVLRFSLEGIRRLAFPVSALLVMVAGGLLLRAGGLVQRV
                     SDVQLLRLALTLLAAMAAVRLTVYILRRSLPGSKWLGTFERWIALLIWIAVALHLTGV
                     FGDVSAALESVRLPVGRSNISLWDLLVGAASVVVTVLAALWLGSTVESRLMRTETLTA
                     NSRVVLARVLKALLLFFAVLVALSLVGIDLTVLSVFGGALGVGLGLGLQRIASNYVSG
                     FIILLDRSLSIGDMITVDKFYGRVAQINTRYTVIKALDGTETILPNEMLVSTPVVNHS
                     LSNREVRIVVKVAVAYSTDLDRALGILSECARSQPSVLRQPEPGAFVTALGADGVELE
                     IGFWIRDPEVTLAVRSEISREVLRRFASAGIQSPFSQRDVRILPAEAGAAGGSPVVKP
                     P"
     gene            1570..2769
                     /locus_tag="VLN25_05035"
     CDS             1570..2769
                     /locus_tag="VLN25_05035"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HSQ09964.1"
                     /translation="MYLLEFLDGGLLQLSWWQLILAGLLLTHITIAAVTIYLHRSQAH
                     RALDLHPAVANFFRLWLWLTTGMQTREWVSIHRKHHAKCETTEDPHSPQTRGLRTVLW
                     TGAELYMAEAENKETLSRYGHGTPDDWVERNVYSRYTWQGVGLMLGVNLILFGVAGLA
                     IWGVQMMWIPFFAAGVINGIGHYWGYRNYDAPDASTNISPWGILIGGEELHNNHHTHP
                     TSAKFSLKWFEFDLGWTYIRAMQVVGLAKVLRVAPVPKVAEPKPAIDLETLQAVITHR
                     YDLLAKYARNLTQAYRAELVTLELPDAERARFARLKGWLRKGDVSTLPAEEQRSLREL
                     MARSGVMKTLVEMRTELASTWEKSSASREQMLVHLQDWIARAEASRIRALEDAALRIR
                     SYRAVAA"
     gene            complement(2864..3031)
                     /gene="rpmG"
                     /locus_tag="VLN25_05040"
     CDS             complement(2864..3031)
                     /gene="rpmG"
                     /locus_tag="VLN25_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HSQ09965.1"
                     /translation="MAKGAREKIKLESSAGTGHFYTTSKNKKTTPDKLQLMKFDPVAR
                     KHVAYKETKLK"
     gene            complement(3085..3324)
                     /gene="rpmB"
                     /locus_tag="VLN25_05045"
     CDS             complement(3085..3324)
                     /gene="rpmB"
                     /locus_tag="VLN25_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HSQ09966.1"
                     /translation="MARVCQVTGKKPMVGNNVSHANNRTKRRFLPNLQYRRFWVESEN
                     RWVRLRLTNAALRTIDKKGLDVVLADMRARGEAVG"
     gene            complement(3581..4255)
                     /gene="radC"
                     /locus_tag="VLN25_05050"
     CDS             complement(3581..4255)
                     /gene="radC"
                     /locus_tag="VLN25_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HSQ09967.1"
                     /translation="MAIKDWPEGERPRERLLELGAQALSDAELVAVFLRTGTAGRSAL
                     DLGRDALARFGGLSGLLAARPDQLAELAGLGPAKSAQLQAVLELARRSLREEVQRDTV
                     LSSPGKVRDYLRMTLSGREHEVFVALFLDAQNRLLVADELFRGTLTQTSVYPREVVKR
                     ALAVNAGAVIFAHNHPSGIAEPSRSDELLTAALKQALGLVDVRVLDHLIVAGNGMTSL
                     AERGLI"
     gene            4285..>4520
                     /locus_tag="VLN25_05055"
     CDS             4285..>4520
                     /locus_tag="VLN25_05055"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HSQ09968.1"
                     /translation="MPESPRITSGSHVTLHYRIAVLVDGSEREVMSTFGAQPATLTVG
                     AGDLAEPLEARLLGLEEGARAQFELPAGEGFGAR"
BASE COUNT          726 a         1389 c         1504 g          901 t
ORIGIN      
        1 cctgcagcgc tttgcgccgt atttcgtcat gctcccgcat atgcatgcca ccgacgggtt
       61 cttcgctgcc gtgcttgagc gcagctgaag catggccgag ccgacgctgt tgcaggaagt
      121 cgtggagatc gtgcagggcg gcctcgcgcg gccgcagtcg ttgtggcagt tgggagtcat
      181 cgctgcgggg ctgatcgtcg gggccttgct gtcgcgccga gtgcagcgac ggacacgtgc
      241 ccgcgtcgcg gcggaatcgc agggattcaa cttgcgcacg gatgttctgc gattctcgct
      301 cgagggaatc cgccggcttg ccttcccggt cagtgctctt ttggtgatgg tggccggcgg
      361 ccttctgctg cgcgccggcg gactggtgca gcgggtgagc gatgtgcagt tgctgcggct
      421 ggcgctgacg ctgctggcgg ccatggcagc ggtgcgactg acggtctaca tcctgcggcg
      481 ttccttgccg ggctcgaagt ggctgggaac tttcgagcgc tggatcgcat tgctgatctg
      541 gatcgcggtg gcattgcacc ttaccggcgt gttcggtgat gtcagcgccg cactcgaatc
      601 ggtgcggctt ccggtgggcc ggtcgaacat cagcctgtgg gacctgctgg tcggtgccgc
      661 ttcagtcgta gtgaccgtgc tggcggcctt gtggctcggg tccaccgtcg aatcgcggtt
      721 gatgcgtaca gaaaccctga cagccaactc gcgcgtcgtg cttgcccgag tgctgaaggc
      781 gctgctgctc ttctttgccg tgctggttgc actgtcgttg gtcggcatcg atttgaccgt
      841 gctgtcggtg ttcggtggtg cgctgggcgt cggcctgggg ctgggcctgc agcgcattgc
      901 cagcaactat gtcagcggtt tcatcatcct gctcgatcgc agcctctcga tcggcgacat
      961 gatcaccgtc gacaagttct acggccgggt tgcacagatc aacacacgct acaccgtcat
     1021 caaggcgctc gatggaacgg aaacgatcct gccgaacgaa atgctggtct cgacgcccgt
     1081 cgtcaaccac tcgctcagca atcgcgaagt gcggatcgtt gtaaaagtgg cggtggccta
     1141 ctcgacggac ctcgatcgcg cgctcgggat cctgagcgag tgcgcgcgtt cgcagccaag
     1201 tgtgctcagg cagccggaac cgggtgcgtt tgtcaccgca ctgggtgctg acggagtcga
     1261 actcgagatc gggttctgga ttcgagatcc cgaggtcacg ttggcggtgc ggtccgagat
     1321 ttcgcgcgaa gtgctgcgtc gttttgcctc agccggcatc cagagtccgt tctcgcagcg
     1381 cgacgtgcga atccttcctg cggaagcggg ggctgcgggc ggatcgccgg tcgtcaaacc
     1441 gccttgatta cgggcaatcc gggcggatct tctgctttga gggcggcgag ggccggcagg
     1501 tacaatccgg ctttgaaaaa ttggtcacgc cgcggaatgc acgcgacggc gcattgagga
     1561 cataaatcca tgtatttgct tgaatttctg gacggcgggc tgcttcagtt gtcgtggtgg
     1621 caactgatcc tggcggggct gctgctgacc cacatcacga tcgcggcggt gacgatctac
     1681 ctgcatcgta gccaggcaca tcgggcgctc gacctgcacc ctgcggtggc gaatttcttt
     1741 cgcctgtggc tgtggctgac caccggcatg cagacgcgcg agtgggtttc gatccaccgc
     1801 aagcaccacg ccaagtgcga aaccaccgaa gacccgcaca gtccgcagac gcgcggcctc
     1861 aggacggtgc tgtggaccgg cgccgagctg tacatggccg aggcggagaa caaggagacc
     1921 ttgtcacgct acggccacgg cacaccggac gactgggtcg aacgaaatgt ctactcccgc
     1981 tacacgtggc agggagtcgg cctgatgctg ggcgtgaatc tcatcctgtt cggcgtggcc
     2041 ggcctggcca tctggggcgt gcagatgatg tggatcccgt tcttcgcggc gggcgtcatc
     2101 aacggcatcg gtcactactg gggttatcgc aactacgacg cgccggatgc cagcaccaac
     2161 atctcaccgt ggggcatact gatcggcggc gaggaactcc acaacaacca ccacacgcat
     2221 ccgacgtcgg cgaagttctc gctcaagtgg ttcgagttcg acctgggctg gacgtatatc
     2281 cgcgcgatgc aggtggtggg tcttgccaag gtcctgcgcg tcgcaccggt gcccaaggtc
     2341 gccgagccca agccggcgat cgacctcgag acgctgcagg ctgtgattac ccaccgatac
     2401 gacctgttgg ccaagtacgc gcgcaacctc acgcaggcct accgtgccga gctggtcacg
     2461 ctcgaactgc ccgacgccga gcgcgcccgc tttgcccgcc tcaagggctg gctgcgcaag
     2521 ggcgacgtgt cgacgttgcc ggccgaggag cagcgctcgc tcagagagct gatggcgcgc
     2581 agcggtgtca tgaagaccct ggtggagatg cgcaccgaac tggcgtcgac ctgggagaag
     2641 agctcggcat cgcgtgagca gatgctggtg cacctgcagg actggatcgc acgcgccgag
     2701 gcgagccgca tccgtgcact cgaagacgcg gcgttgcgta tccgcagtta tcgggctgtc
     2761 gcggcctgat ccttgggaca gcggcagtcg gacggctgca cgcgaaaaaa agcccggctc
     2821 atggccgggc ttttttgttt ccccggtccc cggggcgatc aggctacttg agcttcgttt
     2881 ccttgtaggc cacgtgcttg cgcgctaccg ggtcgaactt catcagttgc agcttgtcgg
     2941 gagtggtctt cttgttcttc gaagtcgtat aaaaatggcc ggtgccggcc gaggactcga
     3001 gcttgatctt ttcgcgggcg cctttagcca tgatgctctc ctgaaagggt gctggacggc
     3061 ttgggagtgt cgttccggcg ggactcagcc gacagcttcg ccgcgggcac gcatgtccgc
     3121 gagcacgaca tccaggccct tcttgtcgat cgtgcgcaag gccgcgttgg tcaggcgcag
     3181 gcgcacccag cggttctcgc tttccaccca gaagcggcgg tactgcaggt tcggaaggaa
     3241 ccggcgcttg gttctgttgt tggcgtggga aacgttgttt ccgaccatcg gcttcttgcc
     3301 ggtgacttga cacacacgtg ccatggcttg ctcctgattc tgttctggcg gacggctgaa
     3361 ctcttgcgga agtcgtgcat gagctccggg catttagccc agccacctgg ccgcggtctt
     3421 ggtcccgcag gttctggctg caggtaagag atggatccaa ggtgacgcaa agccgcacat
     3481 tatatgcgtt ttatagtcgt gccgccaagc gtcgggccgg tctgaccgtg gcgcggactg
     3541 tcgttcgact tccccgtttc cggcgctctg tgggttcccg tcaaatcaat cctcgctcgg
     3601 ccagtgacgt catgccgttg ccggccacta tcagatggtc gagcacacgg acgtcgacca
     3661 gcccgagtgc ctgcttgagg gctgctgtca gcagttcgtc gctccggctg ggttcggcta
     3721 tgcccgacgg gtggttatgc gcaaagatga cggcaccggc gttgactgcg agggcgcgct
     3781 tgaccacctc gcgcgggtag acgctggtct gcgtcaaggt cccgcgaaac agctcgtcag
     3841 cgacaagcaa tcggttctgg gcatcgagaa agagggcgac gaacacttcg tgctcacggc
     3901 cactgagcgt catgcgcagg tagtcacgca ccttgcccgg ggacgacaac acggtgtcgc
     3961 gctggacctc ctcgcgcaac gagcgccggg cgagttccag cacggcctgc aactgcgctg
     4021 acttggcggg tccgaggccg gccagctcgg ccagttgatc gggccgggcg gccagcaggc
     4081 cgctcagtcc gccgaaacgc gccagagcgt cgcgtcccag gtccagcgcc gagcgcccgg
     4141 ctgtcccggt ccgcaggaac acggcgacca gttcggcatc cgacagcgct tgcgcgccca
     4201 gttcgagcag gcgctcgcgc ggccgttccc cctcgggcca gtccttaatc gccatgcgtg
     4261 ttgcctcgac tagaattcac gaccatgcct gaatccccgc ggattacgtc cggctcgcat
     4321 gtcacgctgc attatcgtat cgccgtgctg gtcgacggat cggagcgcga agtcatgagc
     4381 acgtttggtg cgcagccggc gaccttgaca gtcggtgcgg gtgatctggc cgagccgctc
     4441 gaagcccgtc tgcttggcct cgaggaaggc gcccgcgcac agtttgaact gcccgctggc
     4501 gagggcttcg gagcccgcag
//
LOCUS       DATIRT010000216         3321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_424530, whole genome shotgun sequence.
ACCESSION   DATIRT010000216 DATIRT010000000
VERSION     DATIRT010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424530"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VLN25_05060"
     CDS             complement(<1..698)
                     /locus_tag="VLN25_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09969.1"
                     /translation="MLEVVVSAVASVGAVGLLLAGGLFARSLLPGATPLVSQFALAED
                     PATAIQPLAQRYLRWLTLLWAATLLVGAYIVARRAAGDAVFPPGIGYFGPLALAALLF
                     FGERSVRRVMFGTQSVGPVARQWRIATRVMREELGWLGGGMPGRAEAVVLLPDPSRTL
                     IVWSDARCSVGALQAVALRLSRSLPPGRRVLVECEDRAVFLWAVLAVWAARRTVVMPP
                     PDLKATQGHSPRSH"
     gene            complement(705..965)
                     /locus_tag="VLN25_05065"
     CDS             complement(705..965)
                     /locus_tag="VLN25_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016724058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HSQ09970.1"
                     /translation="MDQLEREIAESIVDALALPDVRIEDLSTDTPLYGEGMGLDSIDI
                     LEVALIVSKRYGFELRSDDPLNEQIFRSLGSLTEHVRRHRSK"
     gene            complement(1025..1288)
                     /locus_tag="VLN25_05070"
     CDS             complement(1025..1288)
                     /locus_tag="VLN25_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09971.1"
                     /translation="MAAVESAILRAGNGLGWQMKVEKPGLIVGTLSLRTHTAVVEIPF
                     STTTYSILYRSSVNLEQQGDMIHKNYNGWVQNLDQAIRRQLTN"
     gene            1662..2771
                     /locus_tag="VLN25_05075"
     CDS             1662..2771
                     /locus_tag="VLN25_05075"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSQ09972.1"
                     /translation="MSTFKRTLTATEARFEAQRIAFAPVIFQVVQVMRRTGLLRALVS
                     ARDWSDQAELERVTGLSSYATTVLLESALSAGVVERDEGRDFQAAGGIRWRTTAVGRF
                     IERDPLVRINMDFVDDVCYRSLAHLEAALVEGRPAGLKELGPWKTFYEGMADMPEPAR
                     TSWFTYDHYYSDSAFPAALDVLAMRDLRHVADVGANTGNFAIAYLHRVPDGTVTLCDL
                     PQQLQLARSNLERHGLLERARFHPCDLLNGTSRLPEGEAAPDAFWMSQFVCCFSEAQI
                     VDILQRAATAVRAGGSIFVLDTFWDDQRHAIAAYCLVNTSPYFATVANGDSRMYRLDV
                     FLDLASQAGLRAEKTWSDLGWCHTLIELRPLRSFD"
     gene            complement(2874..>3321)
                     /locus_tag="VLN25_05080"
     CDS             complement(2874..>3321)
                     /locus_tag="VLN25_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442637.1"
                     /note="activates fatty acids by binding to coenzyme A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HSQ09973.1"
                     /translation="TAKAFTPDGWLRTGDMGVMDEHGGIRLTDRKKDMIVVSGFKVFP
                     NEVEDVLTTHPGVAEAAAVGIPDERSGEAVKVVVVRNDPALTEAEVLAHCKKHLTGYK
                     IPRIVEFRSEPLPKTNVGKILRRELRDSSAATLAAARATPAASTGA"
BASE COUNT          630 a         1071 c          990 g          630 t
ORIGIN      
        1 aaatgggatc tcggcgaatg gccctgtgtg gccttgaggt cgggcggcgg catcaccacg
       61 gtgcgtcggg ctgcccaaac ggctagcacg gcccagagga acaccgcgcg gtcttcgcac
      121 tccaccagca cgcgtctgcc gggcggcagt gacctcgaaa gtcggagggc gaccgcctgt
      181 agggcaccca cggaacaccg ggcgtcgctc cagacgatca gcgtgcggct cgggtccggc
      241 agcaggacga ccgcctctgc gcgaccaggc atgcctcctc ccaaccaacc aagctcttct
      301 cgcataacgc gtgtagcgat tcgccactgc cgggccacgg ggccgaccga ttgcgttcca
      361 aacatcacgc gacgcacgct gcgctcgcca aagaagagca gcgcggcgag cgcgagcgga
      421 ccgaagtagc caattccggg cgggaacact gcatcgccgg cggcgcgtcg tgcaacgatg
      481 tatgcgccga cgagcaaggt ggctgcccac aagagcgtca gccatcgcag gtaacgctgc
      541 gcgagtggct ggatggcggt cgccggatcc tcagccaatg caaactgcga gactagtggc
      601 gttgctcccg gcaacaacga gcgcgcgaac agaccacctg cgagcaacag tcccacggcc
      661 ccgacggaag ccaccgcgga aacgacgact tcgagcatca ccgactactt gctacggtgg
      721 cgacgcacat gctcggtgag cgatccgagc gaccggaaga tttgttcatt gagagggtcg
      781 tcagaacgca gttcgaagcc gtagcgcttg gatacgataa gcgccacttc gagtatgtcg
      841 atcgaatcga gccccatgcc ctcaccgtac aacggcgtgt ccgtgctcaa gtcttcgatg
      901 cgcacgtcag gcagcgcgag cgcatcgacg atcgattcag cgatttcacg ttccagttga
      961 tccatctgcc gccctgcaca cgtcaccccg acggggcggt cgccgcacca ttcccgcccg
     1021 tcggtcaatt cgtcaactgg cgtcggattg cctgatccag gttctggacc catccgttgt
     1081 agttcttgtg gatcatgtca ccctgctgtt caaggttgac gctgctccgg taaaggatgc
     1141 tgtacgtcgt cgtcgagaat ggaatctcga ccacggccgt gtgcgtacgc aggctcagtg
     1201 tgcccacaat cagtcccggc ttctcgacct tcatttgcca cccgaggccg ttgccggcgc
     1261 ggaggatcgc gctctcaact gcagccattg aatccttttg tccggccggc agactgatcg
     1321 gcgcatcctt gacgttgagg atgggaactg tgcggcaccc gaccaccagt agtgacgcgg
     1381 cagcgacggc gacagcggca agcattcgaa aacgcataat cacctctccc ttcgcacacg
     1441 aaacaagacg atttctaatc ggtgcgcatt tgttgcaccg cacagtctcg cagacctcta
     1501 tttcattgtc ttgcccggac tgaaaataat attgatggtc agcaagaggc aagtggtaaa
     1561 tgcggggaat cttacatcga cggtgtcatt gttgaagtgt gttgtattcg gcaatactgc
     1621 cgtcgtcgtc gcgtttacgc tgcgatccca tctggagtga tgtgtccaca ttcaagcgca
     1681 cgttgacggc gaccgaggcg cgcttcgagg cgcaacgcat tgcgttcgcc ccggtcattt
     1741 tccaggtggt acaggtcatg cgacgaaccg ggctgctgcg cgcactggtc agtgcgcgcg
     1801 actggtcgga ccaggccgaa ctcgaacgcg ttaccgggct gtcgagttac gcgaccactg
     1861 tcctgctgga gtcggcactg tccgccggag tagtagagcg cgacgaaggc cgcgactttc
     1921 aagctgcagg aggaattcgc tggcgcacca ccgctgtcgg ccgcttcatc gagcgcgacc
     1981 cgctggttcg catcaacatg gacttcgttg atgacgtctg ctatcgcagc ctcgctcacc
     2041 tcgaggcagc attggtcgag ggccgccccg ccggattgaa ggaactcggt ccctggaaaa
     2101 cattctacga aggcatggca gacatgcccg agcctgcccg gaccagctgg ttcacctacg
     2161 atcactacta ctccgacagc gcgttccccg ctgcgcttga cgtgttggcc atgagagacc
     2221 tgcgacacgt ggccgacgtt ggagccaata cgggcaactt tgcaattgcg tacctgcatc
     2281 gcgtccctga tggcacggtc acgctgtgtg atctcccgca acagttgcag ctggcacgct
     2341 cgaacctcga acgtcatgga ctgctggaac gggccagatt ccatccctgc gacctgctga
     2401 acggaacgag ccgactgccg gagggagaag cggctccgga tgccttctgg atgagccagt
     2461 tcgtctgctg cttttccgaa gcccagattg tcgacatact ccagcgcgcg gccaccgccg
     2521 ttcgtgccgg cggatcgata ttcgtactgg acacgttctg ggacgaccag cgccacgcca
     2581 ttgccgcgta ttgcctggtc aacacctcgc cttacttcgc gacagtggcg aacggcgaca
     2641 gccggatgta ccggctcgac gtctttctcg atcttgccag tcaggccggg ctacgcgcgg
     2701 aaaagacctg gagcgatctc ggctggtgcc acacgctgat cgaactccgt ccattgcgaa
     2761 gtttcgactg aggcaccgaa cccacgttca atgctgtcat tcaccggatt cgtcggcgcc
     2821 tagaccgcga aggtcactaa ttgcagcgca gcgacatcgt cgccatgcac aaactaggct
     2881 ccggtggatg ccgcgggcgt tgctcgtgcc gcagcgagcg tcgccgcgct gctgtcgcgc
     2941 agttcgcgtc gcagaatctt gccgacattg gtcttcggca gcggctcgct gcggaactcc
     3001 acgatgcgcg gaatcttgta gcccgtcaga tgcttcttgc agtgcgcgag cacctcggct
     3061 tccgtcagcg ccgggtcatt gcgcaccacc accaccttga ccgcttcgcc ggagcgctca
     3121 tcgggaatgc ccacggccgc ggcctccgcc acgccggggt gcgtcgtcag cacatcctcg
     3181 acctcgttgg gaaacacctt gaatccggac accacgatca tgtccttctt ccggtccgtg
     3241 aggcggatcc cgccgtgttc gtccatcact cccatgtcgc cggtgcgaag ccagccgtcg
     3301 ggggtgaacg ccttggcggt c
//
LOCUS       DATIRT010000217         2980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_719549, whole genome shotgun sequence.
ACCESSION   DATIRT010000217 DATIRT010000000
VERSION     DATIRT010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719549"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1524
                     /locus_tag="VLN25_05085"
     CDS             136..1524
                     /locus_tag="VLN25_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSQ09974.1"
                     /translation="MGVAIALVVLVIGSLLFHFWSPWYFTPIASNWSAIDDTISITFW
                     VTGAVFVLVNLFMAYAIYRYRHRANARAQYEPENKKLEWWLLAVTTVGVAAMLAPGLF
                     VWAKFVDVPKDALVVEVVGQQWNWAYRLPGKDGKLGAVATRHISDANPLGVVPTDPDG
                     QDDLMVLSPELHLPLGKPIKVLLRSNDVLHNFSVAQIRVKMDFVPGMVTQFWFTPTRT
                     GNFDLLCEELCGIGHFAMRGRMVVEEEGPYQAWLAQQPTFAQLAARRGGGDAAAGQAL
                     FATCAACHGAQGEGNRELNAPKLAGQGSWYLRRQLANYKQGARGTNDKDQYGKVMAPM
                     AAVLADDAAIDNVVAYIAKLPDTPAPVTVKGNPANGKQRFVTCGACHGPDGLGIQATN
                     APRLRGMSDWYAVTQLKNFRDGVRGAHPQDLHGAQMALFAAGLTTDQAILDIVAYIDT
                     LADRPAKGGKGR"
     gene            1589..>2980
                     /locus_tag="VLN25_05090"
     CDS             1589..>2980
                     /locus_tag="VLN25_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HSQ09975.1"
                     /translation="MSAHAAHEESDFAPPAEVPEVELYHPKTWLGQYVFSQDAKIIAI
                     QYACTAIGIGLVALVLSWLMRLQLAWPNQFSFINPSNYLQFVSMHGMIMVVYLLTALF
                     LGGFGNYLIPLMIVARDMVFPYVNMLSYWIYLFAVLLLVASFFVPGGPTGAGWTLYPP
                     QAILDGTPGATWGIILMLVSLAFFIIGFTMGGLNYVVTVLQARTRGMTLMRLPLTIWG
                     IFMATILALLAFPALFVSAIMMTLDLTLGTSFFMPAMVSKGQQLDHSGGSPILFQHLF
                     WFFGHPEVYIVALPAFGIVSDLISVHARKNIFGYRMMVWAILIIGGLSFIVWAHHMYV
                     SGMNPYLGFFFATTTLIIAIPTAIKVYNWVLTLWRGNIHLTVPMLFAIGFIFTFINGG
                     LTGLFLGNVTIDLPLSDTMFVVAHFHMVMAIAPIMVIFGAIYHWYPKITGRMLNDTMG
                     HIHFWITFVGSYAI"
BASE COUNT          523 a          929 c          922 g          606 t
ORIGIN      
        1 tcactgagac ttaccaagaa atcctacagg tagcgggcgc ggacggccgg ggcatacgct
       61 gcgacgatgc cccgagcgcg gctccggcga agggtgcgtg cacccgtctg gcgtccaatc
      121 aacgaggact cgctaatggg cgtggcaatc gcgttggttg tgctggtcat tggctcgctg
      181 ctgttccatt tctggagtcc ctggtacttc acgccgatcg cctccaactg gagcgcgatc
      241 gacgacacga tctcgattac gttctgggtc accggcgctg tcttcgtgct ggtcaacctg
      301 ttcatggcct acgcgattta ccgctatcgg caccgtgcca atgcgcgggc gcagtacgaa
      361 cccgagaaca agaagctcga gtggtggctg ctggcagtga ccacggtggg cgtggccgcc
      421 atgcttgcgc cggggctctt cgtctgggcg aagttcgtcg atgtgccgaa ggatgcactc
      481 gtcgtcgagg ttgtgggcca gcaatggaac tgggcctacc gcctgccagg aaaggacggg
      541 aagctcggcg ccgtcgccac gcggcacatc agcgacgcga atccgctcgg cgtcgtcccg
      601 acggatcccg acggccagga cgacctcatg gtgctgagcc ctgaattgca tttgccgctg
      661 ggcaagccga tcaaggtgct attgcgctcg aatgacgtct tgcacaattt ctcggtggcg
      721 cagatccgcg tcaagatgga cttcgttccc ggcatggtca ctcaattctg gttcacgcca
      781 acgcggaccg gcaacttcga tctgctgtgc gaggaactgt gcggcatcgg ccacttcgcc
      841 atgcgcggac ggatggtggt cgaggaagaa ggaccgtacc aggcgtggct ggcgcaacag
      901 ccgaccttcg cgcagctcgc ggctcggcgc ggcggcggtg atgccgccgc cggccaggcg
      961 ctgttcgcga cctgcgctgc gtgccacggt gcgcagggtg agggcaatcg cgaactcaat
     1021 gccccgaagc tcgcgggtca gggcagctgg tatctccgac gccagctggc gaactacaag
     1081 caaggcgcgc gtgggacgaa cgacaaggat caatacggca aggtcatggc gccgatggct
     1141 gccgtgctcg ccgacgacgc cgcgatcgac aacgtcgtcg cctatatcgc gaagctgccg
     1201 gacactccgg cgcccgtcac cgtcaaaggc aatccggcca acggcaagca gcgattcgtc
     1261 acctgcggcg cctgccatgg tccggacgga ctcggtatcc aggcgaccaa tgcgccgcgg
     1321 ctcaggggca tgagtgactg gtacgccgtc acgcagctca agaacttccg agatggcgtg
     1381 cgtggcgcgc atccgcagga tcttcacggc gcgcagatgg cgctgttcgc tgcgggactg
     1441 accaccgacc aggccatcct cgatatcgtt gcttatatcg acacactcgc agatcggccg
     1501 gccaagggcg ggaagggccg ttgaccaaag tctccacaca ccactgaaac ggagcgcgac
     1561 agctccggaa acgcgaacag gaaagctgat gtcagcccac gctgcccacg aggaatcgga
     1621 cttcgcaccg cctgcggaag tgccggaggt cgagctctac catccgaaga catggcttgg
     1681 ccaatatgtc ttcagtcagg acgccaagat cattgcaatc cagtacgcgt gcaccgcgat
     1741 cggcatcggg ctggtggctc tcgttctgtc ctggctgatg cgcctgcagc tggcgtggcc
     1801 gaatcagttt tcgttcatca atccgagcaa ctacctgcag ttcgtcagca tgcacgggat
     1861 gatcatggtg gtctacctgc tgacggcgct gttcctcggt ggcttcggca actacctgat
     1921 cccgctgatg atcgtggcgc gcgacatggt gttcccgtac gtgaacatgc tgtcctactg
     1981 gatctacctg ttcgccgtgc tgttgctggt ggcgagcttc ttcgttcccg gcggacccac
     2041 cggcgccggc tggacgctgt atccgcctca ggcgatcctc gacggcacgc cgggtgcgac
     2101 ctggggcatc atcctgatgc tggtctcgct cgcatttttc atcatcggct tcacgatggg
     2161 cggcctgaac tatgtcgtga ccgtgctgca ggcgcgcact cgcggcatga cgctgatgcg
     2221 gctgccgctg acgatctggg gcattttcat ggcgacgatc ctggcgctgc tggcgttccc
     2281 ggcgctgttc gtcagcgcca tcatgatgac gctcgacctg acactaggca ccagcttctt
     2341 catgccagcg atggtgtcca agggccagca gctggaccat tcgggcggca gcccgatcct
     2401 gttccagcac ctgttctggt tcttcgggca tcccgaggtc tacatcgtcg cgttgcctgc
     2461 gttcggcatc gtctctgacc tgatcagtgt gcatgcgcgc aagaacatct ttggttaccg
     2521 catgatggtg tgggcgatcc tgatcatcgg cggcctgtcg ttcatcgtgt gggcccacca
     2581 catgtacgtc agcggcatga atccgtacct gggcttcttc tttgcgacaa cgacgctgat
     2641 catcgcgatc ccgacggcca tcaaggtcta caactgggtg ctgactctct ggcgcggcaa
     2701 catccacctg acggtgccga tgctgttcgc gatcggtttc atcttcacgt tcatcaacgg
     2761 tgggctgacg gggctttttc tcggcaacgt cacgatcgac ctgccgctct cggacacgat
     2821 gttcgtcgtt gctcacttcc acatggtgat ggcgatcgcg ccgatcatgg tcatattcgg
     2881 cgccatttat cactggtatc ccaagatcac cggccgcatg ctgaatgaca cgatggggca
     2941 catccacttc tggatcacgt ttgtcggcag ctacgcgatc
//
LOCUS       DATIRT010000218         7140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1407882, whole genome shotgun sequence.
ACCESSION   DATIRT010000218 DATIRT010000000
VERSION     DATIRT010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7140
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1407882"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VLN25_05095"
     CDS             <1..459
                     /locus_tag="VLN25_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HSQ09976.1"
                     /translation="WRAICPDLTIRSTFIVGFPGESEAEFDHLLDFLREAELDRVGCF
                     AYSPVAGAAANALVGPLPDEVREERRARFMQVQEEISARRLQRKVGTRQRVLLDEVGP
                     TVALGRSAADAPEIDGVVFVKAGRRKLAVGDFVDVTITSADAHDLHGVAI"
     gene            456..1100
                     /locus_tag="VLN25_05100"
     CDS             456..1100
                     /locus_tag="VLN25_05100"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HSQ09977.1"
                     /translation="MSTRYVLYTANRSGSASVEVMLGALGLDHDLKDATPWSDPPGPC
                     FEELKTVNPLGQLPTLITPGGEILTESVAVLWTLLERHPSAWVPAPSTRDRATCLRWM
                     QFASSTVYAAVGAADYPARWTTSRSAAARAAVERAAVARMKLGWDIIARKFPQKRGFM
                     LGERPKVCDVYLANLSRWWKMRDYLKDSHPSFAAMMQRVDRLPEVAPVWKRNWS"
     gene            1111..1716
                     /locus_tag="VLN25_05105"
     CDS             1111..1716
                     /locus_tag="VLN25_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09978.1"
                     /translation="MRDIRKFFAGVAFVLAAVAAPAAAQTTLSLGFGYAGSSGIDNAA
                     TEASAEVRSTAVWNLALGKLLDGTREGQLLFQQQSTTLDPGGGAAPFDLTIRHLHLGG
                     TVFFDDTVGKGAYVVGGLGATQFSPSTTGLGSEVRPSLSLGIGYSWPLAERIDLRAEV
                     RGFFVLVNSNGDFLCSGGCVVLLTSDLFSQVQATIGILGRF"
     gene            1843..3024
                     /gene="bktB"
                     /locus_tag="VLN25_05110"
     CDS             1843..3024
                     /gene="bktB"
                     /locus_tag="VLN25_05110"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketothiolase BktB"
                     /protein_id="HSQ09979.1"
                     /translation="MREVVLVSGARTAIGTFGGSLKEVPPTQLGATVVAEALKRAGVK
                     GDEVGHVVFGHVLNTEPRDMYLGRVAALDGGVSQEAPCLTLNRLCGSGLQAIISAAQT
                     IMLGDADIAIGGGAECMSRGPYIVPAGRWGQRMGDMKAIDMMVGALHDPFDVIHMGVT
                     AENVATKWGITRQEQDALAVESHRRAAAATKAGHFKSQIVPIEVKGKKGPVVFDTDEH
                     FREGTSMDDMAKLKPVFAKENGTVTAGNASGINDGAGAVVLMERRLAEKRGLKPLARL
                     VSYGHAGVDPKYMGIGPVPSTRKALERAGLKVADLDVIEANEAFAAQACAVAKDLGFP
                     ADKVNPNGSGISLGHPIGATGAIITIKALYELQRVGGRYALVTMCIGGGQGIAAIFER
                     I"
     gene            3168..3671
                     /locus_tag="VLN25_05115"
     CDS             3168..3671
                     /locus_tag="VLN25_05115"
                     /inference="COORDINATES: protein motif:HMM:NF017736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HSQ09980.1"
                     /translation="MDPRLAELLKEQRSLWVRRGKEELRATYWTAAQGRNQDEYLQLC
                     WIMRDVQADRVFAMDRSLLDTLAGIQAWLQRSGVNRPIELHSGYRTGRTNRATEGAAL
                     SSRHVVGQAADISVAGVSSVKLAGLSSVLGRGGTGFYVGRGFVHVDTGNERVWIDQKR
                     KPAAPTG"
     gene            complement(3706..4644)
                     /locus_tag="VLN25_05120"
     CDS             complement(3706..4644)
                     /locus_tag="VLN25_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HSQ09981.1"
                     /translation="MSREHLTRFAWLSIAAALATIAIKTGAWWLTGSVGLLSDALESI
                     VNLVAAGMTLWMLSLSARPPTEEHAYGYSKAEYFASGLEGALIFAAALAIAWAAIDRL
                     LHPQPLERVGLGIGLSLVATAINFAVARVLLVAARRYRSIALEADAHHLMTDVWTSAG
                     VVIAVGAVAVTHWNWLDPLIALAVAGNIVFTGFSLVRRSALGLLDRALPGEQRAAIEA
                     VLQAHRAQHIEFHALRTRAAAGRSFVSMHMLVPGAWTVQQAHDVAESIEQEIATAVPG
                     ASVSTHIEPREDPASYLDIDLDRTHGSAGITAPGPR"
     gene            complement(4685..5557)
                     /gene="serB"
                     /locus_tag="VLN25_05125"
     CDS             complement(4685..5557)
                     /gene="serB"
                     /locus_tag="VLN25_05125"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HSQ09982.1"
                     /translation="MKDLVIQGPSLSPEALDTFKVVCMPERITVTGRSARCVSVHDDL
                     ESRKAVRGLGDYWKLDVAFVDPAWRLSDFRLLALDMDSTLITIESLDEVAAYAGKGEE
                     VAAITEAAMRGEITDYRESLRRRVAMLAGVDAALLQRVYDEKLALNDGAEPLVATCRA
                     AGLRALLVTGGFTFFTERIQRRLGFDATRSNELGIVDGRLTGTVTGPAGGDILDAEGK
                     AQAVRDQCAELACATDRAIVIGDGANDLTMMALAGISVAWRAKPVVREKATFALSHAP
                     LDGVLAWFGDSASP"
     gene            complement(5588..>7140)
                     /locus_tag="VLN25_05130"
     CDS             complement(5588..>7140)
                     /locus_tag="VLN25_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012364090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HSQ09983.1"
                     /translation="CGDVGFGKTEVALRAAFAAVAGGKQVAVLCPTTLLAEQHFQTFS
                     DRFSQWPVNIVELSRFRSTKEVAAAIAGMNAGTVDIVIGTHKLLSGDVRFERLGLVII
                     DEEHRFVVRQKERLKSLRAEVDVLTLTATPIPRTLAMSLEGIRDFSVIATAPQRRLAI
                     KTFVRKESASLMREAVLRELKRGGQVYFLHNEVETIENRRARLAELLPEARIEIAHGQ
                     MPERDLERVMRDFYQQRFNVLLCSTIIETGIDVPTANTIVIHRADKFGLAQLHQLRGR
                     VGRSHHQAYAYLMVPDEGGLTKNAEKRLEAIQNLEELGSGFYLAMHDLEIRGAGEVLG
                     ENQSGNMQEVGFDLYTQMLNAAVRALRSGREPDLLSPLAAVTEINLHVPALLPPDYVN
                     DVHQRLSMYKKLASCDDEDGLIHLQEELVDRYGKLPEPARALIDTHRLRLVAERLGVK
                     KIDASGDALVLQFVPNPPFDTARLIALMQRSRTMRLTGPERLRIEEKTPNLEARLQRL
                     REVFKALA"
BASE COUNT         1194 a         2310 c         2444 g         1192 t
ORIGIN      
        1 tggcgcgcga tctgcccgga cctcacgatc cgctccactt tcatcgtcgg tttcccgggc
       61 gagagcgaag cggaattcga tcacctgctc gactttctgc gagaggctga actcgaccgg
      121 gtcggctgct ttgcctactc gccagtggca ggtgccgccg ccaatgcctt agtcggcccg
      181 ctgccggacg aggtgcggga agaacgccgt gcccgcttca tgcaggtgca ggaagagata
      241 tcggcccggc ggctgcagcg caaggtcgga acgaggcagc gtgtgcttct cgacgaggtc
      301 ggtccgaccg tcgcgttggg ccggtccgcg gcggatgcgc cggagatcga cggcgtggtc
      361 ttcgtcaagg cgggacgccg caagctggcg gttggcgact tcgtcgacgt gacgatcaca
      421 tcggccgatg cgcacgatct gcatggagtc gcgatatgag cacgcgctac gtcctgtaca
      481 ccgccaaccg ttcgggctcc gccagcgtcg aagtgatgct tggggcgctc ggactcgacc
      541 acgacctgaa ggacgccacg ccgtggagcg atccgcccgg tccgtgcttt gaggaactga
      601 agacggttaa cccactcggc cagctgccga ccctgatcac gcccggtggc gagatcctga
      661 ccgagagcgt ggcggtgttg tggaccctgc tcgaacggca tccgagcgcg tgggttccgg
      721 cgccgtcgac gcgcgaccgg gccacgtgcc tgcgctggat gcagttcgca tcgtcgaccg
      781 tctacgccgc ggtgggagcg gccgactatc cggcgcgctg gaccacatca cgttcggcgg
      841 cggctcgcgc ggcggtcgag cgggccgccg tggcccggat gaaactgggc tgggacatca
      901 tcgctcgcaa attcccgcag aagcgcggat tcatgctggg cgagcggccg aaggtctgcg
      961 atgtgtacct cgccaacctg tcgcgctggt ggaagatgcg cgactacctg aaggactcgc
     1021 atccttcatt cgcggcgatg atgcagcggg tggatcggct gccggaggtc gccccggtgt
     1081 ggaagcggaa ctggtcatga cgggcgcggc gtgagggaca tacgcaagtt cttcgcaggc
     1141 gtggcgttcg tgctggccgc ggtggcggcg ccggcggcag cgcagacgac gctgtcactc
     1201 ggcttcggct atgcaggcag cagcggcatt gacaacgccg cgaccgaagc gtcggccgaa
     1261 gtccgcagta cggctgtgtg gaaccttgcg ctcggcaagc tgctcgacgg aacccgcgag
     1321 ggccagctgc tttttcagca gcagtcgacg actcttgatc cgggcggcgg cgcggcgccc
     1381 ttcgacctga cgatccgcca cctccatctg ggtgggacgg tgttcttcga tgacacggtc
     1441 ggcaagggag cgtacgtcgt gggcgggctc ggcgccacgc agttcagtcc gagcacgacc
     1501 ggcttaggct ccgaggtcag gccgtcgctc agcctcggga tcggttactc ctggccgctc
     1561 gcggagcgca tcgacctgcg cgccgaggtg cgcggctttt tcgtcctcgt caacagcaac
     1621 ggcgacttcc tctgctccgg cggctgtgtc gtcttgctca ccagcgatct cttctcgcag
     1681 gttcaggcga ccatcggcat cctcggccgc ttctagcatc cacggcgccg gcactggggg
     1741 accctgcgcc tgaatccagg tcaagcagcc caggcggggg cgtgcaagaa ttcccgcttc
     1801 cggccctcgg gccgatgaca acccgctcca aagggagatc ccatgcgcga agtcgtctta
     1861 gtcagcggag cccgaaccgc tatcgggacg tttggtggca gcctcaagga ggtgccaccg
     1921 acccagctcg gtgccacggt cgtggccgaa gcgttgaagc gggccggcgt caagggtgac
     1981 gaggtcggcc atgtcgtctt cggccatgtg ctcaacaccg agccccggga catgtacctc
     2041 gggcgcgttg cggcgctcga tggtggtgtg tcgcaggaag cgccgtgcct gacgctcaat
     2101 cgcctgtgcg gcagtggcct gcaggcaatc atctccgcgg cacagaccat catgctcggg
     2161 gacgccgaca tcgccatcgg tggcggcgcg gaatgcatgt cgcgcggtcc ctacatcgtt
     2221 cccgcaggac gctggggcca gcgcatgggc gacatgaagg cgatcgacat gatggtgggc
     2281 gcgctgcacg acccgttcga cgtgatccac atgggtgtga ccgccgagaa cgtcgcaacg
     2341 aagtggggca tcacgcgaca ggagcaggat gcgctggcgg tcgagagcca tcgccgcgcc
     2401 gcagccgcga ccaaggccgg tcacttcaag agccagatcg tgccgatcga agtaaagggc
     2461 aagaagggcc cggtcgtgtt cgacaccgac gaacacttcc gcgagggcac gtcgatggac
     2521 gacatggcca agctgaagcc cgtgtttgca aaggaaaatg gcaccgtgac ggccggcaac
     2581 gcgtcgggca tcaacgacgg cgcgggcgcc gtcgtgctga tggaacgcag actcgctgaa
     2641 aagcgcggat tgaagccgct ggcacgcctg gtcagctacg gtcacgccgg tgtcgatccg
     2701 aagtacatgg gcatcggccc ggtgccatcg acgcgcaagg cgctcgagcg ggcagggctg
     2761 aaagtggccg atctcgatgt aatcgaggcc aatgaggcgt tcgccgcaca ggcctgtgcc
     2821 gtggccaagg accttggttt cccggcggac aaggtcaatc cgaacggcag cggaatctcc
     2881 ctaggccacc ccattggggc gaccggcgcg atcatcacca tcaaggcgct gtacgaactg
     2941 caacgggtcg gcggccgcta cgcgctcgtg acgatgtgca tcggcggcgg gcaggggatc
     3001 gctgcgatct tcgagcgaat ctgagccggg aaacggtccg gatggacgtc gatgcatcag
     3061 cctgtgggca ccggcacggc gagcggcgac gcctgcttat cgccgcgggg tgcgcgttgt
     3121 gcggcgccgt ctccctggtg cgtgcggcgc cggcgccaca gcagcccatg gacccgcggc
     3181 tggcggaact gctgaaggag caacgctctc tctgggttcg tcgtggcaag gaagaattgc
     3241 gcgcgaccta ctggaccgca gcgcagggac gcaaccagga cgaatatctg cagctttgct
     3301 ggatcatgcg tgacgtccag gccgatcgcg tgttcgccat ggaccggtct ttgctcgata
     3361 cgctagccgg aatccaggcg tggctgcagc ggtccggcgt caaccggccg atcgagctgc
     3421 attccggcta tcgcaccgga cggacgaacc gtgccactga aggggcagcg ctgagttcgc
     3481 ggcatgtcgt cgggcaggct gccgatatct cggtggcggg cgtgtcgagc gtcaagctgg
     3541 ccgggctgtc gagtgtgctc ggtcgcggcg gcaccgggtt ttatgtcggc cgcggattcg
     3601 ttcatgtcga caccggcaac gagcgcgtct ggatcgacca gaagcgcaag cccgcggcgc
     3661 cgaccggctg agtcaccgcg gcttgggttc cttgccgccg tctgttcagc gcggaccggg
     3721 tgcggtgatc ccggccgaac catgcgtgcg gtcgaggtcg atgtccagat acgacgccgg
     3781 gtcctcgcgc ggctcgatgt gggtggatac cgaagcgccg ggcacggccg ttgcgatttc
     3841 ctgctcgatc gattctgcga cgtcgtgcgc ctgctgcacc gtccaggcgc ccgggaccag
     3901 catgtgcatc gacacgaagg agcggccggc ggcggcgcgc gtgcgcagcg catggaactc
     3961 aatgtgctgc gcgcgatgcg cctgcagcac ggcttcgatt gccgcacgct gttcgccggg
     4021 cagcgcgcga tcgagcagcc cgagcgccga gcgacgcacg agcgagaagc cagtgaagac
     4081 aatgttgccg gcgaccgcca gggcgatcag cgggtcgagc cagttccaat gcgtgacggc
     4141 caccgcgccg acggcgatga cgacgccggc cgaggtccag acatcggtca tcaggtgatg
     4201 cgcatcggcc tcgagcgcaa tggaacggta acgccgtgcg gcgacgagca gcacgcgggc
     4261 caccgcgaag ttgatggccg ttgccaccag tgagagtccg atgccgagcc ccacgcgctc
     4321 gagcggctgg ggatgcagca gtcgatcgat ggcggcccat gcgatggcca gcgcggcggc
     4381 aaagatcaac gccccttcga gaccgctggc gaagtactcg gccttcgaat atccgtaggc
     4441 atgctcctcg gtcggcggac gcgccgacag cgagagcatc cacaaggtca tgccggccgc
     4501 aaccaggttc acgatcgact cgagcgcatc cgacagcagg ccgacggatc cggtcagcca
     4561 ccaggcgccg gtcttgatcg caatcgttgc gagcgcggcg gcaatcgaca gccacgcaaa
     4621 ccgggtcagg tgctcgcggc tcatcggcgc ggcgccgtgc ttcgaacgtc cgtggcgcgc
     4681 atcgctacgg cgaggccgag tcgccgaacc aagccagcac gccatcgagc ggcgcatggc
     4741 tcagtgcgaa cgtcgctttc tcgcgcacga cgggtttggc gcgccacgcg accgagatgc
     4801 cggccagcgc catcatcgtg aggtcgttgg cgccgtcacc gatcacgatc gcgcggtcgg
     4861 ttgcgcaggc gagttcggcg cactggtcac gcacggcctg cgcctttccc tcggcatcga
     4921 ggatatcgcc gcccgccggt ccggtcaccg tgccggtcag gcggccatcg acgattccca
     4981 gttcgttcga gcgcgttgca tcgaaaccca gccggcgctg gatgcgttcg gtgaagaagg
     5041 taaatccgcc agtgaccagc aacgctcgca ggcccgcagc gcgacaggtc gcaaccagag
     5101 gttcggcgcc gtcgttgagc gcaagcttct cgtcgtaaac ccgttgcagc agcgcggcat
     5161 cgactccggc cagcatcgcg acgcgccggc gcaggctctc ccggtagtcg gtgatttcgc
     5221 cgcgcatggc cgcttcggtg atcgcagcga cctcctcgcc cttgccggca taggccgcca
     5281 cttcgtcgag actttcgatc gtgatcagcg tggagtccat gtccagcgca agcaggcgga
     5341 agtccgacag gcgccaggcg ggatcgacga acgcgacgtc gagcttccag tagtcgccca
     5401 gtcctcgcac cgccttgcgc gactcgagat cgtcatgcac cgaaacgcag cgcgcggaac
     5461 gtcccgtcac ggtgattcgc tccggcatgc acacgacctt gaacgtatcg agcgcttcgg
     5521 gcgacaggga cggtccctgg atgacgaggt ctttcatgcg gcgcgaagtg tacgagtcag
     5581 ggttgcttca ggcgagcgcc ttgaagactt cgcgcagccg ctgcaggcgt gcctcgaggt
     5641 taggtgtctt ttcctcgatg cgcaggcgct cgggcccggt cagtctcatc gtccttgacc
     5701 gctgcatcag ggcgatcagc cgcgcggtgt cgaacggcgg attgggcacg aactgcagca
     5761 ccagcgcatc gccggatgcg tcgatcttct tcactccgag ccgctcggcg acgagccgca
     5821 ggcgatgcgt atcgatcagt gcgcgggccg gctccggcag cttgccgtaa cgatccacga
     5881 gttcctcctg caagtggatc aggccgtcct cgtcgtcgca gctcgcgagc ttcttgtaca
     5941 tcgacaggcg ctggtgcacg tcgttgacgt aatccggtgg cagcagcgcg ggcacgtgca
     6001 gattgatctc ggtgaccgcg gccagcggcg acagcaggtc gggctcgcgt cccgagcgca
     6061 gtgcccgcac ggcggcattg agcatctgtg tgtacagatc gaagccgacc tcctgcatgt
     6121 tgccgctctg gttctcgccc agcacttcgc cggcgccacg aatctccagg tcgtgcatgg
     6181 cgaggtaaaa accgctgccg agttcctcga ggttctggat cgcctcgagc cgcttctcgg
     6241 cattcttcgt gaggccgccc tcgtcgggca ccatcaggta ggcataggcc tggtgatgcg
     6301 agcggccgac gcgcccgcgc aactggtgca gctgcgccag cccgaacttg tcggcacggt
     6361 ggatgacgat ggtgttggcc gtcggcacat cgatgccggt ttcgatgatg gtggagcaca
     6421 gcaggacgtt gaaacgctgc tggtaaaaat cgcgcatgac gcgttccagg tcgcgctccg
     6481 gcatctggcc gtgcgcaatc tcgatgcgtg cctcgggcag gagctcagca agccgggcgc
     6541 gtcggttctc gatcgtctcg acctcgttgt gcaggaagta gacctggccg ccacgcttga
     6601 gttcgcgcaa cacagcctcg cgcatcaggc ttgccgactc cttgcgcacg aacgtcttga
     6661 tcgccagtcg tcgctgcggc gcggtggcaa tcaccgagaa atcgcggatg ccttcgagcg
     6721 acatggccag ggtgcgcggg atcggcgttg ccgtcagcgt caggacgtcc acttcggcgc
     6781 gtagtgactt cagccgctcc ttctgtctca cgacgaagcg gtgctcctcg tcgatgatga
     6841 ccagaccgag ccgctcgaac cggacgtccc cggacagcag cttgtgcgta ccgatgacga
     6901 tgtcgaccgt gccggcgttc atgccggcaa tcgccgcggc gacctccttg gtcgagcgga
     6961 agcgtgatag ctcgacgatg ttcacgggcc actgggaaaa gcggtcgcta aaggtctgga
     7021 agtgctgctc ggccagcagc gtcgtcgggc acagcacggc cacctgcttg ccgccggcca
     7081 ccgccgcgaa cgcggcccgc agtgccactt cggtcttgcc gaagcccacg tcaccgcaca
//
LOCUS       DATIRT010000219         4219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1604327, whole genome shotgun sequence.
ACCESSION   DATIRT010000219 DATIRT010000000
VERSION     DATIRT010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604327"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..164
                     /locus_tag="VLN25_05135"
     CDS             <1..164
                     /locus_tag="VLN25_05135"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSQ09984.1"
                     /translation="TVADVDETIRQAEKLGGAVVVPPMDVDGVGRMAVLRDPQGASFS
                     VIRYAFPSE"
     gene            complement(238..465)
                     /locus_tag="VLN25_05140"
     CDS             complement(238..465)
                     /locus_tag="VLN25_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09985.1"
                     /translation="MARKSATTMVALAAFGMIALTTALGVHGLQARQTAREVAALPVV
                     RMERVVIVGERTQAERPATTQVANNPTPTVR"
     gene            complement(700..1026)
                     /locus_tag="VLN25_05145"
     CDS             complement(700..1026)
                     /locus_tag="VLN25_05145"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HSQ09986.1"
                     /translation="MTFPIQITFHRMDRSEALERAIHEKAEKLGKFHPAITQLHVAVT
                     SETRHSSTAREYRVRLDVHVKNKEYAVTRQADEDVFVAARDAFDTARRVLDEDIDTRR
                     GFVKQH"
     gene            complement(1146..2204)
                     /gene="rsgA"
                     /locus_tag="VLN25_05150"
     CDS             complement(1146..2204)
                     /gene="rsgA"
                     /locus_tag="VLN25_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HSQ09987.1"
                     /translation="MTPEHQDRLRPIGYTPLIAQGLAQLDADGTAMRVKEVHRDSVVL
                     HDGDHERRGQAFPALAREFSLASESLAVGDWVLAQPDDYGDLWVAARMAPVTRIARRA
                     SDGARQCLVSNVDTALLVMGLDGDFNLRRLERYLAMVQGAGVWPVVVLTKRDLVASAN
                     AKTSDVIERVPADVPVLSVDARSAEVVSALTDYLGIGQTLVLLGSSGAGKSTLTNTLM
                     GRDVQATGAVRDDDSRGRHTTRSRSLHPLPDGACVIDTPGLRGLQVDIGEEGLGASFA
                     DIVALAEQCRFRDCSHTDEPDCAVRAHTHPDRLSNYHKLKREVRRETMTFLDRRKQLA
                     EWKARSRGAEQRMKMKRG"
     gene            complement(2526..2696)
                     /locus_tag="VLN25_05155"
     CDS             complement(2526..2696)
                     /locus_tag="VLN25_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09988.1"
                     /translation="MAKPNYSFEKRQRELAKKKKQEEKRQRKAADKPDKDAAPDSGAP
                     SVTPGDVPPATP"
     gene            complement(2789..2992)
                     /locus_tag="VLN25_05160"
     CDS             complement(2789..2992)
                     /locus_tag="VLN25_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HSQ09989.1"
                     /translation="MAQGTVKWFNESKGFGFITLEGGGKDLFAHFSEIQAAGFKTLQE
                     GQRVEFTVAQGAKGPQATQIRPI"
     gene            3301..>4219
                     /locus_tag="VLN25_05165"
     CDS             3301..>4219
                     /locus_tag="VLN25_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HSQ09990.1"
                     /translation="MQAILAVTVPFFALVLCGYLAGRARLLPEAAIPGLNIFVLYFAL
                     PCMLFRFGASLPVAQVINPVVLVITLIAAVMLVFATIALTRSDEVPMKDASLGALTVV
                     WPNTGFMGVPLLAALLGPAAAGPVITTLLVDLFFTSSLCIALAQAQDGNAGGTRAGVL
                     RLLKGTLANPLPAAIALGLLFSSLDLRLPGPLDTIVKMLGDAASPVALFAIGTVLWRA
                     GQHVHTRTPVRLFLPLALAKLVVHPLVVLGLGVVAIKLGFPLDSFTLTVIVLAAGLPS
                     ASSVSLLAERYGADNGRITRIIISSTVLAF"
BASE COUNT          683 a         1326 c         1397 g          813 t
ORIGIN      
        1 tgaccgttgc cgatgtcgac gaaacgatac ggcaggctga aaagctcggg ggcgccgtcg
       61 tcgtaccgcc tatggatgtc gacggtgtcg gccgcatggc ggtgctgcgc gatccgcagg
      121 gcgccagctt ttcggtgatc aggtacgcgt ttccgtctga gtaaaggtgc actgcgggca
      181 cgcggccggc gcgatcggtc cggccgcgta actgtccatt caagtcagcg aaccaagtca
      241 gcgaaccgtc ggtgtggggt tgttcgccac ctgtgtggtc gctggccgct ccgcctgcgt
      301 gcgttcgccg acaatgacca cgcgttccat ccggacgacg gggagggcgg cgacttcgcg
      361 cgccgtctgg cgtgcctgca ggccgtgcac acccagcgcc gtcgtaaggg cgatcatgcc
      421 gaatgcggca agggcaacca tcgtggtggc agatttccgg gccatggcgc gctccatctg
      481 aaagccgggc gacgaccgcg tcgcccacga gtgcactcta ggcagccccg ccctctgctt
      541 ccattcgatt gcgacgaact gcgagatttg cggcgccggc tgcaaaatgg cgcggcgcat
      601 ggagcgaagg ccattcgcgc cgcgatgaag ccgtgcgggc gccgacggac agcggcctcg
      661 tcactccgct gtcatcaatc gggcgacgtg cagcgcggat cagtgctgct tgacgaatcc
      721 tcggcgggta tcaatgtcct cgtcgagcac ccggcgcgcg gtgtcgaagg catctcgtgc
      781 agcgacgaag acgtcctcgt ccgcctggcg cgtgaccgcg tactccttgt tcttcacgtg
      841 cacatccagc cgcacccggt attcacgcgc cgtcgagctg tggcgggttt cagacgtcac
      901 cgcgacatgc aactgggtaa tggccggatg gaacttgccg agtttttcgg ccttttcgtg
      961 gatcgcccgt tcgagcgctt ccgaacggtc catgcggtga aacgtgatct ggatcgggaa
     1021 tgtcatggtc gtcacttcga tttggcaggg cacccgactt catggtggcg tgggcgccgc
     1081 ggcggacgtt gatggcggtc aacgcccgcg gctccctgac gcgcgatgcg agcccctgcc
     1141 gaagatcaac cgcgcttcat tttcattcgc tgttctgcgc cgcgactgcg agccttccac
     1201 tcggccagtt gcttgcgccg gtcgaggaag gtcatggttt cgcgccggac ttcgcgtttc
     1261 agcttgtgat agttcgacag gcggtccggg tgagtgtgcg cgcggaccgc gcagtcgggc
     1321 tcgtcggtat gcgagcagtc ccggaagcgg cactgttcgg ccagcgccac gatgtccgcg
     1381 aagctcgcgc cgagaccttc ttcgccgatg tcgacctgca ggccgcgcag tcccggcgta
     1441 tcgatcacgc aggcgccgtc cggcagtgga tgcagcgaac gcgaccgggt cgtatgacgg
     1501 ccgcggctgt cgtcgtcgcg taccgcgccg gtcgcctgga catcgcgccc cattaaagtg
     1561 ttggtcagcg tcgatttgcc ggcgccggaa gaccccagca atacgagggt ctgtccgatg
     1621 ccgagatagt ccgtcagagc cgataccacc tcggcggaac gcgcatcgac ggacagcacc
     1681 ggaacgtccg ccggcacccg ctcgatcacg tcggaggtct tcgcgttggc ggaagccaca
     1741 aggtcccgct tggtcaggac caccactggc cagactccgg ccccttggac catggccaga
     1801 tagcgctcga gccggcgcag gttgaagtcg ccgtcgagac ccatgacgag cagcgccgtg
     1861 tcgacgttgc tcacgaggca ctgccgcgcg ccgtccgaag cacgtctcgc aatccgcgtg
     1921 accggtgcca tgcgtgcggc gacccagagg tcgccgtagt cgtcgggctg tgccaggacc
     1981 cagtcgccga ccgccagcga ctcgctcgcg agcgagaact cgcgtgccag ggcggggaat
     2041 gcctgaccgc ggcgttcgtg atcaccatcg tgcagcacga cgctgtcgcg atgtacttcc
     2101 ttaacgcgca tcgcggtgcc gtccgcatcg agctgtgcga ggccttgagc gatgagcggg
     2161 gtgtagccga tcggtcgcaa ccgatcctgg tgctctggag tcattttcgt ttccgaatcg
     2221 agcgacgcga gggcgccgcg gacgcaacac gcgtgcaagc acgcgaccgg ggtcgatacg
     2281 tgttggccgg ccgctgcaag cggccgaatc ggaaacttcc gagtcagcgc cgagtcggcc
     2341 gactagcgaa ccagatcagt tttggacatg cgcgcctcct gaatcgagcg gcgctcaacg
     2401 agcggaaacg gaagtgttgc gccggagccg gcgcgcgtca accgtcacgt agcgcggtgg
     2461 ccgcgaggcg tgcgggccgc gcaacgtcgc ggccggcatt cgactggaat ctgacgcggt
     2521 cagtgtcagg gtgtggcggg aggcacgtcg cctggagtta cggacggtgc cccgctgtcg
     2581 ggcgctgcgt ccttgtcggg cttgtcggct gccttgcgct ggcgcttttc ttcctgcttt
     2641 ttcttctttg ccagctcgcg ctggcgtttt tcaaacgagt aattgggctt cgccaagtcg
     2701 gtgcctcctt aatgggatga gcgagtttcg cgcagataaa aaaagaccgc cacaaaaagt
     2761 ggcggtcgat ttctgaagcc aggaggcctt agatcgggcg gatctgcgtt gcctgggggc
     2821 ctttggcgcc ctgggcaacg gtgaactcca ctcgctggcc ttcctgcagg gtcttgaagc
     2881 ccgcagcctg aatctccgag aagtgggcaa acaggtcctt gccgccgcct tccagggtga
     2941 tgaagccgaa gcccttggac tcattgaacc acttgactgt accttgtgcc atttaaaacg
     3001 tccttgaaat gaaagatgga taactaacgc cggcaggaac gtcctgctgc gtgcaaacga
     3061 tcaagtacgt aaaccgggga acaaggcggg gtcggattaa acaatgacct gcacagggag
     3121 ccaatggact aacgacactt aaacgaaagc aatcgggagg ctacgcgctt gcgcgcgggt
     3181 tgtccagcac caccgcgaca tgcgcctgca gggtcggtag ggtgcggccg ggccgaggcc
     3241 gatggaagga cccggaaggc cctactgggg gaagaacgcc gtgcctgaga tacttgctcc
     3301 atgcaggcca tcctggcagt cacggttccg ttttttgcgc tggttctgtg cggctacctg
     3361 gccggccgcg cacgcctgct gcccgaggcc gccataccgg gcctcaatat tttcgtcctg
     3421 tactttgccc tgccgtgcat gctgttccgg ttcggcgcat cgctgccggt ggcacaggtg
     3481 ataaacccgg tggtgctcgt catcacgctg atcgccgcgg tcatgctggt gttcgcaacg
     3541 atcgctctga cgcggtccga tgaagtgccg atgaaggatg cgtcgctcgg tgcgttgacc
     3601 gtggtctggc cgaacactgg tttcatgggt gttccgctgc tcgcggcatt gctcggtccg
     3661 gccgccgcgg gtccggtgat cacaacgctg ctggtggatc ttttcttcac cagctcactg
     3721 tgcatcgcac tggcgcaggc gcaggatggc aacgcgggcg gcacgagggc gggagtgttg
     3781 cgcctactaa agggcacact ggccaacccc ttgcccgccg ccatcgcgct cggactgctg
     3841 ttttcgagtc tcgacctgcg actgccgggg ccgctcgaca cgatcgtcaa gatgctggga
     3901 gatgcggcgt ctccggtcgc acttttcgcc atcggcaccg tgctctggcg cgctgggcag
     3961 catgtgcaca cgcggacacc cgtgcgcctg ttcctgccgc tggctctcgc caagctggtc
     4021 gtgcatccgc tcgtcgtgct gggccttggc gtcgtggcca tcaagctggg attcccgctc
     4081 gattcgttca cgctgacggt gatcgtgctg gccgccggcc tgccgtccgc aagttcggtg
     4141 tcgctgctgg cggagcgcta tggtgctgac aacggtcgca tcacgcgcat catcatcagt
     4201 tcgaccgtgc tcgcgtttg
//
LOCUS       DATIRT010000220         2621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1506022, whole genome shotgun sequence.
ACCESSION   DATIRT010000220 DATIRT010000000
VERSION     DATIRT010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506022"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1266)
                     /locus_tag="VLN25_05170"
     CDS             complement(<1..1266)
                     /locus_tag="VLN25_05170"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HSQ09991.1"
                     /translation="MTKYVFVTGGVVSSLGKGIAAASLAAILESRGLKVTLIKLDPYI
                     NVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFISARMRKVNNFTTGQIYESVIKKE
                     RRGEYLGKTVQVIPHITNEIQDFVERGAAAAWGGKADVAIVEIGGTVGDIESLPFVEA
                     ARQMSLRLGRGSCCFVHLTLVPFIPMAGELKTKPTQHSVQKLREIGISPDVLLCRADR
                     PIPEEERAKISLFSNVPMNAVISVWDADSIYKVPSMLHKQGLDEIVCFTLGMPSRPAD
                     LTAWDKLVVALGNPQHEVRIGMIGKYVDLSDSYKSLNEALTHAGIHTHTRVVIDYVDS
                     EQIERDGIGALAQLDAILVPGGFGKRGTEGKINAIQFAREQGIPYLGICLGMQLATIE
                     FARHVCGLADANSTEFDPDTPHPVVALITE"
     gene            complement(1479..2453)
                     /locus_tag="VLN25_05175"
     CDS             complement(1479..2453)
                     /locus_tag="VLN25_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HSQ09992.1"
                     /translation="MNSRLLLACVGAMLYSSTGAFDLQAHRGGRGLAPENTRAAFEQA
                     LAIGVTTLEADLAMTRDGVLVLSHDPRLNPALTRSADGRWLDGTGPAIFSLTRSELAQ
                     FDVGRLNPESQYARQWPAQRPVDGSRIPTLAELFALAAGSAVRFNIETKITPEVARDG
                     VGDTPNAERFARAVVDEIRRAGLESRVTVQSFDWRTLVAVRQLAPEIVTACLTIQTAN
                     NDTVRPEADGASSWHAGLRLADHGGSVPRLVKAAGCAIWSPFWRNLSPGGVQEAHALG
                     LAVLPWTVNEPADLDRMVVLGVDGLITDYPDRARAVLEARGLPMPTAR"
BASE COUNT          462 a          860 c          834 g          465 t
ORIGIN      
        1 ctcggtaatc agcgcaacca ccgggtgcgg cgtatcggga tcgaactcgg tggaatttgc
       61 gtcggccagg ccacacacat ggcgggcaaa ctcaatcgtc gccagttgca tgcccaggca
      121 gatgcccagg tagggaatgc cttgctcccg tgcaaactgg atcgcattga tcttgccttc
      181 agtgccgcgc ttgccgaagc ccccgggaac gaggatcgca tcgagctggg ccagcgcgcc
      241 tatgccgtcg cgctcgatct gctccgagtc gacgtagtcg atcaccaccc gggtatgggt
      301 gtgaatcccg gcatgcgtca gcgcttcgtt caggctcttg tacgagtcgg aaaggtcgac
      361 gtacttgccg atcattccga tccgcacttc gtgctgcgga ttcccgagcg cgacgaccag
      421 cttgtcccac gccgtgaggt cggccggacg cgacggcatg ccgagcgtga agcagacgat
      481 ctcgtcgagg ccctgcttgt gcagcatcga gggcactttg tagatcgagt cggcatccca
      541 cacggagatg accgcattca tgggcacgtt ggaaaagagc gaaatctttg cgcgttcctc
      601 ctcgggaatc gggcggtcgg cgcggcacag cagcacgtcg ggcgaaatgc cgatctcgcg
      661 cagcttctgc acgctgtgct gcgtgggctt ggtcttcagt tcaccggcca tcggtatgaa
      721 cggaaccagt gtcaggtgaa cgaagcagca cgaaccgcgt ccgagcctca ggctcatctg
      781 ccgtgccgcc tcgacgaacg gcagcgactc gatgtcaccc acggtgccgc cgatctcgac
      841 gatcgcgaca tcggccttgc ctccccacgc cgccgcagcc ccacgctcga cgaaatcctg
      901 gatctcgttg gtgatgtgcg gaatgacctg cacggtcttg cccagatact ccccgcgtcg
      961 ctctttcttg atcaccgact cgtagatctg gccggtcgtg aagttattca ccttccgcat
     1021 ccgggcggag atgaagcgct catagtggcc gaggtccaga tcggtttcag cgccatcctc
     1081 ggtcacgaat acttcgccat gctggaacgg cgacatcgtg ccgggatcga cgttgatgta
     1141 cggatccagc ttgatgaggg tgactttgag gccgcgcgat tcgaggatcg cggcgaggga
     1201 ggcggcggcg atcccctttc ccagcgagga gaccacgcca ccggtaacga agacgtattt
     1261 cgtcattgat tctccgctcc gccccacgtt cagcacagcg cctcagcgag gttcgggcgt
     1321 taccggtaaa agcggattat accgatggcc cccgattgaa cccgtgacac ctgcacgttg
     1381 tgttgatatc ccgacgcacg gtctgttcgg tccagcgctg ttcacgcccg cagtcagcca
     1441 gcatgccttc acgtttcgcg gctcggaccg acgccggctc agcgggcggt gggcattggc
     1501 agtccacgcg cctcgagcac ggcgcgggca cggtcaggat agtcggtgat caggccatcg
     1561 acgcccagca ccaccatgcg atcgagatcc gcgggctcgt tgacggtcca cggcagcacg
     1621 gcaagtccga gcgcgtgggc ttcctggacc cctcctggcg agagattgcg ccagaacggc
     1681 gaccagatgg cacacccggc cgccttgacc agacgcggaa ccgagccgcc atggtcggcc
     1741 agtcgcaggc ccgcatgcca gctcgacgcg ccgtcggctt ccgggcgcac cgtgtcgttg
     1801 ttggcggtct ggatcgtcag gcaggcggtg acgatttccg gcgccagctg cctgacggcg
     1861 accagagtgc gccagtcaaa ggactgcacc gtcacgcgcg actcgaggcc ggcccgccgg
     1921 atctcatcga cgaccgcgcg agcaaagcgc tcggcattcg gcgtatcgcc gacgccatcg
     1981 cgggcgacct ccggcgtgat cttggtttcg atgttgaacc gtaccgcgct tccggccgcc
     2041 agtgcaaaga gttcggccaa cgtcggaata cgtgaaccat cgaccggacg ctgcgcgggc
     2101 cactgcctcg catactggct ctccggattc aggcggccga catcgaactg cgccagctcg
     2161 gagcgcgtca gcgagaaaat ggcaggtccg gtgccgtcga gccagcgacc gtccgcactt
     2221 cgtgtcagcg ccggattcag tcgcggatcg tgcgacagca ccagcacacc gtcacgcgtc
     2281 atcgccaggt ctgcctcgag cgtggtgact ccgatcgcca gcgcctgctc gaacgcagcc
     2341 cgcgtgttct cgggcgcaag gccacgcccg ccacggtgcg cctgcaggtc gaatgcgccc
     2401 gtactcgagt agagcatcgc acccacgcag gccaggagga gccgcgaatt catggtgcgc
     2461 acgatagccg cgatggtgcg cggcggggca gggagtcgga tacgagcctg cgatagactg
     2521 atttccgaag ggagtcctct cgatgcgtta ctgcccgacc tgcgggagtc cgatcaccct
     2581 gcgcgtgccg ggcggcgata cgcgggtacg tgcctgctgt g
//
LOCUS       DATIRT010000221         1935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1898931, whole genome shotgun sequence.
ACCESSION   DATIRT010000221 DATIRT010000000
VERSION     DATIRT010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1898931"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..173
                     /locus_tag="VLN25_05180"
     CDS             <1..173
                     /locus_tag="VLN25_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HSQ09993.1"
                     /translation="SVYVGDDLRDVQAGRAAGLRTVAAAYGYLGESDPVEQWGADHII
                     HAPDELLRLIRQ"
     gene            257..619
                     /gene="ssrA"
                     /locus_tag="VLN25_05185"
     tmRNA           257..619
                     /gene="ssrA"
                     /locus_tag="VLN25_05185"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            905..1156
                     /locus_tag="VLN25_05190"
     CDS             905..1156
                     /locus_tag="VLN25_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09994.1"
                     /translation="MSATANVWDLSPWMWFIAGQSRVIDLSLTLPPDVVRGGAFGLVS
                     GSAPLPLGFSLQASGLLTVVDPAVGQTANVQFTYAEPGT"
     gene            complement(1131..1589)
                     /locus_tag="VLN25_05195"
     CDS             complement(1131..1589)
                     /locus_tag="VLN25_05195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HSQ09995.1"
                     /translation="MKRCIAVLIAGAGLAACAPAQAEVFYSWVDVAQKPKLATTGDTL
                     MRKQLCARSEVDGSCLDGGPSAKDKLLLPTEPPELGDPPPVITLPPPVVEELPPVVNS
                     TPPAPISEEPTTTPVPEPTTLTLLAAALLGLVAGKRRHRGRAVTCPVLRT"
BASE COUNT          369 a          602 c          580 g          384 t
ORIGIN      
        1 agtccgtcta cgtaggtgac gacctgcgcg atgtacaggc gggccgagct gccggtttac
       61 gtacggtggc cgcggcctat ggctatctcg gtgagtcaga cccggtcgaa caatggggtg
      121 ccgatcacat catccatgcc ccggacgaac tgctccgcct gatccggcaa tagcgtcctc
      181 gcaaagactc caaagcgaaa actcgggttg atttcccgct gaagcgaccc acattgatcg
      241 tagaattact tttcttgggg gcgacctggc ttcgacacgg gtcgtgaagg atctcagggc
      301 atgccgagga ccagtgacct cgtaaatcca tctggaacac tacaaacgcc aacgaagacg
      361 cgtttgctct agccgcttaa taccggccag acgctgcact ggttcgcttc cgggccaggt
      421 cgggtaaccg acagcagcga cattcacgga agctggatgc cggtggagtc acgacggcgg
      481 catcgagacc cagtgactgg ctgcgcgccg gcctgccgat cggctaagca cgcggcgaga
      541 caaaaataga ccggctacgc atgtagaact ggatctggat cactcgtgga cgcgggttca
      601 attcccgccg cctccaccaa caaacgcaaa gggccgccac cagcggccct ttgccgtggg
      661 acacggacga cgcctcggcg attcggcagc accccttcaa atctctcgac gatcaatggc
      721 agcaaccggg acttcgttca cgatgaagcg caggtcgctg ctgctgggtg ccaccggttt
      781 cggcggccta cgtttcagcg gactacgttt cagccgccta ggtttcagcg gcctaggttt
      841 cagcggattg gctctgtacg gatgcggagg ttcgggcgca tcacccaccg ctgcccctgc
      901 ggcgatgagc gcaacggcca atgtatggga tctgtctccc tggatgtggt tcatcgcggg
      961 acagagccgg gtgatcgacc tatccctgac gctgcccccg gacgtcgtcc gcggtggtgc
     1021 gttcggactc gtcagcggca gtgcgcccct gccactgggt ttctcacttc aagcgtccgg
     1081 actgcttacc gtcgtcgatc cggctgtcgg acagactgcc aacgtgcagt tcacgtacgc
     1141 agaaccgggc acgtgacagc ccgaccgcga tgccgcctct ttcctgccac gaggccgagc
     1201 aacgccgcgg caaggagcgt cagcgttgtc ggctcgggca cgggtgtcgt ggtgggctct
     1261 tccgagatcg gtgccggtgg tgtgctgttg accaccggcg gcaactcctc gacgaccggc
     1321 ggaggcagcg taattacggg cgggggatcg cccagttccg gcggctcggt cggcagcaga
     1381 agtttgtctt tggccgacgg cccgccgtca aggcaggaac cgtcgacttc gctgcgcgcg
     1441 cacagctgct tgcgcatcag cgtgtcaccc gtcgtcgcaa gcttgggctt ctgcgcaaca
     1501 tcgacccagc tatagaacac ctctgcctga gcgggagcac atgcagccag cccggctccg
     1561 gcaatgagta cagcgataca gcgtttcatc aacagcccct tggcgccgta gtaccggcgc
     1621 acaactgatt tctacatccc caaattacaa tggtcgagtc actaaatcga tcgtctatgt
     1681 agacgatcaa tgttgtgccg tatagcgacc gtggacctgt caggtttttc gacacttgtg
     1741 cgcctttcag catgacctgg cagttttcat caatgcgggt tcgatcgcgt ttgaaacgtg
     1801 gtaactacta tggccgatag caccgatatc ccgactctgc gccgactgct gcacgattgc
     1861 cgtcgaattg ccgtcgtcgg actctcggca gagtggcatc gcccaagtta cttcgtcggc
     1921 aagtacctgc tcgag
//
LOCUS       DATIRT010000222         6689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_424994, whole genome shotgun sequence.
ACCESSION   DATIRT010000222 DATIRT010000000
VERSION     DATIRT010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6689
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424994"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1827)
                     /locus_tag="VLN25_05200"
     CDS             complement(28..1827)
                     /locus_tag="VLN25_05200"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF003220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HSQ09996.1"
                     /translation="MTTSPRGAHARIYSISGPVLKARAQGPFVLREAIRVGPAALLGE
                     VIRLNEDEIVAQVYEDTAGLRPGVEVTGTGDLLGIQVGPGLLGNIFDGLLRPLTPPVQ
                     QIFEFTPGVAAGTQVAAGSVFGTLTSTGGRPQRALVPPTVAGTVEWTAAAGAVADDAT
                     VLRIRSDAGDEHALSLRHFWPVRTPRPVRGRLASDEPLVTGQRILDSLFPVALGGKGA
                     IPGGFGTGKTILLEALAKGCSADVIVYLGCGERGNEMASVLEEFPQLVDPRTGRSLME
                     RTVIIANTSNMPVAAREASIYSAVTVAEYFRDQGLHVALMADSTSRWAEALREVSGRF
                     GELPGEGGYPTYLSSRLADFYERAAKVTTLNGDIGSVTLLGSISPPSGDFSEPVTSHS
                     KRYVRSFWALDAKRAQARFYPAVNPLQSYSEDALDVAVWWLKRYAAVPTARARAASGE
                     NEWLELRRRFLTLLDEQARLERMARIIGKDALPARQRLTLMCAELINEAFLRQSAFSE
                     IDRYCGPDRQACMMRLLVHFIELAESATAFGVTPEQIAALDVMRPLSRMGEDIGENEL
                     ERLAELERRVEQAFATLVVSSAPAQVGDDTHAA"
     gene            complement(1904..2521)
                     /locus_tag="VLN25_05205"
     CDS             complement(1904..2521)
                     /locus_tag="VLN25_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ09997.1"
                     /translation="MNLDDRLKALLELVEQFRARRHAELLEPARADARETIRSALAES
                     RRRVRTAIAEERKRYAAEVGAVEAALATDRRLASQRHSVRLLGDAWHTLRERLVARWD
                     DASTRAEWITAHLDRALKAVPRHESGWQIEHHPAWSEPERGHARERLRVDGVTPVQFE
                     ATAGIAAGFRVVSGHNVLDATLEGLVADRTQLEGRLLHHLEPEPH"
     gene            complement(2532..2825)
                     /locus_tag="VLN25_05210"
     CDS             complement(2532..2825)
                     /locus_tag="VLN25_05210"
                     /inference="COORDINATES: protein motif:HMM:NF014092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HSQ09998.1"
                     /translation="MGAFAYVGNAVDAAGYRLAGVPSWAPDRGAELAAFRAARASAEA
                     VFITFAVAEHLPREELDAALAGDRPVVVLIPESDGASPLEPAERVRAQLGLEP"
     gene            complement(2848..3195)
                     /locus_tag="VLN25_05215"
     CDS             complement(2848..3195)
                     /locus_tag="VLN25_05215"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HSQ09999.1"
                     /translation="MRRFALPLLLIAGAAALAALGTVMLVFPDTASAQSAARTALPPD
                     AWTWGLVSAALATAASSIAGGYAVAKLGAAAVGALAEKPDLFGRLLIFVGLAEGIAIY
                     GVIISILILNRLT"
     gene            complement(3205..5004)
                     /locus_tag="VLN25_05220"
     CDS             complement(3205..5004)
                     /locus_tag="VLN25_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10000.1"
                     /translation="MIRPQPCSWFEIMVARDDAFIALEALAAAGCIEVEWHQGEAPGA
                     AMPQDLLKSYNAMARKFRPYWPRSATILEAERRAPADALSSGVSVLQAWATAAEDTIV
                     RLQQAEAEAAELELAATALREMADSRIDFSALARADHGVTAALFALPLGVEVDVPEDT
                     ITRFAALATEQLLLVIGPPEAVDRMGRTVAEVNGRRARFPDWLQPSAAANLSMVAERR
                     AERQAVIAGLRAEVNTLSQQHGVARALGTIARATWCFEHGGAIDEGDVFARITGWTAD
                     REQVVRALEGSDARGLATFPRPPRGVRAPLVLRNPWWAQPFEVFTRLVGMPSASGADP
                     SVLLALAVPLIFGYMFGDVGQGLVLAAVGWLLRKRLPVLRLLVPGGLAAMLFGFVFGA
                     VFTLEHVIEPWWLHPIEHPLPVLLVPIFGGAALLALGLLLGALEAWWESRFDHWLRDD
                     VPVLLALMGALAAFLDPMAWWLVLAAAAWALVAGALDKHSAKAALSALGEWVEHTIQI
                     LINTLSFARVGAFALAHAGLSSAVVALASAGGNRFAYVLILIIGNVLILVVEGLVVSI
                     QTTRLVLFEFFTRFFRPEGREFRPLAAPPVTLH"
     gene            complement(5001..5831)
                     /locus_tag="VLN25_05225"
     CDS             complement(5001..5831)
                     /locus_tag="VLN25_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10001.1"
                     /translation="MISGLTLEYAYARVAARIAQRPDERLWLQLRSARTVPALLEAVR
                     ASAAASTVSGIPLTGDAEVIEHAFRQQLRTRIDEVASWSPEAWQPAVHYTRHLVDLPA
                     IAHLLTDEPPPRWMGVDPALSTYVHAGRTDRRAALLAGPLAPITRAIEEETPARAATE
                     PLARALRRVRAGPPIHRAIAAWESEWRARWPRAASDLASALDDVVHAARRHVLSFATL
                     IPDDTAAARQSLAARLIALLHRAAAQPAALFAYLALFALDVERLRGEFVVRARLGTPP
                     "
     gene            complement(5828..6139)
                     /locus_tag="VLN25_05230"
     CDS             complement(5828..6139)
                     /locus_tag="VLN25_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10002.1"
                     /translation="MDEAIVRVLEAEQAARAAVVQYTSDGDEIRAQARLRAHAIAERA
                     AGRVARVHRWTDVAIRQRVDQLNGERSALHAAEPLDPDEPARVARALDRLAAELSGGT
                     E"
     gene            complement(6325..>6689)
                     /locus_tag="VLN25_05235"
     CDS             complement(6325..>6689)
                     /locus_tag="VLN25_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HSQ10003.1"
                     /translation="LMYPTTTISFIGLRSGGRGEPPHVIHCCGDNSRYDPLHGARVID
                     GPAPQPLAAVLLEWDAASDRLYAVGTRGGEMFDAFFEKYAAKLELETGSGLRKPSGPT
                     VAVQPAASYSRLWRSCRA"
BASE COUNT         1129 a         2273 c         2303 g          984 t
ORIGIN      
        1 cgcatggccg catcgtgcac ggtgactcta ggctgcatgc gtgtcgtccc caacctgtgc
       61 cggagcgcta ctgaccacga gcgtggcgaa cgcctgttcg acgcggcgct cgagttcggc
      121 cagacgttcg agctcgttct cgccgatgtc ctcgcccatg cgcgacagtg gccgcataac
      181 atcaagtgca gcgatttgct ccggagtgac gccgaatgcg gtggccgatt cggccagttc
      241 gatgaagtgc accagcagcc gcatcatgca ggcctgcctg tccggtccgc agtaccgatc
      301 gatctccgag aacgccgact gacgcagaaa cgcttcgttg atcagctcgg cgcacatcag
      361 cgtgagacgc tggcgcgcag gaagagcatc cttgccaatg atgcgggcca tgcgctcgag
      421 ccgcgcctgc tcgtcgagca aggtcaggaa tcgccgccgc agttcgagcc attcattctc
      481 gcccgaggca gcgcgggcgc gcgccgtcgg cacggctgca tagcgcttca gccaccagac
      541 cgcaacgtcg agggcgtcct cggaatacga ctgcagcgga ttgaccgccg gatagaagcg
      601 cgcctgcgca cgcttcgcat cgagtgccca gaagctgcgc acgtagcgct tgctgtggct
      661 cgtcaccggc tcggagaagt cgcccgaggg cgggctgatc gagccgagca gggtgaccga
      721 gccgatgtcg ccgttgagcg tcgtgacctt cgcagcgcgt tcgtagaagt cggccaggcg
      781 gctcgacaga taggtcggat agccgccttc gcccggcagt tcaccgaagc ggcccgacac
      841 ctcgcgcagg gcctcggccc agcgcgaggt cgaatccgcc atcagcgcga cgtgcaggcc
      901 ctggtcgcgg aagtactcgg caacggtcac cgcggagtag atgctggcct cgcgcgcggc
      961 caccggcatg ttcgacgtgt tggcgatgat gaccgtgcgt tccatcagcg aacgcccggt
     1021 gcgcgggtcc acgagctgcg ggaactcttc gagcacgctc gccatttcgt tgccgcgttc
     1081 gccgcaaccg agatagacga tcacgtcggc gctgcagccc ttggcgagcg cctccagcag
     1141 gatggtcttg ccggtgccga agccgccggg gatcgccccc ttgccgccca gagcgacggg
     1201 aaacagggag tcgaggatcc gctggcccgt gacgaggggc tcatccgagg ccagtcgccc
     1261 gcgcaccggc cggggtgtgc gcaccggcca gaagtgccgc agcgacaggg catgctcgtc
     1321 tcccgcgtcg cttctaatac gcaggacggt tgcatcgtcg gcaaccgcgc cggcggcagc
     1381 tgtccattcg acggtgcccg cgacagtcgg cggcacgagt gcgcgctgag gtcgaccccc
     1441 tgtcgatgtc agtgttccga agacggatcc ggcggcgacc tgtgtgccgg cagcaacgcc
     1501 aggcgtgaac tcgaagatct gctgcaccgg cggcgtcagc gggcgcagca ggccgtcgaa
     1561 gatgttgccg agcagaccgg gtccgacctg gatgccgagc aggtcgccgg taccggtaac
     1621 ttcgacgccc ggtcgcagtc cggcggtgtc ctcgtagacc tgcgcgacga tctcgtcctc
     1681 gttcagccgg atcacttcgc cgagcagggc tgcggggccg acacggatcg cctcgcgcag
     1741 cacgaacggc ccctgcgccc gggccttcag caccggaccg ctgatcgaat aaatgcgggc
     1801 gtgtgcgccg cgtggcgacg tggtcatgcg acctccgtca ttgcccggtc cgatgcggcg
     1861 aacggtgcga ggcaaggcga ccgacggggg acgatccatg acatcagtgc ggttccggtt
     1921 cgaggtgatg tagcagccgg ccttcgagct gcgtgcggtc cgcgaccagg ccttcgagtg
     1981 ttgcgtcgag gacgttgtgg ccgctgacaa cgcggaagcc tgccgcgatt ccggcggtgg
     2041 cctcgaactg caccggcgtc actccgtcga cgcgcaggcg ctcgcgggca tggccacgct
     2101 ccggctcgct ccaggctgga tgatgttcga tctgccagcc cgactcgtga cggggcaccg
     2161 ccttgagagc acggtccaga tgagccgtga tccactcggc gcgtgtcgag gcgtcgtccc
     2221 agcgcgcgac cagacgctct cgcaatgtgt gccacgcgtc gccgagcagg cgcaccgagt
     2281 ggcgctggct ggcgagacgg cgatccgtgg ccagcgcagc ctcgaccgcg cccacttcgg
     2341 cggcgtagcg cttgcgctcc tctgcgatgg ccgtgcgaac gcgccgacgc gactcggcca
     2401 gcgccgagcg tatcgtctcg cgcgcatctg cacgcgccgg ctcgagcagt tcggcatgcc
     2461 gacgcgcacg gaactgttcg accagctcca gcagcgcctt caagcgatcg tcgaggttca
     2521 tgcgccgttg ctcaaggttc gagtccgagt tgcgcacgta cgcgctccgc cggctcgagc
     2581 gggctcgcgc cgtcggattc cggaatcagc acgacgacgg ggcggtcgcc tgcgagcgcg
     2641 gcatccagtt cctcgcgcgg cagatgctca gccacggcga aggtgatgaa cacggcctcc
     2701 gcgctcgctc gcgcggcccg gaacgcggcg agctcggcac cgcggtcggg cgcccacgac
     2761 ggcacaccgg ccagccggta tcccgccgcg tccacggcat tgccgacgta tgcgaacgcg
     2821 cccatcgatc agatccagcg gtgcagctca ggtcaagcgg ttgaggatca ggatcgagat
     2881 gatcacgcca tagatggcga tcccctccgc caggccgacg aagatcagca gtcgtccaaa
     2941 caggtccggc ttttccgcca gcgctccgac ggcggcggcg ccaagcttgg ccactgcgta
     3001 tccgccggcg atcgacgatg cggctgtggc cagtgctgcc gacacgagac cccatgtcca
     3061 cgcatcggga ggaagggccg tccgagcggc cgactgcgcc gaggcggtat cgggaaacac
     3121 gagcattacc gtgccaagcg cggcgagcgc ggctgcgccg gcgatcagca gcagcggtaa
     3181 agcgaagcga cgcatgatga ctcctcagtg aagcgtgacg ggtggagcag cgagtggtcg
     3241 aaactcgcgc ccctcgggac gaaagaaccg ggtgaagaac tcgaacagga cgagacgcgt
     3301 ggtctggatc gacacgacga gtccttcgac gaccaggatc agcacgttgc cgatgatcag
     3361 gatcagcacg taggcgaagc ggtttccgcc cgcagaggcg agagcaacga cggccgatga
     3421 cagcccggcg tgcgcgagcg cgaaggcgcc gacgcgcgcg aacgacaggg tattgatcag
     3481 tatctgaatc gtgtgttcga cccactcgcc aagggcgctc agcgccgcct ttgcgctgtg
     3541 cttgtcgagt gcgccggcca cgagtgccca cgcggccgcg gcgagaacca accaccatgc
     3601 cattggatcg aggaatgctg ccagtgcacc catgagggcg agcagcaccg gcacgtcgtc
     3661 gcgcaaccag tgatcgaagc ggctctccca ccacgcttcg agcgcgccga gcagcaggcc
     3721 cagtgcaagc agggcagcac cgccgaagat tggtacaagc agtacaggca acgggtgctc
     3781 gatcggatgc aaccaccagg gctcgatcac gtgctccagc gtgaacaccg caccgaacac
     3841 gaagccgaag agcatcgcgg caagaccacc ggggaccagc agccgcagca cgggcaggcg
     3901 tttgcgcaag agccagccca cggctgccag taccagcccc tggccaacgt caccgaacat
     3961 gtagccgaag atcagcggca cggcgagcgc cagcagtacg ctcggatcgg cgccgctcgc
     4021 gctgggcatg ccgacgagtc gcgtgaacac ttcgaacggc tgcgcccacc acggattgcg
     4081 cagcaccagc ggcgcgcgca cgccgcgcgg cggacgcgga aaggtggcca ggccccgggc
     4141 gtcgctcccc tcgagtgccc gcacgacctg ctcgcggtcg gcggtccagc cagtaatgcg
     4201 cgcaaacacg tcaccttcat cgatcgcgcc accgtgctcg aagcaccagg tggcgcgcgc
     4261 aatggtgccc agcgcacgcg caacgccatg ctgctgcgac aacgtattga cctcggcgcg
     4321 cagcccggcg atcacggcct ggcgttcggc gcgtcgctcg gcgaccatcg acaggttggc
     4381 tgctgcactc ggttgcagcc agtccggaaa gcgggcgcgg cggccgttga cttcagcgac
     4441 agtgcgaccc atccggtcga cggcctcggg cggaccgatc accagcagca gttgctccgt
     4501 cgcgagtgct gcgaagcgcg tgatcgtgtc ctcgggaaca tcgacctcga cgcctagggg
     4561 aagtgcgaac agggccgcgg tcacgccgtg gtcggcacgc gccagtgcag aaaagtcgat
     4621 gcgactgtcg gccatttcgc gcagcgcggt ggcggccagt tcgagctcgg ccgcttccgc
     4681 ttcggcctgc tgcagccgga cgatcgtgtc ctcggcggcc gttgcccagg cctgaagcac
     4741 cgacactccg gaggacaacg cgtcggcggg cgcacgacgt tctgcctcga ggatggtcgc
     4801 ggacctgggc cagtagggtc ggaacttgcg ggccatcgcg ttgtacgact tcagcaggtc
     4861 ctgcggcatg gcggcgcccg gcgcttcgcc ctgatgccat tcgacttcga tacagccggc
     4921 ggctgcgagc gcttcaagcg caatgaacgc atcgtcccgc gcgaccatga tctcgaacca
     4981 ggagcagggc tgcggacgga tcatggcggt gtgcccagtc gcgctcggac gacgaactcg
     5041 ccgcgcagcc gctcgacgtc gagcgcaaag agcgcgagat aggcgaagag cgccgcgggc
     5101 tgggctgctg cccgatgcag cagcgcaatc aggcgcgcag cgaggctctg ccgcgcggcg
     5161 gctgtgtcgt ccgggatcaa cgttgcgaaa gacaggacgt gccgccgcgc ggcatgaact
     5221 acgtcatcga gggccgatgc caggtcgctg gccgcacgcg gccaccgggc acgccattcg
     5281 ctttcccagg ccgcgatcgc gcggtggatc ggcggccccg cccggacgcg gcgcagcgcg
     5341 cgtgcaagcg gttcggtcgc cgcacgggcc ggagtttcct cctcgatggc gcgggtgatc
     5401 ggtgccagtg ggccagcgag gagcgcagcg cgacgatccg tgcggccggc gtggacgtaa
     5461 gttgagagcg ccggatcgac ccccatccat cgaggcggtg gctcgtccgt cagcaggtgt
     5521 gcgatggcag gaaggtcaac gagatggcgt gtgtaatgca ccgcgggctg ccacgcctcc
     5581 ggcgaccagg aggcgacctc gtcgatgcgt gtacggagct gctgccggaa ggcatgctcg
     5641 atgacctcgg cgtcgccggt cagcggaatg cccgagaccg tggaagcggc ggccgatgca
     5701 cgaaccgcct cgagcaacgc gggcacggtc cgcgccgagc gcagctgcag ccagagtctt
     5761 tcgtccgggc gttgggcaat ccgcgcggcc acccgcgcat acgcgtactc gagcgtcagg
     5821 ccggagatca ttccgtgccg ccactgagtt ccgccgcgag ccggtcgagc gcgcgcgcca
     5881 cgcgtgccgg ctcgtcggga tcgagcggtt cagcggcgtg cagcgctgac cgttcaccat
     5941 tcagctgatc gacgcgttga cggatcgcga catcggtcca gcggtggacg cgggcgacgc
     6001 gcccggcggc gcgctcggca atcgcatgag cgcggagtcg tgcctgcgcc cggatctcat
     6061 cgccatccga cgtgtactga acgacggccg cgcgagcagc ctgctccgcc tcaaggaccc
     6121 gtacgatcgc ctcgtccacg cgtgcagcgg acgtggtatc cgcatgtgca gccactgctc
     6181 gccccaatgc acagaggcag gcgcccgcaa cccggcccca tcctctggcc agccctttta
     6241 tacggggggt gtgttcaggc gaccttgcgt cagagcaatc ggtcggcgat cagggcgatt
     6301 gctaccaact gtcgtctagc agggtcatgc ccggcagctg cgccacagcc tgctgtaact
     6361 cgcagccggc tgcacggcga cggtgggacc gctgggcttg cgcaggccgc taccggtctc
     6421 gagctccagt tttgccgcgt acttttcaaa gaaggcgtcg aacatctcgc cgcctcgcgt
     6481 gccgaccgcg tacagccggt cgctggcggc gtcccactcc agcagcacgg cagccagtgg
     6541 ctgcggcgcc ggcccgtcga tcacgcgcgc gccgtgcaag gggtcgtagc gggagttgtc
     6601 gccgcagcag tggatcacgt gcggcggctc accgcggccg ccgctgcgca gtccgatgaa
     6661 gctgatcgtc gtcgtggggt acatcagtt
//
LOCUS       DATIRT010000223         1823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1605002, whole genome shotgun sequence.
ACCESSION   DATIRT010000223 DATIRT010000000
VERSION     DATIRT010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1605002"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VLN25_05240"
     CDS             complement(<1..282)
                     /locus_tag="VLN25_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HSQ10004.1"
                     /translation="MNRRTIPASRPRRATSGPRIGLALAGGGPLGAIYEIGALAALTE
                     ALEGIDFNDVDVYVGVSAGGFIAAGLANGITPHQMSRLFIEGEKGRDRFE"
     gene            complement(421..1080)
                     /locus_tag="VLN25_05245"
     CDS             complement(421..1080)
                     /locus_tag="VLN25_05245"
                     /inference="COORDINATES: protein motif:HMM:NF017415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HSQ10005.1"
                     /translation="MSGGLRRGATLSRAALRASIRIAAQRISRIEREQALEPKLYAVC
                     CRAHAVTPRLQTSENTSARQGVEMVKKLKAMAEKSHSESQLRAAVKDSAQEIWLAGLG
                     AFAKAQEEGQKVFKALVREGTSIQKRTMKVTEDKVNDVTAKVTKVAAGLQKQANGTWD
                     KLETVFEARVERALTRLGVPTNKEIAALTKRVEELTRSVGSLTKSRPAKAPAKRARKA
                     A"
     gene            1331..>1823
                     /locus_tag="VLN25_05250"
     CDS             1331..>1823
                     /locus_tag="VLN25_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSQ10006.1"
                     /translation="MTYFVTGATGFIGKRLVKKLLARRATTVYFLVRKESLDKVPALL
                     EYWETSEERAVPVVGDLGKAMLGVTKADQKKLTGKVKHFFHLAAVYDLKADAESQIVA
                     NIDGTKNAVALANEIKAGVFHHVSSIAAAGLYEGVFREDMFDEAENLDHPYFATKHDS
                     EKIA"
BASE COUNT          312 a          597 c          577 g          337 t
ORIGIN      
        1 ctcaaaccgg tcgcggcctt tttcaccctc gatgaacagg cgcgacatct ggtgcggcgt
       61 gatgccgttg gccaggccgg ccgcgatgaa gccgccggca ctgacgccaa cgtagacatc
      121 gacatcgttg aagtcgatgc cttcgagtgc ctcggtcagt gccgccaggg caccgatctc
      181 gtagatggct cccagaggac ctccgccagc cagagcaagg ccgatgcgcg gacccgacgt
      241 cgcgcgtcgc gggcgggaag cgggaatggt gcgacggttc atggcgccct tagggggcga
      301 ggcgatgtgg ccgtcctcat gctgccgatc gtcacggccg gttgcccgct ggctccgatg
      361 cacgcccagc gggtgtcgac cttgggccgc gcgcaggcgc gaccacggtg tcgaaggtga
      421 tcaggccgcc ttgcgagcgc gcttggccgg agccttggcc ggacgcgact tggtcagcga
      481 gccgacgctg cgggtcagct cctcgacgcg cttcgtcagc gcggcgatct ccttgttcgt
      541 cggaacgccg aggcgcgtca gagcccgctc gacacgcgct tcgaacaccg tctcgagctt
      601 gtcccaggtg ccgttggcct gcttctgcag tccggctgcc actttcgtca cttttgccgt
      661 cacgtcgttg accttgtctt cggtgacctt catggtccgc ttctgaatgc tggtgccctc
      721 gcgaaccagc gccttgaaca ctttctggcc ctcttcctgc gccttggcaa acgcgccgag
      781 gccggcgagc cagatttcct gtgcagaatc cttcaccgcg gcgcgcagct ggctttcgct
      841 gtgcgacttc tcggccattg ccttgagttt cttgaccatc tcgactcctt gcctcgcgga
      901 agtattttcc gatgtctgaa gtctaggagt caccgcgtgc gcccgacaac aaactgcata
      961 gagcttcggc tctagggcct gctcacgttc aatgcgggag atgcgttggg ccgcaatgcg
     1021 gatggatgcg cgaagggcgg cgcggctcag ggtggctccc ctgcgcaagc cgcccgacaa
     1081 agcagacgcc gcatttcggc ccaacccgga gggaaggcga atggcgggcg tattgcgtcc
     1141 gcacttgcca atgagcgctt cgcttgttgg catcagccaa tggcgctcgc ggcgcctcgc
     1201 actgcctccc gccattcgcc ttcgcactcc cggcttgagc gtgaacaggc cctggcgcat
     1261 gcggcgctgg cgctacactg ccgcgagatt cgtagagcga acacaccatc ctgccaggag
     1321 gagacatccg atgacgtact tcgtcaccgg cgcgaccggt ttcatcggca agcgcctcgt
     1381 caagaaactg ctcgcccgcc gcgccacgac ggtctacttc ctcgtgcgca aggagagcct
     1441 tgacaaggtg ccggcgctgc ttgagtactg ggagacgtcc gaagaacgcg ccgtgccggt
     1501 cgtcggggac ctgggcaagg cgatgcttgg agtgacaaag gcggaccaga agaaactgac
     1561 gggcaaggtc aagcatttct tccacctggc ggccgtctac gacctgaagg cggacgcaga
     1621 gagccagatc gtcgccaaca tcgatggaac gaaaaacgcg gtggcgctcg ccaacgaaat
     1681 caaggccggc gtgttccacc acgtttcgtc gatcgccgcg gccggcttgt acgagggcgt
     1741 gttccgtgag gacatgttcg acgaggccga gaacctcgat cacccgtatt tcgccaccaa
     1801 gcacgactcc gaaaagatcg ccc
//
LOCUS       DATIRT010000224         2613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1310108, whole genome shotgun sequence.
ACCESSION   DATIRT010000224 DATIRT010000000
VERSION     DATIRT010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310108"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="VLN25_05255"
     CDS             complement(<1..162)
                     /locus_tag="VLN25_05255"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HSQ10007.1"
                     /translation="MKEFILEVPLRWGDMDAMAHLNNVMYFRLMEEARIQWFEQFGFS
                     TLPTGEAPIL"
     gene            complement(182..943)
                     /locus_tag="VLN25_05260"
     CDS             complement(182..943)
                     /locus_tag="VLN25_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSQ10008.1"
                     /translation="MRTNLKEKVALVTGASSGLGARFARVLASNGALTVLASRRLERL
                     KDLRAEIEAAGGAAHVVQLDVTDLNSIREAVEHTERAVGPIDILVNNSGVSTTQRLTD
                     ATLADFDHVFATNVKGAFFVAQEVAQRMIARSKSGPGFRGRIVNIASVAGLRVLPQIG
                     LYCMSKAAVIQMTRAQALEWGRYGINVNAICPGYIRTEINDEYWETEGGRKLIQLLPR
                     RRIGQPEDLDGTLLLLVAEESQFINGAIIAADDGM"
     gene            1053..>2613
                     /locus_tag="VLN25_05265"
     CDS             1053..>2613
                     /locus_tag="VLN25_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron-transfer flavoprotein:ubiquinone
                     oxidoreductase"
                     /protein_id="HSQ10009.1"
                     /translation="MAESAESIEREAMEYDVVVVGGGPAGLAAAIRLKQRAAESGRDV
                     SVCVLEKGSEIGAHILSGAVMDPIALNELVPDWKERGAPMTTPVTEDRFYILTEKGAI
                     YNPHWLLPDCFINDGYYIVSLANVVRWLGQQAEALGIEIFPGFAAAEVLYDDAGAVRG
                     VATGNMGVGKDGQPTANFQPGVELIGKYTLFAEGARGHLGMQAIQRFKLLEGRDPQTW
                     GIGIKEVWEIDPARHVPGLVVHSAGWPLDSSTYGGGFVYHFENNQVAIGLVVGLGYEN
                     PYLEPFQEFQRYKTHPTIRHFLDGDPARTKRISYGARAINASGLQSLPKLVFPGGALI
                     GCDAGFLNAPRIKGSHAAIKTGMLAADAAFDALAAGRSRDELTAFPAAFEASWLHDEL
                     HRARNFKPYLDKGLYAGAVLWGIDHIVFRGKAPWTLHRTKADHEKLRPAAEYTPIDYP
                     RPDNRITFDKLSSVYLSNTNHEENQPAHLTLRDASVPVAINLARYAGPESRYCPAGVY
                     EFIQVDGTDRLQ"
BASE COUNT          485 a          870 c          800 g          458 t
ORIGIN      
        1 caggatcggc gcttcgccgg tcggcaacgt cgagaaccca aactgctcga accactgtat
       61 gcgggcttcc tccatcaggc ggaagtacat gacgttgttc aaatgtgcca tggcgtccat
      121 gtccccccat cgcaacggca cctcgaggat gaattccttc attcgacaag ctctatgtca
      181 cctacatccc gtcatcggcc gcgatgatgg ctccgttgat gaactgtgac tcctcggcca
      241 cgagcagcag cagcgtgccg tcgaggtctt ccggctggcc gatgcgacgg cgcggcagca
      301 actggatcag cttgcggccc ccttcggtct cccagtattc gtcgttgatc tcggtgcgga
      361 tgtagccggg gcagatggcg ttgacgttga tgccgtagcg gccccattcg agcgcctgcg
      421 cccgcgtcat ctggatcacc gcggccttgc tcatgcagta caacccgatc tgcggcagta
      481 cccgcagccc ggccacggaa gcgatgttga cgattcggcc acggaacccc gggccgctct
      541 tcgaacgcgc aatcatccgc tgcgccacct cctgcgccac gaagaacgcg cccttcacgt
      601 ttgtggcgaa gacatgatcg aagtcggcca acgtggcatc ggtcagccgc tgcgtcgtgg
      661 acacaccgga gttgttgacc agaatgtcaa tcggtccgac cgcacgctcc gtatgctcga
      721 ctgcctcgcg gatcgaattg agatcggtga catccagctg gacgacatgc gccgcgcccc
      781 cggcagcctc gatttccgcg cgcaggtcct tcagccgttc cagtcgccgt gaggccagca
      841 cggtcagtgc accattcgat gcaagaacac gggcaaaacg ggctcccagc ccgctgctgg
      901 ccccggtcac cagcgcgact ttctccttca gattcgtgcg catgcctgct ctaccccttt
      961 ggctccacct tcgagaagca tcgatcgtag tcgattgcag cctctagccg atctccggac
     1021 aattgcctgc atgtcatccc tgcgaggcat ccatggctga atcggccgag agtatcgaac
     1081 gcgaagcgat ggaatacgac gtggtcgtcg tcggcggcgg gccggccgga ctcgccgccg
     1141 ccatccgcct caagcagcgc gcggcggaga gcgggcgcga tgtcagcgtc tgcgtgctgg
     1201 aaaagggttc ggagatcggc gcgcacatcc tttccggcgc ggtcatggac ccgattgcgc
     1261 tgaacgaact cgtgcccgac tggaaggagc gcggcgcgcc gatgacaacc ccagtgaccg
     1321 aggatcgctt ctacatcctc accgagaagg gcgcgatcta caacccgcac tggctgctgc
     1381 ccgactgctt catcaacgac gggtactaca tcgtcagcct tgccaacgtc gtgcgctggc
     1441 tgggccagca ggccgaggca ctcggaatcg agatctttcc cggattcgcg gccgcggaag
     1501 tcctgtacga cgacgccggt gccgtgcgcg gcgtggccac gggcaatatg ggcgtcggca
     1561 aggacggcca gccgaccgca aacttccagc cgggtgtcga gctcatcggc aagtacaccc
     1621 tgtttgccga gggcgcgcgc ggtcacctgg gcatgcaggc gatccagaga ttcaaactgc
     1681 tcgagggccg cgatccgcag acctggggca tcggcatcaa ggaagtgtgg gagatcgatc
     1741 ccgcgcggca tgtgccgggg ctggtcgtgc acagcgcggg ctggccgctg gactcgagta
     1801 cctacggcgg cggcttcgtc taccacttcg agaacaacca ggtcgctatc ggcctggtgg
     1861 tcggactggg ctacgagaat ccgtacctcg aaccgttcca ggaattccag cgctataaga
     1921 cgcatccgac cattcgccac ttcctagacg gcgatccggc caggaccaaa cgcatcagct
     1981 acggcgcacg tgcgatcaac gcgagcgggc tgcagagcct gcccaagctg gtgttcccgg
     2041 gcggtgcgct gataggctgt gacgcgggct ttctcaacgc gccgcgcatc aagggatcgc
     2101 acgcggctat caagaccggc atgctcgcgg ccgatgccgc attcgatgca ctggccgcgg
     2161 ggcgcagccg tgatgaactg acggccttcc cagccgcgtt cgaagcgagc tggttgcacg
     2221 acgagctgca tcgcgcgcgc aatttcaagc cgtatctgga caaggggctg tatgccggcg
     2281 cggtgctgtg gggcatcgac catatcgtct ttcgcggcaa ggcaccgtgg acgctgcaca
     2341 ggaccaaggc cgatcacgag aagctcaggc ccgccgcgga gtacacgccg atcgactatc
     2401 cgaggccaga caaccgcatc acgttcgaca aactgtcctc ggtgtacctt tcgaacacca
     2461 atcacgagga aaaccagccc gcgcacctca ccctgcgcga cgcctccgtg ccggtcgcta
     2521 tcaacctcgc ccgctatgcc gggcccgagt cgcgctattg cccggccggc gtctacgagt
     2581 tcatccaggt cgacggtacg gatcgactgc aga
//
LOCUS       DATIRT010000225         2640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1015828, whole genome shotgun sequence.
ACCESSION   DATIRT010000225 DATIRT010000000
VERSION     DATIRT010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015828"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /gene="urtC"
                     /locus_tag="VLN25_05270"
     CDS             complement(<1..782)
                     /gene="urtC"
                     /locus_tag="VLN25_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HSQ10010.1"
                     /translation="MTRLFPLRVWTTVFAVTAGLLIVIPVLHLAVPESSPLHVSSFWI
                     TLLGKFMCYAVVALAMDLIWGYTGILSLGQGLFFALGGYAFGMYLMRQIGREGVYQSD
                     LPDFMVFLDWKEFPWYWAGSDSFLWTIVLVVALPGVVAFIFGFFAFRSRIKGVYFSII
                     TQALTFAAMLAFFRNDMGFGGNNGFTDFKRILGFPITAPETRTVLYVLSGIYLLAALL
                     LARWIVSSKLGRVLTAIRDQESRLMFLGYRTLYFKLFIWTLAA"
     gene            complement(779..2392)
                     /gene="urtB"
                     /locus_tag="VLN25_05275"
     CDS             complement(779..2392)
                     /gene="urtB"
                     /locus_tag="VLN25_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HSQ10011.1"
                     /translation="MLLRFLRAFACVLAFLAAPAEAALDPSLVAQLGAEDSDRRVEAI
                     RKLVATEDPRVIGILKALDSDSFALIDNGQRAVIVSGGQLSDAATGQPAGAAPANLDT
                     VVINNRIRGELGAALAALRLGSSDRAERLAAAKELQGSDNEDALPIIERAIAKETDPD
                     IKGMLALAGASLALKSSESELRARAARALAASDDPQVRQLLATLLTKQKDGSYPEADE
                     GVRAAAQASLAEINARLRTTEIGLALFSGLSLGSVLLLAALGLAITYGLMGIINMAHG
                     EFLMIGAYATYVVQGVFRAHFPGAFDWYVVAALPVAFAVTAIIGMALERGVLRWLYGR
                     PLETLLTTWGVSLLLIQASRTIFGAQNVEVENPSWLSGGFVLGGGYVLQFNRIVIVLF
                     ALAVLAAVWLVINRTRMGLYVRGVTQNRAMASCTGVPMNRVDMLTFGLGSGVAGLGGV
                     ALSQIGNVGPDLGQSYIVDSFMVVVLGGVGQLAGTVIAAFGLGSIAKFLEPWSGAVLA
                     KIMVLVLIILFIQKRPQGLFALKGRSAEA"
     gene            complement(2463..>2640)
                     /locus_tag="VLN25_05280"
     CDS             complement(2463..>2640)
                     /locus_tag="VLN25_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding protein"
                     /protein_id="HSQ10012.1"
                     /translation="GFTIKMDETNHHLWKPVMIGEIRPDGQFNVVWKTKGPVRAQPWS
                     PFIPGNEKKLSTPV"
BASE COUNT          519 a          842 c          866 g          413 t
ORIGIN      
        1 gcggccaggg tccagatgaa caacttgaag tacagcgtgc ggtaaccgag gaacatcagt
       61 cgcgattcct ggtcccggat cgcggtgagc acgcgaccga gcttgctcga cacaatccag
      121 cgcgccagca gcagtgcagc gagcagatag attcccgaca gcacgtacag caccgtgcgg
      181 gtttccggcg cggtgatcgg gaatccgagg atgcgcttga agtcggtgaa gccattgtta
      241 ccgccgaagc ccatgtcatt gcggaagaac gccagcatcg ctgcgaacgt cagcgcctgc
      301 gtgatgatcg agaagtagac gcccttgatg cgtgagcgga acgcgaagaa tccgaagatg
      361 aacgcgacca cccccggcag ggcgacgacg aggacgatcg tccagaggaa cgagtcgctg
      421 ccggcccagt accaggggaa ctccttccag tcgaggaaca ccatgaagtc gggcaggtcg
      481 ctctggtaga cgccttcgcg cccgatctgg cgcattaggt acatgccgaa cgcatagcct
      541 ccgagtgcga agaacaggcc ctgcccgagg gacaggatgc cggtgtagcc ccagatcagg
      601 tccatcgcga gcgccacgac ggcgtagcac atgaacttgc ccagcagcgt gatccagaaa
      661 ctcgatacat gcagcggact cgactccggg accgcgaggt gcagcaccgg gatgacgatg
      721 agcagtcctg cggtcaccgc gaaaaccgtc gtccacacac ggagaggaaa gaggcgcgtc
      781 acgcttctgc gctccgtcct ttgagggcaa acagcccctg gggacgcttt tgaatgaaca
      841 gaatgatgag caccagcacc atgattttcg ccagcacggc gcccgaccac ggctcgagga
      901 acttggcgat cgagccgagc ccgaaggccg cgatgacggt acccgcgagt tgcccgacgc
      961 cgccgagcac cacgaccatg aagctgtcga cgatgtagct ctgtccgagg tcgggaccga
     1021 cattgccgat ctgtgagagc gccacgccgc ccaggcccgc gacgccggac ccgagcccga
     1081 aggtcagcat gtccacgcga ttcatcggca cgccggtgca gctggccatc gcgcggttct
     1141 gcgtgacgcc acgaacgtac agtcccatcc gcgttcggtt gattaccagc cagacggcgg
     1201 ccagcacggc gagggcgaaa agaacgatga cgatgcgatt gaactgcagc acgtagccgc
     1261 cgccgagcac gaagccgccc gagagccagc tcgggttctc gacctcgaca ttctgtgcac
     1321 cgaagatcgt gcggctggcc tggatcagca gcaggctcac gccccaagtg gtcagcaatg
     1381 tttcgagcgg ccggccgtac aaccagcgca gcacgccgcg ctcgagcgcc atgccgatga
     1441 tggctgtcac cgcgaacgcg acaggcagcg ccgccacgac ataccagtcg aacgcgccgg
     1501 gaaagtgcgc gcggaacacg ccttgcacga cgtaggtcgc ataggcgccg atcatcagga
     1561 attcaccgtg ggccatgttg atgatgccca tcagcccgta ggtgatcgcc agcccgaggg
     1621 cggccagcag caggaccgag ccgagactca acccggaaaa aagcgccagt ccgatctcgg
     1681 tcgtgcgcag ccgtgcgttg atctcggcga gcgacgcctg tgcggctgcg cgcacgccct
     1741 cgtcggcttc gggatagctg ccgtctttct gcttcgtcag cagggtggcg agcagctgcc
     1801 gcacctgcgg atcgtcgctg gcggcgagcg cgcgcgcagc gcgtgcgcgc agctcggatt
     1861 cgctgctttt cagcgcgagc gaagcgccgg cgagtgcaag catgcccttg atgtcgggat
     1921 cggtttcctt cgcgatggca cgctcgatga tcggcagcgc gtcctcgttg tcggagccct
     1981 gcagttcctt tgccgcggca agacgttcgg cgcggtcgga gctacccagg cgcaaggccg
     2041 ccaaggctgc gccgagttca ccgcggatgc ggttgttgat gacgacggta tcgaggttcg
     2101 ccggcgcggc gccggcaggt tgcccggtgg cggcgtcgct cagttggccg cctgacacga
     2161 tgacggcgcg ctggccattg tcgatcagcg caaacgagtc gctgtcgagc gccttgagga
     2221 tgccgatgac gcgtggatct tcggtcgcga cgagcttgcg gatcgcctcg acgcgcctgt
     2281 cggaatcctc cgcacccagc tgggcgacca gcgaaggatc gagtgcggcc tcggccggcg
     2341 cagcgaggaa cgccagcacg caggcgaatg cgcgcaggaa acggagcagc atcgggatgc
     2401 aaggcaagac gcgcagatca ccgccgttcc aggggaccgg tgacctgcgc gcctgcggtt
     2461 gcctagaccg gcgtactgag cttcttctcg ttacccggga tgaaggggct ccacggttgt
     2521 gcacgcaccg ggcccttcgt cttccatacg acgttgaact ggccgtccgg acggatctca
     2581 ccgatcatca ccggcttcca gaggtggtga ttggtttcat ccatcttgat cgtgaatccg
//
LOCUS       DATIRT010000226         2512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1605516, whole genome shotgun sequence.
ACCESSION   DATIRT010000226 DATIRT010000000
VERSION     DATIRT010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1605516"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VLN25_05285"
     CDS             <1..763
                     /locus_tag="VLN25_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSQ10013.1"
                     /translation="NSFPVNGARQSYRLTRALQQQIEDLSQASRMTELPPVITFQSVL
                     DFTVSTPAILSALYAHLPDNGSEIVLFDVNRTIKFGPLLRASSDNALARLVPTSPQSY
                     RFTAIANVSPDSTATVERSIAPNQSSEQTRPLELPYPVEIFSLSHVAVPFPLNDPLYG
                     LQPDLKGEEQFGVSLGTLSARGERGALVVDADFLFRIASNPFFPYMLERIEQGITASK
                     ATATKKAPPHSVPRLREADAAEAFPDEADAAQSGP"
     gene            964..2109
                     /locus_tag="VLN25_05290"
     CDS             964..2109
                     /locus_tag="VLN25_05290"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSQ10014.1"
                     /translation="MLPLLAAAMVSLSGCATSPPPAPPPEAFLHDELFGPPSVRVSGD
                     GIFALSDAMKRYLRTEIATQTRLHGVPGGLVEAVYNKAQLKLEYDSSFTRNASEAFEA
                     RAGNCLSLVVMTAAFAKELGLQVRYQSAYLEEAWSRSGDLLLRAGHVNVTLGPKLQDR
                     ASPLSRSTTIDFLPGEQLRTLRTREISEQTVIAMYMNNRAVEALMRGKLDDAYAWARA
                     SLRQSPDFLSAHNTLGIIYARRGHYDHAATAYQHVIRNDPVHTRAIANLADVYVKLGR
                     KDEAAALYMTLARLEPEPPMHYFNQGMTAMQRGDFGSARDLFAREADRSGYSSDVTFW
                     LGIAHYRLGDVEQATRLLNQAFGNSKSRGDAESQSAKLSRLKSRGLQ"
     gene            complement(2168..2434)
                     /locus_tag="VLN25_05295"
     CDS             complement(2168..2434)
                     /locus_tag="VLN25_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10015.1"
                     /translation="MQKLLATAIVSAFALIQAPAFAQDKPMEKKAEPAKAEAKKAEPA
                     TAAAPEAKKADPAKAAPDAKKAETAKAAPEAKKEEQKKPKKGGC"
BASE COUNT          432 a          823 c          781 g          476 t
ORIGIN      
        1 caactcgttc ccggtgaatg gcgcgcggca gtcgtaccgg ctgactcgcg cgctgcagca
       61 gcagatcgag gacttgtcac aggcgagccg gatgacggag ctgcctccgg tgatcacctt
      121 tcagtccgtg ctggacttca ccgtgagcac ccccgcgatc ctgtcggcgc tgtatgcaca
      181 tctgcccgac aacggcagcg aaatcgtgct gttcgatgtg aatcggacga tcaagttcgg
      241 gcctttgctg cgggcgtcct ccgacaatgc gctcgcacgg ctggtcccga catcgccgca
      301 gtcgtaccgg ttcaccgcca tcgccaatgt gtcgccggac agcaccgcga ccgtcgaaag
      361 gtcgatcgca ccgaaccaat ccagcgagca gacacggccc ctggagctgc cctacccggt
      421 cgagatcttc tcgctgtcac acgtggcggt gccgtttccg ctgaatgatc cgctgtacgg
      481 cctgcaaccc gatctcaagg gcgaggaaca gttcggagtc agcctgggca cgctgtcggc
      541 gcgcggtgag cggggtgcgc tcgtcgtcga tgcggacttt ctctttcgca tcgcgtccaa
      601 tccattcttt ccgtacatgc tcgagcgcat cgagcagggg atcacggcgt ccaaagcgac
      661 cgcgaccaag aaggcgccgc cacattccgt ccccaggctg cgcgaggccg acgcagcgga
      721 ggcgtttcca gacgaggccg acgcggcgca gtctggcccc tgagtcatca gccgaacggc
      781 gggacacgcg gcacagcgac ctgacgcacg gacgcggggg cgcctgcact ttgcgcggct
      841 caggagtagg atggactggc attccataac gttgacttgc cgttgacttt ccgttgacga
      901 ctcatggcaa ttgccttcac gacccgctgg aaaaccgggg cttgcgccgc ccggagcgca
      961 atgatgctgc cgctgcttgc ggccgccatg gtttcacttt ccggctgcgc gacctcgccg
     1021 ccgcccgcgc ctccccctga ggccttcctg cacgacgagc tcttcggccc gccgtccgtg
     1081 cgcgtcagtg gcgacgggat cttcgcgctg agcgacgcga tgaagcgcta cctgcgaact
     1141 gagatcgcca cgcagacccg attgcatggc gtcccgggcg gactggtcga ggcggtctac
     1201 aacaaggcgc aactgaagct cgagtacgac tcttcgttca cgcgcaacgc ctccgaagcc
     1261 ttcgaggcac gcgccggtaa ctgcctgtcg ctggtcgtga tgaccgccgc ctttgcgaaa
     1321 gagctcggat tgcaggtgcg ctaccagagc gcctacctgg aagaagcgtg gagccgtagc
     1381 ggggatctgc tgctgcgcgc gggtcacgtc aacgtgacgc tcggaccgaa gctgcaggat
     1441 cgcgccagtc ccctgtcgcg ctcaacgacg attgactttc tgccgggaga acagctgcgc
     1501 acgttgcgca cgcgggagat ctcggagcag accgtgatcg cgatgtacat gaacaatcgc
     1561 gcggtcgagg ccctgatgcg tggcaagctc gatgatgcct atgcctgggc tcgcgcgtcc
     1621 ctgcgccaga gtccggactt cctgagcgcc cacaatacgc tgggcatcat ttatgcgcgc
     1681 aggggacatt acgaccacgc ggcgaccgcc taccagcatg tgatcaggaa cgaccctgtg
     1741 cacacgcgtg cgatcgccaa tctcgccgac gtctatgtga agcttggtcg caaggacgag
     1801 gcggcggcgc tgtacatgac actggcgcgc ctggaaccgg aacctccgat gcactacttc
     1861 aaccagggga tgacggccat gcagcgtggt gacttcggct ccgcgcgcga tctgtttgca
     1921 cgcgaggcgg accgcagcgg ctactcgagc gacgtcactt tctggctcgg catcgcgcac
     1981 tatcgacttg gagatgtcga acaggcgacg cgcttgctga accaggcctt cggcaacagc
     2041 aagtctcgcg gtgatgccga atcgcaatcg gccaagctga gccgtctgaa gtcacgcggc
     2101 ctgcagtaac tggccgcaaa tgaaaacgtc tccggaccga gtggtccgga gacgttttgg
     2161 gaactgatta gcagccgccc tttttcggct tcttctgctc ttccttcttg gcctcaggcg
     2221 ccgccttcgc ggtctccgcc ttcttggcat ctggcgccgc cttcgcggga tcggctttct
     2281 tggcctcagg cgccgccgcc gtcgcaggct ccgccttctt ggcctcagcc ttggccggct
     2341 cggccttctt ctccatcggc ttgtcctgcg caaaggcggg tgcctggatc agtgcaaagg
     2401 cggacacgat ggcggtagcg agcagtttct gcatgggttc tatctccctg agtttgttga
     2461 agtcatgcca taaggccaat cgctaacggg tcggcacgac tttcggttga cc
//
LOCUS       DATIRT010000227         2372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1704234, whole genome shotgun sequence.
ACCESSION   DATIRT010000227 DATIRT010000000
VERSION     DATIRT010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1704234"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..443
                     /locus_tag="VLN25_05300"
                     /pseudo
     CDS             270..443
                     /locus_tag="VLN25_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
     gene            729..1706
                     /locus_tag="VLN25_05305"
                     /pseudo
     CDS             729..1706
                     /locus_tag="VLN25_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            1821..>2372
                     /locus_tag="VLN25_05310"
     CDS             1821..>2372
                     /locus_tag="VLN25_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSQ10016.1"
                     /translation="MPNTVPAIVVDKLCKHYGALKAVDDISFSVKSGEVFAFLGPNGA
                     GKSTTAEVIETIRRPTSGTVTVLGMDVTKRKAEVVRQIGVLPQNFSSFDRITVHETLQ
                     YYASVFGCRADIDALMGIANLRDKSAARFNTLSGGLKQRLGIAVALVCDPEIVFLDEP
                     TTGLDPHARRQVWDVLKGLKDRGK"
BASE COUNT          423 a          843 c          714 g          392 t
ORIGIN      
        1 gtgcgcagcg tctcgtcctc gtacacgagg tggtccgtga cgggagtgtc gatcttgcgc
       61 cggtcatcga cgcgatccgc gccgcagttc tcgaggtgca cgggctggcg ctcgatgccg
      121 tcgttctgat ccgatgcggc accatcgcca agacctccag cgggaaggtc cagcggcacg
      181 cggtccgcgc ggcatttcgc agcgacggac tgaaagcgct tgcccagtac cgcacaccgt
      241 cccggctcga atcctcggca cctggcccga tgcgcacgcc tccggcgcac tcgaccgcac
      301 tcgccgccgt atgccagcac gcgctcgcaa tatcgggcac tggactcgcc gacgtcacgc
      361 ccgaaacacc catcgaggca ctcggcctcg actccttgca gcgcgtcgag ctgattgccg
      421 cacttgacca gagcttcgga cgccacctcc cggacgccgt ttacagccag gcgacgaccc
      481 tcggcgaact cgcggcagcc gtgcagaagc accttatcga tcacccgcat tccgacgtgt
      541 ccgcgaacga cgtccccgcg gagaactaca acgtagcgct gttccccgag tacctggagc
      601 tcgaacgcca caagcacatg ctgctggcag taaccgaaac gaacccttac tttcgtgtgg
      661 acctgggcga cgacacatgg cggcagggta cccgcggcct cactcacgtc aacggcgcgg
      721 ctctgacgaa cttctgcgcc tacgactaca tcgggatggc gcgcgacccg acagtcactg
      781 tagccaccaa ggacgcgatt gatagctatg gcacgggcgc gggagccagc cgcctcgtct
      841 ccggcgaaaa gcagattcat cgcgacctcg agcacgcact cgccggattt ctcggcacac
      901 ctgcgacgat cgttttcgtc tccgggcacg cgaccaacgt cacgacgatc ggccacctga
      961 tgggcccggg cgacctgatc gtccacgaca tcctggccca caactcgatt ctccagggtg
     1021 cgcggctctc gggcgccacg tgccgcggat tcgcccacaa cgactggcgc gcgctcgacg
     1081 tgctccttgc aggtatccgc cacagctacc gccgcgtgct catcgcgatc gagggcgtct
     1141 acagcatgga tggcgactac cccgacctgg cgcgcttcgt cgacatcaag gaaaggcatc
     1201 acgcgctgct ctatgtcgac gaggcgcact cgctcggaac gatgggcagg accggccgcg
     1261 gcctcggcga acacgccggc gtcgccccct ccagcgtcga cgtgtggatg ggcacgctgt
     1321 ccaaatcgct tgcaagttgc ggcggctaca tcgccggatc aggcgaactc atcgagtacc
     1381 tcaagtacac ggcgcccggg ttcgtctaca gcgtcggaat ctcgccgccg aacgcggcca
     1441 cggcgctcgc cgccctggaa gtcctgcagc gcgagccgca acgcgtcgcg cggctgcggc
     1501 agttgtcgag tctcttcctc cgactcgccc gtgaacgtgg gctcaatacg ggggtcgccg
     1561 cgggaacacc ggtgatcccg atcatcgtcg gcagctccgt ccgctgcctt cggctcaccc
     1621 gcgctctgtt ccgtcgcggc ctcaacgtcc agccgatcat tcatccggcc gttcccgaac
     1681 aggccacgcg gctgcgggta ttcatcacga tcaatcacac cgagtcgctc atccggggcg
     1741 ccgtcgacgc gatcgcagag gaactcgcga agctctagca cggccgtcgc gcccgtgcag
     1801 cgcccgcgaa gcgcttcatc atgccgaaca ccgtacccgc aatcgtggtc gacaagctct
     1861 gcaagcacta cggcgcattg aaggccgtcg acgacatctc gttcagcgtc aagtcgggcg
     1921 aagtgttcgc gtttctcggc cccaatggcg cggggaagag cacgaccgcc gaagtgatcg
     1981 agaccatccg caggccgaca tccggtacgg tcacggtgct cggtatggac gtcacgaagc
     2041 gcaaggccga ggtggtccgc cagatcggcg tgctgccgca gaacttcagc tcgttcgatc
     2101 gcatcacggt gcacgagact ttgcagtact acgccagcgt gtttggctgc cgcgccgaca
     2161 ttgacgccct gatgggcatc gccaatctgc gcgacaagtc tgcggcccgg ttcaacaccc
     2221 tctctggtgg tctcaagcaa cgcctcggga tcgccgtcgc gctggtctgt gacccggaga
     2281 tcgtctttct cgatgaaccc acgaccggac tcgaccccca cgcccggcgt caggtctggg
     2341 acgtgctcaa gggactgaag gaccgtggga ag
//
LOCUS       DATIRT010000228         3179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1015988, whole genome shotgun sequence.
ACCESSION   DATIRT010000228 DATIRT010000000
VERSION     DATIRT010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015988"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1909
                     /locus_tag="VLN25_05315"
     CDS             <1..1909
                     /locus_tag="VLN25_05315"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HSQ10017.1"
                     /translation="TALVFFQSTLLAGYAYADLTQRLGVRRQTLLHVGLLLLSLLTLP
                     ILASPEWKPQGDEAPIARILLLLVATIGLPYFLLSTTTPLLQAWYWRRVRAAVPYRLF
                     ALSNFASLLALLGFPVIFEPWLSLPQLGWAWSVMYGSFVATCAATAWISLRATADDDS
                     HELPPSATAGTAPAIAPTLSMQLRWLLLAAMGSVMLLAVTNHVTQNVASVPFLWVLPL
                     SLYLLTFVLAFDHPRWYRQPVYVLAMALAVPAMAWLIPSLDLKLAASVFFAGLFIACM
                     FCHGELARMRPDPRYLTRFYLMISLGGALGAVLVAIVAPLVLRGYFEVNIALVLLALL
                     VLLQLSGWKRLIGVAVVGATAYFALTGAYEYSRGLRVMERDFYGVVRTRDRQDPVPYR
                     AMLHGGIIHGGQLLGDNFRNTPSDYFGPGSGYGRLFAALNEIEPAPRRVGIVGLGAGV
                     VASYGRPGDIFTFYEISPRVVDVAHSEFTFLRDTAAKTEVVLGDGRLSLEREPPRRYD
                     VLGIDAFAGDSIPMHLVTREAMALYVRHLAPDGVIVFQATNRYIDLLPVVKRLANEVG
                     MEAVLVSDVPAGSDTGPDYWLSSTDQILVTRNRRLLESGALRYVASPIADRPDLATFT
                     DAHHNLFRILK"
     gene            complement(1935..2852)
                     /locus_tag="VLN25_05320"
     CDS             complement(1935..2852)
                     /locus_tag="VLN25_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HSQ10018.1"
                     /translation="MQLPPLKGIIAFEVVARLGSINKAADELNVTASAVSHQISNLEG
                     FVGRKLFERTSRGLVLTPVGVRYQNDVTGALALIASAAHNARNAEGVEVLRIHVSPSF
                     ASLWLMPRLPAFLAEHPDLRIQLSASHTHSDFSRAEVDLDIRYGAIKWGELHVETIFD
                     EEMLPLASPTLLGRAVMLSPEQLLAQPLIFSDVNVVQWPRWFAAHGVPLSPATYALRF
                     DRAYLVIEAAIQGLGVALESARLSEKALSNGDLVPVFADRKGIQVHAHHLVYPESHGK
                     WSKVVRFVTWLRREAAAQPPAPKTRSKGR"
     gene            2956..>3179
                     /locus_tag="VLN25_05325"
     CDS             2956..>3179
                     /locus_tag="VLN25_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSQ10019.1"
                     /translation="MLLKDKVAIITGAASARGIGKATARLFAEHGARVAILDLTAEAA
                     EQAAADIGSGHIGLACNVTSKPDCERAAAAV"
BASE COUNT          494 a         1030 c         1036 g          619 t
ORIGIN      
        1 cacggcgctg gtgttcttcc agtcgacact gctggccggt tacgcctacg cggacctgac
       61 acaacggctg ggcgtgcggc gccagacgct gctgcacgtc ggattgctgc tgctgtcgct
      121 gctgacgctg ccgatcctgg cgtcaccgga gtggaaaccg cagggtgacg aggcgccgat
      181 cgcccgcatt ctcctgttgc tcgttgcgac catcggtctg ccgtacttcc tgctgtcgac
      241 gacgacaccg ctgctgcagg cgtggtactg gcggcgcgtc cgtgccgcgg ttccgtaccg
      301 gttgttcgcg ctgtccaact tcgcgtcgct gctggcactg ctcggcttcc cggtcatttt
      361 cgagccgtgg ctttccttgc cacagcttgg ctgggcgtgg tcggtgatgt acggctcctt
      421 cgtggcgacc tgtgctgcga cggcctggat atcgttgcgc gccaccgcgg acgatgactc
      481 gcacgagctg ccgccatccg cgacggctgg tacggcacct gccatcgctc cgacgctgtc
      541 gatgcaattg cgctggctgc tgctggccgc catgggatcg gtgatgctgc tggccgtcac
      601 caatcacgtg acgcagaacg tcgccagtgt tccgttcctg tgggtgctac cgctgtcgct
      661 gtacctgctc acgttcgtgc tcgctttcga tcatccgcgc tggtatcggc agccggtcta
      721 cgttttggcg atggcgctcg cggtgccggc gatggcatgg ctgatcccgt cgcttgactt
      781 gaagctcgcg gcgtcggtct tctttgcggg gctttttatc gcttgcatgt tctgccatgg
      841 cgaactggcg cgcatgcgcc cggatccgcg ttacctcacg cgcttttacc tgatgatttc
      901 cctcggcggc gcactcggcg cggtgctggt cgccatcgtg gcgcccctgg tgctacgcgg
      961 atacttcgaa gtgaacatcg cgctggtgct gctggcgttg ctggtgctgt tgcagctcag
     1021 cggctggaag cgcctgatcg gggtcgctgt cgtgggcgcg acagcgtact ttgcgctgac
     1081 cggagcctat gagtactcga ggggcctgcg ggtgatggag cgcgatttct acggtgtcgt
     1141 tcgcacgcgc gaccggcaag accccgtgcc gtaccgggcg atgctgcatg ggggcatcat
     1201 ccacggggga caactgctcg gagacaactt ccgcaacacg cccagcgact actttggacc
     1261 gggttccggc tatggacgcc tgtttgccgc gctcaacgaa atcgagccgg cgccacgccg
     1321 ggtcggcatc gtcgggctcg gtgcaggcgt ggtggcttcc tacggacggc cgggcgacat
     1381 cttcacgttc tacgagatct cgccgcgggt cgtcgatgtc gcacactcgg agttcacctt
     1441 cctgcgcgac accgccgcca agaccgaggt cgtgctcggc gatggccgcc tgtcactcga
     1501 gcgcgaaccc ccgcggcggt atgacgtgct cggcatcgac gccttcgccg gcgactcgat
     1561 accgatgcac ctggtgacgc gcgaggcgat ggcgctgtac gtccgccatc tcgctcccga
     1621 cggcgtcatc gtattccagg cgaccaaccg ctacatcgac ctgctgcccg tggtgaagcg
     1681 cctggcgaat gaagtcggca tggaagcggt gctcgtgtcc gacgttccgg cagggtcgga
     1741 caccggaccc gactactggc tctcgtcgac cgaccagatc ctggtgaccc gcaatcgccg
     1801 gctgctcgag tcaggtgcat tgcgctacgt cgcttcaccg attgcggacc gacccgatct
     1861 ggcaacgttc acggacgcac accacaacct gttccgcatc ctcaagtgag ccgagggcaa
     1921 ccttcgacgg ggattcagcg ccccttcgat ctggtctttg gtgcgggtgg ctgagcggcc
     1981 gcttcgcggc gcaaccaggt tacgaagcgc acgaccttgc tccacttgcc gtgcgactcg
     2041 gggtagacga ggtggtgtgc gtggacctgg attcctttcc ggtcggcaaa gaccggtaca
     2101 agatcgccat tgctcagggc tttctctgac aggcgggcgc tctccagtgc cacgcccagt
     2161 ccctgtatgg ctgcctcgat caccaggtac gcgcgatcga atcgcagtgc atacgtggca
     2221 ggactgagcg gaacgccgtg ggccgcgaac catcgcggcc attgaacgac gttgacgtcg
     2281 gaaaagatca gcggctgtgc gagcaactgc tcggggctca gcatgacggc gcgccccagc
     2341 agggtcggac tggccagcgg cagcatctcc tcgtcgaaga tcgtctcgac gtgcagttca
     2401 ccccatttga tcgcgccgta gcggatgtcg aggtcgactt ccgcgcgcga gaaatcggaa
     2461 tgcgtgtgcg acgccgaaag ctggatgcgc aggtccggat gctcggccag gaacgcgggt
     2521 aaccgcggca tcagccagag actggcaaag ctcggtgaaa cgtggatgcg caggacctcg
     2581 acgccctctg cgttgcgcgc gttgtgtgcc gcgctcgcga tcagcgccag cgcgccggtc
     2641 acgtcattct ggtagcgcac tccgacgggc gtgagcacca gcccgcgcga ggtccgctcg
     2701 aagagtttgc gtccgacaaa gccttccagg ttggagatct gatggctgac cgccgaagcc
     2761 gtgacgttga gttcatcggc cgccttgtta atgctgccga ggcgggcgac gacttcgaac
     2821 gcaatgattc cctttagcgg aggcagttgc atcgcagcat gaaattaatt cagtgcggcc
     2881 tgagggcggc tagcttgctg gcattcgagc gcaaacgcaa catgcgtcca cattcaaacc
     2941 gcgctggaga ccgccatgct gctgaaggac aaagtcgcaa tcatcaccgg ggccgcgtcc
     3001 gcgcgcggca tcggcaaagc caccgcgcgg ctcttcgccg agcacggagc gcgtgtcgcg
     3061 atactcgacc tgaccgccga agcggccgaa caggccgccg cggacatcgg ttccggccac
     3121 atcggcctgg cgtgcaatgt caccagcaag ccggactgcg agcgcgccgc cgccgcggt
//
LOCUS       DATIRT010000229         2252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_130431, whole genome shotgun sequence.
ACCESSION   DATIRT010000229 DATIRT010000000
VERSION     DATIRT010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130431"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VLN25_05330"
     CDS             complement(<1..374)
                     /locus_tag="VLN25_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSQ10020.1"
                     /translation="MAQNRLMLDYVLDHEEKLRNSVYMTQPIGNNQVVDTTWGQTVDQ
                     ARRMAAYLQSKGFPRGARVAILSKNCSHFIMAELAIWMAGYTTVAIFPTETAETINYV
                     LTHSEASLLFVGKLDTWPHQQPG"
     gene            528..1343
                     /locus_tag="VLN25_05335"
     CDS             528..1343
                     /locus_tag="VLN25_05335"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HSQ10021.1"
                     /translation="MRQYEDFMRHVFEHGVVKADRTGTGTRSWFGYQLRFDLGEGFPL
                     VTTKKLHLRSIIHELLWFLQGSSNVAYLRDNKVTIWDEWARPDGELGPVYGVQWRSWP
                     TPDGGSIDQIAQVVREIRANPDSRRLIVSAWNVSDIPKMALPPCHLLFQFYVAPGESP
                     SAPGKLSCQLYQRSCDIFLGVPFNIASYALLTHMVAQQCDLDVGDFVWTGGDCHIYSN
                     HFEQVREQLAREPRPYPRLAIRRKPPTLFEYAFDDFEIVDYDPHPHIKAPVAV"
     gene            1343..>2252
                     /locus_tag="VLN25_05340"
     CDS             1343..>2252
                     /locus_tag="VLN25_05340"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSQ10022.1"
                     /translation="MAAASNAELLRPQPAAHALALPARCYVDTAVEQAERNTVFARGW
                     QLVAHAGQVERPGDHVVAEIGGVPLLIVRGEPEASAPLGRLRALHNVCRHRAGPLALC
                     DGRGAKSLVCRYHGWSYALDGQLRGAPEMHDALGFDLGAIRLPEARVAEWQGLVFAAL
                     DTDGDRAVDFAETVAGIDDRLTQPLTRLVFDRRVSYEIRCNWKVYVDNYLEGYHLPRV
                     HPGLNRLLDYRSYRTELAPWHSLQWSPLDTAQGPYRADDEAAVAQYWWIWPNTMLNVL
                     PGRLQTNRVVPLEAQRCRVDFDYYYPA"
BASE COUNT          376 a          740 c          722 g          414 t
ORIGIN      
        1 ccgggctgct ggtgcggcca cgtgtcgagc ttgccgacga aaagcaggct ggcctcgctg
       61 tgcgtcagga cgtaattgat cgtctcggct gtttcggtcg gaaagatggc cacggtcgta
      121 tacccggcca tccagatcgc cagctccgcc atgatgaagt gcgagcagtt cttcgagaga
      181 atcgcgacgc gcgcaccacg ggggaaaccc ttgctctgca ggtatgcggc catgcgccgc
      241 gcctggtcga ccgtctggcc ccaagtcgtg tccacgacct ggttgttgcc gatcggctgc
      301 gtcatgtaga cgctgttgcg aagtttttcc tcgtggtcga ggacgtaatc cagcatcagc
      361 cggttctgcg ccatgttgtc tcctccgtcg tctgtctcgc gcggtggcgc agcctactgc
      421 aaccgaggcg gtccggccat agaattgcag ccgattcgta ttgcgccact agtgcggcgc
      481 ggcgtcgacg caggccgaga gtcgcaccgg cccgatagaa tggcgcgatg cgtcaatatg
      541 aagacttcat gcgccatgtg ttcgagcatg gcgtcgtcaa ggccgaccgc accggcaccg
      601 gcacgcgctc gtggttcggc tatcagctgc gcttcgacct cggcgaaggc ttcccgctcg
      661 tcaccaccaa gaagctgcat ctgcgctcga tcatccacga actgctgtgg ttcctgcagg
      721 gttcgagcaa cgtcgcctac ctgcgcgata acaaggtcac gatctgggac gaatgggcgc
      781 gacccgacgg cgaactcggc ccggtgtacg gggtgcagtg gcgctcgtgg ccgacgcccg
      841 atggcggctc gatcgaccag atcgcgcagg tggtccgtga gatcagggcg aacccggatt
      901 cacggcggct gatcgtctcg gcctggaatg tcagtgatat tccgaagatg gcgctgccgc
      961 catgccacct gctgttccag ttctatgtcg ctcccggtga gtcgccttcg gctccgggaa
     1021 agctgtcctg ccagctctat cagcgcagct gcgacatctt cctcggcgtg cccttcaaca
     1081 tcgccagcta cgcgctgctg acgcacatgg tggcgcagca gtgcgatctg gacgtcggtg
     1141 acttcgtctg gactggcggc gactgccaca tctacagcaa ccacttcgag caggtgcgcg
     1201 agcagctcgc acgcgagcct cggccttacc cgcgcctggc catcagacgc aagcctccca
     1261 cgctcttcga gtacgcgttc gacgacttcg agatcgtcga ctacgacccg catcctcaca
     1321 tcaaggcgcc ggtggccgtg tgatggcggc ggcgtccaac gcggaactgc tgcggccaca
     1381 gcccgccgca cacgccctgg cgctgccagc acgctgctac gtggacaccg cagtcgagca
     1441 agccgaacgc aacacggtct ttgcccgtgg ctggcaactc gtggcgcatg cggggcaggt
     1501 cgaacgtccc ggcgatcatg tcgtagcgga aatcggcggt gtgccgctgc tgatcgtccg
     1561 cggcgagccg gaggcatcgg cgccgctcgg ccgactacgc gcgctgcata acgtctgccg
     1621 ccatcgcgcc ggccctttgg ccctctgtga cggccggggt gcgaagtccc tggtgtgtcg
     1681 ctaccacggc tggagctatg cgctcgacgg gcaactgcgc ggtgcgccgg agatgcacga
     1741 tgccctcggc tttgacctcg gcgcgatccg gctgcccgaa gcacgcgtgg ccgaatggca
     1801 ggggctcgtg ttcgcggcgc tggataccga tggcgacagg gcagtcgact ttgccgaaac
     1861 cgtcgcaggc atcgacgacc ggctgacgca gccgctgacc agacttgtgt tcgatcgacg
     1921 cgtcagctac gaaatccgct gtaactggaa ggtgtacgtc gataactacc tcgagggcta
     1981 ccacctgccg cgtgtgcacc cgggcctgaa ccggctgctc gattaccgca gctatcgcac
     2041 cgaactcgca ccttggcatt cgctgcagtg gagtccgctg gacacggcac agggtccgta
     2101 tcgcgccgac gacgaagcgg ccgtggcgca gtactggtgg atctggccga acacaatgct
     2161 gaacgtgctg cccggtcggc tgcagacgaa tcgtgtcgta ccgctggagg cgcagcgctg
     2221 ccgcgtcgac ttcgactact actacccggc cg
//
LOCUS       DATIRT010000230         4635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1901064, whole genome shotgun sequence.
ACCESSION   DATIRT010000230 DATIRT010000000
VERSION     DATIRT010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1901064"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VLN25_05345"
     CDS             <1..322
                     /locus_tag="VLN25_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10023.1"
                     /translation="DHSDPARQAEYLASLAREARLLEFALDGDGDAARRLAADLESAL
                     ENAMDAESPAELTQACTAIETALSKVLAGGMRLSAQMTDMEVDGVLGTARMPVLTAVL
                     SAAA"
     gene            complement(331..1506)
                     /locus_tag="VLN25_05350"
     CDS             complement(331..1506)
                     /locus_tag="VLN25_05350"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HSQ10024.1"
                     /translation="MATTGERRLAGDEGWAIALRAARHTMVKQEPGEHWRQRRWRRGA
                     VVLAIVVLMHVLAITGVRRELALDRPDKPTQTTMSIALLKPPAPQAAPAPPPPRPRPP
                     PTPRTPSVVTSASPATALAPDEPPPAPEPAPPPAPAPVEAPPPPVTEPAAEPSRLPPG
                     VKEMPTKGRIAYRTTYSRMRGIDVMTYVDWTIDVEQARYELWLRTVDPPGLLDLRSTG
                     ELRPFGIAPTQYLERIEIANRELSVDFDWTRHIVRFAGRGAGEPAPFEDGTQDPLSLQ
                     FHLPLLAQTYPWRFTPGSIVSFQVARRKVESYTFVADGFEPVTIQGKELQALKVLRPK
                     GPNSSRAVELWMAPDLDWLPIRLRFVDTNDEVWDSVLAHLPGTEPPAAPIQQEAVKP"
     gene            1582..2571
                     /locus_tag="VLN25_05355"
     CDS             1582..2571
                     /locus_tag="VLN25_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016724204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HSQ10025.1"
                     /translation="MKLATLKDGSRDGQLVVVSRDLALAHHAAGIVGRLQGALDDWSF
                     FAPQLDDLYQTLNEGRARHAFAFDARVAMAPLPRAYQWVDGSAYVNHVELVRKARGAE
                     MPASFWTDPLMYQGGSDDLLGPSDPIICPSEEFGIDFEAEVAVITDDVPMGTKPRDAV
                     EHIRLLALVNDVSLRNLIPAELAKGFGFFQSKPATAFSPVAVTPDELGDAWHDAKVHR
                     PLVVHWNEEKVGEPNTGDDMTFDFGQLIAHVAKTRNLRAGSIIGSGTVSNLDRARGYC
                     CIAEKRSLEMIESGAATTPFMKYGDRVRIEMFDGRGQTLFGAIDQRVAGPARR"
     gene            2613..3245
                     /locus_tag="VLN25_05360"
     CDS             2613..3245
                     /locus_tag="VLN25_05360"
                     /inference="COORDINATES: protein motif:HMM:NF043076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhaM family polyhydroxyalkanoate granule
                     multifunctional regulatory protein"
                     /protein_id="HSQ10026.1"
                     /translation="MPDKSNTANPFSGMGDPFEMMQKMWGMAGMPTPGNLASLVRAPQ
                     QMPSMLAPTIDIGELDKRIADLRAVEQWLELNASMLRTTIQTLEVQRATIATLKSISG
                     AMMAPLMGPPPPQRSPFPPIPPEFSSPAAAATMVPPPESAAEAKPARPRRKVASHTPS
                     LSDDALNPAAWWHTLQDQFSKIAAAASETAQAEAKTPARKAKRARRKAKR"
     gene            complement(3286..4068)
                     /locus_tag="VLN25_05365"
     CDS             complement(3286..4068)
                     /locus_tag="VLN25_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSQ10027.1"
                     /translation="MKLANRVAVITGGAQGIGAACARHFAEQGARVVLGDVNEVGAAA
                     LVKDIAAQGGTATFRRTDVGLKADCEALVEHAVAHYGALDILVNNAGIVHAADFLDLA
                     EDDFDRVLRINLKGAFLCAQAAARRMVAQPVRTDGQRGVILNISSVNAVLAIANQVPY
                     TVSKGGLNQLTKVMALGLAPQGIRVMGIGPGSIATELLKTAVLTNEAARQRILSRTPL
                     GRLGEPDEVARIAVFLASDDASYLTGTTLYPDGGRLALNYVM"
     gene            complement(4092..>4635)
                     /locus_tag="VLN25_05370"
     CDS             complement(4092..>4635)
                     /locus_tag="VLN25_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HSQ10028.1"
                     /translation="GYGHRRALPRWYWSGDTHIACMREVVGQTLRYGYAHHIQRLMVT
                     GQFALLAEVEPRQVCDWYLAMYVDAVEWVELPNTAAMALHAAGGRMTSKPYVASGAYV
                     RRQSNYCGECRYRPELRTGDRACPMTTLYWNFLDRHEPELRRNPRTGLMAASIVRLSA
                     QERGAIREQAARMLDNLDSL"
BASE COUNT          765 a         1565 c         1533 g          772 t
ORIGIN      
        1 ggatcactcc gaccccgccc gtcaggccga ataccttgcc tcactggcgc gcgaagcgcg
       61 cctgcttgaa ttcgcgctcg acggcgatgg cgatgcggcg cgtcggctgg cggccgacct
      121 cgaatcggca ctggagaacg ccatggacgc cgaatccccg gccgaactga cacaggcgtg
      181 caccgcgatc gaaacggcgc tcagtaaggt cctggcaggt ggcatgcgcc tgtccgcgca
      241 gatgacggac atggaggtcg acggtgttct gggcacggcg cgcatgccgg tgctgaccgc
      301 cgttctgtct gcggcggcct agcgccactg ttacggtttg accgcctcct gctggatcgg
      361 cgcggctggc ggctcggtgc ccggcaggtg cgcgagcacg ctgtcccaca cctcgtcatt
      421 ggtgtcgaca aagcgcaggc gtatcggcaa ccagtcgaga tccggcgcca tccacagctc
      481 cactgcccgg ctggagttcg gtcccttcgg tcgcaggacc ttgagcgcct gcaactcctt
      541 gccctggatc gtgaccggtt cgaagccgtc ggccacgaac gtatagcttt ccactttgcg
      601 gcgcgcgacc tggaagctga ctatcgatcc cggagtgaag cgccagggat aggtctgcgc
      661 gagcagtggc aggtgaaact gcagcgacaa cgggtcctgt gttccgtcct cgaacggcgc
      721 cggttcgccc gcgccgcggc cggcgaatcg aacgatgtgg cgggtccagt cgaagtcgac
      781 cgacaactcg cggtttgcga tttcgatgcg ctccaggtac tgggtcggcg caatgccgaa
      841 cggcctgagc tcgccggtgg atcgcagatc gagcaacccc gggggatcga cggtgcgcag
      901 ccagagttcg tagcgcgcct gctcgacatc gatcgtccag tcgacatagg tcatcacgtc
      961 gatgccgcgc atgcgcgagt acgtggtgcg gtaggcgatg cgtcccttgg tcggcatttc
     1021 cttgacgccg ggaggcagcc ttgacggttc tgccgcaggc tcggtcacgg ggggcggcgg
     1081 tgcctcgacc ggcgccggcg caggcggcgg tgcgggctcg ggagccggtg gaggttcatc
     1141 aggcgcaagc gcagttgcgg gcgacgcgga agtcacgaca ctcggcgtgc gcggcgtggg
     1201 tggcggcctg ggccgcggtg gcgggggcgc cggtgctgcc tgtggcgccg gcggcttgag
     1261 caatgcgatc gacatcgtgg tttgtgtcgg cttgtccggc ctgtccagcg cgagttcgcg
     1321 ccgcacgccg gtgatcgcca gcacatgcat cagcactacg atcgcgagaa cgaccgcacc
     1381 acgccgccag cggcgttgac gccagtgctc tcccggttcc tgcttcacca tcgtgtgccg
     1441 tgccgcgcgc agcgcgatcg cccaaccctc gtcgcccgcc agtcgtcgct cacccgtcgt
     1501 tgccaccgat tcatccgccg accgcgagcc ccaccgctaa gataccggct tcctcgcttc
     1561 ctcaattccc gatctcatcc gatgaagctc gcgaccttga aggatggttc gcgcgacggg
     1621 caactcgtcg tcgtctcgcg cgacctggcg ctggctcacc acgcggccgg tatcgtcggc
     1681 cggctgcaag gcgcgcttga tgactggtcg ttcttcgcgc cgcagctaga cgatctgtac
     1741 cagacgctca acgaaggacg cgcccggcac gcgttcgcat tcgatgcgcg cgttgcgatg
     1801 gccccgctgc cgcgcgcgta tcagtgggtt gacggctccg cgtatgtcaa ccacgtcgaa
     1861 ctcgtgcgca aggcacgcgg cgccgagatg ccggcttcgt tctggaccga tccgctgatg
     1921 taccagggag gctccgacga cctgctcggg ccgagcgatc cgatcatctg cccgagcgag
     1981 gagttcggca tcgacttcga agccgaggtc gccgtcatca ccgacgacgt tcccatgggc
     2041 acgaagccgc gcgacgcggt ggaacacatc cgcctgctgg cgctcgtgaa cgacgtcagc
     2101 ctgcgcaacc tgatcccggc cgagctcgcg aagggcttcg gctttttcca gagcaagccg
     2161 gcgaccgcgt tttctcccgt ggcggtgacg ccggacgaac tcggtgacgc gtggcacgac
     2221 gcaaaggtcc atcggccgct ggtcgtgcac tggaacgagg aaaaggtcgg agagccaaac
     2281 accggggacg acatgacctt cgactttggg cagctgatcg cccacgtggc aaagacccgc
     2341 aacctgcgcg cgggctcgat tatcggttcc ggaaccgtgt cgaatctcga ccgcgctcgc
     2401 ggttactgct gcatcgctga gaagcgcagc ctcgagatga tcgaatcggg cgccgcgacc
     2461 acgccgttca tgaagtacgg cgaccgggtg cgcatcgaga tgttcgatgg gcgtggccag
     2521 accctcttcg gcgcgatcga ccagcgggtt gccgggccgg cgcgccgcta gcacactgtt
     2581 ccgacttcgc gcccgcatcg agaggagcca ccatgccaga caaatcaaac actgcgaacc
     2641 cgttttcggg gatgggcgat cccttcgaga tgatgcagaa gatgtggggc atggcaggca
     2701 tgccgacgcc cggcaacctc gcgtcactcg tgcgcgcgcc gcagcagatg ccgagcatgc
     2761 tcgcgccgac catcgatatc ggcgagctcg acaagcgcat cgccgacctg cgggcggtcg
     2821 agcagtggct cgaactgaat gccagcatgc tgcgcacgac cattcagaca cttgaagtgc
     2881 agcgcgcgac gatcgccacg ctcaagagca tcagcggcgc gatgatggcg ccattgatgg
     2941 gtccgccgcc gccgcaaagg agtccattcc cgccgattcc tccggaattc agctcccccg
     3001 cagccgctgc gacgatggtc ccgcctccag aatcggcggc cgaggcgaaa ccggctcgcc
     3061 cacgacgcaa ggtagcctcg cataccccgt cgctgtcgga cgatgcactg aatcccgcgg
     3121 cgtggtggca cacgctgcag gaccagttct cgaagatcgc ggcggcggcg tctgaaacgg
     3181 cgcaggctga agccaagaca cccgcgcgca aggcgaagcg cgcgcgccgc aaagcgaaac
     3241 ggtagccggt cctgatatca gcggaccgcg cgcgcgtccg gcgacctaca tcacgtagtt
     3301 gagcgccagg cggccgccat cagggtagag cgtcgttccg gtgaggtacg acgcatcgtc
     3361 gctggcgagg aagaccgcaa tcctggcgac ttcatcgggt tcaccgaggc ggcccagcgg
     3421 cgtgcgcgac aggatgcgtt gccgagccgc ttcgttggtc agcacggcgg tcttcagcag
     3481 ttcggtggcg atcgaaccgg gaccaatgcc catgacgcgg atgccttgcg gcgccagccc
     3541 gagcgccatc accttggtca actggttgag cccgcctttg ctgaccgtgt agggcacctg
     3601 gttggcgatc gccagcaccg cattgacact ggaaatgttg aggatcaccc cgcgctgacc
     3661 gtcggtgcgc acgggttgcg ccaccatgcg ccgcgccgcg gcctgagcgc acaggaacgc
     3721 gcccttgagg ttgatgcgca gcacgcggtc gaagtcgtct tccgccaggt cgaggaaatc
     3781 ggcggcgtgc acgatgccgg cgttgttgac gaggatgtcg agcgcgccgt agtgcgcgac
     3841 cgcgtgctcg accagcgcct cacagtcggc cttcaggccg acgtcggtgc ggcggaaggt
     3901 cgcggttcct ccctgcgcgg cgatgtcctt gaccagtgcg gccgcgccga cttcgttgac
     3961 atcgcccagc acgaccctgg caccctgctc tgcaaagtgg cgggcacagg ctgcaccgat
     4021 tccttgcgcg ccccccgtga tcacggccac ccggttggct agtttcattg ctcgtccctc
     4081 gctcgcggag ttcacagcga gtcgagattg tccagcatcc gcgccgcctg ttcacggatg
     4141 gcgccgcgct cctgcgctga cagccgcacg atcgaggctg ccatcagccc ggtacgcgga
     4201 ttacgccgca gttcgggctc gtggcgatcg aggaagttcc agtacagagt cgtcatcgga
     4261 caggcgcgat cacccgtgcg cagttcgggg cgatagcggc attcgccgca gtagttgctc
     4321 tgccgccgca cataggcacc actggcgaca tacggcttgc tcgtcatgcg gccgccggca
     4381 gcgtgcagcg ccattgcggc cgtattcggc aactcgaccc actcgaccgc gtccacgtac
     4441 atcgccaggt accagtcgca cacctggcgg ggctcgacct ccgccagcag cgcgaactgc
     4501 ccggtcacca tcagccgctg gatgtggtgg gcatagccgt acctcagcgt ctggccgacc
     4561 acttcgcgca tgcacgctat gtgcgtgtcg ccgctccagt accagcgcgg cagtgctcga
     4621 cgatgaccgt agccg
//
LOCUS       DATIRT010000231         1843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1312783, whole genome shotgun sequence.
ACCESSION   DATIRT010000231 DATIRT010000000
VERSION     DATIRT010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312783"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="VLN25_05375"
     CDS             complement(<1..695)
                     /locus_tag="VLN25_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HSQ10029.1"
                     /translation="MSTVATKPRLKTSAMIASIAGEGQRTRPAPFGNALVELAQRRPE
                     IVGMSADLAKYTDMHIFAQAFPERFFQMGMAEQLLMGAAGGMAKEGFIPFATTYAVFA
                     ARRAYDFIHKVIAEENLNVKIACALPGLTTGYGPSHQATEDLAIFRGMPNLTIIDPCD
                     AVDVEQAVPAMAAHNGPVYMRLPRGQVPVVLDEYNYTFDLGKAKLLRDGKDALIISSG
                     FMTMRALETVEKLR"
     gene            complement(699..1538)
                     /locus_tag="VLN25_05380"
     CDS             complement(699..1538)
                     /locus_tag="VLN25_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HSQ10030.1"
                     /translation="MTDKLSALADCAYRIRRYALRMGEVQGQGYIGQALGMAEILAVA
                     YGHALNLRPDDPEWEGRDRFLLSHGHYAIAFYAALIEAGIVPEEELETYGGDDSRLPM
                     SGMATYTPGMEISGGSLGQGLPIAVGMALGLRQKGNPAFVYNCMSDGELDEGSTWEAA
                     MSASHFQLANLITLVDVNNQQADGQSTKVLGFEPLVDKWAAFGWHVQRVSGNDLPAVL
                     AAFEKARNLAQPRPRVILFDTKMGKGVPFLEAREKNHFMQVAAAEWQHAIELLDAAHA
                     ARS"
     gene            complement(1580..>1843)
                     /locus_tag="VLN25_05385"
     CDS             complement(1580..>1843)
                     /locus_tag="VLN25_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSQ10031.1"
                     /translation="LGLGKAMARELGPDNIRVNSVTPGLIQTDITGGKLTPELKVEIL
                     KGIPLNRLGDAIDVSNCCLFLASDLSSYLTGVTLDVNGGMLIH"
BASE COUNT          312 a          598 c          579 g          354 t
ORIGIN      
        1 cgcagcttct cgaccgtttc cagcgcgcgc atcgtcatga agccgctgga aatgatcagc
       61 gcgtccttgc cgtcgcgcag cagcttcgcc ttgccaaggt cgaaggtgta gttgtattcg
      121 tcgagcacca ccggcacctg cccgcggggc aggcgcatgt acaccggccc gttgtgtgca
      181 gccattgccg gcaccgcctg ctcgacatcg accgcatcac acggatcgat gatcgtcagg
      241 ttcggcatgc cgcggaaaat ggcgagatcc tcggtggcct ggtgactcgg accgtaaccc
      301 gtggtcagtc cgggcagtgc gcaggcgatc ttgacgttga ggttttcctc ggcgatcacc
      361 ttgtgaatga agtcatacgc gcggcgcgcc gcgaataccg cataggtcgt ggcgaacggg
      421 atgaaacctt cctttgccat gccgccagcg gcgcccatca gcagctgctc ggccatgccc
      481 atctggaaga agcgctcggg gaatgcctgc gcaaagatgt gcatgtcggt gtacttggcc
      541 aggtcggcac tcatgccgac gatctcaggc cgccgctgtg ccagttcgac cagcgcattg
      601 ccgaacggag ccgggcgcgt gcgctgacct tcgccggcga tcgaggcgat catcgccgac
      661 gtcttcaggc gcggcttggt tgcaacggtg ctcatggttc agctccttgc cgcgtgcgcg
      721 gcatccagca gttcgatcgc gtgctgccac tcggcggcgg caacctgcat gaaatggttc
      781 ttctcgcgcg cttcgaggaa gggcacgccc ttgcccatct tcgtgtcgaa caggatcacg
      841 cgcggccgcg gctgcgccag gttgcgcgcc ttctcgaagg cagcgagcac cgcaggcagg
      901 tcgttgccgc tgacgcgctg tacgtgccag ccgaacgccg cccacttgtc cacgagcggt
      961 tcgaaaccca gtaccttggt cgattgacca tcggcctgct ggttgttgac gtccaccagc
     1021 gtgatcaggt tcgccagttg aaagtggctg gccgacatcg ccgcttccca cgtcgagcct
     1081 tcgtcgagct cgccgtcgga catgcagttg tagacgaagg ccgggttgcc cttctggcgc
     1141 agtccgagcg ccatgccgac tgcgatcggc aatccctgtc ccagcgagcc gcccgagatt
     1201 tccatacccg gcgtgtaggt cgccatgccg gacatcggca gtcggctgtc gtcacccccg
     1261 taggtttcca gctcctcctc cggcacgatg ccggcctcga tcagcgcagc gtagaacgcg
     1321 atcgcgtagt gcccatgcga cagcagaaag cggtcgcgac cctcccactc gggatcgtcg
     1381 ggccggaggt tcagcgcatg cccgtaggcg acggccagga tctcggccat gccgagcgcc
     1441 tggccgatgt acccctgtcc ctgcacttcg cccatccgca gcgcataccg gcggatgcga
     1501 taggcgcagt cggcaagcgc tgaaagcttg tcagtcatct gagatccttc gacagtgagt
     1561 gtcatgagtt ctgtcccggt cagtggatca gcattccgcc gttcacatcg agcgtgacgc
     1621 cggtgaggta gctggacaga tcactcgcga gaaacaggca gcagttcgaa acatcgatgg
     1681 catcgccgag ccggttcagc ggaatgccct tgagaatttc gaccttcagt tcgggcgtca
     1741 acttgccgcc ggtgatatcg gtctggatca gtccgggggt aaccgagttg acgcggatgt
     1801 tgtccggtcc gagctcgcgc gccatcgcct tgccgaggcc gag
//
LOCUS       DATIRT010000232         3817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1018522, whole genome shotgun sequence.
ACCESSION   DATIRT010000232 DATIRT010000000
VERSION     DATIRT010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018522"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="VLN25_05390"
     CDS             complement(<1..788)
                     /locus_tag="VLN25_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HSQ10032.1"
                     /translation="MAGARIAIARPPGSADALLLAQTAVQARGEQRPLVVVAADALDA
                     QRLVDEIPYFEPSLAVRPLPDWETLPYDTLSPHQDLVSDRLETLFRLSQAASSIDVLV
                     VPATTALYRLAPPQYVAGHTFFFRQGQSVDAEQLRAQLVFAGYQHVTQVVAPGEFAIR
                     GGLIDLFPMGSALPYRLDLLDAQLETIRAFDPDTQRGLYPVPEVRLLPGREFPIDEAA
                     RQAFRRRWRDRIEGDPSRSGVYKDIGSGIASAGIEYYLPLFFDET"
     gene            986..1687
                     /gene="ispD"
                     /locus_tag="VLN25_05395"
     CDS             986..1687
                     /gene="ispD"
                     /locus_tag="VLN25_05395"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HSQ10033.1"
                     /translation="MPRPLRYIALIPAAGVGVRMGAGDVPKQYLKLGERSMLEHSIDA
                     LLSDGRIDRVFVVVASTDTHWQEIAVDEERVEFLPVGGASRAESVRNGLIAIAARTGD
                     DDRVLVHDAARPCLNEEQLGRLIDEVGDDGSGGLLAIPLVDTLKRGEDGRVGITLERT
                     GLWCAQTPQLFRVASLRAALGGNVLDSITDEASALERAGHAPRLVLGGAANIKVTTAE
                     DLVLARAILAEQGRL"
     gene            1769..2251
                     /gene="ispF"
                     /locus_tag="VLN25_05400"
                     /pseudo
     CDS             1769..2251
                     /gene="ispF"
                     /locus_tag="VLN25_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123306.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
     gene            complement(2241..3023)
                     /locus_tag="VLN25_05405"
     CDS             complement(2241..3023)
                     /locus_tag="VLN25_05405"
                     /inference="COORDINATES: protein motif:HMM:NF025391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4241 domain-containing protein"
                     /protein_id="HSQ10034.1"
                     /translation="MSSAAFSRTRRALLATLGLGLSRVAHGRPVQSGNVIVEGGTPHQ
                     LPLAAVDDLGIEAPDLADDGPVDLAVLTLGAIEVPSGRLASTDGLLLEGAPFVQSIEP
                     GRYPLQVVLARLANGDERIAFVVVKLVDRAATTWTNAVIEGDDPAELQEDELSGIDVE
                     SGMACLFDATTLAAWRTELGRNEAMFRGLERVLRENRRPSWTWARVRAAGGSGYLFTS
                     GLGAGSYASYWGKDASGNLVSLAMDFDLLDWAGLEQATPVTA"
     gene            complement(3082..>3817)
                     /locus_tag="VLN25_05410"
     CDS             complement(3082..>3817)
                     /locus_tag="VLN25_05410"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSQ10035.1"
                     /translation="AVGPAEIRTVNESFNRMVTDLGKLEEDRAVLLAGISHDLRTPMT
                     RLRLEIEMADLQPETRGAMVGDLEQMDAIVRQFLDYARHTPQQPAERIDLSTLIEAAV
                     DRARIDGQAHASVNLLVAHDVSITGYRTELARALDNLLVNAVRYGRDPASGRLDLTIS
                     LSTDGQDAVLAVADRGPGVQAAHIDRLLRPFERGEEARSGSGGAGLGLPIVQRIARLH
                     GGSLRLLANAPHGLRAELTLPLERSA"
BASE COUNT          619 a         1265 c         1316 g          617 t
ORIGIN      
        1 gtctcgtcga agaacagcgg caggtagtac tcgatgccgg ccgatgcgat gccactgccg
       61 atgtccttgt agacgcctga gcggctcgga tcgccttcga tgcgatcgcg ccagcgccgg
      121 cggaaggcct gacgcgcggc ctcgtcgatc gggaactcgc ggcccggcag cagtcggact
      181 tcgggcacgg ggtacagccc gcgctgcgtg tccgggtcga aggcgcgaat ggtctcgagc
      241 tgggcatcga gcagatcgag ccggtagggc agggccgagc ccatcggaaa gagatcgatc
      301 aggccgcccc ggatcgcgaa ctcccccggt gcgaccacct gcgtgacgtg ctggtatccc
      361 gcgaacacca gctgcgcacg cagctgctcg gcatcgacgg actgtccctg gcggaagaaa
      421 aacgtgtgac cggcgacgta ctgcggtggc gcgaggcggt acagcgccgt ggtcgcgggc
      481 acgaccagca catcgatcga acttgctgcc tggctcaggc ggaacagggt ttcgagtcgg
      541 tcggagacca ggtcctggtg cggtgacagc gtgtcgtagg gcagcgtttc ccagtccggc
      601 agcggccgca ccgcaagcga cggctcgaag taggggattt cgtcgaccag gcgttgcgca
      661 tcgagtgcgt cggcggccac cacgacgagc gggcgctgct cgccgcgggc ctgcacggcg
      721 gtctgtgcca gcaaaagggc gtcggcagac ccgggcggcc gtgcgatggc aatacgggcc
      781 cctgccaccg gaagggagcg cggggcaagg cctgcgaagt ccgcgtcagg cgcggataac
      841 tggaagtcgt tgttcaagag aaaaaagacg gcagcaccga acccttcgat acaagggctc
      901 aagggctgcc gaactcggtc gttattatac gagtgccacc tttctctcga cggctgcacc
      961 gactcgcctt gcggattcca acttcgtgcc tcgacccctt cgctacattg cgttgatccc
     1021 ggctgccggt gtcggtgtcc ggatgggcgc gggtgacgtg cccaagcaat acctcaagct
     1081 gggcgagcgg agcatgctcg agcactcgat cgatgcgctg ctcagcgacg gccgcattga
     1141 tcgcgtcttt gtcgtcgtgg cgtcgaccga tacgcactgg caggagattg ccgttgatga
     1201 ggaacgcgtc gagttcctgc cggtcggtgg cgcatcgcgt gcggaaagcg tgcgcaacgg
     1261 actgattgcg atcgcggccc gcacggggga cgacgaccgc gtgctggtgc acgatgccgc
     1321 gcgcccatgc cttaacgagg agcaactggg ccggctgatc gacgaggtcg gcgacgacgg
     1381 cagcggtgga ctgctcgcca tcccgctcgt cgatacgctc aagcgcggcg aggacgggcg
     1441 cgttggcatc acgctcgagc ggacgggtct gtggtgcgcg cagacgccgc agctgtttcg
     1501 cgtggcatca ctgcgcgccg cactgggcgg caatgtgctc gactcgatca ccgacgaggc
     1561 cagtgccctg gagcgcgccg gtcacgcgcc gcgactcgtg ctgggcggag ccgccaatat
     1621 caaggtgacg accgccgagg acctggtgct ggcacgggcc atcctcgccg aacaggggcg
     1681 gctatgatgc ctcgcctttt cgctcatcgc gttatccgtc tggccatcga gcgttcatgg
     1741 accttcgggc ggcggctcgg gcaatgacat gacaagcttt cgcgtcggac agggtttcga
     1801 cctccatgcg ctggtgccgg gacgtcgact ggtcatcggt ggcgtcgaaa tcccgtttga
     1861 gcgcgggctc cttggccact ccgacgccga tgtgctgctg cacgcgatca cggacgccat
     1921 cctcggcgcc gccgggctcg gcgacatcgg ccagctcttt ccggacagcg atgagcgcca
     1981 ccgggatgct gacagccgct agttgctgcg cctggcgacc cagcgtgtgc gcgatgccgg
     2041 ctggcgagtc gaaaacgtcg acgcgaccgt gattgccgaa cgtcccagga tctcgcccca
     2101 tgtgcctgcg atgcgcgcag tgatcgccgc ctgcaccggt ctcgatcctc tggcggtcag
     2161 cgtcaagggg aagacgacgg agaagctcgg cgccaccggc cgaggtgagg gcatcgccgc
     2221 gcaggcggtg gcgctgcttg tcaggccgtg accggagtcg cctgctcgag cccggcccag
     2281 tcgagcaggt cgaagtccat cgccagggat accaggttgc cactggcgtc cttgccccag
     2341 taggacgcgt aggacccggc gcccaggccc gaggtgaaga ggtagccgga ccctcccgcg
     2401 gcacgcacgc gggcccaggt ccaggaaggc cgccggttct cgcgcagcac ccgttcaagg
     2461 ccgcggaaca tcgcctcgtt gcgtccgagc tccgtgcgcc atgctgccag cgtggtcgcg
     2521 tcgaacaaac aggccatgcc cgattcgacg tcgattcctg aaagctcgtc ttcctgcaat
     2581 tcggcgggat cgtcgccttc gatcactgca ttggtccagg tcgtggccgc acggtcgacg
     2641 agtttcacga cgacgaaggc gatgcgttcg tcgccgttcg ccagccgcgc gagcacgacc
     2701 tgcaacggat accgaccagg ctcgatggac tgcacgaacg gggcgccttc gagcagcaat
     2761 ccatccgtgc tggcgaggcg accactgggg acctcgatcg ccccgagcgt cagtacggcc
     2821 aggtcgaccg gcccgtcgtc cgcgaggtcc ggagcttcga ttccaagatc gtccaccgca
     2881 gccagcggca gctgatgcgg cgtaccgcct tcgacgatca cgttgcccga ctggacgggg
     2941 cggccgtgag ccactctgga caaacccagg ccgagcgttg ccagcagcgc ccgccgcgtc
     3001 cggctgaacg ccgcgctgct cacttggcgg ccccctttgg cgctgcgtaa cgacccacaa
     3061 ggcggagcgg tcgcgcagag ctcatgcgct gcgctcgagc gggagcgtca gttcggcgcg
     3121 cagtccgtgc ggtgcattgg ccagcagtcg aaggctgcca ccgtgcagac gcgcaatccg
     3181 ttgcacgatc ggcagcccta gccccgcgcc accgctgccg ctgcgggctt cctcgccgcg
     3241 ctcgaacggg cgcagcaggc gatcgatgtg cgcggcctgc acccctggcc cccgatcggc
     3301 gacggccagg acggcgtcct gtccgtcggt cgacagcgaa atcgtgaggt cgagccgacc
     3361 ggacgccgga tcgcgcccgt agcgcaccgc attgacaagc aggttgtcga gcgcgcgggc
     3421 aagctcggtg cggtatccgg tgatgctcac gtcgtgagca acgagcagat tgacggaggc
     3481 gtgcgcttgt ccgtcgatgc gcgcgcgatc gaccgcggct tcgatcaatg tcgaaaggtc
     3541 gatccgctcc gccggctgct ggggcgtgtg ccgcgcgtag tcgaggaact gacgcacgat
     3601 cgcgtccatc tgttcgaggt cgccgaccat cgcgccgcgg gtttccggtt gcaggtccgc
     3661 catctcgatt tccaggcgca gtcgcgtcat cggcgtgcgc aagtcgtgcg agatccctgc
     3721 cagcagcacg gcacgatcct cctcgagctt gccgaggtcc gtcaccatgc gattgaagct
     3781 ttcattcacc gtgcggatct cggccggccc gacggcg
//
LOCUS       DATIRT010000233         2673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1411840, whole genome shotgun sequence.
ACCESSION   DATIRT010000233 DATIRT010000000
VERSION     DATIRT010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411840"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2346)
                     /locus_tag="VLN25_05415"
     CDS             complement(<1..2346)
                     /locus_tag="VLN25_05415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSQ10036.1"
                     /translation="MDPIRSPSASSLPPDAVLLAFDRMGRAHARRVDFTRFVPLLAIA
                     LVVALSAGLAYLLVNEDRALQRDALHRDTDTLAQSLSLRLQGISEATNALAREAASAD
                     RTERRFVAAAREAMVVKSEIVHVAVVDDAVRVVWSAASPGPLGESVRTPNTQLTSDRL
                     VGAISNARNAHSSFFSPVYDDPSGSAGPDNGGPFTDLVAPIFVEDLYRGAVIARVSLP
                     ELLRRSVAPDVAMRYRVALLNDAGDAVASTTASETPRDALVYQVPIYLLPYGVSLQAS
                     AFRVRSPLLGNALAWVVAGLTIAVLVTLVALMRRDAKQARVERALRAETAFRRAMEDS
                     LATGLAVIDRHGVIRHVNAAFCQMTGWDLPELVGRSAPYPYWPPGQFADHQATLRALL
                     EGKAPASGFELKVLRRDGSLFDARLYVSPLLSDDGEQLGWMTAMADITEPKRISEQLE
                     AAHERFTTVLQSLDDAVSVVAVGGSESPDPAADGLLFANRGYKKLYGSTALGHLRLQM
                     SLAANGSAPAGEVYDSELKRWFDVRTREVRWVDGRAVRLQIATDVSDRKATEEIVRQQ
                     QEKVQLTSRLMTMGEMASSLAHELNQPLTAISNYSLGTVSRLRGGNVAAADLLPALEK
                     TAGQAQRAGNIIRRIREFVKRAEPRRRPTPISRIIEDAIGFAEIEAAKKKIAIRTQVP
                     TDLPPLDVDPILVEQLLLNLLKNAIDATDNAAVRRIDVVVRRLDDMAEFAVIDRGCGV
                     PVEHRASLFQPFFSTKPDGMGMGLNICRSIVEFHQGRLQLEE"
BASE COUNT          486 a          866 c          868 g          453 t
ORIGIN      
        1 ttcctcaagc tgcaggcgtc cctggtggaa ctcgacgatg gatcggcaga tgttgaggcc
       61 catgcccatg ccatccggct tcgtgctgaa aaatggctgg aaaaggctcg ctcgatgctc
      121 aaccggcacg ccacagcccc ggtcgatgac cgcgaattcg gccatgtcgt cgagccggcg
      181 gacgaccacg tcgatacggc gcacagctgc gttgtccgtc gcatcgatcg cattcttaag
      241 caggttcagc agcagctgct cgaccaggat cggatcgaca tcgagcggcg gcaagtccgt
      301 cggtacttgc gtgcgaatcg cgatcttctt cttggcggct tcgatctcgg cgaatccgat
      361 cgcgtcctcg atgatgcgtg atatgggggt ggggcgtcgg cgcggctccg cgcgcttcac
      421 gaactcgcgt atgcggcgaa tgatgttgcc ggcgcgctgt gcctggccag ccgtcttttc
      481 cagcgcaggc agcaggtctg ccgctgcgac gttgccgccc cgcaggcgcg agaccgtccc
      541 caggctgtag ttgctgatcg cggtcagcgg ctggttcagt tcgtgcgcga gcgatgacgc
      601 catctcaccc atcgtcatca ggcgtgatgt cagctggact ttctcctgct gctgccggac
      661 gatttcctca gtggccttgc ggtcgctgac atcggtggcg atctgcagtc gcacggcacg
      721 gccatcgacc cagcgcacct cgcgggtccg cacgtcgaac cagcgtttca gttcgctgtc
      781 gtagacctcc cctgcgggcg cgctgccgtt ggccgcgagc gacatctgca gccgcaagtg
      841 ccccagtgcg gtcgacccgt agagtttctt gtagccccgg ttggcaaaca gcagcccgtc
      901 ggcagcggga tcaggcgatt ccgaccctcc gacggcgacg acggaaacgg cgtcgtcgag
      961 cgactgcagc accgtagtga accgttcgtg cgcggcctcg agttgctcac tgatgcgctt
     1021 gggttcggtg atgtcggcca tggccgtcat ccagccgagt tgttcgccat cgtccgaaag
     1081 cagcggcgac acgtacagcc gggcatcgaa cagtgagccg tcgcgtcgca gtactttcag
     1141 ctcgaagccg ctcgcgggcg ccttgccctc gagtaacgcc cgcagcgtcg cctggtggtc
     1201 ggcaaactga ccgggcggcc agtacgggta cggggcgctg cgcccgacca gttcgggtaa
     1261 gtcccatccc gtcatttggc agaaagccgc attaacgtgc cggatgaccc catgacggtc
     1321 gatgaccgcc aggcccgtcg ccagcgagtc ttccattgca cgtcggaagg ccgtctcggc
     1381 gcgcaacgcg cgctcgacgc gcgcctgctt ggcgtcgcgc cgcatcagtg cgacgagcgt
     1441 gaccagaacg gcgattgtca ggccggcgac cacccacgcg agcgcattgc ccagcaacgg
     1501 cgagcgcacg cgaaacgccg atgcctgcag cgagacaccg tacggcaaca ggtagatcgg
     1561 cacctgatag acgagggcat cacgcggtgt ctcactcgcc gtcgttgagg ccactgcgtc
     1621 gccggcgtcg ttcagcagag cgacgcggta gcgcatggct acgtccggcg cgacagatcg
     1681 ccgcagcagc tccggcaatg acaccctcgc aatcaccgcg ccgcgataca gatcctcgac
     1741 aaagatcgga gcgaccaggt cggtgaacgg gccgccgttg tccggaccgg ccgagcccga
     1801 gggatcgtca tagaccggcg aaaagaacga cgaatgcgcg ttgcgtgcat tgctgatggc
     1861 gccaacaagg cggtccgagg tcagttgcgt gttcggggtt cgcaccgatt cgccgagcgg
     1921 acccggggac gccgcagacc acacgacgcg cacggcatcg tcgacgaccg cgacgtggac
     1981 gatctccgat ttgacgacca tggcttcgcg cgcggccgcc acgaagcggc gctctgtccg
     2041 atccgcgctc gcggcctcgc gcgccagagc attcgtcgct tcgctgattc cctggagccg
     2101 cagggacagt gactgtgcca atgtgtccgt gtcacgatgc aaggcgtcgc gctgcagggc
     2161 gcggtcttcg ttcaccagca ggtaggcgag cccggcactg agcgccacga ccagcgcaat
     2221 cgccagcagc ggaacgaagc gtgtgaaatc gacgcgtcgc gcgtgcgcgc gccccatgcg
     2281 atcgaacgcg agcaggaccg cgtcgggcgg cagcgatgag gcagaagggg agcggatggg
     2341 gtccacgggc acgagggcag atcatcccat cggaacccgg cgcgcatatt atcgagagcg
     2401 cttgcagcgc attcaggacc gtgtgtgcac tatggaagtt ccgcatagca aaacgtaaca
     2461 ccataatttg aaagattttg gcgatgctgt tatcgtcgcc tttcactaca aggccgcgcc
     2521 caagtcgcga ggccgttcgg tttgatgagc tggacccatc gatcaggaga caacgatgtc
     2581 cgccattccc aaggctgcca acgacgccga cacgctcgaa acccaggaat ggatcgaagc
     2641 gctcgaaacg gtgctcgagc gcgaaggtgc gca
//
LOCUS       DATIRT010000234         4857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_919927, whole genome shotgun sequence.
ACCESSION   DATIRT010000234 DATIRT010000000
VERSION     DATIRT010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919927"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VLN25_05420"
     CDS             <1..220
                     /locus_tag="VLN25_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HSQ10037.1"
                     /translation="LFDEETHRIVGGGIVGTHAGDLISEVALAIEMGADAVDIGRTIH
                     PHPTLGESIGMAAEVAEGACTDLPPPRR"
     gene            400..1569
                     /locus_tag="VLN25_05425"
     CDS             400..1569
                     /locus_tag="VLN25_05425"
                     /inference="COORDINATES: protein motif:HMM:NF021665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G8 domain-containing protein"
                     /protein_id="HSQ10038.1"
                     /translation="MGFLEFQAAGAPPVPADIFPEGAQRWSNPSTWGGRIPADGAPVH
                     IEAGRTVVLDQDIDVASLDVEGTLLLSTRDAKVRAGWILVHGNGRLAAGSPERPFREQ
                     LSFTLCARDGVIPHPALGSKFLAALDGGTIDLHGEQRTSWVPLGSSVAPGHNTIRLAQ
                     PVDWRVGERLAIASGHDHALVEERTIVDVGGDCMTLMLDRAVAHRHHGQHAPLLHMQP
                     GSIAKAVLLERNIVVQGCEESERASQGGYCMIASSLQPGTAGRRAIGRFSGVEFRRMG
                     QFNRPGRYPLHWHNNGDAAESSVSGCLVQHSYQRGIVTVGSRHVRICGNVVLKPYGHG
                     YIVESEDNSPQLVTTNLAVRPRVARFADRAMRSYCEHRPRPFWIVRTQPVRPAAH"
     gene            1621..2286
                     /gene="rpiA"
                     /locus_tag="VLN25_05430"
     CDS             1621..2286
                     /gene="rpiA"
                     /locus_tag="VLN25_05430"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HSQ10039.1"
                     /translation="MATQDELKRAVARAAIRHVPDGEIIGVGTGSTADFFIDELATIR
                     SRIKGAVASSERSAQRLATLGIPLFDLNDVEALAVYVDGADEIDPGFSMIKGGGGALT
                     REKIVAAVARTFVCIADESKLVRVLGQFPLPVEVIPMAGAQVARELVRLGGSPRQRAD
                     FVTDNGNVIIDVHGLRIDAPAAFEQRIDGIVGVVTNGLFARRGADVLLLGTPDGVRET
                     RRA"
     gene            2283..2969
                     /locus_tag="VLN25_05435"
     CDS             2283..2969
                     /locus_tag="VLN25_05435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02117 family protein"
                     /protein_id="HSQ10040.1"
                     /translation="MNAWLRGAGLAGAALFLMLTGYVIAALVLGALPRNTDFVESHDG
                     VPVYVRTNGVHAELIVPTRHGDVDWSAEFPVRQMRALPAPTDWIAFGWGDRGFMVNTP
                     TWSDLHPTTAVVALSGLGDGAMHVEYVETPTAYKAKRVRLSAAEYARLVAYIRASFVR
                     DPDGQVRQIDAPGYFDSDAFYEAVPTYAFWYTCNEWTRRALVAAGVRTAFWAPFDTAV
                     MYHLPLPPAR"
     gene            complement(3013..3285)
                     /locus_tag="VLN25_05440"
     CDS             complement(3013..3285)
                     /locus_tag="VLN25_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HSQ10041.1"
                     /translation="MARMIHCVKLGREAEALDFPPVPGDLGKRIWENVSKEAWAGWLK
                     HQTMLVNENRLNLADARARKYLLTQMEKHFFGEGADAAAGYVPPSS"
     gene            complement(3297..4658)
                     /gene="argA"
                     /locus_tag="VLN25_05445"
     CDS             complement(3297..4658)
                     /gene="argA"
                     /locus_tag="VLN25_05445"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HSQ10042.1"
                     /translation="MNDAALNDAAVKQPLVRATDQAQFVAWLRQVAPYVHAHRGRTFV
                     IAFSGSLIEAGQLTTLIQDVSLLNAMGIRIVLVHGSRPQVQAQLALKGLDARFHNDVR
                     VTDAAALECVKEAAGEIRLDIEAAFSQGLPSTPMAHSKVRVVSGNFVTARPMGIIDGV
                     DLQLSGVVRKVDTDALRFVLANGGLVLLSPLGFSPTGEAFNLTMEDVACSVATALKAD
                     KLVLLTETAGIAGTEGQMLNELSVEDAERMLTAGEVTGDAAFNLRYAVKACRAGVARA
                     HILSYSLDGGVLVELFTHDGVGTMVAPENLEKLRDATADDVGGVIKLIEPLEADGTLV
                     KRPRELIEREIDRFTVLEHDGFIFGCAALYPFAGEKTGELACLTVHPQYQGYGDGERL
                     MKRIEVRAKAAGIERLFVLTTRTAHFFIKRGFKPATVDDLPPTRQNMYNWQRRSQVLI
                     KSI"
BASE COUNT          779 a         1610 c         1632 g          836 t
ORIGIN      
        1 gctgtttgac gaagagacgc atcgcatcgt cggtggcggc atcgtcggga cgcacgccgg
       61 cgacctgatc agcgaagtcg ccctggcgat cgagatgggc gccgacgcgg tcgacatcgg
      121 ccgcaccatc catccgcatc ccaccctcgg cgaatcgatc ggcatggccg cggaagtcgc
      181 cgaaggcgcc tgcacggacc tgccgccgcc gcgtcgctga acgcctctgc aggcgctgtc
      241 gatggccgga aatgccggcg ctaagtgcgg cgccgggtgc cgtcatcctt ccggatgcgg
      301 gcagcgcgat tgacgctccg cggtggcgtc gtacccttcg tgttcagacg agcgcggccg
      361 tgttggcagc gccgacagag accaccgcat cgagcacaga tgggcttcct cgaattccag
      421 gccgccggcg cgccgccggt gccggccgac atcttccccg agggtgcgca gcgctggtcc
      481 aatccctcca cctggggggg acggatcccc gcggacggtg cgccggtgca tatcgaggca
      541 ggccggaccg tcgtgctgga tcaggacatc gacgtggcca gcctcgatgt cgaaggaacc
      601 cttctactct ccacacgcga cgcgaaagtg cgtgccgggt ggatactggt ccatggcaat
      661 ggccggttgg ccgccggcag tccggagcgg ccgttccgcg agcaactgag ctttacgcta
      721 tgtgcccggg acggtgtgat tccccacccg gcacttggca gcaaattcct cgccgcgctc
      781 gacggcggca ccatcgacct gcatggcgag cagcgcacct cctgggtacc gctgggcagc
      841 agcgtcgcgc cgggacacaa cacgatccgg ctggcgcagc cggtggattg gcgcgtcggt
      901 gaacggctcg ccatcgcctc cgggcacgat catgcgctgg tcgaagagcg cacgattgtc
      961 gatgtcggcg gagactgcat gacgctgatg ctcgatcgag cggttgccca tcgtcatcat
     1021 ggtcagcatg cgccgctgct gcacatgcag ccgggttcga tcgccaaagc ggtgctgctc
     1081 gaacgcaata tcgtcgtcca gggctgcgaa gagtcggagc gcgcgagcca gggcggctat
     1141 tgcatgatcg ccagttcgct tcagcccggc accgcggggc ggcgtgcgat cggacgcttc
     1201 tccggcgtgg agttccggcg catgggacag ttcaatcgcc cgggccgcta tccactgcac
     1261 tggcacaaca acggcgatgc ggccgaaagt tccgtcagtg gttgcctcgt gcagcattcc
     1321 tatcagcgcg ggatcgtcac ggtcggttcg cgccatgtgc gcatctgcgg caacgtcgtg
     1381 ctcaagccgt acggacacgg ctatatcgtc gagagcgagg acaactcgcc gcagctggtc
     1441 acgaccaatc ttgcggtacg cccgcgcgtg gcgcggttcg cggacagggc gatgcgcagc
     1501 tattgcgaac atcgaccgcg gcccttctgg attgtccgta cacagccggt gcggccggcg
     1561 gctcactagc ccttcggcgc gcccgcagtc gctatcatcc cggccgttgc aaggaaggca
     1621 atggctaccc aggacgaact caagcgggcg gtcgcgcgcg ccgcgatccg tcatgtgccg
     1681 gacggcgaga tcatcggtgt cggaaccgga tcgaccgccg atttttttat cgatgagctc
     1741 gcgacgatcc ggtcgcgcat caagggcgca gtcgccagct cggagcgcag tgcccagcgg
     1801 ctcgcgacgc tcgggatccc gctcttcgac ctcaacgacg tcgaagcgct cgccgtgtac
     1861 gtcgacggcg ccgacgagat cgatcccggc ttttcgatga tcaagggggg cggcggtgcg
     1921 ctgacccgcg agaagatcgt cgcggccgta gcgcgcacct ttgtctgcat tgccgatgag
     1981 agcaagctcg tgcgcgtgct cggccagttc ccgctgccgg tcgaggtcat cccgatggcg
     2041 ggggcgcagg tcgcccgcga actcgtgcgg ctcggcggat cgccgcgaca gcgcgcggat
     2101 ttcgtgactg acaacggcaa tgtcatcatc gacgtccacg ggttgcggat tgatgccccg
     2161 gcggctttcg agcaacgaat cgacggcatt gtcggagtcg tgaccaacgg gctctttgcg
     2221 cgaaggggag cggacgtgct gctgctcggc acaccggacg gggtccggga aacgcggcga
     2281 gcatgaacgc ctggctgcgc ggagcggggc tggccggcgc ggcgctgttc ctaatgctca
     2341 ccggctacgt gatcgcggcg ctggtgctcg gtgcgctgcc gcgcaatacg gacttcgtcg
     2401 agtcccatga cggtgtcccg gtgtacgtgc ggaccaatgg cgtgcatgcg gaactgatcg
     2461 tgccgacgcg gcacggcgac gtcgactgga gcgccgaatt tccggtgcgg cagatgcgcg
     2521 cactgccggc gccgacggac tggattgcat tcggctgggg cgaccgtggc ttcatggtca
     2581 atacgcccac ctggtcagac ctccacccaa cgaccgccgt tgtcgccctc agtggtctgg
     2641 gtgacggcgc gatgcacgtc gagtacgtgg aaacgccgac ggcctacaag gccaagcgag
     2701 ttcgcctgtc agccgcggag tacgcgcggt tggtcgccta catccgtgcc tctttcgtac
     2761 gtgacccgga cgggcaggtg cgccagatcg atgcgccggg atacttcgac tccgacgcgt
     2821 tctacgaggc ggtgccgacg tatgcgttct ggtacacgtg caacgagtgg acgcgccgtg
     2881 ccctggtggc tgccggcgtt cggaccgcct tctgggcgcc gttcgatacg gccgtgatgt
     2941 accacctgcc gctgcccccc gcgcgctgag ccgcacggta ctgatagcag cggcgctcgg
     3001 ggagtcgccc gtctagctcg acggcggcac gtagcccgcc gcggcgtcgg cgccttcgcc
     3061 aaagaagtgc ttttccatct gcgtcagcag gtacttgcgg gcgcgggcat cggccaggtt
     3121 gaggcggttc tcgttgacca gcatcgtctg gtgcttgagc cagccggccc acgcttcctt
     3181 cgacacgttc tcccagatcc gcttgccgag gtcgcccggg accggcggga agtcgagggc
     3241 ctcggcctcg cggccgagct tcacgcagtg aatcatgcgc gccatggtgc gttctcctaa
     3301 atggacttga tcagcacctg cgagcggcgc tgccagttgt acatgttctg tcgcgtcggc
     3361 ggtaagtcgt cgacggtggc cggcttgaat ccgcgcttga tgaagaagtg cgcggtacgg
     3421 gtggtcagca cgaaaagacg ttcgatgccg gcggctttgg cccggacctc gatgcgcttc
     3481 atcaggcgct cgccgtcgcc gtagccctga tactgcgggt ggaccgtgag acatgcgagt
     3541 tcgccggttt tctcgcccgc gaacgggtat agcgccgcgc acccgaagat gaagccatcg
     3601 tgctcgagca cggtgaagcg gtcgatctcg cgctcgatca gctcgcgcgg ccgcttgacg
     3661 agcgtgccgt cggcctcgag gggctcgatc agcttgatga cgccgccgac gtcatcggca
     3721 gttgcatcgc gcaacttctc aaggttctcc ggcgccacca tcgtgccgac gccgtcgtgc
     3781 gtgaacaact cgaccagcac gccgccatca agcgaatagg aaaggatgtg cgcccgcgcg
     3841 acgccggcgc ggcaggcctt gaccgcatag cgcagattga aggcggcatc gcccgtgacc
     3901 tcgccggcag tcagcatgcg ctccgcgtcc tccacggaca gttcgttcag catctgaccc
     3961 tccgttccgg caatgccggc ggtctcggtc agcaacacga gcttgtcggc cttgagtgcg
     4021 gtcgcgacgc tgcaggccac gtcttccatc gtgaggttga acgcttcgcc ggtcggcgag
     4081 aaaccgagcg gcgagagcag taccagcccg ccgttcgcga gtacgaagcg cagcgcgtcc
     4141 gtgtcgacct tgcgcacgac gccgctcagt tgcagatcga cgccgtcgat gatgcccatg
     4201 ggccgggccg tgacaaagtt gccgctgacg acccgcactt tcgagtgggc catcggcgtc
     4261 gatggcaggc cctgcgaaaa tgcggcctcg atgtccagcc gaatctctcc cgcggcttcc
     4321 ttcacgcact cgagcgcggc ggcgtccgtg acacgaacgt cgttgtggaa ccgggcgtcg
     4381 aggcccttga gtgcgagctg cgcctgcacc tgaggccgcg agccgtgcac cagcacgatc
     4441 cggattccca tggcgttcag cagcgatacg tcctggatca gcgtggtcag ttgtccggct
     4501 tcgatcaggc tgcccgaaaa cgcgatcacg aaagtgcgtc cgcgatgcgc atggacgtag
     4561 ggcgcgacct gcctcagcca ggcgacaaac tgcgcctggt cggtggcccg gacaagcggt
     4621 tgcttcactg cggcgtcgtt cagcgcggca tcgttcatca gcgaaagtat actttcgctg
     4681 tttccttcac cgcgcattca ccgagaatcc gccgcgatcg agcgcactcc gtgcgcgtgc
     4741 ggcgcccctg gccacgagac tcccatgacg cgctagcacc gcgaggtgcg acgcgagccg
     4801 atgcagcccc aaccgtccaa cccgccccgc tcaagggggc cacgggttcg cgcgccg
//
LOCUS       DATIRT010000235         2170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1117266, whole genome shotgun sequence.
ACCESSION   DATIRT010000235 DATIRT010000000
VERSION     DATIRT010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117266"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VLN25_05450"
     CDS             complement(<1..386)
                     /locus_tag="VLN25_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HSQ10043.1"
                     /translation="MTQEQKIKVVRANGGKVANGQMLKFNVMRFNPADPASVPHLQTY
                     ELEQTDGMTLFIALNEIREKLDPSLQFDFVCRAGICGSCAMVINGRPGLACRTLAKDV
                     GPEITLAPLPVFELIGDLSVNTGKWMR"
     gene            complement(383..>2170)
                     /locus_tag="VLN25_05455"
     CDS             complement(383..>2170)
                     /locus_tag="VLN25_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase flavoprotein subunit"
                     /protein_id="HSQ10044.1"
                     /translation="DTVRGSDWGADQDVVRMFVNTAPKAVRELAAWGVPWSRVRKGDR
                     QVVINSQKVTITERDEAHGLVAQRDFGGTKKWRTCYVADGTGQAMLQAVGDQAIAALI
                     PVHERTEALALIHDAGRCYGAVVRNLITGELTAYVAKATEIATGGAGRIYRVTTNAVI
                     CEGIGHAIALETGVAALGNMEAVQFHPTGIFPAGILVTEGCRGDGGLLKDVDGHRFMP
                     DYEPEKKELASRDVVSRRMEEHIGKGKGVKSRFGEHIWLDITQLGEAHIHRNLREVFD
                     ICHYFLGVDPTKEMIPVRPAQHYTMGGVRTNHTGESPTLKGLFAAGEASCWDMHGFNR
                     LGGNSVAETVVAGMIVGEYIADFCDKTENDVNIPTGVVREFLQREQAKLNGILLGRGS
                     EDPSAIKARMQEIMTAKVGIFRRGEDLESAVDELQKLLVRSRNIGLRSNAPGVNPELV
                     TAYRTQMMLKLAVTVAYGALVRTESRGAHFRQDHPRRNDAEWLKRTLATWKSESDTLP
                     TLNYEPLDIKRMELPPGWRGYGAKDYVDHPDTAARQAEVDAVKQKIGDRFDLQKTLMP
                     YEHLLPEKFRGRNERIDEPLANAPVRRTA"
BASE COUNT          306 a          724 c          710 g          430 t
ORIGIN      
        1 cgcatccact tgccggtgtt gaccgacagg tcgccgatca gctcgaacac cggcaatggc
       61 gccagcgtga tttccggtcc gacgtccttg gccagcgtgc ggcaggccag ccccggccgg
      121 ccgttgatga ccatcgcgca gctgccgcag atgccggcgc ggcagacgaa gtcgaactgc
      181 agcgacgggt cgagcttctc gcggatctcg ttgagcgcga tgaacagcgt catcccgtcg
      241 gtctgctcga gttcgtaggt ctgcaggtgc ggaacactgg ccgggtcggc cgggttgaac
      301 cgcatcacgt tgaacttgag catctggccg ttggcgacct tgccaccgtt ggcacggacc
      361 accttgatct tctgttcctg cgtcatgctg tcctccgcac gggcgcgttg gcgagcggct
      421 cgtcgatccg ttcgttgcgg ccgcggaatt tctcaggcag cagatgctcg tacggcatca
      481 gcgtcttctg caggtcgaag cggtcgccga tcttctgctt cactgcgtcg acctcggcct
      541 ggcgtgccgc tgtgtccgga tggtcgacat aatccttggc cccgtatccg cgccagcccg
      601 gcggcagttc catgcgcttg atgtcgagcg gctcgtagtt gagcgtcggc agcgtgtcgg
      661 actcgctctt ccaggtcgcc agcgtgcgtt tcagccactc ggcgtcgtta cggcgcggat
      721 gatcctggcg gaagtgcgcg ccacggctct cggtgcgcac cagcgcgccg taggcgaccg
      781 tcacggcgag tttcagcatc atctgtgtgc gataggcggt cacgagttcc ggattcacgc
      841 ccggtgcgtt tgaacgcagg ccgatgtttc gactgcggac gagaagcttc tgcagttcgt
      901 cgacggcgga ctcgagatcc tcgccgcgcc ggaagatgcc gaccttcgcg gtcatgatct
      961 cctgcatgcg ggccttgatc gcactcggat cctcgctgcc ccggccgagc aggatgccat
     1021 tgagcttggc ctgctcgcgc tggaggaact cgcgcacgac gccggtcggg atgttgacgt
     1081 cgttctcggt cttgtcgcag aagtctgcga tgtattcgcc gacgatcatg ccggccacca
     1141 cggtttcggc caccgaattg ccgccgagcc ggttgaaacc gtgcatgtcc cagcacgaag
     1201 cctcgccggc cgcgaacagg cccttcagcg tcgggctttc gcccgtgtga ttggtgcgca
     1261 cgccgcccat cgtgtagtgc tgggccggcc gcaccggaat catctccttc gttggatcga
     1321 cgccgaggaa gtagtggcag atgtcgaaga cctcgcgcag attgcggtgg atgtgagcct
     1381 cgccgagctg cgtgatgtcg agccagatgt gttcgccgaa gcgcgacttg acgcccttgc
     1441 ccttgccgat gtgctcctcc atccggcggc tcacgacgtc gcgtgaggcg agctccttct
     1501 tctccggctc gtagtccggc atgaagcggt ggccgtcgac gtccttcagc agtccgccgt
     1561 cgccgcggca gccttcggtc acgaggatgc cggccgggaa gatgcccgtc gggtgaaact
     1621 gcaccgcctc catgttgccc agcgccgcga cgccggtttc cagcgcgatc gcgtggccga
     1681 tgccttcgca gatcaccgca ttggtcgtca cgcgatagat cctgccggcg ccgcccgtgg
     1741 cgatctcggt cgccttggcc acgtaggccg tgagttcgcc ggtgatcaga ttgcgcacga
     1801 cggcgccgta gcagcggccg gcgtcgtgga tcagcgccag cgcctcggtg cgctcgtgca
     1861 ccgggatcaa cgcggcgatg gcctggtcgc cgaccgcctg cagcatcgct tgtccggtgc
     1921 cgtcggcgac gtagcaggtg cgccacttct tggtgccgcc gaaatcgcgt tgcgcgacca
     1981 ggccatgggc ctcgtcgcgc tcggtgatgg tcactttctg gctgttgatc acgacctgac
     2041 gatcgccctt gcgcacgcgg ctccatggca cgccccaggc cgccagttcg cgcacggcct
     2101 tcggtgcggt gttgacgaac atgcggacca cgtcctgatc ggcgccccag tcactgccgc
     2161 gcaccgtgtc
//
LOCUS       DATIRT010000236         3372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1608585, whole genome shotgun sequence.
ACCESSION   DATIRT010000236 DATIRT010000000
VERSION     DATIRT010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1608585"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            376..804
                     /gene="rplM"
                     /locus_tag="VLN25_05460"
     CDS             376..804
                     /gene="rplM"
                     /locus_tag="VLN25_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HSQ10045.1"
                     /translation="MKTFSAKSHEVKRDWLLVDANDKVLGRLASQIALRLRGKHKPEY
                     TPHVDTGDFIVVVNAAGLKVTGAKFEDKKYYRHTGYPGGVYETNFRKMQERFPGRALQ
                     LAVKGMLPKGPLGYAMIKKLKVYADQQHPHSAQQPKALDI"
     gene            812..1204
                     /gene="rpsI"
                     /locus_tag="VLN25_05465"
     CDS             812..1204
                     /gene="rpsI"
                     /locus_tag="VLN25_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HSQ10046.1"
                     /translation="MIGNYNYGTGRRKSSVARVFLKRGKGGIVINGRPIEQYFFRETG
                     RMIVRQPLELTNHAETFDISVNVNGGGESGQAGAVRNGITRALIDYDATLKPTLSNAG
                     LVTRDAREVERKKVGFRKARRRKQFSKR"
     gene            1503..2237
                     /locus_tag="VLN25_05470"
     CDS             1503..2237
                     /locus_tag="VLN25_05470"
                     /inference="COORDINATES: protein motif:HMM:NF042896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HSQ10047.1"
                     /translation="MKWKLWLRNLSVSAPRVAVRSQLPWALRAILALILAALAAAALG
                     AMYHYARNVRTPDRSEILSELETAREQFRQAAAERERFAARAVQAENQLKVERVMQEQ
                     IALQMKTVEEDNARLKGDLAFFESLLPTASGARGVVIRSFKLQPDVDDNTVRYRLLVQ
                     QSGRPERDFVGAVVIKVNLMHDGRADVLQLPDAGHPAAGPAPLVFRHYQRLEGTFAVP
                     AGATVRSVQVTINAGGETRAQQTFSM"
     gene            2247..2660
                     /locus_tag="VLN25_05475"
     CDS             2247..2660
                     /locus_tag="VLN25_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HSQ10048.1"
                     /translation="MFGPKRASPKTIGTLVGVGTTIEGTLSYTGGLRIDGTVKGDVRS
                     VNGEQGMVIISEQGNVIGEVHAAHLVVSGKVAGPLFVTDILELQPKAQVQGDVHYRAL
                     EIHHGAVVEGRLIHDIPDARPALKLAAGGTTSEAE"
     gene            2723..3073
                     /gene="erpA"
                     /locus_tag="VLN25_05480"
     CDS             2723..3073
                     /gene="erpA"
                     /locus_tag="VLN25_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HSQ10049.1"
                     /translation="MNAAVEMPAPLVFSDAAASKVKALIDEEGNPGLKLRVFVQGGGC
                     SGFQYGFTFDEVINEDDATMEKNGVMLLIDSMSYQYLVGAEIDYKEDLEGAQFVIKNP
                     NATTTCGCGSSFSA"
     gene            complement(3147..>3372)
                     /locus_tag="VLN25_05485"
     CDS             complement(3147..>3372)
                     /locus_tag="VLN25_05485"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HSQ10050.1"
                     /translation="CGGGTRNRALMGDLHALLAPRSVMSSDALGIPPDQVEALAFAWL
                     ARRCIRRETGSLPSVTGARGARVLGAIYPR"
BASE COUNT          666 a         1015 c         1083 g          608 t
ORIGIN      
        1 cccaaggctc acgccttggg ccaatttttt ggtcgcttgc ggaaggcgtc caaaaacgct
       61 taaaatttaa ggctttccgc gatctgcttg ccatactttt ttgtgcagcg cgaactttgg
      121 gccgcgcgaa cccgatggca acggaccgtt gcgatcgagt gaagcgctac caatcgggtt
      181 tttcgggctc gcgaactgcg gcttcggatg gccggagcgg atttctccgg atgcaatgcg
      241 gaattcaagg cggtcaaccg gcccaaggcg gtcaaccggc cgccaggcat gcaaacggaa
      301 tttttccgcc gtgatggccc gaaagttgaa cgcgggtgct acggcgctgt gaccaagttt
      361 caggatggat cgtcaatgaa aaccttttct gccaagtccc acgaggtcaa gcgcgactgg
      421 cttctggtcg acgccaatga caaagtgctc ggtcggctcg cgagccagat cgcattgcgc
      481 ctgcgaggca agcacaagcc ggagtacacg ccgcacgtcg acaccggtga cttcatcgtc
      541 gtcgtgaacg ccgccggcct gaaggttacc ggtgcgaaat tcgaagataa gaagtactac
      601 cgccacaccg gctatccggg cggcgtgtac gagaccaact ttcgcaaaat gcaggagcgc
      661 tttccggggc gcgcgctgca gctggcggtg aagggcatgc ttcccaaggg tccgctcggc
      721 tacgcgatga tcaagaagct gaaggtctat gccgaccagc agcacccgca cagcgcgcag
      781 caacccaaag cgcttgacat ctgaggctca catgatcggc aattacaact acgggaccgg
      841 ccgccgcaag agttcggtag cgcgcgtctt cctgaagcgc ggcaagggcg gcattgtgat
      901 caacggccgt cccatcgagc agtacttctt ccgcgagacg ggtcgcatga tcgtgcgcca
      961 gccgctggaa ctgacgaacc atgccgagac gttcgacatc agtgtcaacg tcaacggcgg
     1021 tggcgagtcg ggccaggcag gtgctgtgcg caacggaatc acccgcgcgc tgatcgatta
     1081 cgacgcaacg ctcaagccga cgctttcaaa cgcgggcctg gtcacgcgcg atgctcgcga
     1141 agtcgagcgc aagaaggtcg gtttccgcaa ggcgcgtcgc cgcaagcagt tctcgaagcg
     1201 ctagggcaag cgcgatcgcg cattcccgcg atcgcgtttc aaccggcgac ggggcgctcc
     1261 ggtaccggcg gctgcctcct cgcggggcag tcatccaaac gggcagggag gccgtcaact
     1321 cggccttccg agggccccgt ttcatgggat gtctgcagcg catttccgcg catcgcggcg
     1381 atgtgcccct cctatacttt ctgcatcaac aacaagaaga gccgccgacc aagcaaggct
     1441 ggcggctctt ttgtttcgcg acgttcactt tgcgtcgccg atggaacact ggccacccgc
     1501 cgatgaagtg gaagttgtgg ttgcgcaatt tgtccgtcag cgcgccccgc gtcgctgtgc
     1561 gcagtcagct gccatgggcg ctgcgcgcaa ttctggcgct gatcttggcg gccctggcgg
     1621 ccgcggctct cggggcgatg tatcactacg cacgcaacgt caggactccg gaccgaagcg
     1681 agatcctcag cgaactcgaa acagcgcgtg agcagtttcg acaggcggcg gcggaacgcg
     1741 aacgcttcgc cgcgcgggcc gtgcaggccg agaatcagct caaggtggaa cgcgtgatgc
     1801 aggagcagat cgccctgcag atgaagacgg tcgaagagga caatgcgcgg ctcaagggcg
     1861 atctggcgtt tttcgaaagt ctgctgccga ctgcgtctgg cgcgcggggc gtggtgatac
     1921 gaagtttcaa gctgcagccc gatgtcgatg acaacaccgt gcgctaccgg cttctggtgc
     1981 agcagagcgg acgacccgaa cgtgacttcg tgggtgccgt ggtgatcaag gtgaacctga
     2041 tgcacgatgg acgcgccgac gtgctgcagc tgcccgatgc cggccaccct gccgcaggcc
     2101 ctgccccatt ggtgtttagg cactaccagc gcctcgaggg cacgttcgcc gtcccagccg
     2161 gcgcgacggt ccggtcggtg caggtgacga ttaacgccgg cggtgagacg cgggcccagc
     2221 agacgttttc gatgtaggga ggcaggatgt ttggacccaa gcgcgcgagc cccaagacga
     2281 tcggcaccct ggtaggggtt ggaacgacca tcgaaggaac gctgtcctac accggtgggc
     2341 tgagaatcga cggtaccgtc aagggcgacg tccggagcgt caacggcgag cagggcatgg
     2401 tgatcatcag cgaacagggc aacgtgatcg gcgaagtcca tgcggcgcac ctcgtggtgt
     2461 ctggcaaggt cgccgggccg ctctttgtca ccgacattct ggaattacag cccaaggcgc
     2521 aggttcaggg tgatgtccac tatcgggcgc tcgaaatcca tcatggcgcc gtggttgaag
     2581 gacgcttgat ccacgacatt cccgacgccc ggccggcctt gaaactggcg gccggtggta
     2641 ccacttccga agctgagtga gtgcaacccc gtgccggggc ctgtgcatat aatggaactg
     2701 acgattttgc gtggagagac ccatgaacgc tgctgttgaa atgcctgccc cgctggtttt
     2761 ttccgatgcg gcagcgtcga aggtgaaggc gctgatcgat gaggaaggaa atccgggcct
     2821 gaagttgcgc gttttcgtcc agggcggcgg ctgctcgggc ttccagtacg gctttacgtt
     2881 cgacgaagtc atcaacgagg atgacgcaac gatggagaaa aacggcgtca tgctcctgat
     2941 cgattcgatg agttaccagt atctggtcgg cgccgagatc gattacaagg aagacctcga
     3001 gggcgcgcag ttcgtcatca agaatccgaa tgcgaccacg acctgtggtt gcggatcgtc
     3061 gttctccgcc tagaccgctt ccgtatctcc gcaaaccaag gggcgccaag tgcgcccctt
     3121 tttgttggcc cttgcagagg ggctgttcaa cgcggataga tcgcgcccag cacccgcgcg
     3181 ccgcgggcgc cggtgaccga cggcaggctt cctgtttcac gccgaataca gcggcgcgcg
     3241 agccacgcaa acgccagcgc ctccacctgg tcaggcggaa tgccgagcgc gtcgctggac
     3301 atgacggagc gcggcgccag taacgcatgc aggtctccca tcagtgcccg attgcgcgtt
     3361 ccgccaccgc ag
//
LOCUS       DATIRT010000237         2476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_724025, whole genome shotgun sequence.
ACCESSION   DATIRT010000237 DATIRT010000000
VERSION     DATIRT010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724025"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(816..2095)
                     /locus_tag="VLN25_05490"
                     /pseudo
     CDS             complement(816..2095)
                     /locus_tag="VLN25_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612169.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2112..>2476)
                     /locus_tag="VLN25_05495"
     CDS             complement(2112..>2476)
                     /locus_tag="VLN25_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10051.1"
                     /translation="GSWLPGEILVNENFRGNLGKTMPTLVHEAAHGVWKADHPRAKGV
                     PEPSDEGGENEYHCVLQELIIYRWMRDTLNLAQPDFELDTRVDRLENGTLKSSIINAH
                     KDELMRNSPAPRFSWTLI"
BASE COUNT          469 a          754 c          800 g          453 t
ORIGIN      
        1 gcccggttcg ctgttcacca actacaccta caatggcttt ggaaagcttc ttacctcgac
       61 catgacgcgg agcggcgtga cacagacgcg cacctgggtt tacgacccgg ccactcagcg
      121 cctcacgtcc atgacgatgc cggaaaccgg caccacgagt ttcacgtaca actcccaagg
      181 tctcgttgcc tctaagacca acgccaaggg ccagcggatc gattacatcc gcgacagcta
      241 caaccggctg acccaggtca agcgcttcaa cagcagcggc gtggagcaga ctgcgcagcg
      301 gaccacctac gagtgggaca cgacggagaa tggctggggg cgcgtgggcc ggatctacac
      361 gaatttcgtg gagtcgtacg actacacgcc cggcggcctg atcaagaaga agacgattgc
      421 cggagaatat gcgctgacgc tggaatacac gtacaacaac gaaggcgtgc gcacttccat
      481 gaaactcccg caggcctacg aacgcgtgaa cggaatcccg cagccattgc cgcaagtgac
      541 gcacgaatat gtgctggatg cctacaatcg cccgatcgga ttgaaggaaa acacctggga
      601 cccgaacacc agcgccacta cgaccacgga cctggtgacg ggcggagtgt tcaatcccgc
      661 aggacagatg accagcgcaa acatcatcgg ctcggccgaa acccgcagtt tcaattcccg
      721 cggccagatg atcggcatga cgggacccgg cgtttcgctc tcatacacct tctcctgttc
      781 cgcgcgcgat ttcttggaca gtaaattcac catagtcatg cagcttgctg gagcgcaagg
      841 agacgctcgc gagcctgccg cggggattgg aaacggagtc gttcgatcag ccactcgcgg
      901 ttgtagcgat tgcggaagtc gcgcagcgcg agctgcagct cttcgaggtc ttggaagtgc
      961 cgcacccaga gaagctgctc tttgagggtg cgaaagaagc gttcaatgca gccgttgccc
     1021 tcgggttcac ggacaaaggc aggagaggac tccataccga gaaagcggag ctccttctga
     1081 aagtggtcgc tcatgtattg tgagccgtgg tcgtggcgga tctgcaaacc gacggcgacc
     1141 ttggggccga aggcgccgaa gtgctcgcga acaccctggc gcaaaggctc cagggcttcg
     1201 aagcgggtgg cgcgcttgac cgcgtgaatg cccacgcact cggcagtgca gtgatcgacg
     1261 gcggcaaaga ccgtgacctg gccttcggcc agagtgacgg tggcggtggc gtcggtgccc
     1321 cacatccggt ttggctgatc ggtgatgatc gatccggcgt gggacttggc ggccaccggc
     1381 gcgagcagcc gctgcggcgc cagcaattgg ttctcgcgca acaggcgcag cacccgaggc
     1441 ttggaagtgc ggacagcctg cacgcgcagt cgcgcccaga tcttgcgatg gccttcgcca
     1501 tgaaacggag aggcggcgag gatggcgcga attttctcca agaggtcggc gtcggtgtag
     1561 ctggtcttcg ggccgcgccg ctgagaggtc accggttgtc tcgcccggtt ctgccgggca
     1621 tagaaggtgg agcgcgccaa tccccagatg gccagcacgc gagcccgccc atacttaccg
     1681 ccggtggagg gcgactgggt ctggctcatc gcttcgacct ccacttcaga gaagggttgg
     1741 cgttctccag ttgctggatg cgctcgcgga gaagctcgtt cgccatcgcc agttcagcga
     1801 tgacggactt catgcgccgg ccgttctcat cgaccagatc gtcttcgcgg atcttcaggc
     1861 cggcctcgcc gctggccaga aaggcgtccc gccaaccgga aagcgtggcg gcagtgaccg
     1921 cgtacttgcg gctcacgctt tccagatcct cgccgcgcag gagttcgagg acgacggcca
     1981 tcttgcgctt ggccgaccag cggcccttgc cgcggcggcc ggtcccgggg gcctccccgg
     2041 tcgcccttcg ggctccctcc gcggcccccg ggaccgcttg cttgctcgtg gacatgctga
     2101 ctctccttct ttcaaatcag tgtccaagaa aatcgtgggg cgggggagtt ccgcatgagt
     2161 tcgtctttgt gggcgttgat tatggaagat ttgagggttc cattttcgag gcggtccacg
     2221 cgggtgtcga gctcgaagtc gggctgggcg aggttgaggg tgtcgcgcat ccagcggtaa
     2281 atgatgagtt cctgaaggac gcagtggtac tcgttctcgc cgccttcgtc ggagggctcg
     2341 ggaacgcctt tcgcgcgcgg gtgatcggcc ttccacacgc cgtgagcggc ttcgtggacg
     2401 agcgtgggca tggtcttgcc gaggttgccg cggaagttct cgttgacgag gatctcgccg
     2461 ggcagccatg atccgc
//
LOCUS       DATIRT010000238         2386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1215594, whole genome shotgun sequence.
ACCESSION   DATIRT010000238 DATIRT010000000
VERSION     DATIRT010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215594"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..971
                     /gene="miaA"
                     /locus_tag="VLN25_05500"
     CDS             63..971
                     /gene="miaA"
                     /locus_tag="VLN25_05500"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HSQ10052.1"
                     /translation="MLLGPTGSGKSALAFDLAQQSPIEIISVDSAQVYRGLDIGTAKP
                     TVAERAAVPHHLIDLRDPAERYSAADFVRDAEIAVAEVRSRGRLPLLVGGTMLYAKAL
                     RDGLSELPSADTSIRAALEEDARRLGWPVMHARLARLDPPTAARLAPNDAQRIERALE
                     IIEATGVSMSSLLAPGRRSSLRLDVVALMPTDRAALHRRIEQRFDEMLRRGFLDEVAS
                     LRQRGDLTADQPSMRSVGYRQAWAHLEGASTYAQFRDAAVAATRQLAKRQLTWLRSLS
                     ADCTIDPLQVDGRGLLGDRLAALGRA"
     gene            1162..1455
                     /locus_tag="VLN25_05505"
     CDS             1162..1455
                     /locus_tag="VLN25_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10053.1"
                     /translation="MKSDDRHARKPMRRVQLIVALVIAPALSALVGHNIGQHATAGDG
                     ALQMQFSNRTGESVFAGRPPSAAPDHALLSATDVERSLPQPARAPAMQESEIL"
     gene            complement(1479..>2386)
                     /locus_tag="VLN25_05510"
     CDS             complement(1479..>2386)
                     /locus_tag="VLN25_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HSQ10054.1"
                     /translation="LGTKALVADAMRAITGRTYYNQIAQDTLAMALNDIITVGATPVS
                     VHAYWATGGSDWFADAARARDLVEGWRAACDRSGVAWGGGETPALAGVVESGRIDLAA
                     SCVGLVRPKSRLTLGERLGDGDAIVLLASSGIHANGVSLARKLAERLPDGYRTRLSDG
                     TMYGEALLVPTVLYSPVTEALAAAGIVPHYSANVTGHGWRKLMRHPGDFAYRLHSLPT
                     IPPVLEFIRRQADISDREAYGNLNMGAGFALYVAAQDAERCVEIAAACGVTAWHAGTV
                     ESGAKQVVIEPLQLAFAADDLHVRA"
BASE COUNT          384 a          858 c          768 g          376 t
ORIGIN      
        1 cttcgcgagt catcgcggca aaccgctcga cccggcccga ggtccccatt gccgacgcgc
       61 tgatgctgct cggaccgacc ggcagcggca agagcgcgct cgcattcgac ctcgcacaac
      121 agtctccgat cgagatcatc agtgtcgact cggcgcaggt gtaccgcggc ctcgacatcg
      181 gcaccgccaa gcccaccgtc gccgagcgcg ctgccgtccc ccaccacctg atcgatctgc
      241 gtgatcccgc ggagcggtat tcggccgccg acttcgtccg ggacgccgag atcgccgttg
      301 ccgaggttcg gtcgcgaggc aggctaccgc tgctggtagg cgggacgatg ctgtacgcga
      361 aggccctgcg cgacggcctg tccgaactgc cctcggccga tacttcgatc cgcgccgcgc
      421 ttgaagagga cgcgcgccgc ctaggatggc cggtcatgca cgcgcggctg gcccggctcg
      481 atccgcccac cgcggctcga ctggccccca acgatgcgca gcgcatcgag cgcgcactgg
      541 agatcatcga agcgaccggc gtttcgatgt cgagcctgct cgcaccgggc cgccgttcat
      601 cgctgcggct cgatgtcgtc gcgctgatgc ccaccgatcg cgccgcgctg cacaggcgca
      661 tcgaacagcg cttcgacgag atgctcaggc gaggcttcct cgacgaggtc gccagcttgc
      721 gacaacgcgg cgacctgacc gccgaccagc cgtcgatgcg cagcgtcggt taccgccagg
      781 cctgggctca cctcgaggga gccagcacgt acgcacagtt ccgtgacgcc gccgtcgccg
      841 cgacgcgcca gctcgctaag cggcagctga cctggctgcg ttcactgtct gccgattgca
      901 cgatcgatcc gctgcaggtc gacggacgcg gactgctagg agatcgcctc gcggcacttg
      961 ggcgggcata gcatcggtcg ctcgccccag cggcgcggcc tcagctcatg catgccgccc
     1021 ttgcccgttt gtgaattttc gacagtgtca gcacggctca atccggtctg cacacccgga
     1081 ctttcgcgct gtacgcgcgc accgtcccgc gcacactggc gtcgtgctgc acatgaaacg
     1141 atgcagcgtg cggaggtcag tatgaaatca gatgatcgtc atgccaggaa gccaatgcgg
     1201 cgcgtgcaac tcattgtcgc gctggtcatc gcaccggcct tgagtgcgct ggtcggacac
     1261 aacatcgggc agcacgcgac ggctggggat ggcgcgcttc agatgcagtt ctcgaaccgg
     1321 actggtgaat cggtcttcgc cggcaggcca ccgtcggccg cacccgacca tgcgctgctg
     1381 tcggccaccg acgtcgagcg ctcactgccg cagcctgcgc gcgccccggc gatgcaggag
     1441 tcggagatcc tgtagcgaaa cgcccggcgg ccgcttcctc aggcgcgtac gtgcagatcg
     1501 tcggcggcga aggcgagctg gaggggttcg atcacgacct gcttcgcacc cgactccact
     1561 gtcccggcat gccaggccgt cacaccgcac gcagcagcga tctcgacaca gcgctccgcg
     1621 tcctgcgcag cgacgtacaa cgcgaagccc gcgcccatgt tgagattgcc gtaggcctcg
     1681 cggtcgctga tgtccgcctg ccgacggatg aattcgagca cggggggaat cgtcggcagc
     1741 gagtgcaggc gatacgcgaa atcgccggga tggcgcatca gcttgcgcca gccgtgtccc
     1801 gtgacattcg cgctgtagtg cggaacgatc ccggcggccg ccagcgcttc ggtcaccggt
     1861 gaatacagca cggtcgggac cagcagcgcc tcgccgtaca tcgttccgtc ggacaggcgt
     1921 gtccggtagc catcgggaag tcgctcggcc agcttgcgtg ccagcgaaac gccattggcg
     1981 tgtatgccgc tcgatgccag caggacgatc gcatcaccgt cacccagccg ttcacccagc
     2041 gtcaagcgcg acttcgggcg gaccagtccg acgcacgacg cggccaggtc gatgcggccg
     2101 gactcgacca ccccggccaa tgcgggcgtt tccccgccgc cccaggccac gccgctgcga
     2161 tcgcaggctg cgcgccatcc ctccaccaga tcgcgcgcgc gcgccgcgtc agcgaaccag
     2221 tcgctgcccc ccgtcgccca ataggcatgt accgacactg gcgtcgcgcc gacggtgatg
     2281 atatcgttaa gcgccatcgc cagcgtgtcc tgcgcgatct ggttgtagta cgtgcggccg
     2341 gtgatcgccc gcatcgcgtc ggcgaccagc gccttggtgc cgaggc
//
LOCUS       DATIRT010000239         8923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1805894, whole genome shotgun sequence.
ACCESSION   DATIRT010000239 DATIRT010000000
VERSION     DATIRT010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8923
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1805894"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VLN25_05515"
     CDS             complement(<1..274)
                     /locus_tag="VLN25_05515"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family cytochrome oxidase biogenesis
                     protein"
                     /protein_id="HSQ10055.1"
                     /translation="MDALITPAAARRHVYLAICLGVVVTIVGVLLGQWQTRRGDMKEA
                     LQARWDAAATAMPMQVKSVDDAAAVAAGLPRRVELRGEFLPAATVFV"
     gene            308..505
                     /locus_tag="VLN25_05520"
     CDS             308..505
                     /locus_tag="VLN25_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HSQ10056.1"
                     /translation="MRIIIVIAFIGIIVSLGSALVFLVRDHGNTNRTVNALTVRVGLS
                     VALFLFILFSYWMGWIEPRSY"
     gene            complement(578..1441)
                     /locus_tag="VLN25_05525"
     CDS             complement(578..1441)
                     /locus_tag="VLN25_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HSQ10057.1"
                     /translation="MANTHGGAAPDYFVPAPSQWPVVGMIAMAFTGFGAAMVVNHSST
                     GWVPLAIGMATLVYMMVGWFSMVSSESEHGAYNRKVDASFRWGMGWFIFSEVMFFASF
                     FGALFYARVITAPMLGDFDHKLLWPDFDPAWPHAGPAGVVDPFTAMGPFWLPTINTAL
                     LLTSGVTLTIAHHALRVNHRNTAASWLALTVLLGVTFLGVQIYEYSHAYSQLNLKLSS
                     GIFGSTFFMLTGFHGFHVFVGAMMLGVVLLRLLRGHFKPDHHFAFEGAAWYWHFVDVV
                     WIGLYILVYWL"
     gene            complement(1464..1694)
                     /locus_tag="VLN25_05530"
     CDS             complement(1464..1694)
                     /locus_tag="VLN25_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2970 domain-containing protein"
                     /protein_id="HSQ10058.1"
                     /translation="MPDTGGSSPEPATGARNAGLLQTLRAVLWSFFGVRKRSGYEQDA
                     SQLNPVVVIIAGVIAAAIFVLTLLVIVRIVVS"
     gene            complement(1698..2291)
                     /locus_tag="VLN25_05535"
     CDS             complement(1698..2291)
                     /locus_tag="VLN25_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HSQ10059.1"
                     /translation="MSERPQVSEIQSDNRTILRKLVIVSVLMFGFGWALVPLYRKICE
                     ITGINVVTLRDANAERAARNSQVDLSRTITVEFDANTQGAWRFRPHVNHLQVHPGELV
                     QVTYDLVNLEGRAMAGQAIPSYAPMQSASYFQKLECFCFQQQTLAAGETRQFPVVFFI
                     DPDLPQDVKTITLSYTFFEVAGAVAKPQAVRATSPSG"
     gene            complement(2306..2422)
                     /locus_tag="VLN25_05540"
     CDS             complement(2306..2422)
                     /locus_tag="VLN25_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome oxidase small assembly protein"
                     /protein_id="HSQ10060.1"
                     /translation="MSPESDERRHNRRTALILASVVLVFFIGVIARRWLVGG"
     gene            complement(2437..4035)
                     /gene="ctaD"
                     /locus_tag="VLN25_05545"
     CDS             complement(2437..4035)
                     /gene="ctaD"
                     /locus_tag="VLN25_05545"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HSQ10061.1"
                     /translation="MSAVVHTPTAGHGDHDHAHDHPHGWRRWLYATNHKDIGTMYLWF
                     SFTMFIIGGVLALGIRLELFQPGLQYWRPEFFNQLTTMHGVIMVFGAIMPAFVGFANW
                     MVPLQIGAPDMAFARMNNFSFWLLPPAALLVVASFFVPGGATAAGWTLYAPLTVQMGP
                     GMDMAIFALHILGASSIMGSINIITTILNMRAPGLTLMRMQMFVWTWLITAYLLIAVM
                     PVLAGAITMTLTDRHFGTSFFNAAGGGDPVMYQHIFWFFGHPEVYIMILPAFGIVSQI
                     IPVFARKPLFGYASMVYATASIAILSFIVWAHHMFTTGMPVTGQLFFMYATMLIAIPT
                     GVKVFNWVATMYKGSMTFETPMLFAVGFIFVFTMGGFTGLILAMAPIDIQLQDSYYVV
                     AHFHYVLVAGSLFALFAGTYYWLPKWTGHMYNETLGKWHFWLSMIFFNITFFPMHFLG
                     LAGMPRRYADYPLQFADFNLLASIAAFGFGFTQLLFLYIVVKCIKGGPKAAANPWGAD
                     DGGLEWTVPSPAPFHTFETPPVVR"
     gene            complement(4072..5148)
                     /gene="coxB"
                     /locus_tag="VLN25_05550"
     CDS             complement(4072..5148)
                     /gene="coxB"
                     /locus_tag="VLN25_05550"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HSQ10062.1"
                     /translation="MAGAAGSAAAIQSRFNLAPPVTPVAHEIYSLHNLMLVICTVIFV
                     AVFSVMFYSVWKHRKSRGAVAANWHENATVEIIWTVIPFLIVIVMALPATRTVVAMKD
                     TSNSDLTIKVTGYQWKWGYDYLRGEGEGISFLSQLSTPVAQIEGREPRSSTYLIEVDN
                     PLVVPVNKKVRVITTANDVIHAWWVPAFAVKQDAIPGFARDTWFRAEQIGTFRGQCAE
                     LCGKDHAFMPIVVQVVSAEDYSKWVSGKKQQMAAAADDPTKQWTAAELIARGEKVYQA
                     NCQACHQANGLGVQGAFPTLVGSKVVASTGDQITMLLNGKNAMPSWKSLSDVELAAVA
                     TYTKNAWGNKAGEVQPAEFKSARK"
     gene            complement(5198..5707)
                     /locus_tag="VLN25_05555"
     CDS             complement(5198..5707)
                     /locus_tag="VLN25_05555"
                     /inference="COORDINATES: protein motif:HMM:NF021517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HSQ10063.1"
                     /translation="MTVSKGGLIKRNCSAGPRQLALVFASLMAVSFTFGVAFAAQGPW
                     MVLPFVGLEIVAVAAVFLAYGRHAADFECIELRDGWLIVEKHVGSRRSEFVFDLPWVW
                     VDMQKRGVDLGTGVRVERVPAQQRVEIGGYLANVGRSAWARELGRTRASGLTGHVIDS
                     KPGKRTGNG"
     gene            complement(5894..6388)
                     /gene="lysM"
                     /locus_tag="VLN25_05560"
     CDS             complement(5894..6388)
                     /gene="lysM"
                     /locus_tag="VLN25_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HSQ10064.1"
                     /translation="MSLISFFKEAGEKLFGKKVQEVQAAPAQVDAAALAEANREAATA
                     IEKYINTLGLTVTALTVTFDGATSTAKVFGVAKDQTTKEKVILAAGNVYGVAAVQDMM
                     TVDLSQPEAKFYTVVKGDTLSKIAKEFYGNANAYTKIFEANKPMLSHPDKIYPGQNLR
                     IPAE"
     gene            complement(6467..7408)
                     /locus_tag="VLN25_05565"
     CDS             complement(6467..7408)
                     /locus_tag="VLN25_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSQ10065.1"
                     /translation="MPLGPGTSELPPTIVAPPRVPACTETVARQFDQRVGRIAARDLL
                     LREVEQRLLSRLEYIRLDPRQILDVGCGLGRSRAGLHSLYPQATWTGIELSAAMARVG
                     LAEQQRALGVSRWWSSAPRWVVADGARLPSADSTVDLLFSNLMLHWHPTPHLVFPEWK
                     RVLRTNGLLMFSCFGPDTLRELRAAAADALPHSRPMPFVDMHDFGDMLVASGFATPVM
                     DVETLTLTFSSPRDLIAEVRSLGANPRSDRWPALASGRQARRLLEALAARRDAAGRIP
                     LSFEIAYGHAWKPPPRTSGSSVITVDALRQHLSEIRK"
     gene            7867..8301
                     /locus_tag="VLN25_05570"
     CDS             7867..8301
                     /locus_tag="VLN25_05570"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HSQ10066.1"
                     /translation="MVAALKFGARLDLAEAFARLLAAREPRPVADLIVAVPLSFERES
                     ARGFNQSLEIARRYARLTGATMAERTLLRVRHAPPQQSLAREARRRNVRGAFGVSGDV
                     RGARLTVVDDVMTTGSTLNEIADTLKRAGAARVVNRVVARTP"
     gene            8317..8787
                     /locus_tag="VLN25_05575"
     CDS             8317..8787
                     /locus_tag="VLN25_05575"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HSQ10067.1"
                     /translation="MFNVVLVEPEIPPNTGNVIRLCANAGARLHLVEPLGFDLSDKQL
                     RRAGLDYHEYADMRVHASFEALLAQEHPVATRMFCCTTHAERHHADVAFEPGDWLVFG
                     AESRGLDESLRARFPPTQRLRLPMRAGNRSINLSNAVAIVVYEAWRQQRFEGGA"
BASE COUNT         1685 a         2922 c         2765 g         1551 t
ORIGIN      
        1 cgacaaagac cgtggcagcg ggcaggaatt ccccgcgcaa ctcgacccgc cgcggcaatc
       61 ctgcagcgac cgcggccgca tcatccaccg acttcacttg catcggcatc gcggtggcgg
      121 ccgcatccca gcgcgcctgc aacgcttcct tcatgtcgcc gcggcgcgtc tgccattggc
      181 ccagcagcac ccccacgatc gtgaccacca cgccgaggca gatggcgaga tagacgtgac
      241 gacgggccgc tgccggggtg atcagggcgt ccaatgggat aatccaccag ccgcgccgcc
      301 ggcctttatg cgcatcatca tcgtcatcgc cttcatcggc atcatcgttt cactgggctc
      361 tgctctggtc ttcctggtgc gcgaccatgg caacacgaat cgaacggtca atgccctgac
      421 cgtccgggtc gggctgtcgg ttgcgctctt tctgtttatc ctgttcagct attggatggg
      481 ctggatcgag ccgcgttcct actagggaac gttgcgggca gaaagaactg cggcgcccgc
      541 aggcgccgcg atgtgaaggt cacgcggccg gcgacggcta cagccagtag accagaatgt
      601 aaaggccgat ccacacgacg tcgacgaagt gccagtacca ggccgcccct tcgaatgcga
      661 agtgatggtc cggcttgaag tgcccgcgca gcaggcgcag gagcacgaca cccagcatca
      721 ttgcaccgac aaagacgtga aagccgtgga acccggtcag cataaagaac gtcgagccga
      781 agatgccgga cgacagcttc aggtttagtt gcgaataggc gtggctgtac tcatagatct
      841 gaacgccgag gaaggtcacg ccgagcagca cggtgagcgc cagccacgat gccgccgtgt
      901 tccgatgatt aacccgcaac gcatggtgcg cgatcgtgag cgtcacgccg gaagtcagca
      961 gcagcgccgt gttgatcgtc ggcagccaga acggtcccat cgcggtgaat ggatcgacga
     1021 cgccggcagg tcccgcgtgc ggccatgccg gatcgaagtc cggccacagc agcttgtgat
     1081 cgaaatcccc gagcatcggc gcggtgatga cccgcgcgta gaacagcgcg ccgaaaaacg
     1141 acgcgaaaaa catgacttcg gagaagatga accatcccat tccccaacgg aatgacgcat
     1201 cgaccttgcg gttgtaggcg ccatgctccg actcgctgga gaccatcgag aaccatccga
     1261 ccatcatgta gacgagcgtg gccattccga tcgccagcgg cacccagccg gtcgagctgt
     1321 ggttgaccac catcgccgcg ccaaagccgg taaaggccat cgcgatcatg ccgaccaccg
     1381 gccattgcga aggcgccggt acaaagtagt ccggcgccgc tcccccatgc gtattggcca
     1441 tttttcctgt tccccttctt ttcctagctc accacgatcc gaacgatcac cagcagcgtc
     1501 agaacaaaga tcgcggccgc aatcacgccg gcaatgatca cgaccaccgg gttgagctgt
     1561 gacgcgtcct gctcataacc ggaccgcttg cgtacaccga agaacgacca cagcaccgca
     1621 cggagcgtct gcagtaagcc cgcgttccgc gcccccgtag cgggctctgg cgacgaccca
     1681 ccggtgtccg gcatcagtca gccactcggc gaggttgcgc ggaccgcctg cggcttggca
     1741 accgcacccg ccacttcaaa gaatgtgtag gacaacgtga tcgttttcac atcctgcggc
     1801 aggtcaggat cgatgaagaa cacgacaggg aattggcgtg tctcccccgc cgccagcgtc
     1861 tgttgctgaa agcagaaaca ttcgagcttc tggaaataag atgccgactg catcggcgcg
     1921 tagctaggta tggcctggcc ggccatcgca cggccttcga gattgaccag gtcataggtc
     1981 acctgaacca gttcacccgg atgcacctgt agatgattca catgcggccg gaaccgccac
     2041 gcaccctgcg tgttggcgtc gaactccacg gtgatcgtgc gcgaaaggtc gacctgcgaa
     2101 tttcgcgcgg ccctttccgc attggcatcg cgcagggtga ccacgttgat accggtgatc
     2161 tcgcagattt tgcgatacag cggcaccagc gcccagccga atccgaacat cagcaccgac
     2221 acgatgacca gtttgcgcag gatcgtgcgg ttgtcgctct ggatttctga aacctgggga
     2281 cgctcgctca tggtcagtcc cgccctcagc cgccgacaag ccaacgacgc gcgatgacgc
     2341 cgatgaaaaa caccagcacg accgacgcga gtatcagtgc cgtgcggcgg ttgtgcctgc
     2401 gttcgtcgga ctcggggctc atcgtgtgca ccctgcttac cgcacgaccg gaggcgtctc
     2461 gaacgtgtgg aacggtgctg gcgacggcac ggtccactcg aggccgccgt cgtcggcgcc
     2521 ccacgggttc gccgccgcct tcggcccacc cttgatgcac ttgaccacga tgtacaggaa
     2581 cagcagttgc gtgaagccga agccgaatgc ggcgatcgaa gccagcaggt tgaaatcggc
     2641 aaactgcagc ggatagtcgg cgtagcggcg cggcatgccg gccagcccga ggaagtgcat
     2701 cgggaagaac gtgatgttga agaaaatcat cgacagccag aagtgccact tgccgagagt
     2761 ttcgttgtac atgtgcccgg tccacttcgg cagccagtag taggtgccgg cgaagagcgc
     2821 aaacagtgaa cctgccacca gcacatagtg gaagtgcgcg accacgtagt aggaatcctg
     2881 cagctggatg tcgatcggcg ccatcgccag gatcagcccc gtgaatccgc ccatcgtgaa
     2941 cacgaagatg aagccgaccg cgaacagcat cggcgtctcg aaggtcatcg agcccttgta
     3001 catcgtcgcg acccagttga agaccttcac gccggtcggg atggcgatca gcatcgtcgc
     3061 gtacatgaag aagagctggc cggtgaccgg catgccggtg gtgaacatgt ggtgcgccca
     3121 gacgatgaac gacaggatcg cgatcgacgc ggtcgcgtag accatcgacg catagccgaa
     3181 cagcggcttg cgcgcgaaca ccgggatgat ctggctgacg ataccgaagg ccggcaggat
     3241 catgatgtag acctcgggat gcccgaagaa ccagaagatg tgctggtaca tcacggggtc
     3301 accgcccccg gcggcgttga agaagctggt gccgaagtgg cgatccgtca gcgtcatcgt
     3361 gatggcgccg gcgagcaccg gcatcaccgc aatcagcagg taggccgtga tgagccacgt
     3421 ccacacgaac atctgcatcc tcatcagcgt gagaccgggg gcgcgcatgt tcaggatggt
     3481 cgtgatgatg ttgatcgaac ccatgatcga cgacgcgccg aggatgtgca gcgcgaagat
     3541 cgccatgtcc atgcccgggc ccatctgcac ggtcagcggt gcatacagcg tccagccggc
     3601 ggctgttgca ccgcccggca cgaagaatga ggcaacgacc agcagtgccg cgggcggcag
     3661 cagccagaag ctgaagttgt tcatgcgcgc gaaggccatg tcaggcgcgc cgatctgcaa
     3721 cggcaccatc cagttcgcga agccgacgaa ggccggcatg atcgcgccga acaccatgat
     3781 gacgccgtgc atcgtcgtca gctggttgaa gaattccggg cgccagtact gcaggccggg
     3841 ctggaacagc tcaagccgga tcccaagggc cagcacgcca ccgatgatga acatcgtgaa
     3901 gctgaaccac aggtacatcg ttccgatgtc cttgtgattc gtggcataca gccaccggcg
     3961 ccagccgtgc ggatggtcat gggcgtgatc gtggtcgccg tgtccggcgg tcggggtgtg
     4021 gacaactgca ctcatcggta ctcctgatcc tgatcgggtg tgctgcgacg tctacttgcg
     4081 cgcggacttg aattcggcgg gctgaacttc acccgccttg ttgccccacg cgttcttcgt
     4141 gtaggtcgcc accgccgcca actcgacgtc ggaaaggctc ttccacgacg gcatcgcatt
     4201 cttgccgttc agcagcatcg tgatctgatc gccggtgctg gcaaccacct tgctgcccac
     4261 caacgtcggg aacgcaccct gcacgccaag cccattcgcc tggtgacagg cctggcagtt
     4321 cgcctggtag accttttcac cgcgcgcgat cagttcggcg gcagtccact gcttggtcgg
     4381 gtcatcggcc gccgcagcca tttgctgctt cttgccgctg acccacttgc tgtagtcctc
     4441 ggccgacacc acctgcacga cgatcggcat gaacgcgtgg tccttgccgc acagttcggc
     4501 gcactgaccg cggaacgtgc cgatctgctc ggcgcggaac caggtgtcgc gcgcaaagcc
     4561 cgggatcgcg tcctgcttga ccgcaaaggc gggcacccac catgcatgga tcacgtcgtt
     4621 ggcggtcgtg atcacacgca ccttcttgtt gaccggaacg acgagcgggt tgtcgacctc
     4681 gatcagatag gtgctggacc gcggctcacg cccttcgatc tgtgcgaccg gcgtggacag
     4741 ctgcgacagg aacgagatgc cttcgccttc gccgcgcaga tagtcgtagc cccacttcca
     4801 ctggtatccg gtgaccttga tcgtgaggtc cgagttgctc gtgtccttca tcgcgacgac
     4861 agttctcgtc gccggcagcg ccatgacgat cacgatgagg aacgggatga cggtccagat
     4921 gatctcgacg gtggcgttct catgccagtt ggcggccacg gcaccccggg acttgcggtg
     4981 cttccagacg gaatagaaca tcacgctgaa caccgccacg aagatgacgg tgcagatgac
     5041 cagcatcagg ttgtgcaggc tgtatatctc atgagcgacc ggagtgaccg gcggcgccag
     5101 gttgaaccgg ctctggatcg cggcagcgct gcccgctgcg ccggccatca ggatcgccag
     5161 caccgcgcgc cagactgcaa atcgattgaa cactgtctta gccattccct gtccgttttc
     5221 ccggcttcga atcaatcaca tgccccgtta agccgctcgc ccgagtgcgg cccagttctc
     5281 gcgcccaagc gctgcgcccg acattcgcga ggtacccccc gatttcgacg cgctgctgcg
     5341 ccggcacccg ttccacccgc acgcctgtgc cgaggtcgac accgcgtttc tgcatgtcga
     5401 cccacaccca gggcaggtcg aatacgaact ccgaacgacg cgacccgacg tgtttctcca
     5461 cgatgagcca gccgtcccgc aattcgatgc actcgaagtc cgccgcgtgc cgtccgtagg
     5521 ccaggaagac cgcagcgacg gccacgattt caagacccac gaacggaagc accatccatg
     5581 ggccctgcgc ggcgaaggcc accccgaagg tgaacgagac agccatcagt gaagcgaaca
     5641 ccagcgccaa ctgccgcggg cccgctgaac agttacgctt gatcaaccca cccttggaca
     5701 cggtcataca cccgcctgcc ctcgcgtcga ccggggtcgg cgcaaaacgg gcggattata
     5761 tgcaaagcga cgcgtcaggt cggcgcgacc gccggcttgc acccaggcac cgtccgatca
     5821 gcgggatggc acgcgcccga cgctgcgccc gggccggccg gacggacccg gctcccgccg
     5881 ggccggcacg cgattactcg gcgggaattc gcaggttctg ccccggatag atcttgtccg
     5941 gatggctcag catcggcttg ttcgcttcga agatcttcgt gtaggcattg gcgttgccgt
     6001 agaactcctt ggcgatcttc gacagcgtgt cgcccttgac gaccgtgtag aacttggcct
     6061 cgggctgcga caggtcgacc gtcatcatgt cctgcacggc tgctacacca tagacgttgc
     6121 cggccgccag gatcactttc tccttcgtag tctggtcctt ggcgacgcca aacaccttgg
     6181 ccgtgctggt cgccccgtcg aaggtgaccg tcagcgcggt caccgtgagg cccagcgtgt
     6241 tgatgtattt ctcgatcgcg gtggccgctt cgcggttcgc ttcagcgagc gcggccgcgt
     6301 cgacctgcgc cggtgcagcc tggacttcct ggaccttctt gccgaacaac ttctctccag
     6361 cttccttgaa aaacgaaatg agactcatct tttctccact agttgttccc cgcagcgggg
     6421 acgatccttc gacgcgcggc tcatttgccg ctcacatagt cgcactttac ttccttattt
     6481 ctgacaggtg ctgacgcagg gcatccaccg tgatgacgct gctcccggaa gttcgaggtg
     6541 gcggcttcca cgcgtgcccg tacgcgattt cgaagctcag agggatgcgg ccggccgcgt
     6601 cgcggcgagc cgcgagtgcc tccagcaagc gccgcgcctg tcggccagac gccaacgccg
     6661 gccagcggtc gctgcgcggg ttggcgccga gcgaccggac ttccgcgatc aggtcgcgcg
     6721 gactactgaa tgtgagggtc agcgtttcca cgtccatgac gggcgttgcg aagccgctgg
     6781 ccaccagcat gtcaccgaag tcgtgcatgt cgacgaaggg catcggccgg gaatgcggca
     6841 gggcgtcggc agccgccgcc ctcaattccc gcagcgtgtc cgggccgaag cagctgaaca
     6901 tcagcagtcc gttggtccgc agcacgcgct tccactccgg gaaaaccaga tgcggcgtcg
     6961 gatgccaatg cagcatgagg ttcgaaaaca gcaggtccac ggtgctgtcc gcggacggca
     7021 agcgcgcgcc gtcggccaca acccagcgcg gcgcgctgct ccaccagcgg gacacgccga
     7081 gcgcgcgctg ctgctcggcg agaccgacgc gcgccatcgc ggccgacaac tcgataccgg
     7141 tccatgtcgc ctgcggatat agggagtgca ggcctgcgcg tgagcgtccc agtccgcaac
     7201 cgacatccag tatctggcgg ggatcgagcc tgatgtactc gagccggctc agcaaccgct
     7261 gctcgacttc gcgcaagagc agatcgcgcg ccgcaatccg tccgacgcgc tgatcgaact
     7321 gccgtgccac cgtttcggtg catgccggca cgcgcggcgg cgccacgatc gtgggcggaa
     7381 gttcggatgt tccagggccg agcggcatga tgcgggccag tataggttga cgctagagcc
     7441 gttcacgcgt caaacgttca cgcttcaaac actcaagcgc aagactcccg gccgcgcatt
     7501 cggcggcgaa agcctcgagt gcgagctcgc aggaagccgt cgcaaccgcc ttcggcacag
     7561 gtcgctcagg cgtctgcgga tcgctaagct tcgccaatgc agtcaaggtc atctcagcat
     7621 ctcggcgccg gccccaggcg ggccggctgg attgcgccgc gttgcgtcgc ctgtgccctg
     7681 ttcccgggtt cgccgctgtg tgagggctgc gagcgggatt tcttcgcacc cgaggcggcc
     7741 cggtgcgaac gctgcgcaat cagactcggc aacgcatcgg cgggctgctg cggacattgc
     7801 ctggcggagc ccccgcattt cgatgcgacc accactctgg ccgactacgt tgcgcctgtc
     7861 tcggggatgg tcgccgcgtt gaagttcggt gcaagactcg atctcgccga agcgtttgct
     7921 cgtctgctgg ccgcccgcga gccccggcct gtggccgatc tcatcgtggc agtgccgttg
     7981 tcgttcgaac gcgagagtgc acgcggcttc aaccagtcac tggaaatcgc acggcgctac
     8041 gcccggctca ccggcgccac gatggccgag cgcacgctgc tcagggtgcg acatgcgccg
     8101 ccgcagcagt cactcgctcg cgaggctcgt cgacgcaatg tccgtggcgc gttcggcgtc
     8161 tccggagacg tgcggggcgc ccgactcacc gtggtcgacg atgtgatgac cactggcagc
     8221 acgctgaacg agattgctga caccctgaag cgcgccggcg ctgcgcgtgt cgtcaatcgg
     8281 gtcgtcgcac ggactccctg agttggcagc tgcccgatgt tcaacgtcgt actggttgag
     8341 ccggaaattc ccccgaacac cggcaatgtc attcgtctgt gcgccaatgc cggtgcacgc
     8401 ctgcatctgg tcgagccgct cggattcgac ctgtcggaca agcaattgcg ccgtgcgggg
     8461 ctcgactatc acgaatacgc cgacatgcgc gtgcatgcga gcttcgaagc actgctcgcg
     8521 caggagcatc ccgtggccac gcgcatgttc tgctgcacga cgcacgcaga acgacatcac
     8581 gccgacgtgg cgttcgagcc cggcgactgg ctggtgttcg gagccgagtc gcgcggcctc
     8641 gatgagtcac tgcgcgctcg attccctccg acccagcgcc tgcgcctgcc gatgagagcc
     8701 ggcaaccgca gcatcaacct gtcgaatgcc gtcgcgatcg tcgtctacga agcctggcga
     8761 cagcagcgat ttgaaggcgg cgcctgagaa ggcagttcgg ggaggcgccc gaggctggcg
     8821 ccgatcagct gcgctgggac tcgactttcg gtgggtggct gcggctcatc tggatcgcgt
     8881 gcaggtccag acggtcgagc aggagttccg gcgtgtgatc gac
//
LOCUS       DATIRT010000240         4367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_35343, whole genome shotgun sequence.
ACCESSION   DATIRT010000240 DATIRT010000000
VERSION     DATIRT010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35343"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VLN25_05580"
     CDS             complement(<1..358)
                     /locus_tag="VLN25_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HSQ10068.1"
                     /translation="MGTLTALALIVAMFVILGAGVWIGIAVLGVAWLGMELFTTRPGG
                     DALALTVWGSLSSWTLTALPLFIWMGEILLKSRLSEGMFRGLAPWLQWLPGRLLHTNI
                     IGCTIFAAVSGSSAATC"
     gene            complement(365..874)
                     /locus_tag="VLN25_05585"
     CDS             complement(365..874)
                     /locus_tag="VLN25_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HSQ10069.1"
                     /translation="MRSFLDPLFKLLGGLGAFFIFATLAIEVVNIAGRHLGFSMAGMD
                     AYAGYFLAAGSFLAMGYALRQGDHIRVTLILQRLHGRARWWMEVLCLVIATLLSGYFA
                     WFAARLVWGSFAFNDISQNVDATPLWIPQLSMAIGLAGLALAFVEQLVYVLRFRTLPP
                     QPNEAANIE"
     gene            complement(924..1901)
                     /locus_tag="VLN25_05590"
     CDS             complement(924..1901)
                     /locus_tag="VLN25_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HSQ10070.1"
                     /translation="MNRRLPLLGASAIASFLLALPVSAQTKWDLPAGYPAGNPHTQNL
                     NQFAADVAKATGGKLNITVHAGGALFKAPEIKRAVQTGQAQVGEVLMSLLENENPVFG
                     ADAVPFVATGFDASWKLWQAQRPVVEKILAAQGVKVLYAVAWPPQGIYTKKQLNSVAD
                     MKGLKWRAYNPATTRIAAMVGAQPLTIQAAELSQALATGVVDSFMSSGATGVDSKVWE
                     SLSHFATADAWLPKNMVIVNQKEFDKLDKATQDAVLAAAMAAEERGWKRMREYTGESL
                     ATLKANKMTVYEPNAAFKAELNKLGETLLTEWLAKAGPDGQAIITQFRK"
     gene            2011..>4367
                     /locus_tag="VLN25_05595"
     CDS             2011..>4367
                     /locus_tag="VLN25_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HSQ10071.1"
                     /translation="MAHEQWEFWIDRGGTFTDIVARRPNGLLATLKLLSENPEQYRDA
                     AVAGIRQLLCVAAEAPIPFEQIAAVKMGTTVATNALLERKGEPTALVISRGFRDALRI
                     AYQNRPRLFERHIVLPELLYARVIEADERVGAHGDILQPLDETALASELRAAYAAGLR
                     SCAIVLMHGYRYTAHEQAAARLAREAGFPQVSVSHEVSPLMKLVARGDTTVVDAYLSP
                     ILRRYVEQVATDLPGARLLFMQSNGGLTDAWRFQGKDAILSGPAGGIVGMARASRHAG
                     FDRVIGFDMGGTSTDVSHYAGEYEREFETLVAGVRMRAPMMSIHTVAAGGGSILHFDG
                     ARLRVGPDSAGANPGPACYRRGGPLAVTDANVMLGRVQPGYFPPVFGPSGLEPLDRDV
                     VVRHFSDLAREIESATGRTQTPEQVAGGFVEIAVGNMAEAIKRISVQRGHDVTRYTLT
                     VFGGAGGQHACAVADALAMTTVFAHPLAGVLSAYGMGLADQTAMREMTIEQTLVAETL
                     PHLEASLSTLASQAMASLQSQGVARERIGLVRRVHVKYEGTDTALAVGYGTIDAMRAQ
                     FDAAYQQRFSFLMPDRRLIAETVSVEAIGGGDASTSLGSAQRPARAGPLKASATVQAF
                     FDNAWHATPLYRRDDTQPGDHIDGPAIVAEANATTVIEPGWQAEVTPLDHLVLHRIVP
                     RAARRAIGTTVDPVMLEIFNNLFMSIAEQMGLRLQNTAYSVNIKERLDFSCALFDATG
                     NLIANAPHMPVHLGSMSESIKTVMRENAGSMVPGDAFVINDPYHGG"
BASE COUNT          752 a         1429 c         1487 g          699 t
ORIGIN      
        1 cacaggtggc ggccgacgag cccgacacgg cggcgaagat cgtgcagccg atgatgttcg
       61 tatgcagcag tcgccccggc agccactgca gccagggcgc aaggccgcgg aacatgcctt
      121 cggacaggcg cgacttcagc aagatctcgc ccatccagat gaaaagcggc agcgcggtca
      181 gggtccagct cgacagcgat ccccacacgg tcagagcaag tgcgtcgccg cccggtcgcg
      241 tcgtgaaaag ttccatgccg agccatgcga cgccgagcac ggcgatgccg atccagacgc
      301 cggcgcccag gatcacgaac atcgcaacga tcagtgccag tgcggtcagt gtccccatcg
      361 cggcctattc gatgttggcg gcttcgttgg gctgtggtgg cagcgtgcgg aagcgcagca
      421 cgtagaccag ctgctcgacg aacgcgagcg cgaggccagc caggccaatg gccatcgaca
      481 gctgcgggat ccacaacggg gtcgcatcga cgttctgcga gatatcgttg aacgcgaaag
      541 aaccccagac caggcgcgcg gcaaaccacg cgaagtagcc cgacagcagc gtggcgatga
      601 ccaggcacag cacctccatc caccatcggg cgcgaccgtg cagacgttgg aggatcaggg
      661 tgacgcggat gtggtcgccc tgacgcaacg catagcccat cgcgagaaag gacccggccg
      721 ccaggaagta accggcatag gcgtccatcc cggccatgga gaaaccgagg tggcgaccgg
      781 cgatattgac gacctcgatc gccagcgtgg cgaagatgaa aaatgcgccc aggccgccca
      841 gcagcttgaa cagggggtcg aggaaggagc gcaccgtcgg tcgcgtggaa cggaagaaag
      901 ccgcttgggc ggccttcgcc ggtctatttg cggaactgcg tgatgatcgc ctggccgtcc
      961 ggtccggcct tggcgagcca ttcggtcagc agcgtttcgc cgagcttgtt cagttcggcc
     1021 ttgaacgccg cgttcggctc gtacacggtc atcttgttcg ccttcagcgt cgccagcgac
     1081 tcgccggtgt attcgcgcat gcgcttccag ccacgttcct cggcggccat ggcagcggcg
     1141 agaacggcgt cctgcgtcgc cttgtcgagc ttgtcgaatt ccttctggtt gacgatcacc
     1201 atgttcttcg gcagccacgc atcggcggtc gcgaagtgcg acagactctc ccacaccttc
     1261 gagtccacgc cggtagcgcc ggacgacatg aacgagtcga cgacaccagt ggcgagcgcc
     1321 tgcgacagct cggccgcctg gatcgtcagc ggctgcgcgc cgaccatggc ggcgatgcgc
     1381 gtcgtcgccg ggttgtaggc gcgccacttc agacccttca tgtcggccac cgagttcagc
     1441 tgcttcttgg tgtaaatgcc ctgcggcggc catgccacgg catagagcac cttgacgccc
     1501 tgggcggcca ggatcttctc cacgaccgga cgctgggcct gccacagctt ccacgaggcg
     1561 tcgaagccgg tagcaacaaa cggcacggca tccgcgccga acaccgggtt ctcattctcg
     1621 agcaagctca ttagcacttc gccgacctgc gcctggccgg tctgcaccgc gcgcttgatc
     1681 tcgggtgcct tgaagagcgc cccgccggcg tgcacggtga tgttcagctt gccgccagtc
     1741 gccttggcga cgtccgcggc gaactggttc aggttctgcg tgtgggggtt gccggcgggg
     1801 tagccagcgg gcaggtccca cttggtctgg gctgagaccg gcaaggccag taggaaggag
     1861 gctatggcgc tggcgccaag caacggcaga cgacgattca tgcagtcctc tcgtgcggga
     1921 tggttgcgga aagtgtggtt gcgaaaagta cgctaggtta gcgggagcga cgttcggcgg
     1981 ctaggatacg cgccaaccgg aggcagacgc gtggcccacg aacaatggga attctggatc
     2041 gatcggggcg gcaccttcac cgacatcgtc gcccgtcggc ctaacggctt gctggccacg
     2101 ttgaagctcc tgtcggaaaa cccggagcag taccgcgacg ccgcggtcgc cggcatccgc
     2161 cagctgctgt gcgtggccgc tgaggcgccg attccgttcg agcagatcgc cgcggtgaaa
     2221 atgggcacga cggtggcgac caacgcactg ctcgagcgca agggcgagcc tacggcgctg
     2281 gtcatctcgc gcggcttccg cgacgcgctg cgaatcgcct accagaaccg gccgcgcctt
     2341 ttcgaacggc acatcgtgtt gccggaactg ctctacgcgc gtgtgatcga ggccgacgag
     2401 cgcgtcgggg cccacggcga catcctgcag ccgctcgacg agacggcgtt ggccagcgaa
     2461 ctgcgggcgg cctacgcggc cggcctgcgc agctgcgcaa tcgtgctgat gcacggttac
     2521 cgctacaccg cgcacgagca ggccgcggcc cgcctcgcgc gcgaggccgg attcccgcag
     2581 gtgtcggtgt cgcacgaggt cagtccgctg atgaagctcg tcgcgcgcgg cgacacgacg
     2641 gtcgtcgatg cctacctctc gccgatcctg cgccgctacg tggagcaggt ggcgaccgat
     2701 ctgcccggcg cccggctgct cttcatgcag agcaacggcg gactgaccga cgcctggcgc
     2761 ttccagggca aggacgcaat cctgtcaggc ccggccggcg gcatcgtcgg catggcgcgg
     2821 gcgagccgcc acgccggctt cgatcgcgtg atcggtttcg acatgggcgg cacgtcgacc
     2881 gacgtgtcgc actacgccgg cgagtacgag cgcgagttcg agacgctcgt ggccggcgtg
     2941 cgcatgcgcg cgccgatgat gagcatccac accgtggcgg cgggcggtgg atcgatcctg
     3001 cacttcgacg gcgcgcgcct gcgggtcggc ccggattcgg ctggggccaa tcccggcccg
     3061 gcctgctacc ggcgcggtgg tccactggcg gtgacggatg ccaacgtcat gctcgggcgt
     3121 gtccagcccg gctactttcc gcccgtgttc ggtccgagcg gcctcgagcc gctcgatcgc
     3181 gacgtggtgg tccggcactt cagcgacctc gctcgggaga tcgagtcagc cactggccgg
     3241 acgcagacac cggagcaggt cgcaggcggt ttcgtcgaga ttgccgtcgg caacatggcc
     3301 gaggcgatca agcgcatctc ggtgcaacgc ggtcacgacg tcacgcgcta cacgctgacg
     3361 gtgttcggcg gcgcgggcgg tcagcacgcg tgcgccgtcg ccgatgctct ggcgatgacc
     3421 acggtctttg cgcatccgtt ggcgggcgtg ctgtcggctt acggcatggg cctcgccgac
     3481 cagacggcga tgcgtgagat gacgatcgag caaacgctgg tcgcggagac actgccgcac
     3541 ctggaggcaa gtctctcgac gctggcttcg caggccatgg cgtcgctgca atcccagggg
     3601 gttgcgcgcg agcggatcgg gctggtgcgg cgcgtgcacg tcaagtacga gggcacggac
     3661 accgctctgg cggtcgggta cggaacgata gacgcgatgc gcgcgcaatt cgatgcggcc
     3721 taccagcaac gcttttcctt cctgatgcct gaccgccgtc tgatcgccga aacggtctcg
     3781 gtcgaggcga ttggaggcgg cgatgcttct acctcgctgg ggtcggcaca gcggccggcg
     3841 cgtgcggggc cgctgaaggc ctcggccacc gtgcaggcgt tcttcgacaa cgcctggcat
     3901 gcaacaccgc tgtaccggcg cgacgatacg cagcccggcg atcatatcga cggcccggcg
     3961 atcgttgccg aagcgaacgc cacgaccgtc atcgaacccg gatggcaggc cgaggtgacg
     4021 ccgctcgacc atctcgtgct gcatcggatc gtgccgcgcg cagcacgccg cgcgatcggc
     4081 acgacggtcg atccggtgat gctcgagatc ttcaacaacc tgttcatgtc gatcgccgag
     4141 cagatggggc tgcggctgca gaacacggcc tactcggtca acatcaagga gcgactcgac
     4201 ttttcgtgtg cgctgttcga tgcgaccgga aacctgatcg ccaacgcacc gcacatgccg
     4261 gtgcacctgg gatcgatgag cgagtcgatc aagacggtga tgcgcgagaa cgccgggagc
     4321 atggttccag gcgacgcctt cgtgatcaac gacccgtacc acggggg
//
LOCUS       DATIRT010000241         1959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_724430, whole genome shotgun sequence.
ACCESSION   DATIRT010000241 DATIRT010000000
VERSION     DATIRT010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724430"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="VLN25_05600"
     CDS             complement(<1..1033)
                     /locus_tag="VLN25_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HSQ10072.1"
                     /translation="MPYAQFSSLLQRLRRPAPAPDRSVDVVDGSDGALAGDARFEPIA
                     LPGGHARFRSIALPLISVLVLVASWTVGVSYYFHQRSSTALFVAEQAAKAERAAAMVE
                     ASIAGDYRVVEQTARAMAARSDLITAMTRIDAEEAVRQWAQRSRRLGGDSAIEIYSSQ
                     GELVERIGDADGFRLAEPSDADEVRRALTGEDTLSVVERLQGLTLRAVVPVRSGSRIV
                     GAVGAERLIDAAYIGELATRLGLDVGLVARGRVLVGTAHADDRRWLEKIADQLANGAA
                     GSIALDGNLDVAVRPLSLSREPVAVAALMPNAQAYTALSGSLNAFVTVVLFTIFATIL
                     AGVYLTRYLI"
     gene            1285..>1959
                     /locus_tag="VLN25_05605"
     CDS             1285..>1959
                     /locus_tag="VLN25_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10073.1"
                     /translation="MLFTGATATTAPLAATSALMGPHIGIAAAWLLGAASVGALIALT
                     GVVLSQQLRARRELSAMRQTLAASATWSWRTDASGVVIEVERSHRTIDWFDCVALVGR
                     RPWEVRPGMTAPPALATAFAARAPYFDVLIEIESQDAGIRHIVTLSAAPMFSGTGKFV
                     GYAGAAHDLTPLLAGAGARPEVVSQLQSDLDERTRKLAGRSAELDHALRELDSFAYSV
                     SHDLRAP"
BASE COUNT          334 a          676 c          635 g          314 t
ORIGIN      
        1 gaatcaggta gcgcgtcaag tagacaccgg ccaggatggt ggcaaagatc gtgaacaaca
       61 cgactgtcac gaacgcattg agcgatccgc tcagggccgt atacgcctgc gcattcggca
      121 tcaacgcggc gactgcgacc ggctcacgcg aaagcgacag cggacggacg gccacgtcaa
      181 ggttgccgtc gagcgcgatc gacccggcgg cgccgttagc cagttggtcg gcgatcttct
      241 ccagccagcg gcgatcgtct gcgtgcgcag tgccaacgag tacccgtcca cgtgcgacaa
      301 gaccgacgtc cagcccgaga cgggtggcca gttcgccgat gtaagcggca tcgatcaggc
      361 gttcggctcc gacggctccg acgatgcggc tgcccgatcg caccgggacc acggctcgca
      421 gcgtcagacc ctgaagccgc tcgaccactg aaagcgtgtc ctcgccggtg agggctcggc
      481 gcacctcatc agcgtccgac ggctcggcaa ggcggaagcc atccgcgtcc ccgatccgtt
      541 cgaccagttc accctgagaa ctgtagatct cgatcgccga atcgccccca agtcgccgtg
      601 agcgctgcgc ccattgacgc acggcctctt cggcatcgat gcgcgtcatt gccgtgatca
      661 gatcggatcg tgccgccatg gcgcgcgccg tctgctcgac gacgcggtag tcacccgcga
      721 tggacgcttc gaccatggcc gcagcgcgct ccgccttcgc cgcctgttcc gccacgaaca
      781 gcgccgtgga gctgcgctgg tggaagtagt agctgacacc gacggtccag ctcgctacca
      841 ggaccagcac acttatcaac ggcaacgcga tcgaacggaa tcgggcatga ccaccgggca
      901 acgcaatggg ttcaaagcgg gcgtcgccgg ccagcgcgcc gtccgacccg tcgacgacat
      961 cgacgctgcg gtcaggcgcg ggcgctgggc gacgtagtcg ctgcaacaag gagctgaact
     1021 gcgcgtatgg catgggaagg cgcgatcgga cgcaccggtc ggatggtctc ggcaggctag
     1081 tctggaacgc tggggacaac ccctcgaaaa gccgtcccct ccgagcgcat ttcgggtgcc
     1141 ctatggacca cgctccgtac tcgcgccggc acccgcgttg ctgcacaatc gcactcggtg
     1201 gggaatgcac acattcacag gatctgctcg gacggacgcc cgctttcatg cgagcgggca
     1261 ctcgcacgcg ttcggagcgc tgtcgtgctc ttcaccggcg cgaccgctac gacagcgccg
     1321 ctcgcagcga cctcggccct gatgggaccg cacatcggaa tagcggccgc ctggttgctg
     1381 ggtgcggcct cggtgggtgc gctgattgcg ctgaccggcg tcgtgctgtc gcagcaattg
     1441 cgcgcgcgac gtgaactcag cgcgatgcgg cagaccctgg ccgcatcggc cacctggtca
     1501 tggcgcacgg acgccagtgg cgtcgtgatc gaggtcgaac gcagccatcg cacgatcgac
     1561 tggttcgact gcgtcgcatt ggtaggccgc cggccttggg aagtgcgtcc cgggatgact
     1621 gcgccgccgg cgctcgccac cgcctttgcc gcgcgcgccc cctacttcga tgtactcatc
     1681 gaaatcgagt cccaggacgc cggtatccgg cacatcgtga cgctgtccgc tgcgccgatg
     1741 ttctcaggca cgggcaagtt cgtggggtac gcgggggccg ctcatgacct cacaccgctg
     1801 ctggccggtg ccggggcacg ccccgaggtg gtctcccagt tgcaatccga tctggacgag
     1861 cgcactcgca agcttgccgg gcgctcggcc gaactcgacc atgcgctgcg cgaactcgac
     1921 agcttcgcgt actccgtctc gcatgacctg cgtgccccg
//
LOCUS       DATIRT010000242         4524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1510880, whole genome shotgun sequence.
ACCESSION   DATIRT010000242 DATIRT010000000
VERSION     DATIRT010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510880"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VLN25_05610"
     CDS             <1..524
                     /locus_tag="VLN25_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HSQ10074.1"
                     /translation="DEFRAVVGAVTPLAIKQRVEHDRPPHPPIPQKRIEDDQQVLIAS
                     VSDEFEIDTLLHTDAELSFRRPGIGPDVLRKLRRGEWVIQDHLDLHGARVDEARELLA
                     GFMREALRRGFRCVRVVNGKGLGSKDKMPVLKGKARVWLAQREEVIAFCQARPAEGGS
                     GALVVLLRPAQRP"
     gene            521..1162
                     /locus_tag="VLN25_05615"
     CDS             521..1162
                     /locus_tag="VLN25_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HSQ10075.1"
                     /translation="MIEFPTLLFVVEVVAVLACAFSGFAEAQRKDMDLVGVYVVAFIT
                     AFGGGTLRDVLLDRRPFFWVDHQQYAILILALTLIVTPLMRIAQRIMPNLAFVVADAI
                     GLGFFSITGVALALDSGMSPFVASVMGVVTGVFGGVLRDVVLNEVPMVLRDGRPYAIA
                     AFLGCWLYILMVNIGASSGPALWTAAGATTGFRLLAWRYNWSIKGANANGPRA"
     gene            complement(1170..1508)
                     /locus_tag="VLN25_05620"
     CDS             complement(1170..1508)
                     /locus_tag="VLN25_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HSQ10076.1"
                     /translation="MKQITAIIKPFKLDEVREALADVGVTGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKVEAVVPEALVERAIEAITKAARTGKIGDGKIFVRAVEQVIRI
                     RTGEEGEAAV"
     gene            complement(1542..3176)
                     /locus_tag="VLN25_05625"
     CDS             complement(1542..3176)
                     /locus_tag="VLN25_05625"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HSQ10077.1"
                     /translation="MNSNVKVAVAQINCSVGDFEGNSRKIVEAAARAVAGGADLLVTP
                     ELSLTGYPPEDLLLRRAFYAACDSALQKLAAQLLQFPELCVVVGHPLAREGQRFNAAS
                     VLQGGMVLGTYFKHDLPNYDVFDEQRYFTPDNRPFVFDVKGTAFGINICEDTWFHYAP
                     ECSLAAGAQVLLVLNASPFHLGKQAQRLQTMRDNVSHFGLAVVYANLVGGQDELVFDG
                     ASFVLAADGRLVASAPYFKESVLIIEFVDGVPKPGAIATEPSEEALAYDALVLGVRDY
                     VGKNGFPGALIGLSGGVDSALTLVVAVDALGADKVRAVMMPSQFTSPMSIEDAEQLAR
                     NLGVRHDVLPIKPAFDAFLATLSGEFRGLAWDTTEENLQARIRGTLLMALSNKYGAIV
                     LTTGNKSETAVGYSTLYGDLAGGFAVIKDILKTLVYQLCRHRNAGVEVIPERILTRAP
                     SAELKAGQTDQDSLPAYDILDDIITRYMQEGESLADIVGAGFDETVVRQVVRMIRTSE
                     YKRRQSPVGIRVTHRSFGRDWRYPITNRFREENASL"
     gene            3209..4345
                     /locus_tag="VLN25_05630"
     CDS             3209..4345
                     /locus_tag="VLN25_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSQ10078.1"
                     /translation="MRIVRDLSEVGADDWHALLRAASSKNPFVSFEFLHALHESGCAS
                     ARTGWEPHYLTLERGGRICAAVPLYRKHHSYGEYVFDWAWADAHQRHGLEYYPKWLSA
                     VPFTPVPGPRLIAIDAEARDQAARALVQLAQDSGLSSLHVLFPGEEEVSCLTNLGALL
                     RQTVQFHWFNRGYSTFDDYLGQLAQPKRKKVRAERRKVASEGVSVRRRVGRDITAADW
                     SLFASCYRSTYAAHHSTPYLNPRFFSRLATSMPDNLLLIIASRGGDDIATALALFDST
                     RLYGRYWGALATVPCLHFELSYYQLIEFAIERRLQVIEGGAQGEHKLARGFEPVKTWS
                     CHWLRHPDFAQAVERYLERESGGIEAYVDELNERSAFRTPAGPL"
     gene            4342..>4524
                     /locus_tag="VLN25_05635"
     CDS             4342..>4524
                     /locus_tag="VLN25_05635"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HSQ10079.1"
                     /translation="MTPLHLAAAVLMVTIWGFNFVVIRWGLNDIPPVLLTLLRFVLAA
                     FPAVLFVARPKAPWKLI"
BASE COUNT          769 a         1474 c         1437 g          844 t
ORIGIN      
        1 cggacgagtt tcgtgccgta gtcggcgccg tcacgccgct ggcgatcaaa cagcgcgtcg
       61 agcacgaccg gccgccgcat ccaccgattc cacaaaagcg tattgaagac gaccagcagg
      121 tcctgatcgc ttcggtgtcg gacgagttcg agatcgacac cctgctccat accgacgcgg
      181 agctgtcatt ccgacggccc gggatcggtc ccgatgtgct gcgcaaactc aggcgcggcg
      241 agtgggtgat ccaggaccat ctcgacctgc acggggcgcg cgtcgacgag gcacgcgaac
      301 tgctggcagg cttcatgcgc gaggcgctcc gacgcgggtt tcgctgtgtc cgcgtcgtca
      361 atggcaaggg ccttggatca aaggacaaaa tgccggtgct caagggcaag gcacgcgtct
      421 ggctcgcgca gcgtgaggag gtcatcgcct tctgccaggc gcgcccggcc gaaggcggca
      481 gcggcgcgct ggtggtgctg ctccgtcccg cacagcgacc gtgattgaat ttccgacgct
      541 gctgttcgtc gtcgaagtgg tcgcggtcct ggcctgcgcg ttttcagggt tcgccgaagc
      601 gcaacgcaag gacatggacc tggtcggcgt ctatgtcgtg gccttcatca cggcgttcgg
      661 cggtggcacg ctgcgcgacg tactgctcga tcgccggccc ttcttctggg tcgaccacca
      721 gcagtacgcg atcctgatcc ttgcgctgac gctgatcgtc acgccgctga tgagaatcgc
      781 acagcgcatc atgccgaacc tcgcgttcgt cgtcgctgat gccatcgggc tgggtttctt
      841 cagcatcacc ggcgttgcgc tcgcgctcga cagtggcatg tcgccgttcg tggcctcagt
      901 catgggcgtc gtgaccggag tcttcggcgg tgtcctgcgc gatgtcgtgc tgaacgaggt
      961 gccgatggtc ctgcgggatg gccggccgta cgcgatcgcg gcgttcctcg gctgctggct
     1021 ctacatcctg atggtgaaca ttggcgccag ttcgggcccg gccctgtgga ccgccgccgg
     1081 ggccacgacc ggtttccgcc tgctggcgtg gcgctacaac tggtcgatca agggcgcaaa
     1141 cgccaacggt ccccgggctt gacgcccgac tagacggccg cttcgccttc ctcgccggtg
     1201 cggatgcgta tgacctgctc gaccgcgcgg acgaagatct tgccgtcccc gatcttgccg
     1261 gtccgcgccg ccttcgtgat tgcctcgatc gcgcgctcga ccagggcctc gggcacgacc
     1321 gcctcgactt tcactttcgg caggaagtcg accacgtatt cggcgcctcg atacagctcc
     1381 gtatgaccct tctgtcgacc gaaacccttg acttccgtga cggtgaggcc ggtgacgccg
     1441 acatcggcga gcgcctcgcg cacttcgtcg agcttgaacg gcttgatgat cgcggtgatc
     1501 tgtttcatgg tcgcttccct ggcagctggt tcacgtcaat tctagaggct cgcgttttct
     1561 tcgcggaacc ggttggtgat cgggtagcgc cagtcgcggc caaaactgcg atgagtcacg
     1621 cgaatgccga ccggcgactg ccgccgcttg tactccgagg tccggatcat gcggacgacc
     1681 tgccggacga cggtctcgtc gaaaccagcg ccaacgatgt ccgcgagcga ctcgccttcc
     1741 tgcatgtagc gcgtgatgat gtcgtcgagg atgtcatacg caggcagcga atcctggtcg
     1801 gtctggcccg ccttcagctc ggcactcggc gcgcgcgtca ggatgcgctc gggaatgacc
     1861 tcgacgccgg cattgcgatg ccggcacagc tgatagacca gtgtcttgag gatgtccttg
     1921 atcacagcga aaccgccggc gaggtctccg tacagcgtcg aatagccgac cgcggtttcg
     1981 gacttgttgc cggtcgtgag gacgatcgca ccgtacttgt tggatagcgc catcagcagc
     2041 gtgccgcgaa tgcgcgcctg aagattttcc tcggtcgtgt cccacgccag accgcggaac
     2101 tcgcctgaca gcgtggccag gaacgcgtcg aacgcgggtt tgatcggcag cacgtcatgc
     2161 cgaacgccaa ggttgcgcgc cagctgctcg gcatcctcga tactcatcgg cgaagtgaac
     2221 tgcgacggca tcatcacggc ccgcactttg tcggccccca atgcatcgac ggcgacgacc
     2281 agggtcaagg ccgagtcgac accgcccgac aggccgatca gcgccccggg gaaaccgttc
     2341 ttgccgacgt agtcacgcac gccgagtacc agggcgtcgt aggcaagggc ctcctccgac
     2401 ggctccgtag cgattgcacc cggcttcggc acgccatcga cgaactcgat gatcagcacc
     2461 gactccttga agtacggtgc cgaggcgacc agacggccat cggccgccag cacgaaggaa
     2521 gcaccgtcga agacgagctc gtcctgtccg ccgacgagat tggcatacac caccgcgagg
     2581 ccgaagtgcg agacgttgtc ccgcatcgtc tgcagccgtt gggcctgctt gcccaggtgg
     2641 aacggcgacg cattcagcac cagcagtacc tgtgccccgg ccgccagcga acactccggc
     2701 gcgtagtgga accaagtgtc ctcgcagatg ttgatgccga acgccgtgcc tttgacgtcg
     2761 aatacgaacg gacggttgtc gggagtgaag tagcgctgct cgtcgaacac gtcgtagttg
     2821 ggcaggtcat gcttgaagta cgtgcccagg accatgcctc cctgcaacac cgaagccgcg
     2881 ttgaagcgct gcccttcgcg cgccaacgga tggccgacca ccacgcaaag ctccggaaac
     2941 tgcaggagtt gcgcagcgag cttctgcagc gcagagtcgc acgctgcgta aaatgctcga
     3001 cgcagcagca ggtcctcggg cggataaccg gtcagcgaaa gctcaggcgt gaccagaaga
     3061 tcggcaccgc ccgccacggc gcgggcagcg gcctcgacga tcttcctgct gttgccctcg
     3121 aagtcgccca ccgagcaatt gatctgtgct accgcgactt tgacgttcga gttcatgaat
     3181 gaatgcaaca ggttcggacc attattgcat gcgcatcgtg cgcgacctga gcgaggtcgg
     3241 ggccgatgac tggcacgccc tgctgcgcgc cgcgtcgtct aagaatccgt ttgtcagctt
     3301 cgagttcctg cacgccctgc acgagtccgg atgcgcctca gcacgcacgg gctgggagcc
     3361 gcactatctc acgctggaac gcggcgggcg gatatgcgcc gcggtgccgc tgtatcgcaa
     3421 gcaccactcg tatggcgaat atgtctttga ctgggcatgg gccgacgcac atcagcgaca
     3481 cggactcgag tactacccga agtggctctc ggccgtgccc ttcactccgg tgcccggacc
     3541 acgacttatc gcgatcgatg cggaagcgcg ggaccaggcc gcgcgggcgc tggttcagct
     3601 cgcgcaggat tcgggcctgt cgtcgctgca cgtgctgttc cctggcgaag aagaggtcag
     3661 ttgcctgacg aatctgggag cgctgctgcg tcagacggtc cagtttcact ggttcaatcg
     3721 cggctattcg accttcgatg actatcttgg ccagcttgcg cagcccaagc gcaagaaagt
     3781 ccgggccgaa cgccgcaagg tcgccagcga aggtgtcagc gtgcgccgcc gggtcggccg
     3841 cgacatcact gcggcggact ggtcgctttt cgcgagctgc tatcgcagca cttatgcggc
     3901 gcaccactcg acgccgtacc tgaacccgcg gttcttcagt cggctcgcga cctcgatgcc
     3961 ggacaacctg ctgctgatca tcgcttcacg cggcggcgac gacatcgcga ccgcactcgc
     4021 gctcttcgac tcgacccggc tctacggacg ctactggggc gctctggcaa cggtgccgtg
     4081 cctgcacttc gagctcagct actaccagct catcgagttc gcgatcgagc gtcggctgca
     4141 ggtcatcgaa gggggtgcgc agggagagca caaactggcg cgcggcttcg agccggtcaa
     4201 gacctggtcg tgtcactggc tccggcatcc ggacttcgca caggccgtcg agcgctatct
     4261 cgagcgcgaa agcggcggca tcgaagcgta cgtcgatgaa ctcaacgagc gttccgcttt
     4321 ccggacgccg gccggaccgc tatgacgccc ctgcaccttg ccgcggccgt gctaatggtc
     4381 acgatctggg gatttaactt cgtcgtaatc cgctggggtc tgaatgacat tccgcctgtc
     4441 ctgctgacat tgctgcgttt cgtgctcgcg gcgttcccgg cggtgttgtt cgtcgcgcga
     4501 ccgaaggcgc cgtggaagct gatc
//
LOCUS       DATIRT010000243         2482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_35463, whole genome shotgun sequence.
ACCESSION   DATIRT010000243 DATIRT010000000
VERSION     DATIRT010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35463"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VLN25_05640"
     CDS             <1..462
                     /locus_tag="VLN25_05640"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HSQ10080.1"
                     /translation="KHGELVAMLKSTLGMGHGDANTLVHTVRKSAGPAAVQGAPGGEV
                     LDGLYTGPKAALRLIHDKLMTALRKFGEFEEAPKKTYISYRRKKQFATIGPATNTRVE
                     VGLNMKGVKPTARLEELPAGQMCNYRVKLTDPAQVDAELVVWIRTAYDAAG"
     gene            complement(512..1024)
                     /locus_tag="VLN25_05645"
     CDS             complement(512..1024)
                     /locus_tag="VLN25_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10081.1"
                     /translation="MMVQNLQRVRYFFGKLLTVDDFQEEQSYFLNRLRRHNRFLHGWG
                     VVSGLGVSLYKANEVVVSAGLAIDCAGNEIALAEPVRIPLVAAKGTLHVAIRYQEQEV
                     GPVPAGNEVEYSRIREGSEVVILDVDPGTGHRGIGPGTPGCGLAHLLCLAKLTWHRSR
                     WQLRKVKNSA"
     gene            complement(1177..1644)
                     /locus_tag="VLN25_05650"
     CDS             complement(1177..1644)
                     /locus_tag="VLN25_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3)-I family aminoglycoside
                     N-acetyltransferase"
                     /protein_id="HSQ10082.1"
                     /translation="MPRSTSFSVRALTAADVDLMRALLTIFAEAFDEVATYSEAPPDA
                     GYLERLLGSENIMVLAAMKDGQVVGGLVAYELQKFEQMRSEIYLYDLAVAAPHRREGI
                     ATALILHLRKLAALRGAEVIFVQADLEDEPAIALYTKLGIREDVLHFDIPAAR"
     gene            complement(1787..>2482)
                     /locus_tag="VLN25_05655"
     CDS             complement(1787..>2482)
                     /locus_tag="VLN25_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10083.1"
                     /translation="LRSEEKSNGFVEKSFFEYHLYTLGRTTTLPDNSTKQIELFPAAR
                     RVPCEKLLVYYGAPSGHRGFLSSPATDRNYGVQSNRKVDVYLQFTNAEASGMGMPLPA
                     GRVRVSKLDPADQTLEFIGEDAIDHTPRNEKVLLKLGSAFDVVGERRQLDFRVDTSRR
                     TMTEEIEVRLRNQKKERVEVMVKENLYRWVNWSITSKSHDYRKEDARTIVFPVTIAPE
                     SEVVVRYTANYSW"
BASE COUNT          459 a          755 c          810 g          458 t
ORIGIN      
        1 aagcacggcg aactcgtcgc catgctcaag tcgacgctcg gcatgggcca cggcgatgcc
       61 aacacgctgg tgcacacggt acgcaaatcc gccgggccgg ccgcggtgca gggagcgccg
      121 ggcggcgagg tgctcgatgg cctgtacacc ggaccgaagg cggcgctgcg gctgattcac
      181 gacaagctga tgacggcgct gcgcaaattc ggagagttcg aagaggcacc gaagaagacc
      241 tacatcagtt accggcggaa gaaacagttc gcaacaatcg gaccggccac caatacgcgg
      301 gtcgaggtcg gtctcaacat gaagggcgtg aagccaaccg cgcggctgga ggaactgccg
      361 gccgggcaga tgtgcaacta ccgtgtcaag ctgaccgacc cggcgcaggt cgacgcggaa
      421 ctcgtcgttt ggatcagaac tgcgtacgac gcggcaggtt gaacgttcag acgtgcaatg
      481 acggcgcgga gggccgtcgg cgtctagccg gtcaggccga atttttgacc ttgcggagct
      541 gccacctgga ccgatgccac gttagttttg caagacacag caggtgagct agaccgcagc
      601 cgggtgttcc tgggccgatc ccgcggtgcc cagtgccagg atcgacatcg aggatgacga
      661 cctccgaccc ttcgcggatg cgtgagtact cgacttcatt tcctgccgga accggaccga
      721 cttcctgttc ctgataccga atggccacgt gcagcgttcc tttggcagcg accagtggaa
      781 tgcgcacagg ctccgcgagg gcgatttcgt tgcctgcaca gtcgatggca aggcctgccg
      841 agaccacgac ttcgttggcc ttgtagaggc tcaccccaag gccactgaca acaccccacc
      901 catgcaggaa ccggttgtgg cggcgcagac ggttcaggaa gtaagactgc tcctcctgaa
      961 agtcatccac cgtcagcaac ttgccaaaga agtagcggac ccgctgcagg ttttgaacca
     1021 tcatcgacgt gacctccgac ggggaatggg gccgagctta cttgggtcag tgatcgagct
     1081 atgcgagaga tgagggccgt ggtgaggaag ctttccagcc cagcggcact cggttggctg
     1141 gtcccgtgcc tttgccgctc ccgccgggtg gagcgcctaa cgggcagcgg gtatgtcgaa
     1201 gtgcaggacg tcctcgcgaa ttcccagctt ggtgtagagc gctatggccg gttcgtcctc
     1261 gagatcggcc tgcacgaaaa tcacctcggc gccgcgcagt gcggcaagct tcctgaggtg
     1321 caggatcagt gccgtggcga tgccttcgcg ccgatggggt gcagcaaccg caagatcgta
     1381 gaggtagatc tcgctgcgca tctgctcgaa cttctgcaac tcataggcca ccaacccacc
     1441 cacaacctgg ccgtctttca tcgcagcgag gaccatgatg ttctcgctgc cgagcagacg
     1501 ctccaggtag ccagcatcgg gcggggcctc gctgtaggtc gcgacctcgt caaaggcctc
     1561 ggcaaatatc gtcagcagtg cgcgcatcag gtcgacgtcg gcggctgtga gggcgcggac
     1621 ggaaaaggac gtcgatcggg gcatgctcgc gttcgatgaa tctgagtgga gttggctggg
     1681 ctcgctgccg cactgtaccg gcgagtgggg cggcatgcgt ccgcactcgg ccggcagtcg
     1741 gtgggtggct ggtcagcgaa tggccgcccg gagccgttcg tcccagctac cagctgtagt
     1801 tcgcggtgta gcgaaccacg acctcgctct ccggcgcgat ggtgactgga aagacgatcg
     1861 tccgcgcgtc ctccttgcgg tagtcgtgtg actttgacgt gatgctccag ttgacccagc
     1921 ggtagaggtt ctccttcacc atcacctcga cgcgctcctt cttctggttg cgtagccgaa
     1981 cttcgatttc ctccgtcatc gtcctgcgcg acgtgtccac acgaaagtcg agctgccgac
     2041 gctcgccgac gacgtcgaag gccgaaccga gcttgagcag cactttttcg ttgcgcggcg
     2101 tgtggtcgat cgcgtcttcg ccgatgaact cgagtgtctg gtcggcggga tcgagcttgg
     2161 acacgcgcac gcgccccgcg ggcagcggca tgcccatgcc gctcgcctcg gcgttggtga
     2221 actgcaggta gacatcgacc ttgcgattgc tctgcacgcc gtagttgcga tcggtggccg
     2281 gcgacgagag gaagccgcga tgcccgctcg gcgcgccgta gtagaccagc agcttctcgc
     2341 acggcacgcg acgcgccgcc ggaaacagtt cgatctgctt ggtcgagttg tcgggcaggg
     2401 tggtggtgcg gcccagcgtg taaaggtggt actcgaagaa cgacttctcg acgaagccgt
     2461 tggacttttc ctcgcttctc ag
//
LOCUS       DATIRT010000244         3459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_133856, whole genome shotgun sequence.
ACCESSION   DATIRT010000244 DATIRT010000000
VERSION     DATIRT010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133856"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..1303)
                     /gene="amrS"
                     /locus_tag="VLN25_05660"
     CDS             complement(188..1303)
                     /gene="amrS"
                     /locus_tag="VLN25_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HSQ10084.1"
                     /translation="MTHTITRLPPSSLPDEHYPARWWHRLDDGRIQCDLCPRDCRLHD
                     GQRGLCFVRKREGEAMVLTTYGRSSGFCVDPIEKKPLNHFHPGSAVFSFGTAGCNLAC
                     KFCQNWDISKSTEMDRLMDQASPDAIAQAALASGCGSVAFTYNDPVIFAEYALDTADA
                     CHANGLKTVAVTAGYIHAEPRREFYSKMDAANVDLKAFTEEFYFKLTGAHLAPVLDTL
                     KYLVHETECWTEITTLLIPTKNDSDAELRAMCQWIARELRRDVPLHFTAFHPDYKLND
                     LPATPMSTLARARRIAHEEGLQYVYTGNVHDREGGTTFCPACHAVLIERDWHRIVRHE
                     LTDDGRCPHCATAVAGRFGGFEGGFGNRRIPVRIALR"
     gene            complement(1300..2796)
                     /gene="amrB"
                     /locus_tag="VLN25_05665"
     CDS             complement(1300..2796)
                     /gene="amrB"
                     /locus_tag="VLN25_05665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HSQ10085.1"
                     /translation="MTSVIKPAAPQEAMPSRPPAVAGLFYPDDPIALRRDVQQFVAAA
                     GALAPSAAAPKALIAPHAGYVYSGAIAGQAYARLLPWAGRIRRVVLLGPVHRVSVRGL
                     ALPSDRRFATPLGSVEVDREAVAGLRDLPQITVNDRAHAAEHSLEVHLPFLQQVLGHF
                     TLVPLAVGDATPDEVAEVLALLWGGEETLIVVSSDLSHYLPYATARAADRATVDTMLA
                     LDPTLDHAQACGATPVNGLLKLARRLGLRAELLDLRNSGDTGGDRGRVVGYAAIALVE
                     PTAARPSKGDEVAVTADASGTADPADTRSSTLLAHARSAIAHRLGVPSVPTPQASFLA
                     DPGATFVTLKVNGELRGCVGSLQPRRPLGEDVRANAQAAAFDDPRFPPLDCAEYDALE
                     VEVSVLSECAPIVVASERELHAQLRPGIDGVTLQWGSRRATFLPQVWESLPDPREFLG
                     QLKRKAGLPLDFWSDELSISRYTVEKFTEEKVTEDKFVEDKFVEGQAA"
     gene            complement(2923..3306)
                     /locus_tag="VLN25_05670"
     CDS             complement(2923..3306)
                     /locus_tag="VLN25_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSQ10086.1"
                     /translation="MLTHSPATTILPVRDLARAREFYQQQLGLTPKGLQQDGKFLFAL
                     GDGGTLALFPKPDGTKAEHTAVSFKVDDVVGEIRALEAKGVVFEDYDFPGFRTEGHIC
                     VLGSEKAAWFKDPEGNILCLHEDVP"
BASE COUNT          594 a         1151 c         1157 g          557 t
ORIGIN      
        1 gtttctcgcg ggccagcacg agatcgagcg ggcgcgccgg gtgctgctgc agatgaggga
       61 gctgcccaaa tccgagcgtg tgcgctgggc cggcaagccg ctgctgcttg cggcgccggc
      121 gaagacgcca ggagcgaccg acagtcccac cgcggcagcg cgttcgccgg cggctccgac
      181 gaacccgtca gcgcagcgcg atgcggaccg ggatgcgccg attgccgaac ccgccctcga
      241 atccgccaaa gcggccggcc accgccgtcg cgcagtgcgg gcagcggccg tcgtcggtga
      301 gctcatggcg cacgatgcga tgccagtcgc gctcgatgag caccgcatga cacgccggac
      361 agaacgtggt tccgccttcg cggtcgtgca cgttaccggt gtacacgtac tgcagccctt
      421 cctcgtgggc gatgcggcgc gcacgggcga gggttgacat cggcgtggcc ggcaggtcat
      481 tgagcttgta gtccgggtgg aaggcagtga agtgcagcgg cacgtcgcgc cgcagttcgc
      541 gcgcgatcca ctggcacatc gcgcgcagct cggcatcgga gtcatttttc gtcgggatca
      601 gcagcgtggt gatctcggtc cagcactcgg tttcgtgcac caggtacttc agcgtgtcga
      661 gcacgggagc gaggtgcgca ccggtgagtt tgaagtagaa ctcctcggtg aacgccttca
      721 ggtcgacgtt agccgcgtcc attttcgagt agaactcgcg ccgcggttcg gcatggatgt
      781 aacccgccgt cacggccaca gtcttgaggc cgttcgcgtg gcaggcgtcc gccgtgtcga
      841 gcgcgtactc ggcaaagatc accggatcgt tgtaagtgaa ggccacgctc ccgcagccgg
      901 acgcaagcgc ggcctgcgcg atcgcgtcgg gactcgcctg gtccatcagc cggtccattt
      961 cggtggactt ggagatgtcc cagttctggc agaacttgca ggcaaggttg cagccggcgg
     1021 tgccaaagga aaacacggcc gagcccggat ggaagtggtt aagtggcttc ttctcgatcg
     1081 gatcgacaca gaatcccgag gagcgcccgt aggtggtgag aaccatcgcc tcgccttcgc
     1141 gcttgcgcac aaaacacagc ccgcgctggc cgtcgtgcag cctgcagtcg cgcgggcaca
     1201 ggtcgcactg gatgcggccg tcgtcgagcc gatgccacca gcgcgccggg taatgttcat
     1261 ccggcagcga tgacggcggg aggcgcgtga tcgtgtgcgt catgccgcct gtccttcgac
     1321 gaacttatct tcgacgaact tatcttcggt gaccttctct tcggtgaact tttcgacggt
     1381 gtaccgcgag atcgagagtt cgtctgacca gaagtcgagc ggcagtcccg ccttgcgctt
     1441 cagctggcca aggaactcgc gtgggtccgg caggctctcc cacacctgcg gaaggaaggt
     1501 ggcgcggcgg ctgccccatt gcaacgtcac gccatcgatg cccggccgca actgcgcgtg
     1561 cagttcgcgc tcgctggcca cgacgatggg cgcgcactcg gacagcaccg agacctccac
     1621 ctcgagtgca tcgtattcgg cgcagtccag tggcggaaaa cgcggatcgt cgaacgcggc
     1681 cgcctgtgcg ttggcgcgca catcctcgcc caacgggcgg cgaggctgca gtgatccgac
     1741 acagccgcgc aactcaccat tgaccttcag cgtgacgaag gtcgctcccg ggtcggcgag
     1801 aaaagacgcc tgcggcgtcg gcaccgacgg cacgccgagc cgatgtgcga tcgccgagcg
     1861 tgcatgcgcg agcagcgtcg agctgcgggt atcggccggg tcagccgtac cggacgcatc
     1921 ggccgttacc gccacttcat cgcccttcga cgggcgagcc gcagtcggtt cgaccagtgc
     1981 gatcgcggca tagccgacca cgcggccgcg gtcgcccccg gtgtcacccg agttgcgcag
     2041 atcgagcagc tcggcgcgca agcctagccg ccgcgccagc ttgagcaggc cgttgaccgg
     2101 cgtggcgccg cacgcctgcg catgatccag cgtcgggtcg agcgccagca tcgtatcgac
     2161 ggtggcgcgg tcggccgcac gcgcggtggc gtacggcaga tagtgagaca ggtcggaact
     2221 cacgacgatc agcgtttctt caccgcccca tagcaaggcc agcacctcgg cgacctcgtc
     2281 aggcgtcgcg tcgccgacag cgagtggcac cagcgtgaag tgaccgagca cctgctgcag
     2341 gaacggtaga tgaacctcga gcgagtgttc cgccgcgtgc gcgcggtcat tgacggtgat
     2401 ctgggggagg tcgcgcagcc cggccaccgc ctcacggtcg acttcgaccg agccgagcgg
     2461 agtggcgaaa cgtctatccg acggcagcgc gagcccgcga accgatacgc gatgcaccgg
     2521 gccgagcagg acaacgcggc gtattcgccc ggcccagggc agcagccgcg catacgcctg
     2581 gccggcaatc gcaccggagt agacgtagcc cgcgtgcggc gcgatcaggg ccttgggggc
     2641 cgccgcggag ggagccagcg ctccagctgc agccacaaac tgctgcacat cacggcgcaa
     2701 tgcgatcggg tcgtccgggt agaacaggcc ggccaccgcc ggcggccggc tcggcatcgc
     2761 ctcctgcggc gccgccggtt tgatcacgga tgtcatgaac tggcggcctc tctgcggccc
     2821 acactattac agcctatgcc gttggtgtgg ggccgatcac gcttcgatca agaggcggcg
     2881 cgagacggtc cgcgcatgct tggccagtcg acaacagtcg gcctacggca catcctcatg
     2941 caggcaaagg atgttgccct cggggtcctt gaaccaggcg gctttttccg aacccagcac
     3001 acagatatgc ccttcggtcc ggaaacccgg aaagtcgtag tcctcgaata cgacaccctt
     3061 tgcctccagc gcgcggatct cgcccaccac gtcgtcgact ttgaagctca cggccgtgtg
     3121 ctcggccttg gtgccgtcgg gcttaggaaa gagcgcgagc gtgcccccat cgccgagcgc
     3181 aaacaggaac ttgccgtcct gctgcaggcc cttgggggtc agcccgagct gctgctgata
     3241 gaactcgcgt gcgcgcgcca gatcacgcac cggaaggatc gtcgtggcgg gagagtgagt
     3301 cagcatcgga acttcctcga ggcgtgggac ccgcacgatg gcactgcgcg cactctcacg
     3361 caatctgcag tgcacgaccc gtggccgcga cgatcgctac agtccttcgc gccactcggc
     3421 attgaagacc ttccagcgtc cctgctcgcg catccacac
//
LOCUS       DATIRT010000245         4640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1314704, whole genome shotgun sequence.
ACCESSION   DATIRT010000245 DATIRT010000000
VERSION     DATIRT010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1314704"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..512
                     /locus_tag="VLN25_05675"
     CDS             21..512
                     /locus_tag="VLN25_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HSQ10087.1"
                     /translation="MKQLLIVFHSLTGAAEQLAVAAAAGAREEAEVETRLLTAASAGV
                     DDMLHADGYLFATPENLATMAGLMKDFFDRVYYPVLDRINGRPYAAIIAAGTDGTGAA
                     RQVQRIATGLRLREIAPPLVVRNGAQTPEEILALKIVPAIDLAHARELGSGLAAGLAS
                     GIF"
     gene            complement(531..1400)
                     /locus_tag="VLN25_05680"
     CDS             complement(531..1400)
                     /locus_tag="VLN25_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010092563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSQ10088.1"
                     /translation="MEPRSHHVNCLSPAGFHRMAYVEWGDADNPKVLLCVHGLTRMGR
                     DFDRLARAMADRYRIVCPDVVGRGHSEWLRESAFYAIAQYASDMVALIARLDVEQVDW
                     VGTSMGGLIGIALAGQVASPIRQLVLNDVGPRLGPLAIARIGSYVGQPVSFASMEEAI
                     DYQSVTAAPFGLRTREDWRELVTPSMRQVDGRYVFRYDPAIAVPFKSATPQVAAAGEA
                     ATWALYDRITARTLLVRGAQSDLLTPETAAEMSARGPRPRVAEIPDVGHAPTFMHESE
                     IQLVREFLLDAAA"
     gene            1488..2267
                     /locus_tag="VLN25_05685"
     CDS             1488..2267
                     /locus_tag="VLN25_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HSQ10089.1"
                     /translation="MLKNRTALVTGSTSGIGLAIADALATQGANIVVNGFGEPEAIRA
                     AETQLQKHGVTTMYHGADMSKPAEIEQMIRAAEGTFGRIDILVNNAGIQHVSPVESFP
                     VERWDAIIAINLTSAFHTIRLALPKMKQANWGRIINIASLHGLVASVDKSAYVAAKHG
                     IVGLTKVVALETATTGVTCNAICPGWVLTPLVQKQVDARAARDNLGQEEAKKALLREK
                     QPSMQFVTTEQLAGLAVFMCSPAADQVRGVAWNVDGGWFAQ"
     gene            complement(2281..3183)
                     /locus_tag="VLN25_05690"
     CDS             complement(2281..3183)
                     /locus_tag="VLN25_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003277048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HSQ10090.1"
                     /translation="MSAAVELHVSHRTVTRYSSRVEVAYHCAFLHPRDGGRQKVRDFA
                     MSIDPQPSHRATAPDSFGNLRTEFALYVPHDALIVHTESRVWLEPRPAPPDAAASTAW
                     DTVAAGLRYVLGGSFQAAGEFVFASPYVPLDRSLRDYAATDFRAGRPYLQAAIALMER
                     VHADFAYEPGVTDIGTPLIDVFRQRRGVCQDFAHLMLGMLRSLGLPARYVSGYLLTQP
                     LPGKARLTGADASHAWVSIWCPALGWVDLDPTNAVLPDASHVTVAVGRDYGDVVPLRG
                     VIRGGADHVLDVAVDVVPVVERAA"
     gene            complement(3180..>4640)
                     /locus_tag="VLN25_05695"
     CDS             complement(3180..>4640)
                     /locus_tag="VLN25_05695"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HSQ10091.1"
                     /translation="LASWWCGERAAFEQVAGELSDKVIKATFPSSQPRPAFEPMIGAA
                     MGASQLRDVRRLIEADPDAYTVQAYLPLPQAPTWHDGSLVPRAAMVRVFVINDGSGGW
                     HVMPGGLTRIAQREQRVVSMHRGGASQDTWVQTAGPVDTFSMLPTPLRPEELVGRRRI
                     VSSRAAENLFWMGRYAERADTSVRLARSALTLLGDDARAPSPVLGALATMCEEQGLVP
                     HDVPSPSQSVTVFERTLIAGLADADERPSVAFTLEALVRSAGHIRDRLSADHWRLIAA
                     TSQRFRADCAAASADQVFSPDEAVAALGHLAVQLSAITGAQVDHMTRDDGWRLLTIGR
                     QLERLGTLARALQVLFSTGAAARDDGFDLILQLFDSTITYRALYQRRLEAAPLIDLLV
                     QEQANPRSLRGVARRLGEQIERLGAPGAHELLLHLPAQLDWPNLESLCRPDSSGQNAA
                     LLNFCERLFDGSRALSEAIGARYFSHASAEFRTVHA"
BASE COUNT          850 a         1566 c         1530 g          694 t
ORIGIN      
        1 caggatccgc tggcggcgtc gtgaagcagc tcctgatcgt attccactcg ttgaccggag
       61 cggccgagca actcgcggtc gccgcggccg ccggagcgcg tgaagaagcg gaggttgaaa
      121 cgcgactcct caccgcagcc agcgccggcg tcgacgacat gctgcacgca gacggctacc
      181 tgtttgccac gcccgagaac ctggcgacga tggcagggct gatgaaggac ttcttcgatc
      241 gcgtgtacta cccggtcctc gaccgaatca atggccggcc ttatgcagcg atcatcgcgg
      301 cgggaaccga cggaacgggg gccgctcgcc aggtacagcg catcgccaca gggttgcgac
      361 tgcgcgagat cgcgcccccg ctcgtggtcc gcaacggggc tcagacgccc gaggaaatcc
      421 tcgcactgaa aatcgtcccc gcgatcgacc tcgcacacgc acgcgaactg ggctccggcc
      481 tcgctgccgg cctggccagc ggtattttct aggcggcttt caggcgatct ttaagcggct
      541 gcgtcgagca ggaattcgcg gaccagctgg atctcgctct cgtgcatgaa tgtcggagcg
      601 tgacccacgt ccggaatctc ggccacacgg ggacgcggcc cgcgggccga catctcggca
      661 gcagtttcgg gcgtcagcaa gtccgactgt gccccgcgga ccagcaacgt gcgcgcggtg
      721 atgcggtcat agagcgccca cgtcgccgcc tcaccggccg cagcgacctg cggcgtcgcg
      781 ctcttgaacg gcaccgcgat cgccgggtcg tagcggaaca catagcgccc gtcgacctgg
      841 cgcatcgacg gcgtcacaag ttcgcgccag tcctcccggg tcctgagccc gaacggcgca
      901 gctgtcaccg actggtagtc gatagcctcc tccatgctcg cgaaggacac cggttgcccc
      961 acataactgc cgatgcgcgc aatcgccagc ggaccgagtc ggggcccgac gtcgttgagc
     1021 acgagttgtc ggatcgggga agccacctgg ccggcgagcg cgatgccgat cagcccgccc
     1081 atggaagtgc cgacccagtc gacctgctcg acgtcgagcc gtgcgatcag cgccaccatg
     1141 tccgaggcat attgcgcaat cgcgtagaac gcggattcgc gcagccattc gctgtgaccc
     1201 cggccgacca cgtccggaca gacgatccgg taacgatctg ccattgcacg cgccagccgg
     1261 tcgaagtcgc ggcccatccg cgtcagcccg tggacacaga gcagcacctt gggattgtcg
     1321 gcgtcgcccc actcgacgta ggccatgcgg tgaaagccgg ccgggctgag gcaattgacg
     1381 tggtgcgatc ggggttccat gggacgcgcg acttggtctg ctgaggaagc tgcccattgt
     1441 agtcagcggc agatggcata ctttccagga ccacaacgga gatgcgcatg ctgaagaaca
     1501 ggacggcgct ggtgaccgga tccaccagcg gtatcgggct ggcgatcgca gacgctttgg
     1561 cgacccaggg tgcaaacatc gtcgtcaacg gcttcggcga gcccgaagca atccgcgcgg
     1621 cagaaacaca gctgcagaag cacggcgtga caacgatgta ccacggtgcc gacatgagca
     1681 agccggccga aatcgagcag atgattcgcg cggccgaggg caccttcggc cgcatcgaca
     1741 tcctggtgaa caacgccggc atccagcacg tttcgccggt ggaaagcttt ccggtcgaac
     1801 gctgggacgc aattatcgcc atcaacctga cgtcggcgtt ccacacgatc cggctggcat
     1861 tgccaaagat gaagcaggcc aactggggcc gcatcatcaa catcgcgtcg ctgcacggac
     1921 tggtcgcgtc ggtcgataag tcggcttatg tcgccgccaa gcacggcatc gtcggcctga
     1981 ccaaggtggt tgcgctggaa acggcgacga ccggagtcac ctgcaatgcg atctgtccgg
     2041 gttgggtttt gacgccgctg gtgcagaaac aggtcgatgc acgcgcggcg cgcgacaatc
     2101 tgggtcagga ggaggccaag aaggcactgc ttcgggaaaa gcagccgtcg atgcagttcg
     2161 tgacgaccga acagcttgcc ggcctcgcgg tcttcatgtg ctcgcccgcg gcggatcagg
     2221 tgcgcggagt ggcctggaat gtcgacggcg gctggttcgc gcagtaacgg gcgacttgcc
     2281 tcacgcggcg cgctcgacca ccggtacgac atcgaccgcg acgtcgagca cgtggtccgc
     2341 gccgccccga atgacgccgc gcagcggaac gacatcgccg tagtcacggc cgacagccac
     2401 ggtcacgtgg ctcgcatcgg gtagcaccgc gttggtcgga tcgaggtcga cccagccgag
     2461 cgccggacac cagatcgaca cccaggcgtg cgaggcatct gcgcctgtca gacgcgcctt
     2521 gcccggcagc ggctgcgtca gcagatagcc gctgacatag cgagcaggca gtccgagcga
     2581 gcgcaacatc cccagcatca ggtgcgcgaa gtcctggcac acgccacggc gctgccggaa
     2641 cacgtcgatc agcggcgtgc cgatgtccgt cactcccggt tcataagcaa agtcggcgtg
     2701 cacccgttcc atcagcgcga ttgccgcctg caaatagggg cggcccgccc ggaagtccgt
     2761 tgccgcatag tcgcgcagcg aacggtcgag tggaacgtaa ggtgatgcaa acacgaactc
     2821 gcccgccgct tgaaacgagc cgccgagcac gtagcgcagg cccgccgcga ccgtgtccca
     2881 ggcagtactc gctgcagcat ctggcggcgc ggggcgcggc tcgagccaca cccgactttc
     2941 cgtatgcacg atcagcgcgt catgcggcac gtacagcgcg aactcggtcc gcagattgcc
     3001 aaaggagtcc ggcgcagtcg cgcggtgcga cggctgtgga tcgatcgaca tcgcaaagtc
     3061 cctgaccttc tgccgcccgc catcgcgcgg gtgcaagaag gcgcagtgat aggccacctc
     3121 gacgcgcgag gaatagcgag tgacggtccg gtgcgagaca tgaagttcga cggccgcgct
     3181 catgcgtgca ccgtccggaa ctctgccgat gcatggctga agtagcgcgc accgatggcc
     3241 tccgacaggg cccgactgcc gtcgaacagc cgctcacaga aattcaacaa cgctgcgttc
     3301 tgccccgaac tgtccggccg gcacagcgat tcgaggttcg gccagtcaag ctgcgcgggc
     3361 aggtgcagca gcagttcgtg cgcacccggg gccccgagtc gttcgatctg ctcgcccagg
     3421 cggcgcgcca cgccgcgcaa cgaccgtggg ttcgcctgct cctgaaccag aaggtcgatc
     3481 aacggcgccg cttccagtcg gcgctggtag agcgcgcggt aggtgatcgt gctgtcgaac
     3541 agctgcagga tcaggtcgaa tccgtcgtcg cgcgccgcgg cgccggtcga aaaaagaacc
     3601 tgcagcgcac gtgcaagcgt gcccaggcgc tcgagctgcc gcccgatcgt cagcaagcgc
     3661 cagccgtcgt cgcgggtcat gtgatcgacc tgcgcgccgg tgatggccga cagttgcacg
     3721 gccagatgtc ccagggccgc cactgcctcg tcgggcgaaa acacctggtc ggcggaggcg
     3781 gcagcgcagt ccgcacggaa tcgttgtgac gtggcggcga tcaggcgcca gtgatccgcc
     3841 gacagccgat cgcggatgtg accggcggat cgaaccagcg cttcgagcgt gaacgccaca
     3901 ctcggcctct cgtccgcatc ggccaggccc gcgatcagcg tgcgctcgaa cacggtcacc
     3961 gactgtgagg gcgacggcac gtcgtgcggc accaggccct gctcttcgca catcgtcgcc
     4021 agcgcgccga gcaccggcga tggagcgcgc gcgtcgtcac ccagcaaggt caacgcggag
     4081 cgggccagcc gcacggacgt atcggcgcgc tcggcatagc ggcccatcca gaagagattc
     4141 tcggccgcgc ggctcgagac gatgcgccgc cgcccgacca gttcctcggg gcgcagtggc
     4201 gtcggcagca tcgagaacgt gtcgaccgga ccggccgtct gcacccaggt gtcctggctc
     4261 gcgccgccgc gatgcatcga caccacccgc tgttcgcgct gcgcgatgcg ggtgagtcct
     4321 cccggcatga catgccaccc tccactgccg tcgttgatca caaacaccct caccatggcc
     4381 gcgcgcggca ctagtgaacc gtcgtgccac gtgggggcct gcggcagcgg caggtaggcc
     4441 tgcaccgtat aggcatccgg gtcggcttcg atgagccggc gcacgtcgcg caattgcgag
     4501 gcgcccatcg cggcgccgat cattggttca aatgccgggc gtggctgcga cgacgggaac
     4561 gtcgccttga tgaccttgtc ggatagctcg ccggcgacct gctcgaaggc ggcacgctca
     4621 ccacaccacc atgacgcgag
//
LOCUS       DATIRT010000246         2445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1413487, whole genome shotgun sequence.
ACCESSION   DATIRT010000246 DATIRT010000000
VERSION     DATIRT010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413487"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1450
                     /locus_tag="VLN25_05700"
     CDS             <1..1450
                     /locus_tag="VLN25_05700"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HSQ10092.1"
                     /translation="PEEAIDFTYQVDLTRCKAHRACVNACGAIGAIDFSRPASNRTEQ
                     FDLVFDLSAQPLLNMPEKPFGYQAPGADPLEQAQAATRLVQMVGEFEKPRYVQYDEKL
                     CAHSRNTKTGCTRCLDVCATDAVSSLGDKIWAHPFLCMGCGDCATTCPTGALHFAYPA
                     VADIGTRLRKLLAVYRGAGGKDAALLFHNGTSGREQIARLARVGKGLPARVLPIEVHS
                     VATIGIDVVLGAIALGASQVVSLLDDTGADEHGALLDQQARIAGAILNGLGYSGTHWQ
                     TLRAEDAAALQAALWSSQPAATVAEPATFNLSNRKRETLDFAIHHLVQQASVPPDEIA
                     LPAGSPFGTLVVDASRCTLCKACIGACPSSALQDAEDAPKLRFIERNCVQCGLCETTC
                     PEQAISLVPRLLVTPAARQPVTLNEAEPFHCVRCGTPFGTRQMIDVMTGRLGAHSMFA
                     GAALRRIQMCADCRVIDMMEAKNEVSIFDVKR"
     gene            1510..2061
                     /locus_tag="VLN25_05705"
     CDS             1510..2061
                     /locus_tag="VLN25_05705"
                     /inference="COORDINATES: protein motif:HMM:NF014651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HSQ10093.1"
                     /translation="MYALLARVFAAGPDEGLLRALAQSAGSVEGDGELAQAWNDLAAA
                     AAHTSARDATLEFDETFIGVGKAPVSTYLSHYHTAAHKEAMLVELRDTLVQLGLARTV
                     ASAEPEDNLTAVLEVMRHLVAAGSDEKSLQRQREFFVRYLQPVYREFCDAAHKADLSA
                     FYKAAVRILEVFMVSDLAQFEMG"
     gene            2147..>2445
                     /locus_tag="VLN25_05710"
     CDS             2147..>2445
                     /locus_tag="VLN25_05710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HSQ10094.1"
                     /translation="MKNDQPSRRGFFKAVAIGGATAAAAVATTLVGERKIISDTAVAD
                     DSKQLGYREPAHLRHDSRTTQVETGDSPMLTKKSAGAATAQGRLARAVSGMHGRT"
BASE COUNT          437 a          759 c          820 g          429 t
ORIGIN      
        1 cccggaggag gcgatcgact ttacctacca ggtcgatctc acgcgatgca aggcgcaccg
       61 tgcgtgtgtc aacgcgtgcg gagcgatcgg tgcgatcgat ttcagccgcc cggcgtcgaa
      121 tcgcacagag cagttcgatc tggtgttcga tttgtccgcg cagcccctgc tgaacatgcc
      181 cgagaagccg tttggctacc aggcgccagg cgctgatcct ctggagcagg cgcaggccgc
      241 gacgcgcctg gtgcagatgg tgggcgagtt cgaaaagccg cgctacgtgc agtacgacga
      301 gaagctttgc gcgcacagcc gaaatacaaa gaccggctgc acgcgctgcc tcgacgtctg
      361 cgccaccgac gccgtcagct cactgggcga caagatctgg gcacacccgt tcctgtgcat
      421 gggttgcggc gactgcgcaa ccacctgtcc gaccggtgcg ctgcactttg cctaccccgc
      481 cgtggccgac atcggcacgc gcctgcggaa gctcctggcg gtgtatcggg gggcgggtgg
      541 caaggacgcg gcgctgctct tccacaacgg cacgagcggt cgtgagcaga tcgcgcgatt
      601 ggcgcgtgtg ggcaaggggc tgccggcgcg cgtgctaccg atcgaggtgc acagcgtggc
      661 gacgatcggc atcgatgttg tgctgggcgc catcgccttg ggcgcgagcc aggtggtctc
      721 attgctcgac gacaccggtg cagacgaaca cggcgcgttg ctggatcagc aagcgcgcat
      781 cgccggtgcg atcctcaacg ggctcggata cagcggcacg cattggcaga ccttgcgagc
      841 cgaggatgcc gccgcgctgc aggcagcgct ctggtcatct cagccggcgg cgaccgtcgc
      901 cgagcccgcg acctttaacc tgtccaaccg caagcgcgag acgctcgact tcgccatcca
      961 tcacctcgtg caacaggcgt cggtcccgcc ggacgaaatc gcgctgcccg ccggctcgcc
     1021 gtttggcacg ctcgtcgtcg acgcgtcgcg gtgcaccctg tgcaaggcct gcatcggcgc
     1081 ttgcccgagt tccgcgctgc aggatgctga ggacgcaccg aagctgcgct tcatcgagcg
     1141 caattgtgtg cagtgcgggt tgtgcgagac gacctgtccc gagcaggcca tcagcctggt
     1201 gccgcgcctg ctggtgacac ccgcggcccg tcagcccgtc acactgaatg aagccgagcc
     1261 gttccattgc gtgcgctgcg gaacgccgtt cggtacgcgg caaatgatcg acgtgatgac
     1321 cggccgtttg ggcgcgcatt cgatgttcgc cggcgcggcg ctgcggcgta tccagatgtg
     1381 tgccgactgt cgcgtgatcg acatgatgga agcgaagaac gaagtctcga tcttcgacgt
     1441 caagcgatga gcgcgaccaa cgcagcgaag ggtgttgtcc tggagcccga ggacgaagga
     1501 cgcatgcagt tgtatgcgct gctcgcgcgc gtgttcgctg cgggaccgga cgaaggcctg
     1561 ctgcgggcgt tggcgcagtc agccgggagc gtcgagggtg atggcgaact ggcgcaggcg
     1621 tggaacgatc tggccgcggc ggcggcgcat acgtcggcgc gagatgcgac gctggagttc
     1681 gacgaaacct tcattggtgt aggcaaggct cccgtcagca cctacctgtc gcactaccat
     1741 acagccgcgc acaaggaagc gatgctggtc gaactgcgcg acacgctggt tcagctggga
     1801 ctggcgcgca cggttgcatc ggccgagccg gaagacaacc tcactgccgt tctggaagtc
     1861 atgcgtcatc tcgtcgccgc gggatccgat gagaagtcct tgcagcgtca gcgggagttt
     1921 tttgttcgtt atctgcagcc ggtgtaccgc gagttttgtg atgcggcgca caaggcagat
     1981 ctcagcgcct tctacaaggc agcggttcgt atcctcgaag tcttcatggt ttctgatctg
     2041 gcccagttcg agatgggcta gtaatcacta agggaacctg agtgttgacg gaccgttgac
     2101 acacggggga tgattgggtc gccaagggga atgaaggatt cacaaaatga aaaacgatca
     2161 acctagccgt cgcggcttct tcaaggctgt tgcgattggc ggcgcaactg cggcagcagc
     2221 cgtggcgacg actctcgtcg gtgagcgcaa gatcatcagc gataccgctg ttgccgatga
     2281 ctcgaagcaa ctcggctatc gcgaaccggc gcacctccgg cacgactccc gcaccaccca
     2341 ggtcgagacg ggagactccc cgatgctcac gaagaaatcc gcgggggccg cgacggccca
     2401 gggccgcctt gcgcgcgctg tttccggcat gcacggccgg acgat
//
LOCUS       DATIRT010000247         1558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1413599, whole genome shotgun sequence.
ACCESSION   DATIRT010000247 DATIRT010000000
VERSION     DATIRT010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413599"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..>1558
                     /locus_tag="VLN25_05715"
     CDS             207..>1558
                     /locus_tag="VLN25_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HSQ10095.1"
                     /translation="MTATSTSATEARLREVLARRILILDGAMGTMIQHYKLPEAGFRG
                     ERFAGHPVDLRGNNDLLVLTRPEIVRDIHEQYLDAGADIIETNTFGATSIAQDDYRLG
                     HIAYEMNVAAARLAVEAAAKFTTPDWPRYVAGALGPTPKTASISPDVNDPGARNVSFD
                     QLVAAYGEQARGLLDGGVDLLLIETIFDTLNAKAAIFAVEAEFERRGERGLPRVPVMI
                     SGTVTDASGRILSGQTVEAFWHSVRHARPLTVGLNCALGAALMRPYIEELAKIADTYV
                     SVYPNAGLPNPMSDTGFDETPEITSSLLREFADAGFVNIAGGCCGTTPEHIRAIAQEI
                     EGLTPRAVPSSDHTMKLSGLEPVKVGDESLFVNIGERTNVTGSKAFARLILNEQYEEA
                     VSVARQQVENGAQVIDINMDEAMLDSAAAMTRFLNLIASEPDIARVPVMIDSSKWSVI
                     "
BASE COUNT          283 a          524 c          503 g          248 t
ORIGIN      
        1 caaagcagac gccgccgtgc ggcccaaccc tgcgggaagg ccagtggcgg gcgcattgcg
       61 tcgttaccgc ttgctgattg gcatcagcca acggcgctcg cggcgcctcg cactgcctcc
      121 cgccattggc cttcgcactc cgggctttag cgtgaacagg ccctaaaatg ccaggctccg
      181 ccaggaagtt tttcgccgtc cccctgatga ccgccacttc gacgtccgcc accgaggccc
      241 gccttcgcga ggtcctcgcg cgccgcatcc tgatcctgga cggcgcgatg gggaccatga
      301 tccagcacta caagttgccg gaggccggct ttcggggtga acgcttcgcc ggtcatccgg
      361 tggacctcag gggcaacaac gacctgctcg tgctgacgcg accggagatc gttcgcgaca
      421 ttcacgagca gtacctggac gccggtgccg acatcatcga gaccaacacg tttggtgcaa
      481 cgtccattgc ccaggatgac taccggctgg ggcacatcgc ctacgagatg aacgtcgccg
      541 cggcgcggct cgcggtcgag gccgcagcaa agttcacgac cccggactgg ccgcgctatg
      601 tcgcgggtgc gctgggaccc actccgaaga ccgcgtcgat ctcgcccgac gtcaacgatc
      661 ccggcgcgcg caacgtgtcc ttcgaccaac tcgtcgccgc ctatggcgaa caggcacgtg
      721 gtctgctcga cggcggagtc gacctgctgc tgatcgaaac gatattcgac acgctcaacg
      781 ccaaggctgc gatcttcgcc gtcgaagcgg agttcgagcg gcgcggcgag aggggcctgc
      841 cgagggtgcc cgtgatgatc agcggcaccg tgaccgatgc gtcgggccgg atcctgtcgg
      901 gccagaccgt cgaggcgttc tggcactccg tccgtcacgc gcgaccgctc acggtcgggc
      961 tcaattgcgc gttgggtgcc gcgctgatgc ggccgtatat cgaggaactc gcgaagatcg
     1021 ccgacaccta cgtcagcgtg tacccgaacg cgggcctgcc gaacccgatg agcgacaccg
     1081 gcttcgacga gacgccggag atcacgtcga gcctgctgcg cgagttcgcc gatgcgggct
     1141 tcgtcaacat cgccggaggc tgctgcggca cgacgccgga acacatacga gcgatcgcac
     1201 aggagattga aggtctgacg ccgcgcgcgg taccctcgtc ggaccacacg atgaagctct
     1261 cggggctcga gccggtgaaa gtcggcgacg agagcctgtt cgtcaacatc ggcgagcgca
     1321 ccaacgtcac gggctcgaag gcgttcgcgc gcctcattct caacgagcag tatgaagagg
     1381 cggtcagcgt cgcccgccag caggtcgaaa acggtgcgca ggtcatcgac atcaacatgg
     1441 acgaggcgat gctcgactcg gccgccgcga tgacgcgctt cctgaacctg atcgccagcg
     1501 agccggatat cgcgcgcgtg ccggtgatga tcgacagctc caagtggagc gtcatcga
//
LOCUS       DATIRT010000248         3573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1511999, whole genome shotgun sequence.
ACCESSION   DATIRT010000248 DATIRT010000000
VERSION     DATIRT010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511999"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="VLN25_05720"
     CDS             <1..617
                     /locus_tag="VLN25_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HSQ10096.1"
                     /translation="RRGADPARVLIKIAATWEGIRAAEVLEREGVHCNITLLFSTAQA
                     LAAAQAGATLISPFVGRIYDWHKKSAGTGWDEAAMRGDNDPGVRSVRTIHAQLKDGGY
                     PTEIMGASFRNVGQILALSGCDLLTISPELLDELASVEGDVELKLTAPANSGASSGRG
                     APLQEAEFRFALNDDAMAVEKLAEGIRVFAADTRRLEALIEAMA"
     gene            621..2228
                     /locus_tag="VLN25_05725"
     CDS             621..2228
                     /locus_tag="VLN25_05725"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HSQ10097.1"
                     /translation="MLILPYQTRFTAKSLPVVTLALAVVNLLVYLVLQSGDNAAYQRA
                     ADYYFSTTLPQVEMPRYAAYLERRNDRKAMQVLRAIRSGPSDDEARAVLMVMQHDREF
                     MKDLKAGLVVRSDEPGNAAWREQRDRFHGLMSQVFTQRFALEPGSADLVRLFTYQFLH
                     GDAMHLLGNLAVLLLAGPFAEAALGRARFLLAYLASGVIAGAVHLVVSDQGLIGASGA
                     IAGTMAMVAVLYGTRKVPVFYWLFVYFNTARIPALALLPVWLVIEAMQWALSSGTRVA
                     YDAHIAGFVAGATFAWLLKPADDGKVDRILDEQYAEAPAERKSTLLTEARQAAAKLDT
                     RRAARAYSELLQEDPTNTQYATAYFNMALLGRDKDTLADAALRALWIRTRHARTELRA
                     VYTQMSQPHVLAVLPVDEQLRLARRLVATREDAAALRVLDGLLGHDTTKNLYGRQLAD
                     CLLGLFTTYSRHGLRQPAEQVRQRLSQYFPSPGTLGGLAPNREPPMTIRGATTRGTTP
                     RGNPATVPASDLELDLDTRLHTRWGPD"
     gene            complement(2256..>3573)
                     /locus_tag="VLN25_05730"
     CDS             complement(2256..>3573)
                     /locus_tag="VLN25_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10098.1"
                     /translation="FFLLPLDRSVLLRIGGLSAALVVSLVLLLFGPFGTVLIAAAAFA
                     LLVVGARYGFKIIERSSKGYLIPSDYPLTDEDLVGPYLPYKYVAMNMVAGAVAGLFIT
                     ILGGHEFLGILIWGFFFVVLMPAATMRLVMTGSLRGALNPTELVNLIQRVGKPYAALC
                     AFGFFADLSRSYGIVFLASAAGVGAGGAAGMGILAVMLVFLLSAAFWYFSYVICALIG
                     YAMYQYADRLEISVVGPGEERLMSSTGRHADVKSRTRDALIGQMVSAGEIKEAIDLLQ
                     DELRERPSDLSLHARLHTLLLAENYTPRIEDHTEKYLALLVRSLNWREALDLVEEALA
                     RRADWVPRPADAIAPLARAALQKGRPQLAATLIRGFDKKHPNHPDIPQIYLVGAQLMA
                     ETAHNPDEARRILKYLLQRHGSDPVAAEAGRYLEVMNKVAEGARDR"
BASE COUNT          633 a         1194 c         1174 g          572 t
ORIGIN      
        1 cgcggcgcgg agccgaccct gcacgcgtgc tgatcaagat cgcggcgacc tgggagggca
       61 tacgcgccgc cgaggtcctc gagcgcgagg gcgtccactg caacatcacg ctgctcttct
      121 cgacggccca ggccctggct gccgcacagg cgggcgcgac cctgatttcc ccgttcgtcg
      181 gtcgcatcta tgactggcat aagaagagtg cgggcacagg ctgggacgag gcggcgatgc
      241 gcggcgacaa cgacccgggc gttcgatcgg tgcggacaat tcatgcgcaa ctcaaggacg
      301 gcgggtaccc taccgagatc atgggcgcga gcttccgcaa tgtcggacag atcctggcat
      361 tgtcgggatg cgacctgctg acgatcagcc cggaactgct ggacgaactg gcctccgtcg
      421 aaggcgacgt cgaactcaag ctcactgcgc cggcaaattc cggagcaagt tccggacgag
      481 gagcgccgct gcaggaggcc gagttccgct ttgcgctcaa cgacgatgcc atggcggtgg
      541 agaagctcgc cgaaggcata cgcgtcttcg ccgctgatac gcggcgactc gaggcactga
      601 tcgaagcgat ggcctgatcc atgctcatcc ttccgtacca gacccgcttc accgccaagt
      661 cgttgccagt ggtgacgctt gcgctcgcag ttgtcaacct gctcgtctat ctggtcctgc
      721 agtcgggcga caacgcggcc taccagcgcg ccgcggacta ctacttcagc accacgttgc
      781 cgcaggtcga gatgccgcgc tacgccgcct atctcgaaag gcgcaacgac cgcaaggcga
      841 tgcaggtgct gcgcgcgatc cggtccggac cgagtgacga cgaagcgcgc gcggtgctca
      901 tggtcatgca gcacgaccgc gagttcatga aggacctgaa ggcgggcctg gtcgttcgct
      961 ccgacgagcc cggcaatgcg gcctggcgcg aacagcgcga ccgcttccac ggcctgatgt
     1021 cgcaggtgtt cacgcagcga ttcgcgctcg aaccgggatc cgccgacctg gtgcgccttt
     1081 tcacctacca gttcctgcat ggcgacgcga tgcacctgct gggcaacctg gcggtgttgc
     1141 tgctggccgg tccgttcgcc gaagcggccc tgggacgcgc ccgatttctt cttgcctacc
     1201 tcgccagcgg cgtgatcgct ggcgcagtgc atctcgtggt cagcgaccag ggcctgatcg
     1261 gcgcatcggg cgccattgcc ggcacgatgg cgatggtcgc cgtcctgtac ggcacccgga
     1321 aggtgccggt cttctactgg ctgttcgtct acttcaacac cgcacgcatt ccggcgctgg
     1381 cgctgctgcc ggtgtggctg gtgatcgagg cgatgcagtg ggcgctgtcg tccggcacgc
     1441 gggtggccta tgacgctcac atcgccggct tcgtcgccgg ggccaccttc gcgtggctgt
     1501 tgaagccggc tgacgacggc aaggtcgacc gcattctcga cgagcagtac gccgaagcgc
     1561 ctgcggaacg caagtcgacg ctgctgaccg aggcacgcca ggcggccgcc aagctggaca
     1621 cccggcgcgc ggcacgcgcc tattcagaac tgctgcagga agacccgacc aacacgcagt
     1681 acgccactgc gtacttcaac atggcactgc tgggtcgcga caaggacacg ctggccgatg
     1741 cggcgctgcg tgcgctctgg atccggacac ggcatgcgcg caccgagctg cgggcggtgt
     1801 acacgcagat gagccagccg cacgtgcttg ccgtactgcc ggtcgacgag cagctgcggc
     1861 tggcgcgtcg tctcgtcgcc acgcgcgagg atgccgctgc gctacgcgta ctcgacgggc
     1921 tgctgggcca tgacaccacc aagaacctct acggacggca actggcggat tgcctgctgg
     1981 ggctctttac aacgtattcg cggcacggac tgcggcagcc ggccgaacag gttaggcagc
     2041 gcctgtcgca gtacttccca tctccgggaa ccctgggcgg gctggcaccg aatcgcgagc
     2101 cgccgatgac gatccgtggc gcgacgaccc gcggtaccac gccgcgcggc aatcccgcta
     2161 ccgttccggc gtccgatctc gaactcgatc tcgacacgcg cctgcacacc cgatgggggc
     2221 cggactagcc ggggctccag tctgaggcga tcgcatcagc gatcgcgcgc accctcggcg
     2281 actttgttca tcacttcgag gtagcgcccg gcttcagcgg caaccggatc gctgccatgc
     2341 cgctgcagca ggtacttcag gatgcggcgc gcctcgtcgg gattgtgtgc ggtctcggcc
     2401 atcagttgcg cgcccacgag atagatctgt gggatgtccg gatggttcgg gtgcttcttg
     2461 tcgaagccgc ggatcagcgt ggccgcgagc tgtggccggc ccttctgcag cgccgcgcgc
     2521 gccagcggag caatcgcgtc cgccggtctc ggcacccagt ccgcgcgccg ggcgagagct
     2581 tcctccacca ggtcgagcgc ttcgcgccag ttcagcgacc tgaccagcag cgccaggtac
     2641 ttttcggtgt ggtcctcgat cctgggcgtg tagttctccg ccagcagcag cgtatgcagg
     2701 cgggcgtgca gcgacagatc gctcggccgc tcgcgcagct cgtcctgcag caggtcaatg
     2761 gcttccttga tctcgcccgc actcaccatc tgcccgatca acgcgtcgcg cgtacgcgac
     2821 ttgacgtcgg cgtggcgccc ggtcgaactc atcagccgct cttcgcccgg gccgacgacg
     2881 gaaatctcga ggcggtccgc atactggtac atcgcatagc cgatcagcgc acagatgacg
     2941 tagctgaagt accagaaggc ggccgacagc aggaagacca gcatcaccgc gaggattccc
     3001 atcccggccg cgccgcccgc accgacgccc gcggcgctgg cgagaaaaac gatcccatag
     3061 gagcgcgaca ggtcggcgaa gaacccgaac gcgcacaacg ccgcatacgg cttcccgacg
     3121 cgctggatca ggttgaccag ttccgtggga ttgagtgcgc cacgcaggct gccggtcatg
     3181 acgaggcgca tggtggcggc gggcatcagg accacgaaga aaaagcccca gatcaggatg
     3241 cccaggaact catgtccgcc gaggatcgtg atgaagaggc cagccaccgc accggccacc
     3301 atgttcatcg ccacgtactt gtacggcagg tacgggccga ccaggtcctc gtctgtcagc
     3361 ggatagtcgg agggaatgag atagcccttg gacgaccgct cgatgatctt gaagccatag
     3421 cgcgcgccga cgacgagcag cgcgaatgcg gcagcggcaa tcagtaccgt gccgaacgga
     3481 ccgaagagga gcagtaccag cgagacgacc agcgccgctg acagcccgcc gatgcggagc
     3541 aggacggaac ggtcgagcgg tagcagaaag aat
//
LOCUS       DATIRT010000249         6807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1807324, whole genome shotgun sequence.
ACCESSION   DATIRT010000249 DATIRT010000000
VERSION     DATIRT010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6807
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1807324"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VLN25_05735"
     CDS             complement(<1..334)
                     /locus_tag="VLN25_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HSQ10099.1"
                     /translation="MTLAEWSLVVGALLVTMVLAGSLLGRLPLSSAMVYLALGWLLGP
                     DVANVLRPDPYLHSGVLERLAEVALLISLFAVGLRLGVPLRDRRWRLPLRLAFVSMAI
                     MVALVASIG"
     gene            complement(369..1295)
                     /locus_tag="VLN25_05740"
     CDS             complement(369..1295)
                     /locus_tag="VLN25_05740"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HSQ10100.1"
                     /translation="MGHADRATAAKTGRFVEGTLVDGQSAEHHPRKYMLYEPASRGHA
                     PAPLLVLLHGCKQGAAEFAAGTRMNEAAEAAGVVVLYPQQSLGANLLRCWNWYALHDR
                     SNKDGDAAVIAAMTRQVMREHDIDATRVYVAGMSAGGGMAAVLVRDYPELYAALGVHS
                     GVPAGLAHDVYSAMRLMSTGPVPSEAEAGPGTGNVGGASAASIVFHGDEDTVVHLSNG
                     VAIHGGAGGSQVPPLLDTQQSTTQPRPGHRGFTRSVEYGAGGVTERELWIVHGAGHAW
                     AGGSSEQPDTDSSGPDASREMLRFFLQHQLSA"
     gene            complement(1736..2113)
                     /locus_tag="VLN25_05745"
     CDS             complement(1736..2113)
                     /locus_tag="VLN25_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10101.1"
                     /translation="MNNITQPFINLTTANAELITRFAQSPEVAELANTGAQKYFELAQ
                     KSFGRMAASDAYANLVRRLSENYSNFAHEYSESLMGLAAAGQNLMAQHLEATSSRLEE
                     SGKAAVAVIEKASDRPTKSRSKR"
     gene            complement(2452..3069)
                     /locus_tag="VLN25_05750"
     CDS             complement(2452..3069)
                     /locus_tag="VLN25_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009454498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HSQ10102.1"
                     /translation="MYHGEKFNAVSHLVGTVLALAGAIVLIVLASMEGDPWKVVSVSI
                     YGATLVVMYSSSTLYHSARGRAKDVLRKMDHQSIYLLIAGSYTPFCLVTLRGPWGWSL
                     FGVVWGLALLGGLQELRPRNDARILSVVIYLLMGWVALVALIPLVYALGRAGFAWVAA
                     GGLFYTIGIVFYALDTRLTHAHGVWHLFVLAGSATHYVAINRYVI"
     gene            3481..4302
                     /locus_tag="VLN25_05755"
     CDS             3481..4302
                     /locus_tag="VLN25_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10103.1"
                     /translation="MNMRHGLRNGLLVLGLVIGSVTSATAQVSIGIGLPGVSIGINMP
                     VYPHLVPVPGYPVYYAPRVDSNYFFYDGMYWVYQSDNWYASSWYNGPWGLVAPDVVPL
                     YVLRVPVRYYRRPPPYFHGWKADAPPRWDDHWGKDWKQRRGDWDHWDRRSAPPRAPLP
                     TYQKPYSGNRYPHPEQQPVLQGQKYKYQPHDPVVQQHYQVQRTQGGPSNAMQGGSNAR
                     RQEQSQQGAPRHEQQSPQSAGQDRGSQGKGSAPQSAQGQDKGGGNSGQQGGENRK"
     gene            4630..5802
                     /locus_tag="VLN25_05760"
     CDS             4630..5802
                     /locus_tag="VLN25_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HSQ10104.1"
                     /translation="MNAESVAAPEQLSPNTAESHLTLPATQLPGEARGLAIGILASLA
                     TVFAVSWAQDFLVPLLLGIVIAYTLNPLVEWLEAIRIPRTAGTVVVMAGVIAALALGT
                     YSLRGQMQTIIEQLPEAATQFSTALARFRSSQNDTLQKIQNAATEVETATTRAVSGVA
                     PPRKPATQVTADLPKFRLNDYLWAGSMGAVGAIGQAAMVIFLVFFLLLGGDTFKRKLV
                     RVTGPSLSKRKITVKILDDINGSIQKYMLMLLATNLLVVLLCWIAFYFIGLENAGAWA
                     VAAGIFHIVPYLGPAIAAGAIAMAAFMQFDSFSMALLTGSAVVAIATLVGTFVTTWMT
                     GRIANMNSAAVFVSLLFWGWLWGIWGMLLSIPIIVIVKVVCQHVEQLQPLAELLGE"
     gene            5929..6609
                     /locus_tag="VLN25_05765"
     CDS             5929..6609
                     /locus_tag="VLN25_05765"
                     /inference="COORDINATES: protein motif:HMM:NF018745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HSQ10105.1"
                     /translation="MSRSNIGKSAWIIRIGAMQRLAVVTAVGIAAYLAQPESISWHTR
                     AVVTWDLSVLVYLCLAWWLIASTDAGMTRGHALSQDQSGFVIFLFVVVAAFASVVAIG
                     FVVSTIRELDPWSKAWHLALTIGALISSWLLIQTVFAFHYARRYYAILHREHTATPEL
                     QFPGAREPDYLDFAYYSFVVGMTSQVSDVAVTARDMRRLTLVHSVLAFVFNIAVLALS
                     INIIASVI"
BASE COUNT         1327 a         2139 c         2044 g         1297 t
ORIGIN      
        1 caccgatgga ggcgaccagc gccaccatga ttgccatcga gacgaacgcc aggcgcagcg
       61 gtagccgcca acgacggtcg cgcaggggga cgccgagccg cagaccgaca gcaaaaagtg
      121 aaatcagcaa tgcaacctcg gccagtcgtt cgagaacgcc ggaatgcaga tacggatcgg
      181 gacgcagaac gtttgccaca tccggaccca ggagccagcc cagcgccaga tagaccatcg
      241 cgctgctcag cggcagccgc cccagcagac tccctgccag caccatcgtg accagcagcg
      301 cgccgacgac cagggaccac tcggccaatg tcactgctgc agctccatgg tgctcgaact
      361 atgcgcttct atgcgctgag ttgatgttga aggaagaacc gcaacatctc gcgcgatgcg
      421 tccggaccac tggaatccgt gtcgggttgc tcgctgctgc cacctgccca ggcgtgtccg
      481 gcgccgtgaa cgatccacag ctcgcgctcc gtcacgccac ccgcaccgta ctcgaccgat
      541 cgggtgaatc cacgatgtcc cggccgcggc tgcgtcgtcg actgctgcgt gtcgagcaga
      601 ggaggaacct gtgaaccgcc tgcacccccg tgtatggcca ctccgttgga aagatgaacg
      661 accgtgtctt catcgccgtg gaagacgatg ctggccgcgc tcgcgccacc gacgttacct
      721 gtgcctggcc ccgcttccgc ctcggaggga acgggtccgg tcgacatcag tcgcatcgcc
      781 gaatagacgt catgtgcgag tccggcaggg actcccgaat gaacgcccag cgcggcatac
      841 agttccggat agtcccgcac cagcacggca gccatcccgc cgcccgccga catgcccgcc
      901 acgtagacgc gggtcgcgtc gatgtcatgc tctcgcatca cctggcgggt catggcggcg
      961 atcaccgcag cgtcgccgtc cttgttcgat cggtcgtgga gcgcatacca gttccagcag
     1021 cgcaagagat tggccccgag ggactgctgg ggatacaaca cgaccacccc cgcagcttca
     1081 gccgcttcgt tcatgcgagt gccggccgcg aattcggcgg cgccctgctt gcatccgtgc
     1141 aggagcacga gcagcggtgc cggagcgtgg ccgcgactgg ccggctcgta cagcatgtac
     1201 tttctcgggt ggtgctcggc agactgtccg tcgacgagag tgccctcgac gaaacgtccg
     1261 gtcttcgcag cggtggcccg atccgcatgt cccaagggga cggccagagc cggacgtttc
     1321 tcgtttcttg aagcggatcg gcgcagagct tgcagcaacg ctccgcctgc ctggacaatt
     1381 cgcgctttct gcttcgtcca gcgagcgcgc caaagggccg cacccacgcc acgctttgca
     1441 aggacggcct ggcgaagtag aagttgcggc tcaagccgtg gcaaccggtc agcctctcgc
     1501 gcaagatggc tttcgagctc agcgagcttg attttgtagg cctcgctgtc ggggtcaatt
     1561 gaagcgagct ccaggacgac gccgtgaatg actaggcgct gctgtgcctc agagtttgat
     1621 gggttggaag tcattgggtg tcctgggcgt cagtcgagcg tgtcgacgaa aaggaaaacg
     1681 ccgcctccgc ggcggcgcga cttgtctggc tcgtccaaag gaagtgagcc cgcgactatc
     1741 gttttgatcg ggacttggtc ggcctgtccg acgccttttc gatgacggcg accgccgcct
     1801 tcccggactc ttcaaggcgg ctcgacgttg cttcgaggtg ttgcgccatc agattctgtc
     1861 cggctgcagc gagtcccatg agactttcgg agtactcatg agcaaagttt gaatagtttt
     1921 cgctcagcct gcgcaccaga ttggcgtaag catcggaagc cgccattcgg ccaaacgact
     1981 tctgcgcgag ttcaaaatat ttctgagcac cggtgttggc gagttcagcc acctccggcg
     2041 actgcgcaaa gcgtgtgatg agttcagcgt tggcagtagt gaggttgatg aagggttgcg
     2101 tgatgttgtt catttcgtag accttgtccc cggcttgcgc cggactggga agccacttca
     2161 aagacggcgg tcaacctgca gcttcgatag cggagcgccg tatcgggcac gaccctgatt
     2221 ttgtcgtgcg cacgagttcg taaacgagtt cgcgagcccc tgtcttggga caccgtgaga
     2281 caccatgttg actgttcgtg gcgatgacat aggcaccgac gaggcgcctg cgggacgcct
     2341 tgatcaccac caatagtcag cggcgtaggc gcacgaactg acgcagtgtg gctgtgggtt
     2401 tgaatggtgt cggtgcgtta ccgtacgcaa cgccccctcg cgggcggcac ttcaaatgac
     2461 gtacctgttg atcgcaacgt agtgtgttgc gctgccggcc agcacaaaca agtgccagac
     2521 accgtgtgca tgggtcaggc gagtgtcgag ggcatagaag acgatgccaa tggtgtaaaa
     2581 gagcccgccc gccgcgaccc aggcgaatcc ggcgcggccc agggcataaa ccagcgggat
     2641 cagggccacg agagcgaccc agcccatcag caggtagatc accactgaga gaatgcgcgc
     2701 gtcattccgt ggccgaagtt cctgcaaacc accgagcagg gcaagccccc acacgacccc
     2761 gaacaatgac cagccccagg ggccacgcag tgtcaccaga cagaacgggg tgtaactgcc
     2821 ggcaatgagc aggtagatgc tctgatgatc catctttcgc agaacgtcct tggcccgccc
     2881 gcgagcgctg tgatacagcg ttgatgagct gtacatgacc acgagggtcg cgccgtagat
     2941 cgagacgctg accactttcc acggatcacc ctccatcgaa gccagcacaa tcagcacgat
     3001 cgcgcccgcg agcgccagta ccgtcccaac caggtgggac actgcgttga atttttcgcc
     3061 gtggtacatg ccgtagacat ccgtgctgcc ggtcgacgct gtagggatcg tagcttccgt
     3121 tgccatgcat acaaaacggc gtgaccgttc gatcacgccg cttgcgtgtt gcactgacga
     3181 cggatgtcga cccgttgctg accagcactc ctactcgact gtcgcgtcgg tgggtcgccc
     3241 aaggcgcgcc tggagccggg ccgcggggtc gtgaccgctt cgcatttccg tcatgacccg
     3301 ttgtgagctc catctcaagc gatgtgtgcg ctgccgcacc gacgacaacg aatgctgcgc
     3361 ttagcgtgcg agttcagagt tcgccggtcg cgcctagaaa tcggttcctg gcagcgtgcc
     3421 acaccgcttt ccttcgcctt gagtcagaga tgtctcgaac ccggtgattc aaccgagagc
     3481 atgaatatga gacacggatt gcgtaacgga ctcctggtgc tggggttggt gatcggatcg
     3541 gtcacctcgg cgacggccca ggtgagcatc ggcatcggcc tgccaggtgt ctccatcggg
     3601 atcaacatgc ccgtttaccc gcatctcgtt ccagtgccgg gataccccgt ctactacgcg
     3661 ccacgagtgg actccaacta cttcttctac gacggcatgt actgggtcta ccagtcggac
     3721 aactggtacg cgagctcctg gtacaacgga ccgtggggac tcgtcgcccc ggacgtcgtt
     3781 ccgctgtacg tactgcgtgt cccggtgcgt tactaccgcc gaccaccgcc gtacttccat
     3841 ggatggaagg ccgatgcgcc cccgcgctgg gatgatcact ggggcaagga ttggaaacaa
     3901 cgccgaggcg attgggacca ctgggatcgt cgttcggcac ccccccgtgc tccgctgccc
     3961 acctatcaga agccgtattc cgggaatcga tatccgcacc cggagcagca gcctgtactt
     4021 caggggcaga agtacaaata ccagccgcat gaccccgtcg tgcagcagca ctatcaagtg
     4081 cagcggacgc aagggggtcc ttcgaatgca atgcagggcg gatccaacgc tcgccggcag
     4141 gagcagtcgc agcagggagc accccgtcac gaacagcagt cgcctcaatc cgcgggtcag
     4201 gacagagggt cccagggaaa gggatcggcg cctcagtccg cccagggaca ggacaagggc
     4261 ggcggcaata gcggacagca aggcggagag aacaggaagt aacgtccgga cggcagcaac
     4321 gctctcgtcc cgcaatgaag cgccacttca ccggcgcatt acgagagttg catgccagtc
     4381 cgacaacgac atctcaacgg aggctcacct atcggatgtc gccagcccgc gccaggtgac
     4441 aggtaactca tctaccgcaa tcccacaatc ggaaagcaca ccatgcacaa ggcttcatca
     4501 ctgaccgacg gcagatcgtc cacaaagtcg caaatgagcg accgagcccc gccgtcccgc
     4561 cgcaatggat cccggtcaaa cgtcgtttga cgacgcgcca tgtccaacgc tgtcacctcc
     4621 gcctcaatcg tgaacgctga gtcagtggct gcaccagagc aactcagccc caacaccgcc
     4681 gagtcgcact tgacgttacc ggccacgcaa ttgccgggag aggcgcgcgg cctggcgatc
     4741 gggatactgg cgagtctggc caccgtgttt gccgtgtcgt gggcgcaaga ttttctggtg
     4801 ccgttgctgc tcggcatcgt cattgcctac acattgaacc ctctcgtcga atggctcgaa
     4861 gcgatcagga tcccgcgcac tgccggaact gtcgtcgtca tggcaggcgt gatcgccgcg
     4921 ctggctctag gcacctactc gctgcgcgga cagatgcaga ccatcatcga gcaactgccc
     4981 gaagcagcga cccagttctc cacggcactt gctcgctttc gcagcagcca gaacgacacc
     5041 ctgcagaaga ttcagaacgc cgccaccgag gtggaaaccg cgacgactcg tgcggtcagc
     5101 ggcgtcgcgc ccccccgcaa acccgccact caagtcaccg cggacctgcc aaagttcaga
     5161 cttaacgact acctgtgggc cggttcgatg ggcgcggtcg gagccattgg acaggcagcg
     5221 atggtcatct tcctggtgtt cttcctgctg ctcggtggcg ataccttcaa gcggaagctg
     5281 gtgcgggtca cggggccatc actgtcgaag agaaagatca cggtcaaaat cctcgacgac
     5341 atcaacggct cgatccagaa gtacatgctg atgctgctgg cgacaaatct gctggttgtc
     5401 ctgctgtgct ggatcgcgtt ctactttatc ggactcgaga atgccggggc ctgggcggtg
     5461 gcggcaggca ttttccacat cgttccttat ctcggtcctg cgattgcggc cggtgccatc
     5521 gcgatggccg ccttcatgca attcgattcg ttttccatgg cgctgctgac cggaagcgcc
     5581 gttgtggcga tcgctacact ggtcggaact tttgtcacca cctggatgac agggcgaatc
     5641 gccaacatga actcggctgc ggtcttcgtt tcactgctgt tctggggctg gctgtggggc
     5701 atctggggca tgctgctcag cattccgatc atcgtgatcg tcaaggtcgt gtgtcagcac
     5761 gttgaacagc tgcagccgct agccgaactc ctgggtgagt gagtcagacg gcgcagcaga
     5821 gggggcattg ttcgcgacgg ctgccatcga tcagcgaact gactgcagac tgaagtcctg
     5881 gatgtgcgct gctcaccgaa tcaatgcggg tacgatttcg ctaccccaat gagcagatca
     5941 aatatcggca agtcggcgtg gatcatccgc attggcgcca tgcagcgcct tgcggtagtc
     6001 actgccgtcg gtatcgccgc ctacctcgcg cagccggaat cgatttcgtg gcacacgcgc
     6061 gcagtcgtga cctgggacct gagcgtgctg gtctatctct gtcttgcctg gtggctcatc
     6121 gccagcaccg acgccgggat gacgcgtggc cacgcgttga gccaggatca gagcggcttt
     6181 gtcatcttcc tgttcgtcgt cgttgccgcc ttcgccagtg tcgtggcgat cgggtttgtc
     6241 gtcagcacca ttcgggaact cgacccctgg tccaaggcgt ggcatctcgc cctgactatc
     6301 ggtgccttga tctcttcatg gctgctcatt cagaccgtct ttgcgtttca ctacgctcgc
     6361 cgctactacg ccatcctgca ccgggaacac acggcaacac cggagctgca atttccagga
     6421 gccagagagc ccgactacct ggactttgcc tactactcgt tcgtggtcgg aatgacctca
     6481 caggtctccg acgtcgccgt gacagcacga gacatgcgca ggctcacgct ggttcacagc
     6541 gtgctggcct tcgtgttcaa catcgcggtg ctggctctca gcatcaatat catcgccagc
     6601 gtgatctagg cctttctttc gacctcgttt caagatcgag tcaacgtaac taggtcaaga
     6661 cgttcgatca ggaggcccta tgtgcgccag cgcacagacc gtcggccctg ctggggctat
     6721 tctgggttga cggtttccag ccgcaaggcg ctcgcgctta aaggcaactg gaatagttca
     6781 aagggcaggt gtagctcaag gcgttcg
//
LOCUS       DATIRT010000250         2373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_922180, whole genome shotgun sequence.
ACCESSION   DATIRT010000250 DATIRT010000000
VERSION     DATIRT010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922180"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="VLN25_05770"
     CDS             <1..1020
                     /locus_tag="VLN25_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10106.1"
                     /translation="AWASGVRAQLAEADGDQEEQMNLLRDVHHAQVFRLLVADLDGRL
                     TVERLADHLSALADATLALTLACAWKTVARRHRDIPTFAVIAYGKLGGKELGYASDLD
                     LIFLYDDPHADAPEIYSTLARRLVTWLSSQTSSGILFDIDLRLRPNGNAGLMVSSFEA
                     FSRYQRNEDGLGAWVWETQALTRARFSCGDAALGQRFEEERAWILSRERELPTLRADV
                     LAMRRKMLDGHPNPSALFDLKHDRGGMVDIEFIVQCLVLAHAHARPELVRNAGNIALL
                     LMAGAMGLIDSSLAHRVADAYRTFRLIQHRLRLNGAERARVEPEEVAGDADAVRRLWH
                     QVLES"
     gene            1027..2223
                     /locus_tag="VLN25_05775"
     CDS             1027..2223
                     /locus_tag="VLN25_05775"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSQ10107.1"
                     /translation="MRATLRALPSTVWLLGAISLLNDAASELVYPLIPLYLATVLSAG
                     PRALGFIEGAAEATAALLKLVSGVWYDRVRRAKGFVVVGYGLAAVARPLIAVVTAWPG
                     LLLLRVLDRLGKGLRSSPRDALLAHSVPAERRGLAFGLHRAFDNAGAVIGPLLAAALL
                     ALHVPIRDILLWAALPGALCVALALSIREPLNLVVPHARRAEWAWRTLPFAFRRMLVA
                     LALFTLGQASNAFILLRATELGMSATQVALLWAVVSATSTLLAVPLSAWSDRIGRVPL
                     LVGGWTLHALLFVALALVSAPALLWGIAVLLGIYMASTEGAERALIADLVGPDSLGTA
                     YGWYYLTKGLLLLPASAAFGWIWFARDAASAFLTAAALVATATIVLALWVLPAVNAHR
                     RAVRRG"
BASE COUNT          318 a          804 c          811 g          440 t
ORIGIN      
        1 gcctgggcga gcggcgtgcg cgcgcagctg gcagaggccg acggcgacca ggaagagcag
       61 atgaacctgc tgcgcgacgt acatcacgcg caggtgttcc gtctgctggt ggctgatctg
      121 gatgggcggc tgacggtcga gaggcttgcc gaccacctgt cggcgctggc agacgccacg
      181 ctggcactca cgctcgcatg cgcatggaaa accgtggcgc ggcggcaccg cgacattccc
      241 acgttcgccg tcatcgccta cggcaagctc ggcggcaaag aacttggcta cgcctccgac
      301 ctcgacctga tcttcctgta cgacgacccc catgccgatg caccggaaat ctattcgacg
      361 ctcgcgcggc ggctggtcac gtggctgagc tcgcagacct cgtccggcat cctgttcgac
      421 atcgacctgc ggctgcgccc caacggcaat gccggcctga tggtgtcgtc gttcgaggcc
      481 ttctcgcgct accagcgcaa tgaggacggg ctcggcgcct gggtctggga aacgcaggcg
      541 ctgacacgcg cgcgcttctc gtgcggcgat gctgcgcttg gccagcgttt cgaggaggag
      601 cgcgcctgga tcctgtcgcg cgagcgcgag ttgccaacac tgcgcgccga cgtcctggcg
      661 atgcgcagga agatgctcga cggccatccc aacccgagcg ctctattcga tctcaagcat
      721 gaccgcggcg gcatggtcga catcgaattt atcgtgcagt gcctggtgct ggcgcatgca
      781 catgcgcgac ccgaactggt ccgcaacgcc ggcaacattg cgctgctgct gatggccggc
      841 gcgatgggtc tcatcgattc gtcactggcc catcgggtgg ccgacgccta ccggacgttc
      901 cggctgatcc agcatcgcct gcggctcaac ggcgccgagc gtgcgcgcgt cgaacccgag
      961 gaggttgcgg gcgacgccga cgccgtacgc cgcctctggc atcaggttct cgaaagttag
     1021 gtcggcatgc gggcgacact ccgggcgctg ccttcgaccg tctggctgct cggagcgatc
     1081 agcctgctga acgatgcggc gtcggaactc gtctatccac tgatcccgct gtacctggcg
     1141 acggtgcttt ccgccggacc gcgcgcgctc ggcttcatcg aaggtgcagc cgaggcgacg
     1201 gcggcgctgc tcaagctcgt ctccggcgtc tggtacgacc gcgtacggcg tgccaaggga
     1261 ttcgtcgtcg tcggctacgg cctggccgcc gtcgctcgac cgctgatcgc cgtggtgacc
     1321 gcctggcccg gcctcctgtt gctgcgcgtg ctcgaccggc tcggcaaggg cctgcgctcg
     1381 tcgccgcgcg atgccctgct ggcccattcg gttccggccg aacggcgcgg actcgcgttc
     1441 ggccttcatc gagctttcga caacgccggc gccgtgatcg ggccgctctt ggcggcggcc
     1501 ctgctggcac tgcacgtgcc gatccgcgac attctgctgt gggcagcatt gccgggggcg
     1561 ctgtgcgttg cgctcgcgtt gtccatccga gaaccgctca accttgtcgt tccacatgcc
     1621 cgccgggccg agtgggcctg gcgcacgctg ccgtttgctt ttcgtcgcat gctggtcgcg
     1681 ctggccctgt tcacgctcgg gcaggcgtcg aacgccttca tcctgctgcg tgccactgaa
     1741 cttggcatgt ctgcaacgca ggtggcactg ctgtgggcgg tggtgtcggc cacgtcgacg
     1801 ctgctggcgg tgccgctgtc ggcgtggtca gaccgcatcg ggcgcgtgcc gctgctggtc
     1861 ggcggctgga ccctacatgc gctgctgttc gttgcgcttg cgctggtgtc cgcgcctgcg
     1921 ctgctgtggg gcatcgcggt cctgctcggc atctacatgg cgtcgaccga aggtgcggag
     1981 cgggcgctga ttgccgatct cgtcgggccc gactctctgg gcacggccta tggctggtac
     2041 tacctgacga aagggctgct gctgttgccg gcttcggcgg ctttcggctg gatctggttt
     2101 gcccgggacg ccgcgtcggc cttcctcacc gccgcggccc tggtggcgac agccacgatc
     2161 gtgctggcgc tgtgggtgct gcccgcggtc aacgcgcatc gtcgcgccgt ccgtcgcggc
     2221 tgaagcatcg ggctatcgcg tgcctgacct agagaacgcg caccagcgtg taggccagca
     2281 tgcccagcac aatcgcagcg acagcacggc gcgtgaacac gatgacggtt accgccacga
     2341 gggtcgcaat cagcttcggg ttggcgaggc cga
//
LOCUS       DATIRT010000251         7076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1904779, whole genome shotgun sequence.
ACCESSION   DATIRT010000251 DATIRT010000000
VERSION     DATIRT010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7076
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1904779"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VLN25_05780"
     CDS             complement(<1..513)
                     /locus_tag="VLN25_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HSQ10108.1"
                     /translation="MNAPDSISRRSNLDAALLARTSPLPISTYFDPAWLERELLLLFD
                     RGPNYVGSRMMVPEVGDTMTLPWTDHGKVLVHVPSAVNLLSNTCSHRQGLLLEGRGNH
                     QNIVCPLHRWTYDLDGRLLGAPDFEETPDCSLPRTPLTEWNGLLFAGPRDAAADLAAF
                     PLATDYDYSKY"
     gene            complement(594..3218)
                     /locus_tag="VLN25_05785"
     CDS             complement(594..3218)
                     /locus_tag="VLN25_05785"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HSQ10109.1"
                     /translation="MQSSLAPAPKFVTPPPSTWHAIAADAAVEQLASGHQGLSAAAAA
                     DRLQRDGPNLLPEVAPVSMLAMFARQFADFMIGVLIVAAVVAAVIGEPVESVAILAIV
                     VLNAILGFTQEWRAEQAMAALRALSAPRARVIRDGARMEIPAADLVTGDVVWLEAGTK
                     VPADLRLVESAALRIEEATLTGESVPADKDAGAVLGADAVLGDRRNMAYSGTMVTAGR
                     GLGLVIATGAATELGHIAQLVGSTGEQRTPLQDRLARFGRRLALIVLAICAVIFVAGI
                     FRGEPATLMLLTAISLAVAAIPEALPAVVSIALAFGAHRMSRERALVRRLPSVESLGS
                     VTVICSDKTGTLTENRLRVERLELTSESARERALKVLALNSDASRSPEGLWVGDPTEI
                     ALVDAAAAAGVDVDAMRAASPRIAELPFDSESKRMSTLHADQGAALLTVKGAPESVLA
                     CCTNIDRDEWLQRSERLAAAGQRVLALAARSARLDESIDQLDSRLELLALVGLIDPAR
                     PEAATAVAECMSAGVVPVMITGDHPATALAIARSLGMVGSEARAMTGLELATIDASAL
                     PDRVHHVQVYARVDPAQKIRIVEALQARGECVAMTGDGVNDAPALRKADIGVAMGRGG
                     TDVAREASDLVLLDDNFATIVAAVREGRRIYDNIRKFIRFVMAGNAGEIWTIFLAPFL
                     GLPIPLSPIQILWVNFVTDGLPGLALAGEPAEPHVMRRPPRPRNESVFAHGVWQHILW
                     AGLLIGGISLYACYWAIENGEHAQTMTFTVLTFAQLAHVMAIRSERESLFTIGVASNR
                     LLLLAVAASVALHLLLIYVPWLQPVFGTETLSAHALVLACALPLVVFGGVEVEKWAVR
                     QGWLYRDSAQLAARVSGM"
     gene            3405..3971
                     /locus_tag="VLN25_05790"
     CDS             3405..3971
                     /locus_tag="VLN25_05790"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HSQ10110.1"
                     /translation="MTEPNSDAPTQPAAASNRKRRWTVLVLGVATLVTVGALTAHRHA
                     EAGAMGFGGHGHGHGPGGWHGGMGDMDPAVMAKRIDARVAYMLAEIDATPEQRERIAK
                     ILKGLANDMQQNRQRHVQARQQSLELLAAPTIDRAQLEKLRVEQMQLGDVTSRRMLQA
                     MMDSAEVLSPEQRAKLIERWQRRMPPRK"
     gene            4079..4771
                     /locus_tag="VLN25_05795"
     CDS             4079..4771
                     /locus_tag="VLN25_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSQ10111.1"
                     /translation="MQRLLMIDDDVRLAAMVRDYLGPSGFDVDVAADAASGLAALRSR
                     PAELLILDLMLPDSDGLDVCRRLRAEGFGLPILMLTAKGDPIDRVIGLELGADDYLPK
                     PFEPRELLARVKALLRRATGTPATATLRFGRLELDRAGRVVRVDGQVRSLTSHQFELL
                     VALAERAGRVLTREQLLDLVRGEALEAFDRSVDVHIARIRAAVEDDPRNPKRIITVRG
                     AGYVFARSQDVA"
     gene            4830..5633
                     /locus_tag="VLN25_05800"
     CDS             4830..5633
                     /locus_tag="VLN25_05800"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HSQ10112.1"
                     /translation="MTAARLGRHRLYWRIWLAILATVLLFALLAGAAWRLLGERPQPP
                     NVTGFAELASELLPAANAPPAEQQAAVNHWRGRLGGDYALYSRDGVLVARTSEDLVMR
                     TRHDEEPDHQLMTSRGPAIVLSLPDGRALLVLRPGRPPPPGPILTFLLIALGIGVGAN
                     PVVRRLTQRLERLQEGVTEWGAGNLGARVAIEGRDEVAGLAASFNDAAVRIEQLVNVH
                     KSLLANASHELRSPLARIRLGVELMAVDPSPARQAELARDIGELDQLID"
     gene            5730..6092
                     /locus_tag="VLN25_05805"
     CDS             5730..6092
                     /locus_tag="VLN25_05805"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSQ10113.1"
                     /translation="MPLQADAVTVDGNARLLQRLVRNLLENAARYGGGRVEAALRASD
                     DAITFDVLDRGSGISATERERIFEPFYRAAGGSESSGGVGLGLSLVRQIARQHGGDVQ
                     CLPRDGGGSIFRAAFSRR"
     gene            complement(6112..6279)
                     /locus_tag="VLN25_05810"
     CDS             complement(6112..6279)
                     /locus_tag="VLN25_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10114.1"
                     /translation="MTLFEWLAAVCGELGWNVMRSHDMLDETRWSDLYEQGLSPQDAA
                     ARARAEAWLGD"
     gene            complement(6290..>7076)
                     /locus_tag="VLN25_05815"
     CDS             complement(6290..>7076)
                     /locus_tag="VLN25_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HSQ10115.1"
                     /translation="GGIGMVILVVVGMLFGIDPRVILGLAGTVETVQNMGASKQQVQG
                     KAGPPSDEMGRFVAGVLADTEKTWGTLFQQLGRQYRNPALVLFTGATPTACGTGQTAM
                     GPFYCPLDQKVYIDLQFYQALRDKFRAPGDFAQAYVISHEVGHHVQNQLGIMQKMDEY
                     RRRMNETQYNALSVRLELQADCFAGVWAHHAGNRNLLEAGDVEEALRAAAAIGDDTIQ
                     HRTQGRIVPDSFTHGSSEQRMRWFMTGMKSGQIAACNTFDASI"
BASE COUNT         1184 a         2347 c         2372 g         1173 t
ORIGIN      
        1 gtacttcgag tagtcgtagt cggtcgcgag cggaaacgca gcaagatccg ccgccgcatc
       61 gcgcggcccg gcaaacagca gcccgttcca ttccgtcagc ggtgttcgtg gcagcgagca
      121 atcgggcgtt tcctcgaagt cgggcgcacc cagcaggcgg ccgtcgagat cgtaggtcca
      181 gcggtgcagc ggacagacga tgttctgatg attgccgcgg ccctcgagta gcagtccctg
      241 ccggtgactg caggtgttcg acagcaggtt gacggcgctc ggcacgtgca ccagcacctt
      301 gccgtggtcg gtccagggca gcgtcatcgt gtcgccgact tccggcacca tcatgcggct
      361 gccgacgtaa ttcggaccgc gatcgaacag gagcaggagt tcgcgctcga gccatgcggg
      421 atcgaagtac gtggaaatcg gcagcggcga tgtgcgcgcc aggagtgcag cgtcgaggtt
      481 ggaccggcgc gagatcgagt ccggggcgtt catggcgtga cgtcggaggt aggctcaggc
      541 ggactgctga aatcgataat acccgcggcg tgctgtcatc agcgtgacat ccgctacatt
      601 cccgaaaccc gtgcggccag ctgtgcggaa tctcgataca gccatccctg tcgcacggcc
      661 catttctcca cttcgacgcc gccgaacacg accaggggga gtgcacaggc gagcacgagc
      721 gcgtgcgccg acaaggtctc tgtcccgaaa accggctgca gccacggcac gtagatcagc
      781 aggaggtgca gtgcgacgct cgcagcgacc gccagcagca gcaggcgatt gctggcgacg
      841 ccgatcgtga aaaggctctc gcgttccgag cggatcgcca tcacgtgggc cagttgggca
      901 aacgtcagca cggtaaaggt catcgtctgc gcatgctcgc cgttttcgat ggcccagtag
      961 caggcataca ggctgattcc gccaatcagc agcccggccc agaggatgtg ctgccacacg
     1021 ccgtgcgcga agaccgactc gttgcgcggt cggggcggac gtcgcatgac atggggctcc
     1081 gccggttcgc cggccagcgc gaggcccggt aacccgtcgg tcacgaagtt gacccacagg
     1141 atctggatcg gcgacagcgg aattgggaga ccaagaaacg gcgccaggaa gatcgtccag
     1201 atctcgcctg cgttgcccgc catcacgaag cggatgaact tgcggatgtt gtcgtagatg
     1261 cggcgaccct cgcgcacggc cgccacgatc gtcgcgaaat tgtcgtccag cagcacgagg
     1321 tcggacgcct cgcgcgccac atccgtcccg ccgcggccca tcgccacgcc gatgtctgcc
     1381 ttgcgcagtg ccggcgcatc gttgacgccg tctccagtca tcgcgacaca ctcgccgcgc
     1441 gcctgcagag cctcgacgat gcgtatcttc tgtgccggat cgacccgcgc gtagacctgc
     1501 acgtgatgga cacggtcggg cagagcactg gcatcgatgg tggccagctc caagccggtc
     1561 atcgcgcgag cctcggaacc gaccatgccg agcgagcgcg cgatcgccag cgcggtggca
     1621 ggatgatccc cggtgatcat taccgggacg acgccggcgc tcatgcactc ggccacggcg
     1681 gtcgcggcct cgggccgcgc gggatcgatc agtccgacca gcgcaagcag ttcaaggcgc
     1741 gagtccagct ggtctattga ctcgtcgagc cgcgcggagc gtgcggccag cgccaatacg
     1801 cgctgacctg cggcggcgag acgttcggag cgctggagcc actcgtcgcg gtcgatgttg
     1861 gtgcagcacg ccagtacgga ctccggcgcg cccttgacgg tcagcagtgc ggcgccctgg
     1921 tctgcatgca gtgtcgacat gcgcttgctc tcggagtcga acggcagctc ggctatgcgt
     1981 ggcgaagcgg cgcgcatagc atcgacgtcc acgcccgcgg ccgccgcggc atcgacgagg
     2041 gcgatctcgg tcgggtcgcc gacccagagt ccctcgggcg agcgggacgc atcgctgttc
     2101 agggccagca ccttcagcgc gcgttcacgt gcgctttcgc tggtgagttc gagccgttcc
     2161 acgcgcaggc ggttctccgt cagcgttccg gtcttgtcgc tgcagatcac cgtgaccgag
     2221 ccgagcgact cgacgctcgg tagccggcgt accagcgccc gctcgcgcga catgcgatgc
     2281 gcgccgaacg cgagcgcgat cgacacgacc gccggcaggg cttcgggaat cgcggccacc
     2341 gcaaggctga tcgcggtcag gagcatcaag gtcgcgggct cgccgcggaa gataccggcg
     2401 acaaagatga cggcgcagat ggcgaggacg atcagcgcca gtcggcgacc gaaacgcgcc
     2461 aggcggtcct gcagcggagt gcgctgctcg cccgttgagc ccaccagctg cgcgatgtgg
     2521 ccgagttcgg ttgccgctcc ggttgcgatc acgaggccca gtccacgacc ggccgtcacc
     2581 atcgttcccg agtacgccat gttgcggcgg tcgccgagca cggcatccgc cccgagtaca
     2641 gcgccggcgt ccttgtcggc cggtacggac tcgcccgtca aagtggcttc ttcgatgcgc
     2701 agagcggcgc tttcgaccag gcgcaggtcg gccggaacct tggttccggc ctcaagccag
     2761 acgacgtcgc ccgtgacgag atctgcggca ggaatctcca tgcgggcacc gtcgcggatc
     2821 actcgtgcgc gcggcgccga cagtgcgcgc agtgccgcca tggcctgctc tgcgcgccat
     2881 tcctgggtga atccgagaat ggcgttcagc acgacgatgg cgagaatggc gacggactcg
     2941 accggctcgc cgatgaccgc ggcgaccacc gccgccacga tcagcacgcc gatcatgaag
     3001 tcggcaaact gtcgcgcgaa catcgcgagc atgctgaccg gcgcgacttc cggcagcaga
     3061 ttcggcccgt cgcgctgcag ccggtcagcg gccgctgccg cggatagacc ctgatggccg
     3121 gatgcaagct gctccacagc ggcgtccgca gcgatggcgt gccacgtgct gggaggaggc
     3181 gtcacgaatt ttggcgcggg agcgagactg gattgcacgg cgttgacgct gcagtcaacc
     3241 gctagggtag gggttgccgg gtctaagggc gctgacctgg gtccaatgca gccccgattt
     3301 ccacgcccgg tttacacatc tttacaacct ggccgaacac cggcaacgca ggctccctac
     3361 agtgcgatca ccaccccgac gatgatgaac acagggagca tgcaatgaca gaaccgaact
     3421 ccgacgcgcc cacgcaaccc gcggctgcgt ccaaccgcaa gcggcgctgg acagttctgg
     3481 tcctcggcgt ggccaccctg gtcactgtcg gtgcgctgac cgcacatcgg catgcagagg
     3541 ctggcgccat gggattcggc ggccacggtc atggacatgg gcccggcggg tggcatggcg
     3601 gcatgggtga catggacccc gcggtgatgg ccaagcgcat cgatgcacga gtggcctaca
     3661 tgctggccga aatcgatgcg acgcccgagc agcgcgagcg catcgcaaag atcctgaagg
     3721 gcctggcgaa cgacatgcag cagaatcgcc aacgccacgt gcaggcgcgt caacagtcgc
     3781 tcgaactgct cgcagcgccg accatcgatc gcgcgcagct cgagaaactg cgcgtcgagc
     3841 agatgcaact gggcgacgtg acatcgcgcc ggatgctgca ggcgatgatg gacagcgccg
     3901 aagtgctgag cccggagcag cgcgccaaac tgatcgaacg ctggcagcgc cgcatgccgc
     3961 ctcgcaagta acctttaccg ccgcggcccc ggacccctcc ccgaatcccg gcggcccctc
     4021 cacctcaccg gcattcaccg gtggccgcgg ctcctccctc ctgcttaaga tccaccgtat
     4081 gcagcggctg ctgatgatcg acgacgacgt gcgccttgcg gccatggtgc gcgactacct
     4141 cgggccgtcg ggcttcgacg tcgacgtggc cgccgatgcg gcgagcggac tcgccgcgct
     4201 tcggtcacgg ccggccgaac tgctgatcct cgatctgatg ctgccggaca gcgatggact
     4261 cgacgtctgt cgccgcctgc gcgctgaagg cttcggattg ccgatcctga tgctgacggc
     4321 caaaggtgac ccgatcgatc gtgtgatcgg actcgaactc ggcgccgatg attacctgcc
     4381 gaaaccgttt gagccgcgcg agttgctcgc gcgcgtcaag gcgctgctgc ggcgtgccac
     4441 cggaacgccc gctacggcga cgttgcggtt cggtcggctc gaactggatc gcgcgggccg
     4501 tgtggttcgc gtcgatggcc aggtccgctc gctgacgtcg catcagttcg agctgctggt
     4561 cgctctggcc gagcgtgcag ggcgcgtact gacgcgcgag caattgttgg acctggtgcg
     4621 tggcgaggcg ctcgaggcct ttgaccgctc ggtcgacgtg cacattgccc gcatccgcgc
     4681 cgccgtggag gacgatccgc gcaatccgaa gcgcatcatc accgtgcgtg gtgcaggcta
     4741 cgtctttgcg cggtcgcagg atgtggcgtg atcaggcccg gcgaactgga cggcgattgc
     4801 gttctattgc gtgacgcgga ggcacgccag tgaccgcagc gcgtctcggc cgacatcgcc
     4861 tgtactggcg catctggctg gcgatccttg cgaccgtgtt gctgttcgca ttgctggctg
     4921 gcgcggcctg gcgactgctc ggcgagcggc cgcagcctcc caacgtcact ggatttgccg
     4981 agctggcaag cgagctgttg cccgcggcaa atgcgccgcc ggccgaacag caggcggcag
     5041 ttaaccactg gcgcggtcgc ctgggcggcg actacgcgct gtattcgcgc gatggcgtgc
     5101 tggtggcccg caccagcgag gatctggtga tgcggacccg gcatgacgag gaacccgatc
     5161 atcaactgat gacttcgcgg ggtccggcga ttgtgctgtc gctgcctgac ggtcgcgcgc
     5221 tgcttgttct tcgcccgggt cggccgccgc cacccgggcc gatactgacc tttttactga
     5281 tcgcactcgg catcggtgtc ggggccaatc ccgtcgtgcg tcgattgacg caacggctcg
     5341 agcgactgca ggaaggagtg accgaatggg gcgcgggcaa tctgggcgcc cgggttgcca
     5401 tcgagggccg cgacgaagtc gccggactcg cggccagctt caatgacgcc gccgtgcgca
     5461 tcgagcagct cgtcaacgtg cacaagtcgt tgctggcaaa cgcctcgcat gagctgcgct
     5521 cgccgctcgc tcgcatccgc ctcggtgtgg aactgatggc ggtcgatccg tccccggcgc
     5581 ggcaggcgga gctggcgcgc gacatcggcg aactcgacca gctgatcgat taaatcctgc
     5641 tggcgagtcg gctcgaaggc ggcgtcgcac caaagcacga gccggtcgat ttcaccgccg
     5701 tcgttgcaga agagtgcgca cgcttcaacg tgccgctgca ggccgacgcc gtgaccgtcg
     5761 acggcaatgc ccggctgctg cagcgcctgg tgcgcaattt gctcgaaaat gccgcgcgct
     5821 acggcggcgg tcgcgttgaa gcggcgctgc gcgcatcgga cgatgccatc acattcgacg
     5881 ttctcgaccg gggttcgggc atcagcgcca ccgagcggga acggatcttc gagccgttct
     5941 accgggccgc cggtggcagc gagtccagtg gcggcgtggg tctcggcctg tcgctggtgc
     6001 ggcaaattgc tcgccagcac ggtggcgatg tgcagtgcct gccgcgcgac ggcggcggca
     6061 gcatcttccg tgctgctttt tctcgccgct agaggcgcgc cagactaggc actagtcacc
     6121 cagccaagcc tcggcgcgtg cgcgtgcagc ggcatcctgc ggactgagtc cctgctcgta
     6181 caggtcggac cagcgggttt cgtcgagcat gtcgtgcgat cgcatgacgt tccagccgag
     6241 ttccccgcat accgccgcga gccattcgaa cagcgtcatg gcgcccggat cagatgctgg
     6301 cgtcgaaggt attgcaggct gcgatctggc cgcttttcat cccggtcatg aaccaacgca
     6361 tgcgttgctc ggagctgccg tgcgtgaagc tgtcgggcac gatgcgacct tgggtgcgat
     6421 gctggatcgt gtcatcgccg atcgcggctg cagcgcgcag cgcttcttcc acatcgccag
     6481 cctcgagcag gttgcggttg cccgcatggt gcgcccagac gccggcgaag cagtcggcct
     6541 ggagttcgag ccgcacggac aaggcgttgt actgcgtctc gttcatgcgg cgtcgatact
     6601 catccatctt ctgcatgatg cccagctggt tctgcacgtg atgcccgacc tcgtgcgaga
     6661 tcacatacgc ctgtgcaaaa tcccccggcg cgcgaaactt gtcccgcagc gcctggtaga
     6721 actgcaggtc gatgtagacc ttctggtcga gcgggcagta aaacggcccc atcgccgttt
     6781 ggccagtgcc gcacgccgtg ggggtcgcgc cggtgaacag caccagtgcc ggattacggt
     6841 actggcggcc cagctgctgg aacagcgtgc cccaggtttt ctccgtatcg gccaacacgc
     6901 cggccacgaa gcgacccatc tcgtcgctgg gtgggccggc cttgccctga acctgctgct
     6961 tagaggcgcc catgttctgc accgtctcga ccgtacccgc gagtccgagg atcacgcgtg
     7021 gatcgattcc gaagagcatc ccgaccacga ccaggatcac catcccgatg ccgccg
//
LOCUS       DATIRT010000252         2159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_234204, whole genome shotgun sequence.
ACCESSION   DATIRT010000252 DATIRT010000000
VERSION     DATIRT010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234204"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="VLN25_05820"
     CDS             complement(<1..992)
                     /locus_tag="VLN25_05820"
                     /inference="COORDINATES: protein motif:HMM:NF023516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HSQ10116.1"
                     /translation="MDTTLKMPRRGAVLAAALALPGLMPGGAQAQVTAPNATLVQFKY
                     LYYKDYQASGDRMTVKSPALYLLAPITDDWVFEIGAVYDSISGASPYYHTTLSGASGL
                     GIKDARTAVDVTVTKYFANRTSVAIRAAYSDEDDYRSTAGAVTIRHVTPDQNTTFTVG
                     GGYSGDEVFPTVGTQPPPTRQKRTYDAIVGLSQVFSPNDTAQFNLYYAHQSGYLTDPY
                     KAYWGIDNRPATRDQFAGLVRWNHFFDSANATLRMSYRYYQDDWEVVANTVTFDWAQT
                     LPDGWLLTPGLRYTTQSAAIFYYDPIYDPVLGAPYPPGYLNNPGGFYTSDQRLSA"
     gene            complement(998..1219)
                     /locus_tag="VLN25_05825"
     CDS             complement(998..1219)
                     /locus_tag="VLN25_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HSQ10117.1"
                     /translation="MTKRLLITIAAAVLVTGCESMPKVQPWQKGNLAKPEMAFDSDAL
                     EARFADHTYFSKEGASGGASVGGGGCGCN"
     gene            complement(1227..1730)
                     /locus_tag="VLN25_05830"
     CDS             complement(1227..1730)
                     /locus_tag="VLN25_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HSQ10118.1"
                     /translation="MRSKKLFVAVCAALFVSAVSAKDVGKAAPAFELPAAGAQKVRLA
                     DLKGRVVLVDFWASWCAPCKQSFPWMNEMQAKYGPKGLTIIGINVDKKREDADKFLAG
                     TPAKFTVAFDSAGKVAESYQPQGMPTSFLIGADGVVRAMHVGFREGDREKLEAELAAA
                     VSAAARK"
     gene            complement(1800..>2159)
                     /locus_tag="VLN25_05835"
     CDS             complement(1800..>2159)
                     /locus_tag="VLN25_05835"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HSQ10119.1"
                     /translation="DPTGISYREVFEIFFAIHDPTTLSRQGNDVGTQYRSAIYLQSAE
                     QRSVAEDVIREAQAHYRDRIVTELVEAGPYWRAETYHQEYFRHNPEQGYCAVVVAPKV
                     AKFRKTFAAKRRNSAGD"
BASE COUNT          383 a          692 c          679 g          405 t
ORIGIN      
        1 gccgagaggc gctggtccga ggtgtagaag ccgcccggat tgttcaggta gcccggcgga
       61 tacggtgccc cgagcaccgg gtcgtagatc gggtcgtaat agaaaattgc tgcgctctgc
      121 gtcgtgtagc gcaacccggg agtcagcagc cagccatcgg gaagcgtctg cgcccagtcg
      181 aacgtgacgg tgttggccac cacctcccag tcgtcctggt agtagcgata ggacatgcgc
      241 aacgtcgcat tggcggaatc gaagaagtgg ttccaccgca ccagacccgc gaactgatcg
      301 cgcgtggccg ggcggttgtc gatgccccag tacgccttgt aggggtcagt cagataaccg
      361 ctctggtgcg cgtagtacaa attgaactgg gcggtgtcgt tcggcgagaa gacctgcgac
      421 aggccaacga tggcgtcata ggtccgcttc tgccgcgtgg gcggtggttg ggttccgacg
      481 gtgggaaaga cttcgtcgcc agaatacccg ccgcccacag taaacgtcgt gttctgatca
      541 ggcgtcacat gccggatcgt cacggctcct gcggtcgagc ggtagtcgtc ttcgtccgag
      601 taggccgcgc ggatcgccac tgaagttcga ttcgcgaaat acttggtgac cgtgacatcc
      661 accgctgttc gcgcatcctt gatacccagg cccgacgccc ccgacagcgt cgtgtggtaa
      721 tacggcgacg cgcccgagat gctgtcgtag accgcgccga tctcgaacac ccagtcgtcg
      781 gtgatcggcg ccagcaggta aagcgccggc gacttcaccg tcatgcggtc gccggacgct
      841 tgatagtcct tgtagtagag gtacttgaac tgcaccagcg tcgcgttggg tgctgtgacc
      901 tgtgcctgcg ctcctcccgg catcagtccg ggcagcgcaa gcgccgcggc cagaaccgcg
      961 ccgcgtcgtg gcatcttgag cgtggtatcc attgtgtcta gttgcagccg catccgccgc
     1021 ctccgacact ggcaccgcct gacgcgcctt ccttgctgaa gtaggtgtgg tcggcgaagc
     1081 gcgcctcgag cgcgtctgaa tcgaatgcca tctcgggctt ggccaggttg cccttctgcc
     1141 agggctgaac cttcggcatc gactcgcagc cggtcaccag cacggcggca gcgatcgtga
     1201 tcaacaagcg cttcgtcatc gtgtcctcac ttgcgtgcgg cggccgacac cgcggcggcc
     1261 agttcggcct cgagcttctc acggtcgcct tcgcggaagc cgacgtgcat cgcccgcacc
     1321 acgccatcgg cgccgatcag gaacgacgtc ggcatgccct ggggctggta cgactcggcg
     1381 accttgccag cggagtcaaa ggccaccgtg aacttcgccg gcgtgccggc gaggaacttg
     1441 tcggcgtcct cgcgcttctt gtcgacgttg atgccgatga tcgtcaatcc cttgggcccg
     1501 tacttggcct gcatctcgtt catccaggga aacgactgct tgcaaggcgc acaccaggac
     1561 gcccagaagt cgaccagcac gacgcgaccc ttgaggtccg ccagtcggac cttctgcgca
     1621 ccggccgccg gcagttcaaa ggcgggggca gccttgccga catcctttgc cgaaacagca
     1681 ctgacgaaca atgcggcaca gaccgcaaca aacagtttct tggaccgcat gcacatcctc
     1741 cccgatgacg accgtgcgat ggtagcggca ccatcgcact gcgacatcgc ggattgcggc
     1801 taatcccccg ccgaattccg acgttttgcc gcgaaggtct tcctgaactt cgccaccttg
     1861 ggcgcaacga cgacggcgca gtagccctgc tccggattgt ggcggaagta ctcctggtga
     1921 taggtctcgg cacgccagta gggcccggcc tcgacgagtt cggtcacgat ccggtctcga
     1981 tagtgggcct gcgcctcccg aatgacgtcc tcggccacgc tacgctgttc ggctgactga
     2041 aggtagatgg ccgaccggta ctgcgtgccg acgtcgttgc cctggcgact gagcgtggtc
     2101 gggtcatgga tcgcaaagaa gatctcgaac acctcgcggt aggagatacc cgtaggatc
//
LOCUS       DATIRT010000253         5574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1217861, whole genome shotgun sequence.
ACCESSION   DATIRT010000253 DATIRT010000000
VERSION     DATIRT010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5574
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217861"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(433..508)
                     /locus_tag="VLN25_05840"
     tRNA            complement(433..508)
                     /locus_tag="VLN25_05840"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(473..475),aa:Thr,seq:tgt)
     gene            complement(530..1051)
                     /locus_tag="VLN25_05845"
     CDS             complement(530..1051)
                     /locus_tag="VLN25_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HSQ10120.1"
                     /translation="MSELSTKPYLIRAIHQWCTDSGFTPYIAVLADEGVRVPREFVKN
                     GEIVLNISPLATNRLQLGNDAIEFQARFGGVAREVFVPIEKVLAVYARENGQGMAFEV
                     PRDAAPAGVEQPAPGSVNAPRPPMALVPVQGAPSTQESLTESSNGDEADKNTPDDTPP
                     APRGERPKLTRVK"
     gene            complement(1070..1672)
                     /locus_tag="VLN25_05850"
     CDS             complement(1070..1672)
                     /locus_tag="VLN25_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HSQ10121.1"
                     /translation="MMVLYSGTTCPFSQRCRFVLFEKGMDFEIRDVDLFNKPEDINVM
                     NPYGQVPILVERDLVLYESNIINEYIDERFPHPQLMPADPVMRARTRLFLFNFERELF
                     VHVQALENSNNQKLIEKGRSTIRDRLTQLAPIMLKNKYMLGDDFSMLDVALAPLLWRL
                     DHYGIDLPKTAAPLMKYAERIFSRPAYIEALTPSEKVMRR"
     gene            complement(1847..2704)
                     /locus_tag="VLN25_05855"
     CDS             complement(1847..2704)
                     /locus_tag="VLN25_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HSQ10122.1"
                     /translation="MRDIITRACIALGLLVSVGSASAAGASIPIETWPSERSRDFGSL
                     QNGARLFANYCMGCHSANLVRWNRLQQIGLDDKQIKEFLIFGSQKVGDPMKIPMAPSD
                     AKVWFGKVPPDLSVITRARTTFDFKGTDYIYTLLRGYYRDNTTATGWNNIAYPNIGMP
                     HIFWDRQGPREATLVEVHRHTDAKTGKSGFVRTTTTFDPAGNATRTEAEAQPPVSEGF
                     IYSFRPLDAPQARQYDSDVADLVAFLAFITDPSATSRVLIGVWVMLFLALFTVLAWRM
                     NAVFWKDIK"
     gene            complement(2722..4104)
                     /locus_tag="VLN25_05860"
     CDS             complement(2722..4104)
                     /locus_tag="VLN25_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HSQ10123.1"
                     /translation="MAEYKSPYTGLAGWFDQRMPTVMAEYKKHLSEYYAPKNFNVWYI
                     FGGLAITVLAIQIITGIFLVMHYKPDANLAFASVEYIMRDVPGGWFIRYMHSTGASFF
                     FIVVYLHMFRGLMYGSYRTPRELVWIFGCLIFLCLMGEAFFGYLLPWGQMSYWGAQVI
                     INLFGAIPLIGPDLSVWVRGDYVIGDATLNRFFAFHVIAIPLVLIGLVVAHILARHDV
                     GSNNPDGVEIKKNLDASGKPIDGIPFHPYYTVHDIFWLAIFLILFFAVVFFAPEMGGY
                     FLEWNNFIPADPYQGPAHIAPVWYFTPYYSILRATTEQFMAVIAVGTIVLSALILMTL
                     RNPVVRGAIAVGTLVLLLGYFTLDAKFWGVVLMGASVMIFLGLPWLDQSPAKSMRYRP
                     GWHFWLLIVWVVAFIVLGYYGTQPPSTAGQYASMIGTVIYFGFFALMPWWSRMGTFKP
                     VPDRVTFHPH"
     gene            complement(4120..4740)
                     /gene="petA"
                     /locus_tag="VLN25_05865"
     CDS             complement(4120..4740)
                     /gene="petA"
                     /locus_tag="VLN25_05865"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HSQ10124.1"
                     /translation="MSTSTSVPAGEMPAGVDKERRNAIAVAATVGGAGAVALAVPFVS
                     SFAPSERAKAAGAPVEFDLAVLAPGEMKTVEWRGVPVWVLRRTPEMLQSIKKADGEVA
                     DPKSLRTAFPTPEYARNEYRSRKPEFLITVGICTHLGCNPVGPFGPGQNAQLGADQGF
                     LCPCHGSTFDLAGRVFKNKPAPDNLVVPPHKYMSETVIVIGDDTNA"
     gene            4966..5385
                     /gene="mscL"
                     /locus_tag="VLN25_05870"
     CDS             4966..5385
                     /gene="mscL"
                     /locus_tag="VLN25_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HSQ10125.1"
                     /translation="MGMMKEFREFAVKGNVIDLAVGIIIGAAFGKIVDSLVRDVVMPL
                     INAVFGGAVDFSNKYWVLSGNVPAGASLDAAQKSGVVFAWGNFATVLLNFVILAFVIF
                     WMVKAVNRVKANLDAAAAPTPEEVALLREIRDELKRR"
BASE COUNT         1090 a         1715 c         1761 g         1008 t
ORIGIN      
        1 tggcggaggg tttccgtccc cgaatccatc cgtgaagaac gtgcctccgt tccttttgat
       61 ttcgaattga tcgaggtaca cgactgctgc gttcgcaccg acagaccaga agcaggttgc
      121 aagtacaacc accgctgggg caatcctact gaacgtacgc attcggtccc cctcttgtag
      181 gaaatcccta cccgaactgg ggtaaagcaa aaagcgtgcg ttctcaagaa aacagacagt
      241 tacgacgcaa gtgggtgcgg tgtgtaagaa aagccgtcgt cgggaatctg acgaggggag
      301 gtctgcaagc ccgcagagac gggccgcact cacagtccaa tcgaccccag gctcgcactc
      361 cggcatctct tgcgcaatca cgggcgagat gtcggagtga ttttgttgcg tcggcacgag
      421 aattggaagc gctggtgccg gcagagggaa tcgaaccccc gaccttcggt gtacaaaacc
      481 gctgctctac cagctgagct atgccggcat gatgtatggc aagttgtggc tacttgacgc
      541 gtgtgagctt cggccgctcg ccccgcggag ctggcggcgt gtcgtccggc gtgttcttgt
      601 ccgcctcatc gccgttggac gattcagtga gggactcctg ggtcgatggc gcgccctgga
      661 ccggaacgag cgccatcggc ggccgcgggg cgttcacgct ccccggcgcc ggctgctcga
      721 cgccggccgg cgcggcatcg cgaggcacct cgaacgccat gccctgtccg ttttcgcgcg
      781 cgtacaccgc gagcaccttt tcgatcggga cgaacacttc gcgcgccact ccgccgaagc
      841 gggcctggaa ttcgatcgca tcgttgccca gctgcaatcg attcgtcgcc agcggcgaga
      901 tgttgagcac gatctccccg ttcttgacga actcgcgcgg cacgcgcact ccctcatcgg
      961 ccaggaccgc aatgtatggc gtgaagccgg aatccgtgca ccactggtga atggcgcgga
     1021 tcaggtacgg cttggtcgag agttcagaca tcggaacgtc agtgcgcgct caacgccgca
     1081 tcactttctc ggagggcgtc agggcctcaa tgtacgccgg ccgggagaag atgcgctcag
     1141 cgtacttcat cagcggtgct gctgtcttgg gcaggtcgat gccgtagtga tccaggcgcc
     1201 acagcagggg tgcgagcgcg acatcgagca tcgagaagtc gtcgcccagc atgtacttgt
     1261 tcttcagcat gatcggcgcc agctgggtca gccgatcgcg gatggtcgag cgcccctttt
     1321 cgatcagttt ctggttgttg ctgttctcca gcgcctggac atgcacgaac agctcccgct
     1381 cgaagttgaa caggaacagg cgcgtgcgcg cccgcatcac cgggtccgcc ggcatcagct
     1441 gcgggtgcgg aaagcgctcg tcgatgtact cgttgatgat gttcgactcg tacagcacga
     1501 ggtcgcgttc gaccaggatc ggtacctgcc cgtagggatt catcacgttg atgtcctcgg
     1561 gcttgttgaa cagatccaca tcgcggatct cgaagtccat gcccttttcg aacaacacga
     1621 aacggcagcg ctgcgagaac gggcaggtgg ttccagagta gagcaccatc atcttttgct
     1681 ttccttcgtg cgtcggcgag ccgggtccgg ctcgtgcagc gccgacgccc gaagggcatc
     1741 ggcagcttgt tgaatgcagg agcgccggtc cgtcccaagc tcctgcatct gataaggccg
     1801 ggcgaggtgc acgtgcggcg cgaagcgacg cgtacaccga gcccgactac ttaatgtcct
     1861 tccagaacac cgcgttcatg cgccacgcca acacggtgaa aagcgccagg aacagcatga
     1921 cccagacgcc gatgagcaca cggctcgtcg ccgacgggtc ggtgatgaag gccaggaacg
     1981 ccaccaggtc cgccacgtcc gagtcgtact gccgcgcctg cggcgcatcc agcggccgga
     2041 agctgtagat gaaaccttcg ctcaccggcg gctgcgcctc ggcctcggtc cgggtcgcat
     2101 tgccggcggg atcaaacgtc gtcgtcgtgc gtacgaagcc cgacttgccg gtctttgcgt
     2161 cggtgtgtcg gtgcacttcg acgagcgtgg cttcgcgcgg cccctgccgg tcccagaaga
     2221 tgtgcggcat gccgatgttc ggataggcga tgttgttcca gccggtcgcc gtggtgttgt
     2281 cgcggtaata gccgcgcagc aacgtgtaga tgtagtcggt gcccttgaag tcgaacgtcg
     2341 tgcgcgcacg cgtgatcacg gaaagatcgg gcgggacctt gccgaaccag accttggcgt
     2401 ccgacggcgc catcggaatc ttcatcggat cgccgacctt ctggctaccg aaaatcagga
     2461 attccttgat ttgtttgtcg tccaggccga tctgctgcag gcgattccag cgcaccaggt
     2521 tcgcgctgtg gcagcccatg cagtagttgg caaacaggcg cgccccattt tgcagcgacc
     2581 cgaagtccct gctgcgctct gacggccagg tctcgatcgg aatcgaggcg cctgcggctg
     2641 acgccgatcc aaccgacacc agcaagccaa gcgcaatgca ggcacgggta atgatgtctc
     2701 tcattcttat ccccggccct ttcaatgcgg atggaaagtg acgcgatccg gcaccggctt
     2761 gaacgtgccc attcgactcc accacggcat cagtgcgaag aatccaaagt agatgaccgt
     2821 gccgatcatc gaggcgtact gcccggccgt cgacggcggt tgcgtgccgt aataaccgag
     2881 cacgatgaag gcgacgaccc agactatcag cagccagaag tgccagcccg ggcgatagcg
     2941 catcgacttg gccggcgact ggtcgagcca gggcagtccg agaaagatca ttacggaggc
     3001 tcccatcagc actacgcccc agaacttggc gtcgagcgtg aagtacccca gcaggagcac
     3061 cagcgtgcca accgcgatcg caccacgcac caccggattc cggagggtca tcagtattag
     3121 tgccgacagg acgatcgtgc cgacggcgat caccgccatg aactgctccg tggtcgcgcg
     3181 caggatcgag tagtacggcg tgaaatacca caccggtgcg atgtgggccg ggccctggta
     3241 cggatcggcc gggatgaagt tgttccactc gaggaagtag ccgcccatct cgggcgcgaa
     3301 gaacacgacc gcgaaaaaca ggattaggaa aatggcgagc cagaagatgt cgtgcaccgt
     3361 gtagtacgga tgaaatggga tgccatcgat cggcttgccg ctggcatcga gattcttctt
     3421 gatctccacg ccgtccgggt tgttcgagcc gacatcgtgc cgcgcgagga tgtgagcgac
     3481 aaccaggccg atcagcacga gcggtatcgc gatcacgtgg aacgcaaaga agcggttcag
     3541 cgtggcgtcc ccgatcacgt agtcacctcg cacccagacg gagagatcgg ggccgatcaa
     3601 cgggatcgcg ccaaacaggt tgatgatcac ctgggcgccc cagtagctca tctgacccca
     3661 cggcagcagg tagccgaaaa aggcctcgcc catcagacac aggaagatca ggcagccgaa
     3721 aatccatacc agttcgcgcg gggtgcggta gctgccgtac atcagtccgc ggaacatgtg
     3781 cagatagacg acgatgaaga agaacgatgc gccggtggag tgcatgtagc ggatgaacca
     3841 gccgcctggc acgtcgcgca tgatgtactc gaccgaggcg aacgcgaggt tggcgtccgg
     3901 cttgtaatgc atcaccagga agatgcccgt gatgatctgg atcgccagca ccgtgatcgc
     3961 gaggccgccg aagatgtacc agacgttgaa gttcttcggc gcgtagtact cggacaggtg
     4021 cttcttgtac tccgccatga ccgtcggcat gcgctggtcg aaccagccgg ccaggccggt
     4081 gtacggactc ttgtattcag ccatcgcgat ccccgggaat caggcgttcg tgtcgtcacc
     4141 gatcacgatg acggtttcgg acatgtactt gtgcggcggc acgacgaggt tgtccggcgc
     4201 gggcttgttc ttgaatacgc ggcccgccag atcaaaggtc gagccgtggc acgggcacag
     4261 gaagccctga tcggcaccca gctgcgcatt ctggccggga ccgaacgggc caaccggatt
     4321 gcaccccagg tgagtgcaga tgccgaccgt gatgaggaac tcgggcttgc gcgagcgata
     4381 ctcgttgcgg gcgtactccg gcgtcgggaa cgcggtgcgc agcgacttgg ggtcggcgac
     4441 ctcgccgtcc gccttcttga tcgactgcag catttcggga gtgcggcgca ggacccacac
     4501 gggcacgcca cgccactcga ccgtcttcat ttcacccggc gcgagcacgg ccaggtcgaa
     4561 ttcaacgggc gcaccggcgg ccttcgcccg ctccgacggg gcaaaggagg agacgaaggg
     4621 cacagccagc gccacggctc cggccccgcc cacggtcgcg gccactgcga tggcgttgcg
     4681 gcgttccttg tcgacgcccg cgggcatctc gccggcagga acggaggtcg atgtagacat
     4741 gaaagccccg aaattggttt gatggccgga agcccggcga tccagcgccg cggtctgatt
     4801 cctgcggtca actgcaggtc aaatccgcgc tgcgcagtag cgccccgacg gcagcccgcg
     4861 attctactgt atgtagcaag cgcgaccgcg acggcctttg aggccgaacg ctcacgatcg
     4921 agcagctaaa cttgccctag aggcaatact gccacgggag ccacgatggg catgatgaag
     4981 gaattccggg aattcgccgt caagggcaac gtcatcgacc tcgcggtcgg catcatcatc
     5041 ggggctgcgt tcggcaagat cgtcgattcg ctggtccggg atgtcgtgat gccgctcatc
     5101 aacgcggtgt tcggcggcgc ggtggacttt tccaacaagt actgggtgct gtcgggcaac
     5161 gtccccgcag gagcgtcgct cgatgcggcg cagaaaagcg gcgtcgtgtt cgcctggggc
     5221 aactttgcca ccgtgctgct gaatttcgtc atcctggcct tcgtcatctt ctggatggtc
     5281 aaggccgtca accgcgtcaa ggcgaacctc gatgccgccg cggcaccgac gccggaagaa
     5341 gtggcgctac tgcgtgagat ccgcgacgaa ctgaagcgac gctgatgttc aggtcaatgc
     5401 ggatactcag gtcacagtag ataccccttt caccgcggaa acctgaaacg atccgatgct
     5461 ccgacgtatc tggctgatct tcgcgcaggc aacgacgatc gggcttgcac tgctcttcgt
     5521 cgtttccacg ctgcgaccgg agtggctggc tcgcacgccg gcggccccgg cgca
//
LOCUS       DATIRT010000254         4548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1905234, whole genome shotgun sequence.
ACCESSION   DATIRT010000254 DATIRT010000000
VERSION     DATIRT010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1905234"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..1101
                     /locus_tag="VLN25_05875"
     CDS             64..1101
                     /locus_tag="VLN25_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSQ10126.1"
                     /translation="MNLDIALLLAQDGITNGAIYALLALALVLAFAVTRVIFIPQGEF
                     VAYGALTLVALQAGRVPGTIWLLLAAGVTVAIVEGVAAMRSREWRRYAWVALWNVAYP
                     LALWALLSAVPLTQWPMPLQILLALAIVVPLGPMVYRLAYQPLAEASVLILLIVSVAV
                     HVVMVGLGLLVFGAEGSRTPPFSEGTIQLGALTASAQSLWVIGCSLALIVVLYLFFER
                     TLYGKALRATAVNRRGARLMGIPTEFAGKLSFFLCALIGALSGILIAPITTIYYDTGF
                     LIGLKGFVGAIVGGLASYPIAAAGALLVGLLESYSSFWASAYKEVIVFTLIIPVLLWR
                     SLRTHHVEEDE"
     gene            1101..2933
                     /locus_tag="VLN25_05880"
     CDS             1101..2933
                     /locus_tag="VLN25_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HSQ10127.1"
                     /translation="MNSVRSHWFVAVFLAALALAPLVLPEFWITLANYIGLYALVALG
                     LVLLTGVGGLTSFGQAAFVGLGAYATAVLTTVQLPAGLAWLGASPWLTLLVGFAITLV
                     VAAALGSLTLKLSGHYLPLGTIACGISLSLLFGTSELLGGYTGLTGVPPITIFGISLE
                     TGRSIYYLIWAFLLAGVFTSRNMLDSREGRAIRALKGGMVMAESMGVNTSASRMVAFV
                     LAALMACASGWLYAHMQRFVNPTPFSLHVGIEYLFMAVIGGAGYVWGALLGAAVITIS
                     KTWLQDILPHIVGQAGNFEVIVFGILMIVILQRAPDGLWPVITRRFQVRPSRRVIAPE
                     VPPLARRPVPTAGELLLEVQGATKRFGGLVAVNAMDLRVNAGQIVALIGPNGAGKSTM
                     FNLISGVLDATAGSFRFRGFDVAGRSSREIARLGLSRTFQHVRQLPQMSVVDNVAIGA
                     HRREYPGLLAGVFGGAWRLDRRDEAKLLAEAARQIERVGLADHMFDAAGSLPLGKQRI
                     LEIARALASDPCLLLLDEPAAGLRYLEKRALADLLRKLRAEGLSILLVEHDMDFVMGL
                     VDLVTVMDFGQKIAEGRPDEVQRDPRVLEAYLGGIDETDAVAQG"
     gene            2941..3684
                     /locus_tag="VLN25_05885"
     CDS             2941..3684
                     /locus_tag="VLN25_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSQ10128.1"
                     /translation="MTTRLLEVRDLSVTYGQVAALAQANLDVDAGRIVTVIGPNGAGK
                     TTLLAAIMGALPTNGAARGIVRFAGDDGKRASLEQRVARGMTLVPEKRELFGEMTVED
                     NLLLGAFQRYRSGQRDHAQTMEEVFTLFPRLLERRTQLAGTLSGGERQMLAIGRALMA
                     RPTLLMLDEPSLGLAPRIVREIFHIIVELRRRGVSILLVEQNARAALQIADHAYVLET
                     GEVVLDGPANQLAHDPRVIESYLGLGQKH"
     regulatory      3718..3813
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3868..>4548
                     /locus_tag="VLN25_05890"
     CDS             3868..>4548
                     /locus_tag="VLN25_05890"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HSQ10129.1"
                     /translation="MSANPKFIAADARVDEAAVQPLPRSRKIYVPGSRTDVRVPMREI
                     TQSNTPTTMGAEVNPPLTVYDTSGPYTDPTVAIDIRSGLAALRAPWIAEREDTEALAG
                     PSSRFGVERLNDPKLGELRFNLHRKPRRAKPGANVSQMHYARRGIVTPEMEYIAIREN
                     ARANQRAEQHSEMLRRQHPGQSFGANLPALITPEFVRDEVARGRAIIPANINHPELEP
                     MIIGRNFLV"
BASE COUNT          688 a         1438 c         1562 g          860 t
ORIGIN      
        1 ataagcaaaa gggctagtcg cgagccgccc agtcgcgcat gcagtcagta gactgaaagc
       61 acgatgaact tagacatcgc ccttctgctc gcgcaggacg gcatcaccaa cggcgcgatc
      121 tatgcgctgc ttgcgctcgc gctggtgctg gcgtttgcgg tcacgcgggt gatcttcatt
      181 ccgcagggcg agttcgttgc gtatggtgcg ctgacgctgg ttgcattgca ggcggggcgg
      241 gtgccgggaa cgatctggct gctgctggct gcaggtgtca cggtcgcgat cgtcgaaggg
      301 gtcgcagcga tgcgctcgcg ggagtggcga cgttacgcct gggtggcgtt gtggaatgtc
      361 gcgtatccgc tggcgctttg ggcgctgctg agcgccgtgc cactgacgca atggccgatg
      421 ccgctgcaga tcctgctggc gctcgcgatc gtcgtgccgc tcgggcctat ggtgtatcgc
      481 ctggcgtacc agccgctggc ggaggcgtcg gtgctgatcc tgctgatcgt ctcggtcgcg
      541 gtgcatgtgg taatggtcgg tctcggcctg ctggttttcg gggccgaggg ttcacgcaca
      601 ccgccgtttt cggaaggcac catccagctg ggtgcgctga ccgcgagtgc acagtcgctg
      661 tgggtgatcg gctgttcgct ggcgctgatc gtggtgctgt atctgttctt cgagcgcacg
      721 ctgtacggca aggcgctgcg cgcaacagct gtcaatcgcc gcggcgcccg gttgatgggc
      781 ataccgacgg agttcgcggg caagctctcg ttcttcctct gtgcgctgat cggcgcgctg
      841 tcgggcatcc tgatcgcgcc aatcacgacg atctactacg acaccggatt cctcatcggg
      901 ttgaagggct tcgtcggcgc gatcgtcggt ggcctggcca gctatccgat cgctgcggcc
      961 ggggctttgc tggtcggcct gctcgagtcg tactcgtcgt tctgggccag cgcctacaag
     1021 gaagtgatcg ttttcacgct gatcattccg gtgctgctgt ggcggtcgct gcgcactcat
     1081 cacgtcgagg aggacgagtg atgaactcgg tgcgatcgca ctggtttgtc gcggtgttcc
     1141 tggcggcgct ggcgttggct cccctcgtgt tgcccgagtt ctggatcacg ctggcgaact
     1201 acatcggcct gtatgcactg gtggcgcttg gcctggtgct gctgaccggg gtcggcggcc
     1261 tgacatcgtt cgggcaggcg gcgtttgtcg gcctgggcgc ctatgcgacg gccgttctga
     1321 caaccgtcca gctgcccgcc gggctggcct ggcttggcgc atcaccctgg ctgaccctgc
     1381 tggtgggctt cgcgatcacg cttgtcgttg cggcggcgct cggttcgctg acgctgaagc
     1441 tgtccggtca ctatcttccg ctcggcacga tcgcgtgtgg catcagcctg tcgctgctct
     1501 ttggcacatc ggagctgctc ggcggctata ccgggctgac gggtgttccg ccgatcacga
     1561 tcttcggcat ctcgctggaa acgggacgca gcatctacta cctgatctgg gccttcctgc
     1621 tcgccggcgt gttcacttcg cgcaacatgc tcgattcgcg cgaaggccgc gcgatccgtg
     1681 cgctgaaggg cgggatggtc atggccgagt cgatgggcgt gaacacgtcg gcctcgcgca
     1741 tggtggcgtt cgtcctcgcc gcgctgatgg cgtgtgcgtc ggggtggctc tacgcgcaca
     1801 tgcagcgctt cgtgaatccg acaccatttt cgctgcatgt cggtatcgag tacctgttca
     1861 tggccgtgat cggaggggcg ggctacgtct ggggggcgtt gctcggcgct gccgtgatca
     1921 cgatctcaaa gacctggctg caggacatcc tgccgcatat cgtcgggcag gcgggcaact
     1981 tcgaggtcat cgtgttcggc atcctgatga tcgtcatcct gcagcgcgcg ccggacgggc
     2041 tgtggccggt gatcacgcgg cgctttcagg tgcggccgtc gcgacgcgtg attgcgccgg
     2101 aggttccccc actggcgcgc cgcccggtgc cgacagccgg cgagttgctg ctcgaagtgc
     2161 agggggcgac caagcgcttc ggcgggctgg tcgccgtcaa cgccatggac ctgcgcgtca
     2221 atgccggcca gatcgtcgcg ctgatcggcc ccaacggcgc gggcaagagc acgatgttca
     2281 acctgatttc aggcgtgctc gatgcgacgg cggggagttt ccgcttccgc ggctttgatg
     2341 tcgccggacg ctcgtcgcgc gagattgcgc gactgggact gtcgcgcacg ttccagcacg
     2401 tgcgacagct gccgcagatg tcggtcgtcg acaacgtggc gatcggtgcg caccgacgcg
     2461 agtaccccgg gctgctcgcc ggcgtgtttg gcggcgcgtg gcggctggac cgccgcgacg
     2521 aggccaagct gctggccgag gcagcgcgcc agatcgaacg cgtgggactt gccgatcaca
     2581 tgttcgatgc ggcggggtcg ctgccgctcg gcaagcaacg catcctggag attgcacgcg
     2641 cgctggcatc cgacccctgc ctgctgttgc tggacgagcc ggcggccggc ttgcgctacc
     2701 tcgaaaagcg cgcacttgct gatctgctgc gcaagctgcg tgcggagggc ctgtcgatcc
     2761 tgctggtcga acacgacatg gacttcgtga tgggactggt cgatctcgtc acggtgatgg
     2821 atttcggtca gaagattgcc gaaggacggc ccgacgaagt gcagcgcgac ccgcgcgtgc
     2881 tcgaggccta tctgggcggc atcgacgaga ccgacgcggt ggcgcaggga tgaccacgag
     2941 atgaccacgc gcctgctcga agtgcgcgac ctgtccgtga cctatggaca ggtggctgcg
     3001 ctcgcccagg cgaacctcga tgtcgacgcc ggccgcatcg tcacggtgat cggtccgaac
     3061 ggcgccggca agaccacatt gctggcggcg atcatggggg ccctgccgac caacggcgcg
     3121 gcccgcggca tcgtgcgttt tgccggtgac gatggaaagc gcgcctcgct cgagcagcgc
     3181 gtcgcacgcg ggatgacgct ggtacccgaa aagcgtgagc tcttcggcga gatgacggtc
     3241 gaggacaacc tgctgctcgg ggcgttccag cgctatcgct ccggacagcg ggaccatgca
     3301 cagacgatgg aggaagtgtt cacgctgttc ccgcggttgc ttgaacgccg cacgcaactg
     3361 gccggcacgt tatccggtgg cgaacggcag atgctcgcca tcgggcgggc gctgatggcc
     3421 cgaccgacgc tgctgatgct cgacgagcct tcgctgggcc tggcaccacg catcgtacga
     3481 gagatctttc acatcattgt cgaactgcgc cggcgtggcg tgtcaatcct gctggtcgag
     3541 cagaacgcgc gggcggcgct gcagattgcc gaccatgcct acgtgctcga gaccggagaa
     3601 gtcgtgctcg acggcccggc caatcagctg gcgcacgatc cgcgcgtcat agagagctat
     3661 ctcgggctcg gccagaaaca ctgagccgcg gccgccggcc ccggtggtgt atcgtcatca
     3721 gcgcgttggg ggtatcgcgg cgatcgcgat tgagacagtc ccctcgaacc tgaaccggtt
     3781 gagaccggcg gagggaaacg cgccagccat tgtggcgtcg tcgtgccctc cgccgtcgtt
     3841 cctacggaac ctgcaaggag tcgacgaatg agtgccaatc ccaagttcat tgcagcggac
     3901 gcgcgagtcg acgaggccgc cgtccagccg ctgccccgct cccgcaagat ctacgtgccg
     3961 ggctcgcgca ccgacgtccg ggtgccaatg cgcgagatca cgcagagcaa cacgccgacc
     4021 acgatgggcg ccgaggtgaa tccgccgctg acggtgtacg acacgtcggg gccctacacc
     4081 gacccgacgg tcgcaatcga catccgctcc gggctggccg ccctgcgcgc gccctggatt
     4141 gccgagcgcg aagacaccga ggcgctggcc ggtccgagct cgcgctttgg cgtggagcga
     4201 cttaacgatc cgaagctcgg cgagctgcgc ttcaacctgc accgcaagcc acgccgcgcc
     4261 aagcccggtg ccaacgtgtc gcagatgcac tatgcccgtc gcggcatcgt cacgcccgag
     4321 atggagtaca tcgccattcg tgaaaatgcg cgcgcgaatc agcgtgccga gcagcattcg
     4381 gagatgctgc gccgtcagca tcccggccag agcttcggtg caaacctgcc tgcgctcatc
     4441 acgcccgagt tcgtgcgtga cgaagtggcg cgcggacggg ccatcatccc cgccaatatc
     4501 aatcacccgg aactggaacc gatgatcatc ggccgcaact tcctggtg
//
LOCUS       DATIRT010000255         3646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_529046, whole genome shotgun sequence.
ACCESSION   DATIRT010000255 DATIRT010000000
VERSION     DATIRT010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529046"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VLN25_05895"
     CDS             complement(<1..279)
                     /locus_tag="VLN25_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HSQ10130.1"
                     /translation="MTQHFDSRETRDPRQRELDLMGHLPGLVANALKASGWRQHLGSI
                     DPATIVTRADLARLPVLRKSELPALQKAAPPFGGLIADPIERFGRLFTS"
     gene            complement(303..476)
                     /locus_tag="VLN25_05900"
     CDS             complement(303..476)
                     /locus_tag="VLN25_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10131.1"
                     /translation="MSALVSLALVGGCTPRADESSTPLPTRQTTIDPVVKKLDAAQQD
                     AERRRAEIDGAGK"
     gene            complement(585..956)
                     /locus_tag="VLN25_05905"
     CDS             complement(585..956)
                     /locus_tag="VLN25_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10132.1"
                     /translation="MATAKKAAKKKSRRTVMYSTGGKKLYAVRDATGKFKDIQQYSRA
                     HAMDIKRGSSAEATAEAKKAAKKVAKKAAKKGGAKKATRKSTVKKVAKKAVKRVTTAR
                     KAANQLVKRLRSAVARVRAKL"
     gene            complement(1064..1885)
                     /locus_tag="VLN25_05910"
     CDS             complement(1064..1885)
                     /locus_tag="VLN25_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSQ10133.1"
                     /translation="MTALLLNVNGIEVIYNHVILVLKGVSLTVPEGRIVALLGANGAG
                     KTTTLRAVSNLLHGERGEVTKGSIEYRGEKIERLTAYDLVKRGVVQVMEGRRCFAHLT
                     VEENLLSGAYSRGASGRGNSRAEIAADLDKVYAYFPRLKQRRTSQSGYTSGGEQQMTA
                     IGRALMARPSMILLDEPSMGLAPQVVEEIFAIVQDLNKKEQVSFLLAEQNTNIALRFA
                     DYGYILENGRVVMDGDASALRENEDVKEFYLGVSKAGRKSFREAKFYKRRKRWLA"
     gene            complement(1977..3416)
                     /locus_tag="VLN25_05915"
     CDS             complement(1977..3416)
                     /locus_tag="VLN25_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSQ10134.1"
                     /translation="MTTFLKARVAAAVVAGVAAAVSLALPVSAGAQQKAAPAAGKGDQ
                     FFPVLVYRTGAYAPNGTPWANGYVDYLKLVNAQGGINGVKITWEECETGYATDRGVEC
                     YERLKTKPFVSAVQPLSTGITFALTDKAPTDKIPLITAGYGRSESVEGEAFPWNFPLM
                     GTYWDAADVLVQHVGKLNGGTDKLKGKKIALVYHDSPFGKEPIALLQERAKLHGFELM
                     TIPVTHPCVEQKAAWLQIRQNRPDYVFLWGWGVMNSTALREAVATGYARDKMYGVWWA
                     AAEPDVTPVGADAKGYNGLALQHGAGRAKMHEDILKLVHDKGQGTGPKDEVGSVLYTR
                     GMISAMLQVEGVRQAQLRYGAKPLTGEQVRYGLENLNLDAKRLEALGLTGVMKPLATN
                     CRDHAGARSARIQQWDGTKWVFTSDFYEADNQIIKPMIKAAAQRYVTDKKLTMRSCAD
                     EIAADVKSASAAAAPAAVKTAPAPAPAKK"
     gene            complement(3476..>3646)
                     /locus_tag="VLN25_05920"
     CDS             complement(3476..>3646)
                     /locus_tag="VLN25_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSQ10135.1"
                     /translation="QVPGTIGIEMSTALASHLELMIFGSLIVFFLIVEPHGLARLWAI
                     GKEKLRLWPFPH"
BASE COUNT          592 a         1237 c         1070 g          747 t
ORIGIN      
        1 cgaagtgaac agccgcccga aacgctcgat cggatcagcg atcaggccac cgaagggcgg
       61 tgccgccttc tgcagcgctg gcaactcgga cttgcgcagc accggcaggc gcgcaagatc
      121 ggcacgcgtg acgatggtgg caggatcgat ggaccccagg tgctggcgcc agcccgatgc
      181 cttcagcgcg ttggcaacca gccccggcag gtgccccatc agatcgagtt cgcgctggcg
      241 gggatcgcga gtctcgcgac tgtcaaagtg ctgcgtcatg cgaacctcgc ctgtgtatcc
      301 gcttacttcc ccgcgccatc gatttctgca cgacgacgtt ccgcgtcctg ctgggcggca
      361 tccagcttct tgacgaccgg atcgatggtt gtctgcctcg tcggcagcgg tgtcgacgac
      421 tcgtcggcgc gaggagtgca gcctccgacc agcgcgaggg aaaccagcgc cgacatcaat
      481 gcgggtcctt gcgctctcat cggggtcggg atcatcacag caggcatcca cgcggcaggg
      541 ccgggcgcgc agcgagcccg gcacctttcc tgtcggaatc gcgactacag ctttgcgcgc
      601 accctggcca cggcgctgcg caggcgcttc accagctggt tcgcggcctt cctggctgtc
      661 gtcacccgct tgaccgcttt cttcgcaacc ttcttcaccg tcgacttgcg cgtcgccttc
      721 ttggctccgc ccttcttcgc ggccttcttc gcaaccttct tcgctgcttt tttcgcttca
      781 gccgttgcct cggcgctgct accgcgcttg atgtccatgg cgtgcgcacg cgagtactgc
      841 tggatgtcct tgaacttgcc ggtcgcgtca cgcacggcat acagcttctt gccacccgtg
      901 ctgtacatca ccgtacggcg ggacttcttc ttcgcagctt tcttggctgt ggccatctat
      961 gctccttgag gattgcgcgg ccccgaactc gctttcgcca acccgcgcgc gctggcgaaa
     1021 acccccgcgc atctctgcgc ggaaattaaa tgcgccgcgc gcttcaggcg agccagcgtt
     1081 tgcgtcgctt gtagaacttg gcctcccgga aactcttgcg gccggccttc gacacgccga
     1141 gatagaactc cttcacgtcc tcgttctcgc gcagcgccga tgcatcgccg tccatgacga
     1201 cccggccgtt ttccaggata tagccgtagt cggcaaagcg cagcgcgatg ttggtgttct
     1261 gttccgcaag caggaacgac acctgctcct tcttgttgag atcctgcacg atcgcaaaga
     1321 tctcctcgac cacctgcggc gccaggccca tcgacggctc gtcgagcagg atcatcgatg
     1381 ggcgtgccat cagcgcgcgc ccgatcgccg tcatctgctg ttcgccgccc gacgtatagc
     1441 ccgactggct ggtgcgccgc tgcttcaggc gcggaaagta ggcatagacc ttgtcgagat
     1501 ccgcagcgat ttcagcccgc gagttgccgc gtcccgatgc tcccctcgaa tacgcgcccg
     1561 acagcaggtt ttcctccact gtcaggtggg cgaagcagcg ccgtccttcc atcacctgca
     1621 cgacaccgcg cttgaccagg tcgtaggcgg tcaggcgttc gatcttctcg ccgcggtatt
     1681 cgatcgaacc tttggtgact tcgccgcgct cgccgtgcag taggttggat accgcgcgca
     1741 gggtcgtggt ctttcccgcg ccgttggcgc cgagcaacgc gacgatgcgg ccttcgggca
     1801 cggtcagcga gacccccttc agaacgagga tcacgtggtt gtagatgact tcgatgccgt
     1861 tgacgttgag cagcaaggct gtcacgttaa ctccatagtc actgtgcgaa gggctcacgc
     1921 agtaagcact tcgcacagcc ggccgcacta cgcgaccggc tatgtcgaac ctggcgctac
     1981 ttcttggccg gtgccggcgc cggcgccgtt tttaccgccg ccggagcagc tgcagcggag
     2041 gcagacttca catctgcggc aatctcgtcc gcgcagctgc gcatcgtcag tttcttgtcg
     2101 gtgacatagc gctgcgctgc ggccttgatc atcggtttaa tgatctggtt atccgcctcg
     2161 tagaagtccg aggtgaaaac ccacttggtg ccgtcccatt gctggatgcg cgccgaacgg
     2221 gctccggcat ggtcgcgaca attcgttgcc agcggcttca tcactcccgt caatccgagc
     2281 gcttcgagcc gcttggcgtc gagattcagg ttttccaggc cgtagcgaac ctgctctccg
     2341 gtcagcggct ttgccccgta ccggagctgg gcttgacgca cgccttcgac ctggagcatc
     2401 gcgctgatca tgccgcgcgt gtacagcacg ctgccgactt cgtccttcgg ccccgtcccc
     2461 tggcccttgt cgtgcacgag cttcaggatg tcctcgtgca tcttggcccg accggctccg
     2521 tgctgcagcg ccagtccgtt gtagcctttc gcgtccgcac ccaccggcgt cacatcgggc
     2581 tcggccgcgg cccaccaaac gccgtacatc ttgtcgcgcg catatccggt cgccacagcc
     2641 tcccgcagtg cagtggagtt catcacaccc cagccccaca ggaacacgta gtccggacgg
     2701 ttctgtcgga tctgcagcca tgcagccttc tgttcgacgc acggatgcgt gaccggaatc
     2761 gtcatcagtt caaagccatg cagcttcgcg cgctcctgaa gcagcgcaat cggctccttg
     2821 ccaaagggcg agtcgtgata gacgagcgca atcttcttgc ccttgagctt gtcggtaccg
     2881 ccgttcagtt tgccgacatg ctggaccaga acatcggccg cgtcccagta ggtccccatc
     2941 agcgggaagt tccacgggaa cgcttcgcct tcgaccgatt ccgagcggcc atagccggcc
     3001 gtaatcagcg gaatcttgtc ggtgggggcc ttgtcggtca gagcaaaggt aatgccggtg
     3061 gacaagggct ggaccgcgga gacgaaaggc ttggtcttca ggcgctcgta gcactcgacc
     3121 ccgcggtcgg tcgcgtaacc ggtctcgcac tcttcccagg tgatcttcac gccgttgatg
     3181 ccgccctgcg cattgaccag tttcaggtag tcaacgtagc cgttggccca tggcgtgccg
     3241 ttgggcgcat aggcaccggt ccggtaaacc aacacgggga agaactggtc tcctttcccg
     3301 gctgcgggag ccgccttctg ctgcgcgccc gcgctcaccg gcagcgccag cgacactgcc
     3361 gccgccaccc cggcgacgac cgccgcagcg actcttgcct tcaaaaacgt tgtcatcgat
     3421 gtctccatac gtgaagggtc cagcgttatc ttgccgcgcc gtttctccgt ggacctcagt
     3481 gcgggaacgg ccatagtctc aatttctcct tgccgatcgc ccacagccgc gcaaggccgt
     3541 gcggttcgac gatcaggaag aacacgatca ggctgccgaa gatcatgagc tccagatgcg
     3601 aagcgagcgc cgtcgacatt tcaattccga tggttcccgg aacctg
//
LOCUS       DATIRT010000256         3147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1022132, whole genome shotgun sequence.
ACCESSION   DATIRT010000256 DATIRT010000000
VERSION     DATIRT010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022132"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VLN25_05925"
     CDS             complement(<1..326)
                     /locus_tag="VLN25_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HSQ10136.1"
                     /translation="MRFSVYQESKKGGRRVNQDRMGYLYTRDSMLVLVADGMGGHARG
                     EVASQVTLQTIASIYQRDAKPILPDPTRFLEESVLASHRELHRYRAEHSLPEAPRTTL
                     VACIVQ"
     gene            complement(456..1451)
                     /locus_tag="VLN25_05930"
     CDS             complement(456..1451)
                     /locus_tag="VLN25_05930"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HSQ10137.1"
                     /translation="MAAQTNASLPEGLEVGGYRIVKKISSGGFSIVYLATDENGNSVA
                     IKEYMPAALAQRKPGELSPYVSPENLNTYRIGLKCFFEEGRALASIYHPNIVRVINFF
                     RANDTVYMVMNYESGRSLQEHVLRNRNKDQKDVLSERFVRRVFAQVMNGLREVHSNRL
                     LHLDVKPANVYLRMDGTPILLDFGAARQTLQRDISKTYPMYTPGFAAPELYKRDAELG
                     PWTDVYSIGSCIYACMSGMPPQESDQRLKEDKMPTALRSFRGLYSNQLISMVERCLRL
                     NSLERPQTVYAVQKELLQNVPERPEFSIMERTGARWRSLQDRMMRFMKEHNITWF"
     gene            1587..2489
                     /locus_tag="VLN25_05935"
     CDS             1587..2489
                     /locus_tag="VLN25_05935"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HSQ10138.1"
                     /translation="MKPVASMTGFAVASRPTVIGPVTVELKSVNSRFLDLSVRYPDEL
                     RSVEPALREAISARVNRGKMECRLAVSRVAGESPGGVNTAALGRLKQLAAEVVAVVPF
                     ASPLTTADVLGWPGVVDAPVADPDALRVAVLDALSEALDALAASRQREGSALRDVLHA
                     HCNAIDVIGAQVKARAPELLAAVERKLVERLEQALGRALADTSTLTREEVADRIRQEV
                     TLYGLRMDVDEELKRLTTHVGEVRRVLDAGGPVGRRLDFLMQELNREANTLGSKAAAI
                     EMTNASVELKLLIEQMREQIQNLE"
     gene            2486..>3147
                     /gene="gmk"
                     /locus_tag="VLN25_05940"
     CDS             2486..>3147
                     /gene="gmk"
                     /locus_tag="VLN25_05940"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HSQ10139.1"
                     /translation="MSTSSVTPRAALDAGKAGSIFLVVAPSGAGKSTLVNALLDQDAR
                     IELSVSHTTRPPRPGEADGREYHFVSVDEFMRRRTAGEFLESAEVHGNYYATSRVWIE
                     ERLRAGTDVLLEIDWQGARQVKSHFGHAVGIFILPPSIDALEARLHKRGQDSPQVITR
                     RLLAAGSEIAHASEFDYVIINEDFPIALAQLSAIVTATRQRYARQAARHHELFAQLGI
                     ALP"
BASE COUNT          577 a         1004 c          979 g          587 t
ORIGIN      
        1 tgctggacga tgcacgcgac cagcgtcgtc cgtggtgcct cgggaaggct gtgctcggct
       61 cgatagcgat gcagctcgcg gtgcgaagcg agcacggatt cctcgagaaa gcgcgtcggg
      121 tccggcagga tcggcttggc gtcgcgctgg tagatcgacg cgatcgtctg cagcgtcacc
      181 tgcgaggcga cctcgccgcg cgcatggccg cccatgccgt cggccaccag gaccagcatc
      241 gagtcgcgcg tgtacaggta gcccatgcgg tcctgattga cccgccggcc gcccttcttg
      301 ctttcctggt aaaccgaaaa acgcatcagc gctccatcac accggaggca cagtgccagc
      361 ttttgccgcc tttgtaatga gaaaaatcga ctggctccgc cagcgatttt ttcacttcag
      421 ccgcgcaaaa gtaggggtgc actcttgcgt gtttctcaaa accacgtgat gttgtgttcc
      481 ttcatgaacc tcatcatccg gtcctgaagg ctgcgccagc gggcacccgt gcgctccatg
      541 atcgaaaact ccggccgttc cggcacgttc tgcaggagtt ccttctgcac cgcatagacc
      601 gtctgtggtc gctcgagcga gttcaggcgc agacagcgtt cgaccatcga gatcaattgg
      661 ttcgaataaa ggccgcggaa cgaccgcagc gccgtgggca tcttgtcttc cttcaggcgc
      721 tggtccgact cctgcggcgg catgcctgac atgcaggcgt atatgcacga accgatcgag
      781 tacacgtcgg tccacggacc cagctccgcg tcgcgcttgt acagttcggg ggcagcaaat
      841 cccggggtgt acatcggata agtcttgctg atgtctcgct gcagggtctg ccgtgctgcg
      901 ccgaagtcca gcaggatagg cgtgccgtcc atgcgcaagt agacattcgc aggcttcacg
      961 tcgaggtgaa gcaggcggtt cgagtgcact tcgcgcagcc cgttcatgac ctgcgcaaaa
     1021 acgcgtcgga cgaacctttc ggacagcacg tccttctggt ccttgttgcg gttgcgcagg
     1081 acatgctcct gcagcgaccg tccggactcg tagttcatca ccatgtacac cgtgtcgttc
     1141 gcgcggaaga aattgatgac gcgcacgata ttggggtgat agatcgacgc cagtgcccgg
     1201 ccttcctcga agaagcactt caggccgatg cgataggtgt tcaggttctc cggagagaca
     1261 tagggcgaca gttcgcccgg cttgcgctgc gcgagcgccg cgggcatgta ctccttgatc
     1321 gcgaccgaat tgccgttctc gtccgtggca aggtagacga tggagaagcc gccgctggag
     1381 atcttcttta caatgcggta accgccgacc tcgaggcctt cgggcaaaga tgcgttggtc
     1441 tgcgcggcca tgtgcactac tgcgtgggaa tatcgggacg attctaacga aaagggttcc
     1501 gtgtcccgaa gccttcatag ggcagaaaaa caccgctata ccgagccttc gagcgagtcc
     1561 gttatcggca ttcctccaac atccgcatga aacccgttgc cagcatgacc ggatttgcgg
     1621 tcgccagtcg acccaccgtg atcggtccgg tcactgtcga actgaagtcc gtcaactcgc
     1681 gtttccttga cctgtcggtc aggtatccgg acgaactgcg cagcgtcgag ccggcattgc
     1741 gggaagcaat cagcgcacgt gtcaatcgcg gcaagatgga gtgccgcctc gccgtcagtc
     1801 gcgttgccgg tgaatcccct ggcggtgtca acaccgcggc cctgggtcgc ctcaagcaac
     1861 tggcggccga agttgtcgcc gtggttccat tcgcgagccc cctgacgacc gcggacgtac
     1921 ttggctggcc cggtgtcgtc gacgcgccgg tcgccgaccc ggatgcgctg cgcgttgccg
     1981 tcctggatgc cctgtccgag gcgctcgatg cgctggccgc ctcgcgccag cgggagggct
     2041 cggcgctgcg ggatgtgctg cacgcacact gcaatgccat cgatgtgatt ggcgcgcagg
     2101 tcaaggcccg ggcacccgag ctgctcgcgg cggtcgagcg caaactggtc gagcgactgg
     2161 agcaagccct gggacgcgcg ctggccgaca cttcgacgct gacccgcgag gaggtcgccg
     2221 accgtattcg tcaggaagtc accctttacg gcctgcgcat ggacgtcgac gaggaactca
     2281 agcgcctgac gacacacgtc ggcgaagtgc gccgtgttct cgacgccggg ggaccggtcg
     2341 ggcggcgcct cgacttcctg atgcaggaac tcaatcgcga ggccaatacg ctcggttcca
     2401 aggctgccgc gatcgagatg acgaacgcct ccgtcgaact gaagctgctg atcgagcaga
     2461 tgcgagagca gatccagaac ctcgaatgag cacgagttcc gtcactccgc gcgcagcgct
     2521 cgatgccggc aaggccggca gcatcttcct ggttgtcgcg ccctcgggcg cgggcaagtc
     2581 gacgctggtc aacgccctgc tcgaccagga tgcgcgcatc gagctgagcg tttcgcacac
     2641 gacgcgtccg ccccggcccg gagaagccga cggtcgcgag tatcacttcg tttcggtcga
     2701 tgaattcatg cgacggcgca cagcgggcga gtttctcgag agcgccgagg tgcacggcaa
     2761 ctactacgcg acctctcgcg tctggatcga ggaacgactc cgggccggaa ccgacgtgct
     2821 gctcgagatc gactggcaag gggctcgcca ggtcaagtcg cacttcggcc atgcggtcgg
     2881 catcttcatc ctgccgccgt cgatcgatgc gctcgaggcg cgcctgcaca agcgcggaca
     2941 ggacagtccg caggtaatca cgcgtcgctt gctggcagcg ggcagcgaaa tcgcccatgc
     3001 ctccgaattc gattacgtca tcatcaacga agacttcccg atcgcgctgg cgcagctgtc
     3061 agccatcgtc actgcaacgc gacagcgcta cgcccgacag gccgcacgcc accacgaact
     3121 ctttgcgcag ctcggaatcg cattgcc
//
LOCUS       DATIRT010000257         2914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1218887, whole genome shotgun sequence.
ACCESSION   DATIRT010000257 DATIRT010000000
VERSION     DATIRT010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218887"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1919)
                     /locus_tag="VLN25_05945"
     CDS             complement(<1..1919)
                     /locus_tag="VLN25_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HSQ10140.1"
                     /translation="MADDSKPVSADTRRAIRIRGARQNNLANLDLDIPTGELVVVTGV
                     SGSGKSSLVFDTLYAEGQRRYVETFSPYARQFLDRMDRPAVDRIEGVPPAIAIDQTNP
                     VRTSRSTVGTMTELNDHLKLLFARAARLYCRGCAQPVRRDAAESIVATISERAATAGD
                     PRLLVTFPVRVPKNFSEAEVRAHLEAQGYTQVHGERMQGDAKVLDVIADRFRATVERS
                     RIADAVERSLRLGGGRVDVRALDDAGAAAAIWRFSTDLHCADCDIHYETPHPSTFSFN
                     SPLGACETCRGFGRVIGVDYGLVIPDERKTLRGGAIKPFQTKSFIESQRDLEKCAAKD
                     GIPLDVPFRELTDAQKKWVIDGGTGWKSWKKSWPGIWYGVKRFFEWLESKAYKMHIRV
                     LLSRYRSYTPCPVCAGSRLRPDATLWRVGANEEAQEAIPSGGRYARFRPVHASWSDQQ
                     LTSLPGLSLHDLMLLPIERVKRFFDAVTLPPPLDEATEMLLDELRARLAFLVDVGVGY
                     LTLDRQSRTLSGGEVQRINLTTALGTSLVNTLFVLDEPSIGLHPRDMHRVVEVMHRLR
                     SAGNSLVVVEHDPQVMLAADRVIDMGPGPGERGGRIVFNGTPDELTHAETLTGRYLGG
                     RQSVDATAPLPVPANA"
     gene            complement(2283..2789)
                     /locus_tag="VLN25_05950"
     CDS             complement(2283..2789)
                     /locus_tag="VLN25_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10141.1"
                     /translation="MIRLLCRPLLAALVLGLLSGCATLSGEPTQKVNIQTVDAEGKAI
                     DGMSCRASNTSAKYVGVTPMFDMHVRRSSSALIVECRRDGYPLSRAVVVSRAVNMQLL
                     QMLLPGGSSLMVVDHLTGYMYAYPRWVRLQVGTDMVFDRRDEVDRGPTPGLITRQFDD
                     FVRFASTP"
BASE COUNT          496 a          948 c         1000 g          470 t
ORIGIN      
        1 gcattcgcgg gcacgggcag cggtgccgtc gcatcgaccg actgccgccc gccgagataa
       61 cgcccggtca gcgtttccgc gtgtgtcagt tcgtcgggag tgccgttgaa gacgatgcgg
      121 cccccgcgtt ctccgggccc gggccccatg tcgatcacgc gatcggcagc gagcatcacc
      181 tgcggatcgt gctcgaccac caccagtgaa ttgcccgcgc tgcgcagccg gtgcatcact
      241 tcgaccacgc ggtgcatgtc acgcgggtgc agtccgatcg acggctcgtc gagcacgaac
      301 agcgtgttga ccagcgatgt gccgagcgcc gtggtcaggt tgatgcgctg gacctcgccg
      361 cccgacagtg tgcggctttg ccggtccagc gtcaggtagc cgacgccgac atcgaccagg
      421 aatgcgagcc gcgcccgcag ctcgtcgagc agcatttcgg tggcctcgtc gagcggcggc
      481 ggcagcgtga ccgcatcgaa aaatctcttg acgcgctcaa tcggcagcag catcaggtcg
      541 tgcagtgaga gacctggcaa ggacgtgagt tgttgatccg accacgacgc atgcaccggc
      601 cgaaacctcg cgtagcgtcc gccggacgga atcgcctcct gtgcttcctc gttcgcgccg
      661 acccgccaga gcgtcgcatc cggccgcaag cggctgcccg cgcacaccgg gcagggggtg
      721 tacgaacggt agcgcgagag cagcacgcgg atgtgcatct tgtaggcctt gctctcgagc
      781 cactcaaaga agcgcttgac gccgtaccag atgcccggcc aactcttctt ccagctcttc
      841 caaccggtgc cgccgtcgat gacccacttc ttctgggcgt cggtcagctc gcggaacggc
      901 acgtcgagcg ggatgccatc cttcgccgcg cacttctcga gatcacgctg gctctcgatg
      961 aacgacttgg tctggaacgg cttgattgcg ccgccccgca acgtcttgcg ctcgtcggga
     1021 atcaccagcc cgtagtcgac accgatcacg cggccgaagc cgcggcaggt ttcgcaggcg
     1081 ccgagcgggg agttgaacga gaaagtcgac gggtgcggcg tctcgtagtg gatgtcacag
     1141 tcggcacagt gcaggtcggt agagaagcgc cagatcgctg ccgctgcgcc cgcatcgtcg
     1201 agcgcacgga catcgacgcg gccaccgccc agccgcagcg aacgctcgac cgcgtcggcg
     1261 atgcgcgaac gctccaccgt tgcgcggaac cggtcggcaa tgacgtccag taccttcgca
     1321 tcgccctgca tgcgctcgcc gtggacctgg gtgtagccct gggcttcgag gtgcgcacgg
     1381 acttcagcct cgctgaaatt cttcggcacg cgcaccggga aggtgaccag gaggcgcggg
     1441 tcgccagccg tcgcggcccg ctcggaaatg gtcgcgacga tcgactcggc cgcgtcgcgt
     1501 cggaccggct gtgcacagcc gcggcagtag agccgggccg cacgcgcgaa cagcagcttc
     1561 aaatggtcgt tgagttcggt catcgtgccg accgtgctac gcgaggtgcg taccggattg
     1621 gtctggtcga tcgcgatcgc cggcggcacg ccctcgatcc ggtccactgc gggccggtcc
     1681 atgcggtcga ggaactggcg tgcatagggc gaaaacgtct cgacgtagcg gcgctggccc
     1741 tcggcataga gggtgtcgaa caccagcgag ctcttgccgg agccggacac gcccgtgacc
     1801 acgaccagct cacccgtcgg gatgtcgaga tcgagattgg cgaggttgtt ctggcgcgcg
     1861 ccgcgaatgc ggatcgcgcg ccgcgtgtca gcagacacgg gcttggagtc gtcggccatg
     1921 agtgggatgg gcgcgggcag gggagcgccg ttgttgattg atgttactcg ggcctcgcgg
     1981 tgtgccggac gcgccttggg tcggtccgca cgggttgaac ggacgaaggg atgaatgcta
     2041 gctcgatact gtacataaat acagtatatc ggtgcgacga ccgcgcgcga ccgcaggccg
     2101 ctgctatgag cagccgcaat gctgaacaga tgtgggttgg cccggtctac gtcccggcaa
     2161 gcgtgggttc gtctatccga gcgacagagg ggcgggcggt tgccgcgagc gcgcagtgcg
     2221 cggcggtcca ccgtatacga gcgcccgttt cacttgcggg cccccgtcgg ccttgacccg
     2281 cgctatggag tcgatgcgaa gcggacgaag tcgtcgaact ggcgcgtgat caggcccgga
     2341 gtcggcccgc gatcgacctc gtcgcggcgg tcgaacacca tgtcggtgcc gacctgcagc
     2401 cgcacccagc gcggatacgc gtacatgtag ccggtcaggt ggtcgaccac catcaggctg
     2461 gatccgccgg gcagcagcat ctgcaacagc tgcatgttga cggcccgcga aaccacgacc
     2521 gcgcgcgaca gcgggtagcc gtcacgacgg cattcgacga tcagcgccga cgacgagcgg
     2581 cgcacatgca tgtcgaacat cggcgtgact ccgacgtact tcgccgatgt gttgctggcg
     2641 cggcaactca tgccatcgat cgccttgcct tcggcgtcga cggtctggat gttgaccttc
     2701 tgagtcggct cgccgctgag cgtcgcgcag cccgacagca ggccgagcac cagcgcggcg
     2761 agcagtggac ggcaaagaag acggatcatc ggtaagcgcc tggcgatcgg gacgggtacg
     2821 gcggctgcag gggacagtgc gggccagcgt accgggggag caaggacgac accctccggc
     2881 gaacggatta gctgggggcc ggctccagca ccct
//
LOCUS       DATIRT010000258         1661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_136512, whole genome shotgun sequence.
ACCESSION   DATIRT010000258 DATIRT010000000
VERSION     DATIRT010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136512"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="VLN25_05955"
     CDS             complement(<1..1136)
                     /locus_tag="VLN25_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HSQ10142.1"
                     /translation="MTTHYERRGDVAVLQIDNPPVNGLGHVTRKALLDGLERALDDAA
                     VKVIVVTGTGSVFSGGADIREFNTPAALAEPSLLQLIDAVESSPKSVIAAINGVCMGG
                     GLELALGCHYRIATPDASLGLPEVKIGLLPGAGGTQRLPRAVGADKALRMIVSGDPIP
                     AADALKCGLIERVVTRDSFDGVMQFAVEASRRSGRPRLRDRSASLPAGTDAGAYFAKA
                     RAEVGKQARGLPAPLKCVDAVEVSVALPFEEGMARERGYFLELVQSPESKALRHAFFA
                     ERAAAKIPDVPESTPTRPIRSAAVLGFGTMGGGIAMSFANAGIPVSVFEKDQAALDRG
                     LAMCRRNWEATAKKGRMPAEEVDKRLGLLRPTLDFTSLGSADIV"
     gene            complement(1169..>1661)
                     /locus_tag="VLN25_05960"
     CDS             complement(1169..>1661)
                     /locus_tag="VLN25_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSQ10143.1"
                     /translation="KTFEIAHLGIGYVPESRDVFPTLTVEENLKLGVKRGTAATWSYV
                     DVYRLFPRLQDRARTQAGVLSGGEQQMLTLARTMMGGPDFVIIDEPTEGLAPMIVELV
                     GKFLLELRDRGVSILLIEQKLAIAMQISQRVYVMGHGHMVFHGTPDELKANKLIRQEW
                     LEV"
BASE COUNT          281 a          585 c          509 g          286 t
ORIGIN      
        1 atgacgatgt cggcactgcc gagcgaggtg aagtccagcg tgggccgcag cagcccgagg
       61 cgcttgtcca cctcctccgc cggcatgcgg cccttcttcg cggtcgcctc ccagttgcgg
      121 cggcacatgg ccaggccgcg atcgagcgcc gcctggtcct tctcgaagac cgaaactggt
      181 attccggcat tcgcaaaact catcgcgatg ccgcccccca tcgtgccgaa tcccaggacg
      241 gcggcggatc ggatcggccg cgtcggcgtg ctctccggta catccgggat cttcgccgcc
      301 gctcgctccg cgaaaaacgc gtgccgcagc gccttcgact ccggcgactg caccagttcg
      361 aggaagtacc cgcgttcgcg cgccattccc tcctcgaacg gaagtgcaac cgacacttcg
      421 accgcatcca cgcacttcag cggcgccggc agtccgcgcg cctgtttgcc gacttcggcg
      481 cgggccttgg cgaaatacgc ccccgcgtcc gtgccggcag gcaacgacgc gctgcggtca
      541 cgcaagcggg gacggccact gcggcgggac gcttcgacgg cgaactgcat gacgccgtcg
      601 aaggagtcgc gcgtgaccac gcgctcgatc agtccgcact tcagcgcgtc agccgccggg
      661 attggatcgc cactgacgat catccgcagc gccttgtctg ccccgaccgc gcggggcagc
      721 cgttgcgtgc ccccggcgcc gggcaacagg ccaatcttca cttcgggcaa gccgagtgag
      781 gcatcgggag tcgcgatgcg gtaatgacaa cccagcgcca gttccaggcc tcctcccatg
      841 cacacgccgt tgatggcggc gatcacggac ttgggcgaac tctcgacggc atcgatcagc
      901 tgcagcaggc ttggctctgc cagtgctgcc ggcgtattga actcgcgtat gtccgcgccg
      961 ccggaaaaca ccgatccggt accggtcacg acgatgacct tgactgccgc gtcatccagc
     1021 gcgcgctcca ggccgtcgag cagcgccttg cgggtcacgt gaccgaggcc gttcaccggc
     1081 ggattgtcga tctgcagtac cgcgacgtca ccgcggcgtt cgtagtgggt ggtcattgcg
     1141 aaagactccg ggggacgacg cagggggctc agacttcgag ccactcctgg cgaatcagtt
     1201 tgttggcctt cagttcatcg ggcgtgccat gaaacaccat gtggccgtgc cccataacgt
     1261 acacccgctg cgagatctgc atggcgatcg ccagcttctg ctcgatcagc aggatggaca
     1321 cgccccgatc ccgcagctcg agcaggaact tgcccaccag ttcgacgatc atcggcgcga
     1381 gtccctcggt cggctcgtcg atgatcacga aatccggacc gcccatcatg gtacgcgcca
     1441 gcgtcagcat ctgttgttcg cctccggaca gcacgccggc ctgcgttcgc gctcgatcct
     1501 gcagacgcgg gaaaagccga tagacatcca cgtaactcca cgtcgccgcc gtgcccctct
     1561 tgacgccgag cttcaggttt tcctcgactg tgagcgtcgg gaaaacgtcg cggctttccg
     1621 gcacatagcc gatcccgaga tgggcgatct cgaatgtctt c
//
LOCUS       DATIRT010000259         3026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1612111, whole genome shotgun sequence.
ACCESSION   DATIRT010000259 DATIRT010000000
VERSION     DATIRT010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612111"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VLN25_05965"
     CDS             <1..266
                     /locus_tag="VLN25_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10144.1"
                     /translation="RRAAIVPQPSASGVGNTQPLSAMKALALLDTLQSADIVLPTTDG
                     REIRLRRITEPTADHKSLLRQLGLALPERFELNHKCSVDSATA"
     gene            complement(397..891)
                     /locus_tag="VLN25_05970"
     CDS             complement(397..891)
                     /locus_tag="VLN25_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10145.1"
                     /translation="MEYDGETGTFRYSLDEEKRVRAEKLDGSYLLKTDREDLTADEAW
                     RTYTLLTQAEAAFRTLKSPLRERPIFHHKERRVEAHVFLCLLAYHLLLSIEKTLLNGG
                     VHTSWATVREALRTHQVNTVVLPTDAGELRIRQGSTPERSHREIYTKLGIEAEIIPTR
                     KTWS"
     gene            complement(875..2239)
                     /locus_tag="VLN25_05975"
     CDS             complement(875..2239)
                     /locus_tag="VLN25_05975"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HSQ10146.1"
                     /translation="MYIRKASRTYKGKTYFSYLLVESVLTPKGPRQKVICSLGDLSPR
                     SRGEWLNLAHKLESALSGQPELLATGSDAEVDQLVARVHATQALTSSFVSPAASGRVS
                     ELVAVQVDRVRTEECREAGPVHVGYQFWVRLGVDTILTNAGLSQRARQVTCAMVLNRL
                     IHPDSELSMPAWIRSTALPDILGTDLVNLSEDALYRNLDRLHTRREPIEAALAERERS
                     LFNLDQTVFLYDVTSTFFEGKALANPKARRGYSRDHRSDCKQVLIGLAVNRDGFPLAH
                     EVFAGNRHDSTTLEEMLKALDRRIGLQAGQTVVVDRGMSGEENLKKIRQRQLHYLVAE
                     AYGARREWVEEFEDEQEFEQVKREVSPTNPFQHKSTIQVKRRKQGAETHVLCVSSERK
                     DKDRAIREAHEKKLVADLEKLQRRVSKGKGRGTKPAEVEQSIGRAERALLAGGAILPH
                     GVRR"
     gene            2495..>3026
                     /locus_tag="VLN25_05980"
     CDS             2495..>3026
                     /locus_tag="VLN25_05980"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HSQ10147.1"
                     /translation="MPRLRFHSKGILGLALVLALAPQLFGQATARIAGTVRDSSGAAV
                     PGAKITATNVNTGFELTRESLSDGTYSLPLLPVGEYRLSAQVQGFQPFVRSGLTLTVE
                     AIVTLDISLQVGQITEAVEVTAAAPLLEAQSGTLRGVIDQQRIVNLPLNGRDITQLVA
                     IQAGVVLRSTSSGEGNA"
BASE COUNT          562 a          982 c          852 g          630 t
ORIGIN      
        1 agcgacgcgc tgcaatcgtg ccacagccgt cggcgagcgg agtcggcaac acgcagccgt
       61 tgtcggcgat gaaggctctc gctctgctcg acacactgca gagcgccgac atcgttctac
      121 ccaccaccga cggacgcgag atccgcctcc gccgtattac cgagcccacc gccgatcaca
      181 agtcacttct tcggcagctc ggcctcgccc tcccggaacg tttcgaactc aaccacaaat
      241 gtagtgtaga ctcggcgacc gcctgaacac attttcaaca acttagccgt ctttcaccct
      301 cagtcgtgac gaacttcggc tagcccaact tccgcggttt tgaggtggga acgttgcaag
      361 ataaggactt agcgaatatt tagtcactat cgtggtctac gaccatgtct tgcgggtggg
      421 gatgatttcg gcctcgatac ccagcttggt gtagatctcg cgatgcgaac gctccggcgt
      481 cgagccttgt cggatgcgca gttctccagc gtcggtgggc agcacgaccg tgttcacctg
      541 gtgggttcga agtgcctcgc gcaccgtggc ccaggaagtg tggacgccac cgttgagcaa
      601 ggtcttctca atcgacaaca gcagatgata cgccagcagg caaagaaaca cgtgcgcctc
      661 cacccggcgt tccttgtggt ggaagatcgg ccgctcacgc aatggcgact tcagcgttcg
      721 aaaggcagcc tccgcctgcg tcagcagagt gtacgtccgc catgcctcat cggcggtcag
      781 atcctcgcgg tcggtcttca gcagatagct tccatcgagt ttctccgctc gcacgcgctt
      841 ctcttcgtca aggctgtagc ggaacgttcc ggtctcaccg tcgtactcca tgcggtaata
      901 tcgcgccacc cgcgagtagc gctctttcag cccggccgat ggattgctcc acttccgccg
      961 gtttcgtccc ccgccctttc cccttgctca cgcgcctttg caacttctcc agatccgcca
     1021 ccagcttctt ttcgtgcgcc tcgcggatgg cccggtcttt gtccttgcgt tcgctgctca
     1081 cgcacaacac gtgcgtctcg gctccctgtt tgcgccgctt cacctgaatc gtcgacttgt
     1141 gttgaaacgg attggtgggt gagacctccc gcttcacttg ctcgaactcc tgctcgtcct
     1201 cgaactcttc cacccactcg cgtcgcgctc cgtacgcctc ggccaccagg tagtgcaact
     1261 gccgttgcct gatcttcttc aggttctctt ctcccgacat gccccggtcc accacgacgg
     1321 tctgtccggc ctgcagaccg atccgccgat ccagagcttt cagcatttcc tccagcgtcg
     1381 tggaatcgtg acggtttccc gcaaacacct catgcgccag tggaaacccg tcccgattca
     1441 ccgccagccc aatgagaacc tgcttgcagt ccgaacggtg atcgcgcgag tagccacgcc
     1501 tggccttcgg gttggccagc gccttgccct cgaagaaagt ggacgtcaca tcgtacagga
     1561 atactgtttg atccaggttg aacaggctgc gctcgcgttc tgccagggcc gcttcgatcg
     1621 gctcccgcct ggtatgcagg cggtcgagat tgcgatacag tgcgtcctcg ctgaggttca
     1681 ccaagtccgt gcccagaatg tcgggcagcg ccgtggaacg aatccaggcc ggcatgctca
     1741 gttcgctgtc cggatggatc aaccggttca acaccatcgc acaggtcacc tgccgggcgc
     1801 gctggctcag ccccgcattc gtcagaatcg tgtccacgcc gaggcgcacc cagaactgat
     1861 agccgacatg tacggggcca gcctcgcggc attcctcggt gcgtacccga tcgacctgta
     1921 ccgccaccaa ctcggagacc ctgccggacg cggccggcga gacaaatgag cttgtgagcg
     1981 cctgcgtggc gtgcaccctg gctacgagct gatccacttc ggcatcggaa ccggtcgcga
     2041 gcaactccgg ttgaccggat aaagccgact cgagtttgtg agccaggttg agccactcgc
     2101 cgcgcgaacg cgggctcagg tctccgagtg agcagatgac cttctgccgt ggacctttgg
     2161 gagtcagcac gctttcgacc agcagatagc tgaagtaggt cttgcccttg taagtccgcg
     2221 aggctttgcg gatgtacatg tctgtagaca ctatggagca ggacggcacc gattgcaata
     2281 gtaaacagct cagaacaacc gggaattccg gtcgcggaca gcgctcgttt tagtcactat
     2341 tttcgccgaa gtaagaccgg cgtattgaaa tcactggact tgcggtcgcc aaaaccggaa
     2401 aaactaagca ccacctcgct aggtggcgcg gaagttgggc taagtctggt tcagtagtct
     2461 gtccgtgaca tgatgtccct aagggggatt ttcattgcca cgactgcgtt ttcacagtaa
     2521 aggaatcctt ggcctcgcgc tcgtgctggc gcttgcgcct cagctcttcg ggcaggcgac
     2581 tgcgaggatt gcgggcaccg tgcgtgacag ctccggcgct gccgttccag gcgctaaaat
     2641 cacggccaca aacgtaaaca ccggcttcga gctgacgcgc gagtcgctct cggacggtac
     2701 gtattcactg ccgctgctgc cggtgggcga gtaccgcctt tcggcacagg tacaaggctt
     2761 tcagcccttc gtgagatccg gtctgacgct caccgtcgag gccatcgtga cactcgacat
     2821 ctcgctgcag gtcggacaga tcacggaggc cgtagaagtc acggcagccg ccccactgct
     2881 cgaagcgcag agcggaacct tgcgaggcgt gatcgatcag cagcgcatcg tcaacctgcc
     2941 tctgaacggg cgcgacatca cgcaactggt cgccattcag gccggcgtcg tcctgcggag
     3001 tacatcgtcc ggcgagggca acgctt
//
LOCUS       DATIRT010000260         6283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_432648, whole genome shotgun sequence.
ACCESSION   DATIRT010000260 DATIRT010000000
VERSION     DATIRT010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6283
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432648"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..264)
                     /locus_tag="VLN25_05985"
     CDS             complement(136..264)
                     /locus_tag="VLN25_05985"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSQ10148.1"
                     /translation="MFKKILLPTDGSDLSTKAIDGGLEFARALKAGVVGVTVIEPY"
     gene            complement(325..1131)
                     /locus_tag="VLN25_05990"
     CDS             complement(325..1131)
                     /locus_tag="VLN25_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HSQ10149.1"
                     /translation="MNTVDLSPPEADLARRFGGVARLYGEEGAAKLRRAHVAVIGIGG
                     VGTWAAEALARSAVGVITLIDMDHIAESNTNRQIHALGNDYGRAKVEAMATRIAAINP
                     SCDIRPIDDFITVDNAARFIAGYDFVLDCIDQVNAKAALIARARAVNIPVVTCGAAGG
                     RLDPVRISRGDLAVIAGDPLLAKVRQRLRQDYGFPRESGARRTQFHVLAVYSDELVQR
                     PAPASGEGEDVVGGLACAGYGSSVAVTATMGFVAASVALTQIAQAPDGGA"
     gene            complement(1142..2083)
                     /locus_tag="VLN25_05995"
     CDS             complement(1142..2083)
                     /locus_tag="VLN25_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF533 domain-containing protein"
                     /protein_id="HSQ10150.1"
                     /translation="MDAKEQQAVMTIALLAAFADGRNADREREELRRVADALGTSLNT
                     PALVQDVLLKRTSMDAAAAALTTPESRRLAFELAVGVCDVDGLRNEAETRFLAELGNR
                     LGLDQPTIVETAVDADELATAPLPAAGAASNDVVAAAPSMPAAELDRMILNASILNGA
                     LELLPQSIASMAIIPLQMKLVYRIGQSHGYEADKTQIRDLLATLGAGLTGQYLEQIGR
                     KLVGGLFGKVAGGMAGSLARGATGAMFSFATTYAIGEVARRYYAGGRVMSADLLKRTF
                     SELLSQGKAMQAQYLPQIEQQARTLDVSRIVQMVRNN"
     gene            2263..3162
                     /locus_tag="VLN25_06000"
     CDS             2263..3162
                     /locus_tag="VLN25_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSQ10151.1"
                     /translation="MKILVPVDGSEFSRAAIEFVASRSTLIGTEPQVDVINVQLAVPA
                     RAARVVGKAALTEYYTDESDKALKPALRRLAKAGLTATAHYLVGHPGEEISAAALRDK
                     VDLIVMGSHGHSALRGLLFGSVTNNVLARTRVPMLVVRHRETPTTDTLKVGIAVDGSK
                     YGREAVKYALRHLALFGARPEVTLLHVVSDFAGAVMPDMGGIALPAFSEDEIRMLQKK
                     AFEAAVGPARKLLAKGNIKPTEVCLVGNAGDELCAYAKKKKLDVLVMGSHGYGAFKAA
                     VLGSVATRVMAHSNVPLLLVRHA"
     gene            3491..>6283
                     /locus_tag="VLN25_06005"
     CDS             3491..>6283
                     /locus_tag="VLN25_06005"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSQ10152.1"
                     /translation="MLMMFSSAADAMKFAMAYHRALAGLPVPLTARAGLHVGPVLLRE
                     NSADDIALGAKPLEVEGMAKPTAARVMSLARGGQTLLTPEARENLGPTELKVESLGHW
                     MVKGVEEPIELFEAGAPGTRFIPPTDSEKVYRVVRAADWWLPVKDIPNNLPFQATSFI
                     GRETELDEVKSYLHRVRLLTLLGMGGLGKTRLCLQVAAETMHLYPDGVWFLDLAPLRD
                     DAHVAAEAAQAIGVKAEPDRTLLQAICAHLKSRRVLIILDNCEHLVKASAELAQAILR
                     AAPYVRLLATSREALHVPGEQSYPVHPLPLPPRGAGIDDVSQSTAVRLFVERAQQHKP
                     SFTLDATQAAAVAELVGRLEGIPLALELAAARVKSLPVAEINARLKDRYKILTGGARV
                     LQERQQTLRALVDWSYDLLTQKEQTLFARLGIFVGGFDLAAAEQICGTDPLACDDILD
                     LLASLVEKSLIMLDESDEGGRYRMLETIRDYAVERLEQHEDSTSVATRHCDHYFAMSK
                     AARDGMQGPEQAEWIRRVETDIDNMRSAMSLALDGGVDQFIAVKMAVALQPFWRLRGY
                     ATEGRKLLRMALAKPEIAGSDLAQAWALYVAAALAESQSDHREAREMLENCLELRRRL
                     GNPVDLAATLSTLSLVKLQTGDVAGAAECEKEALRIFRDIGHRRGELIGLIHLGQVAE
                     HLGQDQDAKSSFEEAFVIAQEIKHQALQGECELLLGQLALAEGATTDAELWFKRSLTL
                     CREAADRRGEANALRWLGKCDIGAGALPSARSRLRDALKAFRNLEMWDELLGCLEDLA
                     SLWQAEGRPSLSIQISAATQRAREKLGLRRAPREEARQNELLASYRASATGGSAEDAW
                     IEGSTWKVEDAVRCALDFEADRDEVPQVEPRESDGHCPRLKRYCAIGFEVEGATNGVL
                     DLPGRTLDPRVFRATTC"
BASE COUNT         1133 a         2029 c         2047 g         1074 t
ORIGIN      
        1 agtgaccgtt tcgaccggga cggacgcttt ggccgcggca tcggccacct tgccgagccg
       61 ctcggcggcg atcttcagcg tgcgctgctc gtagtcgtcg agcgtctccg gacggtactc
      121 ggacagattc gagtactaat aaggctcgat caccgtcacg ccgaccacac cagccttcag
      181 cgccctggcg aactccagtc caccgtcaat cgccttcgtc gacagatcgg agccgtcagt
      241 cggtaacagg attttcttga acattgattg ctaactcctt caagtaaagc ctaggccacc
      301 gcccatcgtc accgttgaca ggcgtcaagc gccgccgtct ggcgcctgcg cgatctgcgt
      361 caatgcaacg gacgccgcga caaaacccat ggttgccgtg acagccacgc tggaaccgta
      421 gcccgcacac gcgaggccac ccaccacgtc ctcaccctcg ccagaggctg gggccggccg
      481 ctgcaccagt tcgtcggagt agacggccag tacgtgaaac tgtgtccggc gcgcgccgga
      541 ctcacgcggg aaaccatagt cttgacgcag cctttgccgg accttcgcca gcagcggatc
      601 gccagcgatg acggcaaggt cgccgcgcga aattctcacc ggatccagcc gaccccccgc
      661 ggcgccgcag gtgacgaccg ggatgttgac ggcgcgcgcc cgggcgatca gcgcggcctt
      721 tgcattcact tggtcgatac agtcgaggac gaagtcgtag ccagcaatga accgcgccgc
      781 attgtcgacg gtgatgaagt cgtcgatcgg acgaatgtcg caggacgggt tgatggccgc
      841 gatgcgcgtc gccatggctt cgaccttggc cctcccgtag tcattgccca gggcgtggat
      901 ctggcggttc gtgttcgact cggcgatgtg atccatgtcg atcagggtga tcacgccgac
      961 cgcggagcga gcgagcgcct cggccgccca ggtgccgacc ccgccgatgc cgatgacagc
     1021 gacatgggcc cgccgcagct tcgcggcgcc ctcttcgcca tagagccgcg cgacgccacc
     1081 gaatcgacgc gcaaggtcgg cctcgggtgg cgacagatcg acggtgttca agcggccggg
     1141 cttaattgtt ccgcaccatc tgcacgatgc gcgaaacatc caacgtgcgt gcctgctgct
     1201 cgatctgcgg caggtactgc gcctgcatcg ccttgccttg cgatagcaac tcgctgaacg
     1261 tgcgcttgag cagatcggcc gacatgacgc gaccgcctgc gtaatagcgg cgcgccacct
     1321 cgccgatggc gtacgtcgtc gcgaacgaga acattgcacc ggtcgcaccg cgcgccagcg
     1381 agccggccat gccgccggcc accttgccga acagtccccc gaccagcttg cggccgatct
     1441 gctccagata ctgcccggtc aggcccgcgc cgagcgtcgc gagcaggtcg cggatctggg
     1501 tcttgtccgc ctcgtaacca tgtgactggc cgatgcgata gacgagtttc atctgcagcg
     1561 gaatgatcgc catcgaggca atcgactgcg gcagcagttc gagcgcgccg ttcagtatcg
     1621 acgcattgag aatcatccgg tcgagttctg cggcgggcat cgacggagcc gcggcgacca
     1681 catcatttga cgctgcgccg gcggccggca acggcgcggt tgccaactcg tcggcatcga
     1741 ccgcggtttc gacgatggtc ggctggtcca gtcccagccg attgccaagc tcggcgagga
     1801 agcgtgtctc cgcctcgttg cgcagcccgt cgacgtcgca gactcccacc gcgagttcga
     1861 atgccagccg gcgcgattcc ggcgtggtga gggctgccgc ggccgcgtcc atggaggtcc
     1921 gcttcagcag cacatcctgc accagcgccg gcgtgttgag cgaggtgccc aatgcatcgg
     1981 ccacgcgtcg cagctcctcg cgctcgcgat ccgcgttgcg gccgtcggca aacgccgcga
     2041 gcagggcgat ggtcatgacg gcttgctgtt ctttggcgtc catgcggctt ctccactgat
     2101 gcgagcttac gcgtcccgcg cagcgctcga tccgacccat cttcggtcgg ccggcgcgat
     2161 ttcaatacgg agttccccgg aatttgccag ccgtcacgcg gtaccgcagg ccctgccgta
     2221 gactgatatc gatcggtcaa agatggttcg acggagacat caatgaaaat tctcgtcccg
     2281 gtagatggca gcgagttcag ccgtgccgca atcgaattcg tagcttcgcg gtccacgctg
     2341 atcggcaccg agccgcaggt cgacgtgatc aatgtccagc tcgccgtgcc ggcgcgcgcc
     2401 gcacgcgtcg tcggcaaagc ggcgctcacc gagtactaca cggacgaatc cgacaaggcc
     2461 ctgaagccgg cgttacggcg gctggccaag gcgggactga ctgctaccgc acactatctc
     2521 gtgggccatc cgggcgagga gatcagtgct gctgcgctcc gcgacaaggt cgacctcatc
     2581 gtaatgggct cgcatggcca ttcggcactg aggggcctgc tgttcggttc ggtcaccaac
     2641 aatgtgctcg cgcgcacgcg cgtgccgatg ctggtcgtgc gacaccgcga gacgccgacc
     2701 accgacacgc tgaaggtggg gatcgcagtc gacggcagca agtacggtcg cgaggcggtc
     2761 aagtatgcat tgcgtcatct cgcattgttt ggcgccaggc cggaggtcac gttgctccat
     2821 gtcgtcagtg atttcgccgg cgcggtcatg cccgacatgg gtgggatcgc gttgccggcg
     2881 ttttcggaag acgagatccg catgctgcag aagaaggcct tcgaggccgc cgtcggcccc
     2941 gcccgcaagc tgctggccaa aggcaacatc aagccgaccg aggtctgcct cgtaggaaac
     3001 gccggcgacg aactgtgcgc gtacgcgaag aagaagaaac tcgacgtact ggtcatgggc
     3061 tcgcacggat acggtgcctt caaggccgcc gtactggggt cggttgcaac ccgcgtgatg
     3121 gcacactcca acgtgccgct gctgctggtc cggcacgcct gaagcggcgc tgctggcggc
     3181 gtgcgcattg ccgccggagc agtccagcag accgtacact gtcgcgcgcc gtaggccccg
     3241 ggatcgtcct ggcgacccga gggcgcgatc gttgtcaatc tccaaggcgg attgatcggc
     3301 gggcgaacgt gcccgccacc ccatgatgag tgaaattcga gcgctgctcc tgaccgacgt
     3361 cgtcgacagc acgaagctcg ccgaatcgat cggcgatgcg gcgatggcct cggtatgagc
     3421 ggcgcacgac cgggtcgccc gcgatctgct gcaggttcac catggtaggg agatcgacaa
     3481 gaccgacggc atgctgatga tgttcagcag cgctgcggac gcgatgaaat ttgcgatggc
     3541 ctatcaccgt gcgctcgccg gcctgccggt gccgctgacc gcgcgcgccg gcctgcatgt
     3601 cggccccgtg ttgcttcgcg agaacagtgc cgatgacatc gccctcggcg cgaagcccct
     3661 ggaggtcgaa ggcatggcca agcccaccgc cgcgcgcgtg atgtcgctcg cccgcggcgg
     3721 ccagacgctg ttgacgccgg aggcgcgcga gaacctcggc ccgaccgaac tcaaggtcga
     3781 gtcccttggc cactggatgg tcaagggggt cgaggagccg attgaactgt tcgaggccgg
     3841 tgccccggga acgcgcttca tcccacccac ggacagcgaa aaggtctatc gcgtcgtgcg
     3901 cgcggccgac tggtggctgc cggtcaagga cattccgaac aatctgccgt tccaggccac
     3961 gtcgttcatc ggccgcgaga ccgaactcga cgaggtcaaa tcgtatctgc acagagttcg
     4021 cctgctgacg ctgctcggca tgggcggcct ggggaagacg cggctctgcc tgcaggtcgc
     4081 cgcggaaacg atgcacttgt atccggacgg cgtctggttc ctcgacctgg caccactgcg
     4141 cgacgacgcc cacgtggccg ccgaggccgc acaggcgatc ggcgtgaaag ccgagccgga
     4201 tcgcacactg ttgcaggcga tctgcgccca tttgaagtct cgccgcgtac tgatcatcct
     4261 cgacaattgc gagcacctgg taaaggcctc ggccgagctg gcccaggcga tcctgcgcgc
     4321 cgcgccgtac gttcgacttt tagcaacgag ccgagaggcg ctgcacgtgc ccggcgaaca
     4381 gagctacccg gtgcatcccc tgccgctgcc gccgcgtggc gccggcatcg atgacgtgtc
     4441 ccagtcgacc gcggtgcgcc tcttcgtgga acgcgcgcag cagcacaagc cctcgttcac
     4501 gctcgacgcg acccaggccg cggcggtggc cgaactggta ggaaggctcg aaggcattcc
     4561 cctcgcgctc gaactcgcgg ccgcgcgagt caagtcgttg ccggtggcgg agatcaacgc
     4621 gcgcctgaag gaccgctaca agatcctgac cggcggcgca cgcgtgctgc aggaacggca
     4681 gcaaaccctg cgagcactgg ttgactggtc ctacgacctt ctgacgcaga aggaacagac
     4741 gctgttcgca cggctcggca tctttgtcgg cggcttcgac ctcgccgccg cggagcagat
     4801 ttgcggaacg gacccactcg cttgcgacga catcctggat ctgctggcgt cgctggtcga
     4861 gaagtcgctg atcatgctcg acgaaagcga tgagggcggg cggtacagaa tgcttgaaac
     4921 gattcgagac tatgcggtcg agaggcttga gcagcacgag gactcaacct cggtagcgac
     4981 gaggcactgc gatcattatt tcgccatgtc aaaggcggcg cgcgatggca tgcaaggccc
     5041 cgagcaagcc gaatggatac ggcgggtcga gaccgacatc gacaatatgc gcagcgcgat
     5101 gtccctggcg ctggatggcg gtgtcgacca gttcatcgct gtgaaaatgg ccgtggcatt
     5161 gcagcccttc tggagacttc gcggctatgc gaccgagggc cgaaagctgc tgcgtatggc
     5221 cctcgccaag cccgagatcg cgggctctga tcttgcccag gcgtgggcgc tgtacgtagc
     5281 tgccgctttg gccgagagtc agagcgacca ccgggaggcg cgagagatgc tcgagaactg
     5341 ccttgaactc aggcgccggc tcggcaatcc cgtcgatctt gctgcaacgc tgtcgacgct
     5401 ttcgctggtg aaattgcaga ctggcgatgt cgcgggtgcc gccgagtgcg aaaaggaggc
     5461 gctgcggatc tttcgagaca tcggccatcg tcgcggagaa ctgatcgggc tgatccatct
     5521 cggacaggtc gccgagcatc ttggccagga ccaggatgcg aaatcgagct tcgaggaggc
     5581 gtttgtcatt gctcaagaga tcaaacacca ggcattgcag ggagagtgcg aactgctact
     5641 cggtcaactc gcgctcgcag agggtgcgac gacggacgcc gagctgtggt tcaagcgctc
     5701 ccttaccctc tgccgcgagg ccgcggatcg gcgcggcgaa gccaatgcgc tgaggtggct
     5761 aggaaagtgc gacatcggcg ctggcgccct gccttcagcg cggagccggc ttagagatgc
     5821 attgaaagcg tttcgaaatc tcgagatgtg ggatgaattg ctcggctgcc tcgaggacct
     5881 agcctcgcta tggcaggccg aaggtcgtcc cagtctatcg atccagattt ccgcggcgac
     5941 ccaacgtgcg cgagaaaagc tgggattgcg ccgggcgcca cgggaagaag cgcggcagaa
     6001 cgagttgctc gcgagctatc gggcctcggc aacaggtggt agcgcggaag acgcgtggat
     6061 cgagggttcg acctggaagg tcgaggacgc cgttcgttgc gccctcgact tcgaagccga
     6121 tcgcgatgag gtcccgcaag tcgaaccgcg tgagtcggac ggccactgtc cgcggttaaa
     6181 gcgctattgc gcgatcggct tcgaagtcga gggcgcaacg aacggcgtcc tcgaccttcc
     6241 aggtcgaacc ctcgatccac gcgtcttccg cgctaccacc tgt
//
LOCUS       DATIRT010000261         1619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_136854, whole genome shotgun sequence.
ACCESSION   DATIRT010000261 DATIRT010000000
VERSION     DATIRT010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136854"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VLN25_06010"
     CDS             complement(<1..354)
                     /locus_tag="VLN25_06010"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSQ10153.1"
                     /translation="MVGESGSGKSVSAMSIVRLLPENAIVAPESRVEFGGRNLLADPI
                     ESLRAIRGKDISVVFQEPMSSLNPVFPVGWQIAEVLQLHMGLSRKQVGGRVLELMTEV
                     GIPEPKSRIDSYPHEL"
     gene            complement(474..1526)
                     /locus_tag="VLN25_06015"
     CDS             complement(474..1526)
                     /locus_tag="VLN25_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSQ10154.1"
                     /translation="MTTVVEAYQAPSTERSAGVWVTAWRRLKGDRVGMISLAVVAGFL
                     VMILASVLGIVAGDWQKEVGVPDAPPTFVGPRAASTGTDPIASVIAKDAKPVDLSDID
                     PLAPKYKEWAERAAKIQITEAPRADTLPIGADRLGRDVLSKVIKGAEVSIFVGVFAAV
                     LATFIGTILGAVSGYAGGKIGDFLEWVYNVFTSIPGILLIFAFAAVLGRGISTVILIL
                     GLAGWTGIYRLVRAEYMKHRTREYVRAAEAIGASHFARMFVHILPNVSHVVLVQLSLH
                     AVFFIKAEVILSFLGLGVRVDQVSWGTMLSEAQAELVLGHWWQLVATTISMAFFVTAF
                     SLLTDALRDALDPKLR"
BASE COUNT          328 a          542 c          494 g          255 t
ORIGIN      
        1 aagctcatgc ggatacgagt cgatgcgcga ctttggctca gggatgccga cctcggtcat
       61 cagttccagg acccggccac cgacctgctt gcgcgacagc cccatgtgga gctgcagcac
      121 ctcggcaatc tgccagccga ctggaaagac cggattcagc gacgacatcg gttcctggaa
      181 cacgaccgag atgtccttgc cgcgtatcgc gcgcagggac tcgatcgggt cggcaagcag
      241 gttgcgacca ccgaactcga cccggctttc cggcgcgacg atcgcatttt cgggcagcag
      301 ccgcacgatc gacattgccg acacgctctt gcccgacccg gattcgccaa ccagcgcaac
      361 ggtggcgttc tacggaatgt cgaagctgat gcccctcaca gcctcggcga cacggtgctt
      421 gccgagccgg aacgaaacgc gcaggtcgcg caccgaaatg agattggaca tgtctatctc
      481 aattttggat cgagcgcatc gcgcaacgca tcggtaagca aggagaacgc ggtgacgaag
      541 aacgccatcg agatggtggt ggccaccagc tgccaccagt gccccagcac aagctcggcc
      601 tgcgcctcgg acagcatcgt tccccacgac acctggtcga cgcgcacgcc caggcccagg
      661 aacgaaagaa tgacctccgc cttgatgaag aacaccgcgt gcagcgacag ctgcaccagc
      721 acgacgtggc tgacgtttgg caggatgtgc acgaacatgc gcgcaaagtg gctggcgccg
      781 atggcttcgg ccgcccggac gtactcgcgc gtgcggtgct tcatgtactc ggcgcgcacc
      841 agccgataga tgccggtcca ccctgccaga ccgagaatca ggatcacggt gctgatgccg
      901 cggcccagca ccgcggcaaa cgcgaagatc agcaggatgc ccgggatcga cgtgaagacg
      961 ttgtagaccc actcgaggaa gtcgccgatc ttgccaccgg catacccgga gacagcgccc
     1021 aggatggtgc cgatgaatgt cgccagcacc gcagcgaaaa caccgacgaa gatcgagact
     1081 tccgcgccct tgatcacttt cgacaggaca tcgcgcccga gccggtcggc accgatcgga
     1141 agcgtgtcgg cgcgcggggc ctcggtgatc tggatcttcg cagcacgctc cgcccactcc
     1201 ttgtacttgg gcgccagcgg atcgatgtcc gacaggtcga cgggcttcgc gtccttcgcg
     1261 atcacgctgg cgatcggatc ggtgccagtg ctggcggcgc gcgggccgac gaaggtcggt
     1321 ggtgcatcag gtacgccgac ttccttctgc cagtcgccag cgacgattcc cagcaccgat
     1381 gccaggatca tcacgagaaa gcccgccacg acggccagcg agatcatgcc gacccggtct
     1441 cccttcaggc gccgccaggc ggtcacccag acgcccgcgg atcgctcggt ggacggcgcc
     1501 tgataggcct cgacaacagt cgtcatcgca tcctcacttc agcacgctca tttgagcacg
     1561 acccgcgggt cgaccaactt gtagaggatg tcggccccga ggttgatcac catcgtcag
//
LOCUS       DATIRT010000262         4243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_432992, whole genome shotgun sequence.
ACCESSION   DATIRT010000262 DATIRT010000000
VERSION     DATIRT010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432992"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="VLN25_06020"
     CDS             <1..988
                     /locus_tag="VLN25_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HSQ10155.1"
                     /translation="MLHSFDGGGVTAWGPALLVRKSLLNKASLFATYYADIVSNASID
                     VVTTASPYDETRHEYGVGFDYAVRDSLITLAGSYSTEPDYTATAFNIDVATDVFSGMT
                     TVGLGYTYGSDDVGKVGEGFFDTAKHWRYRVGVTQILTPRWYANANFEVVSDDGFLGS
                     PYRMALVFGAAVPERMPRTRTSRTAQFRLVGEVATNWAVRAEYRYFWDTWDIAAHTFG
                     LGASTRFGDKWLVDAYVRGYRQEGALFYSDNATSETLYVMRNRHLSNYNSWTIGTKAT
                     YALTRQPGRYEIFLNGAVEYWMTDYSDFTDLRTGSLYSYNATVLQLYVSALF"
     gene            1012..1602
                     /locus_tag="VLN25_06025"
     CDS             1012..1602
                     /locus_tag="VLN25_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HSQ10156.1"
                     /translation="MSNRSLLIAAFLALTVLMGASAQPTPAPASSNAPASTPAPAAAA
                     APTPTGSLDDRIQDAKSDVIRLNRDLMVLEEELLFPANTQVAIFVSMDVGKMFLLDSV
                     RVKLDGKDVAGYLYTPAEVQALHRGGVQRLYLGNLKSGTHELVAFFTGKGPHERDYKR
                     GATIRIEKGVDPKYIELQIKDSTGKLQPEFEVKVWQ"
     gene            1625..3628
                     /locus_tag="VLN25_06030"
     CDS             1625..3628
                     /locus_tag="VLN25_06030"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSQ10157.1"
                     /translation="MNCLTERWARAAALAAVCACLASAVHAQPAGATRPAGSSLPFSW
                     MDMSNWLNWWPWSDTTPPPPGGVESPHYGDSLFYFYQGRYFSSVTRLMASQQFGRMVP
                     HDDEAEILRGGLFLSYGMHREAGEVFARLIERGAPPPVRDRAWYYLAKIRYQRGLYAE
                     SEDALGRIDKPLAGALEEDRALLQANVLMARGKYTEAIRVLDVLAKGPGATSYVRYNL
                     GVALVRSGDLARGTKLLDEVGKLPATSDEFRSLRDKANVALGFASLQEGRSEPARAYL
                     ERVRLSGMYSNKALLGFGWAAAAQGQMKSALVPWSELATRDPGDAAVLEAKLAVPYAF
                     ADLGASAQALELYQDAIGVFDRESVNLDKSVAAIRSGKLLDGLMASNPGEEMGWFWSI
                     SELPEGQDLPLGGHLALLMATHPFQEAFKNYRDLLYLSRNLQQWQESLGVLRDMLANR
                     REAFAARLPQIQEKERALNLAGFEQRFESLQVELDRVERDGDAAAFATVRERELQARL
                     DRVRETLNLAAKDSGLTHAELAQANERARRAAGALLWQQNDQFAVRLWSAKKGMQELQ
                     RNLAQAQQRDAALVAAQRDEPARLDAFAGRLDLLAARVDTMVPRVAVLTQEQQAAVQE
                     LAVAELLQQKERLAAYGTQARFAVAQIVDRASLAKESARVPAQ"
     gene            3612..>4243
                     /locus_tag="VLN25_06035"
     CDS             3612..>4243
                     /locus_tag="VLN25_06035"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSQ10158.1"
                     /translation="MCPRSSLAVARSSAILAVALLAACSSPFKRAQPEPPTLKSLASH
                     QVTVNPDIGIKATEEDAARAYRDFLAASPREPQRQEALRRLGDLEMDLVDSRMATGET
                     TGSEADYKAAVTRYQDFLKTYPNDSGNDRVLYQLARAYELSGNLETSLATLDKLVLLY
                     PTTRYRDEAQFRRGELMFALREYPKAEAAYLVTMRGDKSSPYYERSIYMHG"
BASE COUNT          760 a         1377 c         1402 g          704 t
ORIGIN      
        1 gatgctgcac agcttcgacg gcggtggtgt gaccgcgtgg gggccggcgc tgctggtgcg
       61 caagagcctg ctgaacaagg cgtcgctctt tgcgacttac tacgcggaca tcgtcagcaa
      121 cgcgtcgatc gacgtggtga ctaccgccag tccgtatgac gaaacccggc acgagtacgg
      181 ggttggcttc gactatgcgg tgcgcgattc gctgatcacg ctggcggggt cgtacagcac
      241 cgagcccgac tacaccgcca ccgcgttcaa catcgatgtt gcgaccgatg tcttttcggg
      301 catgaccacg gttggcctcg gttacaccta cgggtcggac gacgttggca aggtcggcga
      361 agggttcttc gacaccgcga agcactggcg ctaccgggtc ggcgtgacgc agatcctcac
      421 gccacgctgg tacgccaacg cgaacttcga ggtcgtgtcg gacgatggct tcctcggcag
      481 cccttaccgc atggcgcttg tcttcggcgc cgcggtgccg gagcgcatgc cgcgcacccg
      541 caccagccgg accgcccagt tccgcctggt cggcgaggtc gcaaccaact gggcagtgcg
      601 cgccgagtat cgctacttct gggatacctg ggacatcgcc gcgcacacct tcggcctcgg
      661 cgcgtcgacc cgcttcggcg acaagtggct ggtcgacgcc tatgtgcgcg gctatcgcca
      721 ggagggcgcg ctgttctact ccgacaacgc taccagtgaa acgctgtacg tcatgcgcaa
      781 ccgccatttg agcaactaca actcgtggac gatcggcacg aaggcgacct acgcgctcac
      841 acgccagccc ggccggtacg agatcttcct gaacggggcg gtcgagtact ggatgaccga
      901 ttacagcgat ttcacggacc tgcggacggg cagtttgtac tcgtacaacg cgaccgtact
      961 gcagttgtac gtgtctgcgc ttttctgatt ttccgactaa gggaccgcag tatgtcgaac
     1021 cgcagcctgc tcatcgccgc tttcctggcc ctcacggtgc tgatgggtgc atcggcacag
     1081 ccgacgcccg cgcccgcatc gtccaacgcg ccggcttcca cacctgcgcc ggccgccgcc
     1141 gccgcaccga cgccgaccgg cagcctcgat gaccgcatcc aggatgccaa gtccgatgtg
     1201 atccggctca accgcgacct gatggtgctc gaggaagaat tgctgttccc ggccaataca
     1261 caggtcgcga tctttgtgtc gatggatgtg ggcaagatgt tcttgctcga ctcggtgcgg
     1321 gtgaagctcg atggcaagga cgtcgcgggc tacctctaca cgccggctga ggtccaggca
     1381 ctgcaccgtg gcggagtgca gcgactctat ctgggcaacc tgaagtcagg cacgcacgag
     1441 ctcgtggcct tcttcaccgg caagggaccg catgagcgtg actacaaacg cggcgcgacg
     1501 atcaggatcg aaaagggcgt cgatccgaaa tacatagaac tccagatcaa ggattctacc
     1561 ggcaagctgc agccggaatt cgaggtcaag gtctggcagt gaccacgcga tttctcaggc
     1621 aacgatgaac tgtctcactg aacgctgggc gcgcgccgcg gcgctggcgg cggtgtgcgc
     1681 gtgcctcgct tcggccgtac atgcacagcc ggctggcgcg acgcggccgg cggggtcgtc
     1741 gctgcccttc agctggatgg acatgtcgaa ctggctgaac tggtggccgt ggagcgatac
     1801 gactccacca ccgccgggcg gcgtcgagtc cccgcactac ggcgacagcc tgttctactt
     1861 ctaccaaggc cgctacttct cgtcggtcac ccggttgatg gcgtcgcagc agttcggccg
     1921 catggtgccg cacgacgatg aagccgagat cctgcgcggt ggtctgttcc tgtcctacgg
     1981 catgcaccgc gaggcgggcg aggtcttcgc caggctgatc gagcgcgggg ccccgccgcc
     2041 agtgcgcgac cgcgcctggt actacctcgc caagatccgc taccagcgcg gcctgtatgc
     2101 agagtccgag gacgcgctgg gccgcatcga caagcccctg gcgggcgcgc tggaagagga
     2161 ccgcgctctg ctgcaggcca acgtcctgat ggcgcgtggc aagtacaccg aggcaatcag
     2221 ggttctggat gtcctcgcca agggaccggg tgcgaccagt tacgttcgct acaacctcgg
     2281 cgtggcgctg gtacgaagcg gcgacctggc gcgcggcacg aagttgctcg acgaggtcgg
     2341 caagctgccg gccacgagcg acgagttccg cagtctgcgc gacaaggcga atgtcgcgct
     2401 cggcttcgcg tcgctgcagg aaggccgaag cgaaccggca cgcgcctacc tggaaagggt
     2461 gcgactgtcg ggcatgtact cgaacaaggc gctgcttggg ttcggctggg ctgccgccgc
     2521 ccagggtcag atgaaatcgg cactggtgcc gtggagcgaa cttgccacgc gcgatccggg
     2581 cgatgcggcc gtcctcgagg ccaagctggc cgtgccgtat gcattcgcgg atctgggcgc
     2641 cagcgcccag gcactcgagc tgtaccagga cgccatcggt gtattcgacc gcgagagcgt
     2701 caacttggac aagtccgtcg ccgcgatccg cagcggcaag ctgctcgacg gcttgatggc
     2761 cagcaatccg ggcgaggaga tgggctggtt ctggagcatc tccgagctgc ccgaggggca
     2821 ggacctgccg cttggcgggc atctcgcact gctgatggcc acacacccct tccaggaagc
     2881 gttcaagaac taccgcgacc tgctgtacct ctcccgcaac ctgcagcagt ggcaggaaag
     2941 cctgggagtt ctgcgcgaca tgctggccaa ccgtcgcgag gccttcgctg cgcgcctgcc
     3001 gcagattcag gaaaaggagc gcgcgctgaa cctcgccggc ttcgaacagc gcttcgagtc
     3061 cttgcaggtc gaactggatc gcgtcgaacg cgacggtgac gcggcggcat tcgcgaccgt
     3121 ccgcgaacgc gaactgcagg caaggctcga tcgcgtgcgg gagacgctga accttgccgc
     3181 aaaggattcg ggactgacac atgccgaatt ggcgcaggcg aacgaacgcg cccggcgcgc
     3241 cgccggggca ttgctgtggc agcagaacga tcagttcgcg gtgcgcctgt ggagcgcgaa
     3301 gaagggcatg caggaactgc agcgcaacct ggctcaggcc cagcagcgcg acgccgcgct
     3361 ggtggccgcg cagcgggacg aacccgcacg gctcgatgcg ttcgccggtc gtctcgacct
     3421 tctggcagcg cgcgtcgata cgatggttcc gcgtgtcgcg gtgctcacgc aggagcagca
     3481 ggccgccgtg caggaactcg cggtggcgga actcctgcag cagaaggagc ggctggccgc
     3541 gtacggtacg caggcgcgct ttgcggtcgc gcagatagtc gaccgcgcca gcctcgcaaa
     3601 ggagtcggcc cgtgtgcccg cgcagtagtc tggctgtcgc tcgttcgagc gccatcctcg
     3661 cggtggcgct gctcgcggca tgctcgagtc cgttcaagcg ggcacagccc gagccgccga
     3721 cgctgaagtc gctcgcgtcg caccaggtca cggtcaatcc cgatatcggc atcaaagcca
     3781 cagaagagga cgccgcgcgc gcctaccgcg acttcctggc ggcttcgccg cgcgaaccgc
     3841 aaaggcagga ggcacttcgc cgcctcggag acctggaaat ggacctcgtc gactcgcgca
     3901 tggcgaccgg agagacgacc ggatccgagg ccgactacaa ggcagccgtt acgcgatatc
     3961 aggacttcct gaagacttac cccaacgact cgggcaacga ccgagtgctc taccagctcg
     4021 cgcgcgccta cgagttgagc ggcaatctgg agacgtcgct cgcgacgctc gacaaactcg
     4081 tgctgctgta tccgacgacc cgctatcgcg acgaagcgca gttccggcgc ggcgaactga
     4141 tgttcgcgtt gcgcgagtac ccgaaggcgg aggcggcgta cctggtgacg atgcggggcg
     4201 acaagagttc tccctactat gagagatcga tctacatgca cgg
//
LOCUS       DATIRT010000263         1538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1121744, whole genome shotgun sequence.
ACCESSION   DATIRT010000263 DATIRT010000000
VERSION     DATIRT010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121744"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="VLN25_06040"
     CDS             <1..1057
                     /locus_tag="VLN25_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSQ10159.1"
                     /translation="ENTASFKANGTGPYRLRSRDPNVRTTLQRNPGYWGKIEGNVQEV
                     VFTPIGNASTRVAALLSGEIDMMEPVPVQDVARIQASPNLKVMQGPELRTIFLGMDQS
                     RNELLSSSVKGKNPFKDKRVRQAFYQAIDVNAIQKAVMRGASAPTGLMIAPGITGFVP
                     EMNKRLAYDPDASKRLLADAGYPNGFEVWMNCPNDRYVNDEEICKAATAMLAKVGIKV
                     NLTAETKGTYFPKILSRSTSFYLLGWTPSSYDAHNPLFALMATPGAGGQGQFNLGSYS
                     NAKLDELTKAIATETDLKKRNAMIAEAMKVHQDDVGHIPLHQQALAWAMKKNVDVVQL
                     ADNFMPLKWVSVKGAAQ"
     gene            1078..>1538
                     /locus_tag="VLN25_06045"
     CDS             1078..>1538
                     /locus_tag="VLN25_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018026819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSQ10160.1"
                     /translation="MNGLARVWDSDVAYSFRHSPVAMVAALTAFVLIFAAVFAYWVAP
                     HNPFDLATINLLDASLPPSWEAGGNPKFWLGTDDQGRDIFSAVIYGMQISLLIGIASV
                     VLSVVMGVGLGLLSGYVGGRIDAFIMRVCDVMLSFPAILVALLIDGVARAVL"
BASE COUNT          294 a          467 c          507 g          270 t
ORIGIN      
        1 ggagaacacc gcgtcgttca aggcgaacgg caccggcccg tatcgactgc gatcgcgcga
       61 tcccaacgtg cgcacgacgc tgcaacgcaa tcctggctac tggggcaaga tcgaaggtaa
      121 cgtccaggaa gtggtgttca cgccgatcgg caacgcctcg acccgggtcg cagccctgtt
      181 gtcaggcgaa atcgacatga tggagccggt gccggtgcag gacgtggccc gcattcaggc
      241 cagtccgaat ctcaaggtga tgcaggggcc ggaactgcgc acgatcttcc tgggcatgga
      301 tcagtcgcgc aatgaacttc tgtcgtcgag cgtcaagggc aagaatccgt tcaaggacaa
      361 gcgcgtgcgc caggcgttct accaggcgat cgacgtcaac gcgatccaga aggcggtgat
      421 gcgcggcgcc tccgcaccga ccgggctgat gatcgcgccc gggatcaccg ggtttgtgcc
      481 ggagatgaac aagcggcttg cctatgaccc ggacgcttcg aagaggctgc tggccgacgc
      541 cggctatccg aacggctttg aagtctggat gaactgcccg aacgatcgct acgtcaacga
      601 cgaggaaatc tgcaaggctg cgacggccat gctggcgaag gtcggcatca aggtgaatct
      661 caccgccgag accaagggca cgtacttccc gaaaatcctg tcgcgcagta cgtcgttcta
      721 cctgctcggg tggacgccca gcagctatga cgcgcacaac ccgctgttcg cactgatggc
      781 cacgccgggt gccggcggcc aggggcaatt caatctgggc agctacagca acgcgaagct
      841 cgatgaactg accaaggcga tcgcgaccga aacggacctc aagaagcgaa acgctatgat
      901 cgcggaggcg atgaaggtcc accaggatga cgtcggccac attccgctgc accagcaggc
      961 gctggcctgg gcgatgaaga agaacgtcga cgtcgtgcag ctcgcggaca acttcatgcc
     1021 gctgaagtgg gtcagcgtga aaggcgcagc gcagtaaggc cgggccgggg aatcgggatg
     1081 aacggtctgg cccgcgtctg ggacagcgat gtcgcctaca gcttccgcca ctcgccggtg
     1141 gcgatggtgg cggcgctgac ggcgttcgtg ctgatattcg cggccgtgtt cgcctactgg
     1201 gtcgcgcccc ataacccgtt cgatctcgcg acgatcaacc tgctcgacgc ctccctgccg
     1261 ccgtcatggg aggcgggcgg gaatccgaag ttctggctcg ggaccgacga ccaggggcgt
     1321 gacatcttct ccgccgtcat ctacggcatg cagatctcat tgctcatcgg catcgcatca
     1381 gtcgtgctgt cggtggtgat gggcgtcggc ctcggtttgc tgtcgggcta tgtgggcggc
     1441 cgcatcgacg ctttcatcat gcgtgtgtgc gacgtcatgc tgtcgttccc ggccatcctg
     1501 gtagcgctgc tgatcgacgg tgtggcgcgg gccgtgct
//
LOCUS       DATIRT010000264         2133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_728145, whole genome shotgun sequence.
ACCESSION   DATIRT010000264 DATIRT010000000
VERSION     DATIRT010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728145"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VLN25_06050"
     CDS             complement(<1..181)
                     /locus_tag="VLN25_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HSQ10161.1"
                     /translation="MAMVYEGVLLFGVTFAAGLALLSLSGWTAPLSGARRHVLQAALF
                     VVIGAYFVWCWSRSGQ"
     gene            complement(270..761)
                     /locus_tag="VLN25_06055"
     CDS             complement(270..761)
                     /locus_tag="VLN25_06055"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HSQ10162.1"
                     /translation="MRIDMAHRALLAGLVLACTLVWAQGSARPAWSELTTAQREALAP
                     LASDWAALDAERKRKWLEVAAKYPRLSPEGKQKLHERMAEFSRLTPEQKKTARENFRR
                     AYEVPAEQRQATVQKYQELPEDKKRELANRAASKGDAQRAPPLDSRVPPAKPPAKTEP
                     QKQ"
     gene            complement(773..1183)
                     /locus_tag="VLN25_06060"
     CDS             complement(773..1183)
                     /locus_tag="VLN25_06060"
                     /inference="COORDINATES: protein motif:HMM:NF023699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HSQ10163.1"
                     /translation="MNQDEFGYRIRQALDEGLDRLDYRTTYRLEQARLVALARQPAAQ
                     AERGWAPALQTAGGPPVDRESAGWARRLGLAAPLLALVIGFVGINQYQESEESRRISE
                     LANMDFAVLLDDVPLETYADKGFGALLQGESDEL"
     gene            complement(1180..1743)
                     /locus_tag="VLN25_06065"
     CDS             complement(1180..1743)
                     /locus_tag="VLN25_06065"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HSQ10164.1"
                     /translation="MATRDELSAFLASVERRAFKQAQFAVRDSDAALDIVQDAMIKLS
                     ENYSDRPAAEFPLLFTRILQNVIHDHFRRQKVRSTWVTLFSALTPAGDDDADPLETLE
                     ALAGSDGAESAGDKVERAQVVAIIEEEVGRLPARQREAFLMRYWEDMDVAETAAAMGC
                     SEGSVKTHCSRATHALAKALRARGITL"
BASE COUNT          365 a          701 c          694 g          373 t
ORIGIN      
        1 tctggccgga acggctccag caccagacaa aataggcccc gatgacgacg aacagtgccg
       61 cttgcaggac gtgccggcgt gcaccgctca ggggcgcagt ccagccggac agtgagagaa
      121 gggcgagccc ggcggcaaag gtcacgccaa acagcagcac gccttcgtag accatcgcca
      181 tgacccgcag gcttaggctc gccgggcggc ctggcgtggc gggcacactc accgtcgtca
      241 aaggctcggc gacgtcacgg gccgagacgt cactgcttct gtggttccgt cttggccggc
      301 ggcttggccg gaggaacgcg gctgtcgagc gggggcgcgc gctgtgcatc gcctttgctc
      361 gcagcgcggt ttgccagttc ccgcttcttg tcttcgggca gttcctggta cttctgcacc
      421 gtggcctggc gttgctcggc cggcacctcg tacgcacgcc ggaagttctc gcgcgcggtc
      481 ttcttctgct cgggcgtcag gcgcgagaac tcggccatgc gctcgtgcag cttctgcttg
      541 ccttcgggcg acagccgcgg gtacttggcg gcgacttcga gccacttgcg cttgcgctcg
      601 gcgtccagcg cggcccagtc cgacgccagg ggggcaagcg cttcgcgctg cgcggtcgtc
      661 aattcactcc aggccggccg ggcgctgccc tgggcccaga cgagagtgca cgcaagcacg
      721 agcccggcaa ggagggcgcg atgcgccatg tcgatacgca cgcggcctcc gtctaaagtt
      781 cgtctgattc gccctggagc agggcgccga accccttgtc cgcgtaggtt tcaagcggaa
      841 cgtcgtccag caggacggcg aagtccatgt tggcgagttc cgagatgcga cgcgactcct
      901 cggactcctg gtactggttg atgccgacaa agccgataac cagggccagc agcggagcgg
      961 ccagtcccag ccggcgtgcc cagcctgcgg actcacgatc gaccggcggg ccgccggccg
     1021 tctgcagggc tggcgcccag ccacgctcgg cctgcgccgc gggctggcgt gccagcgcca
     1081 ccagtcgggc ctgctccagc cggtatgtag ttcgatagtc gagccgatcc aggccctcgt
     1141 cgagcgcctg gcggatccga tacccaaatt cgtcctggtt catagcgtga tgcctcgtgc
     1201 gcgcagggcc ttggccagcg catgcgtggc ccgtgagcag tgcgtcttga cgctgccttc
     1261 ggaacacccc attgccgcgg cggtttccgc cacgtccatg tcttcccagt aacgcatcag
     1321 gaaggcttcc cgttgacggg ccggaagccg gccgacttct tcctcaatga tcgccaccac
     1381 ctgcgcccgt tcaaccttgt ctccggcact ttccgcgcca tcggagccgg cgagcgcctc
     1441 cagcgtttcc agcgggtccg cgtcatcgtc gccggcaggc gtgagcgccg aaaacagcgt
     1501 gacccacgtc gatcggacct tctgacgcct gaaatgatcg tggatgacgt tctggagtat
     1561 gcgggtgaaa agcaatggaa actcggccgc aggtcgatcg ctgtagttct ccgacaactt
     1621 gatcatcgcg tcctggacga tatccagcgc ggcatcgctg tcacgcactg cgaactgcgc
     1681 ctgcttgaag gcgcggcgct cgacgctggc gagaaacgcg gaaagctcgt cgcgggtggc
     1741 cattagagga gctgtcgggg caaagccgcg cgccagcccg cggagaggaa aacggcggcg
     1801 gatgctatca gacgaagttt cactcggtac acacgatggc gcattgtgcc ggccgatcca
     1861 agagcagtgg gcaacctgcc ggcgaggcgg tttgcagaat gttgtgcgct gcagtattgt
     1921 tttcggttct tgcaaaacga gaacacgtct ttagcgacac agcctatccc gttgtaaccg
     1981 cgtaaagcgc cccccgccgc accgcgcgcc acaagcgtgc atggacggca tccgatcaat
     2041 ttcccgtgga ctttgagagg agttagcgaa atgcaactca ccggcgcaga aatcgttgta
     2101 cgagcgctgc aggacgaagg cgtcacacac gtc
//
LOCUS       DATIRT010000265         2834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1711663, whole genome shotgun sequence.
ACCESSION   DATIRT010000265 DATIRT010000000
VERSION     DATIRT010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1711663"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VLN25_06070"
     CDS             <1..801
                     /locus_tag="VLN25_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSQ10165.1"
                     /translation="GKTEVAKQLAFTMGIELIRFDMSEYMERHAVSRLIGAPPGYVGF
                     DQGGLLTEAISKKPHAVLLLDEIEKAHPDIFNILLQVMDHGTLTDNNGRKADFRNVII
                     VMTTNAGAETLQRTAIGFSNTREAGDEMADIKRMFTPEFRNRLDAIISFKALNEEVVL
                     RVVDKFLMQLEDQLHEKKVDIVFTDVLRAHLAKKGFDPVMGARPMARLIQDTIRKALA
                     DELLFGKLVNGGRVEVDVDADGNVKLDFPPADSGDAPRREQGEPVLSE"
     gene            946..1263
                     /locus_tag="VLN25_06075"
     CDS             946..1263
                     /locus_tag="VLN25_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSQ10166.1"
                     /translation="MKTTITVTGALLALATSTVLAQAPALPANAGYLASNCANCHGTT
                     GIAKGAMPSLAGQQKTYIVDQMRAFRDGKRPATIMHQLAKGYSDQQIELVADFFSRQT
                     PAR"
     gene            1274..2566
                     /locus_tag="VLN25_06080"
     CDS             1274..2566
                     /locus_tag="VLN25_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSQ10167.1"
                     /translation="MDTPNLSRRDLLKALSAAAATSALSACATMDSKKPIGKVVVIGA
                     GYGGATAAKYLRLWSEGAIDVTLVEPNEAFISCPISNLVLAGSRTMADITVSYAGLDK
                     HGVKRARDTVTAIDADKKQVRLASGGALPYDRLVVSPGIDFMYERLQGWSAAAEGTVL
                     HAWKAGPQTLALRRQLTELKDGGVVVMAIPLAPYRCPPGPYERACQIAWYLKQSKPKS
                     KLIVLDANPDYVSKKPLFSRVFSEDYKGIVDYRVASPVTEIDPKTGTLVTEIGDKVRG
                     DVVNFIPPQRAANIARDAGLITSNNRWCEVDWTTLESIKVKGVHVLGDATLSAPAMPK
                     SGHMANQHGKAAAAAIVELLNGRAVTPPTMANTCYSFIDDKNAVHVASVHRWVTEKKT
                     LEPVAGAGGISSQDRASWALEGAYANGWAQTIWADMLT"
BASE COUNT          540 a          898 c          924 g          472 t
ORIGIN      
        1 ggcaagaccg aggtcgccaa gcaactcgcc ttcacgatgg gcatcgagct gatccgtttc
       61 gacatgtcgg agtacatgga acggcatgcc gtcagtcgcc tgatcggcgc ccccccgggc
      121 tacgtcggct tcgaccaggg cggactgctg actgaagcca tcagcaagaa gccgcatgcg
      181 gtcctgctgc tcgacgagat cgagaaggcg catccggaca tcttcaacat cctgctgcag
      241 gtgatggatc acggcacgct gaccgacaac aacggccgca aggcggattt ccgcaacgtg
      301 atcatcgtga tgacgaccaa tgcgggcgcc gagacgctgc agaggaccgc catcggattc
      361 tcaaacacgc gcgaggccgg tgacgagatg gccgatatca agcgcatgtt cacgccggag
      421 ttccgcaacc ggctcgatgc gatcatctcg ttcaaggcgc tcaacgaaga agtggtgctg
      481 cgcgtggtcg acaagttcct gatgcagctc gaggaccagc tgcacgagaa gaaagtcgac
      541 atcgtcttca ccgatgtgct gcgtgcgcac ctggcgaaga aaggtttcga tccggtgatg
      601 ggcgcgcgtc cgatggcgcg gctgatccag gacacgatcc gcaaggcact ggccgacgaa
      661 cttctgtttg gaaagctggt gaacggcggc cgtgtggagg ttgatgtcga tgccgacggc
      721 aacgtgaagc tcgatttccc gcctgcggac agcggtgatg cgccgcggcg cgaacagggt
      781 gaaccggttc tgtccgagta gcggggcgtc cggcttctaa gcaaatgaga agcctgtatt
      841 gagaaaccac cgccgcgcgc ggacaaccgg ttgacgtccg cgccgaaact gcctccaatg
      901 gagcccgcag agaccctgcg ggctttttcg ttcaaggaga ccccattgaa aacaaccatc
      961 acggtcaccg gcgcgctgct ggcgctcgcg acatccacgg tgctcgcgca ggccccggct
     1021 ttgcccgcca atgcgggcta cctggcatcc aactgtgcga actgccacgg cacgaccggc
     1081 attgccaaag gtgcgatgcc cagcctggcc ggccagcaga agacctacat cgtcgaccag
     1141 atgagggcgt tccgtgacgg caagcgtccg gccacgatca tgcatcagct cgccaagggc
     1201 tactccgacc agcagatcga actggtcgcc gactttttct cgcggcagac gccggcacgc
     1261 tagggacgga ctcatggaca ccccaaacct ctctcgtcgt gacctgttga aagccctgtc
     1321 ggcggccgct gcgacctcgg ccctgtcggc ctgcgcgacc atggacagca agaagccgat
     1381 cggcaaggtg gtcgtgatcg gtgccggtta cggcggcgcg acggcggcca agtatctgcg
     1441 cctgtggtcc gagggcgcga tcgacgtcac cctggtcgag ccgaatgaag ccttcatttc
     1501 gtgcccgatt tccaatctgg tcctggccgg gtcgcggacg atggccgaca tcacggtcag
     1561 ctatgccggg ctggacaaac acggtgtcaa gcgcgcgcgc gacaccgtga cggcgatcga
     1621 tgctgacaag aaacaggtcc ggctcgcgtc cggcggcgcg ctcccctatg accggctggt
     1681 cgtttcaccc gggatcgact tcatgtacga acgcctgcaa ggctggtcgg cagcggccga
     1741 agggaccgtg ttgcacgcct ggaaggcggg gccgcagacc ctggctctgc gccggcaact
     1801 caccgaactg aaggacggtg gcgtggtcgt gatggcgatt ccgctggcgc cgtatcgttg
     1861 cccgcccgga ccgtatgagc gcgcatgcca gattgcgtgg tacctgaagc aatccaagcc
     1921 gaagagcaaa ctgatcgtgc tcgacgcgaa cccggactac gtgtcgaaga agccgctgtt
     1981 ctcgcgcgtg ttcagcgagg actacaaagg catcgtcgac tatcgcgtcg cctccccggt
     2041 cacggaaatc gaccctaaga ccggcacgct cgtcaccgag atcggggaca aggtgcgcgg
     2101 cgatgtcgtc aatttcatcc cgccgcagcg cgccgcgaac attgcgcggg acgctgggct
     2161 gatcacgagc aacaaccgct ggtgcgaggt cgactggacc acgctggagt cgatcaaggt
     2221 caagggcgtc cacgtgcttg gagacgcgac gctgtccgcc ccggcgatgc cgaagtcggg
     2281 ccacatggcg aaccagcacg gcaaggcggc ggccgccgcg atcgtcgagt tgctcaacgg
     2341 tcgcgcggtc actccgccga cgatggccaa cacgtgctac agcttcatcg acgacaagaa
     2401 cgccgtgcac gtggccagcg tgcaccgctg ggtaacggag aagaaaacgc tcgagcccgt
     2461 tgccggcgcc ggtggcatct cgtcgcagga tcgcgcgtcc tgggcgctcg aaggtgccta
     2521 tgccaacggc tgggcgcaga cgatctgggc ggatatgttg acgtagcgcc tcgggtgtac
     2581 gcgatcgctt cgcgatcgca cgtgcaccct cggcggtacg cgcgctctgc gagcgcttgg
     2641 atcagtcggt cggctccggg cggccgcgcg tcgaccgaag gacaaataca acgaaaggtg
     2701 gcgccgcggc gccacctttt gccttccgcg gtgagcgttc tcgatgtccc tgccgtcagc
     2761 gggtgctggg gtcgctttct cgaaaagcga tcgtgcttgc ctgctcgggc gggcggccca
     2821 ccttgtccgc gtag
//
LOCUS       DATIRT010000266         2773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1809791, whole genome shotgun sequence.
ACCESSION   DATIRT010000266 DATIRT010000000
VERSION     DATIRT010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1809791"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VLN25_06085"
     CDS             complement(<1..350)
                     /locus_tag="VLN25_06085"
                     /inference="COORDINATES: protein motif:HMM:NF013858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HSQ10168.1"
                     /translation="MKQSKTSTPSPATLLALLHLASPALPVGGYAYSQGLESAIDEGH
                     VHDAASAQCWIDDLLTLVIARLDAPMWLRVHDATLAGDEDAVTRWNRELLATRETAEL
                     RAESLQMGASLLRV"
     gene            complement(347..889)
                     /gene="ureE"
                     /locus_tag="VLN25_06090"
     CDS             complement(347..889)
                     /gene="ureE"
                     /locus_tag="VLN25_06090"
                     /inference="COORDINATES: protein motif:HMM:NF009751.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HSQ10169.1"
                     /translation="MIRLTAVARAPLTPEQAARTPRLPLAYHERVRSRLAAMLPDGQG
                     VAITLPRGSVMRGGTVLTGDDGACVIVDALAEPVAKVTAPSPLALMRATYHLANRHVP
                     AQLASDHVLIERDPVLEHMLEGLGATVQHIEAPFDPEAGAYDGQNAHGHVHASEVDEV
                     SATVGEQLSIAAHRARSGAS"
     gene            complement(903..2615)
                     /gene="ureC"
                     /locus_tag="VLN25_06095"
     CDS             complement(903..2615)
                     /gene="ureC"
                     /locus_tag="VLN25_06095"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HSQ10170.1"
                     /translation="MKISRQAYAEMFGPTVSDRVRLADTALIAQVEKDHTVYGEEVKF
                     GGGKVIRDGMGQSQRMSKDCADTVITNALIIDHWGIVKADIGLKGGRISGIGKAGNPD
                     IQPAVTIVIGPGTEIIAGEGIIVTAGGIDSHIHFICPQQIDDALYSGITTLLGGGTGP
                     ATGTFATTVTPGPWHMERMLQAADAFPMNLGFLGKGNASLVPPLLEQIEAGAIGLKLH
                     EDWGTTPAAIDAALTAADKTDTQFAIHTDTLNEAGFVEASIKAFKGRVIHTYHTEGAG
                     GGHAPDIIKVCGELNVLPSSTNPTRPYTVNTLDEHLDMLMVCHHLDAGIAEDISFAES
                     RIRKETIAAEDILHDIGAFSMISSDSQAMGRVGEVITRTWQTAHKMKQQRGSLSEKSF
                     SDPSAHDNARIKRYVAKYTINPAIAHGIAHEVGSVEVGKWADLVLWKPAFFGVKPFLI
                     LKGGTIAAAAMGDPNASIPTPQPVHYRPQFGAFGGSLAKSSLTFVSQAALRKRIPEKY
                     GLQKSVVAVKNVRKLSKRDMVHNTLTPKVTVDPETYQVHADGVLLDCAPASELPLAQR
                     YFLF"
     gene            complement(2612..>2773)
                     /locus_tag="VLN25_06100"
     CDS             complement(2612..>2773)
                     /locus_tag="VLN25_06100"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HSQ10171.1"
                     /translation="RFEPGQEREVELVALAGDRIVYGFNAKVMGRLDASTRARKSAKV
                     KSRAKASRK"
BASE COUNT          428 a          896 c          925 g          524 t
ORIGIN      
        1 aagacgcgca gcagcgacgc gcccatttgc agcgactcgg cgcgcagttc cgcggtttcg
       61 cgcgtggcca gcagttcacg gttccagcga gtgaccgcat cctcgtcgcc ggccagcgtt
      121 gcgtcatgga ccctgagcca catgggcgcg tcgagccggg cgatcacgag tgtcaacaga
      181 tcgtcgatcc agcactgagc gctcgccgcg tcgtgcacat gcccctcatc gatcgccgac
      241 tcgagtccct gtgaataggc gtagccgccg accggcaacg ccgggcttgc cagatgcagc
      301 agcgcgagca aagtggccgg cgagggcgtc gatgtcttgc tctgcttcat gacgctccgc
      361 tgcgcgctcg atgggccgcg atcgacagct gctcgccgac ggtcgcggag acttcgtcga
      421 cctcgctggc gtggacgtga ccgtgcgcgt tctgcccgtc gtaggcaccg gcctccggat
      481 cgaagggcgc ctcgatgtgc tgcaccgtgg cgccaagccc ctcaagcatg tgctccagca
      541 ccggatcgcg ttcgatcagc acatggtcgc tcgcgagctg agccgggacg tggcgatttg
      601 ccaggtgata ggtcgcacgc atcagcgcca gcggcgacgg ggcggtgact ttcgccacgg
      661 gttccgccag cgcgtccacg atcacgcagg cgccgtcgtc gccggtcagg acggtgccgc
      721 cacgcatcac ggagccgcgc ggcaacgtga tggccacacc ctgaccgtcg ggcagcatcg
      781 cggcgagtcg gcttctgacg cgctcgtgat aggcgagcgg caggcgcggc gtgcgcgccg
      841 cctgctccgg agtcagaggt gcacgggcga cggcggtgag ccggatcatc agatcgaacc
      901 gttcagaaga gaaagtaccg ctgcgccagc ggcagttcgc tggccggcgc gcagtctagc
      961 agcacaccgt cggcgtggac ctggtaggtc tccgggtcga cagtcacctt gggcgtcaac
     1021 gtgttgtgca ccatgtcgcg cttgctgagc ttgcggacat tcttcaccgc gacgaccgac
     1081 ttctgcagtc cgtacttctc gggaatgcgc ttcctaagtg cggcctgcga gacaaatgtc
     1141 agcgaactct tcgccagcga tccgccgaag gcaccgaact gcggccggta gtgcaccggc
     1201 tgcggcgtcg ggatcgacgc gttggggtca cccatcgcgg ccgccgcgat ggtgccgccc
     1261 ttgaggatca ggaacggttt gacgccgaag aaagccggct tccacagcac gaggtcggcc
     1321 cacttgccga cttcgacgct acccacctcg tgcgcaatgc cgtgcgcgat cgcgggattg
     1381 atcgtgtact tggccacata gcgcttgatg cgcgcgttgt cgtgcgccga gggatcggag
     1441 aagcttttct ccgaaagtga cccgcgctgc tgcttcatct tgtgcgcggt ctgccacgtg
     1501 cgcgtgatga cttcgccgac gcggcccatc gcctgcgagt ccgatgaaat catcgagaac
     1561 gcgccgatgt cgtgcaggat gtcctcggcg gcaatagttt ccttgcggat gcggctctcg
     1621 gcaaaggaga tgtcctcggc gatgccggca tcgaggtggt ggcagaccat cagcatgtcg
     1681 aggtgctcgt cgagcgtgtt gacggtgtag ggccgggtcg ggttggtcga cgacggcagc
     1741 acgttcagct cgccgcacac cttgatgatg tcgggcgcgt gcccgccccc cgcaccctcg
     1801 gtgtgatagg tgtggatcac gcggcccttg aacgccttga tgctggcttc gacgaagccg
     1861 gcttcgttca gcgtgtcggt gtggatcgcg aactgcgtgt ccgtcttgtc ggcggcggtg
     1921 agcgcggcgt cgatcgcggc gggcgtggtg ccccagtctt cgtgcagctt caggccgata
     1981 gcgccggcct cgatctgctc cagcaagggc ggcacgagcg acgcattgcc cttgccgaga
     2041 aagccgaggt tcattgggaa cgcgtcggcg gcttgaagca tgcgctccat gtgccaaggg
     2101 cctggcgtga ccgtggtcgc aaaagtgccg gttgcgggtc cggtgccgcc cccgagcagc
     2161 gtcgtgatgc cgctataaag cgcatcgtcg atctgctgcg ggcagatgaa gtggatgtgt
     2221 gaatcgatgc cgccggcggt gacgataatg ccctcgccgg caatgatttc ggtgccgggc
     2281 ccgatgacga tcgtcacggc gggctggatg tccggattgc cggccttgcc gatgccgctg
     2341 atgcgtccgc ccttcagtcc aatatcagcc ttgacgatgc cccagtggtc gatgatgagc
     2401 gcattggtga tcaccgtgtc ggcacagtcc ttcgacatgc gctggctctg gcccatgcca
     2461 tcgcggatca ccttgcctcc gccgaacttc acttcctcgc cgtagacggt gtggtccttc
     2521 tcgacctgcg cgatcagcgc cgtgtcggcg agccgcacgc ggtcgctgac cgtggggccg
     2581 aacatttcgg cataggcctg gcgagagatc ttcatttgcg gctcgccttc gcgcgtgact
     2641 tcactttcgc cgactttctt gcacgtgtcg acgcgtcgag ccttcccatc accttcgcgt
     2701 tgaacccgta gacgatgcgg tcgcccgcca gcgccaccag ttcgacttcg cgttcctgtc
     2761 cgggctcgaa gcg
//
LOCUS       DATIRT010000267         2078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1514736, whole genome shotgun sequence.
ACCESSION   DATIRT010000267 DATIRT010000000
VERSION     DATIRT010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514736"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VLN25_06105"
     CDS             <1..253
                     /locus_tag="VLN25_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HSQ10172.1"
                     /translation="VEQGGNCALSELGRTVTKYGVHIIGQPNIPALVPTDTSALYARN
                     VLDFLKLVVDKDGNFTLPAEDDIVAACLMCAGGEIKRKN"
     gene            277..591
                     /locus_tag="VLN25_06110"
     CDS             277..591
                     /locus_tag="VLN25_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HSQ10173.1"
                     /translation="MEAVSPIVINLIIFVLAIYVGYHVVWNVTPALHTPLMSVTNAIS
                     AIIIVGAMLAAALTETGLGKTMGVLAVALAAVNVFGGFLVTRRMLEMFKKKEPKAGSA
                     KH"
     gene            601..2031
                     /locus_tag="VLN25_06115"
     CDS             601..2031
                     /locus_tag="VLN25_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HSQ10174.1"
                     /translation="MALSMNVVILLYLVASVFFIQALKGLSHPSTSRQGNAFGMIGMA
                     IAMVTTVALIYKTAPQLATAGMVWVLVGLVVGGAIGTTIAFKVEMTKMPEMVAFMHSM
                     IGLAAVFIAIAAVAEPGAFGIADSKGAIPVGNRIELFIGAFVGAITFSGSVIAFGKLS
                     GKYKFRLFQGAPVVFSGQHMLNLALAIAMLVFGMWFAFTQDWFAFWAMLTIAFVLGVL
                     IIIPIGGADMPVVVSMLNSYSGWAAAGIGFSLNNFMLIVAGSLVGSSGAILSYIMCKA
                     MNRSFFNVILGGFGGEVAAATGAAEQRPVRSGSPDDAAFLMENAETVIIVPGYGLAVA
                     RAQHPLKELTDKLIRKGVTVKYAIHPVAGRMPGHMNVLLAEAEVPYDQVFEMEDINGE
                     FGEADVALILGANDVVNPAAKDPKSAIAGMPILEAYKAKQVIVNKRSIASGYAGLDNE
                     LFYMPKTMMVFGDAKKVVEQMSKAIG"
BASE COUNT          380 a          612 c          685 g          401 t
ORIGIN      
        1 ggtcgagcag ggcggcaact gcgcgctctc ggagctcggc aggacggtga cgaagtacgg
       61 cgtgcacatc atcggccagc ccaacatccc ggcgctggtc cccactgata cgtcggcgct
      121 ctatgcgcgc aacgtgctcg acttcctcaa gcttgtggtc gacaaggacg gcaatttcac
      181 gctgcctgcc gaggatgaca tcgtggcggc ctgcctgatg tgcgcgggcg gcgaaatcaa
      241 gcgcaagaac tgaacaagag acacaccagg actcccatgg aagccgttag cccgattgtc
      301 atcaacctga tcatcttcgt gctggccatc tacgtcggct accacgtcgt atggaatgtc
      361 acgccggccc tgcacacgcc gctcatgtcg gtaacgaacg caatctccgc catcatcatc
      421 gttggcgcga tgctggcagc cgcactgacc gagaccggac tcggcaagac catgggcgtg
      481 ctcgcggtcg cgctggccgc ggtcaacgtc ttcggcggct tcctggtcac gcgaaggatg
      541 ctcgagatgt tcaagaagaa ggagccgaag gccggctccg cgaagcacta ggggcgcgcg
      601 atggcactca gcatgaacgt cgtcatcctg ctgtacctcg ttgcgtcggt gttcttcatc
      661 caggcgctga agggactgtc gcacccgagc acgtcgcgcc agggcaatgc cttcggcatg
      721 atcgggatgg cgattgcgat ggtcaccacg gtcgcgctga tctacaagac ggctccgcag
      781 cttgcgaccg ccggcatggt ctgggtactg gtcgggttgg tcgtcggcgg tgcgatcggc
      841 accacgatcg cgttcaaggt cgagatgacc aagatgccgg agatggtcgc cttcatgcac
      901 agcatgatcg gcctggcggc cgtgttcatc gcgattgcgg cggtggccga gccgggcgcg
      961 ttcggcatag ccgattcaaa gggcgcgatc cccgtcggca accgcatcga actcttcatc
     1021 ggcgcgtttg taggcgcgat cacgttctcg ggatcggtga tcgccttcgg caaactgtct
     1081 ggcaagtaca agttccggct gtttcagggc gcgccggtgg tgttttcggg ccagcacatg
     1141 ctgaatctcg cgctcgcgat cgcgatgctg gtgttcggga tgtggtttgc gttcacgcag
     1201 gactggttcg cgttctgggc gatgctcacg atcgccttcg tgctcggtgt gctgatcatc
     1261 atcccgatcg gcggagctga catgccggtc gtggtgtcaa tgctgaacag ctactcgggc
     1321 tgggcggccg cgggcattgg cttctccctg aacaacttca tgctgatcgt cgccggctcg
     1381 ctggtcggat cgtcgggtgc gatcctcagc tacatcatgt gcaaggcgat gaaccgctcg
     1441 ttcttcaacg tgatcctcgg gggctttggc ggcgaagtcg cggcggccac cggcgcggcc
     1501 gagcagcggc ctgtgcgctc gggatcgccg gatgacgcag ctttcctgat ggaaaacgcc
     1561 gagaccgtca tcatcgtgcc gggctatggt ctcgcggtcg cgcgcgcgca gcacccgttg
     1621 aaggaactga cggacaagct catcaggaaa ggcgtgacgg tgaagtacgc aattcatccg
     1681 gtcgcgggcc gcatgccggg tcacatgaac gtactgctgg cggaggccga ggtgccgtac
     1741 gaccaggtgt tcgagatgga ggacatcaac ggcgagttcg gcgaggcgga tgtcgcgctg
     1801 atcctcggtg ccaacgacgt cgtcaacccg gcagcgaagg acccgaagtc ggccatcgcc
     1861 ggcatgccaa tcctggaggc ctacaaggcg aagcaggtga tcgtcaacaa gcgctcgata
     1921 gcgtcgggct atgccggcct cgacaatgaa cttttctata tgccgaagac gatgatggtc
     1981 tttggcgatg cgaagaaggt cgtcgagcag atgagcaagg ccataggata gaacgatcga
     2041 ggtagggtct atgcagtcgg acgcgctgct gctcgtcg
//
LOCUS       DATIRT010000268         5176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1122037, whole genome shotgun sequence.
ACCESSION   DATIRT010000268 DATIRT010000000
VERSION     DATIRT010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122037"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..633
                     /locus_tag="VLN25_06120"
     CDS             28..633
                     /locus_tag="VLN25_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HSQ10175.1"
                     /translation="MQPTTPATEGPQEHVLTASGEFDVHDEPIDLSIYRFEDWISFAF
                     FWLLAGTVFLQFFTRYALNDSAGWTEEIARYLLVCTVFIGAAVSVRKNNHIQVDFFYR
                     ILPKRLMRVMSSLVDVARIAFFGYAAWLTYLLMQRIGSQRMAVVDLPIGLMYSVVLAG
                     FMLMTWRAIDVARLNWRRGVSVLEQPELADGHGELPAATGR"
     gene            636..2042
                     /locus_tag="VLN25_06125"
     CDS             636..2042
                     /locus_tag="VLN25_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HSQ10176.1"
                     /translation="MGPGAFKLVFLGLMGSGVPVAIAMAGSSLIFVMATGIVPDFVVI
                     HRMVNGIDSFPLLAVPFFILAGNLMNSAGITNRIYNFALALVGWLRGGLGHVNVLGSM
                     IFAGMSGTAIGDAAGLGTIEIKAMKDHGYSAEFAVGVTAASATLGPIIPPSLPFVIYA
                     MFANVSVGALFLAGILPGAVMALLMMLTVAYFAHKNKWGGDIRFEWARIIKALTELLV
                     VVLFPVVIWALTTKAGLPHVPTVTATLVVLLLADRVFRFNAVLPIMTPVLLIGGMTAG
                     VFTATEGAIAACVWSLFLGIFWFRSLNWKLLIKVSMDTIETTSIVLLIVAAASIFGWM
                     LTVTKTTEMIAGWVLAFTTDPWMFLLLANLLMLFVGCFLEPTAAITILVPILLPIVHQ
                     LGIDPVHFGLIMVLNLMIGLLHPPMGLVLFVLARVAKLSIERTTMAILPWLIPLLVSL
                     GLVTYVPTISLWLPRQFL"
     gene            2057..3601
                     /locus_tag="VLN25_06130"
                     /pseudo
     CDS             2057..3601
                     /locus_tag="VLN25_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
     gene            3644..4645
                     /locus_tag="VLN25_06135"
     CDS             3644..4645
                     /locus_tag="VLN25_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid TRAP transporter substrate-binding
                     protein SiaP"
                     /protein_id="HSQ10177.1"
                     /translation="MKDSIFAQFRIGHARWVVGVAAALLAVGGVSAQTKLKFAHVYEV
                     SEPYHTAAVWAAGEIGKRTSNRYTMEVFPASTLGKETDINQGLTLGTVDVIYTGQLFA
                     GRAYGPIAIGGAPYMFRDFNHWKAFSTSPLFGELAEGYRGKSGGNKVLAITYYGERQV
                     TSNKPINKPEDMKGLKIRVPDAPLYTMFPRAVGANPTPIAFAEVYLALQNGTVDAQEN
                     PLPTIDAKKFYEVQKYIVLTGHITDALLTIVGGPTWSKLSDVDKKIFEEVFREAAVRA
                     TSQIIEIEAKLGAEFAKRGKEVVKVDRAPFRESVAKMHNGPDATWDKATYDKLQALK"
     gene            complement(4830..>5176)
                     /locus_tag="VLN25_06140"
     CDS             complement(4830..>5176)
                     /locus_tag="VLN25_06140"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HSQ10178.1"
                     /translation="ARQRGDLQSAVMAVRASNLPDILMPRYLIERTFPDGLSIPMNDE
                     GRRVVAGVVATNADHGVTWVHSYVTTDRKQTFCIYDGPAPETIRKVAERNGLPIGRIT
                     EVSVLDPYFYRP"
BASE COUNT          937 a         1576 c         1678 g          985 t
ORIGIN      
        1 ggtgaacctg caccgcaacg aacgcgcatg caaccgacga cgccggccac cgaaggaccg
       61 caggaacacg tgctcaccgc cagcggcgag ttcgacgtcc atgacgagcc gatcgacctc
      121 agcatctacc gtttcgagga ctggatctcg ttcgccttct tctggctgct cgccggcacc
      181 gtctttctgc agttcttcac acgctatgcc ctcaacgact cggccggctg gaccgaggag
      241 atcgcgcgct acctgctggt ctgcacggtg ttcatcggcg ccgcagtcag tgtgcgcaag
      301 aacaaccata ttcaggtcga ctttttctac cgcatactgc cgaagcggct gatgcgcgtg
      361 atgtcgtcgc tggtcgacgt ggcgcgtatc gcattcttcg gctacgcggc ctggctgacc
      421 tatctcctga tgcagcgcat cggcagccag cgcatggcgg tcgtcgatct gccgattggc
      481 ctcatgtatt ccgttgtact agccggattc atgctgatga cgtggcgtgc gatcgatgtc
      541 gcgcggctca actggaggcg cggcgtctcg gtgctcgaac agcctgagct tgccgatgga
      601 cacggcgagc ttcccgccgc gacgggtcgt tagtcgtggg cccgggcgca ttcaagctgg
      661 tgtttctcgg cctgatggga tcaggcgttc ccgtggccat cgcgatggcg ggctcatcgc
      721 tgatcttcgt gatggcgacc ggcatcgtgc cggacttcgt cgtcatccac cgcatggtga
      781 acggtatcga ttcgtttccg ctgctggcgg tgcctttttt catcctcgcc ggcaacctga
      841 tgaactcggc ggggatcaca aaccgcatct acaactttgc gcttgccctg gtcggctggc
      901 tgcgcggcgg gctaggccac gtgaacgtgc tgggctcgat gatcttcgcg ggcatgtcgg
      961 gcaccgccat tggcgatgcc gccgggctgg gcacaatcga gatcaaggcc atgaaggacc
     1021 atggctacag cgccgagttt gccgtcggcg tcaccgccgc gtcggccacc ctcggaccca
     1081 tcattccgcc atcgttgccg ttcgtgatct atgcaatgtt cgccaatgtc tcggtcggtg
     1141 cgctgtttct ggcgggcatc ctgccgggcg cggtgatggc gctgttgatg atgctcacgg
     1201 tggcgtactt cgcgcacaag aacaagtggg gcggggacat ccgcttcgag tgggcacgca
     1261 tcatcaaggc gctgacggaa ttgctggtcg tggtgctgtt cccggtcgtc atctgggcgt
     1321 tgaccaccaa ggcgggactg ccgcatgtac ccacggtgac cgccacgctg gtggtgttgc
     1381 tgctcgccga ccgcgtcttc cggttcaacg cggtgctgcc gatcatgacg ccggtgctgc
     1441 tgatcggcgg catgacggcc ggagtattca ccgccaccga aggggcgatc gcggcgtgcg
     1501 tgtggtcgct cttcctcggc atcttctggt ttcgctcgct caactggaag ttgctgatca
     1561 aggtctcgat ggatacgatc gagaccacgt cgatcgtgct gctgatcgtg gcggccgcgt
     1621 cgatcttcgg ctggatgctg acggtgacca agaccaccga gatgatcgcg ggttgggtgc
     1681 tggccttcac gaccgacccg tggatgttcc tgctgctggc caacctcctg atgctgttcg
     1741 tcggctgctt tctcgagccg accgccgcta tcactatcct ggtgccgatc ctgctgccca
     1801 tcgtccatca attgggcatt gaccccgtcc acttcgggct catcatggtg ttgaacctga
     1861 tgatcggcct gctgcatccg ccgatgggtc tggtgctgtt cgtattggcg cgcgtggcga
     1921 agctttcgat cgagcgcacg acgatggcga tcctgccgtg gctgattccg ctgctggtga
     1981 gcctcggtct ggtgacctac gtgccgacga tcagcctgtg gctgccgcgg cagtttctgt
     2041 agtgggagga ggggcaatgc tgcaggaagt tccgctggtc cttcgtctca acccggccga
     2101 cgatgtcgtg atcgcacgac gtcagctggt ggcgggcacg ctgatcaagt ccgaggggat
     2161 caccatcagc gggctcgtgc ctcccggcca caaggtcgcg acgcgggcgt tggcggtggg
     2221 tgctccggtg cgacgctaca accagatcat cggctttgcg acgcgcgaaa tccggccagg
     2281 cgagcacgtg catctgcaca acctaggcat aggcgccgag cacggtgcct tcgaacggga
     2341 ctacgcgttc tgcgcagacg tgcagtcgac ggcgaccgtc gcaacgcccg caactttcat
     2401 gggcatcgta cggcccgacg gacgggtggc gacgcgcaac tacatcgcga tcctctcgag
     2461 tgtgaattgt tcagccacgg tcgcgcgggc gattgccgac cgcttccgcc gggatatctt
     2521 tcccgacgct cttgcggact ttcccaatgt cgacggcgtc atcgcactga cgcacggcac
     2581 cggctgcggc atggacatgg gcgagagcat gcaggtcctg cgccgcacga tggccggtta
     2641 cgcccgccat ccgaacatgg cctcagtgat tttcgtcggg ctgggctgcg aagccaacca
     2701 gatcacgtcg ctgttcggcg cggaaagcct gaaagaaagt gaacggatcg cccgttacaa
     2761 catgcaggac gtgggcggca ccgcgaaggc gatcgagcgc ggtgtcgcac tggtacgcga
     2821 gatgctgcct gctgccaacc gcgtcgagcg cacgccggta ccagcgagcc acctgatgct
     2881 cgggctacag tgcggagggt ccgacggcta ttccgggatc acggccaatc cggcgctcgg
     2941 caacgcggtc gaccggctgg tggcgcacgg tggcacggca atcctgtcgg atacgccgga
     3001 gatttatggc gccgagcacc tgctgacgcg gcgtgcggtg tcacgcgagg ttggagagaa
     3061 actgatcgcc cgcatcaagt ggtgggagga ttacaccgcg cgcaatcacg gcgaaatgaa
     3121 caacaaccca tcgcccggca acaaggccgg cggattgacg acaatcctcg agaagtctct
     3181 cggcgcggtc gcgaagggcg gcaccaccaa cctggtcgat gtctacgagt atgcgcagcc
     3241 ggtcgacaag cagggcttcg tctacatgga cacgccgggg tacgacccgg tgtccgcgac
     3301 aggacaggtc gccggcggcg ccaacatgat cgtcttcaca accggacgcg gctcggccta
     3361 tggctgcgcg ccgtagccgt cgctgaagct ctcgaccaac agcgaactgt ggcagcgcca
     3421 ggaagaggac atcgacctca attgtggcga aatcgcggac ggcgcggcaa ccatcgagca
     3481 ggtcggtgag cgcctaattc aactggtact cgccaccgcg tcgggctcaa gaaccaagag
     3541 tgaacgcttg ggctacggcc agagcgaatt cgttccatgg caactcggcg cgacgatgta
     3601 gcgccgcttg atgacgcaat acgcagttca accaggagga gacatgaagg acagcatctt
     3661 cgcgcagttt cgcattggtc acgcccgttg ggtcgtgggt gtcgcggcag ccctgcttgc
     3721 ggttggcggt gtatccgcgc agaccaagct caagttcgcg cacgtttacg aagtcagcga
     3781 gccgtatcac accgctgccg tctgggccgc gggggagatc ggcaagcgaa ccagcaatcg
     3841 ctacacgatg gaggtgtttc ctgcctcgac actcggcaag gagaccgaca tcaaccaggg
     3901 cctgacgctc ggcaccgttg acgtcatcta cacggggcag ttgttcgcgg ggcgcgcgta
     3961 cgggccgatt gcaatcggcg gcgcaccgta catgttccgc gacttcaatc actggaaggc
     4021 gttttccaca agtccgctgt tcggtgaact ggccgagggt tatcgcggca aaagcggcgg
     4081 caacaaggtt cttgctatca cctactacgg cgagcggcag gtcacgtcga acaaacccat
     4141 caacaagccc gaggacatga agggcctgaa gatccgcgtg ccggatgcgc cgctgtacac
     4201 gatgttcccg cgagcggtcg gtgccaatcc gacgccaatt gcgtttgccg aggtgtacct
     4261 cgcgctgcag aacggcacgg ttgatgcgca ggaaaatccg ctgccgacga tcgatgcgaa
     4321 gaagttctac gaagtgcaga agtacatcgt gctgaccggg catatcaccg atgcgttgct
     4381 gaccattgtc ggcggaccga cctggagcaa gctgtcggac gtcgacaaga agatcttcga
     4441 agaggtgttt cgtgaggcgg ctgtgcgcgc aacgagccag atcatcgaga tcgaagccaa
     4501 gctcggcgcc gagtttgcca agcgcggcaa ggaagtcgtc aaggtcgacc gcgcgccgtt
     4561 ccgcgaatcg gttgccaaga tgcacaacgg acccgacgcg acctgggaca aggccaccta
     4621 cgacaagctg caggcgttga aataagcggt gagatacaag ctgtgagatt ccggcggcgg
     4681 gcctccgtga cgacctgccg ccggacactc ccagtgcgct gaccgcgccg ccaggaatga
     4741 cctactgctg ggcgaagacg ggtgaacaca ccagcaggag ggcggacagc aggagggcgg
     4801 tcttcatggt cagctccggt ttgtgagggt cacggacggt agaagtacgg atcgagcacg
     4861 ctgacttcgg tgatgcggcc gatcggcagg ccgttgcgtt ccgccacctt gcggatcgtt
     4921 tccggggccg gcccgtcgta gatgcagaaa gtctgcttgc ggtccgtcgt cacgtatgag
     4981 tggacccagg tcacgccatg gtcggcgttg gtggcaacca ctccagcgac gactcggcga
     5041 ccttcatcgt tcatcgggat cgacaggccg tcaggaaagg ttcgttcgat gagatagcgg
     5101 ggcatcagga tatccgggag gttggaagca cgcactgcca tgaccgcact ttgaagatcc
     5161 ccgcgctgcc tggcgt
//
LOCUS       DATIRT010000269         4244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_531115, whole genome shotgun sequence.
ACCESSION   DATIRT010000269 DATIRT010000000
VERSION     DATIRT010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531115"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4244)
                     /locus_tag="VLN25_06145"
     CDS             complement(<1..>4244)
                     /locus_tag="VLN25_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HSQ10179.1"
                     /translation="DCDIHYETPHPSTFSFNSPLGACETCRGFGRVIGVDYGLVIPDE
                     RKTLRGGAIKPFQTKSFIESQRDLEKCAAKDGIPLDVPYRELTDVQKKWVIDGGTGWK
                     SWKKSWPGTWYGVKRYFEWLESKAYKMHIRVLLSRYRSYTPCPVCAGSRLRPDATLWR
                     VGANEEAQVAIPSGGRYARFRPVHASWSDQQLTSLPGLSLHDLMLLPIERVKRFFDAV
                     TLPPPLDEATEMLLDELRARLAFLVDVGVGYLTLDRQSRTLSGGEVQRINLTTALGTS
                     LVNTLFVLDEPSIGLHPRDMHRVVEVMHRLRSAGNSLVVVEHDPQVMLAADRVIDMGP
                     GPGERGGRIVFNGTPDELTHAETLTGRYLGGRQSVDATAPLPVPANAPRLILQGVREH
                     NLKSINVEIPLKRLVCVTGVSGSGKSTLIQDVLYPVLAKAKGRSTETPGAHDRLLGDD
                     WLDDVAFVDQSPIGKTTRSNPASYVGAFDAIRAIFAKSRLAKERNYTAGTFSFNSGDG
                     RCPTCGGNGFEHVEMQFLSDVYLRCADCDGKRFRPETLEVTVAKDGRSANIADVLAMT
                     VTEALAFFRDDAEVTRVLTPLVDVGLEYVKLGQPVPTLSGGEAQRLKLAGFLAEAARG
                     KPPTRGTLFMFDEPTTGLHFDDVAKLMRALRKLLAAGHSLVVIEHNLDVISAADWLID
                     LGPEGGDGGGELVGAGPPDALIAEPRSHTGRELKAYRDALLRHATRVNEPRAEYAVGR
                     PLQTLMSERRRRQRDVIEVRNAREHNLKSINVDIPRDTFTVITGVSGSGKSTLAFDIL
                     FNEGQRRYLESLNAYARSIVQPAGRPDVDAIYGIPPTVAIEQRTSRGGRKSTVATLTE
                     VYHFLRLIYVKLGVQHCPDCGVPVTPQSFDAIVAQVMRESKGQHIGVLAPLVVNRKGV
                     YTDMAKWAAARGHSHLRVDGVFLPTAKFPRIERFKEHTIELPVADLVVAPDNEARLRG
                     ALATALEHGHGVVTVISPLQSLQEAIARGHWGPTLGQKSFSTKRACPSCGTSFPEPDP
                     RLFSYNSKHGWCASCFGTGLKLAGFDAEQSGEEVWWNAWFEGEAAPCESCHGARLNRT
                     ALAVRFRGQSIAELTRGAVADARTFFDTLALNGREAEIARDALAEIRSRLSFLEEVGL
                     GYLALDRAAPTLSGGEAQRIRLAAQLGSNLQGVAYILDEPTIGLHPRDNRILLDALQK
                     LEAKGNTLVVVEHDEDTIRRAAHLIDIGPAAGVRGGRVVAQGSVDDLMAAPESATGAF
                     LRTPLAHPLHARRPVSRATPRLTVSDARMHNLRRVDAGFPLGRLTVVTGVSGSGKSTL
                     ARDVLLANLQGVVGQRKPKEQWSGCAAIEGWQALDRVLEVDQTPIGKTPRSCPATYVG
                     FWDAVRRLFAETLEARTRGYGASRFSFNTAGGRCDA"
BASE COUNT          680 a         1406 c         1437 g          721 t
ORIGIN      
        1 caggcatcgc aacggccgcc cgcagtgttg aacgaaaagc ggctggcacc gtagcctcgc
       61 gtgcgcgctt ccagcgtctc cgcgaacagc cggcgaacgg cgtcccagaa tccgacatac
      121 gtggccgggc acgagcgcgg cgtcttgccg atcggcgtct gatcgacttc gagcacgcgg
      181 tcgagcgcct gccagccttc gatcgccgcg cagcctgacc actgttcctt tggcttgcgt
      241 tgccccacga caccctgcag gttggcaagc agcacatctc gcgcgagcgt ggatttgccg
      301 ctgccggaca cgcccgtcac gaccgtcagg cggccgagcg gaaaaccggc atcgacgcgc
      361 cgcaggttat gcatgcgcgc gtcgctcacc gtgaggcggg gagtcgcacg tgatactggg
      421 cggcgagcgt gcaacggatg ggcgagcggc gtcctgagaa acgcaccggt agcggactcc
      481 ggtgctgcca tcaggtcatc gacgctgccc tgggcgacga cgcggcctcc gcggacgccg
      541 gcggccgggc cgatgtcgat caggtgcgcc gcgcgccgga tcgtgtcctc gtcatgctcg
      601 accaccacca gtgtgttgcc cttggcctcg agcttctgca gcgcgtcgag caggatccgg
      661 ttgtcacgcg gatgcaggcc gatcgtcggc tcgtcaagaa tgtaggcgac gccctgcagg
      721 ttggagccga gttgcgccgc cagccggatg cgctgcgctt cgccgcctga gagcgtgggc
      781 gccgcgcgat cgagcgccag gtagccgagt ccgacttctt ccaggaagga tagccgcgag
      841 cggatttcgg cgagtgcatc gcgtgcgatc tccgcctcgc gaccgttcag cgcgagcgtg
      901 tcgaagaatg tgcgggcatc ggcgaccgcg ccgcgggtga gttcggcgat cgactgtccg
      961 cggaaacgca cggccagcgc ggtccgattg aggcgcgcac cgtgacacga ttcgcaaggc
     1021 gccgcttcac cttcgaacca cgcgttccac cacacctcct cgccgctctg ctcggcgtcg
     1081 aagccggcga gcttgagtcc ggtcccgaag cacgacgcgc accagccgtg cttgctgttg
     1141 taggaaaaca gccgcggatc gggttcggga aagctcgtgc cgcacgacgg gcaggcgcgc
     1201 ttggtcgaga agctcttctg tccgagcgtg ggcccccagt ggccacgcgc gatggcctcc
     1261 tgcagcgatt gcagcggtga aatgacggtc actacgccgt ggccgtgctc gagcgccgtc
     1321 gccagcgcgc cccgcaaccg cgcttcgttg tccggcgcga cgaccaggtc ggccaccggc
     1381 aattcgatcg tgtgttcctt gaagcgttcg atgcgcggaa atttggcggt cggcaggaat
     1441 acgccgtcga cgcgcaggtg cgagtgcccg cgtgctgccg cccacttggc catgtcggtg
     1501 tagacgccct tgcgattgac gaccagcgga gcgagcacac cgatgtgctg tcccttcgac
     1561 tcgcgcatga cctgcgcgac gatggcgtcg aatgactgcg gcgtcaccgg cacaccgcaa
     1621 tccgggcagt gctgcacgcc gagctttacg tagatgagcc gcaggaagtg atagacctcg
     1681 gtgagcgtcg ccacggtcga cttgcggccg ccgcgcgacg tacgctgctc aatcgcgacc
     1741 gtcggcggaa tgccgtagat cgcgtcgacg tcgggacgcc cggccggctg cacgatcgag
     1801 cgcgcatagg cgttcagcga ttcgagatac cggcgctggc cttcgttgaa caggatgtcg
     1861 aaggccagcg tcgacttgcc ggaacccgac acgccggtga tcaccgtgaa cgtgtcgcgc
     1921 gggatgtcga cattgatcga cttgaggttg tgttcacgtg cattgcgcac ttcgatgacg
     1981 tcgcgctggc ggcgacgccg ttcgctcatc agcgtctgca gcggtcggcc gacggcgtac
     2041 tcggcgcgcg gctcattcac gcgcgtggcg tggcgaagca gcgcgtcgcg ataggccttc
     2101 agttcacggc cggtatgcga gcggggttcc gcaatcagcg catcgggtgg accggctccc
     2161 acaagttcgc cgccgccatc gccgccttcg gggccgaggt cgatcagcca gtcggccgcc
     2221 gagatcacgt cgagattgtg ttcgatgacg accagcgagt gaccagccgc cagcagcttg
     2281 cgcaaggcgc gcatcagctt ggcgacgtcg tcgaaatgca ggccggtggt cggttcatcg
     2341 aacatgaaca gcgtcccgcg cgttggcggc ttgccgcgtg ccgcctcggc caggaagccc
     2401 gcgagcttga gtcgctgcgc ctcgccgccg gatagcgtcg gcaccggctg gccgagcttc
     2461 acgtattcga ggccaacgtc gacgagcggt gtcagcacgc gcgtgacttc ggcgtcgtcg
     2521 cggaaaaacg ccagcgcctc cgtgaccgtc atggcgagca cgtcggcgat gttcgcgctg
     2581 cgcccgtctt tggccaccgt cacctcgagt gtctcgggcc ggaagcgttt cccgtcgcag
     2641 tcggcacagc gcaggtacac gtcggacagg aactgcatct cgacgtgctc gaagccgttg
     2701 ccgccgcagg tcgggcagcg cccgtcgccc gagttgaacg agaacgtgcc agcggtgtag
     2761 ttgcgctcct tcgcgaggcg ggacttcgcg aagatggcgc ggatggcatc gaaggcgccg
     2821 acgtagctgg ccgggttcga gcgcgtcgtc ttgccgatcg gcgactggtc gacgaaggcg
     2881 acgtcgtcga gccagtcgtc accgagcagc cggtcgtgag cgcccggagt ttctgtgctc
     2941 ctgcccttgg ctttcgccag cactggatac agcacgtcct ggatcagcgt cgatttgccg
     3001 ctgcccgaca cgccggtcac gcacacgagc cgcttcagcg gaatctcgac attgatggac
     3061 ttcaggttgt gctcgcgtac gccctgcagg atcaggcgcg gtgcattcgc gggcacgggc
     3121 agcggtgccg tcgcatcgac cgactgccgc ccgccgagat agcgcccggt cagcgtttcc
     3181 gcgtgtgtca gttcgtcggg agtgccgttg aagacgatgc ggcccccgcg ttctccgggc
     3241 ccgggcccca tgtcgatcac gcgatcggcg gcgagcatca cctgcggatc gtgctcgacc
     3301 accaccagtg aattgcccgc gctgcgcagc cggtgcatca cttcgaccac gcggtgcatg
     3361 tcgcgcgggt gcagtccgat cgacggctcg tcgagcacga acagcgtgtt gaccagcgag
     3421 gtgccgagcg ccgtggtcag gttgatgcgc tggacctcgc cacccgaaag cgtgcggctt
     3481 tgccggtcca gcgtcaggta gccgacgccg acatcgacca ggaatgccag ccgcgcccgc
     3541 agctcgtcga gcagcatttc ggtggcctcg tcgagcggtg gcggcagggt gactgcatcg
     3601 aaaaatctct tgacgcgctc aatcggcagc agcatcaggt cgtgcagtga gagacctggc
     3661 aaggacgtga gttgttgatc cgaccacgac gcatgcaccg gccgaaaccg cgcgtagcgt
     3721 ccgccggacg gaatcgccac ctgtgcttcc tcgttcgcgc cgacccgcca gagcgtcgca
     3781 tccggccgca ggcggctgcc cgcgcacacc gggcaggggg tgtacgaacg gtagcgcgag
     3841 agcagcacgc ggatgtgcat cttgtaggcc ttgctctcga gccactcgaa gtagcgcttg
     3901 acgccgtacc aggtgcccgg ccagctcttc ttccagctct tccagccggt gccgccgtcg
     3961 atgacccact tcttctggac gtcggtcagc tcgcggtacg gcacgtcgag cgggatgcca
     4021 tccttcgctg cgcacttttc gaggtcacgc tggctctcga tgaaggactt ggtctggaac
     4081 ggcttaatcg caccgcctcg cagcgtcttg cgctcgtcgg gaatcaccag cccgtagtcg
     4141 acaccgatca cgcggccgaa gccgcggcag gtttcgcagg cgccgagcgg ggagttgaac
     4201 gagaaagtcg acgggtgcgg cgtctcgtag tgaatgtcac agtc
//
LOCUS       DATIRT010000270         3069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1220345, whole genome shotgun sequence.
ACCESSION   DATIRT010000270 DATIRT010000000
VERSION     DATIRT010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220345"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VLN25_06150"
     CDS             <1..231
                     /locus_tag="VLN25_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HSQ10180.1"
                     /translation="TLGGYGDMPLMEDIDFSRRARRISWPGALQPAAVTSGRRWEQRG
                     VWRTILQMWSLRWRYYRGVDPAILARDYREVR"
     gene            231..854
                     /locus_tag="VLN25_06155"
     CDS             231..854
                     /locus_tag="VLN25_06155"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HSQ10181.1"
                     /translation="MSGLQVAVLARAAVPGAAKTRLIPRLGAERAAALQAHLTERALQ
                     RARASGADVVLWFDGVPDESTLELARACNADLHVQPVGDLGTRMHSALVYAQRCDRIG
                     IVIGTDCPAQQPDDILQAGMLLASHDVVLQPALDGGYVLIGMREPQRDLFSRINWGTD
                     TVLDTTRQRLLTLGLRYAELTPLPDLDRPDDLELALRNGWLDRGTWS"
     gene            851..1576
                     /locus_tag="VLN25_06160"
     CDS             851..1576
                     /locus_tag="VLN25_06160"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HSQ10182.1"
                     /translation="MMRVVFAVTIAASSAALAAPMPTPFSSAPDSRVPPPWQVITLPK
                     IPRHTQYEIVTLDGKRVVKAEADGSYANVVHPLHADVATTPVLRWSWRVDRFPAASDL
                     SLKAGDDVAAKVCVLFDLPLDRLGLIDRLKIELGRRLFKLDLPAATVCYVWERTLASG
                     TWLPNVYTDRVRMLVLRSAAAGEQGRWFDERRDLRADFARAFGTEAAGGLPPISAIAF
                     ASDADNTGSTALAYFGDILLVTE"
     gene            1573..2676
                     /locus_tag="VLN25_06165"
     CDS             1573..2676
                     /locus_tag="VLN25_06165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSQ10183.1"
                     /translation="MKRLLLVGGGHAHVLVLRELARRRPAGLEVMLVTPHDRQLYSGM
                     LPGWIAGHYTTDDLAIPLRPLLQAAGAGGLLDRIVSLDPKQRRVVTAGGTTLEYDLIS
                     LATGSDIAADAIEGARQWALPLRPIEEFVSKWTVLAPQLAEADRPKIVVIGAGAGGVE
                     VALAIAYALRAAGNDTQVQLVSGGNLLPGYGESTRQKIRSALVRANVRMLESTVKQVT
                     SGHVDLREGASLSSDLTLLASGASAPAWLRSTGLALDDAGFIQVDRRYRSVSHDTVFA
                     AGDVATMVGAPRARSGVYAVRAGPRLADNLIRQAQGQRLSTHTPQRTALYLLATGPQH
                     AIASWNGLAWNGDWVWRWKDRIDRGFIAQFRHA"
     gene            2684..>3069
                     /locus_tag="VLN25_06170"
     CDS             2684..>3069
                     /locus_tag="VLN25_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HSQ10184.1"
                     /translation="MRALVVLALLFVVVPLLLLAAGQLGLLTGKAPSGLGVKDGKLQP
                     PSRTPNSVSSQAMLWPEGDYASVYAQIEPLRLSGDGTVAMTRLRALLAAWPGARIVKE
                     RPDYLRAEFSTRWFRFVDDVEFWLDPA"
BASE COUNT          475 a         1017 c         1037 g          540 t
ORIGIN      
        1 acactcgggg gctatggcga catgccgttg atggaagaca tcgacttctc gcggcgcgcg
       61 cggcggatct cgtggccggg cgcgctgcag ccggccgcgg tcacctcggg acgccgctgg
      121 gagcaacgcg gcgtctggcg caccatcctg cagatgtggt cgctgcggtg gcgctactac
      181 cgcggcgtcg acccagccat cctggcgcgc gactatcgcg aggtccgctg atgtcagggc
      241 tgcaggtcgc ggtgctcgca cgggctgcgg tgccaggggc ggccaagacg cgcctgattc
      301 cgcggctcgg agccgagcgt gctgcggcac tgcaggccca cctgacggaa cgcgcactgc
      361 aacgagcacg cgcttccggt gccgacgtcg tgctgtggtt cgacggcgtg cccgatgagt
      421 cgacgctgga acttgcgcgt gcatgcaatg cggatctgca tgtacagccg gttggcgacc
      481 ttggaacgcg catgcacagc gccctggtct atgcgcagcg gtgcgatcgg atcgggatcg
      541 tcatcggcac ggactgtccc gcacaacagc ccgatgacat cctccaggca ggtatgctgc
      601 ttgccagcca tgacgttgta cttcaacccg ctcttgacgg cggctacgtg ctgatcggca
      661 tgcgtgagcc acagcgcgat ctcttctccc ggatcaactg gggcacggac acagtgctcg
      721 acaccacacg ccagcgcctt ctaaccctcg gactccgcta cgcggagctg actccattgc
      781 ccgatctcga tcggccggac gacctggaac ttgcactgcg aaacggctgg ctcgatcgcg
      841 ggacctggtc atgatgcggg tcgtgttcgc cgtcacgatc gcagcgtcca gcgcagcgtt
      901 ggccgccccg atgcccacgc cgttttcgtc tgcgccggac tcgcgcgttc cgcccccgtg
      961 gcaggtgatc acgctgccga agattccgcg gcacacgcaa tacgagatcg tcactttgga
     1021 cggcaagcgc gtcgtcaaag ccgaggccga tggctcctac gccaacgtcg tgcacccgtt
     1081 gcatgccgac gtggcaacca caccggtcct gcgatggagc tggcgcgtcg atcgcttccc
     1141 ggcagcaagc gatctgtcgc tcaaggccgg tgacgacgtg gccgccaagg tctgcgtgct
     1201 cttcgatctt ccgctggatc gccttggtct gatcgatcgg ctgaagattg aactcggtcg
     1261 gcggctgttc aagctggatt tgccggccgc cacggtgtgc tacgtgtggg agcgcacgct
     1321 ggcctccggt acgtggctgc ccaacgtgta cacggaccgc gtgcgcatgc tggtgctccg
     1381 ctccgcggca gccggcgagc agggacgctg gttcgatgaa cgacgcgacc tgcgcgccga
     1441 cttcgcgcgc gcgttcggta ccgaggcggc cggtggtttg ccgcccatca gtgcgatcgc
     1501 cttcgccagc gacgccgaca acacgggcag caccgcgctc gcctactttg gcgacatcct
     1561 gctggtgact gaatgaagcg attgcttctt gtgggcgggg gacacgcgca tgtcctcgta
     1621 ctgcgcgaac tcgcacggcg ccggccggcc ggcctcgaag tcatgctcgt cactccccat
     1681 gaccggcagc tgtacagcgg catgctgcct ggctggatcg cggggcacta cacgaccgac
     1741 gatctcgcca ttccgctgcg tcccttgctg caggcggcgg gcgcgggggg gctgctcgat
     1801 cgcatcgtca gcctcgatcc taagcaacgg cgcgtggtca ccgcgggggg cacgaccctc
     1861 gaatacgacc tgatctcgct ggccaccggc tcggacattg ccgccgacgc gatcgagggc
     1921 gctcgtcagt gggcgctgcc actacggccg atcgaggaat tcgtgtcgaa gtggacagtg
     1981 ctcgcgccac aattggcgga agcggatcga cccaagatcg tcgtgatcgg cgccggcgcc
     2041 ggcggtgtcg aggtggcgct ggccatcgcc tacgctttgc gcgccgcggg aaacgacacc
     2101 caggtccagc tcgtcagcgg cgggaacctt ttgccgggat atggcgaatc tacgcggcaa
     2161 aagattcgtt cggcgctggt tcgagcgaac gtgcgtatgc tcgaatcgac cgtcaagcaa
     2221 gtcaccagtg gtcatgtcga cttgcgggag ggagcctcgc tgagtagcga cctgactttg
     2281 ctggccagcg gcgccagcgc gccggcctgg ctgcgcagta ccggactggc gctggacgat
     2341 gcaggcttca tccaggtcga tcgccggtat cgatcggtgt cgcatgacac ggtcttcgcc
     2401 gccggcgatg tcgcaacgat ggtcggtgcg ccacgtgcgc gctctggggt ctacgccgta
     2461 cgcgcgggac cgcggctcgc cgacaacctc attcgacagg cgcagggtca gcggctgagc
     2521 acacatacgc cgcagcggac cgcgctctac ctgttggcga ccggaccgca acacgcgatc
     2581 gcttcgtgga acggcctcgc atggaacggc gactgggtgt ggcgatggaa ggatcgcatc
     2641 gatcgtggtt tcatcgcgca gttccgccat gcctgaggtg atcatgcgag cgctcgtcgt
     2701 cctggcattg ctgttcgtgg tcgtgccgct gctgctgctg gcggcaggcc agctgggtct
     2761 gctcaccggc aaggcgccct cgggcctggg cgtgaaggac ggcaagctcc agccgccctc
     2821 gcggacgccc aacagtgtca gcagccaggc aatgctctgg ccggagggcg actacgccag
     2881 cgtctatgcg cagatcgaac ccttgcggct cagcggcgac ggcaccgtcg cgatgacgcg
     2941 gctgcgcgcg ctgctcgccg cctggcccgg tgcacgcatc gtgaaggaac gaccggacta
     3001 tctgcgggcc gagttcagca cccgctggtt ccgtttcgtc gatgacgtgg agttctggct
     3061 tgacccggc
//
LOCUS       DATIRT010000271         8913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1417361, whole genome shotgun sequence.
ACCESSION   DATIRT010000271 DATIRT010000000
VERSION     DATIRT010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8913
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1417361"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..163
                     /locus_tag="VLN25_06175"
     CDS             <1..163
                     /locus_tag="VLN25_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008641741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSQ10185.1"
                     /translation="VPKIGGQSPQYIEAALKAYRQGDRTHPTMVGVAKGLSDQQIAAL
                     AAYYAARGK"
     gene            182..505
                     /locus_tag="VLN25_06180"
     CDS             182..505
                     /locus_tag="VLN25_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSQ10186.1"
                     /translation="MRILVAASVALVAVSAQAADVAKGEQLAQAQCAACHGKDFASPV
                     DPSYPKLAGQYPYYLEKALRDYQTGARRNAIMAGLAKPLTRADIQNVSDYLSRLPGPL
                     GNEKR"
     gene            complement(544..987)
                     /locus_tag="VLN25_06185"
     CDS             complement(544..987)
                     /locus_tag="VLN25_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004553866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HSQ10187.1"
                     /translation="MFAPSKDDVRRFFCETWSKHRNGQILTPIETMALDWIVEHPEYH
                     DVLSSEEAAATEYRVDEGRTNPFLHLSMHLAIAEQLSIDQPPGIRAAYQRLVARRSDA
                     HAAAHDVMECLGEVIWSAQRSGDALPPDEMSARYLECLSRRATQV"
     gene            complement(990..1667)
                     /gene="nth"
                     /locus_tag="VLN25_06190"
     CDS             complement(990..1667)
                     /gene="nth"
                     /locus_tag="VLN25_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HSQ10188.1"
                     /translation="MNPAKRRAIYEQLRSLNPHPATELEFATPFQLLVAVVLSAQATD
                     KSVNLATRGLFKAAPTPKAIAGLGEERLMEYIKTIGLYRMKAKNVIGLCRQLLELHGG
                     EVPRDRAALESLPGVGRKTANVVLNTAFGEPTIAVDTHIFRVANRTGMAPGADVDAVE
                     QRLEKFTPEEFRRDAHHWLILHGRYVCKARTPECWRCPIAEWCEFRPKSPAPRAPRSG
                     FPLRAES"
     gene            complement(1664..2326)
                     /gene="rsxB"
                     /locus_tag="VLN25_06195"
     CDS             complement(1664..2326)
                     /gene="rsxB"
                     /locus_tag="VLN25_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HSQ10189.1"
                     /translation="MSDPTAMLADRIDALLPQTQCTKCGHDGCRPYAEAIAAVRADIN
                     QCPPGGAVGIVRLSQLTGRPVVPLDPNNGIEQPLRVAVIDEQLCIGCTLCIQACPVDA
                     IVGAARRMHSVIPEWCNGCDLCITPCPVDCITMVPVRPERTWSNDDARQSRARYEARR
                     RRLAGERREREQQLLQKAITRLDELGRRDDLSQDTVGHKQATVRAAIERARVRRQAEM
                     KR"
     gene            complement(2393..3625)
                     /gene="phaZ"
                     /locus_tag="VLN25_06200"
     CDS             complement(2393..3625)
                     /gene="phaZ"
                     /locus_tag="VLN25_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HSQ10190.1"
                     /translation="MLYHLHELQRRFLNPLSVWAQATSELFSSPYSPLAYSPMSRRLS
                     AGYDLLHRIGKQYEKPEFGLKTTRIGDAEVPVVEEITIEKPFCRLLHFKRLVPAKLKR
                     PADPRVLIVAPLSGHHATLLRDTVRALLPDHDIWITDWIDARLVPITAGPFHLDDYVD
                     YVIDFVRELGPDVHLISVCQPTVPVLAAVSLMASSGDAKLPKTMTMMGGPIDSRKSPT
                     QVNALAIRKNFEWFENTVIFKVPSAYPGYLRKVYPGFLQHAGFVAMNPDRHLNSHWDY
                     YLDLVKGDMDDVEGHRGFYDEYNAVIDLPAEYYLDTIRIVFQKHSLPNGTWKVHGQLV
                     KPEDITSVALFTIEGELDDISGSGQTQAAHGLCKGIPKAKKRHLTAEGAGHYGIFSGR
                     RWREQIAPKIKSFIREHA"
     gene            complement(3809..4132)
                     /gene="fdxA"
                     /locus_tag="VLN25_06205"
     CDS             complement(3809..4132)
                     /gene="fdxA"
                     /locus_tag="VLN25_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HSQ10191.1"
                     /translation="MTHVVTESCIKCKYTDCVDVCPVDCFREGPNFLSIDPDECIDCA
                     VCIPECPVNAIYAEEDVPEDQRDFIKLNVDVSKAWPSITRMKPHPADADEWKDVREKR
                     HLLER"
     gene            4289..5473
                     /locus_tag="VLN25_06210"
     CDS             4289..5473
                     /locus_tag="VLN25_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSQ10192.1"
                     /translation="MSTAALVAAPLRAQTAATIRIGEINSYSTIPQFTVPYRMGWQLA
                     VEEANAGGGLLGRKVEVISRDDAGKPDDAIRLATELVSNGKVALLAGTFLSNVGLAVA
                     EFALRNKVLFVAAEPLTDAMVWEKGNRYTFRLRPSTHMQSAMLVEEAARLPAKRWAIV
                     APNYEYGQSAVASFKALLKARRPEVEFVAEQWPALGKLEAAGTVQALIAARPEAIFNV
                     TFGADLPRLVREGNLRGLFPRVPVVSLLSGEPEYLDVLKDETPRGWIVTGYPWDQIAT
                     PEHAKFFNAYFKRYNDTPRLGSVVGYATFTAIFEAVRKAKSTDTEKLIASLRGLTWTT
                     PFGPATFRALDHQSTMGAYVGKLDLRDGRGTMVNWRYADGRNYLPTDAEVKARRPAEA
                     MK"
     gene            5530..7362
                     /locus_tag="VLN25_06215"
     CDS             5530..7362
                     /locus_tag="VLN25_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSQ10193.1"
                     /translation="MDLVFGQLLAGISYGSTLFLVSAGLTLIFGVTRVVNFAHGALYM
                     LGAYLAVTLTAALPATPVGFFGGAVLAAMIVALAGVLVEFLVLRRIYAAPEIFQLLAT
                     FGVALIIQDLVLAAWGPEELFAPRAPGLKGAIEIGGAFIPEYNLLLTLVGPVIFGALW
                     WIFRRTRWGTIVRAATQDREMAGALGVNERRLFSTVFALGAGLAGLAGALQIPRETVN
                     LQMDLTILVEAFVVVVVGGLGSITGAFWASMAIGVLHAFGIWLLPQSTLVLVFVVMAV
                     TLIVRPYGLFGRPDVASRTVRTATHPPFTLPSPRGRMGVAAAIVVFAALPMFAGDYAL
                     VIATEMAILALFAASLQFFMGHGGLVSFGHAAFFGLGAYAAAMLVKNFAAPFVAALLA
                     APVVAGLAAAIAAFFVLRAQGVYASMLTLAFAQILWSIAVQWVDVTGGDNGLLGIWPP
                     TTSRIVYYAITLGVVAVSLIVLRRAALAPFGFALRATRDAPERAEALGIDVRRVRWAA
                     FVAGGVFGGLAGALQAFQKGAVFPNALSIPLSVDALVMVLLGGLQTISGPVVGAIAYH
                     GLSTELIRHAEYWRLALGVTIVLLVVLFPQGIVGFLRARWARTP"
     gene            7359..8102
                     /locus_tag="VLN25_06220"
     CDS             7359..8102
                     /locus_tag="VLN25_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSQ10194.1"
                     /translation="MSELRVTHLKRSFGGVQAVDDVSFALAAGSVTALIGPNGAGKST
                     LFNLIDGQLGPDAGTIEFEGKSLVGVPTSARTRAGIGRTFQVAQTFGSMTVSQNAQLA
                     LAAAAGLALSAVTPLERHAPEGAAHLLQQVGLNARGDAPAAALAYGDLKRLELALALA
                     ANPRLLLMDEPTAGMAASERSSLMTLVVALANERRMTVLFTEHSMDVVFGFAQRVLVL
                     SRGRLIADGVPDVVRADRAVQAVYLGSEP"
     gene            8099..8797
                     /locus_tag="VLN25_06225"
     CDS             8099..8797
                     /locus_tag="VLN25_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSQ10195.1"
                     /translation="MTLLELRGVDAWYGAAQVVRGATLSVAAGEVVALIGRNGAGKTS
                     LLRAIMGLMPRSAGDVLLDGTSIARLAPFARARRGLGYVPEDRRIFTDLTVRENLELV
                     GRPHAGGFDTAALLSIFPNLAEMLERPASQMSGGEQQMLAVARTLAANPRIVLLDEPS
                     EGIAPLVVARMRDAVQAMRARGVATLLSEQNLAFVSGLADRAVLIEQGHVVGTADRDE
                     LSSSAAVRKVLGIG"
BASE COUNT         1387 a         2843 c         3015 g         1668 t
ORIGIN      
        1 cgtgccgaag atcggcgggc aatcgcccca gtacatcgag gctgcgctca aggcctatcg
       61 ccagggcgat cgcacgcatc cgaccatggt gggcgtcgcc aagggcctca gtgaccagca
      121 gatcgccgcc ctggccgcgt attacgcggc gcgtggcaag taagcgggca gcatgatgac
      181 gatgagaatt ctcgttgcgg ccagcgtcgc actcgttgca gtttcggcgc aggccgctga
      241 tgttgccaag ggcgaacaac tcgcgcaggc gcaatgcgcc gcgtgccatg gcaaggactt
      301 tgcctcgccc gtcgatccga gctatccaaa actcgctgga cagtatccgt actatctcga
      361 gaaggcgctg cgcgactacc agaccggcgc gcgcaggaac gctatcatgg cggggctggc
      421 gaagccgctg acgcgtgccg atatccagaa cgtgtcggac tacctgagcc gacttccggg
      481 gccgctcggc aacgagaagc gctagtcgcg ggccggcacc cagcggggtc caccgcacga
      541 aagtcagacc tgcgtcgccc gtcgtgacag gcactcgagg tagcgtgcgc tcatctcgtc
      601 ggggggcagc gcgtcgcccg atctctgtgc cgaccagatg acctcgccca ggcactccat
      661 gacgtcgtgc gcagccgcat gcgcatcgct gcgacgggcg accagccgct gatacgccgc
      721 acgaatgccc ggtggctggt cgatcgacag ctgctcggca atcgccaggt gcatcgacag
      781 gtgcaggaac ggattggtac gcccttcgtc gacgcgatac tccgtggccg ccgcctcctc
      841 gctcgaaagt acgtcgtggt actcgggatg ctcgacgatc cagtcgagcg ccatcgtttc
      901 gatcggagtc aggatctgcc cattccggtg cttgctccag gtctcgcaga agaagcgcct
      961 gacatcgtcc ttcgatgggg cgaacacgtt cacgactccg cccgcagcgg gaagccgctg
     1021 cgcggtgctc gtggcgccgg cgacttgggc cggaattcgc accactcggc aatcgggcag
     1081 cgccagcact ccggtgtccg tgccttgcag acatagcggc catgcagtat cagccaatgg
     1141 tgcgcgtccc tgcggaactc ctcgggcgtg aacttctcaa gtcgttgttc gacggcatcg
     1201 acatcggcgc ccggcgccat gccggtgcga ttcgcgacac ggaagatatg agtgtccacg
     1261 gcgatcgtcg gttcgccaaa tgcggtgttg agcacgacgt tcgcagtctt gcggcccacg
     1321 cccggaagcg actccaacgc ggcccggtcg cgcggcacct cgccgccgtg cagctccagc
     1381 agttgtcgac acagcccgat gacgttcttc gccttcattc ggtacaggcc aatggtcttg
     1441 atgtactcca tcagccgctc ctcgcccagc ccggcgatgg ccttgggcgt gggggcggct
     1501 ttgaacagtc cgcgcgtcgc cagattcacg ctcttgtccg tggcctgggc cgacagcacg
     1561 accgcaacaa gcagctggaa tggcgtcgcg aactcgagct cggttgccgg atggggattc
     1621 aggcttcgca gctgctcgta gattgcccgt cgcttcgcgg gattcatcgt ttcatttccg
     1681 cctgtcgccg tacgcgcgcg cgttcgatcg cggcacgaac ggtggcctgc ttgtgcccga
     1741 ctgtgtcctg cgacagatca tcgcgccgcc caagctcgtc gagtctggtg atggccttct
     1801 gcaacagctg ctgttcgcgc tcccggcgct cgccggcaag ccgccgccga cgcgcttcgt
     1861 agcgtgctcg tgactgccgt gcatcgtcgt tgctccaggt gcgctccggc cgtaccggga
     1921 ccatcgtgat gcagtccacc gggcacggcg tgatgcacag atcgcaacca ttgcaccact
     1981 cggggatcac gctatgcatg cgccgtgcag cgccgacgat cgcgtccacc gggcacgcct
     2041 ggatgcacag ggtgcagccg atgcagagct gctcgtcgat gacggcgacg cgcaggggct
     2101 gctcgatccc attgttcgga tcgagcggca cgaccggccg ccccgtcagt tgcgacagcc
     2161 ggacgatgcc aacggcgccg cccggagggc actgattgat atccgcacgc accgcggcga
     2221 tggcttcggc gtacggacgg cagccgtcgt gaccgcactt cgtgcactgg gtctgcggca
     2281 acaacgcatc gatgcggtcg gccagcatgg cggtgggatc ggacatcggc ggtcctgaag
     2341 gcgacgcgcg ccgagatcgc ggattatcgc tggctacggg gtggacccgc gctcaggcgt
     2401 gttcgcggat gaaactcttg atcttcggtg cgatctgctc gcgccagcgg cgcccgctga
     2461 agatgccgta gtgcccggcg ccttcggcgg tcagatgtcg cttctttgct ttcggaatgc
     2521 ccttgcacag gccgtgggca gcctgcgtct ggccgctgcc ggagatgtcg tcgagttccc
     2581 cctcgatcgt gaacagcgcc accgacgtga tgtcctccgg cttcaccaac tggccgtgaa
     2641 ccttccatgt gccgttgggc aggctgtgct tctggaacac gatcctgatg gtgtccaggt
     2701 aatactcggc cggaagatcg atcaccgcgt tgtactcgtc gtagaaaccg cgatgcccct
     2761 cgacgtcgtc catgtcgccc ttgaccaggt cgaggtaata gtcccaatgc gaattgagat
     2821 ggcggtccgg gttcatcgcg acaaatccgg cgtgctgcag gaatcccggg tagaccttgc
     2881 ggaggtagcc ggggtacgcc gacggcacct tgaagatcac ggtgttctcg aaccattcaa
     2941 agttcttgcg tatcgcgagc gcattgacct gagtcgggct ctttcggctg tcgattgggc
     3001 cgcccatcat cgtcatcgtc ttcggaagct tcgcatcgcc gctgctggcc atcagtgata
     3061 ctgccgccag caccggcacg gtcggctggc acacggagat caggtggaca tccgggccga
     3121 gttcacgcac gaaatcgatc acatagtcga catagtcatc gaggtggaac ggtccggccg
     3181 tgatcgggac cagacgggca tcgatccagt cggtgatcca gatgtcgtgg tcgggcagca
     3241 gtgcacgcac cgtgtcgcgc agcagggtgg cgtggtgccc cgacagcggc gccacgatca
     3301 gcacacgcgg atccgcgggg cgcttgagtt tcgccgggac aagacgcttg aaatgcagca
     3361 gccggcagaa cggcttttcg atcgtgattt cttcgaccac cggcacctcg gcgtcgccga
     3421 tgcgcgtggt cttcagtccg aactcgggct tctcgtattg tttgccgatc cggtgcagca
     3481 ggtcgtagcc cgccgacagc cgccgggaca tcggcgagta agcgagggga ctgtaggggc
     3541 tggagaagag ttcggaagtg gcctgcgccc acaccgaaag cgggttcagg aaacggcgtt
     3601 gcagttcatg caggtgatat agcacgccag gctccgctca acgctccgct cttcaccggt
     3661 tacgtcctcc ctacaggcga ccggatagta aaccaaattg gtcaggccgc ttttgatgtt
     3721 gatagggtgg gtcgacaggg caattcggcc ctctggacag ggccggcggt gggacttcca
     3781 accgagccgc gtccgggtcg aagcgggatc agcgttcgag cagatggcgc ttttcgcgca
     3841 cgtctttcca ctcgtcggcg tcagcggggt gcggcttcat gcgcgtgatg gacggccacg
     3901 ccttcgacac atcgacgttc agcttgatga agtcgcgctg atcctcggga acgtcttcct
     3961 cggcatagat cgcgttcacc gggcattccg ggatgcagac ggcacagtcg atgcactcgt
     4021 ccggatcaat cgacaggaag ttcggtcctt cgcggaagca gtcgaccggg cacacgtcga
     4081 cgcaatcggt gtacttgcac ttgatgcacg actcggtgac gacgtgcgtc atcgactgac
     4141 tcctgtccgt tcggtgaact gcgccgcaag actgaaaagt gcgccgcttt caaagccggt
     4201 attctagctg accagagccg aggacccggc tgttttccct ctgccgagaa gggccagtgg
     4261 catgaccacc cgacgcgaac tcctgctgat gtcgacggct gcgctcgtcg ctgcgccgtt
     4321 gcgcgcgcag accgcggcga cgatccgtat cggcgaaatc aacagctatt cgaccattcc
     4381 gcagttcacg gtgccgtatc gcatggggtg gcaactggcc gtcgaggaag cgaacgccgg
     4441 cggcggtctg ctcgggcgca aggtcgaagt gatctcacgc gacgatgccg gcaagccgga
     4501 cgatgcgatc cggctggcga ccgaactggt atcgaacggg aaggtggcgt tgctggcggg
     4561 aaccttcctc tcgaacgtcg gactggccgt cgccgagttc gcgttgcgca acaaggttct
     4621 tttcgtggcc gccgagccgt tgaccgacgc gatggtctgg gagaagggca accgctacac
     4681 ctttcgcctg cggccaagca cgcacatgca gtcggcgatg ctggtcgaag aagccgcgcg
     4741 gctgccggct aaacgatggg cgatcgtggc gcctaactac gagtatggcc agtcggccgt
     4801 ggcgagcttc aaggcactac tgaaggctcg gcgtccggag gtggagttcg tcgctgagca
     4861 atggccggcg ctgggtaagc tcgaggccgc cggcacggtg caggcgctga tcgcggcgcg
     4921 tcccgaagcg atcttcaacg tgacgttcgg cgccgatttg ccgcggctgg tgcgcgaggg
     4981 caacctgcgc gggctctttc cgcgcgtgcc ggtcgtgagt ctgctgtccg gtgaacccga
     5041 gtacctcgac gtgttgaagg acgagacgcc ccgcggctgg atcgtgaccg gctatccgtg
     5101 ggaccagatc gccacgccgg aacacgcgaa attctttaac gcctatttca agcgctacaa
     5161 cgacacaccg aggctgggtt cggtcgtcgg gtacgcgacg ttcaccgcga tcttcgaggc
     5221 agtgcggaag gccaagtcga ccgacacgga aaagctgatc gcctctctgc gaggcctgac
     5281 gtggaccacg ccgttcggcc ccgcgacgtt ccgtgcactg gaccaccagt cgacgatggg
     5341 tgcctacgtc ggcaagctcg accttcgcga cggccgcggc acgatggtca actggcgcta
     5401 tgccgatggc cgtaactacc tgccgacgga tgccgaggtc aaggcgcggc gcccggccga
     5461 ggcgatgaaa tagccccggt gcactgcgcc tgcgcgcggc cgttgcgccg ggcgatctga
     5521 gggcctgcca tggacctcgt gttcggtcag ttgctcgctg gaatctccta cggctcgacg
     5581 ctgttcctcg tcagcgccgg gctcacgctg atcttcggtg tcacgcgtgt cgtgaacttt
     5641 gcccacggcg cgctctacat gctgggggcg tacctggcgg tcacgctgac cgcggcgctg
     5701 cccgccacgc cggtcggttt cttcggtggc gcggtgctcg cggccatgat cgtggccttg
     5761 gcgggtgtac tggtcgagtt tctcgtgttg aggcgcatct acgccgcgcc cgagatcttt
     5821 cagctcctcg caacctttgg cgtcgcgctg atcatccagg atctcgtact ggccgcgtgg
     5881 gggccggagg aattgttcgc tccgcgcgcg ccgggcctga agggcgccat cgagatcggc
     5941 ggtgcgttca tccctgagta caacctgctg ctgacactgg tcggccctgt gatcttcggc
     6001 gcgctgtggt ggatcttccg ccggacgcgc tgggggacga tcgtgcgtgc cgccacgcag
     6061 gaccgcgaga tggccggtgc cctcggcgtc aacgagcgac ggctcttcag cacggtgttc
     6121 gcgctcggcg cgggcctggc cggcctggcg ggtgcattgc agatcccgcg cgagaccgtc
     6181 aatctgcaga tggacctgac gatcctggtc gaagcctttg ttgtcgtggt ggttggcgga
     6241 ctcggttcga tcaccggcgc gttctgggcc tcgatggcga tcggggtgct gcacgccttc
     6301 ggcatctggc tgctgccgca atccacgctc gtgctggtgt tcgtcgtgat ggcggtcacg
     6361 ctgatcgtgc gcccctatgg tctgttcggg cgccccgacg ttgcgtcgcg cacggtacgc
     6421 acggcaacgc atccaccatt cacgttgcca tcgccccgag ggcgcatggg ggtggccgcg
     6481 gcgatcgtgg tgtttgcggc cctgccgatg ttcgcgggcg actacgcgct cgtgatcgcc
     6541 accgagatgg cgatcctggc gctctttgcg gccagcctgc agttcttcat gggtcacggt
     6601 gggctggtgt cgttcggcca tgcggcattc ttcggtctcg gtgcctatgc cgccgcaatg
     6661 ctggtgaaaa actttgctgc acccttcgtg gccgcgctgc tggcggcacc ggtggtggcc
     6721 gggctcgcgg cggcgatagc cgcattcttt gtgctgcgcg ctcagggtgt gtatgcgtcg
     6781 atgctcacgc tggcattcgc gcagatcctc tggtcgatcg ccgtgcagtg ggtcgatgtg
     6841 accggcggcg acaacggcct gctcggcatc tggccaccga cgacgtcacg catcgtctat
     6901 tacgcaatca cgctgggggt tgttgcggtg tctctcatcg tgctgcggcg ggccgcgctg
     6961 gcgccattcg gtttcgcgct gcgtgcgacg cgcgatgcgc ccgagcgggc cgaggcgctc
     7021 ggcatcgacg tgcgccgggt gcgttgggcc gctttcgtcg cgggcggtgt ctttggtggt
     7081 ctggcgggcg cactgcaggc gttccagaag ggcgccgtgt ttccgaacgc cctgtcgatt
     7141 ccgttgtcgg tcgatgcgct cgtcatggtg ctgctcggcg gactgcagac gatttccggt
     7201 cccgtcgtcg gcgccattgc gtaccacggg ctgtcgacag agcttatccg gcatgccgag
     7261 tactggcgct tggccctcgg cgtgacgatt gtgctgctcg tggtgctctt tccgcagggc
     7321 atcgtcggtt tcctgcgggc tcgctgggcg aggacgccat gagcgagctg agggtcacgc
     7381 acctgaaacg ctcgttcggt ggcgtgcagg ccgtcgacga tgtgtcgttc gcactcgcag
     7441 cgggcagcgt gaccgccctg atcggcccca acggcgcggg caagtcgaca ctgttcaatc
     7501 taatcgacgg ccagctgggt ccggacgccg gcacgatcga gttcgaagga aagtcgctcg
     7561 tgggcgtgcc gacgtccgcg cgcacgcgcg ccgggatcgg gcgtacgttc caggtcgcgc
     7621 agaccttcgg ttcgatgacc gtcagccaga acgcgcaact cgcactggcc gccgcggcag
     7681 gccttgcgct gtcagcggtc acaccgctcg aaaggcatgc gccggaaggg gcagcgcacc
     7741 tgctgcaaca ggtcggcctg aatgcgcgcg gcgacgcgcc ggcggcggca ctggcgtacg
     7801 gcgacctcaa gcggctcgag ctggcactgg ctctggcggc gaatccccgc ctgctgctga
     7861 tggacgaacc gaccgcgggc atggcggcgt ccgaacggtc gtcgctgatg acgctcgtgg
     7921 tcgcgctggc gaacgagcgc cgcatgactg tcctgttcac cgagcacagc atggatgtcg
     7981 tcttcggctt cgctcagcgc gtgctggtgc tgtcgcgcgg gcggctgatc gccgacggtg
     8041 ttcctgacgt ggtgcgcgcc gaccgggcgg tccaggccgt ctacctgggc agcgagccat
     8101 gacgctgctc gaactgcgcg gcgtcgacgc atggtacggc gcggcacagg tggtgcgggg
     8161 agccacgctg tccgtggccg ccggcgaggt cgtcgcgctg atcggacgca atggtgctgg
     8221 caaaacgtca ctgctgcgcg cgatcatggg cctgatgccg cgcagcgccg gcgatgtgct
     8281 gctggacggc acatcgatcg cgcggctcgc gcctttcgcc cgagcacggc gcggcttggg
     8341 ctacgtgccg gaagatcgtc gcattttcac cgacctcacg gtgcgcgaga acctcgaact
     8401 ggtcgggcgg ccgcatgcgg gcgggttcga cacggccgcg ctgctgagca tcttccctaa
     8461 tttggccgag atgctggagc ggcccgcaag ccagatgagc ggaggcgagc agcagatgct
     8521 cgcggtggcg cgcacgctgg ccgccaatcc gcggatcgtg cttctcgacg agccgtccga
     8581 agggatcgcg ccgcttgtgg tcgcgcgcat gagggacgcg gtgcaggcga tgcgcgcacg
     8641 cggtgtcgcg acactgctgt cggagcagaa cctcgcgttc gtgtccggac tggccgaccg
     8701 ggccgtgctg atcgagcagg gccatgtggt cggcaccgcc gatcgcgacg agctttcgtc
     8761 gagcgccgct gtccgcaagg tgctgggaat cggatagcgc ggcggcacgg cgcgacccta
     8821 caatcgacgc atgatcatcc agtcgctgct cgataccgac ctgtacaagt tcacgatgat
     8881 gcaggtcgtg ctgcaccatt ttccgggtgc gca
//
LOCUS       DATIRT010000272         2450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1122813, whole genome shotgun sequence.
ACCESSION   DATIRT010000272 DATIRT010000000
VERSION     DATIRT010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122813"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..1407
                     /gene="murF"
                     /locus_tag="VLN25_06230"
     CDS             13..1407
                     /gene="murF"
                     /locus_tag="VLN25_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HSQ10196.1"
                     /translation="MSALMTVAELARTVAAQDVRGDGRIGFASVSTDTRTLPNGALFV
                     ALRGERFDGHDYAQSAADRGAAALLVEHAVEAAVPQIIVADTKHVLGLGAAQWRARFA
                     LPVIAVTGSNGKTTVTQMIGAILAQAHGERHHLATLGNLNNDIGVPLMLWQLGKQHKA
                     AVFELGMNHPGEIAYLAELVRPTVALVNNAQREHQEFMQSVEATAHENGEVLAALPLA
                     DMGVAVFPGDDPCTPIWRSLAGTRRVVDFALEAYAVVTATYELDAEGAHVSMATPAGL
                     VEARLAVGGVHNVRNALAAAACAMAVGIEPRDISAGLAAFRPVNGRGARLRTSSGAWL
                     IDDSYNANPDSVRAAIDLLAGCASPRVLVLGDMGEVGDRGPEFHREVGIYARERAIDV
                     LFALGTQTRETVAAFGPGGMHFDDVDTLMAALPAKVTAGSTVLVKGSRFMRMERVVQA
                     LAEPSAHGAAAGAH"
     gene            1407..2450
                     /gene="mraY"
                     /locus_tag="VLN25_06235"
                     /pseudo
     CDS             1407..2450
                     /gene="mraY"
                     /locus_tag="VLN25_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872184.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
BASE COUNT          371 a          781 c          856 g          442 t
ORIGIN      
        1 caaggtcgcc cgatgagcgc actgatgacg gtcgccgagc tggcgcggac ggtggcggcg
       61 caggacgtgc gtggggatgg acgcatcggc tttgcgagcg tatcgaccga tacgcgcacg
      121 ctgccaaacg gagccctttt cgtggcgttg cgcggcgaac gcttcgacgg gcacgactat
      181 gcgcaatcgg ccgctgatcg cggtgccgcc gcgctgctgg tggaacacgc cgtcgaagcc
      241 gcggtcccgc agatcattgt tgcggacacc aagcacgtgc tcgggctggg cgctgcacag
      301 tggcgggcgc gattcgcgct gccggtgatt gccgtgacgg ggagcaacgg caagaccacg
      361 gtcacccaga tgatcggggc gatccttgcg caggcccacg gcgagcgaca tcacctcgcc
      421 acactcggca acctgaacaa cgacatcggc gtgccgctga tgctgtggca gctcggcaag
      481 cagcacaagg cggccgtgtt cgagcttggg atgaatcatc cgggcgagat cgcttacctg
      541 gccgaactgg tccggccgac ggttgcgctt gtcaacaacg cgcagcggga acaccaggaa
      601 ttcatgcaat ccgtcgaagc cacggcccac gaaaacggcg aagtgctggc cgccctgcca
      661 ctggccgaca tgggcgtggc ggttttcccg ggcgatgatc cctgcacgcc gatctggcgc
      721 tcgctggctg gaacgcgacg cgtcgtcgat ttcgcgctcg aggcctatgc cgtcgtcacg
      781 gccacttacg aactcgacgc ggaaggcgcg cacgtttcga tggccacacc tgccggtctg
      841 gtcgaggcac ggctcgccgt gggcggtgtg cataacgtgc gcaacgcgct cgcggcggcc
      901 gcatgtgcga tggcggtcgg tatcgagccg cgcgatatca gcgccggcct ggccgccttc
      961 cgaccggtca atggacgcgg ggcgcgcctg cgcacgtcct cgggcgcgtg gctgatcgac
     1021 gacagctaca acgcgaaccc ggactcggtg cgggcggcca tcgatctgct cgccggttgt
     1081 gcctcgccgc gcgtgctggt gctgggcgac atgggggaag tcggcgaccg gggccccgag
     1141 tttcaccgcg aggtcggcat ctatgcccgc gagcgcgcca tcgatgtgct ctttgcactc
     1201 ggaacgcaga cgcgcgaaac ggttgccgcg ttcggcccag gcgggatgca tttcgacgat
     1261 gtcgacacat tgatggccgc gctgcccgca aaggtgacgg cgggctcgac cgtgcttgta
     1321 aaagggtccc gcttcatgcg gatggagcgc gtggtgcagg cgctggcgga accgtcggca
     1381 cacggcgctg cggcgggagc tcactgatgc tgctcgaact tttcgaatgg ctggcgcgcg
     1441 acatccgggc gttcaatgtc ttctcgtatc tgacgctgcg cgccgtgctg gcggccgcga
     1501 ccgcgctgct gctcgggctg gcggccggtc cgcgggtgat ccgccgtctg accgagctga
     1561 agatcggcca ggccgtgcgc agcgacggac cgcagacgca tctcgcaaag accgggacgc
     1621 cgacgatggg cggcgcgctg attctcatct cgatcggcat cacgacgctg ctgtgggccg
     1681 atctgtcgaa ccgcttcatc tgggtcgtgc tgatcgtgac gctgggcttc ggctggatcg
     1741 gctgggtcga cgactatcgg aaggtcgtgc atcgcaatcc gaagggcatg agcgcgaagg
     1801 agaagtactt ctggcagtcg ctcatcgggc tcggggccgc gttctacctg gcgtttgcga
     1861 tcgcggcgcc ctcgacggac aaggtctggt cgctgatggt gctgtgggtg cagcagggct
     1921 tttacatcga cctgccgcct cgctccgacc tgctggtgcc atttttcaag aacgtggcct
     1981 atccgctcgg cgtgttcggc ttcgtcgccc tttcgtaact cgtcatcgtc ggcaccagca
     2041 atgcggtgaa cctcaccgac ggcctcgatg gcctggcgat catgccgacg gtgatgatcg
     2101 gcgccgcgct cggcgtgttc gcctatgtca tcggacgcgc ggacttcgcg cggtacctgc
     2161 tgtgtcctta cattccgggg gccggcgagc tcgtgatcat ctgcggggca atcgccggcg
     2221 cgggactggc gttcctgtgg ttcaacgcct accccgccga agtcttcatg ggcgacgtcg
     2281 gcgcgctcgc cctcggcggc gcgctcggca cgatcgcggt gatcacgcgg caggaaatcg
     2341 tgctcttcaa catgggcggc gtgttcgtcg tcgagaccgt gtcggtgatg ctgcaggtgg
     2401 cctacttcaa gtggaccaag ggccggcgca tcttccgcat ggcgccgctt
//
LOCUS       DATIRT010000273         3640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_336082, whole genome shotgun sequence.
ACCESSION   DATIRT010000273 DATIRT010000000
VERSION     DATIRT010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336082"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..2020
                     /gene="htpG"
                     /locus_tag="VLN25_06240"
     CDS             110..2020
                     /gene="htpG"
                     /locus_tag="VLN25_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HSQ10197.1"
                     /translation="MGATDTAMKQTLGFQAEVKQLLQLMIHSLYSNKQIFLRELISNA
                     SDALDKLRFEAIAHSQLLEGHGELAIRVAFDKAARTITISDNGIGMSRQEAIDHLGTI
                     AKSGTREFFSQLTGDQQKDAQLIGQFGVGFYSSFIVADRVSVLTRRAGAAPGEAVLWA
                     SDGGGEFTIESAERAVSGTDVTLHLREGEDELLNGYSLKSIISKYSDHISSPIRMRGE
                     KWDKDKGEMVVADEDETVNQASALWTRAKSEISDEQYQEFYRHVSHDFGEPLAWTHSR
                     VEGRSEYTQLLYLPTRAPFDLWDRNQRHGIKLYVKRVFIMDDAEQLMPPYLRFVRGVI
                     DSSDLPLNVSREILQESRDVKAIREGSTKRVLSLIEDLAENQKEKYATFWKEFGQVLK
                     EGIGEDSANRDRIAALLRFASTASGGEEPTVSLRDYVARMKDGQDRIYYVTADTPEAA
                     RSSPHLEIFRKKGIEVLLLTDRVDEWMLSFLREFESKSLASVAKGGLELGALADETEK
                     AEQQQTADEYKDLVGKVKTALGDRVKDVRVTFRLTDSASCLVSDDQDISGHLQRLLKA
                     AGQKAPEARPILELNPRHVLVARLKAAEGDIADWSNLLFEQALLAEGGQLDEPALFVQ
                     RVNRLLLDVAAQ"
     gene            2004..2555
                     /locus_tag="VLN25_06245"
     CDS             2004..2555
                     /locus_tag="VLN25_06245"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HSQ10198.1"
                     /translation="MSQRSSGALRGQRVATPVPAAALRGFAPIIDDRVERLILGSFPS
                     AASLAAGQYYAHPRNQFWLILGRLLEEPLAELPYDERTRRVLSHRVGIWDVFGACERE
                     GSADTAIRNARPNAFKRLHRLAPRLHAIAFNGSAAGRFQPLFEQAGFAVRVLPSTSPA
                     HAGRSLEQKLALWAAWWRAPRGG"
     gene            2659..>3640
                     /locus_tag="VLN25_06250"
     CDS             2659..>3640
                     /locus_tag="VLN25_06250"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HSQ10199.1"
                     /translation="MSTTSTAGSGRASRAHAQRALVALRASMRSTLDPLLPTAAREAI
                     QVIDADAQASGTLKKSVRRLLVDQREFSSTFFSAVEREIDAAVDDLFRSDRDEKKAKE
                     AAEKTPAVALSLVDYDQMEENMLLDRFAARIRNAAEEQYGPLTQRLATALGVHGLSDR
                     ENPFHPVRFCRALGEAVDKLGFKGDQRLAVVKAFDGALGRPLTELYTELNRQLRDQGV
                     EERNTAATAFKNTMAGFRNTFVAAGRPSQGGGAVGAITGATAEQLLSALYQRMQVPMG
                     GAQVHGTPVVPITSVPTQPLFDRTDFAVPSPPIAPGAAGFGNTGAFPVGSA"
BASE COUNT          661 a         1149 c         1223 g          607 t
ORIGIN      
        1 tcggctcggc atccggggaa gggcatgtac ttgataattc gcctttggaa cccaatgtcc
       61 ggcccgcagc ggctcggccg ggcgatggca ttgatctagg gaggcaggga tgggtgcaac
      121 cgatacggcg atgaagcaga cccttggatt ccaggcggag gtcaagcagc ttttgcagct
      181 gatgatccac tcgctataca gcaacaagca gatcttcctg cgcgagctga tcagcaatgc
      241 gtccgacgcg ctcgacaagc tgcgtttcga ggcgatcgcg cattcacagc tgctcgaagg
      301 acatggcgaa ctggcgatcc gcgtcgcgtt cgacaaggcc gcgcgcacga tcacgatctc
      361 cgataacggc atcggcatga gtcggcagga ggcgatcgac catctcggga cgatcgcaaa
      421 gtcgggcacg cgtgaattct tctctcagct gaccggcgac cagcagaagg atgcgcagct
      481 gatcggccag ttcggcgtcg gcttctattc cagtttcatc gtggccgatc gcgtcagcgt
      541 gctgacccgg cgcgccggcg ctgccccggg cgaagccgtc ctgtgggcca gtgacggggg
      601 cggcgaattc acgatcgagt ctgccgaacg cgcggtgagc ggcacggacg tgacgctgca
      661 tctgcgcgag ggcgaggatg aactgctgaa cggctactca ctgaagtcga tcatttcgaa
      721 gtactcggac cacatctcgt cgccgattcg catgcgcggg gagaaatggg acaaggacaa
      781 gggcgaaatg gtcgttgccg acgaggacga aacggtcaac caggccagcg cgctgtggac
      841 gcgcgcgaag agcgagatca gcgacgagca gtaccaggag ttttaccggc acgtgtcgca
      901 cgacttcggc gagccgctgg cgtggaccca cagccgcgtc gagggccgca gcgagtacac
      961 gcagctgctc tacttgccga ctcgcgcgcc ctttgacctg tgggaccgca accagcgcca
     1021 tggcatcaag ctgtacgtga aacgtgtctt catcatggac gatgccgagc agctgatgcc
     1081 gccctacctg cgcttcgtgc gcggggtcat cgacagcagc gacctgccgc tcaacgtctc
     1141 acgcgaaatc ctgcaggaaa gccgtgatgt caaggcgatc cgcgagggct cgacaaagcg
     1201 cgtgctgtcg ctgattgagg acctggcgga aaatcagaaa gagaagtacg ccacgttctg
     1261 gaaggagttc gggcaggttc tgaaggaggg catcggtgag gattccgcca accgagatcg
     1321 gatcgcggca ctgttgcgtt tcgcgtccac tgcgtcggga ggcgaggagc cgacggtttc
     1381 gctgcgggac tatgtcgcgc gcatgaagga cggccaggac cggatctact acgtgaccgc
     1441 cgacaccccc gaggcggctc gcagcagccc gcacctggag atcttccgca agaagggcat
     1501 cgaagtcctg ctgctgaccg accgcgtgga tgagtggatg ctgtcgttcc tgcgcgaatt
     1561 cgaatcgaag tcactggcgt cggtcgccaa aggcggactc gaactcggcg cgctcgccga
     1621 tgaaaccgaa aaggccgagc aacagcagac cgcggatgag tacaaggatc tggtcggcaa
     1681 agtcaagact gcgcttgggg atcgcgtgaa ggacgtgcgc gtcacgttcc ggctgaccga
     1741 ttccgcgtca tgcctggtca gtgacgacca ggacatcagc ggccacctgc agcgcctgct
     1801 gaaggcggcc gggcagaagg caccggaggc gcgacccatc ctcgaactga acccccgtca
     1861 cgtgctggtc gcacgcctga aggctgcgga aggcgacatc gccgactggt cgaacctgct
     1921 gttcgaacag gcactcctgg ccgagggagg gcagctcgac gaacctgccc tgttcgtgca
     1981 gcgcgtgaat cgactactgc tggatgtcgc agcgcagtag cggtgccttg cgcgggcagc
     2041 gtgtcgccac gccggtgccc gccgctgcgc tgcgcggttt tgccccgatc atcgacgacc
     2101 gggttgaacg cttgattctt ggcagtttcc cgtctgcggc gtcgctcgcg gccggccagt
     2161 actacgcgca tccgcgcaac cagttctggc tcatcctcgg acgtctgctg gaagagccac
     2221 tggccgagtt gccgtacgac gagcgaaccc gccgcgtgtt gtctcaccgc gtgggcatct
     2281 gggacgtgtt cggtgcctgt gagcgggagg gcagcgccga cacggcgatc cgcaatgccc
     2341 gtcccaatgc cttcaagcgc ctgcatcgcc tggcgccgcg gctgcacgcc attgccttca
     2401 atgggtcggc agccggccga tttcagccgc ttttcgagca agcgggcttc gcggtacggg
     2461 tgctaccttc caccagtccc gcgcatgccg gacgcagcct cgagcagaag ctcgcgctgt
     2521 gggcagcatg gtggcgcgcg ccgcgcggtg ggtagatgcc gggccgagtg ctcggccggt
     2581 cgggcggcga gggctgactc cggtgtccgg gatcggctac ccttgcgtcc cgatgcgccg
     2641 gaggcataac gagtcggaat gagcacgaca agtacagcag gttcgggacg ggcgagccgc
     2701 gcgcacgcgc agcgcgcgct cgtggcgttg cgcgcctcga tgcggagcac gctcgatccg
     2761 ctgctgccca ccgcagctcg cgaagcgatc caggtcatcg atgccgacgc gcaggcgtca
     2821 ggcacgttga aaaagtcggt gcggcgcctg ctggtcgatc agcgcgagtt ctccagtacg
     2881 ttcttctccg ccgtcgagcg tgagatcgat gccgccgtcg acgatctgtt ccgatctgac
     2941 cgcgacgaaa agaaagccaa ggaagccgcc gagaagacgc cggccgtcgc gctctctctg
     3001 gtcgattacg accagatgga agagaacatg ctgctggacc ggttcgcggc gcgcatccgc
     3061 aatgcggccg aggagcaata cgggccgctg acgcagcgtt tggccacggc gctcggcgtg
     3121 cacgggctca gcgaccgcga aaacccgttt cacccggtgc gcttctgtcg tgcgctcggc
     3181 gaagcggtcg acaagctcgg gttcaagggt gaccagcgcc tcgctgtcgt gaaggccttc
     3241 gatggcgccc tcggacgtcc gctgaccgag ctgtacacgg aactgaaccg gcaattgcgc
     3301 gaccagggcg tggaggagcg caataccgcg gcgaccgcgt tcaagaacac gatggccggt
     3361 ttccgcaaca ccttcgtcgc agcgggtcgc ccgtcgcagg gcggtggagc cgtcggcgcc
     3421 atcaccggcg cgaccgcgga acaactcctc agcgcgctgt accagcgcat gcaggtgccg
     3481 atgggcggtg cccaggtgca cggcacgccg gttgtgccca tcacctctgt cccgacgcag
     3541 ccgctgttcg atcgcacgga ctttgccgtg ccttctcctc ccatcgcgcc tggggcagcc
     3601 ggtttcggca ataccggcgc gttccccgtc gggtcggccg
//
LOCUS       DATIRT010000274         3895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1713795, whole genome shotgun sequence.
ACCESSION   DATIRT010000274 DATIRT010000000
VERSION     DATIRT010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1713795"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VLN25_06255"
     CDS             complement(<1..466)
                     /locus_tag="VLN25_06255"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSQ10200.1"
                     /translation="MKSTQRKPRKAPSKRTAKSATAVAAPQKPVMALARQVRSWADTA
                     WGLAGSAADMSLALAKSSVGPKRRATIERAGALLRSMRETAGLTAQELGRAINLRDAT
                     LLEEAERGRIALPFEIILRLAAVLGRRDPVAFVMNLTRSYNPELWKALEDLGV"
     gene            745..2580
                     /locus_tag="VLN25_06260"
     CDS             745..2580
                     /locus_tag="VLN25_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSQ10201.1"
                     /translation="MGQTAEDLVTSLNSRDVQRRLQEYRALLANDAVGILAICDGEVM
                     QCNPSAARLFGWEPERLLGQSAAVFFSSDEEFQGFANRIGDALNAGGSPAMEWRVRRS
                     DGAAFWARLLVKSITPGGPGGETIWMVEDITARKAMETALSRVREELEQRVRSAHDEL
                     AWSNERLVAEMYERSEVEERARRMSLYDATTTLPNRRLLESRLDEAVRNHQMGGDRLA
                     VLVIDIDDFTKLNDALGHRVGDSLLQQVAGRLIETVRTSDLVARVGSDEFAVVLGRLR
                     HTDDAERVATKLAELLSEPYEVDAEQVTATVSIGVACFPTDGAGPEMLLRNADAALSY
                     AKSRGRANVQLFEPRMNSDLLRRLQLEAALRCAIDRSEFEVHFQPRINLSTGRVVGAE
                     ALLRWRHPEQGVLEPSSFIAVAEDSGLMQPIGEIVLRAACEAAKAWSTAGLGNLVVSV
                     NLSPREFRGRSLLPIVSQALIDSGLDAEYLEVEITEASLMRDLDKAEQVLVGLQAMGV
                     RIALDNFGTGYSSLSNLRRFPLNALKIDGQFVRAAPDDEADARIVAALAGLATGLGLH
                     VVAEGVETEQQFQFVKHCGCNEAQGYHLSRALPRDQFEHFARQRK"
     gene            2666..3046
                     /locus_tag="VLN25_06265"
     CDS             2666..3046
                     /locus_tag="VLN25_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HSQ10202.1"
                     /translation="MNKEIVSTPAAPAAIGPYSQAVKTGLVVFTSGQIGLDPRTGEMV
                     SAEFEPQVRQAFANLDAVARAAGGSLAHAVKFSLFLTDLSKFATVNQIMSELVPKPYP
                     ARSTVGVASLPKGAQFEVEAVLVL"
     gene            3127..>3895
                     /locus_tag="VLN25_06270"
     CDS             3127..>3895
                     /locus_tag="VLN25_06270"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HSQ10203.1"
                     /translation="MAARLAAIGLRSDWDFIFHLPLRYEDETVVVPIDALSEGGESQI
                     EATVTAADVVFRGRRQLHVTVQDASGTLVLRFLHFYPSQLKQLALGQRIRAFGASRGG
                     LAGMEMVHPRVRSVREGEALPNALTPIYPTTAAVPQGWLRRRIARALRDVELRDLLPD
                     SLRERFNLEPLRPALERLHPPPPAADAPALAERTDPAWQRLNFDELVAQQIALRLARA
                     ARQKRSSPALRPDGLTGRLLAALPFALTAAQRRVWAEI"
BASE COUNT          617 a         1281 c         1350 g          647 t
ORIGIN      
        1 caacgccaag gtcctcgagc gctttccaca attccgggtt gtaggagcgc gtcagattca
       61 tcacgaacgc caccgggtcg cgccggccga gcacggcggc gagccgcagg atgatttcaa
      121 acggcagggc aatccgtccc ctttcagcct cttcgagcaa agtcgcgtcg cgcaggttga
      181 tggcgcggcc gagttcctgt gccgtcaggc ccgccgtctc gcgcatgctc ctgagcaggg
      241 cgccggcccg ttcaatcgtc gcgcgccgct tcgggccgac cgaggacttg gcgagcgcga
      301 gcgacatgtc ggcggcactg ccggccagcc cccaggcggt gtcggcccag gacctcacct
      361 gccgagccag cgccatcacc ggcttctggg gtgcggccac tgccgtggcc gactttgcgg
      421 tgcgtttgct gggagccttt cggggcttgc gttgcgtcga tttcatggct ggttcgagtg
      481 ggttccgggg tgggaggcga agcgcgaact ggccgggttt ccgcggcatc ttgaacgccg
      541 ccgcgggcaa gcgcaagcag caccacgacg cgctgcgtgg cgtgagccgt ctgcgccggg
      601 ttcagccgag tggctgatgg cactcgaata gccgggctgg ctcggccgta atccgacctt
      661 cgcgggcgcg ctacgcgcgg aaactaccct tagccgcgcg ctgacgatcg tgcggaattt
      721 cgagctatcg ggattgggga ttgcatgggc cagaccgccg aagaccttgt tacctcactg
      781 aacagccgcg acgtgcaacg ccgcctgcag gagtaccgcg cgctgctggc caacgacgct
      841 gtcggcatcc tggcgatctg cgacggcgaa gtgatgcagt gcaatccgag cgccgcgcgc
      901 ctgttcggct gggagccgga acggctgctc ggtcagtcgg cggccgtgtt cttctcgtcg
      961 gacgaggaat tccagggatt cgccaatcgc atcggcgatg cgctcaacgc cggtggctcg
     1021 cccgcgatgg agtggcgcgt ccgccgcagc gatggcgctg cattctgggc gcgactcctg
     1081 gtgaaaagca tcacgcccgg cggcccgggc ggtgaaacga tctggatggt cgaggacatc
     1141 acagcgcgca aggcgatgga aacagcactg tcgcgcgtgc gcgaggaact cgagcagcgt
     1201 gtgcgctccg cgcacgatga actcgcctgg tcgaacgaac gcctcgtcgc cgagatgtac
     1261 gagcgcagcg aggttgagga acgggcgcga cgcatgtcgc tgtacgacgc gaccaccacg
     1321 ctgcccaacc ggcggctgct cgagtcacgc ctcgacgaag cggtgcgcaa ccatcagatg
     1381 gggggagatc ggctggcggt gctggtcatc gacatcgacg acttcaccaa gctcaacgac
     1441 gcgctcgggc accgcgtcgg cgattcgctg ctgcagcagg tcgcgggccg gctgatcgag
     1501 acagtacgga cgtcggacct ggtggcgcgc gtcggctcgg acgagttcgc ggtcgtgctc
     1561 gggcgcctgc gccacaccga tgatgccgaa cgcgtggcga ccaaactcgc cgaactgctg
     1621 tcggaaccct acgaagtcga tgcggagcaa gtgacggcga cggtcagcat cggcgtggcc
     1681 tgcttcccaa ccgacggtgc cggcccggaa atgctcctgc gcaatgccga tgcggcgctg
     1741 tcgtatgcga agtcgcgggg ccgcgccaat gtccagttgt tcgagccgcg catgaattcg
     1801 gatctgctgc gtcgcctcca actcgaggcg gcgctccggt gcgcgatcga caggagcgag
     1861 ttcgaagttc atttccagcc ccgcatcaac ctgtcgaccg gccgggtcgt tggcgccgaa
     1921 gcgctgctgc gctggcgtca tccggaacag ggtgtgctcg agccgtcgag cttcatcgcg
     1981 gtggccgagg atagcggcct gatgcagccg atcggcgaga tcgtgctgcg agcggcctgc
     2041 gaagcggcga aggcctggag cacggccgga ctgggcaacc tggtcgtcag cgtcaacctt
     2101 tcgccgcgcg aattccgcgg gcgctcgctg ctgccgatag tgagccaggc gctgatcgac
     2161 tcggggctcg acgccgagta tctcgaggtc gagatcaccg aagccagcct gatgcgcgat
     2221 ctcgacaagg ccgagcaggt cctggtcggc ctgcaggcga tgggcgtgcg catcgcactc
     2281 gacaacttcg gcacgggtta ctccagcctt tcgaacctgc gacgctttcc gctcaatgca
     2341 ctgaagatcg atggccagtt cgtgcgcgcc gcaccggacg atgaagccga tgcgcgcatc
     2401 gtggcggctc tggcgggtct ggcgaccggg ctgggcctgc acgtcgtggc cgagggcgtc
     2461 gagacagagc agcagttcca gttcgtcaag cactgtggtt gcaacgaggc gcagggctat
     2521 cacctgagcc gcgcgctgcc gcgcgaccag ttcgagcact tcgcccgcca gcgcaagtag
     2581 ccaactggca ctttctcttg cagcgaatgc gctgcgcgtg gcggctagac tgcgcccttc
     2641 gtcgatgtgc gccgaagggg ccgccatgaa caaggaaatt gtctccacgc ctgcggcgcc
     2701 ggccgcgatc gggccgtact cgcaggccgt gaaaacgggc ctcgtcgtgt tcacctcggg
     2761 ccagatcggc ctggatccca ggacggggga aatggtcagc gcggagttcg agccacaagt
     2821 gcgccaggcc tttgcgaacc tcgatgccgt tgcgcgcgcc gcgggtggtt cactggccca
     2881 tgcggtcaag ttctcgctct tcctcaccga cctgtcgaag ttcgcgacgg tcaaccagat
     2941 catgagcgaa ctggtcccca agccctatcc ggcgcgctcc accgtcggcg tggcaagcct
     3001 gcccaagggt gcacagttcg aagtcgaggc ggtgctcgtc ctctagcggc ggcgactatc
     3061 ttggcacgcg cagcctcggc acgcgcaggc gcggcgcaga cggcgaccgg tcgcggtggc
     3121 cgtgacatgg ccgcgcgtct tgccgcgatc ggcctgcgca gcgactggga cttcattttt
     3181 cacctgccgt tgcgctacga agacgagacg gtcgtcgtgc cgatcgacgc gctcagcgag
     3241 ggcggcgaat cgcagatcga ggcgaccgtc acggccgccg atgtcgtctt ccgtggccgt
     3301 cgccagttgc acgtcaccgt gcaggatgcc agtggcacgc tggtcctgcg cttcctgcac
     3361 ttctacccgt cgcagctgaa gcaactggcg ctcggacagc gcatccgcgc gttcggcgca
     3421 tcgcggggcg ggcttgctgg catggagatg gtgcatccgc gtgttcgcag tgtgcgcgaa
     3481 ggcgaggcgc tgccgaatgc gctgacaccg atctatccga ccacggctgc tgtcccacag
     3541 ggctggctgc gccgacgcat agcgcgcgcg ctgcgagacg tcgagctgcg cgacctgctg
     3601 cccgactcgc tgcgggagcg tttcaacctc gagccactgc ggcccgcact cgagcgcctg
     3661 caccctccgc cgccggctgc tgatgcaccc gcgctggccg aacgcacgga ccccgcatgg
     3721 cagcggctta atttcgacga gctggtggcg cagcagatcg cgctgcggct ggcgcgtgcg
     3781 gcgagacaga agcggagctc gccggcgttg cgaccggacg ggctcacggg acgtctgctc
     3841 gcggcacttc cgtttgcgct aaccgcggcg cagcgtcgcg tgtgggccga gatcg
//
LOCUS       DATIRT010000275         1752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1811897, whole genome shotgun sequence.
ACCESSION   DATIRT010000275 DATIRT010000000
VERSION     DATIRT010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1811897"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1578)
                     /locus_tag="VLN25_06275"
     CDS             complement(<1..1578)
                     /locus_tag="VLN25_06275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HSQ10204.1"
                     /translation="MLLPSRLFALRPIAAALASAASVLLVTSQPTFAQAPDPAKDTQQ
                     LLEEARRMVKNDLLQIYREAIANDAAFAAARYQQQASQERVPQARSALLPNIGLTGRT
                     DANKYSTNEPDINNTYSVYGGGVNLSVPLYRPQNWEALEQAKIAVMQADLTLQQSAQD
                     LTLRVATAYFNVLAAVDQLIALDAAKRATLEQLQQARREFEVGTKTIIDTNEAQARYD
                     QIVAQEQVALGTLLVRRSELQTIIGRDLGTLAPLRDRPNLTLPQPNDIMQWVRSAEDT
                     SYTVQIARASYEIASREVQRQRDGHKPTVDVVGGYNVNKFNGTQTSDANPRINAGTIG
                     LQLNVPIYQGGLIQSRVREALSLQDRSNAELESARRNSANAARDAYTGVNYGLAQVKA
                     LESAEVSARTQLDSTQLGYQVGVRILLDVLNATTQLVQTQRDLKRARYDFLLAALRLK
                     ASTAALTDEDVAGVNALLEDKAMPLTVPDVLAPERTPRSLVPPANPPARTAPTSGTSA
                     TPTVASSGGSPSSTAKPT"
     gene            complement(1595..>1752)
                     /locus_tag="VLN25_06280"
     CDS             complement(1595..>1752)
                     /locus_tag="VLN25_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HSQ10205.1"
                     /translation="TTRPIVCVCHHGGRSMHVAMFLARQGFENVYNLTGGVDAWARQV
                     DAAMPTY"
BASE COUNT          259 a          538 c          607 g          348 t
ORIGIN      
        1 cgtcggcttg gccgtgcttg agggcgagcc gccgctgcta gcgaccgtag gcgtcgcgct
       61 ggttccgctg gtgggtgccg tacgggcagg cggattcgcc ggcgggacga gcgacctcgg
      121 ggtgcgctcc ggtgcgagta catccggcac cgtcagtggc atggccttat cttctaaaag
      181 agcattcacg ccggccacgt cttcgtcggt gagcgcggcc gtcgacgcct tcaggcgcaa
      241 cgccgcgagc aggaagtcat agcgggcccg cttgaggtcg cgctgtgtct gtaccagctg
      301 cgtcgtagcg ttcagcacgt cgagcaggat tcgaaccccg acctggtagc caagttgcgt
      361 cgagtcgagc tgcgtgcggg ccgagacctc ggccgattcg agtgccttga cctgggccag
      421 gccgtagttc accccggtat aggcgtcgcg cgctgcattc gcggaattgc gtcgggccga
      481 ttcaagttcg gcattggagc ggtcctgaag cgaaagggct tcgcgcacgc ggctttggat
      541 gagtccgccc tggtagatcg gaacgttcag ctgcagcccg atggtgccgg cattgatgcg
      601 cggattggcg tcactggtct gagtaccgtt gaacttgttg acgttgtaac cccccacgac
      661 gtcgaccgtg ggcttgtggc catcgcgctg gcgctgcact tcgcgcgacg ctatctcgta
      721 gctcgcgcgg gcgatctgca ccgtatagct ggtgtcctcg gccgagcgca cccattgcat
      781 gatgtcattg ggctgcggca gcgtgaggtt gggccggtcg cgcagcggcg ccagcgtgcc
      841 gagatcgcga ccgatgatgg tctgcagctc actgcgccga acaagcagcg tgccgagcgc
      901 cacctgctcc tgcgccacga tctggtcgta gcgtgcttgc gcctcgttgg tgtcgatgat
      961 ggtcttggta ccgacttcga actcacgcct cgcctgctgc agctgctcga gcgtggcgcg
     1021 cttcgcggcg tccagggcga tcagctgatc taccgcagcc agcacattga agtaggctgt
     1081 cgccactcgt agcgtcaggt cctgggccga ctgctgcagc gtgagatcgg cctgcattac
     1141 ggcgatcttg gcctgctcga gcgcttccca gttctgcggg cggtacagtg gcacggacag
     1201 gttcacgccg ccgccgtaca cgctgtaggt gttgttgatg tcgggctcgt tggtgctgta
     1261 cttgttcgca tcggtgcggc ccgtgagccc gatgttgggc agcagtgccg agcgcgcctg
     1321 cggcacccgc tcctgtgaag cctgctgctg gtaccgagcg gcggcaaagg cggcatcgtt
     1381 ggcgatggcc tcgcgataga tctgcagcag gtcgttcttg accatgcgcc gtgcttcctc
     1441 gagcagctgc tgtgtgtcct tggccggatc cggtgcctgt gcaaaggtcg gctggctcgt
     1501 gaccagcagg actgaggcgg cactcgccag cgccgcggcg atcggccgca gtgcaaacag
     1561 gcgggaagga agcaacatgg tggtctctcc agcttcagta ggtaggcatc gcggcatcga
     1621 cctggcgtgc ccaggcatcg acgccgccag tcaggttgta gacgttctcg aatccctgcc
     1681 gcgccaggaa catcgccacg tgcatgctgc ggccgccgtg atggcagacg cagacgatgg
     1741 ggcgcgtggt at
//
LOCUS       DATIRT010000276         2750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_337187, whole genome shotgun sequence.
ACCESSION   DATIRT010000276 DATIRT010000000
VERSION     DATIRT010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337187"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VLN25_06285"
     CDS             <1..402
                     /locus_tag="VLN25_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HSQ10206.1"
                     /translation="ESSRFGAPIKKLGLVMAYPEMAPLVRLAGPDVALEILLEGRIFD
                     AAEAKDKGLVTRVVPDDQVATEVHATAHRIAEGAPLVARWHKKFARRLAEGGTLTAQE
                     LDECFDCFDTEDFRIGYSAFLAKRKPDFVGR"
     gene            408..1643
                     /locus_tag="VLN25_06290"
     CDS             408..1643
                     /locus_tag="VLN25_06290"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HSQ10207.1"
                     /translation="MDAAQPVPDRPAHSGPLAGMRVLELAQIMAGPTAGMMLADMGAD
                     VIKVETLPGGDDSRTYREPRVNGVSAPFMILNRNKRGIALNLKLPAGRDILLRMVRDA
                     DVLTENYRRGTLEKLGLGYDVLSKVNPGLVYCAVSGYGRDGPYGDKGGFDLIAQGFAG
                     LMSITGEPGGPPAKTGNSVADINAGILAVVGILAAYTHKQKTGQGQMVETSLMEAALQ
                     QTYWHAAIQFATGTSPGPTGSAHILTAPYQAFHARDGWLNIGGANQANWERIADVLGH
                     PEWRDDLRFSTNSDRMSNLDVLVGKINEVLGTRNRSEWIEAFDAAGVPAGPVHSIGEA
                     LSHPQTLARGMVVDLVHPQAGATKAVGCPVHFSATPTAITRPAPLLGEHTRALLHDYG
                     YSDAEIEGFVAEGAVEASS"
     gene            2428..>2750
                     /locus_tag="VLN25_06295"
     CDS             2428..>2750
                     /locus_tag="VLN25_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10208.1"
                     /translation="MRQRLLRGQPEVGENARVTTLPQRQQPVDQRPAQSTASDLAETG
                     ASTEIQFAARLVPASTTIVLASRPGATGTSIEHSSNYHEEQVSVHAPIEVTGDDIERA
                     LFEQS"
BASE COUNT          494 a          919 c          870 g          467 t
ORIGIN      
        1 gagtcctccc gcttcggcgc gccgatcaag aaactgggcc tggtcatggc ctacccggag
       61 atggcgccgc tggtgcgcct tgccgggccg gacgtcgcgc tggagatcct gctcgaaggc
      121 cggatcttcg acgccgccga agcgaaggac aagggcctgg tgacgcgcgt ggtgccggac
      181 gaccaggtcg cgactgaagt ccacgccacc gcccaccgca tagcggaagg cgcccctctg
      241 gtcgctcgct ggcacaagaa gttcgcacgg cgccttgccg aaggcggaac gttgacggcc
      301 caggaactag acgagtgctt tgactgcttc gataccgaag atttccgcat cggctattcc
      361 gcgtttcttg ccaagcggaa gcccgacttc gtcgggcgct agagccaatg gacgccgcgc
      421 agccggtacc ggaccgtccg gcccactccg ggccgctagc cggaatgcgg gtgctcgaac
      481 tggcgcagat catggccggc ccgaccgcgg gaatgatgct cgcggacatg ggcgccgatg
      541 tcatcaaggt cgagacgctt ccaggcggcg acgattcgcg cacgtatcgg gagccgcgcg
      601 tcaacggcgt gtctgcgcct ttcatgatcc tgaatcggaa caagcgcggc atcgcattga
      661 acctcaagct gcccgccggg cgtgacatcc tgctgcgcat ggtccgcgac gccgatgtcc
      721 tgacggaaaa ctaccggcgc ggcacgctcg agaagctcgg actcggctac gacgtgctgt
      781 cgaaagtgaa tcccggcctg gtttactgcg cagtttcagg gtacggccgt gacggtccgt
      841 acggggacaa gggcggattc gacctgattg cacagggctt cgcgggactg atgtcgatca
      901 ccggcgagcc cggcggcccg cccgcgaaga ccggcaactc ggtggccgac atcaacgccg
      961 gcattcttgc ggtcgtcggc atcctcgccg cctacacgca caaacagaaa accggccagg
     1021 gacagatggt tgaaacgtcg ttgatggaag cggcactgca gcagacgtac tggcatgcag
     1081 cgatccagtt cgcgaccggc acctcccctg gcccgacggg ctcagcgcat attctgacgg
     1141 ctccctacca agcatttcac gcccgggacg gctggctcaa catcggcggc gccaatcagg
     1201 cgaactggga acgcatcgcc gacgttctcg ggcatccgga gtggcgcgac gatttgcgct
     1261 tcagtacgaa ctccgaccgc atgtccaatc tcgatgtcct ggtggggaag atcaacgaag
     1321 tccttggaac gcgcaaccgg tccgagtgga tcgaggcatt cgatgcggcc ggcgttcctg
     1381 ccgggccggt gcacagtatc ggcgaagcgc tctcgcatcc tcagacgctg gcgcgcggca
     1441 tggtggttga cctcgtgcac ccgcaggccg gggcgacgaa agccgtcggc tgcccggtgc
     1501 atttctcggc cacacccact gcaatcactc ggccggcgcc gctgctgggc gaacacacgc
     1561 gcgcgttgct acacgactat ggctacagcg atgcagagat cgagggcttc gtcgcggaag
     1621 gtgcggtcga ggcatcgtcc tagaaactac gattgcccga tgccggcaca tgcgccgaca
     1681 tatcgcgttt agacgtcttg acctggtcgc tgtaagcggg agaattagcc ggcggacctc
     1741 gccgaggacc gctggtttcc tgcaagcaac cgacggagcg ccatgaagcg aggctggatt
     1801 tcatggctag tgagcgcggc gtttctgctc ggcgcaaccg cggccacagc acagcccttg
     1861 tcttgcgcct cggtttcgcc cgaggcgcgc gaatatgtcc ggagccgagg cgcttgccgc
     1921 gatgccaaga ctgcatcgcg acccagagcg tccacgcagt ccaaagccag cgtttccggt
     1981 agcccgactt ccgcgcccga gacatacttc gtaccggatg tgatcggccg cggctatgcg
     2041 cacgcttcgc gcacgctggc cgaattcaaa gtcgaacgaa ttgaaacggc cagcgctgcc
     2101 ccagcgggtg aagtgcttgc gcaggacccc gcacccgccg ctcccgccct cccaggttcc
     2161 accgtcatcc tgcgggtatc ggatggctcg cttgccggcg ctgcgagtac gaatccggtt
     2221 gcggcgccgg ccactgcggc tgcggcctcg tccgtgccga cgcccgcaac gagccctgcg
     2281 cctgcctcga cgatcctccc tttgcctcct caagagccaa tcgatccgcc cggagcacgg
     2341 ggtcaattca cgaccgcatt ctgggctatc gctgcgctca ttttcggcgc tggcgcttcg
     2401 ctcggactgt tgtccggcgc actcctgatg cgtcagaggc tgctacgcgg ccagccggag
     2461 gttggcgaga acgcgcgggt gactacgctt cctcaacgtc agcagcccgt cgatcagcgg
     2521 ccggcccaat ccaccgcctc cgacctcgct gagaccggcg catctactga aattcaattc
     2581 gcagctcggc tcgttcccgc ctcgactacg atcgtgctgg cgtctcgtcc gggcgccact
     2641 gggacctcaa tcgagcattc gagcaactat cacgaagagc aggtcagtgt gcacgcgccg
     2701 atcgaggtca ccggcgatga catcgaaagg gcgctgttcg aacaaagcaa
//
LOCUS       DATIRT010000277         1570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_140023, whole genome shotgun sequence.
ACCESSION   DATIRT010000277 DATIRT010000000
VERSION     DATIRT010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140023"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VLN25_06300"
     CDS             <1..367
                     /locus_tag="VLN25_06300"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSQ10209.1"
                     /translation="TTFVGGRRNPARAVASDEELLQMVQYELERLVAVCGTPLFNEIT
                     RWPRAIPQYTIGHLQRIAQVERAERELPGLYFCANYRGGVSISDCITNGEGVARTVSK
                     YLAGATPMSTRGAGAACID"
     gene            426..>1570
                     /locus_tag="VLN25_06305"
     CDS             426..>1570
                     /locus_tag="VLN25_06305"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HSQ10210.1"
                     /translation="MAESESEVAPRHADVGALAQPEVTLSSTVIPLRQSAVERSLKQA
                     SFDTAGVPILAVNHSGQILAINSEAMVLVGGESVVGRSVAELLGFLSHRAFTRRWQRL
                     WSQVTAEGRCEFEAKLPLRSGARLSARFVVRLFRFDQDDFASISIVDTSAERAARLRV
                     RAAEARARALALHQSELTIVLNADRRVVFANAALEPITGEHEADIMGSPFEFILDQPS
                     RIEFRRAVDRLLREPAGDRQPTSLHLRTRAALAGNGRSLSCVLANRLDDSTVRGLVLT
                     ARDLGPAEKAQARRLRFRERLLELAIQPKSDFTQTLATVLRSTAEVLDVSSASFWRLS
                     RDPDVLRCEALFDRTADRFLRDWVGREFPATRFAAYFAQLMNRQPVVV"
BASE COUNT          243 a          518 c          529 g          280 t
ORIGIN      
        1 caccaccttt gtcggagggc ggcgcaatcc ggcgcgggcc gttgcgtcgg acgaagaact
       61 gctgcagatg gttcagtacg agctggagcg attggtcgcc gtgtgcggga caccgctctt
      121 caacgagatc acgcgctggc cacgcgccat tccgcagtac accatcggcc atctgcagcg
      181 aatcgcgcag gtcgagcggg ccgaacgaga gctgcccggc ctttacttct gcgcgaacta
      241 tcgtggtggc gtctccatca gcgactgcat cacgaacggc gagggcgtgg cgcgcacagt
      301 gtcgaagtac ctggcaggtg ccaccccgat gtcgacccgg ggagcaggcg cggcgtgcat
      361 cgattgatgc ggaccgttgg accgggtcat ttcgtccaat cggatgccct gccgaacgcg
      421 atggcgtggc agaatcagaa tcggaagtcg cgccccgtca tgccgacgtc ggggcgcttg
      481 cacagcccga ggtcacgctg agttcgaccg tcattccgtt gcggcagtcc gctgtcgagc
      541 gctcgctcaa gcaggcgagc ttcgataccg cgggcgtgcc gatcctcgct gtcaatcaca
      601 gtggccagat cctcgcgatc aactccgagg cgatggtgct cgtcggcgga gaatctgtgg
      661 tcgggcgcag cgtcgccgag ttgctgggat ttctcagcca ccgcgccttc acacgccgtt
      721 ggcagcgcct ctggtcgcag gtcaccgcgg aagggcgctg cgaattcgaa gccaagctgc
      781 ccctgcgcag cggcgcacgc ctgagcgcgc gcttcgtcgt ccggttgttc cgcttcgacc
      841 aggatgactt cgcctcgatc tccatcgtgg acacgtcggc cgagcgggcc gcacgtcttc
      901 gcgtgcgcgc tgccgaagcg cgggcgcggg cgctggcgct gcaccagagc gaactgacga
      961 tcgtcctgaa tgccgatcgt cgggtggtct tcgccaatgc ggcgctcgag ccgatcaccg
     1021 gcgagcacga agcggacatc atgggctcgc cattcgagtt catcctcgat cagccgtcgc
     1081 gcatcgaatt tcgccgcgcg gtggatcgcc tgctgcgtga gccggccggc gaccggcagc
     1141 ccacgtcatt gcatctgcgt acgcgcgctg cactggccgg caacggccgg agcctgtcat
     1201 gcgtactggc gaaccggctg gacgactcga ccgtacgtgg gttggtgctg accgcgcgcg
     1261 accttggacc ggctgagaag gcgcaggcgc gacggctgcg gttccgcgag cgcctgctcg
     1321 agctggcgat ccagccgaag tcggatttca cccagacgct ggcgaccgta ctgcggtcga
     1381 ccgccgaagt gctggacgtg agttcggcga gtttctggcg cctctcgcgc gatcccgacg
     1441 tgctgcgctg tgaggcactg ttcgatcgaa ccgccgaccg cttcctgcgc gactgggtcg
     1501 gccgagagtt tcctgcgacc cggttcgccg cttacttcgc gcaactgatg aatcgccagc
     1561 cggtcgtcgt
//
LOCUS       DATIRT010000278         3654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1909682, whole genome shotgun sequence.
ACCESSION   DATIRT010000278 DATIRT010000000
VERSION     DATIRT010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1909682"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..858
                     /gene="menB"
                     /locus_tag="VLN25_06310"
     CDS             16..858
                     /gene="menB"
                     /locus_tag="VLN25_06310"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HSQ10211.1"
                     /translation="MKRTTLPPIPAWTLVAGYSDILYEKSDEGIAKITINRPEVRNAF
                     RPETVKEMQSAFADARDDENIGVIILTGQGKEAFCSGGDQRVRGKGGYVGSDGVPRLN
                     VLDLQRQIRTLPKPVVAMVAGYAIGGGHVLHMICDLTIAADNARFGQTGPKVGSFDGG
                     YGASYMARLVGQKKAREIWFLCRQYDAKAALDMGLVNCVVPYEQLEAETVQWCREMLA
                     NSPIALRCLKAALNADCDGQAGLQELAGNATLLFYLSEEGQEGRNAYLEKRKPDFRKF
                     PRLP"
     gene            927..2249
                     /locus_tag="VLN25_06315"
     CDS             927..2249
                     /locus_tag="VLN25_06315"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSQ10212.1"
                     /translation="MSALLSIRAAAREAGNAVALRVGDQRYRYADLARLAEPHVNALA
                     SRGQAPSPLVGTNTLETLVMLYALLEARRPTLLLHPKLTAAERAIELAATAQAAATVP
                     EDAAAILYTSGTTGRARGAVLTRAALLASAQASSANLGWQEDDCWLLAMPLARVGGLS
                     IITRCLAARRTVALAPAFDAARLPQWIEEHRVTLLSLVPTMLTQVLDTHPEWRPPAHL
                     RVLLLGGAEASPALLERAACRGLPIVITYGCTETCSQVVATPYARRFDAAACGAGRPL
                     PGAQVRVDGGHILVRGPMRMAGYLAEPPLEPDTWFDTGDLGEFDADGFLHLHARRADL
                     IVSGGENVYPAEVERVLEGCTGIQAAGVFGLKDETWGQVVAAALVTGSPAPDDDELIE
                     YLATRLAPHKRPRWISVVQSLPVTPAGKLDRVALAALATTLRPLHRRP"
     gene            2386..3318
                     /gene="menA"
                     /locus_tag="VLN25_06320"
     CDS             2386..3318
                     /gene="menA"
                     /locus_tag="VLN25_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HSQ10213.1"
                     /translation="MSSSTTASSAAVVPGSLAAWSIACRLPSLLVAVSPVLVGTALAW
                     ERTGQFDAATMLLALACAVLLQVISNLQNDVGYTVRGAERHGNRTGLPRATANGWLGV
                     QQVRMAIVICCGLTMTLGSPLVLAHGWPVLAMGLGSLVAALAYMGGPRPVAYTPYGEL
                     VVFVFFGLVAVAGSDYVQTGSVGTTTWLAGIALGALAASALAVNNHRDVAHDRSVGRR
                     TFAVVFGDSTSRAMYALSVFGAFVLLVPMAWLSSPALLTPLLLAPVALRLQRDFARSA
                     PGLAYNAILVRTFKLELGYAALLAAGTVVGRLAA"
     gene            complement(3344..>3654)
                     /locus_tag="VLN25_06325"
     CDS             complement(3344..>3654)
                     /locus_tag="VLN25_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10214.1"
                     /translation="APDLIGRDGDSAVICAEVAASEEPVGVIDAQMRRALAKLASMDG
                     RRFYFVRSMSMKRRAATKVIKAGWDIAVVQIALLNSTQPTPAATPMTSLAAVGAGSPR
                     "
BASE COUNT          562 a         1266 c         1233 g          593 t
ORIGIN      
        1 cccgcagaga caacgatgaa acgcaccacc cttccgccga tcccggcgtg gaccctcgtc
       61 gccggctact cggacatcct ttacgagaag tccgacgaag gcatcgccaa gatcacgatc
      121 aaccgcccgg aggtgcgcaa tgcatttcgg cccgagaccg tgaaggaaat gcagtcggcc
      181 ttcgccgatg cgcgcgacga tgaaaacatc ggcgtcatca tcctgaccgg ccagggcaag
      241 gaagcgttct gctccggcgg cgaccagcgc gtgcgcggca agggcggcta tgtcggcagc
      301 gacggcgtac cacgcctgaa cgtgctcgat ctgcagcggc agatccgcac gctgcccaag
      361 ccggtggtcg cgatggtcgc cggctacgcg atcggcggag gacatgtact tcacatgatc
      421 tgcgacctga cgatcgcagc cgacaatgcg cgcttcggcc agaccggacc gaaggtcggc
      481 agtttcgacg gcggctacgg cgcgtcgtac atggcgcgcc tcgtcggcca gaagaaagcg
      541 cgagagatct ggttcctgtg tcgccagtac gacgcgaagg ccgcgctcga catgggactg
      601 gtcaactgcg tagtgcccta cgagcaactc gaggccgaga ccgtgcaatg gtgccgcgag
      661 atgctcgcca atagcccgat cgccctgcgc tgcctgaaag cggcgctgaa cgccgactgt
      721 gacggacagg cgggtctgca ggaacttgcg ggcaatgcga cgttgctgtt ctacctgtcc
      781 gaggagggcc aggagggccg gaacgcctac cttgaaaagc gcaagcccga cttccgcaag
      841 ttcccgcgtc tgccgtgacg gcgaagcgcg cgccggcgtg cgacgagctc tgagcgcggg
      901 ccgcccagcc ctgcactgtt gaacacatga gtgcgctgct gtcgatccgc gccgcggcgc
      961 gcgaagcggg caatgccgtc gcgctgcgcg tcggcgatca acgctaccgc tatgccgacc
     1021 tggcacggct cgcggagccg cacgtgaacg ctctcgcttc gcgcgggcaa gcgccgtcac
     1081 cgctggtcgg caccaacacg ctcgaaacgc tggtcatgct gtacgcgctg ctggaggcac
     1141 gccgtcctac tctactgctg cacccgaaac tcacggccgc cgagcgcgcg atcgagctag
     1201 cggccaccgc gcaggcggcc gccacggtgc cggaagacgc cgctgcgatc ctctacacat
     1261 cgggcaccac cggacgcgcg cgcggtgccg tgctcacgcg cgccgcgctg ctcgcctcgg
     1321 cgcaggcgag cagcgccaat ctcggctggc aggaggacga ctgctggctg ctggcgatgc
     1381 cgctggcgcg cgtgggcggc ctgtcgatca tcacccgctg cctggcagca cggcgcacgg
     1441 tcgcgctggc accggcgttc gacgccgccc ggctgccgca atggatcgag gagcatcgcg
     1501 tcacactgct ttcgctggtg ccgacgatgc tgacgcaggt cctggacacg catcccgagt
     1561 ggcgcccgcc ggcgcacctg cgggtgctgc tgctgggcgg ggccgaagcc tcaccggcct
     1621 tgctcgagcg cgcagcgtgc cgtggcctcc cgatcgtgat tacctacggc tgcaccgaaa
     1681 cctgttcgca ggtggtcgcc acgccctatg cgcgtcgctt cgatgccgcc gcgtgcggcg
     1741 ccgggcgccc gcttccgggt gcgcaggtgc gcgtcgacgg tggccacatc ctggtgcgcg
     1801 gaccgatgcg gatggccgga taccttgccg agccgccgct cgagcccgac acctggttcg
     1861 acaccggcga cctcggagaa ttcgacgcgg acggtttcct gcacctgcac gcgcggcgcg
     1921 ccgacctgat cgttagcggc ggcgagaacg tctatccagc cgaggtcgag cgcgtgctgg
     1981 agggctgcac cggcattcag gcggcgggcg tgttcggact gaaggacgag acctggggtc
     2041 aggtcgtggc cgctgcactg gtgacggggt cgccggcgcc ggacgatgac gaactgatcg
     2101 aatacctggc cacgcgactg gcgccgcaca agcggccgcg ctggatcagc gtcgttcaga
     2161 gcctgccggt caccccggcg ggaaagctcg atcgcgtagc gctcgcagca ctggcaacca
     2221 cgctgcggcc tttgcaccgg cgaccttgag aggtggtcga cgggcaccgg gccgccacgc
     2281 gattcgcggc gaattgactt aacactgaat ccgggcggat ttccactccc ccgcgcgccg
     2341 cgtgttcggg ataatgcgca ggtttccgcc ggaatcccgc accacatgtc cagttccacc
     2401 accgcgtcgt cggccgccgt cgtccccgga tccctcgccg cctggagcat cgcctgccgg
     2461 ttgccgagcc tgctggtcgc cgtcagcccg gtgctggtgg ggacggcact ggcgtgggag
     2521 cgaaccggcc agttcgacgc ggccacaatg ctgctcgcgc ttgcatgtgc cgtgctgctg
     2581 caggtgatat cgaacctgca gaacgacgtc ggctacacgg tccgcggtgc cgagcggcac
     2641 ggcaatcgca ccggactgcc acgcgccacc gccaacggct ggctcggcgt gcagcaagtc
     2701 agaatggcca tcgtcatctg ctgcggactg acaatgaccc tggggtcgcc gctcgtgctc
     2761 gctcatggct ggccggtgct cgcgatgggg ctgggttcgc tggtggccgc gctcgcctac
     2821 atgggcgggc cgcgtccggt cgcctacacg ccgtacggcg agttggtcgt gttcgtgttc
     2881 ttcggcctgg tcgcggtggc cggttccgac tacgtgcaga ccggcagtgt cggcacgacg
     2941 acatggctgg ccgggatcgc gctgggcgca ctcgcggcct cggccctggc cgtcaacaac
     3001 catcgcgatg tcgcgcatga ccgcagcgtc ggccgacgca ctttcgccgt cgtctttggc
     3061 gactcaacct cgcgggccat gtatgcactg agcgtgttcg gcgccttcgt cctgcttgtt
     3121 ccgatggcgt ggctgtcgag cccggccttg ctgacgccgc tgttgctggc gccggtagcg
     3181 ttgcgattgc agcgcgactt cgcccgcagc gcccccgggc tggcctacaa cgcaatcctg
     3241 gtacgtacct tcaagctcga actcggctac gcggccctgc tcgctgccgg cacggtggta
     3301 ggccggctcg ctgcctgaag ggtcgtccga tctcagtgcg ggatcagcgc gggctgcccg
     3361 ctccgactgc ggccagcgag gtcatcggcg tggctgccgg agtcggctgg gttgaattca
     3421 gcaatgcaat ctgcacgacg gcgatatccc atccggcctt gatgaccttg gtcgctgcac
     3481 gccgcttcat cgacatggag cgaacgaagt agaacctgcg cccgtccatg gacgccagtt
     3541 tcgccagcgc ccgcctcatc tgcgcgtcga tcacgccgac cggttcctcg gatgcagcca
     3601 cctcggcaca tatcactgcg gaatcgccgt cgcgtccgat cagatcgggc gccg
//
LOCUS       DATIRT010000279         3746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1616082, whole genome shotgun sequence.
ACCESSION   DATIRT010000279 DATIRT010000000
VERSION     DATIRT010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1616082"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VLN25_06330"
     CDS             complement(<1..289)
                     /locus_tag="VLN25_06330"
                     /inference="COORDINATES: protein motif:HMM:NF015573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HSQ10215.1"
                     /translation="MTITLNELWTGIAALATILLGVRINRWVPVLAAYSIPPAVTGGL
                     LIAILLAVIGGIAGVKVTFGDTLRNALLLIFFASLGLTAKFRRLSRGGGAVA"
     gene            complement(548..1705)
                     /locus_tag="VLN25_06335"
     CDS             complement(548..1705)
                     /locus_tag="VLN25_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HSQ10216.1"
                     /translation="MRDFSFNSVVAGFVAVLVGYTSSAVIVFEAARAAGATPAQTASW
                     MWALGLGMGLTTIVLSLRYRMPVLTAWSTPGAALLITGVGGLPMAEIVGAFVLTGLAI
                     VAIGLSGAFDRLMKRLPAALAAAMLAGVLLRFWLDAFGAMKSQFGLVFAMFATYLIGR
                     RVWPRFAVPVALAVGASIAGALGLFAELEWPTGLTRPEFVAPELSWRAAIGVALPLFV
                     VTMASQNVPGVATLQAHGYTPPVSPIITTTGVATTLLAPFGGYAFNLAAITAAICMGH
                     DAHHDPARRYTAAVSAGVFYVLIGLFGGAVAGLFAAFPRELVLAIAGLALLGTIGNGL
                     AAALKEESTRDAAVITFVATASGVSLLSIGSAFWGLVAGLLTLAVRGWRRR"
     gene            complement(1796..1933)
                     /locus_tag="VLN25_06340"
     CDS             complement(1796..1933)
                     /locus_tag="VLN25_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10217.1"
                     /translation="MTLELWPPAGPRVLVVEWSGRRPYVVYRRDGDWLDSVVNATRRL
                     K"
     gene            2579..3058
                     /locus_tag="VLN25_06345"
     CDS             2579..3058
                     /locus_tag="VLN25_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10218.1"
                     /translation="MSLAALSLSFVGFSAVVVTLRGALGGEISERHLRLVRLYIEGGL
                     LVTALALIPALLNLLHVSDSVIWPVASAAAAAIFSFLLVTQFRRRRAIEAGQFPPWVV
                     VVYAISLIAVVGLWLNVAGIPFRPNVGPYAVTLTWALCVFGFIFVRTIDIFLHRAHP"
     gene            3125..3586
                     /locus_tag="VLN25_06350"
     CDS             3125..3586
                     /locus_tag="VLN25_06350"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSQ10219.1"
                     /translation="MTAALVLRLATSDDARAVAEVLIRSRAEYLPFAPSAHSPDDVRA
                     WVANRLVPTGGVTVAEHQGAVVGVLAISRLDGVSWIDQMFVRPGWTGRGVGTQLLEHA
                     HRLLPRPIRLYTFQANTGARRFYERHGYRSIEFTDGQTNEEHCPDALYELS"
BASE COUNT          700 a         1266 c         1153 g          627 t
ORIGIN      
        1 atgccaccgc tccgccgcca cgcgacaggc gccggaactt ggcagtcagt ccgagcgacg
       61 cgaagaaaat cagcaatagc gcgttacgta acgtgtcgcc gaaagtcacc ttgacaccgg
      121 cgattccgcc gatgactgcg agcaggatcg caatcagcag gccacccgta acagccggtg
      181 gaatgctgta cgccgccagc accggcaccc accggttgat ccggacaccg agcagaatgg
      241 tcgccagtgc tgcgatgcca gtccacagtt cattcagcgt gattgtcatc gtctcaagcg
      301 cgtgacaagg gcaaaggcgt cattatcggc acagtcgtca gtgttcctag cgccgggtcg
      361 acgaaacgca acaactcgac tcacatcgga tgacgggcgg ctgtgaagtc gggcaaattg
      421 cggccgggaa tctcggattg cggcataagt cggcgcgact gcggctccta tcggtgggtt
      481 gccgtggcgg ccgctcggga agcggctgca gcagcggcga aggcgggggt tgcggtggag
      541 gcggcgctca gcgccgacgc cagccacgca ccgccagcgt cagcagcccc gccaccagac
      601 cccagaaggc cgatccaatc gaaagcagcg acacgcccga cgcggtcgcg acgaaggtga
      661 tcacggcagc atcgcgcgtc gattcctctt tcagagcggc cgccaggcca ttgccgatcg
      721 ttcccagcag cgccaggccc gcgatggcca gcaccagctc gcgcggaaac gcggcgaaca
      781 ggcccgcaac ggctccgccg aacagaccga tcagcacata gaacacaccg gcagacacgg
      841 ccgccgtgta gcgacgcgcg ggatcgtgat gcgcgtcatg ccccatgcag atcgccgcag
      901 tgatcgccgc gaggttgaat gcatagccgc cgaagggcgc cagcagcgtt gttgccacgc
      961 cggtcgtcgt gatgatcggc gacaccggtg gcgtgtatcc gtgcgcctgc agggtcgcca
     1021 cgccgggcac gttttgcgac gccatcgtga cgacgaacag tggcagcgcc acaccgatag
     1081 cggcccgcca ggacagttcc ggcgcaacga attccggccg agtgagtccc gtcggccact
     1141 cgagctctgc gaacagtcca agcgcgccgg cgatcgatgc accgacggcg agcgcgaccg
     1201 gcactgcaaa gcgcggccag acacgacgtc cgatcaggta ggtcgcgaac atcgcgaaca
     1261 cgaggccaaa ctggctcttc atcgcgccga atgcgtccag ccagaagcgc agcagtacac
     1321 cggccagcat cgccgccgcc agcgccgcgg gaagccgctt catcagccga tcaaacgcgc
     1381 cgctgaggcc aatcgccacg atcgcgagac cggtcagcac gaatgcaccg acgatctcgg
     1441 ccatcggcag gccgccgacg ccggtgatca gcaatgccgc acccggcgtc gaccaggcgg
     1501 tgagcaccgg catccggtaa cgcaacgaca gcacgatcgt cgtgagcccc atgccgaggc
     1561 cgagtgccca catccacgac gccgtctgcg ccggtgtcgc gcctgccgcg cgcgccgcct
     1621 cgaacacgat caccgccgaa ctggtgtaac ccaccaggac cgcgacgaag cctgccacga
     1681 ctgaattgaa cgaaaaatcg cgcacgccgc gagcgtatcg caggccctgc gcggcatggc
     1741 tcttagcgat tggtgcgtcg cccggcggat cgctatggcc tgatcaggcc cccgctcact
     1801 tcaatcggcg cgtagcgttg accacggagt cgagccagtc accgtcccgt cgatacacga
     1861 cgtacggccg gcgaccgctc cactcgacca cgagcacacg cggtccggca ggcggccaca
     1921 gttcgagagt cacccggcgc cctgcgtctt ggtgggctgc gtctcgtgcc gccgcgcggg
     1981 atatgaccac ctgcagatcg ttgagccgta agtgacgcac ctcgatctga tcctcgacgt
     2041 cccgaaacgc agtctccacc atgggccaaa gggtctgagc aagcaactgc tgcagtcctt
     2101 cgacctttac acggggccgt cgtgacggcg agacagcagc cgcggttgac cgagcactcc
     2161 ccacacgttg gcccggactg ccgtcccgtg ccggaggcaa ccttagcgtt gatcgcaact
     2221 ggagcaagcc ctttcgcacc gaacccatac gtatgccgca cgccgcattt tgatagccgg
     2281 aatttcatcg gttcaccgga gccttgccca tcatccgttg gggtggctgc ggtgcgaagc
     2341 agcaggcgac ttcgtcgcag gagagtcgcc gccagtgcgg caccccgacc ggcaactcac
     2401 ctctcaaagt tctcgccaac gccggacaat gcagacatca tgttcgagga agatgcatca
     2461 cctggaatcg caaacggcgc atcatgcacg gcggtcccgg ccattcgcgg gccgagaacc
     2521 acgtcctgcg caagcctcgg aggaaaccat cgtgacgtcc acagaaactg actttctgat
     2581 gagcctcgca gcactctcct tgtcgttcgt cggcttctcg gcggtggtgg tcactcttcg
     2641 aggtgcactc ggaggagaga tctccgagcg gcacctccgt ctggtgcggc tctatatcga
     2701 aggagggctc ctggtgaccg cgctggcatt gatccccgcg ctcctgaacc tcctccacgt
     2761 ctccgactcc gtcatctggc cggtcgccag tgcagccgca gcggcgatct tctcgtttct
     2821 cctggtgacc cagtttcgcc gtcgccgcgc catcgaggcg ggtcagtttc cgccatgggt
     2881 cgtcgtcgtt tatgcaattt ctctcatcgc tgtcgtcggt ctctggctca atgtggcagg
     2941 gattcccttt cggccgaatg tcggccccta cgcagtcacg ctcacctggg ccctttgcgt
     3001 attcggtttc atctttgtcc gcacgatcga catcttcctg caccgtgctc acccctgaag
     3061 ccgtcgacag ccctcgcggc ggtcgccatg gcaacgtctc tcggactgga cgattgctga
     3121 gtggatgacc gcggcccttg tattgcgcct ggccacatcg gacgacgctc gagcggtggc
     3181 cgaagtcctg atccggtcgc gtgccgagta ccttccgttc gcgccgtcgg cccactcgcc
     3241 cgacgacgtg cgggcctggg tggccaatcg attggtgccg acgggtggcg ttaccgttgc
     3301 tgagcatcaa ggcgcagtcg tcggcgtact ggccatctcc cgcctcgacg gcgtctcctg
     3361 gatcgatcag atgtttgtgc ggccgggatg gacgggccgg ggagtgggaa cccagttgct
     3421 ggagcatgcc catcggctgc tgccccgacc gattcgcctc tacacatttc aggcgaacac
     3481 gggagcacgc cgcttctatg agcggcacgg ctaccggtcg attgaattca cggacggcca
     3541 gaccaatgag gaacattgcc ccgacgcgct gtacgaactg agttgagcga aggatgctcg
     3601 ggctcaatgc tgtgggatgc ttctcgtagc ggcccatcca atttagagtt gccatcgtcg
     3661 tcgccgcaat cgaggtcaga gaatgcaggg cagtcagcag gctcatgagc atggtcggcg
     3721 cgggctgctc agccacttta ttcagc
//
LOCUS       DATIRT010000280         4568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1125325, whole genome shotgun sequence.
ACCESSION   DATIRT010000280 DATIRT010000000
VERSION     DATIRT010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125325"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /gene="rocF"
                     /locus_tag="VLN25_06355"
     CDS             <1..806
                     /gene="rocF"
                     /locus_tag="VLN25_06355"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HSQ10220.1"
                     /translation="GLEVIDHGNLNGPPNPWLPPVDGYRHLDEVVAWNRLVHEAVGAE
                     LAGGRLPILLGGDHCLAIGSISAVARHCSERGKRLRVLWLDAHADFNTHALTPSGNVH
                     GMPVACLCGHGPRPLIEIGGTVPAIDPRWIRQIGIRSVDAGEKHFVHEAGLEVFDMRF
                     IDELGMRHAMELALATMDPGTHLHVSFDVDFLDPEIAPGVGTTVSGGPTYREAQLCME
                     MIADSGRLGSLDVMELNPALDVRNKTALLTVDLIESLFGKSTLMRQPSR"
     gene            996..1601
                     /locus_tag="VLN25_06360"
     CDS             996..1601
                     /locus_tag="VLN25_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10221.1"
                     /translation="MKKLNIAAVAILAVLAAPALAQAPSGGVVMKESQPGKVAVAEAI
                     RVSASVEAIDKASRMVTLKGPEGNVFAVQAGPDVKNFDRIKVGDFVVVRYVEALTLEL
                     KKGGGGIRERVESEGGAAAKPGERPAGAATRQVTVVADVIAVDAKKQSVRLRGPQRTI
                     DLKVRDPEQFKLIKVGDQIEARFTEALAVSVEPAPKPAAKK"
     gene            2002..4050
                     /locus_tag="VLN25_06365"
     CDS             2002..4050
                     /locus_tag="VLN25_06365"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HSQ10222.1"
                     /translation="MFRVRQRLFVVAVVSVFALGSSQTARAATSQGEREGTCANAATL
                     FPDTNPGPGSLKRIAVPEPTDLGVYVRDRAAAIVLGKALFWDMQVGSDGIVACATCHF
                     RAGADPRSINQLNPGGQDNPRQVVDLGINRRLQPADFPLRQLADPTDRRSAVLRDSDD
                     VVSSAGVKLSRFVGVRPGADQDIVQTVADPVFNLAGLNTRRAEPRNTPTVINAAYNRR
                     QFWDARASNLFNGVSPFGAGDADARVLIATSRGKLRPIKIRIDNAGLASQAVGPPLSD
                     REMGSLDRKFRDIGKRLASARALRKQRVAADDSVLGRHARTLADAHRDEFSDRGLDTT
                     YRELVEAAFHARWWQADEIVVVRPDGRIDFEATPRRPLRSHEYTMLEYNFSLFFGLAV
                     QLYEMTLISDDAPVDQHFEGHPRAMTPKQLRGMEVFTGDAACAACHSGAETTDNSVRI
                     LMGTMVNGVKQPAEVVERMFNGNCEVVAYDQGVYNLGVRPFEEDIGIGARDPFGNPLA
                     FISLLTSPPQSIPSKELLTLPIPNIANPPIAVGERALTTGAFKVPNLRNLALTTPYFH
                     NGGQATIRQAVEFYNRGGDFREHNTAFIDFEVGKLNLTEQQIDDLVEFLTTALTDPRV
                     VRQSAPFDHPQLFVPNGHETRGGAPVVDPDGTVRDRMLEIPAVGRRGGPLPAGFLEGN
                     "
     gene            complement(4160..4568)
                     /locus_tag="VLN25_06370"
                     /pseudo
     CDS             complement(4160..4568)
                     /locus_tag="VLN25_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111903.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
BASE COUNT          786 a         1475 c         1551 g          756 t
ORIGIN      
        1 atggcctcga ggtcatcgac cacgggaacc tcaacgggcc acccaatccg tggttgccgc
       61 cggtcgacgg ctatcggcat ctcgatgaag tggtcgcgtg gaaccggctc gtgcatgaag
      121 cggtcggagc cgagctggcg ggcggccgcc tgccgatcct gctcggaggc gaccactgcc
      181 ttgcaatcgg ctcgatcagc gcggtcgcac gccactgcag cgagcggggc aagcgcctcc
      241 gcgttctctg gctcgatgcc cacgcggact tcaacaccca tgcattgacg ccgagcggca
      301 atgtgcacgg catgccggtg gcgtgcctgt gcggccacgg cccgcgcccg ctgatcgaga
      361 tcggaggcac cgtgccggcg atcgacccgc gctggatccg gcagatcggc atccgcagtg
      421 tcgatgcggg tgagaagcac ttcgtgcacg aggccggact cgaagttttt gacatgcgtt
      481 tcatcgatga acttggcatg cggcatgcga tggaactggc gctggcgacg atggatccgg
      541 gcacgcatct gcatgtcagc ttcgacgtcg attttctcga tccggagatc gcgcccggcg
      601 tcggcaccac ggtgtcggga gggccgacct atcgcgaggc gcagttgtgc atggagatga
      661 tcgccgacag cgggcggctg ggctccctcg acgtgatgga gctgaaccct gcgctcgacg
      721 tccgcaacaa gacggcgctg ctgacggtcg acctgatcga gagcctgttc ggcaagagca
      781 cgctgatgcg tcagccttcg cgctgaggcg gcgtatcggt agcgacgtcg ggtacggcgc
      841 gctggcccgg gtgcaggatt gacccgccgg accatcgcgc gcgttggttt acgatgcgat
      901 ccccgcgctg ggccctgagc agtccggtcc gcggcggcag tgcagggagg tgccggcgtg
      961 accggcgttt gatgaatcct gatcggaaag taaacatgaa aaaattgaac attgcggccg
     1021 tcgcgatcct ggcggtgctg gctgcgccgg ccttggcgca ggccccgagt ggcggcgtcg
     1081 tgatgaagga gagccagcct ggcaaggttg cggtcgccga ggcgattcgc gtatcggcca
     1141 gcgtcgaggc tatcgacaag gcaagccgca tggtcacgct gaagggcccc gaaggcaatg
     1201 tgttcgctgt acaggcagga cccgacgtca agaatttcga ccggatcaag gtcggcgact
     1261 tcgtcgtggt ccgctatgtc gaagcgctga cgctggaact gaagaagggc gggggcggca
     1321 tccgcgagcg tgtcgaaagt gaaggcggcg ctgccgctaa accgggcgag cgccccgccg
     1381 gcgccgcgac ccgacaggta acggtcgtcg ccgatgtcat cgcggtcgat gccaagaagc
     1441 agagcgtgcg gctgcggggt ccgcagcgga cgattgacct caaggtccgc gatcccgagc
     1501 agttcaagct gatcaaggtc ggcgaccaga tcgaagcgcg cttcaccgaa gcgctcgccg
     1561 tctcggtcga accggcaccg aagcccgccg cgaagaagta gtctgaaagt ccggcggcgg
     1621 caggcctgcc ctgccgccgc tgcgccgtcg cgcacagcga cccgccactc gcgggggcat
     1681 acgcgcgcgt cgtcgtgcgc cggcagcgcg attccaggtc cggcgcagca gcgtcgtccc
     1741 gctcccagtc acagtcggcc cccgtgcggc gtcggctcag ccgaacggga tatgccgttt
     1801 ggcaaatggc gaagcctggc gttcctcaac agaatttgcg cagcgaagcg gatgtgtcgg
     1861 tgcggcaaac gggccgatat ctcaccgctc gcgagcgacg cgggccgttc ccgtccactt
     1921 ggctgactcc gggccggggc gaggcgggag aggtgtcacc tgcgttccag ctggccacac
     1981 agcaggaaag gcaggtgcac catgttcagg gtcaggcagc gtctcttcgt ggtcgcggtg
     2041 gtgtcggttt ttgcgctcgg cagcagtcag accgcccgcg cggcgacgtc ccagggcgaa
     2101 cgcgaaggaa cctgcgccaa tgcagcgacg ttgttccccg acaccaatcc gggtccgggt
     2161 tcgctcaagc gcatcgccgt gcccgagcca accgatctgg gcgtctacgt gcgcgatcgc
     2221 gcggcggcca tcgtgctcgg aaaagcgctc ttctgggaca tgcaggtcgg cagtgacggc
     2281 atcgtcgcgt gcgcgacctg ccactttcgc gccggcgctg atccgcgctc gatcaaccag
     2341 ctcaatcccg gtggtcagga caatccgcgc caggtcgtcg acctcggcat taaccgtcgg
     2401 ctgcagccgg cggacttccc gctgcgtcag ttggccgatc cgacggaccg ccgttcggcc
     2461 gtcctgcgcg acagcgacga cgtcgtttcc tccgccggcg tgaagctcag tcgcttcgtc
     2521 ggcgtccggc cgggcgcgga ccaggacatc gtgcagaccg tggccgaccc ggtcttcaat
     2581 cttgccgggc tcaacacgcg ccgcgcggaa ccgcgcaaca cgccgactgt gatcaatgcc
     2641 gcgtacaacc ggcgccagtt ctgggatgca cgggcatcga atctgttcaa cggcgtgagc
     2701 ccgttcggtg ctggcgatgc cgacgcacgt gtgctcatcg ccacatcccg tggaaagctt
     2761 cgcccgatca aaatccgcat cgacaatgcg ggcctcgcgt cgcaagcggt gggcccaccg
     2821 ctcagcgatc gtgagatggg atcgctcgac cgcaagttcc gcgacatcgg caagcggctc
     2881 gcatccgcgc gggcgctgcg caagcagcga gtggccgcgg acgacagtgt cctcgggcga
     2941 catgcccgca ccttggccga cgcgcaccgt gacgagttca gcgatcgtgg gctcgacacc
     3001 acgtaccgcg aattggtcga ggctgcgttt cacgcgcgct ggtggcaggc cgacgagatc
     3061 gtcgtggtgc gtcccgacgg acgcatcgac tttgaggcga caccgcgtcg gccgctgcgt
     3121 tcccacgagt acacgatgct ggagtacaac ttttccctgt tcttcgggct ggccgttcaa
     3181 ctttacgaga tgacgttgat ctcggacgat gcgccggttg accagcactt tgaaggtcat
     3241 ccgcgggcca tgacgccaaa gcagttgcgc ggcatggaag tgttcaccgg cgatgcggcc
     3301 tgcgcggcgt gccattcggg cgccgagacg acagacaact ccgtgcgcat cctgatggga
     3361 acgatggtca acggcgtgaa gcagcccgcc gaagtggtcg agcgcatgtt caacggcaac
     3421 tgcgaagtcg tcgcctacga ccagggtgtc tacaaccttg gcgttcgacc gttcgaggag
     3481 gacatcggca tcggagccag ggatccgttc ggcaatccgc tcgcattcat cagcctgctg
     3541 acgtcgccgc cgcagtcgat tccgagcaag gaactgctga cgctgccgat tccgaatatc
     3601 gccaatccgc cgatcgctgt gggcgaacgc gcgctgacga ccggcgcgtt caaggtgccc
     3661 aatttgcgca acctggccct gaccacgccc tacttccaca acggcgggca ggcgacgatc
     3721 cggcaggccg ttgagttcta caaccgcggc ggcgattttc gtgaacacaa caccgcgttc
     3781 atcgacttcg aggtcggcaa actcaacctg acggagcagc agatcgacga tctggtcgaa
     3841 ttcctgacga cggcgctgac cgatcctcgg gtcgtgcggc agagtgcgcc attcgatcac
     3901 ccgcagcttt tcgttcccaa cgggcacgaa acgcgcggtg gggcaccggt ggtcgacccg
     3961 gatggcaccg tgcgggatcg gatgctggag ataccggcgg tcggtcgccg gggcgggccg
     4021 ctgccggcag gcttcctcga agggaactga aacgaagggc cgtacgccag ccgcaaggcg
     4081 cgtgcgtgcg ccgacgcggc cgggtgttgc cacgacgcac tcggcatccg gcgcaggtac
     4141 tcgccggccg atcagagcgc tatggcatca gctgaccgcc gttgacttcg aggatctgcc
     4201 cggtgacata gccgctcagc gcatcggatg cgaggtacag gtaggcgccg acgcagtcat
     4261 cgacgctgcc cagccggccc atcggcactg ccgcgcgaaa agactacatc tgggctgcgc
     4321 tggtgtgacg cgcctgcagc ggcgtgacga tgaccccggg cgcgacgccg ttgacgcgga
     4381 tgttttcctg cgccagttcc ttcgccaggc cgcgagtgaa ggtgctcacg aacgccttgg
     4441 aactcgcgta caggaccgag cccgggccgc cgccgttgcg tgccgcgatc gacgtcgtat
     4501 tgatgatgtt gccgtgtccc tgtgaacgaa agtgggggat cgcggcccga ctggtcgtga
     4561 cgaccgag
//
LOCUS       DATIRT010000281         6059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1321562, whole genome shotgun sequence.
ACCESSION   DATIRT010000281 DATIRT010000000
VERSION     DATIRT010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6059
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321562"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..909
                     /locus_tag="VLN25_06375"
     CDS             28..909
                     /locus_tag="VLN25_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSQ10223.1"
                     /translation="MMKPLVPLFASLTLGVVAGEVGAQASPAALSLQVYNADAGSFHV
                     NAVLVSGKTDAVLIDTGFTRADAYRIAAMVLDSRKTLKTIYISQADPDFYFGAGVLKA
                     AFPDATLIATGPTVKKLKATLPVKLQVWGPRLGANAPTDVPVPEVLSGNTILLEGQTL
                     EIRGLDDSLPQRSYVWIPSLKAIVGGVNVFAGLHAWTADTQTAQERGDWNRKLGTMAA
                     LQPAIVVQGHMLTGQSQDASQLAYTQSYLTRFELELAKAPNSAALIEAMKSAYPSAGL
                     MVALEIGAKVNKGEMKW"
     gene            complement(1094..2608)
                     /locus_tag="VLN25_06380"
     CDS             complement(1094..2608)
                     /locus_tag="VLN25_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HSQ10224.1"
                     /translation="MKKPRWKQFAAVAGTAALITLLASCGKQDAKVDKAMDKAAAEVQ
                     AKAGAVVKAAESAAKEAESLALATEAYVYAYPLVTMEMTRRVMTNVEKPEGTRSPVGQ
                     FVRMREYPSAKFRDVTAPNADTLYTTTWLDVSKEPWILSIPDMKGRYFLFPMLDGWTN
                     VFQVPGKRTTGTKSQKIAITGPGWTGTLPAGVTEYKSPTGMVWILGRIYSTGTPQDYK
                     EVHALQDQVSVVPLSSYGKPFKAEPTKVDAAIDMKTAVREQVNAMDAAAYFKLFANLL
                     KANPPAAEDAPMVAKLAKIGIVPGQEFDAAKLEPAVANGMAGAPKHAQDLIMGWMKAG
                     IAAGDMKLENGWIFTTKTGVYGTNYLQRALITAIGLGANRPQDAVYPTSEGPDILKKY
                     SGEKTYVMRFAKGELPPVDGFWSLTMYDKDYFFVDNPLNRYNLSQRNKLKANPDGSID
                     LYIQHASPGKDKESNWLPAPKDPFILMMRMYWPKEKPPSILDGTWKVPEVKEVS"
     gene            complement(2840..3946)
                     /locus_tag="VLN25_06385"
     CDS             complement(2840..3946)
                     /locus_tag="VLN25_06385"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HSQ10225.1"
                     /translation="MSRRLRHFIGMLVPIVTTVATAPGALAQADVIRAKEITRLSPKA
                     SVVLDRNCPDIVQPYTLADNTASLALFGAKEAISDVGARLLANAGSILAGNRPTLAPS
                     TSKLTASTKLAAKQLNWLPMTAEVAYGERLHQDEVAILERDSKLGQRYYPIADRLLEE
                     VLASVGQPHDYQFKLFILKNSSRNAAARPGGFLYLDQGLVDDPAQLPKAYFAVAHEVA
                     HVLQRHETKELQSNVVDSISSKDDLMKVVTGSQGNPNLILSYVQVEKNRYTRHHIDQE
                     LQSDSCAVRLLSRALPDPQALAASINAFVQDLPQPEQFTGPKASSTDEGKLAKSVHDI
                     VDGPVKRHPNNEERLQNLRSITAEIGAGAPPQRP"
     gene            complement(3971..4408)
                     /locus_tag="VLN25_06390"
     CDS             complement(3971..4408)
                     /locus_tag="VLN25_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10226.1"
                     /translation="MKRLVMGVTLLVASWSWPPSAGAQNCRGLLDIPATASSALTAVI
                     KSLSDTNAACGSVKVVLSRVVSREKTGGRKLEPDKPLDLKKAQVNLEAALRDPEIKAR
                     VEKARAEIKDESVLLAFEAAVFDEEGFYDARDLKIQQLQQRLN"
     gene            complement(4423..5916)
                     /locus_tag="VLN25_06395"
     CDS             complement(4423..5916)
                     /locus_tag="VLN25_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10227.1"
                     /translation="MRDDWPATVFVAALFAILDSHVGWLDAINGHAFVAIGNLAGIPQ
                     ETGAAKAKALVVVIDQAAAESRYLDRSPLDRCQLLADLGAVYSAMNRRNQQRDVLRAK
                     APASSAQLLDLLVVDLDLSPAPWLATVEGQKSAEAQCERDLHRMIAQANSQWRIRTVL
                     MNPFESVDSGVKTAKAAWKSNLEGAAVTFGRAVLPIEYGLVIKQYCAPDAMAVTAYDL
                     WVQRKRANNEAHEVNECIEAARDPARNPKGASQHIDPRRYLSGVVPLAASAQPGFNGQ
                     LDRALGTDASPNAAAGGAEFQAVFFGAAYGEGDTFVTPLGELYGVEIHAASFLSFLDP
                     LSADNHLAELLADIVFGFVFGFVIAFFWEQYFRFRLSDDSDRRLIAPVVLGGLGVSVA
                     LVAFVLTIVSWLLLARFGIWSSPIPMAIGMLIESFVSGSVAQGIREADSLKGGGRPPR
                     QTFRESARKFFGGSIVSLLRKGRPLSATLLTLRLGIWVSVVAVAFMR"
BASE COUNT         1067 a         1902 c         1897 g         1193 t
ORIGIN      
        1 gtcactcacc ccactgcgag ccattcaatg atgaaacctc tcgttccgct atttgccagc
       61 ctgacactcg gtgtcgtcgc aggcgaggtc ggcgcccagg cgagtcccgc cgcgctgtcc
      121 cttcaggtct acaacgccga cgccggcagc tttcacgtca acgcggttct ggtttccggc
      181 aagacggacg cagtgctcat cgataccggg ttcacccggg ccgatgcgta tcggatcgct
      241 gcgatggtgc ttgacagtcg caagacgctg aagacgatct acatcagcca ggccgacccg
      301 gacttctact tcggcgcggg tgttctgaag gccgcgtttc ccgacgcgac attgatcgcg
      361 accggaccca cagtgaagaa gctcaaggcg acgctgcctg tgaagctgca ggtgtggggg
      421 ccgcggctgg gcgccaatgc gccgaccgac gttccggtgc ccgaggtcct gagcggcaac
      481 acgatattgc tggaaggtca gacgcttgaa atcaggggcc tcgatgattc acttccgcag
      541 cgcagctacg tctggatccc atcgctgaag gccattgtcg gcggagtcaa tgtctttgcc
      601 gggctgcacg cgtggacggc ggacactcag accgcgcagg agcggggcga ctggaaccgg
      661 aaactgggca cgatggctgc gcttcagccg gcgatcgttg ttcagggcca catgctgaca
      721 gggcagagtc aggacgcttc gcaactcgcc tacacccagt cttacctgac ccgctttgaa
      781 ctggaactgg cgaaagcgcc gaactcggcc gcactgatcg aggcgatgaa gtcggcctat
      841 ccctccgcgg gcctgatggt ggcgctcgag atcggcgcca aggtcaacaa gggcgaaatg
      901 aagtggtgat gcaaacgaac tgagcgagca tctgccggct gaatggacgc tggcgatgcc
      961 gttcacgctt gtccgctatg ggtgcgatcg cgtcgaagcg cgcgatggct gcatttggcg
     1021 ggcaggagct tttcaattcg gcaccgcctg cagcgttcgg cggcaagcgc cgccgaacgt
     1081 gccgacttct agactacgac acttccttca cttccggaac cttccacgtt ccatcgagaa
     1141 tcgagggcgg cttctctttc ggccagtaca tgcgcatcat caggatgaac gggtccttcg
     1201 gggccggcag ccagttcgat tccttgtcct tgccgggcga cgcgtgctgg atgtacaagt
     1261 cgatcgagcc gtccgggttg gccttcagct tgttgcgctg gctcaggttg tagcggttga
     1321 gcgggttgtc gacgaagaag taatccttgt cgtacatcgt cagtgaccag aacccgtcca
     1381 ccgggggcag ttcacccttt gcgaaccgca tcacgtacgt cttctcgccg ctgtacttct
     1441 tcaggatgtc cggtccctcg gaggtcggat acacggcgtc ctgaggccgg ttggcgccca
     1501 ggccaatcgc ggtgatgagc gcgcgctgca ggtaattggt gccgtaaaca ccggtcttgg
     1561 tcgtgaagat ccacccgttc tcgagcttca tgtcaccggc agcgatgcct gccttcatcc
     1621 accccatgat caggtcctgc gcgtgcttgg gcgcaccggc cattccattg gcgacggcgg
     1681 gctcgagctt ggctgcgtcg aactcctggc cggggacgat gccgatcttt gcgagcttcg
     1741 ccaccatcgg cgcatcttca gccgccggcg ggttcgcttt caacaagttg gcgaaaagct
     1801 tgaagtacgc agcagcgtcc atggcgttga cctgctcacg gacagcggtc ttcatgtcga
     1861 tggccgcatc gaccttggtc ggctccgcct tgaagggctt gccgtacgag gacagaggca
     1921 ctaccgacac ctggtcctgc agcgcatgca cttccttgta gtcctgcggc gtaccggtgc
     1981 tgtaaatgcg accgaggatc cagaccatgc cggtcgggga cttgtactcg gtaacgcccg
     2041 ccggcagtgt tccggtccag cccggaccgg taatggcgat cttctgcgac ttcgtgccgg
     2101 ttgtgcgctt gcccggcacc tggaatacgt tggtccagcc gtcgagcatg ggaaacagga
     2161 agtagcggcc tttcatgtcc ggaatgctga ggatccaggg ctccttcgag acgtcgagcc
     2221 aggtggtcgt gtagagcgtg tcggcattcg gcgccgtgac atcccggaac ttcgcgctgg
     2281 ggtactcgcg catgcggacg aactggccca ccggggaacg cgtgccttcc ggcttctcga
     2341 cattggtcat cacgcgccgg gtcatttcca tcgtgaccag cgggtaggcg tacacgtagg
     2401 cttcggtcgc gagcgccagc gattccgctt ccttggcggc cgactccgca gcctttacaa
     2461 ctgcgccggc ctttgcctgg acttcggccg ccgccttatc catggccttg tccacctttg
     2521 cgtcctgctt gccgcacgac gcgagcagtg tgatcagcgc cgccgttccg gcgacggcgg
     2581 caaattgctt ccaacgcggt ttcttcatct tcttatgcct cgggctgtgg tggttcgaac
     2641 gcgacgtcta tcccgtgccg ccgcgcagga gtcatgtgtc actcgcgcga attcaacagg
     2701 gggaaagttc gttccgtaaa gggagttcaa gggaggccgc tgcgcgagcc ggggatgtta
     2761 gcggacgatg cgaatcgtcg ggattgcccg cgccagttcg atcgcgatgt gttgcctcga
     2821 tgcgaccttg agcagtctgc tagggtcgct gcggtggggc ccctgcaccg atttcggcgg
     2881 tgatcgagcg caagttctgc agccgctcct cgttgttcgg atggcgcttg accgggccat
     2941 cgacgatgtc gtgcaccgac tttgccagct tgccctcgtc ggtcgaggac gccttcggcc
     3001 cggtgaactg ttccggctgg ggcaggtcct gaacaaaggc gttgatcgac gcggcgagcg
     3061 cctgcgggtc gggcagcgcg cgactcagca gccgcacagc acacgagtcc gactgcaatt
     3121 cctggtcgat gtggtgccgg gtgtatcgat tcttttccac ctgaacgtag gacaggatca
     3181 ggttcgggtt gccctgggaa ccggtcacca ctttcatcag atcgtccttg gatgaaatcg
     3241 agtcgacgac gttgctttgc agctccttgg tctcatgccg ctgcagcacg tgcgccactt
     3301 cgtgcgcaac ggcgaagtac gccttcggca gctgcgccgg atcgtcgacc agaccctggt
     3361 ccaggtagag aaatccgccg gggcgcgccg cggcgttgcg cgatgaattc ttgagaatga
     3421 aaagtttgaa ctggtagtcg tgcggctgcc cgacgctggc aaggacttcc tcgagcagcc
     3481 ggtcggcaat cgggtagtag cgctggccca gcttgctgtc gcgctcgaga atggcgactt
     3541 cgtcctggtg caggcgttcg ccataggcca cttcggcggt catcggcagc cagttgagct
     3601 gcttcgctgc cagcttggtc gaggcggtga gcttgctggt cgaaggggcg agtgtgggcc
     3661 ggttgcccgc caggatgctg ccggcgttgg cgagcaggcg ggcgccgaca tccgatatcg
     3721 cttccttcgc gccgaacagc gccagtgaag cggtgttgtc ggccagggtg tagggctgga
     3781 cgatgtcggg acagttcctg tcgagcacca ccgaggcctt cggcgacagc ctggtgattt
     3841 ccttggcccg gatgacatcg gcctgcgcca gcgcacccgg ggccgtggcc actgtcgtca
     3901 cgatcggcac cagcatgccg atgaaatggc gtaagcgtct cgacatcgtc tgtccgtgag
     3961 cgcgtccgac tcagttgagt cgttgctgca actgctggat cttcaggtcg cgcgcgtcgt
     4021 agaaaccctc ttcatcgaat accgcggcct caaacgcgag cagcacgctc tcgtccttga
     4081 tctccgcgcg cgccttctcg acgcgtgcct tgatctccgg atccctgagg gcagcctcca
     4141 gattgacctg cgccttcttc agatccaggg gcttgtccgg ctcgagcttg cgaccgcccg
     4201 tcttctcacg gctgacgacg cggctcagca cgaccttgac cgaaccgcag gcggcgttgg
     4261 tatcggaaag actcttgatg accgccgtga gtgcggacga tgccgtcgcc ggaatgtcca
     4321 gcaggcctcg gcaattctga gcgccggccg agggcggcca cgaccacgac gccacgagca
     4381 atgtcacccc catgaccagc cgcttcatcc ccgcctctgc tcctagcgca tgaacgccac
     4441 cgcgacgact gacacccaaa ttcccagccg taatgtcagt agcgtcgctg acagtggtct
     4501 tcccttccgc aacagactga ctatagaccc gccgaaaaac ttcctggcac tttcccggaa
     4561 ggtctggcgc ggtggtcggc cgcccccctt gaggctgtcg gcttcgcgaa tcccctgggc
     4621 taccgagcct gacacgaagc tttcgatcag catgccgatt gccatcggaa tgggcgacga
     4681 ccagatgccg aagcgggcaa gcagcaacca cgagacgatg gtcagcacaa aggcgacgag
     4741 cgccacggag acgcccagac cgcccagcac gaccggtgcg atcaggcggc ggtcggagtc
     4801 atcgctcaac ctaaacctga agtactgctc ccagaagaag gcaatcacaa aaccaaagac
     4861 aaaaccaaag acgatgtcgg ccagcagttc ggccaggtgg ttgtcggcgc tcagtggatc
     4921 gaggaacgac aggaaactcg ccgcatggat ctcgacgccg tagagctcgc cgagcggcgt
     4981 gacgaaggta tcgccctcgc cgtatgcggc gccgaagaac accgcctgaa actcggcccc
     5041 gccagcggct gcgttcggcg acgcgtcggt tccgagcgcg cgatcgagct ggccattgaa
     5101 gccgggctgc gcgctcgcgg cgagcgggac cacgcctgaa aggtagcgcc gtggatcgat
     5161 atgctgggag gcgcctttgg gattccgtgc tggatccctc gcagcctcaa tgcactcgtt
     5221 gacttcgtgc gcctcgttgt tcgcgcgctt gcgctgaacc cacaggtcat acgccgtcac
     5281 cgccatcgcg tccggcgcac agtattgctt gatcaccagc ccgtactcga tcgggaggac
     5341 ggcgcgcccg aaggtcacgg ccgcgccttc caggttgctt ttccaggccg ccttcgctgt
     5401 tttgacgccg gaatcgactg actcgaatgg attcatcagc actgtgcgga tgcgccattg
     5461 actgttcgct tgtgcaatca tccggtgcag atcgcgttcg cactgggctt ccgccgactt
     5521 ctgtccctcg acggtcgcga gccacggtgc gggtgacaag tcgagatcaa ccaccagcag
     5581 gtcgaggagc tgggcggatg acgccggtgc ctttgcgcgc aatacatcgc gctgttgatt
     5641 gcgtcggttc atcgctgaat acactgctcc gagatcggcg agcagctggc agcggtcgag
     5701 cggtgaacgg tccaggtatc tcgattcggc agcggcctgg tcgatcacca cgaccagcgc
     5761 ctttgctttc gccgcgccag tttcctgcgg tatcccggca agattgccga tggcaacgaa
     5821 cgcatgaccg ttgatcgcgt ccagccaccc gacatggctg tccaggatcg caaagagcgc
     5881 cgccacaaaa acagtggccg gccagtcatc gcgcatgtgc tgaataaagt ggctgagcag
     5941 cccgcgccga ccatgctcat gagcctgctg actgccctgc attctctgac ctcgattgcg
     6001 gcgacgacga tggcaactct aaattggatg ggccgctacg agaagcatcc cacagcatt
//
LOCUS       DATIRT010000282         1655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1321639, whole genome shotgun sequence.
ACCESSION   DATIRT010000282 DATIRT010000000
VERSION     DATIRT010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321639"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VLN25_06400"
     CDS             complement(<1..195)
                     /locus_tag="VLN25_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10228.1"
                     /translation="MRVVRRVGWGGLTLALLVLTGVLWYRQASQPIHEGTVRVDGIAG
                     RIEVRRDEQGIPTIVASSEHD"
     gene            complement(364..1179)
                     /locus_tag="VLN25_06405"
     CDS             complement(364..1179)
                     /locus_tag="VLN25_06405"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel transporter"
                     /protein_id="HSQ10229.1"
                     /translation="MHELPTEWTALCALVFLLGLKHGFDADHLATIDGLTRYNARNGR
                     RFGRYCGALFSLGHGAVVLIIAAAVGLASERWNAPEWLEAFGAWTSILFLTLIGVVNL
                     HAVMSAAPGQVVAPVGLKGRLLGRLNRASHPATVALVGALFALSFDTVSQSAMFALTA
                     AQFGGVTHALVLGLLFVFGMLVTDGINGLWISRLIARADQLACIASRVMSLAVAGVSL
                     LVAAFGAAKFAMPAVENWSDGKELMFGSAVVLLILLAFVAGQMLARRPVVETA"
     gene            complement(1277..>1655)
                     /gene="nikR"
                     /locus_tag="VLN25_06410"
     CDS             complement(1277..>1655)
                     /gene="nikR"
                     /locus_tag="VLN25_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HSQ10230.1"
                     /translation="YSNRSEAVRDILRAHLDRLREHADAEAHCVANLSYVYNHHERDL
                     AERLTSLQHDHHDLTISTMHAHLDHDHCIESVMLKGRSRDVRKFADAISAERGVRHGQ
                     LNLVTVEVGHPSRHVHSHLTPNH"
BASE COUNT          354 a          513 c          508 g          280 t
ORIGIN      
        1 gtcgtgctcg ctgctggcga caatcgtcgg aatgccctgc tcgtcgcgcc gaacttcaat
       61 tcggccggcg atcccgtcca ctcgcacggt gccctcgtga atcggctggc tggcctgccg
      121 ataccaaagc acacccgtga gtacgaggag agccaacgtc agaccgcccc acccgacccg
      181 ccgcactacg cgcaccgcga ctccaatgaa gtcaaaatgt gcgaattgtg cgtgaaagaa
      241 gcgtcgaggt ctgcctcgag tggatcggct cagcgcaacc aggccgaagc gacgtggacc
      301 gatgcaaccc tgccgttgct tgcatccgac cctgcgtaac tgcgcagggc aaacggaatt
      361 cgtctaagca gtctcgacga cgggacgacg agccagcatc tggccagcca cgaatgccag
      421 caaaatgaga agcacgacgg ccgaaccaaa catcagttcc ttgccgtcac tccagttttc
      481 gacggccggc attgcgaact tggcggctcc aaaggctgcg acgagcagac tgacgccggc
      541 cacggcgaga ctcatcacgc gggaggcaat gcaggcaagt tggtccgcgc gggcgatgag
      601 gcgcgagatc cacaatccgt tgatgccgtc cgtcacgagc atcccgaaca cgaacagcag
      661 gccaagcacg agcgcgtgcg tgaccccgcc gaactgtgcc gcggtaaggg cgaacatagc
      721 ggactggctg acggtatcga aggaaagggc aaagagggcg ccgacgagcg cgaccgtggc
      781 gggatggctc gcccgattga ggcgaccgag cagtctgcct ttaagtccca ccggagcaac
      841 gacctgtccc ggtgcagcgc tcatgaccgc gtgcagattt accacgccaa tcaaggtaag
      901 gaacaggatc gaagtccacg ccccgaacgc ttccagccat tcgggagcat tccaccgttc
      961 actggccagg ccgactgctg ccgcaatgat caatacgact gcgccatggc ccagcgagaa
     1021 cagggcccca cagtaacgtc cgaaccgccg gccattgcga gcgttgtacc gggtcaggcc
     1081 gtcgatcgtc gccaggtgat cagcgtcgaa tccatgcttg aggccgagca ggaaaacgag
     1141 tgcgcacaag gcagtccatt ccgttggcaa ttcatgcatg gcgatctccg cgacaatgta
     1201 tgaactctaa gccaactcgt cttacccgtg ccttgcgccg gatcaaggga cgatccgccc
     1261 agattcaact cgcttgctag tgattggggg tcaggtggga atgaacgtgc cgggacggat
     1321 gcccgacctc gaccgtcacg aggttgagct gcccatggcg cacaccgcgc tccgctgaaa
     1381 tggcgtcggc aaatttgcgc acatcccttg aacgaccctt gagcatcaca ctttcgatgc
     1441 aatggtcgtg gtcgaggtgc gcgtgcatgg tcgagatcgt cagatcgtgg tggtcgtgct
     1501 gcagcgacgt cagacgctcg gccaggtcgc gctcgtggtg gttgtagacg taggagaggt
     1561 ttgcgacgca gtgcgcttcg gcatctgcgt gttcacgaag ccggtcaagg tgtgcccgca
     1621 gtatgtcgcg caccgcctcg gatcgattgc tgtaa
//
LOCUS       DATIRT010000283        11602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1321648, whole genome shotgun sequence.
ACCESSION   DATIRT010000283 DATIRT010000000
VERSION     DATIRT010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11602
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321648"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VLN25_06415"
     CDS             complement(<1..686)
                     /locus_tag="VLN25_06415"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416731.1"
                     /note="part of NADH-ubiquinone oxidoreductase complex I;
                     shuttles electrons from NADH, via FMN and iron-sulfur
                     (Fe-S) centers, to quinones in the respiratory chain; NuoF
                     is part of the soluble NADH dehydrogenase fragment, which
                     represents the electron input part of NADH dehydrogenase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HSQ10231.1"
                     /translation="MILAGLDGTNWRLKDYMQRGGYAALRKILTERMAPDAVIAELKK
                     SALRGRGGAGFPTGLKWSFMPRQFPGQKYVVCNSDEGEPGTFKDRDILRYNPHIVIEG
                     MAIAAYAMGVSVGYNYIHGEIWTDYERFEESLDEARAAGYLGRGILGSSFSFDLHASH
                     GFGAYICGEETALLESLEGKKGQPRFKPPVPASYGLYGKPTTINNTETFAAVPFIINR
                     GGDAFLALGKP"
     gene            complement(793..1302)
                     /gene="nuoE"
                     /locus_tag="VLN25_06420"
     CDS             complement(793..1302)
                     /gene="nuoE"
                     /locus_tag="VLN25_06420"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HSQ10232.1"
                     /translation="MSAVPKSPDVLSAESCRRIDREIAKYPTGLQQSAVMAALAIAQD
                     QYGWLPEEAINAVADYLAMAPIAVYEVASFYNMYNLRPTGRCKLTLCTNLPCALSGAN
                     QAAAHLKQRLGIGFGETTADGQFTLKEGECLGACGDAPVLLVNNKRMESFMSDERLDA
                     LLDEFRQAK"
     gene            complement(1384..2637)
                     /locus_tag="VLN25_06425"
     CDS             complement(1384..2637)
                     /locus_tag="VLN25_06425"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HSQ10233.1"
                     /translation="MADIKNYTLNFGPQHPAAHGVLRLVLELDGEVIQRADPHIGLLH
                     RATEKLAETRTFLQSVPYMDRLDYVSMMCNEHAYVMAIEKLLGVEVPVRAQYIRVMFD
                     EITRLLNHLLWLGAHGLDVGAMAVFLYAFREREDLMDIYEAVSGARMHAAYYRPGGVY
                     RDLPDAMPQFKANKYKSDQDTRRLNENRQGSLLDFIEDFTRRFPKYVDEYETLLTDNR
                     IWKQRLVGIGVVTPERALQLGVTGPMLRGSGIEWDLRRKQPYEVYDRMEFDIPIGRNG
                     DSYDRYLVRVEEMRQSNRIIRQCIAWLRANPGPVMTDNHKVAPPSRVEMKSNMEELIH
                     HFKLFTEGFHVPAGEVYAAVEHPKGEFGIYLVSDGANKPYRLKIRAPGFAHLQALDEM
                     ARGHMIADAVAIIGTQDIVFGEIDR"
     gene            complement(2640..3236)
                     /locus_tag="VLN25_06430"
     CDS             complement(2640..3236)
                     /locus_tag="VLN25_06430"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HSQ10234.1"
                     /translation="MSRADSLAGALRQVLGDKLQDMIVALGEVTIVVRGDDHLDVSRA
                     LRDDAGLGFEELIDLAGIDYSSYGEDVWDGPRFAVVMHLLSLKHNWRVRVRSICADDE
                     LPIIGSVTDIWNCANWYEREAFDLYGIVFEGHPDLRRILTDYGFVGHPFRKDFPISGH
                     VEMRYDPEQKRVVYQPVSIEPREIVPRVIREDSYGQGR"
     gene            complement(3233..3709)
                     /locus_tag="VLN25_06435"
     CDS             complement(3233..3709)
                     /locus_tag="VLN25_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HSQ10235.1"
                     /translation="MGIEGVLNEGFVTTSVDKLVNWSKTGSLWPMTFGLACCAVEMMH
                     AGAARYDLDRFGIFFRPSPRQSDVMIVAGTLCNKMAPALRKVYDQMAEPRWVISMGSC
                     ANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALIYGIIQLQNKIRRTNTIAR"
     gene            complement(3759..4124)
                     /locus_tag="VLN25_06440"
     CDS             complement(3759..4124)
                     /locus_tag="VLN25_06440"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HSQ10236.1"
                     /translation="MNLDSYLPVLLFILVGLIIGAAPIFMGALLGPHKPDPEKLSPYE
                     CGFEAFEDARMKFDVRYYLVAILFILFDLEIAFLFPWAVVLGDLGAWSVGFWAMMGFL
                     AILTVGFIYEWKRGALDWE"
     gene            complement(4235..4319)
                     /locus_tag="VLN25_06445"
     tRNA            complement(4235..4319)
                     /locus_tag="VLN25_06445"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4283..4285),aa:Leu,seq:gag)
     gene            complement(4426..4863)
                     /gene="secG"
                     /locus_tag="VLN25_06450"
     CDS             complement(4426..4863)
                     /gene="secG"
                     /locus_tag="VLN25_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HSQ10237.1"
                     /translation="MTWLTTLLIVVQVISAIGIIVLVLLQHGKGADMGAAFGGGASGS
                     LFGATGSANFLSRSTAAVATVFFVATLGLAYAGTAVKRPTSGGGGVMDSVPAPSNTAP
                     KDIPVPMAPQGSAPAPAPAVPATPAATATPATPVAPKPADVPK"
     gene            complement(4924..5673)
                     /gene="tpiA"
                     /locus_tag="VLN25_06455"
     CDS             complement(4924..5673)
                     /gene="tpiA"
                     /locus_tag="VLN25_06455"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HSQ10238.1"
                     /translation="MTLRKPLVAANWKMNGSRAANAAWLTAFAPRRASMACEVVICAP
                     AVYLEWLVTAASGIDVGAQDVSEHSPGAFTGDIAATMLVDIGCRWVIVGHSERRQLHA
                     ESDETVAAKSEAALIAGLRPIVCVGESLAEREAGRTLEVVGRQLDAVLARVGAGGLAR
                     GALAYEPVWAIGTGRSASPDQAQDVHAALRAQVASKASAEANGLRILYGGSVKPANAA
                     ELFGQPDIDGGLIGGASLVADDFAAICAAAR"
     gene            complement(5735..6739)
                     /locus_tag="VLN25_06460"
     CDS             complement(5735..6739)
                     /locus_tag="VLN25_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HSQ10239.1"
                     /translation="MRAVAIERPGGPDVLQEVERPRPIPGTGEMLIRVQAAGVNRPDV
                     LQRKGAYAPPPGVSDLPGLEIAGIVEGGDLDGSGWTRGDRVCALVAGGGYAQWCVAPV
                     GQCLPVPRGLSFAEAATLPETYFTVWSNVFDRARLAPGESLLVQGGTSGIGVTAIQLA
                     AALGHAVFGTAGSDDKARACEELGARRGINYRTEDFAAVVKEETAGRGVDVILDMVAG
                     DYVPREVGCLADDGRLVIIALQGGALASLDLAAVLRRRLTITGSTLRPRSVAFKAAIA
                     ERLRAVVWPLIEAGRIKPIVHSTFPLARAADAHRLMESSEHIGKIVLTVGPESADRGQ
                     "
     gene            complement(6751..8775)
                     /locus_tag="VLN25_06465"
     CDS             complement(6751..8775)
                     /locus_tag="VLN25_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HSQ10240.1"
                     /translation="MADTDTAVDPFLWLEEIDGERALDFVRAGNARTLEELESQGSFV
                     PIRDAIREVLDSQDRIPQVEKLGEYLYNFWQDRANPRGLWRRTTWEQFRAPVTSWEIV
                     LDLDTLARDEGENWVWKGAQCLPPAYERCLVTLSRGGGDAAVVREFDLRSRAFVPGGF
                     NVPEAKAAIEWRDLDTVYVATDFGPGSLTDSGYARIVKRWQRGTPLSEAVTMFEGQHS
                     DVLVSPRVDHDPRSFRETFVRLIAFYRSATWLMLGGSMVRLDLPEDAEPALVGRWLVV
                     RLRSPYSTGQGNFPAGALIATEASDFLAGDLNFEALFVPSLRTALQGWTATRNTLVLD
                     ILDNVSSRPVRLTHGPEGWAREDIVLPGLGSAIIAAVDRLQSDEVWITYQDFLTPPTL
                     MLSTPGRPLDTVRSMPAFFDASGMTVEQLEATSTDGTSVPYFVIGLRDRVDGPTLLYG
                     YGGFEISLQPVYNGASGRAWLVRGGTLVIANIRGGGEFGPAWHQAALKANRQRAYDDF
                     MAVANDLIRRGIASPQRLGIMGGSNGGLLVGAVLMQRPDLFGAVVCQVPLLDMRRYNR
                     LLAGASWMAEYGDPDRPEDWDYISRYSPYHNLRDDRCYPPTLFVTSTRDDRVHPGHAR
                     KMYARMSSHGHDAWYYENLEGGHAAAADNEQRARMNAVQYTFLWKALDRR"
     gene            complement(8934..11081)
                     /gene="pnp"
                     /locus_tag="VLN25_06470"
     CDS             complement(8934..11081)
                     /gene="pnp"
                     /locus_tag="VLN25_06470"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HSQ10241.1"
                     /translation="MFEKVTKTFAYGAHTVTLETGEIARQSGGAVVCSMDDTVVLATV
                     VARKEAKPGQDFFPLTVDYIEKTYAAGRIPGGFFKREGRPSEKETLTSRLIDRPIRPL
                     FPDGFFNEVQVIIHVLSIDPEIDPDIPALIGASAALSLSGIPFKGPIGAARVGHIDGE
                     YVINPTATQLKTSKLNLVVAGTERAVLMVESEADQLSEEVMLGAVMHGHAQMQAAINA
                     IHELVRDAGKPAWDWTPAAHNEPLLAKLVHLAGPGLRDAYLTRNKQARTHKINDVYTQ
                     VWAKLGEEATTSGQPKPDDNAVDGILFDLQAKIVRSQILEGEPRIDGRDTRTVRPIEI
                     RLGVLPRTHGSALFTRGETQALVTATLGTKQDEQFVDALQGEYRDRFMLHYNMPPFAT
                     GETGRVGTPKRREIGHGRLAKRSLLAVLPSYDDFQYSLRVVSEITESNGSSSMASVCG
                     ACLAMMDAGVPLKAHVAGVAMGLIKEGNKFAVLTDILGDEDHLGDMDFKVAGSANGIT
                     ALQMDIKIEGITREIMQVALAQAKEGRMHILQKMQDAISVSRGELSAFAPRLIKMKIN
                     PEKIRDVIVKGGAVIRALTEETGTQIDIEDDGTVIIASVDQEKGKEAQRRIQELTAKV
                     EVGKVYDGTVLRLLDFGAIVQLLPGKDGLLHISQIANERVNAVADYLKEGQAVKVKVI
                     EADEKGRVRLSMKAVEGNAPAPQPQAQVQQPQH"
     gene            complement(11278..11547)
                     /gene="rpsO"
                     /locus_tag="VLN25_06475"
     CDS             complement(11278..11547)
                     /gene="rpsO"
                     /locus_tag="VLN25_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HSQ10242.1"
                     /translation="MAVADINKSEIVAKYQRAQADTGSPEVQVALLTARINELTDHFK
                     AHVKDHHSRRGLLKMVSRRRKLLDYLKGKNPEAYKGLIDSLGLRK"
BASE COUNT         2043 a         3911 c         3565 g         2083 t
ORIGIN      
        1 ggcttgccga gcgccaggaa ggcatcaccg ccgcgattga tgatgaaggg caccgccgca
       61 aaagtctcgg tgttgttgat cgtcgtcggc ttgccgtaca gaccgtagct ggccgggacc
      121 ggcggcttga agcgtggctg gcccttcttg ccttcgagcg actcgagcag cgcggtttcc
      181 tcgccgcaga tgtaggcacc gaagccgtgc gaggcatgca ggtcgaaact gaacgacgag
      241 ccgaggatgc ctctgccgag gtagccggcc gcgcgcgcct cgtcgagcga ttcctcgaag
      301 cgctcgtagt cggtccagat ttcgccgtgg atgtagttgt agccgacgga aactcccatt
      361 gcgtaggccg caatcgccat gccctcgatg acgatgtgcg ggttgtagcg caggatgtct
      421 cgatccttga acgtgccggg ctcgccttcg tcggagttgc aaacgacgta cttctgtccc
      481 ggaaactgtc gcggcatgaa gctccacttg agccccgtcg ggaagcccgc gccgccgcgg
      541 ccacgcagtg ccgacttctt cagttccgcg atgaccgcgt caggcgccat gcgctccgtc
      601 aggatcttgc gcagggccgc gtacccgccc cgctgcatgt agtccttcag gcgccagttg
      661 gtcccgtcca gtccggcgag gatcatgggt tcgatgtgac ggccgtggaa gcaggtctgc
      721 gcactcacag ttgagcctcc gcgctgcgcg ctgcggcgcg agcttgcttg gggcggcccg
      781 gcgctgcgct catcacttcg cctgcctgaa ctcgtcgagc agcgcatcga gccgttcgtc
      841 gctcatgaag ctttccatgc gcttgttgtt caccaacagc accggtgcat cgccgcacgc
      901 gcccaggcac tccccttcct tgagggtgaa ctggccatcc gccgtggtct ctccaaatcc
      961 gatgcccagg cgctgtttca ggtgtgcagc cgcctgattc gctcccgaca gcgcgcacgg
     1021 caaattcgtg cagagcgtca acttgcagcg gccggtcggc cgcaggttgt acatgttgta
     1081 gaacgaggcg acctcgtaaa cggcgatcgg tgccatggcc agatagtcgg ccaccgcgtt
     1141 gatggcctcc tctggcagcc agccgtactg gtcctgtgca atcgcgagcg ctgccatcac
     1201 ggccgactgc tggagtccgg tcgggtattt ggcgatctcg cgatcgatcc gacggcacga
     1261 ctcggccgac aggacatctg gagacttggg tacggcgctc attgctttac gtttgcccct
     1321 tacgacgctg cgcgccgatc cccccgaggg ggagcgagct ccttgggagc ggcccggcgg
     1381 cgctcatcgg tcgatctccc cgaacacgat gtcctgcgtg ccgatgatcg cgaccgcgtc
     1441 ggcgatcatg tgaccacgcg ccatctcgtc gagcgcctgc aggtgcgcga agcccggcgc
     1501 cctgatcttc aggcgatacg gcttgtttgc gccgtccgat acgagataga tcccgaactc
     1561 tcccttcgga tgctcgacgg cggcgtacac ctctcccgcc ggaacatgga agccctcggt
     1621 gaagagcttg aagtgatgga tcagctcttc catgttcgac ttcatctcga cgcgcgatgg
     1681 cggcgcgacc ttgtggttgt cggtcatcac cgggcctgga ttcgcacgca gccaggcgat
     1741 gcactgccgg atgatgcggt tgctctgtcg catttcctcg acacgcacga ggtaccggtc
     1801 gtagctgtcg ccgttgcgtc caatcgggat gtcgaactcc atccggtcat agacttcata
     1861 gggctgcttg cgccgtaggt cccattcgat acccgagccg cgcagcatcg gcccggtgac
     1921 tcccagttgc agcgcgcgct cgggtgtcac gacaccgatg cccaccagtc gctgcttcca
     1981 gatccggttg tcggtcagca acgtctcgta ctcgtcgaca tacttcggga agcggcgggt
     2041 gaagtcctcg atgaagtcga gcagcgagcc ctgacgattc tcgttcaggc ggcgggtgtc
     2101 ctggtcgctc ttgtacttgt tcgccttgaa ctgcggcatc gcatccggca ggtcgcggta
     2161 gacgccgcct ggccggtaat aggccgcgtg catgcgcgcg cccgacaccg cctcgtagat
     2221 gtccatcagg tcttcgcgct cgcggaacgc atacaggaat accgccatcg cgcccacatc
     2281 gagaccgtgt gcaccgagcc aaagcaggtg attcagcagc cgcgtgatct cgtcgaacat
     2341 gacgcggatg tactgcgccc gcaccggcac ctcgacgccg aggagcttct cgatcgccat
     2401 cacgtaggcg tgctcgttgc acatcatcga gacgtagtcg agtcggtcca tgtacggcac
     2461 cgactgaagg aacgtgcggg tctcggcgag cttctcggta gcccggtgca gcagccctat
     2521 atgcggatcg gcgcgctgga tcacctcccc atcgagttcc agcaccagcc gcagcacccc
     2581 atgcgcagca ggatgctgag ggccaaagtt caacgtatag ttcttgatat cagccacgat
     2641 cagcgtccct gcccgtagct gtcttcgcgg atcaccctcg ggacgatctc gcgcggctcg
     2701 atcgacaccg gctggtagac cacccgcttc tgttccgggt cgtagcgcat ctcgacgtga
     2761 cccgagatcg gaaagtcctt gcggaacgga tgtccgacga atccgtagtc ggtcaggatc
     2821 cggcgcagat ccggatggcc ctcgaacacg atgccgtaca agtcgaaggc ctcgcgttcg
     2881 taccagttgg cgcagttcca gatatccgtt acggagccga ttattggaag ctcgtcgtcg
     2941 gcgcagatgc tgcgcacccg cacacgccag ttgtgcttca gcgacagcag gtgcatcacc
     3001 accgcgaacc gcggtccgtc ccatacgtcc tcgccatagg acgaatagtc aattcccgcg
     3061 agatcgatga gttcctcgaa tcccagacct gcatcgtcgc gcagcgccct cgacacgtcg
     3121 aggtggtcgt caccgcgcac gacgatcgtc acctcgccga gcgcgacgat catgtcctgc
     3181 agcttgtcgc cgagcacctg tctgagcgca ccggccagtg aatcggcgcg gctcatcggg
     3241 cgatcgtgtt ggtgcggcga atcttgttct gcaactggat gattccgtag atcagcgcct
     3301 cggccgtcgg cgggcagcct ggcacgtaga catcgaccgg cacgatgcgg tcgcagccgc
     3361 gaaccacgga gtagctgtag tggtagtagc cgccaccgtt tgcgcacgaa cccatgctga
     3421 tgacccagcg cggctcggcc atctggtcgt agaccttgcg cagcgcgggc gccatcttgt
     3481 tgcacagcgt cccggccacg atcatcacgt cggactgtcg aggactcggc cggaagaaaa
     3541 tgccgaagcg gtcgaggtcg tagcgcgcgg cccccgcgtg catcatctca acagcacagc
     3601 aggccaagcc gaacgtcatc ggccatagcg aacccgtctt ggaccagttc accagcttgt
     3661 caacgctggt cgtgacgaat ccctcgttga ggacgccctc gatacccatc agcgctcccc
     3721 tctgaagcgc agcctgtgca agtggagcgg tggcatcgtt actcccagtc gagcgcgccg
     3781 cgcttccatt cgtagatgaa ccccacagtc aggatggcga ggaatcccat catggcccag
     3841 aagccgacgc tccacgcccc gaggtcgccg agcacgacgg cccaaggaaa gaggaaggca
     3901 atctcgagat cgaacaggat gaacaggatc gccacgaggt agtagcgcac gtcgaacttc
     3961 atgcgcgcgt cctcgaacgc ctcgaagccg cactcgtagg gcgagagttt ctcgggatcc
     4021 ggcttgtgcg gcccgagcag cgcgcccata aaaatcggcg ctgccccgat gatcagtccg
     4081 acgaggatga acaacaggac gggaaggtag ctgtcgagat tcacgcgggt cagccttccg
     4141 gctcacattc ctatgaaacc gccttgcggc ggactcaaaa cgacgcccgg gttgcacttt
     4201 cacactgacg tcgccgcagc gacaaagctc aaattggtgc cgacggtgag actcgaactc
     4261 acacggcttt cgccgccacc ccctcaagat ggtgtgtcta ccaattccac cacgtcggca
     4321 aggtgcaaaa cccgcgggat tctacttgaa tcactcacgc ttcgcggccg ttgtgagagc
     4381 gagaaacgct gaacagcgca ctcgccgcgg catgcagcgc gggttttact tgggaacgtc
     4441 ggcgggcttt ggcgcgaccg gagtcgccgg cgtggccgtc gctgccggtg ttgccggcac
     4501 cgcgggcgcc ggtgccggag ccgacccctg cggggccatg ggcaccggaa tatccttcgg
     4561 tgcggtgttc gacggtgcgg gcaccgagtc catgacgccg ccaccgccgc tcgtcggtcg
     4621 cttcacggcc gtgccggcat acgccagtcc gagcgtggcg acgaaaaata cagtggcgac
     4681 cgcggccgtg ctgcgtgaca ggaagtttgc cgagccggtc gctccgaaga ggctgccgga
     4741 agcgccgcca ccgaacgcgg cgcccatgtc ggcgcccttg ccgtgctgta gaagcaccag
     4801 cacgatgatc ccgatcgcag aaatcacctg caccacgatc agcaaagtgg tcaaccaagt
     4861 catctcaagt cccttctgtc caattccgtt gcaatctatt tcgtaggcgc gtcggcagca
     4921 gtgtcatcta gcggccgcgc agatcgccgc gaagtcatcc gcgacgagcg aggcgccgcc
     4981 gatcaatccc ccgtcgatat ccggctggcc aaagagttcc gcagcattgg caggtttcac
     5041 acttccaccg taaagaatcc tcaagccgtt cgcttctgca gaggccttgg acgcaacctg
     5101 tgcgcgcagc gccgcatgca cgtcctgggc ctgatccggc gacgcgctgc gccccgttcc
     5161 gatggcccac accggctcgt acgccagagc accccgcgcc agcccccccg cccccacgcg
     5221 cgccagcacc gcgtcgagtt gccgaccgac gacttcaagc gtccggcccg cttcgcgttc
     5281 cgccagcgac tcgccgacac agacgatggg ccgcagcccc gcgatcagag ccgcctccga
     5341 ctttgccgcc acggtctcgt cggattcggc atgcagctgg cgccgttccg aatgcccgac
     5401 gatgacccac cggcatccga tgtcgacgag catcgtcgca gcgatgtcgc ccgtgaatgc
     5461 cccgggtgaa tgctccgaga catcctgagc gccgacatcg ataccgctcg cagccgtcac
     5521 cagccactcc agatagaccg ccggtgcgca gatcacgacc tcgcacgcca tcgacgcacg
     5581 acgcggcgcg aacgccgtca gccatgccgc attggcggcc cgcgaaccgt tcatcttcca
     5641 gttcgccgca acgagaggtt ttcgtagagt catgatcgga aaaagcccgc cattgtaatt
     5701 ggccgggccg agccgaccaa acgcatgcac gcggtcattg gcctcggtcc gccgactccg
     5761 gcccgaccgt caggacgatc ttgccgatgt gttcgctgga ctccatcagc cgatgcgcat
     5821 ccgcggcccg cgcaagcgga aacgtcgaat gcactatcgg cttgatgcgt cccgcttcga
     5881 tcagtggcca cacgacagcg cgcagccgct cggcaatcgc tgccttgaac gcaaccgaac
     5941 gcgggcgcag tgtcgacccg gtgatggtca accgccgccg cagcactgcc gccagatcga
     6001 gcgacgccag cgcgccacct tgcagggcaa tgatcaccaa gcgtccatcg tctgcgaggc
     6061 agcctacttc gcgcggcaca tagtcgccgg ccaccatgtc gaggatgacg tccacgccgc
     6121 gtcctgcggt ctcttctttc acgaccgcgg caaagtcttc ggtgcggtag ttgatgcccc
     6181 gccgcgctcc gagttcttcg caggcccgcg ccttgtcgtc cgatcccgcg gttccaaaca
     6241 ccgcatgacc gagggctgcc gccagctgga tcgccgtcac gccgatgccg ctggtgccgc
     6301 cctggaccag caaggattcg cccggtgcca gccgggcccg atcgaacaca ttgctccaga
     6361 ccgtgaagta cgtctcaggc agcgtcgccg cctcggcaaa tgacaagccg cgcggaaccg
     6421 gaaggcattg tccgacggga gcgacgcacc actgcgcata tcccccaccc gccaccagcg
     6481 cgcacacgcg atctccacgc gtccatccac tgccgtcgag gtcgccgcct tccacaattc
     6541 cggcaatttc cagtccgggc agatccgaga ctcccggtgg cggcgcatac gcccccttgc
     6601 gctgcagaac atcggggcgg ttcactcccg cggcctgtac acggatcaac atttcgcccg
     6661 tgcccgggat cgggcgcggg cgctcgacct cctgcaggac gtccggtccg ccaggccgtt
     6721 cgatggcgac agcgcgcatc gtgacctcac tcatcgtctg tccagcgcct tccacagaaa
     6781 cgtgtattgc acggcgttca tcctggcccg ctgctcgttg tctgccgcgg ccgcatggcc
     6841 gccctcgagg ttctcgtagt accaggcgtc atggccgtga ctgctcatcc gcgcgtacat
     6901 cttgcgcgca tggcccggat gaacccgatc atcgcgggtc gaagtgacga acagcgtggg
     6961 cggataacag cggtcgtcgc gcaggttgtg atacggcgag tacctggaaa tgtagtccca
     7021 atcttcggga cgatcgggat caccgtactc ggccatccag ctcgcgcccg ccagcagcct
     7081 gttatagcgc cgcatgtcca gcagcggcac ctggcacacg acagccccga acaggtccgg
     7141 ccgctgcatc agcaccgccc ccacgagcag tccgccgttg ctgccaccca tgatgccgag
     7201 acgctgcggc gacgcgatgc cgcgtcggat caggtcgttc gcgacggcca tgaagtcgtc
     7261 gtaggcacgc tggcggttcg ccttgagcgc ggcctggtgc caagcgggac cgaactcgcc
     7321 accgccacga atgttggcga tgacgagcgt gccgccgcgc accagccagg cccggccgct
     7381 ggccccgttg tagaccggct gcagcgagat ctcgaagccg ccgtagccat aaagcagtgt
     7441 cgggccatca acgcgatcgc gaagcccgat cacgaagtac ggcacgctgg ttccgtcggt
     7501 cgacgtcgct tcaagctgct cgaccgtcat gcccgaggca tcgaaaaaag ccggcatgga
     7561 cctgacagtg tcgagcggcc ggccgggcgt cgacaacatc agcgtcggcg gcgtcaggaa
     7621 gtcctgatag gtaatccaga cttcgtcgga ctgcaggcga tcgaccgccg cgatgatcgc
     7681 tgaacccagc cccggcagga cgatgtcctc gcgcgcccag ccctccgggc catgcgtcag
     7741 gcgcacgggt cggctgctga cgttgtcgag gatgtcgagg acgagcgtgt tgcgcgttgc
     7801 cgtccagccc tgcaacgcag tccgaaggga agggacgaac agtgcttcga aattgagatc
     7861 cccggcgaga aaatcggaag cctccgtcgc gatcagcgcg cccgctggaa agttgccctg
     7921 cccggtcgag tagggactgc gcaggcgaac caccagccag cggcccacca gcgcaggctc
     7981 ggcgtcctcg ggcaagtcga gcctgaccat tgagccgcca agcatgagcc aggttgcgct
     8041 ccggtagaac gcgatcaggc ggacgaacgt ttcgcggaac gatcgcgggt cgtgatcgac
     8101 cctcgggctg accagaacgt cactgtgctg gccttcgaac atggtgaccg cctccgacag
     8161 cggcgtccca cgctgccagc gcttgacgat gcgcgcatag ccgctgtccg tcagcgagcc
     8221 ggggccgaag tcggttgcca cgtacacggt gtcgagatcg cgccactcga tcgccgcctt
     8281 cgcctcggga acgttgaatc ccccaggcac gaacgcgcgg gagcgcagat caaattcgcg
     8341 cacgacggcc gcatccccgc caccacggga cagcgtgacg aggcagcgct cgtacgccgg
     8401 cggcaggcac tgggcgccct tccagaccca gttctcaccc tcgtcgcgcg cgagggtgtc
     8461 cagatcgagc acgatctccc acgacgtcac cggcgcccgg aactgttccc acgtagtccg
     8521 acgccagagg ccgcgcggat tcgcccggtc ctgccagaag ttgtagaggt actcacccag
     8581 cttctcgacc tgcgggattc gatcctgcga gtcgaggacc tcgcgaatcg catcacggat
     8641 cggcacgaag ctcccctggc tttcaagctc ttcgagcgtg cgggcgttgc cggcgcgtac
     8701 gaagtcgagc gcgcgctcgc cgtcgatctc ttccagccac aggaagggat ccaccgcggt
     8761 gtcggtatca gccaatgtga ttgcactcca agtaaggcag aatcttgtgc cccgtttgcg
     8821 aatcaatgca cgctgcaagc cggccgcagc gaccggcagc gttgcagctc ggttctcgaa
     8881 aggcagcgaa aagcaaaagg ggcgccgagg cgccccttcc cgcggacgtc cgcctagtgc
     8941 tgcggctgtt gaacctgcgc ctgcggttgc ggggccgggg cgttgccctc gaccgccttc
     9001 atggacagtc gcacgcgacc cttctcgtcg gcctcgatga ccttgacctt cacggcctgt
     9061 ccttccttca gatagtctgc gaccgcattg acgcgctcgt tggcaatctg ggaaatgtgc
     9121 agcaggccat ccttgcccgg cagcagctgc acgattgcgc cgaagtccag caggcgcagc
     9181 acggtgccgt cgtagacctt gccgacctcg accttcgcgg tcaattcctg aatacggcgc
     9241 tgggcttcct tgcccttttc ctggtcgacg ctcgcgatga tgacggtgcc gtcatcctcg
     9301 atgtcgatct gcgtgccggt ctcctcggtc agcgcacgga tcaccgcacc gcccttgacg
     9361 atcacatcgc ggatcttctc gggattgatc ttcatcttga tcaggcgcgg cgcaaacgcg
     9421 gacagctcgc cgcgcgacac ggaaatcgca tcctgcatct tctgcaggat gtgcatgcgg
     9481 ccttccttcg cctgcgcgag cgccacctgc atgatctcgc gggtgatgcc ctcgatcttg
     9541 atgtccatct gcagcgcggt gatgccgttg gcagacccgg ccaccttgaa gtccatgtcg
     9601 ccgaggtggt cctcgtcgcc gaggatatcg gtcagcaccg cgaacttgtt gccttccttg
     9661 atcaggccca tcgcaacgcc ggcgacgtgc gccttgagcg gcacgccggc atccatcatc
     9721 gccaggcagg caccgcaaac agaggccatc gacgacgagc cattcgactc ggtgatctcg
     9781 gaaacaacgc gcagcgagta ctggaagtcg tcatagctcg gcagcacggc cagcaaagaa
     9841 cgcttggcga gccgaccgtg tccgatttcg cggcgcttcg gggtaccgac gcgcccggtc
     9901 tctccggtgg cgaagggcgg catgttgtag tggagcatga agcgatcgcg gtactcgccc
     9961 tgcagcgcgt cgacgaactg ctcgtcctgc ttggtgccga gggtggccgt gaccagcgcc
    10021 tgggtctcac cgcgggtgaa aagcgccgag ccgtgcgtgc gcggcagcac accgagacgg
    10081 atctcgatcg ggcgcaccgt acgcgtgtcg cgaccatcga tgcgcggctc gccctcgagg
    10141 atctggctgc gcacgatctt ggcctgcagg tcgaacagga tgccgtcgac cgcgttgtcg
    10201 tccggctttg gttgtccgct ggtcgtcgct tcttccccga gcttggccca cacctgcgtg
    10261 tagacgtcat tgatcttgtg cgtgcgcgcc tgcttgttgc gggtcaggta ggcatcacgc
    10321 aggccaggac cggccagatg aaccaacttt gcgagcagcg gctcgttgtg ggccgccggt
    10381 gtccagtccc aggccggctt gccggcgtcg cgcaccagct cgtgaatcgc attgatcgcg
    10441 gcctgcatct gggcgtggcc gtgcatcacg gcgccgagca tcacttcttc cgagagctgg
    10501 tcggcttcgg actcgaccat cagcacggcg cgctccgtgc cggcgaccac gaggttcagc
    10561 ttcgaggtct tgagctgggt cgcggtcgga ttgatcacgt actcaccatc gatgtggccg
    10621 acgcgggccg caccgatcgg tcccttgaac ggaatgcccg acagcgacag cgccgcagag
    10681 gcaccgatca gcgccgggat gtcagggtcg atctccggat cgatcgacag cacgtggatg
    10741 atgacctgga cttcgttgaa gaagccatca ggaaacagcg gacggatagg ccggtcgatc
    10801 aggcgcgagg tcagcgtttc cttttccgag ggacggcctt cgcgcttgaa gaaaccgccc
    10861 gggatgcggc cggcggcgta ggtcttttca atgtagtcca cggtcagcgg gaagaagtcc
    10921 tggccgggct tggcttcctt gcgtgccacc acggtggcca gcacgacggt gtcatccatc
    10981 gagcacacga cagcgccgcc cgattgccgc gcaatctcgc ccgtctccag cgtgacggta
    11041 tgcgcgccgt atgcaaacgt cttggttact ttttcgaaca cttggaattc ctttcttgtt
    11101 gagcgcggtc gcacgagatc caaccctcgg cgacccggca cgctgcggta tcgacgactc
    11161 ggcgcggggt ttccgccctt acgtccatat cgcagcgcgc attccggccg gacaacgaca
    11221 accggcccgc gcagatcact ccacgcaggc tggctgcact tgcatccggg tcattgacta
    11281 cttgcgcagt ccgaggctgt cgatcagccc cttgtacgcc tcggggttct tgcccttgag
    11341 gtagtcgaga agcttgcggc gccgactgac catcttgagc agaccccgac gtgagtggtg
    11401 atccttcacg tgggccttga agtggtcggt cagctcgttg attcgggccg tcagaagcgc
    11461 aacctgcact tcaggcgatc cggtgtcggc ctgcgcgcgc tgatacttcg cgacgatttc
    11521 cgacttgttg atatctgcaa ctgccatggt ttccctttca gatcaatcag ctagacacgc
    11581 ggacacggaa ggatggccgt gc
//
LOCUS       DATIRT010000284         6761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_141260, whole genome shotgun sequence.
ACCESSION   DATIRT010000284 DATIRT010000000
VERSION     DATIRT010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6761
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141260"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..1229)
                     /locus_tag="VLN25_06480"
     CDS             complement(132..1229)
                     /locus_tag="VLN25_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HSQ10243.1"
                     /translation="MSTEPAPAEALRVEGVHKAFGDFVALRSIDLVVPPGELVCFLGP
                     SGCGKTTLLRIIAGLEQQSRGRILQNGRDVSFAPPAARDYGIVFQSYALFPNLSIAQN
                     VSYGLVNRRKGREEIASRVRELLALVGLSDAGSKYPSQMSGGQQQRVALARALATSPS
                     LLLLDEPLSALDAKVRERLRGEIRQLQRRLRVTTIMVTHDQEEALSMADRVVVMNQGN
                     IEQVGTPSDVYERPATPFVADFLGKANVLKGVALGAGRYRVGSAELALPADEHPVGRS
                     VRIYVRPEDRHVEGDIASLPNRLRGRIARIDYLGTFCLAELRCEALGQPMLISYSLNQ
                     LHDLAVREGGEVEIALRVDRVRVFADAPTAA"
     gene            complement(1317..2345)
                     /locus_tag="VLN25_06485"
     CDS             complement(1317..2345)
                     /locus_tag="VLN25_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_function: GO:0033226 - 2-aminoethylphosphonate binding
                     [Evidence IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HSQ10244.1"
                     /translation="MKLLTIAGKAVAAAALAAAAFGVNAQKTQLTVYTALETDQLKAY
                     QAAFEKANPTIEIVWVRDSTGIITAKLLAEKANPKADVIMGVAATSMAIFDKDGMLTP
                     YAPAGLNRISAQYRDPKNPPAWVGMDVWGATICFNTAEAAKRNIPKPETWKDLTKPVY
                     KGQIVMPHPASSGTGFFDVSAWLQLWGDKEGWAFMDGLHENIAQYMHSGSRPCAAAAA
                     GEYTVGISFEYRANREKARGAPIELVFPKEGLGWDLEASGIVKGTKNLDAAKKLMDWS
                     VSDEAMGLYANNFAIVAVPALNKPLPNVPANYASLLVKNDFAWAAANRDKILAEWSKR
                     YESKAAPK"
     gene            2565..3293
                     /locus_tag="VLN25_06490"
     CDS             2565..3293
                     /locus_tag="VLN25_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate utilization associated
                     transcriptional regulator"
                     /protein_id="HSQ10245.1"
                     /translation="MAPPSTSNVVPHPTIALLRANSLASAAQEEIERLILDGSIAPGA
                     KLTEAWLSERLGVSRGPIREAFRMLEEAGLVRQEKNRGVFVRDIPHDEAAEIYDLRAV
                     MDELVGRRLAESVTAVQMKALRAIVERMEQAVRAEDRDAYHLLNLQFHDRLVEFAGNR
                     KLATIYNKLVKELALYRRRNLVDLTVLPQSAAEHRAIVKAIASGDADQAGHAMYDHVM
                     ESKQRALRSAGLAAPARARAGRRG"
     gene            3290..4510
                     /gene="phnA"
                     /locus_tag="VLN25_06495"
     CDS             3290..4510
                     /gene="phnA"
                     /locus_tag="VLN25_06495"
                     /EC_number="3.11.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047400 - phosphonoacetate hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019636 - phosphonoacetate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetate hydrolase"
                     /protein_id="HSQ10246.1"
                     /translation="MSSMFEVNGRQYSMPKEPTVIICVDGCEPDYIAQAVAGGQMPFM
                     KRVLAEGTALIGDCVVPSFTNPNNLSIVTGAPPSVHGICGNFLFDTATGAEVMMNDPK
                     WLRAPSILAGLTDRGASVTVITAKDKLRKLLGHGMKGICFSSEKANQVSEQENGIDAV
                     LPLVGMAVPDVYIAELSEFVFAAGVRLLETRRPDVMYLSTTDYVQHKHAPGDPDANRF
                     YAMLDRYLARMDALGAVVGVTADHGMNAKTKLDASPNVIFLQDLLDEWLGSERARVIL
                     PITDPYVVHHGALGSFATVYLPDDVIHDVASRLRDLIGIEVVLTRAEAAARFELPPDR
                     IGDLVVVSERSVVLGTGKARHDLSQLELPLRSHGGISEQRVPIILNRRIAGLDSNRRW
                     RNFDVFDLALNHAR"
     gene            4513..5952
                     /gene="phnY"
                     /locus_tag="VLN25_06500"
     CDS             4513..5952
                     /gene="phnY"
                     /locus_tag="VLN25_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde dehydrogenase"
                     /protein_id="HSQ10247.1"
                     /translation="MNAPTLPLRHESLRIAGESVATERNFEVRYPYTGEVVATVPKAT
                     VDDVRLAIASARAYRARLTRYERYRILMRAGELIAARRDEIARTITLESGLCLKDTRY
                     EVGRASDVLLFAANQALVDDGQVFSCDLTPHGRSRKVYTLREPLQGIITAITPFNHPL
                     NQVIHKVAPAVATNNRVVLKPSEKTPLAAYMLADILYEAGLPPPMLSVVTGDPREIAD
                     ELLTHPDVDLVTFTGGVAVGKAIAAKAIYKRQVLELGGNDPIIVMDDADVEEAATLAA
                     SGSYKNSGQRCTAVKRMLVHASVADDFVVRLVAKSKAVKCGDPLDPATDMGTVIDEPA
                     AVEFERRVNDAMDAGARLLLGNERRGALYAPTVLDHVQPSMRLVKEETFGPVSPVIRF
                     TSLDEAIAVANSTAYGLSSSVCTNRLDVITRCIAELNVGTVNVREVPGYRLELTPFGG
                     IKDSGLGYKEGVQEAMKSFTNVKTYSLPW"
     gene            6007..>6761
                     /locus_tag="VLN25_06505"
     CDS             6007..>6761
                     /locus_tag="VLN25_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HSQ10248.1"
                     /translation="MTTLLNLLAAIALLVWGTQIVRTGVLRVYGAALRQVLRRSMNGR
                     AAAFFAGVGVTGLLQSSTATSLLTSSFVSQGLLTTSAALAVMLGADVGTAAMVQLFSL
                     DLSWLAPLAIVVGVVVYLSRRNERAGQIGRIVLGLGVMILALQLVTAATAPLAHGAGV
                     KTFLAQISGDLLLDVLIGAVFAVMCYSSLAVVLLVAMLAGSNVLPAHVALPLVLGANL
                     GSGILALLTTWRSDAVMRRVPLGNFVFKAVGVTL"
BASE COUNT         1164 a         2235 c         2170 g         1192 t
ORIGIN      
        1 agaaaaacga agagcgcgac tgccagcagc accaggccgc cgcgcgcagc cagcgcctcg
       61 cccgcgagcg ctgcgcgcga cggcgcgcgg ctcacgttca tgtcggcagg actggcactc
      121 accgggcgtt ctcaggcggc agtgggggca tccgcgaaga cacgcacacg atcgacgcgc
      181 agcgcgatct cgacttcgcc gccctcacgc acggcgagat catgcagctg gttgagcgag
      241 taggagatca gcatcggctg accgagcgct tcgcagcgca gttcagccag acagaacgtg
      301 ccgagatagt cgatgcgcgc gatccggccc cgcaggcggt tcggcagcga tgcaatgtcg
      361 ccctcgacat gccggtcctc gggccggacg tagatccgca cactgcggcc gaccggatgc
      421 tcatcggccg gcagcgccag ttcggccgat cccacgcgat agcgtcccgc gccgagcgcg
      481 actcccttga ggacattggc cttgccgagg aagtcggcca caaacggcgt cgccggacgt
      541 tcgtagacat cgctgggggt ccccacctgc tcgatgttgc cctggttcat cacgacgacg
      601 cgatcggcca tcgacagcgc ctcttcctgg tcgtgcgtga ccatgatggt cgtcacccga
      661 agccgccgct gcagttgccg gatctcaccg cgcaggcgct cgcgtacctt ggcatcgagc
      721 gctgacaggg gttcatccag cagcagcagg ctcggcgacg tggcgagtgc ccgcgcaagc
      781 gcgacgcgct gctgctggcc accggacatc tggctggggt acttgctgcc cgcatccgaa
      841 agcccgacca gggccagcag ttcccgcaca cgcgacgcga tttcctcccg ccccttgcgc
      901 cggttgacca gtccatagga aacgttctgc gcaatcgaga gattggggaa caacgcgtac
      961 gactggaaca cgatgccgta gtcgcgcgcc gccggcggtg cgaaggaaac atcgcgtccg
     1021 ttctgcagga tgcggccgcg gctctgctgc tcgagcccgg cgatgatccg cagcagcgtg
     1081 gtcttgccgc agcccgaggg cccgaggaag cacaccagct cacccggcgg aacgacgagg
     1141 tcgatcgacc tgagcgcaac aaagtccccg aaggccttgt gcacgccctc gacccgcagg
     1201 gcttcagccg gcgccggttc ggtactcatt cagtttcgca aaaaagagcg ccgggtgaac
     1261 ggccacttcg ttcaacccgg cgatctgttc gggcggcaga gccggcccga atgacgtcac
     1321 ttcggtgcgg ccttcgactc gtagcgcttg ctccactcgg ccagaatctt gtcgcgattg
     1381 gcggcagccc aggcgaagtc atttttcacc aggagactcg catagttggc agggacgttc
     1441 ggcaagggct tgttgagcgc cggcacggcg acaatggcga aattgttcgc gtacaggccc
     1501 atggcctcgt ccgagaccga ccaatccatc agtttcttcg ccgcgtcgag gttcttcgtg
     1561 cccttgacga tgccactggc ttccaggtcc cagcccaggc cttccttcgg gaagacaagc
     1621 tcgatcggcg caccacgcgc cttttcgcgg ttggcgcggt actcgaacga aattccgacg
     1681 gtgtactcac ctgctgcggc ggcagcgcac ggacgcgatc ccgagtgcat gtactgcgcg
     1741 atgttttcgt gcaggccgtc catgaaggcc cagccttcct tgtcgcccca caactgcagc
     1801 caggcgctga catcgaagaa gccggtgccc gaagacgccg gatgcggcat cacgatctgg
     1861 cccttgtaga ccggcttggt caggtccttc caggtctcgg gcttcgggat gttgcgcttg
     1921 gccgcctcgg ccgtgttgaa gcagatcgtc gcgccccaca cgtccatgcc gacccaggcc
     1981 ggaggattct tcggatcgcg gtactgcgcc gagatccgat tcaggcccgc gggcgcatac
     2041 ggcgtcagca tgccgtcctt gtcgaagatc gccatgctgg tcgcggccac acccatgatc
     2101 acgtcggcct tgggattggc cttttcggcc aggagcttcg cggtgatgat gccggtcgaa
     2161 tcgcgcaccc agacgatctc gatcgtcggg ttggccttct cgaaggcggc ctgataggcc
     2221 ttgagttggt cggtttcgag cgcggtgtag accgttagct gggtcttctg cgcattgaca
     2281 ccgaacgcag cggccgccag ggcagcggcg gcgaccgcct tgccggcgat ggtaagcagt
     2341 ttcatcgact cctccttaga gccccgtacg tgaaatcttg gcggttcgtc acaggacgga
     2401 aacgaacagc cggcgagcct agcgttcctg cttgacgctt cggtgacggt tctgcgcccg
     2461 cctgcgaagc aaccccgatc gagtcgaggg cagtctacgc agccggaaaa ttcggtgtca
     2521 attgtttttt gcagattgtt tacaattcgg tgtacgctga cgccatggct ccaccgtcca
     2581 cctccaatgt cgttccgcat ccgaccattg cgctgttgcg cgccaattcg ctcgctagcg
     2641 ccgcacagga ggagatcgag cgcctgatcc tggacggttc gatcgcgccg ggtgccaagc
     2701 tcaccgaggc ctggctgtcg gaacgcctcg gcgtctcgcg cggaccgatc cgcgaggcct
     2761 tccgcatgct cgaggaagcc ggactcgtcc gccaggagaa gaatcgcggc gtgttcgtgc
     2821 gcgacattcc gcacgacgaa gccgccgaga tctacgacct gcgtgccgtg atggatgaac
     2881 tggtcggccg gcggctcgcc gagtcggtga ccgcggtgca gatgaaggcg ctgcgcgcca
     2941 tcgtcgagcg catggaacag gcggtccgcg ccgaggaccg cgatgcgtac cacctgctga
     3001 acctgcagtt tcacgaccgc ctggtcgagt ttgcgggtaa ccggaaactg gcgacgatct
     3061 acaacaagct ggtcaaggaa ctggcgcttt accggcggcg caacctggtg gatctcacgg
     3121 tcctgccgca atcagccgcc gagcatcgcg ccatcgtcaa ggccatcgcc tccggggacg
     3181 ccgatcaggc gggccatgcg atgtatgacc acgtcatgga aagcaagcag cgcgccctcc
     3241 gcagcgccgg actcgccgcg ccggcccgcg cgcgggcggg acgacgagga tgagcagcat
     3301 gttcgaggtc aacggccggc agtactcgat gccgaaagaa cccaccgtca tcatctgcgt
     3361 cgacggctgc gaaccggact acatcgcgca ggcggtcgcc ggcggccaga tgccgttcat
     3421 gaagcgcgtg ctggccgaag gcaccgcgct gatcggcgac tgcgtcgtgc cgagcttcac
     3481 caatcccaat aacctgtcga tcgtgaccgg cgctccgccg tcggtgcacg gcatctgcgg
     3541 caacttcctg ttcgataccg cgaccggcgc cgaagtcatg atgaacgatc cgaagtggct
     3601 gcgcgcgccc agcatcctcg ccggactgac ggaccgtggc gccagcgtga ccgtcatcac
     3661 ggccaaggac aagctcagaa aactgctcgg ccacggaatg aaggggatct gcttctcttc
     3721 cgagaaggca aaccaggtca gcgagcagga aaacggcatc gacgccgtgc tgccgctggt
     3781 cggcatggca gtccctgacg tctacatcgc cgagttgtcc gagttcgtct tcgccgccgg
     3841 cgtgcgactg ctcgagaccc gccggcccga cgtgatgtac ctctcgacca ccgactacgt
     3901 gcagcacaag cacgcgcctg gcgatcctga cgccaaccgg ttctacgcca tgctcgaccg
     3961 atacctggcc cggatggacg cgctcggtgc ggttgtcggc gtgaccgcgg accacggcat
     4021 gaacgcgaag acgaagctcg acgcgagccc gaacgtgatc ttcctgcagg acctgctcga
     4081 cgagtggctg ggcagtgaac gtgcccgcgt gattttgccg atcaccgatc cgtacgtcgt
     4141 gcaccacggg gcactgggtt cgtttgcaac ggtctacctt cccgacgatg tgatccacga
     4201 cgtcgcgtcg cggctgaggg atctgatcgg catcgaggtc gtgctgaccc gggccgaggc
     4261 cgcagcccgc ttcgagctgc cgcccgaccg catcggcgac ctggtggtcg tctccgagcg
     4321 cagcgttgtc ctggggaccg gcaaggcaag acacgacctg tcgcagctcg aactgccgtt
     4381 gcgctcgcat ggaggcattt ccgagcagcg cgttccgatc atcctgaacc gtcgcatcgc
     4441 gggcctggat tcgaaccgtc gctggcgcaa cttcgacgtt ttcgatctcg cgctcaacca
     4501 cgcccggtag cgatgaacgc accgaccctt ccgctgcgcc acgagtcgct gagaatcgcc
     4561 ggcgaaagcg tggccaccga gcgcaacttt gaagtgcgct atccgtacac gggcgaggtc
     4621 gtcgccacgg tgccaaaggc gaccgtcgac gatgtccgtc tggcgattgc atccgcgcgc
     4681 gcctaccggg cccggctgac gcggtacgag cgttaccgca tcctgatgcg cgccggtgag
     4741 ttgatcgctg cccgccgcga cgaaatcgcg cgcacgatca cgctcgaatc cggcctgtgc
     4801 ctgaaggaca cccgctacga agtcggccgt gccagcgacg tgctgctctt cgcggcaaac
     4861 caggcgctgg tcgacgatgg ccaggtcttt tcctgcgacc tgacgcccca tggacgatct
     4921 cgaaaggtct ataccctgcg cgagccgttg caggggatca tcaccgcgat cacgcccttc
     4981 aatcacccac tgaaccaggt gatccacaag gtcgcgcccg cggtggccac caacaatcgc
     5041 gtggttctca aacccagcga gaagacgccg ctggccgcct acatgttggc cgacatcctt
     5101 tacgaggccg ggctgccgcc gccgatgctg tcggtggtga ccggcgatcc gcgcgagatc
     5161 gccgacgaac tgctgaccca tccggacgtc gacctcgtga ctttcaccgg gggtgtggcc
     5221 gtcggcaagg ccatcgccgc caaggcaatt tacaagcgcc aggtgctgga gcttggtggc
     5281 aacgatccga tcatcgtgat ggacgacgcc gacgtcgagg aggccgcgac cctggccgcg
     5341 agcggctcgt acaagaattc cgggcagcgc tgcaccgcgg tcaagcggat gctcgtgcat
     5401 gcgagtgtcg ccgacgactt cgtcgtacgg ctggtcgcaa agtcgaaggc cgtgaagtgc
     5461 ggcgacccgc tcgatccggc gactgacatg ggcaccgtga tcgacgagcc ggccgcagtt
     5521 gagttcgagc gtcgggtcaa cgacgcaatg gacgccggcg cccggctgct gctaggcaac
     5581 gagcggcgcg gagcgctgta cgccccgacc gtgcttgacc acgtccagcc atcgatgagg
     5641 ctggttaagg aagagacgtt cggtccggtg tcgccggtga tccgtttcac gtcgctggac
     5701 gaggcaattg cggtagccaa ttcgactgcc tacggcctgt cgtcgtcggt gtgcaccaat
     5761 cgcctcgacg tcatcacccg atgcattgcc gaactgaacg tcggcaccgt gaacgtgcgc
     5821 gaagtgccgg gttaccggct ggaactgacg cctttcggcg gaatcaagga ctcgggactg
     5881 gggtacaagg aaggcgtaca ggaagcaatg aagagcttca cgaacgtcaa gacctactcc
     5941 ctgccctggt agatctcaat gtcctgtaac gcggccaacg agccgcaacc gaggtaacgg
     6001 ccgcaattga cgacactact gaatctgctg gctgccatcg ccctgctggt ctggggcaca
     6061 cagatcgtgc gcaccggcgt gctgcgcgtg tatggcgccg cgctgcgtca ggtgctgagg
     6121 cgcagcatga atgggcgggc ggccgcgttt ttcgccgggg tcggcgttac cggtttgctg
     6181 cagagttcga ccgccacctc gctgctcacg tcctctttcg tttcgcaggg cctgctgacg
     6241 acatcggccg cgctcgccgt gatgctcggt gccgacgtcg gcacggccgc gatggtgcag
     6301 ctcttctccc tcgatctgtc gtggctggcg ccgctggcga ttgtcgtcgg cgtcgtcgtc
     6361 tatctctcgc gccgtaacga gcgcgccggc cagatcggcc gcattgtgct tggcctcggc
     6421 gtgatgatcc tcgcgctgca actggtcacc gccgccacgg cgcctctggc gcacggcgca
     6481 ggcgtcaaga cattccttgc gcagatttcc ggcgacctgc tgctcgatgt tctcatcgga
     6541 gccgtgttcg cggtgatgtg ctactcgagc ctggccgtgg tcctgctggt ggcaatgctg
     6601 gccggctcga acgtgttgcc ggcacatgtg gccctgccgc tggtgctggg cgccaatctt
     6661 ggcagcggga tcctggcgct gctgaccacc tggcggtcgg atgcggtcat gcgccgtgta
     6721 ccgctcggca atttcgtgtt caaggccgtc ggcgtgacgc t
//
LOCUS       DATIRT010000285         3020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_338718, whole genome shotgun sequence.
ACCESSION   DATIRT010000285 DATIRT010000000
VERSION     DATIRT010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338718"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /gene="hemN"
                     /locus_tag="VLN25_06510"
     CDS             <1..1023
                     /gene="hemN"
                     /locus_tag="VLN25_06510"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008811677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HSQ10249.1"
                     /translation="LSNDELAQLMALLTARFPLAERGEYSIEVDPRSTPPDKVVALGK
                     LGFNRMSVGVQDFEPEVQRAVNRLQSFEMTQATVDAARKADFKSVNLDLIYGLPKQTR
                     ETFARTLDKVMVLSPERIALYHYAHLPDRFKPQRRIETAELPSPEEKLNIMLDAIRRL
                     SDAGYHYIGMDHFAKTGDDLARAQRQGRLHRNFQGYSTRPDCDLIGLGVSAISKIGPT
                     YSQNVRTIEEYYDRLKLGTLPTARGIALDRDDILRRAVIMALMCHFEVSKESIDTTYL
                     IKFDDYFKTEIAELKLFEDEGLVENTPEWITVTPKGKLLVRAVAMTFDRYLRADHRAR
                     RYSKIV"
     gene            1215..1961
                     /locus_tag="VLN25_06515"
     CDS             1215..1961
                     /locus_tag="VLN25_06515"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HSQ10250.1"
                     /translation="MLDLLTTQALAAFTLALLGGLHCAGMCGGFVGALQVHRPHAVPA
                     ARLAAGYHAGRVTSYTLAGALVGTLGGALYAADVLPLQIGLLVVGSLMLLAIGASLVG
                     RAAWLKRLEPLGLGIWRLIAPYARRVYPPRSGSQALLAGLAWGWIPCGMVYAALPLAL
                     VAGGPWQGAAVMLAFGVGTLPNMLAVDVAVTKLGGSGNADHFAGARAWLKTAAGALIV
                     VFGLSGLAHAARIAGAGHPTIEALASICHR"
     gene            2049..>3020
                     /gene="alaS"
                     /locus_tag="VLN25_06520"
     CDS             2049..>3020
                     /gene="alaS"
                     /locus_tag="VLN25_06520"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HSQ10251.1"
                     /translation="MKSAEIRERFLKYFESKGHTIVRASSLVPSNDPTLLFVNSGMVQ
                     FKDVFTGREQRPYKRATTSQKSLRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     RDAIRYSWELLTEVYKLPKDKLWTTVYVDDDEAYDIWTREIGVPAERCIRIGDKAGQP
                     KYVSDNFWQMADTGPCGPCSEIFYDHGPEVAGGPPGSPDADGDRYIEIWNLVFMQFDR
                     DAAGVLTPLPKPCVDNGMGLERLAAVLQHVHSNYEIDLFQNLIKAAARETDTKDLGSN
                     SLKVIADHIRACAFLIADGVIPGNEGRGYVLRRIIRRGLRHGYKLGQT"
BASE COUNT          518 a          973 c         1003 g          526 t
ORIGIN      
        1 ctgtcgaacg atgaactcgc gcagctgatg gccctgctca ccgcgcgctt tccgctggcg
       61 gagcgcggcg agtattcgat cgaggtcgat ccgcgctcga cgccgcccga caaggtggtg
      121 gcactgggca aactgggctt caatcgcatg tcggtcggcg tgcaggactt cgaacccgag
      181 gtgcagcggg cagtcaaccg gctgcagagc ttcgagatga cgcaggcgac ggtcgatgcc
      241 gcacggaagg ccgacttcaa gtcggtcaat ctcgacctga tctacggcct gccgaagcag
      301 acgcgcgaga ccttcgcgcg cacgctcgac aaggtgatgg tgctcagccc cgagcgcatt
      361 gcgctgtatc actatgcgca cctgccggac cgcttcaagc cgcagcgacg catcgaaacc
      421 gctgagctgc cgtcaccgga ggagaagctg aacatcatgc tcgatgcgat ccggcgcctg
      481 agcgatgccg gctaccacta catcggcatg gatcactttg cgaagacggg tgacgacctg
      541 gcacgcgcgc agcgccaggg gcgcttgcac cgcaacttcc agggctattc cacgcgtccg
      601 gactgcgacc tgatcggtct cggtgtgtcg gcgatcagca agattggccc aacgtactcg
      661 cagaacgtac gcacgatcga ggagtactac gatcgcctga agctcggcac tttgcccacg
      721 gcgcgcggca ttgcgctcga tcgcgacgac atcctgcggc gcgcggtcat catggcgctg
      781 atgtgccact tcgaggtctc gaaggagtcg atcgatacga cctacctgat caagttcgac
      841 gactacttca agacggagat cgccgagttg aagctcttcg aggacgaagg gctggtcgag
      901 aacacgcccg aatggatcac ggtgacgccg aagggcaagt tgctggtgcg ggccgtggcg
      961 atgacgttcg atcgctacct gcgtgccgat catcgcgcgc ggcggtacag caaaatcgtc
     1021 tagaggcgct cctcgtgtac gcgaccgctg cgcggtcgca cgtgcactcg gagcggaaca
     1081 gttagtcggc tgcgctccgg agcggccgcg cgcttcgccg aaggtcaact gcaaggcact
     1141 cccccggccc ctcgccgagc gaggggtggt tccggcgcgg cgcgcctcgc cggtcgctgg
     1201 cgcacaatgc ccctatgctg gatttgctga ccacccaggc cctggccgcg ttcacactcg
     1261 ccctgctggg cgggctgcac tgtgccggca tgtgcggagg cttcgtcgga gcgctgcagg
     1321 tgcatcgccc acacgcggtc ccggccgcac gactcgcggc gggctatcac gcgggacgcg
     1381 tcaccagtta cacgcttgcc ggcgcgctcg tcggcacatt gggcggagcg ctttatgcgg
     1441 cggacgtgct tccgttgcag atcggcctgc tggtcgtggg ttcgctgatg ctgctggcga
     1501 tcggcgcatc gctcgtcggg cgggccgcct ggctcaagcg gctcgagccg ctcggcctgg
     1561 gaatctggcg tttgatcgcg ccctacgccc gccgcgtcta tccgcctcgt tccggctcac
     1621 aagcgctgct cgcgggtctg gcgtggggct ggattccctg cgggatggtg tacgcggcgt
     1681 tgccgctggc tctggtcgcg ggtggcccct ggcagggcgc ggcggtgatg ctcgcgttcg
     1741 gcgtcgggac cctgcccaac atgctggcgg tggacgtggc tgtgacaaag ctgggaggca
     1801 gcggcaatgc agatcacttc gccggcgcac gcgcctggct gaaaacggcg gccggcgcgc
     1861 tcatcgttgt cttcggtctg tccgggctcg cacacgcggc gcgcatcgcg ggtgccgggc
     1921 atccgaccat tgaggcgctg gcgtccatct gccaccgctg agaccgcgcg ccggcccgtt
     1981 ggcaacgcgc aagtatcatt cccgcaagtg tcatccagga gcgcggcatc cccgcgccga
     2041 atgcagtcat gaaaagcgct gaaatccgcg aacgattcct gaagtacttc gagtccaagg
     2101 gccacaccat cgtgcgggcc tccagtctcg tgccgtccaa cgatccgacg ctgctgttcg
     2161 tcaacagcgg catggtgcag ttcaaggacg tgttcaccgg ccgtgagcag cggccctaca
     2221 agcgtgcgac cacgtcgcag aaatcgctgc gggcgggcgg caagcacaac gacctcgaaa
     2281 acgtcggcta cacggcgcgg caccacacgt tcttcgagat gctgggcaac ttcagcttcg
     2341 gcgactattt caagcgcgac gcgatccgct actcgtggga gttgctgacc gaggtctaca
     2401 agctgccgaa ggacaagctc tggaccacgg tctacgttga cgatgacgag gcctacgaca
     2461 tctggaccag ggagattgga gtcccggccg agcgctgtat tcgcatcggc gacaaggcgg
     2521 ggcagccgaa gtacgtttcg gacaacttct ggcagatggc ggacaccggt ccttgcgggc
     2581 cgtgctccga aatcttttat gaccacggtc ccgaggtcgc cggcggcccg ccggggtcgc
     2641 cggacgccga tggcgaccgc tacatcgaaa tctggaacct ggtgttcatg cagtttgatc
     2701 gcgacgccgc gggcgtgctg actccgctgc ccaagccgtg cgtcgacaac ggcatgggtc
     2761 tcgagcgcct ggcagccgtg ctgcagcacg tgcactcgaa ctacgagatc gacctcttcc
     2821 agaacctgat caaggcggcg gcgcgcgaga ccgacactaa ggatctcggc agcaattcac
     2881 tgaaggtgat cgccgatcac atccgcgcct gcgcgttcct gattgccgac ggcgtgattc
     2941 cgggcaatga agggcgtggc tatgtgctgc gtcggatcat ccggcgtggg ctgcggcatg
     3001 gctacaagct gggacagacc
//
LOCUS       DATIRT010000286         3531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1715796, whole genome shotgun sequence.
ACCESSION   DATIRT010000286 DATIRT010000000
VERSION     DATIRT010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1715796"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1599)
                     /locus_tag="VLN25_06525"
     CDS             complement(<1..1599)
                     /locus_tag="VLN25_06525"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HSQ10252.1"
                     /translation="MNVTDSSIGKFPGLSRALAPAVRLFGPQTSSAARERRDTLVLLL
                     AVGLVVVPHFEHLAWWATSVVLLLLVWRVWLTLAQRPLPGRFIMLPLLVAAGAAVYLQ
                     HRTLAGQDAGVTFLLLLMALKLLEMRARRDIFVVIFLSFFILLTQFLYGQDLPVAAIT
                     VAAVVALFFVLVSVNLDDVDLPALRKLRLVGITLLKALPLAAALFLLFPRITGPLWGM
                     PGESSEGKTGLSNSMSPGSISQLLESDAVVFRARFENAVPANDKLYWRGPVFGSFNGR
                     SWTPLNRRRADVTVPGIEGEPRSAVNYEVTLEPHKRDWLFALDVPAALPALESLQARL
                     TPEMQLLATGLITERLRYEMRSYTGYRLGARATELELQDWLELPRGFNPRAIQFAAEL
                     RSRVVPAAATQRLSHDSQLVNAVLDHFRRGEFSYTVSPPPLGRHSVDEFLFDTRLGFC
                     EHYASAFVFLMRALDVPARVVTGYQGGELNPVDGFMTVRQADAHAWTEVWLSGRGWVR
                     VDPTAVVAPFRIERGAAELARQASG"
     gene            complement(1686..2699)
                     /locus_tag="VLN25_06530"
     CDS             complement(1686..2699)
                     /locus_tag="VLN25_06530"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HSQ10253.1"
                     /translation="MNMTAHPLTIRIGQWIAGRRGPESGAVVLDRRRVFILPTVPGLV
                     FGAAMLVLLIGSINYSLQLGYVLTFLVTSMAIVGMHTTHANLAQIVLRGIRVEPVFAG
                     DVAVFELTATNPTTVDRFALRFAFIDPAAQPRWFSLRAKRAMAMPSVSTEVPARGDRN
                     VGVPLPAPVRGRLPAPRIVIETRFPFGLWRAWAYLTPALSAMVYPAPEVNAPPLPATG
                     LGGGEGVGLASTGDDFAGVRPYSPGDPQKMIAWRLAARSDELSVKQFEAQGGGELLLD
                     FDGLPRHIDFEHKLSRLARWVLDADAAHMRYGIRLPGSMVFAGTGPKHREHCLTALAM
                     YES"
     gene            complement(2848..3531)
                     /locus_tag="VLN25_06535"
                     /pseudo
     CDS             complement(2848..3531)
                     /locus_tag="VLN25_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669016.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
BASE COUNT          660 a         1154 c         1190 g          527 t
ORIGIN      
        1 gccactggcc tggcgtgcga gttcggcagc gccgcgttca atgcggaagg gcgccacgac
       61 cgcggtcgga tcgacgcgca cccagccgcg acccgagagc cacacttcgg tccacgcgtg
      121 ggcgtccgcc tgccgtaccg tcatgaaacc atcgacaggg ttcagctccc cgccctgata
      181 cccggtgacc acgcgcgccg gcacgtcgag cgcccgcatc aggaagacga acgcagacgc
      241 gtagtgctcg cagaagccca ggcgggtatc gaacaggaac tcgtcgaccg agtgtcgccc
      301 gagtggcggt ggcgacacgg tgtagctgaa ctctccgcga cggaagtgat cgagcacggc
      361 gttcacgagc tggctgtcgt gcgaaaggcg ctgcgtggcc gccgccggaa ccacgcgact
      421 gcgcagttct gccgcgaact gaatcgcgcg cgggttgaaa ccccgcggca actcgagcca
      481 gtcctgcagt tccagctcgg tcgcacgcgc gccgagtcga tagccggtgt acgaacgcat
      541 ctcgtaccgg agtcgctcgg tgatgaggcc ggtggcaagc aattgcatct cgggagtgag
      601 ccgggcctgg agtgattcga gtgccgggag agccgccgga acatcgagcg cgaagagcca
      661 gtcgcgcttg tggggttcga gcgtgacttc gtagttgacc gcggagcgtg gctcgccttc
      721 gatacccggt accgtcacgt cggcgcgtcg ccggttaagc ggcgtccacg agcgtccgtt
      781 gaaggaaccg aacaccggcc cacgccagta gagcttgtcg ttggccggca cggcattttc
      841 gaagcgggcg cgaaagacga cggcgtcgga ctccagcaac tggctgatcg atcccggcga
      901 catcgagttc gatagtcccg tcttgccctc gcttgactcg ccgggcatgc cccacagtgg
      961 gccggtgatg cgcgggaaca gcaggaacag agccgctgcc agtggcaggg ccttcagcag
     1021 agtgattcct accagtcgca gtttgcgcag ggccggcaga tcgacgtcgt ccagattgac
     1081 gctgacaagc acgaagaaca gggccacgac ggcggccacc gtgatcgctg ccaccggcag
     1141 gtcctgcccg tacaggaact gcgtcagcag aatgaaaaac gaaaggaaga taacgacgaa
     1201 gatgtcgcgg cgcgcgcgca tttccagcag cttgagcgcc atcagcagca gaaggaaggt
     1261 cacgccggcg tcctgcccgg cgagcgtgcg atgctgcagg tagacggcag cgccggcggc
     1321 caccaacagc ggcagcatga tgaagcggcc cggaagcggt cgttgcgcca aagtcagcca
     1381 cacgcgccag acgagcagca gcaggacgac cgacgtggcc caccaggcca ggtgttcgaa
     1441 atgcggtacg accaccaggc cgacggccag cagcaggacg agtgtgtcgc ggcgctcgcg
     1501 tgcagcgctc gacgtctgcg gcccgaagag ccgcactgcc ggcgccaacg cacgcgagag
     1561 tccgggaaac ttgccgatgg acgagtcggt gacgttcatg tcacggcccg tctgacgacg
     1621 cagcgattcg ccgtgcccgc ggaatggaag gccagcgcat gcggcgcggg agtgatcggt
     1681 tcatcctagc tttcgtacat cgcgagtgcg gtcagacagt gctcacggtg cttcggcccg
     1741 gtgccggcga agaccatcga cccgggcagc cgtataccgt agcgcatgtg cgcggcatcg
     1801 gcatcgagga cccagcgcgc cagtcgcgat aacttgtgct cgaagtcgat gtggcgcggc
     1861 aggccgtcga agtcgagcag cagctcgccg ccgccctgcg cttcgaactg cttgaccgac
     1921 agctcgtcgc tgcgtgccgc caggcgccag gcgatcatct tttgcggatc gccgggcgag
     1981 taaggccgca cgcccgcgaa atcgtcgccc gtcgatgcca gcccgacgcc ctcgccaccc
     2041 ccgaggcccg tggcaggcag cgggggagca ttgacctccg gcgccggata caccatcgcc
     2101 gacagcgccg gggtcaggta tgcccacgcc cgccacaggc cgaacggaaa ccgtgtctcg
     2161 atgacgatgc gcggagccgg caaccggccg cgtaccggag ccggcagcgg cactccgaca
     2221 ttgcgatctc cgcgcgccgg gacttcggtc gataccgacg gcatagccat cgcacgcttc
     2281 gctcgcagcg aaaaccagcg tggctgcgcg gccggatcga tgaatgcaaa gcgaagcgcg
     2341 aaccggtcga ccgtcgtggg attggtcgcc gtcaactcga acaccgccac gtcgcccgca
     2401 aacaccggtt cgacccggat gccgcgcagc acgatctggg ccagattcgc atgggtggta
     2461 tgcatcccga cgatggccat gctcgtgacc aggaacgtca gcacataacc gagttgcaga
     2521 ctgtagttga tcgagccgat caacaacacc agcatggcgg cgccgaacac gaggccgggc
     2581 accgtcggca ggataaagac gcgccggcga tcgagcacaa ccgcccccga ctcgggacca
     2641 cgacggcccg caatccactg gccgatacgg atggtcaagg gatgcgcggt catattcatc
     2701 agacgacgtt ctcgtaatgc gcgaaaaatt ggcgttgcgg ctgccgcggc gcgttcatgg
     2761 ggctgccctc cgcgctgcgc gttccgggag gagcgcttcg gagcggccgt gcacgctcat
     2821 ggtacgtttt ccggctgctg tcgcttctca cggcaccggg acggcgccga tcagctggct
     2881 gacgagttcc ctgccgtgca gcgctcggcc gctatcgcgg ctgatcgcat gcaggcggtg
     2941 gccggcaacc gcaggcatca cgttctgtac gtcctcagga atcacgtgat tgcgcccgtc
     3001 catccaggcc caggcgcgcg aggcggcagc agcgcgagac cgccgcgcgg tgacaggccc
     3061 tcagcgtact tgccggaggc gcgcgtatga cccatcaggg cctgcacgta gtcgagcagt
     3121 gcggcagaca cgaacacctt cttcacttcg gcctgtgcgg tgacgaggtc actcgggcgc
     3181 gcgactgcgg gcaacgactt cagcatgtcg cgccgcgctt cgcccgacag cagggcgcgt
     3241 tctgcggcgc ggtccgggta gccgaggctg atgcacatca ggaaccggtc gagctgtgat
     3301 tccggcagcg ggaaggttcc cacctgtgac agcggattct gcgtggcgat cacgaaaaat
     3361 ggatgtggca gcggacgtga caggccgtct accgacacct ggctttcctc catggcttcg
     3421 agcatcgcac tctgggtctt ggggctggcg cggttgatct cgtcggccag cagcacttcg
     3481 gtgaagatcg gacccgggtg aaagacgaac tgcccaccgg tgcggtcata g
//
LOCUS       DATIRT010000287         3132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_141561, whole genome shotgun sequence.
ACCESSION   DATIRT010000287 DATIRT010000000
VERSION     DATIRT010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141561"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VLN25_06540"
     CDS             <1..324
                     /locus_tag="VLN25_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HSQ10254.1"
                     /translation="DEAAMLTGSIGMLPSASLNDRKQGLYEPSHGSAPDIAGKGIANP
                     LATILSAAMMLRYSLDRAEQAERIEAAVCKVLAQGLRTADIAVAGASSIGTVAMGDAV
                     VAALA"
     gene            362..1489
                     /gene="asd"
                     /locus_tag="VLN25_06545"
     CDS             362..1489
                     /gene="asd"
                     /locus_tag="VLN25_06545"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HSQ10255.1"
                     /translation="MKLGIVGWRGMVGSVLMDRMSAEKDFELVDTTFFSTSNAGGAAP
                     AVPRAQKSLKNADDIAALTAQDVVITCQGTDYTKEIYPKLRATGWNGYWIDAAKPLRM
                     ADDAVIILDPVNRPVIDAALKRGIRNYIGGNCTVSCMLMGVGALFKQDLVEWMTCTTY
                     QAASGGGAQHMRELLTQFGTLNLEVRDLLADPASAILEIDRRISGKQRALGDDETQHF
                     IVPLAGNLIPWIDADRGDGTSLEEWKGGAEVNKILGRGPGFSAAATPIDSICVRIGAM
                     RCHSQSLTIKLRRDVALTDIEAMIRDDNAWVKLVPNTKDASIRELTPAAVTGTMSIPV
                     GRLRKLAMGADYIGAFTVGDQLLWGAAEPLRRMLRILLGSL"
     gene            1677..>3132
                     /locus_tag="VLN25_06550"
     CDS             1677..>3132
                     /locus_tag="VLN25_06550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HSQ10256.1"
                     /translation="MWCALSLALGLGLSSLEVDAAGLGRLSVQSGLGQPLRAELEVTS
                     VGRDEAPTLAVRLAPLSAFRAANLEFNPALTNVRFVLDRRPDGNYLVRISSAQPVNEP
                     YLDMMVELTWATGRVIREYTVLLDPPSLKVAPDIVAPATPVAPPTATAAAPMATTPRV
                     APPAAAPTPAPAAAASAPTSAPTPKTAPTPSTASGGSYTVKSGDTLGKIANQNRSAAS
                     LDQMLVALFRANPDAFINKNMNLLRAGAQLAIPSDAEAAALTAPESRREVVAQSADFA
                     QYRSRLAQAAGAAPVAAAPSAAAGGGRVTAKVDDKSATAKPGGDQLKIARADGKPTAA
                     AAATAKADEAAAKGRALKEEQERAEQLRKTNEAIQKALEVQSKAGAQAQKKAEQKGAP
                     PSVAPTPAPVPVAAVPAPAPMATAPTPAPATTAVPPPADPAKAEAPKMDTPPAVAPAP
                     APAPVKKATPPPPPPADEPSFIGELLGNTTTQLGL"
BASE COUNT          593 a         1029 c         1037 g          473 t
ORIGIN      
        1 gacgaggcgg ccatgctgac cggatcgatc ggcatgctgc cgtcagcctc gctgaatgac
       61 cgcaagcagg gactgtacga accctcgcac ggcagcgcgc cggacattgc cggcaaaggc
      121 atcgccaatc cgctggcgac gatcctgtcg gcggcgatga tgttgcggta ctcgctggat
      181 cgggcggagc aggccgagcg gatcgaggcg gcggtgtgta aggtgcttgc acaggggctg
      241 cgcacggccg acattgcagt agcaggagcg tcgtcgatag ggacggtcgc aatgggcgac
      301 gcggtcgtcg cggcactggc atgacggaac gagacaggca acgagcgggc gcgaggcgga
      361 aatgaagcta ggcatcgtcg gttggcgcgg catggtggga tcggtcctga tggaccgcat
      421 gagcgccgag aaagacttcg aactggttga cacgacgttt ttcagcacat cgaacgccgg
      481 cggcgcggcg cccgcggtgc cgcgcgcgca aaagtcgctg aagaacgccg atgacatcgc
      541 cgcactgacg gcgcaggacg tcgtcatcac ctgccagggt acggactaca cgaaggagat
      601 ctacccgaag ctgcgcgcga ccggatggaa cggctactgg atcgacgccg ccaagccgct
      661 gcgcatggcc gacgatgccg tgatcatcct cgacccggtc aatcgcccgg tcatcgacgc
      721 ggcgctgaag cgcggaatca ggaactacat cggcggcaac tgcacagtga gctgcatgct
      781 gatgggagtc ggggcgctgt tcaagcagga tctggtcgag tggatgacct gcaccaccta
      841 ccaggccgcg tccggcggcg gcgcgcagca catgcgagag ttgctgacgc agttcggcac
      901 gctcaacctc gaagtgcgcg acctgctggc cgatccggcc agcgcgatcc tcgaaatcga
      961 tcgtcgcatc agtggcaagc aacgcgcgct cggtgacgac gaaacacagc acttcatcgt
     1021 gccactggcg ggcaacctga tcccttggat cgatgccgat cgcggcgacg gcaccagcct
     1081 cgaagagtgg aagggcggcg ccgaggtcaa caagatcctg ggccgcggtc cgggattcag
     1141 tgccgccgca acgccgatcg actcgatctg cgtgcggatc ggcgccatgc gctgccactc
     1201 gcagtcgctg acgatcaaac taaggcgcga cgtagccctg actgacatcg aagcgatgat
     1261 ccgcgacgac aatgcgtggg tgaaactcgt gcccaacacg aaggacgcga gcattcgcga
     1321 actcacgcct gccgcagtca ccggcacgat gtcaataccc gtcgggcggc tgcgcaagct
     1381 tgcgatgggt gccgattaca tcggtgcgtt caccgtcggc gatcagttgc tctggggcgc
     1441 ggccgagccg ctgcgccgca tgctgcgaat cctgctcgga tcgttgtaaa gcggcaaacg
     1501 tccggcgcgg cggcgctggt tgcgtgcatg actcgcgccg agtttcattt ctcacataaa
     1561 ggcgtgagtt acggctattt ctaagcttgc cgccctaagt ccatgatgtt atgttcgaaa
     1621 aacgtttcaa taagacaggg ggcttaatgc catcccataa attttcgcgc gcgcccatgt
     1681 ggtgcgcgct ttcgcttgcg ctaggccttg ggctgtccag cctggaggtc gacgcagcgg
     1741 ggcttggccg cctgtcggtg caatcggggc tcggccaacc gctgcgagcc gagctggaag
     1801 tcacgtccgt cggtcgggac gaggcgccga ccttggcggt ccgactggca cccctgtcag
     1861 cattccgggc ggcaaatctg gaattcaatc cggcacttac gaacgtccgc ttcgtgctgg
     1921 accggcgccc cgacggcaac tacctggttc gcatctcatc ggcgcaaccg gtcaacgagc
     1981 cctacctcga catgatggtc gagctgacct gggccacggg tcgggtcatc cgcgagtaca
     2041 ccgttcttct ggatccgccg tcgctgaagg ttgcgccgga tatcgttgct ccggccacgc
     2101 cggtggcacc acccaccgca acggcggcgg ctccgatggc gactacgccg cgcgtggcac
     2161 ctccggcagc ggcgccgact cccgccccgg ccgcggcggc ctccgccccc acgtccgcgc
     2221 caacaccgaa gactgcgccg accccgtcga ccgcctccgg tgggagctat acggtcaagt
     2281 ccggtgacac gcttggcaag attgccaacc agaaccgcag cgcggcctcg cttgaccaga
     2341 tgctggttgc gctgttccgc gccaatcccg acgcgttcat caataagaac atgaatctgc
     2401 tgcgcgccgg agcgcagctg gccattcctt ctgacgccga ggcggccgca ttgacggcgc
     2461 ctgaatcgcg tcgcgaggtg gtggcgcaga gtgccgactt tgcccagtac cgcagccggc
     2521 tggcacaggc tgccggtgct gcgccggtcg cagccgcacc gtcggccgcc gcaggcggtg
     2581 gacgcgtcac cgcgaaggtc gatgacaaga gtgccaccgc caaaccgggt ggcgaccagc
     2641 tgaagatcgc ccgtgccgac ggcaagccaa cggcggctgc ggccgcgacg gccaaggccg
     2701 atgaggctgc tgcgaagggt cgtgcgctga aggaagaaca ggagcgcgcg gaacagctca
     2761 ggaagaccaa cgaagcgatc cagaaggcgc tggaagtgca gagcaaggcc ggcgcccagg
     2821 cacagaagaa ggccgagcaa aagggtgctc ctccgtcggt cgcgcctact cctgcgccgg
     2881 taccggtggc ggcggtcccc gctcccgcgc cgatggcgac tgcaccgact ccagcacctg
     2941 cgaccaccgc ggtacctccg ccagcagatc cggccaaggc cgaagcaccg aagatggaca
     3001 cgccgcccgc agtggcaccc gctccggcgc cggcacctgt gaagaaagcg acaccgccgc
     3061 cacccccgcc ggccgacgag ccgagcttta tcggcgaact gctcggaaac acgacaacgc
     3121 agctcggact cg
//
LOCUS       DATIRT010000288         2649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1322113, whole genome shotgun sequence.
ACCESSION   DATIRT010000288 DATIRT010000000
VERSION     DATIRT010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322113"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1607)
                     /locus_tag="VLN25_06555"
     CDS             complement(<1..1607)
                     /locus_tag="VLN25_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HSQ10257.1"
                     /translation="MRMSIEGLVKALSKLAFAGLATCAALAGAQTPAADASNAIERVD
                     ATQTPTGIVLTIDMKNAVQGTPASFSVANPARVALDLPGAVNAVGRNLVEINQGDLRS
                     VNIVQAQGRSRVVLNLKRPLTHAVSVDGKRILVALGGATDTTTFRPTGAAAAAAAAPE
                     ATAAAATPSSGYTAAAPVSGRALRGVDFRRGAEGEGRVIIDLSDPNTNVDIRQQGNTV
                     VVDFLNTTLPDSLKRRLDVSDFATPVNNLSASQQGSTARLIVEPRGLWEHNAYQSDTR
                     FVLEVRPVKEDPQRLFQGTRQGYQGERLSLNFQNVDVRSLLQVIADFTNLNIITSDSV
                     GGTITLRLKDVPWDQALDIILQSKGLDMRKNGNVLLVAPRDEIATKEKLELEARNQIQ
                     ELEPLRSEAFVVNYQKADEVRRLLVDDKQRLLSKRGSVAVDPRTNQLFVQDTVARLED
                     VRRLLQRIDIPVPQVLIEARIVEASDDFSRSLGVRLGGNTGYPQQIGGTGAYGSVGGL
                     GPSIATGPGGTVTTNTSASGGPGGSSTT"
     gene            complement(1604..2173)
                     /locus_tag="VLN25_06560"
     CDS             complement(1604..2173)
                     /locus_tag="VLN25_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HSQ10258.1"
                     /translation="MMDLTQPMRLAALVVAVSSLAACGGGSESELRGWMDDTRKAMRP
                     TTQPVPEPKQFSPFTYEAKALIDPFDAQKMVLAVARQAQTRASTSAIKPDLDRRREAL
                     EGFPLDQLKMVGTLRQQGNNVALIDASGQTFMVKAGNYIGQNFGLVTKISETEVDLKE
                     IVQDAAGEWVERPTKLELQEAAARQGSKR"
     gene            complement(2170..>2649)
                     /locus_tag="VLN25_06565"
     CDS             complement(2170..>2649)
                     /locus_tag="VLN25_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HSQ10259.1"
                     /translation="LQAEELKLKDEYKNKLQQAINLDELRKQREQVSQYVLTLEKQLP
                     SKSEMDALLSDINQAGVGRGLDFELFKPGQTNVKEYYAELPIAIKLAGKYHDLGAFAS
                     DIANLPRIVTLNNLNVAAGKSGALTLDATAKTYRYLDADELANQRKAKQAATKGPAK"
BASE COUNT          414 a          855 c          837 g          543 t
ORIGIN      
        1 gtcgtgctcg atcctcccgg gccaccggag gcgctggtgt tggtcgtcac cgttcccccc
       61 gggccggtcg cgatgctggg tccgaggccg ccgaccgagc cataggcacc agtgccgccg
      121 atttgctgcg ggtaacccgt attgccgcca aggcgcacgc cgagcgaccg cgagaaatcg
      181 tcgctggcct cgacaattcg agcctcgatc agtacctggg gcaccgggat atcgatgcgc
      241 tgtagtagcc gtcgcacatc ttccaatcgc gccaccgtgt cctgcacgaa aagctggttc
      301 gtgcggggat cgaccgcaac actgccgcgc ttggacagca ggcgctgctt gtcgtcgacc
      361 agcaggcggc ggacctcgtc agccttttga tagttgacga cgaaagcttc ggaccgcagc
      421 ggctcgagtt cctggatctg gttacgtgct tcgagctcga gcttttcctt ggtcgcaatc
      481 tcgtcacgcg gcgccaccaa gagaacgttg ccgttcttgc gcatgtccag gcccttggac
      541 tgcaggatga tgtcgagagc ctggtcccac gggacgtcct tcagccgaag cgtgatcgtg
      601 ccgccgacgg aatcgctggt gatgatgttc agattggtga agtcagcaat gacctgcaac
      661 agcgatcgca cgtcgacatt ctggaagttg agtgacagtc gttcgccctg gtagccctgg
      721 cgcgtgccct ggaacaggcg ctgcggatct tccttgaccg gccgaacctc gagcacgaag
      781 cgcgtgtcgc tctggtaggc attgtgttcc cacaggccgc gcggctcgac gatcaggcgc
      841 gccgtgctcc cctgctggct cgccgacagg ttgttgaccg gcgtggcgaa gtcggaaaca
      901 tcgagacgcc gcttgagcga gtcgggcaac gtcgtgttca ggaagtcgac aacgacggta
      961 ttgccctgct ggcggatatc gacgttcgtg ttcggatcgg acaggtcgat gattacccgg
     1021 ccttctccct cggcaccgcg acggaaatca acaccgcgca gtgcacggcc gctcaccggt
     1081 gccgccgccg tatagcctga tgaaggcgtt gcggcggccg cggtcgcctc gggtgccgcc
     1141 gcagccgctg ctgctgcacc ggtcggacgg aaggtcgtcg tatcggttgc accgcccagc
     1201 gcgacaagaa tgcgcttgcc atcgacgctg accgcgtgcg tgagcggacg tttcaggttc
     1261 agcacgaccc gcgagcggcc ctgcgcctgc acgatgttca ccgagcgcag gtcaccctgg
     1321 ttgatttcga ccagattgcg gccaactgcg ttgactgccc ccggcagatc gagcgcgacc
     1381 cgagccgggt tggccaccga gaagctggcc ggcgtgccct ggaccgcatt tttcatgtcg
     1441 atggtcagca cgatgccggt aggcgtctgc gttgcatcca cgcgctcgat cgcgttactg
     1501 gcatccgccg ccggggtttg cgcacccgcc aacgcggcgc atgtggccag gccggcgaac
     1561 gccagcttag acagcgcctt gacgagaccc tcgatcgaca tcctcatctc ttgctccctt
     1621 gccgcgcggc ggcctcctgc agctccagct tcgtcggtcg ttcgacccac tcaccggcgg
     1681 catcctggac gatttccttg agatccactt ctgtttccga aattttcgtg acgagcccga
     1741 agttctggcc tatgtagtta cccgctttca ccatgaaggt ctggcccgag gcgtcgatca
     1801 gcgcgacatt gttgccctgc tgccggagcg tgccgaccat cttgagctgg tcgagcggga
     1861 atccctcaag cgcctcgcga cggcggtcga ggtccggctt gatggcgctg gtgctggcgc
     1921 gtgtctgcgc ctggcgtgcc acggcaagaa ccattttctg cgcgtcgaag gggtcgatca
     1981 gcgccttcgc ctcatacgtg aagggtgaaa attgctttgg ctcgggcacg ggctgcgtcg
     2041 tcggccgcat ggccttgcgc gtgtcgtcca tccagccacg caattcgctt tcgctgccac
     2101 cgccacaggc ggcaaggctg gagacggcga cgaccagcgc cgccagacgc atcggttggg
     2161 tcaggtccat cattttgccg gccccttcgt ggccgcctgc ttcgccttgc gctgattggc
     2221 gagttcgtcc gcatcgaggt agcgataggt cttcgccgtg gcatccagcg tcagcgcgcc
     2281 gctcttgccc gccgcgacat tcaggttatt cagcgtgacg atgcgcggca agttggcgat
     2341 gtcgctcgcg aaggcaccga ggtcgtggta cttgccggcg agcttgatcg cgatcggcag
     2401 ctcggcgtag tactccttga cgttggtctg gccgggcttg aagagttcaa agtccagtcc
     2461 gcggccgacg ccggcctggt tgatatcgga cagcagtgcg tccatctcgg acttgctcgg
     2521 caactgcttc tccagcgtca aaacatactg gctgacctgc tcgcgctgct tgcgcagttc
     2581 gtcgaggttg atcgcctgct gcagcttgtt cttgtactcg tccttcagct tcagctcttc
     2641 ggcctgcag
//
LOCUS       DATIRT010000289         3081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_339197, whole genome shotgun sequence.
ACCESSION   DATIRT010000289 DATIRT010000000
VERSION     DATIRT010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339197"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..602)
                     /locus_tag="VLN25_06570"
     CDS             complement(57..602)
                     /locus_tag="VLN25_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSQ10260.1"
                     /translation="MDIIVLRGDRVVLRGWERRDAEPFAAMNADPVVMEHFTTALKRG
                     DSDAMMERLQQGLESRGWGAWCLDIDGACAGFVGITPVSFEAPFTPCVEVGWRLAAAY
                     WGKGYATEAARLAVGVGFGTVGLQEIVSFTAVANWRSRRVMERLGMRRNAREDFDHPR
                     VPQGHPLQRHVLYRLAARDFE"
     gene            complement(602..1846)
                     /locus_tag="VLN25_06575"
     CDS             complement(602..1846)
                     /locus_tag="VLN25_06575"
                     /inference="COORDINATES: protein motif:HMM:NF019285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HSQ10261.1"
                     /translation="MQSLQSALGLVVMLAVAWIASEQRRAVPWRIIVSGLLLAFALAA
                     ALLKIPVLAAALSSLNSALAVFEHALQAGTGFVFGYLGGAEPPYAVTKASSNFVLAFR
                     ALPLVLLVAALSALLFHWRVLPLLVRAFAWLLHRTLGIGGSVGVSAAANVFVGMVEAP
                     LVIAPYLGRLSRGELFIVMNCGMATIAGTVLVLYATFLNPVVPNAFGHLLVSSIVATP
                     LAIVVAAIMVPPRLTDDARVSLPREDANAVAALTRGTLEGLQLLLNIVAMLVVFVALV
                     ALANALLGALPDIHGEPLTLERMFGWLFAPLAWLIGIPASEAMTAGALLGKKTVLNEL
                     IAYVDMARLPADALSDRSRLLLTYALCGFANFGSLGIMLGGLSALLPAARRAELAELG
                     MKSIFAGLLATCLTAAIVGVLT"
     gene            1934..>3081
                     /locus_tag="VLN25_06580"
     CDS             1934..>3081
                     /locus_tag="VLN25_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSQ10262.1"
                     /translation="MSPLDMPAALHGVYQRQRASYDAAPFPEWEVRRDRLRRLQRLIG
                     DNERDIEATISADFGTRPRMETQIAEIFPSLAEIGDAVRSGPRWMKPRGAWVSKWFLP
                     ARAHVMPRPLGVVGIIVPWNYPLFLAAGPLAGALAAGNRAMVKMSEYTPAFSQLFADL
                     VAKTFEAEELAVITGGPDVAAQFSALPFDHLLFTGSTVVGRQVMAAAAQNLTPVTLEL
                     GGKSPAVVAPGYPIQRAAQRVLAGKLLNAGQTCIAPDYVLVPRQSVTEFVDATRDQAR
                     RMYPRGILDPDYCSVVNQRHYGRLVGYLDEAIAAGVVVHTLFDGPDRNDEVHRLAPAV
                     LVDPPRTLDVMRSEIFGPLLPVIGYDKVDQAVAFVNAQPRPLALYWFD"
BASE COUNT          584 a         1015 c          992 g          490 t
ORIGIN      
        1 tagataagcc ggcaatctgg cgggttcagc gcaccgttcc ggcatgacct gagaattcac
       61 tcgaagtcgc gagcggccag tcgatacagg acatgtcgct gcaacgggtg cccttgcggc
      121 acccgcggat gatcaaaatc ctctcgcgcg ttgcgtcgca tacccagccg ttccatcacc
      181 cgacgtgaac gccagttcgc gaccgccgtg aacgagacga tctcctgaag gccgaccgtc
      241 ccgaaaccta ctccgaccgc cagccgcgcg gcctcggtgg cgtatccctt gccccagtaa
      301 gccgcggcca ggcgccagcc gacttccacg caaggggtga atggcgcctc gaaagacaca
      361 ggggtgattc cgacgaaccc ggcgcaggcc ccgtcgatgt caaggcacca ggccccccat
      421 ccccggctct cgagtccctg ctgcaatcgt tccatcatgg cgtcactgtc gcctcgtttg
      481 agagcggtcg tgaaatgctc catcacgacc gggtcggcgt tcattgcggc aaacggttcc
      541 gcatcgcgtc gctcccagcc ccgcaggacg acccgatcgc cacgcaggac gatgatgtcc
      601 atcaggtgag tacgccgacg atcgcggcgg tcaggcaggt ggccagcagc ccggcgaaga
      661 tcgacttcat gcccagttcg gccagctcgg cgcgtcgcgc tgccggcagc agtgccgaca
      721 ggccgccgag catgatgccg aggctgccga agttcgcgaa cccgcacagg gcgtaagtga
      781 gcagcaggcg gctgcggtcg gacaacgcat cggccggcag ccgggccatg tcgacatagg
      841 cgataagttc gttgagcacg gtcttcttgc cgaggagggc gccggcggtc atcgcctcgg
      901 aggcgggtat gccgatgagc catgccagcg gcgcaaagag ccagccgaac atgcgctcga
      961 gcgtcaaggg ttcgccatgg atgtccggca atgcaccgag cagcgcgttg gccagcgcca
     1021 cgagggcaac gaatacgacc agcatggcaa cgatgttcaa cagcagttgc agtccctcca
     1081 gcgttccccg cgtcagcgcc gcgacggcat tggcgtcctc gcgcggcagg gacacgcggg
     1141 catcgtcggt cagccggggc ggcaccatga ttgccgcgac gacgatggct agcggggtgg
     1201 cgacgatcga cgagaccagc aggtgcccaa acgcgttcgg aaccactgga ttcagaaacg
     1261 tcgcatagag caccagcacg gtgccggcaa tggtggccat gccgcaattc atcacgatga
     1321 agagctctcc tcgcgacagc ctacccagat agggcgcgat caccagagga gcctcgacca
     1381 tgccgacgaa gacgttggcc gcagccgaga ctccgaccga tcctccgatg cccagggtcc
     1441 gatgcagtag ccaggcaaag gcacgcacca gcagcggcag cacgcgccag tgaaaaagca
     1501 gcgctgacag cgccgccacc agcagcacca gaggcagtgc gcgaaaggcc aacacgaagt
     1561 tcgagcttgc ctttgtcacc gcatacggtg gctcggcgcc gccgaggtaa ccgaaaacaa
     1621 aacccgttcc tgcctgaagt gcgtgctcga agacagcgag cgcggaattc aggctcgaaa
     1681 gggcggctgc aagtacggga atcttgagca gagcggctgc gagtgcgaac gcgagcagca
     1741 gaccgctgac gatgattcgc cacgggaccg ctcgacgctg ctcggacgcg atccaggcca
     1801 cggccagcat cacaaccagg ccgagtgcgc tctgcaggct ctgcattccg ctccctcccc
     1861 ggagcaccga cattggagca caggctcgat cctgccgcag taagcttgaa tatcttctat
     1921 cgccggattg cgtatgagtc ccttggatat gccggccgcg cttcacggcg tgtaccagcg
     1981 ccagcgcgcg agctacgacg ccgcgccctt tcccgagtgg gaagtgcggc gcgatcggct
     2041 gcggcgcctg cagcgactca tcggcgacaa cgagcgcgac atcgaggcga ccatcagtgc
     2101 ggacttcgga acgcgtccaa ggatggaaac gcagattgcc gagatcttcc cgagcctggc
     2161 ggagatcggt gacgctgtgc gcagcggacc gcggtggatg aagccccgcg gggcctgggt
     2221 atcgaagtgg tttcttccgg cgcgcgctca tgtgatgccg cgcccgctcg gcgtggtcgg
     2281 catcatcgtg ccgtggaact atccgctgtt cttggccgca ggcccgctcg cgggtgcact
     2341 ggctgcgggc aaccgagcca tggtcaagat gtcggagtac acgccagcct tctcacagtt
     2401 gtttgccgat ctcgttgcca agaccttcga ggcggaagag cttgccgtca tcaccggggg
     2461 tccggacgtc gcggcccagt tctctgcgct gcccttcgac catctgctct tcacgggctc
     2521 gacggttgtc ggccggcagg tgatggcggc tgccgcgcag aacctgaccc cggtgactct
     2581 tgaacttggc ggcaagtctc cggccgtcgt ggcgccgggc tatccgatcc agcgcgccgc
     2641 gcaacgcgtg ctggctggca agctgctgaa cgcagggcag acatgcatcg cgccggacta
     2701 cgtgctggtg ccgcggcaaa gcgtgaccga attcgtcgat gccacgcgtg accaggcacg
     2761 acgcatgtat ccgcgcggga tactcgatcc ggactattgc agcgtcgtca accagaggca
     2821 ctacggccgc cttgtgggct atctcgatga ggccatcgcg gccggcgtcg tggttcacac
     2881 actgttcgat ggaccggacc gaaacgacga agtgcatcgg ctcgcgcccg ccgtgctcgt
     2941 cgatccgccg cgcacgctcg acgtgatgcg tagcgaaatc tttggcccct tgttgccggt
     3001 catcgggtac gacaaagtcg accaggcggt ggccttcgtg aacgcgcagc cgcgcccgct
     3061 ggcgttgtac tggttcgaca a
//
LOCUS       DATIRT010000290         7728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_339550, whole genome shotgun sequence.
ACCESSION   DATIRT010000290 DATIRT010000000
VERSION     DATIRT010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7728
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339550"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..2723)
                     /gene="mutS"
                     /locus_tag="VLN25_06585"
     CDS             complement(123..2723)
                     /gene="mutS"
                     /locus_tag="VLN25_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HSQ10263.1"
                     /translation="MTARANGKQHTPMMAQYLRIKADHPDVLLFYRMGDFYELFYDDA
                     ERAARLLNITLTARGASAGTPVKMAGVPVVSVEQYLARLVRLGESVAICEQIGDPATS
                     KGPVERKVMRVVTPGTLTDTGLLQDKADAPLLAVAPPLQRGGPFGLAWLVLSSGELRA
                     ASIAPALLSSELARIAPSEVLVADAWSDTLSVTVRNAGAIAQARPDWHFDAVRGAESL
                     QQLLQVVTLDAFAVTDAPEILAAAAALLDYARSTQGGRLPHVASLHREAPSDYLLLDA
                     VTRRNLEITETLRGEDGPTLFRLLDHCETGMGSRRLRHWLHHPLHGDDVPRARHRSIA
                     ALLDGTALHDSLRNALHGLPDLERISTRITLRSVRPRELAALRDSLPAVGRVVELLAA
                     GDAPLLEELRTQCRVPADIAEQLAKALHAEPAHAVRDGDVIRSGHDAELDELRALRDN
                     TGQFLVDLEQRERTRTGISNLRVEYNRVHGFYIEVSRGQSAKVPDDYRRRQTLKNAER
                     YITPELKSFEDRALSARERALAREKTLYEELVEGLARHVPALQAAADALAALDALAAL
                     AHHARLARWVEPELVERAGLEIRGARHAVVEREIDSYVPNDCVLHAGRRLLVITGPNM
                     GGKSTYMRSIALIALLAYAGSFVPAASARLGPVDRILTRIGAADDLARGRSTFMVEMT
                     EAAAILNSATDTSLVLMDEIGRGTSTFDGMALAAAIARELVERNRCLTLFATHYFELT
                     QLAEQLAEVANVHVAAAESNGRVVFLHEVRAGPASQSYGLAVAQLAGVPGSVVRRARG
                     LLTQLEERALGTRPQLDMFAAAPESSAPLPDLDGPLRARLVGIDLDALSPREAHTLLD
                     ELQRQARGAD"
     gene            complement(2793..3587)
                     /locus_tag="VLN25_06590"
     CDS             complement(2793..3587)
                     /locus_tag="VLN25_06590"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HSQ10264.1"
                     /translation="MHPMLNTAVKAARKAGSVINRASLDLEGVRVGTKGRSDFVTEVD
                     HAAERAIVDILLKAYPDHAILAEESGASGQSEYTWIIDPLDGTTNFIHGFPQYAVSIA
                     LRHRDLITQAVVYDPTRNELFTATRGRGAFLNERRMRVSRRDRLAESLIGTGFPFRSF
                     ERLDEYVRMFKSITEHTAGIRRPGAAALDLAYVAAGRLDGFWEIGLSPWDMAAGSLLI
                     LEAGGLVSNFDGDPGYLEDGHIVCGAPKIFPPLLHLVRQVHSGRAA"
     gene            3666..4463
                     /locus_tag="VLN25_06595"
     CDS             3666..4463
                     /locus_tag="VLN25_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HSQ10265.1"
                     /translation="MADSPTLPFDRVRFVLVEPTLAANIGAAARAIKTMGFAHLVVVS
                     PRDVNFRTDADAVARSTGAADVLASSTHAASLTEALQGVGYAIAMTGYDREFGPPLQD
                     LRSVAAGTADQLAALPSGDVAWVFGTERSGLENSDVLRCQVCCAIPANPEFASLNLAQ
                     SVQVAAYECQLALRASAAIQQGQRRFNDDDPPASVEALEKLYEHFEQAATVVGFHDPA
                     EPKTLMPRFRRMLNRARPTQTEVDLLRGVLAAVIEARADRAGRKRRA"
     gene            4584..5342
                     /gene="cysE"
                     /locus_tag="VLN25_06600"
     CDS             4584..5342
                     /gene="cysE"
                     /locus_tag="VLN25_06600"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HSQ10266.1"
                     /translation="MFTRLREDIDCILERDPAASSRWEVLTCYPGLHALALHRLAHAC
                     WKGGLRWLARFVSHVSRWLTGIEIHPGATIGRFVFIDHGMGIVIGETAEIGDGTTIYQ
                     GVTLGGTSLTKGAKRHPTLGRNVIVGAGAKVLGGFTVGDGARIGSNAVVVKDVPAGAT
                     AVGNPARILHRELDAQREETANQLGFSAYGITQEGDDPLSKALHGLIDHATAQQREIE
                     RLVAALERNGIALDDAKREAPKVDPEQLNRLVDE"
     gene            complement(5365..6162)
                     /locus_tag="VLN25_06605"
     CDS             complement(5365..6162)
                     /locus_tag="VLN25_06605"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HSQ10267.1"
                     /translation="MSNSAPATPPALAVERTIDPLVDPMVISDLHLTTEHPRTVERFL
                     RFMREDAPAHRELVILGDLFDFWIGDDAGAAAAPVIAALAAASSAGRRVLVMHGNRDL
                     LLGRDFARATGSVLLADPIVVDIAGKRTLLSHGDAWCTLDVAYQTFRAMVRQPEFQRE
                     FLAKPAAERIAVARRARMQSETEKAVKAMDIMDVTPAAIDSALAAAGVDRVIHGHTHR
                     PAAHVIDLNGRTAERWVLPDWDLDDAEPRGGFIDFVDGRPRLIFFDD"
     gene            complement(6170..6664)
                     /locus_tag="VLN25_06610"
     CDS             complement(6170..6664)
                     /locus_tag="VLN25_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSQ10268.1"
                     /translation="MIRMYTSLGPFTIELDHERAPKTAENFESYVRNGFYDGTIFHRV
                     INGFMVQGGGFETGMRQKTTEAPIENEARNGLRNAKYTLAMARTGDPHSATAQFFINV
                     ADNDFLNYTESTPQGWGYCVFGKVTEGFPVVDKIKAVRTGRRGSHADVPEVDVLIERA
                     EIIQ"
     gene            complement(6681..7223)
                     /locus_tag="VLN25_06615"
     CDS             complement(6681..7223)
                     /locus_tag="VLN25_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSQ10269.1"
                     /translation="MNLLFCLLLSLPGAAWAQRVALDTTQGTIVIELAPQEAPKTVAN
                     FLEYVKSGHYNNTVFHRVMATFMIQGGGYTADLTEKPTRAPIPLESQNGLKNTRGAVS
                     MARRGDPNSATSQFFINVVDNPRLDYPNPDGNGYAVFGKVVEGMDVVDKIRAVPTGAS
                     GSHQSVPTTSVVIKSARIVK"
     gene            complement(7267..>7728)
                     /locus_tag="VLN25_06620"
     CDS             complement(7267..>7728)
                     /locus_tag="VLN25_06620"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSQ10270.1"
                     /translation="ELLTELQQVQQLLAAGRAPQALARADAQLAKNPRDAQMRFVRGV
                     ILTEMKKPAEARDAFERLTEDYPELPEPYNNLAVLYASEGQLESARAALEKALAAAPN
                     YGTAYENLGDIYLQMAADAYQRAFKLDPANRQASAKLTLSRELITKARNVR"
BASE COUNT         1244 a         2501 c         2651 g         1332 t
ORIGIN      
        1 cattgcgtcc tcatgtgcca atgagcgcca cgcgtgttgg catctagcca acggcgctcg
       61 cggcgcctag cactgcctcc cgccattggc cttcgcactc ccggctttgg cgtgaacagg
      121 ccctagtcgg ccccgcgcgc ctggcgctgc aactcgtcca gcagcgtgtg cgcctcgcgc
      181 ggcgagagcg cgtcgagatc gattccgacc aggcgcgcgc gcagcgggcc gtcgagatcg
      241 ggcagcggcg cggacgattc cggcgccgcc gcgaacatgt cgagctgcgg tcgggtgccg
      301 agggcgcgtt cctcgagttg ggtcagcaaa ccgcgggcgc gtcgcacgac cgatcccggc
      361 acacccgcca gctgggccac cgccaggccg tagctctggc tggccggacc ggcccgcact
      421 tcgtgcagga acaccacgcg tccgttcgac tcggcggcgg cgacgtgcac gttcgcgacc
      481 tcggcaagct gctcggcaag ctgcgtcagc tcgaagtagt gcgtcgcgaa caaggtcagg
      541 cagcggttcc gctcgaccag ttcgcgcgcg atcgcggcag cgagcgccat gccgtcgaac
      601 gtcgacgtac cgcggccgat ctcgtccatc agcaccagac tggtatcagt cgcgctgttg
      661 aggatcgcgg ccgcttccgt catttccacc atgaaggtcg agcggccgcg cgccaggtcg
      721 tcggccgccc cgatccgggt caggatccga tcgaccggac cgagccgtgc gctcgcggcg
      781 ggtacgaaac ttcccgcgta ggcgagcaat gcgatcagcg caatcgagcg catgtaggtg
      841 gacttgccgc ccatgttggg gccggtgatg accagcagcc gccggcccgc gtgcagcacg
      901 caatcgttcg ggacatacga gtcgatctcg cgctcgacga ccgcatggcg tgctccgcga
      961 atctcgaggc cggcgcgctc gacgagttcg ggttcgaccc agcgcgcgag ccgggcatgg
     1021 tgtgctagtg ccgccagggc atccagcgcg gccagtgcgt cggcggctgc ctgtagtgcg
     1081 ggcacatggc gcgcgaggcc ctcgaccagt tcttcgtaaa gtgtcttctc gcgtgccagt
     1141 gcccgctcgc gcgcggacag cgcccggtcc tcgaacgact tgagctcagg cgtgatgtag
     1201 cgctcggcgt tcttcagtgt ctggcgacgc cgatagtcgt cgggcacttt cgcagactgg
     1261 ccgcgcgaaa cctcgatgta gaagccgtgc acgcggttgt actcgacgcg caggttggaa
     1321 atgcccgttc gggtgcgctc gcgctgctcg agatcgacga ggaactggcc cgtgttgtcg
     1381 cgcagggcgc gcagttcgtc gagctcggcg tcgtgcccgg acctgatcac gtcgccgtca
     1441 cgcacggcat gggcgggctc cgcgtgcagc gcctttgcaa gctgttcagc gatgtcggcg
     1501 ggaacccggc actgcgtccg cagctcctcc agcaatggcg cgtcgccagc cgcaagcagt
     1561 tcgaccaccc gaccgaccgc cggcaacgag tcgcgcagcg ccgcgagttc acgcggccgc
     1621 acggatcgca gcgtgatgcg cgtgctgatg cgctcgaggt cgggcaaccc gtgcagcgcg
     1681 ttgcggaggc tgtcgtgcag cgccgttccg tcgagcaggg cggcgatcga acggtgacgt
     1741 gcgcgcggca cgtcgtcacc atgcagcggg tggtgcagcc agtggcgcag gcgacggctg
     1801 cccataccgg tctcgcagtg atccagcagg cgaaacagag tcggtccgtc ctcgccgcgc
     1861 agcgtttcag tgatctccag gttgcggcgg gtcactgcat cgagcaacag gtagtccgag
     1921 ggcgcttcgc gatgcagcga ggccacgtgc ggcagccgtc cgccctgcgt ggatcgcgca
     1981 tagtcgagca gcgcagcggc cgccgcgagg atttcagggg cgtccgtcac ggcgaatgca
     2041 tcgagcgtga cgacctgaag caactgctga agggactccg cgccccggac ggcgtcgaag
     2101 tgccagtctg gccgtgcctg ggcgattgca ccggcgttgc gcacggtcac actgagagtg
     2161 tcggaccagg catcggcgac cagcacttcg gacggagcaa tgcgcgcgag ttcagacgac
     2221 agtagcgccg gggcgatcga cgcggcgcga agttcgcccg acgacagcac aagccaggcg
     2281 agtccaaaag ggccaccgcg ctgcaatggc ggcgccaccg ccaagagcgg ggcgtcggct
     2341 ttgtcctgca gcaggccggt gtcggtcagc gtgcccggcg tgacgacgcg catgaccttg
     2401 cgctcgaccg gccctttgct ggtggcggga tcgccgatct gttcacagat cgccacgctt
     2461 tcgccgagtc ggaccaggcg cgcaaggtat tgctcgaccg ataccaccgg cacgcccgcc
     2521 attttcaccg gcgtaccggc cgaagcgccg cgagcggtca gcgtgatgtt gagcaggcgc
     2581 gcagcgcgct cggcatcgtc atagaacagc tcgtagaagt cgcccatccg gtagaacagc
     2641 agcacgtccg gatggtccgc cttgatgcgc aggtactgcg ccatcatggg cgtgtgctgc
     2701 ttgccgtttg cgcgcgccgt catgctcgtt cggtctgggg acgtacgggc gtgtgggcga
     2761 gccaggcgcg aagcgggagg cgccggcgtg cgtcaggcag cgcggccgct gtgaacctga
     2821 cgcaccaggt gcaggagggg cgggaagatc ttgggggcgc cgcagacgat gtggccgtcc
     2881 tcgaggtagc cgggatcgcc gtcgaaattc gaaaccagtc caccggcttc gaggatcagc
     2941 aggctgccgg cggccatgtc ccagggcgac aggccgattt cccagaaccc gtcgagtcgt
     3001 cctgcggcga cgtaggcgag gtcgagcgcg gctgcgccgg gacgacgaat gccggcagtg
     3061 tgctcggtga tgctcttgaa catccgcacg tactcgtcga gacgctcgaa gctgcggaac
     3121 gggaatcccg tgccgatcag gctttcggcg aggcggtcac gccgcgagac ccgcatgcgt
     3181 cgttcgttga gaaaggcccc acgcccgcgc gtggccgtga acagttcatt gcgcgtcggg
     3241 tcgtagacga ccgcctgtgt gatcaggtcg cggtgccgca gggcaatcga tacggcgtac
     3301 tgcggaaagc cgtggatgaa gttggtcgtg ccatcgagcg gatcgatgat ccaggtgtat
     3361 tcggattgcc cactggcgcc agactcttcc gccaggatcg catggtccgg ataggccttt
     3421 agcagaatgt ccactatcgc acgttcggcc gcgtgatcga cttcggtgac gaagtcgctg
     3481 cggcctttag tgccgacacg gacgccttcg agatcgagac tggcacggtt gatcaccgaa
     3541 ccggccttgc gcgcggcctt cacggccgtg ttgagcatcg gatgcatggt gggcgcgtgc
     3601 ggcaggaccg ccgtcgcgtt tagactgaac catgcagttt agtcgatcac cgcagttcgc
     3661 cgaccgtggc cgattcgccc accttgccgt ttgaccgtgt ccgcttcgtg ctggtcgagc
     3721 cgactctggc agccaacatc ggtgcggctg cgcgggcgat caagacgatg ggtttcgcac
     3781 acctggtcgt cgtgagtccg cgcgacgtga acttccggac agatgccgac gctgttgccc
     3841 gatcgaccgg agccgccgac gtgctggcat cgagtacgca tgccgcctcg ctcaccgagg
     3901 ccttgcaggg tgtcggctac gccatcgcga tgaccggtta cgaccgcgaa tttggtcccc
     3961 cgctgcaaga cctgcgcagt gttgcggccg ggaccgccga tcagctggcg gcgctaccga
     4021 gcggcgacgt tgcgtgggtg tttgggaccg agcgcagtgg cctcgaaaac tctgatgtcc
     4081 tgcgctgcca ggtgtgctgc gccattcccg ccaacccgga gttcgcctcg ctgaatctgg
     4141 cgcagtcggt gcaggtggcg gcctacgagt gccagttggc cctgcgtgcc agcgccgcga
     4201 tccagcaggg acaacggcgc ttcaacgacg acgatccgcc ggccagcgta gaagcgctgg
     4261 aaaagctcta tgagcatttc gagcaggcgg cgacggtggt gggattccat gatccggccg
     4321 agcccaagac cctgatgccg cgttttcgac gcatgctcaa ccgtgcccgg ccgacacaga
     4381 ccgaggtcga tctgctgcgc ggcgtactgg ccgcagtgat cgaagcccga gcggatcggg
     4441 cggggcgcaa gcgccgcgcg tgaccggctg cgttcacggc gccgtgcatg agtcgcttca
     4501 tccggctcgc cgcgttgcaa ccgtacaatc gccgcacatc atctcgacag cccggctcga
     4561 caatccagct cgacaccctg aacatgttca cgcgtctgcg cgaagacatc gattgcatcc
     4621 tggaacgcga ccctgccgca agttcacgct gggaggtcct gacgtgctat ccgggtctgc
     4681 acgcgctggc attgcatcgg ctggcgcatg cgtgctggaa gggcgggctg cgctggctcg
     4741 cacgcttcgt gtcgcatgtc tcgcgttggc tgaccggaat tgagatccac cccggcgcga
     4801 ccatcggccg cttcgtcttc atcgatcacg gcatgggtat cgtcattggc gaaaccgccg
     4861 aaatcggcga cggcacgacc atttaccagg gcgtcacgct cggtggcacc tcactgacca
     4921 agggggccaa gcgacatccc acgctcggcc gcaatgtcat cgtgggggcc ggcgccaagg
     4981 tgcttggcgg tttcacggtc ggcgatggcg cgcgcatcgg gtccaatgcg gtcgtggtca
     5041 aagacgtgcc ggcaggcgcc acagcggtgg gcaaccccgc gcgcatcctg caccgcgagc
     5101 tcgatgccca acgcgaggag accgcgaacc agctcggctt ctcggcctac ggcatcacgc
     5161 aggaaggcga cgatccgctc tccaaggcgc tgcacgggtt gatcgaccac gccaccgcgc
     5221 agcagcgtga gatcgagcgc ctcgtcgcgg ccctggagcg caacggcatt gcgctggacg
     5281 atgccaagcg cgaagcgccg aaggtcgatc cggaacagct caaccggctc gtggacgagt
     5341 agcgcggcgg cgcgctgccc gacgtcagtc gtcgaagaag ataagccgcg gtcggccatc
     5401 gacgaagtcg atgaaaccgc cgcgcggttc ggcgtcgtcg aggtcccagt cgggcaggac
     5461 ccagcgctcg gcggtgcggc cattgaggtc gatgacatgc gcggccggcc ggtgcgtatg
     5521 gccgtggatc actcgatcga cgccggcggc agccagcgca gagtcgatag ccgcaggcgt
     5581 tacgtccatg atgtccatcg ccttgactgc cttttcggtc tcgctctgca tgcgggcgcg
     5641 tcgcgccact gcgatccgtt cggccgccgg cttcgccagg aactcgcgct ggaattccgg
     5701 ttgccggacc atcgcgcgaa acgtctggta ggcgacatcc agcgtgcacc acgcgtcgcc
     5761 atgggatagc agagtgcgct tgccggcgat gtcgacgacg atcggatcgg caagtagaac
     5821 gctgccggtg gcgcgcgcaa agtcgcgtcc cagcaagagg tcgcgattgc cgtgcatcac
     5881 cagcacacgc cggccggcac ttgaagcagc ggccagtgcg gcgatgacgg gggccgccgc
     5941 ggcgcccgcg tcgtctccga tccagaaatc gaacaggtcg cccaggatga ccagctcacg
     6001 gtgagccggc gcatcctcgc gcatgaatcg caggaatcgc tcgaccgtgc gcgggtgctc
     6061 ggtcgtcagg tgcaggtcgg agatgaccat cggatcgacc agcgggtcga tcgtgcgctc
     6121 gaccgcgagc gccggcggcg tcgcgggtgc gctgttgctc aaacggtgct cactggatga
     6181 tttcggcgcg ctcgatcagc acgtcgactt ccggcacatc cgcgtggctg ccgcgccgtc
     6241 cggtgcgcac ggccttgatc ttgtcgacca ccgggaagcc ctcggtgacc ttgccgaaca
     6301 cgcagtagcc ccacccctgc ggcgtcgact ctgtgtaatt gaggaagtcg ttgtcggcga
     6361 cgttgatgaa gaactgcgcg gtggccgagt gcgggtcacc ggtacgcgcc atggccagcg
     6421 tgtacttcgc attgcgcagg ccgttgcgcg cctcgttctc gatcggtgcc tcggtcgtct
     6481 tttgccgcat cccggtctcg aagcccccgc cctggaccat gaagccgttg atgacgcgat
     6541 ggaagatcgt gccgtcgtag aagccattgc ggacatacga ctcgaaattt tcggcggtct
     6601 tgggggcgcg ctcgtggtca agttcgatcg tgaacgggcc gaggctggtg tacatgcgga
     6661 tcatgcgaac tcctgctgga ttacttgacg atgcgggcgg acttgatcac gacggaggtt
     6721 gtcgggacgc tttggtgcga cccgctggca ccggtcggta ccgcacggat cttgtcgacg
     6781 acgtccatgc cctcgaccac tttgccgaag actgcgtagc cgttgccgtc ggggttcgga
     6841 tagtccagcc gcgggttgtc cacgacgttg atgaagaact gcgacgtcgc cgaattggga
     6901 tcgccccgcc gcgccatcga aacggcgccg cgagtgttct tcaggccatt ctggctttcg
     6961 agaggaatcg gggcgcgcgt cggtttttcc gtcaggtcgg ccgtgtagcc accgccctgg
     7021 atcatgaacg ttgccatgac ccgatggaac acggtgttgt tgtagtgccc gctcttgacg
     7081 tactcgagaa agttggcaac ggtcttcggc gcttcctgcg gggccagttc gatgacgatc
     7141 gtgccctgcg tggtgtcgag cgccacgcgc tgggcccacg ccgccccggg cagcgacagc
     7201 aggaggcaga acagcaggtt catgggttgg gtcagaagtt tcatgggaaa tcctcaggac
     7261 atgcgctcaa cggacgttgc gcgccttggt gatgagttcg cgggacaggg tcaacttggc
     7321 gctcgcctgg cgattggcgg ggtcgagctt gaatgcacgc tggtaggcat cggcggccat
     7381 ctgaaggtag atgtctccaa ggttttcgta ggccgtgccg tagttcggag cggccgccag
     7441 tgccttttcg agcgcggcgc gggcgctttc gagctgccct tcgctggcat acagcacggc
     7501 caggttgttg tacggctccg gcagttcggg gtagtcctcg gtcaggcgct cgaacgcatc
     7561 gcgggcttcc gccggcttct tcatttcagt gaggatcact ccgcgcacga agcgcatctg
     7621 cgcatcgcgc ggattcttcg ccagctgcgc atcggcgcgc gccagggcct gcggcgcccg
     7681 gccggccgcc agcagctgct gcacctgctg cagttcggtg agcagctc
//
LOCUS       DATIRT010000291         5671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_142189, whole genome shotgun sequence.
ACCESSION   DATIRT010000291 DATIRT010000000
VERSION     DATIRT010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5671
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142189"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /gene="murA"
                     /locus_tag="VLN25_06625"
     CDS             complement(<1..1013)
                     /gene="murA"
                     /locus_tag="VLN25_06625"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HSQ10271.1"
                     /translation="MDKLKIRGGVSLAGEIPISGAKNAALPILCASLLTADDLIVDNV
                     PQLQDVSTALKLLRQMGVVAEREGSTVRLGAAAVTHPEAPYELVKTMRAAILVLGPLV
                     ARFGRARVSLPGGCAIGARPVDQHIKGLELMGASIHIEGGYLIAEAKRLTGTRIVTDM
                     VTVTGTENLMMAATLANGETVIENAAREPEVGDLANALIAMGARIHGAGTNRIVVQGV
                     DRLHGAQHRVMPDRIETGTFLCAVAAAGGDVLLRGAAPATLDAVLDKLREAGAILECA
                     ADAIRVRMTERPRAVSLRTAPYPEFPTDMQAQFMALDCIADGVGRITETIFENRFMHI
                     QEL"
     gene            complement(1234..1479)
                     /locus_tag="VLN25_06630"
     CDS             complement(1234..1479)
                     /locus_tag="VLN25_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HSQ10272.1"
                     /translation="MDPTPDDVRRYIAAGLECSVLEVDGDGRHFNALIVSPAFEGKLR
                     VARHQLVYAALGERMREEVHALSMRTLTPAEYAATAG"
     gene            complement(1514..2329)
                     /locus_tag="VLN25_06635"
     CDS             complement(1514..2329)
                     /locus_tag="VLN25_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSQ10273.1"
                     /translation="MNAPATLREGATISAADSSSAIAFRTLLTKEMLRFWKVSFQTIA
                     APVLTAVLYLLVFGQVLADHVEVFPGISYVAFLIPGLVMMSVLQNAFANSSSSLIHSK
                     IMGSLVFVLLPPFSHWQLFGAYVLASIVRGLVVGLGVFVVAVWFGPPSFKAPLWILVF
                     ALAGSAMLGSLGVIAGIWADKFDQLAAFQNFVVMPATFLSGVFYSVHGLPAFWQRVSH
                     LNPFFYMIDGFRYGFFGQSDVSPWVSLAVVAGALVLTAAIALRLLERGYKLRH"
     gene            complement(2326..3255)
                     /locus_tag="VLN25_06640"
     CDS             complement(2326..3255)
                     /locus_tag="VLN25_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSQ10274.1"
                     /translation="MTAAIQVNSVSKRYGSLVALDHVDLVINEGEFFGLLGPNGAGKT
                     TLISIIAGLVRASSGRVAIMGHDVVDDFRAARLSVGVVPQELAFDPFFTVRETLAFQS
                     GYFGLRGNDAWIDEVLENLGLATKADANTRALSGGMKRRLLVAQALVHKPPVIVLDEP
                     TAGVDVELRQTLWDFIRRLNREGHTIVLTTHYLEEAQALCGRIAMLKGGRIVALDTTR
                     ALLARFAGLQLTMRPRGTPPASVLAHRIDERDGVLTLRLAHSVDVGQVLADCAAAGCI
                     VEDLEVGRADLEDVFLQMMSDKPAQRMAAGTPA"
     gene            complement(3368..3640)
                     /locus_tag="VLN25_06645"
     CDS             complement(3368..3640)
                     /locus_tag="VLN25_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSQ10275.1"
                     /translation="MRIETDDIGMANAAQVAAQGRAAIEGGDARFDLSGVVRCDSSAV
                     AVLLEWERVAVARGLTLEVVAPPAGLVSLATVYGVDGLLPALAPRA"
     gene            complement(3648..4283)
                     /locus_tag="VLN25_06650"
     CDS             complement(3648..4283)
                     /locus_tag="VLN25_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSQ10276.1"
                     /translation="MLLLRTLRFLATLSSVLLVGPALAQPAQTAPDALVRDLTNEVLG
                     AIRADKAIASGDIGRVQKLVDEKVLPYVDFKKMTQLAVGRGWRSATPEQRTALTREFH
                     TLLVRTYSGALSQVKDHQVQMRPFRAQPADTDVLVRTSVVPSRGDPIQLDYRLEKQDA
                     GWKIYDVNVLGVWLVENYKTQFASEISAGGVDGLIKALTDRNRQLAEAKKA"
     gene            complement(4360..5235)
                     /locus_tag="VLN25_06655"
     CDS             complement(4360..5235)
                     /locus_tag="VLN25_06655"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HSQ10277.1"
                     /translation="MTRLLERVLRMWLAAVVIGLAGGCATLPPNAGQDPRDPWEKVNR
                     NVFVFNEGFDANFLKPVAELYAKLPDGLRDCLSNAFFNLRGPSTAINNTLQGKPAEAV
                     SDVGRFVVNTTIGLVGCFDVATELGLEKHYEDFGQTLGVWGFGPGPYLVVPFLGPSNV
                     RDAVGILGVETLLDLNFWIDKPALEYSIFALRIVNLRAELLPTDRLINEAALDPYSFI
                     RDGFLQRRRNLVYDGNPPREIDPEEDDALVPDAQPTKPPGTASSPAGQVKPESAATGS
                     APASEAGVKPQEPGK"
     gene            complement(5270..>5671)
                     /gene="mlaD"
                     /locus_tag="VLN25_06660"
     CDS             complement(5270..>5671)
                     /gene="mlaD"
                     /locus_tag="VLN25_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HSQ10278.1"
                     /translation="ALKAANLASFNTGPTYALTAKFDNIGGLKVRAPVKSAGVTVGRV
                     SAIALDSQSFQGLVTMELDGKVKFPADTSAKILTSGLLGDQYVGLEPGGADKNLAGGD
                     QIKMTQSAVVLENLIGQFLYNRAAEGGTEKK"
BASE COUNT         1031 a         1887 c         1808 g          945 t
ORIGIN      
        1 tgcagttcct gaatgtgcat gaagcggttc tcgaagatcg tctcggtaat gcggccgaca
       61 ccgtcggcaa tgcaatcaag cgccatgaac tgagcctgca tgtcggtcgg aaactcggga
      121 tacggtgccg tgcgcaggct gaccgcccgc ggccgttcgg tcatcctcac ccggatcgcg
      181 tcggccgcgc actcgaggat ggcgccggct tcgcgcagct tgtcgagcac ggcatcaagc
      241 gtcgctgggg ccgcgccacg cagcagcacg tcaccgcccg ccgccgcgac ggcgcacagg
      301 aatgtgccgg tctcgatgcg gtcgggcatc acgcggtgct gcgctccgtg cagacggtcg
      361 acaccctgca cgacgatgcg gttcgtgccg gcaccgtgga tgcgcgcacc catcgcgatc
      421 agcgcattcg ccaggtcacc gacctcgggc tcgcgtgcgg cgttttcgat cacggtttca
      481 ccgttggcga gcgtggccgc catcatcagg ttctcggtgc cggtgacggt caccatgtcg
      541 gtgacgatgc gggtgcccgt cagccgtttc gcctcggcga tcaggtagcc gccctcgatg
      601 tggatgctcg cgcccatcag ctcgagtccc ttgatgtgct gatcgaccgg acgggcaccg
      661 atcgcgcaac cgccgggcag gctcacccgt gcacgcccga accgcgccac cagcggcccc
      721 agcaccagga tcgccgctcg catcgtcttc accagctcgt acggtgcctc gggatgcgtg
      781 accgccgcgg cgccgaggcg cacggtgctg ccctcgcgct cagccacgac tcccatctgc
      841 cgcagcagct tcagcgccgt tgagacatcc tgcaactggg gcacgttgtc gacgatcagg
      901 tcgtcggcgg tcagcaggct ggcgcacagg atcggcagtg ccgcgttctt cgcgcccgaa
      961 atcggaatct cgcccgccag cgacacgccg ccgcggatct tcagcttgtc catgggagcg
     1021 tcggaaattc ctggcagtgt gcggaagacg accgggcgcg taatgcgcac tggcctgcga
     1081 atggcagctt cggcagactc ggcaaacgat tcgagcatgg cagttctcct gcgtcggaat
     1141 ccgcagcgtg cggacgggac tgcagcgtgg ctgcggcgct tgaagcggcg acgaactgcc
     1201 ggtgaaaggc gggtgaagtg tgcacggcca gattcatcct gctgtcgccg cgtattcggc
     1261 cggcgtcagc gtgcgcatcg acaaggcgtg aacttcctcc cgcatccgtt cgcccagggc
     1321 cgcgtacacc agctggtgcc gggccacgcg caacttgcct tcgaaggcag gactgacgat
     1381 cagcgcattg aagtgccggc cgtcaccgtc gacctcgagc acgctgcatt cgagcccggc
     1441 cgcgatgtag cgccgcacgt cgtcgggagt cggatccatc agttcatccc tcactgcgac
     1501 cttcaaggct cactcaatgc ctcagcttgt agccgcgctc cagcaggcgc aaagcgatcg
     1561 cggccgtaag cacaagcgca ccggccacca cggccaggct cacccatggc gagacgtcgg
     1621 actgcccgaa gaagccgtag cggaagccgt cgatcatgta gaaaaacgga ttgaggtgcg
     1681 acacgcgctg ccagaacgca ggcagtccat gcaccgagta gaacacgccc gacagaaatg
     1741 tggcgggcat cacgacgaag ttctggaagg cggcgagttg gtcgaacttg tcggcccaga
     1801 tacccgcgat cacaccgagc gagccgagca tcgcgctgcc ggccagcgca aagaccagga
     1861 tccacagcgg cgccttgaag ctcggcggtc cgaaccagac ggcgaccacg aacacaccca
     1921 ggccgaccac cagcccgcgg acgatcgagg caagcacgta ggcgccaaag agctgccaat
     1981 gggaaaacgg cggcagcagc acgaacacca ggctgcccat gatcttcgaa tggatcaggc
     2041 tcgacgacga gttggcaaag gcgttctgca gcaccgacat catgacgagg cctggaatca
     2101 ggaaggccac atagctgatg ccggggaaga cctcgacgtg atcagccagc acctggccga
     2161 acaccagcag gtacagcacc gccgtcagca ccggcgcggc aatcgtctgg aagctgactt
     2221 tccagaatcg cagcatttcc ttcgtgagca acgtgcgaaa ggcgatcgca gatgacgaat
     2281 ccgccgcgct gatcgtggcg ccttcgcgaa gagtcgccgg cgcgttcatg ccggtgttcc
     2341 cgccgccatc cgttgcgcgg gcttgtccga catcatctgc aggaatacat cctcgaggtc
     2401 cgcgcgtccg acttcaaggt cctcgacgat gcatccggcc gccgcacagt cagccagcac
     2461 ctggccgacg tcgaccgaat gcgcgagtcg caacgtcaac acgccgtcgc gttcatcgat
     2521 ccgatgcgcg agcaccgagg ccggcggtgt cccgcgggga cgcatcgtca gctgcagccc
     2581 tgcaaaacgg gccagcaacg cgcgcgtggt atccagcgcc acgatccggc cgcccttgag
     2641 catagcgata cgcccgcaca gcgcctgcgc ctcctccagg tagtgcgttg tcagcacgat
     2701 ggtgtggcct tcacggttga ggcgccggat gaaatcccac aacgtctgcc gcaactccac
     2761 atcgaccccg gctgtcggct cgtcgagcac gatcaccggc ggcttgtgca ccagcgcctg
     2821 tgcgaccagc agacgccgtt tcatcccgcc cgacagcgcc cgcgtattgg cgtcggcttt
     2881 tgtcgccagg ccgaggttct cgaggacttc atcgatccag gcgtcgttgc cccgcaggcc
     2941 aaagtagcct gactggaagg ccagtgtctc gcgcacggtg aaaaatgggt cgaacgcgag
     3001 ttcctgcggc accacgccga ccgagagccg cgcggcgcgg aagtcgtcga cgacatcgtg
     3061 gcccatgatc gcgacacgac cgctgctggc gcgcaccagg ccggcaatga tcgagatcag
     3121 cgtcgtcttg cctgcgccgt tgggcccgag cagtccgaag aactcgccct cgttgatcac
     3181 caggtcgacg tgatccagcg ccacgagcga gccgtagcgt ttggagacgg aattgacctg
     3241 gatggcagca gtcacgggcg agagtcgacg tgcccggcgg cctcgacaag agacgctcag
     3301 tttatcgaag tcgtcggcag gaagccgacc cgcggccccg gccgcggatc accgtggaag
     3361 cgacgactca ggcgcgtggc gcgagtgccg gcaacaggcc gtcgacgccg tacacggtgg
     3421 caaggctcac cagcccagcg ggcggggcca cgacctcgag cgtcaggccc cgggcaacgg
     3481 ccacgcgctc ccattccagc agcaccgcga ccgcggagga atcgcagcgc acgacgccgg
     3541 acaggtcgaa tcgggcgtcg ccaccctcga tggcagcacg accctgcgcc gccacctggg
     3601 ccgcattggc catgccgata tcgtcggttt cgatccgcat gacgccgtca ggcctttttc
     3661 gcctcggcaa gctggcgatt gcgatccgtc agtgccttga tcagcccgtc gacacctcca
     3721 gcgctgatct cgctggcgaa ctgggtcttg tagttctcaa ccagccagac gccgagcacg
     3781 tttacatcgt agattttcca gccggcatcc tgtttttcca ggcgatagtc gagctggatc
     3841 gggtccccgc gcgacgggac gacactggtc cgcaccagca cgtcggtgtc ggccggctgg
     3901 gcccggaacg gtctcatctg gacctggtga tccttgacct gtgacagtgc gccactgtag
     3961 gtgcggacga gcagcgtatg gaattcgcgc gtcaaggcgg tgcgctgctc gggcgtcgcg
     4021 ctgcgccagc cgcgtccgac ggccagttgc gtcattttct tgaagtcgac atacggcaga
     4081 accttctcgt caaccagctt ctgcacccgg ccgatatcac cgctggcaat ggccttgtcg
     4141 gcacggatgg ccccgagcac ttcgttcgtc aggtcgcgca ccagtgcgtc cggcgcggtc
     4201 tgcgcgggtt gcgccagcgc gggaccgaca agcagcaccg acgacagggt ggcaagaaag
     4261 cgcagggttc gaagcagcag catggttcgt gactcgagag tgaatgtgtc tgacctggag
     4321 actgaccgtg gcgagcgccg atggttcggc cccggaccct tattttcccg gttcctgggg
     4381 cttaacgccc gcctcgctcg ccggtgcact gcccgtggcc gcggactcgg gcttcacttg
     4441 ccctgccggc gaagacgccg tgccgggggg ctttgtcggc tgcgcatccg gcacgagcgc
     4501 gtcgtcttct tcaggatcaa tttcacgcgg cggattgcca tcgtagacaa ggttgcggcg
     4561 acgctgcaga aagccgtcgc ggatgaagga ataggggtcc agagcggcct cgttgatcag
     4621 ccgatcggtc ggcagcagct cggcccgcag gttgacgatg cgcaaggcaa agatgctgta
     4681 ttcgagggcc ggtttatcga tccagaagtt caggtcgagg agagtttcga cgccgaggat
     4741 gccgacggcg tcgcgcacat tgctcggtcc aaggaacggc acgaccaggt agggcccggg
     4801 gccgaagccc cacacgccga gcgtctggcc gaagtcctcg taatgcttct cgagcccgag
     4861 ttcggtcgcc acgtcgaagc agccgacgag gccgatcgtg gtgttcacga cgaatcggcc
     4921 tacatccgag accgcctcgg ccggcttgcc ctgcagcgtg ttgttgatcg ccgtcgacgg
     4981 gccgcgcagg ttgaaaaagg cattggacag gcagtcgcgt aacccatccg gcagctttgc
     5041 gtaaagctcg gccaccggct tcagaaaatt cgcatcgaag ccttcgttga acacgaagac
     5101 attgcgattg accttttccc agggatcgcg cgggtcctgc ccagcgttcg gtggcagcgt
     5161 cgcgcagccg cccgcgaggc cgatgacaac agcggcaagc cacatgcgga gcacccgttc
     5221 aagcagtctt gtcatggctc tgtcgtcggt cggcggaagt catgcatagc tacttctttt
     5281 cggtaccgcc ctcggcggcc cggttgtaaa ggaactgacc gatcaggttc tcgagcacca
     5341 cagcggactg cgtcatcttg atctggtcgc caccggcaag attcttgtcc gcaccgcccg
     5401 gctcaaggcc gacgtactgg tcgcccagca ggccggacgt caggatcttc gccgaggtat
     5461 cggcgggaaa cttgaccttg ccgtccaact ccatggtcac cagcccctgg aagctctgcg
     5521 aatcgagggc aatggcgctc actcggccca cggtcacgcc ggcgctcttg accggtgccc
     5581 tgaccttgag tccgccgatg ttgtcaaact tggctgtcaa cgcatacgtc ggcccggtgt
     5641 tgaagctcgc gaggttcgcg gccttcagcg c
//
LOCUS       DATIRT010000292         1537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1421368, whole genome shotgun sequence.
ACCESSION   DATIRT010000292 DATIRT010000000
VERSION     DATIRT010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421368"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1537
                     /locus_tag="VLN25_06665"
     CDS             <1..>1537
                     /locus_tag="VLN25_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HSQ10279.1"
                     /translation="GFAEGLEEILVVEEKRQFVEYQLKEELFSWIGTGKRIPRVIGKF
                     DEKDGGEWAVPQGNWILPAHYEFSPAIVSKAIVARIGKLELPADIRARMQARLAIIEA
                     KEKALARPRVVTERKPWFCSGCPHNTSTNLPEGSRAVAGIGCHYMVVWMDHNTSTYTQ
                     MGGEGVPWIGQAPFTNEKHIFANLGDGTYFHSGSLAVRAAVAANVSVTYKILFNDAVA
                     MTGGQPTDGQITAPQITQQMHAEGVKKIVIVTDEPSKYDATSELDHSAVNAHGATGDA
                     WNLAPGVTVHHRDELDRIQRELREYPGVSVLIYDQTCASEKRLRRKKNTPEKTAFPDP
                     ARRVVINDLVCEGCGDCSVKSNCLSVEPLETEFGRKRAINQSSCNKDYSCLNGFCPSF
                     VTVEGGKLKKGKSSVTGALPELPEPALPPLRSRGAYGIVVTGVGGTGVVTIGQLLGMA
                     AHLEGRGVSVLDMAGLAQKGGAVFSHVQITPTPEDLFATRIAMGEADVLIGCDLIVTT
                     SNEA"
BASE COUNT          296 a          491 c          512 g          238 t
ORIGIN      
        1 gggctttgcc gaagggctgg aggaaatcct cgtggtcgag gaaaagcgcc agttcgtcga
       61 gtaccagctg aaggaggagc tcttctcgtg gatcggcacc ggcaagcgca ttccgcgcgt
      121 gatcggcaag ttcgacgaaa aggacggcgg cgagtgggcc gtgccgcagg gtaactggat
      181 ccttccggcg cactatgagt tctcgccagc gatcgtctcg aaggcgatcg tcgctcgtat
      241 cggcaagctc gagcttccgg ccgacatccg cgcacgcatg caggcgcggc tggcgatcat
      301 cgaggcgaag gaaaaagcgc tcgccaggcc gcgtgtcgtc accgagcgca agccgtggtt
      361 ctgctcgggc tgcccgcaca acacatcgac caatctgccg gaaggctcac gagcggtggc
      421 cggtatcggc tgccactaca tggttgtctg gatggaccac aacacgtcca cctacacgca
      481 gatgggcggc gaaggcgtgc cctggatcgg ccaggcgccc ttcacgaacg agaagcacat
      541 ctttgccaac ctcggtgacg gcacctactt ccactcgggc tcgctcgcgg tgcgcgcggc
      601 cgtcgcggcc aatgtgtcgg tcacctacaa aattcttttc aacgacgcgg tcgcgatgac
      661 gggcggccag cccacggacg ggcagatcac cgcgccgcag atcacgcagc agatgcatgc
      721 ggaaggcgtg aagaagatcg tcatcgtcac cgacgagccg agcaagtacg acgccaccag
      781 cgaactcgat cacagcgccg tcaacgcgca cggtgccacc ggcgatgcat ggaatctggc
      841 cccgggcgtt accgtgcatc accgcgacga gctcgaccgc atccagcgcg aactgcgcga
      901 atacccgggc gtatcggtgc tgatctacga ccagacctgc gcctcggaaa agcgccttcg
      961 gcgcaagaag aacacgccgg agaaaacggc gtttccggac ccggcgcgcc gcgtcgtgat
     1021 caacgacctc gtctgcgaag gctgcggcga ctgcagcgtc aagtccaatt gcctctcggt
     1081 cgagccgctg gagaccgagt tcggccgcaa gcgcgcgatc aatcagtcga gctgcaacaa
     1141 ggactactcc tgcctgaacg ggttctgccc gagtttcgtc acggtggaag gcggcaagct
     1201 gaaaaagggc aagtcgtcgg tcacgggcgc gttgccggag ctgccggagc cggcgctgcc
     1261 gccgttacgg tcgcgcggcg cgtacggcat cgtggtgacc ggcgtcggtg gaaccggcgt
     1321 cgtgaccatc ggccagctgc tcggcatggc cgcacatctc gagggacgcg gcgtgtcggt
     1381 gctggacatg gccggactgg cgcagaaggg cggcgcggtg ttctcgcatg tgcagatcac
     1441 gccgacgccc gaggatctct tcgcgacgcg catcgcgatg ggcgaggccg atgtcctgat
     1501 cggctgcgac ctgatcgtca ccaccagcaa cgaggcg
//
LOCUS       DATIRT010000293         3658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1224848, whole genome shotgun sequence.
ACCESSION   DATIRT010000293 DATIRT010000000
VERSION     DATIRT010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224848"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VLN25_06670"
     CDS             complement(<1..642)
                     /locus_tag="VLN25_06670"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HSQ10280.1"
                     /translation="MQVRTLPAAAGWAWLREGLALYRRQAFAFTALVIMYSMALMLIA
                     SVPVIGLPLAAVLVPFGTLGITAAGRNAERNIMPLPSLLIDGFKGAQRNALLRLGAVH
                     AGLIVLLVAVTSFFARDELASWQVVEGQVDPASIAGNVPWDAIGVATLVYTPILMLTW
                     FAPQLVAWHRYTVGKALFFSLFACWRNRWPFLVLAIALGALIVAVSWVASMLMN"
     gene            complement(667..1647)
                     /locus_tag="VLN25_06675"
     CDS             complement(667..1647)
                     /locus_tag="VLN25_06675"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HSQ10281.1"
                     /translation="MAVFTALTRAEAASFLTQFSIGELTELQGIASGIENTNFFLTTT
                     QSRYVLTIFERLTFKQLPFYLELMQHLSRRGLPVPAPQENRSGALLSEIKGKPAAIVT
                     RLEGKAVVQPTPRHCALVGGVLARMHRAGADFPMFQPHLRGIGWWKDSVPRLEPHIPD
                     DVFHRLADELIYQDSFFRSARFERLPAGPIHADLFRDNVLIVSTPSGDAIGGLIDFYF
                     AGCSIWLFDLAVTMNDWCIDLATGAFDPARAQAMIDAYHAARPLTKEEHECWRTVLRA
                     AALRFWISRLYDFYLPRPAEVLMPHDPTHFERILARRIADATLPWVAAAA"
     gene            complement(1665..2168)
                     /locus_tag="VLN25_06680"
     CDS             complement(1665..2168)
                     /locus_tag="VLN25_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10282.1"
                     /translation="MALAIGFGMPAIVLAQAAPLAPLDPRASPATPNAGMPTSKPAAP
                     RTRIELPPAERQALESGKSENRPTTPSRNELLPNADDASTTIIDEPANVIEQIRTSNR
                     ITEIRVTPAMTGHTYIMTNREGRQPTTATDTTSGLSVPKFFTFEFGRQADRPATTLPP
                     PPSSTSR"
     gene            complement(2316..3008)
                     /locus_tag="VLN25_06685"
     CDS             complement(2316..3008)
                     /locus_tag="VLN25_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HSQ10283.1"
                     /translation="MPERATSQKARESWQLWGIISEFVEATERLSEIRPAVSIFGSAR
                     SLPGSPHYELTVEIARKLSDAGFAVISGGGPGIMEAANKGAFEGASPSVGLNIELPFE
                     QSGNAYQDISLRFRHFFARKVAFVKYAAAYVVMPGGFGTLDELSEALTLMQTGKGKKM
                     PVVLVGSAFWGGLLQWMRSTLAVEGMISESDLEMMRVVDDSDAVVNALFDFYDARGFE
                     QSATEREQLLYL"
     gene            3066..>3658
                     /locus_tag="VLN25_06690"
     CDS             3066..>3658
                     /locus_tag="VLN25_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HSQ10284.1"
                     /translation="MKTLLLLDGSSYLYRAFHALPDLRTAAGEPTGALRGFISMLRTL
                     RQQVAADYLACVFDAKGKTFRDDLYSEYKANRAAMPDDLVAQIEPIHAAVRALGWPVI
                     EIEGIEADDVIGTLARYAGEHGLRTVISTGDKDLAQLVDEHTTLVNTMNGETLDPAGV
                     EAKFGVPPVRIVDYLTLVGDSVDNVPGVDKVGPKTAA"
BASE COUNT          675 a         1133 c         1220 g          630 t
ORIGIN      
        1 gttcataagc atcgatgcaa cccaggatac ggcgacgatc aacgctccga gtgcgatcgc
       61 cagaacaaga aacggccagc gattgcgcca gcacgcaaac agcgagaaga acagcgcctt
      121 gccgaccgtg taacggtgcc aggccacgag ctgcggcgcg aaccaggtca gcatcaggat
      181 cggcgtgtac acgagcgtgg ccaccccgat ggcgtcccag ggaacattgc ccgcaatcga
      241 ggccgggtcc acctgcccct cgacgacctg ccagctggcc agctcgtcgc gggcgaagaa
      301 cgacgttacc gctaccagca gtacgatcaa tccggcatgg acagcaccga ggcgcagcag
      361 cgcgttgcgc tgtgccccct tgaagccgtc gatcagcagc gatggcagcg gcatgatatt
      421 tcgctcggcg ttgcgcccgg cggccgtgat gcccagcgtg ccgaacggca ccagcacggc
      481 agccagcggc aggccgatga ccggcacgct ggcgatcagc atcagcgcca tcgagtacat
      541 gatgacgagt gcggtgaacg cgaacgcctg gcgccggtac agggccagtc cttcgcgcaa
      601 ccaggcccat ccggcggccg ccggcagcgt tctgacttgc atggtccgga ttctatcggc
      661 gcgtcgtcac gcggcggccg caacccacgg caacgtggca tcagcgatgc ggcgcgcgag
      721 gatgcgttcg aagtgggtcg gatcgtgcgg catcaggact tcggccgggc gcgggaggta
      781 gaagtcgtac aggcgcgaga tccagaagcg cagcgcagct gcacgcagga cggtgcgcca
      841 gcattcatgc tcttctttgg tcagcggtcg cgcggcgtga taggcgtcga tcattgcctg
      901 cgcgcgcgcc gggtcaaacg cgccggtcgc caggtcgatg caccagtcgt tcattgtgac
      961 cgcgaggtcg aaaagccaga tcgagcagcc ggcaaagtag aagtcgatca gtccgccgat
     1021 ggcatcgccg gacggcgtag acacgatcag cacgttgtcg cgaaagaggt ccgcgtggat
     1081 cgggccggcg ggtaatcgct cgaagcgggc gctccggaag aacgaatcct ggtagatgag
     1141 ttcgtcggcc agtcgatgga aaacatcgtc ggggatgtgc ggctcgagcc tgggcactga
     1201 gtccttccac catccgatgc cgcgcagatg tggctggaac attggaaagt ccgcgccagc
     1261 cctgtgcatg cgcgcgagta caccgcccac cagtgcacag tgccgcggcg tcggctgaac
     1321 gacggccttt ccttcgagcc gggtcacgat cgcagccggt tttcccttga tctccgagag
     1381 cagcgcacca ctgcgatttt cctgcggcgc cgggaccggg agtccgcggc gggagagatg
     1441 ctgcatcagt tcgaggtaga acggaagctg cttgaacgtg aggcgttcga agatcgtcag
     1501 cacgtagcgc gactgcgtcg tcgtgaggaa aaagttggtg ttctcgatgc ccgaggcaat
     1561 accctgcagc tcggtcagtt cgccgatgga aaactgcgtc aggaagctcg cggcttcggc
     1621 gcgagtaagc gcggtgaaaa cggccatcgg gtcgggccgg tgaactagcg cgaagtggag
     1681 gaagggggag gggggagcgt tgtggcgggg cgatccgcct gccgaccgaa ttcaaacgtg
     1741 aagaacttcg gcacggacaa gccggatgtc gtgtcagtcg ccgtggtcgg ctggcggccc
     1801 tcgcggttgg tcatgatgta ggtgtggccg gtcatggcgg gggtcacacg gatttcggtg
     1861 atgcgattgg aggtcctgat ctgctcgatc acgttcgcgg gctcgtcgat gatcgtggtg
     1921 ctggcgtcgt ccgcgttggg tagcaattcg ttccggctag gcgtcgtcgg gcggttctcg
     1981 gacttgccgc tttcgagcgc ttgccgttcc gcagggggga gctcaatgcg ggtccgcgga
     2041 gccgctggct tgctcgtcgg catgccggcg ttcggagtag ccggcgacgc ccggggatcc
     2101 aacggcgcga ggggtgccgc ctgcgcgaga acaatggcag gcataccaaa gccgatcgcc
     2161 agcgccattg ccacaggtgt cacgcgggca acgaggcccc cgcgggtcgg tagcctgcgc
     2221 ttgatcgggc gctcgggatt cgtcggtcgc attggagcaa ttctaaccgg gctgccgcca
     2281 tccaccggtc cactcatgtc aatcattgtg cgcactcaca gatagagcag ctgttcgcgc
     2341 tcggtcgcgc tctgctcgaa tccgcgtgca tcgtagaagt cgaagagcgc attgacgacg
     2401 gcatcgctgt cgtcgaccac acgcatcatc tcgaggtccg actcactgat catgccttcg
     2461 actgccagtg tgctgcgcat ccactgcagc aagccgcccc agaatgccga acccaccagt
     2521 accaccggca tcttcttgcc cttgcccgtc tgcatcagcg tcagggcttc gctcagttcg
     2581 tcgagcgtgc cgaacccgcc cggcatgacg acataggcgg ccgcgtactt gacgaaggcg
     2641 accttgcgcg cgaaaaaatg gcgaaagcgc aacgagatgt cctggtacgc gttgcccgat
     2701 tgctcgaacg gtagttcgat gttcagtccg accgacgggg acgctccttc gaaggcaccc
     2761 ttgttggccg cttccatgat gcccgggccg cctccgctga tcacggcgaa tcccgcgtcc
     2821 gacagcttgc gcgcgatctc gaccgtcagc tcgtagtgcg gggaccccgg caggctgcgc
     2881 gcgctgccga agatcgagac ggcagggcga atctccgaca gccgctcggt cgcctcgacg
     2941 aactctgaaa taatgcccca cagctgccat gactcccgtg ccttctggct ggtggcgcgc
     3001 tcgggcatcg tgactgcgcg caggctgggt atgttcttgc ggtggtcaag cacgttcact
     3061 ctccaatgaa aaccctcttg ttgctcgacg gatcgagcta tctgtaccgc gccttccatg
     3121 cgctacccga cctgcgtacg gcggcgggcg agcccacggg ggcgctgcgc ggcttcatct
     3181 caatgttgcg aacgctacgc cagcaggtcg cggccgacta cctggcgtgt gtcttcgatg
     3241 ccaagggtaa gacgtttcgg gacgaccttt actccgaata caaggccaat cgtgccgcca
     3301 tgccggacga tctggtagca cagatcgagc cgatccatgc cgcggtgcgg gcgctgggct
     3361 ggccggtgat cgaaatcgag ggcatcgaag ccgatgatgt gatcggcacg ctggctcgct
     3421 acgccggcga gcatggcctg cgcaccgtaa tttcgaccgg cgacaaggat ctcgctcagc
     3481 tggtggacga gcacacgacg ctggtgaaca cgatgaacgg ggagacgttg gatccggccg
     3541 gcgtcgaagc caagttcggc gtgccacccg tgcgcatcgt cgactacctc acgctggtcg
     3601 gtgacagcgt ggacaacgtg cccggcgtgg acaaggtcgg acccaagact gccgcaaa
//
LOCUS       DATIRT010000294         5404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_339850, whole genome shotgun sequence.
ACCESSION   DATIRT010000294 DATIRT010000000
VERSION     DATIRT010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339850"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1809
                     /gene="alaS"
                     /locus_tag="VLN25_06695"
     CDS             <1..1809
                     /gene="alaS"
                     /locus_tag="VLN25_06695"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011884423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HSQ10285.1"
                     /translation="LKVIADHIRACAFLIADGVIPGNEGRGYVLRRIIRRGLRHGYKL
                     GQTRPFFHRIVKDLVAEMGAAYPELKEQEARIEAVLKQEEERFGETLENGMKILDAAL
                     GQMKSGDARMLDGETAFLLYDTYGFPVDLTGDICRERGVSLDHAGFNDAMERQREQAR
                     AAGKFRMAAGVEYAGDKTRFTGYASLADDGKVVALYHEGSAVPRLEKGQSGIVVLNST
                     PFYAESGGQVGDRGVLEAISDGHVVRFTVPDTQKIQADVFGHHGTLESGTLSVGDVVQ
                     ARVDAAIRERTRNNHSATHLMHSALRAVLGKHVQQKGSLVDADRTRFDFSHDKAVTPD
                     EIRRIEDLVNTAIRANAQVTANVMKYDDAIKAGALAFFGDKYGDEVRVLAMGDHSTEL
                     CGGTHVARTGDIGLFKIVAEGGVAAGVRRVEAVTGKGALEVVQALDRELHEAAHLLKA
                     PASELTQKIAQVQDHVKALEKELARLKSKIASNQGDDLAAQAIDIKGVKVLAAALDGA
                     DAKTLRETLDQLKSKLKSAIVVLGAVEAGKVQLAAGVTPDAVGKVKAGDLVNYVAQQV
                     GGKGGGRADMAMAGGTDPAGLPAALASVKSWAEQRL"
     gene            1788..2336
                     /locus_tag="VLN25_06700"
     CDS             1788..2336
                     /locus_tag="VLN25_06700"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSQ10286.1"
                     /translation="MGGTASIDGVVIRRAEPADATGIARTFQSRSAAAGTLQNPYPSV
                     ADWVERLGRDAASNYIFVALSGDDVIGHSGLHSNKNPRRQHAWGLGISIREDWQRRGV
                     GTRLMETVIDLADNWLGALRLELTVFSDNAAALALYRKFGFEVEGVHRAYALRVGQYC
                     DTTAMARLHPKPPQLHPAGTSS"
     gene            2373..2903
                     /locus_tag="VLN25_06705"
     CDS             2373..2903
                     /locus_tag="VLN25_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HSQ10287.1"
                     /translation="MSAAYRFCPQCATPLMPRTVAERERGSCTACGWVHWDNPTPVVA
                     ALIEYKGRILLARNRAWPEKMFALVTGFLERDETPRQAIEREVAEETSLATRTATLIG
                     VYEFMRKNEVIIAYHVEADGEICLGEELVDHRLIEPARLRPWPLGTGLAMADWMRGRG
                     LPVEFLSFPPASASNA"
     gene            2912..3151
                     /locus_tag="VLN25_06710"
     CDS             2912..3151
                     /locus_tag="VLN25_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HSQ10288.1"
                     /translation="MDTSPTADKEIDTRGLNCPLPILRAKKALADMTSGQVLKVLATD
                     PGSVRDFQAFAKQTGNELVGQQTVAKEYMHYLKRR"
     gene            3148..3816
                     /locus_tag="VLN25_06715"
     CDS             3148..3816
                     /locus_tag="VLN25_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10289.1"
                     /translation="MSTPTPGQAPTHAVRSSPSRLKLVFWLLLVPVALVAGYFAIVLN
                     WNYSSGERAGWVQKFSRKGWLCKTWEGEMAMVTMPGAIPEKFPFTVWDDNVAAQINRV
                     MGKRVTLHYEQKVGLPTTCFGETRHFVSGVVAVDEIPLGPGLVVPLPGQGPVVTPPVS
                     TPPAMTPPAAPPPSSPAPAPTAPAPVTSPPSEYTMPAPAEPVRPPAAPGSPPTTPDSP
                     PAAK"
     gene            complement(3862..>5404)
                     /locus_tag="VLN25_06720"
     CDS             complement(3862..>5404)
                     /locus_tag="VLN25_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HSQ10290.1"
                     /translation="EHGNVYVARSEVEAQAKAGAGKRLSRDPDVLDTWYSSAMVPFST
                     LGWPDVEQQATGAERVAYDLYLPSTVLVTGYDIIFFWVARMVMMTTHFTGRVPFRDVY
                     IHGIVRDAEGKKMSKSEGNTLDPIDIIDGIDLEALVKKNTMGLRKTEDAPKVAAKVRK
                     HFPEGIAPYGADALRFTMAAYATLGRNINFDLKRCEGYRNFCNKLWNATRFVLMNTEG
                     KDCGRDEKLPVELSAVDRWIIGELQRTEVAVADGFALYRLDNVANSVYSFVWNEYCDW
                     YLELAKVQLQFGNAEQQRGTRRTLVRVLEVALRLAHPVIPFISEELWQAVSVLAGKRT
                     ADTESSVMIQPFPRAQPEKVDASADRQMALLKRMVDALRNLRSEMNLSPAQKVPLLAS
                     GDSATLASFSLYLQALARLSDVQVVVDVNAAAKGAAAPIKVVDEYRLMLKIEVDVAAE
                     RDRLGKEIARLENEIRKAEVKLGNAGFVERAPAAVVAQEKERLAGFGATLVKVREQFE
                     RLPAA"
BASE COUNT          955 a         1725 c         1808 g          916 t
ORIGIN      
        1 ctgaaggtga tcgccgatca catccgcgcc tgcgcgttcc tgattgccga cggcgtgatt
       61 ccgggcaatg aagggcgtgg ctatgtgctg cgtcggatca tccggcgtgg gctgcggcat
      121 ggctacaagc tgggacagac ccggccattt ttccacagga ttgtgaagga tctggttgcc
      181 gagatgggcg cggcctatcc ggagctgaag gagcaggagg cgcgcatcga ggcggtactt
      241 aagcaggagg aggagcgctt cggcgagacg ctcgaaaacg gcatgaagat tctcgacgcc
      301 gcgctcggcc agatgaagtc gggcgatgcc cgcatgctcg acggcgagac cgcatttctc
      361 ctatacgaca cctacggctt cccggtcgac ctgaccgggg acatttgtcg cgagcgcggt
      421 gtgtcgctgg atcacgccgg cttcaatgac gcgatggagc gccagcgcga acaggcgcgc
      481 gcagcgggca aattccgaat ggcggccggc gtcgagtacg cgggggacaa gacgcgcttc
      541 accggctatg cgtcactcgc tgacgacggc aaggtggtgg ccctgtatca cgagggcagc
      601 gcggtgccgc ggctcgaaaa gggacagagc ggcatcgtgg tgctcaattc gacaccgttc
      661 tacgcggagt cgggaggcca ggtcggcgac cggggcgtgc tcgaggccat cagtgacggc
      721 cacgtcgtgc gcttcactgt gcccgacacg cagaagattc aggccgatgt cttcggccac
      781 cacggcacgc tggagtctgg caccttgtcc gtcggcgatg tcgtgcaggc gcgcgtcgac
      841 gcagccatac gcgaacgcac gcgcaacaac cattcggcca cgcacctgat gcactcggcg
      901 ctgcgggctg tgctcggcaa gcacgtgcaa cagaagggct cgctggtcga cgccgatcgc
      961 actcgtttcg atttttcgca tgacaaggcg gtcacgccgg acgagatccg tcgcatcgaa
     1021 gacttggtca atacggcgat ccgtgccaat gcgcaggtga ctgccaacgt gatgaagtac
     1081 gacgacgcga tcaaggccgg cgcgctggcc ttcttcggcg acaagtacgg tgacgaagtg
     1141 cgcgtgctgg caatgggtga tcactccacg gagctgtgcg gcggcacgca cgtcgcgcgt
     1201 acgggggaca ttggtctatt caagatcgtt gccgaaggcg gcgtggcggc cggcgtacgg
     1261 cgcgtcgagg cagtcacagg gaagggagcg ctggaggtgg ttcaggcgct cgatcgtgaa
     1321 ctgcacgagg ctgctcacct gctgaaggcg cccgcatcgg aactgacgca gaagatcgcg
     1381 caggtgcagg accacgtgaa ggcgctggaa aaggagcttg cgcgcctcaa gagcaagatc
     1441 gcgtcgaacc agggcgacga tctcgccgcg caggcgatcg acatcaaagg cgtcaaggtg
     1501 ctggcggctg cactggacgg cgcggatgcg aagaccctgc gtgagacctt ggatcagttg
     1561 aagtcgaagc tgaagagcgc gatcgtcgtg ctcggcgcgg tcgaggccgg caaggttcag
     1621 ctcgcggccg gtgtgacgcc cgacgcggtt ggtaaggtga aggcgggcga cctcgtgaac
     1681 tatgtggcgc agcaggtagg aggcaagggc ggaggccggg ccgacatggc catggccggt
     1741 ggcaccgatc cagcggggct tccggccgcg ctcgccagcg tgaaatcgtg ggcggaacag
     1801 cgtctataga cggtgttgtc atccggcgcg cggagcccgc ggatgccacg ggcatcgcac
     1861 gcacgttcca gtcgcgcagc gccgcggcgg gcacgctgca gaacccgtat ccgtcggtgg
     1921 ccgactgggt cgagcggctg ggtcgtgacg ccgcgagcaa ctacatcttc gtcgcactgt
     1981 caggtgatga cgtcatcggt cactcgggcc tgcacagcaa caagaacccg cgtcggcagc
     2041 acgcctgggg gctcggcatc agcatccgcg aggactggca gcggcgggga gtcggcacgc
     2101 gcctgatgga gaccgtcatc gatctggccg acaactggct cggtgcactg cgtctcgagc
     2161 tgacggtgtt cagcgacaac gcggccgcgc tggcgctgta tcgcaaattc ggattcgagg
     2221 tcgaaggcgt gcatcgcgcg tatgccctgc gtgtaggcca gtactgcgac accacggcga
     2281 tggcgagact gcatccgaag cccccgcagc tacacccggc cgggacttct tcttgagcgt
     2341 cgcgcgtcgc gcgtcgcggg gcggcgactg aaatgtccgc cgcctatcgc ttctgccccc
     2401 agtgcgccac gcccctgatg cctcgcacgg tggccgagcg cgagcgcggc agctgcaccg
     2461 cgtgcggctg ggtgcactgg gacaatccga cccccgttgt cgcggcactg atcgaataca
     2521 agggccggat cctgctggcg cgcaaccggg cctggcccga gaagatgttc gcgctggtca
     2581 cgggcttcct cgaacgtgac gagacaccgc ggcaggccat cgagcgcgag gtggcggagg
     2641 agacttcgct cgcgacgcga acggcgacgt tgatcggcgt ctacgagttc atgcgcaaga
     2701 acgaggtcat catcgcctac catgtggagg ctgacggcga gatctgtctt ggtgaagaac
     2761 tcgttgacca tcgactgatc gagcccgcgc ggctgcggcc ctggccactg ggtacggggc
     2821 tcgccatggc cgactggatg cgcgggcgcg gtctgccggt agaatttctt tctttcccac
     2881 cggcgtctgc ttcaaacgcc tgaaacgtcc aatggacacc tccccgacag ccgacaagga
     2941 aatcgatacg cgcggactca actgcccgct tccgatcctg cgtgcaaaga aggcactggc
     3001 ggacatgacg agcggccagg tcctgaaggt ccttgcgact gatcccggat cggtgcgcga
     3061 tttccaggca tttgcgaagc agaccggcaa cgagctggtc ggccagcaga ccgtggcgaa
     3121 ggaatacatg cactacttga agcgccgatg agcactccga ctcccggcca ggcaccgaca
     3181 cacgccgtgc ggtcgtcacc gtcgcgcctg aagctcgtct tctggttact gctcgtacca
     3241 gtggcgctgg tcgcggggta cttcgcgatc gtgctcaact ggaactactc cagcggcgaa
     3301 cgcgccggct gggtgcagaa gttcagcagg aagggctggc tctgcaagac ctgggaaggc
     3361 gagatggcga tggtcacgat gccgggcgcg ataccggaga agtttccgtt caccgtctgg
     3421 gacgacaacg tagcggcgca gatcaatcga gtgatgggca agcgcgtgac gctgcactac
     3481 gagcagaaag tcggcctgcc gaccacctgc ttcggcgaga cgcggcactt cgtctcaggc
     3541 gtcgtggctg ttgatgaaat tccgctggga cctggcctcg tggtgccgct gccgggacag
     3601 gggcccgtcg taacaccgcc ggtctccacg ccgcctgcca tgacgccgcc ggcagcaccg
     3661 cctccatcct ctccagcgcc cgcgccgacc gctcccgcgc ctgtgacatc tccgccttct
     3721 gagtacacga tgcccgcgcc ggctgaaccg gttcggcctc ccgccgcacc gggctcaccg
     3781 ccaaccacgc cggactcacc gcccgccgcg aaataaccgc gcggaccacg gccctgcccg
     3841 acgccggtca ctcgccggcc gtcaggccgc cggcaggcgc tcaaactgct cgcgcacctt
     3901 gacgagcgta gcgccgaagc cggccagtcg ctccttctcc tgtgcgacga cggctgccgg
     3961 tgcgcgctcg acgaagccgg cgttgccgag cttgacctcg gccttgcgga tctcgttttc
     4021 gagccgcgca atctccttgc cgagccgatc acgctctgcc gcgacatcga cctcgatctt
     4081 cagcatcaat cggtattcgt cgacgacttt gatcggcgcg gccgccccct tcgctgccgc
     4141 gttgacgtca acgacgacct gcacgtcgga cagccgcgca agggcctgca ggtacaggga
     4201 aaacgacgcg agcgtcgcgc tgtcgcctga tgccagcagt ggaaccttct gcgccggcga
     4261 gagattcatc tcgctgcgca ggttgcgcag cgcatcgacc atacgcttga gcagcgccat
     4321 ctggcgatcg gccgaagcgt cgaccttttc gggctgtgca cgcgggaacg gctggatcat
     4381 caccgacgac tccgtatcgg cggtgcgctt gccggccagc acagagacag cctgccacag
     4441 ttcttcggag atgaacggaa tgactggatg cgccaggcgc aacgcgactt ccagcacgcg
     4501 taccagcgtt cgacgcgtgc cgcgctgctg ctccgcgttg ccgaattgca gctgcacctt
     4561 ggccagttcg aggtaccagt cgcagtactc gttccagacg aagctgtaga cgctgttggc
     4621 aacgttgtcg agccggtaca gtgcaaatcc gtcggcgacg gccacttccg tgcgctgcag
     4681 ttcgccgatg atccagcgat cgaccgccga aagttccacc ggcagcttct cgtcgcggcc
     4741 gcagtccttg ccctcggtgt tcatcagcac gaagcgcgtg gcattccaca gcttgttgca
     4801 gaagttgcgg taaccctcgc agcgcttgag gtcgaagttg atattgcggc cgagcgtggc
     4861 gtaggccgcc atcgtgaagc gcagcgcgtc ggcgccgtac ggcgcgatgc cctcgggaaa
     4921 gtgcttgcgc accttggccg cgaccttggg tgcgtcctcg gtcttgcgca accccatcgt
     4981 gttcttcttg accagcgcct cgaggtcgat gccgtcgatg atgtcgatgg gatcgagcgt
     5041 gttgccttcg ctcttcgaca tcttcttgcc ctcggcatcg cgcacgatgc cgtggatgta
     5101 gacgtcgcga aacggcacgc ggccggtgaa gtgggtcgtc atcatcacca tgcgtgcgac
     5161 ccagaagaag atgatgtcgt agccggtgac gagcaccgtc gacggcaggt acaggtcgta
     5221 ggcgacgcgc tccgcacccg tcgcctgctg ttcaacgtcc ggccagccca gtgtggaaaa
     5281 cggcaccatc gccgacgagt accaggtgtc gagtacgtca gggtcgcgcg acagccgctt
     5341 gcctgctcct gccttggcct gcgcttcgac ctcggagcgc gcgacgtaga cgttgccgtg
     5401 ctcg
//
LOCUS       DATIRT010000295         2866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1912626, whole genome shotgun sequence.
ACCESSION   DATIRT010000295 DATIRT010000000
VERSION     DATIRT010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1912626"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..1288)
                     /locus_tag="VLN25_06725"
     CDS             complement(182..1288)
                     /locus_tag="VLN25_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSQ10291.1"
                     /translation="MGAVLRKEFIQLRRDRLTFAMLIGVPIMQLVLFGYAINSDPRNL
                     PTAVVALDQSPFVRSIVRAAQNTNYFHIIEVGSESEAERLIARGDVQFALVFPSDFSA
                     RLVRGESPAMTVYADATDPAATGPAVSALQRLPQLALRNDLHGPLARLAPGREPFEVI
                     VHRRYNPEGLTNYNVVPGLMGVILTMTLVMMTAMGMTRERERGTLENLLATPVRPVEV
                     MIGKIVPYVIIGYAQVLIVFTAARWLFEVPMFGNFFLLSSAIVLFIIATLGVGFAFST
                     IARSQMQSMQMTMFFFLPNILLSGFMFPFRGMPTWAQWIGEALPLTHFLRIVRGVMLK
                     GAGLADVAPQTVPILIFIAVAAAVAMKRYKQTLD"
     gene            complement(1386..2312)
                     /locus_tag="VLN25_06730"
     CDS             complement(1386..2312)
                     /locus_tag="VLN25_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSQ10292.1"
                     /translation="MSPDLAIDVRGLTKIFSGRTVVDHIDLAVPRGQVCGFLGPNGSG
                     KTTTIRMLCGLLEVDAGEGTCLGYDIRRESRRIKLRVGYMTQRFGLYEDLSIAENLDF
                     TARVYRLDNRRQRVADALDRLGLTTRKAQLAGTLSGGWKQRLALAACLLHEPELLLLD
                     EPTAGVDPKARRDFWREIHALAADGITVLVSTHYMDEAERCHRLVYISYGKMLAAGTA
                     PEVIAQAGLRTWAVHGPRLDAVLDAVERRGWMAVPFGTSLHVSSTDHDDFPRWLAEHD
                     PALAREMRIEPIPTGLEDVFIALTQDARDTVS"
     gene            complement(2323..>2866)
                     /locus_tag="VLN25_06735"
     CDS             complement(2323..>2866)
                     /locus_tag="VLN25_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HSQ10293.1"
                     /translation="EARAARERDAARVAEIQAQLATARDAARPDEIAAAEADQRAAES
                     DLASMQWREDQTLGRSTATATVHDVMYRPGEWVGQGLPVIALLPDGAVKVRFFVPQAA
                     LTRIRVGDSVAVSCDGCPPGLAARVAFVSNQAEYTPPVIYSNESRSKLVFMVEAKPDE
                     KSAQILKPGQPLDVRIAAGS"
BASE COUNT          490 a          951 c          961 g          464 t
ORIGIN      
        1 ctccgtcgat gttgcggctg ctgtcagcag caaggccatc aaggtggcgc acgcagttcg
       61 tcgatccatg cgtcgatcca tgccggcagc gtagcgcgtc gccgggccat ggaagggaca
      121 cgcactcgct tccagaccta cgattgccga ttgatttgcc gccgcgggac gctgctgccc
      181 gtcagtcgag tgtctgtttg tagcgcttca tcgcgacggc ggcggcgacg gcgatgaaga
      241 tcaggatcgg caccgtttgc ggtgcgacgt cggcgagccc cgcgcccttg agcatcacgc
      301 cgcgcacgat gcgcaggaaa tgcgtcagcg gcagcgcctc gccgatccat tgcgcccacg
      361 tgggcatgcc gcggaacgga aacatgaatc cggacagcag gatgttcggc aggaagaaga
      421 acatcgtcat ctgcatcgac tgcatctgcg aacgggcgat cgtcgagaac gcgaagccga
      481 caccgagcgt ggcgatgatg aacagcacga tggccgagga cagcaggaag aagttgccga
      541 acatcggcac ctcgaaaagc caacgcgctg ccgtgaagac gatcagcacc tgtgcatagc
      601 cgatgatcac gtacggcacg atcttgccga tcatcacctc gaccggacgc accggggtgg
      661 cgagaaggtt ctccagcgtg ccacgctcgc gctcgcgcgt catgcccatc gccgtcatca
      721 tcaccagcgt catcgtcagg atcacaccca tcaggcccgg aacgacgttg taattggtga
      781 gtccttcggg gttgtagcgg cggtgcacga tcacctcgaa cggctcgcga cccggagcca
      841 gccgcgccag gggaccgtgc aggtcgttgc gcagcgcgag ttgcggcaat cgttgcagcg
      901 ccgataccgc cggcccggtg gctgcgggat cggtagcgtc cgcgtagacg gtcatcgccg
      961 gcgactcgcc acgaacaagc cgcgcgctga agtccgatgg aaacacgagt gcgaactgga
     1021 catcgccgcg cgcgatcagg cgctcggcct cgctctccga gccgacttcg atgatgtgaa
     1081 agtagttcgt gttctgtgcc gcgcgcacga tgctgcgtac gaacggcgac tggtcgagcg
     1141 ccacgaccgc cgtcgggagg ttccggggat ccgagttgat cgcgtagccg aacagcacga
     1201 gctgcatgat cggcacgccg ataagcatcg cgaacgtcag gcggtcgcgc cgcaactgga
     1261 tgaactcctt gcgcagcacg gcaccgatgc gctcgagcga gagcgcccag cgccacggcc
     1321 gatcgtggga gcctccgcgc tgcgcgctgc ggcgcgagct cgcttggggc ggcccggcgc
     1381 ttcgctcagc tgactgtgtc gcgggcatcc tgcgtcagtg caatgaagac atcctcgagc
     1441 ccggtcggta tcggctcgat ccgcatctcg cgcgcgagcg ccgggtcgtg ctccgccagc
     1501 cagcgaggaa agtcgtcgtg gtcggtgctg ctgacatgca aactggtgcc gaacggcacc
     1561 gccatccagc cgcgccgttc aaccgcatcg agcacggcgt cgagccgggg gccgtgcacg
     1621 gcccaggtcc tgagcccggc ctgtgcaatc acttcggggg ccgtgccggc cgccagcatc
     1681 ttgccgtagg agatgtagac gaggcgatga cagcgctcgg cctcgtccat gtaatgggtg
     1741 ctgaccagca ccgtgatgcc gtccgcggcg agcgcgtgga tctcgcgcca gaagtcgcgg
     1801 cgcgctttcg ggtcgacacc ggccgtcggc tcgtcgagca gcagcagctc cggctcgtgc
     1861 aacaggcagg ccgcgagagc gaggcgctgc ttccagccgc ccgacagcgt gccggccagc
     1921 tgtgccttgc gcgtcgtcag tcccagacga tcgagcgcat cggcgacgcg ctggcgacgg
     1981 ttatccagac ggtagacacg cgcggtgaag tcgaggtttt cggcgatcga caggtcttcg
     2041 tacaggccga accgctgcgt catgtagccg acccgcagct tgatgcggcg cgattcgcgg
     2101 cggatgtcgt agccgaggca ggtgccttcg cctgcatcga cctcgagcag tccacacagc
     2161 atgcggatcg tcgttgtctt gccgctgccg ttcgggccaa ggaacccgca gacctgcccg
     2221 cgcggcaccg cgaggtcgat gtggtcgacc acggtgcggc cgctgaagat cttggtcagt
     2281 ccgcggacgt cgatcgccag atccgggctc atcagggcgg gcctagctgc ctgcggcgat
     2341 gcggacgtcg agcggctggc cgggtttgag aatctgcgcc gacttttcgt ccggctttgc
     2401 ctcgaccatg aacacaagct tggatcggct ctcgttgctg tagatgaccg gcggcgtgta
     2461 ctcggcctgg ttggacacga acgcaacgcg tgccgcgagc cccgggggac agccgtcgca
     2521 actcacagcc acgctgtcgc cgacacggat acgcgtcagc gccgcctgcg gcacgaagaa
     2581 ccgcaccttg actgcgccat caggcagcag agcaatcacc ggcaaaccct gtccgaccca
     2641 ctcgccgggc cgatacatga cgtcgtgcac ggtcgccgtc gcggtggacc ggccgagcgt
     2701 ctggtcctcg cgccattgca ttgacgccag atcgctctcg gcagcgcgct ggtcggcctc
     2761 ggccgccgcg atctcgtcgg gccgcgctgc atcgcgggcg gttgccagct gcgcctggat
     2821 ctcggcgacg cgtgcagcat cccgttcgcg agctgcacgc gcttca
//
LOCUS       DATIRT010000296         7702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1717448, whole genome shotgun sequence.
ACCESSION   DATIRT010000296 DATIRT010000000
VERSION     DATIRT010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7702
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1717448"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..763)
                     /gene="ahpC"
                     /locus_tag="VLN25_06740"
                     /pseudo
     CDS             complement(200..763)
                     /gene="ahpC"
                     /locus_tag="VLN25_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017114658.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
     gene            940..1833
                     /locus_tag="VLN25_06745"
     CDS             940..1833
                     /locus_tag="VLN25_06745"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HSQ10294.1"
                     /translation="MSASRPFDSSHWLAWFFVTVWGSGYLATKVGLQYAPPFTFLTLR
                     FVFGIALVLPIALVARPNWPRGALLGHVIVAGLLMHAVNLGGSHYAQYLGMSAGIAAL
                     ILALQPLVTAMVAAPLLGERPRPPQWLGVALGLFGVALVVWHKIDVRALTPPALFAVS
                     IALAAITAGTLYQRIFCPAVDLRAAGAVQFIASALVLAPLAVTVEGFPVDWSWPLVAS
                     ILFLVVLASILAVNALHTLMRRGQATRVTSLLYLTPIIAVALEWDMFDVRPTLLTAVG
                     IAVTCAGVALVAWRPAVPSTA"
     gene            1830..3011
                     /locus_tag="VLN25_06750"
     CDS             1830..3011
                     /locus_tag="VLN25_06750"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSQ10295.1"
                     /translation="MTLSLGRIIGFVVLTHTAFGAARVTLSLYALSNKASTFTVGVLM
                     ALFALVQALLAVRAGRWLDRVGPFRPLLLGTALMTGGALLPAAFPYAVADVAPLLVAS
                     TLLGTGFMYLQMTAQNLVGVLADPTRRPAAFSVLALGFSTSGLIAPVSSGFMIDAFGH
                     RATFAAIFVLMATSLLLLLTQRRRLPSPDVHGRESEASDPFELLRHTEVRAVLIVSGV
                     ISMAWDLQSFMIPVYGTSIGLTASQIGLVLGSFAAATFAIRLAMPVLSRRYREWQVLL
                     FTLAASAIAFALMPLFSSMAPLMAVAFLLGLGLGSAQPNVMSLLHERAPTGRVGEALG
                     LRTTIMNSSHVVLPLVIGAFGSVLGAAAGFWLMATALVGGGWSAWGRMKLESPRPGRT
                     Q"
     gene            3008..3649
                     /locus_tag="VLN25_06755"
     CDS             3008..3649
                     /locus_tag="VLN25_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HSQ10296.1"
                     /translation="MSADDGSPRRLSYEELEASLEAFLREWNGSTPLWVFGYGSLIWK
                     PELSFDMRRPARVHGYHRRLCLWSRINRGTPQCPGLVAGLDRGGSCTGVVYRVPATAV
                     RSQFSQLWQREMMTNSYAPRHLECRLDDGSLVSAVAFVVRRDASNYAGRLNERQILDV
                     FARGSYGRYGTSLEYLVNTICALRSHGIVDPHLERLARHAGVDAATWRLGDAA"
     gene            3660..4295
                     /gene="pdxH"
                     /locus_tag="VLN25_06760"
     CDS             3660..4295
                     /gene="pdxH"
                     /locus_tag="VLN25_06760"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HSQ10297.1"
                     /translation="MDEIARLRTDYMKGALDDEHADPHPIRQFTRWWAEATKSLLREA
                     NAMTLATADRSGVPSARMVLLKGYDDRGFVFFTNYDRRKGRELADNPRASLLFFWSEL
                     ERQVRIEGAVERIAAGESDEYFRSRPLTSRIGAWASPQSEPIPSKAWLLARAAEMGLR
                     HGLNPARPPQWGGYRVRPEVMEFWQGRPSRLHDRLRYTRSGDAWQRARLAP"
     gene            4409..5080
                     /locus_tag="VLN25_06765"
     CDS             4409..5080
                     /locus_tag="VLN25_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSQ10298.1"
                     /translation="MSEQPITIIIVEDEPEFRRRFVQIVESEPSMKLVGVAANKREAQ
                     VIVDKEVFDVMLIDLGLPDGSGIELIRQVSQKKPDVDIMVVTVFGDEQHVVSSIEAGA
                     TGYILKDSAPADVISCIRLLRAGGSPVSPVVARSVLRAIRNRMGTTAPARVQGAEQNP
                     LSARETEILQLLAKGMSFNEIGEILGISPHTVTAHIKKIYRKLAVHSRGEAVYEATQM
                     GLLRS"
     gene            5077..7053
                     /locus_tag="VLN25_06770"
     CDS             5077..7053
                     /locus_tag="VLN25_06770"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSQ10299.1"
                     /translation="MSLRSPADVPRGGRIALNLASVCGAGRVIRTLVLLLSLLAPLTP
                     AVAEEATRLTDIEFLADQRTTVPGPDAVWQTHTLPMRWSSSSGARQGLWLRLSFDLPE
                     VPTHGWGLLLQRLPTGGAIYLNGRRVADLPTDSDAQRVRWRRPHLINLPTEFLRAGRN
                     EALIFTSYGNGVHGVGAVEVGSTAALQPRYGREFFASHTLRWISLLLTVLLAVGFAAL
                     WFRRRSESLFGLLSLIAIFWALRSLDSTFEVLSNDAYQLSRWAFYLGTAVFTALATIT
                     LWRQSGRPHGRAEVGIAVLASMGPVLYMLSGDSFDLTVGPLWLAIMLGALTLGVLSLA
                     RRAFSEESLMLRFIVAALLIALAAALHDFLVATGALPFASAALLNIATPVLVIALGAA
                     LIDRFVRSLADVEKTNTELETRIHEREQLLKRNFDRLRESERVKASAQERQRIMQDMH
                     DGLGSQLLSSLMLVERGAMTNEQVAQILRESIDDMRLAIDALAAEDSDLLAALGNMRF
                     RMEPRLKVAGMELQWDARNLPEEVDIDPNGVLPVLRIVQEALTNAIKHSRARVVRVTL
                     GVDHDGDAQWLSIRITDNGRGLAATSNVTGRGMLNMKSRAGRIAAFLKVESVPGAGTM
                     ILLRVPLDLSPGPTTRAMQLSLDTEAVIEQLRKE"
     gene            7074..>7702
                     /locus_tag="VLN25_06775"
     CDS             7074..>7702
                     /locus_tag="VLN25_06775"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HSQ10300.1"
                     /translation="MQSRAVPPDSLFDDEISPTTRGVASVRAAEFDDAIVRLGRQLPA
                     SVHLGTSSWSFPGWQSIVYGDAYSESALARVGLTAYAQHPLLRLAGIDRGYYQPLPRS
                     DFVRYASQVPAAFRFLVKAPALVADAFMRGERGAPAEPNPYFLDVGTAIDQFIVPAIE
                     GLASRAGPLLFQLPPLPREMSVGDATHSTIDRLGAFLGALPRRVGEIAPL"
BASE COUNT         1144 a         2573 c         2559 g         1426 t
ORIGIN      
        1 cgtcgagcag ttcgcgcatc tcgcgcgagg cttcgcgctc gtcgagcgac gcgacgatct
       61 cgaccgtgtc cgtgagacgc tgcagatacg cagcaagctg ggtcttgagg gtgtcgtcca
      121 acatggctaa ctccgaggtg agacaaagtg acgcccccgc cggcgtgccg ccggcggagg
      181 ctattacaga cgggatcgat cagatcttgc cgacgaggtc gagcgacggg gtcagcgtct
      241 tggcgccctc ctgccacttg gccggtcaga cctgacccgg gttgttggcg acgtactgcg
      301 cagcttgcag cttgcgcagc gtctccttga cgtcacgcgc gatcgcgttg tcgtggattt
      361 cggcggtctt gaccacgccg tccggattga cgatgaacgt gccgcgcagc gccaggccct
      421 cttcctcgat gtgcacgccg aacatgcgag tcagcgtgtg cgtcgggtcg ccgaccagcg
      481 ggaacttcgc cttgccgaca gccgggctgg tctcgtgcca caccttgtgc gcgaaatgcg
      541 tgtcggtcgt gacgatgtag acctcggcgc cgagcgtctt gaacaggtga tagttgtcgg
      601 ccgcgtcctc gacttccgtc gggcagttga aggtgaaagc cgccggcatg aacaccagca
      661 ccgaccactt gcccttgagg gactggtcgg tcacttcgat gaacttgccg ttatgaaacg
      721 cctgggcctt gaagggctgg accgcggtgt tgatgatcga catggtgttt gtccttgatt
      781 ggatgggtag gggaatcaaa tctgaggact gaatactgag gctatcgatg ccgctattcc
      841 aattctattt ccctaattgg ccgatagtct gtctcctcgg gacggcgaaa tggcggccgc
      901 cagcgcagcg gcggcttcgg cgtctgcaag aatccggtcg tgagcgcctc ccgaccattc
      961 gactccagtc actggctggc ctggttcttc gtcaccgtct ggggttcggg atacctggcg
     1021 accaaggtcg gattgcagta cgcgcctccg ttcacgttcc tgacgctgcg cttcgtcttc
     1081 ggcatcgcac tggtgttgcc gatagcgctg gtcgcgcgtc ccaactggcc gcgcggcgca
     1141 ctgctggggc acgtcatcgt cgccgggctg ctgatgcacg cggtcaatct cggcggcagc
     1201 cactatgcgc agtacctcgg catgtcggcc ggcattgcag cgctgatcct ggcgctgcag
     1261 ccgctcgtca ccgcgatggt cgcggcgccg ctgctcggcg agcgtccccg gcccccgcaa
     1321 tggctcggcg ttgcgctcgg tctgttcggc gtcgcgttgg tcgtctggca caagatcgac
     1381 gtacgggcgc tgacgccgcc tgcactcttc gcggtatcga tcgcgctcgc agcgatcacc
     1441 gccggcacgc tctatcagcg catcttctgt ccagccgtcg acctacgcgc ggctggcgcc
     1501 gtgcagttca tcgccagcgc gctggtgctg gctccccttg cggtcaccgt cgagggcttc
     1561 ccggtcgact ggtcgtggcc tctcgtagcc agcatcctgt tcctggtcgt gctcgcctcg
     1621 atcctcgcgg tcaacgcgct gcacacgctg atgcgtcgcg gccaggcgac gcgcgtcacc
     1681 agcctgctgt acctgacgcc gatcattgcg gtcgcgctcg agtgggacat gttcgatgtg
     1741 cgcccgacat tgctgaccgc cgtcggcatc gcggtgacct gcgccggagt ggccctggtc
     1801 gcgtggcgcc ccgcggtacc gagcactgca tgacactgag cctgggccgc atcattggct
     1861 tcgtcgtgct gacgcacacg gctttcggcg ccgcgcgcgt cacgctgtcg ctctatgcgc
     1921 tgtcgaacaa ggcctcgacg ttcacggtcg gtgtgctgat ggcgctcttc gcgctggtgc
     1981 aggcactgct ggcggtgcgg gccggccgct ggctcgaccg ggtcgggccc tttcgcccac
     2041 tgctgctcgg cacggccctg atgaccggcg gggcgctgtt gcccgcggca tttccctatg
     2101 ccgtggcgga cgtcgccccc ttgctggtgg cgtctacgct gctcggcacg gggttcatgt
     2161 acctgcagat gacggcacag aacctggtgg gtgtcctggc ggatccgacg cgacgtccgg
     2221 ccgctttttc cgtgctggcg ctggggtttt cgacatctgg cctgatcgct ccggtctcga
     2281 gcggattcat gatcgacgcc ttcggccatc gggcgacgtt tgccgcgatc ttcgtgctga
     2341 tggccaccag cctcctgctg ttgctgaccc agcgccggcg actgccatcg cccgatgtgc
     2401 acggcaggga gtcggaagcg tccgacccgt tcgagctgct gcgacatacc gaagtgcggg
     2461 cggtgctgat cgtcagcggc gtgatctcca tggcctggga cctgcagtcg ttcatgatcc
     2521 ccgtctacgg caccagcatc ggcctgacgg cgtcgcagat cggcctggtg ctcggcagtt
     2581 tcgccgcggc gaccttcgcg atccggctgg ccatgcccgt gctgtcgcgt cgctatcgtg
     2641 aatggcaggt gcttctgttc acactggctg cttcggccat cgcctttgct ctgatgccgc
     2701 tcttcagttc gatggccccg ttgatggcag ttgcgttcct actcgggctc ggcctgggtt
     2761 cggcgcaacc caacgtgatg tcgctcttgc acgaacgtgc accgacgggg cgcgtcggag
     2821 aagcgctcgg cctgcgcacg acgatcatga acagcagtca cgtggtcctg ccgctggtga
     2881 tcggcgcgtt cggctctgtg ctcggcgcgg ccgccggctt ctggctgatg gcgacggcgc
     2941 tggtcggcgg cggctggagc gcatggggcc ggatgaagct cgaatcgccg cggccaggac
     3001 ggacacaatg agcgcggatg acggatcgcc gcgacgcctc agctacgagg aactcgaagc
     3061 ctcgctggag gcgttcctgc gggagtggaa tggatcgacc ccgctctggg tcttcggcta
     3121 cgggtccctg atctggaagc cggaactttc gttcgacatg cgtcgtccgg cacgcgtgca
     3181 cggctaccat cgccggctct gtctgtggag ccgcatcaac cgcggcacgc cacagtgccc
     3241 ggggctcgtg gccgggctcg accgcggagg ctcatgcacc ggcgtcgtgt accgggtacc
     3301 ggcaaccgcg gtgcgcagcc agttctcgca gctctggcag cgcgagatga tgaccaattc
     3361 gtatgcgccg cggcacctcg agtgccggct cgatgacggg tcgctcgtgt ccgcggtcgc
     3421 gtttgtcgtg cggcgtgacg cgtccaacta cgccggccgg ctcaatgaac gccagatcct
     3481 ggacgtattc gcgcgcggca gctatggacg ctacggcacg agtctcgaat acctcgtcaa
     3541 tacgatctgc gcgctgcgat cgcacggcat cgtcgatccg cacctggagc ggctcgcgcg
     3601 ccatgccggc gtcgacgccg cgacctggcg tctcggcgac gctgcctaaa atggcgccca
     3661 tggacgagat tgcacgcctc cgcaccgact acatgaaggg cgcgctcgac gacgagcacg
     3721 ccgatccgca tccaatccgc cagttcacgc gctggtgggc cgaggcgacg aagtcgttgt
     3781 tgcgggaggc gaacgcaatg acgctcgcca ctgccgaccg cagcggcgtc ccgtcggcgc
     3841 gcatggtgct gctcaagggc tatgacgacc gcggcttcgt ctttttcacc aattacgaca
     3901 gacgcaaggg ccgcgagctg gccgacaatc cgcgcgcgtc attgctgttc ttctggtccg
     3961 aactggagcg ccaggtgcgg atcgaaggcg cggtcgaacg gatcgcggca ggtgagtcgg
     4021 acgagtattt tcgcagccgc ccgctgacga gccgcatcgg cgcctgggcc tcaccgcaga
     4081 gcgagccgat ccccagcaag gcctggctgc tggcgcgcgc cgccgaaatg ggactgcgcc
     4141 atggcctgaa tccggcgcgg ccgccgcagt ggggcggcta ccgggtgcgg cctgaggtga
     4201 tggaattctg gcaggggcgg ccctcgcgac tgcacgatcg gctgaggtat acgcgcagcg
     4261 gcgacgcctg gcagcgtgcg cgcttggcac cctagccgca tcccgcgacg cccgtcccga
     4321 gattggaccg gattcgcacc agcgctcggg ccatgtcccg gcggtgtcca gaggctatga
     4381 tcgcgcattc gcagggttgg agggttggat gagcgaacag ccgataacga tcatcatcgt
     4441 cgaggacgaa ccggaattcc ggcgtcgctt cgtgcagatc gtcgaaagtg agcccagcat
     4501 gaagctggtc ggcgttgctg ccaacaagcg cgaggcgcag gtcatcgtcg acaaggaagt
     4561 cttcgacgtc atgctgatcg acctcggcct gcccgacggc agcggcatcg agctgatccg
     4621 ccaggtcagc cagaagaagc ctgacgtcga catcatggtc gtgaccgtgt tcggcgacga
     4681 acagcatgtc gtttcgtcga tcgaggcagg cgcgaccggc tacatcctga aagactcggc
     4741 cccggccgac gtcatctcgt gcatccgcct gctgcgcgcc ggcggttcgc cggtgagtcc
     4801 ggtggttgca cgcagcgtgc tgcgcgcgat ccgcaatcgc atgggcacga ccgccccggc
     4861 acgcgtgcag ggcgcggagc agaacccgct gtctgcgcgc gaaaccgaga tcctgcaact
     4921 gctcgccaag ggcatgagct tcaacgagat cggagaaatc ctcggcatct cgccacatac
     4981 cgtcaccgcg catatcaaga agatctatcg caagctggcc gtgcactcgc gcggcgaggc
     5041 ggtgtacgag gccacgcaga tgggtctgct gcgcagttga gtctccgctc gcccgccgac
     5101 gtcccacgcg gcggccgtat cgccctgaac ctcgcatccg tctgcggcgc gggccgcgtc
     5161 atccgcacgc tggtcctgct gctgagtttg ctcgcgccgc tcacgccagc cgttgcggag
     5221 gaagcaaccc gacttaccga catcgagttc ctcgccgacc agcgcacgac cgttcccggc
     5281 ccggacgccg tctggcagac gcacacactg ccgatgcgtt ggtcgtcgtc gagcggggcg
     5341 cgccagggcc tgtggctgcg actgagcttc gacctgcccg aggtgcccac ccacggctgg
     5401 gggctgctgc tgcagcgact gccgacgggc ggcgccatct acctgaatgg ccgtcgggtg
     5461 gcggacctgc ccaccgactc cgatgcacag cgcgtccgct ggcgtcggcc gcacctgatc
     5521 aacctgccga cggaattcct ccgcgccggt cgcaatgaag cgctgatctt cacttcctat
     5581 ggcaacggcg tgcacggagt cggcgccgtt gaggtcgggt cgacggccgc gctgcagccg
     5641 cgctatgggc gcgagttttt cgccagccac acgctgcgct ggatttcgtt gttgctgacc
     5701 gtgctgctcg cggtgggatt tgccgcgctc tggttccgac gtcgcagcga gagcctgttc
     5761 ggactcctgt cgctgatcgc gatcttctgg gcgctgcgtt cgctcgattc gaccttcgaa
     5821 gtcctgtcga acgacgcgta tcagctgagc cgctgggcgt tctaccttgg cacagcggta
     5881 ttcaccgcac ttgcgaccat caccctgtgg cgccagagcg gacggccgca cggccgcgcc
     5941 gaagtcggaa tcgcggttct cgcatccatg ggtccggtgc tctacatgct gtcgggcgac
     6001 tcgttcgacc tgacggtcgg accgctgtgg ctggcgatca tgctgggcgc gctgacgctc
     6061 ggagtgctgt ccctggcacg ccgggcattc agcgaggaat cgttgatgct tcgcttcatc
     6121 gtcgctgcgc tgttgatcgc gttggcagcc gcgctgcacg atttcctcgt cgccaccgga
     6181 gcgctgccct tcgccagcgc cgcgctgctg aatatcgcca cgccggtgct cgtgatcgcc
     6241 ctcggcgccg cgctgatcga tcgcttcgtg cgctcgctcg ccgacgtcga gaaaaccaac
     6301 accgagctgg aaacgcgcat ccacgagcgc gaacagctgc tcaagcgcaa tttcgaccgc
     6361 ctgcgcgaaa gcgaacgcgt caaggccagc gcgcaggagc gccagcgcat catgcaggac
     6421 atgcacgatg gcctcggctc gcaactgctg tcgtcgctga tgctggtcga gcgcggcgcc
     6481 atgaccaatg agcaggtggc gcagatcctg cgcgaatcga tcgacgacat gcggctggcg
     6541 atcgatgcgc tggccgccga ggactccgac ctgctggcgg cgctgggcaa catgcgcttc
     6601 cgcatggagc cgcgcctcaa ggtggccggc atggaactcc aatgggacgc ccgcaacctg
     6661 ccggaggaag tcgacatcga tccgaacggc gtgctgcctg tgttgcgcat cgtgcaggaa
     6721 gcattgacca acgccatcaa gcacagccgt gcgcgggtcg tgcgcgtcac gctcggtgtc
     6781 gaccacgacg gcgatgcgca gtggctttcg atccgcatca ccgacaacgg ccgcgggctg
     6841 gctgcgacca gcaatgtcac cgggcgcggc atgctgaaca tgaagagccg cgccggtcgg
     6901 attgcggctt tcctgaaggt cgagtccgta cccggcgcgg gcaccatgat cctgctgcgt
     6961 gtgccgctcg acctgagccc gggcccgacg acacgggcga tgcagctttc tctcgatacc
     7021 gaggcggtga tcgaacagct ccgcaaggag tagcccgcct tagactgcac cccatgcagt
     7081 cgcgagccgt ccccccggat tcactgttcg acgacgagat ctcgccgacc acgcgcgggg
     7141 ttgcgagcgt tcgggcggcg gagttcgacg acgccatcgt gcggctcggg cggcagttgc
     7201 cggcgagcgt gcatctcggt acgtcgtcgt ggagctttcc gggctggcag agcatcgtgt
     7261 acggagatgc gtacagcgag tccgcgcttg cgcgggtggg cctgaccgcc tatgcacagc
     7321 atccgttgct gcgcctggcc ggcatcgatc gcggttacta ccagccgctg ccgcgcagcg
     7381 acttcgtgcg ctacgcaagc caggtgccgg ccgcgttccg gttcctggtt aaggccccgg
     7441 cgctggttgc ggacgcgttc atgcgcggcg agcgcggcgc cccggccgag ccgaatccgt
     7501 actttctcga cgtcggcacg gccatcgacc agttcatcgt gccggccatc gagggcctcg
     7561 cgtcacgtgc ggggccgctg ctcttccagc tgccgccctt gccgcgcgag atgtccgtcg
     7621 gcgacgcaac ccactcgacc atcgaccggc tcggcgcatt ccttggagcg ctgccccgtc
     7681 gcgtcggcga gatcgcaccg ct
//
LOCUS       DATIRT010000297         3430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_734093, whole genome shotgun sequence.
ACCESSION   DATIRT010000297 DATIRT010000000
VERSION     DATIRT010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734093"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VLN25_06780"
     CDS             complement(<1..228)
                     /locus_tag="VLN25_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10301.1"
                     /translation="MNDGIAGWLLALAISAVLIPWSEREQRPAAVGAFALLLLYVVAL
                     AMGGWLLAPHWRPWSPAIAAAAVWLAFQPLLA"
     gene            complement(236..844)
                     /locus_tag="VLN25_06785"
     CDS             complement(236..844)
                     /locus_tag="VLN25_06785"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HSQ10302.1"
                     /translation="MFTGLVQGIGQIERVEPCLGGGGVRITVDGSRVPGFTVQVGDSI
                     ALNGACMTATTVDGMRFTADVSGESLARTVGLDAPGDVNIETSLRLGDAIGGHLVAGH
                     VDGVGEVTRHAAVGESWELVIVAPPELGKYLAVKGSIAVNGVSLTVNRVTDTPAGCEF
                     SLNIIPHTQAVTTLHQLRHGARVNLEADLIARYVERMLPGTN"
     gene            complement(855..1949)
                     /gene="ribD"
                     /locus_tag="VLN25_06790"
     CDS             complement(855..1949)
                     /gene="ribD"
                     /locus_tag="VLN25_06790"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HSQ10303.1"
                     /translation="MYDASDREFMSRALGLAARAMNDAAPNPRVGCVIVREGRVIGQG
                     WTQPPGSNHAEIEALLDARRNGHDVRGATVYVSLEPCSHFGRTPPCSTALIEAHVGRV
                     IAAVEDPNPLVAGTGLEMLRSADIDVRCGLLEQEAAEMNIGFFSRMQRGRPWVRMKIA
                     ASLDGKTALESGASKWITSEEARHDGHAWRARACVLLTGIGTVRADNPQLDVRLVETP
                     RQPLRVLVDSRLEVDPAARLFDGAGKVLVATVSSVPERAAALRARGVELLELPDAQGK
                     VDLVALMRELARRHINEVHVEAGFKLNGSLVKAGVVDELLVYLAPMLIGEAQGMLHLP
                     ALSDLAHAQRLEFLEVAPVGSDVRIRARLV"
     gene            complement(1949..>3430)
                     /locus_tag="VLN25_06795"
     CDS             complement(1949..>3430)
                     /locus_tag="VLN25_06795"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HSQ10304.1"
                     /translation="TSDRDQARQAIDALPNPTAPGDVRLRTEADARLHTAAVTLDALR
                     AQQSALTSLRGMAPLAVELWNQRYTAINSKDPEARRTAIAALTTTFSRVRSLQSYASD
                     LGILIDTAVQEQQRRVDGLDPKSPGRRYEEAALATIRRADETVGELQVAALKVRAAST
                     HWSREFATAAADRTVDEKLAEAWVETKDVARDVWNFELFAVEDTIDIGGRSTTVSRGV
                     TIGKSIGALLIFVVGFSIAGALARRAEHVMVTRFAVADSQAKVLRRWVMMLTGFVLLV
                     LTLNLARIPLTVFAFMGGALAIGIGFGTQTLLKNLISGIIVLFERKVRVGDIVDVEGV
                     QGIVTAVDIRSTTVRQFDGIETMVPNAMLLENKVTNWTGESPTMRRVVKVGVAYGSPT
                     RQVADILQQAAAEHGLVIKDPAPLVIFEDFGDNALIFALYFWVDVAKNSGIQVQSDLR
                     FMLEKRLNDAGISIAFPQRDIHLDTARPLQIEVVAATPSAPAA"
BASE COUNT          589 a         1191 c         1093 g          557 t
ORIGIN      
        1 tgccagcagc ggctgaaatg cgagccacac ggccgccgcc gcgatggcag gcgaccaggg
       61 gcgccagtgc ggcgcaagca gccacccgcc catcgcgagc gcaacgacat aaagcagcag
      121 cagtgcgaaa gcgccgaccg cggccgggcg ctgctcgcgc tcgctccatg gaatcagtac
      181 ggcgctgatc gccagcgcaa gcagccagcc ggcgatcccg tcgttcatgg cggagtcagt
      241 tagtgccggg aagcatgcgc tccacgtagc gcgcaatcag gtcggcctcg aggttgacgc
      301 gcgcaccgtg acgcagctga tgcagtgtcg tcacggcctg ggtgtgcgga atgatgttga
      361 gcgagaactc gcagcccgcg ggtgtatcgg tgacgcggtt cacagtcagg ctcacgccgt
      421 tgacggcgat cgatcccttc acggcgaggt acttgccgag ctcgggtggc gcgacgatca
      481 ccagttccca cgattcgccc accgcggcat ggcgtgtcac ctcgccgaca ccatcgacat
      541 ggcccgcgac gaggtggcca ccgatcgcgt cgcccaaccg caacgaggtc tcgatgttca
      601 catcgccggg tgcgtccaga cccacggtcc gggccaggct ttccccggac acatcggcgg
      661 tgaagcgcat cccgtcgacc gtcgttgccg tcatgcaggc gccgttgagc gcaatgctgt
      721 cgcccacctg aaccgtgaaa ccgggaacgc gcgagccgtc gaccgtgatg cgaaccccgc
      781 cgccaccgag acagggctcc acccgctcaa tctgcccgat gccctgcacg agacccgtga
      841 acatgcgtaa gccctcagac cagacgcgcg cggatgcgca cgtcgctacc cactggcgca
      901 acctcgagaa actcgagccg ctgcgcatgg gccaggtcgg aaagtgccgg caggtgcagc
      961 atcccctgcg cctcaccgat cagcatcggc gccaggtaga ccagcagttc gtcgacgacg
     1021 cccgccttga ccagcgaccc gttcagcttg aaccccgctt cgacatgtac ttcgttgatg
     1081 tgccggcgcg cgagctcacg catcaacgca accaggtcga ccttaccctg tgcgtccggc
     1141 aattcgagca attcgacacc gcgggcacgc agagccgccg cgcgctccgg cacggaactc
     1201 actgtggcga ccagcacctt gccggcaccg tcgaagaggc gcgccgcggg atcgacctcg
     1261 agccgactgt cgaccagcac gcgcagcggc tggcgcggtg tctcaaccag acgcacatcg
     1321 agctgcggat tgtccgcccg caccgtgccg atgcccgtca gaagaacgca ggcccgtgcg
     1381 cgccacgcat gtccatcatg gcgcgcctcc tcggacgtga tccacttcga cgcgccgctt
     1441 tccagggccg tcttgccgtc caggctggcc gcgatcttca tgcggaccca gggtcgcccg
     1501 cgctgcatgc gcgagaaaaa accgatgttc atctcggccg cttcctgttc cagcaggccg
     1561 cagcgcacgt cgatgtctgc actgcgcagc atctcgagtc cggtgccggc caccagcgga
     1621 ttcgggtcct cgacggcggc aatgacgcgg cccacatgcg cctcgatcaa tgccgtggaa
     1681 cagggcggcg tgcggccaaa gtggctgcac ggctcgagcg acacatagac cgtcgcgccg
     1741 cgcacgtcgt ggccgttgcg acgtgcgtcg agcagtgcct cgatctccgc gtggttggat
     1801 cccggcggct gggtccagcc ttggccgatg accctgcctt cgcgcacgat gacgcagccg
     1861 acgcgcggat tgggcgccgc gtcgttcatc gcgcgcgcgg ccagtcccag cgcgcgggac
     1921 atgaattcgc ggtcggatgc gtcgtacatc aggctgccgg cgccgatggc gtggccgcga
     1981 ccacctcgat ctgcagcggc cgcgcggtgt cgaggtggat gtcccgctgc ggaaaggcga
     2041 tcgaaatgcc cgcatcgttc aagcgcttct ccagcatgaa tcgcaggtcg ctctgcacct
     2101 gaatgccgct gttctttgcc acgtcgaccc aaaagtacag cgcgaagata agggcgttgt
     2161 cgccgaagtc ctcgaagatc acgagcggcg ccgggtcctt gatgaccagg ccatgttcgg
     2221 cggcggcctg ctgcaggatg tcggccacct ggcgcgttgg cgatccgtag gcgacgccaa
     2281 ccttcaccac gcgccgcatc gtcggcgatt cgccggtcca gttggtgacc ttgttttcga
     2341 gcagcatggc attcgggacc atggtctcga tgccgtcgaa ctgccgcacg gtcgtcgaac
     2401 ggatgtcgac cgcggtgacg atgccctgga cgccctcgac gtcaacgatg tcgccgaccc
     2461 gcaccttgcg ctcgaacagc acgatgatgc cgctgatcag gttcttcagc agtgtctgtg
     2521 tgccgaagcc gatgccgatg gcaagcgcac cgcccatgaa ggcgaaaacg gtgagcggta
     2581 ttcgcgccag gttgagcgtg agcaccagca gcacgaatcc cgtcagcatc atgacccagc
     2641 gtcgcaggac cttggcctgc gagtccgcta cggcaaagcg ggtcaccatc acgtgctcgg
     2701 cgcggcgcgc caaggctccc gcaatcgaaa agccgacgac aaagatcagc agggcgccaa
     2761 tactctttcc tatcgtcacg ccacgtgaca ccgtcgtcga acgtccaccg atatcgatcg
     2821 tgtcctcgac ggcgaagagt tcgaagttcc agacgtcccg tgcgacgtcc ttcgtttcga
     2881 cccaggcttc ggccagtttt tcatccacag tgcgatcggc cgcagcggtg gcgaactcgc
     2941 gactccagtg cgtcgaggcg gcacgaacct tcagtgcggc gacttgcagt tcgccgaccg
     3001 tctcgtcggc gcgccggatc gtcgccagtg ccgcctcttc atagcgccgc cccggggatt
     3061 tcgggtccag cccgtcgacg cgacgctgct gctcctggac cgccgtatcg atcaggatgc
     3121 ccaggtcgct cgcatacgac tgcagggacc gcacccgact gaacgtggta gtgagcgccg
     3181 caatggccgt ccgccgcgct tccggatcct tgctgttgat cgccgtgtaa cgctggttcc
     3241 agagctcgac agccagcgga gccatgccgc gcagcgacgt cagcgcgctc tgctgtgccc
     3301 gcagcgcatc gagggtgacg gccgcggtat gcagccgtgc gtcggcctcc gtgcgcaggc
     3361 gtacatcgcc gggcgccgtg ggattgggca gcgcgtcgat cgcctggcgg gcctggtctc
     3421 gatcgctggt
//
LOCUS       DATIRT010000298         1554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1422842, whole genome shotgun sequence.
ACCESSION   DATIRT010000298 DATIRT010000000
VERSION     DATIRT010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422842"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VLN25_06800"
     CDS             complement(<1..452)
                     /locus_tag="VLN25_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10305.1"
                     /translation="MKSGLVEEREPNEAALIAEMIVILRRKMARDYAKGGTLRDAHPK
                     TLGLLRGRFRIESNLPKALRVGLFGKPGSFDCWVRFSNSSGKVQSDAVPDARGVAIKL
                     LAPRGKGSAAGAELGQDFVLLDTPVMPLGTVALFRDAVYYTIESSPLLL"
     gene            714..1403
                     /locus_tag="VLN25_06805"
     CDS             714..1403
                     /locus_tag="VLN25_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HSQ10306.1"
                     /translation="MRHSERHRTHRTGWLRAAVLGANDGIVSTASLVLGVAAAGAGTQ
                     GILVAGVAGLVAGSMSMAAGEYVSVSSQADTERADLARETKELAANPEQEHAELTAIY
                     VKRGLDDSLASKVSTQLMQHDALAAHGRDELGISDTLAARPVQAALASAGTFAVGAAL
                     PLLMVLLLPASALMWGLAGSSLLFLALLGLVAARAGGAPVIASVMRVAFWGALAMALT
                     AAVGALFGVAA"
BASE COUNT          264 a          463 c          493 g          334 t
ORIGIN      
        1 agcagcaacg gcgagctttc aatcgtgtag tagacagcat cgcgaaacag cgccactgtg
       61 cccagcggca tcaccggtgt gtccagcagg acgaagtctt gaccgagttc tgcgccggca
      121 gcggaaccct tgccgcgtgg cgccagtaac ttgatcgcga caccgcgcgc atcgggcacc
      181 gcgtcggact ggactttgcc actggaattg gagaagcgca cccaacaatc gaagctaccc
      241 ggcttcccga atagccccac tcgaagcgcc ttgggcaggt tggactcgat ccggaagcgg
      301 ccgcgcaaca ggccgagcgt cttcgggtgc gcgtcgcgta gcgtcccgcc cttggcatag
      361 tcccgcgcca tcttgcgacg caggattacg atcatctcgg cgatcagggc cgcctcgttt
      421 ggttcgcgtt cttcgaccag accggacttc attgtgtgac gctaacagtg aatcaagacg
      481 gcgttgcggt tacagcagtc catcgctctg tgaacgctgc cgtcagctga acctgcgcac
      541 atttcgcgcc ttcctcggca tgttttgact gcactcacgg tttcgtcgac cgtccgatgg
      601 acaatgcaag tgtggacgct tgcgtgacaa ctgtccgtgc gcttgcgaac actgaggagg
      661 gtgcttgcta tgccgtttgt tttagtggcg ggacttgccg gagtggtgac ccaatgagac
      721 acagcgaaag acacagaaca catcgcacag gctggcttcg tgccgccgtt ctcggtgcga
      781 acgacggcat cgtttctacg gccagccttg ttctgggggt cgcggctgca ggcgcaggca
      841 ctcagggcat cctggtcgca ggcgttgccg gcctcgttgc tggttcaatg tcaatggcag
      901 ccggcgagta cgtgtccgtc agctcgcaag cagataccga acgcgcggac ctggctcggg
      961 agaccaagga gttggctgcg aatcccgagc aggaacacgc agaactgacg gcgatttatg
     1021 tcaaacgcgg actggacgac tcactggcat cgaaggtttc cacccagttg atgcagcatg
     1081 acgcacttgc ggcgcatggt cgagatgaac tcggtatatc cgataccctg gctgcacgac
     1141 ccgtgcaggc tgcattggct tcggcgggta cgtttgccgt gggcgcggct ttacctcttc
     1201 tgatggttct cctgcttccg gcgtctgcac tgatgtgggg tctcgcaggc agctcgctcc
     1261 tgtttcttgc gctgttgggt ctggtagctg cacgtgcggg gggcgcgccc gtgattgcat
     1321 ccgtcatgcg cgttgccttt tggggggctc tggcaatggc attgaccgct gcagtggggg
     1381 ccctcttcgg ggtggccgcc tgaattcctg gggccgtgga actcgatgcc tgatcggggt
     1441 aagggactgt cgtctcatgc tgtgcgtggc caatccatta tggtcaccgg tgccagcggc
     1501 ttcatcggcg gacaccttgt ccgccacctg gcagcacttg gctgccgtgt gtcc
//
LOCUS       DATIRT010000299         4499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_636631, whole genome shotgun sequence.
ACCESSION   DATIRT010000299 DATIRT010000000
VERSION     DATIRT010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636631"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VLN25_06810"
     CDS             complement(<1..523)
                     /locus_tag="VLN25_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HSQ10307.1"
                     /translation="MPRPFDLVVFDWDGTVVDSTAMIARSIQKAAQDLDLVVPSMEQA
                     SHVIGLGLKDALAGAVPDLAANRIDEFSDRYRHHYFAGEPDIVLFDGMRELLVGLAEN
                     GARLAVATGKSRRGLDRAFDATGLRVFFASSRCADETNPKPHPAMLLELTEELAVPRA
                     RTVMIGDTTHDLAM"
     gene            complement(507..1457)
                     /locus_tag="VLN25_06815"
     CDS             complement(507..1457)
                     /locus_tag="VLN25_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HSQ10308.1"
                     /translation="MSHVRVSADYAGQRLDNFLIRLAKGVPKSHVYRIVRSGEVRVNK
                     GRVAADYRVQEGDDIRVPPLRTAQPGDAPRPAMAPALMPPILYEDDDLVVIDKPAGVA
                     AHGGSGIAHGVIERVRAARPNQAFLELVHRLDRETSGILMLAKSRRALLSLHEQLREG
                     RVDKRYLVLVAGDWVNDRQHVRLALTKYVNHGGERRVAVDPEGMASHTIFKLKERLEG
                     FSLLEAELRTGRTHQIRVHLSHLGFPIVGDDKYGDFELNRRMARGEFGARLGRMFLHA
                     CATRLTHPVTGERLALLAALPCDCGGILKALKSRHHATTL"
     gene            2161..>4499
                     /locus_tag="VLN25_06820"
     CDS             2161..>4499
                     /locus_tag="VLN25_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HSQ10309.1"
                     /translation="MKRMLFNATHAEELRVGIVDGQKLIDIDIETAGREQRKSNIYKG
                     VITRVEPSLEACFVDYGEERHGFLPFKEVSRSYFKEGVDVRSASIKEALREGQELLVQ
                     IEKEERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEDRQELRDTMDKLELP
                     AGMSIIARTAGIGRTAEELQWDLNYLLKLWHAVEGAAKPQFELSVDDPDSAGKKVTTY
                     VDSNTGSDGQRLKRANPAPFLIVEESNLVIRAIRDYFHPEIGEILVDTDDIYEQARQF
                     MAHVMPDNVARVKRYKDDVPLFSRFQIEHQIETAYSRQVPLPSGGAIVVDHTEALVAV
                     DVNSARATKGSDIEETATRTNLEAADEVARQLRLRDLGGLIVIDFIDMEESKNQKAVE
                     NRLKDALHYDRARVQMGKISRFGLMELSRQRLRPALNEGSHVTCPRCNGVGVIRDTES
                     SALHILRILQEEAMKESTAAVHAQVPVEVAAFLLNEKRGDIAKLEARLRVSIVLIPNK
                     FLDTPHYHVERLRHDDPRLDDVKASFERAAELAPSTDTPYTRGKVEEERARPKQEAVV
                     KGIVPETPAPIVAPPVLVSVPALRVDERPHRGLWGRIVAFFAGEPAAVAKPVTVTPSP
                     TAPRPPREAEPRGDGRRGRGRDQRDGRRDDKRGERKDAQRGDKRTERQGSEGGRAERE
                     ARPARDEAATARQQPERDGRRPQERRGERKPKAAPLEDVTVAPIGVAEAVGDISPISP
                     VPASASLVTPDAATADATSDEHRGKRRRRRRGRGGRGGEG"
BASE COUNT          838 a         1479 c         1458 g          724 t
ORIGIN      
        1 ccatcgcgag atcgtgcgtc gtgtcgccga tcatcacggt ccgggctcga ggcaccgcga
       61 gttcctccgt gagttcgagc agcatcgccg gatgcggctt tggattcgtc tcgtcagcgc
      121 agcgtgacga tgcaaagaac actcgcagcc ccgtcgcgtc gaacgcacgg tcgagtccgc
      181 gacggctctt gccggtggcc acagccagcc gggcgccatt ctctgcgaga ccgaccagca
      241 gttcacgcat tccatcgaac aggacgatat cgggctcgcc ggcaaagtaa tggtgtcggt
      301 aacggtcgct gaattcgtcg atccggttcg ctgccaggtc aggcaccgcg ccggccaacg
      361 cgtccttcag gccaagcccg attacatggc tggcctgctc catcgaggga acgaccaggt
      421 cgaggtcctg tgcggccttc tggatggaac gggcgatcat cgcggtcgag tccaccacgg
      481 tcccgtccca atcgaaaacc accagatcaa agggtcgtgg catggtgccg ggacttcagt
      541 gccttcagaa tgccgccgca atcgcacggc aacgccgcca gcaaggccaa ccgttccccg
      601 gtgacaggat gtgtcaaacg cgttgcgcag gcatgcagga acatgcgccc gagtcgcgcg
      661 ccgaactcgc cgcgcgccat ccggcggttg agttcgaagt caccatactt gtcgtcgccg
      721 acgatcggaa atcccagatg cgacaaatgg acccgaatct ggtgagtgcg tcctgtcctc
      781 agttccgcct cgagcagtga aaatccttcg agccgctcct tgagcttgaa tatcgtgtgc
      841 gatgccatgc cctccgggtc gaccgcaacg cgacgctctc ctccgtggtt cacgtacttc
      901 gtcaatgcaa gccggacatg ttggcgatcg ttgacccagt caccggccac cagcacgagg
      961 tagcgcttgt cgacgcggcc ttcacgcagc tgctcgtgca gggacagcag ggcgcgccgg
     1021 gacttcgcca gcatcaggat gcccgaggtt tcgcgatcca ggcgatgcac gagttccagg
     1081 aacgcctggt ttggacgtgc ggcgcgcacc cgctcgatga ccccgtgcgc gataccgctg
     1141 ccgccatgag cggcaacccc ggccggcttg tcgataacga ccagatcgtc gtcttcgtac
     1201 agaatcggcg gcatcagagc cggagccatg gccggtcgcg gtgcgtcccc cggctgcgcg
     1261 gtccgaagcg gcggcacgcg aatgtcatcg ccttcctgaa cgcggtagtc ggcggccacg
     1321 cgccccttgt tcacgcgtac ctcgccactc cggacgatcc gatacacatg gcttttggga
     1381 acgcccttgg ccaggcggat cagaaagttg tcgaggcgct ggccagcgta atcggcgctg
     1441 acgcgcacgt ggctcacgcg atcggccgcc acaccgtcgg actcttgccg tggcggtgcg
     1501 ccggcttcct gccgctttcc ggttttacgg gcagcgccca tcttgcatat acttgagccg
     1561 tcgaggtttt tcgggactcc cggaggaccc cggggcccct tcggctccgg cgggcgccag
     1621 tctggcgcgc ctgggagcgc aaggcggact ctggttcgcc gaccggaaat cctcacacgc
     1681 gaagaattgt agcgttggca gtcaccgctc cgcggtgcct tcaggcgacg attcaatgca
     1741 ctggtgcccg cgggatgaac tgtcgcgcgg gttttcgagg caacaatgcc tctttgaatt
     1801 cgcagtgagg tcgccgaaag gcggctgcgg cggccttcgc cgactgcgtg ggcccgcgtt
     1861 ttgcgggacc tcgccggaat gaacgtgtgg aactgagcgc ggcgcatggc gccgcgagat
     1921 ctcgagaaca gagtttcgga gccctgcaag tcatgccaac tgtgctttcc ctcccgctca
     1981 ggtcgcccgc tatgggtgcg gccgggacgg cgcgcgcagt cagccaggaa tccggaagac
     2041 cgcccttcgc ggtcatcgtt gtgcgtcgct gcgtcgaccg cacagcctga ttctcgaggc
     2101 cctccccgcc cgtccgtccc ggcaccttga agcgcccctg agaaggcgca aggagtgctg
     2161 atgaaacgca tgcttttcaa cgccacgcat gcagaggaac tgcgcgtggg catcgtcgac
     2221 ggccagaagc tgatcgacat cgacatcgag accgcaggcc gcgagcagcg gaagtcgaac
     2281 atctacaaag gcgtgatcac gcgggtcgag cccagcctcg aggcgtgttt cgtcgactac
     2341 ggcgaggagc gccacggctt cctgcccttc aaggaggtct cccggtccta tttcaaagaa
     2401 ggggtcgacg tccgcagcgc cagcatcaag gaagcgttgc gcgaaggcca ggaactgctg
     2461 gtccagatcg agaaggaaga gcgcggtaac aagggcgccg cgctcacgac cttcatctcg
     2521 ctggcaggcc ggtatctcgt gctgatgccg aacaaccctc gcggcggcgg cgtatcgcgc
     2581 cgcatcgagg gtgaagaccg gcaagaactg cgcgacacca tggacaagct cgagctgcct
     2641 gcgggtatgt cgatcatcgc gcgcaccgcg ggtatcggcc gcacggccga ggaactccag
     2701 tgggacctca actatctgct caagctgtgg catgcggtcg aaggcgctgc caagccgcag
     2761 ttcgaactgt cggtcgacga tccggacagc gcgggtaaga aggtaacgac gtatgtggac
     2821 agcaacaccg gttccgacgg ccagcggctg aagcgggcca atccggcgcc cttcctgatt
     2881 gtcgaagagt cgaatctcgt catccgtgcg atccgcgatt acttccatcc ggagatcggc
     2941 gagatccttg tcgacaccga cgacatctac gaacaggcac gccaattcat ggcgcatgtg
     3001 atgccggaca acgtcgcgcg cgtgaagcgc tacaaggacg acgtgccgct cttttcgcgc
     3061 ttccagatcg agcaccagat cgagaccgcc tattcgcggc aggtcccgct gccctcaggc
     3121 ggcgcgatcg tggtcgatca caccgaggcg ctggtagccg tcgacgtcaa ctcggcccgc
     3181 gccaccaagg gctccgacat cgaggaaacg gcaactcgca cgaatcttga agcggccgac
     3241 gaagtcgcgc gccagctccg tctgcgcgac ctgggcggac tgatcgtcat cgacttcatc
     3301 gacatggagg agtcgaagaa ccagaaggcg gtcgaaaacc gcctcaagga cgcgttgcac
     3361 tacgaccgtg cccgcgtgca gatgggcaag atttcgcgat tcggcctgat ggaactgtca
     3421 cgccagaggc tgcgtccggc gctgaacgaa ggtagccatg tcacgtgtcc gcgctgcaac
     3481 ggcgtcggcg tgatccgcga cacagaaagc tcggcgctgc acatcctgcg catcctgcag
     3541 gaagaagcca tgaaggaaag cacggccgcc gtgcatgcac aggtgcccgt cgaggtcgcg
     3601 gccttcctgc tcaacgagaa gcgcggcgac atcgccaagc tggaagcgcg actgcgggtg
     3661 tcgattgtgc tgatccccaa caaattcctc gacactccgc actatcacgt cgagcgcctg
     3721 cgtcacgatg atccgcggct ggacgatgtc aaggccagct tcgaacgtgc cgccgaactg
     3781 gcgcccagca ccgacacgcc ctacacgcgg ggcaaggttg aggaagagcg cgcacgcccg
     3841 aagcaggagg ccgtggtcaa gggcatcgtg cccgaaaccc cggcgcccat cgttgcgccg
     3901 ccggtgctgg tgtctgtacc ggcactccga gtggacgagc ggccgcaccg cgggctgtgg
     3961 ggtcgaatcg tcgccttctt cgccggtgaa ccggccgccg tagccaagcc ggtgacagtc
     4021 actccgtccc ccaccgcacc gcggcctccg cgcgaagccg agccccgagg cgatgggcgt
     4081 cgcggccgtg gccgggatca acgcgatggc cggcgcgacg acaagcgtgg cgaacgcaag
     4141 gacgcgcagc gtggcgacaa gcgcactgag cgccagggca gcgaaggcgg tcgcgccgaa
     4201 cgtgaagcac gccctgcgcg ggacgaggcc gcgacggcac gccagcaacc cgagcgcgac
     4261 ggtcgcaggc cacaggaacg gcggggagag cgcaagccca aggcagcgcc actcgaagac
     4321 gtcacggtcg ccccgatcgg tgtggccgaa gcagtgggcg acattagtcc gatcagtccg
     4381 gtccctgcga gtgcctcttt ggtcacgccc gacgcggcca ctgccgacgc aacctcggat
     4441 gagcatcgtg gcaagcggcg ccggcgtcgc cgcggtcgtg gtggccgtgg tggcgaagg
//
LOCUS       DATIRT010000300         1956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1816747, whole genome shotgun sequence.
ACCESSION   DATIRT010000300 DATIRT010000000
VERSION     DATIRT010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1816747"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..100
                     /locus_tag="VLN25_06825"
     CDS             <1..100
                     /locus_tag="VLN25_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HSQ10310.1"
                     /translation="EKQKAGKKRMKQVGTVEIPQEAFLAILQVEDK"
     gene            97..987
                     /gene="lepB"
                     /locus_tag="VLN25_06830"
     CDS             97..987
                     /gene="lepB"
                     /locus_tag="VLN25_06830"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HSQ10311.1"
                     /translation="MNFALILFVLTIFTDILWVADKMVFAKQRRARAAKLLADFDSRN
                     AAALARKETIVEQERRSLEDGAVRQPAWLEYTASFFPVILLVFLLRSFLYEPFRIPSG
                     SMLPTLEVGDLILVNKYEYGIRLPVINKKVIDVGQPKRGDVMVLRFPLNPSQDYIKRV
                     VGLPGDRIEYFNKRLTVNGREVPMKKLDNYYDPDRMQYYGRFDEVLGDSGHRVIVNEA
                     APPGLGGGFPNTHPGACQYSGSGLVCTVPAGHYFMMGDNRDNSEDSRFWGFVPDQNIV
                     GRAFLIWMNFGNIGRIGSFH"
     gene            1024..1407
                     /locus_tag="VLN25_06835"
     CDS             1024..1407
                     /locus_tag="VLN25_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HSQ10312.1"
                     /translation="METLRTRRRQRGLSIIGLIFIGLIVVGLLSLGFRTVPAVIEYIA
                     IERAVQKITSEGATVSEIKAAFDRHATIDDITSIKSSDLDITKEGDRIVVSYAYTKRI
                     PILDNVALVIDFAGTTRDRQRKAIP"
     gene            1432..>1956
                     /gene="rnc"
                     /locus_tag="VLN25_06840"
     CDS             1432..>1956
                     /gene="rnc"
                     /locus_tag="VLN25_06840"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HSQ10313.1"
                     /translation="MELAALEERLGYTFVQHTLLEQAVTHRSHGAIHNERLEFLGDAV
                     LNCAIAQILFQKYLRLDEGDLSRLRANLVKQQSLADIAERLVLSDFMRLGEGEMKSGG
                     FRRPSILADTMEAIFGAIFLDSGFDAAKSVIGKLFDPVLKSVDPKTLGKDSKTLLQEY
                     LQGKRIALPVYTVVE"
BASE COUNT          409 a          573 c          613 g          361 t
ORIGIN      
        1 cgaaaagcag aaagccggca agaagcgcat gaagcaggtc ggcacggtcg agattccgca
       61 ggaagccttc ctcgcgatcc tgcaagtgga agacaaatga acttcgcatt gattctgttc
      121 gtcctgacga tcttcaccga catcctgtgg gtcgccgaca agatggtgtt tgccaagcag
      181 cggcgtgccc gcgcggcgaa gctgctggcc gacttcgaca gccgcaatgc cgccgcgctg
      241 gcacgcaagg agaccatcgt cgaacaggag cggcgcagcc tggaggacgg tgcggtccgg
      301 cagccggcat ggctcgagta caccgccagc ttcttcccgg tgatcctgct ggtgttcctg
      361 ctgcgttcgt tcctctatga gccgttccgg attccgtcgg gatcgatgct gccgacgctc
      421 gaagtgggcg atctcatcct ggtcaataag tacgaatacg gcatccggct gccggtgatc
      481 aacaagaagg tgattgacgt cggccagccg aagcggggcg acgtcatggt gttacgcttc
      541 ccgctcaacc cctcgcagga ctacatcaag cgcgtcgtcg gattgccggg cgaccgcatc
      601 gagtatttca acaagcgcct gacggtcaat ggccgcgagg taccgatgaa aaagctcgac
      661 aactactacg atccggaccg gatgcagtac tacggtcggt tcgacgaggt cctgggtgac
      721 agcggtcacc gcgtcattgt caatgaggcg gcacccccgg gactgggcgg tggatttccg
      781 aacacgcacc ccggagcctg tcaatacagt ggcagcgggc tcgtctgcac ggttccggcg
      841 gggcactatt tcatgatggg tgacaatcgg gataacagcg aagacagccg cttctggggc
      901 ttcgtgccgg accagaacat cgtcggacga gcctttctca tctggatgaa ctttggcaat
      961 atcgggcgta taggtagctt tcactgaggg tcatttgctt aagtgactga aaagaaaggg
     1021 aaaatggaaa cgttacgaac tcggcggcga cagcgcggac tcagcatcat cggcctcatc
     1081 ttcatcgggc tgatcgtggt gggcctgctt tctctgggat tcaggaccgt tccggcggtc
     1141 atcgaataca tcgcgatcga gcgcgcggtg cagaagatca cgagtgaggg agccacggtc
     1201 agcgagatca aggccgcctt cgaccggcac gcgacgatcg acgacatcac gtcgatcaag
     1261 tccagtgatc tggacatcac gaaggaaggc gatcggatcg tcgtcagcta cgcgtacaca
     1321 aagcggatcc cgatcctgga caacgtggca ctcgtcatcg atttcgccgg cacgacgcgc
     1381 gaccgccagc gcaaagcgat cccgtgacgg gcgggccgag gattcgatcc gatggaactc
     1441 gcggcgcttg aagagcgcct tggctacacc ttcgtccagc acacgctgct cgaacaggcc
     1501 gtcacgcatc gcagccacgg cgcgatacac aacgaacgcc tcgagtttct cggtgacgcg
     1561 gtcctgaact gcgcgatcgc gcagatcctg ttccagaagt acctgcggct cgacgaaggc
     1621 gacctttcgc ggttgcgtgc caatcttgtc aagcagcagt cgctggcgga catcgccgag
     1681 cgcctggtgc tgtcggactt catgcgcctg ggcgaaggtg aaatgaaaag cggcggtttc
     1741 cgccgcccct cgatcctcgc cgacacgatg gaagcgatct tcggcgcgat cttcctggac
     1801 agcgggttcg acgcggccaa gagcgtgata ggcaagcttt tcgatccggt gctcaagtcc
     1861 gtggatccga agacgctggg caaggacagc aagacgctgc tgcaggagta cctgcagggc
     1921 aagcgcatcg cgttgccggt gtacaccgtg gtcgag
//
LOCUS       DATIRT010000301        14291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_341831, whole genome shotgun sequence.
ACCESSION   DATIRT010000301 DATIRT010000000
VERSION     DATIRT010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 14291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14291
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341831"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VLN25_06845"
     CDS             <1..206
                     /locus_tag="VLN25_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HSQ10314.1"
                     /translation="LATGGADLMLDPTLAHWDVAAILPVIEAAGGVVSGWTGGDPLAE
                     MSLIASAGDLHDEVLRLLHGAGD"
     gene            complement(203..1363)
                     /locus_tag="VLN25_06850"
     CDS             complement(203..1363)
                     /locus_tag="VLN25_06850"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HSQ10315.1"
                     /translation="MSEDLLDFRKSQSLTMGVELELQLIDRRTGDLTRAASDLIALVT
                     RKPFPGDIKPEITESMLEVSTAVHATYSPLRAELLAMRDALVVGADRLDIEVAGGGAH
                     PFQRWYDRQIYDRPRFKHVAGLYGYLAKQFTVFGQHVHIGCDDGTQALRLLHRLSIYI
                     PQFIALAASSPYFQGVDTSFDCSRLNSIYAFPLSGRAPFLTDWSAFETYFAKLRRLGV
                     VESMKDFYWDIRPKPEYGTVEVRVFDTPLSVDRAAALAAYLQAICRMLLDREETVTEE
                     IYLAYNYNRFQACRFGLEGQVLDSVTGESTPLHIAVLRTLEVCRPYARELGSTDALDE
                     IERWIRTDGNDARWQRETMQQRHSLAELVLESADRWRGRAPVTPAGRSTPES"
     gene            complement(1360..2574)
                     /locus_tag="VLN25_06855"
     CDS             complement(1360..2574)
                     /locus_tag="VLN25_06855"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HSQ10316.1"
                     /translation="MDLSFLPDWPPAFPAAVAIAALALLAAAFGEIAARWLRAPRLLG
                     YLAAGALFGAGGYALRALNMEQLPTSTLQFSLEFAAAIILFDLGQRVSFGWIRRNPAL
                     LGASALESGLTFFLVFAVMRWLDVAPLPAALIATISMSTSPAVVLAVTREVRAQGQVT
                     ERVLLLTALNSIYAVVLSTLLLAWARLDTRGLVDDFLLHPAYLIFGSLAIAAVGARLF
                     HLVSAFAGRDRAAQLILMLAVVSIVFATSVALKLSPLLALLACGAFVRTFDRGRRLAS
                     SDFGLISALALMLFFALSAATVDFGILASAGMPALALVLVRSACKVAAVGLLAPFTGI
                     GWRKGVFAGLGLLPMSAIALMLTQQLSQLHPQLGAEAGAVLLASVIVFQVIGALTLLF
                     ALKSSGEAKEDR"
     gene            complement(2822..3301)
                     /gene="ssb"
                     /locus_tag="VLN25_06860"
     CDS             complement(2822..3301)
                     /gene="ssb"
                     /locus_tag="VLN25_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HSQ10317.1"
                     /translation="MASVNKVILIGNLGRDPETRYTADGAAITNIAIATSDRWKDKAT
                     GEMKEATEWHRVVFFGRQAEIAGEYLKKGRPVYVEGRLRTRKWQDKDGNDKYTTEIVA
                     ENMQMLGSREGMGGGGAEFEGAGEESRAAAPARASSGRPPAAKPATTIADMDDDIPF"
     gene            3489..6338
                     /gene="uvrA"
                     /locus_tag="VLN25_06865"
     CDS             3489..6338
                     /gene="uvrA"
                     /locus_tag="VLN25_06865"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HSQ10318.1"
                     /translation="MDEIRIRGARTHNLKSVDLDLPRERLIVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSSYARQFLQLMEKPDVDLIEGLSPAIAIEQRASSHNPRSTVGTVT
                     EIHDYLRLLFARVGTPCCPEHKVPLQSQSVSQMVDSTLALPEDSKILLLAPVVRERKG
                     EFIEFFDDMQTQGFVRFRIDGAIVEADSVPPLAKGEKHSLDVVVDRLKVRADAKQRLA
                     ESFETALRLADGRALLIDLDNGQEITFSSRFACPVCSYSLAELEPRLFSFNNPMGACE
                     ACDGIGTVEYFDEARVVAHPELALASGAIKGWDARNQYYHQMLVTVAQHYGFDLNQPF
                     DQLPESAQRVILHGSGDVDIPFTYTNERGRKMVRRHPFEGVIPSLERRLKETDSLVVR
                     DELSKLRSQRACTVCDGSRLRREARNVFIGEGKEQRGIWQISAAPLAEASRYIETVAF
                     SGQKKEIASKIVKEIASRLRFLVNVGLDYLSLDRSADTLSGGESQRIRLASQIGSGLT
                     GVMYVLDEPSIGLHQRDNARLIGTLKHLRDLGNSVIVVEHDEDAIRAADFIVDMGPGA
                     GAHGGEVIAHGTLAEIEANVNSLTGQYMAGRREIEIPSDRTPPFTHWLRLVGAQGNNL
                     KDVTLDLPIGLLTCITGVSGSGKSTLINDTLYAAVARHLYGSAAEPAHYTMLEGLEHV
                     DKVISVDQSPIGRTPRSNPATYTGLFTPIRELFAETSTARERGYEAGRFSFNVKGGRC
                     ESCQGDGVIKVEMHFLPDVYVPCDVCHAKRYNRETLEVHYKAKSIADVLDMTVEDAFA
                     FFNAVPTIARKLQTLLDVGLGYVRLGQSATTLSGGEAQRVKLSLELSKRDTGRTLYIL
                     DEPTTGLHFHDIALLLKVLHQLRNAGNTVVVIEHNLDVIKTADWVVDLGPEGGDGGGR
                     IIATGPPEKIAASRESHTGAYLAPVLNGERRSRAA"
     gene            complement(6371..6931)
                     /locus_tag="VLN25_06870"
     CDS             complement(6371..6931)
                     /locus_tag="VLN25_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HSQ10319.1"
                     /translation="MTTLRVMLLLALLLGGVRGADADEAAWSALRDGGVLVLRHAQTE
                     PGIGDPPGFRLEDCATQRNLSPAGRAQAAAIGRVLAERRVPVARVESSLWCRCLDTAR
                     LAFPALEVQPVVSLNSFFEDRSAESEQTREVRARIAAWRGPGALVLVTHQVNISALAA
                     RLAAMGEGIVLQRSGADVRVVGSVRF"
     gene            complement(6928..8910)
                     /locus_tag="VLN25_06875"
     CDS             complement(6928..8910)
                     /locus_tag="VLN25_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HSQ10320.1"
                     /translation="MTSGLEVTLVLLASVVLGVVVFRLLSLPPLLAYLAIGILIGPHA
                     LGLVPDSKQTRYLAEFGVVFLMFSIGLEFSLPRLKSMRRIVFGLGGAQVTLTILVTMI
                     GGVLLGKTFGIGFAGVFALGAALAMSSTAIVLKLISERLQLDTEHGKRIVGVLLFQDL
                     AVVPLLVLIPALAADPGQLGGTLGLAVVKATALLTVLLAGGQRVLRWWLHLVAKRKSH
                     ELFTLNVLLVTLGLAWLTEIAGLSLALGAFVAGMLIAETEFRHQVDEDIKPFRDVLLG
                     LFFVTIGMLLSPRVVVEHFGWVLLALVVPVAFKFGLIYFLARLFKAGAGTAIRAALAL
                     CTAGEFGFVLLGLAGQFNLIDDRILQVVLAAMLLSMLAAPLLVQHADRIAMRLASSEW
                     LQKSLEIAQIAQKAIVSDRHVVICGFGRSGQHLAKMLEQENIGYVALDLDPDLVREAA
                     AAGESVVFGDAARRETLVAAGIRRAAALVITYADATSALKVLRHVHELKPNLSVVVRT
                     QDDHDLDRLMKAGATEVVPEVFEGSLMLGSHALVLLGVPVARVVRRVREARNRRYRLL
                     RGYFHGIGDDDGSPEDESHERLHSVPLDVGAAAVGRRLADLELATIGADVTAVRRRGI
                     RGADPSPEMRLQVGDVVVLRGVPESIELAEERLLQG"
     gene            9067..10062
                     /locus_tag="VLN25_06880"
     CDS             9067..10062
                     /locus_tag="VLN25_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HSQ10321.1"
                     /translation="MPATPMPTEPSASALALAREVIEIEAHAVAALADRIDGAFARAI
                     GLLLDCRGRTVVTGVGKSGHIGRKLASTLASTGTPSMFVHAAEAAHGDLGMITQDDVL
                     LALSYSGEGDELLTIVPVVKREGTPLIAMTGHPESSLARLADVHLNVHVDKEACPLNL
                     APTSSTTAMLALGDALAIACLDARGFGREDFARSHPAGALGRRLLTLVRDVMRSGDAI
                     PHVASTASVMEALHEITRKQLGMTAVLDERGALIGIFTDGDLRRLLERVGDVRTLAVA
                     DVMTRTPLTIGPDALAAEAAQLMDRRRRNQLLVVSGDGRLVGALNNLDLMNAKVI"
     gene            10062..10577
                     /locus_tag="VLN25_06885"
     CDS             10062..10577
                     /locus_tag="VLN25_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HSQ10322.1"
                     /translation="MTPEQRAAQVRLVVLDVDGVLTDGRLYYGPQGETLKVFDVRDGH
                     GIKMLLGHGIDVAILSARSSPIVAQRARELGIARVLQGRGDKSAGFRELLEASNVAAA
                     EAAFVGDDLPDLPVLLQAGFAATVDDARDEVKAVAHWVAPQPGGRAAVRALAEFVLRA
                     QGVDPTTMVHG"
     gene            10707..11303
                     /gene="lptC"
                     /locus_tag="VLN25_06890"
     CDS             10707..11303
                     /gene="lptC"
                     /locus_tag="VLN25_06890"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HSQ10323.1"
                     /translation="MRDRITAIIAVLLLALLAATTYWYSQSTRIGGLSNPVSREEPDV
                     VVDGATLTQFDAQGRATNKLIGDRVMHYPSDDRVEVIRPRMISLRADQPQLDARANHA
                     RVEDGGARVVLTGDVTVIRAAGKDGEPPMRLSTERLVALPDVEQFSTDAPAEIERGGS
                     RINSVGMDYDNIKRTVKFRSKVRGTVEANPKAKGVESR"
     gene            11300..11923
                     /gene="lptA"
                     /locus_tag="VLN25_06895"
     CDS             11300..11923
                     /gene="lptA"
                     /locus_tag="VLN25_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HSQ10324.1"
                     /translation="MIRRLIPLLLIALTLTAATGAARAESADRDKPLNYEAASGECDD
                     LQQVCVLVGDVVLVKGTMRATGERVQIRKDPEGYQYGVIQGAPGKVATFRQRRDSSQP
                     GTEEFVEGSAERIEYDEKADTVKLTSRARVRLLENDVQRDELRGDAITYDQRHSKYFV
                     SGGKPSGDANSPDGRVRGTIAPRSNAPKSGSAAPLKPATQIANPRAD"
     gene            11938..12729
                     /gene="lptB"
                     /locus_tag="VLN25_06900"
     CDS             11938..12729
                     /gene="lptB"
                     /locus_tag="VLN25_06900"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HSQ10325.1"
                     /translation="MEAPTRVVDAASLPSRIQSTLSVENLQKRYGNRAVVKDVSLTVQ
                     SGEVVGLLGPNGAGKTTVFYMVVGLVPLDDGDILVDGKSISHLPIHRRARLGLSYLPQ
                     EASVFRKLTVEENVRAVLELQIDDNRRPLPRSVIDNRLTELLDDLQIAHLRTNTALSL
                     SGGERRRVEIARALASRPRFILLDEPFAGVDPIAVLEIQRIVRFLKERGIGVLITDHN
                     VRETLGICDHAYIINEGVVLAAGRPDEIVGNETVRRVYLGEHFRM"
     gene            12869..>14291
                     /locus_tag="VLN25_06905"
     CDS             12869..>14291
                     /locus_tag="VLN25_06905"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HSQ10326.1"
                     /translation="MKPSLQLRLSQHLALTPQLQQSIRLLQLSTLELNQELEQAMSDN
                     PLLERDDDPLGGTLHLRSDGSIENASTTPATPEINGNEPENAAPEFSDTASDASVDWG
                     RGEARTDDDESSSTDWATTRPSLQDHLRSQLSCTQASPRDRALVDLLIEALDTDGYLQ
                     QSLEELLAVCPESVEVDADELRGALRLLQSFDPPGVGARDAAECLGLQLDAMARDDRI
                     FPGAALARRIVSEHLPLLAARDFLRLKRALGCLDDELRAAHQLIRTLNPRPGVAFGDT
                     RADYVVPDVFVRRIKGHWTAVLNAEVMPRLRVNQAYADIFKRGRGDRTVDPAQRGQWT
                     TRIQEARWLIRNIQQRFDTILRVSQAIVDRQHNFFTHGEIAMRPLVLREIADTVGLHE
                     STISRVTANKYMATPFGVLELKHFFGSHVATEAGGAASSTAIRALIKQLIGDEETRNP
                     LSDSKLAELLGEQGIVVARRTVAK"
BASE COUNT         2657 a         4835 c         4553 g         2246 t
ORIGIN      
        1 cactggccac gggcggtgcc gacctgatgc tggatccgac acttgcgcac tgggatgtcg
       61 ccgcgatctt gccggtcatc gaggcggcgg gcggcgttgt cagcggctgg accggcggcg
      121 atccgctcgc agaaatgtca ctgatcgcca gcgccggcga tttgcacgac gaagtgctgc
      181 gcctgctgca cggcgccgga gactagcttt ccggcgttga tcgtcccgcc ggcgtgaccg
      241 gcgcgcgtcc ccgccatcgg tccgccgact cgagcacgag ttctgcgagc gaatgccgct
      301 gctgcatcgt ttcgcgctgc cagcgcgcat cgttgccgtc ggttcgaatc cagcgctcga
      361 tctcgtcaag cgcatcggtc gaaccgagtt cacgcgcgta gggtcggcac acctcaaggg
      421 tgcgaagcac ggcaatgtgc agcggggtcg attcgccggt cacgctgtcc agcacctgcc
      481 cttccagtcc gaagcggcac gcctggaaac ggttgtagtt gtaagcgagg tagatctcct
      541 cggtgaccgt ctcctcgcga tcgagcagca tcctgcagat cgcctgtaga taggcggcaa
      601 gcgccgccgc gcggtccacg gacagcggcg tatcgaacac ccgcacctcg accgtgccgt
      661 actcgggctt tggccggata tcccagtaga agtccttcat gctttcaacg acgcccagcc
      721 ggcgcagctt ggcgaagtac gtttcaaacg ccgaccagtc ggtcaggaac ggcgcacggc
      781 ccgagagcgg aaatgcatag atgctgttga ggcgcgagca gtcgaacgac gtgtcgacgc
      841 cctggaagta cgggctgctc gccgccagcg cgatgaactg cgggatgtag atcgagagac
      901 gatgcagcag tcgcagcgcc tgcgtgccgt cgtcacagcc gatatgcacg tgctgtccga
      961 acaccgtgaa ctgcttggcc aggtagccgt acaggccggc gacgtgcttg aagcgcggtc
     1021 gatcatagat ctggcggtcg taccagcgct ggaacggatg cgcgccaccg cctgcgacct
     1081 cgatatcgag gcggtcggca ccgaccacca gtgcatcgcg catcgccagc agttcggcgc
     1141 gcagcgggga atacgttgcg tgcacggcag tgctgacctc gagcatgctt tcggtgatct
     1201 cgggcttgat gtcgccagga aacggcttgc gcgtcacgag cgcgatcaga tccgacgccg
     1261 cgcgcgtcaa atcgccggtg cgcctgtcga tcagctggag ctcgagctcc acacccatcg
     1321 tcagcgactg cgacttcctg aagtcgagaa gatcttcgct catcgatcct cctttgcctc
     1381 accgctcgac ttcagggcga acagcagcgt cagtgctccg atgacctgga acacgatcac
     1441 gctcgccagc aacacggcgc cggcctccgc acccagctgc ggatggagtt gtgaaagctg
     1501 ctgcgtcagc atcagcgcga tcgccgacat cggcaggagg cccagcccgg caaacacgcc
     1561 cttgcgccag ccgatgcccg tgaagggcgc gagcaggccc accgccgcca ccttgcaggc
     1621 cgaacgcacc agcaccaggg ccagtgccgg catacccgcg cttgcgagga tgccgaagtc
     1681 gaccgtcgcg gccgacagcg cgaagaacag catcaatgcc agcgccgaga tcaggccgaa
     1741 atcactgctc gcgagccgcc ggccgcggtc gaaggtgcgg acgaacgcgc cgcacgccag
     1801 cagcgcgagc agcggcgaca gcttcagcgc gaccgacgtg gcgaagacga tcgacaccac
     1861 cgcgagcatc aggatgagct gggcggcccg atcccgaccg gcgaacgccg acaccaagtg
     1921 gaatagccgc gcaccgaccg ccgcgatagc gagcgacccg aagatcagat acgccgggtg
     1981 tagcaggaag tcgtctacca ggccgcgcgt atcgagccga gcccaggcca gcagcaatgt
     2041 cgacagcact accgcataga tcgagttcaa tgccgtcagc agcagcacgc gttccgtcac
     2101 ctgaccttgc gcccggactt cgcgcgtgac agccagcacg acggccggcg aggttgacat
     2161 gctgatcgtg gcgatcagcg cggcgggcaa gggcgccacg tccagccagc gcatcacggc
     2221 aaacacgaga aagaaggtga gcccgctctc gagggcactg gccccgagca gggccggatt
     2281 gcgacggatc cagccgaagg aaacgcgctg accgagatcg aacaggatga tcgcggcagc
     2341 aaactccagc gagaactgga gcgtgctggt cggcagctgc tccatgttga gggcccgcag
     2401 tgcgtaaccg ccggcaccga acagtgcacc ggctgcgaga tagccgagca ggcgcggcgc
     2461 gcgcaaccag cgcgcagcaa tctccccgaa ggcggccgcc agcaacgcca gcgcggcgat
     2521 cgcgaccgcg gcggggaagg caggaggcca gtcgggaagg aaactgagat ccatcggatt
     2581 gagtcgaagt ggacccgcag caggccgcgg gagccttgga atcggagcgg tcgccacggc
     2641 ggtacacgca gcggctgaag ctatgaacgg cgcacaaagc agggcggcgc cgggacagag
     2701 ggccgacctt agcggcgcga ccccgacagc aggaataccg gttatcgcgt acggagaatt
     2761 actgacatcc cgtgcttgcc cacatcaatg ccgcgtcacg acctgcgcat cgataaccgg
     2821 cctagaacgg gatgtcgtcg tccatgtcag cgatggtcgt cgcgggcttg gcggccggcg
     2881 gccggccgct gctggcgcgc gccggcgccg cagcgcgcga ctcctcgccc gcgccctcga
     2941 attccgcacc gccgcccccc atgccttcgc gcgacccgag catctgcatg ttctcggcca
     3001 cgatctcggt cgtgtacttg tcgttgccgt ccttgtcctg ccacttgcgc gtgcgcagac
     3061 gaccctcgac gtacaccggg cgtcctttct tcaggtactc gccggcaatc tcggcctggc
     3121 gaccaaagaa gaccacgcgg tgccactcgg tcgcttcctt catctcgccg gtcgccttgt
     3181 ccttccagcg gtcggacgtg gcgatggcga tgttggtgat cgctgcgcca tcggccgtgt
     3241 agcgcgtctc ggggtcgcgc ccgagattgc cgatcagtat gaccttgttg acggatgcca
     3301 tagtgttgtc ctccggtgcg ggcggcgctc cccgcccgag ggtctgcaaa cccggctgga
     3361 acgacgccgg gttgcagatc tatctgaaga gggagcgtct tcttgccgcg aggcactgcc
     3421 cacaggcagg tcgcgacgac atttgtcgct atagtaacaa gttcgccttc tgcagctgcc
     3481 cctgaccgat ggatgaaatc cgcatccgcg gcgcccgcac ccacaatctc aagagcgtcg
     3541 acctcgacct gccgcgcgag cggctgatcg tcatcaccgg gctgtccggc tcgggaaaat
     3601 cgtcgctcgc cttcgacacg ctgtacgcgg aaggccagcg gcgctacgtc gaatcgctgt
     3661 cgtcctacgc gcgccagttc ctgcagctga tggaaaagcc cgacgtcgac ctgatcgagg
     3721 gtctgtcgcc cgccatcgcg atcgaacagc gcgcctcgtc acacaatccg cgctcgaccg
     3781 tcggcaccgt gacggagatc cacgattacc tgcggttgct cttcgcccgc gtcggcacgc
     3841 cctgctgtcc cgagcacaag gtgccgctgc agtcgcaaag cgtgtcgcag atggtcgact
     3901 ccacgctcgc gctgcccgaa gactcgaaga tcctgctgct ggccccggtc gtgcgcgagc
     3961 gcaagggcga gttcatcgag tttttcgacg acatgcagac gcaggggttc gtgcgctttc
     4021 gcatcgatgg cgcgatcgtc gaggccgaca gcgtgccgcc actggcgaag ggcgagaagc
     4081 actcgctcga cgtcgtcgtt gaccggctca aggtgcgtgc ggatgcgaag cagcgactgg
     4141 ccgagtcgtt cgagaccgcg ctccgcctgg cggatggccg ggcgctgctg atcgatctgg
     4201 ataacggcca ggagatcacc ttttcaagcc gcttcgcgtg cccggtatgc agctactcgc
     4261 tggccgaact cgaaccacgg ctcttctcgt tcaacaatcc gatgggcgcg tgcgaggcct
     4321 gtgacggcat cggcacggtg gagtacttcg acgaagctcg cgttgtggcg caccccgagc
     4381 ttgccctcgc cagcggcgcg ataaagggct gggatgcgcg caaccagtat taccaccaga
     4441 tgctggtgac cgtggcccag cactacggct tcgacctgaa ccagccgttc gaccagctgc
     4501 cggaatcggc gcagcgggtc atcctgcacg gttccggcga cgtcgatatt ccgtttacct
     4561 acaccaacga gcgcggccgc aagatggtac gtcgccatcc gttcgaaggc gtgatcccga
     4621 gcctcgagcg ccggttgaag gaaaccgatt cgctggtcgt gcgcgacgag ctgtcgaagc
     4681 tgcgcagcca gcgtgcctgc accgtgtgcg atggatcgcg cctgcggcgc gaagcgcgca
     4741 acgtcttcat aggcgagggc aaggagcaac gcgggatctg gcagatctcg gcagcgccac
     4801 ttgcggaggc ctcgcgctac atcgagaccg tcgccttcag cggccagaag aaggagattg
     4861 ccagcaagat cgtcaaggag atcgcctcgc gcttgcgctt cctggtcaat gtcgggctgg
     4921 actacctttc gctcgaccgc tcggccgaca cgctgtccgg cggcgaatca caacgcatcc
     4981 gcctggcatc gcagatcggg tcgggcctga ccggtgtgat gtacgtcctg gacgagccct
     5041 cgatcggcct gcaccagcgc gacaacgctc gcctgatcgg cacactcaag cacctgcgcg
     5101 acctcggcaa cagcgtgatc gtggtcgaac acgacgagga tgccatccgc gccgccgact
     5161 tcatcgtcga catgggaccg ggtgcggggg cgcacggggg cgaagtgatc gcgcacggga
     5221 cgctggccga aatcgaagcc aacgtgaatt cgctgaccgg ccagtacatg gccggccgtc
     5281 gcgagatcga gattccgtcg gaccggacac cgccgttcac gcactggctg aggttggtgg
     5341 gcgcgcaggg caacaacctc aaggacgtta cgctcgacct gccgatcggc ctgctgacat
     5401 gcatcaccgg cgtctcgggg tccggcaagt cgacgctcat caacgacacg ctgtacgccg
     5461 ccgtggcccg gcatctgtac ggctcggcgg cggagcctgc gcactacacg atgctcgagg
     5521 gactggagca cgtcgacaag gtgatcagcg tcgaccagag cccgatcggg cgcactccgc
     5581 gcagcaaccc ggccacctac acggggctct tcacaccgat ccgtgagctg tttgcagaaa
     5641 cgtcgacagc acgcgagcgt ggctacgagg ccggccggtt ttcgttcaac gtcaagggcg
     5701 gccggtgcga gagttgccag ggcgatggcg tcatcaaggt cgagatgcac ttcctgcccg
     5761 acgtttatgt gccctgcgac gtatgccacg ccaagcgcta caaccgcgag acgctcgagg
     5821 tgcactacaa ggccaagagt atcgccgacg ttctggacat gacggtcgaa gacgccttcg
     5881 cgttcttcaa tgccgtgccg acgatcgcgc gcaaattgca gacgctgctc gacgtcggcc
     5941 tcggctacgt acggctaggc cagagcgcca ccacgttgtc aggcggagaa gcgcagcgcg
     6001 tgaagctctc acttgaactg tccaagcgcg ataccggccg tacgctctac atcctcgacg
     6061 agccgaccac cggcctgcac ttccacgaca tcgccctgct gctgaaagtg ctgcaccagt
     6121 tgcgcaacgc cggcaacacc gtggtcgtca tcgagcacaa cctggatgtg atcaagacgg
     6181 ccgactgggt ggtcgatctc ggtcccgaag gcggcgacgg cggtggacgc atcatcgcga
     6241 ccgggccgcc ggaaaagatt gccgccagtc gcgaaagcca caccggcgcc tatctggccc
     6301 cggtgctcaa cggcgagagg cggtcgcgcg cagcctgacg gcgggggctc tcccaccctc
     6361 tgcgatcgaa tcagaagcgg actgagccga ccacgcgcac gtccgcgccg ctgcgttgga
     6421 gcacgatccc ttcgcccatg gcggccaggc gtgcggccag tgcgctgata ttgacctggt
     6481 gtgtcaccag cacaagcgcg cccgggccgc gccacgcggc gatgcgcgca cgcacctcgc
     6541 gtgtctgctc agactcggcg gatcgatcct cgaagaacga attgagcgac acgaccggtt
     6601 gcacctcgag cgcgggaaac gcgaggcgcg cagtatcgag gcaacggcac caaaggctcg
     6661 actcgacgcg tgcgaccgga acacgccgct cggcaagcac gcgaccgatg gcggctgcct
     6721 gcgcccgccc cgccggcgag aggttgcgct gggtggcaca atcctcgagc cggaaacccg
     6781 gcggatcgcc gatgccgggt tcggtctgcg catgacgcag cacgagcact ccgccgtcgc
     6841 gcaaggcgct ccacgccgcc tcgtcagcgt cggccccgcg cacgcctccg agcaggagtg
     6901 cgagcaacag cattacgcgg agcgtcgtca tccctgcagc agcctttcct cggccagctc
     6961 tatcgactcg ggtacgccgc gcaaaacgac gacatcgccg acctgcaatc tcatttccgg
     7021 gctcggatcg gcaccacgga taccgcgccg acggaccgcc gtcacatcgg cgccgatcgt
     7081 cgccagttcc agatcggcga gccgcctgcc gaccgccgcg gcaccgacat cgaggggcac
     7141 cgaatgcagc cgttcgtgcg actcgtcctc gggcgagccg tcgtcgtcac cgatgccgtg
     7201 aaagtagccc cgcagcagcc ggtagcgccg gttccgtgcc tcgcgcacac gccggacaac
     7261 gcgtgcgacc ggcacgccga gcagcacaag ggcgtgggag cccagcatca ggctaccctc
     7321 gaatacttcc ggcaccacct cggtcgcgcc tgccttcatc agtctgtcga ggtcatggtc
     7381 gtcctgcgtg cgtacaacga cggacagatt cggcttgagc tcgtgcacat ggcgcagcac
     7441 cttgagggcc gaggtcgcat cggcgtaggt gatcacgagc gccgccgccc gccggatccc
     7501 ggctgcgacc agggtttcgc gccgtgccgc gtcaccaaag accaccgact caccggctgc
     7561 ggcggcttcc cgcaccaggt ccggatcgag gtcgagtgcc acgtagccga tgttctcctg
     7621 ctccagcatc ttggccagat gctgcccgct gcgaccgaat ccgcaaatga ccacatgccg
     7681 gtcgctgacg atcgccttct gcgcgatctg ggcgatttca agcgacttct gcaaccactc
     7741 gctggacgcc agccgcatcg cgatgcgatc cgcatgctgc accagcagcg gcgcagcgag
     7801 catcgacaac agcatcgccg ctagcactac ctgcaggatc cggtcgtcga tcaggttgaa
     7861 ctgtccggcc aggcccagca gcacgaaccc gaattcgccc gctgtgcaca gtgccagcgc
     7921 ggcccggatc gccgtacccg cgccggcctt gaaaagccgc gcaagaaagt agatcaggcc
     7981 gaacttgaaa gcgacgggta ccacgagagc gagcaggacc cagccgaagt gctcaaccac
     8041 gacacgcggg ctcaacaaca tcccgatggt cacgaagaac aatccgagca gcacgtcccg
     8101 gaacggcttg atgtcctcgt cgacctgatg tcggaattcg gtctcggcga tcagcatgcc
     8161 ggcaacgaag gctccaaggg cgagtgacag gccggcaatc tcggtcagcc aggcgaggcc
     8221 gagcgtgacc agcagcacgt tgagcgtgaa aagctcgtgc gatttgcgct tcgccaccag
     8281 atgcagccac cagcgcagca cgcgttgccc gcctgccagc agcaccgtca gcagcgccgt
     8341 ggccttcact acagcgagtc ccagggtccc gcccagctgc cccgggtcgg ccgccagcgc
     8401 cggaatcagc accagcagcg gaaccaccgc cagatcctgg aacagcagta cgccgacgat
     8461 gcgcttgccg tgctcggtgt cgagctggag ccgctccgaa atgagcttca ggacgatcgc
     8521 ggtggacgac atcgccagcg ccgctcccag cgcgaagacg ccagcgaagc cgataccgaa
     8581 cgttttgccc agcaggactc cgccgatcat cgtgacgagg atggtcagcg tgacctgcgc
     8641 gccaccgagc ccgaacacga tccgccgcat cgacttcagc cgaggcagcg agaattcgag
     8701 cccgatcgag aacatcagga aaacgacgcc gaactccgcc agatagcgcg tctgcttgct
     8761 gtcggggacg aggcccagcg catgtgggcc gatgagtatc ccgatcgcga gataggccag
     8821 cagaggcggc agcgacagca accggaacac aaccaccccc agcaccacag aggcaagcag
     8881 aaccagggtg acttccaggc cagacgtcat cgagcgggca atggtgcaga gaaatggatc
     8941 ggtcgcgcgg cgcaagcaaa gcccatgcca gcacgcatcg cgcgtgtatt atcgccgcag
     9001 cctacccgta gccttcccac gtggccttgc ctgccgccat tcactccgat tcagcttccg
     9061 acttccatgc cagcgacacc gatgcccacg gaaccgagcg cgagcgcgct cgcgctggcg
     9121 cgcgaggtga tcgagatcga ggcccatgcg gtcgccgcgc tcgccgaccg catcgatggg
     9181 gccttcgccc gcgccatcgg cctgctgctg gactgtcgcg gccgcacggt ggtgaccggg
     9241 gtcggcaaga gcggccatat cggtcgcaag ctggcgtcga cgctcgcctc caccggaacg
     9301 ccgtctatgt tcgtccacgc tgccgaggcg gcccacggcg acctcgggat gatcacgcag
     9361 gacgacgtgc tgctggcact gtcgtattcg ggtgaaggcg acgaactgct gaccatcgtg
     9421 ccagtcgtga agcgggaagg cactccgctg atcgcgatga ccgggcaccc ggagtcgtcg
     9481 ctggcgaggc tcgccgatgt acacctcaac gtgcatgtcg acaaggaagc ctgtccgctc
     9541 aatctcgcgc ccacctcctc caccacggcc atgcttgcgt tgggggacgc cctggcgatt
     9601 gcctgcctcg acgctcgcgg attcgggcgc gaggacttcg cgcgctcgca tccggccggc
     9661 gcactcggac gccgattgct gaccctcgtg cgcgacgtca tgcgcagcgg cgatgccatt
     9721 ccacacgtgg cgagcaccgc cagcgtgatg gaagccctgc acgagatcac gcgcaagcag
     9781 ctgggcatga cggcagtgct cgatgaacgc ggcgcgttga tcggcatctt caccgacggc
     9841 gatctgcgac gactgctcga gcgcgtcggc gatgtgcgca cactcgctgt tgccgacgtg
     9901 atgacgcgta cacccttgac gatcggaccc gacgcgctcg ccgccgaagc ggcgcagctg
     9961 atggatcgcc ggcgcaggaa ccagttgctc gtcgtcagtg gcgacggacg gctcgtcgga
    10021 gcgctcaaca atctcgacct gatgaatgcg aaggtgatct gatgacgccc gagcagcgcg
    10081 cggcacaggt ccgcctggtc gtccttgacg tcgacggtgt gctgaccgac ggacggcttt
    10141 actacggacc acagggcgaa acactcaagg tcttcgacgt gcgtgacggc cacggaatca
    10201 agatgctgct cggccacggc atcgacgtag cgatcctgtc ggcgcgcagt tcacctatcg
    10261 tggcacagcg tgcccgagaa ctcggcatcg cccgtgtcct tcagggccgg ggcgacaagt
    10321 cggccggctt ccgtgaactg ctcgaggcat caaacgtcgc tgccgccgaa gccgcctttg
    10381 tcggcgacga cctgcccgat ctgccggtgc tgctgcaggc cggattcgcg gcaactgtcg
    10441 acgacgcccg tgacgaagtg aaggccgtcg cgcactgggt cgcgccgcag cccggcggcc
    10501 gtgccgccgt tcgcgccctt gccgagttcg tcctgagggc ccagggagtc gacccgacga
    10561 cgatggtgca cggatgagcc ctagccgagg tttcgtgccg tttttcttgc cgagggcgcc
    10621 ctgccgcccg aagccctcgg cacacttatc agcgcgccga gcgcacgtgc ggcgcgaagc
    10681 gctgcgtacg cgaggcgcgt gcagctatgc gtgaccgcat caccgccatc atcgccgtat
    10741 tgctgcttgc actgcttgca gcgacgacct actggtactc gcaatccacg cgcatcggcg
    10801 gcttgtcgaa cccggtgtca cgcgaggagc ccgacgtggt ggtcgatggc gccacgctca
    10861 cgcagtttga cgcgcaaggc cgggccacca acaagctcat cggtgaccgt gtcatgcact
    10921 atcccagcga cgaccgggtc gaggtgattc gcccgcgcat gatcagcctg cgcgcggacc
    10981 agccccagct ggatgcacga gccaatcatg cacgcgtgga ggatggcggc gcgcgcgtgg
    11041 tccttaccgg cgatgtcacg gtgatccgtg cagcgggcaa ggacggcgaa cctccgatgc
    11101 gcctgtcgac agagcggctg gttgcgctgc ctgacgtcga gcagttctcg accgatgctc
    11161 cggcggagat cgagcgcggc ggctcgcgca tcaactcggt tggcatggac tacgacaaca
    11221 tcaagcggac cgtgaagttc cgctcgaagg tccggggcac cgtcgaagcc aaccccaaag
    11281 cgaagggagt cgagtcccga tgatccgccg gctgatccca ctcctcctta tagccctgac
    11341 gctcaccgcc gccaccggtg ctgcgcgcgc cgagagcgcc gaccgcgaca agccgctcaa
    11401 ctacgaagcc gccagtggcg agtgtgacga cctgcaacag gtctgcgtgc tcgtcggcga
    11461 tgtcgtactc gtcaagggca cgatgcgcgc caccggcgag cgcgtgcaga tacgcaagga
    11521 ccccgagggt taccagtacg gcgtgatcca gggcgcgccc ggcaaggtgg ccacgttccg
    11581 gcagcggcgc gattcgagcc agcccggcac cgaggagttt gttgaagggt cggcggagcg
    11641 tatcgagtac gacgagaagg cagacaccgt gaagctgacc tcgcgtgcgc gcgtgcgtct
    11701 gctcgaaaac gatgtccagc gcgatgaact gcgcggcgac gcgatcactt acgaccagcg
    11761 acattccaag tacttcgttt cgggtggcaa gccctccgga gacgccaaca gccccgacgg
    11821 ccgggtgcgc ggcacgatcg cccctcgttc gaatgcacca aagtccggtt ccgctgcgcc
    11881 gctgaaaccg gcgacccaga tcgcaaatcc gcgggccgat tgacgagccg atccatcgtg
    11941 gaagccccga ctcgcgtcgt cgacgccgcc tccctgcctt cacgaattca aagcaccctg
    12001 agtgtcgaga acctgcagaa gcgctatggc aatcgcgctg tcgtaaagga tgtctcgctg
    12061 accgtgcaaa gcggagaagt cgtcggcctg ctcgggccta atggggcggg caagaccacg
    12121 gttttctaca tggtggtggg gctggtaccg ctggacgacg gtgacatcct ggtcgacggc
    12181 aagtcgattt cgcacctgcc catacaccgg cgcgcgcgcc tcggcctgtc ctacctgcca
    12241 caggaagcgt cggttttccg caagctgacg gtcgaagaga acgttcgcgc cgtgctcgag
    12301 ctgcaaatcg acgacaaccg acgaccctta ccccgcagcg tcatcgacaa tcgactgacc
    12361 gagctgcttg acgatctgca gatcgcccat ctgcgaacca ataccgccct gtcgttgtcg
    12421 ggcggcgagc ggcgacgcgt cgagatcgcc cgcgcgctgg cgtcgcgccc gcgcttcatc
    12481 ctgctcgacg agccgttcgc gggcgtcgat cccatcgccg tgctggagat tcaacgcatc
    12541 gtgcgcttcc tcaaggagcg cggcatcggc gtgctgatta ccgatcacaa cgtgcgcgaa
    12601 acactcggga tctgcgacca cgcttacatc atcaatgaag gtgtcgtgct cgctgccggg
    12661 cgacccgacg agatcgtcgg caacgagacc gtgcgccgcg tctatctcgg cgagcacttc
    12721 cggatgtgac gatgtgccac gccgcgcgcc cgtcttctgt agttgtcctg cgcgcgaccg
    12781 cccggccgcc cgaagggcgc acgcctgatc aacccgcggg cgatgcacgc gcgacccgcc
    12841 gggttgcgac cttcgcgggc acccgcgcat gaaacccagc cttcagcttc gcctgtcgca
    12901 gcatctggcg ctgacgccgc agctgcagca gtcgatacgc ctgctgcagc tctccactct
    12961 ggagctgaat caggaactcg agcaggcaat gtctgacaac cccctcctcg agcgcgacga
    13021 cgatcccctg ggaggcacgc tgcacctgcg ctccgacgga tcgatcgaaa atgcgtccac
    13081 gactcccgcg acacccgaga tcaatggcaa cgaaccggag aacgcagcac ccgagttttc
    13141 ggacaccgcg tcggacgcca gtgtcgactg gggtcgcggc gaggcgcgaa ccgacgatga
    13201 tgaatcgagt tcgacggact gggccaccac ccgtccgtcg ctgcaggatc atctgcgcag
    13261 tcaactctca tgcacccagg cgagcccgcg cgaccgcgcc ctggtcgacc tgctgatcga
    13321 ggccctcgat accgacggct atcttcagca gtcgctcgag gaactgctcg cggtctgccc
    13381 ggaaagcgtc gaagtcgatg ccgacgaact ccgcggcgcc ctgcgcctgt tgcagagctt
    13441 cgatcctccg ggagtcggtg cgcgcgacgc tgccgaatgc ctcggcctgc agcttgacgc
    13501 catggcgcgt gacgaccgca ttttcccggg ggcggcgctg gcacgacgca tcgttagcga
    13561 gcacttgcct ttgctggcag cccgggattt cctgagattg aagagggccc tgggctgctt
    13621 ggacgatgag ctacgtgcag cgcaccaact gattcgcaca ctcaatccgc ggccgggtgt
    13681 agccttcggt gacacgcgcg cggactatgt cgttccggac gtatttgttc gacggatcaa
    13741 agggcactgg acagcagtat taaatgcgga agtgatgccg cgtttgcgag tcaaccaggc
    13801 ctatgccgat atcttcaagc ggggacgggg cgaccgcacc gtcgatccgg cccaacgcgg
    13861 gcagtggacg acgcgcatac aggaagcacg gtggctgatt cgcaatatcc agcagcgctt
    13921 cgacaccatc ctgcgggtgt cccaggcgat cgtcgaccgg cagcacaact tcttcacgca
    13981 tggcgaaatc gccatgcgtc ccctcgttct cagggaaatt gccgatacgg tgggactgca
    14041 cgaatccacc atttcgcgtg tcacagccaa caagtacatg gccacgccgt tcggcgtgct
    14101 ggaactcaaa catttcttcg gcagccatgt ggcgaccgaa gccggcggtg cggcgtcgtc
    14161 cacggctatc cgcgccttga tcaagcaact gataggagat gaagaaaccc ggaatccact
    14221 ctcggacagc aagctcgccg aactgctggg cgaacagggc atcgtggtgg cgcggcgtac
    14281 cgtcgcgaag t
//
LOCUS       DATIRT010000302         2050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1325018, whole genome shotgun sequence.
ACCESSION   DATIRT010000302 DATIRT010000000
VERSION     DATIRT010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325018"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..199)
                     /locus_tag="VLN25_06910"
     CDS             complement(59..199)
                     /locus_tag="VLN25_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3149 domain-containing protein"
                     /protein_id="HSQ10327.1"
                     /translation="MEALKLLFTSDIGLMSLFTIVAIIGMAVWFRRFFNRQIEEEART
                     RR"
     gene            complement(239..637)
                     /locus_tag="VLN25_06915"
     CDS             complement(239..637)
                     /locus_tag="VLN25_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HSQ10328.1"
                     /translation="MPLTPQQLETLHACLRALRMRVGGAVSDRLHRHGLELHEDAALP
                     NRREDTDDDAAAESATTMDIAHVARDAEELAVVDAALARIAAGEYGQCVDCDEDIARE
                     RLSANPSAARCTECQERSERAAMVARRTRA"
     gene            887..>2050
                     /locus_tag="VLN25_06920"
     CDS             887..>2050
                     /locus_tag="VLN25_06920"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HSQ10329.1"
                     /translation="MSMDSVHYLRPAMVPRSIAVVGASPRHDSLGRFVYTNVMAAGFK
                     GEVYAVNPRHRTIDGHPCLPSLAALPTRVDLAVVVTGAQNVPGIIDEAGKKGIPAMLV
                     LSPGLCEDGPERRQLEREVVERARAARVRVLGPNCLGLMRPDIGLNASWAKTMARPGA
                     LALVSQSGAVTTALLDYAWTAGFGFSSVLATGAESDVSLAEILDYLAGDAATRSILLY
                     VEGIEDARPLLSSIRIAASVKPVIVLKAGRHAAGARVVMSHTGMLVGNDAVFDAALRR
                     VGAVRVPKIGHLFAAAEALASGRLPRGNRLAIVTNGSGPGALAADAADDNHVELARLT
                     DATRAALDAALPPGTSHANPVNVLADADAERFATALKLLVDDRASDGVLVLFAP"
BASE COUNT          307 a          689 c          697 g          357 t
ORIGIN      
        1 gcgcaaagcc gaccctgccg accgcccgga tgcgcgggcg gcgtgcaacc gccaggactc
       61 agcggcgcgt gcgcgcctct tcttcgatct gcctgttgaa gaagcgacgg aaccagacgg
      121 ccatgccgat gatggccacg atagtgaaaa gggacatcag cccgatgtcg ctggtgaaca
      181 gcagcttcag tgcttccatg aattctccgt ggcgaccggt ttcagcggcg tcgtggcgtc
      241 aggcgcgggt gcgccgcgcg accatcgccg cgcgttcgct gcgctcctgg cattcggtgc
      301 agcgtgcggc cgacgggttg gccgacagcc gctcgcgcgc gatgtcctcg tcgcagtcga
      361 cgcactggcc gtactcaccc gcagcgatgc gcgcgagggc cgcgtccacg acggccagct
      421 cctccgcgtc acgcgcgaca tgcgcgatgt ccatcgtcgt cgccgactcg gccgctgcgt
      481 cgtcgtcggt gtcttcgcgg cggttcggca gcgccgcatc ctcgtgcaat tcgagcccgt
      541 gccgatgcag gcgatcggac acagcgcctc ccacacgcat gcgcaaggcg cgaaggcacg
      601 cgtgcagtgt ctcgagttgc tggggggtga gcggcatacg gtattccgga cgggaggcat
      661 gtggcgcctt cggagtgttc tcctgacgcc tcgggttcag cctaaaggtc ggcgtcgacg
      721 cgtgcttgac gcgtatcaac gccgtttcga ccctgcccat cattgcgacc ggcgcgaatc
      781 tgataacgtg ccttccgttg ctgcaatgcc acatccgggc ctcgccgcta tcgacgagtg
      841 ccatggcccg gtctgatctg actcttacga atccacacgc cgcccgatga gcatggactc
      901 cgtccactac ctgaggcccg cgatggtgcc gcggtcgatc gcggtcgtcg gtgcgagccc
      961 gcgacacgac tcgctcggcc ggtttgtcta caccaatgtg atggctgccg gcttcaaagg
     1021 cgaggtgtat gccgtcaatc cccggcatcg cacgatcgac gggcatccgt gtctgccgtc
     1081 actcgcagcg ctgccaacgc gcgtggacct tgcggtggtc gtcaccggag cgcagaacgt
     1141 tccgggcatc atcgacgaag ctggcaagaa gggtatcccg gcgatgctgg tgctttcgcc
     1201 gggcttgtgc gaagacggtc ccgagcggcg ccagctcgag cgcgaggtcg tcgagcgcgc
     1261 acgcgcagcg cgggtccgcg tgctggggcc caattgcctg ggtctgatgc ggccggatat
     1321 cggactgaat gccagctggg cgaagacgat ggcgcggccc ggtgcactgg cactggtgtc
     1381 tcagtccggc gccgtgacga cagcgttgct cgactacgcg tggaccgcag gattcgggtt
     1441 ctcgtcggtg ctggcgacgg gcgcggagtc cgatgtctcg ctcgcggaga tccttgacta
     1501 tcttgccggc gacgctgcca cacgttcgat cctgctgtat gtcgaaggca tcgaagatgc
     1561 gcgcccgctg ctgtcgagca tccgcattgc ggccagcgtc aagccggtca ttgtgctcaa
     1621 ggcggggcga cacgcggccg gcgcgcgcgt ggtgatgagc cacaccggca tgctggtggg
     1681 caacgatgcg gttttcgacg ccgccttgcg ccgcgtcggc gcggtccgcg ttccgaagat
     1741 cgggcacctg ttcgccgcag ccgaggcact tgccagcggg cgactgccgc gcggaaaccg
     1801 cctcgccatc gtcaccaacg gcagcgggcc cggtgcgctg gcggccgacg cggccgatga
     1861 caaccacgtg gaactcgcgc ggctgacgga cgccacacgc gctgcgctcg acgccgcgtt
     1921 gccgcccggc acgtcgcacg ccaatcccgt caatgttctc gcggatgcag atgccgaacg
     1981 ctttgccacc gcattgaagc tgctggtcga cgaccgtgcc agcgatggag tgctcgtgct
     2041 gttcgcgccg
//
LOCUS       DATIRT010000303         6218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_735525, whole genome shotgun sequence.
ACCESSION   DATIRT010000303 DATIRT010000000
VERSION     DATIRT010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6218
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735525"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="VLN25_06925"
     CDS             complement(<1..705)
                     /locus_tag="VLN25_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSQ10330.1"
                     /translation="MSAIRHCASALQWLGAIALGVLAGAASAQQALTPIRASEHVWYF
                     QGDSGMASTANRAFNSNAAFVVTRDGVVVFDALGTPELGAAMKRAIEKISAQPIRVVI
                     VSHFHADHFYGLQSLLGPNVEIWASGKARGTVSDAPALERLEQRRLDLWPYVDEKTRL
                     VPASRWIDFTPGRDFAFERGGIRFRLIDVGGAHSPEDIMLWVENDRVLLAGDLYFSGR
                     IPFVGNADTRSWLTTLD"
     gene            complement(702..1655)
                     /locus_tag="VLN25_06930"
     CDS             complement(702..1655)
                     /locus_tag="VLN25_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HSQ10331.1"
                     /translation="MPEHHPIEVRPPDITAYRESPSGVDYVHVFDSGRPGPTVMLQAL
                     THGNEFCGAHALIEVLESGIRPQAGRLIVAFANVEAFAGFDFDDPDASRYVDEDYNRV
                     WADPVLDGPRDSSELRRARALRPFVDAADFLLDLHSMHEPGRPIMVCGMVDKHLELAR
                     KVGMPADLLIDTGHPAGLRMRDRGGFNDSASTKQALLIECGQHWEQRAVDVARDTTLR
                     FLAAAGSVPFDRIAARLTEPLPAAQRVVRVTEPVVARSAAFEFLVPIEGLGIVPKVGP
                     PIARDGDKVWVAPYDDTVLVMPSMRHLKPGNTQVRLGRYDS"
     gene            complement(1648..2508)
                     /locus_tag="VLN25_06935"
     CDS             complement(1648..2508)
                     /locus_tag="VLN25_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HSQ10332.1"
                     /translation="MTSEACSLHGPDQPRVALVLDSPHSGVDFPPDFDAAVSEFDLRD
                     GEDCFVDELYLPAIEHGIPLLAARYPRTYLDPNRHHGDIDLDLIEGGTWPHEYLPSGK
                     AAIGKALIWRTLDDGRSIYSRKLRVDEVRSRIDRFHAPYHRTLSQLLATAHEDFGVVY
                     HLNCHSMNSVAGKMGEGGEGTERADFVLGDRDGTTCDPSFTEFIRSTLAAMGYVVAVN
                     DPYKGVELVRAYSDPARGRHSLQVEINKRLYMDEPARARHSGFAPLQANLMHLLDAIR
                     DYIRREHRRA"
     gene            complement(2553..3593)
                     /locus_tag="VLN25_06940"
     CDS             complement(2553..3593)
                     /locus_tag="VLN25_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSQ10333.1"
                     /translation="MSSAAPGRPEQARAAARSAEAFAPALLQIDAVSKRFSKRLDAIE
                     KLARTLGAKVSEHTVHAVDRVSFEVAEREVVGLVGESGCGKSTLGRLACGLHAPTEGT
                     VRYRGQPVTTEAGVRRPIQMIFKDPFASLNPRMRVTDIVGEAPRVHGIIGRDEVADYV
                     AELLQRVGLDPSFARRYPHQFSGGQRARVGIARALAVKPEFLVCDEAVAALDVSIQAQ
                     VLNLFMDLRDRYDLTYLFISHDLGVVRHLSNRVVVMYLGRVVEVAKTEELFAQPNHPY
                     TQALLFEVPRLDRRRRTFVPIRGEIPSPLAPPPGCHFHPRCPSAMPLCATVAPALREI
                     SPGRLSACHLND"
     gene            complement(3590..4555)
                     /locus_tag="VLN25_06945"
     CDS             complement(3590..4555)
                     /locus_tag="VLN25_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSQ10334.1"
                     /translation="MSPLLKVNGLRTEFATRDGVLPAVADISFDLARGEVLGLVGESG
                     SGKSVTGFSILGLIDPPGRIAAGSIVFDGAELVGAAPDSLRRLRGKRIAMIFQDPMMT
                     LNPVLSIETQMVETVLAHDEISRASARDRSIEALGRVGIAAAAERLTQYPHQFSGGMR
                     QRVAIAIALLHKPDLIIADEPTTALDVTVQGQILYEMQKLVRESGTALIWITHDLAVV
                     AGLADRIAVMYAGRIVEMGAAAAVLDAPLHPYSRGLLDSVPAATQPGARLRQIRGSTP
                     SLLRLGPGCAFRDRCDRATDECALMPPLAEHDGRAVRCVHPLEFA"
     gene            complement(4579..5481)
                     /locus_tag="VLN25_06950"
     CDS             complement(4579..5481)
                     /locus_tag="VLN25_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSQ10335.1"
                     /translation="MSVLVAPVPAVETPWQRFRKQFAESHLAMAGLALLVVIIAIALF
                     APWIAPQNPYDLGKLDLLDSRLPPGAQSADGMTYWLGTDGQGRDMLSAILYGLRVSLY
                     VGVASGVIALTLGAVTGVLAAYLGGRFEQIVMRIVDIQLGFPAILVALILLAVLGKGV
                     DKIIIALVTVQWAYYARTARSAALVERSKEYIEAARALGLGAGRIVLRHLLPNALPPL
                     IVVGTVQVAHAIALEATLSFLGLGLPPTEPSLGLLIANGYEVLMSGKYWISVYPGIAL
                     LVTILSINLVGDQLRDVLNPRLSR"
     gene            complement(5478..>6218)
                     /locus_tag="VLN25_06955"
     CDS             complement(5478..>6218)
                     /locus_tag="VLN25_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSQ10336.1"
                     /translation="FVYARPALEVIFERMPATLELAFLAMVIAVTLGIPLGLYAGLRP
                     HSVVSKTIMAGSILGFSLPTFWVGLILILIFAVTLGWLPSTGRGPTTDVLGLKLSIFN
                     WDGLRYALLPAINLALFKLSLIIRLTRAQVREQSLLDYIKFARAKGLTNVRVIGVHLL
                     KNIMIPLVTVIGLELGSVIAFAVVTETIFAWPGMGKLVIDSIFQLDRPVVVAYLMVVV
                     FMFIVINLVVDLLYSVLDPRVRLSEVRG"
BASE COUNT         1151 a         2018 c         2074 g          975 t
ORIGIN      
        1 gtccagcgtg gtgagccagg agcgcgtgtc ggcattgccg acgaacggga tgcgcccgct
       61 gaagtacagg tcgccagcca gcagaacgcg gtcgttctcg acccacagca tgatgtcttc
      121 cggcgagtgc gcgccgccga catcgatcag gcgaaagcgg atgccaccgc gctcgaaggc
      181 aaagtcgcgg ccgggggtga agtcgatcca gcggctggca ggcaccagcc gcgtcttttc
      241 gtccacgtag ggccagaggt cgagccgacg ctgctcgagg cgctccagtg ccggagcgtc
      301 gctgaccgtg ccgcgagcct tgccgctggc ccagatctcc acgtttggtc ccaacagtga
      361 ttgcagtccg tagaagtggt cggcatgaaa gtggctcacg atcacgacgc ggatcggctg
      421 cgcgctgatt ttctcgatcg cccgcttcat ggcggcgccg agttcgggcg tgccgagcgc
      481 atcaaagacg acgacgccgt cacgtgtcac gacaaaggcc gcgttcgagt tgaacgcacg
      541 attggccgtc gaggccatgc ccgagtcgcc ctggaagtac cagacgtgct cggatgcgcg
      601 gatcggcgtg agcgcttgct gagccgacgc ggctccggca aggacaccca gtgcgatggc
      661 accgagccac tgcagggcgg atgcgcagtg tcggatcgcg ctcatgagtc gtagcggccg
      721 agccgcacct gagtgttgcc gggcttcaga tgccgcatcg acggcatcac cagcacggtg
      781 tcgtcatagg gcgcgaccca gactttgtcg ccgtctcggg cgatcggtgg tcccaccttc
      841 ggcacgattc ccaggccttc gatcggcacc aggaactcga aggccgccga ccgcgcgacc
      901 accggctcgg tgacgcgcac gacgcgctgc gcggctggca gcggctccgt caggcgtgcc
      961 gcgatgcgat cgaacggcac gctgccggcg gccgcaagaa aacgcagtgt cgtgtcacgc
     1021 gccacgtcga ccgcacgctg ttcccagtgc tgcccgcatt cgatcagcag cgcctgcttc
     1081 gtgctcgccg aatcgttgaa accgccccgg tcgcgcatgc gcagcccggc cggatgcccg
     1141 gtgtcgatca gcaggtcggc cggcatgccg accttgcgcg cgagttcgag atgcttgtcg
     1201 accatgccac acaccatgat cggacgacca ggttcgtgca tcgagtgcag gtcgagcagg
     1261 aagtcggcgg catcgacgaa gggccgcagc gcgcgggcac gccggagttc cgaggagtca
     1321 cgcgggccgt cgaggactgg gtcggcccag acgcggttgt agtcctcgtc gacatagcgc
     1381 gaggcgtcgg ggtcatcgaa gtcgaagccc gcgaaggcct cgacattggc aaaggcaacg
     1441 atcaggcgac ccgcctgcgg ccggatgccg gattccagca cctcgatcaa cgcgtgcgca
     1501 ccgcagaact cgttgccgtg cgtgagtgcc tgcagcatca ccgtgggacc gggacggccg
     1561 gaatcgaaca catgcacgta gtcgacgccg ctcggacttt ctcggtaggc cgtgatgtcg
     1621 ggcgggcgca cttcgatcgg atggtgttca ggcacggcga tgctcccggc gaatgtaatc
     1681 gcggattgcg tccagcagat gcatgaggtt ggcctgcagt ggggcaaagc cggagtgcct
     1741 ggcccgcgct ggctcgtcca tgtacagacg cttgttgatc tcaacctgca ggctgtggcg
     1801 gccgcgagcc ggatcggagt aggcgcgcac cagttcgacc cccttgtagg gatcgttcac
     1861 cgcgaccacg tagcccatcg cggctagcgt ggaccgtatg aactcggtga acgacggatc
     1921 gcaggtcgtg ccatcgcgat caccgagcac gaaatccgcg cgctccgttc cctcgccgcc
     1981 ttcgcccatc ttgccggcga ccgaattcat cgaatgacaa ttgaggtggt agaccacgcc
     2041 gaagtcctca tgcgccgtcg cgagcagttg cgagagggtc cggtggtacg gcgcatgaaa
     2101 ccgatcgatg cgtgaacgca cctcgtcgac ccgcagcttg cgcgagtaga tcgaacgtcc
     2161 atcgtccagc gtgcgccaga tcaacgcctt gccgatcgcg gcctttccgc tcggcaggta
     2221 ttcgtgcggc caggtgccgc cctcgatcag gtccaggtcg atgtcgccgt ggtggcgatt
     2281 cgggtccagg tacgtgcgcg gatagcgggc cgccagcaac gggatgccgt gctcgatggc
     2341 cggcaggtag agctcatcga cgaagcagtc ctcgccgtca cgcaggtcga attccgaaac
     2401 agccgcatcg aaatccggcg ggaagtcgac gcccgagtgc ggcgagtcga gtaccagcgc
     2461 aacgcgaggc tggtcaggcc cgtggagtga gcaggcttca gatgtcatag gagcgattca
     2521 acgtgaaaga ccgttcctca gtcgttcaaa tgtcagtcgt tcaagtgaca cgccgacaag
     2581 cgaccaggcg aaatctctcg caatgccgga gccaccgtcg cgcatagggg catcgcgctc
     2641 gggcaacgcg gatggaagtg gcaacccggg ggcggtgcca gcggcgaagg gatctcgccc
     2701 ctgatcggga cgaaagtcct gcgcctgcgg tccagccgcg gaacctcgaa cagcaaggcc
     2761 tgcgtatagg gatggttcgg ctgggcaaaa agttcctcgg tcttcgcaac ctcgacgacc
     2821 cggcccaggt acatcacgac aacgcggttg gacaggtgcc gcaccacacc cagatcgtgg
     2881 ctgataaaaa ggtaggtcag gtcgtagcgg tcgcgcagat ccatgaagag gttcaacacc
     2941 tgcgcctgga tcgagacatc gagcgctgcc accgcttcgt cacagaccag gaactctggc
     3001 ttcactgcca gtgcgcgcgc gatcccgacg cgcgcccgct ggccgccgga aaactggtgc
     3061 ggatagcgac gcgcgaacga agggtcgagg ccgacccgct gcagcagctc cgcgacatag
     3121 tcggccactt cgtcccggcc gatgatgccg tgcacgcgcg gggcctcgcc aacgatgtcg
     3181 gtgacgcgca tgcgtggatt cagcgaggcg aacggatcct tgaagatcat ctggatcggc
     3241 cgtcgcacgc ccgcttcggt cgtgacgggc tggcctcgat agcgcaccgt cccttcggtc
     3301 ggtgcgtgca ggccgcacgc aagacgcccg agggtggact tgccgcaacc ggactctccg
     3361 accagcccga cgacttcgcg ctcggccacc tcgaagctca cgcggtcgac cgcgtgcacc
     3421 gtgtgctcgc tcaccttcgc gcccagcgtg cgcgccagtt tctcgatcgc atccagccgc
     3481 ttgctgaagc gcttgctgac ggcgtcgatc tgcagcagcg cgggagcaaa ggcctccgcg
     3541 ctgcgcgctg cggcgcgagc ttgctcggga cggcccggtg ctgcgctgct catgcaaact
     3601 ccagcggatg cacgcagcgc accgcgcggc catcgtgctc ggccagcggc ggcatcagcg
     3661 cgcattcgtc ggtggcgcgg tcgcagcggt cgcggaatgc acagccggga ccgagccgca
     3721 gcagcgaggg cgtcgatccg cggatctgcc gtaatcgtgc gcccggctgt gtggccgccg
     3781 ggaccgagtc gagcaggccg cgcgagtagg gatggagcgg cgcatccagc acggcggccg
     3841 cggcacccat ctcgacgatg cgtccggcgt acatcaccgc gatgcggtcg gcgagtcccg
     3901 cgaccactgc aagatcgtgc gtgatccaga tgagcgctgt gccggactcg cgcacgagct
     3961 tctgcatctc gtagaggatc tgtccctgca ccgtgacgtc gagtgcggtc gtcggctcgt
     4021 ctgcaatgat gagatcgggc ttgtgcagca gcgcaattgc gatggccacg cgctggcgca
     4081 tgccgccgga aaactggtgc ggatactggg tcagtcgctc ggccgccgcg gcgatgccga
     4141 cgcgtcccag cgcttcgatc gagcggtcgc gcgccgacgc acgggagatc tcgtcgtggg
     4201 cgagcacggt ttccaccatt tgcgtttcga tcgacaggac cggattgagc gtcatcatcg
     4261 ggtcctggaa gatcatcgca atgcgcttgc cgcgcagtcg ccgcagcgag tcgggtgccg
     4321 cgccgaccag ttcggcgccg tcgaacacga tgctgccggc ggcgatgcgg cccggcggat
     4381 cgatcagtcc aaggatcgag aagccggtca ccgacttgcc cgaccccgat tcgcccacca
     4441 gtccgagcac ctcgccgcgc gcaagatcga acgaaatgtc cgccacggcg ggcaggacgc
     4501 cgtcacgtgt cgcaaactcg gtgcgcaggc cgttgacctt cagcaagggg ctcatagtct
     4561 tcagcttctg cagcttcgtc agcgcgaaag ccgtgggttg agcacgtcgc gcaactggtc
     4621 accgaccagg ttgatcgaaa ggatcgtgac cagcagcgcg atgcccgggt agacgctgat
     4681 ccagtacttg cccgacatca ggacttcgta gccgttggcg atcagcaggc ctagcgacgg
     4741 ttcggtcggc ggcaggccga gacccagaaa ggacagcgtg gcctcgagcg cgatggcgtg
     4801 cgcgacctgc actgtgccga cgacaatgag cggcggcagt gcgttcggca gcagatgccg
     4861 caggacgatg cgccccgcgc ccagtccgag cgcacgcgcc gcctcgatgt actccttgct
     4921 gcgttcgacc agcgccgccg aacgcgccgt gcgtgcgtag tacgcccact gcacggtgac
     4981 cagcgcgatg atgatcttgt cgacgccctt gccgagcacc gccagcagga tcagggcgac
     5041 caggatcgcg gggaagccga gctgaatatc gacgatgcgc atgacgatct gctcgaagcg
     5101 gccgccgagg taggcggcga gcacgcccgt gacggcgccg agcgtcagcg caatgacgcc
     5161 gctagcgacg cctacataga gcgacacccg cagtccgtaa aggatggccg acagcatgtc
     5221 gcgaccctgg ccgtcagtgc cgagccagta agtcatgccg tccgccgact gcgcgcccgg
     5281 cggcagccgc gagtcgagca ggtccagctt gccaaggtca taggggttct gcggcgcgat
     5341 ccacggggcg aacagcgcga tcgcaatgat caccacgagc agcgccaggc ccgccatggc
     5401 caggtgactt tcggcgaact gcttgcggaa gcgctgccaa ggtgtctcga cggccggcac
     5461 tggcgcgacg aggacgctca tccccttacc tctgacagcc gcacccgcgg gtcgagcacc
     5521 gagtaaagca gatcgactac cagattgatg acgatgaaca tgaacacgac caccatcagg
     5581 taggcgacca cgacgggccg gtcgagctgg aagatcgagt cgatgaccag cttgcccata
     5641 ccgggccacg cgaagattgt ttcagtcacc accgcaaagg cgatcaccga gcccagctcg
     5701 agcccaatca ccgtgaccag aggaatcatg atgttcttca gcaggtgcac gccgatgacg
     5761 cgcacgttgg tcaacccctt ggcgcgggcg aacttgatgt agtcaagcag gctctgctcg
     5821 cgtacctgcg cacgtgtcag tcggatgatc agcgagagct tgaagagcgc caggttgatg
     5881 gccggcagca gcgcgtatct gagcccgtcc cagttgaaga tggatagctt cagcccgagg
     5941 acgtcggtgg tcggaccgcg tcccgtcgac ggcagccatc ccagcgtgac cgcgaagatc
     6001 aggatcagga tcagtcccac ccagaaggtc ggcagcgaga agccgaggat cgatccggcc
     6061 atgatggtct tcgacaccac gctgtgcggt cgcagaccgg cataaagccc aagcggaatg
     6121 cccagcgtga ccgcaatgac catggcgaga aacgcgagtt cgagcgtcgc cggcatgcgt
     6181 tcgaagatga cctctagcgc cgggcgtgca tagacgaa
//
LOCUS       DATIRT010000304         2514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1227597, whole genome shotgun sequence.
ACCESSION   DATIRT010000304 DATIRT010000000
VERSION     DATIRT010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227597"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1601)
                     /locus_tag="VLN25_06960"
     CDS             complement(69..1601)
                     /locus_tag="VLN25_06960"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HSQ10337.1"
                     /translation="MALWLALVLPALPLQLAERALPGMGPLAIVEGPPQRLQVIHCNA
                     PAQAAGVAPGMKVVAAQALARELIALERNVEREQAALAELAGWAYQFSSHVVLQPDGL
                     LLETGGSERLFGDRARLHRAITTGLDTLGYRAAFACADTPRCARVFARAEASQGRHAA
                     SPNLPPLAEADSLRRLPIEFLEWDAVTNETLRALGLSAIGDLLRLPRDAFARRFGTGR
                     LDDLDRALGMRSTPQPLYVPPAHFSARIELPADISETAQLMFPARRLLASLEGFLRGR
                     NAGATELLFAATHSARRSVETAPTTVTLALAAPERDAQRLAALLGERLARVQLPAPAL
                     TLALTVERLHAFAALNASLLPPSAHDGDVGVDWLQLAETLHARLGSERVFQLKAVDDH
                     RPERAWCALPIAVGDESRTAPRSITSAPRPLLLLPTPQPLAAHGEQPLLPPQREPLTL
                     VTGPERIECGWWDVSGPAAGSPARAVHRDYFVARNARGQWLWIYRELAAPRGWFLHGL
                     FA"
     gene            complement(1688..>2514)
                     /gene="imuA"
                     /locus_tag="VLN25_06965"
     CDS             complement(1688..>2514)
                     /gene="imuA"
                     /locus_tag="VLN25_06965"
                     /inference="COORDINATES: protein motif:HMM:NF033429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HSQ10338.1"
                     /translation="HFFRRTHMRAATELLSLLHTAGGASLWRGDERPADETCGPTLAS
                     GFAELDRELPGGGWPQGQLIELLTDDAGIGELSLLAPALAQLAQARRCSVWVLPVDAS
                     RTVSHVPEAQTIPYAPALATAGLDLARTIFVKPATPREGLWAIEQSLRARHLGAVVGW
                     LPSASGTGDGDFRALRRLHLLAQRHQALAFVLRPARHAQAPSPAVLRLLLGSAGTRLQ
                     VTLLKRRGRPLLGPVTLALRPAHWESARVEADTAPRVLPASAALLDTQRPMDALLT"
BASE COUNT          390 a          863 c          941 g          320 t
ORIGIN      
        1 gatgcgccgc gcaggaacga gaagttcgac aggcagtgca gttcggcgta ggagggcgtc
       61 atcgcgtctc aagcaaacag tccgtgcagg aaccagccgc gcggggcggc aagttcgcgg
      121 tagatccaca gccactggcc gcgtgcgttg cgagcgacga agtagtcgcg gtgcacggcg
      181 cgtgcgggtg agccggcggc ggggccggag acatcccacc agccgcactc gatgcgctcg
      241 gggccggtga ccagcgtcag cggctcgcgc tgaggcggca acagtggctg ctctccgtgc
      301 gccgccagtg gctgcggagt cggcagcagc agcagcggac ggggcgccga cgtgatcgac
      361 ctcggtgcgg tgcgcgactc gtcaccgacg gcgatgggca gtgcacacca ggcccgctcg
      421 ggccggtgat cgtcgaccgc cttcagctgg aacacgcgtt cggagccgag ccgcgcatgc
      481 agggtttccg caagctgcag ccagtccacc ccgacatcac cgtcatgcgc ggaaggcggc
      541 agcaggctgg cgttgagcgc cgcgaaagca tgcagccgct cgaccgtgag cgcgagcgtc
      601 agcgccgggg ccggcagctg cacgcgcgcc agccgttcgc cgagcagcgc cgccagtcgc
      661 tgcgcatcgc gctccggtgc ggcgagtgcg agcgtgactg tggtcggcgc cgtttcgacg
      721 ctgcggcgcg cgctgtgcgt ggcggcgaac aagagttcag tcgcgcccgc attgcgcccg
      781 cgcaggaaac cttcgagcga ggcgagcagc cgccgcgccg ggaacatcag ctgcgcggtc
      841 tcgctgatat cggccggcag ttcgatgcgc gccgaaaaat gcgcgggagg cacgtacaga
      901 ggctgcgggg tgctgcgcat tccgagcgcg cggtcgaggt cgtcgagccg ccccgtaccg
      961 aaacgccgcg cgaacgcgtc acgtggcaga cgcagcaggt cgccgatcgc cgacagcccg
     1021 agtgcacgca gtgtctcgtt cgtcactgcg tcccattcga gaaactcaat cggcaggcgt
     1081 cgcagactgt ccgcctccgc gagcggggga aggttcggcg aggcggcgtg ccggccctgg
     1141 ctcgcctctg cccgcgcgaa cacacgtgca cagcgcggag tgtcggcaca ggcgaaggcg
     1201 gcgcgatagc cgagcgtgtc gaggccggtg gtgatcgcgc gatgcagtcg ggcacgatca
     1261 ccgaacagcc gctcgctacc gccggtttcg agcagcagtc cgtcgggctg caacaccacg
     1321 tgcgacgaga actggtaggc ccagccggcg agttcggcga gcgcggcctg ctcgcgttcg
     1381 acattgcgtt cgagcgcgat cagctcgcgt gccagcgcct gtgcggcgac gaccttcatg
     1441 cccggcgcca cgccggccgc ctgcgccggc gcgttgcagt gaatcacctg caggcgctgc
     1501 ggcggtcctt cgacgatcgc gagcggcccc atgccgggca gtgcgcgctc ggccagttgc
     1561 agcggcagcg caggcaggac gagggcgagc cacagggcca cggtgcagcg cgggcgggtg
     1621 cccgccgaag ctcaagtaag tggcatcagg ccggcgcgat caggccagtg ctgtcaggcc
     1681 agcgcgatca ggtcaacagc gcgtccatcg gacgctgcgt gtcgagcagc gcggccgacg
     1741 ccggcagtac gcgcggtgcg gtatcggctt cgacgcgtgc gctttcccag tgtgcggggc
     1801 gcagtgcgag cgtgaccggc ccgagcagcg ggcggccgcg ccgcttcagc agcgtcacct
     1861 gcagccgcgt gccggcgctc cccagcagca ggcgcaatac cgcgggcgac ggcgcctgtg
     1921 cgtggcgtgc cggacgcagc acgaaggcca gcgcctggtg gcgctgtgcc agcaggtgca
     1981 ggcggcgcag cgcgcggaag tcaccgtcgc cagtcccgct ggccgacggc agccagccga
     2041 ccaccgcgcc gagatggcgc gcccgcagcg actgctcgat cgcccacagg ccctcgcgtg
     2101 gcgtggccgg cttgacgaag atcgtgcgcg cgaggtcgag cccagcggtg gcgagcgccg
     2161 gcgcataggg aatcgtctgc gcttcgggca catgcgacac ggtgcgcgag gcatccaccg
     2221 gcagcaccca caccgagcag cggcgcgcct gcgccagttg cgccagtgcc ggcgccagca
     2281 gcgagagttc gccgatgccg gcgtcgtcgg tgagcagttc gatcagctgc ccctgcggcc
     2341 agccgccgcc cggcagttcg cggtcgagtt cggcaaagcc gctggcgagc gtcgggccgc
     2401 aggtttcgtc ggcagggcgc tcgtcgccgc gccacaggga cgcaccgccg gcggtgtgca
     2461 acagggaaag caactccgtg gccgcacgca tgtgcgtcct cctgaaaaag tgga
//
LOCUS       DATIRT010000305         2970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1424210, whole genome shotgun sequence.
ACCESSION   DATIRT010000305 DATIRT010000000
VERSION     DATIRT010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424210"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VLN25_06970"
     CDS             <1..366
                     /locus_tag="VLN25_06970"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HSQ10339.1"
                     /translation="FTDIPTAIPHIKSGKLKALGVTSAQPSQALPDVLPIAQQGRAGF
                     DLKAWYGVLAPANTPKPVIDRLNAEITKALNSPELKEKLLSMGMEPIALDPAQSASYM
                     KTEIDRWANVVKISGAKID"
     gene            465..1313
                     /locus_tag="VLN25_06975"
     CDS             465..1313
                     /locus_tag="VLN25_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSQ10340.1"
                     /translation="MFIDYASKPPIPAFESPAPHLGNYDRVYESSKSVSEAAGPSLAE
                     YLATYERAGARHVVVKAKDVETTFGVKVTNEGVAEFCRQQGGRYIGFAGVDPHKGIAA
                     IRELEQAVRELGLRGLNLQCFEHKLHINDKKMYPLYAKCIELDIPVNIHCSINFSTST
                     LMEYGRPQLLDEVMVHFPELRVCAAPPGWPWVHELIGVAWRHRNVHIGLVAVRPKYLQ
                     QPASGYEGLVQFGGSVLQDRILFGTSYPLVPVERSVREVEELPLKDEVKRKWLHDNAA
                     RFLRLA"
     gene            1337..2413
                     /locus_tag="VLN25_06980"
     CDS             1337..2413
                     /locus_tag="VLN25_06980"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSQ10341.1"
                     /translation="MTMQRDRRKAVSQLLSLSGGVAIGIPRLAFGQAAPPKPASIVVN
                     AAGGAVNKSLREAFFSEFERRYGIKVIDTSPTDFGKLRAMVQSGNVEWSVMEINEADA
                     ERAVKLNLLEPLDMSVINMAGHPKELQDNKYVVTRGIYSTVLGYRTDVFASRPRPTGW
                     AEFWDVKKFPGPRALRSSPVDNLEFALLADGVKPNQIYPIDVDRAFRKMDQIKPHISV
                     WWTTGAQSAQVLIDKEVVLGTAWHGRFYTAIKDGAPIAIDFNEGIIHRTPFGIPRGAK
                     DAYWGQRFLALLTEPQLQAKYANLMSYPGLHQDSIKYTDPAMIPFLPTSANNLSRQVW
                     SSAGWWNDNADAVQERWNRWMLAR"
     gene            2440..>2970
                     /locus_tag="VLN25_06985"
     CDS             2440..>2970
                     /locus_tag="VLN25_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSQ10342.1"
                     /translation="MATVLTLAGVTKHYGEVPALRGVDLEVHSGELLTLLGPSGSGKT
                     TLLKVIAGFEIAEGGVVSLVGRDITTLAPNKREIGMVFQNYALFPHMSLAANIAFPLN
                     IRKVPKQAIARQVTDALQLVGLAGYGERLPKQLSGGQQQRVALARALVNLPSALLLDD
                     PLAALDRKLREAMQIEL"
BASE COUNT          575 a          924 c          956 g          515 t
ORIGIN      
        1 ttcaccgaca ttcccacggc gatcccgcac atcaagagcg gcaagctcaa ggcgcttgga
       61 gtgacgtccg ctcagccttc gcaagctctg ccggatgtcc tgccgatcgc ccagcagggt
      121 cgtgccggat tcgatctgaa ggcctggtat ggagtgctgg cccccgcgaa caccccgaag
      181 ccggtgatcg accggctgaa cgcagagata acgaaggcgc tcaactcgcc tgaactgaag
      241 gagaaactgc tgtcgatggg catggagccg atcgctctcg acccggccca gtcggcgagc
      301 tacatgaaga cggagattga ccgctgggcg aatgtcgtca agatctcggg ggccaagatc
      361 gactagctgc ggcacggctc gatcggccgc gcacgcagca aacccctgcc cgacgcttga
      421 cgtaccggga caccacgcaa acactgcgcc cggaggagaa catcgtgttc attgactacg
      481 cgagcaagcc gccgataccg gctttcgagt cgccggcccc gcatctgggc aactatgacc
      541 gggtctacga atccagcaag tcggtttcgg aggccgccgg tccctcgctg gcggagtacc
      601 tcgccaccta cgagcgtgcg ggtgcccggc atgtcgttgt caaagcgaag gatgtcgaaa
      661 cgaccttcgg cgtgaaggtc accaacgagg gcgtggcgga gttctgccgg cagcagggcg
      721 gccgctatat cggcttcgcc ggtgtcgatc cgcacaaggg catcgcggcg atccgcgaac
      781 tggaacaagc ggtgcgcgaa ctggggctgc gggggctcaa cttgcagtgc ttcgagcaca
      841 agctgcacat caacgacaag aagatgtacc cgctgtacgc caagtgcatc gaactcgaca
      901 ttccggtcaa catccattgt tcgatcaact tctcgaccag cacgctgatg gaatacggcc
      961 gtccgcaact gctggacgaa gtcatggtcc attttccgga gctacgtgtc tgcgcggccc
     1021 cgccgggctg gccgtgggtg cacgaactga tcggcgtggc ctggcggcac aggaacgttc
     1081 atatcggtct cgtcgcggtg cggcctaagt atctgcagca gcccgcctcc ggctacgaag
     1141 gcttggtcca gttcggcggc agcgtgctgc aggaccggat tttgttcggt acctcctacc
     1201 cgctcgtccc ggtcgagcgt tccgtccgcg aggtcgaaga gttgcctttg aaagacgaag
     1261 taaagcggaa gtggctgcac gacaacgccg cgcgattcct gcggctcgca taaccgataa
     1321 ctcgaggaga catgccatga cgatgcaaag ggatcgacgc aaggccgtct cgcaactgct
     1381 ttcgctgtcc gggggtgtcg ccatcggcat cccgcggctt gcctttggcc aggcggcgcc
     1441 gccgaagccc gccagcatcg ttgtcaacgc cgcaggtggc gcggtcaaca agtcgttgcg
     1501 cgaagccttc ttcagtgaat tcgagagacg ctacggcatc aaggttattg ataccagtcc
     1561 caccgacttc ggcaagctcc gcgcgatggt ccagtcgggc aacgtcgagt ggtcggtcat
     1621 ggagatcaac gaggcggacg ctgaacgagc cgtcaagctc aacctgctcg agccgctcga
     1681 catgagcgtg atcaacatgg cggggcatcc gaaggaactg caggacaaca agtacgtcgt
     1741 cacgcgcggc atttattcga cggtgctcgg atatcgcacg gacgtgttcg ccagtcggcc
     1801 gcgcccgacg ggctgggccg agttctggga cgtgaagaaa ttccccgggc cacgggcgct
     1861 gcgcagttcg ccagtcgaca acctggaatt cgcgctgctg gccgacggcg tgaagccgaa
     1921 ccagatctac ccgatcgatg tggaccgtgc gttccggaag atggatcaga tcaagccgca
     1981 catctcggtg tggtggacga ccggcgctca atccgcgcag gtcctgatcg acaaggaggt
     2041 ggtgctcggc acggcgtggc acggccgctt ctataccgcg atcaaggacg gtgcgccgat
     2101 cgccatcgac ttcaacgaag gcatcatcca tcgcacgccc ttcgggattc cgcgcggtgc
     2161 caaggatgcg tactgggggc agcgtttcct tgcgctgctg acggagccgc aattgcaggc
     2221 gaagtacgcg aacctgatgt cctatccggg cctgcaccag gactcgatca agtacaccga
     2281 cccggccatg atcccgttcc tgccgacctc ggcgaacaat ctgtcaaggc aggtgtggtc
     2341 cagcgcaggc tggtggaatg acaacgcgga tgcggtccag gaacgctgga atcggtggat
     2401 gctggcgcgc tagggcaggc ctctcggacc tcacggggcg tggccactgt ccttaccctt
     2461 gcgggagtga ccaagcacta cggtgaggtc ccggccctgc gcggcgtcga tctcgaggtc
     2521 cactcgggcg aactgctgac cctgctcgga cccagcggtt cgggcaagac gacgctgctg
     2581 aaagtcattg ccgggttcga gattgccgaa ggtggagtgg tatcgctcgt cgggcgcgac
     2641 atcacgacgc tcgcgcccaa caagcgcgaa atcggcatgg tgtttcagaa ctacgccttg
     2701 ttcccgcaca tgagcttggc ggcgaacatc gcgtttccgc tgaacatccg caaggtgccg
     2761 aaacaggcca ttgcgcgcca agtgacggac gcccttcagc tggtcggact ggcgggctac
     2821 ggcgagcgcc tgccgaagca actttcgggt gggcagcagc agcgcgtcgc gctggcgcgg
     2881 gcgcttgtca acctgccgag cgcgctgctg ctcgacgacc cgctggcggc gctcgatcgc
     2941 aagctgcgcg aggcgatgca gatcgagctc
//
LOCUS       DATIRT010000306         1714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_441150, whole genome shotgun sequence.
ACCESSION   DATIRT010000306 DATIRT010000000
VERSION     DATIRT010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441150"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1559)
                     /locus_tag="VLN25_06990"
     CDS             complement(54..1559)
                     /locus_tag="VLN25_06990"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HSQ10343.1"
                     /translation="MRWLSRALFWIGLTLGLAAICLAGAISVGVLSNGVLSTAAPAWI
                     AHVHVAGHAVHVNVPGLIRLATAPGVAQLLDGRSIDTPSGVLVFHRVGRAVVAHCSPC
                     RLQHPALANAPLVLPRLELGAERTGATVNGWLAGEGFRVEYTAHLAVDRIRLQWQLPA
                     TDLAALYGALSSVIPEARTARIEGSLHAQGVLELPSRRSSVAFGLAGLEVGGLGTEAL
                     QFGGFRMSCAHAGGTARRVVTGDGEARWVALDRMGYLAAAVIAAEDQRFLEHAGIDEA
                     EVARLLADLDDDGPKRGASTLTQQLARTLYTGGERTAARKLRELLYAVEMERTLGKAR
                     ILELYLNTVDWGPGLCGARSAARLYFRKRPDQLTPLEAAWLAGILRAPHVAHEQQFLA
                     GQHEIERARRVLLQMRELPKSERVRWAGKPLLLAAPAKTAGTTDSPTAAARSPAAPTN
                     PSAQRDADRDAPIAEPALESAKAAGNRRRAVRAAAVVGELMAHDAMTVALD"
BASE COUNT          266 a          607 c          589 g          252 t
ORIGIN      
        1 acgtgcacga ccgcgaaggc ggaaccacgt tctgtccggc gtgtcatgcg gtgctaatcg
       61 agcgcgactg tcatcgcatc gtgcgccatg agctcaccga cgacggccgc tgcccgcact
      121 gcgcgacggc ggttgccggc cgctttggcc gattcgaggg cgggttcggc aatcggcgca
      181 tcccggtccg cgtcgcgctg cgctgacggg ttcgtcggag ccgccggcga acgcgctgcc
      241 gcggtgggac tgtcggtcgt tcctgccgtc ttcgccggcg ccgcaagcag cagcggcttg
      301 ccggcccagc gcacacgctc ggatttgggc agctccctca tctgcagcag cacccggcgc
      361 gcccgctcga tctcgtgctg gcccgcgaga aactgctgct cgtgggcgac atgcggtgcc
      421 cgcaggatgc cggccagcca ggcggcctcc agcggtgtca gctgatcggg acgcttcctg
      481 aaatacaagc gcgccgccga ccgtgctccg cacagtcccg gcccccagtc gaccgtgttg
      541 aggtagagct cgaggatgcg ggccttgccg agggtgcgct ccatttcgac tgcatagagc
      601 agttcgcgca gcttgcgtgc ggccgtgcgt tcgccgccgg tgtagagcgt gcgtgcgagc
      661 tgctgcgtca acgtgctcgc cccgcgcttc ggaccgtcat cgtccagatc ggccagcagc
      721 cgcgccactt cggcttcgtc gatgccggcg tgctcgagga agcgctggtc ctcggccgcg
      781 atgacagccg ccgccaggta gcccatgcgg tcgagcgcca cccagcgcgc ctcgccatcg
      841 ccggtcacca cgcggcgcgc ggtaccgccc gcgtgcgcgc acgacattcg aaacccaccg
      901 aattgcaggg cctcggtgcc gaggccgccg acctccagcc cggccagacc gaaggcgacc
      961 gagctcctgc ggctcggcag ttcgagcact ccctgggcgt gcaacgatcc ctcgatgcgt
     1021 gcagtgcgtg cttcagggat gaccgaggag agcgccccat agagcgcggc cagatcggtc
     1081 gcaggcagtt gccattgcag ccggatgcgg tccactgcga ggtgggcggt gtactcgaca
     1141 cgaaaacctt caccggccaa ccatccgttg acggtggcgc cggtgcgctc ggcgccgagc
     1201 tcgaggcgcg gcagcaccag cggtgcgttg gccagcgctg gatgctgtag gcgacacggc
     1261 gagcagtgcg cgacgacggc ccggcctacg cggtgaaaga caagaacccc ggacggcgta
     1321 tcaatactgc ggccgtcgag cagctgggca actccgggcg cggtggcgag ccgaatgagc
     1381 cccggcacgt tcacatgcac cgcgtggccc gcgacgtgaa cgtgggcgat ccacgccgga
     1441 gccgcggtcg acaacacgcc gttggagagc accccgacgc tgatcgcgcc agcgaggcag
     1501 atcgcagcga gcccgagggt gagtccaatc cagaacagcg cccgcgaaag ccatctcatc
     1561 ggggctctcc gtcaggctcg accactgtcg tggcccacca gggtccgccg ccgtattgcg
     1621 cggcccagaa ccaggccgag gtatcggtcc aggcgttacc tgcactgtca tggatgcgtt
     1681 cgcagacgcg cagtcgcgcg ccatcgcgcg cagc
//
LOCUS       DATIRT010000307         3597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_932383, whole genome shotgun sequence.
ACCESSION   DATIRT010000307 DATIRT010000000
VERSION     DATIRT010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932383"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1787)
                     /locus_tag="VLN25_06995"
     CDS             complement(<1..1787)
                     /locus_tag="VLN25_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSQ10344.1"
                     /translation="MKSFNLTEWALGHRAVVLFLILVIGIGGVLGFSKLGQLEDPNFS
                     VPSMTAMVIWPGATAQQVQDELLNRMEKKFEQLDHFEKVKTYARQGYGAMLITVAGGT
                     SHADQQEAWYQARKKFSDIKNELPEGVIGPIFNDEFGDVTGLLYAVKGDGVSLAELSD
                     VSEDIKRRLLKVPMVKKIDIVGKQAKKVYVEFSHQRLAALGITPLAIAESLRNQNSVL
                     SSGSIDTNSDRVLVRVSGQVANLDDIRNVPIAAGGRLIRLGDFTTITRGYEDPPTYTV
                     RHNGQQVLMLGITMTNDGNIVDLGKAIENSVAKLQAELPYGVELELVADQPTVVSESI
                     WEFERSLLEALAIVLAVCLLSLGWRTGIVVGLSVPIVLGVVALVMLAMGWNLERVSLG
                     SLIIALGLLVDDGIIAVEMMVVKMESGWDRLKAAAFSYTATAMPRLTGALITVAAFMP
                     IGFSQSTTGEYAGGIFWIVGTAVLFSWVVSGIITPYLAVLMLPKDFGKHHHGGDPYDT
                     PFYRRLRGWIDLAIERRWWVIGATVAALAVSIFASRLVPQQFFPNSSRPELVVELRLK
                     EGSSFAATTEQVKKMEAVLAKDEDVRFFTA"
     gene            complement(1784..2899)
                     /locus_tag="VLN25_07000"
     CDS             complement(1784..2899)
                     /locus_tag="VLN25_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSQ10345.1"
                     /translation="MKKPLAIALLLIAPTAAMISGCSEKPAPEPLRSVRTAEIRYDKA
                     QETNRYVGTVQSRHEVNEAFRVGGKVVQRKVDVGQKVREGDVLAVLDDTDYRQAEEAA
                     RQHHVAAVALARQAESDRKRLEALKHDGSVSVSEDEKAQTGHETASASAEAADRQLEL
                     ARNRLKYTVLRASRSGVVTAVRAEAGQVVGEGQPIVSIASEGEPEIVADVPEDHLAVF
                     KASRYKAYLQSAPEQAFDVVLRELSPQAAAATRTFRARLKPATPRPLPLGATATLVIE
                     RPAGESPAAAIPAAALTQDKGKPAVWVVRRSGSEPAGTVNLQGVTVHGYRNDEVLVSG
                     APAGELVVTAGVQKMAPGLKVALPESSHGIAPKQAAR"
     gene            complement(2926..>3597)
                     /locus_tag="VLN25_07005"
     CDS             complement(2926..>3597)
                     /locus_tag="VLN25_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSQ10346.1"
                     /translation="IMPSGGEPVAPPARAGQPAALLERRPDLLAARAQLDAANARRQQ
                     AHAEWFPRLFLGALFGRGSADINDFSLGAARYTNAAALLAMPIFNAGRTQAINDIAEA
                     GQSESVLRYEDAIVRALEDVENTLATVRNQRQRADTLAAAAASAEAAFGRAQSLYSRG
                     QIDLLPLLDAQRQRLLVRVSSNDSHTQLLLDSVQLYKALGGGWQVFDPITKPTQAIAA
                     PTPNS"
BASE COUNT          561 a         1172 c         1217 g          647 t
ORIGIN      
        1 gcggtgaaga aacgaacgtc ctcgtccttc gccagcaccg cctccatttt cttgacttgc
       61 tcggtcgtgg cggcgaatga cgagccttcc ttcagtcgca gttcgacaac cagttcgggc
      121 cgcgagctgt tggggaagaa ctgctgcggt accaatcggc tggcgaagat cgagacagcc
      181 agggcggcga cggtcgcgcc gatgacccac cagcgccgct ctatggcgag gtcgatccag
      241 ccgcgcagcc tgcggtagaa cggcgtgtcg tacgggtcgc cgccatgatg gtgcttgccg
      301 aagtccttgg gcagcatgag tacggcgagg tagggcgtga tgatccccga aacgacccac
      361 gagaacagca ccgccgtgcc gacgatccag aagatgccgc ccgcgtactc gccggtggtc
      421 gattgggaga agccgatcgg catgaaggca gcaacggtga tcaacgcgcc ggtcagccgg
      481 ggcatggcag tcgccgtata ggagaaggcg gcggccttca gtcggtccca ccccgactcc
      541 atcttcacca ccatcatctc gaccgcgatg atgccgtcgt ccaccagcag gccgagcgcg
      601 atgatcagcg agccgagcga gacacgctca aggttccagc ccatcgccag catcacgagg
      661 gcaacgacgc cgagcacgat cggcaccgaa aggccgacca cgatgccggt gcgccagccg
      721 aggctgagca ggcagaccgc cagcacgatg gcgagcgcct cgagcagcga gcgttcgaat
      781 tcccagatgg attcactgac gacggtcggc tgatcggcga cgagctcaag ttcgacgccg
      841 tagggcagct cggcctgcag cttggcgacc gagttctcga tcgctttgcc gaggtcgacg
      901 atgttgccgt cgttggtcat cgtgatgccg agcatcagga cctgctgccc gttgtggcgc
      961 accgtgtagg tcggcggatc ttcgtagccg cgcgtgatgg tcgtgaagtc gccgagcctg
     1021 atcaggcggc cgccggcggc gatcggcacg ttgcggatat cgtcgaggtt cgcgacctgg
     1081 ccgctgacgc ggaccagcac ccggtcgcta ttggtgtcga tcgagccgct cgacagcacg
     1141 ctgttctggt tcctcaggct ctccgcgatg gccagtggcg tgatgccgag ggcggccagc
     1201 cgctggtgcg agaactcgac gtagaccttc ttcgcctgct tgccgacgat gtcgatcttc
     1261 ttgaccatcg gcaccttcag caggcggcgt ttgatgtctt cggatacgtc ggaaagttcc
     1321 gcgaggctga cgccgtcgcc cttgacggca tacaacagcc cggtcacgtc tccgaattcg
     1381 tcgttgaaaa tcgggccgat gacgccttcg ggcagttcgt tcttgatgtc gctgaacttc
     1441 ttgcgcgcct gataccaggc ctcctgctga tccgcgtgcg atgtgccgcc ggccaccgtg
     1501 atcagcatgg cgccgtagcc ctggcgcgcg taggtcttga ccttctcgaa gtggtcgagc
     1561 tgctcgaatt tcttctccat ccggttgagc agctcgtcct ggacctgctg cgccgtggcg
     1621 ccgggccaga tcaccatcgc cgtcattgac ggcaccgaga agttcgggtc ttcgagctgg
     1681 ccgagcttcg agaagccgag cacgccgccg atgccgatca ccaggatcag gaagagcacc
     1741 acggcgcggt ggccgagtgc ccattcggtg aggttgaatg acttcatcgg gcagcctgct
     1801 tcggcgcgat gccgtgggag gattcgggga gcgcgacctt gagtcccggc gccatcttct
     1861 gcacgccggc ggtcacgacc agttcgccgg ccggggcacc ggacacgagt acctcgtcgt
     1921 tgcgatagcc atgcacggtc acgccttgca ggttcaccgt cccggcaggc tctgaaccgc
     1981 tgcggcgcac tacccacacg gcgggcttgc ccttgtcctg cgtgagtgcg gcggccggga
     2041 tagccgcggc ggggctttct cccgcagggc gttcaatcac gagcgttgcc gtcgctccga
     2101 gcggcaacgg ccggggagtc gcgggcttca agcgggcgcg gaaggttcgg gtcgccgccg
     2161 ccgcctgcgg cgaaagctcg cgcagtacta cgtcgaacgc ctgctccggc gcgctctgga
     2221 ggtacgcctt gtacctcgac gccttgaaaa cggccaggtg atcctccggc acgtcggcga
     2281 cgatttcggg ctcgccttcg ctggcgatcg agacgatcgg ctggccttcg ccaacgacct
     2341 gccccgcctc ggctcgcacg gccgtgacga ccccactgcg cgacgcgcgc agcaccgtgt
     2401 acttgagccg gttgcgcgcc agctcgagct gccggtcagc ggcctcggcg cttgccgatg
     2461 ccgtttcatg gcctgtctgc gccttctcgt cttcggacac gctgaccgag ccatcgtgct
     2521 tcagcgcttc gagccgcttg cggtccgact cggcctggcg cgccaacgcc accgcggcga
     2581 cgtggtgctg ccgggccgct tcttcggcct ggcggtagtc agtgtcgtca agcaccgcca
     2641 gcacgtcgcc ctcgcggacc ttctggccga cgtcgacctt gcgctggacg accttgccgc
     2701 cgacgcggaa ggcctcgttc acttcgtggc gcgactgcac ggtgccaacg tagcggttcg
     2761 tctcctgtgc tttgtcgtaa cggatctcgg ccgtgcgaac gctgcgcaac ggctcgggcg
     2821 ccggtttctc ggagcagccg gagatcatcg cggcggtcgg cgcaatgagg agcagggcaa
     2881 tggcaagcgg tttcttcatg gaggttctcg ggcggtcggc gcggttcatg aattcggggt
     2941 cggtgcagcg atagcctgcg tgggtttcgt gatcgggtcg aacacctgcc agccgccgcc
     3001 gagcgccttg tagagctgta cgctgtcgag cagcagctgc gtgtgactgt cgttggagct
     3061 gacacggaca agcagccgct ggcgctgcgc gtcgagcagc ggcagaaggt cgatttgtcc
     3121 gcggctgtag agtgactgtg cgcggccgaa ggccgcttcg gccgatgcgg cagcagcggc
     3181 cagagtatcg gcgcgttgac gctggttacg gaccgtggcc agcgtgtttt cgacgtcttc
     3241 gagcgctcgc acgatggcgt cttcgtaacg caggacggat tcggactgac ccgcctccgc
     3301 gatgtcgttg atcgcctgcg tccgtccggc gttgaagatc ggcatcgcca gcagagccgc
     3361 ggcgttcgta tagcgcgcgg cgcccaggct gaagtcgttg atgtcggcgc tgccgcgccc
     3421 gaacaacgcg ccgagaaaca gacgcggaaa ccactccgca tgggcctgtt gccggcgcgc
     3481 gttggcggcg tcgagttgcg ccctggcagc gagcagatcg ggtctgcgct cgagcagcgc
     3541 ggcgggctgg cccgctcgcg ccggcggagc gaccggctcg ccgcccgacg gcatgat
//
LOCUS       DATIRT010000308         4544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1817751, whole genome shotgun sequence.
ACCESSION   DATIRT010000308 DATIRT010000000
VERSION     DATIRT010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1817751"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VLN25_07010"
     CDS             complement(<1..471)
                     /locus_tag="VLN25_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSQ10347.1"
                     /translation="MAQYVYTMIRVGKIVPPKREILKNISLSFFPGAKIGVLGLNGSG
                     KSTLLKIMAGLDTDIEGEAVPMPGLSIGFLPQEPQLDPAKTVKDEVEAGLGEVMQAKQ
                     KLEEIYAAYAEPDADFDKLSAEQAKFEAIIATSGADVEHQMEIAADALRLPPWEA"
     gene            597..1166
                     /locus_tag="VLN25_07015"
     CDS             597..1166
                     /locus_tag="VLN25_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HSQ10348.1"
                     /translation="MTAFLISIGVVALAEIGDKTQLLALALAAKYRKPWPIIFGILLA
                     TLANHLLAGAVGTWITRSIGPEPLRWALGLSFIAMALWMLVPDKLDDESEKASRFGIF
                     GTTLIAFFIVEMGDKTQIATVALAARYDALVAVVAGTTIGMMIANVPAVLLGNVAAEN
                     LPKRLMNGLAAVVFALIGIAVLWGADIGL"
     gene            1351..2886
                     /locus_tag="VLN25_07020"
     CDS             1351..2886
                     /locus_tag="VLN25_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008351412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSQ10349.1"
                     /translation="MNSSFEALGLSEALVRAVTDLGYTQPTPIQLQAIPPVLAGSDLM
                     AGAQTGTGKTAAFVLPILQRLSAAAPTAPREHRAPRVLILAPTRELAAQVEESVRDYG
                     KHVHAKCAVIFGGVSIRPQFDALKRGVDIVVATPGRLLDHVGQKTIDLSKIEVLVLDE
                     ADRMLDMGFLPDMKRVFAQLPKQRQTLLFSATFSDEIRTLAATLMRNPVSVQATPRNS
                     TVEAVDQKAISIERTRKSELLAHLIKDNQWFQVLVFTRTKHGANRLAEYLCKHGATAL
                     AIHGNKSQNARTRALADFKRGDLQALVATDIAARGLDIEQLPHVVNFDMPNVPEDYVH
                     RIGRTGRAGATGEAISLVSRDEEALLRDIERLIKRPIPRMTVPSYQPSAAVGATHDVE
                     QRPARAPHGRKSSSSRTTPRSPARREQPARSAPSHGKGRVSPPAQPGPSSGQRPNTSA
                     AKRSTPATGGARTTPSERSTAKPSTSAARGQTTRTQGTSGGDSLVRTNIPAFMQSPHR
                     RGR"
     gene            2929..3222
                     /locus_tag="VLN25_07025"
     CDS             2929..3222
                     /locus_tag="VLN25_07025"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSQ10350.1"
                     /translation="MDEPKIAIQHNPAAMRFEAVVDGMLCRSDYRLHGETMMLVHTEV
                     PAQLEGKGIASALVKAAFKHAADKGMDVLPVCSYVRAWVQRHPEVRPLLSDSK"
     gene            complement(3278..>4544)
                     /locus_tag="VLN25_07030"
     CDS             complement(3278..>4544)
                     /locus_tag="VLN25_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10351.1"
                     /translation="DPLRQLGAPVVVDATPTVGVNTRMEIVLVPVASGLFVPTMPTVD
                     AVRDEITRRFPIPRANITVTVRPSYTLTSTTDGLDTSSEWTNALIELNQLRSMEAGSN
                     ATRFYFGVVRRSGGGIAGIGYVPGRTALGWDSSAQWARTMSHELGHNLSRPHAPCGGV
                     ASPDPNYPYAGGVLSGTPLMDSVPAAIDIVSPVSQTDIMGYCSGSWFSYYNFREMQRY
                     MEGQPSLIATQIAADAVEQDMLLVSGIVGLDGLQLAPVQAMRAVPATGRGDYTLRLQL
                     RDGRTVEHAFDAELVDHAELPERHFAIAIPDPGAALARIDVLRGSTPVPVRSSGLATA
                     QRASRASIERLRGVDWNESNGKLRVEWDTAAASHVAVTYVLDGTRTVLGVSRTGGVAE
                     FDTTALPAGGRFDIALTDGLNARSLQVRR"
BASE COUNT          796 a         1532 c         1453 g          763 t
ORIGIN      
        1 cgcttcccac ggcggcaggc gcagcgcatc ggccgcaatt tccatctggt gttcgacgtc
       61 cgcgcccgag gttgcgatga tcgcctcgaa cttcgcctgc tccgccgaga gcttgtcgaa
      121 atccgcatca ggctcggcat acgccgcgta gatttcctcg agcttctgct tggcctgcat
      181 cacttcaccc aggccggctt ccacttcgtc cttgacggtt ttagccgggt cgagctgggg
      241 ctcctgcggc aggaagccga tcgaaagccc cggcatcggc accgcctcgc cctcgatgtc
      301 ggtgtcgagc ccggccatga tcttcagcaa cgtcgatttg ccggaaccat tgagcccgag
      361 cacgccgatc ttggcgccgg ggaaaaagct gagggaaatg ttcttcagga tctcgcgctt
      421 gggcggaacg atcttgccca cgcggatcat cgtgtaaacg tactgcgcca ttggagttcc
      481 gtgccgagtg ctgcgaaatg tggagcgcga ttatcgcagc atgatcgcgg gcaaggcagt
      541 ccagtacttc acgaacctac aatgcgcggc agcgtgcagc gtggccacct ccagacatga
      601 ccgcgttcct gatatccatc ggcgtcgttg ccctcgccga gatcggcgac aagacgcagt
      661 tgcttgcgct tgcactggcc gcgaagtacc gcaagccgtg gccgatcatc tttggcatcc
      721 tgcttgccac gctcgcgaat cacctcctcg ccggcgcggt cggcacctgg atcacgcgct
      781 cgatcggccc agagccactg cggtgggcgc tcgggctttc gttcatcgcg atggcgctct
      841 ggatgctggt ccccgacaag ctcgatgacg agtccgagaa ggcgtctcgt ttcggcatct
      901 tcggtacgac gctgattgcg ttcttcatcg tcgaaatggg cgacaagacg cagattgcga
      961 ccgtggcgct ggccgcgcgc tatgacgcgc tggtggccgt cgttgcggga acgacgatcg
     1021 gcatgatgat cgcgaacgtg cccgccgtgc tgctgggcaa cgtcgccgcc gagaatctgc
     1081 ccaagcgact gatgaacggg ttagccgcgg tcgtgttcgc gctgatcggc atcgcggtgc
     1141 tgtggggcgc ggacattggc ctgtgaggct aaacccgcct aagtcattga cttcgtgtac
     1201 cattcgcccc tgattgcggt gcgtcggttc cggttcgcac ccgtccatcg tggttgtcaa
     1261 cacgctccct cgaagtctat ttccctagtt gcatctgcct gaaccggtcc ggcgagcaaa
     1321 gttcgcgcag gccgtgctca ggagtttcgt ttgaattcat cgtttgaagc cctcggcttg
     1381 tccgaggcac tcgtacgcgc ggtcaccgac ctcgggtaca cccagcccac gccgatccag
     1441 ttgcaggcga ttcccccggt ccttgcgggc agcgacctga tggcaggcgc gcagaccggc
     1501 acgggcaaga ccgccgcgtt tgtgctgccg attctgcagc ggctaagcgc cgcagcgcca
     1561 accgcaccgc gcgaacaccg cgcaccgcgc gttctgatcc ttgcgccgac gcgcgaactc
     1621 gccgcgcagg tcgaagaaag cgtgcgcgac tatggcaagc acgtgcacgc caagtgcgcc
     1681 gtcatctttg gtggcgtgtc gatccggccg cagttcgacg cgctcaagcg cggcgtcgac
     1741 atcgttgtgg ccacgcccgg ccgactgctc gatcacgtcg gccagaaaac gatcgacctg
     1801 tcgaagatcg aagtgctggt cctcgacgaa gccgaccgca tgctcgacat gggcttcctg
     1861 cccgacatga agcgagtttt tgcgcagttg ccgaagcagc ggcagacgct gctcttttcg
     1921 gccacgttct cggacgagat ccggacgctg gcggccacgc tgatgcgcaa cccggtgtcg
     1981 gtccaggcca cgccgcgcaa ctcgaccgtc gaggccgtcg atcagaaggc gatcagcatc
     2041 gaacgcaccc gcaaatccga gctgcttgcg cacctgatca aggacaacca gtggttccag
     2101 gtgctggtat tcacgcgcac caagcatggc gccaatcgcc tggccgagta tctctgcaag
     2161 cacggtgcca cggccctcgc catccacggc aacaagagcc agaatgcgcg cacgcgtgcg
     2221 ctggcggact tcaagcgcgg ggacctgcag gcgctggtcg cgaccgacat cgcggcgcgc
     2281 ggactcgaca tcgaacagct gccgcatgtc gtcaacttcg acatgccgaa cgttccggag
     2341 gactacgtgc accgtatcgg ccgcacgggc cgcgccggcg cgaccggaga ggcgatctcg
     2401 ctcgtgagcc gcgacgaaga agcgctgctg cgcgatatcg agcgcctgat caagcgcccg
     2461 atcccgcgca tgaccgtgcc cagctatcaa ccgtcggccg cggtcggcgc aacgcacgac
     2521 gtcgagcagc gaccggctcg cgcaccgcac ggccgaaagt cgagttcctc gcgcacgaca
     2581 ccccgctccc cggcacgacg cgagcagccg gcgcggtccg cgccgtcgca cggcaagggg
     2641 cgcgtcagtc caccggcaca acccggtcca tcgtccggtc agcggcccaa caccagcgcg
     2701 gcgaagcgct cgacgccggc gaccggcggc gcacgcacga cacccagtga acgttcgacg
     2761 gcgaagccct ctacgtcagc cgcgcggggc cagacgaccc gcacgcaggg aacgtccggc
     2821 ggggactcgc tggtccgcac caacatacct gcgttcatgc agtcgccgca tcgccgcggg
     2881 cgctaagctt ccgtctctga cgggggagaa ccgcgacgcg aggtctcgat ggacgaaccg
     2941 aaaatcgcga tccagcacaa tccggccgcg atgcgctttg aggcggtggt ggacggcatg
     3001 ctgtgccggt ccgactatcg cttgcacggc gagacgatga tgctggtgca taccgaggtg
     3061 ccggcgcagc tggagggcaa gggcatcgcc tcggccctcg tgaaggccgc gttcaaacat
     3121 gcggccgaca aaggcatgga cgtgctgccc gtgtgctcgt atgtgagagc gtgggtgcag
     3181 cgccatccgg aagtcaggcc gctgctgtcc gacagcaagt gactttcgac cgcgtcgcaa
     3241 tggcctgcgg cgcgctttgc acggcagcgc gctaacgcta gcggcggacc tgcagcgagc
     3301 gggcgttcag cccatcagtg agcgcgatgt caaatcgacc accggccggc agagcggtgg
     3361 tgtcgaattc ggccacgcca cccgtgcgac tcacaccgag cactgtgcgg gtgccgtcga
     3421 gcacataggt gaccgcaaca tgcgacgccg ccgccgtatc ccactcgacg cgcagcttgc
     3481 cgttgctttc gttccagtcg acaccacgca accgctcgat gctcgcccgg ctggcgcgtt
     3541 gcgccgtggc caggccggag gatcgcaccg gaaccggagt cgagccgcgc agaacgtcga
     3601 tgcgcgcgag cgcggcaccc gggtccggga tcgcaatcgc aaagtgacgc tcgggcaact
     3661 cggcgtgatc gaccagctcg gcatcgaatg cgtgttcgac cgtgcgaccg tctcgcaact
     3721 gcagccgcag cgtgtagtcg cctctaccgg tcgcaggcac ggcacgcatg gcctgaaccg
     3781 gcgcgagctg tagcccgtcc agcccgacga tgccggagac cagcagcatg tcctgctcca
     3841 ccgcgtcggc ggcgatctga gtagcgataa ggctcggctg cccttccatg tagcgctgca
     3901 tttcacggaa gttgtagtag gagaaccacg aaccgctgca gtaccccatg atgtcggtct
     3961 ggctgacggg cgacacgatg tcgatcgcgg caggcaccga atccatcagc ggggtgccac
     4021 tcagcacgcc tccggcatat gggtagttgg gatccggact cgccacgccg ccacacggcg
     4081 catgcggccg gctgaggttg tgtcccagtt catgcgacat cgttcgcgcc cactgcgcac
     4141 tcgagtccca gccgagcgcg gtacgccccg gcacatagcc gatgccggca atcccgccgc
     4201 cggaccggcg cacgacgccg aagtagaagc gggtcgcgtt cgaacctgct tccatcgagc
     4261 gcagctggtt gagttcgatc agggcattcg tccactcgct cgacgtgtcg aggccatcgg
     4321 tcgtactggt cagcgtgtac gacgggcgca ctgtgacggt gatgttggcg cgcgggatcg
     4381 gaaagcgacg cgtgatctcg tcgcgcaccg catcgacggt cggcatcgtc ggcacgaaca
     4441 atcccgaggc caccggcacc agcacgatct ccatgcgggt gttcacaccc actgtgggcg
     4501 tcgcgtcgac gacgacgggc gctccgagtt gccgcaaggg atcg
//
LOCUS       DATIRT010000309         6203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1227924, whole genome shotgun sequence.
ACCESSION   DATIRT010000309 DATIRT010000000
VERSION     DATIRT010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6203
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227924"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="VLN25_07035"
     CDS             <1..958
                     /locus_tag="VLN25_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10352.1"
                     /translation="KLVSPKVEKGSPPTPGDDTVVEHEYERPKGKMYLYWGCGESVRA
                     GQPRVLDMATARPDEYGKFFTSRRATQRGAHSAAGRPLWPNEVDSRMVPGSASLVGEH
                     AFTGQGVPEGFKFAIGSQQDLMPAIELKQGDAGGATPLEWAALPTARAYFIAAMGAKG
                     GSEMEMVFWTSSEVPELGMGLIDYQTNPSVDRWLKEKVLLEPSVTRCTVPKGIFGEGG
                     GAMLRMIAYGSELNLAHPPRPTDPKIAWEPQWAVKLRVKSVANAFLGMDMGGMQRGSG
                     RQAQPQGQPQGQSQGTAPPAAEKKEEDKLPDPVNILRGIFGR"
     gene            1105..1353
                     /locus_tag="VLN25_07040"
     CDS             1105..1353
                     /locus_tag="VLN25_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3297 family protein"
                     /protein_id="HSQ10353.1"
                     /translation="MDEPSSVPPLPDRLSVNPRSPFHAAAVLEHDIGVRFNERERSDV
                     EEYCISEGWVKVPAGKTLDRKGQPLLITLKGKVEVFYR"
     gene            1434..3554
                     /locus_tag="VLN25_07045"
     CDS             1434..3554
                     /locus_tag="VLN25_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HSQ10354.1"
                     /translation="MRILRRLFLLLALASSCAAVLAQASASSGADDPYLWLEDVGGDT
                     ALGWVRSRNAVAEKEFAADPRYEPLRAHLLTILNSQERIPYVTRKGEHVYNFWRDAAN
                     PRGLWRRTTLADYRKAQPAWEVVIDLDAVAAADKENWVWKGAECLRPEKPGEPYRRCL
                     VALSRGGADATVVREFDLQDMRFVGDGFNLPVEAKQDLKWKDIDTVWVATDFGPGSMT
                     TSGYPRIVKEWKRGTLLATARTVFEGKESDVGVDASSERESGRRRDWVGRYIEFWNTE
                     HYVERDGTLVRVEVPADATITPFKDWLLVRTRTPWTVGGKAWPAGALLAVRFEEFMKG
                     ERTFDLLYAPSARSSIEDVEVTRTAVLVSELDNVRPRLWELQHDGKQWQRRRVTLPEQ
                     GQIGQLTTYWDSDDYLLTHQDFTTPTTLLERRVGSAEAASLKSLPRFFDGSGIVTRQY
                     EAISKDGTRIPYFIAMRESLKLDGRSPTLLYGYGGFQNAQLPWYSGVFGKSWLEPGGV
                     LALANLRGGGEFGPDWHRAAQRENKQRTWDDMAAVAEDLIRRGITSPPHLGIMGGSQG
                     GLLVTATMVQRPELFGAVVSQVPLIDMLRYHKLLAGASWMAEYGDPDQPADRAFIAKY
                     SPYQNVIKGAKYPRIFIVTSTRDDRVHPGHARKMTARMLEQGHDVLYFENIEGGHGAA
                     ANNAQAARMWAQTFSFLWRQLGSH"
     gene            complement(3652..4284)
                     /locus_tag="VLN25_07050"
     CDS             complement(3652..4284)
                     /locus_tag="VLN25_07050"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSQ10355.1"
                     /translation="MPLTPEELQLAARRTLEHYDQRAEAFWEGTRDHDVSQNISALLQ
                     YIEAEPPFELLDFGCGPGRDLKTFKALGHHATGLEGSAPLAALARAHGGCDVLEQNFL
                     ALDLPRDHFDGVFANAALFHIPSQALPAVLRELHKALKPNGVLFSSNPRGDGQEGWNG
                     DRYGVFHDWENWRGYMTAAGFTELTHYYRPVGLPREQQPWLASVWRKPVT"
     gene            4449..4682
                     /locus_tag="VLN25_07055"
     CDS             4449..4682
                     /locus_tag="VLN25_07055"
                     /inference="COORDINATES: protein motif:HMM:NF042781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6719 family protein"
                     /protein_id="HSQ10356.1"
                     /translation="MAVLLGVMSGVLVAIAAQAGQTVLKEVPKEGDIPYGKVVLVDDG
                     TCPKGEVKEITGGSSAKAIPRKTRCVKRPARTE"
     gene            complement(4825..5031)
                     /locus_tag="VLN25_07060"
     CDS             complement(4825..5031)
                     /locus_tag="VLN25_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10357.1"
                     /translation="MKRKHEAKNDFFYAFVMFAMFAVVVFNVAAEFFHRYPEAFFVDF
                     NEAVEPRVMAADADAHAVVAMRAA"
     gene            5458..>6203
                     /locus_tag="VLN25_07065"
     CDS             5458..>6203
                     /locus_tag="VLN25_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10358.1"
                     /translation="MSTPVDAFLNTAWADHGEHPQDVAERLSESIGLLQAREQFAPYV
                     RLVTHVYGEHLGQWHGAVELLNRLRTLPASAASPDVTTTLDQAVATMRFAAGEAAALD
                     PLPLEARLSALAMAASALAAREELLRAINALECALQLAKGGLPEGSSANRALAVAGNN
                     LAAVLEGKPERDARQTAAMLVAAEAGLTYWKLAGTWLEEERAEYRLARSQLQAGQARD
                     AAASAQRCIAVCRRNGAPAFEQFFGFAVLAL"
BASE COUNT         1146 a         1919 c         2094 g         1044 t
ORIGIN      
        1 gaagctggtc tcgccgaagg tcgagaaggg atcgccgccg actccgggcg acgacaccgt
       61 ggtcgaacac gagtacgagc gtccgaaggg caagatgtac ctgtactggg gctgcgggga
      121 gtcggtgcgg gcaggacagc cgcgcgtgct cgacatggcg accgcgcgcc cggacgagta
      181 cggcaaattc ttcacctcgc gccgcgcgac ccagcggggc gcgcacagcg cggccggccg
      241 accgctgtgg ccgaacgaag tcgactcgcg catggtgccg ggcagtgcgt cgctggtcgg
      301 ggagcacgcg ttcaccggac agggcgtgcc tgaaggtttc aagtttgcga tcggctccca
      361 gcaggacctg atgccggcga tcgagctgaa gcagggcgat gcgggcggcg ccactccgct
      421 cgaatgggcc gcgctgccga ccgcgcgcgc ctacttcatc gccgcgatgg gggcgaaggg
      481 cggctcggaa atggagatgg tgttctggac atcgagcgaa gtgcccgagc tcggcatggg
      541 gctgatcgac taccagacca acccgtcggt cgatcgctgg ctcaaggaga aagtcctgct
      601 cgagccgagt gtcacgcgct gcaccgtgcc gaagggaatc tttggagagg gcggcggcgc
      661 aatgctgcgc atgatcgcgt acggctccga actcaatctc gcccatccgc cgcgcccgac
      721 cgatccgaag atcgcgtggg agccgcagtg ggccgtgaag ctacgcgtga agagtgtggc
      781 gaatgccttt ctcgggatgg acatgggcgg catgcagcgc ggctccggac gccaggcaca
      841 accgcagggc cagccacagg gccagtcaca aggcacggcg ccgcccgcgg ctgaaaagaa
      901 ggaagaagac aagttgcccg acccggtcaa catcttgcgc ggcatcttcg gacgctaaga
      961 atctgcacgc cggcgcgttc gtcctcgtca ttcgaggccg gacgcgcatg gcgtgcgcga
     1021 tggcgcttca acaaagccgc gtgacacgca cgaacagatc actggaaatc acaggaaatc
     1081 gcaggaagtc acaggaacac tccgatggac gaaccctcca gcgtgccgcc gctgcccgac
     1141 cgcctctcgg tcaacccgcg cagcccattc catgccgccg ccgtactcga gcacgatatc
     1201 ggcgttcgct tcaacgagag ggaacgttcg gatgtcgagg agtactgcat cagcgaaggt
     1261 tgggtgaagg tgcccgcggg caagacgctg gatcgcaagg gccagccgct gctgatcaca
     1321 ctgaagggta aggtcgaggt cttctatcgg tgatggcctc gatgcctgca atggcgccgc
     1381 tccgagcaag gactcgcagt gactcccgac gaatcggccc tggaaatttg aagatgcgta
     1441 ttttgcgacg actgttcctt ctgctcgcgc tggcaagttc ctgcgccgcc gtgcttgcgc
     1501 aggcctccgc ttcatcgggc gcggatgacc cctatctctg gctcgaagat gtgggcggcg
     1561 acacagcgct cggctgggtg cgctcgcgca acgccgtggc ggaaaaggag ttcgccgccg
     1621 atccgcgcta cgagccgttg cgcgcccacc tgctcactat cctcaactcg caggagcgga
     1681 tcccatacgt gactcgcaag ggcgagcacg tctacaactt ctggcgcgat gcggccaatc
     1741 cgcgcggact ctggcgccgc acgacgcttg ccgactaccg caaggctcag cccgcgtggg
     1801 aggtggtcat cgatctggac gccgtggcgg cggccgacaa ggagaactgg gtctggaaag
     1861 gggccgaatg cctgcgtcct gaaaagccgg gcgagcccta tcgccgctgc ctggtcgcac
     1921 tctcgcgcgg aggtgccgac gcgaccgtcg tgcgcgagtt cgacctgcag gacatgcgct
     1981 tcgtgggcga tggcttcaac ctgccggtcg aggccaagca ggatctcaaa tggaaggaca
     2041 tcgacacggt ctgggtcgcc accgacttcg gtcccggttc gatgaccacg tcgggctatc
     2101 cgcgcatcgt caaggaatgg aagcgcggca cgctgctggc gacggcgcga acggtctttg
     2161 agggcaagga atccgatgtc ggcgtcgacg ccagttcgga gcgcgagtcg ggtcggcggc
     2221 gagactgggt cggccgctat atcgagttct ggaataccga gcactacgtc gagcgcgacg
     2281 gcacattggt tcgggtcgag gtcccggccg acgccacgat cactccgttc aaggactggc
     2341 tgctggtgcg cacgcgcacg ccctggacgg tgggcggaaa ggcatggccg gccggcgcct
     2401 tgctggcggt ccgcttcgaa gagttcatga aaggagagcg cacattcgac ctgttgtatg
     2461 cgccgtccgc tcgcagttcg atcgaggatg tggaggtgac gcgcaccgca gtgctggtga
     2521 gtgaactcga caatgtgcgt cctcggctgt gggagctgca gcacgacggc aagcagtggc
     2581 agcgtcggcg agtgacgctg cccgaacaag ggcagatcgg ccagctgacc acgtactggg
     2641 acagcgacga ctatctgctg acgcatcagg atttcacgac gccgactacg ctcttggagc
     2701 ggcgcgtcgg tagtgcggaa gcggcctcac tgaagtcatt gccacgcttc ttcgatggca
     2761 gcgggatcgt cacgcggcag tacgaggcga tcagcaagga cggcacgcgc ataccgtact
     2821 tcatcgcgat gcgcgagagc cttaagctag acggacgcag tccgacattg ctctacggct
     2881 acggtggctt ccagaacgcg cagctgccgt ggtactcggg tgtgttcggc aaaagctggc
     2941 tcgagcccgg cggtgtgctg gcgctggcga acctgcgcgg cggaggcgag ttcgggcccg
     3001 actggcatcg cgccgcgcag cgcgagaaca agcagcgcac ctgggacgac atggccgctg
     3061 ttgccgaaga cctgatccgg cgtggcatca cctcgccacc tcaccttgga atcatgggcg
     3121 gcagccaggg cgggctgctg gttaccgcga cgatggtgca gcgaccggaa ctctttggtg
     3181 ccgtcgtttc gcaggtgccg ctgatcgaca tgctgcgtta tcacaagctg ctggccggcg
     3241 cctcgtggat ggccgagtac ggtgacccgg accagccggc cgatcgcgcg ttcatcgcga
     3301 agtactcgcc ctatcagaac gtgatcaaag gtgcgaaata cccgcgcatt ttcatcgtga
     3361 catccacgcg tgacgaccgc gtccatccgg gacacgcacg caagatgacg gcgcgcatgc
     3421 tggagcaagg gcacgacgtg ctgtacttcg agaacatcga aggggggcac ggcgcggccg
     3481 ccaataatgc gcaggccgcc cgcatgtggg cgcagacctt cagctttctc tggcggcaac
     3541 tcggttcgca ctaagaccgg ctaaaacccg ccaggacccg cggaaacccg cgaggtaggg
     3601 tcgacggtcg ggtgttcgac tcgtcaacgg cattcttcgt cgacccttcg ctcaggtgac
     3661 gggtttgcgc cacacgctgg ccaaccaggg ctgctgctcc cgcggcaggc cgacgggccg
     3721 gtagtagtga gtgagttcgg tgaatccggc cgcagtcatg tagccgcgcc agttctccca
     3781 gtcgtggaac acgccatagc ggtcgccatt ccatccctcc tggccgtcgc cgcgcggatt
     3841 cgaactgaag aggacaccgt ttggcttcag ggctttgtgc aactcgcgca ggacggcagg
     3901 cagagcctgg ctgggaatgt ggaacagtgc cgcgttggcg aatacgccgt cgaagtggtc
     3961 gcgtggcagg tcgagggcaa ggaaattctg ttcgagcacg tcgcatccgc cgtgggcacg
     4021 cgccagcgcc gccagcggtg ccgatccttc gaggccagtc gcgtgatgtc cgagcgcctt
     4081 gaacgtcttg aggtcgcgcc cgggcccgca tccgaagtcg agcaactcga acggaggctc
     4141 cgcctcgatg tactgcagca acgcgctgat gttctggctc acgtcgtgat cgcgcgtgcc
     4201 ttcccagaac gcctccgcac gttggtcgta gtgctccagc gtccgacggg cggcgagttg
     4261 aagctcttca ggcgtgagtg gcatcttcgc taggccggcg tcagtggggc tggaacgaat
     4321 agggtcgggg tcagcgtttg gcgaaggccg acgtctgcga acgatagtct ggtgcaagct
     4381 ggcgtagcca agcgtcgggc gttcatctac aaacacaaac gggaccttgc ctccctggag
     4441 tcacgcaaat ggccgtgttg ctgggtgtca tgtcgggtgt gctggtggcc attgcggccc
     4501 aggccggcca aaccgtattg aaggaagttc cgaaagaggg cgacattccg tacggaaagg
     4561 ttgtgctcgt ggacgacggc acctgcccca agggcgaggt gaaggaaatc acgggtggca
     4621 gcagtgcaaa ggcgataccg cgcaagacgc ggtgcgtgaa gcgaccggcc agaaccgagt
     4681 aggccacgag acgagtcgca atatccggat ttcggcggca gtcgtcccgt gcggcccgaa
     4741 gccgacgtca attgcagtcc gtagtccgaa cggctgtgca gtcatcgcaa agccggtcgg
     4801 aactgcaggt ttcattctcg actcctatgc cgcgcgcatt gccacaacgg catgcgcgtc
     4861 ggcgtccgcg gccatcacgc gaggctcaac ggcctcgttg aagtccacga aaaatgcctc
     4921 gggatagcga tggaaaaact ccgcggcgac attgaagacc accaccgcga acatcgcgaa
     4981 catcacgaag gcatagaaga agtcgttctt cgcctcatgc ttgcgtttca tgacactctc
     5041 ctgacgggtg gctgcagcca acaaaaagcc cggaggcgtt cgacgcgatc cgggctctgg
     5101 ttgcagcctg gtccagctaa atgcctggac ctccgcccgg atcatgggcc tgcatggaaa
     5161 ggcttggccg cgcgccggcc agcgacgcgg tctcgacagg cattccagcc tgccgacacg
     5221 tgtccacgaa agcgaactgg ggtcgcttcc tggacttgag tgtgccgatc gcgtcgctca
     5281 tctcggaact ttcaccttta ccggcgaaat tgccggtgtc gaaatgctac ggccggccgc
     5341 gctgccaatc aatcacattt gtgcgtgaat attctgagtc tgccgcccga ccattgcggc
     5401 ggactggcgc cggcggcaga attcccgggc aacgcatgtt tctgacgccg aacatccatg
     5461 agcacccctg ttgacgcgtt tctgaatacc gcttgggccg accatggcga gcacccgcag
     5521 gacgtcgccg aacgcctgtc ggagtcgatc ggattgcttc aggcacgcga gcagttcgcg
     5581 ccgtacgtgc ggctcgtgac gcatgtctat ggagagcacc tgggccagtg gcacggcgcg
     5641 gttgaactgc tgaatcggct tcgcacgttg cccgcgtctg ccgcaagccc ggatgtaacg
     5701 acgacgcttg accaggcggt ggcgacgatg cgatttgccg ccggcgaggc ggccgcgctg
     5761 gatcctctgc ctctcgaagc tcggctgtca gccctggcca tggccgcgtc ggcccttgcg
     5821 gctcgcgagg aactgctgcg agcgatcaac gcgctcgagt gcgcactgca gctggccaaa
     5881 ggcggattgc cggagggctc gtcggcgaat cgcgcactgg ctgtggcggg taacaacctg
     5941 gccgccgtgc tggaaggcaa gcccgagcgc gatgccaggc aaaccgcggc gatgctggtg
     6001 gccgcagagg ccggcctgac gtactggaaa ctggccggca cgtggctcga agaagaacga
     6061 gcggagtacc ggcttgcgcg cagtcagctg caggcggggc aggcgcgcga cgccgcggcc
     6121 agcgcccagc gctgcattgc ggtctgccgc aggaacgggg ctcctgcgtt cgagcagttc
     6181 ttcggctttg ccgttcttgc cct
//
LOCUS       DATIRT010000310         5095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1325894, whole genome shotgun sequence.
ACCESSION   DATIRT010000310 DATIRT010000000
VERSION     DATIRT010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325894"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VLN25_07070"
     CDS             <1..411
                     /locus_tag="VLN25_07070"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HSQ10359.1"
                     /translation="IATDGMPAMKMSGTESVRKLGDAWVLCEGSGSMGGGLALTQFTL
                     GYDPAKGQFVGTFIGSMMTHLWIYHRGELDAAGRTLALYAEGPAFDGPPGTMAQYRDS
                     IEMVGPDERLLLSHLKGPDGQWIQFMTTRYRRTR"
     gene            438..791
                     /locus_tag="VLN25_07075"
     CDS             438..791
                     /locus_tag="VLN25_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HSQ10360.1"
                     /translation="MSYMLLIVEPVGQRRERTEDEGRALYDSMLKFSADLKARGLLTV
                     SQSLRSDAEAVRVQVRQGKRTLIDGPFAEAKEMVGGYFLLTCKSREEAVAIAAECPAA
                     QWATVEVREFGPCFT"
     gene            850..2106
                     /locus_tag="VLN25_07080"
     CDS             850..2106
                     /locus_tag="VLN25_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HSQ10361.1"
                     /translation="MESARIIAALTRLVRDVGLAEELAQDALVAALEQWPVDGVPDNP
                     AAWLTQAAKHRAIDRIRRKALHERQQDAITWELETQLQDVAPDLEAQIEAAFDDRIGD
                     DLLRLIFIACHPVLSREARTALTLRLLGGLTTDEIARAFLVPEPTIAQRIVRAKRTLA
                     EARVPFELPPATELAARLASVLEVLYLIFNEGYAATAGDDWMRPALAEEALRLGRILA
                     GLAPRQSEVHGLVALMEIQASRLRARTGPDGAPILLLDQDRARWDPMLIRRGLDALDR
                     ADELARAQGGLGSYALQAAIASCHARAKTADATDWKRIAALYDALAQRAPSPVVELNR
                     AVALAMAFGPAAGLEVVDTLLNDAQLRDYHLLPSVRGDLLFKLDRFEEARNEFARAAS
                     MTRNAREQALLLRRAGECAQRAGPSR"
     gene            complement(2125..2715)
                     /locus_tag="VLN25_07085"
     CDS             complement(2125..2715)
                     /locus_tag="VLN25_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diheme cytochrome c-553"
                     /protein_id="HSQ10362.1"
                     /translation="MKRIPVEYAVLAIAVAATLAVTLPARAQTPKKSAETTADKVARG
                     KYLVTTSGCHDCHTPWIMGEKGPEPDMSRMLSGHPESEKLPPPPAAQGPWIMTASATN
                     TAWSGPWGVSYTANLTPDKETGLGKWTQRNFTETIRTGRHMGRGREILPPMPIQVYKN
                     FTDADLASIFSYLQSIPAVSNRVPEPLPSALPVAAK"
     gene            complement(2712..3782)
                     /locus_tag="VLN25_07090"
     CDS             complement(2712..3782)
                     /locus_tag="VLN25_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10363.1"
                     /translation="MKALHLLVSLIATAAVSAALAAQPAVPQLPRLLSETGLFKAGST
                     TVVRAEVIAFAPQYPLWSDGTRKQRWISLPRGRAINASQPDAWQFPIGTRLWKEFGYG
                     GRIETRMIERLPDGSWRFASYAWNADGTDAVLVPEGGAVVAAEGAPGGRYSVPSRNDC
                     LACHDGPAVPVLGFSALQLSSDRDPLAPHADAPRRDFADLRSLVALGRIVNLSPALLA
                     APVRIAARTPTERAALGYLHGNCGHCHSASGALDGLELMLSQSAGASVDSAEQTLRSL
                     LGHSSRFRPQGAATAQRVAADGSGAHMLTLRMKSENRMARMPPLGVQVVDTEGVALVE
                     RWITEAVQASREPQHLAKGELK"
     gene            complement(4010..4939)
                     /locus_tag="VLN25_07095"
     CDS             complement(4010..4939)
                     /locus_tag="VLN25_07095"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HSQ10364.1"
                     /translation="MHRQRDLPTSLDLLRGFEASARLLSFTAAATELFLTQSAISRQV
                     QQLEEQLGVKLFERRTRALVLTDAGDRYYRDVSKVLDQLREATAAVRVQATPVVRVTT
                     TLTFASLWLVPRLASFQKEHADISVHVVADNVVRDLERSSLDVALRYCPEEAAGPGAV
                     RLFGEEVAPVAAPSLLKGQRVRSVEDLLRLPLIELEDPNGIALWLSWKVWCEAMGVPR
                     PRTSRGITFSHYDQVVQAAVAGQGVALGRFPLLDPFLGERRLALPLKGRQYATLARRA
                     YWLLGAPGAAKRPEVSTFMTWLRKQVAVQRGPE"
BASE COUNT          844 a         1714 c         1727 g          810 t
ORIGIN      
        1 atcgccactg acggcatgcc ggcgatgaag atgagcggca ccgagagcgt gcgcaagctc
       61 ggcgacgcct gggtgctgtg cgaagggagc ggctcgatgg gcggcgggct cgcactcacg
      121 cagttcacgc tgggctacga cccggccaag gggcagttcg tcggcacctt catcggttcg
      181 atgatgacgc acctgtggat ctaccaccgc ggcgaactcg atgccgccgg ccgcacgctg
      241 gcgctgtacg ccgaagggcc ggcatttgac ggaccgccgg gaacgatggc gcagtatcgc
      301 gactcgattg aaatggtcgg gcccgatgag cggctgctgc tgagccacct gaagggcccc
      361 gacgggcagt ggatccagtt catgaccacg cgctatcggc gcacacgcta gcgcggcacg
      421 gccgcacgga gtcccgcatg agttacatgc tgctgatcgt cgagcccgta ggccagcgac
      481 gcgagcgcac cgaagacgaa ggccgcgcgc tgtacgacag catgctgaag ttcagcgcgg
      541 atctcaaggc gcgcggtctg ctgaccgtca gccagtcgct gcgcagcgat gccgaggcgg
      601 tacgcgtgca ggtgcggcaa ggcaaacgca cactgatcga cggtccgttt gccgaagcaa
      661 aggagatggt cggcggctac ttcctgctca cgtgcaagag cagggaggaa gcggtggcga
      721 tcgcggccga gtgccccgcc gctcagtggg ctaccgtcga agtgcgcgag ttcggcccct
      781 gcttcacctg atccgttgag tgattcgacc gcagatggtc tgcatcgcac gatcgatgcg
      841 gtgtggcgaa tggagtccgc acgcatcatt gccgctctga cgcggctggt gcgcgacgtc
      901 ggtctcgccg aggaacttgc acaggatgca ctcgtcgctg cgctcgaaca gtggcccgtc
      961 gacggcgtgc ctgacaaccc cgcggcctgg ctgacccagg cggcgaaaca ccgtgcgatc
     1021 gaccgcatcc gccgcaaggc actgcacgag cgccagcagg atgcgatcac ctgggaactc
     1081 gaaactcagc tgcaggatgt agcgcccgac ctcgaggcgc agatcgaagc ggcgttcgac
     1141 gaccggatcg gtgacgacct gctgcggctg atcttcatcg cgtgccatcc ggtgctgtcg
     1201 cgcgaggcgc gcacggcgct gacgctgcgc ctgctcggcg gtctcaccac ggacgagatc
     1261 gcacgcgcgt tcctcgtccc cgagccgacc atcgcacagc gcatcgtgcg cgcgaagcgg
     1321 acgctggccg aggcacgcgt gcccttcgag ctgccgcccg ccactgagct cgcggcacgc
     1381 ctggcctcgg tgctggaagt gctctacctc atcttcaacg agggttacgc cgcgaccgcg
     1441 ggcgatgact ggatgcgacc cgcacttgca gaggaggcgc tccggctcgg tcggatcctg
     1501 gccgggctgg cgccgcggca atccgaggtc cacggactgg ttgcgttgat ggagatccag
     1561 gcctcccgac tgcgcgcgcg cactggaccg gacggcgcgc cgatcctgct gctcgaccag
     1621 gaccgggccc gttgggaccc gatgctgatc cggcgcggac tcgatgccct cgatcgggcc
     1681 gacgaactcg cgcgtgctca gggcggactc ggctcctacg cgctgcaggc agcgattgcg
     1741 tcgtgccacg cccgcgcgaa gactgccgat gcaaccgact ggaaacgcat cgccgcgctg
     1801 tacgacgcac tggcgcagcg cgccccatcg cccgtcgtgg aactgaatcg tgccgttgcg
     1861 ctcgcgatgg ccttcggtcc ggcggcggga ctcgaagtgg tggacacgtt gctcaacgac
     1921 gcgcagctca gggactacca cctgctgccg agcgtgcgcg gtgacctgct gttcaagctg
     1981 gaccgctttg aggaggctcg caacgagttc gcgcgggcgg cgtcgatgac acgcaatgct
     2041 cgcgaacagg cgctactgct gcggcgtgcc ggcgagtgcg cgcagcgggc cgggccctcg
     2101 cgctgaggac gcggcccgcg cgtactactt ggccgcgacc ggcaacgccg acggcagcgg
     2161 ttccggtacg cggttactca ccgccgggat cgactgcagg tagctgaaga tcgatgcgag
     2221 gtcggcatcc gtgaagttct tgtacacctg gatcggcatc ggcggcagga tctctcggcc
     2281 acgccccatg tgacggcccg tgcggatggt ttcggtgaag ttgcgctgcg tccacttgcc
     2341 gaggcccgtt tccttgtcgg gagtcaggtt ggcggtgtag ctcacacccc acggcccgga
     2401 ccacgcggtg ttggtagcag aggctgtcat gatccacggc ccctgggccg ccggcggcgg
     2461 cggcagcttc tcgctctcgg gatgcccgga cagcatgcgg ctcatgtcgg gctcggggcc
     2521 tttttctccc atgatccacg gcgtgtggca atcgtggcag ccggatgtcg tgaccaggta
     2581 ctttccgcgc gccaccttgt cggcggttgt ttcggcggac ttcttgggcg tttgcgcgcg
     2641 ggccggcagg gtgacggcaa gcgtggccgc aaccgcaatc gcgaggacgg cgtattccac
     2701 ggggatgcgt ttcatttcaa ttctcctttt gccaggtgtt gaggttcgcg tgaggcctgg
     2761 acagcctcgg tgatccagcg ttcgacgaga gccacgccct cggtatcgac gacctgtacg
     2821 ccgagcggcg gcatgcgggc catccggttt tcactcttca tgcgcagcgt cagcatgtgc
     2881 gcaccgctgc cgtcggcggc gacgcgttgc gccgttgccg cgccttgtgg acggaaacgg
     2941 ctcgagtggc cgagcaacga tcgcagcgtc tgctcggcac tgtcgacgct tgccccggcc
     3001 gactgcgaca gcatcagctc gagcccgtcg agcgcgcccg acgcgctgtg gcagtgaccg
     3061 caattgccat gcaggtaccc gagcgccgca cgctcggtcg gtgtgcgcgc ggcaatccgc
     3121 accggggccg cgagcagcgc gggcgacagg ttcacgatcc ggcccagcgc aaccagcgag
     3181 cgcaggtcgg caaagtcacg gcgcggcgcg tcggcatgcg gcgccagcgg atcgcggtcc
     3241 gacgacaact gcagcgcact gaaacccagt accggcacgg ccgggccgtc atggcaggcc
     3301 aggcagtcgt tgcgcgatgg caccgagtag cgtccgcccg gcgcaccctc cgccgccacg
     3361 acggcgccgc cttccggcac cagcaccgca tctgtcccgt cggcgttcca tgcatagctc
     3421 gcgaagcgcc acgaaccatc ggggaggcgt tcgatcatgc gtgtctcgat gcgaccgccg
     3481 tagccgaact ccttccacag gcgggtgccg atcggaaatt gccaggcgtc gggctgcgag
     3541 gcattgattg cgcggccgcg cggcaggctg atccagcgct gtttgcgcgt tccgtccgac
     3601 cacagggggt actgcggcgc aaaggcgatg acctccgcac gaacgaccgt cgtcgaaccc
     3661 gccttgaaca atcctgtttc gctgagcagg cgcggcaact gaggtacggc cggctgcgcc
     3721 gccagcgcgg cagaaacggc cgccgtggcg atcagcgata ccagcaggtg cagggctttc
     3781 attgcggaga catccgggtt aggtcagtcc gcggaggtcg ccgaagagaa ctacagacgg
     3841 ccgtcgcggt gcttgctatc gaaggcgtgc acagtagcca gtcgaccgca acggcgttcg
     3901 taatactttg ctcagggatt cctaaaagac tcctaaaccg cggcggtccg ggcggcggga
     3961 ctcgccgccc ggccatgcga gggcgggcga gaaggaagga actgcgccgc tattcggggc
     4021 cgcgttgtac cgcgacctgc ttgcgtagcc aggtcatgaa cgtcgacacc tccggacgct
     4081 ttgcagcgcc cggggcgcct aggagccagt aggcacggcg cgcgagcgtg gcgtactgcc
     4141 gtcctttaag cggcaaggcg agccgtcgct ccccaaggaa cgggtcgagc agcggaaagc
     4201 gcccgagcgc caccccctgt cccgcgactg ccgcctgcac gacctggtcg taatgactga
     4261 acgtgatccc gcggctcgtg cgcggtcgcg gcacacccat cgcttcgcac cagaccttcc
     4321 acgacagcca cagcgcgatg ccgttcggat cctccagctc gatcagcggc aggcgcagca
     4381 ggtcctccac gctacggaca cgctgcccct tgagcaggga cggcgcggcc accggagcga
     4441 cctcttcgcc gaagagtcgt actgcgccag gtccggccgc ttcttccggg cagtagcgca
     4501 atgcaacgtc gagcgaactg cgttcgaggt cgcgcaccac gttgtcggcc accacatgca
     4561 ccgagatgtc ggcatgctcc ttctggaaac tcgcgagtcg cggcaccagc cacagcgagg
     4621 caaaggtcag cgtcgtcgtc acgcgcacga ccggcgtggc ctgcactcgc acggcggccg
     4681 tcgcctcgcg cagctggtcc agtaccttcg aaacgtcgcg gtagtagcgg tcaccggcgt
     4741 cggtcaggac cagcgcccgg gtacgtcgct cgaaaagctt cactcccagc tgctcctcga
     4801 gctgctgcac ctgccggctg atcgccgact gggtgaggaa gagctccgtc gctgccgccg
     4861 taaacgacag caggcgggcc gaggcttcga acccgcgcag cagatcgagc gacgtcggaa
     4921 gatcgcgttg tctatgcatt cccgtcactc atgcatcgca tcccggaatt tcgtttgtcg
     4981 ccgggcgccg gcggagcgac agtgtgcctc gtcatcaccg ctggagaaag accatgaccc
     5041 gcaccaacgc cgcagaaatc ctgagcagtg tcacgctcga cgtcgacgac cgtcc
//
LOCUS       DATIRT010000311         7986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1031925, whole genome shotgun sequence.
ACCESSION   DATIRT010000311 DATIRT010000000
VERSION     DATIRT010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7986
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031925"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(421..975)
                     /gene="flhC"
                     /locus_tag="VLN25_07100"
     CDS             complement(421..975)
                     /gene="flhC"
                     /locus_tag="VLN25_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HSQ10365.1"
                     /translation="MRIKSIVSESRQIQRAIALIKLGARLQVLESETDLSYERLLRLY
                     KEVQGESPARGMLPFSTDWFMTWQPNIHSSLFLNMYEYLDKSSELEEIDAIIKAYELY
                     LEQIRAQGLEPLLSVTRAWRLVKFVDAGMVTLTSCCKCGGKFVTHTFELTKDYVCGLC
                     EPPARAGKGKAQITTTVAADETLH"
     gene            complement(1001..1351)
                     /gene="flhD"
                     /locus_tag="VLN25_07105"
     CDS             complement(1001..1351)
                     /gene="flhD"
                     /locus_tag="VLN25_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HSQ10366.1"
                     /translation="MSTIAASNEQLLQEIREANLAYLVLAQHMIRADKAQALYRLGVS
                     EEVATIIDQLSPAQLMKIAGSNQLILRFRFDDDMVWNLLTSHSKSKSTAAGAAVAGLH
                     ANIVMAGQLADVTA"
     gene            1789..2043
                     /locus_tag="VLN25_07110"
     CDS             1789..2043
                     /locus_tag="VLN25_07110"
                     /inference="COORDINATES: protein motif:HMM:NF017237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HSQ10367.1"
                     /translation="MLEAARVEDWDEVGREEETCRRLIVRLKQAHADGRVAAERRQRL
                     ALLRMILADDAEIRELSEPWLKQLEALLTGGGRNDRPLAR"
     gene            2081..2830
                     /locus_tag="VLN25_07115"
     CDS             2081..2830
                     /locus_tag="VLN25_07115"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar regulator YcgR PilZN domain-containing
                     protein"
                     /protein_id="HSQ10368.1"
                     /translation="MGMTSPFPEPPSPSMARFVVRSRVEIVSALRQLRDLHLLVTLYY
                     GNDTAFAVGSVLDVHAGADELIVDLTVDAVARSAIAAAGSLVMVGFIDNVKLQFTTGR
                     GQALTPNGAAFRLPLPHEMLRLQRRTSLRVKTPALLCHVPRVIGSMQYYALRVSDLSL
                     GGLSLQGPCDRTLFALDRVIERCQIQTSSGRRVDVTLRVRHIEPLVGGRDAERIGCEF
                     VGLTAAVRNGLQQLIGSVALPAETPEMRRTD"
     gene            complement(2912..3343)
                     /locus_tag="VLN25_07120"
     CDS             complement(2912..3343)
                     /locus_tag="VLN25_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10369.1"
                     /translation="MTRAKLMKQLAEIATRPLVDTMHQPVTAERAPEAVERDLYVWFE
                     DTSARATEGRRGIAGLPESLLPYARGLRVVERRFAEGHMHLLAAVRLKCKGETADRIA
                     SLYTSALSVGTVAWIGTGTQYERTWIEMYGAKALLPRPATS"
     gene            3639..4358
                     /locus_tag="VLN25_07125"
     CDS             3639..4358
                     /locus_tag="VLN25_07125"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HSQ10370.1"
                     /translation="MYSYTSPRLFERHDYVRRYTPLVRRIAHQMVAKLPANVELDDMI
                     QAGMMGLMDAICRYEESQGTQFEVYASQRVRGAMLDELRANDWLPRSARKSQRDIENA
                     IHRLEGELMRAPHEAEIAEEMGLPIADYREMLSEARGSQLVYLDDLGSYDDDDDYLER
                     HFVVNGAEPSELLRDKRFRHALVAAIDELPEREKLLMSLYYEQDLNLREIGAVMGITE
                     SRVCQLHSQAVARLRSKLKNW"
     gene            complement(4431..4754)
                     /locus_tag="VLN25_07130"
     CDS             complement(4431..4754)
                     /locus_tag="VLN25_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10371.1"
                     /translation="MGDSPAAHLFADLLHALHDESDALVANDAARLIDAAHRKEHLLR
                     LLAPQANALRAMRRSGCDELERFAREAAQLAALDGGHAVSSLGASCGAGVAGRMAALR
                     DADPD"
     gene            complement(4758..5021)
                     /locus_tag="VLN25_07135"
     CDS             complement(4758..5021)
                     /locus_tag="VLN25_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10372.1"
                     /translation="MKTTLLPEALRYDWIGPLAGLPSPTVPCAENARGSGMGARLQVL
                     IAKGIDGVRVAAVQEIIRRGRYVTDTDELAAGVLAAIAPGRRH"
     gene            5288..>7986
                     /locus_tag="VLN25_07140"
     CDS             5288..>7986
                     /locus_tag="VLN25_07140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSQ10373.1"
                     /translation="MSGDRALALGVPPAGGRSAVAAPPDRSAFTAAPGLAYFEDCPDA
                     ILRFNAERRVVYANPAVERATAVSRWQFIEHRLEDVEHFADFAPLWNESLAAVLETQE
                     GRWFKFSYPHPTGPKLFDVRLQIETGVEAAHMHVTAVLRDITVPKSAMRATRAASEFV
                     EDLLASASIGISVLDRDCVYRVWNEHLETLLGVSADGVLGKAFHDAPGLAALSGLGIE
                     LQRLASGVSRIPTEVEARFPDGERPWVRVKLTPVFDANGRFDGVFITVERVDREHFAE
                     GSLTALRRALERVGEMVLEVDRHGTVVDANETALSTLGYERDALRGLSLGSIDAGMGA
                     GEFETLYEQLLARGAYQGEATYRTRFGSTFPVEVVVQRVEQSGREFILLLARDATERK
                     RAEDALTESAERFRALFDESPVAALLLDASFRVIGANRAASDTVGYPVEELMGHDPEM
                     LVLAEDQPGYRTMREQLLGDIVQSEAAERRFVHRDGRLVWSKLNVRSVAAGGGLRHYL
                     VVLENFTDRKIFEEQLQVALRDQQTLFETMSVGVAQTMSGKILLANREFSDMFGYRDG
                     QVIGMPLWDLTIDRDNRMPNEISGMPVVRANQTTSAEVVLFRKDGEPIWCLVQARPIQ
                     SETPGLESLREAIYTFQNISEMKRQREALSRSLLELNVVLDATSMGVMHLQDDRVVRC
                     NAQAQLMFGRPGAELVGTPVGALFSSEADYFEAITPLRAQLVTGSPASFEGLMRGRSG
                     TFWGLVSLRKVDAKVGAQGMIASILDISERKKQEEELHTALAQQQLIFDTALVGLLFV
                     RDGRPVRANSAMEDLLACEPGGLVKQIQLFTHPTDHLLLASLAEHYPRIRDTGACEFE
                     LYMYRRKADPIWVAVQGRAVNPERPELGYIFAFVDIDERKRSER"
BASE COUNT         1366 a         2486 c         2705 g         1429 t
ORIGIN      
        1 acagatttgt cgcctgcgct ccggagcggc cgagcgctgc gccgaggtac aaacccacgc
       61 actccacccc gcgctgcacg tcgcattgcc agggttgcct ctccctcctc gccgggcgag
      121 gagggtgcag ggaggagtgc gccattccct ctcgcttggc gagagggcca gggtgagtgc
      181 tgtgcagttg aaattcggcg aagcgcgcgg ccgctccgga gcgcagccga caaatctgtc
      241 tttggtcagt tccgccgcga gcgcacgtgc gaccgcgaag cggtcgcgta cgtgagcggc
      301 gtatcaaaaa agtggcccgg ttgcccgggc caaatagcgg aggaaaggaa tgccactcaa
      361 gaacgtaccg gtgaccgtgg cctccaccgg caatccgtcg gttgcccgac ggaattcaac
      421 tcagtgcagc gtctcgtcgg cagcgacagt cgtcgtgatc tgggccttgc ctttgccggc
      481 acgggccggg ggctcgcaca ggccgcagac ataatccttg gtcagctcga aggtgtgcgt
      541 gacgaacttg ccgccgcact tgcagcagga cgtcagcgtg accattccgg cgtcgacaaa
      601 cttcaccagg cgccaggcgc gagtcactga cagcagcggc tccagcccct gcgcacggat
      661 ctgctcgagg tacagctcgt acgccttgat gatcgcgtcg atctcttcga gctcgctgct
      721 cttgtccagg tactcgtaca tgttcaggaa gagcgacgaa tgaatgttcg gctgccaggt
      781 catgaaccag tcggtcgaga atggcaacat gccgcgcgcc ggcgattcgc cctggacttc
      841 cttgtacagg cgcagcaggc gctcgtagct caggtcggtc tcggactcca gcacctgcag
      901 gcgcgcgccc agcttgatca gcgcgatcgc acgctggatc tggcggctct cggaaacgat
      961 gctcttgatg cgcatgacgt agctccccgc gacggcctga tcaggcggtg acgtcggcca
     1021 gctggccggc catcacgatg ttggcgtgca ggccggcaac ggcggcgccg gcagcggtcg
     1081 acttgctctt gctgtggctc gtcagcaggt tccacaccat gtcatcgtcg aagcggaagc
     1141 gcaggatcag ctggttggag ccggcgatct tcatcaattg cgccgggctc agctggtcga
     1201 tgatcgtcgc gacctcttcc gagacaccga ggcgatacag cgcctgtgcc ttgtcggcgc
     1261 ggatcatgtg ctgggcaagc accaggtagg cgaggttggc ctcgcggatt tcctggagaa
     1321 gttgttcgtt gctggcggcg atggtgctca tgtgggtccc tcctgcttaa ggcccgattt
     1381 ggcgggcacg ttggttacga agtgacgcca ttgtgagaat ggatccgtcc gggttgaatc
     1441 ggggcactgc ccggcggcat gtcggcctgg gtccagtcca cgtgtaaggc tttttcctac
     1501 atcggtccgc ggcccctatg aaatggcagg agaacgcgta ttggcgggcg cccgttggcg
     1561 gatttgcgca tccagtggcg cagacgtgcc cgcttgaaaa gacgcaggat tgaccgtcca
     1621 cttccgcgat gtagaccgcg cgcggctcgc tagagtccac agcgtggagg acggcgccgc
     1681 gggcgccact atggggaaca gatgccgccg gacaatgggc aacgtgagaa caccgaacgg
     1741 tgctcgatga tcgatcacta tgaagcgatt gccggcgcca gtcggcgcat gctcgaagcc
     1801 gctcgcgtcg aggactggga tgaggtcggg cgtgaggaag agacctgccg ccgcttgatc
     1861 gtgcggctca agcaggccca cgcagacggt cgtgtcgccg ccgagcggcg ccagcggctc
     1921 gcgctgctgc ggatgatcct cgcggacgac gccgagatcc gggaactgtc ggaaccctgg
     1981 ctcaaacagc tggaagcgct gctgaccggc ggcggccgca acgacaggcc cttggcacgg
     2041 tgacggccgc gcgccggtcc gcaccgaagg cactgatccg atggggatga cctcgccgtt
     2101 tcccgagccg ccgtcgccat cgatggcgcg gttcgtggtg cgttcgcgtg tcgaaatcgt
     2161 gtcagcgttg cgacaactgc gcgatctgca cctgctggtg acgctgtact acggcaacga
     2221 caccgccttt gccgtcggca gcgtgctcga tgtgcatgcc ggtgccgacg aactgatcgt
     2281 cgatctgacg gtggatgccg tcgcccgttc agccatcgcg gcagccggct cgctggtcat
     2341 ggtcggtttc attgacaacg tgaagctgca gttcacgacc gggcgcgggc aagcgctcac
     2401 acccaacggc gcggcgtttc ggctgccgct accgcacgag atgttgcgac tgcagcgtcg
     2461 gacgtcgttg cgggtgaaga cgccggccct gctgtgtcat gtcccgaggg tcatcggttc
     2521 gatgcagtac tacgcactgc gagtgagcga tttaagtctc ggtggcctgt cgctgcaggg
     2581 accgtgtgac cgcactctgt ttgctcttga tcgtgtgatc gagcgttgcc agatccagac
     2641 ctccagcggc cgtcgtgtgg acgtcacctt aagggttcgt catatagaac cgctggtggg
     2701 cggacgggac gctgagcgaa ttggctgcga attcgtgggc ctcacggctg ccgtacgtaa
     2761 cggattgcag caactgatcg gtagtgtcgc gttgcctgcg gaaaccccgg agatgcgtcg
     2821 caccgactga ttcagggatt tctgcagtga ctcgaagtga cggcctcgac gaaattcgcc
     2881 gtcgacagtc gggcatgacg gggcgggcga ctcagctggt cgcgggacgc ggcagcaggg
     2941 ccttcgcgcc gtacatctcg atccaggtcc gctcgtactg tgtgccggtg ccgatccacg
     3001 ccactgttcc gaccgacagc gcggacgtgt aaagcgacgc gatgcgatcg gcggtttcac
     3061 ccttgcactt caaccggacc gcggcgagca ggtgcatatg gccctcggca aaccggcgct
     3121 cgacgacgcg caagccgcgt gcataaggca acagtgactc cggcagaccc gcgatgccgc
     3181 gccggccttc ggtggcacgg gccgacgtgt cctcgaacca cacgtacagg tcgcgctcga
     3241 ccgcctcggg cgcacgttcc gcagtcacgg gctgatgcat tgtgtccacc agcgggcgtg
     3301 tggcgatctc ggcgagctgc ttcatgagtt ttgcgcgtgt cattgggagt gtccgtgggc
     3361 gagcgtccgg ttgctgcatg aaccattgca tcggttgacg gactcgcgcg cttgagcagg
     3421 acgccagcgg gaatgggacc gcaacagggc gcatttcgca ccgcgccgac gagcggtgga
     3481 tcgccctcat ccgaacgact gcgtgctcga ggaaatttca ccggcccccc ttaaatgtcc
     3541 gggcttcttg ccgatattcg ccgcttcgcg aaatgcagcg gccccgaaga atgcggctca
     3601 tcgggtcagt ggggtaggtc gtgaaaaacg aagcgttcct gtacagctac acgtctccgc
     3661 gactgttcga gcggcacgac tatgtgcgtc gttacacacc gctggtacgt cgcatcgcgc
     3721 atcagatggt cgccaagctt ccggccaacg tcgagctcga cgacatgatc caggccggca
     3781 tgatgggact gatggacgcg atctgtcgct acgaggagtc gcaaggcacg cagttcgagg
     3841 tgtatgcctc gcagcgagtg cggggcgcaa tgctggacga gctgcgcgcc aatgactggc
     3901 tgccgcgctc tgcacgcaag agccagcgcg acatcgaaaa tgccatacac cggctcgaag
     3961 gcgagctgat gcgtgcgccg catgaagccg aaatcgccga ggaaatgggt ctgccgattg
     4021 ccgactatcg cgagatgctc agcgaagcgc gcggatccca gcttgtgtat ctcgacgatc
     4081 tcggcagcta cgacgacgat gacgactacc tggagcgcca cttcgtcgtc aacggagccg
     4141 agcccagcga actgttgcgc gacaagcgct tccgacatgc gctggtcgcg gccatcgacg
     4201 aactgccgga gcgcgaaaag ctgctgatga gcctgtacta cgaacaggac ctgaatctgc
     4261 gcgaaatcgg cgccgtgatg ggcatcaccg agtcgcgcgt ctgccagctg cacagccagg
     4321 cggtggcccg ccttcggtcc aagctcaaga actggtagtc aggatttggc ctgactgcgt
     4381 gccgctggcg cggacaggaa aggtcgatgt ccgtgccatc gcgcaggcga tcaatcggga
     4441 tccgcgtcgc gtagggcggc catacgcccc gcgactcccg caccgcatga agcccccagg
     4501 ctactgaccg catgaccccc atcgagcgcc gccagctgcg ccgcttcgcg cgcgaatcgc
     4561 tccagttcat cgcaaccgga tcgccgcatc gcgcgcagcg cgttggcctg cggggccagc
     4621 agacgcagca ggtgttcctt gcgatgagcg gcatcgatca ggcgcgcagc atcgttggcg
     4681 accagcgcat cgctttcgtc gtggagcgca tggagaagat cggcaaacag atgcgcggcc
     4741 ggactgtcgc ccatggctca gtgcctccgg cccggagcga tcgcggcgag gacgcccgca
     4801 gccagctcgt cggtgtcggt gacatagcgg cctcgcctga tgatttcttg cacggccgcg
     4861 acgcgaacgc cgtcgatgcc cttagcaatg agcacctgga ggcgggcccc cattccagac
     4921 ccgcgggcgt tctcggcgca ggggacggtt ggggagggca ggccggccag cggaccgatc
     4981 cagtcgtatc tgagggcttc gggaaggagg gtcgttttca taggcgcgtc acacagtcga
     5041 tgcgtcggag ttcggcactg cagaggcgat ctttagtcac ccgtttcggc aaatgggggt
     5101 cccgcggcct tccggtggtg gctgccgagg cctgcgtaca cccgttccgc ggcaggcgtg
     5161 gagtattctt gaatcgcctt catctaccct gcgccgccca cgcgtgtccg acactacgac
     5221 ccggctccga cgacccgcgt cgccttcaac gcggcgaggc gcggttgcct tggtcgcgcc
     5281 ggatgtgatg tcaggcgacc gcgcgcttgc gctgggcgtt ccgcccgcgg gcggacgtag
     5341 cgccgtcgcg gctccgccgg accggtctgc gttcacagcg gcccccggcc ttgcgtactt
     5401 cgaggactgc ccggacgcca tccttcgctt caatgccgag cgccgggtcg tctatgcgaa
     5461 tcctgcggtc gagcgcgcaa cggccgtgtc gcgctggcag ttcatcgagc atcggctcga
     5521 ggatgtcgag cacttcgccg atttcgcgcc actgtggaac gagagcctgg ccgccgtgct
     5581 ggaaacgcag gaggggcgct ggttcaagtt ttcgtacccg cacccgacgg gaccgaagct
     5641 cttcgatgtg cggctgcaga ttgaaacagg ggtagaagcg gctcacatgc atgtgaccgc
     5701 ggtgctgcgc gacatcaccg tgcccaagtc ggcgatgcgc gcgacacgcg cggcgtccga
     5761 attcgtcgag gacctgctgg cgagcgccag cattggcatc agcgtgctcg accgcgactg
     5821 tgtctatcgg gtatggaacg aacatctcga aacgctgcta ggcgtgtcgg ccgatggagt
     5881 cctgggcaag gcctttcacg acgccccggg gctcgccgcg ctgtccgggc tcggtatcga
     5941 actgcagcgg ctggcgagtg gcgtgtcccg cattccgact gaggtcgaag cccgcttccc
     6001 cgatggcgag cggccgtggg tccgggtcaa gctcaccccg gtcttcgatg cgaacggtcg
     6061 cttcgatggc gtgttcatca cggtcgaacg tgtcgatcgt gagcactttg ccgagggttc
     6121 gctgaccgca ttgcgacgcg cgctcgagcg cgtcggcgag atggtgctcg aagtcgaccg
     6181 gcacggcacc gtggtcgacg ccaatgagac agccctttcg acgctgggtt acgagcgcga
     6241 cgccctgcgc ggcctgtcgc tgggttcgat cgatgctggc atgggcgcgg gcgaattcga
     6301 aacactctac gagcagttgc tggcgcgcgg cgcctaccag ggcgaagcga cgtaccggac
     6361 gcggttcgga tcgaccttcc cggtcgaagt ggtggtgcag cgggtcgagc agagtggccg
     6421 cgagttcatc ctgctgctgg ctcgcgatgc cacggaacgc aagcgggccg aggacgcgct
     6481 gacagagagc gccgagcggt tccgggcgct gttcgacgaa agcccggtgg cggcgctcct
     6541 gctcgatgcg agcttccgcg tgatcggcgc caatcgtgca gcgagcgaca ccgtcggtta
     6601 tccggtcgag gaactgatgg ggcacgaccc ggagatgctg gtgcttgctg aagatcagcc
     6661 cggctatcgc acgatgcgcg aacagttgct cggcgacatc gtgcagagcg aggcggcgga
     6721 acgccgcttc gtgcatcgcg acggccggct ggtctggtcg aaactgaatg tccgctcggt
     6781 ggccgcaggc ggaggcttgc gtcactacct ggtcgttctc gaaaacttca ccgatcgcaa
     6841 gatcttcgag gagcagctgc aggtggcgct gcgcgaccag cagacgctgt tcgaaacgat
     6901 gtcggtcggc gtggctcaga ccatgtccgg caagatcctg ctggcgaacc gcgaattttc
     6961 cgacatgttt ggctaccgtg atggccaggt catcggcatg ccgctgtggg acctcaccat
     7021 cgatcgtgac aaccgcatgc cgaacgaaat ctcgggcatg ccggtggtgc gcgccaacca
     7081 gaccaccagc gccgaagtgg tgctgttccg caaggacggg gagccgatct ggtgcctggt
     7141 gcaggcgcgc ccgatccagt ccgagacgcc gggtctcgag tcgctgcgcg aggcgatcta
     7201 caccttccag aacatctcgg agatgaaacg gcagcgcgag gcgctgagcc gctcgctgct
     7261 ggagctcaac gtcgttcttg acgccacttc catgggcgtg atgcacctgc aggacgatcg
     7321 ggtcgtgcgc tgcaatgcgc aggcgcaact gatgttcggg cgtccgggcg cggagctggt
     7381 cggaactccc gtcggagcgc tgttttcgtc cgaggccgat tacttcgagg cgatcacgcc
     7441 gctgcgtgcg cagctggtga cggggagtcc ggcgtccttc gaaggactga tgcggggccg
     7501 cagcgggacg ttctggggcc tggtttcact gagaaaggtg gatgcgaagg ttggtgcgca
     7561 gggaatgatc gcgtcgatcc tggacatctc cgagcgcaag aagcaggagg aggagctgca
     7621 tacggcgctg gcgcagcagc agctgatctt cgacacggcg ctggtgggac tgctgttcgt
     7681 gcgcgatggc cggccggtgc gggccaacag cgcgatggag gatctgcttg cctgcgagcc
     7741 cggcggcctg gtcaagcaga ttcagctgtt cacgcacccg accgatcacc tgctgctggc
     7801 cagcctggcc gaacactatc cgcgcatccg cgataccggc gcctgcgagt tcgagctcta
     7861 catgtaccgg cgcaaggccg atccgatctg ggtcgcggtg caggggcgcg ccgtcaatcc
     7921 ggagcgtccg gaactcggct acatcttcgc cttcgtcgac atcgacgaac gcaagcgttc
     7981 ggagcg
//
LOCUS       DATIRT010000312         3378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_933383, whole genome shotgun sequence.
ACCESSION   DATIRT010000312 DATIRT010000000
VERSION     DATIRT010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933383"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VLN25_07145"
     CDS             <1..727
                     /locus_tag="VLN25_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HSQ10374.1"
                     /translation="DLGFAQSDVQFNAAKGVAQFKEGGAYGDLRAVFSVHPEPFTVLA
                     RKEAGIKTFADFKGKRYNVGNPGSGTRASMEELLGAMGWKLGDFSLASELKADEHGPA
                     LCDGKIDGFFYAVGHPSANIQDPTTICGAKLVSLTGPAVDALVKDKPYYALATIPGGM
                     YPNNPNPTTTYGVQATIVSSAKVPADVVYAITKAVFDNLDEFKKLHPALANLKPEDMI
                     KNGLSAPLHEGAVRYYKEKGWIK"
     gene            865..>3378
                     /locus_tag="VLN25_07150"
     CDS             865..>3378
                     /locus_tag="VLN25_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HSQ10375.1"
                     /translation="MQLGDTDVQQLVKDADLGGRSTVGVAGTVMMVVGLVWVAFQLWY
                     ASPLPFALRFGVFNDTEARSIHLAIGLFLAFLAFPAFKRSPRDYVPWQDWIMAIAGAL
                     TSLYLIFFYGELALRPGNPTMLDVIVGTAGVLLLLEATRRAVGLPMAVLATLFLVYVM
                     AGPHLPDVIAHKGASLPRVVSHMWMVTEGVFGIALGVSVSFIFVFVLFGTLLDRAGGG
                     NYMMQVSFALLGHLRGGPAKVAVVSSALNGLISGSSVSNVVSGGIFTIPLMKRAGYGG
                     VKAAAIETSSSVNGQIMPPVMGAAAFLMVEYVGIPYSDIVKHAFIPAVISYIALFYIV
                     HLEALKLGMKPTARERDRTMGNRLTSWAMGLAGSAVALGVIYYIALGAQALFGSAAPY
                     ILGVLLAALYVYSVWVAARQPDLPLEIDVENPVLPPTWPTVKAGLHFLIPVGVLIWCL
                     MVEELSPGLSAFWGVATQMILMATQRPLIAIFRKQGELGGQFKAGMHDVLVGCNEGAR
                     NMIGIAVATGCAGIIVGAITLTGLGLRMTDFVEFVSQGNVLVMLLFTAFVCLVLGMGV
                     PTTANYILVATLMAPVIVELGAQSGLVIPLIAVHLFVFYYGIMGDITPPVGLASFAGA
                     AIAKEDPIAVGIQGSVYAVRTVVLPFIWIFNPALLLIDVHGWGEALLVAAASTVASLL
                     FAAATMAWFRIKCRWWEIVLLLVATFILFRPDYFADWFGPEYTDAPTAKFYEVVNGLD
                     DKDRLVFKIRGQSLEGEDKNKTVGLQLGPKLGDGSNPIDDARKRLAEAGMSVSGMGEM
                     MQVSTVRFGSRASKARIEQGYDIVGVKVPTERINSHWFYI"
BASE COUNT          572 a         1050 c         1105 g          651 t
ORIGIN      
        1 cgacctgggc ttcgcccagt ccgacgtgca gttcaacgcc gccaagggcg ttgcccagtt
       61 caaggagggt ggcgcctacg gcgacctgcg tgcggtgttc tcggtccacc cggagccctt
      121 cacggtgctc gcccgcaagg aagcaggcat caagaccttc gccgatttca agggcaagcg
      181 ctacaacgtc ggcaaccccg gctcgggcac gcgcgcgtcg atggaggaac tgctgggcgc
      241 gatgggctgg aagctgggcg acttctcgct ggcttcggag ttgaaggccg acgaacatgg
      301 ccccgcgctg tgcgacggca agatcgacgg cttcttctac gccgtgggcc atccgtcggc
      361 caacatccag gacccgacca cgatctgcgg cgccaagctg gtttcactga ccgggccggc
      421 cgtggacgcc ctggtgaagg acaagccgta ttacgcgctc gcgacgattc cgggcggcat
      481 gtatccgaac aacccgaatc cgaccacgac ttacggcgtg caggcgacca ttgtgagctc
      541 ggccaaggtg ccggccgacg tcgtctacgc aatcaccaag gcggtcttcg acaacctcga
      601 cgagttcaag aagctgcatc cggcgctggc caacctgaag ccggaagaca tgatcaaaaa
      661 cggcctgtcg gcgccgctgc atgaaggtgc cgtgcgctac tacaaagaga agggctggat
      721 caagtaagca ccaccgcggg ccgatgcgcc atcggctcgt ggggcgcgta aagttcgacg
      781 ggcggcctcg gccgcccgtt ttcttttgag ggagcgttcg tgaacaacaa gaagctcgaa
      841 gaggaactcg ccccgaccgc cgaaatgcaa ctgggcgaca cggacgtcca gcaactcgtc
      901 aaggacgccg acctgggcgg acgttcgacg gtcggagtgg ccggcacggt gatgatggtg
      961 gtcggcctgg tctgggttgc attccagctc tggtacgcat cgccactgcc gttcgcactt
     1021 cgcttcgggg tcttcaacga caccgaagca cgctcgatcc accttgcgat cggactgttc
     1081 ctcgccttcc ttgccttccc ggccttcaag cggtcgccac gcgactacgt gccatggcag
     1141 gactggatca tggccattgc cggcgccctg acgtcgctgt acctgatctt cttctacggc
     1201 gaactggcat tgcggccggg caatccgacc atgctcgatg tcatcgtcgg cacggcgggc
     1261 gtgctgctgt tgctggaggc gacgcgccgt gccgtaggac tgccgatggc ggtactggcg
     1321 acgctgttcc tggtgtacgt catggcagga ccccacttgc ccgacgtcat cgcgcacaag
     1381 ggcgcgtcgc tgccgcgtgt tgtctcgcac atgtggatgg tgaccgaagg tgtgttcggc
     1441 atcgcgctcg gtgtgtccgt gagtttcatc ttcgtgttcg tgctgttcgg cacgctgctc
     1501 gatcgcgcgg gcggcggcaa ttacatgatg caagtgagtt ttgcgctgct cggacacttg
     1561 cgcggcggcc cggcaaaggt cgccgtggtg tcgtcggcac tgaacggcct gatctccggc
     1621 tcgtcggtca gcaatgtcgt gtcgggcggg atcttcacca ttccgctgat gaagcgcgcg
     1681 ggctacggcg gtgtcaaggc ggcggcgatc gagacgtcgt cgtcggtgaa cggccagatc
     1741 atgccgccgg tgatgggtgc cgccgcgttc ctcatggtcg agtacgtcgg catcccctac
     1801 tcggatatcg tcaagcacgc attcatcccg gcggtgatca gctacatcgc cctcttctac
     1861 atcgtgcacc tcgaagcgct gaaactcggc atgaagccga ccgcgcgcga acgcgaccgc
     1921 acgatgggca accggctgac gtcatgggca atgggactgg ccggcagtgc cgtcgcgctc
     1981 ggcgtcatct actacatcgc cctcggcgcg caggcgctgt tcggaagtgc agcgccgtac
     2041 atattgggcg tgctgctcgc cgcgctgtat gtctacagcg tctgggtcgc agcgcggcag
     2101 cccgacttgc cgcttgagat cgatgtcgaa aatccggtcc tgccgccgac atggccgacg
     2161 gtcaaggccg ggttgcactt cctgattcca gtgggcgtac tgatctggtg cctgatggtc
     2221 gaggaactgt cgccgggact ttcggccttc tggggcgtgg ccacccagat gatcctgatg
     2281 gcgacgcagc ggccgctgat tgccatcttc cgcaagcagg gtgaactcgg aggacagttc
     2341 aaggcgggca tgcacgacgt gctggtgggc tgcaacgaag gcgcgcgcaa catgatcggc
     2401 attgcggtgg ccaccggctg cgcgggcatc atcgtcggtg cgatcacgct gaccgggctc
     2461 ggcctgcgta tgaccgactt cgtcgaattc gtctcgcagg gcaacgtgct ggtgatgctg
     2521 ctcttcactg cgttcgtctg cctcgtgctg ggcatgggcg tgccgacgac agccaactac
     2581 atcctggtgg cgacgctgat ggcgccggtc atcgtcgaac tcggcgcgca gtcgggtctg
     2641 gtcattccgc tgatcgccgt gcacctcttc gtcttctact acggaatcat gggcgacatc
     2701 actccgccgg tggggctggc gagtttcgcc ggtgcggcga tcgcgaagga agatcccatc
     2761 gccgtcggca tccagggttc ggtctatgcg gtgcggaccg tcgtgttgcc cttcatctgg
     2821 atcttcaatc cggcactgct gctgatcgac gtccatggct ggggcgaggc gctgctggtc
     2881 gctgctgcat ccactgtggc atcgctgctc tttgccgccg ccacgatggc ctggttccgc
     2941 atcaagtgcc gctggtggga aatcgtcctg ctgctggtcg cgacgttcat cctgtttaga
     3001 cccgactact tcgcggactg gttcggcccg gaatacaccg acgcgccaac agcgaagttc
     3061 tacgaggtag tgaacggcct ggatgacaag gatcggctgg tcttcaagat ccgcgggcag
     3121 tcgctcgagg gcgaggacaa gaacaagacc gtgggactgc aactcggccc gaagttgggc
     3181 gacggcagca acccgattga tgacgcgcgc aagcgcctgg cggaagccgg catgtcggtg
     3241 tccggcatgg gcgagatgat gcaggtgagc accgtgcgct tcggcagccg cgcctccaag
     3301 gcacgcatcg aacagggtta cgacatcgtc ggcgtcaagg taccgaccga acgcatcaac
     3361 tcgcactggt tttacata
//
LOCUS       DATIRT010000313         9933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_343563, whole genome shotgun sequence.
ACCESSION   DATIRT010000313 DATIRT010000000
VERSION     DATIRT010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9933
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343563"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1460
                     /locus_tag="VLN25_07155"
     CDS             <1..1460
                     /locus_tag="VLN25_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HSQ10376.1"
                     /translation="VVGAISVITVSTFYLTAQMVGGVVLVKTLIGIDYEISVIVVGIL
                     MLGYVLFGGMVATTWVQIIKAVLLVTASILLVMFVWLPYGFSLPGFLQAAIGDAKVQG
                     QVATLLGDASKNMTPAELGQRFLEPGLLFKAPIDQISLGMALVFGTAGLPHILMRFFT
                     VPTAQEARKSVIWAMAIIGGFYVLTLFLGLGAAMNVGAANIRAVDAGGNMAAPLLAQF
                     VGGGPDSFAGNLFLAFVAAVAFSTIVAVVAGLVLAAASAMAHDLYVGVIRSEHVTPQE
                     QVKAARIATVIVGAMAIAIGIAAKGQNVAHLVALAFAVAASANFPCVLLTLYWKRCNT
                     GGIMAGMVIGTLTAIGLVLVSPNMTYPKAIKASAQKVVDGAPARLATLNEALASGDAA
                     KVAKAGTDKASIEKAVAAAQANLDRFQNDNTSMVSLEAPLFQLKNPGLISIPLGFLAV
                     ILGSLLYRDKRAEEMWDELYVRQNTGILSAKAHAH"
     gene            1631..3529
                     /locus_tag="VLN25_07160"
     CDS             1631..3529
                     /locus_tag="VLN25_07160"
                     /inference="COORDINATES: protein motif:HMM:NF015410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HSQ10377.1"
                     /translation="MNNPTTPPLVYESVREFLVRHAPFARMSEDALRYLIPRLKLAYF
                     AKDSVVVDRSAAFPPLHIIQNGHVASVTAGLDTMPDRVLQPGESFPVGALSAGGQPTR
                     SYRATDDVFTYLLPVDDFQRLRQLSPEFAAYCTEAVTVLAQQSLAELQRHYSQVAADQ
                     HSLTRPLAQLIPSEPVTCLASTPLREALRQMREHAVRSIIVVDEASAPIGVFTLNDLR
                     DRVVLQELPLDTPVARVMTPDPVTLPSQATAAEAIQSMAAGGFHQMIVTSHGKVLGTI
                     FEHDLFALQRVSLRQIHHAIRSARSVKQLAHVAGDIRNLARNLLAQGVSAEALTRTVA
                     SLNDALTREVINRVLQEHALDGIDWCWLALGSEGRGEQTLATDQDNALIFVAPGDPTE
                     LLRMRHELIVFAREVNAALDQLGFPLCKGNIMASNPQWCLTESEWRAQFTTWLTEPTP
                     ERLLNANIFFDFRALYGDSTLADRLSDWLLARTQDNKLFLRLMVQNALETEPPLGLIR
                     AFQVSDKPGREGTFDLKKRGVRIFVDAARVFMLAYGVKAAGTADRLRKAGETLRADPR
                     HVEATVEAFHYLQVLRLRAQEHPHATNPNRIDPYALNEIDQRMLKEAFRQARKLQQRL
                     KETFSIAL"
     gene            3586..4281
                     /locus_tag="VLN25_07165"
     CDS             3586..4281
                     /locus_tag="VLN25_07165"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HSQ10378.1"
                     /translation="MGLLSRLLRSRPSLGPELEVRLQRLSELSPPPTRSTHRQSRYVT
                     VDVETTGLDMRHDHVLSIGAVAVEHGVIALAHCFEVVLRQAESSSHDNILVHRIGGQR
                     QLGGEDPAESFVRFLEYVGHCPLVAFRAEFDQTMLDRALKEHLNIVSQSQWLDLAELL
                     PALYPSNENKTMDDWLAAMNIHMLARHDALADALATAQMLQVCLRQAESLEMTCPAHL
                     LEMQRAQHWLGKR"
     gene            4497..5780
                     /gene="hemA"
                     /locus_tag="VLN25_07170"
     CDS             4497..5780
                     /gene="hemA"
                     /locus_tag="VLN25_07170"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HSQ10379.1"
                     /translation="MHLLTLGLNHTTAPLAVRERVAFVPEEVCTTIGRLREKLSEPSN
                     GRVSEAAIVSTCNRTELYCAAEEPDRAQRALQEFVAAEKQLSLDELQRHTYVLPHASA
                     VRHAFRVASGLDSMVLGEPQILGQMKQAEKLARTAGGLGLMLNHLFQRTFAVAKEVRT
                     TTEIGTQSVSMAAAAVRVAQRIFGNLSGEHILFVGAGEMIELTATHFAAQHPKSITVA
                     NRTQERAQALAHRFHANAIKLAELPDMISRFDIVVSCTASSLPIIGLGMIERAIKSRR
                     RKPMFIVDLAVPRDVEPEVARLDDVYVHTVDDLGRIVQSGSESRQAAVAQAEAIIESR
                     VRDFEEWVKARRAVPVIQDLRTRADALRRRELERALKQLARGEAPEAVIEHLSQALTN
                     KFLHDPMSTLREVGDDAEQARLQALLARFYSQSDD"
     gene            5934..7016
                     /gene="prfA"
                     /locus_tag="VLN25_07175"
     CDS             5934..7016
                     /gene="prfA"
                     /locus_tag="VLN25_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HSQ10380.1"
                     /translation="MKTSMQTKLQQLARRLEEIDVQLSSEDAARDMNRYRALGQERSE
                     IEPVVTRFHEYNRAHADLLGAQEMLSDPDMKTLAEEETHVARGRMESIESDLQRLLLP
                     QDPNDDRNIFLEVRAGTGGDESGLFAGDLLRMYMRFAERHRWTTEMISESPSDLGGYK
                     EAIVRIVGTGAYSKLKFESGGHRVQRVPETEAQGRIHTSACTVAVLPEADEINEIRID
                     PSEIRIDVYRASGAGGQHVNKTESAVRITHLPTGIVVECQDDRSQHKNRERAMKILAA
                     RIKDQQTREQQAKEASARKSLIGSGDRSERIRTYNFPQGRVTDHRINLTLYKIDQIMD
                     GDIDELTERLAAEHQAELLAALGESA"
     gene            7017..7823
                     /gene="prmC"
                     /locus_tag="VLN25_07180"
     CDS             7017..7823
                     /gene="prmC"
                     /locus_tag="VLN25_07180"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HSQ10381.1"
                     /translation="MRTADSLLRSADLPRTEARALLASALRVTRERLIAFPETEVSPT
                     ALAAFEALAARRREGEPLAYLLGEKEFYGRRFAVTPDVLVPRPDTESLVEVALECLSN
                     VMAPRVLELGTGSGCIAVTLQLERPDARVVATDVSAVALAVARTNAQDLKAPVTFRKG
                     DWYGAVDSAEPFDLIVSNPPYVAAADPHLDALKHEPAMALSDGHDGLQCLSTLIAGAR
                     AHLYASAWLVLEHGFDQSLAVARLLREAGFREVSVRCDAGGQPRVSYGRS"
     gene            8178..8498
                     /gene="grxD"
                     /locus_tag="VLN25_07185"
     CDS             8178..8498
                     /gene="grxD"
                     /locus_tag="VLN25_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HSQ10382.1"
                     /translation="MDAKEFIQKTVTDHPVVLFMKGTAQFPQCGFSGRAIQVLKQAGA
                     PSLVTVNVLDDDDVRQGVKDYANWPTIPQLYVNGQFVGGSDIIMEMFQSGELQTLLSQ
                     AKAA"
     gene            8498..9085
                     /locus_tag="VLN25_07190"
     CDS             8498..9085
                     /locus_tag="VLN25_07190"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HSQ10383.1"
                     /translation="MAAPARRLIVAITGASGAIYGVRLLRRLQERTDVETHLLLSSAG
                     VLNVYQEMDLKRGDVEALAHVVHSVNDVGATIASGSFATAGMVIAPCSMKTLAAIAHA
                     YADTLITRAADVCLKERRRLVLLTREAPLNLAHIRNMEAVTLMGGIIFPPVPALYHRP
                     TSIDEMVDHTVSRVLDLFDLEDSKSPRWSGALGPT"
     gene            complement(9347..9880)
                     /locus_tag="VLN25_07195"
     CDS             complement(9347..9880)
                     /locus_tag="VLN25_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10384.1"
                     /translation="MFGTFVVGSESDGRLRLNSADGGLTQNANGQDRLNLRAALNTNT
                     DSSDLGTGLKIDDTLGLTGLFDLSSLTGPLINGYGIRFTDRNADGVKQAAELDVRFNP
                     VTGKIEVRYLLQDFDTNEIFERGLADFAPPNGADQILLQLTRPDTTNTNFFASYSYLQ
                     GGNVLGGGAFDAPISLF"
BASE COUNT         1790 a         3270 c         3169 g         1704 t
ORIGIN      
        1 gcgtggtcgg agcgatctcg gtcatcaccg tgtctacctt ctacctgacg gcgcagatgg
       61 tcggcggcgt cgtgctggtc aagacgctga tcggcatcga ctacgagatc tccgtcatcg
      121 tcgtcggcat cctgatgctg ggctacgtgc tgttcggcgg catggtcgcc accacctggg
      181 tgcagatcat caaggccgtg ctgctggtca ccgcgtcgat cctgctggtg atgttcgtct
      241 ggctgcccta cggtttctcg ctgcccggct tcctgcaggc cgccatcggc gatgccaagg
      301 tgcagggtca ggttgccact ctgctaggcg acgccagcaa gaacatgacg ccggccgaac
      361 tcggacaacg cttcctcgaa ccgggtctgc tgttcaaggc tccgatcgac cagatctcgc
      421 tcggcatggc gctggtgttc ggcacggccg gattgccgca catcctgatg cgcttcttca
      481 cggtcccgac cgcgcaggaa gcgcgcaaga gcgtgatctg ggcgatggcg atcatcggcg
      541 gcttctacgt gctgacgctg ttcctcggcc ttggtgcggc gatgaatgtc ggtgcggcca
      601 acatccgcgc cgtcgatgcc ggcggcaaca tggcggcgcc cttgctggca cagttcgtcg
      661 gtggcggccc ggacagtttc gctggaaacc tgttcctcgc cttcgtagcg gctgtggcgt
      721 tctctaccat cgtggccgtg gtcgcgggcc tggtgctggc ggccgccagc gcgatggcgc
      781 acgacctgta cgtcggtgtg atccgcagcg agcacgtcac gccgcaggag caggtcaagg
      841 cggcgcgcat cgcaaccgtc atcgtcggcg caatggcgat tgcgatcgga atcgcggcga
      901 agggtcagaa cgttgcgcac ctcgtggcac tggcgtttgc cgtcgccgcc agcgcgaact
      961 tcccgtgtgt actgctgacg ctctactgga agcgatgcaa taccggcggc atcatggccg
     1021 gcatggtcat cggcactctg acagccatcg gcctggtgct ggtatccccg aacatgacct
     1081 atccgaaggc catcaaggcc tccgcgcaga aggtcgtcga cggtgccccg gcccggctgg
     1141 ccacgctcaa cgaggcgctc gcgtcaggcg atgccgccaa ggtcgcgaag gccggaacgg
     1201 acaaggcctc gatcgagaaa gccgtggcgg ccgcacaagc gaatctggac aggttccaga
     1261 acgacaacac cagcatggtc agtctcgagg cgccgctgtt ccagctgaaa aatccgggcc
     1321 tgatctcgat cccgctgggc ttcctcgcgg tgatcctcgg gtcgctgctg tatcgcgaca
     1381 agcgcgccga ggaaatgtgg gacgagttgt atgtgcgcca gaacacgggc attctctccg
     1441 ccaaggcgca cgcccactaa gtcgaaacga tgccgctcga cgaagcaggg ccccgatcgt
     1501 ggggcccttt ttcttccttc cgaaactgcg cgaacttgcg agaacatgcg cgcactcgga
     1561 ttgatgcttc gccgcggccg cggctccgtc gatccggctt acgcgggtcg caaccttcac
     1621 gcctgatgcc gtgaacaatc ccacgacacc gccgctcgtc tacgagtcgg tgcgggaatt
     1681 cctggtccgg cacgcaccgt ttgcgcgcat gagcgaggat gcgctgcgct atctgatccc
     1741 gcgcctgaag ctcgcctact ttgcgaagga cagcgtggtc gtcgaccgca gtgcggcgtt
     1801 cccgccgctg cacatcatcc agaacggcca tgtcgccagc gtcactgccg gtctcgacac
     1861 catgccggat cgcgttctgc agccgggtga aagtttcccg gtcggtgcgc tctcagcggg
     1921 cggccaaccg acccgcagtt atcgtgccac cgacgatgtc ttcacgtatc tgctgccggt
     1981 cgacgatttt cagagactga ggcaactctc tcccgagttt gctgcgtact gtaccgaggc
     2041 ggtcaccgtg ctcgcgcagc agtcgctcgc ggaactgcag cggcactaca gtcaggtggc
     2101 cgccgaccag cactcgctga cacgtccgct cgcccagctg atcccgtccg agcctgtcac
     2161 ctgcctggcg agcacgccgc tgcgcgaggc gctgaggcag atgcgggagc acgcagtgcg
     2221 atcgatcatc gtcgtcgacg aggcctcggc accgatcggc gtgttcaccc tgaacgacct
     2281 gcgcgaccgt gtggtgctgc aggagttgcc cctggacaca ccggtcgcgc gcgtgatgac
     2341 gcctgatccg gttacgttgc cttcgcaagc cacggcggcc gaggccattc agagcatggc
     2401 cgccggtggc ttccaccaga tgatcgtcac ttcacacggc aaggtgctcg gcaccatctt
     2461 cgagcacgac cttttcgctt tgcagcgagt gagcctgcgg cagattcatc atgccatccg
     2521 ctctgcgcgc tcggtcaagc aactcgctca cgtggcgggc gacatccgca acctcgcgcg
     2581 caacctgctg gcgcaggggg tgtcggccga agcactgacg cgtaccgtcg cttcgttgaa
     2641 cgacgcgctg acgcgcgaag ttatcaaccg cgtgctgcag gagcatgcgc tcgacggcat
     2701 tgactggtgc tggctcgcac tcggcagtga aggccgcggc gagcagacac tggccaccga
     2761 ccaggacaat gcgctgatct tcgtcgcacc gggcgatccg accgagctgc tcagaatgcg
     2821 ccacgagctg atcgtgttcg cccgcgaagt caacgcggcg ctcgaccagc tcggctttcc
     2881 gctgtgcaag ggcaacatca tggcgagcaa cccgcagtgg tgcctgacgg agtccgagtg
     2941 gcgagcccag ttcacgacgt ggctgaccga accgacgccg gagcgcttgc tcaacgccaa
     3001 tatcttcttc gatttccgag cgctctacgg cgatagcacc ctggcggacc gtctgtccga
     3061 ctggctcctg gcaaggacgc aggacaacaa gctgttcctg cgattgatgg ttcagaacgc
     3121 tcttgaaacc gagccgcccc tcggccttat ccgcgcgttc caggtttccg acaagcccgg
     3181 ccgtgaagga acgttcgatc tcaagaagcg cggcgtccgc atctttgtcg atgccgcacg
     3241 cgtgttcatg ctcgcctacg gcgtgaaggc cgcaggcacg gccgaccgcc tgcgcaaggc
     3301 cggtgaaacc ctcagggccg atccacgcca tgtggaggcg acggtcgagg cgtttcacta
     3361 cctccaggtc cttcggcttc gagcgcagga acacccgcac gcgaccaacc cgaatcgcat
     3421 tgatccgtac gcgctcaacg aaatcgacca gcgcatgctg aaggaggcgt ttcgtcaggc
     3481 ccggaagctg cagcagcggc tgaaggaaac gttttccatc gcgctctgac accacgcgcc
     3541 ctggaccacc acacggcgca gcatcggacc acggacgacg ggtgcatggg actgctgagc
     3601 cgactgctgc gatcgcggcc aagcctcggg ccggaactcg aagtccggct gcaaaggctg
     3661 tccgagttgt ctccgccgcc cactcgcagc actcatcgac agtcgcgtta tgtgacggtc
     3721 gatgtcgaaa cgaccggact cgacatgcgg catgaccacg tgttgtcgat cggggcggtt
     3781 gcggtcgaac acggcgtgat cgcactcgcc cactgctttg aggtcgtgct gcggcaggcc
     3841 gaatcatcga gccacgacaa catcctggtc catcgcatcg gcggccagcg ccagcttggc
     3901 ggcgaagacc cggcagaatc gttcgtacgg ttcctggaat acgtcggcca ctgcccgctg
     3961 gtggccttcc gcgcggaatt cgaccagacc atgctcgacc gagccttgaa ggagcatctc
     4021 aacatcgtca gccagagcca atggctcgat ctggccgaac tgctgccggc gctctacccc
     4081 tccaacgaaa acaagaccat ggacgactgg ctggcggcga tgaacattca catgctggcg
     4141 cgccacgacg cgctcgccga cgcgctggcc acggcacaga tgctgcaggt ctgtttgcgg
     4201 caggccgaat cgctggaaat gacctgcccg gctcacctgc tggaaatgca gcgggcgcaa
     4261 cattggctcg gcaagcgcta gtcagcgacg cttaggcacc tcgcgactac tctggggcca
     4321 gttgcaactg agagaagcgc cctgcgggca gccccgtgca ggccgtgcgc agcctgcgca
     4381 ggcttcgaat cagccgttcg gccggccaca ggggcgatcc agatcaagtc ccgggcgcac
     4441 gggcggagcc tataattcgg cgctccaaca aggagttagg tgccatcgtt cggccaatgc
     4501 atctcctcac gctcggactt aatcacacga ccgcgccgct cgccgtgcgc gaacgggtgg
     4561 cgttcgtccc ggaggaagtg tgcacgacga ttggccggct gcgcgagaag ctgtcggagc
     4621 cctcgaacgg ccgtgtctcc gaagcggcga tcgtctcgac gtgcaatcgc accgagctgt
     4681 actgcgcggc ggaggagccc gaccgcgcgc agcgcgcact gcaggagttc gtcgccgcgg
     4741 aaaagcagtt gagcctggac gagctgcagc gtcataccta tgtgctgccg cacgcaagcg
     4801 cggtgcgcca tgcgttccgc gtggccagcg ggctcgattc catggtgctg ggcgagccgc
     4861 agatcctcgg ccagatgaag caggccgaga agcttgcgcg gacggcgggc gggctgggct
     4921 tgatgctgaa ccacctgttc cagcgcacgt ttgccgtggc aaaggaagtg cgcaccacga
     4981 ccgagatcgg cacgcagtcg gtgtccatgg ccgcggcggc cgtgcgcgtg gctcagcgta
     5041 tcttcggcaa cctctccggc gagcacatcc tgttcgtcgg ggccggtgaa atgatcgagc
     5101 tgaccgcgac gcacttcgcc gcgcagcatc cgaagtcgat cacggtggcc aaccgcacgc
     5161 aggaacgcgc gcaggcgctg gcgcaccgct tccatgccaa tgcgatcaag ctcgccgaac
     5221 tgccggacat gatctcgcgc ttcgacatcg tggtgtcgtg caccgccagc tcgctaccga
     5281 tcattggcct cggcatgatc gagcgcgcca tcaagtcacg gcggcgcaag ccgatgttca
     5341 tcgtcgacct ggccgtgccg cgcgatgtcg agccggaggt tgcgcgactc gacgacgtct
     5401 acgtgcatac cgtcgatgac ctgggccgca tcgtgcagag cggcagcgaa agccgccagg
     5461 cggccgtcgc ccaggccgag gcaatcatcg aatcccgcgt gcgcgacttc gaggagtggg
     5521 tcaaggcgcg ccgcgcggtg ccggtgatcc aggacctgcg cacgcgcgcc gacgcgctgc
     5581 ggcgccgcga actcgaacgc gccctcaagc agctggcgcg cggcgaggcg cccgaagccg
     5641 tcatcgagca cctgtcacag gcgctcacga acaagttcct gcacgatcca atgagcacgc
     5701 tgcgcgaagt cggtgacgac gccgaacagg cgcgcctcca ggcgctgctc gcgcgcttct
     5761 actcccagtc cgacgattag ctgccgcccg cgggcgcaac ggccgcgggc cgccgcgacg
     5821 ccgcgggcga ttccattgcc ggtccctcgt ccgggcgcag tgctgttcaa gttcattgaa
     5881 cccccggcag ctcggcgagc cgtccacccc agacacccga tttgcaggcc accatgaaga
     5941 cttcaatgca gaccaaactc cagcagcttg cccgccggct ggaggagatc gacgtgcagc
     6001 tgtcgagcga ggacgcggcg cgcgacatga accgctaccg tgccctcggc caggagcgct
     6061 ccgaaatcga gcccgtggtg acgcgcttcc acgaatacaa ccgggcgcac gcggacctgc
     6121 tgggcgccca ggagatgctg tccgatccgg acatgaagac gctggccgaa gaagagaccc
     6181 acgtcgcacg cggccgcatg gaatcgatcg aaagcgacct gcagcgactg ctgctgccgc
     6241 aggacccaaa cgacgatcgc aacatcttcc tcgaggtccg cgccggcacc ggcggcgacg
     6301 agtcggggct gttcgctggt gacctgctgc gcatgtacat gcgctttgcc gagcggcatc
     6361 gctggaccac tgagatgatc tccgagagtc catccgacct cggcggctac aaggaagcaa
     6421 tcgtgcgcat cgttggcacc ggtgcgtact cgaaactcaa gttcgagtcc ggcggccatc
     6481 gcgtgcagcg cgtgccggag accgaggcgc aggggcgcat ccatacctcg gcgtgcacgg
     6541 tggcggtgct gccggaggct gacgagatca acgagatccg gatcgatccc tccgagatcc
     6601 gcatcgacgt gtaccgggcc tccggcgcgg gcggccagca cgtcaacaag accgagagcg
     6661 cggtgcgcat cacgcacttg ccgaccggca tcgtggtcga gtgccaggat gatcgctcgc
     6721 agcacaagaa tcgcgaacgc gcaatgaaga tcctcgctgc gcgcatcaag gaccagcaga
     6781 cgcgcgagca gcaggccaag gaagcgtcgg cacgcaagag cctgatcggc tcgggcgatc
     6841 gctcggagcg catccgcacc tacaacttcc cgcagggtcg cgtgaccgac caccgcatca
     6901 acctgacgct gtacaagatc gaccagatca tggacggtga catcgacgaa ctgaccgagc
     6961 ggctggccgc ggagcaccag gccgaactgc ttgccgcgct tggcgagtcg gcctgattgc
     7021 gcactgccga cagtctgctg cgcagtgcgg acctgccccg caccgaggcg cgcgcactgc
     7081 tcgcgtcggc cctgcgagtc acgcgcgagc gactgatcgc ctttccggag accgaagtgt
     7141 cgccgaccgc gctggccgcc ttcgaggcac tggcggcgcg ccgacgcgaa ggcgagccgc
     7201 ttgcctacct gctgggcgag aaggagttct acggccgacg ttttgcggtg acacccgacg
     7261 tactcgtgcc gcgaccggat accgagtcgc tcgtcgaggt cgcactggaa tgcctgtcaa
     7321 acgtgatggc gcctcgcgtg ctcgaactcg gcaccggctc cggctgcatt gcggtcacgc
     7381 tgcaactcga acggcccgac gcacgcgtcg tggcaaccga tgtatcagcc gtcgccctgg
     7441 ccgtggcccg caccaatgcg caggacctca aggcccccgt aactttccgg aagggggact
     7501 ggtacggagc cgtcgattca gccgagccgt tcgatctgat cgtgagcaat ccgccatacg
     7561 tggccgctgc cgatccgcac ctcgacgcgc tgaagcacga accggcgatg gccttatcgg
     7621 atggccacga tggcctgcag tgcctgtcta ccctcatcgc aggtgcgcga gcgcacctgt
     7681 acgcgagtgc ctggctggtt ttggaacatg gcttcgatca gtccctggcg gtcgcgcggc
     7741 tgctccgcga agctggtttt cgggaggtgt cggtgcgatg cgatgccggc ggccagccgc
     7801 gggtgtccta tggccgctct tgaggtgccg cgggaggacg cctcggagaa ggacgcgatt
     7861 gatttggcga ccgtcagcga cggtcaaccg ggaatgcacg aaccgtcgtg catcggagga
     7921 atttggtgcg ccgaatttcg ggcgtgacgg ccgccagaac tgcggtgaca tctccgggtc
     7981 acggcgcaat ggcattggaa ccgcttgtgc tgcgggcagg tggttcgaaa atggccttcc
     8041 cccgccttga attcgacgcg acagcccatt caagttgcgc tgcgggagcc cggttcgtac
     8101 gtccacacgg ggtcaccccg gtttgacgga gatccaccca actttctaca ctgaccctcc
     8161 cttccgagat catcgccatg gacgccaagg aattcatcca aaagacagtc accgaccatc
     8221 cggtcgtgct cttcatgaag ggcacggccc agtttccgca gtgtggcttc tccggccgtg
     8281 ccatccaggt gctcaagcag gccggggcgc cgagcctcgt caccgtcaat gtgctcgacg
     8341 acgacgacgt gcgccagggc gtgaaggatt acgccaactg gccgacgatc ccgcagcttt
     8401 acgtgaatgg gcaattcgtc ggcggctcgg acatcatcat ggaaatgttc cagtcaggcg
     8461 agctgcagac gctgctgagt caggccaagg ccgcgtgatg gcggcgccgg cgcgccggct
     8521 gatcgttgcc atcactggcg ccagcggagc gatctacggc gtccgcctgc tgcggcgact
     8581 gcaggagcgt accgacgtcg agacccacct gctgctgtcg tcggccggcg tactcaacgt
     8641 ctaccaggaa atggacctga agcgcggcga tgtcgaggcg ctggcgcacg tcgtccacag
     8701 cgtcaacgat gtcggcgcga cgattgccag cggttcgttc gctaccgccg gcatggtgat
     8761 cgcaccgtgc tcgatgaaga cgctggcagc gatcgcgcac gcgtacgccg atacgctgat
     8821 cacgcgcgcc gccgatgtct gcctgaagga gcggcgacgc ctggtgctgc tgacacgcga
     8881 ggcgccgctc aacctggccc acatccgcaa catggaagcc gtcacgctga tgggcggcat
     8941 catctttccc ccggtacccg cgctgtacca ccgcccgacg tcgatcgacg agatggtcga
     9001 ccataccgtg agccgcgtgc tcgatctgtt cgatctggag gattcgaagt cgccgcgttg
     9061 gagcggcgcg ctcggcccca cttaagactg agtgcctagg ccgggtggtt aggcaaaggg
     9121 aagttgcgtc gtgccgttgt cagacgccgc tgccttcaag acgaggcagc acacaaagca
     9181 agagccccgc cgaagcgggg ctctttgaag ccaatgccga ctatctcctg cggcgacgtg
     9241 tcgccgcgat gccggcgaat ccgaggctga gaagcgcacg ggtaccgggc tctggtaccg
     9301 ggagtgactc gaacgcatga aactcggcgc gaacgaaact ctcgccctag aagaggctta
     9361 tgggtgcatc gaatgctccg ccaccgagga cgtttccgcc ctgcaaatag ctgtagctcg
     9421 cgaagaagtt ggtattggtc gtatccgggc gcgttaactg cagcaggatc tggtccgcac
     9481 cgtttggagg agcaaagtca gcgaggccac gttcgaaaat ctcatttgtg tcgaagtcct
     9541 gcagtaggta gcgcacctcg atcttgcccg ttaccggatt gaacctcacg tccagttcgg
     9601 ccgcctgctt cactccgtct gcatttcgat cggtgaaacg aatcccatag ccgttgatca
     9661 acggtccggt cagcgaactg agatcgaaga gtcccgtcaa accgagcgtg tcgtcgatct
     9721 tgagcccagt gccgaggtcc gacgagtctg tgttggtgtt cagagctgcg cgcaaattaa
     9781 ggcggtcctg cccatttgca ttttgggtca gtccaccgtc ggcggagttg agcctcaacc
     9841 gaccgtccga ttccgagccc acgacgaatg taccgaacac accgtagcta gccggttgac
     9901 cattcggaat cggacggtcg gttgaggctc aac
//
LOCUS       DATIRT010000314         9344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_737274, whole genome shotgun sequence.
ACCESSION   DATIRT010000314 DATIRT010000000
VERSION     DATIRT010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9344
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737274"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /gene="ppnK"
                     /locus_tag="VLN25_07200"
     CDS             <1..210
                     /gene="ppnK"
                     /locus_tag="VLN25_07200"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523404.1"
                     /note="catalyzes the phosphorylation of NAD to NADP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HSQ10385.1"
                     /translation="VEIEITDVRDASAHFDMQAYSSLVPGDIVRARRSHDLVTLLHPI
                     GYNFFATLRQKLHWSVMPSESHSRG"
     gene            232..1884
                     /gene="recN"
                     /locus_tag="VLN25_07205"
     CDS             232..1884
                     /gene="recN"
                     /locus_tag="VLN25_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HSQ10386.1"
                     /translation="MLVSLSIRNFVIVDELELEFAPGFTVLTGETGAGKSILIDALLL
                     ALGERGDADVVREGATRADIAAEFRPGPVVSRWLVDNELNGDDAGLLLLRRTIDANGR
                     SRAFVNGAAVTLAQLRDLGEQLVDVHGQHAHQSLLRPAAQLELLDDHGALGEARATVA
                     DAYADWKREQREREQAESMAASATAEQERLRWLVDELGTLAPEPGEWERVESEHKRLS
                     HGAGLLEGARAAVDALSESDAAALTQVAATGSRLTQLAAYDERLKPVIELIDGAQVQL
                     EEAVSALQRYADRSDLDASRLADVEARVTALHGAARRFRVAPEALAGLLAEARDKLAA
                     LSAAGDLDTLRGRESAARQRYDGAAARLSKARATASRRMSAEVSRAMQDLAMAGGKFE
                     VQLVESGPSAHGNEKAEFLVAAHAGVAVKPLARVASGGELARISLAIAVIAATATPVP
                     TLIFDEVDAGIGGAVAETVGRLLKQLGQSRQVLCVTHLPQVAARGDQHYAVTKELTAD
                     QRPMSQMTQLERRARVEELARMLGGQEITETTRKHAREMLSS"
     gene            complement(2128..3048)
                     /locus_tag="VLN25_07210"
     CDS             complement(2128..3048)
                     /locus_tag="VLN25_07210"
                     /inference="COORDINATES: protein motif:HMM:NF004793.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HSQ10387.1"
                     /translation="MRFFTAEDVHAALDFDRLADAIAAVLAAAPVAPLRHAHALNDMD
                     TLLLMPAWSAQALGVKLVTVMPGNRALGAATVNAIYVVFDRATGAAAAVIDGEALTLR
                     RTAATSLLAARHLARPDARNALIIGTGTLAPYMAHAHARACRLERLWIWGRRGEGAQS
                     LAQRLRDEGLPAQAVEDLEAAVRGADVISCATTSTQPIVRGAWISPGTHLDLVGGFRR
                     GMREADDEAIARARIVVDSYAGALAEAADIVEPLERGVIERSAIVAELAELVAGRPGR
                     TGASEITLFKSVGTAIADLAAAQLLLERAG"
     gene            complement(3048..3530)
                     /gene="moaC"
                     /locus_tag="VLN25_07215"
     CDS             complement(3048..3530)
                     /gene="moaC"
                     /locus_tag="VLN25_07215"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HSQ10388.1"
                     /translation="MKPLTHFDAAGQAHMVDVGDKPVTHRVAVATGRITMQPATLALV
                     QAGHAKKGDVLGVARIAAIQAAKRTSELIPLCHPIAITRIAVEFDIDAAAYSVECQVR
                     VETHGPTGVEMEALTAVQIGLLTIYDMCKAVDRGMIIGDVRLIEKHGGRSGSFVADPA
                     "
     gene            3699..5249
                     /locus_tag="VLN25_07220"
     CDS             3699..5249
                     /locus_tag="VLN25_07220"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016475160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HSQ10389.1"
                     /translation="MTRILHKLRFGTVLFVALALCAPVSLPARAQAVDLPTLGEAGAE
                     ELSPALERKIGEQIMLQIRRDPAYLPDTEATEYLNHLGYQLVSVSAARTMDFTFFLVR
                     DPMLNAFALPGGFIGVHSGLMIAAQNESELAGVLAHEIGHVSQRHIARMLGQQKDSLA
                     IQIGALLLAILASRAGGNSGGDLAQAAIMGGQAAAIQQQLNFSREAEREADRVGFVTL
                     VNANFDGRGIEQFFGRLQQGSRLYEGTAPAYLRTHPMTVERISDMQNRSRTLQFKQRA
                     DPIDFHLVRARLRVLQETGVQGWRDQLDYFNSQLKNRTAASEAAARYGAGVAALKLNM
                     PDLALENALAARRATTASSAILDKLVSETRFAAAKSDAERKEALEMARDGAARYPLST
                     VAVAHYIDLLNKSNLHQQAIDVLREQAAITRTQAGYYALLGRSYESLGKKSLHHQAIG
                     EMYALLGVKPAALQQLEQARRANDGDFYTMSEIDARVRELQSEIKREQEAARESGRGA
                     PPEARKSR"
     gene            5400..6485
                     /locus_tag="VLN25_07225"
     CDS             5400..6485
                     /locus_tag="VLN25_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSQ10390.1"
                     /translation="MSPPENQQLSTPVDATGAIETDAVIVGAGPVGLFQVFELGLLEI
                     KAHVIDSLKDMGGQCVELYPDKPIYDIPAVPVCTGKELTDSLLKQIEPFGATFHLGQE
                     VSVVQKQEDGRFYVETIKGTRFLTKVVIIAAGVGSFQPRTLKVPDIERFLGSQLHYRV
                     RDPAQFQGKNLVIAGGGDSALDWAIDLAGKAGSVVLVHRRDGFRAAPASVARMKDMCE
                     RHEMQFLVGQITGFEESAGRMTDLRVTSLDGLTRRVPLDALLVFFGLSPKLGPIADWG
                     LDLERKQIVVDTEKFETNVPGIFAVGDINLYPGKKKLILSGFHEAALAAFGATHYIFP
                     TRKIHLQYTTTSPKLHKILGVESPVFD"
     gene            complement(6532..7137)
                     /locus_tag="VLN25_07230"
     CDS             complement(6532..7137)
                     /locus_tag="VLN25_07230"
                     /inference="COORDINATES: protein motif:HMM:NF022609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HSQ10391.1"
                     /translation="MPMLRPMDASVIAALAKWPDVPAVFGWLSLTARGQWRLRGDPIG
                     NAAIREFIGRNYAGDDRGRWYFQNGPQRVYVSLELTPWVYRLEAAGSITTHTGLAPDR
                     CTGAALLDSDRVVLVTELGPGAIDDRDASRFLRDVSDAAGRTLDTRGCERWLRGTDDA
                     YLSPERLSLSGPAVRFERVTSDLLERRWKFVREPVEDADGS"
     gene            complement(7204..8181)
                     /locus_tag="VLN25_07235"
     CDS             complement(7204..8181)
                     /locus_tag="VLN25_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSQ10392.1"
                     /translation="MRGSAATQYVAPGWLPGGHLQTIVPARLVPLPRVDYRRERWDTP
                     DGDFVDLDFASPEPVEPMAPVLVLFHGLEGCSQSHYSRSIMRDAADRGWRGVVAHFRG
                     CSGVANRLPRAYHSGDSDEGDWILRRAHAHWPLAPLYAVGISLGGNMLAKWVGERAED
                     ARFVTAAASIGAPLDLAAGGAALARGFNLVYTKVFLASLKPKALAKVERFPGVAHRDR
                     LRASRNLYEFDNAYTAPVHGFRNTEDYWNRASGKPWLGGVRVPLLVLNALNDPFVPAD
                     SLPDLSGVSRDVRLEQPAEGGHIGFARGSLPGRLDFLPRRLHEFFTRGV"
     gene            complement(8178..8621)
                     /locus_tag="VLN25_07240"
     CDS             complement(8178..8621)
                     /locus_tag="VLN25_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HSQ10393.1"
                     /translation="MSRLTRPLLFASPEECEHAFYEALEAADVDAVVDLWLEDDDVVC
                     IHPNGPRLLGFAAVRASWSAILANGPLHVRGTLRKSLDTPTVAVHNVIEEVLVGQGTA
                     QQVVSVLATNVYVKTSAGWKMVLHHGSQASGGEVVSTPEPNAILH"
     gene            complement(8644..8865)
                     /locus_tag="VLN25_07245"
     CDS             complement(8644..8865)
                     /locus_tag="VLN25_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HSQ10394.1"
                     /translation="MSSAEPAVKTGRTAAPVVELVARDLPAFCPNPVMPVWNHHPRVF
                     LDVATTGEAMCPYCGTVYRLKAGEIVKGH"
     gene            complement(8862..>9344)
                     /locus_tag="VLN25_07250"
     CDS             complement(8862..>9344)
                     /locus_tag="VLN25_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HSQ10395.1"
                     /translation="FQRHHINVSMVRSKTAGHYVNSILANQEAVRDGYDEALLLDTQG
                     FVAEGAGENLFIVKDGQIIEPEMVSGLTGITRATVIELARDLGYTVHSRPMTRDDVYL
                     ADEAFFTGTAAEVTPIRELDGRVLGAGQRGPVTERIQSLFFDVVNGRAPKYSRWLTLV
                     "
BASE COUNT         1620 a         3159 c         3058 g         1507 t
ORIGIN      
        1 gtcgagatcg agatcaccga cgtgcgcgac gccagcgcgc acttcgacat gcaggcctac
       61 tcgagcctgg tcccgggcga catcgtgcgc gctcgccgca gccacgacct ggtcacgctg
      121 ttgcatccga tcggctacaa cttcttcgcc accctgcgcc agaaactgca ctggagcgtg
      181 atgccgagcg agtcgcactc gcgcggctga gcctcagccc gacgtcagag catgctggtc
      241 tcgctctcga tccgcaattt cgtgatcgtc gacgaactcg aactcgagtt cgcaccgggc
      301 ttcaccgtct tgaccggcga gaccggcgcc ggcaagtcga tcctgatcga cgcgctgctg
      361 ctggccctgg gtgagcgcgg cgacgccgat gtggtgcgcg agggcgccac gcgggccgat
      421 atcgcggccg agttcaggcc cggtcctgtc gtctcgcgct ggctcgtcga caacgaactg
      481 aatggcgacg atgccgggct gctgctgctt cggcgaacga tagatgcgaa cggccgcagc
      541 cgcgccttcg tcaatggcgc cgccgtcacg ctggcccagt tgcgcgatct tggcgaacaa
      601 ctggtcgacg tgcacggcca gcatgcgcac cagtcgctgt tgcgaccggc cgcgcagctc
      661 gaactgcttg acgaccacgg agccctcggc gaggcccgcg ccaccgttgc ggacgcctac
      721 gcggactgga agcgcgagca gcgtgaacgc gaacaggccg aatcgatggc tgccagcgcg
      781 accgcggaac aggaacgcct gcgctggctc gttgacgagc ttggcacgct cgcgcccgag
      841 cccggtgaat gggagcgcgt cgaatccgag cacaagcgcc tctcgcatgg cgcgggtctg
      901 ctcgagggag cgcgcgccgc ggtcgatgcc ctgagcgagt ctgatgctgc ggcgctcacc
      961 caggtcgcgg cgaccggcag ccgactgacg cagctcgccg cctatgacga gcgattgaag
     1021 ccggtgatcg aactgatcga cggcgcccag gtgcagctcg aagaggcggt cagcgctttg
     1081 cagcgttatg cggaccggtc cgacctcgat gcgtcgcggc tggccgatgt cgaagcccgc
     1141 gtcactgctt tgcacggtgc cgcgcgccgc tttcgcgtgg caccggaagc gctggccggc
     1201 ctgcttgcgg aagcacgcga caagcttgcc gcgctgtcgg ccgcgggcga tctcgacacg
     1261 ctgcgcgggc gcgagagcgc agcacgccag cgttatgacg gggccgcggc acgactgtcc
     1321 aaggcccgcg ccacggcgtc gcgccggatg tcggccgagg tcagtcgtgc gatgcaggac
     1381 ctggccatgg ccggcggaaa attcgaggtg cagctggtcg agtctggccc ctcggcccac
     1441 ggcaacgaga aggcggaatt cctggtcgcc gcgcacgccg gcgtggccgt gaagccgctt
     1501 gcgcgcgtcg cctccggcgg cgagctggcg cgcatctcgc ttgcgatcgc agtcattgca
     1561 gcgaccgcaa cgccggtgcc gacgctaatc tttgacgagg tcgacgccgg tatcggcggt
     1621 gctgtcgccg aaaccgttgg tcgcctgctc aaacagctcg gacagagtcg ccaggtgctg
     1681 tgcgtgacgc atctgccaca ggtggccgcg cgcggtgacc agcattacgc ggtcaccaag
     1741 gaactcaccg ccgaccagcg gccgatgtcg cagatgactc agctggagcg gcgcgctcgc
     1801 gtcgaggaac tggcgcgcat gctcggcggc caggagatca ccgagacgac gcgcaagcat
     1861 gcgcgcgaga tgctgagcag ctagcacccc tcgtgtacgc gaccggcttt gctgatcgca
     1921 cgtgcactcg gagtggcacg gctcgctgcg cgagccgctc aatacttgat aatcggctgc
     1981 gctttgggcg tagcggggaa ttgtcgacag cacctgcgtg ctgtcgaccc cgtgcagccg
     2041 gcgcggcctg catgccgcgc agtgggccgg ccgcgcgcta cgccgaaaca gaaaggcacg
     2101 caacacatcg cactgtgacg agccgcacta ccccgcccgt tccagcagca actgcgccgc
     2161 cgcaagatcg gcgatggcgg tgccgaccga cttgaaaagc gtgatctcgc tcgctccagt
     2221 acggccgggc cggcctgcca ccagttccgc cagttcggcc acgatcgccg aacgctcgat
     2281 gacaccccgc tcgagcggct cgacgatatc cgccgcttcg gccagcgccc ccgcatagct
     2341 atcgacgacg atgcgcgcac gcgcgatcgc ctcgtcgtcg gcctctcgca tcccgcggcg
     2401 gaatccgccc acgaggtcga ggtgcgtgcc ggggctgatc caggcacccc gcacgattgg
     2461 ctgggtcgat gtcgtcgcgc aactgatcac atccgcaccg cgaacagccg cctccaggtc
     2521 ctcgaccgcc tgcgccggca gtccctcgtc acgcagtcgc tgcgccaggc tctgggcacc
     2581 ttcgccgcgc cggccccaga tccacaatcg ctcgagcctg catgcccgcg cgtgcgcatg
     2641 tgccatgtac ggcgccagcg tgccggtacc gatgatcaac gcgttgcgtg cgtccggccg
     2701 cgccagatgg cgcgctgcga gcagcgatgt cgccgccgtg cgtcgcagcg tcaacgcctc
     2761 gccgtcgatc acggcggcag cggcgcctgt cgctcgatca aagacgacat agatggcatt
     2821 gacggtcgcc gccccgagcg cgcgattgcc gggcatgacc gtcaccagtt tcactcccag
     2881 cgcttgcgct gaccacgccg gcatcagcag caacgtgtcc atgtcgttca gcgcgtgggc
     2941 gtgacgcagg ggcgctaccg gcgccgccgc caggacggcg gcgatcgcat cggccagtcg
     3001 atcgaaatcg agtgccgcgt ggacgtcctc cgcggtgaaa aaacgcatca ggccggatcg
     3061 gcgacaaaac tgccggagcg gccgccgtgc ttctcaatca ggcggacgtc gccgatgatc
     3121 atgccgcgat cgactgcctt gcacatgtcg tagatcgtca gaaggccgat ctgcaccgcc
     3181 gtcagcgcct ccatctcgac gccggtcggg ccgtgcgttt ccacgcgcac ctggcattcg
     3241 acgctatagg cagcggcatc gatatcgaac tccacggcga tgcgcgtgat cgcgatcgga
     3301 tggcatagcg gaatcagttc cgacgtgcgc ttggctgcct gtatcgcagc gatgcgagcc
     3361 acgccaagca cgtcgccctt ttttgcatga cccgcctgca ccagggccag tgtcgccggc
     3421 tgcatcgtga tgcgtccggt cgccacagcc acgcgatggg tcacgggctt gtcacccacg
     3481 tcgaccatat gggcctgtcc ggcggcatca aaatgcgtca atggcttcat ctggcagcaa
     3541 cacttccaac agggaacgaa acacccgact ggctatcata cggacgatat gagaccgtct
     3601 gcgagcgtcg tccgtgagct taagtcgctc ctctgacctg aattgacctg gcgtgacccc
     3661 acgaaggtca cgggcaacct tcgccccgag ttcctgactt gacccggatc cttcacaaac
     3721 ttcgcttcgg caccgtactg ttcgttgccc ttgccctctg cgccccggtg tcactgccgg
     3781 cgcgcgcgca ggcggtcgac ctgccgacgc tgggcgaggc tggagccgaa gagttgtcgc
     3841 ccgccctcga gcgcaagatc ggcgagcaga tcatgctgca gatccggcgt gaccccgcct
     3901 acctgccaga taccgaagcg acggagtatc tcaaccacct cggctatcag ctcgtgtcgg
     3961 tcagcgcggc gcgcacgatg gacttcacgt tctttctggt gcgcgatccg atgctcaatg
     4021 ccttcgcgct gccgggtggc ttcatcggtg tccacagcgg tctgatgatc gctgcgcaga
     4081 acgagtccga actggccggg gtgctcgcac acgaaatcgg tcacgtttcg cagcgtcaca
     4141 tcgcccgcat gctgggacag cagaaggact cgctggccat ccagatcggc gcgctcctgc
     4201 tggccatact ggcctcgcgc gccggtggaa actcgggcgg cgaccttgca caggccgcca
     4261 tcatgggcgg ccaggcagcg gcaatccagc agcagctcaa cttctcgcgc gaggccgaac
     4321 gcgaggccga tcgggtcggc ttcgtcacgc tggtgaatgc caattttgat ggccgcggca
     4381 ttgagcagtt cttcgggcgc ctgcagcagg gctcgcgcct gtacgaaggc acggcacccg
     4441 cgtatctgcg tacgcacccg atgacggtcg agcggatctc cgacatgcag aaccgttcgc
     4501 gcacgctgca gttcaagcag cgagccgacc ccatcgattt ccatctggtc cgtgcccgac
     4561 tgcgcgtgct gcaggaaacc ggcgtgcagg gctggcggga tcagctcgat tacttcaaca
     4621 gtcagctgaa gaatcgcacc gcggcgtctg aggcggcggc ccgctatggc gcgggagtcg
     4681 ccgcgctcaa gctcaacatg cccgacctgg ccctggaaaa tgcgctcgcc gcgcgccgcg
     4741 ccaccacggc gtcatcggcc atcctcgaca agctcgtctc ggagacgcgc ttcgccgccg
     4801 ccaagtcgga cgccgagcgc aaggaagcgc ttgaaatggc gcgcgacggc gcggcgcgct
     4861 acccgttgtc cacggtcgca gtcgcccatt acatcgacct gctgaacaaa tcgaacctgc
     4921 accagcaggc cattgatgtc ctgcgagagc aggccgcgat tacccgcacg caagcggggt
     4981 actacgcgct gctgggccgc agctatgaat cgctgggcaa gaagtccctg caccaccagg
     5041 cgataggcga gatgtatgcg ctgctcgggg tcaagccggc ggcgctgcag cagctggagc
     5101 aggcccgtcg cgcgaacgat ggggattttt acacgatgtc ggagatcgat gcgcgcgtgc
     5161 gtgagctgca aagcgagatc aagcgcgaac aggaagccgc gcgcgaaagc ggccgtggcg
     5221 ccccgcccga agctcggaag tcgcgctgaa gagcggcttg atcgcccgac ctgccgggac
     5281 ggttcccggc cgcgggcccc cgaccgggaa tccgtacaat tgcgcgcccg cgacggactt
     5341 gcctgatccg atggcgccgc cggtcgcgct ggcaacggat gcaagccacg atgaactcac
     5401 tgtcgccccc cgagaaccag caattgagca cgcccgttga cgcgaccggc gccatcgaaa
     5461 ccgatgcagt gatcgtcggt gccggtccgg tcggcctgtt ccaggtgttc gaactcggcc
     5521 tgctggagat caaggctcac gtcatcgatt cgctgaaaga catgggcggg cagtgcgtcg
     5581 agctgtatcc ggacaagccc atttacgaca tcccggcggt cccggtgtgc accggcaagg
     5641 aactcacgga cagcctcctc aagcagatcg agccgttcgg tgcgaccttt catctggggc
     5701 aggaagtcag tgtcgtgcag aagcaggagg acgggcgctt ctacgtcgag accatcaagg
     5761 gcacgcgctt tctcaccaag gtcgtgatca tcgcggccgg cgtgggttcg ttccagccgc
     5821 gcacgctgaa ggtgcctgac atcgagcggt ttctcggttc gcagttgcat taccgcgtca
     5881 gggatccggc gcagtttcag ggcaagaacc tcgtcattgc cggtggcggc gactcggcgc
     5941 tcgactgggc gatcgacctc gcaggcaagg ctggcagcgt agtactcgtg caccggcgcg
     6001 acggcttccg cgccgctccg gcgtcggtcg cccggatgaa ggacatgtgc gagcgccacg
     6061 agatgcagtt cctggtcggg cagatcactg gcttcgagga gtctgccggg cgaatgacgg
     6121 atctgcgcgt cacgtcgctc gacggactga cacgccgcgt gccgctcgac gccttgctgg
     6181 tgttcttcgg cctgtcaccc aagctcggac cgatcgccga ctggggcctc gacctggagc
     6241 gcaagcagat cgtggtggat accgagaagt tcgagaccaa cgttccgggc atcttcgccg
     6301 tcggcgacat caacctttat ccgggaaaga agaagctgat cctgtccggc ttccacgaag
     6361 ccgcactcgc tgccttcggc gcgacccact acatcttccc gacccgcaag atccacctgc
     6421 agtacacgac caccagcccg aagctgcaca agatcctcgg cgtcgagtcg ccggtgtttg
     6481 actgaacccg gagattcgtt cgagagtcag cgcgccgtcg agtccgccga tctaggagcc
     6541 gtccgcgtct tcgaccggtt cgcgcacgaa cttccagcgg cgctccagca ggtcggacgt
     6601 gacgcgctcg aatcgcaccg caggtccact tagcgacagt cgctctggcg acaggtaggc
     6661 gtcgtcagtg ccgcgcaacc aacgctcgca gccgcgcgtg tcaagggttc ggccagccgc
     6721 atcgctcaca tcgcgcagga atctggacgc gtcgcgatca tcgatggcgc cgggtcccag
     6781 ttcagtgacc agcacgaccc ggtcactgtc gagcaatgcc gcgcccgtgc agcgatctgg
     6841 cgctaagcct gtgtgggtcg tgatcgatcc cgccgcctcc agtcggtaga cccatggcgt
     6901 cagttccaaa gaaacgtaga cccgctgcgg tccgttctgg aagtaccagc gtccacgatc
     6961 atcgccagcg tagttgcgac cgatgaactc gcggatcgct gcattgccga tcggatcgcc
     7021 ccgcaggcgc cattgcccgc gcgccgtcag cgagagccag ccaaagacag ccggcacatc
     7081 cggccacttg gcgagtgcgg cgatgacact tgcgtccatc ggccgaagca taggcatggt
     7141 cgaggtcgtc cggttcggcc gggattgcaa cttgcggtcg cggtgaagcg accgcggcag
     7201 agatcaaacg ccgcgcgtga agaactcgtg cagccggcgc ggcaggaagt cgagccggcc
     7261 cgggagtgac ccgcgcgcga agccgatgtg gccaccttcg gccggctgct cgagccggac
     7321 atcgcgcgag accccggaaa gatccggcaa gctgtcggcc ggcacgaagg ggtcgttcag
     7381 cgcattgagc accagtagag gcacgcgcac gccgcccagc cagggcttgc cggaggcgcg
     7441 attccagtag tcctcggtat ttcggaaacc gtgcaccggc gcggtgtagg cgttgtcgaa
     7501 ctcgtacagg ttgcggctcg cgcgcaggcg gtcccgatgc gccacaccgg ggaatcgttc
     7561 caccttggcc agcgccttgg gcttgagcga agcgagaaac acttttgtgt agacgagatt
     7621 gaatccgcgc gccagcgccg cgccaccggc ggcgaggtcg agaggcgcgc cgatcgatgc
     7681 ggcggcggtg acgaaacgcg catcctcggc ccgctcccca acccacttgg ccagcatgtt
     7741 tccgccgagc gagattccga cggcatagag cggagccagc ggccagtgcg cgtgcgcccg
     7801 acgcaggatc cagtcgccct cgtcggagtc gcccgagtga taggcccgcg gcagccggtt
     7861 cgctacaccc gagcagccgc ggaagtgtgc aaccacgccg cgccagccgc ggtccgcggc
     7921 gtcgcgcatg atcgaacgcg agtagtggct ctgagagcag ccttcaagtc cgtggaacag
     7981 cacgagcacc ggtgccattg gctcaacggg ttccgggctg gcgaagtcga ggtcgacgaa
     8041 atccccgtcg ggcgtgtccc agcgctcgcg gcgatagtcg acgcgcggca gtggcaccag
     8101 gcgcgccggg acgatggtct gcaagtgacc gcccggcagc cagccgggcg cgacgtactg
     8161 cgtggcagcg gagcccctca atgcagtatc gcgttcggct ccggcgtgct gaccacttcg
     8221 ccgccactgg cctgcgatcc gtgatgcagc accatcttcc agccggccga tgtcttgacg
     8281 tacacgttgg tggccaggac gctgacgacc tgctgggcgg tgccctggcc gacgaggacc
     8341 tcctcgatca cgttgtggac ggcgaccgtc ggcgtgtcta gcgacttgcg caaggtgccg
     8401 cgcacatgca gcggtccgtt ggccagtatc gccgaccacg atgcgcgcac cgcggcgaaa
     8461 ccgagcaggc gcggcccgtt cggatggata cacacgacat catcatcctc gagccagaga
     8521 tcgacgacgg catcgacgtc ggccgcttcg agcgcctcgt agaaggcgtg ctcgcattcc
     8581 tcgggcgaag cgaacagcag tggcctggtc aggcgggaca tgacggtccg gtcgcggcga
     8641 cggtcagtga cccttgacga tctcgcccgc cttcaggcga tacaccgtgc cgcagtacgg
     8701 gcacatcgcc tcgccggtcg tggcgacgtc aaggaacacg cgcgggtgat ggttccagac
     8761 cggcatcacc ggattggggc agaacgccgg cagatcgcgc gccaccagtt cgacgaccgg
     8821 cgccgccgtg cggccggtct tcacggcggg ttcggcggaa ctcatacgag cgtcagccag
     8881 cggctgtact tcggtgcgcg accgttcacc acgtcaaaga acagcgactg gattcgctcg
     8941 gtcaccggac cacgctgtcc ggctccgaga acgcgtccat cgagttcgcg gatcggcgtg
     9001 acctcggcgg ccgtgccggt gaagaacgct tcgtcggcca ggtagacgtc gtcgcgcgtc
     9061 atcggacgtg aatgaaccgt atagccgagg tcgcgcgcca gttcgatgac tgtcgcgcgt
     9121 gtgatgccgg tcaggccgga caccatctcg ggctcgatga tctgaccgtc cttgacgatg
     9181 aagagattct cgccggcccc ttcggccaca aagccctgcg tgtcgagcag cagcgcctcg
     9241 tcgtaaccat cgcgcaccgc ttcctggttg gcgagtatcg aattcacgta gtgaccggcc
     9301 gtcttgctgc gaaccatgga gacgttgatg tgatgacgct ggaa
//
LOCUS       DATIRT010000315         4683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1228974, whole genome shotgun sequence.
ACCESSION   DATIRT010000315 DATIRT010000000
VERSION     DATIRT010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228974"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VLN25_07255"
     CDS             <1..476
                     /locus_tag="VLN25_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10396.1"
                     /translation="RALITSRAETHEAVLIALGVAERTIRCLHRDLAVFDMGSVAVEE
                     RLHRLLLSHRSARVRLLVDEAAWLESRAPRLRALQRQFPHALEMRVAASDDPVGDDAC
                     LLADDHAALELKPTSVARGDLWVHNTPHVQPLAAAFERRWNAGGHNLAVVPLGLG"
     gene            643..966
                     /locus_tag="VLN25_07260"
     CDS             643..966
                     /locus_tag="VLN25_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10397.1"
                     /translation="MNKSLLIASMMVVALAACGKKEAPAPAPTPAPAAAPAPAPAPAP
                     APAADAAKPADAAAAPATPAGAPMTTAAPVGESGTSPMPGQDAAKAEAAAAVEKAKQA
                     AMPKK"
     gene            complement(1048..1821)
                     /locus_tag="VLN25_07265"
     CDS             complement(1048..1821)
                     /locus_tag="VLN25_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSQ10398.1"
                     /translation="MRICSLGSGSSGNGLVVEVREGPHASRILVDNGFNLRQLCQRLQ
                     RAGLEYESLDAVFVTHEHSDHVGGVGALARRHGLPVFCTAGTAAASMFDDGRIDWRRI
                     EAGQVVEIGALRVEPYAVQHDAAEPVQYTFTDGSRRAGLLTDAGECSDTIVAALSGLH
                     ALVLECNHDAEMLQRGAYPPYLKARIAGARGHLSNAQAADILDRIDRAQLGWIAAAHL
                     SRSNNRPDLARQALAGVLNCAEEEIGVAYQDVGLDWRAV"
     gene            complement(1835..3040)
                     /gene="bamC"
                     /locus_tag="VLN25_07270"
     CDS             complement(1835..3040)
                     /gene="bamC"
                     /locus_tag="VLN25_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HSQ10399.1"
                     /translation="MMFRTLLLRLLLSALVALSAGCTWTGSLFSDKIDYQNAKTRSAP
                     LEVPPDLSQLPREERFTVPERPQSFTASGTAAAGRPGAPAGSVTTAVGGTVAPTGALA
                     RLERQGDHRWLVVNMPPEKAWPVLLDFWPSVGLAVEKSDPAAGVMETVWAENKAKLDQ
                     GIIRQTLGRLLGSVYSTGEQDRYRARVERTEKGMSEIFISHRGMVEVFTSTAQDRTAW
                     QPRPSDPELEAEMLQRLLVRFDSSAAKAGPAVTAAAGSAAGAAAGTAATAGAAAGAPE
                     RAFIVKGSDGRSERLDIDDPFDRAWRRIGLALDRGGFTVEDRDRAKGIYFVRYLDPDF
                     EAKAKEEQGFFTKLFGREKPVPAPQYRVQLASAGDAGTQVRVFDSEGKPERSPAGDRI
                     LGLLKDQIR"
     gene            complement(3059..3934)
                     /gene="dapA"
                     /locus_tag="VLN25_07275"
     CDS             complement(3059..3934)
                     /gene="dapA"
                     /locus_tag="VLN25_07275"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HSQ10400.1"
                     /translation="MIKGSIVAIVSPMREDGALDFEAYRRLIEWHVAEGTNAIVAVGT
                     TGESPTVDPDEHGELIRVAVEAVKGHIPVIAGTGGNSTREAIELTRHAKAVGAAATLQ
                     VVPYYNKPTQEGLYRHFRQIAETVDLPVILYNVPGRTVADLANDTTLRLSDVPGIVGI
                     KDATGDLARASDLLKRLPAHFAVYSGNDDTALALMLLGGHGVISVTANIAPKLMSDLA
                     KSALAGDLARARQINNQLLPLHLKLFVEPNPIPVKWALAKMGRIPGGIRLPMTPLSMP
                     AEPVVLAAMKDAGLL"
     gene            complement(4020..4601)
                     /locus_tag="VLN25_07280"
     CDS             complement(4020..4601)
                     /locus_tag="VLN25_07280"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSQ10401.1"
                     /translation="MLPDEARPTSNPLARAPSPWVVRFAARLAPGARVLDVASGHGRH
                     AHHLAERGCLVTAVDVDATCATSLSGTPGVQFLHADLENEPWPFADQSYDAVVVVHYL
                     HRPLLPRLAAALAPGGLLIYETFARGNEQFGRPRNPDFLLRARELLDAFGDLRILAFE
                     DGYVAEPQPAMIQRLAAVRIEPRAYGPVESLAL"
BASE COUNT          783 a         1621 c         1519 g          760 t
ORIGIN      
        1 aacgcgcgct gatcacgtcg cgtgcggaaa cgcatgaggc cgtcctgatt gcgctgggtg
       61 ttgctgaacg cacgatacgg tgcctgcacc gcgatctggc cgtgttcgat atgggcagcg
      121 tcgcagtgga agagcggctg caccggctgc tgctctcgca tcgctcggcg cgcgtgcggc
      181 tgcttgtcga cgaagcggcc tggctcgaat cgcgcgcacc gcggttgcgg gcgctccaac
      241 gccagtttcc gcatgcgctg gaaatgcgcg tcgccgcctc ggacgatccg gtcggcgacg
      301 acgcctgcct gctggctgac gatcacgcgg cgctcgagct caagccgacc agcgtcgcgc
      361 gcggtgacct gtgggtgcac aacacgccgc acgtccagcc cctggcagcc gcgttcgaac
      421 gtcgctggaa tgccggcgga cacaacctgg ccgtcgttcc gctgggcctc ggctgagcga
      481 ctcgggtcgc tgccactcgc ccgcacttgc gcgaacgcct ataatgctgg cccgggtaga
      541 tcgatggcac gacggtcgaa accgatcgaa atcgcatcgt tgcctgcgcc catggcgtcg
      601 caccgctcac aagaattcat tcaccactca ggaaggttgc acatgaacaa gtcgttactg
      661 atcgcttcga tgatggttgt cgcgctggcg gcctgtggca agaaagaagc gcccgccccg
      721 gccccgacgc ccgccccggc cgccgctcct gcgcccgctc ccgccccggc tccggcacct
      781 gctgccgacg ctgccaagcc ggccgacgca gccgcagccc ccgccacacc cgcgggcgcg
      841 ccgatgacga cggcagctcc ggtgggcgag tcgggcacgt cgccgatgcc gggccaggac
      901 gccgccaagg cggaagccgc cgctgcggtc gaaaaggcca agcaagctgc catgccgaag
      961 aagtaattct cgcttcagcg ggaacgcaag ggccggcact gccggccctt gttatttctg
     1021 cgacgactgc tgcaactcgc gcagcactca gaccgcgcgc cagtccagcc cgacgtcctg
     1081 gtaggcgacg ccgatttctt cttccgcgca attcagcacg cccgcaagtg cctgccgcgc
     1141 gaggtccggt cggttgttgc tgcgcgacag atgcgccgcg gcaatccagc ccagctgcgc
     1201 gcggtcgatg cgatccagga tatctgcggc ctgcgcattt gacagatgtc cgcgggcgcc
     1261 ggcgatgcgc gccttcaggt agggcggata cgcgccccgc tgcaacatct cggcatcgtg
     1321 attgcattcg agaaccagcg catgcaggcc ggagagcgca gcgacaatcg tgtcggaaca
     1381 ttcgcccgcg tccgtcagca gcccggcgcg gcgcgaaccg tcggtgaagg tgtactggac
     1441 cggctcggcc gcatcgtgct gtaccgcgta gggctcgacc ctcaacgcac cgatctcgac
     1501 gacctgccct gcttcgatcc gccgccagtc gatacgcccg tcgtcgaaca tcgatgcggc
     1561 cgcggtgccg gcagtgcaga aaaccggaag tccatgacgg cgcgcaaggg ccccgacgcc
     1621 gccgacatga tccgagtgct cgtgtgtcac gaagaccgca tcgagcgatt catactcgag
     1681 gcctgcccgc tgcagccgct ggcagagttg gcgcaaattg aaaccgttgt cgaccaggat
     1741 gcggcttgcg tgcggaccct cccgcacttc gacgaccagg ccgttgccgc tgctgcccga
     1801 gccaagactg caaatgcgca gcacgtgcag ccgcttagcg aatctggtct ttgagcagtc
     1861 cgaggatgcg gtcgcccgcg ggagaacgct cgggcttgcc ctcgctgtcg aacacccgca
     1921 cctgcgtgcc cgcatcaccg gccgaagcaa gctggacgcg atactgcggt gcaggcaccg
     1981 gcttttccct gccgaacagc ttggtgaaga atccctgttc ttccttcgcc ttggcctcga
     2041 agtccgggtc gagatagcgc acgaagtaga tgcccttggc acggtcccgg tcctcgaccg
     2101 tgaatccgcc gcgatcgagc gccagtccga tgcgacgcca ggcacgatcg aacggatcgt
     2161 cgatgtcgag acgttcgctg cggccatcgg aacccttgac gatgaatgcc cgctctggcg
     2221 ccccagcagc agcgccagcg gtggctgccg taccggctgc cgcaccggcg gccgaccccg
     2281 cggcagccgt gacggccgga cctgctttcg cggccgagct gtcgaaacgc accagcagtc
     2341 gctgcagcat ctccgcctcg agctcggggt ccgacggccg cggttgccac gccgtccggt
     2401 cctgcgctgt cgacgtgaac acttcgacca tgccgcgatg actgatgaag atttccgaca
     2461 tgcccttttc cgtgcgctcg acgcgtgcgc ggtaccgatc ctgttcgccg gtcgagtaga
     2521 cggagccgag aagccggcca agcgtctgcc ggatgattcc ctggtcgagc ttggccttgt
     2581 tttcggccca gacggtttcc atcacgccgg cggccgggtc cgatttctcg acggccagtc
     2641 cgacgctggg ccagaagtcg agcaggacgg gccaggcctt ctccggcggc atattgacga
     2701 caagccagcg atggtctccc tggcgctcga gtcgcgccaa cgctcccgtc ggcgccaccg
     2761 tcccgcctac agcggttgtt acgctccccg cgggcgcgcc tgggcgcccc gccgcggccg
     2821 tccccgaagc cgtgaacgac tgcggccgct cgggcaccgt gaatcgctct tcgcggggca
     2881 gctgggaaag gtccggtggt acctccagcg gcgccgagcg cgtcttggcg ttctgatagt
     2941 cgatcttgtc cgagaacaga ctgccggtcc aggtgcaacc ggcggacagc gccacaagag
     3001 cactcagcag cagacgcaac aacaacgtgc gaaacatcat caagactcct gcaggcgcct
     3061 acagcaggcc cgcgtctttc attgcggcca gcacgaccgg ctctgccggc atcgacaggg
     3121 gcgtcatggg cagacggatg ccacccggaa tgcgacccat tttcgcaagc gcccatttga
     3181 ccggaatcgg gttgggttcg acgaagagct tcaggtgcaa cggcagcaac tgattgttga
     3241 tctgccgcgc ccgtgccagg tcacctgcca gcgccgactt cgcaagatcg ctcatcagct
     3301 tcggtgcgat gttggcagtg accgaaatca cgccatgccc gccgagcagc atcagcgcaa
     3361 gcgccgtgtc gtcattgccg ctgtagacgg caaagtgcgc aggcagccgc ttcagcaggt
     3421 cgctggctcg ggcaaggtca cccgtggcgt ccttgatgcc gacgatgccc ggcacgtcgg
     3481 acagacgcaa ggtcgtgtcg ttggcgagat cggcgacagt gcggcccggc acgttgtaga
     3541 ggatcaccgg caggtcgacc gtctccgcga tctggcgaaa atgccggtaa aggccttcct
     3601 gggtcggctt gttgtagtac ggcaccacct gcaacgttgc cgctgctccc accgccttgg
     3661 catggcgagt cagctcgatc gcctcgcgcg tggagttgcc gccggtaccg gcgatcaccg
     3721 gaatgtggcc cttcactgct tcgacggcga cacggatcag ctcgccgtgc tcgtccggat
     3781 ccacggtcgg cgactcgcct gtggtaccga cggccacgat cgcgttcgtg ccctcggcca
     3841 cgtgccactc gatcagccgc cgataggcct cgaagtcgag tgcgccgtct tcccgcatgg
     3901 gcgaaacgat ggcgacgatg ctgcccttga tcatggtgtc tgcggtctcc ctggccgaag
     3961 cacggaattc taccgtgcag cgctcggccg ccggcccaca ccgcgttcgg ccctgcacac
     4021 tacagcgcga gcgattcgac cgggccgtag gcccgcggtt caattcgcac ggcggccagt
     4081 cgctggatca tggctggctg tggctcggcg acgtaaccat cctcgaaggc gaggatgcgc
     4141 aaatcgccga acgcgtcgag cagctctcgg gcacgcagca ggaaatcggg gttgcgcggc
     4201 cggccgaact gctcgttgcc gcgtgcgaag gtctcataga tcaacaggcc gcccggggcc
     4261 agcgccgccg ccagtcgagg cagcaggggc cggtgaagat agtgcacgac gacgaccgcg
     4321 tcgtagctct ggtcagcgaa cggccaaggc tcgttctcga gatcggcgtg caggaactga
     4381 actcctggcg tcccggaaag cgacgttgcg cacgtcgcgt cgacatccac cgccgtgacg
     4441 aggcagccgc gctcagccag atggtgcgca tggcgcccat ggccacttgc aacgtcgagg
     4501 acacgggcgc ccggcgccag gcgtgccgcg aagcgcacga cccagggcga cggcgcgcgc
     4561 gcaagcgggt tggaagtcgg ccgagcctcg tccggcagca acgcacttgc ctctcgccta
     4621 atcgtaggta agcccaatgg cgctgcggac ctcgcgcatg gtgtcctgcg ccgtcgcgcg
     4681 ggc
//
LOCUS       DATIRT010000316         2371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_146402, whole genome shotgun sequence.
ACCESSION   DATIRT010000316 DATIRT010000000
VERSION     DATIRT010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146402"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..2006
                     /locus_tag="VLN25_07285"
     CDS             261..2006
                     /locus_tag="VLN25_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HSQ10402.1"
                     /translation="MNVLPAFKVRSLLAAAAAALTLSACGGGGGEAAAPVPAPATAVP
                     PLVIPEPPAGGFAPSGPTAAPAAPSDAQAVRFLAQSTFGATSAEIDRLKRLGYNDWIE
                     QQFQIPRSSHLDFVLGVYPIPTPEGVTVTIDPLYQSFWRQAITAPDPLRQRVTYALSQ
                     ILVTSAADAALQTAPHTLASYLDTLSIHAFGSYRDLLEAVSKHPAMGQYLTSLANRGD
                     GGRVPDENYAREVMQLFSIGLVQLNIDGTPKLVNGEPVDTYSMDDIRGLARVFTGWGW
                     GNTGTPDPVNGRFGGNNPQDPMRRVIPMQFYAQYHSPLEKQFLGITVPAGTAGPQALK
                     TALDALFSHPNVGPFIATRLIQHMVISNPSPAYVGRVAAVFNDNGKGVRGDLKATVRA
                     VLFDPEAVTPVAGPSEGKLREPTLRLAAWIRAFGATSASGNFRIGVLDNQLSQTPMRA
                     PSVFNYFRPGFVPPNTSLATAELVAPEFQITHEISVAAYANFMQGVIQNGVGTGSDVR
                     ADYTSTYALADNADALVARINLMLAANSLSTATVTSIRDAVNAIPMTAGNARQNRTQL
                     AIYLTMTSPEFLVQK"
     gene            2023..>2371
                     /locus_tag="VLN25_07290"
     CDS             2023..>2371
                     /locus_tag="VLN25_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal protein"
                     /protein_id="HSQ10403.1"
                     /translation="MTTQASRREFLRTASRLSVAGVATPWAVNLAAIGAAAAQTAPTD
                     YKALVCVFMYGGNDHSNMVVPFDNVSYTLYAQSRTNLTLAQTSLLPLATPTNPSMAGR
                     QVSLRSELQPLKSI"
BASE COUNT          425 a          806 c          738 g          402 t
ORIGIN      
        1 ctgggcggcg cgggtcgctt gcgatctggc cggtggaacc gctgccaaca ggaggccgag
       61 gatggcaacg cactgcagta acggcagcac tgacaaggac ttcctctctg gcgaagcgtt
      121 caatcggtcg gcgcagttgc aggagctagt tcattcgtcc gatagaccca tccaggcgcg
      181 gtcgctagcc tcccacaggg gtgaaccgcg acgtcgcggt gtctcatggc gcgcctgacg
      241 cctactgttc cggaaacccg atgaacgtgt tgcccgcttt caaggttcga agcttactgg
      301 ctgctgctgc ggccgcactg acactgtcgg catgcggtgg cggcggcgga gaggctgccg
      361 ctccggtacc cgcgccggcg acagccgtgc caccgctcgt cataccggag cctcccgcgg
      421 ggggcttcgc accgtccggc ccgactgccg cgcccgcggc accgtcggat gcgcaggcgg
      481 tgcgcttcct ggcgcagtcc accttcggcg caacgagtgc agagatcgac cggctgaagc
      541 ggcttggcta caacgactgg atcgaacagc agttccagat cccgcggtcc tcgcacctcg
      601 acttcgttct gggcgtgtat ccgatcccga cgccggaagg ggtcacggtc accatcgatc
      661 cgctgtacca gtcgttctgg aggcaggcca tcaccgcgcc tgatccgttg aggcagcgtg
      721 tcacctacgc gttgtcgcag atcctcgtca cgtcggcggc ggatgcggcc ctgcagacgg
      781 cgccccacac cctcgcgtcc tatctcgaca ccctttcgat ccacgcgttc ggcagctacc
      841 gcgatctgct cgaggccgtc tccaagcatc cggcgatggg tcagtacctg acctccctgg
      901 cgaaccgggg tgatggcggg cgcgtgccgg acgaaaacta tgcgcgcgag gtgatgcagc
      961 ttttctcgat cggactggtt cagctgaaca tcgacggcac gccaaagctg gtcaacggtg
     1021 aaccggtcga cacctacagc atggacgaca tccgcggact ggcgcgcgtc ttcaccggct
     1081 ggggttgggg caacaccggc actccggacc cggtcaacgg gcgctttgga ggaaacaatc
     1141 cgcaggaccc gatgcgccgc gtgataccga tgcagttcta cgcgcaatac cactcaccgc
     1201 ttgaaaagca gttcctcggc atcacggttc ccgccggaac ggccggtccg caggcgctga
     1261 agacggcgct cgatgcgctg ttcagtcacc ccaatgtcgg gccgttcatc gcgacgcgcc
     1321 tgatccagca catggtcatc agcaacccga gccccgccta tgtcgggcgt gtggcggcag
     1381 tgttcaacga caacggcaag ggcgtgcgcg gtgacctgaa ggcgaccgtg cgcgcggtgc
     1441 ttttcgatcc ggaggcggtg actccggttg ccgggccgag cgagggcaag ctgcgcgaac
     1501 cgacactgcg gctggccgcg tggattcgtg cattcggcgc cacctccgcg agcggcaact
     1561 tccgcatcgg ggtcctcgac aaccagctgt cgcagacgcc gatgcgggcg cccagcgtgt
     1621 tcaactattt ccggcccggt ttcgtcccgc ccaacaccag cctggctacc gccgaactcg
     1681 tcgcgcccga attccagatc acgcatgaaa tctcggtggc ggcctacgcg aactttatgc
     1741 agggcgtgat ccagaacggt gtcggcaccg gcagtgacgt gagagcggac tacacgtcga
     1801 cctatgcact ggctgacaac gccgatgcgc tcgtggcgcg aatcaacctc atgctggcgg
     1861 cgaattcact ctcgaccgcg accgtcactt ccatccgcga cgcggtcaac gcgatcccca
     1921 tgacggccgg caacgcccgc cagaaccgga cgcagctcgc gatctacctg acgatgacgt
     1981 caccggaatt cctggtgcag aaataagcgg acggacaaac cgatgacgac ccaagcttcc
     2041 cgccgcgaat tcctccgcac ggcttcacga ctgtcggtcg cgggcgtggc gacaccctgg
     2101 gcggtcaacc tcgccgcgat cggtgccgca gcagcgcaga cggcaccgac cgactacaag
     2161 gcgttggtct gcgtcttcat gtacggcggc aatgatcact cgaacatggt ggtgccattc
     2221 gacaatgtca gctacacgct gtatgcgcag tcgcgtacca acctgacact ggcgcaaaca
     2281 tcgctgctac cgcttgccac gccgaccaac ccgtcgatgg cagggcgcca ggtctcgctg
     2341 cggtccgaac tgcagccgct gaagtcgatc t
//
LOCUS       DATIRT010000317         1519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_540164, whole genome shotgun sequence.
ACCESSION   DATIRT010000317 DATIRT010000000
VERSION     DATIRT010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540164"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1242
                     /locus_tag="VLN25_07295"
     CDS             <1..1242
                     /locus_tag="VLN25_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HSQ10404.1"
                     /translation="GHCRKFDAGTMCPSFRVTRDEEHGTRGRANTLRLALSGQLGPEF
                     ASEDVRAALDLCVSCKGCKRDCPTGVDMAKMKIEFAWHWQKKHGLSLKDRIVAHLPRW
                     APWAARFPWVANLRDTLPGAARLSEKVAGLSAQRKLPRWRSDTFLTSAAARQTLDGAA
                     DVVLLVDTFSNYHEPENAHAALRVLKAAGYRVAVAHATRTDAEPARPLCCGRTYLAAG
                     LVDDAKREAQRMVDALAPHVAAGATVVGLEPSCLLSLRDEFLVMGLGDSAVQLAPGAL
                     MIEEFLAREHKEGRLRLPLQALPQKQALVHGHCHQKAFDVLNPVVTMLRWVPELQVEV
                     IESSCCGMAGSFGYEAAHYGVSMKMAELSLLPAVRNAEAGTLIIADGTSCRHQIADGT
                     HAAREAVHAIRVLASALAPGN"
     gene            complement(1239..>1519)
                     /locus_tag="VLN25_07300"
     CDS             complement(1239..>1519)
                     /locus_tag="VLN25_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HSQ10405.1"
                     /translation="TMTLDDTEALLRDVAYARRLGFGGKLAVHPRQVAAINEGFSPSP
                     ADLEWARRVVAAATEAGDNALRLDGKLVDRPVIERARALLRTPERSPR"
BASE COUNT          242 a          484 c          547 g          246 t
ORIGIN      
        1 ggccattgtc gaaagttcga cgcggggacg atgtgcccga gcttccgtgt gacgcgcgac
       61 gaagagcacg gcacgcgcgg ccgcgccaac acgctgcggc tcgcgctgtc cggccagctc
      121 ggacccgagt tcgccagcga agacgtacgc gcagcacttg acctgtgcgt gagttgcaag
      181 ggctgcaagc gtgactgccc gaccggcgtc gacatggcga agatgaaaat cgagtttgcc
      241 tggcactggc aaaagaagca cgggctgtcg ttgaaggaca ggatcgtcgc ccacctgccg
      301 cgttgggcgc cgtgggccgc acgcttcccg tgggttgcga acctgcgcga tacgttgccc
      361 ggtgcggcac ggctctcgga aaaggtcgca ggcctctcgg cgcagcggaa gctgccgcgt
      421 tggcgcagtg acacgttcct gacgagtgcg gctgcgcgcc agactttgga cggcgcggcc
      481 gatgtggttc tgttggtcga cacgttcagc aactaccacg agcccgagaa cgcacacgca
      541 gcgctgcgcg tactgaaggc cgcgggctat cgggtggccg tggcgcacgc gacgcgcacc
      601 gatgcagagc ccgcgcggcc gttgtgctgt ggacgcacct atctggcggc gggacttgtc
      661 gacgacgcca agcgtgaagc gcagcggatg gtcgacgcac tcgctccgca cgtggcggcg
      721 ggcgcgaccg tggtcggcct cgagccttcg tgcctgctgt cacttcgcga cgagttcctc
      781 gtgatggggc tgggcgacag cgccgtgcag ctggcgccag gggcgctgat gatcgaggag
      841 ttcctcgcgc gggaacacaa ggagggacgg ctgcgactgc cgcttcaggc gctgccgcag
      901 aagcaggcgt tggtgcacgg ccattgccac cagaaagcat tcgatgtgct caacccggtc
      961 gtcaccatgc tgcggtgggt gccggagctg caggtcgagg tcatcgagtc gagctgctgc
     1021 ggcatggccg gcagcttcgg ttacgaggcc gcgcactacg gcgtgtcgat gaagatggcc
     1081 gaactgtcgt tgctgccggc ggtccgcaat gccgaagccg gaacgctgat catcgccgac
     1141 ggcaccagct gccggcacca gatcgccgac ggcacccatg ctgcgcgcga ggccgtgcat
     1201 gcgatccgcg tactggcgag tgctcttgct cctggcaatt agcgcgggct gcgttctggc
     1261 gtgcgcagca gggctcgcgc ccgctcgatg accgggcggt caacgagctt gccgtcgagt
     1321 cgcaaggcgt tgtcgccggc ttccgtcgcc gcggcgacaa cgcgacgcgc ccattccagg
     1381 tcggccggcg atggcgagaa gccctcgttg atcgcggcga cctggcgtgg gtggacggcg
     1441 agcttgcctc caaagcccag gcggcgcgca taggccacgt cgcgtaacag cgcttccgtg
     1501 tcgtcgagtg tcatcgtga
//
LOCUS       DATIRT010000318         1990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_48501, whole genome shotgun sequence.
ACCESSION   DATIRT010000318 DATIRT010000000
VERSION     DATIRT010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48501"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..152
                     /locus_tag="VLN25_07305"
     CDS             <1..152
                     /locus_tag="VLN25_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006494392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HSQ10406.1"
                     /translation="DGEIMGLRHKTLPVEGVQFHPESIATEHGHTMLRNFLAPSGGSK
                     GVPCR"
     gene            143..1174
                     /gene="trpD"
                     /locus_tag="VLN25_07310"
     CDS             143..1174
                     /gene="trpD"
                     /locus_tag="VLN25_07310"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HSQ10407.1"
                     /translation="MSITPSEALVRCIEHREIFQDEMLSLWRRLMSGELTPVQIAALT
                     MGLRVKKETIGEIAAAAQIMREFATKVEPADRSNLVDLCGTGGDAAHTFNISTASMFV
                     AAAAGARVAKHGGRSVSSGSGSADVLESLGANIVLTPAAVATCIAETGIGFMFAPSHH
                     AAMKHAAPVRKELGVRTIFNILGPLTNPAGAPNQVMGVFHPDLVGIQVRVLQQLGSNH
                     VLVVHGKDGMDEVSLGAATLVGELKDGAVREYEIHPEDFGLSMVSNRGLKVGSPDESR
                     SMVLEALDDVPGTPREIVIFNAGVALYAANVADSIGEGIAQARSAIASGAARRKLDEF
                     VATTRRLAA"
     gene            1222..1698
                     /locus_tag="VLN25_07315"
     CDS             1222..1698
                     /locus_tag="VLN25_07315"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSQ10408.1"
                     /translation="MHNIVLRAATRRDVAGVAALGASAARTEHALIPDVASRRHDVRE
                     ARRYWRRAIDSPRTRLFVAVRGAELLGAIGVDLIVSRHRLAHVRRRVYLHSLFIVPSA
                     RRLGLARRLTRLALDWARGRGATQARLEMAAPNRSARRLYEAVGFRAREMMFARPL"
     gene            1708..>1990
                     /locus_tag="VLN25_07320"
     CDS             1708..>1990
                     /locus_tag="VLN25_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase TrpC"
                     /protein_id="HSQ10409.1"
                     /translation="MDDILQRILATKHDEVRLARARVPIARLRERARDAAPALDFTAA
                     LVAAIRTGRSAVIAEVKKASPSKGVLRADFDPSAIGASYARHGAACLSVL"
BASE COUNT          315 a          646 c          699 g          330 t
ORIGIN      
        1 tcgacggcga gatcatgggt ctgcgtcaca agacgctgcc ggtcgagggc gtgcagttcc
       61 atccggagtc gatcgccacc gaacacgggc acacgatgct gcgcaacttc ctggcgccgt
      121 ccggtggatc gaaaggagtg ccatgtcgat aaccccgagc gaagcgctgg tccggtgcat
      181 cgaacaccgc gagatcttcc aggacgaaat gctgtcgctg tggcggcggc tgatgagtgg
      241 cgaactcacg ccggtgcaga tcgcggcgtt gacgatgggc ctgcgcgtca agaaggagac
      301 catcggggag atcgccgcgg cggcgcagat catgcgcgag ttcgcgacca aggtggagcc
      361 ggccgaccgt tccaacctcg tcgacctgtg cggcacgggc ggggatgcgg cgcatacgtt
      421 caacatctcg acggcctcga tgttcgtcgc cgcggcggcc ggcgcgcgcg tggccaagca
      481 cggcgggcgc agtgtgtcgt ccgggtcggg gagcgccgac gtcctggaat cgctcggggc
      541 aaatatcgtg ctgacgcccg ccgcggtggc aacctgcatc gcggagaccg gcatcggatt
      601 catgttcgcg cccagccatc atgccgcgat gaagcacgcc gcgccggtac gcaaggaact
      661 cggtgtacgc acgatcttca acattctcgg gccactgacg aatcccgccg gcgcaccgaa
      721 ccaggtgatg ggcgtgttcc acccggacct ggtcggcatc caggtgcgcg tgctgcagca
      781 attgggttcg aaccacgtgc tggtcgtgca tggcaaggac ggcatggatg aggtctcgct
      841 tggcgcggcg acgctggtcg gagaattgaa ggacggcgca gtgcgcgagt acgagattca
      901 cccggaggat ttcggcctgt cgatggtctc taatcggggt cttaaggtgg gcagcccgga
      961 cgaatcgcgc tcgatggtgc tcgaagcgct cgacgatgtc ccgggaacgc cgcgcgagat
     1021 cgtgatcttc aacgccggcg tggcgctgta cgccgccaac gttgccgact cgatcggcga
     1081 aggcattgca caggcgcgct ctgccatcgc cagcggcgcc gcgcgccgca agctcgacga
     1141 gtttgtcgcg acgacccgaa ggctggctgc gtgatcgttg cgcgcacgcg tgggcccttg
     1201 tgccgggtcg tacgcgctcg cgtgcacaac atcgtcctgc gggcagcaac gcggcgcgac
     1261 gtcgccggcg tcgctgccct gggcgcctcg gctgcaagga ccgagcacgc cctgataccg
     1321 gacgtcgctt cgcggcggca cgacgtgcgc gaggcgcgcc gctactggcg gcgggccatc
     1381 gacagcccgc gtacacgact cttcgtcgca gtccgggggg ccgagttgct gggtgcaatc
     1441 ggcgtcgacc tgatcgtctc gcggcatcga ttggcgcacg tgcgtcgtcg cgtgtatctg
     1501 cactcgcttt tcatcgtgcc atcggcgcgc cggctcggcc tggcgcgtcg gctgacccgc
     1561 ctggcgctcg actgggcccg cggccggggt gcgacgcagg cccggctgga gatggcggca
     1621 cccaaccggt ctgcgcgtcg tctgtacgag gcggtcggtt tccgcgcgcg ggaaatgatg
     1681 ttcgcgcgac ctttgtagca ttggccgatg gacgacatcc tgcaacgcat cctggcaacc
     1741 aagcacgacg aagtgcggct tgcgcgcgca cgcgtgccaa tcgcacggct gcgtgaacgt
     1801 gcacgtgacg ctgcgcctgc gctcgacttc acggcagcgc ttgtggccgc catccgcacc
     1861 ggacgcagcg ccgtaatcgc cgaagtgaag aaggcctcgc cgtcgaaagg cgtgctgcgg
     1921 gccgacttcg atccttcggc catcggcgcg agctatgctc ggcatggcgc cgcctgccta
     1981 agcgttctca
//
LOCUS       DATIRT010000319         2977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_344183, whole genome shotgun sequence.
ACCESSION   DATIRT010000319 DATIRT010000000
VERSION     DATIRT010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344183"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /gene="hypB"
                     /locus_tag="VLN25_07325"
     CDS             complement(<1..929)
                     /gene="hypB"
                     /locus_tag="VLN25_07325"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HSQ10410.1"
                     /translation="MCTTCGCGSGETRIEGTADNPAHELEHVHPDGTVHRHAHGHDGE
                     HAHEHVMPDGSVVRHMHAHDGATDLHYGHGPAHTHAPGMSQAKMVQIEQDILSKNDRH
                     ARFNREFLAERGIFAVNLVSSPGSGKTTLLTDSIQRLQPATAVTVIEGDQQTTHDADR
                     IRATGARALQINTGKGCHLDASMVERALERLQPDEGSVLFIENVGNLVCPAAFDLGEA
                     HKVVVLSVTEGEDKPLKYPDMFRAASLMLLNKLDLLPYVTFDVARCIEYARRVNPSIE
                     VMQVSATKGDGMDLWINWIRLHAVTARHAREQHV"
     gene            complement(952..1362)
                     /gene="hypA"
                     /locus_tag="VLN25_07330"
     CDS             complement(952..1362)
                     /gene="hypA"
                     /locus_tag="VLN25_07330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HSQ10411.1"
                     /translation="MSLAEGILQIVEDTARANAATQVRSVLLELGALSHVEEQALRFC
                     FDAVTRGTVADGARLDVHATAGRAWCMPCGESVALNRLGDDCPLCGGYQLQVTHGDEM
                     KVKEIEVDVETPTVTGFAAPQEGRQSAWERPGAD"
     gene            complement(1364..2449)
                     /locus_tag="VLN25_07335"
     CDS             complement(1364..2449)
                     /locus_tag="VLN25_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HSQ10412.1"
                     /translation="MNPEGELIVRLGLKANRVHAVNIASSRVALPERMTRGRSADKVA
                     RNLPLLFSICAKAQSAAAAGALDAARGCTPDAAIQQKRADDVRREAIVELLTRLLIDW
                     PRVMGAVPDVSSVARARQALPAQALDTWRSIARERIYGVAPDDWIADASLAALDRWAA
                     AAETLPARLFSQLHRESSDLGRSDICTMPAATLEALLPMLPRLADDPTFCQSPDWLGS
                     PVETGALARYAEHVLVATYVQRHGNTVAARTLARLVELAALLSVGNNPGVPSVRQHAV
                     APGTGIGLAETARGLLLHHAEVSEGRVNRYRIVAPTEWNFHPDGALSRGLRERHVDGP
                     ETARRETQILVQALDPCVACRVEVVDA"
     gene            complement(2433..>2977)
                     /gene="hybE"
                     /locus_tag="VLN25_07340"
     CDS             complement(2433..>2977)
                     /gene="hybE"
                     /locus_tag="VLN25_07340"
                     /inference="COORDINATES: protein motif:HMM:NF023365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="[NiFe]-hydrogenase assembly chaperone HybE"
                     /protein_id="HSQ10413.1"
                     /translation="PDPSPRLEQAFRATATRMSGLGFVNPALRVEAVAFAPWDGHWLG
                     VMVTPWSINLLLLPHDSSLWRTVRAGDKQRYAFPAGDYDFIAAMDPVVGEYQMCSLFS
                     PALEFEDHAAARLVATLAREALFDVSNAEQPEVNAATDDDGPPGPLERIQQQAGAPMS
                     KRDFLRGRFLRADESNEPGR"
BASE COUNT          463 a          995 c         1001 g          518 t
ORIGIN      
        1 acatgctgct cgcgtgcatg ccgcgcggtg accgcatgca gcctgatcca gttgatccac
       61 aggtccatgc catcgccctt cgtggcggaa acctgcatca cttcgatcga cgggttgacg
      121 cggcgcgcgt actcgatgca gcgcgcgacg tcgaaagtga cgtacggcag caggtcgagc
      181 ttgttcagca gcatcagcga cgccgcgcgg aacatgtccg ggtacttcag cggcttgtct
      241 tcgccttcgg tgaccgataa caccacgacc ttgtgcgctt cgccgagatc gaaggcggca
      301 gggcagacga gattgccgac gttctcgatg aacagcaccg agccctcatc gggttgcagc
      361 cgttcgagcg cacgctcgac catgctggcg tccaggtgac agcccttgcc ggtattgatc
      421 tgcaaggccc gtgcgccggt ggcgcggatg cgatcggcat cgtgcgtggt ctgctggtcg
      481 ccctcgatca cggtcactgc cgtggcgggc tgcaggcgct ggatcgaatc ggtcaggagc
      541 gttgtcttgc cggagccggg actcgagacc aggttgaccg cgaagatgcc gcgctccgcc
      601 aggaattccc gattgaagcg cgcgtgacgg tcgttcttgg aaaggatgtc ctgctcgatc
      661 tgcaccattt tcgcctggct catgccgggt gcgtgtgtgt gcgccggccc gtgaccgtag
      721 tgcaggtctg tcgcgccgtc gtgggcatgc atgtgtcgca cgacgctgcc gtcaggcatc
      781 acgtgctcgt gcgcgtgttc gccgtcatgg ccatgggcat gacgatggac ggttccgtcc
      841 gggtgcacgt gctcgagctc atgcgccggg ttgtccgccg tgccttcaat cctggtttcg
      901 ccgctgccgc agccacacgt cgtgcacatg atgtttctcc tcgatgccgc ctcagtcggc
      961 gccgggccgt tcccaagccg actggcggcc ctcttggggg gcagcgaacc cagtgaccgt
     1021 gggggtctca acgtcgactt cgatctcctt cactttcatc tcgtcaccgt gggtcacctg
     1081 cagttgatag ccgccgcaca acggacagtc gtcgcccagg cgattcaatg ccacgctctc
     1141 gccgcagggc atgcaccacg cccggccggc cgtcgcgtgc acatcgaggc gcgctccgtc
     1201 cgcaacagtg ccgcgcgtca ctgcgtcaaa gcagaagcgc agcgcctgct cttccacgtg
     1261 cgacaaagcg ccgagctcca gcaggactga ccgtacctgc gtagccgcgt tggcgcgggc
     1321 ggtgtcctcg acgatctgca ggatgccttc ggcgagcgac atttcatgca tcgacgacct
     1381 cgacacggca ggccacacag gggtcgaggg cctgcaccag gatttgggtc tcgcgacgag
     1441 ccgtttcggg tccgtcgacg tgacgctcac gaagcccgcg cgacagcgcg ccgtccggat
     1501 ggaagttcca ctcggtgggc gcgacgatcc ggtagcggtt gactcgtccc tcgctgactt
     1561 cggcatggtg cagcagcagc ccgcgggccg tctcggccag tccgatgccc gttcccggcg
     1621 cgacagcgtg ctggcgaacg ctgggcactc ctgggttgtt gccgacggac agcaatgctg
     1681 ccagctcgac tagccgcgcc agcgtgcgcg ccgccaccgt gttgccgtgc cgttggacat
     1741 aggtggccac cagcacgtgc tcggcatacc gcgcaagggc gccggtctcg accgggcttc
     1801 cgagccagtc gggcgattgg cagaacgtcg gatcgtcggc gagccgcggc aacatgggga
     1861 ggagggcttc gagcgttgcg gcgggcatcg tgcagatatc gctgcggccc agatccgatg
     1921 actcccggtg cagctggctg aaaaggcgcg ccggcagagt ctctgccgcc gcggcccagc
     1981 gatcgagcgc tgccagcgag gcgtcggcga tccagtcgtc gggggcgact ccgtagatgc
     2041 gctcgcgtgc gatgctgcgc cacgtatcga gcgcctgcgc cggcaatgcc tggcgtgccc
     2101 gggcgacgct gctcacgtcc gggacggcgc ccatgacgcg cggccagtcg atcagcagac
     2161 gggtcaggag ctcgacgatg gcttcgcggc gcacgtcatc cgcgcgcttc tgctggatcg
     2221 cggcgtcggg cgtgcatccg cgcgccgcat cgagcgcgcc cgcggcggct gccgactgcg
     2281 ctttggcgca gatcgagaag agcagcggca gattgcgtgc gaccttgtcc gccgatcgtc
     2341 cgcgcgtcat gcgctccggc agcgccaccc ggctcgaagc aatgttcacg gcatgcacgc
     2401 gattcgcttt caaacccagc cgcacgatca gttcaccttc cgggttcatt ggactcgtcc
     2461 gcgcgcaaga agcggccgcg aaggaagtcg cgcttggaca tgggcgcacc cgcttgttgc
     2521 tgtatccgtt ccaggggtcc aggcgggccg tcgtcatcgg tcgccgcgtt gacctcgggt
     2581 tgctccgcgt tgctgacatc gaacagggct tctcgcgcta gcgtcgccac caaccgagcg
     2641 gccgcatggt cctcgaactc cagggcgggt gagaagagcg aacacatctg gtactcgccc
     2701 acgaccgggt ccatcgccgc aatgaagtcg tagtccccgg cggggaacgc atagcgctgc
     2761 ttgtcgcccg ctcggaccgt gcgccacagc gaggagtcgt gcggaagcag caacagattg
     2821 atcgaccaag gggtcaccat gacgccgagc cagtggccat cccagggtgc gaacgcgacc
     2881 gcctcgacgc gcagcgccgg gttcacgaag cccagtccgc tcatgcgcgt ggccgtggcg
     2941 cgaaacgcct gctcaagccg tggcgagggg tcgggca
//
LOCUS       DATIRT010000320         1866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_344301, whole genome shotgun sequence.
ACCESSION   DATIRT010000320 DATIRT010000000
VERSION     DATIRT010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344301"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1220)
                     /gene="urtA"
                     /locus_tag="VLN25_07345"
     CDS             complement(<1..1220)
                     /gene="urtA"
                     /locus_tag="VLN25_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008333704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HSQ10414.1"
                     /translation="MDRRQFIQAGGVAALSCSSISTAFGQDKTPIKVGVLHSLSGTMA
                     ISETALKETALMTFAEINAKGGVMGRPIEPVVVDPASNWPLFAEKARQLITQDKVVAT
                     FGCWTSVSRKSVLPVFKELNSILYYPVQYEGEELEKNVFYTGAAPNQQAIPATEYLMS
                     KEGGAAKRFFLLGTDYVYPRTTNKILRAFLKSKGVAEKDIEEVYTPFGHSDYQTIVAN
                     VKKFAAGGKTCVISTINGDSNVPFYKELGNAGLKAKDVPVVAFSFGEEELRGVDTKPL
                     VGHLAAWNYFMSLKNPQNDAFIKMYREWAVKQKLPNADKVVTNDPMEATYVGIHMWKQ
                     AVEKAKSVEADKVIAAMSGQSFAAPCGFTIKMDETNHHLWKPVMIGEIRPDGQFNVVW
                     KTKGPVRAQPWSPF"
     gene            1410..>1866
                     /locus_tag="VLN25_07350"
     CDS             1410..>1866
                     /locus_tag="VLN25_07350"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HSQ10415.1"
                     /translation="MSRKTPPASRVPQSRFGRLAQLGLAAGELAVGGAMQGLRRLGRG
                     DASAPRFSVATVRRLVDRLAHLRGAAMKIGQLISLEAEGLLPPELVIALEALRSQAAP
                     MPLEQLRRALGRAYGSGWERRFTQFDEVPIASASIGQVHRARTADGRDLA"
BASE COUNT          298 a          592 c          592 g          384 t
ORIGIN      
        1 atgaaggggc tccacggttg tgcacgcacc gggcccttcg tcttccatac gacgttgaac
       61 tggccgtccg gacggatctc accgatcatc accggcttcc agaggtggtg attggtttca
      121 tccatcttga tcgtgaatcc gcacggtgcg gcgaaggatt ggccgctcat ggcagcgatc
      181 accttgtcgg cctcgactga cttggccttc tcgaccgcct gcttccacat gtggatgccg
      241 acgtaggtcg cttccatcgg gtcgttggtc acgaccttgt cggcgttggg cagcttctgc
      301 ttgaccgccc attccctgta catcttgatg aacgcatcgt tctgcggatt cttcagcgac
      361 atgaagtagt tccatgccgc gaggtgaccg accagcggct tggtgtctac gccgcgcagt
      421 tcctcttcgc cgaacgagaa cgccacgacc gggacatcct tcgccttcag cccggcattg
      481 ccgagttcct tgtagaacgg cacgttcgag tcgccgttga tggtcgagat gacgcaggtc
      541 ttgccgccgg ccgcgaactt tttgacattg gcgacgattg tctggtagtc actgtgaccg
      601 aagggcgtat agacctcctc gatatccttc tccgccacgc ccttgctctt gaggaaagcg
      661 cgcaggatct tgttggtcgt acgcgggtag acgtagtccg tgccgagcag gaagaagcgc
      721 ttggcggcgc cgccttcctt gctcatcaga tattcggtcg cagggatcgc ctgctggttc
      781 ggcgcggcgc ccgtgtagaa gacgttcttt tcgagctctt cgccttcata ctgcaccggg
      841 taatacagaa tcgagttgag ttccttgaac accggcaaca ccgacttgcg tgacaccgac
      901 gtccagcaac cgaacgtggc aaccaccttg tcctgcgtta tgagttggcg cgccttctct
      961 gcgaacagcg gccagttcga cgccggatcg acgacgacgg gctcgatcgg acgccccatc
     1021 acgccgccct tggcattgat ctccgcgaaa gtcatcagcg ccgtttcctt caacgccgtc
     1081 tcggagattg ccatcgtgcc tgacagcgaa tgcaggacgc cgaccttgat cggtgtcttg
     1141 tcctggccaa acgcggtcga aatcgaacta cagctgaggg cggcaacacc cccggcctgg
     1201 atgaattgtc tgcggtccat tcctctctcc agtagcagca gcgcacattc gccgcattgc
     1261 agtgcgatac aagttccgtg ccatcccgtt tcagtgcgtt ttgcctcggc caggcggggt
     1321 tgccgcacca caagcgggtg gaatgcactg gcgcggctgt gcgcgcgccc ttgcgcggtg
     1381 cggcgatccg tgcgcggcga aggggcgcga tgagccgcaa gactccaccg gcctcgcgcg
     1441 tcccgcagag tcgattcggg cggctggcgc agctcggatt ggcggcgggc gaactggccg
     1501 tcggcggggc gatgcaggga ttgcgtcgac tcgggcgggg agatgcctcg gccccgaggt
     1561 tctccgtcgc caccgtgcgc cgcctggtcg accggctggc ccatctgcgc ggcgcggcga
     1621 tgaagatcgg tcagctgatt tcgctggaag ccgaaggcct gttgccgccg gaactcgtga
     1681 ttgcgctgga agcgctgcgc tcgcaggccg caccgatgcc gctcgagcag ctgcggcgcg
     1741 cgctgggccg ggcctatggc tcaggctggg agcggcgctt cacgcagttc gacgaggtgc
     1801 cgatcgcgtc ggcgtcgatt ggccaggtgc atcgcgcacg cacggcggac ggtcgcgacc
     1861 tcgcgc
//
LOCUS       DATIRT010000321         3613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_245926, whole genome shotgun sequence.
ACCESSION   DATIRT010000321 DATIRT010000000
VERSION     DATIRT010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245926"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..869
                     /locus_tag="VLN25_07355"
     CDS             81..869
                     /locus_tag="VLN25_07355"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HSQ10416.1"
                     /translation="MTLLIDIGNTALKWAFTDGRSEPAEVHVELHLGDADLAVRLAAA
                     WASMPSGLPAVGCSVAASPVRAAVERAAKARGATVHWLGAEPRHDGEVVLVNGYRNPA
                     QLGADRWHGMLGACLRCPGQSFVLVAAGTATTVDCVQRGPDVAGFIGGCIAPGARMMF
                     EALARRTAGLPQARGAATDFPDNTDDAITTGVVDAQAGLAGQIVQRFARRLGHAPAIL
                     VSGGDAEAVADRLRTSGLGAVIEHNLVLAGLALRARVSNSTMDR"
     gene            866..1576
                     /locus_tag="VLN25_07360"
     CDS             866..1576
                     /locus_tag="VLN25_07360"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HSQ10417.1"
                     /translation="MKLAFLFLLLVNVALFAWQQGAFGRFAESGREPERIARQIEPDR
                     IRVLTEKDVQILRERASQARASAAVASTPSTPAVPDLSVAQACVEFGDFPAADVTRVE
                     TALVKLGLGSRQTTRSVDVPGWYLVYVPPFQTRAEADRAAADFTKRGVKDLLVLTEGS
                     LRLGISLGSFKDPELAKAHVAALDKLGVKNARIGDKPTSVSATRYQLRELDAGTAQQL
                     AAIRKEFPAQSVRPCGNG"
     gene            complement(1712..2146)
                     /locus_tag="VLN25_07365"
     CDS             complement(1712..2146)
                     /locus_tag="VLN25_07365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HSQ10418.1"
                     /translation="MTALPCELCTGPGGRVLHNDGRLRVVVVDDPDYPGFMRVIWNAH
                     VREVTDLSPAERDHFMQAVFAVERAQRTVMRPYKMNVASLGNMTPHLHWHLIPRFEDD
                     AHFPKPIWADRQRRATAASLAARTGQLPALERATVAALTASA"
     gene            complement(2143..3111)
                     /locus_tag="VLN25_07370"
     CDS             complement(2143..3111)
                     /locus_tag="VLN25_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSQ10419.1"
                     /translation="MNRQITVRLAHATADDDEIARIITAVHPDWPRTGAEMAAAARRR
                     GERFHLKLVAENAPGTLAGYGFLEVPDVAAAEGRLRVRVTLDPASVGRGIATALYDAL
                     ESEARQRGTIELCTEVLASNPRAERFALARGFAVYNRRIESRLSLGTIVPEHIARGID
                     SHTDRLFSTGIRIASYRQLALAVATAPRHLYDLIVELWRDVPFGISGADPTFESFVSE
                     ELDDPAFSPAGTFVALDGVTWIGLCAQTSAEGRLMTSMTGVVRAWRRHGLARWLKLHS
                     IRYALEVEAREIRTFNDAANEGMLTLNRALGFVPVETDLRMKKELR"
     gene            complement(3146..>3613)
                     /locus_tag="VLN25_07375"
     CDS             complement(3146..>3613)
                     /locus_tag="VLN25_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3400 domain-containing protein"
                     /protein_id="HSQ10420.1"
                     /translation="EYLLEKGVRIEGVGGVRYMYHDPCHSPMKQQDPLITVNALVATA
                     DGTSIGKNDRCCGESGTFAISRPDIATQVRFRKEREITAAAARLRADGFDGAVKVLTS
                     CPSCLQGLKRYNDDADMDADYIVVELARHVLGADWMPEYVARANTGGIERVLV"
BASE COUNT          558 a         1187 c         1256 g          612 t
ORIGIN      
        1 gggccgcctg atgctggcca ctgcgggcgg cagccgcagt atcaacgtcg gcgatgtttc
       61 cctgcgcgct gcgccgtccg atgacgctgc tgattgacat cggcaacacc gcgctgaaat
      121 gggcgttcac ggatggccgc agcgagcccg ccgaggtgca cgtcgaactg cacctcggtg
      181 acgccgacct tgcggtgcga ctggccgcgg cgtgggcgtc gatgccttca ggcctgccgg
      241 cggtgggatg cagcgtggcc gcgtcgccgg tccgcgcggc ggtggagcgt gcagccaagg
      301 cacgaggcgc gacggtgcac tggctcggtg ccgagccacg tcacgacggt gaggtcgtgc
      361 ttgtcaacgg ctatcgcaat cccgcccagc tcggggccga tcgctggcac ggcatgctgg
      421 gagcctgcct gcgttgcccc gggcaatcgt tcgtgctggt cgcggcgggg acggccacca
      481 cggtcgactg cgtgcagcgt ggccccgacg tggcgggctt cattggtggc tgcattgcgc
      541 cgggggcacg gatgatgttc gaggcactgg cccgccgtac ggcgggattg ccgcaggccc
      601 gcggcgcggc gacggatttc ccggacaaca ccgacgatgc catcacgacg ggcgtcgtgg
      661 acgcgcaggc cggattggcc ggtcagatcg tgcagcgctt tgcgcgccga ctgggacatg
      721 cgccggccat tctggtcagc ggtggcgacg ccgaagccgt tgccgatcgt ctgcggacca
      781 gcggactcgg tgccgtgatc gaacataatc tcgtcctggc cggcctcgcg cttcgtgcgc
      841 gggtttccaa ctcgacgatg gaccgatgaa gcttgcgttt ctcttcctgc tgttggtgaa
      901 tgtggcgctc ttcgcgtggc agcagggagc gttcggccgc tttgcggaaa gcgggcgcga
      961 gccggagcgc atcgcccggc agatcgaacc cgatcgcatc cgcgttctta ctgaaaagga
     1021 tgtgcagatc ctgcgcgagc gcgccagcca ggcgcgcgca tcggcggccg tcgcgtcgac
     1081 gccctccact cccgcggtgc ccgatctgtc ggtggcccag gcctgtgtcg agttcggcga
     1141 tttccccgcg gcggacgtga cgcgcgtcga gaccgcgctg gtcaagctcg gcctggggtc
     1201 gcgccagacc acgcgcagcg tcgacgtgcc gggctggtac ctcgtctacg tgccgccatt
     1261 ccagacgcgg gcggaagccg accgcgccgc ggctgacttc acgaagcgcg gcgtcaagga
     1321 tctgctggtc ctgacggaag gttccttgcg gctcgggatc agcctgggat cgttcaagga
     1381 tcctgagctt gccaaggcgc atgtcgcagc gctggacaaa ctgggcgtga agaacgcgcg
     1441 cattggggac aagccgacgt cggtcagcgc gacgcgctac cagttgcgcg aactcgatgc
     1501 cggtacggcc cagcagctgg ccgcgatccg caaggaattc ccggcgcagt cagtgcgtcc
     1561 gtgcggcaac ggctgacctg gggtggtcgc gacgcctgcg cgcggcccgc ggcgcctgga
     1621 caatcgcacc cgctgaaatt ccacggcaac gtgtcgccgc aatccctgtc gcccaagccc
     1681 gttacccccc ccggggcccg cgccgcctgg ctcaggcgga cgccgtcagt gcggctaccg
     1741 tggcccgctc gagtgccggc agttgacccg ttcgtgccgc aagcgaggcg gcggtcgccc
     1801 tgcgttgacg gtcggcccag atcggtttcg ggaagtgcgc gtcgtcctca aagcgcggaa
     1861 tgaggtgcca gtgaaggtgg ggcgtcatgt tgccgagact cgcgacgttc atcttgtagg
     1921 ggcgcatgac ggtccgctgc gcccgctcga cggcgaacac ggcctgcatg aagtggtcgc
     1981 gctccgccgg tgacaggtcg gtcacttcgc gcacgtgcgc attccagatg acgcgcatga
     2041 atccggggta gtccggatcg tcgacgacca cgacgcgcag ccgcccgtcg ttgtggagca
     2101 cgcgccctcc gggtcctgtg cacagttcgc atggcagcgc agtcatcgca gctccttttt
     2161 catccgcagg tcggtctcga ccggcacgaa gcccagtgcg cgattcagcg tcagcatgcc
     2221 ctcgttcgcc gcatcgttga aagtgcggat ctcccgggct tcgacctcga gcgcatagcg
     2281 gatcgaatgc agcttcagcc agcgcgccag tccatgccgg cgccacgcgc ggacgacgcc
     2341 ggtcatcgag gtcatcaggc ggccctcggc tgaggtctgt gcgcacagac cgatccaggt
     2401 cacgccgtcg agagcgacga aggtcccggc ggggctgaaa gccgggtcgt cgagctcctc
     2461 gctgacaaaa ctttcgaacg tcggatccgc tccgctgatg ccgaacggca cgtcgcgcca
     2521 cagctccacg atcaggtcat acaggtggcg cggtgcagtt gcgacggcga gcgcgagctg
     2581 ccggtaggag gcgatacgta tgccggtact gaagagacgg tctgtgtgcg agtcgatgcc
     2641 gcgggcgatg tgctcgggca cgatcgtccc gagcgatagc cggctttcga tgcgacggtt
     2701 gtagacggcg aagccgcgag cgagggcgaa tcgctcggcg cgcggattcg aggcgagtac
     2761 ttcggtacac agttcgatcg tgcctcgctg tcgcgcttcc gactcgaggg cgtcgtacag
     2821 cgcggtggcg ataccgcgcc cgacgctggc cgggtccaga gtaactcgca cgcgtagcct
     2881 cccctcggca gcggccacgt ccgggacctc gaggaatccg tacccggcta gtgtccccgg
     2941 agcgttttca gcgacgagct tcaggtgaaa gcgttcaccg cgacgacgtg cagccgccgc
     3001 catctcggcg ccagtgcggg gccagtccgg atgcactgcg gtgatgatgc gtgcaatctc
     3061 gtcatcgtcc gccgtggcat gagccaagcg gaccgtgatc tgccgattca tgcggcgcga
     3121 tctaggcgcg accacggctg cgtggctaga ctagtacgcg ctcaatgccg ccggtgttgg
     3181 cgcgggccac gtactccggc atccagtccg cgccgagcac atggcgagcg agttccacga
     3241 cgatgtagtc ggcgtccatg tcggcatcgt cgttgtagcg tttcagcccc tgcaggcacg
     3301 acgggcagga tgtcaggacc ttgaccgcac cgtcgaaacc gtcggcacgg agtcgcgcgg
     3361 cggcagcggt gatctcgcgt tccttgcgga agcgcacctg ggtcgcgata tcgggacgcg
     3421 aaatcgcaaa cgtgccggac tccccgcagc agcgatcgtt cttcccgatc gacgtgccgt
     3481 cggccgtggc caccaaagca ttgaccgtta tgagtggatc ctgctgcttc atcgggctgt
     3541 ggcaggggtc gtgatacatg tagcgcacgc caccgacacc ctcgatgcgg acaccctttt
     3601 caagcaggta ctc
//
LOCUS       DATIRT010000322         6412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1623236, whole genome shotgun sequence.
ACCESSION   DATIRT010000322 DATIRT010000000
VERSION     DATIRT010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6412
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1623236"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1300)
                     /locus_tag="VLN25_07380"
     CDS             complement(<1..1300)
                     /locus_tag="VLN25_07380"
                     /inference="COORDINATES: protein motif:HMM:NF036554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HSQ10421.1"
                     /translation="MRLGLLALIVSLLGLPVGVQAQSAAPEDDGVRLVTVSGVSGQVA
                     RALEDSLARFRLAPEADEADEERELRRAERAATEVLATEGYFSPRVRFEPNPAGDPRY
                     RLVVEPGQLTRVTAVEISFRSALAEAAFTERAADLKKGWTLPVGAPFRSADWEIAKNR
                     LLLATTARDFAAATLTHSSADVDVESGAAVVRVEVDSGPAYRVGSLRVEGLERHDAAL
                     VERFNPFQPGQPYDRALVLQFQQALQETPFFTSVVVNLDLAAAVADSAPLKVQVREAL
                     TRRFSAGIGYATNTGAHLEAVYRQNITFGQPYLLQTGFRIDQTGEFAYADLIFPPQPG
                     GARDSVGVLVENSDIENLQINRWGIGAARSKLTGPRDGRNVETQLALNFEHERRETPL
                     DPPITIDVLSTTYTWTRRDVDDITHPRKGNLIQLQGSVGIG"
     gene            1477..4062
                     /gene="clpB"
                     /locus_tag="VLN25_07385"
     CDS             1477..4062
                     /gene="clpB"
                     /locus_tag="VLN25_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HSQ10422.1"
                     /translation="MRIDKLTTKFQEALGDAQSIAAGHDNSYIEPVHVLLAMLRQTDA
                     GTRSLLQRAGANIGALTSALDAAIKRLPQVKGGDGQIQIGRDLTSVLNAADKEAQKHG
                     DSYIASEMFLLAVADDKGEAGRLAREHGVSRKALVAAIDAVRGGAPVESAEAEGQREA
                     LKKYTIDLTERAQQGKLDPVIGRDDEIRRTIQILQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNDEVPETLKGKRVLVLDMAGLLAGAKYRGEFEERLKAVLKEVSAEAGRIILF
                     IDEMHTMVGAGKAEGAIDAGNMLKPALARGELHCIGATTLNEYRKYIEKDAALERRFQ
                     KVLVDEPSVEATIAILRGLQERYELHHGVEITDPAIVAAAELSHRYITDRFLPDKAID
                     LIDEAAARIKMEIDSKPESMDKLDRRLIQLKIEREAVKKEHDEASKKRLALIEEEIKR
                     LGKEYADLDELLRAEKAAVQGAASIKTEIDKLKAQLPALQRSGKYEEAARIQHEQLPK
                     LEMQLKVAEKAEAPKGKPRLLRTQVGAEEIAEVVSRATGIPVSKMMQGEREKLLKMED
                     YLHRRVVGQDEAVRLVSDAIRRSRAGLADPNRPYGSFLFLGPTGVGKTELTKALAEFL
                     FDTEDALIRIDMSEYMEKHSVARMIGAPPGYIGYDEGGALTEAVRRKPYAVILFDEVE
                     KAHPDVFNVLLQVLDDGRMTDGQCRTVDFKNTVIVMTSNLGSHDIQRMAGQDPELIKD
                     TVMGEVRKHFRPEFVNRIDEIVVFQALDSRQIKAIAKIQLKRLEQRLAAQDMKLDVSD
                     AALAEVAKVGFDPLYGARPLKRAIQQEIENPVARLVLEGRFGPKDVIPVDVKNGKFVF
                     DRIVH"
     gene            complement(4101..5294)
                     /locus_tag="VLN25_07390"
     CDS             complement(4101..5294)
                     /locus_tag="VLN25_07390"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HSQ10423.1"
                     /translation="MALFATAGCGTLIRNPVPPELTTSAIVPGMPDVRAWAGHASQAL
                     QDDIVLSYKQESRAEFPVGADGRVQYSHLLLSGGGANGAFGSGLLNGWTKAGSRPVFK
                     IVTGVSTGALMAPYAFLGPKYDDALRDLYTTTRSQNIFVSGSLFGMISQLFFGEALTD
                     TGPLASMIAQHVDEQFVREVAAAHSQGRRLYIGTVELDAQRFVVWNMGLIAQADYPDK
                     VRLFRKVMLASASIPVVFPPVFFDVEAGGTLFDEMHVDGGVAAMVFFNAGLFSPLVAR
                     STVGAAPGYDRIFVIHNGQLAPPTPTPTPRTVRGISSRVLEAAGRSAVIGDLFRIYTI
                     AQREGAEYRWVTIPDDVSLRGDEFFDPVLMTSLYELGFKRGLAGGAWNMRLPGELHHL
                     RGRAE"
     gene            5679..>6412
                     /locus_tag="VLN25_07395"
     CDS             5679..>6412
                     /locus_tag="VLN25_07395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSQ10424.1"
                     /translation="MRKEFSSLLRRSVPSHAFRPAAIPVGLLIAVLALAGCRKAAEAP
                     KEEIRPVRVVTIDRIASGDSVNLTGTVQAETEVNLSFRIDGRMIERSIGVGDAVTAGK
                     VIARLDPSNEQTALQSARATLAGTRAQLVEQRNNFQRQKDLLAQNFISKAAFDLTAAN
                     LQSAESAAQSAQSQVDLAQNRLSYTQLVADAPGVVTAVGAEAGEVVPAGRMVIQVARK
                     GGRDAVFDVPAQVKDSAPANPEITVVL"
BASE COUNT         1243 a         1988 c         2143 g         1038 t
ORIGIN      
        1 cgccgatacc caccgacccc tgcagctgga tcaggtttcc ttttctcgga tgggtgatgt
       61 cgtcgacatc gcggcgggtc caggtatagg tggtcgacag cacgtcgatc gtgatgggag
      121 gatcgagcgg tgtctcgcgg cgttcgtgct cgaagttcag ggccagctgc gtctcgacat
      181 tgcggccgtc acgcggtccc gtcagcttcg agcgcgccgc gccaatgccc cagcggttga
      241 tctgcaggtt ttcgatgtcc gagttttcaa ccagcacgcc gacgctgtcg cgagcaccgc
      301 ccggctgcgg cgggaagatc aggtcggcat aggcgaactc accggtctgg tcgatgcgaa
      361 agccggtttg cagcaggtac ggctgaccga aggtgatgtt ctgtcggtac accgcttcaa
      421 gatgggcgcc tgtattggtc gcgtagccga tgcccgccga gaagcgccgc gtcagcgcct
      481 cgcggacctg caccttcaac ggcgcactgt cagcaacggc ggcagccagg tccagattga
      541 cgacgacgct cgtgaaaaag ggcgtttcct gcagggcctg ctggaactgg agtaccagcg
      601 cgcgatcgta gggctggccg ggctggaacg ggttgaagcg ttcgaccagc gcggcatcgt
      661 gccgttcgag accctcgacc ctcaacgatc cgacacggta cgcgggaccg ctgtccactt
      721 cgacgcggac gaccgccgcg ccggattcga cgtcgacgtc ggcgctcgaa tgagtgaggg
      781 tcgcggccgc gaagtcgcgt gccgtggtgg ccagcagcaa ccggttcttg gcgatttccc
      841 aatcggcgct tcgaaacggc gccccgaccg gaagggtcca gcctttcttc aggtcggccg
      901 cccgctcggt gaacgccgcc tcggccaacg cgctgcggaa actgatctcg accgcggtga
      961 cccgggtcag ttgtcccggt tcgaccacca gtcgatagcg cggatcgccg gcgggattcg
     1021 gctcgaagcg cacgcgcggg ctgaagtatc cctcggtggc caggacctcc gtggcggctc
     1081 gctcggcgcg tcgcagctca cgctcttcgt cggcttcgtc ggcctcgggt gcgagccgga
     1141 agcgcgccag actgtcttcg agcgcgcggg cgacttgccc gctgacaccc gacacggtca
     1201 ccaggcgcac accgtcgtcc tcgggcgccg cagactgcgc ctgaacgcca acaggcaggc
     1261 cgagcaggga gacaatcagt gccagcagtc caaggcgcac agtgcagtcc tgaatcgacg
     1321 gcgacagccg caaaggacgg tggtcccaac ggaagcaggc ggccgccatc tgcgaccgat
     1381 gctagcaaag caacggaaac ggcacattgg cttgaaaacg cgcgcgatcg cctcacgttg
     1441 aatagggaaa acagtcttcg ggggatgggc acatctatgc gtatcgacaa actgactacc
     1501 aagttccagg aagcgctcgg cgatgcccaa tcaatcgccg cagggcatga caactcgtac
     1561 atcgaacccg tgcatgtgct gctggcgatg ctgcgccaga ccgatgccgg gacgcgctcg
     1621 ctactgcagc gcgccggtgc caacatcggc gcgctgacgt cggccctcga tgcggcaatc
     1681 aagcgcctgc cacaggtcaa ggggggcgat ggccagatcc agatcgggcg cgatctcacc
     1741 agcgtgctca atgcagccga caaggaagcg cagaagcacg gagacagcta catcgcgagc
     1801 gagatgttcc tgctggcggt agcggacgac aagggcgagg cgggccgtct tgctcgcgag
     1861 cacggtgtga gccgcaaggc ccttgtagcg gcaatcgacg ccgttcgggg cggtgccccg
     1921 gtcgaaagcg ccgaggccga aggccagcgc gaggcactca agaagtacac gatcgacctg
     1981 actgaacgcg cgcaacaggg caagctcgac cccgtgatcg gccgcgatga cgagatccgg
     2041 cgcacgatcc agatcctgca gcggcgcacg aagaacaacc cggtgctgat cggcgagccc
     2101 ggtgtcggca agaccgcgat cgtcgagggt ctcgcgcagc gcatcgtcaa cgatgaagtc
     2161 ccggagacgc tgaagggcaa gcgcgtgctg gtgctcgaca tggcgggcct gctggccggc
     2221 gccaagtacc gtggtgaatt cgaggagcgg ctgaaggccg tgctgaagga ggtttccgcc
     2281 gaggcgggcc gcatcattct cttcatcgat gagatgcaca cgatggtggg cgccggcaag
     2341 gccgagggcg cgatcgacgc gggcaacatg ctgaagcccg cgctcgcgcg gggcgagctg
     2401 cactgcatcg gcgccaccac gctcaacgag taccgcaagt acatcgagaa ggatgccgcg
     2461 ctcgagcgcc gcttccagaa ggtgctggtc gatgagccga gcgtcgaggc gaccatcgcg
     2521 attctgcgtg gactgcagga gcgctatgag cttcatcacg gtgtcgagat cacggatccg
     2581 gccatcgtcg cggccgcaga actctcgcat cgctacatca ccgaccgctt cctgcccgac
     2641 aaggcgatcg acctgatcga cgaggcggcg gcgcgcatca agatggagat cgactccaag
     2701 ccggagtcga tggacaagct cgaccgtcgc ctgattcagc tgaagatcga acgcgaagcg
     2761 gtgaaaaagg aacatgacga agcgtcgaag aagcgcttgg cgctcatcga agaggagatc
     2821 aagcgcctcg ggaaggagta cgcggatctc gacgaactgt tgcgagcgga gaaggcggca
     2881 gtgcagggcg ccgcctcgat caagaccgag atcgacaagc tcaaggcgca gttgccggca
     2941 ctgcagcgca gcggcaagta cgaggaagcc gcgcgtatcc agcatgagca gcttcccaaa
     3001 ctcgagatgc agttgaaggt tgccgagaag gccgaggcgc cgaagggcaa gccgcgcctt
     3061 ttgcgcacgc aggttggcgc cgaagagatc gccgaggtcg tttcacgcgc gaccggcatt
     3121 ccggtcagca agatgatgca gggtgagcgc gagaaactgc tgaagatgga ggactacttg
     3181 catcggcgcg tcgtcggaca ggatgaagcg gtgcgcctgg tctccgatgc gatccgtcgc
     3241 tcgcgggcgg gcctcgcgga cccgaaccgt ccgtatggtt cattcctttt cctggggccc
     3301 acgggcgtgg gcaagaccga actgaccaag gcgctcgctg aattcctgtt cgacaccgaa
     3361 gacgccctga tccgcatcga catgagcgag tacatggaga agcactccgt ggctcgcatg
     3421 atcggcgcgc cgccgggcta catcggctat gacgaaggtg gagcgctgac ggaagccgtg
     3481 cgccgcaagc cgtatgcggt gatcctgttc gacgaagtcg agaaggcgca tccggatgtc
     3541 ttcaacgtgc tgctgcaggt gctcgacgat ggccgcatga cggacgggca gtgccgcacg
     3601 gtcgacttca agaacacggt catcgtgatg acttcgaatc tcggctcgca cgacatccag
     3661 cgcatggcgg gacaggaccc cgagctgatc aaggacacgg tgatgggcga ggtgcgcaag
     3721 cacttccggc cggagttcgt gaaccggatc gatgagattg tcgtgttcca ggcgctcgat
     3781 tcacgtcaga tcaaggcgat cgcgaagatc cagctgaagc ggctcgaaca gcgactggcc
     3841 gcgcaggaca tgaagctcga tgtcagcgac gcggcgctcg ccgaagtggc gaaggtcggt
     3901 ttcgatcccc tttacggagc acgtccgctg aagcgtgcga tccagcagga gatcgaaaat
     3961 cctgtggccc ggctggtgct cgagggccgc ttcggaccca aggacgtgat tccggtcgac
     4021 gtgaagaacg gtaagttcgt gttcgatcgg atcgtgcact gagcttccgg cgcccgcgcc
     4081 ggtgcgtacg ggccgagcga ctactcggcc cggccacgca ggtggtggag ttcgcccggc
     4141 aggcgcatat tccaggcgcc gcccgcaagg ccgcgtttga agcctagttc gtagagtgac
     4201 gtcatcaaca cggggtcgaa gaactcatcg ccccgcaggc tcacgtcgtc cggaatcgtg
     4261 acccagcggt actcggcccc ttcccgctgc gcgatcgtat agatgcgaaa caggtcgccg
     4321 atcacggcgg aacggccggc cgcttcgagc acgcggctcg aaatcccgcg gaccgtgcgc
     4381 ggtgtcggtg tcggcgtagg gggggctagc tggccgttgt ggatcacgaa gatccggtcg
     4441 tagcctggcg cggcgcccac cgtactccgc gcgaccagag gactgaagag tccagcattg
     4501 aagaagacca tggctgccac gccgccatcg acgtgcatct cgtcaaagag cgtgccgccg
     4561 gcttccacgt cgaaaaacac cggtggaaag actactggga tcgatgccga ggccagcatt
     4621 actttgcgaa acagcctgac cttgtccgga tagtccgcct gcgcgatcag gcccatgttc
     4681 cacacgacga agcgctgggc gtcgagttcg accgtgccga tgtagagtcg acggccctgc
     4741 gagtgcgccg cagccacctc gcgcacgaat tgctcgtcga catgctgcgc gatcatcgat
     4801 gcaagcgggc cggtgtcggt cagggcttcg ccgaagaaca gttgcgagat catgccgaac
     4861 agcgagccgc tgacaaagat gttctgcgac cgcgtcgttg tgtagagatc gcgcaacgca
     4921 tcgtcgtact tgggccctag gaacgcgtaa ggcgccatca gcgccccggt ggaaacgccg
     4981 gtaacgatct tgaacacggg gcggctaccg gccttcgtcc agccgttcag caggccggaa
     5041 ccgaaggctc cgttggcgcc gccaccggaa agcagcagat gcgagtactg gacgcgccca
     5101 tcggcgccaa caggaaactc ggcacgcgac tcctgcttat atgacagcac gatgtcgtcc
     5161 tgcagcgctt ggctcgcgtg acccgcccat gcgcgcacat ccggcatgcc gggcacgatg
     5221 gccgaagtcg tcaactcggg agggaccgga ttgcggatca gcgtgccgca gccggcggta
     5281 gcaaacagcg ccacgacaac gagcgcgccc gccctgcggc gaagacacgt tacgttggct
     5341 gccgcaccgg tgactgcctc actgtcgcgc atgatgaaag tgcggatccg gatcacgggc
     5401 accctcgtgg acaggccgat gtgtggctcg cgcggcacac ctctgcgtgc atgaacaggc
     5461 atgttcgttt gacttccgat tcgtcgaagc tagaatccgt cgcgattcag atgaagtcaa
     5521 gccttgggga agatgacagt ctgtcatcga ggtcgtcgcc ccgagctgga atgactaccc
     5581 cggactgatc actgcgctgc gccggctctg ccggcgcgct gcgtttctgt tcggtgcagt
     5641 tatcccgttt ccacgactgg cagactgagg cattgcacat gcgtaaagag ttttcgtccc
     5701 tgctgcgccg gtccgtgccg tcgcatgcct ttcgtccggc agcgattccg gttggcctgc
     5761 tgattgccgt ccttgcgctc gcgggctgcc gcaaggcagc tgaagcccct aaggaagaaa
     5821 tccggcccgt gcgcgtcgtg acgattgacc gcatcgccag cggagattcg gtcaacctga
     5881 ccggcacggt gcaggccgag accgaggtca acctgtcgtt ccgcatcgat ggtcgcatga
     5941 tcgagcggtc gatcggtgtc ggtgacgcgg tcaccgcggg aaaggtgatc gcccggctcg
     6001 atccttccaa cgagcagact gcacttcagt cagcgcgagc gacgctggcc ggcacacgtg
     6061 cgcagctagt cgaacagcgc aacaacttcc agcggcagaa agatctgctg gcgcagaatt
     6121 tcatatcgaa agccgcattc gatctgaccg cggccaacct gcagagtgcg gaatcggccg
     6181 cgcagtcggc gcagtcgcag gttgaccttg cgcagaaccg gctgagctac acccagctgg
     6241 tcgccgatgc gccgggcgtc gtgacggccg tcggcgcgga ggccggtgaa gtggtgccgg
     6301 ctgggcgcat ggtgatccag gtcgcgcgca agggcgggcg cgatgcggtg ttcgacgtgc
     6361 cggcgcaggt caaggacagc gcgccggcca accccgagat cacagtggtg ct
//
LOCUS       DATIRT010000323         4935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1820058, whole genome shotgun sequence.
ACCESSION   DATIRT010000323 DATIRT010000000
VERSION     DATIRT010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1820058"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..88)
                     /locus_tag="VLN25_07400"
     CDS             complement(<1..88)
                     /locus_tag="VLN25_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013211909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HSQ10425.1"
                     /translation="MMSVRAGNERGHADHGWLKSFHSFSFAEY"
     gene            215..1129
                     /locus_tag="VLN25_07405"
     CDS             215..1129
                     /locus_tag="VLN25_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HSQ10426.1"
                     /translation="MPTLTLDALQLIDAIARRGSFAGAADDLGRVPSAVTYAVRRLED
                     DLDVLLFDRRGYRARLTPAGEELLREGRHLLAAAEELTQRVRRVASGWEQELTLALDN
                     LIPFVGLVPLLDEFMRVAPTQLKITDEVLGGTWDALMSGRADLAIGAIQEGPEPSRLG
                     AGYRAEPLATVSFVFAVAPSHPLAAVGSPLPASELRKHRQVVVADTSQRMAPRAAGLL
                     GLADVLTVPNLAAKIAAQKAGLGCGFLPTHLVRDALARGELIAKTTDQERGDSTMHMA
                     WRNDARGKAVAWWLEKLRTSATRAALVA"
     gene            complement(1092..4220)
                     /locus_tag="VLN25_07410"
     CDS             complement(1092..4220)
                     /locus_tag="VLN25_07410"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HSQ10427.1"
                     /translation="MASLRPAPWFRAGGCIASAEFAIDTRDLTRGLSALRRAGSARTL
                     EGRFDLRVPFAVERRLLPVLLLVGLVAAASATAVGAPAFVWSWFALYAVNSAARFLLA
                     TTYGDARQPIPTDARCARQYLACAVADVLLWAALFAWVPNPAAFVAGPGAFAAAGGVL
                     LAALSFGGWPRVWTFYIAAWVGVLAVITARTTGTLTSFAFAFPLWLLAVWWLGRQQPL
                     GRHADRRAAQTLISNPTKFGWQAAIHAMPTPVIVARNGRIIEVNRSACEFIGRSERSI
                     LGCTLDECLIAEPPEALQPDKHRSESSASVDVRPADRTFAGSPWSGRVRYMEPGRVSS
                     VMVIALTKAAQEPFGPARLLEDARRFAEWIGGLQGLPWYRDERGALIVPREFPAPEEG
                     QRASDAAAFPLAYLLAAEDRDRVHALYREFLRTGTVFDERMALRDTQGVPREVRVVCV
                     VRPSGAESLAPVIGTIAAARPEAPRAPAEPVADLLSRLPVVVWLVDTSGRVVHATGPD
                     PRRWGMVAEARTRPEWHAAFAFKPESLPVVQEALQKALRGKPSFDVRNARTSRSGGRL
                     MLRSHFVPYEGKRMQAVLVMDTIASPFELLEMDRLKRSKGQYKSLVEASASLIWACDE
                     HFTITFASRRAARDIYGYETRELLGQPASVLLPTDAEQPSVRRAFDGLREGRSIRDLE
                     AVHITQEGRRVIVSISAVPLRHPGGRFAGAIGMNADLTALKSRETRLSEALRVERTVL
                     DSAGQAIAVVKQGLVLRCNDAFLRLAGLPPEQLARAPLNECLLEREDWDELTQAADEA
                     RSRDQAVVRELRVQCGARLGHGEGAAWCQITARSIAAGEFVLVITDIDMIKRREASAL
                     HEAQHDDLTGLPNRRLLAERAAAALATSELRKVSCAVLAIDLDGFKEINDRYGHEVGD
                     DMLREIAARLTRVMRPQDTVARRGGDEFAVLVPDVGSVLEVERIATRLLQSIKQPVAQ
                     QQGRPPLSVSASIGVSLAPEQGRDLERLLQLADLAMYDAKLKGKNRFAFAQGAGLPSN
                     VTPLVPRASRTS"
     gene            complement(4338..>4935)
                     /locus_tag="VLN25_07415"
     CDS             complement(4338..>4935)
                     /locus_tag="VLN25_07415"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HSQ10428.1"
                     /translation="AYILVLTDITSLKRREELAWHQANHDDLTGLPNRRQLGEHARRL
                     LSVALQKRRLAAVLVLDLDGFKEINDLFGHSYGDNLLKRVAQRLSGVLRDYDVVARTG
                     GDEFVVLLPEIEQPSIAMVVAEKLIAAASENVENHGRSLRLHASVGVALFPSDGHDFD
                     TLLAAADGAMYAAKAAGKNRYQLASEIVRTAAAPSRMS"
BASE COUNT          846 a         1725 c         1612 g          752 t
ORIGIN      
        1 gatattcagc aaatgagaag ctgtgaaacg acttgagcca gccgtgatcg gcatggccac
       61 gctcgttgcc cgctcgtacc gacatcattg aattcactcc tttcgttgag ccaaatccat
      121 tgaggatcgg caattcaagc cgagataatg aacgtataac cgagtaaaca catcgggatt
      181 agactgatga agtcattcga atccattcaa catcatgccg acgctgaccc tggacgccct
      241 ccagctgatc gatgcgatcg cccggcgcgg cagcttcgcc ggggcggccg atgatctggg
      301 ccgcgtacct tcggcggtca cttatgcagt gcgccggctc gaggacgatc tcgacgtcct
      361 gctgttcgac cggcgcggct atcgcgcccg cctgacgcct gcgggtgagg aactgctgcg
      421 cgaaggccgt cacctgctgg ctgccgcgga ggaactgacg cagcgcgtgc ggcgtgtagc
      481 ctcgggctgg gaacaggaac tcacgctggc actcgacaac ctcattccct tcgtcggact
      541 ggtcccgctg ctcgacgaat tcatgcgagt cgcgccgaca cagttgaaga tcaccgacga
      601 agtcctgggc ggaacctggg atgcgctgat gtcgggccgc gccgaccttg cgatcggcgc
      661 gatccaggaa ggcccggaac cgtcccgcct gggtgccggc tatcgggccg aaccgctcgc
      721 cacggtgagc tttgtgtttg cagtggcgcc cagccacccg ctggccgctg tcgggtctcc
      781 gctgccggcc agcgaactgc gcaaacatcg gcaggtcgtc gtcgccgaca cctcgcagag
      841 aatggcgcct cgtgccgccg gcctgctggg actggcggac gtgctgaccg ttccgaatct
      901 cgccgcgaag atcgccgcac agaaggccgg gctcggctgc ggcttcctgc ccacgcatct
      961 ggtgcgcgat gcgctcgcgc gcggcgaact gatcgcaaag acaaccgacc aggagcgcgg
     1021 cgacagcacg atgcatatgg cgtggcgcaa cgacgcccgc ggcaaggcgg tcgcgtggtg
     1081 gctggaaaag ctcaggacgt ccgcgacgcg cgcggcacta gtggcgtgac gttggagggc
     1141 agccccgcgc cctgtgcgaa cgcaaagcgg ttcttgccct tgagcttggc gtcgtacatc
     1201 gccaggtctg ccaactgcag cagccgttcg agatcccgcc cctgctccgg cgccagcgac
     1261 acgccgatgc tggcggacac gctgagcggc gggcggccct gctgctgtgc caccggttgc
     1321 ttgatcgact gcagcaaccg cgtggcaatg cgctccactt cgagcactga accgacgtcg
     1381 ggcacgagca cggcaaactc gtcgccgccg cggcgcgcga ccgtgtcctg cggccgcatc
     1441 acgcgagtca gacgcgcagc gatctcgcgc agcatgtcgt cgccgacctc gtgaccgtag
     1501 cgatcgttga tttccttgaa cccgtcgagg tcgatcgcca gcaccgcgca actgaccttg
     1561 cgcagttccg aggtcgcgag cgccgccgcg gcgcgttcag ccagcaggcg gcggttcggc
     1621 agcccggtca ggtcgtcgtg ctgcgcctcg tgcagcgcgc tcgcctcgcg gcgcttgatc
     1681 atgtcgatat cggtaatgac cagaacgaat tcaccggcgg cgatcgaacg cgcggtgatc
     1741 tggcaccagg cggcgccttc gccatggccg agcctcgcgc cgcactgcac gcgcaactcg
     1801 cgcaccacgg cctggtcgcg gctgcgcgcc tcgtccgcgg cctgcgtcag ttcatcccag
     1861 tcctcgcgtt cgagcaggca ttcattgagc ggtgcgcgcg cgagctgctc gggcggcaag
     1921 cctgccaggc gcaggaatgc gtcattacag cgcagtacca ggccctgctt gacgaccgcg
     1981 atcgcctggc cagcggaatc cagcaccgta cgttcgaccc gcagcgcctc ggacaggcgc
     2041 gtttcgcgcg atttgagcgc ggtcaggtcg gcgttcatgc cgatcgcgcc ggcaaagcgg
     2101 ccgcccggat ggcgcagcgg cacggcactg atcgacacga tgacgcggcg tccttcctgc
     2161 gtgatgtgga ccgcctcgag gtcccggatc gagcggccct cgcgcagccc gtcgaatgca
     2221 cgccgcaccg acggctgctc ggcgtcggtc ggcagcagga ccgatgccgg ttggccgagc
     2281 agttcccgtg tttcataccc gtagatgtca cgtgccgccc ggcgactggc aaaggtgatc
     2341 gtgaagtgct cgtcgcaggc ccagatcagg ctcgccgagg cttccaccag cgacttgtac
     2401 tggcccttgc tgcgcttgag tcgatccatt tccagcagct cgaacggcga agcaatcgtg
     2461 tccatcacga gcacggcctg catgcgcttg ccttcatagg gcacgaaatg gctgcgcagc
     2521 atcaggcgtc cgccggagcg gctggtgcgc gcattgcgga catcgaaact cggctttcca
     2581 cgcagcgcct tctgcagcgc ttcctgtacg acgggtaggg actccggctt gaaggcgaac
     2641 gcggcatgcc actccggacg cgtgcgcgcc tcggccacca tgccccagcg ccgcgggtcg
     2701 ggtccggtcg catgaacgac gcgcccggag gtatcgacga gccagacgac caccggcagt
     2761 cgcgacagca ggtccgcgac cggctcggcc ggcgcgcgcg gcgcctcggg acgtgcggcc
     2821 gcgatcgtgc cgatcaccgg cgcgaggctc tccgcgccgg acggacggac gacgcagacc
     2881 acgcgcactt cgcgcggtac tccctgcgtg tcacgcaggg ccatgcgttc atcgaacacc
     2941 gtccccgtac gcaggaactc acggtacagg gcatgcacgc gatcgcgatc ctctgcagcg
     3001 agcaggtagg ccagcgggaa cgccgccgca tcgctcgcac gctggccctc ctccggcgcc
     3061 gggaattcgc gcggcacgat gagcgcgccg cgctcgtcgc gataccacgg caaaccctgc
     3121 aggccgccga tccactcggc gaaccggcgc gcgtcctcca gcagccgcgc aggcccgaac
     3181 ggctcttgtg ccgccttggt cagcgcgatc accatgaccg aggagactcg tcccggctcc
     3241 atgtagcgca cgcggcccga ccagggcgag ccggcaaagg tccggtccgc cggccgcaca
     3301 tcgaccgacg cactcgactc ggagcggtgc ttgtccggct gcaaggcctc gggcggttcg
     3361 gcgatcaggc attcgtcgag cgtgcacccg aggatcgagc gttcgctgcg gccgatgaat
     3421 tcgcaggccg agcgattgac ttcgatgatg cgtccgttgc gagccacgat caccggcgtg
     3481 ggcatcgcat ggatggcggc ctgccagccg aacttggtcg gattgctgat cagcgtctgc
     3541 gccgcgcgtc gatcggcatg acgtccgagt ggttgctgcc gaccaagcca ccagacggcc
     3601 agcagccaca gcgggaacgc gaatgcgaag ctggtcagcg tgccggtcgt tcgcgccgtg
     3661 atgacggcca gcacaccgac ccacgcagcg atataaaagg tccagacgcg cggccagccg
     3721 ccaaagctca acgccgccag cagcacgcca cccgccgccg cgaaggcgcc cggccccgca
     3781 acgaaggccg ccggattcgg cacccatgcg aacagcgccg cccacagcag gacgtccgcc
     3841 acggcgcagg ccaggtactg gcgcgcgcaa cgcgcatcgg tcggaatcgg ctggcgcgcg
     3901 tcgccgtagg tcgtggccaa cagaaagcgc gctgccgaat tgacggcgta cagcgcgaac
     3961 cagctccaga cgaacgccgg cgctcccacc gccgtcgcac tggctgcagc gaccaggccg
     4021 acgagcagaa gtaccggcag cagcctgcgc tcgaccgcaa acggcacgcg caggtcgaac
     4081 cgaccttcaa gcgtgcgcgc cgatccggcg cgccgcaatg cgctgagtcc ccgggtcaga
     4141 tccctcgtat cgattgcaaa ctccgcactg gcaatgcacc cgcccgcgcg gaaccacggc
     4201 gccgggcgca gggaagccat gctagcggtc acgtgaaacc tacggcaatt gccagcagtc
     4261 ggccggccga acgttaatcc gaacggacga ggatggcgcg ctcgcgcccc gaggcgctcc
     4321 gcggccctcc gaggaactca gctcatccgg cttggcgcag cggccgtccg aacgatttcg
     4381 ctggcgagct ggtagcggtt cttgccagcg gccttggcgg catacatcgc gccatcggcc
     4441 gccgccagca gggtatcgaa gtcatggccg tcgctcggga acaaggcgac gccgacactc
     4501 gcgtgcagtc gcagcgagcg tccgtggttc tcgacgtttt cgctcgcggc cgcaatcagc
     4561 ttctcggcca cgaccatcgc gatcgacggt tgctcgattt cgggcagcag caccacgaac
     4621 tcgtcgccac ccgtgcgtgc caccacgtcg taatcacgca gcacgcccga cagccgctga
     4681 gcgacacgct tcaacaggtt gtcgccgtag gagtggccga acaggtcatt gatctccttg
     4741 aacccgtcca gatccagtac gagaacggcc gcgagccgcc gcttctgcag cgctaccgac
     4801 agcagacggc gggcgtgctc gccgagctga cggcgattcg gcagcccggt cagatcgtcg
     4861 tggttggcct gatgccaggc cagttcctcg cgccgcttca gcgaggtgat gtcggtcagc
     4921 accaggatgt aggca
//
LOCUS       DATIRT010000324         2068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_541121, whole genome shotgun sequence.
ACCESSION   DATIRT010000324 DATIRT010000000
VERSION     DATIRT010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541121"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VLN25_07420"
     CDS             complement(<1..446)
                     /locus_tag="VLN25_07420"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HSQ10429.1"
                     /translation="MGLINYITQVQFDFGAIRLARAECERSAIWRPLICTDKGVVAAG
                     LLDRLRESLGDLPVAVLDGTPSNPTEAAVMAAVEAYRAHQADGLIALGGGSAIDLAKG
                     VAIMATHLGTLADYATILGGSPKITPGVAPLIAIPTTAGTGSEVARG"
     gene            complement(599..>2068)
                     /locus_tag="VLN25_07425"
     CDS             complement(599..>2068)
                     /locus_tag="VLN25_07425"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HSQ10430.1"
                     /translation="TMYVIPFSMGPLGSHISQIGVEISDSAYVVVNMRIMTRMGRAVV
                     ELLGTDGAFVPCVHSVGKPLARGEKDVPWPCSSTKYIVQFPETREIWSFGSGYGGNAL
                     LGKKCLALRIASKMGHDEGWLAEHMLILGVTSPVGRKYHVAAAFPSACGKTNFAMLIP
                     PPAFAGWKVSTIGEDIAWIKPGKDGRLYAINPESGCFGVAPGTSEKTNYNAMAMLHEN
                     VIFTNVALTDEGDVWWEGMGPAPAHVIDWQGNDWTPEDGKAGRKAAHPNARFTVAAAQ
                     CPVIDADWEDPNGVPIDAFLFGGRRSTTVPLVTEARNWVEGVYMAATMGSETTAAAAG
                     AQGVLRRDPFAMLPFCGYNLGDHFGHWITLGHALRDAGATLPRIFTVNWFRTDADGKF
                     VWPGFGENMRVLQWIIDRVDGRADGAEHVFGVTPRYEDLNWNGLEFPRDRYEQITAID
                     AADWRKEIASHAELFDKLQPHLPSELDQTRAALARRVAA"
BASE COUNT          347 a          718 c          666 g          337 t
ORIGIN      
        1 ccgcgcgcca cttcgctgcc ggtaccggcg gtggtgggga tcgcgatcag cggcgcgaca
       61 cccggggtga tcttcgggct gccgccgagg atagtggcgt aatcggcgag cgtgcccagg
      121 tgcgtggcca tgatcgccac ccccttcgcg agatcgatcg cgctgccccc gccgagcgct
      181 atcagcccgt cggcctggtg cgcgcgatag gcctccacgg ccgccatcac cgccgcctcg
      241 gtcgggttcg acggcgtgcc gtctaacacc gccactggca ggtcgccaag cgactcgcgc
      301 agccgatcca gcagcccggc ggcgaccaca cctttgtcgg tgcagatcag cggacgccaa
      361 atggcgctgc gttcgcattc ggcccgcgcc agccggatcg cgccgaagtc gaactgcacc
      421 tgcgtgatgt agttgatcag gcccatgacg gactcctcag acggccacga ccgaacgcgg
      481 gcggacccgg gcaaacgggc agtatgtccg ctccgccaag tcggcgcaat tgcgaacgcc
      541 ccggagatga aaacgggccg ccagagcgac ccgtcgagag tactttcgtt gaaacggttt
      601 acgccgcgac ccgccgcgcc agggccgcac gcgtctgatc gagctcgctt ggtaaatgcg
      661 gctgcagctt gtcgaacagc tcggcgtgcg acgcgatctc cttgcgccag tccgcagcat
      721 cgatcgcggt gatctgctcg tatcggtcgc gcggaaactc caggccgttc cagttcaggt
      781 cttcgtaccg gggcgtgacg ccgaacacgt gctccgcgcc gtcagcgcgg ccatcgacac
      841 ggtcgatgat ccactgcagc acgcgcatgt tctcgccgaa gccaggccac acgaacttgc
      901 catcggcatc cgtgcggaac cagttgacag tgaagatcct gggcaaggtc gcacccgcgt
      961 cccgcagtgc gtggcccagc gtgatccagt gaccgaagtg atcacccagg ttgtagccgc
     1021 agaacggcag catggcgaac gggtcgcgcc ggagcacgcc ctgtgcgccg gccgccgccg
     1081 ccgtcgtttc ggagcccatc gtcgcggcca tgtacacgcc ctcgacccag ttgcgcgcct
     1141 cggtgacgag cggcaccgtg gtcgagcgcc ggccaccgaa caggaacgca tcgatcggaa
     1201 cgccgttcgg atcttcccag tcggcgtcga tcaccgggca ctgggccgcg gcgaccgtga
     1261 atcgcgcgtt cggatgcgcc gccttgcggc ccgccttgcc gtcctccgga gtccagtcgt
     1321 tgccctgcca gtcgatcacg tgagccggtg ccggccccat cccttcccac cagacgtcgc
     1381 cctcgtcggt cagcgccacg ttggtgaaga tgacgttctc gtgcagcatc gccatcgcgt
     1441 tgtaattggt cttctctgac gttcccgggg cgacaccgaa gcagcccgac tccggattga
     1501 tcgcgtagag gcggccgtcc ttgccgggct tgatccaggc gatgtcctcg ccgatggtcg
     1561 agaccttcca gccggcgaag gcgggcggcg ggatcagcat cgcgaaattg gtcttgccgc
     1621 aggccgacgg gaaggcggca gcaacgtggt acttccgccc taccggcgac gtgaccccca
     1681 ggatcagcat gtgctccgca agccagccct cgtcgtgccc cattttggac gcgatgcgca
     1741 gcgcgaggca cttcttcccg agcagcgcgt tgccgccgta gccggatcca aaggaccaga
     1801 tctcgcgcgt ctcaggaaac tggacgatgt acttggtgga actgcacggc cacggcacat
     1861 ccttctcgcc gcgcgccagg ggcttcccga cggaatgaac gcagggaacg aaggcgccgt
     1921 cggtgccgag cagttcgacg acggcgcgcc ccatgcgcgt catgatgcgc atgttgacga
     1981 cgacataggc gctgtctgaa atctcgacgc cgatctggct gatgtgcgac cccagcgggc
     2041 ccatcgaaaa tgggatcacg tacatcgt
//
LOCUS       DATIRT010000325         4250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_49428, whole genome shotgun sequence.
ACCESSION   DATIRT010000325 DATIRT010000000
VERSION     DATIRT010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49428"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="VLN25_07430"
     CDS             <1..857
                     /locus_tag="VLN25_07430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSQ10431.1"
                     /translation="VGYAGAAHDLTPLLAGAGAPPEVVSQLQSDLGERTRQLAGRSAE
                     LDHALRELDSFAYSVSHDLRAPLRVVDGFATIVLEDYGDRGKPLDDLGRDHLRRIVAA
                     SQRMNSMIETLLSLSRMTSRELARERVDLSQLARELVDDLRAQDHARAVEFVLAPDLR
                     ADGDSTLLRLVLQNLLGNAWKFTARGPQARIEFGTRDDGGVRTYFVKDNGAGFDMRFA
                     EKLFGLFQRFHSANEFPGTGVGLATVQKIIHRHGGRIWAEAVPAPNPGHGATFYFTLW
                     EGASGVRA"
     gene            complement(1252..1659)
                     /locus_tag="VLN25_07435"
     CDS             complement(1252..1659)
                     /locus_tag="VLN25_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10432.1"
                     /translation="MQAIDYIQRHGFKRWYERQLVESHAYLALGFVALILMLSGFEVM
                     GEMKSALGYASILGMAALGGLLMLVAWRRFNVLLARAERFAEGATCPTCNEWGKFRVL
                     GQESAPEDDPPEAGRPHWLRVRCSKCGGEWKIS"
     gene            complement(1670..2263)
                     /locus_tag="VLN25_07440"
     CDS             complement(1670..2263)
                     /locus_tag="VLN25_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSQ10433.1"
                     /translation="MMNEQQPLILVVDDDRLVLATLVAGLKQAGFEVIEADNGDDAIL
                     LARKYKPSLAILDMRMQGKSGMDVARYLAANTSTGFMFLSAFGDADIVEEATRMGALG
                     YLVKPLDVRQIVPAVRAALARWEELRVAPVIAPDSGGAPGRDEYIAIGILMERLRIDY
                     DRALEALRAQARAEGTTAAHLASTMVDAANRLNSIPR"
     gene            complement(2312..>4250)
                     /locus_tag="VLN25_07445"
     CDS             complement(2312..>4250)
                     /locus_tag="VLN25_07445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSQ10434.1"
                     /translation="EFELYMYRRKADPIWVAVQGRAVNPERPELGYIFAFVDIDERKR
                     SERELRSTLTELQLIFDNALVAMLYVSNDLILKANAATERLFGYDPRDYSELVVSSLF
                     ALPSDWEQVSAEAMEAGEDERYSFELLMRRADGSTFWCAGNGRPLEPGVPERGLILSL
                     MDVDARRRSEEELRRVRNYLDLVVENLPVLVSVREAETGRFVSLNRAGEQITGLSRHQ
                     VVGRTWREVYARQFADLYAELDRKALATGSQVDRPRDVMLRADGKRLTVNQRVVPLFE
                     AEPGVESHTRYVMSIIDDLTEEVRAEVALRETEARFRQFAENIDQLVFIMTGDFSSVL
                     YVNPRYQSLVGAPVEELVENPRHALKHVVSDDVPQLLRMLPRLVARMRRLLRSEFTVR
                     INHPTRGVCMISVRLNPVRMEDGAIRVFGVADDVTEREAAERQRMEEVVKQRDILVRE
                     VHHRIKNNLQGVAGLIQQAAVARPEVAAALEEAAAQIQAIAQVHGLQIRATGTLPVLG
                     VAQGIFQNLASMFGVEIRFEPPPPDLWRFGLPENEAVPLALVINELGTNAIKHRASRT
                     DVINVRVASRPDGMEFVIENPGQLKPGFSFADIAASVSGLGLVKALLPRRGAKLSIDP
                     AGSAVLARLQLFPPAVREENV"
BASE COUNT          714 a         1432 c         1382 g          722 t
ORIGIN      
        1 tcgtggggta cgcgggggcc gctcatgacc tcacgccgct gctggccggt gccggggcgc
       61 cccccgaggt ggtctcccag ttgcaatccg atctaggcga gcgcacacgc cagcttgccg
      121 ggcgctcggc cgaactcgac catgcgctgc gcgaactcga cagcttcgcc tactccgtct
      181 cgcatgacct gcgtgccccg ctgcgcgtcg tcgacggctt tgccaccatc gtgctcgagg
      241 actacgggga ccgcggcaag ccgctggatg acctgggtcg cgatcacctg cgccgcatcg
      301 tcgccgccag ccagcgcatg aactccatga tcgagacctt gctctctctc tcgcggatga
      361 cgagccggga actcgcgcgt gaacgcgtcg atctatcgca actcgcacgc gaacttgtcg
      421 acgacctgcg cgcccaggac cacgcgcggg cagtcgagtt cgtgttagca cccgacctgc
      481 gtgccgacgg tgacagcacg ctgctccgcc tggtgctcca gaatctgctc ggcaacgcgt
      541 ggaagttcac cgcccgcgga ccgcaggcgc gcatcgagtt cggaacccgc gacgacggtg
      601 gcgtgcgcac ctacttcgtg aaagacaacg gtgccggctt cgacatgcgc tttgccgaga
      661 agctcttcgg tctgttccag cgcttccaca gtgccaacga atttccggga accggcgtcg
      721 ggctggccac cgtgcagaag atcatccacc gccacggcgg ccgcatctgg gccgaggccg
      781 tccccgcgcc gaacccggga cacggcgcga cgttttactt cacgctctgg gaaggcgcct
      841 cgggtgtacg cgcgtaaggc gctcctcgtg tacgcgaacg ctacgcggtc gcacgtgcac
      901 tcagagcggc acgcgcgctg acgcgcgcgc ttaggtcact cggctgcgct ccggagcggc
      961 cgcgcgctcc gccgagtttc aactgcacag cactcaccct ggctctctcg ccgagcgaga
     1021 gggaatgtcg cactcctccc tgcaccctcc ttgcctggcg aggagggaaa ggcaacacgg
     1081 gcgcactggg agagagttcg cggggtggag tgcgcggggt cgtacctcgg cggagcgcgc
     1141 ggccgctccg gagcgcagcc gactagctag tgttgagcgg ttcgcgcaac atgcgaaccg
     1201 tatcgccgcg agctcacgtg cgacccggag ggtcgcgtaa gtgagcggcg cctagcttat
     1261 tttccactcc cccccgcact tcgagcagcg cacgcgcagc caatgcgggc ggccggcttc
     1321 gggagggtcg tcctcgggcg cggactcctg gccgaggacg cgaaacttgc cccactcgtt
     1381 gcaggtcggg caggtagcgc cttcggcgaa acgctcggcg cgagcgagca gcacgttgaa
     1441 ccgacgccat gccaccagca tcagcaaccc gcccagggcg gccatgccga gtatcgaggc
     1501 atagccgaga gccgacttca tctcgcccat cacttcgaag cccgatagca tcagaatcag
     1561 cgcgacgaag ccgagtgcga ggtaggcgtg gctttcgaca agttgccgct cataccagcg
     1621 tttgaagcca tgccgctgga tgtagtcgat ggcttgcact gcgcggtgat tagcgtggaa
     1681 tggagttgag gcggttcgcc gcgtcgacca tggtggaagc caggtgcgcc gccgtggtcc
     1741 cctcagcgcg ggcttgcgca cggagcgcct cgagcgcccg gtcgtagtcg atgcgcaacc
     1801 gttccatcag gatgccgatc gcgatgtact cgtcgcggcc gggcgcaccg ccgctgtccg
     1861 gcgcgatgac cggcgccacc cgaagttcct cccagcgtgc cagcgccgcg cgcaccgcgg
     1921 gaacgatctg gcgtacgtcg agcggcttga ccaggtagcc aagcgctccc atgcgggtgg
     1981 cttcctcgac gatgtcggca tcgccgaagg ccgacaggaa catgaatcct gtgctggtat
     2041 tggcagccag gtagcgtgcg acatccatgc ccgacttgcc ctgcatgcgc atgtcgagaa
     2101 tggccaggct gggcttgtac ttgcgcgcca gcaggattgc atcatcgccg ttgtcggcct
     2161 cgatcacctc gaaaccggcc tgcttaaggc ccgcgacgag ggtcgccagt acaagacggt
     2221 cgtcgtcgac gacgagtatc aggggttgct gttcgttcat cattgctcgc ttgggttcac
     2281 gtcgacggtg ctgaccgctc gtcaacgtga ctcacacatt ttcctcgcgt acggcaggcg
     2341 ggaacagttg cagccgcgcc agcacggcgc tgccggcggg atcgatcgac agttttgcgc
     2401 cccggcgcgg cagcagggcc ttgaccaggc cgagtcccga gacgctggcg gcgatgtccg
     2461 caaagctgaa ccccggcttc agctgtccgg gattttcaat cacgaactcc atgccatcgg
     2521 gccgtgaggc gacgcgaaca ttgatcacat cggtgcggct ggcgcggtgc ttgatcgcat
     2581 tggtcccgag ttcgttgatg accagcgcca gcggcacggc ctcgttttcc ggcagaccga
     2641 aacgccacag gtccggcggc ggcggctcga accggatctc gaccccgaac atactggcca
     2701 gattctggaa gatgccctgc gcaaccccga gtacgggcag ggtgccggtc gcgcggatct
     2761 gcagcccgtg cacctgtgcg atcgcctgta tctgagcggc ggcctcctcg agggccgcgg
     2821 cgacttccgg ccgcgcgacc gccgcctgct ggatcagacc ggccacgcct tgcaggttgt
     2881 tcttgatgcg gtgatggact tcacgcacca ggatgtcgcg ctgcttgacg acctcctcca
     2941 tgcgctgacg ctcggctgct tcgcgttcgg tcacgtcgtc ggcgacgccg aacacgcgga
     3001 tagcgccgtc ttccatccgc accggattga gccggacgct gatcatgcag acaccgcgcg
     3061 tcgggtgatt gattcggacc gtgaattccg agcgcaacag cctgcgcatg cgcgcgacca
     3121 gccggggcag catgcgcagc agctgcggca cgtcgtcact gaccacgtgt tttagcgcat
     3181 ggcgcgggtt ctcgaccagt tcctcgaccg gcgcgcccac cagcgactgg tagcgcgggt
     3241 tcacgtagag cacgctcgag aagtcacccg tcatgatgaa cacgagctga tcgatgttct
     3301 cggcaaactg gcggaagcgc gcttcggttt cgcgcaatgc gacctcagcg cggacttcct
     3361 cggtcaggtc atcgatgatc gacatcacgt accgggtatg cgattcaacg ccgggctcgg
     3421 cctcgaacag cggcacgacg cgctggttca ccgtcaggcg cttgccgtcg gcacgcagca
     3481 tcacgtcgcg cggccgatcg acctgggatc cggtcgccag tgccttgcgg tccagttcgg
     3541 cgtagagatc ggcgaactgg cgcgcataga cctcgcgcca ggtgcggccg acgacctgat
     3601 ggcgcgatag tccagtgatc tgctcgccgg cgcggttcag gctgacgaag cggccggttt
     3661 cggcctcgcg cactgacacc agcaccggca ggttctccac gacgaggtcg agatagttgc
     3721 gaacgcgccg cagctcctcc tccgagcgac ggcgcgcatc gacgtccatc agcgacagga
     3781 tcagaccgcg ctcgggtacg cccggttcga gtggccggcc attgccggcg caccagaagg
     3841 tgctgccgtc ggcacggcgc atcagcagct cgaacgagta gcgctcgtcc tcgcccgctt
     3901 ccatggcttc ggcactgacc tgctcccagt cggacggcag tgcaaagagc gaagaaacga
     3961 caagctcgga atagtcgcgc gggtcatagc cgaacagccg ctcggtcgcc gcgttggcct
     4021 tcaggatgag gtcgttcgac acgtacagca tcgcgaccag cgcgttgtcg aagatcagct
     4081 gcagttccgt cagcgtcgag cgcagctcac gctccgaacg cttgcgttcg tcgatgtcga
     4141 cgaaggcgaa gatgtagccg agttccggac gctccggatt gacggcgcgc ccctgcaccg
     4201 cgacccagat cggatcggcc ttgcgccggt acatgtagag ctcgaactcg
//
LOCUS       DATIRT010000326         6262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_345505, whole genome shotgun sequence.
ACCESSION   DATIRT010000326 DATIRT010000000
VERSION     DATIRT010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6262
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345505"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1261
                     /gene="gspE"
                     /locus_tag="VLN25_07450"
     CDS             <1..1261
                     /gene="gspE"
                     /locus_tag="VLN25_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HSQ10435.1"
                     /translation="PTKLTEAISQTYGDQSGSAQQIVGDIEGEFDITRMMQEVPDVED
                     LLETEDDAPIIRMINALLTQAARDGASDIHIEPFESYSLVRFRVDGTLRDVVRPKRAL
                     HAALVSRIKIMAALDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLEK
                     DLSKLELTNLGMAKGALDRFDTLIHQPHGIILVTGPTGSGKSTTLYAAIRRLDKNTTN
                     IMTVEDPIEYDIEGIGQTQVNPKIDMTFAKALRAILRQDPDVVMIGEIRDLETAQIAV
                     QASLTGHLVLATLHTNDAASAVARLIDMGIEPFLLSSSLLGVMGQRLVRKLCPKCRVQ
                     DSPTSPWRAVGCENCGNTGYVGRTGIYELLTVNDEMRMLVHQGANDTDIRRLAEKGGM
                     VNMRADAQRWVVAGVTSQEEALRVTRE"
     gene            1382..2629
                     /gene="gspF"
                     /locus_tag="VLN25_07455"
     CDS             1382..2629
                     /gene="gspF"
                     /locus_tag="VLN25_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HSQ10436.1"
                     /translation="MAAFRFAAADASGKEQTGVLEADSARVARQLLRGRGLIPLTVEP
                     VYGEAASGTGMAIRLGRRLSQTELAVITRQLASLMGAQLPVADALTVMVEQSEKQQVR
                     ELMASIRTDVLSGTSMSKALARHPRQFPEIYRALIAAGEESGKLGSVLGSLADYIEER
                     ARLQQKITLAFVYPAIVTLVALLVVVGLLTYVVPQVVQVFANTKQQLPFLTTALIAVS
                     DFVRNYGWIVVVALVIAAFLWERSLRVPAVRMRWHQRILTMPIFGVLSRTINTARFSS
                     TLAILVGSGVPMLRSLQAAGETVTNLAMRTRVMDAVQRVREGFSLAKALRADADEEKK
                     PGYIKLFPPVLIHLIASGEATGKLPEMLTRAADIHSREAERRALFFTSLLEPILILAM
                     GVVVMLIVLAVLLPIIEINTLVK"
     gene            2736..5342
                     /locus_tag="VLN25_07460"
     CDS             2736..5342
                     /locus_tag="VLN25_07460"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSQ10437.1"
                     /translation="MTELLEREPALTTLREALQGAATRGRVALVAGEAGIGKTSVLRA
                     LADEHAAQGPVWWGACDALQTPHPLAPLLDIAREARPRFASSVGGPRPALFDAVLDEL
                     RLAGAPVLVVIEDAHWADDATLDLLKFLGRRIERTRALLAVSFRDDEVTATHPLRRVI
                     GELPPAAITRVDLPRLTIQAVETLARRVSRPASGIYAATRGNPFFVTELLRDTGNTVP
                     RTVQDVVLARMSKLPAAAQELLRAVSVVPGSIERRLVDELLAPSLDSLEACLASGLLV
                     AESSTLSFRHELGRVAVESSLSTPVAQALHGRVLSALSVDGRNTAPAHLVHHAVRAND
                     TAAITRYAPVAAIQAASRSAHREAAAQWRIALKLGQPRDEAERCQWLEHAFVEFGTTA
                     ATNEAMQALDELQERARQRGDIAQAAYYLSRTANIHIGQLRGAEADAASREAIAMLAP
                     LPPSLTHAKVWQIEAWLRMLDRDYVDSVTWARRTIDTAEALGDALLANKARGGHGTAM
                     LFLDYGAGCAELEHVLETLLAHKDMAGAGVTLGNLGSGSGELMHLANAERWLREAVAL
                     CTAHELDNQLIYVSAWLALCTLLRGRWDEAGAVANEVLARTDEGNIARIMALLALGRL
                     RLRRGDPGADLVLDEARLLADRSKTLQRLAPTCCVRAEAAFGQGDLAAVAAEVAKALP
                     LAQSKGHPWFVGELAYWNWRAGVSKHAPDGCSPPYTLEIAGRWREAADAWFKLGCPYE
                     RARALSLGDTNAQQQALIIFDELGARPAADALRRQLREAGVRGVTRGARVSTRGNPAG
                     LTAAEMKVLGLMCRDLRNAEIAARLHRSVRTVDHHVAAVLAKLGVDTRLEAVRLAERE
                     GWVTPPDAASTI"
     gene            5412..5723
                     /locus_tag="VLN25_07465"
     CDS             5412..5723
                     /locus_tag="VLN25_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HSQ10438.1"
                     /translation="MAVRAADHPEILMPRYLIERTFPDGLSIPMNDDGRRVVAGVVAT
                     NADHGVTWVHSYVTTDRKQTFCIYDAPAPETIRKVAERNGLPIGRITEVSVLDPYFYR
                     P"
     gene            5748..6086
                     /locus_tag="VLN25_07470"
     CDS             5748..6086
                     /locus_tag="VLN25_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HSQ10439.1"
                     /translation="MKTPLLLFAVMLVSAPAFAQQRFVIEREIPGASKMTTDELRAAA
                     QKSNAVLRDLGPEIQWVQSYVAGDKIYCVYNAPSEAWIRSHAEKSGFPANRITPVSAV
                     IDPTTAAPKR"
BASE COUNT         1059 a         2136 c         2062 g         1005 t
ORIGIN      
        1 gccgaccaag ctcaccgaag cgatctcgca gacctacggc gaccagagcg gctcggcgca
       61 gcagatcgtc ggtgacatcg aaggcgagtt cgacatcacc cggatgatgc aggaggtccc
      121 ggacgtcgag gacctgctcg aaactgagga cgacgccccg atcatccgca tgatcaacgc
      181 gctgctgacg caggcggcgc gcgatggcgc gagcgacatc cacatcgaac cgttcgagtc
      241 gtattcgctg gtgcgcttcc gcgttgacgg cacgctgcgc gacgtcgtga ggccgaagcg
      301 cgcactgcac gccgcgctgg tgtcgcgcat caagatcatg gcggcactcg acatcgccga
      361 gaagcgcctg ccgcaggacg gccgcatcac tttgcgcgtt ggcggccgcc cggtcgatgt
      421 tcgcgtttcg acactgccca ccggccacgg cgaacgtgcc gtgctgcgtc tgctggaaaa
      481 ggatctgtcg aagctcgagc tgaccaacct cggcatggcc aagggcgcac tcgatcgctt
      541 cgacacgttg atccaccagc cgcacggcat catcctggtg accgggccga ccggctcggg
      601 caagtcgacg acgctgtatg cggcgatccg gcggctcgac aagaacacga ccaacatcat
      661 gacggtcgag gacccgatcg aatacgacat cgaaggcatc ggccagacgc aggtcaaccc
      721 gaagatcgac atgaccttcg ccaaggcact gcgcgcgatc ctgcgccagg accccgacgt
      781 cgtgatgatc ggcgaaatcc gcgacctcga gaccgcccag atcgcggtgc aggcctcgtt
      841 gaccggtcac ctggtgctgg ccaccctgca caccaacgac gcggccagcg ccgtggcgcg
      901 cctgatcgac atggggatcg agccgttcct gctgtcgtcc tcgctgctcg gcgtgatggg
      961 ccagcgcctg gtgcgcaagc tctgcccgaa gtgccgcgtg caggacagtc cgacgtcgcc
     1021 atggcgcgcg gtcggctgcg agaactgcgg caacaccggc tatgtcggcc gcacgggcat
     1081 ctacgaattg ctgacagtga acgacgagat gcgcatgctg gtccaccagg gcgcaaacga
     1141 caccgatatc cgccgcctcg ccgagaaggg cggcatggtc aacatgcggg ccgatgcgca
     1201 gcgctgggtc gtcgccgggg tgacctcgca agaagaagcg ctgcgagtca cgagagagta
     1261 attcgcagct cgtgtacgcg acgctgcgcg tcgcacgtgc actcgctgcg cctcagctag
     1321 cgcgccggtc ttcgggcggc cgagcgcccg gcgcgaatgc aaaggcaacg aagaggcaac
     1381 attggcagcg tttcgatttg cggcggcaga cgcctcgggc aaggagcaga ctggcgtact
     1441 cgaggccgat agcgcccggg tggcccggca attgctgcgc gggcgcggac tgattccgct
     1501 caccgttgaa ccggtctacg gcgaggcggc cagcgggacc ggcatggcga tccgcctcgg
     1561 ccgtcgcctg tcgcagacgg aactcgccgt catcacccgc cagctggcct cgctgatggg
     1621 cgcacagctg ccggtcgccg atgcgctgac cgtgatggtc gagcagagcg aaaaacagca
     1681 ggtgcgcgag ctgatggcgt cgatacggac cgacgtcctg tcgggaacgt cgatgtcgaa
     1741 ggcgctggcg cgccatccgc gccagttccc ggagatctac cgcgcgctga tcgcggccgg
     1801 cgaggaatcg ggcaagctcg gctcggttct cggcagcctc gccgattaca tcgaggaacg
     1861 cgcgcggttg cagcagaaga tcaccctggc cttcgtctac ccggccatcg tgaccctggt
     1921 cgcgctgctg gtggtcgtgg gtctgctcac ctatgtcgtg ccccaggtcg tgcaggtgtt
     1981 tgccaacacc aagcagcagc tgcctttcct gacgacggcc ctcatcgcgg tttcggactt
     2041 tgtccgcaac tatggctgga tcgtcgtcgt cgcactggtg attgcggcgt tcctgtggga
     2101 acgatcgctg cgcgttccgg cggtgcggat gcgctggcac cagcgcattc tgacgatgcc
     2161 catattcggc gtgctgtcgc gcacgatcaa cacggcgcgc ttctcgtcga cgctggcgat
     2221 cctggtggga tccggcgtgc cgatgctgcg ctcgctacag gccgccggcg aaaccgtgac
     2281 gaacctcgcg atgcgcaccc gcgtcatgga tgcggtacaa cgcgtgcgcg aaggtttctc
     2341 gctcgccaaa gcgctgcgtg ccgatgccga cgaggaaaaa aagcccggct acatcaaact
     2401 cttcccgccg gtgctgatcc acctgatcgc ctcgggtgaa gccaccggca agctgcccga
     2461 gatgctgacc cgcgccgcgg acatccattc acgcgaagcc gagcggcggg cgctgttctt
     2521 cacctcgctg ctcgagccga tcctgatact cgcgatgggc gtggtcgtca tgctgatcgt
     2581 gctggccgtg ctgctgccga tcatcgagat caacacgttg gtcaagtagc cccgccgcga
     2641 ccggccggcg ggtaaatgcc ggtcattgcg cgaaccgcgc ctcgatacac tggcgcgccc
     2701 gcattgccgc cgggtcggcg cgccggccca cccgcatgac tgaactgctc gaacgagagc
     2761 cggcactcac cacgctgcgc gaagcgctgc agggcgcagc cacgcgcgga cgggttgccc
     2821 tggtcgccgg cgaggccggc atcggcaaga ccagcgtgct gcgcgcgctg gccgatgaac
     2881 atgcagcgca gggtccggtc tggtggggag cgtgcgacgc actgcagacg ccgcaccccc
     2941 tcgccccgct gctcgacatc gcgcgcgaag ctcgcccccg cttcgcgtcg tctgtcgggg
     3001 gtccgcgccc ggcgctgttc gacgctgttc tcgacgaact gcgcctggcc ggcgcgcccg
     3061 tgctggtcgt catcgaagat gcgcactggg ccgacgacgc gacactggac ctgctgaagt
     3121 ttctcggccg ccgcatcgaa cgcacgcggg cactgctggc cgtgagcttc cgcgacgacg
     3181 aggtcaccgc cacgcaccct ttgcgccgcg tgatcggcga actaccgccg gccgcgatca
     3241 cacgcgtcga cctgccgcga ctcacaatac aggcggtcga aaccctcgcc aggcgggtgt
     3301 cgcgtccggc gtccggcatc tacgctgcca cgcgcgggaa tcccttcttt gtgaccgagt
     3361 tgctgcgtga caccggcaat accgtgccgc ggaccgtgca ggacgtcgtt ctggcacgca
     3421 tgtcgaagtt gccggccgcg gcacaggagt tgctgcgcgc cgtctcggtc gttcctggaa
     3481 gcattgaacg acggctggtc gatgagctgc tcgcgccctc gctcgacagt ctcgaggcct
     3541 gcctggcgag cggcctgctg gtggccgaga gctcaaccct cagcttccgg catgagctcg
     3601 gtcgcgtcgc cgtcgaatcc tcgctctcga ctccggtcgc gcaggcgctg cacggtcgcg
     3661 tgctgtcagc gctgtcggtc gacggccgca acactgcgcc cgcgcacctg gtccatcacg
     3721 cagtccgcgc caacgacacc gcggccatca cccgctacgc accggtggca gcgatccagg
     3781 ccgcttcgcg cagcgcacac cgcgaggcgg ccgcacagtg gcgtatcgcg ctgaagctgg
     3841 ggcagccgcg cgatgaggcc gagcgctgcc agtggctgga gcatgcgttc gtcgagttcg
     3901 gcaccactgc ggcgacgaac gaggcgatgc aggcgctcga cgaacttcaa gagcgggcac
     3961 gccagcgagg cgacatcgcg caggccgcgt actacctcag tcgcacggcc aacatacaca
     4021 tcgggcaatt gcgcggtgcc gaggccgatg cagcgtcgcg cgaggccatt gcgatgctcg
     4081 cgcctctgcc accgagcctc acgcacgcca aggtgtggca gatcgaggcg tggctgcgca
     4141 tgctcgaccg tgattacgtc gacagcgtca cgtgggcccg ccggactatc gacaccgccg
     4201 aagccctggg cgacgctctg ctcgccaaca aggcgcgggg cgggcacggc acggccatgc
     4261 ttttcctgga ctacggagcg ggttgcgccg agttggagca cgtactggag acgctcctcg
     4321 cccacaagga catggcgggc gccggcgtaa cgctgggcaa cctaggctcg ggctcgggcg
     4381 aactgatgca cctggccaat gccgagcgct ggctgagaga agccgttgca ctctgtacgg
     4441 cacacgagct ggacaatcag ctcatctatg tcagcgcgtg gcttgcgctc tgcaccctgc
     4501 tgcgtggccg ctgggacgag gcgggtgctg tcgccaacga ggtgctggcc cgcaccgacg
     4561 aaggaaacat tgcgcggatc atggccttgc tggccctggg ccggctgcga ttgcggcgcg
     4621 gcgacccggg cgccgacctc gtgctcgatg aagcgcgtct cctggccgat cgcagcaaga
     4681 cgctgcagcg cctggccccg acctgctgcg tccgggccga agctgcgttc ggacagggcg
     4741 acctggccgc cgtcgcggcc gaggtcgcca aggcgctgcc gctggcgcaa tccaagggac
     4801 atccgtggtt tgtcggcgaa ctggcctact ggaactggcg cgcgggcgtg agcaagcacg
     4861 ctcccgacgg ttgctccccg ccttacacac tggaaatcgc cggccgctgg cgcgaagctg
     4921 cggatgcctg gttcaagctt ggctgcccct acgaacgcgc ccgcgcactg agcctcgggg
     4981 acaccaacgc acagcagcag gcgctgatca tcttcgatga gctcggcgcc cgccccgccg
     5041 ctgacgcctt gcgccgccag ctacgcgagg ccggtgttcg cggcgttaca cgcggcgctc
     5101 gggtttcgac ccgaggcaac ccggccggcc ttacggccgc cgaaatgaag gtgctcgggc
     5161 ttatgtgccg cgacctgcgc aatgccgaga tcgcggcgcg actgcaccga tcggtgcgca
     5221 cggtcgacca ccatgtggcc gcggtgctgg ccaagctcgg cgtcgacacg cgcctggaag
     5281 ccgtgcgact cgccgagcgt gaaggctggg tgacgccgcc ggatgcggcc tccacaatct
     5341 aggcaatcgt tcgccgaaga ataggcaacg ctgccgacgc caggcagcgc ggggatcttc
     5401 aaagtgcggt catggcagtg cgtgctgccg atcacccgga gatcctgatg ccccgctatc
     5461 tcatcgaacg aacttttccc gacggcctgt cgatcccgat gaacgacgac ggtcgccgag
     5521 tcgtcgctgg tgtggtcgcc accaacgccg atcatggcgt gacctgggtc cattcatacg
     5581 tgacgacgga ccgcaagcag accttctgca tctacgacgc gccggcgccg gaaacgattc
     5641 gcaaggtcgc ggaacgcaac ggactgccga tcggccgcat taccgaagtc agcgtgctcg
     5701 acccgtattt ctaccgtccc tgaccctcac gcgaaccgga gctgcacatg aaaacccccc
     5761 tccttctgtt cgctgtcatg ctggtatccg cacccgcctt cgcccagcag cgcttcgtga
     5821 tcgaacgtga aattcccggt gcctcgaaga tgacgacaga cgagctgcgc gccgccgcac
     5881 agaagtccaa cgcggtgctg cgggacctgg gtccggaaat tcagtgggtg cagagctacg
     5941 tggctggcga caagatctac tgcgtctata acgctccgag cgaagcctgg atccgcagcc
     6001 atgccgaaaa gtcgggcttc ccagcgaaca ggatcacgcc ggtgtccgcg gtcatcgacc
     6061 cgacgacagc cgcgccaaaa cgctaggtca ttcctggcgg cgcggtcagc gcactgggag
     6121 tgtccggcgg caggtcgtca cggaggcccg ccgccggaat ctcacagctt gtatctcacc
     6181 gcttatgtca acgcctgcag tttgtcgtag gtggccttgt cccaggtcgc gtcgggtccg
     6241 ttgtgcattt tggcaaccga tt
//
LOCUS       DATIRT010000327         2084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_935379, whole genome shotgun sequence.
ACCESSION   DATIRT010000327 DATIRT010000000
VERSION     DATIRT010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935379"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /gene="glmM"
                     /locus_tag="VLN25_07475"
     CDS             complement(<1..1106)
                     /gene="glmM"
                     /locus_tag="VLN25_07475"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HSQ10440.1"
                     /translation="MTRKYFGTDGVRGTVGETPITPEFVLRLGQAAGRVLARRAEGGT
                     TRPTVLIGKDTRISGYMLEAALEAGFSSAGVDVVLCGPLPTPAVAYLTRALRLDAGIV
                     ISASHNPYPDNGIKFFSERGTKLPDAVEERIEQELDQPWHCVDARQLGKVRRLDDAAG
                     RYIEFCKRTFPNDFDLKGLKLVVDSANGAAYHVAPHVFHELGADVVSHGASPNGLNIN
                     DGVGALHAEQLAHAVQVAEADLGIALDGDADRVLICDADGRLYNGDELLYVIVRDRMR
                     VGKVAGAVGTLMTNYALERRFSELGVGFARAKVGDRYVLEMLEEKGWQYGGEGSGHLL
                     CLDCHSTGDGIVGALQVLAAMKRSGRTLAQLTSDL"
     gene            complement(1103..1882)
                     /gene="folP"
                     /locus_tag="VLN25_07480"
     CDS             complement(1103..1882)
                     /gene="folP"
                     /locus_tag="VLN25_07480"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HSQ10441.1"
                     /translation="MGIVNVTPDSFSDGGRYLSAPAALEHARRLIDEGADMLDIGGES
                     TRPGAADVSEPEEIERVVPLIEALRSTSVPLSVDTSKPGVMRAALVAGASIVNDVRAF
                     LEAGAPEAVVGSRCGLVLMHMQGTPRTMQREPSYGNVVQEVSDFLRSRVAALVHVGVA
                     AERIAVDPGFGFGKSVAHNFRLLRELDALTALGRPVLVGLSRKSMLGAVTGRAVGERI
                     AASVAAAVLAAERGARIVRVHDVAATRDALRVWAAMRKDEG"
BASE COUNT          356 a          743 c          663 g          322 t
ORIGIN      
        1 aggtccgagg tcagctgggc cagtgtccgg ccgcttcgct tcatcgcggc cagcacctgc
       61 agcgcaccga cgatgccgtc tccagtgctg tgacaatcga ggcacagcag gtggccggaa
      121 ccctcgccgc cgtactgcca gcccttctcc tccagcattt ccaggacgta gcggtcgccg
      181 accttggccc gcgcgaagcc gaccccgagt tcggaaaagc ggcgctcgag cgcgtagttc
      241 gtcatcagcg tgccaacggc gcctgccacc ttgcccacgc gcatgcggtc gcgcacgatc
      301 acgtacagca gttcatcgcc gttatagagg cgcccatcgg cgtcgcagat cagcacgcga
      361 tcggcatcgc cgtcgagggc gatgccgaga tccgcctcgg ccacctgcac cgcatgcgca
      421 agctgctcgg catgcagggc gccgacgccg tcgttgatgt tcagcccgtt gggcgacgcg
      481 ccgtgcgaga cgacatcagc gccgagttcg tggaacacgt gcggagcgac gtgataggca
      541 gcaccgttcg cactgtcgac gacgagcttt agtcccttga gatcgaagtc attcgggaac
      601 gtgcgtttgc agaactcaat gtaacggccc gcggcatcgt cgagacgtcg caccttgccg
      661 agctggcgcg cgtcgacgca gtgccacggc tggtcgagtt cctgttcgat gcgttcctcg
      721 acggcatccg gcagcttggt gccgcgctcg gagaaaaact tgatgccgtt gtccggatac
      781 ggattgtgcg aggcgctgat gacgatgccg gcgtcgagcc gcagggcccg ggtgaggtaa
      841 gccacggccg gtgtcggcag cggtccgcac aacacgacgt cgacaccggc actcgagaag
      901 ccggcttcga gcgcggcctc gagcatgtaa cccgagatgc gtgtgtcctt gccgatcagt
      961 acggtcggtc gggtggtccc gccttcggcc cgccgcgcca ggacccgccc ggccgcctgc
     1021 ccgagccgca gcacgaactc cggcgtgatc ggcgtttcac ccacggttcc gcgcactccg
     1081 tcggtaccga aatacttgcg cgtcatccct cgtcctttct catcgccgcc cagacccgca
     1141 gcgcgtcccg cgtggctgca acatcatgta cccgcacgat acgcgcgccg cgctccgccg
     1201 cgagcacagc ggcggccacc gaggccgcga tgcgctcgcc gaccgcccgg cccgtcaccg
     1261 cgccgagcat cgacttgcgc gacagtccga ccagaacggg cctgccgagc gccgtcagcg
     1321 cgtccagctc gcgcagcagg cggaaattgt gcgccaccga cttgccgaaa ccgaatcccg
     1381 gatcaacggc gatgcgttcg gccgcaacac cgacatgcac gagtgcggcc acgcgggacc
     1441 gcaggaagtc gctgacttcc tgtacgacgt tgccatagct cggctcccgt tgcatcgtcc
     1501 gcggcgtgcc ctgcatgtgc atcagaacga ggccgcagcg gctccccacg acggcctcgg
     1561 gcgcccccgc ctccaggaaa gcgcgcacgt cgttgacgat tgacgcgccg gccaccagcg
     1621 cagcccgcat cacgcccggc ttgctggtgt cgaccgacaa cggcaccgaa gtcgagcgca
     1681 gcgcctcgat cagcggcacc acgcgttcga tctcctcggg ctccgatacg tcggccgcac
     1741 cgggccgcgt ggactcgccc ccgatgtcga gcatgtccgc accctcgtcg atcaggcggc
     1801 gcgcgtgctc cagagccgcc ggtgccgaca ggtaccggcc gccgtccgaa aacgaatcgg
     1861 gggtaacgtt gacgattccc atcacaagcg gccgcgacaa atcgagatcg cggcccccga
     1921 ccgaccacgc gggtgtcgat cggacgacgg gactgactgc gggactgact gcgggactcg
     1981 ctgctggatc gcctacgctg ctcattgttt aattgtagaa gcgcccaaga aaaaggccgg
     2041 acgagccggc ctttgatgca gggaacgtgg cgactacgcc ggta
//
LOCUS       DATIRT010000328         7450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1427893, whole genome shotgun sequence.
ACCESSION   DATIRT010000328 DATIRT010000000
VERSION     DATIRT010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7450
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427893"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /gene="mnmD"
                     /locus_tag="VLN25_07485"
     CDS             complement(<1..743)
                     /gene="mnmD"
                     /locus_tag="VLN25_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HSQ10442.1"
                     /translation="MSLQPARLAREADGTPYSERYGDVYASRDGAVGQAQHVFLGGVG
                     LPDAWHGRDQYVVLETGFGLGSNFLVTWQAWRADPQRPRRLHFVSIEKYPLAAADLLA
                     AASDCLRPLAGALARQWPLAVAGLHRLEFDGGGVCLTLALGDARDLLPQLVLGADAIF
                     LDGFAPGRNPEMWEPALLKAIGRCARPGAALATYTTAKSVRAALEAAGFDLHVRPGYA
                     SKREMLCGRYAPRWRVRRHEPPMAYGGVRS"
     gene            801..2630
                     /locus_tag="VLN25_07490"
     CDS             801..2630
                     /locus_tag="VLN25_07490"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HSQ10443.1"
                     /translation="MFTRLLLCLSLVLLMDPTYAQPRPRIEKAADLPRFTYPVKGSLE
                     ALVKDPQAFGGFSANVRRDIESVLAKYDIADKSSERQLLGTLLQLDLLEGRDADALKR
                     IEQIRALQEKPADKLLTGLQARAIVDGRKAAGSTSGPAYLAAAAGSLTAALKPMPYPV
                     IANDIKEAKASAELIGEGRVLGYINEVLQPTADKTGALSSDLAPGVIGARYTLQLRLP
                     LKQTLVDTYSAYLAANKVEKPDIWAARGVALEPGRPYTPVVVAVWDSGVDTAIFKSQL
                     VRDAKGAPALIGFDKYANPTNTELQVLTPAMQARLPAMKSRSKGFSDLQSNVDSPEAN
                     EVKQLISTLPREQYKPTIEELMLAGNYTHGTHVAGIAMEGNPYARLLVARMEFGNTLI
                     PDPCPSLEQTRKDAKSFVETVEFLRRNKARVVNMSWGGSVNAIENDLELCGIGKTQDE
                     RQKLAREYFSIIKAALEKAFAAAPEILFITAAGNSNQNASFAEDAPADIVLPNLLTVG
                     AVDKAGDEASFTSYGPTVKVHANGYQVESFLPGGDRVAYSGTSMAAPQVANLAGKILA
                     VNPALTPTQVIEIIVSTADKTADGRRTLVNPKKALEGARQKKA"
     gene            2635..3384
                     /gene="alkB"
                     /locus_tag="VLN25_07495"
     CDS             2635..3384
                     /gene="alkB"
                     /locus_tag="VLN25_07495"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HSQ10444.1"
                     /translation="MRLPSVDAARLFLDPIRSFSPAVFAHAVGAKREGVKLGSDLFDL
                     ESRTIALAPGAMVLRNFAAADAVALLAGVADIEQCAPFRHLITPGGFRMSVAMTNCGA
                     VGWVSDRTGYRYDREDPDTGLEWPSMPSTFRRAAVAAAAAAGFDRYEPDVCLINRYEP
                     GARLSLHQDRNELDDEAPIVSVSLGLPAVFQFGGLKRSDRCARVPLEHGDVVVWGGPS
                     RMRFHGVLPLAEGCHPELGRKRINLTFRTAR"
     gene            complement(3486..5153)
                     /gene="ettA"
                     /locus_tag="VLN25_07500"
     CDS             complement(3486..5153)
                     /gene="ettA"
                     /locus_tag="VLN25_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HSQ10445.1"
                     /translation="MAQYVYTMIRVGKIVPPKREILKNISLSFFPGAKIGVLGLNGSG
                     KSTLLRIMAGLDTDIEGEAVPMPGLSIGFLPQEPQLDPAKTVKDEVEAGLGEVMQAKQ
                     KLEEIYAAYAEPDADFDKLSADQAKYEAIIATSGADVEHQMEIAADALRLPPWDASIA
                     KLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVDWLEQFLSRFPGTVVAVTHDR
                     YFLDNAAEWILELDRGMGIPWKGNYSSWLEQKEERLEREAKSEVARIKTMKNELEWVR
                     QNAKGRQAKSKARLKRFDELSSYEYQTRNETQEIFIPVAERLGNQVIEFKNVSKGYGD
                     RLLIDSLSFAVPPGAIVGVIGPNGAGKSTLFHMITGQEKPDAGEIVMGPSVQIAAVEQ
                     TRESLPNDKSVWEAISGGLDILQVGRFEMPSRAYLGRFNFKGGDQQKIVGNLSGGERG
                     RLHLAATLLKGGNVLLLDEPSNDLDVETLRALEEALLEFAGSVLVISHDRWFLDRIAT
                     HILAAEGDSQWTFFNGNYQEYEADKKKRLGEEGAQPHRLRFKPLTRV"
     gene            complement(5207..7189)
                     /locus_tag="VLN25_07505"
     CDS             complement(5207..7189)
                     /locus_tag="VLN25_07505"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HSQ10446.1"
                     /translation="MTLLTPWRAILVRAFKRLFPGRFAVAGIVVVAYLAVSLLTRVGL
                     AIFNNESAEFMPWRVAGWLLIGLTYDLAAATFWLLPIALLAWLWPADRARRAFGIAAI
                     VLATIAFGIFVFTAVSEFVFWNEFSSRFNFIAVDYLIYTREVIGNIRESYRMTPILLG
                     LAGATAVVVALATRPIWRTAQAPGPAFGRRSLQMAGYIAVALASYLLVPSTWKEFSSS
                     VQSQQLAGNGHWEFFHAFNSNEIDYDRFYATVPIEQAYKELREEFTDAGPVEFTGDAG
                     LPIERVIRGAAPEKPLNVVLISVESLSADYMGAFGNAKGLTPQLDRLALEGLLFTRLY
                     ATGTRTVRGLEALALSLPPTPGHSVVKRPNNDHLFTVGEVFAEKGYEPLYVYGGYCYF
                     DNMEHFFGGNGYTVIDRTALDKADIHYENIWGVADEDLFSLTVRELDKRHIAGKKFFA
                     HVMTTSNHRPYFYPAGRIDIAPKSGADGAVKYTDWAIGDFIERARKHAWFDDTVFVIV
                     ADHCAASRGKTDLPLDRFHIPLLIYAPKHIAPARIDTIASQIDVAPTVLALLNFNYVS
                     RFFGQDILNEGRTHQRAMMANYQTVGYYEHGMLVELRPQRRVRIIDAASGRELPPSDE
                     ARHFTNEAIGYYQGASNAFRTGALRMPGHLLPRPVK"
     gene            7329..>7450
                     /locus_tag="VLN25_07510"
     CDS             7329..>7450
                     /locus_tag="VLN25_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HSQ10447.1"
                     /translation="MTAFLISIGVVALAEIGDKTQLLALALAAKYRKPWPIIFG"
BASE COUNT         1385 a         2392 c         2371 g         1302 t
ORIGIN      
        1 gaacgcactc ccccgtaagc catcggaggc tcatggcggc ggacacgcca gcgcggcgcg
       61 tagcggccgc acagcatttc gcgcttgctg gcgtagcccg gccgcacgtg cagatcgaag
      121 cccgcagctt ccagagccgc acggacggac ttagcggtcg tatacgtcgc caacgcggcg
      181 cccgggcggg cacaacgccc gatcgccttg agcagcgcgg gctcccacat ctcgggattg
      241 cgaccggggg caaatccgtc gaggaagatg gcatccgcac caaggacgag ttgcggcagc
      301 aggtcgcgcg cgtcgcccag cgccagtgtc aggcagacac cgccgccgtc gaattccagc
      361 cggtgcagcc cggccactgc cagcggccat tggcgggcaa gcgccccggc cagcggccgg
      421 aggcagtcgg atgccgccgc gagcagatcc gctgcagcca gcgggtactt ctcgatcgaa
      481 acaaaatgca gacgccgcgg tcgctgcggg tctgcgcgcc aggcctgcca ggtgacgaga
      541 aaattgctgc ccagcccgaa gccggtttcg agcaccacat actggtcgcg cccgtgccag
      601 gcgtcgggca ggcccacgcc cccgagaaag acgtgctgtg cctggccaac cgcgccgtcg
      661 cggctggcgt acacgtcgcc atagcgctcg ctgtacggag tgccgtccgc ctcgcgggcg
      721 agccgtgccg gttgcaggga catgcggaat tatcggcgcg gcgcagctaa cattcgaccc
      781 tttcccgccc ggactccgcc atgttcacgc gcctccttct ctgcctcagt ctcgtactac
      841 tgatggaccc gacctatgcc cagccgcggc cgcgcatcga aaaggccgcg gacctcccgc
      901 gcttcaccta tcccgtcaag ggaagcctcg aagctcttgt caaggacccg caggcgttcg
      961 gggggttcag cgccaatgtc cgccgcgaca tcgagtccgt gctggccaaa tacgacatcg
     1021 ccgacaaatc gagcgaacgg cagctgctgg gcacgctgct gcagctcgac ctgctcgagg
     1081 gccgcgatgc cgatgcactc aagcgcatcg agcagatacg ggcgctgcag gaaaaacctg
     1141 cggacaagct gctgaccgga ttgcaggcgc gcgcgatagt tgacggccgc aaggctgcgg
     1201 gcagcaccag tggaccggct tatcttgcgg ccgccgccgg cagcctgacc gcggcgctca
     1261 agccgatgcc ctacccggtc attgccaacg acatcaagga agccaaggcc agcgccgagc
     1321 tgatcggcga agggcgagtg ctcggctaca tcaacgaggt gctgcagccg accgccgaca
     1381 agaccggcgc actgagcagc gatctcgcgc cgggcgtcat cggagcgcgc tatacgttgc
     1441 agctgcgact gccgctcaag cagacgctgg tggacaccta cagtgcctat ctggccgcca
     1501 acaaggtcga aaagcccgat atctgggccg cgcgcggcgt tgcgctcgaa ccgggccgtc
     1561 cctatacgcc ggtcgtcgtc gctgtctggg acagcggcgt cgataccgca atcttcaagt
     1621 cgcagctcgt gcgcgacgcc aaaggcgcgc cggcgttgat cggcttcgac aagtacgcca
     1681 atccgaccaa caccgaactg caggtgctga cgccagccat gcaggcgagg ctgccggcaa
     1741 tgaagtcgcg cagcaaggga ttctcggacc tccagtccaa cgtcgacagt ccggaagcca
     1801 acgaggtcaa gcagctgatt tcgactttgc cgcgcgagca gtacaagccg acgatcgagg
     1861 aactgatgct ggccggcaac tacacgcacg gaacgcacgt ggccggcatc gcgatggaag
     1921 gtaatcccta cgcgcgcctg ctggtggcgc gcatggagtt cggcaacacg ctgattcccg
     1981 atccctgtcc gtcgctcgaa cagacgcgca aggatgcgaa gagctttgtc gaaaccgtgg
     2041 agtttctgag gcgcaacaag gcgcgcgtcg tcaacatgag ctggggtggc agcgtcaacg
     2101 cgatcgagaa cgacctcgaa ctgtgcggca tcggcaagac gcaggacgag cgccagaagc
     2161 tggctcgcga gtatttcagc atcatcaagg ccgccctgga aaaggcattc gcggcagcgc
     2221 ccgagatcct gttcatcacg gccgccggca actccaatca gaatgccagc ttcgcggaag
     2281 acgctcccgc cgacatcgtg ctgcctaatc tgctgacggt cggtgcggtc gacaaggccg
     2341 gcgacgaagc ctcgtttacc agctacggtc cgaccgtcaa ggtgcatgcc aatggttacc
     2401 aggtcgaaag cttcctgccc ggcggcgatc gagtcgctta ctccggcaca tcgatggcgg
     2461 caccacaggt cgccaacctc gccggcaaga tccttgcggt caacccggcg ttgacgccaa
     2521 cgcaggtcat cgagatcatc gtctcgacgg ctgacaaaac ggccgatggt cgccgcacgc
     2581 tggtcaatcc gaagaaagcg ctcgaggggg cgcgacagaa gaaggcgtaa ggcagtgcgc
     2641 ttgccgtcgg ttgatgctgc aagactcttc ctggacccga tccgcagctt ttcgccggca
     2701 gtgtttgccc atgcggtcgg agcgaagcgc gaaggggtga agctgggctc ggatctgttc
     2761 gacctcgaat cacggaccat tgcgcttgca cctggagcaa tggtcttgcg caacttcgca
     2821 gcagcggacg ccgtggcctt gctcgccggt gtcgccgaca ttgagcagtg cgcaccgttc
     2881 agacacttga tcacccccgg cggcttccgc atgtcggtcg cgatgaccaa ttgcggcgcg
     2941 gtcggctggg tatcggatcg gacgggttat cgctacgacc gcgaggatcc ggacacaggc
     3001 ctcgagtggc cgtcgatgcc atcgactttc cggcgcgctg cagtcgccgc tgcagccgcg
     3061 gccgggttcg atcggtacga gccggatgtc tgcctgatca atcgctatga gccaggggca
     3121 cggttatcgc tgcatcagga tcgcaacgaa ctcgatgacg aagccccgat cgtttccgtc
     3181 tcgctcggct tgcctgcggt cttccagttt ggcgggctga agcggtccga ccggtgtgca
     3241 cgtgtgccgc tggaacatgg tgacgtcgtt gtctggggcg gaccctcacg catgcgattt
     3301 cacggcgtac tgccgcttgc cgaggggtgt catccggagc tgggccgcaa gcgcatcaat
     3361 ctcaccttcc gcactgcacg ctgagtcggc ccagcggacg cgaacaactc gacccaatgg
     3421 tcacggccgt cgcgtttgtc cgcgggtgga cccgcccgac gcgccagcgg ccgactgagc
     3481 acgcctcaga cgcgcgtcag cggcttgaaa cgcagccggt gcggttgtgc gccctcttca
     3541 cccaggcgct tcttcttgtc ggcttcgtat tcctgatagt tcccgttgaa gaaggtccac
     3601 tgcgagtcgc cttcggcggc gaggatgtgc gtggcgatgc ggtcgaggaa ccagcggtcg
     3661 tgcgagatca ccagcaccga gcctgcaaac tcgagcagcg cttcttccag cgcgcgcagc
     3721 gtctcgacat cgaggtcgtt tgaaggctca tcgagcagca gcacgttgcc gcccttgagc
     3781 agggtcgccg ccagatggag gcgccctcgt tcaccaccgg agagattgcc gacgatcttc
     3841 tgctggtcgc cgcccttgaa gttgaagcgc ccaaggtaag cgcgcgacgg catctcgaac
     3901 ctgccgacct gcaggatgtc gaggccgccg gagatcgctt cccagaccga cttgtcattt
     3961 ggcagcgatt cgcgtgtttg ttcaaccgcc gcgatctgta cgctcggtcc catgacgatc
     4021 tcgccggcat ctggcttctc ctgcccggtg atcatgtgga acagcgtcga cttgccggcg
     4081 ccgttggggc cgatgacgcc gacgatcgcg ccgggaggca ctgcaaagct gagactgtcg
     4141 atcagcaagc ggtcaccata acccttgctg acgttcttga attcgatcac ctggttgccg
     4201 agccgctccg ccaccgggat gaagatttcc tgcgtttcgt tgcgggtctg gtactcgtag
     4261 gacgacaact cgtcgaagcg cttcaggcgt gccttgctct tggcctgacg ccccttggcg
     4321 ttctggcgca cccactccag ctcgttcttc atcgtcttga tgcgcgccac ctcggacttg
     4381 gcttcacgct cgaggcgctc ttccttctgt tccagccacg agctgtagtt gcccttccac
     4441 gggatgccca tgccgcggtc gagttcgaga atccactcgg cagcgttgtc gaggaagtag
     4501 cgatcgtgcg tcaccgccac gacggtgccc gggaagcgcg agaggaactg ctcgagccag
     4561 tcgacacttt cggcatcgag gtggttggtc ggctcgtcga gcagcagcat gtcgggcttc
     4621 gacagcaaga gcttgcacag cgccacgcga cgtttctcac cgccggaaag cttggcgatc
     4681 gacgcgtccc acggcggcag gcgcagcgca tcggccgcaa tttccatctg gtgctcgacg
     4741 tcggcgcccg aggtcgcgat gatcgcctcg tacttggctt ggtccgccga cagcttgtcg
     4801 aaatccgcat cgggctcggc atacgccgcg tagatctcct cgagcttctg cttggcctgc
     4861 atcacttcac ccaggccggc ctcgacttcg tccttgacgg tcttggccgg gtcgagctgc
     4921 ggttcctgcg gcaggaagcc gatcgaaagc cccggcatcg gcaccgcttc gccctcgatg
     4981 tccgtgtcga gcccggccat gatcctcagc aacgtcgatt tgccggaacc gttgagcccg
     5041 agcacgccga tcttggcgcc ggggaagaag ctgagggaga tgttcttgag gatctcgcgc
     5101 ttgggcggaa cgatcttgcc cacgcggatc atggtgtaga cgtattgagc catggccgat
     5161 gcaaaaggaa gcgcgaaaag tcgcgcagat taccggatcg gccgcgtcac ttgacgggcc
     5221 gcggcaggag atggccgggc atgcgcaagg ccccggtgcg gaaggcgttg ctcgcgccct
     5281 ggtaatagcc gatcgcttcg ttggtgaagt gccgggcttc gtcgctcggc ggcagctcgc
     5341 ggccactcgc ggcatcgata atgcgcacgc gccgctgcgg ccgcagctcg accagcatgc
     5401 cgtgctcgta gtaaccgacc gtctggtagt tggccatcat tgcgcgctgg tgcgtgcggc
     5461 cttcattcaa tatgtcctgc ccgaagaaac gcgagacgta attgaagttc agcagcgcga
     5521 gcacggtcgg ggcgacgtct atttgagaag cgatggtgtc gatgcgcgcc ggagcgatgt
     5581 gcttcggggc gtagatcagc agcgggatgt ggaaacggtc gagcggcagg tcggttttgc
     5641 cacgcgacgc cgcgcaatgg tcggcaacga tgacgaacac cgtatcgtcg aaccaggcat
     5701 gcttgcgcgc gcgctcgatg aagtcaccga tcgcccaatc ggtgtatttc acggcaccgt
     5761 cggcaccact cttgggagca atgtcgatgc gtcccgccgg atagaaatag ggccggtggt
     5821 tcgacgtcgt catcacgtgc gcaaagaact tcttgccggc aatgtggcgc ttatccagtt
     5881 cacgcaccgt cagcgagaag agatcctcgt cagccacgcc ccagatgttc tcgtaatgaa
     5941 tgtcggcctt gtcgagcgca gtgcggtcga tgacggtgta gccgttgccg ccgaagaagt
     6001 gctccatgtt gtcgaagtag caatagccgc catagacgta gagcggctcg tagcccttct
     6061 cggcaaagac ctcgcctacg gtgaacaggt gatcgttgtt gggacgcttg acgactgaat
     6121 gccccggcgt cggaggcagt gacagggcca gggcttcgag cccgcgcact gtccgcgtgc
     6181 ccgtcgcata gagccgcgtg aagagcagtc cctcaagcgc gaggcgatca agctgcggcg
     6241 tcaggccttt cgcgttgccg aacgcgccca tgtagtcggc ggagagcgat tcaaccgaga
     6301 tcagcacgac gttgagcggc ttctccggcg cggcgcctcg gatcacgcgc tcgatcggca
     6361 gtccggcgtc gccggtgaac tcgaccggcc ccgcgtcggt gaattcttca cgtagttcct
     6421 tgtaagcctg ctcgatcggc accgtcgcat agaagcggtc gtagtcgatc tcgttggaat
     6481 tgaacgcgtg gaaaaactcc cagtgaccgt tgcccgccaa ctgctgcgac tggacgctgc
     6541 tcgagaactc tttccaagtc gacggaacca gcaggtagga cgccagcgcc accgcgatgt
     6601 aaccggccat ttgcagggat cgccggccga aggcgggacc tggcgcctgt gcagtccgcc
     6661 agatcggccg cgttgccagc gcgacaacga cggccgtcgc ccctgccagc cccagcagga
     6721 tcggagtcat ccggtacgac tcgcggatgt tgccgatcac ctcgcgcgtg tagatcaggt
     6781 aatcaaccgc gatgaagttg aagcgcgaag aaaattcgtt ccagaacacg aattccgata
     6841 cggccgtgaa cacgaagatg ccgaatgcaa tcgtggccag tacgatcgcc gcaataccga
     6901 aagcccgacg cgcacggtcc gcgggccaca gccacgcgag cagcgcgatc ggcagcagcc
     6961 agaacgtggc ggccgccagg tcgtaggtca gcccgatcaa cagccacccg gcaactcgcc
     7021 agggcatgaa ctcggccgac tcgttgttga agattgccag tccgacacgc gtgagcagac
     7081 tcaccgcgag ataggcgaca acgacgatgc cggcgacggc gaagcgtccg gggaaaagcc
     7141 gcttgaacgc gcgaaccagg atcgcgcgcc agggcgtaag gagtgtcatc gagataagcg
     7201 agaagacccg agttcgggga ccgcggctag cggtcactcg ctaagaggcg agttgttgaa
     7261 gtgtacgctt gcgtccgctc tgccgattgc attgggcaag agcgagcact ccacttctga
     7321 ctgctgactt gaccgctttt ctcatctcca tcggcgtcgt tgccctcgcc gagatcggcg
     7381 acaagacgca gttgcttgcg cttgcactgg ccgcgaagta ccgcaagccg tggccgatca
     7441 tctttggcat
//
LOCUS       DATIRT010000329         1743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1526209, whole genome shotgun sequence.
ACCESSION   DATIRT010000329 DATIRT010000000
VERSION     DATIRT010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526209"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..835)
                     /locus_tag="VLN25_07515"
     CDS             complement(77..835)
                     /locus_tag="VLN25_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HSQ10448.1"
                     /translation="MKKSIKLGLLASALMAITGTVSAQMKDVTVVDTKESPYVIDARG
                     VIVRDPFGLCWRTGSWSVDRAAKTVIKDAPFPNWTAGCECDKDLMVKPVCEPPPPPPP
                     PPAPTPPPPPPAPPAPTSEKVTFAADALFDFDKAVLRADGKAKLDDLVSKLAGVSLEV
                     IIAVGYTDRIGSLAYNQKLSVARAQAVKDYLVSKGIEPNRVYTEGKGPANPVKQCPDP
                     SAKGEIRNRAQLIQCLQPNRRVEVEVVGTRPVRR"
     gene            complement(930..>1743)
                     /locus_tag="VLN25_07520"
     CDS             complement(930..>1743)
                     /locus_tag="VLN25_07520"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSQ10449.1"
                     /translation="DDALVSFTLAPHAPYTVEDATLTKIAVLAEELDLPVHIHLHETV
                     DEVAESVRRYGQRPLARLDRLGLVTERLIAVHAVHLDAAEIALLAQRGASVAHCPASN
                     LKLGSGIAPVTEILAGGINVGLGTDGAASNDRLDMVSEMRLAALLAKGASRDPSVLPA
                     PAVLEAATLGGARALGLERRIGSIEIGKEADLAAFDLSEIETQPVFNPLSQLVYSAGR
                     EHATDVWVAGRSVVRTRQVLAEAQGAASGSPAATLLAWQNRCRRVLQTAGLA"
BASE COUNT          305 a          562 c          564 g          312 t
ORIGIN      
        1 ttcgaatgtt actggcccaa acgccagggc aaaaaaaagc cccgccgaag cggggctttg
       61 ctagcgaaaa gatcgcttag cgacggaccg ggcgggtacc gaccacctcg acctcgacgc
      121 gacgattcgg ctgcaggcac tggatcagct gcgcgcggtt gcgaatctcg cccttggcgg
      181 atggatccgg gcactgcttg accgggttcg ccgggccctt gccttccgtg tagacacggt
      241 tcggttcgat gcccttgctc accaggtagt ccttcacggc ctgagcacgc gcgaccgaca
      301 gcttctggtt gtacgccaga gaaccaatgc ggtcggtgta accgactgca atgatgacct
      361 cgaggcttac gccagccagt ttcgacacga gatcgtcgag cttggccttg ccgtcagcac
      421 gcagtaccgc cttgtcgaaa tcgaacaggg cgtctgccgc aaacgtcacc ttctcgctcg
      481 tcggagcggg cggtgccggg ggcggcggtg gaggcgtcgg agccggcggt ggcgggggcg
      541 gcggaggcgg ttcgcagacc ggcttgacca tcaggtcctt gtcgcactcg cagcctgccg
      601 tccagttggg gaacggcgca tccttaatta ccgttttcgc ggcgcggtcg acgctccacg
      661 aacccgtacg ccagcacagg ccgaacggat cgcggacgat gacgccacgc gcgtcgatta
      721 catacgggct ttccttggta tcaacaacag tgacgtcctt catctgggcc gaaacggtcc
      781 cagtgatggc catcagtgcc gatgccagga ggcccagctt gatggatttc ttcatataac
      841 tcctcactcg agattgcgga gaccggccgg ggtaccaact cggccggata tggccggcac
      901 tgcatcggcc gatgatggcg caagaacctt cacgccaaac ccgccgtctg cagaacccgc
      961 cgacagcgat tctgccacgc gagcagggtc gcggccgggg accccgacgc cgctccttgc
     1021 gcttcggcaa gcacctgtct cgttctgaca acagaccggc cggccaccca gacatcggtc
     1081 gcatgctcgc gaccggcgga atagaccagc tgtgagaggg gattgaagac cggctgggtc
     1141 tcgatttcgg atagatcaaa ggcggcgaga tccgcctcct tgccgatctc gatcgagccg
     1201 atgcggcgct ccagccccag tgcccgagcc cctccgagcg tggcggcttc caggaccgcc
     1261 ggcgcgggca gcacgctcgg gtcgcgactt gcccccttgg ctagcagggc ggccaggcgc
     1321 atttccgaca ccatgtcgag tcggtcattg ctggcggcgc cgtcggtgcc caggccgaca
     1381 ttgattcccc cggcgagaat ctccgttacc ggcgcgatac cgctgccgag cttgaggttc
     1441 gatgccgggc aatgcgccac tgaggcacct cgttgcgcaa gcagagcgat ctcggccgcg
     1501 tcgaggtgga ccgcgtggac ggcgatcagt cgttctgtca ccagtccgag ccggtcgagt
     1561 cgtgcgagcg ggcgttggcc ataccggcgt actgattcag cgacctcatc aaccgtttcg
     1621 tgcagatgga tgtgcaccgg cagatcgagc tcttcggcga gtacggcgat ctttgtcagc
     1681 gtggcgtcct cgaccgtgta cggggcatgc ggtgcgagcg tgaagctcac cagcgcgtcg
     1741 tcg
//
LOCUS       DATIRT010000330        13852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1624691, whole genome shotgun sequence.
ACCESSION   DATIRT010000330 DATIRT010000000
VERSION     DATIRT010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13852
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624691"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3080)
                     /locus_tag="VLN25_07525"
     CDS             complement(<1..3080)
                     /locus_tag="VLN25_07525"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSQ10450.1"
                     /translation="MKDAQPTAGMVSSPPTVDMPLSAPKLAWVRWSVGALALILLVTL
                     AVPLLLSQQFKLDHPVTLQSFEWAHRIIAGSLGVVLFAWIVNAQRAQHRARVRARLWE
                     QSIESMKVGVALWDAGDRLIGCNDAYRALYSEVADQLGAGRTYRELMTAYFEVAPAGV
                     VDGRTLDEFISDGERRRRRGSEVSEVVRHHRGRWLLMTDCRTANGGIICFRSDVTEQK
                     VIEHELTKRRKLIDDLADLTYDWFWRQDANGHFVEFSSAMEDHVKAPPDRLLGQRRED
                     MPGFEADPNQYAEYRARLAQRQPFPWFTYRARRGDGTPMWIAVTGKPIFDEKGEFQGY
                     YGAGRDVTEREDTVVALRRSEERFRALTMLATEWYWETDTDLRVTSVRGEPARQERVA
                     EWTLNRRFWEFDHIDPAFAVDWTVLRERVARRESFRNVKLKLRNGEDVSYYELAGQAV
                     VERGEYVGYRGLAWDVTERESLISRISDSEARFRALTELSSDWYWEMDENLRFTLLRQ
                     GARGSFGLNDEEVIGKHRWELPGELIQPSSWDEHRATLLGRRPFRDVMFRRWMPDGSL
                     VYHLTSGDPVFDAEGSFRGYRGIGKNISDQIRAQERIERLATVDALTQLSNRQTFDER
                     AGRLLATSYAEGKRCSLLFIDLDNFRLLNNGYGHRVGDEMLAIVASRMRDVVAEPHLL
                     GRRGGDELVVLLVDLQRPDAAVEVARKLIDSICEPARILGMEIAVTASVGISFFPQDG
                     IDLDSLLNAADAAMYQAKDSGRRTYAFYTQTVARRVDLRLRLEQRLRKAVESRDFKLF
                     YQPLVSLADGKMVGAEALIRWRDAELGDISPAEFIPIAEESGLIVGLGDWVLREACRA
                     RQEWRHLQLDIPPVSINMSGVQLRQLGCVEGLLDILNAYEVSPSEIEVEVTETGLLDT
                     SAVSRENLVRMRNAGVRLALDDFGVGFSSLAHLRDLPIHRLKIDRSFTVECMRDARTL
                     TIVKAVIEMARSIGISVTAEGIETQAQQTWMQHLGCDAAQGYLFARPMPGDDFI"
     gene            complement(3244..5232)
                     /locus_tag="VLN25_07530"
     CDS             complement(3244..5232)
                     /locus_tag="VLN25_07530"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019254672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HSQ10451.1"
                     /translation="MQSKPRTDQRKAADYGEASIRVLKGLEPVRQRPGMYTRTDSPLH
                     IIQEVIDNSSDEMLAGFGHRIDVTLHADGSVSVDDDGRGIPVGLHPEEKVPVVEIVFT
                     RLHAGGKFDKRAGGAYSFAGGLHGVGVSVTNALSSRLDVTVWRDGKLHTIGFADGELS
                     QPIKSVSAKGQAKSSGTRVRAWPNAKYFDAAIVPVDELERLLRSKAVLLPGTEVSLTL
                     EKTGATQRWRYEGGLREYLLGSLPADPMLPPFESSGFASADDETYAEGEGASWVVVWT
                     EDGAITRESYVNLIPTPAGGTHEAGLRDGLFNAVRAFVDAHGLLPKGVKLLPEDVFAR
                     ASFVLSAKVLDPQFQGQIKERLNSRDALRLVSSCIRPQLEIWLNQHPNEGRQLAELVI
                     RQAQARARAGQKVEKKKGSAVAVLPGKLTDCESTELTRNELFLVEGDSAGGSAKMGRD
                     KEFQAILPLRGKVLNTWEVERDRLFGNAEVHDIAVAIGVDPHGPSEAVNLEGLRYGKI
                     CILADADVDGSHIQVLLLTLFFRHFPQLVDRGHIFVARPPLFRVDVSAQGKRPPRRLY
                     CLDDAERDATLEKLADEGVRETAWSIARFKGLGEMSPEQLWETTMAPDTRRLLRVGLG
                     ELTADGTAAMMTMLMGRAESQARRAWLEEHGHEVEADV"
     gene            5466..5537
                     /locus_tag="VLN25_07535"
     tRNA            5466..5537
                     /locus_tag="VLN25_07535"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5499..5501,aa:Arg,seq:cct)
     gene            5765..6880
                     /locus_tag="VLN25_07540"
     CDS             5765..6880
                     /locus_tag="VLN25_07540"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HSQ10452.1"
                     /translation="MPSIKTNFDNIFRFGAAAIILVGIFMVLLPFLPALVFAGVLAIA
                     TWPLVERLRRAVGGNATVTATVMLVAILVLVIIPFTLLSAAAADQIPGLVTLVKGWLQ
                     DGVHAPEQLKAIPLIGERVYAQLESLLIDREQLSALLQRVLEPAGRLSLAFVRVLGDG
                     ILQLLLVTFIVFFYYRDGDRIVALLRRVSDRLSGSMSSEMLTIVTGTTRSVFVGIVGT
                     AAAQALVALIGFLIAGVPGAFMLSVATFILSVVPMGPVLIWGSAAIWLYVKGETGWAI
                     FMVIWGAVVISSIDNFVKPILIARGSTLSLGMIFLGVLGGVIAFGFIGVILGPVLLAL
                     GFALGRAWVSAQDARTTVTKEHAVTDAQPAAAPLRKD"
     gene            complement(6877..7470)
                     /gene="orn"
                     /locus_tag="VLN25_07545"
     CDS             complement(6877..7470)
                     /gene="orn"
                     /locus_tag="VLN25_07545"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HSQ10453.1"
                     /translation="MSQPPLESAVRAEHYGEMNFVWVDMEMSGLNPDSDRVLEIAVVV
                     TDAELEVLAEGPVLVIAQPERVLLGMDSWNTATHGRSGLTAKVRSSTLDESTASDILI
                     EFLQRHVPRGKSPMCGNSICQDRRFMARSMPRLEAYFHYRNLDVSTLKELARRWNPTV
                     YRSFEKKSRHEALADIYESIDELKHYRAQWLRTATAS"
     gene            7608..8870
                     /locus_tag="VLN25_07550"
     CDS             7608..8870
                     /locus_tag="VLN25_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HSQ10454.1"
                     /translation="MSQLLSLTFVVFLLLMVGLKYWLSWRQVRHVGRHADAVPSQFAD
                     RVTLEAHRKAASYTIAKTRLALIETAVGAALLVLLTLLGGLQWIAGVLRNHIDEGFAF
                     QLFFVVVVVVTLGIVDLPFSWFRQFHIEQRYGFNRMTPALWLTDLAKSFALAAVLGLP
                     LLAAVVALMRGAGEWWWIYAWMLWVSFNSLVLVIYPTLIAPIFNKFEPLKDSGLAQRI
                     AELLSRTGFSSKGVFVMDGSRRSAHGNAYFTGLGRAKRIVFFDTLVDRLQPSEIEAVL
                     AHELGQFKLKHIAKRLAFSFIASLIFLALLGWLAARPWFYTGLGVTPALEGGNEGAAL
                     VLFMLVLPVFTFVFAPVASHFSRRHEFEADAFAARHASAQDLVNALVKLYEDNASTLT
                     PDPIHSAFYDSHPPASLRVARLAAVAHA"
     gene            8867..9775
                     /gene="rsgA"
                     /locus_tag="VLN25_07555"
     CDS             8867..9775
                     /gene="rsgA"
                     /locus_tag="VLN25_07555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HSQ10455.1"
                     /translation="MKTAGRSDLPRFPARVIATFGRTSLLEYDAGASWSAVRRGRRSD
                     LVVGDRVVASASAPGQAAVESIEPRTSLLYRADAFRTKELAANVDQVIIVFASRPTFN
                     LWFLWKALVAARTADIEGIVVRNKTDLEDGAEAAQAALGQIGRLGWRTAAVSARSGPD
                     ETLATLRQITQGLSSLLVGQSGMGKSTLLNLLVPDAQARTREYSERFDLGRQTTTAAR
                     WFRLPYGGALVDTPGFQEFGLAHLSSAQIEAGFPEFESHLGKCRFADCRHLVEPDCAI
                     RTAVGAGDVVSGRYDFYRSLIDGVTR"
     gene            9772..10080
                     /locus_tag="VLN25_07560"
     CDS             9772..10080
                     /locus_tag="VLN25_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HSQ10456.1"
                     /translation="MKLLARADHLVLATIWAESLRAAGIRCQLRNTTLSGAIGEIPFL
                     ECAPQVWIERDADESRAQEILQELRRPVRGPNWHCAACGESSEPQFGACWQCGAARPA
                     "
     gene            complement(10130..11095)
                     /locus_tag="VLN25_07565"
     CDS             complement(10130..11095)
                     /locus_tag="VLN25_07565"
                     /inference="COORDINATES: protein motif:HMM:NF005792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HSQ10457.1"
                     /translation="MAWLSLVITLLIEQLRALPSENPVYASVRQVSEWATHNLNAGRP
                     RHGFYAWLLVVVGLTIAVGALYSVLVSINWFLGLALNVLVLYFALGFRQFSHRGTEIQ
                     IALANGDLAAARHELTAWKRLSDPTFSAADLASEEVVRQAIEHGLLLSQRHVFGVYFW
                     FVLLPGPMGAVFYRLAEHSARVWNRAPLPPTPPDRFGGFARQAFAWIDWAPARLTALG
                     YAIVGDFEGAIYCWRQVARKPGADGMPSPDSRTLILATAGGALGLRLMSEAESARYFD
                     ESGNEGAGLAEPRPPAVRAVVGLVWRSLLLWLSLLLLMTLAHWLA"
     gene            complement(11138..11848)
                     /locus_tag="VLN25_07570"
     CDS             complement(11138..11848)
                     /locus_tag="VLN25_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007748311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HSQ10458.1"
                     /translation="MSSPGATPRRPLFDPETVPHDPAFDNPLARGPALAAERLRPSAL
                     RQRFATPIAWQPELRADPRLFLPDMEPRPAAVLVPLIVRGNDVQVLLTQRTAHLNDHA
                     GQISFPGGSVEEHDTDPIATALREAEEEVGLRSAIVDIVATLPEYTTATGFRVTPVIG
                     MVEQPFTLTLDTFEVSETFEVPLAFLMDPAHHERRIVALDDFARTFYAMPYEAMRRYF
                     IWGATAAMLRNLYQFLRA"
     gene            complement(11928..12362)
                     /gene="rplS"
                     /locus_tag="VLN25_07575"
     CDS             complement(11928..12362)
                     /gene="rplS"
                     /locus_tag="VLN25_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HSQ10459.1"
                     /translation="MNIIQKLEQEELARVSVNKSIPDFAPGDTVIVNVNVVEGTRKRV
                     QAYEGVVIGRRNRGLNSSFVVRKVSSGEGVERTFQLYSPTIASIEVKRRGDVRRAKLY
                     YLRDRSGKSARIREKLTHRSAADKAAADKAAAVDAAADEAAE"
     gene            complement(12431..13198)
                     /gene="trmD"
                     /locus_tag="VLN25_07580"
     CDS             complement(12431..13198)
                     /gene="trmD"
                     /locus_tag="VLN25_07580"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008864759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HSQ10460.1"
                     /translation="MRFDVVTLFPAMFAAITEHGITRRAFELGLWSLATWNPRDFTSD
                     VHRTVDDRPYGGGPGMVMMAEPLALALDAVRQEGGAARVVALAPNGHPLTDARVRQWA
                     AQGGSLTVVCGRYEGIDQRFLDVCVDEVVSVGDFVMSGGELAAMALIDAVVRQLPGAL
                     KDESVDDESFATGLLDGPHYTRPESWRGTAVPAVLMSGHHADIEIWRRREALRATMRD
                     RPDLIRAARAEGRLTARDERDLRELAQMGAAESESAA"
     gene            complement(13211..13750)
                     /gene="rimM"
                     /locus_tag="VLN25_07585"
     CDS             complement(13211..13750)
                     /gene="rimM"
                     /locus_tag="VLN25_07585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HSQ10461.1"
                     /translation="MNVEGSAAATPPPDLVELGAVHSAYGVKGWVRVALRGSDGGVLQ
                     NAGTWWLLEPGGVRPIVPQSCRRHGAALLAKWAGCESKEMAEALKGVALAVPRSEFPA
                     PAEGEYYWADLMGCRVVNRSGDHLGDVAGLRENPGGQWLEVSDGPDGNVRLIPLVEQF
                     VESVDLPQRIVRVDWNNEW"
     gene            complement(13750..>13852)
                     /gene="rpsP"
                     /locus_tag="VLN25_07590"
     CDS             complement(13750..>13852)
                     /gene="rpsP"
                     /locus_tag="VLN25_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900528.1"
                     /note="binds to lower part of 30S body where it stabilizes
                     two domains; required for efficient assembly of 30S; in
                     Escherichia coli this protein has nuclease activity;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HSQ10462.1"
                     /translation="DRLSYWAGVGARVSPTVARLVKQHAPKQGAAAA"
BASE COUNT         2345 a         4648 c         4359 g         2500 t
ORIGIN      
        1 ttgatgaagt catcgcccgg catcgggcgt gcgaaaagat agccctgcgc cgcgtcgcaa
       61 cccaggtgct gcatccatgt ctgctgcgcc tgggtttcga tgccctcggc tgtgacgctg
      121 ataccgatcg agcgcgccat ctcgatcacc gccttcacga tcgtcagcgt acgcgcgtcg
      181 cgcatgcact cgaccgtgaa gctgcggtcg atcttcaggc ggtggatcgg caggtcgcgc
      241 aggtgcgcca ggctggaaaa gccgacgccg aagtcgtcga gcgcgagccg cacgccagcg
      301 ttgcgcatgc gcaccaggtt ctcgcgcgac accgccgagg tatcgagcag gccggtctcg
      361 gtgacttcga cctcgatctc cgacggcgac acttcatacg cgttgagtat atcgagcagt
      421 ccctcgacgc agccgagttg acgcaactgc acgccggaca tgttgatcga caccggcggg
      481 atgtcgagct gcagatgacg ccattcctga cgcgcccgac aggcctcgcg cagcacccag
      541 tcgccgagcc cgacgatgag ccccgattcc tcggcgatcg ggatgaactc ggccggcgag
      601 atgtcgccaa gctcggcgtc acgccagcgg atcagggctt cggcgccgac catcttgccg
      661 tccgccagcg aaaccagcgg ctgatagaac aacttgaaat cgcgcgactc gaccgccttg
      721 cgcaggcgct gctcgagccg cagacgcagg tcgacgcgcc gcgccacggt ctgcgtatag
      781 aaggcatagg tgcggcggcc gctgtccttg gcctggtaca tcgccgcatc ggccgcgttc
      841 agcagtgaat cgaggtcgat gccgtcctgg ggaaagaagc tgatgccgac cgaggcggtg
      901 acggcgattt ccatgccgag aatgcgcgcg ggctcgcaga tcgaatcgat cagcttgcgc
      961 gcgacctcga cggctgcatc gggccgctga agatcgacca gcagcacaac cagctcatcg
     1021 ccaccgcggc ggccgagcag gtgcggctcg gcaacgacgt cacgcatgcg gctggccacg
     1081 atcgcaagca tctcgtcgcc gacacggtgg ccgtagccat tgttcagcag gcggaagttg
     1141 tcgaggtcaa tgaacagcag cgaacagcgc ttgccctcgg catacgacgt tgccagcagc
     1201 cgtcccgctc gctcgtcgaa ggtctgccgg ttggaaagct gcgtcagcgc gtctaccgtg
     1261 gccagacgct cgatgcgttc ctgtgcgcgg atctggtcgc tgatgttctt gccgatgccg
     1321 cgatagccgc gaaagctgcc ctcggcatcg aaaacgggat cgccgctggt caggtggtag
     1381 accagcgagc cgtccggcat ccagcggcga aacatcacgt cgcggaacgg ccggcgtccg
     1441 agcagggtcg cacgatgctc gtcccatgac gacggctgga tcagttcccc gggcagctcc
     1501 cagcggtgct tgccgatcac ctcttcgtcg ttgagaccga atgaaccgcg cgcaccttgc
     1561 cgcagcagtg tgaagcgcag gttctcgtcc atttcccagt accagtccga cgatagctcg
     1621 gtgagtgcac ggaagcgcgc ttcgctgtcg gagatccgcg aaatcagcga ttcgcgctcg
     1681 gtcacatccc aggcaaggcc ccggtagccg acgtactcgc cacgctccac caccgcctgg
     1741 ccggccagtt catagtagga aacatcctcg ccgttacgca gtttcagctt gacgttgcga
     1801 aacgattcgc gccgcgccac gcgctcgcgc aggacggtcc agtcgacggc aaacgcggga
     1861 tcgatgtgat cgaactccca gaaccgccgg ttcaatgtcc attcggccac gcgctcctgg
     1921 cgggcgggtt ccccgcgcac cgacgtgacg cgcagatcgg tgtcggtctc ccagtaccac
     1981 tcggtcgcga gcatcgtcag tgcccggaac cgctcctcac tgcgacgcag ggcgacgacc
     2041 gtgtcttcgc gctccgtgac gtcgcgcccg gccccgtagt agccctggaa ttcgcctttc
     2101 tcatcgaaga tcggcttgcc ggtgactgca atccacatgg gagtgccgtc accgcgccgt
     2161 gcccgatacg tgaaccaggg aaacggctgg cgctgcgcaa gccgcgcgcg gtactcggcg
     2221 tactggttcg gatcggcctc gaaacccggc atgtcctcgc gccgctgccc gagcaggcga
     2281 tcgggcggcg ccttgacgtg gtcttccatg gccgaagaga actcgacgaa gtggccgttg
     2341 gcgtcctggc gccagaacca gtcgtaggtc aggtcggcaa gatcatcgat cagtttgcgg
     2401 cgcttggtca gctcgtgctc gatgaccttc tgttccgtca catcgctgcg gaagcagatg
     2461 atgccgccgt tggccgtacg gcagtccgtc atcaggagcc accggccccg gtgatgacgc
     2521 acgacctcgc tgacttcgga gccccgacga cgacgacgtt ccccgtcgct gatgaattcg
     2581 tcgagggtcc gaccgtcgac gaccccggct ggcgccacct cgaagtaggc ggtcatcagc
     2641 tcacgatagg tccgcccggc cccgagctga tcggccactt ccgaatacag cgcccgatac
     2701 gcatcgttgc agccgatcag cctgtcaccg gcatcccaca gcgccacgcc gaccttcatc
     2761 gactcgatcg actgttccca gagcctggcg cggacccgcg cccggtgctg cgcccgctgg
     2821 gcattcacga tccaggcaaa caacaccacc ccgagcgagc ccgcaatgat ccggtgcgcc
     2881 cattcgaacg actgaagtgt gaccggatgg tcaagcttga actgctgcga aagcagcaac
     2941 gggactgcca gcgtgacaag gaggatcagc gccagcgcgc ccacggacca gcgcacccag
     3001 gccagtttcg gcgcggaaag tggcatgtcc acggtgggcg gagagctcac catgccggca
     3061 gtaggctgcg cgtccttcat gcggaagtga atggcccctg ttttgatcgc gccctctggt
     3121 cggcggtcgc gaccgcgcgc aagtatgccc gcaagcccgg gccttcaata caccccagta
     3181 acgggaggta gagggtcaat ccgcgccgcg tagcgccgcc acgttgtgct gccttgtcgc
     3241 aggctagacg tccgcttcga cctcgtggcc gtgctcctcg agccaggcgc gtcgcgcctg
     3301 gctttcggca cgccccatca gcatcgtcat catcgccgca gtcccgtcgg cggtaagctc
     3361 gccgagaccg acccgcagca gccgccgcgt gtccggcgcc atcgtcgtct cccacagctg
     3421 ttccgggctc atctcgccga gccccttgaa gcgcgcaatg ctccacgcgg tttcccggac
     3481 cccctcgtcg gccagcttct cgagcgtggc atcacgctcg gcatcgtcca ggcagtacag
     3541 acggcgcggc ggccgcttgc cctgtgcgct gacgtccacg cgaaacagcg gcggccgggc
     3601 gacgaagatg tgcccgcggt cgacgagctg cggaaagtgc cggaagaaca gcgtcagcag
     3661 cagcacctgg atgtgtgagc catcgacgtc ggcatccgca aggatgcaga tcttgccgta
     3721 gcgcaggccc tcgagattca ccgcttccga cgggccgtgc gggtcgacac cgatcgccac
     3781 tgcgatgtcg tgcacttccg cattgccgaa caggcggtcg cgttcgactt cccacgtatt
     3841 gagaaccttg ccacgaagcg gcaggatcgc ctggaattcc ttgtcgcgcc ccattttcgc
     3901 cgagccgccc gccgagtccc cctcgaccag gaagagttcg ttgcgcgtga gctccgtgga
     3961 ctcgcagtcg gtgagcttgc caggcagcac cgccaccgcc gagccctttt tcttctcgac
     4021 cttctggccc gcgcgcgccc gtgcctgtgc ctgccggatc acgagttcgg cgagctgtcg
     4081 gccttcgttc ggatgctgat tgagccagat ctcgagctgt ggccggatgc atgacgagac
     4141 aagccgcaac gcatcgcgac tgttcaggcg ttccttgatc tgcccctgaa actgcggatc
     4201 gagaaccttg gccgacagca cgaagctcgc ccgcgcaaag acgtcttccg gaagcagttt
     4261 cacgcccttg ggcagcaggc cgtgggcgtc gacgaacgcg cgcacggcat tgaacaaacc
     4321 atccctcagt ccggcctcgt gcgtgccacc ggccggcgtc ggaatcaggt tcacgtagga
     4381 ctcgcgcgtg atggcgccgt cttcggtcca gacgacaacc cagctggcgc cctcgccttc
     4441 cgcgtaggtt tcgtcatcgg ccgaggcgaa tccgctggac tcgaagggcg gcagcatggg
     4501 gtcggccggc aacgaaccga gcaggtactc gcgcagtccc ccttcatagc gccagcgctg
     4561 ggtcgccccc gtcttttcaa gcgtgagaga gacttcagtt cctggcagca gcacggcctt
     4621 gctgcgcaac aggcgctcga gttcgtccac cggaacaatc gcggcgtcga agtacttcgc
     4681 gttcggccac gcccgcacgc gcgtaccgct ggacttggcc tggcctttcg cggacaccga
     4741 cttgatcggc tggctcaatt cgccgtcggc gaagccgatc gtatgcagct tgccgtcgcg
     4801 ccagacagtg acgtcgagcc gggacgacag cgcgttggtc accgatacac cgacgccgtg
     4861 caagccaccc gcaaaggagt aggcccctcc ggcccgtttg tcgaacttgc caccggcgtg
     4921 aaggcgggtg aacacgatct cgaccaccgg taccttttcc tccggatgca ggccgaccgg
     4981 gatgccccgt ccatcgtcat cgacactgac cgagccgtcg gcatgcagcg tgacatcgat
     5041 acggtgtccg aatccggcga gcatttcgtc cgacgagttg tcgatcacct cctggataat
     5101 gtgcaacgga ctgtcggtgc gcgtgtacat gccgggacgc tggcgcaccg gctccagccc
     5161 cttgaggacg cgtatcgaag cctcgccgta gtcagccgct ttgcgttggt cagtacgtgg
     5221 cttgctctgc attgcaagaa acgggtgagg agttgtcccc agctgctgtg gaaaacatca
     5281 tcggaaatgc ccgtcaaggc gcgccattgc tggcaggcgt cgagttgatc acgaaatagg
     5341 caatcgccgc gactccgatc atgagcgcaa gcttgaaccg atcgcgatgt gtcgcgtgtc
     5401 ggaacccgtg cgaaccttcg tgtggcggcg gattctacgt gagggcgatc gctatactgg
     5461 ccgcactccc cgtagttcaa cggatagaac aagtgcctcc taagcgctag atctgggttc
     5521 aattcccggc ggggggacac ccgcgtagac gtttctcgcc tgcgcgagaa acgcccgcgg
     5581 gtgcgtcatc gcgcctgccg gcgcgatgaa cgcttccaga ccgccgcgtt gagtctcctg
     5641 ggtgcgaaaa atccgcgagc gtgtctcggc gccaagcgtc gagaccgttt ccagaggctt
     5701 cacagccccc agccccgtct tccgaacccg ctaagctgcg cccgtcctgc ccgggtcgaa
     5761 ccacatgccc tccatcaaga ccaacttcga caacatcttt cgcttcggcg ctgccgcgat
     5821 catcctggtc ggcatcttca tggtgctgct gccgtttctg ccggcgctgg tgttcgcagg
     5881 ggtgctggcg atcgcgacct ggccgctggt tgagcggctg cgacgcgcag tgggcggtaa
     5941 tgcgacggtg actgccaccg tgatgctcgt ggcaatcttg gtgctggtca tcattccgtt
     6001 caccctgctg tcggcggcgg ccgctgacca gattccaggg ctggtcacgc tggtcaaagg
     6061 ctggctccag gatggcgtcc atgccccaga gcagttgaag gcaatcccgc tgattggcga
     6121 gcgcgtctat gcccagctcg aaagcctgct gatcgaccgt gagcagctct cggcgctctt
     6181 gcagcgtgtg ctcgagccgg ccggccgcct ttctctcgcc ttcgtgcgcg tgctgggcga
     6241 cggcatcctg cagttgctgc tcgtgacgtt catcgtgttc ttttactacc gtgatggcga
     6301 tcgcatcgtt gccctgctca ggcgcgtgtc tgatcggctt tcgggcagca tgagctccga
     6361 aatgctgacg atcgtgacag gaacgacgcg cagcgtattc gtcggcatcg tcggcacggc
     6421 cgcggctcag gccctggttg cgttgatcgg gttcctgatc gccggcgtgc ctggagcctt
     6481 catgctgtcc gtggccacgt tcatcctgtc agtggtgccg atgggcccgg tgctgatctg
     6541 gggttccgcc gccatctggc tttacgtcaa gggtgaaacc ggctgggcca tattcatggt
     6601 gatctggggc gctgtggtga tcagcagcat cgacaacttt gtcaagccga tcctgatcgc
     6661 gcgcggctcg accctgtcgc ttgggatgat cttcctcggc gtgctgggag gcgtcatcgc
     6721 cttcggattc atcggagtga tactcggccc ggtcctgctg gcgttgggct tcgctctcgg
     6781 tcgcgcctgg gtcagtgccc aggacgcccg cacaaccgtc acgaaagaac acgccgtcac
     6841 tgacgcgcaa cccgcggcgg ccccgttgcg caaagactag gacgcggtcg cggtgcgcag
     6901 ccactgcgca cgatagtgct tgagttcgtc gatcgactcg taaatgtcgg ccagcgcctc
     6961 gtgccggctc ttcttctcga acgagcggta gaccgtcgga ttccagcggc gcgcaagctc
     7021 cttcagcgtg ctgacatcga ggttgcggta atggaagtac gcctccagcc gcggcatcga
     7081 gcgtgccatg aagcgccggt cctggcagat cgagttgccg cacatcggcg acttgccgcg
     7141 cggtacatgg cgctgcagga actcgataag gatgtcgctt gccgtggact cgtctagcgt
     7201 cgacgaacga actttggcgg tcagtcccga gcggccgtga gttgcggtgt tccagctgtc
     7261 catgcccagc aggacccgtt ccggctgagc aatgacgagc acggggcctt cggcaagaac
     7321 ctcaagttcg gcatcggtga cgaccacggc aatctcgagc acgcggtcgg agtccggatt
     7381 cagccctgac atttccatgt cgacccagac gaaattcatc tcgccgtaat gctcggcacg
     7441 aacggctgat tcgagagggg gctgtgacat aattcccgcg ttgatcgatc aatgcgcatc
     7501 gattcgatcg aagggcgcgc gattgtcgca cggcgaaccg gcggacaggc gtgccgcgcc
     7561 gatcctggcg gatgcgcgat gcgcttcgag taccgcacct cgtgtccatg tcccagcttc
     7621 tgtcgttgac gttcgtcgtc tttctgctgc tgatggtcgg cctgaagtac tggctgtcat
     7681 ggcggcaggt ccgtcatgtc ggacgacatg cggatgccgt cccgagtcag ttcgccgacc
     7741 gggtcacgct cgaggctcat cgcaaggcgg cgtcctacac gatcgccaaa acgcggctcg
     7801 cgctgatcga gaccgccgtc ggcgcggcgc tgctcgtact gctcacgctc ctcggcggcc
     7861 tgcagtggat cgccggtgtg ctgcgtaacc acatcgacga agggttcgcc ttccagttgt
     7921 tcttcgtggt ggtcgtcgtg gtgaccctcg gcatcgtcga tctgccgttt tcgtggtttc
     7981 ggcagttcca catcgagcag cgctacggtt tcaatcggat gactccggcc ctctggctga
     8041 cggatctggc caagtccttc gcgctggccg ccgtgctcgg attgccgttg ctggctgccg
     8101 tggtcgccct gatgcgcggc gcgggcgagt ggtggtggat ctatgcgtgg atgctgtggg
     8161 tgagcttcaa ctcgctggtg ctcgtcatct acccgaccct gatcgcgccg atattcaaca
     8221 agttcgagcc gctgaaggat tccgggctcg cgcagcgcat cgccgagcta ctgtcacgca
     8281 ccggattttc cagcaagggt gtgttcgtga tggatgggtc gcgccgttcc gctcacggca
     8341 acgcctactt caccgggctc gggcgcgcca agcgcatcgt cttctttgac acactggtcg
     8401 atcggctgca gccgtcggag attgaggccg tgctggccca cgaactgggg caattcaagc
     8461 tcaagcacat cgccaagcga ttggcgttct catttattgc gagcctcatt tttctcgcac
     8521 tgctcggctg gcttgcagcc cgcccgtggt tctacaccgg actcggtgtg acacctgcgc
     8581 tcgagggcgg caacgaaggc gctgccttgg tgctcttcat gctggtacta ccggtgttca
     8641 cgttcgtctt cgcgcccgtc gccagccatt tttcgcgccg gcacgagttc gaagcggatg
     8701 ccttcgcggc ccgtcatgcg tcggcgcagg acctcgttaa cgcactcgtc aagctctacg
     8761 aggacaacgc ctcgaccttg acccccgatc cgatccactc ggccttctac gactcgcacc
     8821 cgcccgcctc gctgcgcgtc gcacgcctgg cggccgtcgc gcacgcatga aaaccgccgg
     8881 gcgcagtgac ctgccccggt ttcccgctcg agtcattgcc acctttggcc gcacctcgct
     8941 gctcgagtac gacgccggcg cgtcgtggtc cgccgtgcgt cggggccgcc gcagcgatct
     9001 tgtcgtcggc gatcgtgtcg ttgccagcgc gtcagctccc gggcaggccg cggtcgagtc
     9061 gatcgagccg cggacgagcc tgctgtaccg cgccgatgcg tttcgcacga aggaactcgc
     9121 ggcgaatgtc gaccaggtca tcatcgtgtt cgcctcgcga ccgactttca atttgtggtt
     9181 cctctggaag gcactggtgg cggcgcgcac tgcggacatc gagggcattg tggtgcgcaa
     9241 caagacggac ctcgaggacg gcgccgaggc cgcacaggcg gcgctggggc agattggccg
     9301 actgggctgg cgcactgccg ccgtttctgc gcgctccggg cccgacgaaa ccctcgcgac
     9361 gctccggcag attacgcagg gactcagctc tttgctggtc gggcaatcgg gcatgggcaa
     9421 gtcgacactg ctcaatctgc tggtaccgga cgcgcaggca cgaacacgtg agtattcgga
     9481 acgattcgat ctcggcagac agacgacgac cgcagcacgc tggttcaggc tgccctacgg
     9541 gggcgccctc gtggacacac cgggctttca ggagttcggc ctcgcgcact tgtcatcagc
     9601 acagatcgag gcgggctttc cggagttcga atcccacctg ggcaagtgcc gattcgccga
     9661 ctgccggcac ctggtcgagc cggactgcgc tatccgtacc gcagtggggg ccggagacgt
     9721 ggtttcgggc cgctacgatt tctatcgatc attgatcgac ggggtcacgc ggtgaaactg
     9781 ctggcgcgcg cggaccacct ggtgctggcc acgatctggg ccgagtcgct gcgggcggca
     9841 gggatccgct gccagttgcg caacaccacg ctttccggtg ccattggtga aattcctttc
     9901 ctcgagtgcg cgccacaggt gtggatcgaa cgagatgccg atgagtcgcg cgcacaggaa
     9961 atcctgcagg aactgcggcg tccggtacgt ggaccgaact ggcactgcgc cgcgtgcggc
    10021 gaatcgtccg agccgcagtt cggcgcgtgc tggcagtgcg gggcggcgcg gccggcttga
    10081 tccgatggga tcgggcgtgt gaacctgtac cggcggtcct gtcgttgcgt caggccagcc
    10141 agtgcgccag agtcatcaac agcagcaagg agagccagag cagcagcgag cgccagacca
    10201 gtccgacaac ggcgcgcact gcaggcgggc gcggctcggc caatccggca ccttcgttac
    10261 ccgattcatc aaagtagcgt gccgactccg cttcggacat caatcgcaga ccgagtgcgc
    10321 caccggcggt cgcgagtatc agcgtgcgtg aatcggggga tggcatgcca tcggcccccg
    10381 gcttgcgcgc aacctggcgc cagcagtaga tcgccccttc aaagtcgccg acgatggcat
    10441 agccgagcgc cgtcagtcgc gccggggccc agtcgatcca ggcaaaggct tgacgcgcga
    10501 atccaccgaa acgatcgggc ggcgtcggcg gcagcggtgc gcggttccag acgcgggccg
    10561 agtgttccgc gagacggtaa aagaccgctc ccatcgggcc cggcagcagc acgaaccaga
    10621 agtacacccc gaacacgtgg cgctgtgaca gcagcagccc gtgctcgatc gcctgccgga
    10681 ccacttcttc cgacgcaaga tcggcggcgc tgaacgtcgg gtcgctgagt cgcttccatg
    10741 cggtgagttc gtgtcgggcc gccgcgaggt caccattggc cagtgcaatc tggatctcgg
    10801 tgccacggtg gctgaactgc cggaacccga gtgcgaagta caggacgagc acgttgagtg
    10861 ccagtccgag aaaccagttg atcgagacca gcaccgagta cagcgcaccg acggcgatcg
    10921 tgaggccgac gacaaccagc agccaggcgt aaaagccatg ccgggggcgc ccggcgttga
    10981 ggttgtgcgt tgcccactcg gacacctgcc gcacggacgc atacaccggg ttctcgctcg
    11041 gcagcgcgcg caactgctcg atgagcagcg tgatcaccag cgatagccag gccacttgca
    11101 tcctcctcgt tgcattgtag tggcggcctc cgcccgatca ggcgcgcagg aactggtaaa
    11161 ggttgcgaag catcgcggcc gtcgcgcccc agatgaaata gcggcgcatc gcctcatacg
    11221 gcatcgcgta gaacgtacgc gcgaagtcgt ccagcgcgac gatgcgccgc tcgtgatgcg
    11281 cagggtccat caggaacgca agcggaacct cgaatgtctc ggacacttcg aacgtatcga
    11341 gtgtgagcgt gaacggttgc tcgaccatgc cgatcaccgg agtgacacgg aaacctgtcg
    11401 cggtcgtgta ctccgggaga gtcgcgacga tgtccacgat ggccgagcgc agtccgactt
    11461 cttcttcggc ttcacgcagc gcggttgcga tcggatccgt gtcgtgttcc tcgacgctgc
    11521 cgcccggaaa actgatctgt ccggcgtgat cgttgaggtg agcggtgcgc tgggtcagca
    11581 gcacctgcac gtcgttgccg cgcacgatca gcgggaccag gactgcggca gggcgcggct
    11641 ccatatccgg aaggaacagg cgcgggtcgg cgcgcagctc gggctgccat gcgatcggtg
    11701 tggcaaatcg ctggcgtagc gccgacggcc gcagacgttc cgctgccaac gctgggcctc
    11761 gcgccagtgg gttgtcgaat gccggatcgt gcggcacggt ttccggatcg aacagcggac
    11821 gtctcggggt ggctcccggt gaactcatcg aatgccccag catgacgcga cagccgaaga
    11881 caaatgcaga gggccgccag cggcggccct cgaggcggac gggaagatta ttccgccgcc
    11941 tcgtcggcag cggcatcgac cgcggccgcc ttgtcggcgg cggccttgtc tgccgcgctc
    12001 cggtgggtga gtttctcgcg gatacgggcc gacttgcccg accggtcgcg caggtagtac
    12061 agcttggcgc ggcggacatc gccgcggcgc ttcacctcga tcgatgcgat cgtaggcgaa
    12121 tacaactgga aagtgcgctc gacgccctcg ccgctcgaca ccttgcgcac cacgaaggac
    12181 gagttcagtc cgcggttgcg acggccgatc acgacgccct cgtaggcctg cacgcgcttg
    12241 cgggtacctt cgacaacgtt gacgttgacg atcaccgtgt cccccggcgc gaagtcggga
    12301 atcgacttgt tgacggacac gcgcgccagt tcttcctgtt ccagcttctg aatgatgttc
    12361 attttcagct cctcattcca tcatcgtgcg gctgtcatcg ttgtgtgccg cccgagagga
    12421 tggattcact tcacgcagcg gactcggact ccgctgcacc catctgcgca agttcgcgca
    12481 aatctctttc atcgcgcgcg gtcagtcgtc cttccgcgcg cgccgcccgg atcaaatccg
    12541 gccggtcccg catcgtcgcc cgcaatgcct cgcgacggcg ccagatctca atatcggcat
    12601 ggtgccccga catcagcacc gccggcaccg cggttccgcg ccagctctcg ggacgcgtgt
    12661 aatgcggccc gtcgagcagt cccgtcgcga acgactcatc gtccaccgat tcgtctttca
    12721 gcgcacccgg caactgccga accacggcat cgatcagcgc catggctgcc aactccccgc
    12781 cggacatcac gaagtcacca accgacacga cctcgtcgac gcagacatcg aggaagcgct
    12841 ggtcgatccc ctcgtagcgc ccgcacacca cagtcagcga tccgccctgc gccgcccact
    12901 gacgcacccg ggcatccgtc agtggatgtc catttggcgc cagcgccacg acccgcgccg
    12961 ccccgccttc ctgcctgact gcatcgaggg caagcgccag gggttcggcc atcatcacca
    13021 tccctggccc gccgccgtac gggcgatcat cgacggtgcg gtggacgtcc gacgtgaagt
    13081 cccgcgggtt ccacgtcgca agcgaccaca agcccagttc gaaggcgcgc cgcgtaatgc
    13141 cgtgctcggt gatggccgca aacatcgcgg ggaaaagcgt gacgacgtcg aagcgcatca
    13201 cccgtccctg tcaccactcg ttgttccagt cgacccgcac tattcgctgc ggcaggtcca
    13261 cgctctcaac aaactgctcg accagcggga tcagccgcac attgccatcc gggccatcgc
    13321 tcacttcaag ccactggccg cccgggttct ctcgcaggcc ggccacgtca cccaggtggt
    13381 caccgctccg attaaccaca cggcacccca tcaggtcggc ccagtagtac tcgccctcag
    13441 cgggggcagg aaattcgctg cggggaaccg ccagcgccac gcccttcagg gcctcggcca
    13501 tttccttcga ctcgcacccc gcccacttgg ccagtagagc agcgccatgc cgtctgcacg
    13561 attgcggcac gatcggccgc acgccaccgg gttcgagcaa ccaccacgtg ccggcgtttt
    13621 gcagtacccc tccatcggag ccccgcagcg ccacgcgaac ccaacccttc acaccgtatg
    13681 cgctatgcac cgcgccgagt tccaccagat ccggcggcgg ggttgcagcc gcggatcctt
    13741 ctacattcac tacgcggcgg ctgcgccttg cttcggcgca tgctgcttga ccaggcgcgc
    13801 gaccgtcggc gacacacggg cgcccacgcc ggcccagtag ctgagccggt cg
//
LOCUS       DATIRT010000331         3414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_346757, whole genome shotgun sequence.
ACCESSION   DATIRT010000331 DATIRT010000000
VERSION     DATIRT010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346757"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..903
                     /locus_tag="VLN25_07595"
     CDS             103..903
                     /locus_tag="VLN25_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018057744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HSQ10463.1"
                     /translation="MLDRQGRLRDLSGVVEHVTSEHLSDKGLAKLAKINPESLPLVAG
                     SPRLGVPYLGISKFIAIGLNYADHAAESGVPVPSEPILFSKATTCINGPSDDIVIPRS
                     STKTDWEVELGVVIGTQAQYVARDKALDHVAGYCVINDVSERNFQLERGSQWDRGKGC
                     DTFGPIGPYLVTRDEVPDPQNLDMWLDVNGQPMQRGNTRTMVFGVAHLVSYVSQFMTL
                     LPGDIITTGTPPGVGMGRKPPQYLKAGDVVTLGIAGLGEQRQRVRAHA"
     gene            890..1978
                     /locus_tag="VLN25_07600"
     CDS             890..1978
                     /locus_tag="VLN25_07600"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HSQ10464.1"
                     /translation="MRTRDRVAGAVSSPPAVRHVSASSLTAWAIACLTRVGVRASDAE
                     TVARSLVQTSLWGIDSHGIARLGHYMARIKAGSIAPIPALRIKETGPCTATIDGGHGL
                     GIVVCTAAMAHAIGLARSNGVGIVGIGESSHCGAIGLYTRQATAAGLIGFAFTHADSI
                     VAPAGGAQKLLGTNPLSIAFPNPDGPPICLDMATSSIAWNRVMNARREGHALPEAVAI
                     NQSGTPTRDAQEAAALLPLGGSEYGHKGYALALMIDLLCGPLNGMPFADRIPPMFADL
                     QARRHLGSLVMALDPMRFAGGPTLALTVRELIATLRRQRGDVQYPGEPEARREAVRAR
                     EGIPVEPGLWSEFADWSARLGLPLPEEQ"
     gene            1975..2910
                     /locus_tag="VLN25_07605"
     CDS             1975..2910
                     /locus_tag="VLN25_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HSQ10465.1"
                     /translation="MKRDILLVSPIHGPSQAHLEAVYEVHHLWEAIDRDALLARIAPR
                     VEILVTTGGAGATRALMESLPLLRLIACFGVGVDAIDLVVAREHGIAVTNTPDVLTDD
                     VADLAIGLMLATMRGIASADRYVRASRWLRGAMPVQRKVSGKALGIVGMGRIGQAIAV
                     RAAAFGMSIGWTGPRAKDVPWRYEPDLVALARQADVLIAACPGGASTLGLISRPVLEA
                     LGSKGVFVNIARGSVVDEAALVELLSTGQLGGAGLDVFVDEPHVPEALFTLENVVLQP
                     HQASATRETRSAMGQLVIDNVAAFVTGQPLITPVT"
     gene            2918..>3414
                     /locus_tag="VLN25_07610"
     CDS             2918..>3414
                     /locus_tag="VLN25_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSQ10466.1"
                     /translation="MIPDLKGKAVLVTGASTGIGAAVARAFGAAGSTVVVHYHASEDS
                     ASAVVAAIEADGGHAHALRADATRPEQMIALVRAAVERCGRLDVLVNNVGDLVRRCPI
                     ADSTEEFFDAVVDLNVRSVVTTSRAAIPHFRSQGHGNIINTTSIAARNGGGPGSVLYA
                     SSKAFV"
BASE COUNT          551 a         1146 c         1160 g          557 t
ORIGIN      
        1 aattgcgctc cgatacgtcg ttgatcacgc agtagcccgc gacgtgatcg agcgccttgt
       61 ccctggcgac atactggacc gacgggcttt gagaagccgg gggtgctcga caggcaggga
      121 cgcctgcgcg acctgtcggg cgttgtggag catgtgacga gcgagcacct gtcggacaag
      181 ggtctggcga agctggcaaa gatcaaccca gagtcgctgc cactggtggc gggatcgccg
      241 cggctgggcg tgccgtatct ggggatcagc aagttcattg cgatcgggct gaactacgcg
      301 gatcacgcgg ccgagtcagg cgtgccggtg ccgagcgagc cgatcctgtt ctcgaaggcg
      361 acgacctgca tcaacggtcc cagcgacgac atcgtcatcc cgcgcagctc gaccaagacg
      421 gactgggagg tcgagctcgg cgtcgtgatc ggcacccagg cccagtatgt cgccagggac
      481 aaggcgctcg atcacgtcgc gggctactgc gtgatcaacg acgtatcgga gcgcaatttc
      541 cagctcgaac gtggcagcca gtgggaccgc ggcaagggct gcgacacctt cggtccgatc
      601 ggcccttacc tcgtcacgcg cgacgaagtg cccgatccgc aaaacctcga catgtggctc
      661 gacgtcaatg gccagccgat gcagcgcggc aacacacgca cgatggtctt cggcgtcgcc
      721 catctggtga gctacgtcag ccagttcatg acactgctgc cgggcgacat catcacgacc
      781 ggtacaccgc cgggcgttgg catggggcgc aagccaccgc agtatctgaa agccggcgat
      841 gtcgtgacgc tcggcatcgc aggactcggc gagcagcggc aacgcgtgcg tgcgcacgcg
      901 tgaccgcgtg gccggcgccg tttcctcccc gccggctgtg cggcatgttt ccgcctcgtc
      961 cctgacggcc tgggcgatcg cctgcctgac gcgtgtcggc gtccgagcga gcgatgccga
     1021 aacggtcgca cggtcgctgg tgcagaccag cctctggggc atcgactctc acggcattgc
     1081 ccggctcggg cattacatgg ctcgcatcaa ggcgggatcc atcgctccga taccggcgct
     1141 gcggatcaaa gagaccgggc cctgcacggc gaccatcgac ggcggacacg gcctcggcat
     1201 cgtcgtctgt accgcggcga tggcccacgc gatcggcctc gctcgatcaa acggggtcgg
     1261 catcgtcggc atcggcgaat cgtcacactg tggcgcgatc ggcctgtaca cgcgccaggc
     1321 gacggccgcc gggctgatcg gcttcgcatt cacccatgcc gattcgatcg tcgcgcccgc
     1381 cggcggcgca cagaaattgc tcggaaccaa cccgctttcg attgcgttcc cgaaccccga
     1441 cggcccgccg atctgcctcg acatggcaac gagttcgatc gcctggaacc gtgtgatgaa
     1501 cgcgcgccgc gagggtcacg ccctgcccga ggcggtcgcg atcaaccaga gcggcacgcc
     1561 gacccgcgat gcccaggagg cggcggcgct gctgccgctg ggcggaagcg aatacggcca
     1621 caagggctac gcgctggcct tgatgatcga tctgttgtgc gggccgctca acggcatgcc
     1681 gttcgccgac cgcattccgc cgatgtttgc cgacctgcag gctcggcggc acctcggctc
     1741 gctggtgatg gcgctcgacc cgatgcgatt tgcgggcggt ccgacacttg ccctgaccgt
     1801 gcgcgagctg atcgccacgc tgcgacgcca acgcggcgac gtgcagtacc ccggtgagcc
     1861 cgaggcgagg cgggaggctg tgcgcgcgcg tgaaggcatt ccggtggagc cggggttgtg
     1921 gagcgagttt gccgactggt cggctcgcct gggcctgccg ttgccggagg aacagtgaag
     1981 cgcgacattc tgctggtatc accgatacat gggccgagtc aggcgcacct cgaggccgtc
     2041 tacgaggtcc accatctttg ggaagccatc gaccgcgacg ccctgctcgc gcgcattgcc
     2101 ccgcgcgtcg aaatcctcgt gacgaccggc ggcgccggcg cgacgcgggc cctgatggag
     2161 agcctgccac tcttgcgtct gatcgcctgc ttcggcgtcg gcgtcgacgc gatcgatctc
     2221 gtggtcgcgc gcgaacacgg cattgccgtg accaacacgc cggacgtact gaccgacgat
     2281 gtcgccgacc tcgctatcgg cctgatgctc gcaacgatgc gcggcatcgc ctccgctgac
     2341 cgctatgtgc gtgccagccg ttggctgcgc ggcgcgatgc ccgtgcagag gaaggtgagc
     2401 ggcaaggcgc tcggcatcgt cggcatggga cggatcggcc aggccattgc cgtgcgggcg
     2461 gcggcattcg gcatgtcgat cggctggacc ggcccgcgcg cgaaggacgt gccgtggcgc
     2521 tacgagcctg acctcgttgc actcgcccgc caggcggacg tgctgatcgc agcctgcccg
     2581 ggcggcgcat cgactcttgg cctgatcagc cggcccgtgc tggaggctct cggttccaaa
     2641 ggcgtgttcg tcaacatcgc gcggggatcg gttgtcgacg aagccgcgct cgtcgaactg
     2701 ctgtcgaccg gacaactcgg cggcgccggg ctcgatgtct tcgtcgacga gccacatgtg
     2761 cccgaggccc tgttcacgct cgaaaacgtc gtgctgcagc cgcatcaggc cagcgccacg
     2821 cgtgagactc gcagcgcgat gggccagctc gtcatcgata acgtcgcggc gtttgttacc
     2881 gggcagcctc tgatcacccc ggtaacgtga ggacacgatg attcccgatc tgaagggcaa
     2941 agcggtgctg gtcaccggcg caagcaccgg catcggtgcc gccgtggcac gggctttcgg
     3001 cgccgcgggc agcacggtcg ttgtccatta ccacgcgagc gaagactccg cgagcgcggt
     3061 ggtggccgcg atcgaggccg acggcggcca cgcgcacgct ttgcgcgccg acgccacccg
     3121 gcccgagcag atgatcgcct tggtgcgggc cgcggtggaa cgctgtggcc ggcttgacgt
     3181 gctggtcaac aacgtgggcg atctcgtgcg ccgatgcccg atcgccgaca gcacggagga
     3241 gttcttcgac gccgtcgtcg acctcaacgt tcgctcggtc gtcacgacca gtcgggccgc
     3301 gatcccccac tttcgttcac agggacacgg caacatcatc aatacgacgt cgatcgcggc
     3361 acgcaacggc ggcggcccgg gctcggtcct gtacgcgagt tccaaggcgt tcgt
//
LOCUS       DATIRT010000332         1574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1035745, whole genome shotgun sequence.
ACCESSION   DATIRT010000332 DATIRT010000000
VERSION     DATIRT010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035745"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VLN25_07615"
     CDS             complement(<1..476)
                     /locus_tag="VLN25_07615"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HSQ10467.1"
                     /translation="MTITRFSFPTTIHFGPGARKLVGEHLRELGLRRPLVVTDRGLAA
                     LPVLTQFMQLLESGLDASVYSGVQGNPTAPQVTAGVIAYKAHRADCVIGFGGGAALDV
                     AKVVAAMAVHEGDVLEYAWDHPEVRALSHPLPPFFALPTTSGTGSEVGRSSVVSEAT"
     gene            complement(498..>1574)
                     /locus_tag="VLN25_07620"
     CDS             complement(498..>1574)
                     /locus_tag="VLN25_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSQ10468.1"
                     /translation="GVLLPQDYNGKQETYAVRNTNGTGPYRLKSYDPDQRAVLVRNPQ
                     WWGRSAKDTGNVQEAVYVVIQSDATRLAALASGQVDFVIDPPFQDVARLKADKSLTLV
                     ETIDIGTQYLGFDQSRAELQFGEARGNPFKDLRVRRAIAQSIDINLIIQKVLRGQAVP
                     AGAHLSKLVDGYVPELEQRLPFDLAAARTALKQAGYPEGFAVTLDCVNVTYRAAVCQA
                     IAAMLAQVGIRVTFQPSPSATFFPKLTQATASFFEFGWTPGGDPWASMNSIVRTHDGA
                     GSGQFNGGRYSNPKLDQLIDGIRVEPDLAVRRRMVGDALRVMHDDLPLIPLYRRKLAW
                     AMRPTVTVTQWPNDVLELRWTRIN"
BASE COUNT          264 a          509 c          527 g          274 t
ORIGIN      
        1 gtggcctcgc tgacgacgct cgagcggccg acttccgagc cggtgccgct ggtggtcggc
       61 agcgcgaaga agggcggtag tgggtgcgac agcgcgcgca cctccgggtg gtcccacgcg
      121 tattcgagga cgtcgccttc gtggaccgcc atcgcggcca ccaccttggc gacatcgagc
      181 gccgcaccgc cgccgaaccc gatcacgcag tcggcgcggt gcgccttgta cgcaatcaca
      241 cccgccgtga cctggggcgc agtcggattg ccttgtaccc ccgagtagac cgaggcatcc
      301 aggcccgatt caaggagctg catgaactgg gtcagcaccg gcagcgctgc cagtccgcgg
      361 tcggtcacca ccagcggacg acgcaggccg agttcgcgca ggtgctcgcc gaccagcttg
      421 cgcgcgccgg gtccgaagtg gatcgtggtc ggaaaggaga agcgggtgat agtcatgaaa
      481 tcgagtgccg atccggttca gttgatgcgc gtccagcgga gttcgagcac gtcgttcggc
      541 cactgtgtga cggtgactgt gggccgcatc gcccaggcga gtttgcggcg atacagcggt
      601 atcagcggca ggtcgtcgtg catcactcgc agcgcgtcgc cgaccatgcg tcgacgcacg
      661 gcgaggtcgg gttccacgcg gatgccatcg atgagctgat cgagcttcgg gttcgagtac
      721 cggcctccat tgaactgtcc gctgccggca ccgtcatgcg tgcgcacgat cgagttcatc
      781 gaagcccacg gatcgccacc cggcgtccag ccgaattcga agaagctcgc ggtggcctgc
      841 gtcagtttgg gaaagaaggt tgcacttggt gacggctgga acgtcacgcg gatccccact
      901 tgtgccagca tcgcggcgat ggcctggcat acggcggccc gatacgtgac gttgacgcaa
      961 tcgagcgtca cggcgaagcc ctcgggataa ccggcctgct tcagcgcggt gcgcgcggcg
     1021 gccagatcga agggcaggcg ttgctcgagt tcgggcacgt agccgtcgac cagtttcgac
     1081 aggtgcgcac cggcgggtac ggcctggccg cgcagcacct tctggatgat cagattgatg
     1141 tcgattgact gtgcaatcgc acggcgtacg cgcaggtcct tgaacggatt gccgcgcgcc
     1201 tcaccgaact gcaattcagc gcggctctgg tcgaagccca ggtattgcgt gccgatgtcg
     1261 atcgtctcga ccagtgtcag tgacttgtcc gccttcaacc gcgcgacgtc ctggaacggc
     1321 ggatcgatca cgaaatcgac ctgcccggac gccagcgccg ccagccgagt cgcgtccgac
     1381 tggatcacca catacacggc ctcctgcacg ttgccggtgt ccttcgcgga gcgaccccac
     1441 cactgcggat tgcgcaccag caccgcgcgc tggtccgggt cgtagctctt caggcgatag
     1501 ggaccggtgc cgttggtgtt gcgcacggca taggtttcct gcttgccgtt gtagtcctgc
     1561 ggcagcagca cacc
//
LOCUS       DATIRT010000333         4296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1232637, whole genome shotgun sequence.
ACCESSION   DATIRT010000333 DATIRT010000000
VERSION     DATIRT010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232637"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VLN25_07625"
     CDS             complement(<1..237)
                     /locus_tag="VLN25_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HSQ10469.1"
                     /translation="MSAFIAQQFVPLLFAGLFIFLLTGFPVAFSLAACGLLFGFIGME
                     AGIFPATLFQALPLRIFGIMQNDTLLAIPFFTLMG"
     gene            complement(234..815)
                     /locus_tag="VLN25_07630"
     CDS             complement(234..815)
                     /locus_tag="VLN25_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HSQ10470.1"
                     /translation="MKPWLALSRTIDMLNGHLGGLLKWLILVAVIISGGNAILRKVFN
                     IGSNAWLEAQWYLFAAVFMLGAGYAFLRNAHVRIDFISTKLSPQARNWIDIVGIVVFL
                     TPLCLLLINLSWPLFVNAWTTGEMSQNAGGLVRWPVMLLIPAGMVMLLAQAISELIKR
                     IAFLRGLIPDPLAHEAPGQKHGEETAELLEGTK"
     gene            complement(924..2342)
                     /gene="argH"
                     /locus_tag="VLN25_07635"
     CDS             complement(924..2342)
                     /gene="argH"
                     /locus_tag="VLN25_07635"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HSQ10471.1"
                     /translation="MSEPPANSPNPLGSKSSAWSGRFEEPVADFVLRYTASVGFDQRL
                     AIVDIEGSLAHAAMLAQQTILSADDLAAIERGLAQIRSEIERGEFVWRLDLEDVHLNI
                     EKRLTDLVGDAGKRLHTGRSRNDQVATDLRLWLRGEIDRIVVLVRALQRALVALADRY
                     ADTVMPGFTHLQVAQPVTFGHHLLAYVEMFDRDRERLQDARRRVNRLPLGAAALAGTT
                     FPIDRAFVAGRLGFESIVENSLDAVSDRDFAIEFSAAAALLMVHVSRLAEELVLWMSP
                     RYSFIRLPDRFTTGSSIMPQKKNPDVAELARGKSGRVVGHLMALLVLMKGQPLAYNKD
                     NQEDKEPLFDTVDTVLDTLRAFVEMVPGIEVDAAAMRRAAAEGYSTATDLADYLVRKG
                     MPFREAHEAVARAVKLAAGRGVELAELTAAELKSFSGLIDDEVRHVLTLDGSVAARDH
                     PGGTAPAQVRRQIARWKELLGG"
     gene            2537..3487
                     /locus_tag="VLN25_07640"
     CDS             2537..3487
                     /locus_tag="VLN25_07640"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HSQ10472.1"
                     /translation="MGVLLRVIGIVVALSLTAAVADADGPAEFVGQFLRISSIAAPAT
                     LTTLMLWCAQRRALPILGVPVQRAIAWLTPALVTYVLSLLFRETGTPWTPFGHALAAL
                     ALGAGVQHYYELRERAFSPAVAEAKYQALQSRIRPHFLFNSLNAVLAVIRTEPAKAER
                     MLESIAELFRAVMADTRKLVPLAQEIELCRNYVEIEQTRLGNRLAVDWQIGAYHPRAK
                     VPQLLLQPVIENAIRYGAETLPGRADIIVRVKQHGFTLEIYVSNPIAPERPQREGNQI
                     GLANIRGRLALIYDLEARIETQVRRDRFELTMTLPVEKSA"
     gene            3498..4271
                     /locus_tag="VLN25_07645"
     CDS             3498..4271
                     /locus_tag="VLN25_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HSQ10473.1"
                     /translation="MLNVMIVDDEPPARARLGQVLRDCSEQVSVQLVGEAGGGIRGLE
                     EVDRLNPDVVLLDVHMPDMNGIEVARHLAQRTNPPAVIFVTAYDEHALEAFEVRALDY
                     LMKPVRAERLVAALARAERFRTHEPRIEDLAKTIGSIRSHVTVSERGRMILVPVDDIV
                     YLRAELKYVTIRTVAREHLTEESLTSLETEFGERFVRIHRNALVARKAILGFQRVRGS
                     IDEDGESSEGHWEVLLKDVPERLPISRRQWPTVKAAAKS"
BASE COUNT          780 a         1382 c         1407 g          727 t
ORIGIN      
        1 ccccatcagc gtgaagaacg ggatcgccag cagcgtgtcg ttctgcatga tgccgaagat
       61 gcgcagcggc agcgcctgga agagcgttgc cggaaagatc ccggcctcca tgccgatgaa
      121 gccgaacagc aggccgcagg ccgccagcga gaatgcgacc gggaacccgg tcagcaggaa
      181 gatgaacagt cccgcgaaca gcagcgggac gaactgctgg gcgatgaagg cgctcatttc
      241 gtgccctcca gaagttcggc cgtttcctcg ccgtgcttct gcccgggcgc ctcatgggcg
      301 agcgggtccg ggatcagtcc gcgcaggaag gcgatgcgct ttatcagttc cgagattgcc
      361 tgtgccagca gcattaccat gcccgcgggg atcagcagca tgaccggcca gcgcaccagg
      421 cctccggcgt tctgcgacat ctcgccggtg gtccacgcat tgacgaacag tggccacgac
      481 aggttgatca gcagcaggca aagcggcgtc aggaacacga cgatgccgac gatgtcgatc
      541 cagttgcgtg cctgcggcga cagcttggtc gagatgaagt cgatgcgcac atgggcgttg
      601 cgcaggaagg cgtagccggc gccgagcatg aagaccgcgg cgaacagata ccactgtgcc
      661 tctagccagg cgttcgatcc gatattgaag accttgcgca ggatggcgtt gccgccgctg
      721 atgatgaccg caaccaggat caaccacttc agcaggccgc ccagatgccc gttgagcatg
      781 tcgatggtgc gcgacagcgc cagccagggt ttcatgtcag tgtctcctcc gtacacggcc
      841 gtccggagtc cggccgacct gtgcacccgt accgcacttt tacttttgtc ggcggattct
      901 aggcgaagca acgcgcttgc agatcaaccg cccagaagtt ctttccagcg cgcgatctgc
      961 cggcggacct gtgccggcgc ggtgccgccc gggtggtcgc gcgcggcgac ggagccgtcg
     1021 agcgtcagga cgtgccggac ctcgtcgtcg atcaaccccg agaacgactt cagctcggcg
     1081 gccgtgagtt cggccagctc gacgccgcga ccggcggcca gtttgaccgc ccgcgccacg
     1141 gcttcgtgcg cctcgcggaa cggcatgccc ttgcgcacga ggtaatcggc caggtcggtc
     1201 gccgtcgaat aaccttccgc ggcggcccgt cgcatcgcgg cggcatcgac ttcgattccc
     1261 ggcaccattt cgacaaaagc ccgcaaagtg tccagcacgg tatcgacggt gtcgaacagc
     1321 ggctccttgt cctcctggtt gtccttgttg taggcgaggg gctgcccctt catcagcacc
     1381 agcagggcca tcaggtggcc gacgacgcgt ccgcttttgc cgcgcgcaag ttccgcaacg
     1441 tccggattct tcttctgcgg catgatcgac gaaccggtcg tgaagcgatc cggcaggcgg
     1501 atgaagctgt aacgcgggct catccacagc accagttcct cggccagccg ggagacatgc
     1561 accatcagca gcgcggcggc cgcactgaac tcgatcgcga agtcgcggtc gctgacggcg
     1621 tcgagcgagt tttcgactat cgactcaaac cccagccgcc cggcgacgaa ggcgcgatca
     1681 atcgggaacg tcgtgccggc cagtgccgcc gcacccaggg gcaggcgatt gacccggcgg
     1741 cgagcatcct gcagccgttc acgatcgcga tcgaacattt cgacataggc cagcaggtga
     1801 tggccaaagg tcaccggctg cgcaacctgc aggtgcgtga atccgggcat tacggtatcg
     1861 gcgtagcggt cggccagtgc gacgagtgcg cgctgcagcg cccgcacgag caccacgatg
     1921 cggtcgatct cgccacgcag ccacaggcgc aggtcggtgg cgacctggtc attacgcgag
     1981 cgaccggtgt gcaggcgctt gccggcatcg ccgaccaggt cggtcaatcg cttttcgatg
     2041 ttcaggtgca cgtcttcgag gtccaggcgc catacgaact cgccgcgctc gatctcgctg
     2101 cggatctgcg ccaggccccg ctcgatcgcg gcgaggtcgt ctgcggagag gatcgtctgc
     2161 tgcgccagca tcgctgcgtg ggccagcgag ccttcgatgt cgacgatggc caggcgctga
     2221 tcgaagccca ccgaggcggt gtagcgcagc acgaaatccg ccaccggttc ctcgaaacgg
     2281 ccggaccagg cgctgctttt cgaacctagg ggattggggg agttcgcggg gggctctgac
     2341 ataatcatcc ggagaggaag gtggcctgtt gccacgggga gggcgacctt gtgccttgca
     2401 ccgaggcctg tcgcatctgt ctgcggtcgc cgattatagg tttgtgaagc cgtcgccacc
     2461 tgcgataaaa ggtccgctcg cccgcgcgga aacggcgatc ccgacgccgc gccgattgat
     2521 tcccgacggc tgcaacatgg gggtgttgct gcgggtcatc ggcatcgtgg tggccctttc
     2581 gctgaccgct gccgtggccg acgccgacgg gccggcagaa ttcgtcgggc agttcctgcg
     2641 catctcgtcg atcgcggccc cggcgacact gacgactctg atgctgtggt gtgcgcaacg
     2701 gcgcgcgctg ccgatcctgg gcgtgccggt acagcgggcg atcgcgtggt tgactccggc
     2761 gctcgtgacc tatgtgctgt cgctgctgtt ccgcgagacc ggcacgccgt ggacgccgtt
     2821 cgggcatgcg ctggctgctc ttgcactcgg tgccggcgtc cagcactact acgaactgcg
     2881 cgagcgcgcc ttttcacccg cggtggccga ggcgaagtac caggcgctgc agtcgcgcat
     2941 ccggccgcac ttcctgttca acagcctgaa cgccgtgctc gcggtcatcc gtacggagcc
     3001 cgccaaggcc gagcgcatgc tggaaagcat tgccgaattg ttccgcgcag tgatggcgga
     3061 tacccgcaaa ctggtgccgc tggcacagga aatcgagctg tgtcgcaact atgtggaaat
     3121 cgagcagacc cggctcggaa ataggctggc ggttgactgg caaatcgggg cataccaccc
     3181 ccgggcaaag gtgccacagt tgctactgca gccggtgatt gaaaacgcga tccgctatgg
     3241 ggccgaaacg ctgcccggac gcgccgatat catcgtccgc gtgaagcagc acggtttcac
     3301 gctggaaatt tatgtgagca atccgatcgc acctgagcgt ccccagcgcg aaggcaacca
     3361 aatcggactg gcgaacatcc gcgggagact ggctttgatt tatgacctcg aagcgcgcat
     3421 cgaaacccag gtcaggcgcg accgcttcga gctgacaatg accttgccgg tcgagaaaag
     3481 cgcctgaagg gccagtcatg ttgaacgtga tgatcgtgga cgacgaacct ccggcgcgag
     3541 cgcgcctggg gcaagtcctg cgcgactgtt cggagcaggt gtctgtccag ctagtcggcg
     3601 aggccggcgg cggcatccgc ggactggaag aagtcgaccg cctcaatccg gacgtcgtgc
     3661 tgctcgacgt gcacatgccg gacatgaatg gcatcgaagt cgcgcgccat ctggcgcagc
     3721 ggaccaatcc gccggcagtg atcttcgtca ccgcctacga cgagcacgcg ctggaagcgt
     3781 tcgaggtgcg cgcgctcgac tacctgatga agcccgtgcg tgccgagcga ctcgtcgctg
     3841 cactggcccg cgccgaacga tttcgcacgc atgagccgcg catcgaggac ctcgccaaga
     3901 cgatcggctc gatccgctcg catgtaaccg tgtccgaacg tggccgcatg atcctcgtgc
     3961 cggtcgacga catcgtctac ctgcgcgctg aactgaagta cgtgacgatc cgtacggtcg
     4021 cgcgcgagca cctgaccgag gaatcactaa catcgctcga aaccgagttc ggcgagcgct
     4081 tcgtccgcat ccaccgcaat gcgctggtgg cgcgcaaggc catcctcggc ttccagcgcg
     4141 tgcgcggcag catcgacgag gacggagaat cgtccgaggg ccactgggaa gtcctgctga
     4201 aggacgttcc cgaacgcctg ccgatcagcc gccggcaatg gccgacggtg aaggcggcgg
     4261 cgaagtcgta gcgtcgctcg tgtacgcgat cggcta
//
LOCUS       DATIRT010000334         3576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_642558, whole genome shotgun sequence.
ACCESSION   DATIRT010000334 DATIRT010000000
VERSION     DATIRT010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642558"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..1149
                     /locus_tag="VLN25_07650"
     CDS             112..1149
                     /locus_tag="VLN25_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSQ10474.1"
                     /translation="MSGTAIRLERCGKTFADGTRALDPVDLEFTAGETVVLLGPSGCG
                     KTTTLRIVAGLELPDAGGRVLFDGADVTAVPIERRNVGMVFQSYALFPNMSVEENVAY
                     GLKVRGMPAADRDQRASEMLAMMQIAPLAARSIDQLSGGQRQRVALARALAPRPRVLL
                     LDEPLTALDARLRDTLRVEINRLLRGLGITTVYVTHDQAEAMALGDRIVVMDHGRIAQ
                     IGTPRQIYEAPSTRYVADFIGTMNIVHGDIRAGALHFGGGVLPLRDGATEGSGEAMFR
                     PEDLLLAASGGPTQFGGQVLASLFQGDHTRLEVDIGTGKPIVARIAQRRDFVIGERLS
                     FTLSTPAIQMP"
     gene            1165..1980
                     /locus_tag="VLN25_07655"
     CDS             1165..1980
                     /locus_tag="VLN25_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HSQ10475.1"
                     /translation="MLLAQISDMHIKPQRRLAYGVVDTAAMLEQCVAAILKLKQRPDA
                     VIATGDLVDLGRPEEYGLVREILAPLSSMPLYLLPGNHDERGALRASFPDHDYLRQWE
                     PFVQYAIEDHSLRIVGLDTVIPGQGGGELCSARLDWLDRTLAQSDRPTVVALHHPPFV
                     TGIGHMDRVCLTNTDALAQVLLRHPQVARVIAGHLHRPIICQFGGTVASVCPSPAHLV
                     ALDLSPDAPDNFVMEPPGFQLHVQTDSGLVTHTAYIGEFAGPYPFREGGRLID"
     gene            2119..2826
                     /locus_tag="VLN25_07660"
     CDS             2119..2826
                     /locus_tag="VLN25_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HSQ10476.1"
                     /translation="MTDFVLVHGAWHGAWCWRKILPSLWAKGDRAFAVTLTGVGEREH
                     LMSTSIRLATHINDVVSVIETEELPRLVLVGHSYAGLLITAVADRYAERIVRLVYLDA
                     IVPHSGESWSSSHNESARQARRADIARDGVIPPPPASAYGLTGEDAAWVDRRQKPHPG
                     GVYEDVLQFDESRVASLSRTFVDCTSPALPTIEKSRQRVRSEPGWKVIEIATGHDPMI
                     SAPTQLLDILHQAGRSN"
     gene            complement(2986..3387)
                     /locus_tag="VLN25_07665"
     CDS             complement(2986..3387)
                     /locus_tag="VLN25_07665"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HSQ10477.1"
                     /translation="MLKTFRIVLPLVLAALPVAPSLAAENQPVSKRQQYVYVLKLATR
                     MQEANAWTDKENGVIGQHFARLAKATDTGQVILAGRTTEALDKTFGLVIFEAESDEAA
                     RLFMEADPAVVANIMSATLHPYSVALYRKTR"
BASE COUNT          640 a         1113 c         1158 g          665 t
ORIGIN      
        1 cgccagtgcg gcgccggccc gttgaggaaa agacatgagc gccgcacggc cgctccgaag
       61 gcgctcgctc cccctcgggg ggacggcgcg cggcaacaag ggggccacag gatgagtggc
      121 accgcgatac ggctcgaacg ctgcggcaag acctttgccg acggcacgcg cgccctcgat
      181 cccgtggacc tggagttcac cgcaggggaa accgtggtgc tgctgggccc cagcggctgc
      241 ggcaagacca ccacgctgcg catcgtggcg ggactggaat tgccggatgc cggaggtcga
      301 gtccttttcg acggcgccga cgtcactgca gtaccgatcg agcggcgcaa cgtcggcatg
      361 gtgttccaga gctatgccct gtttcccaac atgtcggtcg aagagaatgt tgcgtacggc
      421 ctcaaggtgc gcggcatgcc cgcagcggat cgagaccagc gtgcgagcga gatgctggcg
      481 atgatgcaga tcgcgcctct ggcggcccgc agcatcgatc agctctccgg cggccagcgc
      541 cagcgggtgg cgctggcgcg tgcgcttgca cctcgaccgc gtgtgttgct gctcgacgaa
      601 cctctgactg cgctggatgc tcgcctgcgc gacacgctgc gagtggaaat caaccggctg
      661 ctacgcgggc tgggaatcac gaccgtctat gtgacgcacg accaggccga agcgatggcg
      721 ctgggcgatc gcatcgtggt gatggatcac ggccgcattg cacagatcgg caccccccgc
      781 cagatctacg aagcgccgag cacgcgctac gtggccgatt tcatcggcac gatgaacatc
      841 gtgcacggcg acattcgcgc tggagcgctg cacttcggag gcggggtcct gcctctgcgg
      901 gacggggcca ccgaaggcag cggtgaagca atgttccgtc cagaggacct gttgctcgcc
      961 gcgtctggcg gaccgacgca gtttggcggc caggtgctgg cgagtctttt ccagggcgac
     1021 cacacacggc tggaagtcga tatcggaacg ggcaagccga tcgtggcgcg catcgcccag
     1081 cggcgcgact tcgtgattgg cgagcggctg tcctttacgc tgtcgacgcc cgcgatccag
     1141 atgccgtaag cccgagacac ttccatgctg ctcgcccaga tttccgacat gcacatcaag
     1201 ccgcagcggc ggcttgccta cggcgtggtc gataccgccg cgatgctgga gcaatgcgtc
     1261 gcggcgatcc tgaagcttaa gcagcggcca gatgccgtta tcgccaccgg ggatctggtg
     1321 gatcttgggc ggccggagga atacggactg gtgcgggaga tactcgctcc gctgtcatcg
     1381 atgccgctgt acctgctgcc aggcaaccat gatgaacgcg gggcgctgcg cgcgtccttt
     1441 ccggatcacg actacctgcg ccaatgggag cccttcgtgc agtacgcaat cgaggatcac
     1501 tcgctgcgta tcgtcgggct ggacacggtg atcccgggcc agggcggtgg cgagctctgc
     1561 tcagcccggc tcgactggct cgaccgcacg cttgcgcaga gcgatcgtcc gacggtggtc
     1621 gcgctgcatc acccgccctt tgtgaccggg atcggtcaca tggatcgcgt gtgcctcacc
     1681 aacaccgatg cgctggctca ggtgctgttg cgccatcccc aggtggcgcg cgtcatcgcg
     1741 ggccacctgc accggcccat catctgtcag ttcggcggaa ctgtcgccag cgtgtgcccg
     1801 agccccgcgc acctggtggc gctcgacctg tcgcccgacg cacccgacaa cttcgtgatg
     1861 gagccgcccg gctttcagtt gcacgtgcag actgacagcg gcctggtgac ccatacagcg
     1921 tatatcggtg agtttgcagg cccgtacccg ttccgcgagg gcgggcgctt gatcgactaa
     1981 tgaccggccc attccaggga gaagtcttga caggcatcag gatcacgcga gccgagctcg
     2041 gtcgtgccgt cggaagtggc accccgtctt tcattgcgag tggtggcatg atcgaaagct
     2101 ctgcacgggg ccaatggcat gactgacttc gttctcgttc atggcgcatg gcatggcgcc
     2161 tggtgctggc gaaagatcct gccgtcactc tgggctaagg gcgatcgcgc atttgcagtg
     2221 acgctaacag gggtcggcga acgggaacac ctgatgtcca cttccatccg actcgctact
     2281 cacatcaacg atgtggtgtc agtgattgag acggaagagc tgccgcgcct cgtccttgtg
     2341 ggtcatagct atgccgggtt gttgatcaca gccgtggctg atcgctatgc ggaacgcatt
     2401 gtccggctgg tctatctcga tgccattgtt ccccacagcg gcgagagttg gtcgagttcc
     2461 cacaacgaaa gcgcgcgcca ggcccgtcgt gcagacatcg cgcgggacgg cgtcatccca
     2521 cctccgcccg cgtcggccta tggcctgacc ggcgaggatg cggcgtgggt ggaccgacgc
     2581 cagaagccac atccgggcgg tgtgtatgaa gacgtgctgc agttcgacga gtcgcgtgtg
     2641 gcaagcctgt ctcgtacttt cgtggactgc acctcgccgg cacttccgac gatcgaaaaa
     2701 tctcgtcagc gagtgcgttc cgaaccgggc tggaaggtga tcgaaatcgc caccggccac
     2761 gatccgatga tcagcgcacc gactcagtta ctggacatcc tgcatcaggc aggacgttca
     2821 aactgaggct ggcgcgtgtc gctggagtcg gccaaaacca ggcccggccc ctgagtccga
     2881 acccagctga atagcggacg gacatgctgg cttggagcag acttacgccc gcatctgttg
     2941 ttggcctgac agcgacatcg catgaccatg cgaatcgagt acttgctatc gggtcttgcg
     3001 gtagagcgca accgagtacg ggtgcagtgt ggccgacatg atgttggcca ctaccgcggg
     3061 atcagcttcc atgaagagcc tggcggcttc atcgctttcg gcctcaaaga tcacgaggcc
     3121 gaaagtcttg tccagggctt cggttgtgcg ccccgcgaga atgacctgac ccgtgtcagt
     3181 tgccttcgca aggcgagcga agtgctgtcc gatcacgcca ttctctttat ccgtccaggc
     3241 attagcttct tgcatgcgcg tagccaactt cagaacgtag acgtactgct gacgcttgct
     3301 caccggctgg ttctcggccg caaggctggg ggccacaggc aaggcggcca agactaacgg
     3361 aagcactatt cggaacgtct tcagcatttt tctgcctttc aagtcacgta cgcgaatgca
     3421 agttgcggca agaggctcac ggggctgcgt catacgccgc gcgacaaggt tgtctccttg
     3481 gcatgtcagg tgtggctaca gcggccaatg ttcgctgcga gtgcgcttcg gcagaacgaa
     3541 ctccatcaga tcgaaggcgg cgtacatcag tcgagt
//
LOCUS       DATIRT010000335         2628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1724687, whole genome shotgun sequence.
ACCESSION   DATIRT010000335 DATIRT010000000
VERSION     DATIRT010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1724687"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1185)
                     /locus_tag="VLN25_07670"
     CDS             complement(58..1185)
                     /locus_tag="VLN25_07670"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HSQ10478.1"
                     /translation="MLRTVVVLLPLLFVVDAASSATTTREHRVRIVTVTEGLERPWGL
                     AFLPDGRMLVTERPGRMRVVSREGKLDPTPVAGLPRVDAQSQGGLLDVTLHPQYSSNG
                     WIYWSYAQRDATGNWTEVARGKLAGGPGNWRMESVEVLFRMAPKSSGGHHFGSRIVFD
                     RSGLMYVTLGERGEMQRAQRLDDHGGKILRLTDDGKPAPGNPFLNDPKAQPEIYTFGH
                     RNVQGAAIHPVSGALWASEHGPQGGDELNLIRAGVNYGWPVITYGVNYGIGTKIGEGT
                     AKAGMEQPVKYWTPSPAVAGITFYEGDRFPKWRGDLLVATMKADALIRVRLNGERFVE
                     DEFMLKGELPRVRDVRTGPDGFVYLLTDEKNGAILRLEPAN"
     gene            1332..1949
                     /locus_tag="VLN25_07675"
     CDS             1332..1949
                     /locus_tag="VLN25_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HSQ10479.1"
                     /translation="MYHGERFNSSTHLVGTALAITGSVILITRAALDGDPWKIVSFSL
                     FGSMLIVLYAISTLYHSIRATRAKAVLRRLDHCAIYLLIAGTYTPFTLVTLRGALGWT
                     LFGTIWAMAAVGIVRAWRHRGEGDPSVWPYLVMGWLGICAAVPLVARLGAGGMLWLAA
                     GGLLYTAGILFFINDTRWRHAHGVWHLFVLGGSAAHFIAVLRYVR"
     gene            1946..>2628
                     /locus_tag="VLN25_07680"
     CDS             1946..>2628
                     /locus_tag="VLN25_07680"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HSQ10480.1"
                     /translation="MTDRSYLFVPGNRPERFESACRAGADVVLVDLEDAVAAADKATA
                     RRAVGQWLTSGGAAYLRINGADTAAFRDDLLLLGLPGVQGVMLAKTEDNEPIAAIRLS
                     APALPVIPLIESARGLSNVNLIATALGVVRLAFGSVDFQLDLGIGGDHDELLMARSQL
                     VLASRVARLLPPIDGVTMALDDTEALLRDVAYARRLGFGGKLAVHPRQVPAINEGFSP
                     SPADLEWARR"
BASE COUNT          435 a          875 c          799 g          519 t
ORIGIN      
        1 caacgaggcg ggtttcacga gaacagcgga ctactctgtt ccctattgca acgcacgtca
       61 gttcgccggc tctagccgca ggatggcgcc gttcttttca tcggtcagca gatagacaaa
      121 gccgtccggt ccggtgcgta cgtcgcgcac gcgcgggagt tcgcccttga gcatgaactc
      181 gtcttcgaca aaccgctcgc cgttcagtcg cacgcggatg agggcatcgg ctttcatcgt
      241 ggccacgagc aggtccccgc gccatttagg gaaccgatcg ccttcgtaga atgtgatacc
      301 ggcgaccgcc ggtgacggtg tccagtactt gaccggctgc tccatgccgg ccttcgcggt
      361 gccctcgcca atcttggtgc cgatcccgta gttcacgccg tacgtgatga ccggccagcc
      421 atagttgacg ccagcccgga tcaggttcag ttcgtcgcct ccctggggac cgtgctcgct
      481 tgcccagagg gcaccgctga cgggatggat ggctgcgccc tgaacattgc ggtgtccgaa
      541 cgtataaatc tcgggctggg ccttgggatc gttcaggaat ggatttcccg gtgccggctt
      601 gccgtcgtcg gtgaggcgca ggatcttgcc gccatgatcg tcaagtcgtt gcgcgcgctg
      661 catctcgccg cgctcgccca gcgtcacgta catcaggccc gagcggtcga acacgatgcg
      721 cgagccgaag tggtgaccac cgctgctctt cggagccata cggaacagga cctcgaccga
      781 ttccatgcgc cagttgcccg gtccgcccgc caacttgccg cgcgccacct cagtccagtt
      841 gcccgtcgcg tcccgctgcg cgtagctcca gtagatccac ccgttcgatg aatactgcgg
      901 atgcagtgtc acgtcgagca ggccgccctg gctctgtgcg tccacccgcg gcagtcccgc
      961 gactggcgtg ggatcgagct ttccttcgcg gctgactacg cgcatgcgcc caggtcgctc
     1021 ggtgaccagc atgcgaccgt ccggaaggaa tgccagcccc cagggacgct ccagcccctc
     1081 ggttaccgtg acgatccgca cccgatgttc tcgcgtggtc gtggcactgc tggccgcatc
     1141 gaccacgaat agcagcggaa ggagcaccac tacggtacgc agcatgatca tttaatggtc
     1201 taagtccgtt aaaagtgtgg gaattctgct cgtgacgcgc cagccgagag tgacacactt
     1261 ccacctgtaa aattccacgc acacgtagcc tatagtcaac ccacacacag caaagttccg
     1321 cttccatcct cgtgtatcac ggcgaacgat tcaacagcag cacccatctc gtcggcaccg
     1381 cactcgcgat caccggatcg gtaatcctga taactcgcgc cgcgctcgac ggcgacccct
     1441 ggaagatcgt cagcttcagc ctgttcggct cgatgctgat cgtgctctac gccatttcga
     1501 cgctgtatca ctcgatcagg gcgacgcgtg cgaaggcggt gctgcggcgg ctcgatcact
     1561 gcgcgatcta tctgctgatt gctggaacct acacaccctt cacgctcgtg acactgcgcg
     1621 gcgccctggg ctggactctg ttcggaacga tctgggccat ggcagcggtc ggcatcgtgc
     1681 gcgcgtggcg tcatcgcggc gaaggcgatc cttccgtttg gccctatctc gttatgggat
     1741 ggctcgggat ctgcgccgcg gtgccgctgg tcgctcggct cggtgccggc ggaatgctgt
     1801 ggctcgccgc aggcggactc ctttacaccg cagggatact gttcttcatc aacgacactc
     1861 gctggcgcca tgcccatggc gtctggcatc tgtttgtcct cggcggcagt gcggcgcact
     1921 tcattgcggt gctgcgctac gtcaggtgac tgaccgctct taccttttcg taccgggcaa
     1981 ccgacccgag cgcttcgagt cggcgtgccg tgcgggcgcc gacgtggtcc tcgtagatct
     2041 cgaagacgca gttgccgctg ccgacaaggc aactgcgagg cgagcagtcg gacaatggct
     2101 cacctccggc ggtgccgcgt acctgcgcat caacggcgct gataccgctg cgttccggga
     2161 cgacctgctg ttgctcggtc tgccgggcgt acaaggcgtg atgctggcca agaccgagga
     2221 caacgagccg atcgccgcga tccggctttc cgcgcccgcc ctacctgtca tcccgttgat
     2281 cgaaagtgcg cgtgggctct cgaacgtgaa tctgatcgcc accgcactcg gagtcgtgcg
     2341 gctggcgttc ggatcggtcg atttccagct cgatctcggc atcggcggag atcacgatga
     2401 actcctgatg gcacgttcac aactggtgct ggcatcccgg gtcgccagac tgctgccgcc
     2461 gatcgatggc gtcacgatgg cactcgacga cacggaagcg ctgttacgcg acgtggccta
     2521 cgcgcgccgc ctgggctttg gaggcaagct cgccgtccac ccccgccagg tccccgcgat
     2581 caacgagggc ttctcgccat cgccggctga cctggaatgg gcgcgtcg
//
LOCUS       DATIRT010000336         5233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1919883, whole genome shotgun sequence.
ACCESSION   DATIRT010000336 DATIRT010000000
VERSION     DATIRT010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5233
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1919883"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VLN25_07685"
     CDS             <1..423
                     /locus_tag="VLN25_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10481.1"
                     /translation="QNGSITLYGAKSGAIVADTTNDVTIQLQPTGTVKGIVKRADGDA
                     VPLAVGAEALGEWLGERARRGATTIISDSGFDRWALAELFGGENLPANVRWQRVPVAY
                     EALDEATQRLNLRRHHALDDARALRQLVLEAQVPARPA"
     gene            complement(521..949)
                     /locus_tag="VLN25_07690"
     CDS             complement(521..949)
                     /locus_tag="VLN25_07690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HSQ10482.1"
                     /translation="MPVRVVIDTNVLLDFWVFDDPRAAPLRLAVESGEVGALRSGPSV
                     DEFSDVLGRPVFGVSNEHRCTILRHWDRLAAPVERVHAAPFACTDPHDQKFLDLALTA
                     RADWLVTKDKALLKLARRARRDGLQIVDPADAVALISQRA"
     gene            complement(958..1347)
                     /locus_tag="VLN25_07695"
     CDS             complement(958..1347)
                     /locus_tag="VLN25_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HSQ10483.1"
                     /translation="MRDADRPSSARNVLGGTLAQCCDDPATGFYRDGFCHTGPQDVGS
                     HTVCARMSHEFLEFSLQQGNDLVTAQPEFGFPGLRAGDRWCLCVARWHEALEAGVAPK
                     VILASTHEKALAVVTLEQLTQHALDVQ"
     gene            complement(1353..2000)
                     /locus_tag="VLN25_07700"
     CDS             complement(1353..2000)
                     /locus_tag="VLN25_07700"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HSQ10484.1"
                     /translation="MSTLRPFRTHAGVLLAMVVCTACITVDVGHDTGLQAQYRIVDSG
                     PAPTARSRPLGRELLISPIPTSAIDDSFSLAFSRAPNQRAAYQFATWSDRPSNRLAQL
                     LVERLASRGAFNSVALLGRGVGGDLQVNLTVNDFYHDAGTNPGTANIAVTAELIDRAT
                     RKLIARQRFTASSPVQQANAASAAAALGQASTLILDQLVTWVEATAAPAALATVR"
     gene            complement(2003..2941)
                     /locus_tag="VLN25_07705"
     CDS             complement(2003..2941)
                     /locus_tag="VLN25_07705"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HSQ10485.1"
                     /translation="MEADAKYTYVGLAVIALIAALIGGIVWLKSAGGRNEFNYYTIYF
                     EKQPLDGLQIGADVLMRGIKVGRVEDYALTAANINRVRVLIRIDRRTPVKTSTVAVVT
                     RSFVTGIAKIDLQTPDPPGPTLTAILDDEPYPVITEGTSNIDAIANRLNLVGETAVKT
                     LESLNEVLKPENRAAIDQTLANLRDLTGGLNQRLKELDRTLTTVNRAADDLGRSSTRI
                     AGVADSAGQDLRPALHQAERTMKDISGATIALEQQAAQLSQAVSSTVLSSGDQLAIAI
                     SELKSTGETLNRLLDRLQDPRAALLGPTPQQRGPGE"
     gene            complement(2949..3749)
                     /locus_tag="VLN25_07710"
     CDS             complement(2949..3749)
                     /locus_tag="VLN25_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSQ10486.1"
                     /translation="MADAANAAAAGPVVIEIDSIVTRFGEQSVHDGVSFNVHKGTLVA
                     LIGGSGTGKSVLLREIIGLQRPTSGRIRLLGTDIWSASEEEVNDVRRRFGMMFQDGAL
                     FSSLTVAQNIAVPLTEHASVPPALLDSLIHLRLAQAGLGTDVAGKMPSELSGGMRKRA
                     AIARALALEPEVLFLDEPTSGLDPTTARAFDDMVRTLVDDLGITVFLVTHDLDTLLTV
                     VDRIVVLARGKVLADGAGREVMHVDDPWIKSYFSVRTMVSGSAPSHLE"
     gene            complement(3749..4885)
                     /locus_tag="VLN25_07715"
     CDS             complement(3749..4885)
                     /locus_tag="VLN25_07715"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSQ10487.1"
                     /translation="MPAEPAHPATPPGARLDDGADSVLHLSGAWRLGALPTIVDALDA
                     LSLPPKLSVDGTAVTEIDTAAALALLDHLQRSGIDATTVELSGFAPNEGRIVEQVRQR
                     LAVINAPVPRHRHGLLAQIGRHTLTIRDLVAGHLTYVGMVFDGLFRGVLSPTEVRTRE
                     LTVQLEQVCLNAIPVVSLVTLLIGVVVAYLLGLQAEQYGANIFVVDGVGIGATREFAP
                     IIVAVIVAGRSGAAFTAQLGSMRLNEETDAIRTLGLSPLDVLVMPRVFALMIALPLLV
                     FVGDLMSLVGAMLIAGPMLDITPTTFLARLQDNLAIKHVYVGLAKAPMFALVIATIGC
                     RMGMMVDRDTRALGMATTSTVVQSIVAVILLDAAFAVLFQELGI"
BASE COUNT          890 a         1693 c         1792 g          858 t
ORIGIN      
        1 cagaacggca gcatcacgct gtacggcgcg aagtccggcg cgatcgtcgc cgacaccacc
       61 aacgacgtca ccatccaact gcagcctacc ggcaccgtga agggcatcgt caagcgcgcc
      121 gacggcgatg cggtgccgct ggccgtcggc gccgaagcac tgggcgagtg gctcggcgag
      181 cgtgcccgcc ggggagcgac gacgatcatc agtgactcgg gcttcgatcg ctgggctctg
      241 gccgaactgt tcggtggcga aaacctgccc gcgaacgtac gctggcaacg cgtgccggtg
      301 gcctacgagg cgctcgacga agcgacgcag cgactgaacc tgcgtcggca ccacgcgctg
      361 gatgacgccc gcgcactgcg ccagctagtg ctcgaggcac aggtgcccgc gcggccggcc
      421 taaccgcaat ggagttgggc ggcgccgtgc acgagtcccc ggccgcaccg cgtcgatggc
      481 ctcattgtcg ccatcaccgc cgacagcgca gccgtctcgg tcatgcgcgc tggctgatca
      541 gcgcgaccgc atcggccgga tcaacgatct gcaggccgtc gcgccgcgcg cgccgcgcga
      601 gtttcaacaa agccttgtcc ttggtgacca gccagtcggc cctcgccgtc aacgccaggt
      661 cgagaaactt ctggtcgtgc ggatcggtgc aggcgaaagg tgcggcgtgc acccgttcga
      721 ccggcgcggc gagccgatcc caatgccgca ggatcgtgca tcggtgttca ttcgagacgc
      781 cgaacaccgg tcggccgagc acgtcactga attcgtccac cgatgggccg ctacgcagcg
      841 cgccgacttc gccgctttcc acggcaaggc gcaagggcgc cgcgcggggg tcatcgaaga
      901 cccagaaatc cagcagcacg ttggtgtcga tgacgacgcg gactggcacc cgcgagttca
      961 ttggacgtcg agcgcgtgct gcgtgagttg ctcgagcgtc actacggcga gcgccttttc
     1021 gtgtgtcgag gcgaggatga cctttggtgc aacacccgcc tccagggcct cgtgccagcg
     1081 ggcgacgcac aggcaccagc ggtcgccggc acgcagcccc ggaaagccga actccggttg
     1141 tgctgtgacc aggtcgtttc cttgctggag cgaaaactcc aggaactcgt ggctcatgcg
     1201 tgcgcacacc gtgtgcgacc cgacgtcctg cggccccgtg tggcagaaac cgtcgcggta
     1261 gaacccggtt gcgggatcat cgcagcactg cgccagcgtg ccgcccagga cattgcgtgc
     1321 cgaggaaggg cggtcggcgt ctcgcatcga tgtcagcgaa cggtcgccag tgccgcgggg
     1381 gcggcagtcg cttcgaccca ggtgaccaac tggtcgagga tcagcgtgct cgcctgtccg
     1441 agcgcggcgg ccgcggaggc ggcgttggcc tgctggacgg gcgacgaagc ggtgaatcgc
     1501 tgcctcgcga tcagctttcg tgtcgcgcgg tcgatcagct cagcggtcac tgcgatgttg
     1561 gcggtgcctg ggttggtgcc tgcgtcgtga tagaaatcgt tgacggtcag attgacctgc
     1621 aggtccccgc cgacgccgcg ccccagcagg gcgaccgagt tgaacgcgcc gcgcgatgcc
     1681 agacgctcga ccagcagttg cgcgagacga ttcgacggcc ggtcgctcca ggtcgcgaac
     1741 tgatacgccg cccgctgatt gggcgcgcgc gaaaacgcca gcgaaaacga atcgtcgatg
     1801 gcactggtgg gaatcggcga gatcagcagc tcgcgtccga gcggccgcga acgggccgtc
     1861 ggggccgggc cggagtccac gatccgatat tgggcctgca gtccggtgtc gtggccgaca
     1921 tcgacggtaa tgcaggcggt gcaaaccacc atcgccagca ggacacctgc gtgtgtgcgg
     1981 aacgggcgca aggtcgacat ggtcactctc ccgggccgcg ttgctgcggc gtcggtccta
     2041 gcagcgcggc gcgcgggtcc tgcagccgat cgaggagtcg attgagagtc tcgcccgtcg
     2101 acttgagttc gctgatggcg atcgccagct gatcgccgct cgacaggacc gtagagctga
     2161 cggcctgcga cagctgcgca gcctgctgct cgagggcaat ggtcgcgccc gaaatgtcct
     2221 tcatcgtccg ctcggcctgg tgtaacgcgg gccgcaggtc ctgacccgcg ctgtcggcga
     2281 ccccggcgat gcgcgtgctc gagcggccca agtcgtcggc ggcgcgattg acggtcgtga
     2341 gtgtgcgatc gagttccttc agtcgctggt tcagcccgcc ggtgaggtcg cgcaggttgg
     2401 ccagggtctg gtcgatggcc gcccggttct cgggcttcag cacctcgttc aggctttcga
     2461 gcgtcttgac ggcggtctca cccaccagat tgagccggtt ggcgatggcg tcgatgttcg
     2521 aagtgccctc ggtgatcacg gggtacggct cgtcgtcgag aatcgccgtc agcgtgggac
     2581 ccggcggatc gggtgtttgc aggtcgatct tcgcgatgcc ggtcacgaag ctgcgtgtca
     2641 ccaccgcaac ggtgctggtc ttgaccggcg tgcggcgatc gatgcgaatc agcacgcgca
     2701 cgcggttgat gttggccgct gtcagcgcat agtcttccac gcgtccgacc ttgatgccgc
     2761 gcatcagcac gtcggcgccg atctgcaggc cgtcgagcgg ctgcttctcg aagtagatcg
     2821 tgtagtagtt gaattcgttg cgcccaccgg ctgacttcag ccagacgatg ccgccgatca
     2881 gcgccgcgat cagcgcgatg accgctagac ctacgtaggt gtacttggcg tccgcttcca
     2941 tggctcgatc attctaagtg ggaaggtgcc gatcccgaca ccatggttcg gaccgagaaa
     3001 tacgacttga tccacgggtc gtcgacgtgc atcacttcgc gcccggcacc gtcagccagg
     3061 actttgccgc gcgcgagcac aacgatgcgg tcgacgacgg tcagcagcgt gtcgaggtcg
     3121 tgggtgacga gaaacaccgt gatgccgaga tcgtcgacca gcgtccgcac catgtcatcg
     3181 aaggcgcggg cggtcgtcgg gtccagccct gaggtgggtt cgtcgagaaa gaggacctcg
     3241 ggttccagcg ccaaggcacg cgcgatcgcc gcgcgcttgc gcatgccgcc cgacagctcg
     3301 ctcggcattt tcccggccac gtcggtgccg aggcccgcct gcgcaagccg caagtgaatc
     3361 aggctgtcga gcagggcagg gggcacgctg gcgtgctccg tgagcggcac cgcgatgttc
     3421 tgtgcgaccg tgagcgacga gaagagcgcc ccatcctgga acatcatgcc gaagcgccgt
     3481 cgtacatcgt tgacctcctc ttcggaagcg gaccaaatgt ccgtgcccag cagtcggatg
     3541 cgaccggacg tcgggcgctg caggccgatg atctcgcgca gcaatacaga tttgccggtg
     3601 ccactgccgc cgatgagggc aaccagcgtg cccttatgca cattgaagct gacgccatcg
     3661 tgcaccgact gttcgccaaa gcgcgtcacg atcgagtcga tctcgatgac cacgggtccg
     3721 gcggctgccg cgtttgccgc gtctgccatc agattccgag ctcctggaac agaaccgcaa
     3781 acgccgcgtc gagcaggatc acggcgacaa tgctctgcac gacggtcgac gttgtcgcca
     3841 tcccgagcgc acgcgtgtcg cgatcgacca tcattcccat cctgcagccg atggtcgcta
     3901 tcaccagcgc gaacatcggc gccttggcca gtccgacgta gacatgcttg atggccaggt
     3961 tgtcctggag tcgggcgagg aaggtggtgg gcgtgatgtc gagcatcggg ccggcgatca
     4021 gcatggcgcc gacgaggctc atcaggtctc ctacgaacac gagcagcggc aatgcgatca
     4081 tcagcgcaaa cacgcgcggc atgaccagca cgtcgagtgg ggacagtccg agcgtacgga
     4141 tggcatcggt ctcctcgttc agccgcatcg aaccgagctg ggcggtgaag gcggcgcctg
     4201 agcggccggc aacgatgact gccacgatga tcggggcgaa ctcgcgtgtg gcgccgatac
     4261 cgacgccgtc gaccacgaag atgttcgcac cgtattgctc ggcctggagc ccgagcaggt
     4321 aagcgaccac gacgccgatc agcagagtga cgagcgacac caccgggatc gcgttaaggc
     4381 acacctgctc gagctgtacg gtcagttccc gcgtccttac ctcggtcggg gacagcacgc
     4441 cgcggaacaa gccgtcgaac accatgccca cgtaggtgag gtgtccggcg acaaggtccc
     4501 gtatcgtgag cgtgtgtcgt ccgatctgcg cgagcagtcc gtggcggtgc cgcgggaccg
     4561 gcgcattgat cacagccagt ctctggcgca cctgctcgac gatgcgcccc tcgttgggtg
     4621 caaagcccga cagttcaacc gtggtcgcgt cgattccgga gcgctgcagg tgatcgagca
     4681 gcgccagggc agcggccgtg tcgatttccg tcacggcagt gccgtcgacg gacagtttcg
     4741 gtggcaggct tagcgcgtcc aacgcatcga cgatggtggg cagggcgccc agccgccacg
     4801 cgccgctcag gtgcaggacg gagtccgcgc catcgtcgag gcgcgcaccg ggaggtgtgg
     4861 ctgggtgcgc aggttcggca ggcatgctgt ggccgttgac agggccgcgg caggctagca
     4921 caggcacttg ccgcgcgaac cggccgatgc ggccgcgact cgtctcagcg cggcacgcag
     4981 gagtgcagca acatcagacc aagcagtgcc aggagcagga gcgggactgc actagaccag
     5041 tgccccggac cgtcgctgcg cgtcacgatg gcggggacga cggtcggttc agagtcttcg
     5101 tgcatggctt gttttccggc accgagcagc tgcctgagta ccgcgccgcg cggccgcgca
     5161 gcgtacaaag tgtgcaggca tctgccgtgg tctcaggcag cgagcagctg tctgagtacc
     5221 gcgccgcgcg gcc
//
LOCUS       DATIRT010000337         1702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_544063, whole genome shotgun sequence.
ACCESSION   DATIRT010000337 DATIRT010000000
VERSION     DATIRT010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544063"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VLN25_07720"
     CDS             <1..280
                     /locus_tag="VLN25_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HSQ10488.1"
                     /translation="TGDVLPPPRVEEVRELLLSHLDDHYRFYGAGLGVRTARKHIIWY
                     TRELVGGRDFCDDMNAIDDCDGQHRAVDIFLRRHGEQHARLSYAPVLH"
     gene            372..578
                     /locus_tag="VLN25_07725"
     CDS             372..578
                     /locus_tag="VLN25_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSQ10489.1"
                     /translation="MLRSLEQYFEDLDGARPHALFEMVTKAIERPLLQFAMSRSAGNQ
                     SAAAELLGINRNTLRKKLQQHKLA"
     gene            606..>1702
                     /gene="purH"
                     /locus_tag="VLN25_07730"
     CDS             606..>1702
                     /gene="purH"
                     /locus_tag="VLN25_07730"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HSQ10490.1"
                     /translation="MTKQALISVSDKDGVVDFARALAAMGYRLLSTGGTARLLAEQGL
                     QVTEVAEYTGFPEILDGRVKTLNPRIHAGLLARRPDAAHMQSLAQHAIDPIDILVVNL
                     YPFEKTVARTDCSFDDAIENIDIGGPAMLRAAAKNHESVAAIVDPGDYTVVLDELRTT
                     NEVSATTRLELAKKVFAHTARYDGMIANYLTALDGARERSVFPDVLTRQWVKVQQMRY
                     GENPHQQAAFYRERVVAPGLLAGYAQLQGKELSYNNIADADAAWECVRSFAECACVII
                     KHANPCGVAVGASPTEAYAKAFKTDPTSAFGGIIAFNRPLDAAAAQAIAKQFVEVVIA
                     PAFDPAAREVLSAKQNVRLLTVALGAAHNDF"
BASE COUNT          325 a          555 c          547 g          275 t
ORIGIN      
        1 cacaggggac gtactgccgc caccgcgcgt ggaagaggtc cgcgaactgc tgctttcgca
       61 tctcgacgac cactatcggt tttacggcgc cgggctcggc gtgcgcaccg cacgaaagca
      121 catcatctgg tacacgcgcg aactcgtcgg tggtcgtgac ttctgcgacg acatgaacgc
      181 catcgacgat tgcgatgggc agcatcgcgc cgtcgacatc tttctccgcc gtcacggtga
      241 gcagcacgcg cggctctcct acgcgccggt acttcattga ggaatcaagc gtggcgaaga
      301 catccgtcaa ggctggcacg gccaccgagc gcgccagccg cgcgcgcagc ggcgaaacgc
      361 tcgaacagtg tgtgctgcgc agcctggagc aatacttcga ggacctcgac ggcgcgcgtc
      421 cgcatgccct gttcgaaatg gtgacgaaag cgatcgagcg gccgctgctg caatttgcga
      481 tgtcgcgcag cgccggaaac cagtcggcag cggccgaact gctggggatc aatcgcaata
      541 cgttgcgaaa gaagctgcag cagcacaagc tggcctgaag cggcagcggc ggggaggccg
      601 caaccatgac caagcaggcg ctgatcagcg tctctgacaa ggacggcgtc gtcgacttcg
      661 cgcgcgcact cgccgcaatg ggttatcgcc tcctgtcgac cggcggtact gcgcgtctgc
      721 tcgccgaaca aggactgcag gtgactgaag tcgctgagta cacaggcttc cccgagatcc
      781 tcgatggccg cgtcaagacg ctgaaccctc gcatccatgc aggcctgctc gcgcgccggc
      841 ccgatgccgc gcacatgcag tcgctcgcgc agcatgcgat cgatccgatc gacatcctgg
      901 tcgttaacct gtaccccttc gagaagacgg tggcgcgtac ggactgcagc ttcgacgatg
      961 ccatcgagaa catcgacatc ggcgggccgg ccatgctgcg ggcggcggcg aagaaccacg
     1021 aaagcgtggc cgcgatcgtc gatcccggcg actacaccgt cgtgctggac gaactcagga
     1081 cgacgaatga ggtcagcgcg acgacgcgcc tcgaacttgc gaaaaaggtg ttcgcgcata
     1141 ccgcacgcta cgatgggatg atcgccaact atctgacggc actggacggc gcacgggagc
     1201 gcagcgtgtt tcccgacgtg ctgacgcggc agtgggtcaa ggtgcagcag atgcgctatg
     1261 gcgagaaccc gcatcagcag gccgcgttct accgcgaacg agttgtcgct cccgggctgc
     1321 tcgccggata cgcgcagctc cagggcaagg agctgtcgta caacaacatc gctgatgctg
     1381 acgcggcgtg ggaatgcgtg cgcagcttcg ccgagtgcgc ctgcgtgatc atcaagcacg
     1441 ccaatccctg cggcgtcgcc gtgggagcgt cgcctaccga ggcctacgcc aaggccttca
     1501 agaccgatcc gacctcggcc tttggcggca tcatcgcgtt caaccgtcct ctcgatgcgg
     1561 ccgccgcgca agccattgcc aagcagttcg tcgaggtggt catcgccccg gcgttcgacc
     1621 cggcggcgcg cgaggtgttg tccgccaagc agaacgtccg cctgctgacg gtggcgctgg
     1681 gcgccgcgca caatgatttc ga
//
LOCUS       DATIRT010000338         1754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1135392, whole genome shotgun sequence.
ACCESSION   DATIRT010000338 DATIRT010000000
VERSION     DATIRT010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135392"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VLN25_07735"
     CDS             complement(<1..575)
                     /locus_tag="VLN25_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSQ10491.1"
                     /translation="MNAGAVLTIESLDTVLDTDSGLVHAVDALALTLTRGETFALVGE
                     SGCGKSMTALSILRLLPDNGRVQGGRMDLSGTDLAQLPEARMRDVRGRRVSIIFQEPA
                     TSLNPVLRVGHQIVEVIERHTALRGAAARAKALEWLRRVGLPDPEQRIDSYSFQLSGG
                     QKQRVMIAIALAGEPDVVIADEPTTALDVTI"
     gene            complement(572..>1754)
                     /locus_tag="VLN25_07740"
     CDS             complement(572..>1754)
                     /locus_tag="VLN25_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSQ10492.1"
                     /translation="RTLSPLDVLLRHAVDAREKTYSVPLAYWSFQRETAVIDGKEVRE
                     LPRLAHGGAHLQDPSRDWKPDLLARSLRGLGGGLAAAVMLTLGIAALRSRGTGWRASV
                     RAMLRAETEIPWPAILATATLIAMFIGWLAAVWPVYHPFGTDRTGNDVLFQALKSIRT
                     AVVIGSLSTIATLPLAIALGVLAGYFKGWVDDVIQYFYTVLSAIPPILLVAAFVLLIN
                     VYIDRNAENFATGVERAEFRLFLLCVILGITGWATLCRLLRAETLKISELEYVQAAHA
                     FGVSHWRIMGRHILPNVMHLVLIVAVLDFSALVLYEAVLSYVGVGVDPNSSSFGSMIN
                     LARSEMSRDPVVWWNLATAFTFMLALVLAANLFADAVREAFDPRARAFRPRRIRLKEA
                     T"
BASE COUNT          327 a          578 c          584 g          265 t
ORIGIN      
        1 tggatcgtca cgtcgagcgc cgtggtcggc tcgtcggcaa tgacgacgtc gggctcgcca
       61 gcgagtgcga tcgcaatcat cacgcgctgt ttctgcccgc ccgacagctg gaacgaataa
      121 ctgtcgatgc gctgctcggg atccgggagc ccgacccgcc gcagccactc cagcgccttt
      181 gcgcgcgctg cggccccgcg cagcgccgtg tggcgttcga tcacctcgac gatctggtga
      241 cccacccgga gtaccggatt caggcttgtg gccggctcct ggaagatgat gctgacgcgt
      301 cgaccgcgca cgtcgcgcat gcgggcctcg ggcaactggg cgaggtcggt gccggacaag
      361 tccatccgtc cgccttgtac ccggccgttg tcgggcagca ggcgcaggat cgacagcgca
      421 gtcatcgact tgccgcagcc agactcgccc accagcgcga aggtctcgcc gcgcgtgagc
      481 gtcagtgcca atgcgtcgac ggcgtgtacc aggccgctgt ccgtgtccag cacggtgtcg
      541 agcgactcga ttgtcagcac ggcacccgcg ttcacgtcgc ttccttcagt cgtatgcggc
      601 gcggccggaa agcgcgcgcg cgggggtcga aggcctcgcg caccgcatcg gcgaacaggt
      661 tcgcggccag caccagcgcc agcatgaaag taaacgcggt cgcgaggttc caccagacca
      721 ccgggtcacg cgacatctcg ctgcgcgcca ggttgatcat cgagccgaag ctcgatgaat
      781 tcggatcgac gccaacaccg acataggaca gcaccgcctc gtacagcacc agcgccgaaa
      841 aatcgagcac ggcgacgatc agcaccaggt gcatcacgtt cggcaggatg tggcgcccca
      901 tgatgcgcca gtggctgacg ccgaacgcgt gtgcggcctg cacgtactcc agttcggaaa
      961 tcttcagcgt ctcggcgcgc agcaaccggc acagcgttgc ccagccggta atgccgagga
     1021 tcacgcacag caggaagagg cgaaactcgg cacgctccac gccggtggcg aagttctcgg
     1081 cgttgcgatc gatatagacg ttgatcagca gcacgaaggc cgcgacgagc aggatcggcg
     1141 ggatcgccga cagaacggtg tagaaatact ggatgacatc gtcgacccag cctttgaagt
     1201 agcccgccag cacgccgagc gcgatggcta gtggcagtgt ggcgatggtc gagagcgacc
     1261 cgatcaccac ggccgtccgg atgctcttca aggcctggaa cagcacgtcg ttgccggtgc
     1321 gatcggtgcc gaacgggtga tagacaggcc acaccgcggc cagccagccg atgaacattg
     1381 cgatcagcgt tgccgtggca agaatcgcgg gccagggaat ctcggtttct gcacgcagca
     1441 ttgcgcggac cgaggcacgc cagccggtac cgcggctgcg caaggcggcg atgcccagcg
     1501 tcaacattac ggcggccgcc agcccgccgc cgaggccacg cagcgaacgc gcaagcaggt
     1561 cgggcttcca gtcgcggctg gggtcctgca gatgggctcc tccatgggcc aggcgcggca
     1621 gctcacggac ttccttgccg tcgattacgg cggtttcgcg ctggaaagac cagtaggcga
     1681 gtggcaccga gtaggtcttc tcgcgggcat cgaccgcgtg gcgcaacagc acgtcgagcg
     1741 gtgacagcgt gcgc
//
LOCUS       DATIRT010000339         1997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_741967, whole genome shotgun sequence.
ACCESSION   DATIRT010000339 DATIRT010000000
VERSION     DATIRT010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741967"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="VLN25_07745"
     CDS             <1..1135
                     /locus_tag="VLN25_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HSQ10493.1"
                     /translation="NHTLVLDCTCTNQDGKTVISGEALVLAPTERIERPRSTLPQVRI
                     ALAQGDDLVRILQYVKPLGRIKMAVVHPCDTVSLSAALDACAAGVIEPVLIGPRARIE
                     AVAQKSDLNIDGIAIEDVPHSHAAAARAVELAVRGEVEALMKGSLHTDELMAAVVASP
                     LRTKRRMSHCFLMQTPAYPRPFIITDAAINIAPTLAEKVDIVCNAVELAHAFGIAQPR
                     VAILAAVETVNPSMPATLDAAALCKMADRGQIPGAILDGPLAFDNAVSAAAARTKGIV
                     SEVAGAADILVVPDLESGNMLAKQLMYMGGAASAGIVVGARVPIVLTSRADPREARIA
                     SCAVALVAARRGSHAAAANVGQQIPIGRAAAAAPPQATLELQA"
     gene            1132..>1997
                     /locus_tag="VLN25_07750"
     CDS             1132..>1997
                     /locus_tag="VLN25_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HSQ10494.1"
                     /translation="MSAIRPTPISATLAAPNGHATLDAASEPILVLNCGSSSIKFALF
                     DSNMLPLTRTPLWAGKVDGIGTANPCLVEADAAPAALTLDGEKPYHGALQAVRACVQR
                     RAGGRLPAVVAHRVVHGGSKYTQPVRIDRDVLAELKGLIPLAPLHQPYALQAIEVLLN
                     EAPGLPQVACFDTAFHRTLPLAEQMLPLPNALWERGIRRYGFHGLSYEYMSIALPERH
                     RRHAEGRTIVAHLGSGASLCAMEGLQSVATTMGFSALDGMMMGTRCGAVDHGVLLHLM
                     ATEKLSVEQLTNL"
BASE COUNT          333 a          685 c          658 g          321 t
ORIGIN      
        1 gaatcacacg ctcgtcctcg actgcacctg tacgaaccag gacggcaaga cagtgatctc
       61 gggcgaggca ctggtgcttg cgccgaccga gcgcatcgag cggccgcgca gcacgctgcc
      121 acaggtgcgc attgcactcg cgcagggtga tgacctcgtg cggatactgc agtacgtaaa
      181 gccacttggg cgcatcaaga tggccgtcgt tcacccctgc gacacggtca gcctctccgc
      241 cgccctcgac gcgtgtgccg ctggagtgat cgagccggtg ctgatcggcc cgcgcgctcg
      301 catcgaggcc gttgcacaga agtcggatct gaacatcgac ggcatcgcga tcgaggacgt
      361 gccgcacagc catgccgcgg cggcacgtgc cgtcgagctc gccgtccggg gcgaggtcga
      421 agcgctgatg aaggggagcc tgcacacgga cgagctgatg gccgccgttg tcgccagccc
      481 gcttcgtacg aaacgccgca tgagccattg cttcctgatg cagacgccgg cgtacccgcg
      541 tccgttcatc atcaccgacg cggcaatcaa cattgcgcca acgcttgccg agaaagtgga
      601 catcgtctgc aacgcggtgg agctggcgca cgccttcggc attgcgcagc cgcgcgttgc
      661 gatccttgca gccgtcgaga cggtcaaccc cagcatgccc gcgacgctcg atgcggcggc
      721 gctgtgcaag atggccgacc gtggtcagat tcccggcgcg atcctcgatg ggcccctcgc
      781 cttcgacaac gcggtgtcgg cggccgccgc ccgcaccaag gggatcgtct cggaggtcgc
      841 cggtgccgct gacatcctgg tcgtccccga cctggaaagc ggcaacatgc tcgccaagca
      901 gctgatgtac atgggcggcg cggcgagcgc cggcattgtc gtcggcgcgc gcgttccgat
      961 cgtgctgacc agccgcgccg atccgcgtga ggcgcgcatc gcctcgtgcg cggtggcgct
     1021 ggtggccgcg cggcgcggct cccatgccgc tgctgccaat gtagggcagc agattcctat
     1081 cggccgggcg gcggccgccg ctccccctca ggcaacgctg gagcttcagg catgagtgcc
     1141 atccggccga cccccatctc agccaccctt gctgccccca acggccatgc gacgttggat
     1201 gcagcaagcg aaccgatcct ggttctgaac tgcggttcct ccagcatcaa gttcgccctg
     1261 ttcgattcga acatgctgcc gctgacgcgc acgccgctgt gggccggcaa agtcgacggc
     1321 atcggcaccg caaacccgtg cttggtcgaa gcagacgccg caccggcggc gcttaccctg
     1381 gacggcgaga agccgtatca cggcgccttg caggcggtgc gcgcgtgtgt gcaacgacgt
     1441 gccggcggac gattgcccgc cgtggtggcg caccgggtgg tgcacggcgg cagcaagtac
     1501 acccagccgg tccgcatcga tcgcgacgta ctggccgagc tcaaaggcct catcccgctg
     1561 gcgccgctgc accagcccta cgcgctgcaa gcgatcgagg ttctgctgaa cgaagcgcca
     1621 gggttgccgc aagtcgcgtg cttcgatacc gcattccacc gtaccctgcc cctggcggag
     1681 caaatgctgc cattgccgaa tgcgctgtgg gagcgcggca tccggcgcta cgggttccat
     1741 gggctgtctt acgaatacat gtcgatcgcc ctgcccgaac gccatcgcag gcacgcggag
     1801 ggacgcacga tcgtcgccca tcttggcagc ggcgccagcc tgtgcgcgat ggaaggcctg
     1861 caaagcgtgg ccacgacgat gggcttttca gcgctcgacg gtatgatgat ggggacgcgg
     1921 tgcggggcgg tcgaccacgg cgtcctgctg cacttgatgg caaccgagaa gctcagcgtg
     1981 gagcagctca ccaatct
//
LOCUS       DATIRT010000340         2587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1528514, whole genome shotgun sequence.
ACCESSION   DATIRT010000340 DATIRT010000000
VERSION     DATIRT010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1528514"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VLN25_07755"
     CDS             <1..285
                     /locus_tag="VLN25_07755"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HSQ10495.1"
                     /translation="LLVESGDWRSRVQRVLVVDCVEAAQISRVAARPGMDEATARAIL
                     HAQATRQSRLAIADDVLYNEAPRVDIESRVALLHWHYVEWARVSALRNSL"
     gene            335..1090
                     /gene="zapD"
                     /locus_tag="VLN25_07760"
     CDS             335..1090
                     /gene="zapD"
                     /locus_tag="VLN25_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HSQ10496.1"
                     /translation="MVLYEYPFSERIRTLLRLEDLFDKLLYFCGEQHAYCHHTALLTL
                     FEILEVTSRADLKSDLLQELERHKQTLNALRNNPQVSEAALATVLSDIDQAQQALNAT
                     TGKAGQHIRDNEWLMSIRSRAIIPGGTCEFDLPSYHAWLTRDLDSRTRDLQAWVQPMM
                     QFQSALGIVLKLLRETAHRSRQTAAQGSFQQMLQGRTYALLQVRVDGSLNAIPEISAN
                     KYMLWIRFMTQDRDLKPRSVERDIPFELALCAF"
     gene            1097..1288
                     /locus_tag="VLN25_07765"
     CDS             1097..1288
                     /locus_tag="VLN25_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HSQ10497.1"
                     /translation="MTTVKCPTCEARTLWNGDNTFRPFCSERCKLIDLGAWASERYVV
                     AGRPSDVAGDAAPDVTAND"
     gene            complement(1285..2232)
                     /locus_tag="VLN25_07770"
     CDS             complement(1285..2232)
                     /locus_tag="VLN25_07770"
                     /inference="COORDINATES: protein motif:HMM:NF006530.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HSQ10498.1"
                     /translation="MTSRPVVDVAVGVLIRPDGRFLLASRPAGKPYAHYWEFPGGKLE
                     PGETVTHALARELHEELGIDIGLAVPWVVREFEYPHAHVRLHFCRVFGWSGDLHAREG
                     QSFRFCAHDDLPDGPLLPATVPVLRWLALPPVYAISNAHELGAALFLQRLDFALERGL
                     RFMAFREPHLDPMDAEPLFRETLQRVRAVGGLLVVSSRHPHPWSLAADGIHLTSSNLM
                     AANQRPECDWVGASVHSEAELAQAAAMGVDFAVLGPVAPTASHPGSLGIGWQSLARWV
                     TAAAVPVYAIGGLNAGDLHRARDAGAQGIALQRAAWPER"
     gene            complement(2229..>2587)
                     /locus_tag="VLN25_07775"
     CDS             complement(2229..>2587)
                     /locus_tag="VLN25_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF815 domain-containing protein"
                     /protein_id="HSQ10499.1"
                     /translation="YATSNRRHLMPEFMRDNLAAQHQEDGEVHPAEAVEERVSLSERF
                     GLWLTFYPFRQDDYLDIAQHWLKQLGVAAVDTERASNEALQFALQRGSRSGRVALQFA
                     RDFAGRHLLEKGRRSR"
BASE COUNT          484 a          847 c          811 g          445 t
ORIGIN      
        1 ctgctcgttg aatcaggtga ctggaggtca cgtgtgcagc gggtcctcgt cgtcgactgt
       61 gtcgaagccg cacagatttc tcgtgtagct gcgaggccgg gaatggatga ggcgaccgca
      121 cgcgcgattc ttcacgcgca ggcgactcgg caatcgcggc tggcgatcgc ggacgatgtg
      181 ctctacaacg aggcgcccag ggtcgacatc gagtcgcgcg tggccctgct gcactggcac
      241 tacgtcgaat gggccagggt ctccgctctg cgcaacagcc tgtgatatcg tcgtccgcat
      301 ccccgcgctt acggcgtcca tgtcgatcga cagtctggtt ctgtacgagt atccgttcag
      361 cgaacggata cgcacactgc tgcggctcga agacctcttc gacaagttgc tgtacttctg
      421 cggcgagcag cacgcctact gccatcacac ggcgctgctg acactgttcg aaatcctcga
      481 ggtcacgtcg cgtgccgacc tgaagtccga cctgctgcag gaactcgaac gtcacaagca
      541 gaccctgaac gcgctgcgca acaatccgca agtttccgag gccgcgctcg ccaccgtact
      601 gtccgacatt gatcaggcgc agcaggccct caatgccacg accggcaagg ccggtcagca
      661 catccgagac aacgaatggc tgatgagtat ccgcagccgc gccatcattc ccggaggcac
      721 ctgcgagttc gacctgccgt catatcacgc ctggctgact cgcgatctcg acagccgtac
      781 gcgcgacctg caggcctggg tgcagccgat gatgcagttt cagtccgcac tcgggatcgt
      841 gctcaagctg ctgcgcgaga cggcgcatcg cagccgtcag accgcggctc agggctcgtt
      901 ccagcaaatg ctccagggcc ggacctatgc gctcctgcag gtgcgggtcg acggatcgct
      961 gaacgcgatt ccggagatca gtgccaacaa gtacatgctg tggatacggt tcatgaccca
     1021 ggatcgcgac ctcaagccac gttcggtcga gcgcgacatc ccgttcgaac tggccctctg
     1081 cgccttctga ttgccgatga cgacggtgaa gtgccccacc tgcgaggcac gcacgctgtg
     1141 gaacggggac aacactttcc ggccgttctg ctcggagcgc tgcaagctga tcgacctggg
     1201 ggcgtgggcc tcggagcggt atgttgtcgc tggccggccg agcgacgtgg ccggcgacgc
     1261 ggcccctgac gtgactgcaa acgactagcg ttccggccag gctgcccgct ggagcgcaat
     1321 gccctgtgcg cccgcgtccc gagcgcgatg cagatctccg gcgttaagtc cgccgatcgc
     1381 atagacggga acggctgcgg ctgtaaccca tcgagccagg ctttgccagc cgattcccag
     1441 tgatccggga tgtgatgccg taggagcgac tggacccagc acggcgaagt cgactcccat
     1501 cgcagccgct tgggcaagtt cggcttcgct atggaccgat gcgccgaccc agtcgcattc
     1561 gggccgttgg tttgcagcca tcaggttgct tgatgtgagg tgtatcccat cggccgctag
     1621 cgaccagggg tgggggtgcc gactgctgac cacgagcaag ccgccaacgg cgcgtacacg
     1681 ttgcagcgtc tccctgaaga gcggttctgc gtccatcggg tccaggtggg gttcgcgaaa
     1741 cgccatgaac ctcagcccgc gttcgagcgc gaaatccagg cgctgtagaa acagcgctgc
     1801 accgagttcg tgtgcattgg agatcgcata gacgggcggc agcgcgagcc agcgcaacac
     1861 cggcaccgtt gccggcaaca agggaccgtc cggcaggtcg tcatgggcgc agaaccggaa
     1921 cgactgcccc tcgcgcgcat gcaaatcgcc cgaccaaccg aagacccggc agaagtgcag
     1981 ccgcacatgg gcgtgcgggt actcgaactc ccgcaccacc cacggaacgg cgagaccgat
     2041 gtcaatgccc agttcctcgt gcaactcgcg cgccagcgca tgcgtgacgg tctctccagg
     2101 ctcgagcttt cctcccggaa actcccagta gtgggcgtag ggctttcccg cgggtcgact
     2161 cgccagcaga aaccggccgt cggggcggat cagcacgccg accgccacgt cgaccaccgg
     2221 acgggaagtc atcgactcct gcggcccttc tcgagcagat gtcggcccgc gaagtcgcgc
     2281 gcaaactgaa gcgcgacgcg cccggaacgc gatccgcgct ggagtgcgaa ctgcagcgcc
     2341 tcgttacttg cacgttcagt gtcgaccgcc gctacgccga gctgcttgag ccaatgctgt
     2401 gcgatgtcga ggtaatcgtc ctggcgaaac ggatagaagg tcagccagag gccgaagcgc
     2461 tcggacaacg acacacgctc ctcgacggct tccgccggat gtacttcacc gtcctcctga
     2521 tgctgcgcgg caaggttgtc gcgcatgaac tccggcataa ggtgacgccg attcgatgtc
     2581 gcataga
//
LOCUS       DATIRT010000341         1675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_151081, whole genome shotgun sequence.
ACCESSION   DATIRT010000341 DATIRT010000000
VERSION     DATIRT010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151081"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..1125)
                     /locus_tag="VLN25_07780"
     CDS             complement(157..1125)
                     /locus_tag="VLN25_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HSQ10500.1"
                     /translation="MNTSRYAIRKRVMVTGGAGFLGSHLCDRLVADGHDVLCIDNFFT
                     GTKDNIAHLIGSPQFELMRHDVTFPLYVEVDEIYNLACPASPIHYQFDPVQTTKTSVH
                     GAINMLGLAKRTKARIFQASTSEVYGDPEVHPQTEAYWGRVNPIGRRSCYDEGKRCAE
                     TLFFDYHRQHNLRIKVARIFNTYGPRMHPNDGRVVSNFIVQALRNDPITIYGDGSQTR
                     SFCYVDDLIEAFIRLMATPDELTGPVNIGNPTEFTIKELAEKVIEMTNSRSKLTFEKL
                     PSDDPTQRRPDISMARQTLGWEPSIQLEAGLRSTIAYFETMLQQGG"
     gene            complement(1122..>1675)
                     /locus_tag="VLN25_07785"
     CDS             complement(1122..>1675)
                     /locus_tag="VLN25_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSQ10501.1"
                     /translation="VLSATHRKLDELVKAAKFREDLYYRINVIELRVPALRERLDDVP
                     PLVDMLLDRVAGQMDVARPQVTDEALDKLLSYSYPGNVRELENILERAVTLCTDDRIA
                     PEDIQLKPGQLLSDLPELAGREFLAGGDAAADGLEGQLEHIQREAIVRALEQTRYNKT
                     KAAELLGMTFRQLRYRVKKLGIE"
BASE COUNT          278 a          533 c          524 g          340 t
ORIGIN      
        1 tgggacgagg cgcagctcgt ggccatgatc gagcgcgagc tggcgttgcc gcccgggtcg
       61 aggccggtgc gccgccgcgg cactgagctg gtcgactttc tgcgcgactg gctgcgcacg
      121 accctcactc gctgacgcag gccgctgtcg gcggcgtcag ccgccctgtt gcagcatcgt
      181 ttcgaagtac gcgatcgtgg agcgcagccc ggcctcgagt tgaatgctgg gctcccaacc
      241 cagcgtctgc cgcgccatcg agatgtctgg ccggcgctgt gtcgggtcgt cactcggcag
      301 cttctcgaag gtcaacttcg accgggagtt ggtcatttca atgacttttt ccgccagttc
      361 cttgatggtg aattcggtgg gattgccgat gttcacaggg ccggtcaatt cgtcgggcgt
      421 ggccatcagg cggatgaagg cctcgatcag gtcgtcgaca tagcagaacg accgcgtctg
      481 cgagccatcc ccgtaaatcg tgatgggatc gtttctcagc gcctgcacga tgaagttcga
      541 tacgacgcgc ccgtcgttgg ggtgcatgcg gggcccgtag gtgttgaaaa tccgtgcgac
      601 cttgatgcgc aggttgtgct ggcggtggta gtcgaaaaac agcgtctctg cgcagcgctt
      661 gccttcgtcg taacacgaac gacggccgat cgggttgacg cgtccccagt aggcctcggt
      721 ctgcgggtga acttcggggt cgccatacac ctcgctcgtg gatgcctgga aaatcctggc
      781 cttggtccgc ttggccaacc cgagcatgtt gattgcgccg tgcactgagg tcttggtggt
      841 ctgcaccgga tcgaactggt agtggatcgg tgacgcggga cacgcgaggt tgtaaatctc
      901 gtcgacctcc acgtagagcg gaaacgtcac gtcgtgccgc atcagctcga actgcgggct
      961 gccgatcaaa tgcgcgatgt tgtccttggt gcccgtaaag aagttgtcga tgcagagcac
     1021 gtcgtggccg tccgccacga ggcgatcaca aaggtgcgag ccaagaaacc ccgcgccacc
     1081 ggtaaccatg acccgcttgc ggatcgcata tcgactcgtg ttcattcgat ccccagcttc
     1141 tttacccgat accgcaactg cctgaacgtc attcccagca actccgccgc cttcgtcttg
     1201 ttgtaccggg tctgctccag cgcccgcacg atggcctcgc gctggatgtg ctcgagctgt
     1261 ccctccagtc catcggcagc tgcatcccct ccggcgagga attcgcgccc cgccagctcc
     1321 ggcaggtccg acagcagctg ccccggcttc aactggatat cctccggcgc aatccggtca
     1381 tccgtgcaca gcgtcaccgc gcgctccagg atgttttcca gttcgcgcac gttgcccggg
     1441 taggagtacg agagcagctt gtccagggcc tcgtcggtga cctggggccg cgccacgtcc
     1501 atctggccgg ccacgcgatc cagcagcatg tccaccagcg gtggcacgtc atccaggcgc
     1561 tcgcgcagcg ccggcacgcg cagctcgatc acgttgatgc ggtagtacaa gtcctcgcgg
     1621 aacttcgcgg ctttcacgag ttcatccaat ttgcggtgcg tggcggagag cacac
//
LOCUS       DATIRT010000342         1806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1626750, whole genome shotgun sequence.
ACCESSION   DATIRT010000342 DATIRT010000000
VERSION     DATIRT010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1626750"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..1262)
                     /locus_tag="VLN25_07790"
     CDS             complement(168..1262)
                     /locus_tag="VLN25_07790"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HSQ10502.1"
                     /translation="MAGRQVSLRSELQPLKSIYDAGKAAVLANVGTLVVPTTMAQYRA
                     RSVPLPPSLFSHNDQQSVWQTYNGRREGARVGWGGRMGDLLATANQFQNFTCVSASGT
                     AVFLSGQTSVQMQIGNSGGLAITGVSGNTLYGSTAGPATARRLMTEDRANLFEKDYNT
                     IVKRSTEAAQTLNTALAGFPTTAAPFSTFPNTGLGNQMRNVARMIAARQALGATRQVF
                     HVSIGGFDQHSGLINGHGGRMTELANAMAAFYQATVSLGVADSVTAFTASDFGRTLDS
                     NGEGSDHGWGSHHFVVGGAVKGGELYGTWPETVLRGPNDVGRGNLLPTTSVNQYAATL
                     ATWFGIADAQMADVLPNAANFPQGNLGFMV"
     gene            complement(1302..1556)
                     /locus_tag="VLN25_07795"
     CDS             complement(1302..1556)
                     /locus_tag="VLN25_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10503.1"
                     /translation="MTTQASRREFLRTASRLSVAGVATPWAVNLAAIGAAAAQTAPTD
                     YKALVCVFMYGGNDHSNMVVPFDNASYTLYAQSRTNLTLA"
     gene            complement(1573..>1806)
                     /locus_tag="VLN25_07800"
     CDS             complement(1573..>1806)
                     /locus_tag="VLN25_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HSQ10504.1"
                     /translation="SDVRADYTSTYALADNADALVARINLMLAASSLSTATVTSIRDA
                     VNAIPMTAGNARQNRTQLAIYLTMTSPEFLVQK"
BASE COUNT          311 a          566 c          594 g          335 t
ORIGIN      
        1 gctgaagaaa ccgctgatgc gcggcatcgg ctcataccgc cgcgtcggtc tcggactgct
       61 gcaactgtcg agctaggcag ccggagctcc ctgaagtcgg gaggggaacg aactgcaaac
      121 gaaaacggcg cccgcgggcg ccgtttcgtt tcgatgatcg caaaaaacta gaccatgaaa
      181 ccgaggtttc cctgcgggaa gttcgccgca ttcggcagca catcggccat ctgggcatcg
      241 gcaataccga accatgtcgc cagcgtcgcc gcgtactgat tgaccgaggt cgtcggcaac
      301 aggttgcctc gaccgacatc gttggggcca cgcagcacgg tctcgggcca ggtgccgtac
      361 aactcgccgc ccttgacggc accaccgact acgaagtggt gcgaacccca gccatggtcc
      421 gaaccttcgc cgttggagtc gagcgtacgg ccgaagtccg aggcagtgaa cgccgtcacg
      481 ctgtcagcga ctccgaggct gacggtggcc tgatagaacg ccgccatcgc gttggccagt
      541 tcggtcatgc ggccgccatg tccgttgatc agtcccgagt gctggtcgaa gccgccgatc
      601 gatacatgaa agacctgtcg cgtggccccg agtgcctgtc gcgccgcgat catgcgtgcg
      661 acattgcgca tctggttgcc gagacccgtg ttcggaaacg tcgaaaatgg cgccgcggtc
      721 gtcgggaacc cggcaagcgc cgtgttcagt gtctgggccg cctcggtcga ccgcttgacg
      781 atcgtgttgt agtccttctc gaagagattg gcgcgatcct cggtcatcaa ccgccgcgcg
      841 gtcgcgggtc cggcggtcga cccgtacagc gtgttgcccg agacaccggt gatcgccagg
      901 ccgccgctgt tgccgatctg catctgcacg ctggtctgtc ctgagaggaa gaccgcggtt
      961 cccgatgccg acacgcaggt gaaattctgg aactggttcg ccgtcgccag caggtcgccc
     1021 attcggccgc cccagccaac acgggcgcct tcgcggcggc cgttgtaggt ctgccagacc
     1081 gactgctggt cgttgtgcga gaaaaggctc ggcggcagcg gcacactgcg cgcccgatac
     1141 tgggccatcg tcgtcggtac gaccaacgtg ccgacgttgg ccaggaccgc ggccttgccg
     1201 gcgtcataga tcgacttcag cggctgcagt tcggaccgca gcgagacctg gcgccctgcc
     1261 atcgacgggt tggtcggcgt ggcaagcggt agcagcgatg tttacgccag tgtcaggttg
     1321 gtacgcgact gcgcatacag cgtgtagctg gcattgtcga acggcaccac catgttcgag
     1381 tgatcgttgc cgccgtacat gaagacgcag accagcgcct tgtagtcggt cggtgccgtc
     1441 tgcgctgctg cagcaccgat cgcggcgaga ttgaccgccc agggcgtggc cacgccggcc
     1501 accgacaatc gtgatgccgt gcgcaggaat tcgcggcggg aagcttgggt cgtcatcggt
     1561 ttgtccgtcc gcttatttct gcaccaggaa ttccggtgac gtcatcgtca ggtagatcgc
     1621 gagctgcgtc cggttctggc gggcgttgcc ggccgtcata gggatcgcgt tgaccgcgtc
     1681 gcggatggaa gtgacggtcg cggtcgagag tgaactcgcc gccagcatga ggttgattcg
     1741 cgccacgagc gcatcggcgt tgtcagccag tgcataggtc gacgtgtagt ccgctctcac
     1801 gtcact
//
LOCUS       DATIRT010000343         1628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1135980, whole genome shotgun sequence.
ACCESSION   DATIRT010000343 DATIRT010000000
VERSION     DATIRT010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135980"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="VLN25_07805"
     CDS             <1..1053
                     /locus_tag="VLN25_07805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSQ10505.1"
                     /translation="ATVLTLAGVTKHYGEVPALRGVDLEVHSGELLTLLGPSGSGKTT
                     LLKVIAGFEIAEGGVVSLVGRDITTLAPNKRDIGMVFQNYALFPHMSVAANIAFPLNI
                     RKVPKQAIARQVADALQLVGLAGYGERLPKQLSGGQQQRVALARAVVFQPRLLLLDEP
                     FGALDRKLREQMQLEVRRLQQRLGITTIFVTHDQEEALVLSDRIAVMSDGVIEQLGDP
                     RAIYASPVNRFVADFIGESNLLRARIDATGEAALLDSGASVGLPQRGNANAEVGLLIR
                     PEAIELARASTDAEGFAGTVDEVVYLGSTLKYFVRLDSGAVIIARRSFGQLPVFAAGD
                     SVVASWSPADVHVVQW"
     gene            1050..>1628
                     /locus_tag="VLN25_07810"
     CDS             1050..>1628
                     /locus_tag="VLN25_07810"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSQ10506.1"
                     /translation="MSVLRASRVDREPLLHAALPLAFLGVLFLYPVARFLLISIDGGS
                     LAQYDKALFDGLYLRVLADTFRIALIVTLITLILGYPIAYFLSSAPPRWATVGLVFLI
                     LPFWTSVLIRTYAWSVILGRNGVVNRLLLSSGVVDEPLRLLNNEVGVLIGMVHVLLPY
                     MVFPLYAVMKRQDPTLLLASQGLGASGPQTFWR"
BASE COUNT          252 a          504 c          538 g          334 t
ORIGIN      
        1 gccactgttc ttacccttgc gggagtgacc aagcactacg gtgaggtccc ggccctgcgc
       61 ggcgtcgatc tcgaggtcca ctcgggcgaa ctgctgaccc tgctcggacc cagcggttcg
      121 ggcaagacga cgttgctgaa agtcattgcc gggttcgaga ttgccgaagg tggagtggta
      181 tcgctcgtcg ggcgcgacat cacgacgctc gcgcctaaca agcgcgacat cggcatggtg
      241 ttccagaact acgccttgtt tccgcacatg agcgtggcgg cgaacattgc gtttccgttg
      301 aacatccgca aggtgccgaa gcaggccatc gcgcgacagg tggcagacgc gctgcagctg
      361 gtggggctgg cgggctacgg cgagcgcctg ccgaagcaac tttcgggtgg acagcagcag
      421 cgggtcgcac tggcgcgtgc ggttgtcttc cagccgcgcc tgctgttgct ggacgaaccg
      481 ttcggagcgc ttgatcgcaa gctgcgggaa cagatgcagc tcgaggtgcg tcggttgcaa
      541 cagcgcctgg gcatcacgac catcttcgtc acccacgacc aggaggaggc actcgtcctg
      601 tcggaccgca tcgccgtgat gagcgacggt gtgatcgaac aactcggcga tccgcgagca
      661 atctatgcaa gtcccgtgaa ccgcttcgtc gctgacttca tcggcgaatc caatttgctg
      721 cgcgcccgca tcgatgcgac cggcgaggcg gcgttgctcg acagcggcgc gtccgtgggc
      781 ctgccccagc ggggaaacgc gaacgccgaa gtcggcctgc tgatccggcc ggaggccatc
      841 gaactggcac gcgcatcgac agatgcggag ggattcgcgg ggacggtcga cgaagtcgtc
      901 tacctcggta gcaccctgaa gtacttcgtg cggctcgaca gtggtgccgt catcattgcg
      961 cgacgttcgt tcggacagct gccggtcttt gctgcaggag acagcgtcgt tgcgtcctgg
     1021 tcgccggccg atgtgcacgt ggtgcagtgg tgagcgtcct gcgcgcttcc cgcgttgacc
     1081 gggagccgct gctgcatgcg gcgctgccgc tcgcgttcct tggagtgctc tttctctatc
     1141 ccgttgcccg tttcctgctg atcagtatcg acggcggttc gctggcgcaa tacgacaagg
     1201 cgctgttcga tggcctgtac ctgcgggtcc tggcagacac cttccggatt gcattgatcg
     1261 tcacgctgat tacgttgatc ctcggatatc cgatcgctta cttcctgtcg tcggcgccgc
     1321 cgcgctgggc caccgtcggt ctggtcttcc tgatcctgcc cttctggacc agcgtgctga
     1381 tccggaccta cgcatggtcg gtgatcctgg ggcgaaacgg cgtcgtgaac cgcctgctgc
     1441 tgtcctccgg cgttgtcgac gagccattgc ggctgctgaa caacgaggtc ggagtgctga
     1501 tcgggatggt gcatgtcctg ctgccctaca tggtgtttcc actctatgcg gtgatgaagc
     1561 ggcaggatcc gacgctcctg cttgcgtcac agggtctcgg cgcctcgggg ccgcagacgt
     1621 tctggcgc
//
LOCUS       DATIRT010000344         4135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_250148, whole genome shotgun sequence.
ACCESSION   DATIRT010000344 DATIRT010000000
VERSION     DATIRT010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250148"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VLN25_07815"
     CDS             <1..216
                     /locus_tag="VLN25_07815"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HSQ10507.1"
                     /translation="DFSDNPFAQCRTHLLLITEKDAVKCERVDALRKDPRIWVVPLVA
                     TIDPALVDLVVTALNHLRKGPHGSPAS"
     gene            197..379
                     /locus_tag="VLN25_07820"
     CDS             197..379
                     /locus_tag="VLN25_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HSQ10508.1"
                     /translation="MDHRLLDILVCPVCKGPLDFQKDAQELVCKADALAYPIRDGIPV
                     MLESEARKVALAELGR"
     gene            407..1171
                     /gene="kdsB"
                     /locus_tag="VLN25_07825"
     CDS             407..1171
                     /gene="kdsB"
                     /locus_tag="VLN25_07825"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016473756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HSQ10509.1"
                     /translation="MSFVVIIPARLASSRLPRKPLADIGGAPMVVRVAQRAQATRADR
                     VVVAADSEEIVAACQAHGIDAVLTSPQHETGTDRLAEAARLLGLGKEQIVVNVQGDEP
                     LIPAAVINDVAHLLDRNPDCQIATAAHAISDAEEFFNPNVVKVVTDRKNRALYFSRAP
                     IPWARDAFAASRDRLPPAYPARRHVGLYAYRVEFLEQFPTLPPDALEVAECLEQLRAL
                     AHGFRISVLGLEAALPPGVDTPADLEHVRALYAAQR"
     gene            1206..2768
                     /locus_tag="VLN25_07830"
     CDS             1206..2768
                     /locus_tag="VLN25_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSQ10510.1"
                     /translation="MRMTTVALLAVLAVAGAQAKTLRWASQGDYQTADPYSQNELLTN
                     LFAQQVHDTLVMRDKDMKIVPALAVSWTQVNPTTWRFNLRKGVKFHDGAPFTADDVVF
                     SFERASHTNSQIRQYAIPSGKPRKVDEFTVEFVQDKPNPITLEHIATIFIMNKAWAEK
                     NKVERPLSFKDREETYAARNANGTGPFMLVSREPGVKSVLKKNPAWWGTVTGNVDEIV
                     YSPIGSDATRIAALLSGQVDFILDPPPQDIARLKATKDIKVVEGQENRIIFFGFDQSR
                     DELLYSNVKGKNPFKDKRVRQAVYQAIDIEALKGKTMRGAALPTGGITPSTLPSTPEI
                     EKRRPHDPEAAKRLLAEAGYPNGFEVTLDCPNNRYINDEEICVAVAAMLSRIGIQTKV
                     TAQPRTTYFPKLEKFDTSFYMLGWGGAITDAQTMLSPVLRSKDDKTGNGSFNYGRYVN
                     PKLDAMIDAAAVETDPDKRKQLVTGALTEHNEQVHHVPLHRQVIPWAMRSNVNVVHRP
                     DNWLIGEWVRID"
     gene            2993..3649
                     /gene="adk"
                     /locus_tag="VLN25_07835"
     CDS             2993..3649
                     /gene="adk"
                     /locus_tag="VLN25_07835"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HSQ10511.1"
                     /translation="MRLILLGPPGAGKGTQAAYIKERFGIPQISTGDMLRAAVKDGTP
                     LGLAAKKVMDAGGLVSDDIIIGLVKHRLKEPDTANGYLFDGFPRTIPQAEAMKHAGVA
                     IDYVLEIAVPDADIIDRMSGRRVHPSSGRTYHVRFNPPKAEGRDDATGESLIQRDDDK
                     EETVRKRLEVYHAQTSVLVDYYSQWAASGAAGAPKYRKVVGVGSVDEIRQRAFDALSS
                     "
     gene            3690..>4135
                     /locus_tag="VLN25_07840"
     CDS             3690..>4135
                     /locus_tag="VLN25_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HSQ10512.1"
                     /translation="MSGLALALLCGLAAVVFGAWSISWILKQPAGNARMQEIAAAVQT
                     GAQAYLNRQYRTIAIVGAVLAVIIAVVPQLGLWTAIGFVLGAVLSGAAGYIGMNVSVR
                     ANVRTAQAATLGLNPALNIAFRGGAITGLLVVGLGLIGVAGFYWIL"
BASE COUNT          795 a         1347 c         1327 g          666 t
ORIGIN      
        1 gacttcagcg acaacccctt tgcacagtgc cggacccatc tgttgctgat caccgaaaag
       61 gacgccgtaa aatgcgaacg ggtcgatgcg ctgcgcaagg atccgcgcat ctgggtcgtg
      121 ccgctggtcg cgacgatcga cccggcgctc gttgatctcg tcgtcaccgc actgaaccat
      181 cttcgaaagg gcccgcatgg atcaccggct tcttgacatc ctcgtatgcc cggtctgcaa
      241 ggggccgctt gattttcaaa aggacgcgca ggaacttgtc tgcaaggccg atgccctggc
      301 ctatcccatc cgcgacggca taccggtgat gctcgagtcc gaggcacgca aggtcgccct
      361 cgccgaactc ggacgctgag cgtctgacag tggcacccgc cgtcctgtga gctttgtcgt
      421 catcatcccg gcgcgactgg cgtctagccg gttgccacgc aagccgcttg ccgacattgg
      481 cggcgccccc atggtggtcc gcgtcgccca gcgcgcgcag gcgacgcgag ccgaccgcgt
      541 cgtggtcgcc gccgacagcg aggaaatcgt cgcggcctgc caggcccacg gcatcgacgc
      601 cgtgcttacg tcgccgcagc atgaaaccgg caccgatcgg ctggccgagg cggcccgcct
      661 gcttggactg ggcaaggagc agatcgtcgt caacgtccag ggcgacgagc cactgatccc
      721 ggcggcggtg atcaatgacg tggctcacct gctcgaccgc aacccggact gccagatcgc
      781 gaccgcggcg catgcgatct ccgatgccga ggaattcttc aacccgaacg tcgtcaaggt
      841 cgtgacagac cggaagaatc gggcgctgta cttctcacgc gcgccgattc cctgggcgcg
      901 cgacgccttc gcggccagcc gggaccggct tccgcccgct tacccggccc gccggcatgt
      961 cggcttgtat gcctacaggg tcgaatttct cgagcagttc ccgacgctgc caccggatgc
     1021 gctggaggtg gcggaatgcc tggaacagct gcgcgcgctg gcccacggat ttcgaatctc
     1081 cgtcctcgga ctcgaagcgg ccctgccgcc gggggtggat accccggccg acctcgaaca
     1141 tgtacgtgcc ctgtacgcgg cgcagcgata gactgcgcgg cctcgacaga aggagccttc
     1201 cgatgatgcg catgaccacc gtggcactgc tggccgtact cgctgtcgcc ggtgcgcaag
     1261 ccaagaccct gcgctgggcc tcgcagggcg actaccagac ggccgacccg tattcgcaga
     1321 acgagttgct gaccaatctc ttcgcgcagc aggtgcatga cacgctggtg atgcgcgaca
     1381 aggacatgaa gatcgttcct gcgctggcgg tgtcgtggac gcaggtcaac ccgaccacgt
     1441 ggcgcttcaa cctgcgcaag ggggtcaagt ttcacgacgg cgcaccgttc accgcggacg
     1501 atgtcgtctt ctcgtttgag cgtgccagtc acacgaattc gcagatccgc cagtacgcca
     1561 ttccgtccgg caagccgcgc aaggtggacg agttcacggt cgagttcgta caggacaagc
     1621 ccaatccgat cacgctcgag cacatcgcca cgatcttcat catgaacaag gcctgggccg
     1681 agaagaacaa ggtcgagcgc ccgctgtcgt tcaaggaccg cgaggaaacc tacgccgcgc
     1741 gcaatgccaa cgggaccggc ccattcatgc tcgtgtcgcg cgaacccggc gtgaagagcg
     1801 tcctgaaaaa gaatccggcg tggtggggca cggtgaccgg caatgtcgac gagatcgtgt
     1861 actcgccgat cggctccgac gcgacgcgca tcgccgcact gttgtcgggc caggtcgact
     1921 tcatcctcga cccgccgccg caggacatcg cgcgcctgaa ggccaccaag gacatcaagg
     1981 tggtcgaagg ccaggaaaac cggatcatct tcttcggctt tgaccagagc cgcgacgaac
     2041 tcctctactc gaacgtcaag ggcaagaatc cgttcaagga caagcgtgta cggcaggcgg
     2101 tctaccaggc catcgacatc gaggcactga agggcaagac catgcgcggc gcggcgttgc
     2161 ccaccggcgg catcacgccg tcgacgctgc cttcgacacc ggaaatcgag aaacgccgtc
     2221 cgcacgatcc ggaggcggcg aagaggctgc tggcagaagc cggctacccg aacggcttcg
     2281 aggtgacgct cgactgcccg aacaaccgtt atatcaacga cgaggaaatc tgtgtcgcgg
     2341 tggcggccat gctgtcgcgc atcggcatcc agaccaaggt caccgcgcaa ccgcgcacca
     2401 cctacttccc gaagctcgag aagttcgaca ccagcttcta catgctcggc tggggcggcg
     2461 cgatcaccga cgcgcagacg atgctgtcac cggtgctgcg ctcgaaggac gacaagaccg
     2521 gcaacggcag cttcaactac ggccgctacg tcaacccgaa gctcgatgcg atgatcgatg
     2581 cggccgcggt cgagaccgat ccggacaagc gcaagcaact cgtcacaggg gcgctcaccg
     2641 aacacaacga gcaggtgcac cacgttccac tgcatcggca ggtgattccc tgggcgatgc
     2701 gcagcaacgt caacgtcgtg catcggccgg acaactggct gatcggcgag tgggtcagga
     2761 tcgactgatt gcgctgcttc gcgcggaagg ggccgccttg ccggcccctt ctgctttttt
     2821 ggggccgcga aggaaagtcg gcgaccgagg ccgcgggagg ccctgggctc gtcggcgcag
     2881 ggaacgaacc gggtagaatc ccgcggcttc agcgcctgcg acagaccggt agcagccagt
     2941 agtcgactgc gacagcagaa caacgaccac gagtcgagcg aaggacaggg ggatgcgact
     3001 gatattgctc gggccgccgg gcgccggcaa aggcacgcag gccgcgtaca tcaaggagcg
     3061 cttcggcatc ccgcagattt caaccggaga catgctgcgc gcggccgtca aggacggcac
     3121 tccgctgggc ctggccgcca agaaagtgat ggatgcaggc gggctggtgt ccgacgacat
     3181 catcatcgga ctggtcaagc accggctcaa ggaacccgat accgcgaacg ggtacctgtt
     3241 cgacggattc ccgcgcacga tcccgcaggc cgaggcgatg aagcatgccg gcgtcgcgat
     3301 cgattacgtg ctcgaaatcg cggtcccgga cgcggacatc atcgaccgca tgagcgggcg
     3361 gcgagtgcac ccttcgagtg gccgcaccta tcacgtccgt ttcaatccgc cgaaggccga
     3421 aggtcgcgat gacgcaaccg gcgagtcgct gatccagcgc gacgatgaca aggaagagac
     3481 agtccgcaaa cgtcttgagg tctaccacgc gcagacgagc gtgctcgtcg actattactc
     3541 ccagtgggcg gccagcggcg ccgcgggtgc accgaagtac cggaaggtcg tcggcgtcgg
     3601 gtcggtggac gaaatcaggc agcgcgcgtt tgacgcgctg tcgtcgtgat tcagtgaact
     3661 gatacagaag tttcgacaag gagaagaaga tgtctggact ggcactcgcg ctgctctgcg
     3721 gtctcgcggc cgtggtattc ggcgcgtggt cgatttcatg gatcctgaag cagcccgcgg
     3781 gcaacgcgcg catgcaggaa attgccgctg ccgtgcaaac cggcgcacag gcctacctca
     3841 accggcagta ccgcacgatc gcgatcgtcg gtgccgtgct cgccgtcatc attgcggtgg
     3901 tcccgcagct cggcctgtgg accgcgatcg gattcgtgct cggtgctgtg ctctcgggcg
     3961 cggccggcta catcggcatg aatgtgtcgg tgcgcgccaa cgtgcgcacc gcccaggccg
     4021 cgaccctggg actgaacccg gcgctcaaca tcgcgtttcg cggcggtgcg atcaccggcc
     4081 tgctggtggt cggactggga ctgatcggcg tggcgggctt ctactggatc ctgaa
//
LOCUS       DATIRT010000345         2014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1136086, whole genome shotgun sequence.
ACCESSION   DATIRT010000345 DATIRT010000000
VERSION     DATIRT010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136086"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /gene="rimO"
                     /locus_tag="VLN25_07845"
     CDS             complement(<1..1110)
                     /gene="rimO"
                     /locus_tag="VLN25_07845"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HSQ10513.1"
                     /translation="MGFVSLGCPKALVDSELILTQLRAEGYATSATYEGADLVIVNTC
                     GFIDSAVEESLDAIGEALAENGKVIVTGCLGARNDGGENLVTRVHPKVLAVTGPHAAG
                     EVLDHVHRHLPKPHDPYTDLVPPAGIKLTPRHYAYLKISEGCNHRCSFCIIPSMRGDL
                     VSRPIGQVMREAEALVNQGVREILVISQDTSAYGVDVRYKLDFVGGRTVKTRMTDLVR
                     ELSQLAQAHDAWIRLHYVYPYPHVDEVIPLMAEGKVLPYLDVPFQHSHPRVLKAMKRP
                     ASGEKNIERIRAWRAICPDLTIRSTFIAGFPGESEAEFDHLLDFLREAELDRVGCFAY
                     SPVAGAAANALVGPLPDEVREERRARFMQVQEEISA"
     gene            complement(1202..1741)
                     /gene="phaR"
                     /locus_tag="VLN25_07850"
     CDS             complement(1202..1741)
                     /gene="phaR"
                     /locus_tag="VLN25_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011402311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HSQ10514.1"
                     /translation="MATPVRLIKKYPNRRLYDTQTSTYITLADVKQLVLDSENFQVVD
                     AKSAEDLTRPILLQIILEEEAGGMPMFSAVMLSQIIRFYGNAMQGMMGSYLEKNIQAF
                     IDIQGKLSEQSKAFYETNKIGPEMWTQFVNMQSPMIQSMMSNYIEQSKNLFVQMQENM
                     QNQARSMFTGFPGFPGEKK"
BASE COUNT          335 a          637 c          630 g          412 t
ORIGIN      
        1 ggccgatatc tcttcctgca cctgcatgaa gcgggcacgg cgttcttccc gcacctcgtc
       61 cggcagcggg ccgaccaggg cattggcggc ggcacctgcc accggcgagt aggcaaagca
      121 gccgacccgg tcgagttcag cctctcgcag aaagtcgagc aggtgatcga attccgcttc
      181 gctctcgccc gggaaaccgg cgatgaaagt ggagcggatc gtgaggtccg ggcagatcgc
      241 gcgccaggcg cggatgcgct cgatgttctt ctcgccggac gccggccgct tcatcgcctt
      301 gagcacgcgc ggatgtgagt gctggaacgg cacgtcgagg taaggcagca ccttgccttc
      361 ggccatcagc gggatgacct cgtcgacatg cggatacggg tagacatagt gcagcctgat
      421 ccacgcatcg tgggcctgcg cgagctgtga aagttcgcgt acaaggtcgg tcatcctggt
      481 cttcactgtg cgtccgccga cgaagtcgag cttgtagcgg acatcgacgc cgtaggcgct
      541 ggtgtcctgc gagatgacga gaatctcgcg cacgccctgg ttcaccagcg cctcggcctc
      601 gcgcatgacc tggccgatcg gtcgcgaaac gagatcgcct cgcatcgacg gaatgatgca
      661 gaagctgcag cgatggttgc agccctcgga gatcttgaga tacgcgtagt gccgcggcgt
      721 cagcttgatg cctgccggcg gcaccaggtc ggtgtacggg tcatgcggtt tcggcaggtg
      781 ccggtgcacg tggtcgagca cctcgcccgc ggcatgaggc ccggtgacgg cgagcacctt
      841 cggatgcacc cgggtgacca ggttctcgcc accgtcattg cgcgcaccga ggcagcccgt
      901 gacgatcacc ttgccgtttt cggccagcgc ctcaccgatc gcgtcgagcg attcttccac
      961 ggccgagtca atgaagccgc aggtgttgac gatgacgaga tctgctccct cgtaagtcgc
     1021 gctggtggcg tagccctcgg cgcgcagctg cgtcaggatc agttccgagt cgaccagcgc
     1081 cttggggcag cccagcgaca cgaagcccac cgacggcgcg gtgggctcgg cagtgcgtcg
     1141 ggcagcagtc gggcgcgacc gccccgcgga cctcgtggcg gtcatcgccg ctcgcggctg
     1201 cttatttctt ctcgcccggg aagcccggga agcccgtgaa catcgaacgt gcctggttct
     1261 gcatgttctc ctgcatctgc acgaacaggt tcttcgactg ctcgatgtag ttcgacatca
     1321 tcgactggat catcggcgac tgcatgttga cgaactgtgt ccacatctca ggcccgatct
     1381 tgttcgtctc gtagaaggcc ttgctttgtt ccgacaattt gccctggatg tcgatgaacg
     1441 cctggatgtt cttctcgagg tacgagccca tcatcccctg catcgcgttg ccatagaatc
     1501 ggatgatctg cgacagcatc accgcgctga acatcggcat gccgccagcc tcttcttcca
     1561 ggatgatctg cagcaggatc gggcgcgtca gatcctctgc gctcttcgcg tccacgacct
     1621 ggaagttctc gctgtccagc accagctgct tgacgtccgc cagcgtgatg taggtgctgg
     1681 tctgggtgtc gtacagacgt cggttcggat acttcttgat gagtcgaaca ggtgttgcca
     1741 ttgatgatcc tcgattgtct tcgcgcagtg cgtgcgcgtc ggcccgtcgt tgcgggcatc
     1801 tcccttgcat gtcgcgcgat tctaacgaat ttgccgcagc gcagcggacg acggccccgg
     1861 atcgatatgg gtagcccggg ctcaagcgtc tctgccagaa tcgagcggtt gctctaccca
     1921 ctgctattcg gccgcattca aggagacatg atggttcgcc gcacgttccc cacccggttc
     1981 ttcacccgat cgatcgcagg actcgttctg gcgc
//
LOCUS       DATIRT010000346         6311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_743112, whole genome shotgun sequence.
ACCESSION   DATIRT010000346 DATIRT010000000
VERSION     DATIRT010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743112"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /gene="hpnD"
                     /locus_tag="VLN25_07855"
     CDS             complement(<1..780)
                     /gene="hpnD"
                     /locus_tag="VLN25_07855"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HSQ10515.1"
                     /translation="MNPDAYCQQKAASSGSSFYYSFLFLPPERRRAITAVYAYCREVD
                     DVVDEVSDPTVAAAKLAWWRSEIDALVRGQPSHPVTRALQPFIVGPYGVTQQRLHAVL
                     DGMEMDLRQNRYLDFASLRRYAHLVAGVVGEMSASIFGYRHPATLEYAERLGLALQLI
                     NIVRDVGDDARRGRLYLPLDELQRFGVKAADVLNARYVDGFVPLMRFQAGRARAFYRE
                     ALALLPAEDRKAQRTGLIMGAIYATLLDEIERADFQVLHQRI"
     gene            complement(830..1639)
                     /gene="hpnC"
                     /locus_tag="VLN25_07860"
     CDS             complement(830..1639)
                     /gene="hpnC"
                     /locus_tag="VLN25_07860"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HSQ10516.1"
                     /translation="MGVGHYENFPVASLLAPPALRPAIRAIYRFARTADDIADEGDAV
                     PAERLRELDALVSELDAIERGESGGWPDLAAAVRAHRLPLAHFRDLLSAFAQDVVTLR
                     YATFADVRDYCRRSANPIGRLLLALYETTDAEAMAWSDSICTGLQLVNFWQDVAVDWG
                     KGRVYLPREDMERFDVGEAHIAEQHVDHRWTALMRFEVDRARQLLHGGDPLTRALRGR
                     IGLELRLVVQGGLRILERIDLCDGDVFRRRPLLAARDWLVMGWRSLQGAPT"
     gene            1738..1824
                     /locus_tag="VLN25_07865"
     tRNA            1738..1824
                     /locus_tag="VLN25_07865"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1772..1774,aa:Leu,seq:tag)
     gene            1875..3182
                     /gene="tig"
                     /locus_tag="VLN25_07870"
     CDS             1875..3182
                     /gene="tig"
                     /locus_tag="VLN25_07870"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HSQ10517.1"
                     /translation="MQQTMDTPGALERRIDLTVPSAMIEKEVQTRLAKLARNVKMPGF
                     RQGKVPMKMVAATYGAKVQAEVMNDKVGEAFNSAVTAGKLRVAGAPRPEPRAVEGSSD
                     LEFSAVFEVYPEIALGDLAGVEVQRAMCTVADAEVDRTLEIMRKQRASYEDVARSAAE
                     GDIATIDFSGSIDGTPFEGGSATDFAFTLGQGRMLPEFEAAVLGMAAGSSKAFSLKFP
                     SDYTAAHLAGKTSEFTVTVKKVQQPVLPPIDADFAKSLGIVDGDLDKMRAEVRANLER
                     EVGGRLKSRTKDSAMSALLSSTSFEVPKSLVEEEQQRLADMTRRDLTQRGVQADKAPL
                     PLDLFAAQADRRVRLGLLVGEVVRTNGLQPRPDQIRKLVEDLAQSYEKPQDVVNWYLS
                     DRKRLAELEGAALEDNVTRWVLEHAKVVDTPVAFDELMGHNQR"
     gene            3208..3855
                     /gene="clpP"
                     /locus_tag="VLN25_07875"
     CDS             3208..3855
                     /gene="clpP"
                     /locus_tag="VLN25_07875"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HSQ10518.1"
                     /translation="MKSHISPHAMAHAIEPQGLGMVPIVIEQSGRGERAYDIFSRLLR
                     DRVVFLVGPVNDQTANLIVAQLLFLESENPDKDISLYINSPGGSVYAGLAVFDTMQFI
                     KPDVQTICVGMAASMGAFLLAAGAKGKRYSLPNSRIMIHQPSGGAQGQASDIEIHAQE
                     ILKTRERLNKILAERTGQPVEKIAKDTDRDNFLSAEDAVSYGLIDKIFIKRGEQP"
     gene            3887..5152
                     /gene="clpX"
                     /locus_tag="VLN25_07880"
     CDS             3887..5152
                     /gene="clpX"
                     /locus_tag="VLN25_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HSQ10519.1"
                     /translation="MSDKKGSSEKLLYCSFCGKSQHEVKKLIAGPSVFICDECIDLCN
                     DIIRDETTTDPGTAGVKSDLPTPREIGGILDQYVIGQDKAKRILSVAVYNHYKRLKHL
                     GSKDDIELAKSNILLIGPTGSGKTLLAQTLARLLNVPFVIADATTLTEAGYVGEDVEN
                     IIQKLLQNCNYEVEKAQKGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     IASVPPQGGRKHPNQDFVQIDTTNILFVCGGAFDGLEKIIRSRSEKSGIGFGATVTSA
                     SERAVGDLLREVEPEDLIKFGLIPELVGRLPVIATLDELGEDALVEILVAPKNALVKQ
                     FQRLFLMEGVELEIRPDALKAIARKAIKRKTGARGLRSIVEGILLDVMYDLPSTKNVV
                     KVVVDENTIGGGKPLLMYADQPKVAGAPR"
     gene            5295..>6311
                     /locus_tag="VLN25_07885"
     CDS             5295..>6311
                     /locus_tag="VLN25_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HSQ10520.1"
                     /translation="MSTQILPPERTTLPLLPLRDVVVFPHMVIPLFVGRPKSIKALEA
                     AMEAGKSIMLVAQKNAAKDEPTAEDIYEIGCMANILQMLKLPDGTVKVLVEGAQRARI
                     ERIEEGVSHFTADVVPIVPDLQPTAEAEALRRAIVSQFDQYVKLNKKIPAKILTSIAG
                     IDEAGRLADTIAAHLPLKLEQKQKILEMHGLNERLEHLLAQLETEIDILQVEKRIRGR
                     VKRQMEKSQREYYLNEQVKAIQKELGEGEEGADLEELEKKIRAARMPKAARDKANSEL
                     KKLKLMSPMSAEATVVRNYLDTLVGLPWKKRSRINNDLTNAEKVLNDDHYGLEKVKDR
                     ILEYL"
BASE COUNT         1263 a         1980 c         2088 g          980 t
ORIGIN      
        1 gatgcgctgg tgcagcacct ggaaatccgc gcgctcgatc tcgtcgagca gggtggcata
       61 gatggcgccc atgatcagcc cagtgcgctg cgccttgcga tcttccgccg gcagcagcgc
      121 cagcgcctcg cggtagaaag cgcgcgcgcg cccggcctgg aagcgcatca gcggcacgaa
      181 tccgtcgacg tagcgagcgt tcagcacgtc ggccgccttg accccgaagc gctgcagttc
      241 gtcgagcggc agataaaggc ggccacggcg tgcatcgtcg ccgacatcgc gaacgatgtt
      301 gatgagctgc agcgcgaggc caagccgctc ggcgtactcg agggtggccg gatggcggta
      361 tccgaagatc gacgccgaca tctcgccgac cacgccggca acgaggtgcg cgtagcgccg
      421 cagcgacgca aagtcgagat agcggttctg gcgcaggtcc atttccatgc cgtcgagcac
      481 ggcgtgcagc cgctgctgcg tgacaccgta tggcccgacg atgaacggct gcagcgcgcg
      541 agtgaccgga tggctgggct gcccgcggac aagggcgtcg atctcgctgc gccaccaggc
      601 gagcttggcc gcggcaaccg tcgggtcact gacctcatcg acgacatcgt cgacctcgcg
      661 gcagtaggcg tagacggccg taatcgcacg ccggcgctct ggcggaagga acaggaacga
      721 gtagtaaaag ctcgaaccgc tgctggctgc cttctgctgg cagtaggcgt ccggattcat
      781 ggagtcagtc gatggcgtca cgtggcggca agcaaggtgg gtgcggcagt cacgtaggag
      841 ccccctgcag actgcgccat cccatgacca gccagtcgcg cgctgccagc aacggacgac
      901 gccggaacac atcgccgtcg cacaggtcga tccgctccag gatgcgcagc ccgccctgaa
      961 ccacgaggcg cagctcgagg ccgatgcgcc ctcgcagcgc gcgtgtcagc gggtcaccgc
     1021 cgtgcagcag ctgacgcgcg cggtcgacct cgaagcgcat cagtgcggtc cagcggtggt
     1081 cgacgtgctg ctcggcgatg tgcgcctcgc cgacgtcgaa tcgctccatg tcctcgcgcg
     1141 ggaggtaaac gcggcccttg ccccagtcga cagcgacgtc ctgccagaaa ttgacgagct
     1201 gcagcccggt gcagatcgaa tccgaccagg ccatcgcttc agcgtcggtt gtctcgtaga
     1261 gtgcgagcaa caggcggccg atcggattgg cggaccggcg gcagtagtcg cgcacgtcgg
     1321 caaacgtggc gtatcgcagc gtgacgacat cctgcgcgaa ggcggagagc agatcgcgga
     1381 agtgagccag cggcaggcga tgcgcccgca ccgccgcggc gaggtccggc cacccgcccg
     1441 actcgccgcg ttcgatcgcg tcgagttcgc tcaccaacgc gtcgagttcg cgcaggcgct
     1501 cggccgggac ggcatcgcct tcgtcggcga tgtcgtcggc cgtccgggca aagcggtaga
     1561 tcgcgcgtat cgccggacgc agcgcaggcg gcgcaagcag cgaagcaacc ggaaaattct
     1621 cgtagtggcc cacgcccatg cgaatgacgc tagcacagcg tttgtgccgc gggccggcgg
     1681 ggcgcttccg ctagaattcg ccgctcgttc cgcgccgagt cgataggcgg ccgcggcgcg
     1741 aaggtggcga aattggtaga cgcaccaggt ttaggtcctg acgccttcac cggcgtgggg
     1801 gttcgagtcc cccccttcgc accaaccgat cctgacccgc cgcgggcacg cggctttcct
     1861 aaaagaatca gaccatgcaa cagactatgg ataccccggg cgcgcttgag cgtcgcatcg
     1921 acctgacggt tccgtcggcg atgatcgaga aggaagtgca gacgcgcctg gccaagctgg
     1981 cgcgcaacgt gaagatgccg ggctttcggc agggcaaggt gccgatgaag atggtcgccg
     2041 cgacctacgg cgccaaggtg caagccgaag tcatgaatga caaggtcggc gaagcgttca
     2101 actcggcggt cacggcggga aagctgcgcg tggccggtgc gcctcgcccc gagccgcgtg
     2161 cggtggaagg gtcgagcgac ctggagttct cggcggtctt cgaggtgtat ccggagatcg
     2221 ccctcggcga cctggcgggc gtggaagtcc agcgggctat gtgcacggtg gcggatgcgg
     2281 aagtcgaccg caccctcgaa atcatgcgca agcagcgcgc cagctacgag gacgtggcgc
     2341 gcagtgccgc cgagggggat atcgcaacca tcgacttcag cggttcgatc gacggtacgc
     2401 cgtttgaagg tggcagtgcg accgacttcg ccttcacgct gggccaggga cgcatgctgc
     2461 ccgagttcga ggccgcggtg ctgggcatgg cggccggttc gagcaaggcc ttttcgctga
     2521 aattcccctc ggattacacg gcggcacacc tggccggcaa gacgtcggag ttcactgtca
     2581 cggtgaagaa ggtgcagcag ccggtgctgc cgccgattga cgccgacttc gcgaagtcgc
     2641 tcggcatcgt cgacggcgat ctcgacaaga tgcgggccga ggtccgggcc aacctcgaac
     2701 gcgaggtcgg cgggcggctg aagtcgcgta cgaaggacag tgccatgtcg gcgctgctga
     2761 gctcgacgag ctttgaggtg ccgaagtccc tggtcgagga ggagcagcag cggctcgccg
     2821 acatgacgcg gcgtgatctg acgcagcgcg gcgtgcaggc tgacaaggcg ccgttgccgc
     2881 tggacctgtt tgccgcgcag gctgaccgcc gcgtgcggtt gggcctgctt gtcggagaag
     2941 tcgtgcgcac gaacggcctg cagccgcgac ccgaccagat ccgcaagctc gtcgaggacc
     3001 tcgcccagag ctacgagaag cctcaggatg tcgtcaactg gtatctgtcg gaccgcaagc
     3061 gactggccga gctggaaggc gcggcgctcg aggacaatgt gacacgctgg gtgctggaac
     3121 acgcgaaggt cgtcgacacg ccggtcgcgt tcgacgaact gatgggacat aaccagcgct
     3181 aatgcgttgc agccaggaga caaccggatg aaaagccata tcagccccca cgccatggcc
     3241 catgcgatcg aaccgcaggg tctcggcatg gtgccgatcg tcatcgagca gagcggtcgc
     3301 ggcgagcgcg cctacgacat cttctcgcgc ctgttgcgcg accgggtcgt tttcctcgtc
     3361 gggccggtca atgaccagac cgcgaatctc atcgtggcac agctgctgtt cctggagtcc
     3421 gagaatccgg acaaggacat ttcgctgtac atcaactcgc ccggcggctc ggtctatgcg
     3481 gggctcgcgg tcttcgacac gatgcagttc atcaagcccg acgtacagac gatctgtgtc
     3541 ggcatggccg cgagcatggg ggcgttcctg ctggccgcgg gcgccaaggg caagcgctac
     3601 tcgctgccga attcgcgcat catgattcac cagccgtccg gaggcgcgca gggccaggcc
     3661 agcgacatcg agatccatgc gcaggagatc ctgaagacgc gcgagcggct caacaagatc
     3721 ctcgcggaac ggaccggtca accggtcgaa aagatcgcca aagacaccga tcgcgacaac
     3781 tttctgtcgg ccgaggatgc agtaagctat ggattaatcg acaagatctt catcaagcgc
     3841 ggcgagcagc cgtagcccgc gccgtccgga gaaacgtccc agacccatgt cggacaagaa
     3901 gggttccagc gaaaagctgc tctactgttc cttctgcggg aagagtcagc acgaagtcaa
     3961 aaagctcatc gccggcccgt cggtgttcat ctgcgacgag tgcatcgatc tctgcaatga
     4021 catcatccgt gacgagacga caacggaccc gggcaccgcc ggcgtcaagt cggatctgcc
     4081 cacgccgcgc gagatcggcg gcatcctcga ccagtacgtg atcggacagg acaaggccaa
     4141 gcgcatcctg tcggtagccg tctacaacca ctacaagcgg ctcaagcatc tcggctcgaa
     4201 ggacgacatc gagctcgcca agagcaacat cctgctgatc ggaccgaccg ggtcgggcaa
     4261 gacgttgctg gcccagacgc tggcgcggct gctcaatgtc ccgttcgtga ttgcggatgc
     4321 gacgacactg accgaagccg gatacgtcgg cgaggacgtc gagaacatca tccagaagct
     4381 gttgcagaac tgcaattacg aagtcgagaa ggcgcagaag ggcattgtct acatcgacga
     4441 gatcgacaag atctcgcgca agagcgacaa cccctccatc acgcgcgacg tctcgggcga
     4501 aggcgtgcag caggcgttgc tcaagctgat cgagggcacg atcgcctcgg taccgccgca
     4561 gggcggccgc aaacatccga accaggactt cgtccagatc gacacgacca atattctgtt
     4621 cgtctgcggc ggcgccttcg acgggttgga gaagattatt cgcagccgct cggaaaagag
     4681 cggcatcggt ttcggcgcga ccgtcaccag tgccagcgag cgcgccgtcg gcgacttgct
     4741 ccgcgaggtc gaaccggaag acctcatcaa gttcgggctc attccagaac tggtgggtcg
     4801 cttgccggtc attgcaaccc tggatgaact gggcgaggat gcgctcgtcg agatcctggt
     4861 cgcccccaag aatgcgctgg tcaagcagtt ccagcggctg ttcctcatgg agggtgtcga
     4921 actggagata cggcccgacg cgctcaaggc gatcgcgcgc aaggcgatca agcgcaagac
     4981 cggcgcgcgt ggactgcgtt cgatcgtcga aggcatcctg ctcgacgtca tgtacgacct
     5041 gcccagcacc aagaacgtcg tcaaggtggt cgtcgacgag aacacgatcg gcggcggcaa
     5101 gccactgctc atgtacgccg accagcccaa ggttgccggg gcgccgcgct agtccggcgc
     5161 cgtcaggcgt cccgggccgc gatgatgctc atcgcgtgct agattgaaac gcactgaaga
     5221 ttcagcgggc ttgctttgcc cctgcgcgga cccatatgcc tcgctgaacc ttccttattg
     5281 agggacaaat ccccatgtcg acccagatcc tgccgccgga acgcacgacg ctgccgctgt
     5341 tgccgctgcg cgatgtcgtg gtgtttccgc acatggtcat tccgctgttt gtcgggcggc
     5401 cgaagtccat caaggcgctc gaggcggcga tggaggcagg caagagcatc atgctggtgg
     5461 cgcagaagaa tgccgccaag gacgagccga ccgccgagga catctacgag atcggctgca
     5521 tggccaacat cctgcagatg ctgaagctgc cggatggcac cgtgaaggtg ctggtcgagg
     5581 gtgcgcagcg cgcccgcatc gaacggatcg aggagggtgt cagccacttc accgccgacg
     5641 tggtcccgat cgtgcccgac ctgcaaccga ccgcagaggc cgaggccctg cgccgtgcga
     5701 tcgtcagcca gttcgaccag tacgtgaaac tgaacaagaa gatcccggca aagatcctga
     5761 cctcgattgc cggcatcgac gaggccggcc gcctggccga cacgatcgcg gctcacctgc
     5821 cgctcaagct cgagcaaaag cagaagatcc tggagatgca cgggctgaat gaacgcctcg
     5881 agcatctgct cgcgcaactc gagaccgaga tcgatatcct gcaggtcgaa aagcgcatcc
     5941 gcggccgcgt caagcggcag atggagaaga gccagcgcga gtactacctg aacgagcagg
     6001 tcaaggcgat acagaaggaa ctgggcgagg gtgaggaagg tgccgatctc gaggaactcg
     6061 agaagaagat ccgcgctgcc cgcatgccga aggccgcgcg cgacaaggcg aactcggagc
     6121 tgaagaagct caagctgatg tcgccgatgt cagccgaagc caccgtcgtg cgcaactacc
     6181 tggacacgct cgtgggtctg ccgtggaaga agcgcagccg tatcaacaac gacctgacga
     6241 acgccgaaaa ggtcctgaac gatgatcatt acggactcga gaaggtcaaa gaccgcatcc
     6301 tcgagtacct c
//
LOCUS       DATIRT010000347         2174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_644723, whole genome shotgun sequence.
ACCESSION   DATIRT010000347 DATIRT010000000
VERSION     DATIRT010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644723"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VLN25_07890"
     CDS             <1..453
                     /locus_tag="VLN25_07890"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HSQ10521.1"
                     /translation="HEVGLAMPNYLEAVTKGLIKRAATLRDLADTLGVDRNGLEQSVA
                     LVNALAFEDDMDEFGRSFHASQMILGPYYGVQVTGALLGTEGGLAIDASGQVLRPDGT
                     TLPNLLAAGGAARGLSGDGGGGYLEGNGLLAAVVGGYLAGRKAAELAY"
     gene            complement(487..882)
                     /locus_tag="VLN25_07895"
     CDS             complement(487..882)
                     /locus_tag="VLN25_07895"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009936363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HSQ10522.1"
                     /translation="MTLPQQLPDDQAPVLRMMPMPADSNIHGDVFGGWIMAHVDIAGA
                     VPAARRASGRVATVAVTSFLFKQPVFVGDLVSFYANIVKTGRTSVTVDVEVYVERNRL
                     QKEVVKVTEATLVYVATDERRQPRALPAL"
     gene            complement(905..1726)
                     /locus_tag="VLN25_07900"
     CDS             complement(905..1726)
                     /locus_tag="VLN25_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSQ10523.1"
                     /translation="MRFEGLVVLITGASDGIGAEIARQLAKENARLVLAARTRETLER
                     VGAECRELGAEALVVPTDVTVEDDCRRLVERAVQSFGRIDILVANAGVSMHARFSQIE
                     DFSTFERLFRINAMGTIWCVRHAYPYLKTSRGLIVGVSSMAGRTGVPERTTYCTSKFA
                     QTGFLDALRIEAADDGIDVMVAYPGVVATEIRRRGWNGRGEPARVSGLSEAHAMSTDV
                     CARQIIDGMRVRRREVVMSARERVGLWLKLIAPAIVDRMARAALAKPTDRSTSPH"
BASE COUNT          360 a          736 c          733 g          345 t
ORIGIN      
        1 cacgaggtcg gcctggccat gcccaattac ctggaagccg tcaccaaggg gctgatcaag
       61 cgcgccgcga cgctgcgcga tcttgcggac acgctcggcg tggatcgcaa cggcctcgag
      121 cagagcgtcg ccctggtgaa cgcactggcg ttcgaagacg acatggacga gttcgggcga
      181 tcgtttcacg cctcgcagat gattctcggt ccgtactacg gcgtgcaggt caccggcgcg
      241 ctgctgggca ccgaaggcgg tctggcgatc gacgccagcg gccaggtgct gcggccggac
      301 ggcacgacgc tgcccaacct gctggccgcg ggtggcgccg cgcgcggact gtcgggtgac
      361 ggcggcggcg gctacctcga aggaaacggc ctgctggcgg ctgtcgttgg cggttatctc
      421 gcggggcgca aggccgccga actcgcgtat tgacggcgac cgagcgcgag cgggtcgttc
      481 gcgtcgctac agcgccggca aggcgcgcgg ctggcgacgt tcgtcggtgg ccacgtagac
      541 cagcgtcgcc tctgtcacct tgacgacctc cttctgcagg cggttacgct cgacatagac
      601 ctcgacatcg accgtgaccg acgtgcggcc ggtcttgacg atgttggcgt agaagctcac
      661 caggtcgccg acgaataccg gctgcttgaa caggaacgac gtgaccgcca ccgtcgccac
      721 gcgaccggag gcacgccgtg ccgccggaac cgcgccggcg atgtcgacat gcgccatgat
      781 ccagccgccg aacacgtcgc cgtggatgtt ggaatcggcg ggcatcggca tcatgcgaag
      841 caccggagcc tgatcgtcgg gcaattgctg aggtagcgtc attggcgtgt cgtccgggcg
      901 agcgtcagtg aggggaggtc gaacggtccg tcggcttcgc caaggcggcc cgcgccatgc
      961 gatcgacgat ggccggagcg atcagcttca gccacaggcc gacccgctca cgtgcgctca
     1021 tcacgacctc gcgacgccgg acgcgcatgc cgtcgatgat ctggcgggcg cagacatcgg
     1081 tcgacatcgc atgggcctcc gagaggcccg ataccctggc gggctcgccg cggccgttcc
     1141 agccgcgacg acggatctcg gtggcgacca cgccgggata ggccaccatc acgtcgatgc
     1201 cgtcgtcggc ggcctcgatc cgaagggcgt cgagaaagcc cgtctgtgca aacttgctgg
     1261 tgcagtaggt cgtgcgctcg ggaacgccgg tccgccccgc catcgacgac actcccacga
     1321 tcagcccgcg tgacgtcttc agataaggat aggcgtggcg cacgcaccag atcgtcccca
     1381 tcgcattgat ccggaacagt cgctcgaagg tcgagaaatc ctcgatctgc gagaagcgcg
     1441 catgcatcga gacgccagcg ttggccacca ggatgtcgat gcgtccgaac gactggacgg
     1501 cgcgctcgac gaggcggcgg cagtcgtctt cgaccgtcac gtccgtcggc accacgaggg
     1561 cctccgcgcc gagctcccgg cactcggcgc cgacccgttc cagcgtctcg cgcgtgcgtg
     1621 ccgccagcac cagccgcgcg ttctccttcg ccagctgacg cgcgatctcc gctccgatgc
     1681 cgtcactggc accggtgatc aagacaacaa gaccttcgaa cctcatgact ctgcgtgtgg
     1741 ataatcgcga aatgacagcg aatcgcacca agtgtacgca tcgacctggc gtacgcggtt
     1801 ccattcagtc gtggccaatg ggtcaagatc agcagacggc acgggccccc ctcgcgtggc
     1861 gagggggaag ggggagtgcc gtgcagttgt atttcggcgg agcgcgcggc cgctccgaag
     1921 cgcagccgac cgaccttttc ttgccggtcc gagcgcacgt gcgatgcgcg cagcgcatcg
     1981 cgtacgcgag aatcgcctag ctatgcgctg gtccccccct cgcgaagccc tgccgtcgac
     2041 gcgtgcggag cgcgacgact ggcagaccct gcgcatgctg ctgccttacc tgtgggccta
     2101 cccggggagg gtgctgtttg cactggcgtg tctggtggcc gccaagctcg ccaccgtcag
     2161 cgtcccgatc ctgc
//
LOCUS       DATIRT010000348         2769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_152223, whole genome shotgun sequence.
ACCESSION   DATIRT010000348 DATIRT010000000
VERSION     DATIRT010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152223"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /gene="hisB"
                     /locus_tag="VLN25_07905"
     CDS             complement(<1..514)
                     /gene="hisB"
                     /locus_tag="VLN25_07905"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HSQ10524.1"
                     /translation="MTARVSPRRVTVERNTKETQIRVELDLDGNGHCQVSSGVGFFDH
                     MLDQIARHGMLDLVIDARGDLHIDGHHTVEDIGITMGQAFTQAIGDKTGVRRYGHAYV
                     PLDEALSRVVIDLSGRPGLVWNVPFKRAMIGEFDVDLTHEFFQGFVNHALVTLHVDNL
                     RGDNAHHQCET"
     gene            complement(511..1605)
                     /gene="hisC"
                     /locus_tag="VLN25_07910"
     CDS             complement(511..1605)
                     /gene="hisC"
                     /locus_tag="VLN25_07910"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HSQ10525.1"
                     /translation="MNARIGQRPNLSVLRDDVRALEAYAVPSAVGFIKLDAMENPYTL
                     PEALQRQLGERLAGQPLNRYPLSDPRDFKERLGRAMGLPPGARLMLGNGSDELIHLMI
                     QACARPGATIMSPWPSFSMYELSTVYDGCRFVGVPLRADFTLDSRALLAAVAVHRPAV
                     IFIAYPNNPTGNLFDRNDIEAILRAAPGLVVIDEAYLPFAQDTWMPQLHSWPNLVVLR
                     TLSKLGLAGIRLGYLAGDPEWIAEFDKLRPPYNVNVLTLATADFLLEHLATLEAQAAM
                     LRAERSRVAAALAALRGITVFPSSANFLLFRVDDAERTFNGLKERGILIKNVSKMHPL
                     LAGCLRTTIGTAVENDAFLQGLQAAMESSR"
     gene            complement(1605..>2769)
                     /gene="hisD"
                     /locus_tag="VLN25_07915"
     CDS             complement(1605..>2769)
                     /gene="hisD"
                     /locus_tag="VLN25_07915"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HSQ10526.1"
                     /translation="THRFDRIAAARVAYLEIPQEAMRVAFEDLPSEQSEALQAAADRI
                     RAFHREQHARLMQGDWITTEADGTITGQRITPLDSVGLYVPGGKAAYPSSVLMNSIPA
                     HVARVERLIMVVPTPDGERNALVLAAAHLGGVSRAFAVGGAQAVEALAYGTATVPAVD
                     KIVGPGNAYVAAAKRRVFGTVGIDMIAGPTEILVIADGDANPDWIAMDLFSQAEHDEL
                     AQALLLSPDAGLIAAVEASIRRLLPGQTRRDIIAASLANRGALVHTRDLAEAVALCNR
                     VAPEHMEIVTSDPEALLPEIRHAGAIFVGHYSSESLGDYCDGPNHVLPTVRTARFSSP
                     LGVYDFVKRSNLIHVSAAGAQRLGPIASVLARGEHLTAHALSAELRLDVAPGK"
BASE COUNT          466 a          918 c          943 g          442 t
ORIGIN      
        1 cggtttcgca ctggtggtgc gcgttgtcgc cgcgcaggtt gtcaacgtgc agcgtgacca
       61 gcgcgtgatt cacgaagccc tggaagaatt cgtgcgtcag atcgacgtcg aattcgccga
      121 tcatcgcccg cttgaagggc acgttccaca cgaggcccgg ccggccggac aggtcgatca
      181 cgacgcgcga cagcgcctcg tccagcggca cgtaggcgtg gccatagcgc cgcacgccgg
      241 tcttgtcccc gatcgcctgc gtgaacgcct ggcccatcgt gatgccgatg tcctcgaccg
      301 tgtgatgccc gtcgatgtgc aggtcgccgc gggcgtcgat gacgaggtcg agcatgccgt
      361 gacgcgcgat ctgatccagc atgtgatcga agaacccgac gccggagctg acctggcagt
      421 ggccattgcc gtcgaggtcg agctcgacgc gaatctgggt ttccttcgtg ttgcgctcca
      481 ccgtaacgcg gcggggtgag actcgagcgg tcatcgtgag ctttccatgg cggcctgcag
      541 cccctgcagg aacgcgtcgt tttccacggc ggtgccgatt gtcgtgcgca gacacccggc
      601 gagcagcgga tgcatcttcg agacattctt gatgagtatg ccgcgctcct tcagtccgtt
      661 gaacgtccgt tcggcgtcat cgacacgaaa cagcaggaaa ttcgccgacg acggaaacac
      721 cgtgatgccc cgcagggcgg cgagtgcagc ggccacgcgc gagcgctcgg cacgcagcat
      781 ggcagcctgc gcttcgagcg tggccaggtg ttcgagcagg aaatcggccg tggccagcgt
      841 caggacgttg acgttatagg gcggacgcag cttgtcgaac tccgcgatcc attccggatc
      901 gccggccagg taaccgagcc ggatgccggc cagccccagc ttggacagcg tgcgcagcac
      961 caccaggttg ggccagctgt gcagctgcgg catccaggtg tcctgcgcaa acggcaggta
     1021 agcctcgtcg atgacgacta gcccgggggc cgcgcgcagg atcgcctcga tgtcgttgcg
     1081 gtcgaagagg ttccccgtcg gattgttcgg gtaggcaatg aagattaccg ccggccggtg
     1141 caccgccacg gctgccagca gcgcgcgact gtcgagcgtg aaatcggcac gcagcggaac
     1201 gccgacgaag cggcatccgt cgtaaaccgt cgacaactcg tacatcgaga agctcggcca
     1261 tggcgacatg atggtcgctc ccgggcgcgc gcaggcctgg atcatcaggt gaatgagttc
     1321 gtccgagccg ttgcccagca tcaggcgagc accgggcggg agacccatcg ccctgccgag
     1381 ccgctccttg aagtcgcgcg gatcagacag cgggtagcgg ttcagcggtt ggcctgccag
     1441 ccgctccccg agttgccgct gcagagcttc gggcagcgtg tagggattct ccatcgcgtc
     1501 gagcttgatg aagccgaccg cgctaggcac cgcataggcc tcgagcgcgc gcacgtcatc
     1561 gcgcaggacc gagagattcg ggcgttgccc gatgcgcgcg ttcatcactt ccccggcgcg
     1621 acgtccaggc gaagctcggc ggacagcgcg tgcgccgtaa ggtgctcgcc acgtgcgagc
     1681 accgaggcga tcggaccgag gcgttgagcg ccggcggccg acacgtggat caggttcgac
     1741 cgcttgacga agtcgtacac gccgagcggc gaggagaagc gcgcagtgcg gacggtgggc
     1801 agcacgtggt ttggcccgtc gcagtagtca ccgagcgact ccgacgaata gtgccccaca
     1861 aagatcgccc ccgcatgccg gatctccggc aacaacgcct cggggtccga cgtgacgatc
     1921 tccatgtgct cgggcgccac gcggttgcac agtgcaacgg cttcagcaag atcgcgtgtg
     1981 tggaccagcg cgccgcgatt ggcaagcgac gccgcgatga tgtcgcgccg cgtctggccc
     2041 ggcagcagcc gccggatcga ggcctcgacc gcggcgatca gtccggcgtc cgggctcagc
     2101 agcagggcct gcgccagttc gtcgtgctcc gcctgcgaga acaggtccat cgcgatccag
     2161 tcgggattgg catcgccgtc ggcaatcacc aggatctcgg tcgggccggc gatcatgtcg
     2221 atgccgaccg tgccgaagac gcgccgcttc gctgccgcga cataggcatt gccgggcccc
     2281 acgatcttgt cgaccgccgg cacggtcgcc gtgccgtaag cgagcgcctc cacggcctgt
     2341 gcgccgccca ccgcaaaggc gcgcgagacg ccgcccaggt gcgcggcggc gagcaccagc
     2401 gcgttgcgct cgccatccgg cgtcggtacg accatgatca gccgctcgac gcgggcgaca
     2461 tgggcaggaa tcgaattcat cagcaccgac gacgggtagg ccgccttgcc gccgggcacg
     2521 tacaggccaa cgctgtcgag cggcgtgatg cgctggccgg tgatcgtccc gtcggcttcc
     2581 gtcgtgatcc agtcaccttg catcaacctg gcgtgctgct cgcgatggaa ggcgcggatg
     2641 cggtctgccg ccgcctgcaa cgcctcgctc tgttcggaag gcagatcctc gaaggccacg
     2701 cgcatcgcct cctgcgggat ctccaggtac gccacccggg ccgccgcgat gcggtcgaaa
     2761 cggtgggtg
//
LOCUS       DATIRT010000349         7164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1038303, whole genome shotgun sequence.
ACCESSION   DATIRT010000349 DATIRT010000000
VERSION     DATIRT010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7164
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038303"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /gene="murG"
                     /locus_tag="VLN25_07920"
     CDS             <1..970
                     /gene="murG"
                     /locus_tag="VLN25_07920"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HSQ10527.1"
                     /translation="GTQAGIERGLVERHGIAFDAIDFSGLRGKGLKTLLLGGFMLLRA
                     LWQSRARLRARAPKVVFSTGGYVAVPAGMAASSLGVPLVLMNADAETLLSTRLLTPLA
                     AGVLCGFDGAAAVKAGDKGLVTGNPVRSEIAALPVPEQRYVGRTGPLNLLVIGGSLGA
                     KVLNETVPAALARLPIERRARVVHQCGAGHLEAVQAAYRRAGVDADILPFIDDLAQRY
                     ALADVVLCRAGAITITELAVAGVPAILVPLVVSTTQHQRTNAEYMVGKGAALHLAQSE
                     FTAERLADVLDGLSRERLLGMANAARAVGRPDATNTVARVIERMAA"
     gene            967..1089
                     /locus_tag="VLN25_07925"
     CDS             967..1089
                     /locus_tag="VLN25_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10528.1"
                     /translation="MKHAVKHIHFVGFDGCVGGGAPTSLSTSMPLRPDALEACR"
     gene            1086..2477
                     /gene="murC"
                     /locus_tag="VLN25_07930"
     CDS             1086..2477
                     /gene="murC"
                     /locus_tag="VLN25_07930"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007747070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HSQ10529.1"
                     /translation="MKHAVKHIHFVGIGGSGMSGIAEVLLTLGYRVSGSDVASTAVTR
                     RLAQLGAVIAQGHAAEHIAGADAVVISTAVSPSNPEVIAARGRRIPVVPRAVMLAELM
                     RLKQGIAIAGTHGKTTTTSLVASVLAAGGMDPTFVIGGRLNSAGANARLGSGEYIVVE
                     ADESDASFLNLMPVIAVVTNIDNDHMETYGHDFSRLKHAFIEFLGRLPFYGSAVLCID
                     DPHVRDILPFVSKPVLSYGFAPEAQVRAIDAVADGTRMRFTVQREDQPPLAIVLNLPG
                     RHNVANALAAIAVGSELGVSDSAIAEALAGFTGVGRRFALHGEQPATRGGSYTLIDDY
                     GHHPVEMAATIAAVRDAFPGRRLVLAFQPHRYTRTRYCFEDFVKVLSTTDVLLLAEVY
                     AAGEAPIVAADSRSLARALRVAGKVEPIFVETIDELPAALHDVVRDGDVVITMGAGSI
                     GQVPARLAGGGAE"
     gene            2474..3430
                     /locus_tag="VLN25_07935"
     CDS             2474..3430
                     /locus_tag="VLN25_07935"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HSQ10530.1"
                     /translation="MSAQPTSFGKVGVLFGGRSSEREVSIMSGTGVLNALKSRGVDAQ
                     AFDPGRQSLAELEASRFDRVFIALHGRGGEDGTIQGVLETLEVPYTGSGVQASAIAID
                     KETTKQIWLQAGIPTPAHRIGHAETDWMQVVAEIGDQLIVKPSREGSTIGITKVTTHD
                     RNELALAFESATQYDDTVLIEQLIAGRELTCAILGEGEEAEALPLIEIRAPGGNYDYH
                     SKYFSDDTKYLCPAPIDADIAERICTLCLRSYRAVGARGWGRVDVMLRDDGAPFLIEL
                     NTSPGMTGHSLVPMAAREAGMSYEDLVLRILGTASLDSRRRG"
     gene            3427..4182
                     /locus_tag="VLN25_07940"
     CDS             3427..4182
                     /locus_tag="VLN25_07940"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HSQ10531.1"
                     /translation="MIGARIMNLAALVAALIAIGLLLSTGTRWLTQRPAFDFKRIEVR
                     GELQHVTAASIRAAIAGRLRGNYFTVRLDETRRLLETVPWISQAAVRRIWPNRLQVTL
                     REHRALGAWGDGRLLSDRGELFVANIAEAEIFGPLPSFAGPAIAAAEVAARYYDFAAL
                     VAELSLSIDGIDVSDRRSWSIQASGPGVAPTTLELGREDSAGAVHRRLAHVVAAYPMV
                     SARVGGPPARIDARYANGIAASSPSSSTVPKAR"
     gene            4186..5418
                     /gene="ftsA"
                     /locus_tag="VLN25_07945"
     CDS             4186..5418
                     /gene="ftsA"
                     /locus_tag="VLN25_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HSQ10532.1"
                     /translation="MRTETKDLIVGLDIGTSKVVVAVAEALPDGRFQVIGLGQAASQG
                     LRKGVVVNIESTVQSIRRALEEAELMAACQIKDAFTGIAGSHIKSFNSSGMVAIKDRE
                     VTASDVARVIETAKAINIPTDQQVLHVLTQEFIVDGQEDIREPIGMSGVRLEVRVHIV
                     TGAVSAAQNIIKCVRRCGLEVQDLILQPLASSLSVLTADEKELGVALVDVGGGTSDIA
                     IFTGGAIRHTAIIPIAGDQITNDIAMALRTPIPDAEEIKVRWGVAKEVLADPNEKIEI
                     PGLGDRGPRLLSRQALAAVIEPRVEELFTLTQQVIRESGYEEMLSSGVVLTGGTSLLP
                     GMTELAEDVFLKPARIGWPQYDGSLADVVKNPRFATAMGLLVEAHSQRQRGRKIAHQT
                     GSFSQTFKRMKEWIVGNF"
     gene            5546..6730
                     /gene="ftsZ"
                     /locus_tag="VLN25_07950"
     CDS             5546..6730
                     /gene="ftsZ"
                     /locus_tag="VLN25_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HSQ10533.1"
                     /translation="MTFEILETETRGTIIKVVGVGGAGGNAVEHMIRRSVQGVEFICA
                     NTDHQALARSSSKNVLQLGKTGLGAGSRPEAGRQAAEEARDRIADSLRGAHMVFITAG
                     MGGGTGTGAAPVVAEVAKELGLLTVAVVSKPFEFEGPKRLKVAETGLAELEASVHSLI
                     VILNNKLEEVMGEDAEMADCFRAADDVLHNACAGIAEIINVPGLVNVDFEDVKTVMSE
                     RGKAMMGTATASGLDRARIAAEQAVASPLLEGVDLSGARGVLVNITASSSLKMKETRE
                     VMNTIRAFAADDATVIFGTVCDESIGDGLRVTVVATGLGRAGSKKQSNPFTVVRTGTD
                     NAPLMVAPTRETATAGSDYSHLDTPAVWRSRESAQQKVSAMEESGSDRYDIPAFLRKQ
                     AD"
BASE COUNT         1239 a         2264 c         2472 g         1189 t
ORIGIN      
        1 cggcacgcaa gccggaatcg agcgaggcct ggtcgagcga catggcatcg cgttcgacgc
       61 aattgacttc agcggcctgc gcggcaaggg cctcaagacc ctgctgctcg gcggattcat
      121 gctgctgcgc gccctgtggc agagccgtgc acgtctgcgc gcgcgtgcac ccaaggtcgt
      181 gttttcaacc ggaggctacg ttgccgtgcc ggccgggatg gcggcaagct ccctcggcgt
      241 gccgctggtc ctgatgaacg ccgacgccga gacgctgctg tcaacgcggc tgctgacgcc
      301 gcttgcggcc ggcgtgctgt gtggattcga tggtgcagcc gccgtgaagg cgggtgacaa
      361 ggggctggtt accggcaatc cggtacggtc cgaaatcgcc gcgctaccgg tgccggagca
      421 acggtacgtg ggacgcacgg ggccattgaa cctgctggtg atcggcggca gcctcggcgc
      481 gaaggtgctc aatgaaacgg tgccggccgc gcttgcacgg ctaccgatcg agcgtcgtgc
      541 gcgcgtcgtg caccagtgcg gagccgggca ccttgaggct gttcaggccg cctaccgccg
      601 ggccggcgtc gacgccgaca tccttccgtt catcgacgac cttgcgcagc gctacgcgtt
      661 ggccgacgtg gtgctgtgtc gtgccggtgc gatcacgatc accgagcttg cggtggcggg
      721 cgtcccggcc atcctggtgc ctctcgttgt atcgacgacc cagcaccagc gcaccaacgc
      781 cgagtacatg gtcggcaagg gcgcagcgct gcatcttgcg caaagcgagt tcacagcgga
      841 acgcctagcg gacgtgctcg acggactttc gcgtgaacgg ctgctcggga tggccaacgc
      901 cgcccgtgct gtcggtcggc cggatgccac gaacaccgtg gcccgggtca tcgaacggat
      961 ggctgcatga agcacgccgt caagcacatt cacttcgtcg gcttcgacgg ctgtgtcggc
     1021 ggcggggccc cgacgtcctt gagtacttcc atgccgctgc gaccggacgc gctcgaggcc
     1081 tgtcgatgaa gcacgccgtc aaacatatcc acttcgtcgg catcggcggc agcggcatga
     1141 gtggcattgc cgaagtgctg ctgacgctcg gttaccgcgt cagcggctcg gatgttgcga
     1201 gcactgcagt gacgcggcgg cttgcccagc tcggtgcggt gatcgcccaa gggcacgcgg
     1261 ccgagcatat cgccggcgcg gacgccgtcg tgatctcgac cgcagtgtcg ccgagtaatc
     1321 cggaagtgat cgcggcgcgc ggccggcgta tcccggtcgt accgcgtgcc gtgatgctcg
     1381 cggaactgat gcgactgaag cagggcatcg cgattgccgg gacacacggc aagacgacga
     1441 ccaccagcct ggttgcaagc gtgctggcgg cgggcgggat ggatccgacc ttcgtgatcg
     1501 gtgggcgcct gaattcggcc ggcgcgaacg cacggctcgg cagcggcgag tacatcgtcg
     1561 tcgaggccga cgagtcggac gcatcctttc tcaacctgat gccggttatc gctgtcgtaa
     1621 cgaacatcga caacgatcac atggagacct acggacacga tttttcgcgg ctcaaacacg
     1681 cgttcatcga gttcctgggc cgcttgccct tctacggctc ggccgtgctg tgcatagacg
     1741 atccccatgt gcgcgacatc ctgccgttcg tgtccaagcc ggtgctgagc tacggctttg
     1801 cacccgaggc ccaggtgcgt gcgatcgacg cagtcgcgga cggaacgcgc atgcgcttca
     1861 ccgtgcagcg cgaagaccag ccgccgctgg cgatcgtcct caatctcccg ggacgccaca
     1921 acgtggccaa tgcactggcc gcgatcgcgg tcggcagtga gctgggcgtg agcgattcgg
     1981 cgatcgccga ggcgctggcc ggatttacgg gggtggggcg acgctttgcc ctgcacggcg
     2041 agcagccggc cacgcgcggc ggctcgtaca ccctgatcga cgattatgga caccacccgg
     2101 tggagatggc cgcaacgatc gcggcggtgc gcgacgcgtt tccggggcgg cgtctcgtcc
     2161 tcgcgttcca gccgcaccgc tacacccgca cgcgctactg cttcgaagac ttcgtcaagg
     2221 tgctgtcgac caccgatgtg ctgctgctgg ccgaggtcta tgccgcaggt gaagcgccga
     2281 tcgtcgctgc cgatagccgc tcgctggcgc gtgccctgcg cgtggcgggc aaggtcgagc
     2341 ccattttcgt cgagacgatc gacgagcttc cggcagcact gcacgatgtc gtgcgcgacg
     2401 gcgatgtcgt gatcacgatg ggcgctggct cgatcgggca ggtgccggcg cggctggcag
     2461 gagggggtgc ggaatgagcg cgcaaccgac ctccttcggc aaggtcggcg tgctgttcgg
     2521 cggccgttcg tccgaacgcg aagtgtcgat catgtcgggt accggcgtac tcaacgcgct
     2581 gaagagccgg ggcgtcgatg ctcaagcctt cgatcccggc cgtcaatcac tggcagagct
     2641 cgaagcatcc cggttcgatc gggtgttcat cgcgctgcat ggacgtggcg gcgaggacgg
     2701 cacgatccag ggcgttctcg agacgctcga ggttccgtac acgggcagcg gcgtacaggc
     2761 gtcggcgatt gcgatcgaca aggaaaccac caagcagatc tggctacagg cgggcatccc
     2821 gacgccggcg caccgcatcg gacacgccga gacggactgg atgcaagtcg tcgctgaaat
     2881 aggcgaccag ctgatcgtca agccgtcgcg ggaagggtcg accatcggca tcaccaaggt
     2941 cacgacccat gatcgcaacg aactcgcgct cgccttcgaa tcggcaaccc agtacgacga
     3001 cacggtcctg atcgagcagc tgatcgcggg gcgggaactc acctgcgcaa tcctgggtga
     3061 gggtgaggag gccgaggcat tgcccctgat cgagatccgt gcacccggcg gcaattacga
     3121 ctaccacagc aagtacttca gcgacgacac gaagtacctg tgtccggctc cgatcgacgc
     3181 ggacatcgcc gaacgcatct gtactctgtg cctgcgctcg tatcgcgcgg tgggcgcgcg
     3241 gggctggggc cgcgttgacg tgatgctgcg cgacgacggc gcgccgttcc tgatcgagct
     3301 caatacctcg ccgggcatga ccggccactc gctcgtaccg atggcggcgc gggaggcggg
     3361 catgagctac gaggatctgg tgctgcgcat cctcggcacc gcctcgctcg attcgcggag
     3421 gcgcggatga tcggggcgcg gatcatgaac ctcgctgcgc tggtggccgc gctgattgcc
     3481 atcggtctgc ttctgtcgac cggcacacgc tggcttacgc agcgccccgc gttcgatttc
     3541 aagcgcattg aagtgcgtgg cgaactgcag catgtgacgg cggcttcgat acgcgccgcc
     3601 atcgccggcc ggctcagggg caactacttc acggtacgcc tggacgaaac gcgtcgcctg
     3661 ctcgaaaccg tgccgtggat atcccaggcc gcggtgcgga ggatctggcc caaccggctg
     3721 caggtgacgc tgcgcgaaca tcgggctctg ggtgcgtggg gcgacgggcg cctgttgtcg
     3781 gatcgcggcg aactgttcgt cgcgaatatc gccgaggcag aaatcttcgg accgctgcca
     3841 tcgttcgccg ggccggccat cgcggctgcc gaggtggcgg cgcgctacta cgactttgcc
     3901 gcgctggtcg ccgaactttc gctgtcgatc gacggcatcg acgtgtcgga tcgccgctcc
     3961 tggtcgattc aggcgagcgg gccgggagtt gcgccgacga cgctcgaact cggccgtgaa
     4021 gacagcgccg gagccgtgca tcgtcgcctg gcccatgtcg tcgctgccta cccgatggtc
     4081 agtgcgcgcg tcggtggtcc accggcacgc atcgacgcgc gctacgcgaa cgggatcgcc
     4141 gcctcgtcgc cgtcgagttc caccgtcccg aaagcccgct gatccatgag aacagaaacc
     4201 aaagacctga tcgtcggcct cgatatcggt acatccaaag tggtcgtcgc agtggccgaa
     4261 gcgctgccgg acgggcgctt ccaggtgatt ggactggggc aggccgcgtc gcagggactg
     4321 cgcaagggcg tggttgtcaa catcgaatcg accgtccagt ccatccgtcg ggcactcgag
     4381 gaggctgaac tgatggcggc ctgccagatc aaggacgcct tcacgggcat cgccggcagc
     4441 cacatcaaga gcttcaattc gagcggcatg gtggccatca aggatcgcga agtcaccgcc
     4501 agcgacgtcg cgcgcgtgat cgagacggca aaggcgatca acatcccgac cgatcagcag
     4561 gtgctgcacg tgctgacgca ggagttcatc gtcgacggcc aggaggacat ccgcgagccg
     4621 atcggcatga gcggggtgcg actcgaggtg cgagtacaca tcgtcaccgg cgccgtcagc
     4681 gcggcgcaga acatcatcaa gtgcgtgcgt cgctgcgggc tcgaggtgca ggacctgatc
     4741 ctgcagccac tggcctcttc gctctcggtg ctgaccgccg acgagaagga actcggcgtg
     4801 gccctcgtcg atgtcggcgg cggcaccagt gacatcgcca ttttcaccgg cggtgcgatc
     4861 cgtcataccg cgatcattcc gatcgccggc gaccagatca ccaacgacat cgcgatggcg
     4921 ctgcgtacgc cgatccccga cgcagaggag atcaaagtgc gctggggcgt ggccaaggaa
     4981 gttctcgccg acccgaacga aaagatcgaa ataccgggac tcggcgaccg cggtccgcgg
     5041 ctgctgtcgc ggcaggcact ggcggccgtg atcgagccgc gtgtcgagga actcttcacg
     5101 ctgacgcagc aggtcatccg cgaatccggc tacgaggaga tgctgtcgtc gggcgtcgta
     5161 ctcaccggcg gcaccagcct gcttcccggc atgactgaac tcgccgagga cgtgttcctg
     5221 aagccggcac gcatcggctg gccgcagtac gacggttcgc tcgccgacgt tgtcaagaat
     5281 ccgcgcttcg cgaccgccat gggcctgctg gtcgaggccc actcccagcg gcagcgcggc
     5341 cgcaagatcg cgcatcaaac cggatcgttc agccagacgt tcaagaggat gaaggaatgg
     5401 atcgtaggaa atttttaggc gccgctcgtt tccgcgagag gtgtgtttga gagcgtgcaa
     5461 ggccaagcgc gttggtgcag gtgcgctttt ggccttgtgc gtttcgcatt gatgcagcaa
     5521 ccagacccag gagcaggaga acacgatgac attcgagata cttgaaaccg aaacgcgcgg
     5581 gacgatcatc aaggtggtgg gggtaggcgg cgccggcggc aacgcggtcg agcacatgat
     5641 ccgtcgcagc gtgcagggcg tggagttcat ctgtgccaac acagaccatc aggcgctggc
     5701 gcgctcgtct tcgaagaacg tgctgcagct cggcaagacc gggctgggcg ccggcagccg
     5761 gccggaggcc gggcgccagg cggccgagga ggcgcgcgac cgcatcgccg attcgcttcg
     5821 tggagcgcac atggtgttca tcaccgcggg catgggtggg ggcacgggta cgggggcagc
     5881 gccggtcgtg gccgaagtcg ccaaggagct ggggctgctg acggttgccg tagtgtcgaa
     5941 gccgttcgag ttcgaaggac ccaagcgcct gaaagtggcc gaaacgggtc tggccgaact
     6001 cgaggcctcg gttcactcgc tcatcgtcat cctcaacaac aagctcgagg aagtgatggg
     6061 cgaggatgcc gagatggccg actgtttccg cgcggccgac gatgtgctgc acaacgcctg
     6121 tgccggcatt gccgagatca tcaacgtgcc ggggctggtc aacgtcgatt tcgaggacgt
     6181 gaagaccgtg atgagcgagc gcggcaaggc gatgatggga accgcgacgg cgtctggcct
     6241 ggatcgcgca cgtattgcgg ccgagcaggc ggtcgccagc ccattgctcg agggcgtcga
     6301 tctgtccggc gcgcgcggcg tgctggtcaa catcaccgca tcgtcatcgc tgaagatgaa
     6361 ggaaacgcgc gaggtgatga acacgatccg tgcgtttgcc gcggatgacg cgaccgtgat
     6421 cttcggcacc gtctgtgacg aatcgatcgg cgacggtctg cgggtcaccg tggtggcgac
     6481 cggcctcggg cgggcgggct cgaagaagca gtccaatccg ttcacggtcg tgcgcaccgg
     6541 caccgacaac gcacccttga tggttgcgcc gacccgcgag actgcgacgg ccgggtccga
     6601 ttacagtcac ctcgacaccc ccgccgtctg gcgctcacgc gaatctgcgc agcagaaggt
     6661 ctcggcaatg gaggaaagcg gcagcgaccg ctacgacatt ccggcgtttc tgcggaaaca
     6721 ggcggattga ggggggcgcc gagggtgtac gcgatcgctg acgcgatcgc acgtgcaccc
     6781 tcggcggcac gcgcacgacg tgcgcgctca agactggtca gtcggctgcg ctccggagcg
     6841 gccgcgcgct gcgcctaagg gcaaaggcgg acaccccact cccggaagca gcgtgcttgt
     6901 tgctgtttcg tcattgccct ccccgccagg aggggagggt gagggagggg tgcacttctc
     6961 ctcccgctcg gcgggaggtc gggtgggtgc ggtgcagttg aaactcggcg aagcgcgcgg
     7021 ccgctccgga gcgcagccga cagttgttag ccgtcgtcga tgcacgtgcg atccgaagga
     7081 tcgcgaccat cgacgacgag atctctgtag gcatgccacg tcgcgtgccc aattactggc
     7141 acggcgacga cgaggccgac caag
//
LOCUS       DATIRT010000350         2149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_841841, whole genome shotgun sequence.
ACCESSION   DATIRT010000350 DATIRT010000000
VERSION     DATIRT010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841841"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1272
                     /locus_tag="VLN25_07955"
     CDS             <1..1272
                     /locus_tag="VLN25_07955"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HSQ10534.1"
                     /translation="AFSAIQKDVKLFYAMVLLIQFTMLGTFLAQDLFLFYVFWEAMLI
                     PMFLMIGIWGGERRIYATLKFVLYTAFGSILMLAAVIYLVYSLQQSTGTLSFSFADLY
                     RHRLPVQVQGWLLAAFALSFAIKVPIVPLHTWLPDAHVEAPTAGSVILAGVLLKMGTY
                     GFIKLGFPLFPQATAAAVNWVMLAAVISIVYGACLALVQTDIKKIIAYSSISHLGYVM
                     LGLASLDLIGIQGAIIQMVSHGLTAAGLFLMVGMIYERCHTRELAAYGGLAKVLPVYS
                     VFFMILTLAAIGLPTTSGFTGEFLVLLGAFNAAWPLYQTGDSVPLVLAVVAVTGVVLG
                     AMYMLWFAQRFLYGAVKVPHAPITDLNGRERAILVALVVAVFWLGLFPQEAMRKTETA
                     ARQYQQWVDVAGQGSGATAQARAALQPEAAR"
     gene            1269..>2149
                     /locus_tag="VLN25_07960"
     CDS             1269..>2149
                     /locus_tag="VLN25_07960"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HSQ10535.1"
                     /translation="MNWSVMLMAMLPEHLLLAGIVVLISMEVAGISSRGSASVALLFT
                     LLSTLAAALLALSGFEGAPFPGHFSVDPPTMMAKAIVLALAIPIVQMSRSEFASDVRF
                     YILLLSSLYGVLLVTSSDGFLTMFLGLEIMSMPVYVLVLLAYQRPEAAEAALKYLVLG
                     GAASAMFLMGASLVYGATGSLALDAFVQAAQAKDALAVVAVVLVMVAFFLKAAVVPFH
                     AWAPDAYEAASVPVTAYMATLIKAGVLLAVVRILGTAQISSPLVDLLAILPLISIVWG
                     NLAAMRQPSFRRMIAYSS"
BASE COUNT          299 a          686 c          702 g          462 t
ORIGIN      
        1 gcgttcagcg cgatccagaa ggacgtgaag ctcttctacg ctatggtgct cctgatccag
       61 ttcacgatgc tcggtacctt ccttgcgcag gatctcttcc tgttctacgt cttctgggag
      121 gcgatgctga tcccgatgtt cctgatgatc ggtatctggg gcggcgagcg gcgcatctac
      181 gcgacgctga agttcgtgct gtacacggcg ttcggctcga tcctgatgct cgccgcggtc
      241 atctatctcg tgtattcgct tcagcagagc accggcacgc tgtcgttctc gttcgccgat
      301 ctgtaccggc accggctacc ggtccaggtg cagggctggc tgctggccgc gttcgcgctg
      361 tcattcgcga tcaaggtgcc gattgtgccg ctgcacacgt ggttgcccga tgcgcacgtc
      421 gaggcgccca ccgcaggctc ggtgatcctg gccggcgtgc tgctgaagat gggaacctac
      481 ggtttcatca agctcgggtt cccgctgttt ccgcaggcga cggctgccgc ggtcaactgg
      541 gtaatgctgg cggccgtcat cagcatcgtc tacggcgcct gtctggcgct ggtgcagacc
      601 gacatcaaga agatcattgc gtactcatcc atcagccacc tcggctatgt gatgctcggt
      661 ctcgcgagcc tggacctcat cggcattcag ggcgcgatca tccagatggt cagccacgga
      721 ctgacggcgg ccggactgtt tcttatggtc gggatgatct acgaacgctg ccacacgcgc
      781 gaactcgcgg cctacggcgg cctagcgaag gtgctgccgg tgtactcggt gttcttcatg
      841 atcctgacgc tggccgcgat cggcctgccg acaaccagcg gcttcaccgg cgaattcctg
      901 gtgctgctcg gcgcattcaa cgcggcctgg ccgctctacc agaccggtga ctcggtgcca
      961 ctggtgctgg cagtggttgc agtgaccgga gtcgtgctgg gagcgatgta catgctgtgg
     1021 tttgcgcagc gcttcctcta tggcgccgtc aaggtgccgc acgcgccgat caccgatctc
     1081 aacggccgcg agcgggcgat cctggtagct ctcgttgtcg ctgtcttctg gctgggtctc
     1141 ttcccgcagg aggcgatgcg caagaccgaa acggcggcac ggcagtatca gcagtgggtc
     1201 gacgttgcag gccagggatc cggcgccacc gcgcaggcgc gcgccgcgct gcaaccggag
     1261 gccgcgcgat gaactggtcc gtcatgctca tggcgatgct tcccgagcac ctgctgctcg
     1321 ccggcatagt cgtactgatc tccatggaag tcgccggcat ctcgtcgcgc gggtctgcgt
     1381 cggtggcgct gctgttcacg ctgctttcga cgctggccgc cgcgctgctg gcgctgtcgg
     1441 gattcgaagg cgcaccgttt ccggggcact tctcggtcga tccgccgacg atgatggcaa
     1501 aagcgatcgt gctggcactg gcgatcccga ttgtccagat gtcgcgcagc gaattcgctt
     1561 cggatgtccg cttctacatc ctgctgttgt cgtcgctcta tggcgtcctg ctggtgacct
     1621 cgtctgacgg cttcctgaca atgttcctcg ggctggagat catgtcgatg ccggtctacg
     1681 tgctggtact gctggcgtac cagcggcccg aggcggccga ggcggcgctg aagtacctgg
     1741 tgctcggagg cgccgcgtcg gccatgttcc tgatgggtgc ctcgctcgtc tacggcgcga
     1801 ccggatcgct cgccctcgac gcttttgtgc aggcggcgca ggccaaggat gcgctcgcgg
     1861 tggtggccgt cgtgctggtg atggtggcgt tcttcctgaa ggccgcggtg gttcccttcc
     1921 acgcatgggc gcccgacgcc tacgaggccg ccagcgttcc ggtaaccgcc tacatggcca
     1981 cgctgatcaa ggccggcgtg ctgctcgccg tcgtgcggat cctcggcacg gcgcagatca
     2041 gcagtccgct ggtcgacctg ctcgcgatcc tgccgctgat ctcgatcgtg tggggcaacc
     2101 tggccgcgat gcggcagccg agcttccgcc gcatgatcgc gtactcgtc
//
LOCUS       DATIRT010000351         1842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1038434, whole genome shotgun sequence.
ACCESSION   DATIRT010000351 DATIRT010000000
VERSION     DATIRT010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038434"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1824)
                     /locus_tag="VLN25_07965"
     CDS             complement(<1..1824)
                     /locus_tag="VLN25_07965"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSQ10536.1"
                     /translation="MAARSDLITAMTRIDAEEAVRQWAQRSRRLGGDSAIEIYSFQGE
                     LVERIGDADGFRLAEPSDADEVRRALTGEDTLSVVERLQGLTLRAVVPVRSGSRIVGA
                     VGAERLIDAAYIGELATRLGLDVGLVARGRVLVGTAHADDRRCLEKIADRLANGAAGS
                     IALDGNLDVAVRPLSLSREPVAVAALMPNAQAYTALSGSLNAFVTVVLFTIFATILAG
                     VYLTRYLIQPVKALTERAEELSLRFAGRRVARRGDELDSLVGSFEAMTTALLSHSDRL
                     ARAHKSELQNSLELQHQYAQMRLLRGLAAAANEGGTVEATLERALHEIGGYLDWPLGR
                     VALQQESIADAVLPPRSIWFVRDVERFGSFIDASNRTPIVPSPDHLIGRAYLSGTPHW
                     VSDLSRMSNWNRLVEALDTGLQTGIVIPVTARGHVTAFIEFFCDHRVEATNELLELLE
                     AISAELSRVAERQRAERDLRERELESSRLAMVASRTDQMVMILDSSGRIEWANDACAR
                     FSGYSLQDVRGRLAHTLLQGPQTDAAATTAIAEAVVRGEPCRVEFVAYTRDGEMRVLE
                     VEGQPLRDDQGRYFQYALISPDITERKRTEAALRESAEYFRA"
BASE COUNT          320 a          629 c          592 g          301 t
ORIGIN      
        1 tgcgcggaaa tactcggcgc tttcgcgcag cgccgcctcg gtgcgcttgc gctcggtgat
       61 gtccgggctg atcaacgcat actggaaata gcgcccctgg tcgtcacgca gcggctgccc
      121 ttccacttca agcacgcgca tctctccgtc gcgcgtgtag gcgacgaatt cgacccggca
      181 cggctcgccg cgaacgacgg cctcggcgat ggccgtcgtc gccgcggcgt cggtctgcgg
      241 accctgcaaa agcgtatggg ccaggcggcc acggacatcc tgcaacgagt atccggaaaa
      301 gcgcgcacag gcatcgttgg cccattcgat gcgccccgag gagtcgagga tcatcaccat
      361 ctggtcggtg cgcgatgcca ccatggccag ccggctcgat tccagttcgc gttcgcgcag
      421 atcgcgttcg gcgcgctgcc gttcggccac gcgcgagagc tccgcgctga tcgcctcgag
      481 cagttcgagc agttcgttgg tggcttcgac gcggtgatcg cagaagaact cgatgaacgc
      541 ggtcacgtgg ccgcgtgcgg tcaccggaat cacgatgcca gtctgcagcc ccgtgtcgag
      601 cgcctcgacc agccggttcc agttcgacat gcgcgacaga tcggagaccc agtgcggcgt
      661 gccggacagg taggcacgac cgatcaggtg gtccggggac ggcacgatcg gcgtccgatt
      721 gctggcatcg atgaacgacc cgaagcgctc gacgtcccgc acgaaccaga tcgatcgcgg
      781 aggcaagacg gcgtctgcga tgctttcctg ttgcaaagcc acccgtccga gcggccagtc
      841 caggtagccg ccgatctcgt gcagcgcgcg ctcgagcgtc gcttcgacgg ttcccccctc
      901 gttggccgcc gctgccagcc cacgcagcag gcgcatctgc gcgtactggt gctgcaactc
      961 gaggctgttc tgaagttcgc tcttgtgggc tcgcgccagc cggtcagagt gagacagcag
     1021 cgctgtcgtc atggcctcga aggagcccac cagcgagtca agttcatcgc cacgccgcgc
     1081 cactcgccgt ccggcgaagc gcagcgacag ttcctcggcg cgctcggtga gcgctttcac
     1141 cggctgaatc aggtagcgcg tcaagtagac accggccagg atggtggcaa agatcgtgaa
     1201 caacacgact gtcacgaacg cattgagcga tccgctcagg gccgtatacg cctgcgcatt
     1261 cggcatcaac gcggcgaccg cgaccggctc gcgcgaaagc gacagcggac gcaccgctac
     1321 gtcaaggttg ccgtcgagcg cgatcgaacc ggcggcgccg ttagccagcc ggtcggcaat
     1381 cttctccaga cagcggcgat cgtctgcgtg cgcggtgccg acgagtaccc gtccacgtgc
     1441 gacaagaccg acgtccagtc cgagacgggt ggccagttcg ccgatgtaag cggcatcgat
     1501 caggcgttcg gctccgacgg cgccgacgat gcggctgccc gatcgcaccg ggaccacggc
     1561 tcgcagtgtc agaccctgaa gccgctcgac gactgaaagc gtgtcctcgc cggtgagggc
     1621 tcggcgcacc tcatcagcgt ccgacggctc ggcaaggcgg aagccatccg cgtccccgat
     1681 ccgttcgacc agttcacctt gaaaactgta gatctcgatc gccgaatcgc ccccaaggcg
     1741 ccgagagcgc tgcgcccatt gacgcacggc ctcttcggca tcgatgcgcg tcattgccgt
     1801 gatcagatcg gatcgtgccg ccatggcgcg cgcagtctgc tc
//
LOCUS       DATIRT010000352         1750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_54851, whole genome shotgun sequence.
ACCESSION   DATIRT010000352 DATIRT010000000
VERSION     DATIRT010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54851"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1394)
                     /locus_tag="VLN25_07970"
     CDS             complement(<1..1394)
                     /locus_tag="VLN25_07970"
                     /inference="COORDINATES: protein motif:HMM:NF014398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HSQ10537.1"
                     /translation="MLRVLQATAVLGVFAAPFASAQQAPAKTSATPKVAPAAKPAKVD
                     ASGCFGCHAPIKEFHDSGKHKGLACSSCHSGIEKHLGDPKSRPVTSTDPATCGGCHKN
                     QFDTAYQMDWHRTGRFEKKQMTGPSPNPAFDQLMTPHGFTREHNLPRSHAFMVLDQFV
                     VDRGFGGRFTPKDSWRYLAQPGGNFKVWDVIQDNYPDNTDQKVFKPGTAAAATPVCIS
                     CKTQDHILDWAYMGDPVPGAKWSRSSKVVEVVKSVNHANNCIFCHDPHAAKPRIVRDA
                     LIQALTRKDFPTLYSEDPRKTKFEVKDMGVRGFNRKIAMLESYDSKLQCAQCHVEYNC
                     NPGVDPKTGAAIGMADQRTNVFPLVAVSNITKFYDNINFRDFRHFQTGALLTKMQHPD
                     AEVFWNSKHDKAGVQCQSCHMPKVTDPKTKKTFTSHWQTNPKQYIKQTCLTCHTDWSE
                     QQARYVMESMASHYA"
     gene            complement(1546..>1750)
                     /locus_tag="VLN25_07975"
     CDS             complement(1546..>1750)
                     /locus_tag="VLN25_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HSQ10538.1"
                     /translation="LPARFTLDDTMAMSQDMKLSTTPSVIVGARVSKSGRPMPSSGDL
                     EGLSKPVAIGTKDVAVTIDRVLP"
BASE COUNT          279 a          524 c          579 g          368 t
ORIGIN      
        1 gcgtagtgcg aagccatcga ttccattacg tacctggcct gctgctcgct ccagtccgtg
       61 tggcaggtca ggcaggtctg cttgatgtac tgcttcggat tcgtctgcca gtgcgacgtg
      121 aaggtcttct tggtcttcgg atccgtgact ttcggcatgt ggcacgactg gcactgcact
      181 ccggccttgt catgcttcga gttccagaac acttccgcgt cggggtgctg catcttggtc
      241 agcaacgcgc ccgtctggaa gtggcggaag tcgcggaagt tgatgttgtc gtagaacttg
      301 gtgatgttgc tgacggcgac cagcgggaac acgttggtgc gctggtcggc catgccgatc
      361 gccgcgccgg tcttggggtc aacgcccggg ttgcagttgt actcgacgtg gcactgcgcg
      421 cactgcagct tgctgtcgta gctttcgagc atcgcgatct tgcggttgaa tccccgcacg
      481 cccatgtcct tgacctcgaa cttggtcttg cgcgggtctt ccgagtacag cgtcgggaag
      541 tccttgcgtg tcagtgcctg gatcagcgca tcgcgcacga tgcgaggctt ggccgcatgc
      601 gggtcgtggc agaagatgca gttgttggcg tggttgaccg acttgacgac ctcgaccacc
      661 ttggatgacc ggctccactt ggctcccggt accggatcgc ccatgtaggc ccagtcgaga
      721 atgtggtcct gcgtcttgca ggaaatgcac accggagtgg ccgccgcggc cgtgcccggt
      781 ttgaacacct tctggtcggt gttgtcgggg tagttgtcct ggatgacgtc ccagaccttg
      841 aagttgccgc cgggctgggc gaggtagcgc cagctgtcct tgggcgtaaa ccgtcctccg
      901 aagccacgat cgaccacgaa ctggtcgagc accatgaagg catgcgagcg cggcaggttg
      961 tgctcgcgcg tgaagccgtg cggggtcatc agctgatcga acgccgggtt gggcgacggc
     1021 ccggtcatct gcttcttctc gaagcggccg gtccggtgcc agtccatctg gtaggcggtg
     1081 tcgaactggt tcttgtggca gccgccacag gtggcaggat cggtcgaggt cactggccgc
     1141 gacttgggat cgccgagatg cttctcgatg ccgctgtggc acgaggagca ggccagtccc
     1201 ttgtgcttgc cggagtcgtg gaattccttg atcggcgcgt ggcagccgaa gcaacccgag
     1261 gcatcgacct tggccggctt ggccgccggt gcgaccttcg gtgttgccga agtcttggcc
     1321 ggcgcctgct gggccgacgc gaacggcgcg gcgaatacac ccagtactgc agtcgcctgc
     1381 aaaactcgca gcaaacttgt tctcatcccc atggtgcccc cacctcgcta gtaaacgaat
     1441 cggggcgacg gtaacaaccg ggacaagcgc cgcacttgac tgccatcaag gcggtcgagc
     1501 ccacgataga agctacctcg tcggaggtag ccggggccgg gcggatcagg gaaggacgcg
     1561 atcgatggtg accgccacgt ccttcgttcc gatcgcgacg ggcttcgaca gcccctcgag
     1621 gtcgccactg gagggcatcg gccggcctga cttcgacact cgtgctccga ctatcacgga
     1681 gggcgtcgtc gacagtttca tgtcctgcga catcgccatc gtgtcgtcga gcgtgaacct
     1741 ggctggtagc
//
LOCUS       DATIRT010000353         8343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_940735, whole genome shotgun sequence.
ACCESSION   DATIRT010000353 DATIRT010000000
VERSION     DATIRT010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8343
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940735"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VLN25_07980"
     CDS             <1..280
                     /locus_tag="VLN25_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10539.1"
                     /translation="RLVGPLELAGLVARVEGRKARGTTMRRDARTRPLQLTAKGERRL
                     RAATPAWQDVQSRVDGLLGTRLQATLTQAADAAGRALAAVDIESADRS"
     gene            277..1503
                     /locus_tag="VLN25_07985"
     CDS             277..1503
                     /locus_tag="VLN25_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSQ10540.1"
                     /translation="MSGNPQRTWWGVTLIAAAMLMVTMGARQSMGLFVSPINTTTGLG
                     IVMISLALGIGQFVWGAVQPVAGAFADRYGPGRVLAVGIVVLAIGTALTPLVSSGFGL
                     VLTLGLMAAAGSGAGSFSVLIGAAAKRVPAESRGTASGVINAGGSFGQFIFAPLLQKL
                     ISLMGWMGAMWALAFITLATLPLARALRGQPAASHAPAIPGDHGLRHAVRSALGDRSY
                     LLLHAGFFTCGFHIAFLVTHLPGEVGLCGLPSSVASWSLAIIGLANIAGSLIAGFWVN
                     RFRSKYILFWMYLSRAVLVVACLAAPKTALTFYLFAAGLGFTWLATVPPTAGVVAKLF
                     GTRYLATLFGLTLLSHQIGGFFGAYLGGIAVSTQGSYLWMWYADAVLALAAALVNLPI
                     REPRVERRPVVAATSP"
     gene            complement(1553..2446)
                     /locus_tag="VLN25_07990"
     CDS             complement(1553..2446)
                     /locus_tag="VLN25_07990"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HSQ10541.1"
                     /translation="MKHRIHPLIATLIACLGIAPLPEAAAIRIDPTPAALAGIMAERR
                     SVLLGEVHDNGEQHALRMAALRQRIEAGARPALVFEQFDRERQADIDRVRQERPGDAD
                     ALIAAVGAKNWAWEHYRPFVQIALDHGLPIVAANLSRADAMKVAASGWDGVFDASTQT
                     TLGLDRLPAEFVAAHERAVARGHCDLLPASALAPMARAQIARDIGLARSLRPYLAEGA
                     VLLSGNGHVRKDIGVPFWLSADERRDVVSIGLLERDSGKAIADASEHSEQFDVVIETA
                     AAERPDPCAALRKRLQPDARP"
     gene            complement(2524..3384)
                     /locus_tag="VLN25_07995"
     CDS             complement(2524..3384)
                     /locus_tag="VLN25_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSQ10542.1"
                     /translation="MKTVRLAGRDGVSVPTLGLGTWRLGESSRRRAAEVAALRRAFEL
                     GYRLIDTAEMYGEGGAEQIVGQALAEAIAAGELRRDDVFIVTKVTPHNASARGVAQAC
                     DRSRTRLRIDAVDLYLLHWRGSHPLTETITGFEALCDAGRIRAWGVSNFDVDDMQQLW
                     RQPAGTRCAVNQVYYSASQRGIEFDLLPWQRERSVATISYCPIDQGTLAADAGLAQIA
                     RRHGVTAAQVALAWVLRHPDVIAIPKASREDHQRENLAAASITLDVDDCTEIDRLFAA
                     PLSKQPLAMT"
     gene            complement(3423..3920)
                     /locus_tag="VLN25_08000"
     CDS             complement(3423..3920)
                     /locus_tag="VLN25_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10543.1"
                     /translation="MLDAQTWEIASYVVTVIGLPMAIALFLFEQRKEHSTEEDEVYQL
                     LSNAYNDFLKVVMDNPDLRLRSELATLDLSPEQQERMLIVFDMLISLFERAYLTAYSE
                     SMTPLQRRRWNSWEDFMREWVRRDDFYHRLPKLLKGEDPEFAAYLQRIAEDERGKPLP
                     RLPLS"
     gene            complement(3931..4620)
                     /locus_tag="VLN25_08005"
     CDS             complement(3931..4620)
                     /locus_tag="VLN25_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008159766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HSQ10544.1"
                     /translation="MAEPDPLRLADMLAASAWWPALQPAERERMLAEVEERRYGGGSV
                     VMRKGELVDAWTGVVDGLVKINVVSPSGKTATFTGVGPGGWFGEGSLIKNEARKYDVV
                     ALRDTRIAWMPRRTFERLMDTSIAFNRFLLTQLNERLGQFIGMVEYERLLEPDARVAR
                     CLAELFNPHLYPGNRAELEISQEEIGYLSGVSRQRVNQALQVLARAGLLEVAYGRVRI
                     LDMTTLRRYGD"
     gene            4804..6753
                     /locus_tag="VLN25_08010"
     CDS             4804..6753
                     /locus_tag="VLN25_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSQ10545.1"
                     /translation="MAEIVTADTFPRLLKLHAQQRPGSAAIREKDLGIWQSWSWAQTL
                     TEVRQIAAGLHQAGFRRGEHLAIIGENRPRLYFTMIAAQCLGGIPVPMYQDAVAPEMA
                     FVFDNAEIKFAVVEDQEQVDKLLEIRETVPHLKRIIYEDPRGLRNYDQPGLASYEQIR
                     ALGEQLLKERPAIVDDEIDKGRANDVAAMFYTSGTTGNPKGVVLEFSNLLAAGRAGAD
                     MESLSHREEVLAYLPMAWVGQNIFSYTQWLVCGFTVNCPESAATVMADLREIGPTYYF
                     APPRVLEALLTQVMIRMEDASAIKRRVFHHFMDVARRVGGRLLDAQPVGLGDRLAYAL
                     GDWLVYAPLRNALGMSRIRVAYTAGEAIGPDLFTFYRSLGINLKQLYGSTETSVFVCV
                     QPNGGVKPDTVGPPVRGVEIRLTDAGEILIRSPGLFREYYKSPEATREAKDAEGWFHT
                     GDAGYFDTAGHLKIIDRAKDVGRLADGTLFAPKYIENKLKFFPTIKEAVAFGHARDRV
                     CAFINIDMEAVGNWAERRNLPYSGYTDLAVKPEVVELVREAVEKVNADLANDPKLANA
                     QIRRFIVLHKELDADDEELTRTRKVRRGFIQDKYRELVDAMYAGRHEQFIETMVKFED
                     GRTGKIAATLKVCDAKSYEPMRKAA"
     gene            6759..7022
                     /locus_tag="VLN25_08015"
     CDS             6759..7022
                     /locus_tag="VLN25_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10546.1"
                     /translation="MSGRVWIAIACLVIGVPVAAWGLANGSLLLALVGLAFVLVFVFL
                     VIEAMNQAAKPKHEIPKSDNPAWSMKGDAPAASNVDHREGGSL"
     gene            7019..7804
                     /locus_tag="VLN25_08020"
     CDS             7019..7804
                     /locus_tag="VLN25_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSQ10547.1"
                     /translation="MTRQTVGEILLNIDDVWLSFGGVKAIQGVSFDVREHEIRAIIGP
                     NGAGKSSMLNVINGVYHPQQGRITFRGKVRTSMDPHDAAAEGFGRTFQSLALFKGMSV
                     LDNIMTGRNLRMRTGLLMQALHLGLARREELRHREFVEHIIDFLEIQAYRKTPVGRLP
                     YGLQKRVDLARALALEPKILLLDEPMAGMNLEEKQDMSRFVLDVNEEFGTTIVLIEHD
                     MGVVMDISDRVVVLDYGKKIGDGTPDEVRASKQVIDAYLGVAH"
     gene            7899..>8343
                     /locus_tag="VLN25_08025"
     CDS             7899..>8343
                     /locus_tag="VLN25_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSQ10548.1"
                     /translation="MSAPGFYVEVLLGGLMSGVMYSLVALGFVLIFKASGVFNFAQGA
                     MVLFAALSVARISEVMPLPFSFILAVGIMAVLAWLIERLVLRHLVNQDAVVLLMATLG
                     IAYFLDGFGQTLWGSDIYKIDTGLPKTPLLVLEQIFDGGILINTED"
BASE COUNT         1400 a         2705 c         2751 g         1487 t
ORIGIN      
        1 ccgcctggta ggcccactcg agcttgccgg actggtcgcg cgcgtggaag gacgcaaggc
       61 ccgcggcacg acgatgcgcc gcgatgcacg aacgcggccg ctgcaactga cagcaaaggg
      121 cgagagacgc ctgcgtgcgg cgacgccggc ctggcaggac gtgcaaagtc gggtggacgg
      181 cctgctcggc acacgcctgc aggccaccct gacgcaggca gccgatgccg ccggacgcgc
      241 gctggcggcg gtcgacatcg aatccgcgga ccggtcatga gcgggaaccc gcagcgcacc
      301 tggtggggcg tgacgctgat cgctgccgcc atgctgatgg tgacgatggg tgcacggcag
      361 tcgatgggat tgttcgtctc gcccatcaac accacgaccg gtctcggtat cgtgatgatc
      421 agcctcgcgt tgggtatcgg gcagttcgtc tggggcgcgg tgcagccggt cgccggcgcc
      481 tttgccgacc gatacggccc cggccgagtc ctcgccgtcg gcatcgtggt gcttgcgatc
      541 ggcaccgcgc tgacgccgct ggtgtcgagc ggcttcggtc tggttctgac gctcggactg
      601 atggcggcag cgggatcggg cgccggcagt ttctcggtgc tgatcggtgc cgcagccaaa
      661 cgtgtcccgg ctgaatcgcg cggcaccgcg tccggtgtca tcaacgccgg ggggtcgttc
      721 gggcagttca tcttcgcgcc gctgctgcag aagctgatct cgctgatggg ctggatgggt
      781 gcgatgtggg cgctggcgtt catcacgctc gcgacgctgc cgcttgcacg ggcgctgcgt
      841 gggcagccgg cggcatcgca tgcgccagcc attcccggcg accacgggct gcgccacgcg
      901 gtgcgaagtg cgctgggcga ccgcagctat ctgttgctgc acgcaggatt ttttacgtgc
      961 ggattccaca ttgcattcct ggtcacgcat ctgcccggtg aagtcgggct gtgcggactg
     1021 ccgtcatcag tcgccagctg gtcgctcgcg atcatcgggc tcgccaacat cgccggcagc
     1081 ctgattgccg gattctgggt caaccgcttt cgcagcaagt acatcctgtt ctggatgtac
     1141 ctctctcgcg cggtgctcgt cgtcgcttgc ctggcagcgc ccaagaccgc gctcaccttc
     1201 tacctcttcg ctgccgggct cggattcacc tggctggcca ccgtgccgcc gacggccggg
     1261 gtcgtggcca aactcttcgg cacgcgctat ctcgccacac tcttcggcct gacgctgctg
     1321 tcgcaccaga tcggcggctt cttcggcgcc tatctgggcg ggatcgcggt cagcacgcag
     1381 ggcagctacc tgtggatgtg gtacgcggat gccgtgctcg cgcttgcggc tgcgctggtg
     1441 aatctgccga tccgcgagcc gcgcgtcgaa cggcggccgg tggtcgccgc aacgtctccc
     1501 tgaccgctgc tgcacgggtc cgtgcgcgga atcgggacgc cagcgtccgg gttcagggtc
     1561 gcgcgtcggg ctgaagtcgc ttgcgcaggg cggcgcaggg gtcgggtcgt tctgccgccg
     1621 ccgtttcgat gacgacgtcg aactgctctg aatgctctga agcgtccgcg atcgccttcc
     1681 cggaatcgcg ctccagcaga ccgatggaca caacgtcgcg gcgctcgtcc gcgctcagcc
     1741 agaaggggac accgatgtcc ttgcgcacgt ggccattacc actgagcagt accgcgcctt
     1801 ctgcaaggta cggtcgcagt gaacgggcga gcccgatgtc gcgtgcgatc tgcgcgcgcg
     1861 ccatgggcgc cagcgccgag gccggcaaca ggtcgcagtg gccacgtgca acggcgcgtt
     1921 catgggccgc gacgaactcg gcgggcagcc ggtccaggcc gagagtcgtc tgggtcgacg
     1981 catcgaaaac cccgtcccat ccgctcgccg ccaccttcat cgcgtcggca cgcgacaggt
     2041 ttgccgcaac gatcggcagg ccatggtcga gcgcgatctg cacgaaaggc cggtagtgct
     2101 cccacgccca gttcttggca ccgaccgcgg cgatgagcgc atcggcgtcg cccggtcttt
     2161 cctgccgaac gcggtcgatg tcggcctgcc gctctcgatc gaactgttca aagacaagcg
     2221 ccggtcgcgc accggcctca atgcgctggc gcaacgccgc catgcgcagt gcgtgttgct
     2281 cgccgttgtc gtggacctcg ccgagcaaaa cgctgcgccg ctcggccatg attccggcga
     2341 gcgccgctgg cgtggggtcg atccggatgg cggctgcctc gggaagcggg gcaatgccaa
     2401 ggcaggcgat cagagtcgcg atgagcggat ggattcgatg cttcatgcaa ccattgtgcc
     2461 gggagcccac tcgggcggcc cgcgcgtcga tcccgcagag ggcctcacgc cacgccgcac
     2521 cgctcaggtc atcgcgagcg gctgcttgct cagcggcgcg gcaaacagcc ggtcgatctc
     2581 ggtgcagtcg tcgacgtcga gcgtgatcga cgcggcggcc aggttctcgc gctggtggtc
     2641 ctcgcgggag gccttcggaa ttgcgatgac atccggatga cgtagcaccc acgcgagtgc
     2701 cacctgggcc gcggtgacgc catggcgtcg cgcgatctgc gcaaggcctg cgtcggccgc
     2761 gagagtgccc tggtcgatcg ggcaatagga aatcgtggcc accgaccgct cgcgttgcca
     2821 cggcagcaga tcgaactcga tgccgcgctg gctcgccgag tagtagacct ggttgacggc
     2881 gcagcgcgtg cctgcaggtt gtcgccaaag ctgctgcatg tcgtcgacgt cgaagttcga
     2941 cacgccccag gctcgaatcc ggccggcgtc acacagcgct tcgaagcccg tgatcgtttc
     3001 ggtcagggga tggctgccgc gccagtgcag caggtacagg tcgacggcat cgatgcgcag
     3061 ccgggtccgg ctgcgatcgc aggcctgggc gaccccgcgg gcgctggcgt tgtgcggagt
     3121 caccttcgtg acgatgaaga cgtcgtcgcg ccgaagctct ccggcggcga tggcctcggc
     3181 aagcgcctgc ccgacgatct gctcggcgcc gccttccccg tacatctccg cggtgtcgat
     3241 cagccggtag cccagctcga acgctctgcg cagtgcggcg acttccgcgg cgcgacgacg
     3301 ggatgactcc ccgagccgcc acgtacccag accgagcgtc ggaacagaga cgccgtcacg
     3361 tccggccagc cgcacagtct tcatggatca gggtccttca atcaatcgat ccgcaatgcc
     3421 ggtcacgaca acggcaggcg tggcagcggt ttgccgcgct cgtcctcggc gatccgctgc
     3481 aaatacgccg cgaattccgg atcctcgccc ttcaacagct tcggcagccg atggtagaag
     3541 tcgtcgcgcc gcacccactc gcgcatgaag tcctcccacg aattccagcg gcgtcgctgc
     3601 aacggcgtca tgctctcgct gtaggccgtc aggtaggcgc gctcgaacag cgatatcagc
     3661 atgtcgaaga cgatcagcat gcgctcctgc tgctccgggc tcaggtcgag tgttgcgagc
     3721 tcgctgcgaa ggcgcaggtc cgggttgtcc atcacgacct tgagaaagtc gttgtaggcg
     3781 ttcgacagca gctggtagac ctcgtcctcc tccgtggagt gttccttgcg ctgttcgaaa
     3841 aggaagagcg caatcgccat cggcaatccg ataacggtca ccacatagct cgcgatctcc
     3901 caggtctgtg cgtcgagcac gctacgctcc ctagtcgccg taacgtcgca gcgtggtcat
     3961 gtcgaggatg cgcacgcggc cataggcgac ttcgagcagt ccggcgcgcg cgagcacctg
     4021 cagtgcctga ttgacgcgct ggcgcgagac gcccgacagg tagccgatct cctcctgcga
     4081 aatctcgagt tcggcccggt tgccggggta aagatgcgga ttgaacagtt cggccaggca
     4141 gcgcgcgaca cgcgcgtccg gctccagcag gcgctcgtac tccaccatgc cgatgaactg
     4201 gccgagccgt tcgttaagct gcgtcaggag gaatcgattg aaggcgatgc tggtgtccat
     4261 caggcgctcg aacgtccgtc gcggcatcca ggcgatgcgc gtgtcccgca gggcgacgac
     4321 gtcgtacttg cgcgcctcgt tcttgatcag ggagccctcg ccgaaccacc cgccggggcc
     4381 gacgccggtg aatgtcgcgg tcttgcccga tggcgagacg acgttgatct tcaccagacc
     4441 gtcgacaaca ccggtccagg cgtcgaccag ctcgcccttg cgcatgacca cggaaccgcc
     4501 gccgtagcgg cgttcctcga cctcggcgag catgcgctcg cgctcggccg gctgcagcgc
     4561 gggccaccag gcactggccg ccagcatgtc ggccagtcgg agcggatcgg gttcggccat
     4621 gctgtctatt ctggccgaag actgtcgccc ggacgacagc catctgtcca ccctcgccgt
     4681 accgttcgct ggcgcggtcg cggtcggcgg atttggcgcc gacagcgcgc gggcggtggg
     4741 ttaagctaac ctactacgtg ttcggtcgaa acacgaacca gaacaagccg gagacagaac
     4801 atcgtggcgg agattgtcac cgctgacacc tttcctcgcc tgttgaagct gcacgcccag
     4861 cagcggccgg gctctgcggc gatacgcgaa aaagacctcg gcatctggca gagctggagc
     4921 tgggcacaga cactgacgga agtgcgccag atcgctgccg gcctgcatca ggccggattc
     4981 cggcgcggtg agcatctggc gatcatcggc gagaaccgtc cgcgactgta tttcacgatg
     5041 attgcggccc agtgtcttgg cggcattccc gtgcccatgt accaggacgc cgtcgccccg
     5101 gagatggcat tcgttttcga caacgcggag atcaagttcg ccgtggtcga ggaccaggag
     5161 caggtcgaca aactgctcga gatccgcgag accgtgcccc acctcaagcg gatcatctat
     5221 gaggatccac gcgggctgcg caactacgac cagccgggcc tcgcgtccta cgagcagatc
     5281 cgggcgctgg gcgagcagct tctgaaggag cgcccggcga tcgtcgatga cgagatcgac
     5341 aaaggccggg ccaacgacgt cgcggcgatg ttctacacat ccgggacgac cggcaatcca
     5401 aagggtgtcg ttctggaatt cagcaacctg ctcgccgccg gccgggcggg cgcggacatg
     5461 gagagcctgt cgcatcgcga ggaagtgctg gcctatctgc cgatggcgtg ggtcgggcag
     5521 aacatctttt cctatacaca gtggctggtc tgcggcttca ccgtcaattg cccagagtcg
     5581 gctgccacgg tgatggctga cctgcgcgag atcggcccga cctactactt cgccccgccg
     5641 cgcgtgctgg aggcgttgct gacccaggtg atgatccgca tggaggatgc ctccgcgatc
     5701 aagcggcgcg tgttccacca tttcatggac gttgcccgcc gtgtcggggg ccgtctgctg
     5761 gacgcgcagc cggttggact gggcgaccgg ctcgcgtatg cactgggcga ctggctcgtc
     5821 tacgcgccat tgcgaaatgc gctcggcatg agccggatac gcgtggccta cacggccgga
     5881 gaggcgatcg gcccggatct tttcaccttc taccgctcgc tcggcatcaa cctgaagcag
     5941 ctgtacggct ccaccgagac ctcggtgttt gtctgcgtgc agccgaacgg cggcgtgaag
     6001 cccgataccg tcggacctcc ggtcaggggt gtcgagatac gcctgaccga tgccggcgaa
     6061 atcctgatcc gctccccggg cctgttccgc gagtactaca agtcgccgga agcaacgcgc
     6121 gaggccaagg atgcggaggg ctggttccat accggcgacg ccggctactt cgacactgca
     6181 ggccacctga agatcatcga tcgcgccaag gatgtcggcc gactggctga cgggacgctc
     6241 ttcgccccga agtacataga gaacaagctg aaattcttcc cgacaatcaa ggaagcggtc
     6301 gccttcggcc acgcgcgcga ccgggtctgc gccttcatca acatcgacat ggaggccgtc
     6361 ggcaactggg ccgagcggcg caacctgcct tactccggct acacggacct tgcggtcaaa
     6421 cccgaagtcg tcgaactggt ccgcgaggcg gtcgagaagg tcaatgcgga tctcgccaat
     6481 gatccgaagc tggcgaacgc ccagatccgg cgcttcatcg tgttgcacaa ggaactcgat
     6541 gccgacgacg aagagttgac tcgcacgcgc aaggtgcggc gcggcttcat tcaggacaaa
     6601 taccgcgaac tggtcgatgc gatgtacgcc ggccgccacg aacagttcat cgagacgatg
     6661 gtgaagttcg aggacggacg cacgggcaag atcgcggcca cgctcaaggt gtgcgacgcc
     6721 aagagctacg agccgatgcg aaaggcggcc tgagcgtcat gagtggacgc gtctggatcg
     6781 ccatcgcctg cctcgtcatc ggcgttcctg tggcggcctg ggggctcgcc aacggttcgc
     6841 tgttgctggc tctcgtcggc ctggccttcg tgctcgtgtt tgtcttcctc gtaatcgagg
     6901 caatgaatca ggcggccaag cccaagcatg agatcccgaa gtcggacaac ccggcgtgga
     6961 gcatgaaggg tgatgcgccg gcggcatcga acgtcgatca tcgcgaaggc ggttcgctgt
     7021 gacgcgccag accgtcggcg agatcctgct gaacatcgac gacgtgtggt tatcgttcgg
     7081 gggcgtgaaa gcgatccagg gcgtgtcctt cgatgtccgc gagcacgaga tccgcgccat
     7141 catcggaccg aacggcgccg gcaagagttc gatgctcaac gtcatcaacg gcgtctacca
     7201 cccgcagcag ggccgcataa cgtttcgcgg caaggtgcgc acgtcgatgg atccgcacga
     7261 cgcggccgcc gaagggttcg gacgcacgtt ccagagcctt gccctgttca agggcatgtc
     7321 ggtgctcgac aacatcatga ccggtcgcaa tctgcggatg cgcaccgggc tgctgatgca
     7381 ggccctgcac ctcggcctgg cgcgacgcga ggaactgcgg catcgcgaat tcgtcgagca
     7441 catcattgat tttctcgaga tccaggccta tcgcaagaca ccggtcggcc ggttgcctta
     7501 cgggctgcag aagcgcgtcg acctggcgcg cgcacttgca ctggaaccga agatcctgct
     7561 gcttgacgag cccatggcgg gcatgaacct cgaggaaaag caggacatgt cgcgcttcgt
     7621 gctcgacgtg aacgaggagt tcggcaccac catcgtgctg atcgagcacg acatgggggt
     7681 cgtgatggac atctccgacc gtgttgtagt gctcgattac ggcaagaaga tcggcgacgg
     7741 cacgccggac gaagtcaggg ccagcaagca ggtcatcgac gcctacctag gcgtggcgca
     7801 ctgaggcaac gatggttcag accctgacga agaactggcc ggtggtcctg ctgctcttcc
     7861 tgatgagtat cgggctggtc gctccgctgg cgctcggcat gtcggcaccc ggcttttacg
     7921 tcgaagtgct gctcggtggt ctgatgtcgg gcgtcatgta ttcgctggtc gcgctcggct
     7981 tcgtcctgat tttcaaggcg tcgggcgtct tcaattttgc ccagggagcg atggttctct
     8041 ttgcggccct gtcggtggca cgtatcagtg aagtgatgcc gctgcccttc tccttcatcc
     8101 tcgctgtcgg catcatggcc gtgctcgcct ggctaatcga acggctggtg cttcgccatc
     8161 tcgtcaacca ggacgccgtc gttcttctga tggccaccct cggcattgcg tacttcctcg
     8221 acggcttcgg gcagaccctt tggggcagcg atatctacaa gatcgacacc gggctgccca
     8281 agacgccgtt gctggtgctg gagcagatct tcgatggcgg catcctgatc aacaccgaag
     8341 aca
//
LOCUS       DATIRT010000354         6863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_843068, whole genome shotgun sequence.
ACCESSION   DATIRT010000354 DATIRT010000000
VERSION     DATIRT010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6863
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843068"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..244)
                     /gene="ffs"
                     /locus_tag="VLN25_08030"
     ncRNA           complement(146..244)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VLN25_08030"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(285..974)
                     /locus_tag="VLN25_08035"
     CDS             complement(285..974)
                     /locus_tag="VLN25_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10549.1"
                     /translation="MHSTLRIAGLATVGLTALWGCQSAPPQLNPKDMTFFVTSVGPGK
                     GADLGGLEGADQHCAKLAKAAGALDRTWRAYLSTQAPGLRDPNFVNARDRIGTGPWQN
                     VMGVTVATSVDDLHSPASNLAKQTALDERGQVVNGTVDKPNRHDILTGSRPDGTAFPG
                     TPFPDMTCGNWTKGGADGSAMVGHHDRAGPMMTSTWTTSWNSSHPTLGCDMAKLNSTG
                     GDGRLYCFAVK"
     gene            complement(1080..4385)
                     /locus_tag="VLN25_08040"
     CDS             complement(1080..4385)
                     /locus_tag="VLN25_08040"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HSQ10550.1"
                     /translation="MSDYLADEIPIDEDDFTRRACDPRSSVVVEACAGSGKTWLLVSR
                     IVRLLLAGTAPGEILAITFTRRAAQEMKVRLLRDLRDLASATDDKIVASLRQRGLSRA
                     QAVAAIDDARRLYERVVTARVPLTIETFHGWFWQLIARAPLGAGVPFAPVLLEGSERV
                     RADAWLHFTAALIRPERAAERAAWEDLIGETGEVTARRLLQQFLHKRAEWWSFAAGDQ
                     PAAVDRALEPLRVSGGRDPAMELRAPDFTGSLRGLVAQWQSIRPPLGTIDMAVGRASA
                     WLASPGKNPARDLRVACLVVLTKGRSTPIVLLTPECMAARFANDTQARSYANAHAAVV
                     VRLQQIMDARRTWRAFKVNHAALTCGQLLVDTYQALKQQRQALDFTDLEWHTYRLLSD
                     PDNAAYMHARLDARYRHVLLDEFQDTNSLQWQVLQSWLAAYGVQDVGAGSGSSQDRPT
                     VFVVGDPKQSIYRFRRAEPRVFDAALELLCRDYAACHLRTNVTRRNAPAVIEVLNRAM
                     PRGNPLYQPQSTRAPYSIGQGAFVLLPLEAPEPATEMAGDGISLRDVLTTPRAERETD
                     TRYREGRVLANEIAAWKLRLQVVDDDVLRPARWSDVLVLVRRRTHLADYERALRDAGI
                     PFISDRRGGLLATLEAADLTALLSFLTAPYSDLQLAHALRSPIGGCSDDDLMRLAAAS
                     GASWWDRLQQLPDPVSAPLARARELFLWWLDLAGVLPVHDLLDRIYFEGDLRRRYAAA
                     VPDALHAQVQANLDAFIELALAIDAGRYPSLPRFIDELAGLRHHATDEAPDEGPTGAE
                     DAVRVMTIHGAKGLEAEIVALADAHVRAPLEGEGVLVDWPPQEPAPEHFSLVTRGEEA
                     RDLARRDWFERDEEQRRQEDWNLLYVAATRARQVLIVSGSAPSRGSLDDTWYTRLQCA
                     EDLSSGAAAAVALAPPLPVRILRDFLPDPLPTGGRREAAGESDAMRLGRAWHALLEHG
                     SDAPVDAIARAHALTQEQRDTVVAAAAQVRAVHWGLFDARDVAELELVADDGELLRVD
                     RLVERDDALWIIDFKWQVTDAERPAYEAQVRRYADVLRSIRGDRPVRMGLVTAAGELI
                     EVPSGVSIRVDER"
     gene            complement(4382..>6863)
                     /locus_tag="VLN25_08045"
     CDS             complement(4382..>6863)
                     /locus_tag="VLN25_08045"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HSQ10551.1"
                     /translation="TLWALARDVAHLCDELTLASVSGAEDFDGRLQTSLRRHFHRRAA
                     HALQAQSQLVIQLWRARREADDGAARFLRELAARASRTQLPLVYLGTGLSTAGRAGLA
                     TWERAFIDRAAEQAPVLLLDPDVTAALEDQPLLAAAWPELGGVDTDVAIAVRADAIPA
                     APRPSTPLSIVKALTLEEEAIAVAQQVVEWRRAGVGSIALVALDRLTARRVRALLERA
                     QVSVRDETGWKLSTTSAAAAIMRWYDVVADDLYWRDLLDWLKSNFTLSGRPDKSQEVD
                     RIERAIRAAGALQGAKSIRRALANHGSGEAREHGVGALEVIALVESQAQATRRAAPTL
                     HSHVQALRQAMDALGMRAALTADPVGQAVLIDLAALEAELATLGGRASLADFRALLAS
                     RFEEVAFVDRRIESPVVMVSLASAALRSFDAAVLVGADAQHLPSRPSEVLFMSNAVRA
                     ELGLTTAESAVLDQAAQLATLLATATQVVATWRTCRGDDPSPLSPLLDRLRFIAQRVL
                     GDDLTRPALPEYVDVEVAVTTRPAPRASQLLPARISASQAQSLVDCPYQFYARRMLGL
                     RAPDDVIELPEKREFGIALHEVLSRFHKTWGAVDFSGVAPVELVASLREHARAVFGPQ
                     IERTPGLLAFERRFDGLVDGYIDWLQQHAGNGWLWTGGEENHRRPIVLNAGHAVELIG
                     RVDRVDTRADGRLRLLDYKARAADVLKRGLKSPGEDIQLPFYGMLLPRRPGSGEYVSF
                     DRVREDTSGVKSVAPTGAFDELIDVVEARLTADLQRMVDGVAMPAIGAESVCVNCEMR
                     GLCRRDYWKHSGEHGGESGDPDSGDPFA"
BASE COUNT         1149 a         2368 c         2247 g         1099 t
ORIGIN      
        1 ttctggggag gtccaggagg ggaacagccc ctcctgcgta ttcgatcagt caacgacttt
       61 tccgccgaca gcggaaaagt ccaggagggg gctccgcccc ctcttgcatc tcatcgggcg
      121 aaccgccttt ccgcgccatg cgcggaaagg cgagccttgt cctcggcact gaaggtaatc
      181 cgctgtggct gcttcgttcc cgacctgacc agattcacgg cacctccatg cgagggggcc
      241 cgccgggacg aatggtatca gtgcatgcgc cgacgccgag agctctattt cactgcaaag
      301 cagtaaaggc gtccgtcccc gccggtgctg ttgagctttg ccatatcgca gccgagtgtc
      361 gggtgcgacg aattccagga ggtcgtccag gtcgaggtca tcatcgggcc agcacgatcg
      421 tgatggccga ccatcgccga cccgtcggca ccgcccttgg tccagttacc gcaggtcatg
      481 tcaggaaatg gcgtaccggg aaacgcggtg ccatcgggtc gtgagcccgt cagtatgtcg
      541 tgccggtttg gcttgtccac cgtcccattg acgacctggc cgcgttcgtc gagtgctgtc
      601 tgcttggcca ggttgctcgc cggcgaatgc aggtcgtcga cactcgttgc gaccgtcacg
      661 cccatgacgt tctgccaggg acccgtgccg atacggtcgc gcgcattgac gaagttcgga
      721 tcccgcaacc ccggagcctg cgtgctcagg taggcgcgcc aggtgcgatc gagcgcaccc
      781 gcggccttgg cgagcttcgc gcaatgctga tcggcgccct cgagtccgcc gagatcagcg
      841 cccttgccgg gaccgacgct ggtcacgaag aaggtcatgt ccttcgggtt caactgaggc
      901 ggggcgctct ggcaacccca gagcgccgtc agacctaccg tcgccagccc cgcaattcgc
      961 agtgtcgagt gcattacgat ttcctgtcga ctctagtatg gctctccgcg cgctgcagcc
     1021 gcgagtcctg cggccggcgc agccaccgac ggccattctt ccggcagggc gcgtccaagt
     1081 caacgctcgt cgaccctgat agacactcca gaaggcacct caatcagttc gccagccgcg
     1141 gtgaccaggc ccatgcgcac gggcctgtcg ccccgtatgg aacgcaacac atcggcatag
     1201 cgacgcacct gtgcttcgta cgccggtcgc tcagcatcgg tcacctgcca cttgaagtcg
     1261 atgatccaca acgcatcgtc gcgctcgacc agtcgatcca cacgcagcag ttcgccgtcg
     1321 tcggcgacca gttcgagttc cgccacgtcg cgggcatcaa agaggcccca atgcacggcg
     1381 cggacctgcg ctgccgcggc aaccacagtg tcacgctgct cctgcgtcaa cgcatgcgca
     1441 cgcgcaatcg catcgaccgg tgcgtcgcta ccgtgctcga ggagcgcatg ccatgcgcgt
     1501 ccgagtcgca tggcgtcgga ctcaccggcc gcctccctcc ggcctcctgt cggcaacgga
     1561 tcgggaagga agtcgcgcag gattcgaacc ggcaacggag gtgcaagcgc aacggcggcg
     1621 gccgcgcccg atgacaggtc ttcagcacac tgcagcctcg tgtaccaggt gtcgtcgagc
     1681 gaaccgcggg acggcgcact gccggagacg atcaggacct gccgcgcacg cgtagcggcg
     1741 acgtacagca gattccagtc ctcctgtcgg cgttgttctt cgtcgcgctc gaaccagtcg
     1801 cgcctcgcaa ggtcgcgcgc ttcctcgccg cgggtgacca gggagaagtg ttcgggcgcc
     1861 ggctcctggg gcggccagtc gacaagcacc ccttcgcctt ccaacggcgc gcgtacatgg
     1921 gcgtcggcca gcgcaacgat ttcggcttcg agccccttgg caccgtggat cgtcatcacg
     1981 cgcacggcat cttcggcgcc ggtcgggccc tcgtccggcg cttcgtcggt cgcgtggtgc
     2041 cgcagccctg ccagttcatc gatgaagcgt ggcaggctgg gataacggcc ggcatcaatc
     2101 gcgagggcca gttcgataaa cgcgtcgaga ttggcctgca cctgtgcgtg aagggcgtcc
     2161 ggcacggccg cggcatatcg tctgcgcaga tccccctcga agtagatgcg atccagcaga
     2221 tcgtgcactg gcaggacccc ggccaggtcg agccaccaga gaaacagttc gcgcgcgcgc
     2281 gccagcgggg cggacaccgg atccgggagt tgctgcaggc ggtcccacca gcttgcgccg
     2341 ctcgcggcag cgagtcgcat caggtcgtcg tcactgcagc cgccgatggg cgagcgcagc
     2401 gcatgcgcga gctgaagatc cgaatacggg gcggtgagga agctgagcag cgcggtcagg
     2461 tcggcggctt cgagtgtggc gagcagtccg ccacgacggt cgctgatgaa tggaatgccg
     2521 gcatcgcgca gtgcccgttc gtagtcggca agatgggtcc gtcgacgcac aagcacgagg
     2581 acatccgacc agcgcgccgg acgaagaacg tcatcgtcca ccacctgcag gcgcagcttc
     2641 catgcggcaa tctcgttggc gagcacgcgc ccctcgcgat agcgcgtgtc cgtttcgcgc
     2701 tccgcccgcg gcgtagtcag cacatcgcgc agcgagatcc cgtctccggc catctcggtg
     2761 gccggctcgg gcgcttccag aggcagcagc acgaaggcac cttgcccgat ggagtaggga
     2821 gcgcgagtgc tctgcggctg gtagagcggg ttgccgcgcg gcatggcccg attgagaacc
     2881 tcgatcaccg ccggtgcatt gcgccgcgtg acgtttgtgc gcaagtggca cgcggcgtag
     2941 tcccggcaca gcagttcgag ggcggcgtcg aagacgcgtg gctcggcccg acggaagcga
     3001 tagatcgact gcttcgggtc gccgacgacg aacaccgtcg gtcgatcctg cgaagagccc
     3061 gagccagcgc ccacgtcctg cacaccgtag gccgccagcc atgactgcag aacctgccac
     3121 tgcagggagt tggtgtcctg aaactcatcg agcaggacgt gccggtagcg agcgtcgagc
     3181 cgggcgtgca tgtaggccgc gttgtcgggg tcggacagca agcggtaggt gtgccattcg
     3241 aggtcggtga agtccagtgc ctggcgctgc tgcttcaatg cctggtaggt atcgaccagc
     3301 aactgtccgc aggtcagcgc ggcatggttg actttgaagg cgcgccaggt tcggcgggca
     3361 tccatgattt gctgcaggcg gacgacgaca gccgcatgtg cattggcgta actgcgcgcc
     3421 tgcgtgtcgt tcgcgaaccg ggctgccatg cattccggcg tcagcaacac gatcggcgtg
     3481 gaccggccct tcgtgagcac gacgaggcag gccacgcgca agtcgcgggc gggattcttc
     3541 cccggcgacg ccagccatgc gctggcgcgc ccgaccgcca tgtcgatggt ccccagcgga
     3601 ggccggatcg attgccactg cgccaccagg ccgcgcaagc tcccggtgaa gtccggtgcg
     3661 cgcagttcca tggccggatc ccggccaccc gacaccctga gcggctcaag cgcccggtcg
     3721 acggctgccg gctgatcgcc ggccgcaaag ctccaccact ccgcgcgctt gtggagaaac
     3781 tgctgcagta gccgcctggc cgtgacttca ccggtttcgc cgatcaggtc ttcccacgcc
     3841 gcgcgctccg ccgcgcgctc cggccggatc agcgccgcgg tgaagtgcag ccaggcatct
     3901 gcccgcacgc gctccgagcc ttcgagcagg accggcgcaa acggcacccc cgcgcccagc
     3961 ggtgcacgcg caatgagctg ccagaaccat ccgtgaaagg tctcgatggt caatggcacc
     4021 cgcgcggtga cgacgcgctc gtaaagcctg cgcgcatcgt cgatggcggc aaccgcctgc
     4081 gcgcgtgaca gtccgcgctg acgcaggctc gcaacgatct tgtcgtcggt ggcgctggcg
     4141 agatcccgga ggtcgcgcag cagtcgcacc ttcatttcct gcgcagcgcg gcgtgtgaag
     4201 gtaatggcca ggatctcgcc cggcgcggtg cccgcgagca acaggcgcac gatgcggctg
     4261 acgagcagcc aggtcttgcc gctgcccgcg catgcctcga cgacaacgct cgaacgcggg
     4321 tcgcacgccc ggcgcgtgaa gtcgtcctcg tcgattggaa tctcgtccgc aaggtagtcg
     4381 ctcatgcgaa cggatccccg ctgtccgggt cgccgctctc cccgccatgc tccccgctgt
     4441 gcttccaata gtcgcgacgg cacaggccgc gcatctcgca gttcacacag acggactcgg
     4501 caccgatcgc cggcatcgcc acgccatcga ccatgcgttg aaggtcggcg gtcagcctcg
     4561 cttcgacgac gtcgatcagc tcgtcgaacg caccggtcgg cgcgaccgac ttcacacccg
     4621 aggtgtcttc ccggacacga tcgaagctga catattcacc ggacccgggc cgccgcggta
     4681 acagcattcc gtagaacggc aactggatgt cctctcccgg cgacttcagt ccacgcttga
     4741 gcacgtcggc ggcgcgcgcc ttgtagtcca gcagccgtag tcggccgtcc gcccgcgtgt
     4801 cgacgcggtc gacccggccg atcagttcga ctgcgtgccc cgcgttcagg acgatcggcc
     4861 gacgatggtt ctcttcaccg ccggtccaga gccagccgtt gccggcgtgc tgctgcagcc
     4921 agtcgatgta gccatcgacc aggccgtcaa agcgccgctc gaaggcaagg agccccggag
     4981 tgcgctcgat ctgcggaccg aagaccgcgc gcgcgtgctc gcgcaggctt gcgaccagtt
     5041 ctactggcgc gacaccgctg aagtcgacgg cgccccatgt tttgtggaaa cggctcagga
     5101 cctcatgcaa ggcgatgccg aactcccgtt tctcgggaag ttcaatcacg tcgtcgggcg
     5161 cccttagccc gagcatgcgt cgcgcataga actgatacgg acagtccacg aggctctgcg
     5221 cctggctggc actgatgcgc gcgggcagca gctgcgacgc gcggggcgcg ggacgtgtcg
     5281 tgaccgcgac ctcgacatcg acgtattccg gcagcgccgg tcgcgtcagg tcgtcgccga
     5341 gcacccgctg tgcgatgaag cgcagcctgt ccagcagagg cgacagcgga ctgggatcgt
     5401 caccgcggca tgtgcgccat gtcgctacga cctgggtcgc ggtcgccagc agcgtggcga
     5461 gttgcgcggc ctggtcgagc accgcgcttt cggcagtcgt cagcccgagt tctgcccgta
     5521 ctgcattcga catgaaaaga acctcggatg gcctcgaggg cagatgctgt gcatcggcac
     5581 cgaccagcac cgcggcgtcg aagctgcgca gtgcagcgga cgcgagcgac accatgacca
     5641 cgggtgattc gatccgtcga tcgacgaacg ccacctcttc gaagcgcgat gccagcagcg
     5701 cacgaaagtc ggcgagcgat gcgcgcccac cgagggtggc aagctccgct tccagcgctg
     5761 caaggtcgat cagcaccgcc tgcccgaccg gatcggcagt cagcgcggca cgcatgccga
     5821 gcgcatccat cgcctggcgc agcgcctgaa catgggaatg caacgtcggc gctgctcggc
     5881 gagtggcctg ggcctgggac tcgaccagcg cgatcacttc cagagccccg acgccatgtt
     5941 cgcgcgcttc gccggatcca tgattcgcca gcgcacgccg gatggacttt gcgccctgca
     6001 gtgctccggc ggcccggatg gcccgctcga tgcggtcgac ctcttgcgac ttgtcgggcc
     6061 gccccgacaa cgtgaagttc gacttcagcc agtccagcag gtcacgccag tacaggtcat
     6121 cggccacgac gtcgtaccac cgcatgatcg cggcggcggc actggtggtg gagagtttcc
     6181 agcccgtctc atcgcgcacg ctgacctgcg cacgttccag cagcgcgcgc acccggcgtg
     6241 ccgtcagacg atccagcgcg acaagggcga tcgaccccac acccgcgcgc cgccactcga
     6301 caacctgctg cgctacagcg attgcctctt cttccagcgt gagcgccttc acgatcgaca
     6361 aaggtgtcga cggccggggc gcggcgggga tggcatcggc gcggacggcg atggccacgt
     6421 cggtgtcgac gcccccgagt tccggccagg cggccgccag cagcggttga tcctctaggg
     6481 cagcagtcac gtcggggtca agcagcagca ccggcgcctg ctcggccgcg cgatcgatga
     6541 atgcgcgctc ccacgtcgcc agtcccgccc gcccggcggt ggacagccct gtgccgagat
     6601 acaccagcgg cagctgggtt cgcgatgcgc gcgctgcgag ttcccgcagg aaccgcgcgg
     6661 cgccgtcgtc ggcctcgcga cgggcgcgcc agagctggat gaccagttgc gactgcgcct
     6721 gcagcgcatg cgccgcacgg cgatggaagt gccgccgcag gctagtctgc agcctgccgt
     6781 cgaaatcctc ggcaccgctg actgaagcga gcgtcagttc atcgcacaag tgagccacat
     6841 cacgcgcgag tgcccacaag gtc
//
LOCUS       DATIRT010000355         3840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1923525, whole genome shotgun sequence.
ACCESSION   DATIRT010000355 DATIRT010000000
VERSION     DATIRT010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1923525"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2408)
                     /locus_tag="VLN25_08050"
     CDS             complement(<1..2408)
                     /locus_tag="VLN25_08050"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HSQ10552.1"
                     /translation="MSKIVCRPRALTPDQAAKVLRRSIEINPANADEQRILARTPVGR
                     RGGPRRLAVVIGHKWPETGVRLTVQFLDNPSRDLRARILLHMNAWSKTTNVQFVETAS
                     VGQVRIARLDEPDDMAGYWSYVGTEILGIEEDQPTFNLDGFTMRTPEAEFRRVVRHEA
                     GHTLGFEHEHMRAELIKKIDRRKAFAYFDRTEGWTAQETLEQVLTPLAKKSIMGTAES
                     DPHSVMCYQIPAEITKDGKTIPGGKDITAKDYAFAATIYPLARPARDTTAAVTDSAAS
                     TPAVWTPSAPVTLPTVLPAVYGDTFHIVVMDRFEPDVAGSAAAARRQKYARVFATYGG
                     ARVTIPMRLNTDKGEERTRFRAIIDMHERIKAYTNRDSGTLPDDAELINFGTDLFETL
                     FQGDVRRLYDEARSRQHGRKLDFVFTSMVSWIAEKPWEFAYDASRRSFLATEDIHFVR
                     NVLTAIPADVIPPRAGPLRILVASAQPAGFGRLSAEQELEVIRRGFDPLIDAGLAEVE
                     VLPRATPSSIHARLSTGSFTIVHFIGHGVFDDKTQEGSLVFEDTRGGEYLLGERSVRE
                     IFCQRGVTLVFLNACQTGSGGRADFNKGVAQSLVAHGLPALVANQYSVLDSSATSFAQ
                     HFYWALAQGMTIGQAAREARIAVNYSLQGEMIDWAVPVVYARDPNMTLSVRPHGLTGL
                     ASPRMRAKSRRAIAPRPVEVAVWDIDNVFPSLKDTLERLTGAQEQFGFELVDLSAPID
                     AWDLEERADDGTPYLWAEKLANRLGRVPLELHVNVLACVTRHWLRDDDTLNIYGWWPD
                     KGEGP"
     gene            2637..3746
                     /locus_tag="VLN25_08055"
     CDS             2637..3746
                     /locus_tag="VLN25_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10553.1"
                     /translation="MSNDAEPRFSGMPAAPAPEVFADARRNNPEWFQLVERAYEQFAA
                     LPENVGVYADPKGSGATIEQIWLVLTLFGVEPYCLLARPLPAVSTKRISRLVNCKATQ
                     IIEALKTIQAVNGEVNVPLAYHDGKTGHCIRITDYDSGRDRFIYHDPWPERSLLAKEN
                     NPAGVDAQPDGTRWSVTAQELERVVFAAFAFPSQWARVRGQDFDILVDPWKDSDFFKF
                     FRLKQLDERAVGEHTQRSFAPGAFQQDIALKVECNKVGKIKRASLLLTQDWTIGNFML
                     ALDLAKSFVASFAPAPDRTRYDEIAAALWSLRDPRALLKAKETDPKESDGVRCVHAFM
                     GSLESASIVTDFSHLSIGSTTHDGRPARLIAIDLS"
BASE COUNT          677 a         1265 c         1223 g          675 t
ORIGIN      
        1 ggtccctcgc ccttatccgg ccaccagccg tagatattga gggtgtcgtc atcgcgcagc
       61 cagtgccggg tgacgcaggc gagcacgttc acgtgcaatt ccagcggcac gcggcccagc
      121 cggttcgcaa gcttttcggc ccacagatag ggagtgccgt cgtcggcccg ttcctcaagg
      181 tcccatgcat cgatcggggc cgagagatcc accagctcga aaccgaactg ctcctgcgcg
      241 cccgtcaagc gctcgagcgt gtccttcagc gacgggaaaa cgttgtcgat atcccagacc
      301 gccacctcga ccggccgcgg cgcaatggcg cgacgggact tcgctcgcat cctgggcgac
      361 gcgagtccgg tgagcccatg cggccggacc gacagcgtca tgttgggatc gcgggcgtac
      421 acgaccggaa cggcccagtc gatcatttcg ccctgcagcg agtagttgac ggcaatgcgc
      481 gcttcgcggg cggcctgccc gatcgtcatg ccctgcgcca acgcccagta gaaatgctgc
      541 gcgaagctgg tagccgacga gtccagcacg ctgtactggt tagcgaccag cgccggcagg
      601 ccatgcgcca cgagcgattg cgccacgccc ttgttgaagt cggcgcgtcc gccggagccg
      661 gtctggcagg cgttcaggaa taccagcgtg acgccgcgct ggcagaagat ctcgcgcact
      721 gatcgctcgc cgagcaggta ttcgccgcca cgtgtgtctt cgaacacgag cgagccctcc
      781 tgcgtcttgt cgtcgaacac tccatggccg atgaagtgca cgatggtgaa gctgcccgta
      841 gacagccgtg catggatgct gctcggcgtg gcgcgcggca gcacctcgac ctcggccagc
      901 cccgcgtcga tcagcggatc gaagccgcgc cggatgactt cgagttcctg ctcggccgag
      961 agacggccaa agcccgccgg ctgagccgac gccaccagga tccgcagcgg ccccgcgcgc
     1021 ggcgggatca catcggccgg tatggccgtc aggacattgc ggacgaagtg gatgtcctcc
     1081 gtggcgagaa agctgcggcg agaagcgtcg taggcgaact cccacggctt ctcggcgatc
     1141 cacgacacca tcgatgtgaa aacgaaatcg agcttgcggc cgtgctgccg cgagcgcgcc
     1201 tcgtcgtaca gccgccggac atcgccctgg aacagcgtct cgaacagatc cgtgccgaaa
     1261 ttgatcagct ccgcatcgtc gggcagtgtg ccggagtcac ggttcgtgta cgccttgatg
     1321 cgctcgtgca tgtcgatgat cgcgcggaac cgcgtgcgct cttcgccctt gtcggtattc
     1381 agccgcatgg ggatcgtcac gcgggcacca ccgtaggtcg cgaagacgcg tgcgtatttc
     1441 tgccgacggg ccgcggcggc cgatcccgcc acgtcgggct cgaaccgatc catgacgaca
     1501 atgtggaaag tgtcgccgta cacggccggc aggacggtgg gcagtgtcac gggggccgag
     1561 ggcgtccata ccgcaggggt cgatgctgca ctgtcagtca cggccgccgt cgtgtcccgc
     1621 gcaggtctcg cgagagggta gatggtcgct gcgaacgcgt agtccttggc ggtgatgtcc
     1681 ttgccgcccg gaatggtctt gccatccttc gtaatctcgg ccgggatctg gtagcacatg
     1741 accgagtgcg gatcactctc cgccgtgccc atgatcgact tcttcgcgag cggcgtcagg
     1801 acctgctcga gcgtctcctg tgcggtccag ccctcggtgc ggtcgaagta ggcgaaggcc
     1861 ttgcgccgat cgatcttctt gatgagttcg gcgcgcatgt gctcatgctc gaatcccagc
     1921 gtgtgaccgg cctcgtgccg caccacgcga cgaaactccg cctccggcgt gcgcatcgtg
     1981 aagccatcga ggttgaaggt cggctggtct tcctcgatcc cgaggatctc cgtgccgaca
     2041 tacgaccagt agcccgccat gtcatcgggc tcatcgagcc gggcaatgcg tacctgcccg
     2101 acgctcgccg tctcgacgaa ctgcacattc gtggtcttcg accaggcatt catgtgcagc
     2161 aggatgcggg cacgcaggtc gcgcgatggg ttgtcgagga actgcacggt gagacggacg
     2221 ccagtctcgg gccacttgtg tccgatcacg actgcgaggc gcctgggtcc gccgcgtcgt
     2281 ccgacgggcg tcctcgcgag gatgcgttgc tcgtccgcat tggccggatt gatctcgatg
     2341 ctgcgacgta gaacctttgc cgcctggtcg ggcgtgagtg ccctcggcct gcagacgatc
     2401 ttggacatga gcgatgggac tccgttgtga ctccgccgtg actcgatagc gtaggcgaca
     2461 ccaaatcatg ggcccaattc gatcaggctg ccaaggtccg caccttgatc gatggcagat
     2521 ccattgccaa ccggcccgga cgacggcgct gccacaatcg cccctgaccg aggcgcgcgg
     2581 tgcgcgatca cacgtgccct tcgacgtccg gaacctcact gagcaggggg aacatcatgt
     2641 ccaacgatgc ggagccgcgt ttcagcggca tgccggccgc cccggcgccc gaggtgttcg
     2701 ctgatgctcg ccgcaacaat ccggagtggt tccagctcgt cgaacgtgca tatgaacagt
     2761 tcgccgcact cccggagaac gtcggcgtct acgccgaccc caaaggaagt ggcgcaacga
     2821 tcgagcagat ctggctcgtc ctgacgctct tcggcgtcga accgtactgc cttcttgccc
     2881 gccctctgcc cgccgtctcg accaagcgca tttcacggct ggtgaactgc aaggcaacgc
     2941 aaatcatcga agcgctcaag acgatccagg cggtcaacgg ggaagtcaac gtccctctcg
     3001 cctaccacga cggcaagaca ggccattgca ttcgcatcac ggactacgac agcgggcgcg
     3061 atcgtttcat ctatcacgac ccctggcctg agcgctcgct gctcgcgaag gagaacaacc
     3121 cggccggggt cgacgcccag cccgacggga cgcgctggag cgtgaccgcg caggaactcg
     3181 aacgcgtcgt gtttgccgcc ttcgcgtttc ccagccagtg ggcgcgggtg cggggtcagg
     3241 acttcgacat cctggtcgac ccgtggaagg acagcgactt cttcaagttc tttcgcctga
     3301 agcaactcga tgagcgcgcc gtcggcgagc atacgcaacg gtccttcgca cccggagcgt
     3361 ttcagcagga catcgcgctg aaagtcgagt gcaacaaggt cggaaaaatc aagcgagcct
     3421 cgctgctgct gacgcaggac tggacgatcg gaaacttcat gctggcgctc gatcttgcaa
     3481 agagcttcgt ggcgagcttt gcgccggctc ccgaccggac ccggtatgac gagatcgccg
     3541 ccgcgctatg gagcctgcgt gatccacgcg ccctgctcaa ggcgaaggag acggacccga
     3601 aggaatccga cggcgtacgc tgtgtgcatg ccttcatggg ctcgctggaa agcgcgagca
     3661 tcgtcaccga tttcagccat ctttccatcg gcagcacaac ccacgacggg cggccggcac
     3721 gcctgattgc catcgacctg tcgtgagtgg ctgaattttt gagtgccgcg ggcgggcgcg
     3781 ccgctcgcgg cactgcccac ccgtgctggc ctcagtcgaa actgactgtc aatccgtcgg
//
LOCUS       DATIRT010000356         5077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_252791, whole genome shotgun sequence.
ACCESSION   DATIRT010000356 DATIRT010000000
VERSION     DATIRT010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252791"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VLN25_08060"
     CDS             <1..221
                     /locus_tag="VLN25_08060"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HSQ10554.1"
                     /translation="PPRWNVGGAPIGIGGLAVTAAFVALHGVRPGLLLGVAPAAIIYL
                     WLRARTGSLLAPIAAHLLWNLSLVLMYR"
     gene            285..1415
                     /locus_tag="VLN25_08065"
     CDS             285..1415
                     /locus_tag="VLN25_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010093147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HSQ10555.1"
                     /translation="MSTLFSPYTLRSLELPNRIVISPMCQYSALDGSATDWHWAHLGS
                     LAMSGAGLLCIEATAVTSAGRITQGDLGLYSDANEDALAGVLRMIRAVAPIRVAVQLA
                     HAGRKASSREPWNGGRLLPRDQGGWLPEAPSALAHGPDESPPRALDEDDLARLRTAFA
                     EAATRAARLGIDAIEVHAAHGYLLHEFLSPVANQRTDRYGGSLENRMRFPLEVFEAVR
                     AAFPSDRPVGMRISATDWLEELEPAPPSWTLEQSLALARELKVRGCDWIDVSSGGVSP
                     LQRIKPAPGYQVPFAEIIRNEIGMATMAVGLITEPRQAEAIVAEGRADLVALARSMLF
                     DPRWPWRAAIELGGVVAGPRQYGRSLPREAATIFGPLTFGQR"
     gene            1504..2652
                     /gene="ribBA"
                     /locus_tag="VLN25_08070"
     CDS             1504..2652
                     /gene="ribBA"
                     /locus_tag="VLN25_08070"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018057846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HSQ10556.1"
                     /translation="MNALSLVTGRSESSGSIAAVPEILDELRNGNIVVLVDDEDRENE
                     GDLVFAADFVTPEKINFMAKHARGLVCLTLTDAHARQLALPPMARANGTVHGTNFTVS
                     IEAAEGVTTGISAADRARTVWVASRTNAKPADIVQPGHIFPVVAQAGGVLMRAGHTEA
                     GCDLTQLAGLTPAAVICEIMNDDGTMARLPDLIPFAQTHGLKVGTIADLIHYRAATES
                     IIERCGRRRVQLPAGEFELVVYRDKPSGAPHLALVKGVIDPERESLVRVHEPVSVLDF
                     LDATASTHSWPIPKALAAIAQAEAGVLVLLNCAEPSDRLFAKFDALVSSESGDDGARQ
                     ARMDLRTYGIGAQILRDLNVGQMKLLARPRKMPSMAGFGLHTTEFIES"
     gene            2717..3202
                     /gene="ribH"
                     /locus_tag="VLN25_08075"
     CDS             2717..3202
                     /gene="ribH"
                     /locus_tag="VLN25_08075"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HSQ10557.1"
                     /translation="MAIDYLAPDFDGADLRIGIVQARFNEWAGRALMEACVSELVTLG
                     VDEDDVTHLTVPGSLEIPVALAKLASTEDFDALIALGCVIRGETFHFEVVSNESARGV
                     AQVALTYGVPVANGIITAESDEQARARVGEKGRDAARVAVEMANLLWALDENGDETVV
                     D"
     gene            3177..3680
                     /gene="nusB"
                     /locus_tag="VLN25_08080"
     CDS             3177..3680
                     /gene="nusB"
                     /locus_tag="VLN25_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HSQ10558.1"
                     /translation="MATRPSSTEPPTAAPRGGSRSARRRARELAVQGLYEWLVGGQDA
                     AAIEGHLAETRGADGLEELTARTDMQHYRELLNGAIGGAGALRADFAPHLDRGVEALS
                     PVEHGILLLATYELKHRAEIPYRVVINEAVELAKSFGGTEGFKYVNGVLDKVAAQLRP
                     AEVAARA"
     gene            3703..4692
                     /gene="thiL"
                     /locus_tag="VLN25_08085"
     CDS             3703..4692
                     /gene="thiL"
                     /locus_tag="VLN25_08085"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HSQ10559.1"
                     /translation="MASTRQPAGEFELIARYFERPGAVASRVDLGIGDDCALIDGGPQ
                     MQWAVTTDMLVEGVHFLAAVDPEALGHKALAVNLSDLAACGATPRCFFLALAMPRPDE
                     AWLAAFTRGMFALADAHACALAGGDTTRSPAGVTLSITALGDLPRGSALLRSGAVPDD
                     DLWVSGVLGDGALGLAYQRGDIVLPAGTARAFVERLERPTPRVALGQRLRGIATSAID
                     VSDGLAGDLGHILDRSGAAATVEWTSIPRSDGLRELDALVQQHCVFAGGDDYELLFTA
                     PRSASDAVRAAGLAAGVAVSRIGAIRAGSGLTVVDADGRAMDREARAFDHFRP"
     gene            4689..>5077
                     /locus_tag="VLN25_08090"
     CDS             4689..>5077
                     /locus_tag="VLN25_08090"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HSQ10560.1"
                     /translation="MNAPTSPMTAAPVHPDEAATGNTAPSMRFMLAHPAHGVALGFGS
                     GLLRPAPGTWGTLAGWGAFIALEGWLSPVAWGVVIAFTFLIGCASAQRTGAALGRPDH
                     GAIVIDEIVAIWIVLLLLPSTLIAQACG"
BASE COUNT          757 a         1708 c         1763 g          849 t
ORIGIN      
        1 atccgccacg ctggaatgtc gggggcgcac cgatcggcat cggcggcctc gccgtgaccg
       61 cagcgttcgt cgcgctgcat ggcgttcggc ccggcctgct gctgggcgtg gcgccggcgg
      121 cgatcatata tctttggctg cgtgcgcgca cgggcagcct gctggcacct atcgccgcgc
      181 atctgctatg gaacctgtcg ctagtgctca tgtatcgatg acgattgatc gagctccaga
      241 ccaacccgtt tttcccgact catcctccaa gcccgattcc ttccatgtcc acgctcttca
      301 gtccctacac cctgcgctcg ctcgaactcc cgaatcgcat cgtcatctcg ccgatgtgcc
      361 agtactccgc cctcgacggc agtgccaccg attggcactg ggcccacctg ggatcgctcg
      421 cgatgtccgg agccgggttg ctgtgcatcg aggcgacagc ggtgacgtcg gccggtcgca
      481 tcacgcaagg ggacctcggt ctgtacagcg atgcgaatga agacgcgctg gccggcgtgc
      541 tgcggatgat ccgagcagtg gcaccgatcc gggttgcggt ccagctggcc catgccgggc
      601 gcaaggcgtc gagccgtgaa ccgtggaacg gcggcagatt gttgccgcgc gatcagggag
      661 gttggctgcc ggaggcgccg tcggccctgg cgcacggacc cgacgaatcg ccgccgcgcg
      721 ctctcgacga agacgacctt gcgagacttc gcaccgcatt cgctgaagcg gccacgcgcg
      781 cggcgcgact gggcatcgat gcgatcgaag tccatgccgc gcacggctac ctgttgcacg
      841 aattcctgtc gccggtggcg aaccaacgca ccgatcgcta cggcggatcg ctcgagaacc
      901 gtatgcgttt cccgctggag gtgttcgagg ccgtgcgcgc cgctttccct tccgatcggc
      961 cggtcggcat gcgcatttcc gcaaccgact ggctggagga actcgagcca gcgccgccga
     1021 gctggacgct cgagcagtcg ctggccctgg cgcgcgagct caaggtacgc ggatgcgact
     1081 ggatcgacgt atcgagcgga ggcgtgtcgc cgcttcagcg catcaagccg gcgccgggct
     1141 accaggtgcc gttcgccgaa atcattcgca acgagatcgg aatggccacg atggccgtcg
     1201 gcctgatcac cgagccgcgc caggccgagg ccatcgtcgc cgagggccgc gccgacctgg
     1261 tcgcgctggc gcgcagcatg ctcttcgatc cgcgctggcc atggcgggcc gcgatcgaac
     1321 tcggcggtgt ggtcgctggc ccacggcagt atgggcgatc gctgccgcgc gaggctgcca
     1381 ccatcttcgg cccgttgacg ttcggccagc gctgacggtg ggcgccggcg cgggtgggct
     1441 gcacgaggct gggctgcacg aactagaatc gctgccttgc ggggggcgtc cccgggaggt
     1501 cgcatgaacg cactgtcgct ggtcacgggt cgatcggagt cgtccggttc gatcgcggcc
     1561 gtgcccgaga tcctcgacga actgcgcaac ggcaatatcg tcgtgctggt cgacgacgag
     1621 gatcgcgaga acgaaggcga tctcgtcttc gcggcggact tcgtcacgcc ggaaaagatc
     1681 aacttcatgg ccaagcatgc gcgcggactg gtctgcctga cgctgaccga tgctcacgcg
     1741 cggcagcttg cgctgccgcc gatggcgcgt gccaacggca ccgtgcatgg cacgaacttc
     1801 acggtctcga tcgaggctgc ggagggcgtg accacgggaa tctcggcggc cgatcgcgca
     1861 cgcacggtct gggttgcgag ccggacgaat gcgaagccgg ctgatatcgt gcaaccggga
     1921 cacatctttc ccgtggtcgc gcaggccggc ggtgtgctga tgcgcgccgg acataccgaa
     1981 gccggatgtg acctgacgca gctggctgga ctgacgcccg cggccgtgat ctgcgaaatc
     2041 atgaacgatg acggcacgat ggcgcgcctg cccgacctga tcccgttcgc ccagacgcat
     2101 ggcctgaagg tcggcaccat cgccgacctc atccactacc gcgcggcgac cgagtcgatc
     2161 atcgaacggt gcggcaggcg gcgcgtgcag ttgccggcag gagagttcga gctcgtcgtc
     2221 taccgcgaca agccgagcgg cgcgccgcac ctcgcgctcg tcaagggtgt catcgacccg
     2281 gagcgtgaat cgctggtgcg tgtgcacgaa ccggtctcgg tgctcgactt cctcgatgcc
     2341 actgctagca cacactcctg gccgatcccg aaggcgctgg cggcgattgc gcaggccgag
     2401 gccggtgtgc tggtgctgct caactgcgcc gagccgtccg accggctctt cgccaagttc
     2461 gatgccctgg tgtcatcgga gtccggcgac gacggtgcac gccaggcccg catggacctg
     2521 cgcacttacg gcattggtgc gcagatcctg cgcgatctca acgtcggcca gatgaagctg
     2581 ctggcgcgtc cgcgcaagat gccgtcgatg gccggcttcg ggctgcacac gaccgagttc
     2641 atcgagagct gaactcgcgc gtcgccgcag gcgtcacgcg aaaccgctcg cgtacactgc
     2701 gtcgcctgga ggacacatgg cgatcgacta tctcgcgccc gacttcgacg gcgctgatct
     2761 gaggatcggc attgtccagg cgcgcttcaa cgagtgggcc gggcgggcgc tgatggaggc
     2821 ctgcgttagt gagctggtca cactcggcgt cgacgaagat gacgtcacgc acctgaccgt
     2881 gcccggatca ctggaaatcc cggtggccct ggccaagctc gcctccaccg aggacttcga
     2941 tgcgctgatc gcgctcggct gcgtgattcg tggcgagact ttccacttcg aagtcgtgtc
     3001 caacgagtcg gcgcgcggag ttgcccaggt cgcgttgacc tacggcgttc cggtcgccaa
     3061 cggaatcatc actgccgaaa gtgacgagca ggcgcgcgcg cgcgtcggcg agaaaggccg
     3121 tgacgcagcg cgcgttgccg tcgagatggc gaatctgctc tgggcactgg atgaaaatgg
     3181 cgacgagacc gtcgtcgact gaaccgccga ccgcagcccc tcgcggcggc agccgcagcg
     3241 cccgacgtcg tgcgcgtgaa ctggcggtgc agggtctgta cgaatggctg gtcggcggtc
     3301 aggacgccgc tgccatcgaa gggcatctgg cagagacacg cggcgccgac ggtctcgaag
     3361 agctgaccgc ccggaccgac atgcagcact atcgtgaatt gctcaatgga gctatcggtg
     3421 gtgccggtgc gctgcgggcc gacttcgcgc cgcatctcga tcgcggtgtc gaggcactgt
     3481 cgccggtcga gcacggcatc ctgctgctgg ccacctacga gctgaagcat cgcgccgaga
     3541 ttccgtatcg cgtcgtcatc aacgaagcgg tggaactcgc caagagcttc ggcggcaccg
     3601 agggtttcaa gtacgtcaac ggcgtgctgg acaaggtggc cgcgcagctg cggccggccg
     3661 aagtcgccgc gcgcgcctga ccggcgaccg caggcccacg ccatggcgtc gacccggcag
     3721 ccggccggcg agttcgagct gatcgcacgt tacttcgagc gccccggggc cgtcgcatcg
     3781 cgagtcgatc tgggcatcgg tgacgactgt gccctgatcg acggcggtcc gcagatgcaa
     3841 tgggccgtga ccactgacat gctggtcgag ggcgtgcatt tccttgccgc tgtcgatccg
     3901 gaagcgctgg gccacaaggc gctggcggtc aatctgtcgg atctcgccgc ctgcggggcg
     3961 acgccgcgct gctttttcct tgcactcgcg atgccgcggc cggacgaagc ctggctggca
     4021 gcgttcacgc gcggcatgtt tgccctggcg gacgcgcatg cctgtgcgct cgccggcggc
     4081 gacacgaccc gctcgcccgc gggcgtgacc ctgtcgatca ccgcgctcgg tgacctgccg
     4141 cgcggcagcg cgttgctgcg ctcgggcgcg gtgccggacg atgacctctg ggtctccggc
     4201 gtcctcggcg acggggccct ggggctggcc taccagcgcg gcgacatcgt gcttcctgcc
     4261 gggaccgctc gtgcgttcgt cgagcgcctg gagcgcccga cgccgcgcgt ggcgctgggc
     4321 cagcgcctgc gcggcatcgc gaccagtgcg atcgacgtct cggatggcct ggcgggcgat
     4381 ctcggccaca tcctggatcg ctccggggcg gcggcaacgg tcgaatggac ctcgatcccg
     4441 cggtcggacg ggctgcggga actcgatgcc ctcgtgcagc agcactgcgt gttcgccggg
     4501 ggcgatgact acgaactcct gttcacggcg ccgcgctcgg cttcagacgc tgtccgcgcg
     4561 gcggggttgg ccgccggcgt cgcggtgtcc cgcatcgggg cgatccgggc tggcagcgga
     4621 ctgacggtcg tcgatgccga cggccgggcg atggacaggg aagcgcgggc gttcgatcat
     4681 ttccgtccat gaacgcgccc acgtcaccga tgacggccgc tcccgtccac cccgacgagg
     4741 cggctaccgg caataccgcg ccgtcgatgc gattcatgct cgcgcatccg gcgcacggcg
     4801 tcgcgctcgg attcggcagc ggcctgctgc gcccggcgcc cggcacctgg ggcacgctgg
     4861 cagggtgggg ggcattcatt gcactggagg ggtggctgtc tcccgtggcc tggggcgtcg
     4921 tgatcgcctt caccttcctc atcggctgcg ccagtgccca gcgcaccggt gccgcgctgg
     4981 ggcgcccgga ccatggcgcg atcgtcatcg acgagatcgt tgccatctgg atcgtgctgc
     5041 tgctgctgcc ttccacgctg atcgcgcagg cgtgcgg
//
LOCUS       DATIRT010000357         2996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1334756, whole genome shotgun sequence.
ACCESSION   DATIRT010000357 DATIRT010000000
VERSION     DATIRT010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334756"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..711)
                     /locus_tag="VLN25_08095"
     CDS             complement(109..711)
                     /locus_tag="VLN25_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10561.1"
                     /translation="MNRPACAVVASMSALVAAALLPNIADAQSRVQYGRITNVQATTV
                     TDNTSRAVGTLVGGGIGLATGSGQSGSNRALRGIGGAAAGRGVGSLAGRSQAFEYTVL
                     VGGTTTVRIVSDQAGKRIGDCVAVEQGQFSNIRLVPDGRCAPAASAAPAKKAAPAPAP
                     APADVQQATACEQAKDQVLAAATDEEFNVAERRMRLLCGE"
     gene            complement(856..1170)
                     /locus_tag="VLN25_08100"
     CDS             complement(856..1170)
                     /locus_tag="VLN25_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10562.1"
                     /translation="MINWRAREIMASALMAAIGAMWLSSAWSASDWAPPYNIALKLAF
                     AFICFAVVANPFVLFERMSFKRMFESREPSALAFAFLAGLGMLCAIAALVLWLSKPLW
                     SR"
     gene            1887..2465
                     /locus_tag="VLN25_08105"
     CDS             1887..2465
                     /locus_tag="VLN25_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HSQ10563.1"
                     /translation="MDKQRRNVLIAGAGLAVLTVATGCTTTSGTSGDPAAQRQSIDAA
                     ADSALSRLYAQQPGTRELVASSRGMLVFPSFVSAGFIVGGGSGQGALRKGGKTTGYFR
                     MTEASAGLLAGAQSQAVFILFMTEEALKRFEASSGWTAGVDGSVAMLNVGANAQVTTQ
                     TASQEIIGFVMTNSGLMGNLSLNGNRVTRLSI"
     gene            2611..>2996
                     /locus_tag="VLN25_08110"
     CDS             2611..>2996
                     /locus_tag="VLN25_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HSQ10564.1"
                     /translation="MLALAIDDMLRTTTNAMVLLVATGWLLHIGKGIFVPIVLSVLLV
                     YVIDGLARLFARVPVVGPQLPGQLRYALSIVLIAVILVYVARMLIASLGNVVALAPQY
                     ETSLLNAIQAMAERLGMEDAPTWATLR"
BASE COUNT          532 a          991 c          978 g          495 t
ORIGIN      
        1 ccggacgccc agctggccgt gaagtagcgt tgcccgtcgc cagccgcacg ctggtgcgcg
       61 cgaagcgaaa cggggcgctc acgcgccccg tttcgttttc ccgagagcct attcgccgca
      121 gagcaggcgc atgcggcgct ctgccacgtt gaactcctcg tcggtggcgg cagccaggac
      181 ctggtccttg gcctgctcac acgccgtggc ctgctgaaca tcagcggggg ccggcgcggg
      241 tgcgggcgca gccttcttcg ccggagcagc cgaagcggcc ggggcgcatc ggccatctgg
      301 caccaggcga atgttcgaga actggccctg ctcgacggcc acgcagtcac cgatgcgctt
      361 gcccgcctgg tccgagacaa tgcggaccgt ggtcgtgccg ccgaccagca ccgtgtactc
      421 gaacgcctgc gatcgtccgg ccagcgaacc gacccctcgg cctgcggcag cgccaccgat
      481 gccgcgcaac gcgcggttgc taccggactg gccgcttccc gtcgccaggc caatgccacc
      541 gccgaccagc gtgcccaccg cacgagacgt attgtcggtg acggtcgtcg cctggacatt
      601 ggtgatacga ccgtactgca cgcgagactg cgcgtcggcg atgttcggca gcagcgcagc
      661 ggccacaagg gcggacatcg aagcaacaac ggcacaggca ggacgattca tcgcacagct
      721 cctagggtag gtggacggcg tcaaccgcgt ccgaagtttg tgtcgatgat accgggcgaa
      781 aatgtgcgcc gcggctggcg gcccgatggc ggcaggaaca cggagcgcat ccggctagtc
      841 aatgcggacg cgaggtcagc gactccacaa tggcttgctc agccacagta cgagcgccgc
      901 gatggcgcac agcatgccga gtccggccag aaaggcaaag gcgagcgcac tcggttcccg
      961 gctctcgaac atccgcttga acgacatgcg ttcgaacaga acgaacgggt tcgcgaccac
     1021 ggcgaagcag atgaacgcaa acgccagctt caaagcaatg ttgtagggcg gcgcccagtc
     1081 gctcgcggac cacgcggacg acagccacat ggcgccaatc gcggccatca aagccgacgc
     1141 catgatttcc cttgcccgcc aattgatcat gcgcacccgc ctcccgtcgg tcgcgccacc
     1201 gctcacatcc tgtgtcgggc aggtggccag cgcctcgcgg ctgtcgtttg agtccgacaa
     1261 tacgctcgcg ccacttgctg cacagtcaac cttttgttct acgaggtccg gctagatcag
     1321 ccgcggaccg tcctctccgg gcggcgccga gattagtgca gaacgggcgc gctgcgtgct
     1381 tttcggtatc cccggaacag gggctagcgg accactttgg tcaacgacgc cgtcatcggc
     1441 ggtggtgaat caacccttgc ggcgcggccg ggttcgaccg cgggccgcgc gcgctttcgc
     1501 agcggctgac ttcaacaaac tcgtcttcga caaacctgtc ttcaacaatc ccaacaggcg
     1561 ctcctcttcg acagcgacgg cacgctccgc gagttgcact cgagcgggca cggcccggtt
     1621 cgagggcgac cgcaaaggat tcgatcaatt gcgcggtatc ctcgagccgt tcaagcccaa
     1681 cttcgagatc ctgccgggca cggcaccgaa gaagccgaca taggctccga agcccttcga
     1741 agtgcgtgaa cctgtcaacc cgatggtgag cgagtagcgc gttactgaat gtggcgggca
     1801 gccttggatg ttgcccgcct aaacccatgg ctccctcaac cgcgtcgatc acagcggcgg
     1861 cgagccttat tcgagcagag gatggaatgg acaagcaaag aagaaatgta ctgatcgccg
     1921 gcgcaggttt ggccgtattg actgttgcca ccggctgcac gacgacgtcc ggaacgagtg
     1981 gagatcctgc tgcccaacgt cagtcgatcg acgctgcggc cgacagcgcg ctgtcgcggc
     2041 tctacgctca gcagccgggt acgcgcgagc tggttgcctc ctcgcgcggg atgctcgtgt
     2101 tcccgagctt tgtgtcggcg ggcttcatcg tcggcggtgg cagcgggcag ggcgcgctgc
     2161 gcaagggcgg caagaccacc ggctacttcc gcatgaccga agcgtcggca ggcctgctgg
     2221 cgggagccca gtcccaggcg gtgttcatcc tgttcatgac ggaagaagcg ctgaagcgct
     2281 ttgaagccag cagcggatgg accgccggcg tcgatggctc ggtcgcaatg cttaatgtcg
     2341 gcgcgaacgc gcaggtcacg acgcagactg cgagtcagga gatcatcggt ttcgtgatga
     2401 ccaactccgg actgatgggc aacctcagcc tgaacggcaa ccgcgtcacg cgactgtcga
     2461 tctagaccgc agcggccaca ggcggctgag ggttcgacgc tcctccgctg aaggggcgcg
     2521 tgcccgcggc gcacggtacg atccgccggc gccggtacgg tcatcgaccg cggacgtgcg
     2581 ccgagcgggg cccgccgttc gattcggcag atgctggcct tagccataga cgacatgctg
     2641 cgaacgacga cgaacgcgat ggtcctgctg gtggcgaccg gctggctgct gcacatcggc
     2701 aagggcattt tcgtgccgat cgtgctgagc gtgctcctgg tctacgtgat cgacggcctc
     2761 gcacgtctgt tcgcgcgtgt gccggtcgtc ggcccgcagt tacccggcca gctccgatac
     2821 gcgctctcga tcgtgctgat cgccgtgatc ctcgtctatg tcgcgcgcat gctgatcgcg
     2881 agtctgggca acgtcgtcgc gctggcgccg cagtacgaga cttcgctgct caatgcgatc
     2941 caggcgatgg ccgagcggct cggaatggaa gacgccccca cctgggcgac gctgcg
//
LOCUS       DATIRT010000358         2696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_450346, whole genome shotgun sequence.
ACCESSION   DATIRT010000358 DATIRT010000000
VERSION     DATIRT010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450346"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..1236
                     /locus_tag="VLN25_08115"
     CDS             70..1236
                     /locus_tag="VLN25_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HSQ10565.1"
                     /translation="MLRRVWLIFAQATTIGLALLFVVSTLRPEWLARTPAAPAQPVLL
                     PALKQTSLHPDAPRAAAPGTYADAVASAAPAVVNVYTTKEVRRRDVPVDPLYRYFFGD
                     PRGSPDRVASLGSGVIATANGYILTNNHVVQAADEIAVALADGRQFDARLIGADPESD
                     LAVLKVEATALPVITFGHDDALKVGDIVLAIGNPFDVGQTVTMGIVSALGRSNLGINT
                     FENFIQTDAAINQGNSGGALVDSTGHLVGINTAIFSRTGGSIGIGFAIPSTLVRQVMN
                     DLIKSGKVTRGYFGVEPEDITPEAADLLKLSRADGVVLKGVQRASPAGKAGLEPGDVI
                     VTINGQKVANSRGMLNQIAQLPPGSSASVRVLRLGKEVDVPVTVGERPTPRRLE"
     gene            complement(1253..1933)
                     /locus_tag="VLN25_08120"
     CDS             complement(1253..1933)
                     /locus_tag="VLN25_08120"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSQ10566.1"
                     /translation="MRATPPRLLLWLVLAILIHSPAPAARLQVVPDDDVELAQGYAAH
                     QAGDLARARALFRTGAERNQRVAQFNLAVMLLDGEGGPPDPKAGLQWLQKSAGGGFAR
                     AQYALGLLHERGELVPRSLTDATAWYRKAAEQGYRDAQVSLATQYFLGRGAPRDFAEA
                     ARWYERAAEQGDPGAAYIVASQYENGDGVEADTLRAFYWYSVAAGSGDAVAVLKAREM
                     REKLGASR"
     gene            complement(1963..>2696)
                     /gene="tatC"
                     /locus_tag="VLN25_08125"
     CDS             complement(1963..>2696)
                     /gene="tatC"
                     /locus_tag="VLN25_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008540479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HSQ10567.1"
                     /translation="FLTHLVELRTRLVRAALAIVIVFAALTPFMTEIFDLLSRPMMAA
                     LPVGSKLLATGVITPFMVPLKVTLFMALLIALPYVLWQVWAFVAPGLYKHEKKLAAPL
                     IASSVLMFALGMAYCYFIVFGLVFKFIASFAPASVSVSPDIEAYFGFVMGMFLAFGLT
                     FEVPIIVVLLARFGIADVQKLKQARPYVIVGAFIIAAVFTPPDVLSQLLLAIPLCLLY
                     ELGIFLARFVVKPKDADETAAQEAP"
BASE COUNT          507 a          904 c          866 g          419 t
ORIGIN      
        1 aactgaagcg acgttgatgt tcaggtcact gcagataccc catttaccgc ggaaacctga
       61 aacgatccga tgctccgacg tgtctggctg atcttcgcgc aggcaacgac gatcgggctt
      121 gccctgctgt ttgtcgtttc cacgctgcga ccggagtggc tggctcgcac gccggcggcc
      181 ccggcgcaac ccgtgctgct gccggcgctg aagcagacgt cgttgcatcc ggacgcaccg
      241 cgcgcggccg cgcccggaac gtatgcagac gccgtcgcct cggcggcgcc cgccgtcgtc
      301 aatgtctaca ccacgaagga agtgcggcga cgcgatgtgc cggtcgatcc gctgtatcgc
      361 tacttcttcg gcgacccgcg cggcagtccc gatcgggtcg caagcctcgg ctcgggagtg
      421 atcgccacgg cgaacggcta catcctgacc aacaatcacg tggtgcaggc ggccgacgag
      481 atcgcggtcg cactggcaga cgggcgccag ttcgatgcac gcctcatcgg tgccgatccc
      541 gagagcgacc tggcggtcct gaaggtcgaa gccaccgcac tgccggtgat cacgttcggg
      601 cacgacgacg cgctgaaggt gggcgacatc gtgctggcga tcgggaatcc tttcgacgtc
      661 ggacagacag tcacgatggg tatcgtctcc gcactcggac gctcgaatct cggcatcaac
      721 actttcgaga acttcatcca aaccgatgca gcgatcaacc agggcaattc gggcggcgca
      781 ctggtcgatt cgaccggcca tctggtcggc atcaacaccg cgatcttctc gcgcactggc
      841 ggctcgatcg gcatcggctt cgcgattccc tccacactgg tgcgccaggt gatgaacgac
      901 ctgatcaagt ccggcaaggt gacacgtggc tacttcggcg tcgagccgga ggacatcacg
      961 ccggaggctg ccgaccttct caagctgtcg cgggccgacg gcgtcgtgct caagggcgta
     1021 cagcgcgcga gtcccgccgg caaggccggt ctggagccgg gcgacgtgat cgtgacgatc
     1081 aacgggcaga aagtggcgaa cagccgcggc atgctgaacc agatcgcaca gttgccgccg
     1141 ggctccagcg ccagcgtgcg cgtgctgcga ctgggcaagg aagtcgatgt gcccgtcact
     1201 gttggagagc ggccgacacc ccgtcgactc gagtagctct gcgcgcggtg cgtcagcgcg
     1261 acgcgcccag tttctcgcgc atttcacgcg ccttgagcac ggcaaccgca tcgccgctgc
     1321 cggcggcaac gctgtaccag tagaacgcac gcagggtgtc ggcctcgacg ccatcgccat
     1381 tttcgtactg gctggccacg atgtacgccg cgcccgggtc gccctgttcg gccgcgcgtt
     1441 cgtaccagcg ggccgcctcg gcgaagtcac gcggcgcgcc gcggccgagg aaatactggg
     1501 tggcgaggct cacctgcgcg tcgcggtagc cctgctccgc cgccttgcgg taccaggctg
     1561 tcgcgtcggt cagggaccgc ggcacgagtt ccccgcgttc atgcagcagc ccgagggcgt
     1621 actgcgcgcg cgcaaagccg ccaccggcag acttctgcag ccattgcagt cctgctttcg
     1681 gatcgggcgg accaccctcg ccgtccagca acatcaccgc caagttgaac tgtgcgaccc
     1741 gctgattgcg ctcggcgccc gtgcgaaaca gtgcccgcgc acgcgccagg tcgcccgcct
     1801 gatgtgcagc gtaaccctgg gccagttcga cgtcgtcgtc cggaacgacc tgcagccgcg
     1861 cggccggggc cggcgagtgg atcaatatcg ccagcaccag ccacagcagc agtcgaggtg
     1921 gtgtggcccg catgcgctac tggtctggcc gcggtggacc catcacggag cttcctgcgc
     1981 cgcagtctcg tcggcatcct tgggtttgac gacgaagcgg gccaggaaga tgccgagttc
     2041 ataaagcagg cacagcggga tcgcgagcag cagttgcgac aacacgtccg gcggcgtgaa
     2101 cacagcagca atgatgaagg ccccgacgat gacgtagggc cgcgcctgct tgagcttctg
     2161 gacgtctgca atgccaaaac gcgccagcag cacgacgatg atcggtactt cgaaggtcag
     2221 gccgaaggcg aggaacatgc ccatcacgaa cccgaagtag gcctcgatgt ccggcgagac
     2281 cgacaccgag gcgggcgcga aactcgcgat gaacttgaac accagaccga acacgatgaa
     2341 atagcagtac gccatgccca gcgcgaacat cagcacgctt gaggcgatca gcggcgccgc
     2401 cagcttcttc tcgtgcttgt acagccccgg cgccacgaag gcccacacct gccagagcac
     2461 ataaggcagt gcgatcagca gcgccatgaa caatgtcacc ttcagcggca ccatgaacgg
     2521 cgtgatgacg ccggtcgcca gcagcttgga gccgaccggc agcgccgcca tcatcggccg
     2581 cgacagcaga tcgaagatct ccgtcatgaa gggtgtcaac gccgcgaaca cgatgacgat
     2641 tgccagcgcg gcgcggacca gccgcgtacg cagttcgacc aggtgcgtca ggaaac
//
LOCUS       DATIRT010000359         4631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_548137, whole genome shotgun sequence.
ACCESSION   DATIRT010000359 DATIRT010000000
VERSION     DATIRT010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548137"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /locus_tag="VLN25_08130"
     CDS             complement(<1..1079)
                     /locus_tag="VLN25_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HSQ10568.1"
                     /translation="MAQALTRDTVIGVIGAGAMGAGIAQVAAQAGHRVLLADAQPGAV
                     DRAAAAMVQGLQSLADRGKLSAEEARAVPARIQPSELMEFAACGLVIEAIVEDLDVKH
                     ALLTQLEAIVGHDCILATNTSSLSITAIAAGLAHPQRVVGMHFFNPATRMKLVEVISG
                     VETTPEVAETVFATAEAWGKKAAYAKSTPGFIVNRIARPFYGEAWRAYTEGAAEPATI
                     DAIVRDCGGFPMGPFELMDLIGHDVNFAVTKSVFDATFGERRYAPSLAQQELVRAGRL
                     GRKSGRGVYDYREGATRPTPRVESGAEPVQRVVAVGSLGIATPIIARLERAGIDVVRV
                     QGPRAAQEVSSGWLEIGAARLMMTDG"
     gene            complement(1082..1879)
                     /gene="paaG"
                     /locus_tag="VLN25_08135"
     CDS             complement(1082..1879)
                     /gene="paaG"
                     /locus_tag="VLN25_08135"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HSQ10569.1"
                     /translation="MAFTFESIRLDRAEGVATLTLNRPDRLNSFTVAMHGEVRAALDA
                     VDADASVRCLVLTGAGRGFCAGQDLADRAVAPGSDALDLGASIENYYKPLLLRLRNLR
                     MPTLCAVNGVAAGAGANIPFACDLVLAARSASFIQSFSKLGLVPDCGGTWWLPRLVGP
                     ARAMGLTLLGDKVPAAQAEDWGLIWRCIEDDELMPTVAALARQLAAGPTQGYVKTRQA
                     IDAALGLSFADALNVERDAQRELGRSDDYREGVTAFMEKRPPRFRGH"
     gene            2082..3746
                     /gene="paaN"
                     /locus_tag="VLN25_08140"
     CDS             2082..3746
                     /gene="paaN"
                     /locus_tag="VLN25_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaN"
                     /protein_id="HSQ10570.1"
                     /translation="MTHPLFEKHRALLDGALDAIRTRGYWSAFPEQPSPKVYGETASE
                     DGKAAALGHFGKHFDLDQPGRLGWAATEKSPYGVKLDIGYPACDPQALIDAGLAAMPA
                     WQQLGVEGRTGILLEALVRFNRASFEIAHAVMVTTGQGWMMAFQAGGPHAQDRALEAV
                     AYAYREMSFVPGSARWEKPQGKNSPMVMEKRFEVVGRGTALVIGCGTFPTWNTYPGLF
                     AALATGNPVIVKPHPNAVLPAAIGVWILREVLRENGIDPNLLTLAVIEDPKVTQALAM
                     NPAVKSIDFTGSNAFGRWLVDHARQAQVYAELAGVNNVVIESTDSYKAMLRNLAFTLS
                     LYSGQMCTTTQDLFVPAGGIDTDEGHKSFDQVATDLGLAIDKFLSDPATATAVLGAIQ
                     SPDTLARIDEVSRLADVVLASRRIDNPEFPAAQVRAPVLLKCDAGNEAAYMQERFGPI
                     SFVVKVPDALAAVALSERIVKEHGALTVGLYSTRQEIIDAMTAATLRAGVALSVNLTS
                     NVFVNQSAAFSDFHATGANPSANASYTDSAFVANRFRIIQRRWHTS"
     gene            3865..4473
                     /locus_tag="VLN25_08145"
     CDS             3865..4473
                     /locus_tag="VLN25_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HSQ10571.1"
                     /translation="MPCFEIDGVIPVVDPTAFVHPTATLIGDVIVGPGCYVAPHASLR
                     GDFGRIILERGSNVQDCCVMHGFPSTDTVVEEDGHIGHGAILHGCVVKKNAMVGMNAV
                     VMDEAVIGESAIVAACAFVKAGMVIPPRALAVGTPAKVIRILSDEEVSWKVSGTRSYQ
                     DLTRRSLHTLREVPPLAAVEAGRKRIAIEVDGVVPLVATKRD"
     gene            complement(4470..>4631)
                     /locus_tag="VLN25_08150"
     CDS             complement(4470..>4631)
                     /locus_tag="VLN25_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2783 domain-containing protein"
                     /protein_id="HSQ10572.1"
                     /translation="DDFYQALIDAHAGLTDAQSAALNARLILLLANQVGDLDTLRLAI
                     AAAREGLDR"
BASE COUNT          778 a         1583 c         1525 g          745 t
ORIGIN      
        1 ccgtcggtca tcatcaggcg cgcggcgccg atctccagcc agccggacga aacctcctgc
       61 gcggcgcgcg ggccctgcac gcgcacgacg tcgatgccgg cgcgttccag ccgcgcgatg
      121 atcggcgtgg cgatgccgag cgatccgacc gcgactacac gctgcaccgg ttcggcgcct
      181 gactccacgc gcggcgtcgg ccgcgtcgct ccctcgcgat agtcgtagac gccgcggccg
      241 ctcttgcggc ccagcctgcc ggcgcgtacg agctcctgct gcgccagcga cggcgcgtag
      301 cggcgctctc cgaatgtcgc gtcgaacacc gacttggtga ccgcgaagtt cacgtcatgg
      361 ccgatcagat ccatcagctc aaacggtccc atcggaaaac cgccgcagtc gcgcacgatc
      421 gcgtcgatgg tggccggttc ggccgcaccc tcggtgtacg cacgccaggc ctcgccgtag
      481 aagggtcgtg cgatgcggtt gacgatgaag cccggcgtcg atttggcata cgccgccttc
      541 ttgccccacg cctcggcggt ggcgaagacg gtctcggcaa cctcgggcgt cgtctcaacg
      601 ccgctgatca cttcgacgag cttcatgcgc gtggccggat tgaagaagtg catgccgacg
      661 acgcgctgcg ggtgcgcgag tccggcggca atcgcggtta tcgacagcga ggaggtgttg
      721 gtcgcgagga tgcagtcgtg cccgacaatc gcttcgagtt gcgtcagcaa cgcgtgcttg
      781 acgtccaggt cctcgacgat cgcctcgatg acgagcccgc aggcggcgaa ctccatcagc
      841 tcggacggct gaatgcgcgc cggcaccgca cgcgcttcct cggcactcag cttgccgcgg
      901 tccgccagcg attgcagccc ctgcaccatc gctgcggcag cacgatcgac ggcgcccggc
      961 tgcgcgtcgg caagcaggac acggtgccct gcctgcgcgg ccacctgcgc gatgcccgcg
     1021 cccatcgccc ccgcgccgat gacgccgatg accgtgtccc tcgtgagcgc ctgcgccatt
     1081 gtcagtggcc cctgaagcgc ggcgggcgct tctccatgaa cgccgtaacg ccctcgcgat
     1141 agtcgtccga ccggccgagt tcgcgctgcg cgtcgcgctc gacattgagt gcatccgcga
     1201 acgaaagtcc cagcgccgca tcgatcgcct ggcgtgtctt cacgtacccc tgcgtcggcc
     1261 ccgcagccag ttggcgcgcg agtgctgcaa cggtcggcat cagttcatca tcctcgatgc
     1321 agcgccagat caggccccag tcctcggcct gtgcggcagg caccttgtct cccagcagcg
     1381 tcagccccat cgcgcgcgcc ggaccgacca gccgcggcag ccaccaggtg ccgccgcaat
     1441 ccggcaccag cccgagcttg gagaacgact ggatgaagct cgcggaccgg gcggcaagca
     1501 ccaggtcgca cgcgaacgga atgttcgcgc cggcgccggc cgccacgcca ttgaccgcgc
     1561 agagggtcgg catgcgcagg ttgcgcaggc gcagcagcag cggtttgtag tagttctcga
     1621 tcgacgcgcc gaggtcgagc gcgtcactgc cgggtgcgac ggcacgatcc gcaagatcct
     1681 ggccggcgca gaatccgcgc ccggcaccgg tcagcacgag acagcggacc gaggcgtccg
     1741 cgtcgaccgc atcgagggcc gcacgtacct cgccatgcat ggccacggtg aagctgttca
     1801 gccggtcggg ccggttgagc gtcagggtcg ccacgccctc cgccctatcg aggcggatgg
     1861 actcgaacgt gaatgccatc gggagctgtc tccgtgatcg ggtccgatgt cagcgcgcat
     1921 ctactgagcg cgcgccggat tcgttgaccg accgttcaat caatagtagc tttgccggtt
     1981 cactgctgac aagtcatgct tgccattctc acgcgatcca gttcgcgcgg ccggagcagg
     2041 acgtcaccgc gccgcaccac cgcccaacac aggagacgga gttgacccac ccactgttcg
     2101 agaagcaccg cgcattgctg gacggcgcgc tcgacgccat ccgcacgcgc ggctactggt
     2161 cggcgtttcc ggagcagccg agccccaagg tctatggcga gacggcaagc gaggacggca
     2221 aggcggccgc actcggacac tttggcaagc acttcgacct ggaccagcca ggccggctcg
     2281 gatgggccgc gacggagaaa tcgccctacg gcgtgaagct cgacatcggc tatccggcgt
     2341 gcgatccgca ggcattgatt gatgcgggcc tcgcggcgat gcccgcatgg cagcaactcg
     2401 gtgtcgaagg acgcaccggc atcctgctcg aagccctggt ccgcttcaac agggccagct
     2461 tcgagatcgc gcacgcggtg atggtcacga ccggacaagg ctggatgatg gcgttccagg
     2521 ccggtggacc gcacgcgcag gaccgggccc tcgaagccgt cgcctatgcc taccgcgaga
     2581 tgagcttcgt gcccggcagc gcgaggtggg agaaaccgca gggcaagaac tccccgatgg
     2641 tgatggagaa gcgcttcgag gtcgtcgggc gcggtaccgc gctggtgatc ggttgcggca
     2701 cgtttccaac gtggaacacc tacccggggc tttttgccgc cctggccaca ggcaacccgg
     2761 tcatcgtcaa gccacacccg aatgccgtgc tgcccgccgc gatcggcgtg tggatcctgc
     2821 gcgaggtcct gcgcgagaac ggtatcgatc ccaacctcct gaccctcgcg gtcatcgagg
     2881 acccaaaggt cacgcaggcg ctcgccatga atccggccgt gaagtcgatc gacttcacgg
     2941 gcagcaatgc gttcggacgc tggctggtcg accatgcgcg ccaggcacag gtctatgccg
     3001 aactcgccgg cgtcaacaac gtcgtgatcg agtcgaccga cagctacaag gcgatgctgc
     3061 gcaacctcgc gttcacgctc agcctgtact cgggtcagat gtgcacgacg actcaggatc
     3121 tcttcgtccc ggccggcggc atcgacaccg atgaaggcca caagagcttc gaccaggtcg
     3181 ccaccgacct tggcctggcg atcgacaagt tcctctccga cccggcgacc gcgacggccg
     3241 tgctcggtgc gatccagtcg cccgatacgc tggcgcggat cgacgaggtc tcccggctgg
     3301 ccgacgtggt gctggcatcg cgccgcatcg acaatccgga gtttccggct gcccaggtgc
     3361 gcgcaccggt gctgctcaag tgcgacgccg gcaacgaggc tgcctacatg caggagcgct
     3421 tcggcccgat cagtttcgtc gtcaaggtgc cggacgcgct cgcggccgtt gcgctatccg
     3481 aacgcatcgt gaaggaacac ggcgcactga cggtggggct gtactcgacc cggcaggaga
     3541 tcatcgatgc gatgaccgca gcgacgctac gagccggagt ggcactgtcg gtcaacctga
     3601 ctagcaacgt gttcgtgaac cagtccgccg cgttctcgga cttccacgca accggcgcca
     3661 acccttccgc gaatgcgagc tacaccgact cggcatttgt ggcgaaccgc ttccggatca
     3721 tccagaggcg ttggcataca agttaggcat cgctcgtgta cgcgagcctc tggctcgcac
     3781 gtgcactcgc gccgattgag ttaagcggtc gggcttcggg cggccgtgcg cccgaccgca
     3841 gtacgactgc ttgctggagg cgttatgccg tgtttcgaaa ttgatggtgt gattccggtc
     3901 gtcgatccga cggcgttcgt gcatccgacg gcgacgctga tcggcgatgt catcgtcggg
     3961 cccggctgct acgtcgcacc gcacgcgagc ctgcgcggcg acttcggccg catcattctc
     4021 gagcgcggca gtaatgtgca ggactgctgc gtgatgcacg gctttccgag taccgacacc
     4081 gttgtcgagg aagatggcca catcggccac ggcgcaatcc tgcacggctg cgtcgtgaag
     4141 aagaacgcga tggtcggcat gaacgccgtc gtcatggacg aggcggtgat cggcgagtcg
     4201 gccatcgtcg cggcctgcgc gttcgtcaag gccggcatgg tcatcccgcc gcgcgcgctc
     4261 gcggtcggca cgcccgccaa ggtgatccgg atactgtctg acgaggaagt gagctggaag
     4321 gtcagcggca cgcgcagtta ccaagacctg acacgtcgca gcctgcacac actgcgcgaa
     4381 gtaccgccgc tcgctgcggt ggaggccggc cgcaagcgga tcgcgatcga ggtcgacgga
     4441 gtcgtgccgc tggtggcgac gaagcgcgac tagcggtcca gcccctcgcg cgccgcggct
     4501 atcgccaggc gcaacgtatc cagatcgccg acctgattgg ccagcagcag gatgaggcgt
     4561 gcgttcagtg cggcgctctg cgcgtcggtg agtcccgcgt gtgcgtcgat cagcgcctgg
     4621 tagaagtcgt c
//
LOCUS       DATIRT010000360         4093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_746219, whole genome shotgun sequence.
ACCESSION   DATIRT010000360 DATIRT010000000
VERSION     DATIRT010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746219"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /locus_tag="VLN25_08155"
     CDS             complement(<1..1079)
                     /locus_tag="VLN25_08155"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HSQ10573.1"
                     /translation="MTATEPQVATGPSSVAMPKTIGRFTILNEIGRGSYGVVWAAQDP
                     VLVREVAIKVIPLAREAEFRTQIEANFLREAKSAGGMSHPSIVTIYDAGKTDAFAYIA
                     MERLHGQDLHEYLAGGNRMSPRQAASMMMRLADAIHYANKRGLVHRDIKPSNIFLSRD
                     LKPKLLDFGTALAPTPRDGKDKGTRQLVGTPNYMSPEQAKGEPLDARSDIFSLGTILY
                     ELLTGRRAFDGRTIDETLEQVLSARPRAVDKIRPETPEPLLAIVRKAMERDPANRYQK
                     ASDFRNALADFVDGSRAPGADLSAATRPADFTPPPAARIGLKLVLGAAALLAAVVVIF
                     IALRPEPAPPATVVNAVPVPVTTAPP"
     gene            complement(1258..2430)
                     /locus_tag="VLN25_08160"
     CDS             complement(1258..2430)
                     /locus_tag="VLN25_08160"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSQ10574.1"
                     /translation="MRAAAVVFRKELRDALRDRRTWIVVLLSSLLAGPISLLLIASFV
                     SSAEESAARREVVIANATAAPTLVNYLQRNGATIREAPPDFREQLRSGHLQNAVVVIP
                     DDFGRRLATGETIRLDVVFDGTANRAQTAVRTTMVLLQGFNGELGSQRLLARGVSPQV
                     LKPLQIEDVNLAGSRARGAQLLFLVPWLALVGAVVGAISVAIDVTAGERERGSLEPLL
                     MNPVSTGSLVLGKWAVTATCSAAVVLLTLAGFTAAMQFVRSENLAALMQFGLPEVALF
                     IVLLLPFSAMVGSLNMLAATYGRSHKEAQTYASYITMLVNFVPILPLFLTIRDAPWQL
                     FVPALGQQTVLMRALRGEVVSTTDIVLPSAIALAITAAALLVQARLLRDERIVFSR"
     gene            complement(2427..3200)
                     /locus_tag="VLN25_08165"
     CDS             complement(2427..3200)
                     /locus_tag="VLN25_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSQ10575.1"
                     /translation="MIEARHLAKSFSARVQRRRTRVAAVVDVSFDAPDGTITALLGPN
                     GAGKTTTLRLVGTLVEADAGSITVGGLDVRRNARGVRAQLGMLSDARGLYARLTAREN
                     IVYYGDLRGIGRTALEDRLAMLSELLDMTPLLDRRTEGFSTGERMKVALARALIHDPQ
                     HLILDEPTSGLDVMSTRALRRLLVHLRSQGKCIVLSSHIMPEVEQLADRIVIVAHGRS
                     IAQGSGDEIRRRAGAATLEDAFVALAYGEEAAHAGAALR"
     gene            complement(3197..>4093)
                     /locus_tag="VLN25_08170"
     CDS             complement(3197..>4093)
                     /locus_tag="VLN25_08170"
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSQ10576.1"
                     /translation="YGTRVALEYLRRYPQHVRTAVLDGVAPPDMTLPAAFALDADAAL
                     AGLSATCRSDERCRARFPDFDARIGALLERADSGFEITVSDPLTGQPQSLQVDRRLLA
                     SLLRVPLYLPALGAVLPQALAEAEGGDYNGLVALSVVVSGRVNENFALGMHFAVICAE
                     DLPRLGPTHRKAAAATRFGTTFTDLYVDACTSIATRTVPDGFYQVPRADVPVLVLSGG
                     LDPATPPRHGASVAERLGNARHVISPHLGHGLSGQACAPRLISRFVREGDFVDLDADC
                     LEKLPAASFFEPIEAPPSKAPR"
BASE COUNT          693 a         1334 c         1419 g          647 t
ORIGIN      
        1 ggcggagccg ttgtcacggg caccgggacg gcattcacca cggtggccgg tggcgcgggt
       61 tccggacgca gcgcgatgaa gatcacgacg accgccgcga gcaacgcagc agcgccgagt
      121 acgagtttga gaccgattcg cgccgccggc ggcggcgtga agtccgcggg tcgcgtcgct
      181 gccgacaggt cggctcccgg ggcgcgcgag ccgtcgacga agtcggccag tgcgttgcga
      241 aagtccgagg ctttctggta ccggttcgcc ggatcgcgtt ccatcgcctt gcgcacgatg
      301 gccagcagcg gctccggtgt ctcgggccgg atcttgtcga cggcgcgcgg acgggcgctg
      361 agaacctgtt cgagcgtttc gtcgatggtc cggccgtcga atgcacgccg acccgtcaac
      421 agctcgtaca ggatggtgcc gagcgagaag atgtcgctgc gcgcatcaag cggttcgccc
      481 ttggcctgct cgggcgacat gtagttcggt gtgccgacca gctgccgcgt gcccttgtcc
      541 ttgccatcgc gcggggtcgg cgccaaagcg gtgccgaagt cgagcagctt gggcttgagg
      601 tcgcgcgaca ggaagatatt tgacggcttg atgtcgcgat ggaccaggcc gcgcttgttg
      661 gcgtaatgga tggcatcggc caggcgcatc atcattgacg cggcctgtcg cgggctcatc
      721 cggttgccgc ccgcaaggta ctcgtgcaag tcctggccgt gcagccgctc catcgcgatg
      781 taggcgaagg catcggtctt gccggcgtcg tagatcgtga cgatgctggg atggctcatc
      841 ccgccggcgg acttggcctc gcgcaggaag ttggcctcga tctgagtcct gaactcggcc
      901 tcacgcgcga gcgggatcac cttgatcgcg acttcgcgga ccagcaccgg atcctgcgcc
      961 gcccagacga caccatagga gccgcgaccg atctcgttga gaatcgtgaa gcgcccgatc
     1021 gtcttcggca tcgccacgga agacggtccc gtggcgacct ggggctcggt ggcagtcatc
     1081 ggctcggaca tcgtagcgcg attatcgcac ccgagcggct tctcaatcac cggaattggc
     1141 gttgctcgag gaccgaactg tgacaggtcg gcgtccggcc gggcctgatc gccattgcac
     1201 cggatctatg ggtgcctccg ccgacatcgg ctgcgccgca tcagccggac gcgcgactca
     1261 cctcgagaag acaatccgct cgtcgcgcaa cagacgcgcc tgcacgagca gggccgctgc
     1321 ggtgatcgca agtgcaatgg ccgacggcag gacgatatcc gtcgtcgaga cgacttcgcc
     1381 gcgcagggcg cgcatcagca cggtctgctg acccagcgcc ggtacgaaga gttgccacgg
     1441 cgcatcgcgt atggtcagga acagcggcag gataggcacg aagttcacca gcatcgtgat
     1501 gtagctcgcg taggtctgcg cttccttgtg gctgcggccg taggttgccg ccagcatgtt
     1561 gagcgagccc accatggccg aaaacggcag cagcagcacg atgaacagtg ccacctcggg
     1621 caggccgaac tgcatcagcg ccgccaggtt ttcgctgcgc acgaactgca tcgcggcggt
     1681 gaaaccggca agcgtgagca gcacgacggc ggccgagcag gtcgccgtca ccgcccattt
     1741 gccgagcacg agcgaaccgg tcgacaccgg attcatcagc agcggctcga gtgagccgcg
     1801 ttcgcgctcg ccggccgtga cgtcgatggc gacgctgatc gcaccgacga ccgcacccac
     1861 caatgccagc cagggcacga ggaacagcaa ctgcgcgccg cgtgcgcggc tgccggcgag
     1921 gttgacgtcc tcgatctgca ggggcttcag tacctgcggg ctgacgccgc gggccagcag
     1981 gcgttgcgac ccgagttcac cgttgaaacc ctgcaacagc accatcgtgg ttcgcacggc
     2041 cgtctgggcg cggttcgcgg tcccgtcgaa cacgacatcg aggcggatgg tttcgccagt
     2101 ggccagacgc cgtccgaagt cgtcgggaat cacaaccacc gcattctgca ggtggccgga
     2161 tcgcaattgt tcgcggaagt ccggtggcgc ttcgcggatc gtcgcaccgt tgcgttgcag
     2221 gtagttgacg agggtcggtg cggccgtggc gttggcgatg accacttcgc gccgcgcggc
     2281 gctttcttcc gcgctcgaca cgaaactggc gatcagcagc agcgagatag gaccggccag
     2341 cagcgaactc agcagcacca cgatccaggt gcgccggtcg cgcagtgcat ccctgagttc
     2401 cttgcggaaa acgacggctg cggctctcat cgcaatgccg ccccggcatg cgctgcttct
     2461 tcgccgtacg ccagcgccac gaacgcgtct tccagcgtgg ctgcgccggc ccggcgccgg
     2521 atctcgtcgc cgctgccttg cgcgatgctg cgcccgtgcg caacgatgac gatgcggtcg
     2581 gcgagttgct cgacctccgg catgatgtgc gaggacagca cgatgcactt gccctgcgag
     2641 cgcagatgga caaggaggcg tcgcagggcc cgcgttgaca tcacgtcgag cccgctggtc
     2701 ggctcgtcga gaatcaggtg ttgcggatcg tgaatcagcg cacgggcaag cgcgaccttc
     2761 atgcgctcac cggtgctgaa tccctcggtc cggcggtcga gcagcggtgt catgtcaagc
     2821 agctccgaca gcatcgccag ccggtcctcg agagcggtcc gtccgatgcc gcgcagatcg
     2881 ccgtaataga caatgttctc gcgggcggtg agtcgtgcat acaggccgcg cgcatcggag
     2941 agcattccga gctgcgcccg gacgccgcgc gcattgcggc ggacatcgag tccgccgacc
     3001 gtaatgctgc cggcatcggc ttcgacaagc gtgccgacga ggcgcagcgt ggtggtcttg
     3061 ccggcgccgt tggggcccaa cagcgcggta atcgtgccgt cgggggcgtc gaagctgacg
     3121 tcgaccaccg cagcgacccg cgtgcgccgg cgctgtacgc gggcgctgaa cgacttggca
     3181 agatgccgcg cctcgatcat cgcggcgcct tggagggcgg tgcttcgatt ggctcgaaga
     3241 agctcgcggc cggcagcttc tccaggcaat cggcgtcgag gtcgacgaag tcaccttcgc
     3301 gcacaaaacg cgagatcagt cgcggggcgc aagcctgtcc gctgagcccg tggccgaggt
     3361 gaggcgagat gacatgacgc gcgttgccga ggcgctcggc gaccgatgcg ccgtgacgcg
     3421 gcggcgtagc agggtccagg ccgccggaca gcaccagcac tggaacgtcc gcacgcggga
     3481 cctggtagaa gccatcaggg acggttcgcg tcgcgatcga ggtgcacgcg tccacataca
     3541 ggtccgtaaa cgtggttccg aagcgcgtcg ccgcagcggc tttgcggtgc gtcggaccca
     3601 ggcgcggcag atcctccgca caaatgacgg cgaagtgcat gccgagcgca aagttctcat
     3661 tgacgcgacc gctgacgacg accgacaggg cgacgagacc gttgtagtcg cctccctctg
     3721 cttcggcgag cgcttgcggc aggaccgccc ccagggccgg caggtacagc ggcacgcgca
     3781 gcagcgatgc gagcaagcgc cggtccacct gcagagactg cggttgtccg gtcagcggat
     3841 ccgacacggt gatctcgaag ccgctgtcgg cacgttcgag cagtgcgccg atgcgcgcat
     3901 cgaagtcggg aaagcgggcc cggcagcgct cgtcggaccg gcaggtcgcg gacaacccgg
     3961 ccaacgctgc atcggcgtcg agggcaaaag ctgcgggcag cgtcatgtcc ggcggcgcca
     4021 cgccgtccag cacggcggtg cgaacgtgct gcgggtagcg gcgcaggtac tcgagtgcca
     4081 cgcgcgttcc gta
//
LOCUS       DATIRT010000361         1617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1238768, whole genome shotgun sequence.
ACCESSION   DATIRT010000361 DATIRT010000000
VERSION     DATIRT010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238768"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VLN25_08175"
     CDS             <1..497
                     /locus_tag="VLN25_08175"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSQ10577.1"
                     /translation="EFRGLPETEARRQAEDWLKRVGLGGFGDRYPHQLSGGMRKRTSL
                     AQTLALDPDIILMDEPFSALDVQTRQLMENEVLALWAEKKKAVLFITHDLDEAIAMSD
                     RVVVLSAGPASHPIGEFVIDLARPRDVAEVRVTPRFIDLHKANWNVLREEVLKGYAQQ
                     KQAA"
     gene            505..1332
                     /locus_tag="VLN25_08180"
     CDS             505..1332
                     /locus_tag="VLN25_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSQ10578.1"
                     /translation="MWNAIRPNTKNLRFWQVGVLVALFLLWHVLTKPGLVPPLFFDGE
                     NDAKFFFGEPVKILERIWTWFVVDRDIYQHLWVTLLETVLAFLIGTVSGLVMGMWLAL
                     SPVAAAILDPYIKAMNSMPRVILAPIFAVWFGLGIASKVALGFTLVFFIVFFNVYQGV
                     KEVSPTVLANARMLGATRRQLLRHVYLPSATSWVFSSLHTSVGLAFVGAVVGEYLGSS
                     RGVGYLILQAEGTFDINTVMAGILVLTGFALVLDGIVGRVEKHLMKWQPRAGETEKL"
     gene            1412..>1617
                     /locus_tag="VLN25_08185"
     CDS             1412..>1617
                     /locus_tag="VLN25_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10579.1"
                     /translation="MEAIYEYANQQAAEPFWQRIQKFFLLPLDRSVLLRIGGLSAALV
                     VSLVLLLFGPFGTVLIAAAAFALLV"
BASE COUNT          254 a          506 c          525 g          332 t
ORIGIN      
        1 tggagttccg cggcctgccc gagaccgagg cgcgccgcca agccgaggac tggctcaagc
       61 gcgtgggcct gggcggcttc ggcgaccgct acccgcacca gctgagcggc ggcatgcgca
      121 agcgcacctc gctggcgcag accttggcac tcgacccgga catcatcctg atggacgagc
      181 ccttctcggc gctggatgtc cagacgcggc agctgatgga aaacgaggtc ctggccttgt
      241 gggcggagaa gaagaaggcg gtgctcttca tcacgcacga cctggacgag gcgatcgcga
      301 tgagcgaccg cgtcgtcgtg ctgtcggccg ggccagcgtc gcatccgatc ggcgagttcg
      361 taatcgacct cgcacgcccg cgcgatgtgg ccgaggtgcg ggtgaccccg cgcttcattg
      421 acctgcacaa ggcgaactgg aacgtgctgc gcgaggaagt actgaagggc tacgcgcagc
      481 agaagcaggc cgcgtgagcg gataatgtgg aacgcgatcc gccccaatac gaagaacctg
      541 cgcttctggc aggtgggcgt gctggtcgct cttttccttc tctggcacgt cctgaccaag
      601 cccggactgg tgccgccgct gttcttcgat ggcgagaacg acgccaaatt tttcttcggc
      661 gagccggtca agatcctcga gcgcatctgg acctggttcg tcgtcgaccg cgacatctac
      721 cagcacctgt gggtgaccct gctcgagacc gtgctggcct tcctgatcgg caccgtttcc
      781 gggctggtga tgggcatgtg gctggcgctc tcgccagtgg ctgccgcgat cctcgacccg
      841 tacatcaagg cgatgaactc gatgccgcgc gtgatcctgg cgccgatctt tgccgtctgg
      901 tttggcctcg gcattgcttc caaggtcgcg ctcggcttca ctctggtgtt cttcattgtc
      961 ttcttcaacg tctaccaggg tgtcaaggaa gtcagcccga cggtgctcgc gaatgcacgc
     1021 atgctcgggg ctacgcgccg acagctgttg cgccacgtct acctgccgag cgccaccagc
     1081 tgggtgtttt cctcgctcca cacgtcggtg ggcctggcgt tcgtcggtgc ggtggtcggt
     1141 gaatacctcg gctcctcgcg gggcgtgggc tatctgatcc ttcaggccga aggcactttc
     1201 gatatcaata ccgtgatggc cggcatcctg gtgctgacgg gcttcgcgct ggtgctcgac
     1261 gggatcgtcg ggagggtcga aaagcacctg atgaagtggc agccgcgcgc cggagagacg
     1321 gaaaagctgt agttttccag ccgaagctcg gcgtttggcg tcgtgagcgg ggtttaccct
     1381 cgatagactt tcgtattcgg tccacgcggg gatggaagcc atttacgaat acgccaacca
     1441 gcaggcggca gagcctttct ggcagcgcat ccagaaattc tttctgctac cgctcgaccg
     1501 ttccgtcctg ctccgcatcg gcgggctgtc agcggcgctg gtcgtctcgc tggtactgct
     1561 cctcttcggt ccgttcggca cggtactgat tgccgctgcc gcattcgcgc tgctcgt
//
LOCUS       DATIRT010000362         3521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_156191, whole genome shotgun sequence.
ACCESSION   DATIRT010000362 DATIRT010000000
VERSION     DATIRT010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156191"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VLN25_08190"
     CDS             <1..591
                     /locus_tag="VLN25_08190"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSQ10580.1"
                     /translation="PEVTEQFDTIKQSVLANAYVEDFIKNNPISDDMVKAEYERITAT
                     ITGTEYKARHILVEKEAEAKEIIARLRKDPGAFAKLAMEKSKDSGSKARGGELGWFDP
                     KRMVPEFGAAVSTLEKGKFTDAPVKTQYGYHVILLEDSRKIEAPPLEDVKAQISQQLQ
                     QQSVKKQLDALKAAAKVEVVGAQASEPSTPDASKSK"
     gene            complement(795..2420)
                     /locus_tag="VLN25_08195"
     CDS             complement(795..2420)
                     /locus_tag="VLN25_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HSQ10581.1"
                     /translation="MTPAAWLGVSLALPLAMLLACLWPAARDRMLPWLAWAPLPALLA
                     ALGVEREAPLVVIAVGAYRGALVLDAPGALLLGVAALLWSAAGAYAATYQRGQPNRGR
                     FAAGWLLCLTGCIGVFLAADMPLLYLMMATMTLGAGALVFQDESAGARRAAGIYLGLA
                     LAGETLVLIAMILLVQAMPDGSLLIRDAAAALPTSRQRDLIVVLLLIGFGLKAGLVPL
                     HVWMPLAHAAAPMPASAVLSGVVVKAGIIGLIRFLPVDAALADWGNTLALVGMFTALY
                     AVAIGITQSHPKVVLAYSSVSQMGFTAAVIGMGIVNLETATPVLAAFYATHHILVKGA
                     MFLLVGVVAATAATRLRGTLLLAIVLALGLGGLPLTGGALAKLAVKGPLGSGWAETVG
                     YASAIGTTLLMLHFVRRVATVHATAVDARAPAGLHWPWLVTAIAAIVVPWVLYLVLSL
                     GAVAEILAPKALWASLWPVAVGAALAWMLARAGDRLPRVPPGDIAVVIGAAAPSAARA
                     SLGLARLDVVLRQWAVAGVSMLAIAALFGAALY"
     gene            complement(2417..>3521)
                     /locus_tag="VLN25_08200"
     CDS             complement(2417..>3521)
                     /locus_tag="VLN25_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HSQ10582.1"
                     /translation="VFLAGDFFTLYVALELVAFAAVPLVSLDGRAETLQAALRYLLFA
                     LAGSVLYLVGTVLIYGGYGTLDIALLSQQARADAVTIVALALMTVGLLAKTALFPLHL
                     WLPPAHAGAPAAASALLSGLVIKGSYFLVIRLWLDAFGSVVAPAAAQLVAAFGAGAII
                     IGSVVALRQARLKLMVAYSTVAQIGYLFLMFPLVLGAASAGAAATALAGGLLQAISHA
                     TAKAAMFMSAGLVYAALGHDRVADLRGAARALPMTIAAFALSGIALIGLPPAGGFLAK
                     WLLLSSALESSQWWWVVVIAIGGLLTAAYVFLVLSKAMTTPEAPITVVPVSRAREGVV
                     LALAILSAVLGVAALAPLDLVLIGRDGMTGATR"
BASE COUNT          742 a         1241 c         1117 g          421 t
ORIGIN      
        1 cccgaggtca cggagcagtt cgacaccatc aagcaatccg ttctggccaa tgcctacgtc
       61 gaggatttca tcaagaacaa tccgatcagc gacgacatgg tgaaggcgga atacgagcgc
      121 atcacggcaa caatcactgg cacggaatac aaggcgcgcc acatcctggt ggagaaggaa
      181 gccgaagcca aggagatcat tgccaggctg agaaaggatc caggcgcatt tgcgaaactg
      241 gcgatggaaa aatcgaagga ttcgggctcc aaggcccgtg gcggcgaact gggatggttc
      301 gatccaaaaa gaatggttcc ggaattcggt gcggccgtga gcacgctgga aaagggcaag
      361 ttcaccgacg cgccggtcaa gacccagtac ggttatcacg tcatcctgct tgaagattca
      421 aggaagatcg aggcgcctcc gctggaagac gtcaaagctc agatcagcca gcagcttcag
      481 cagcaaagcg tcaagaagca actggacgcg ctcaaggccg cggcgaaggt cgaagtcgtt
      541 ggcgctcaag cttcggaacc ttcgacaccc gatgcttcaa aatcaaagtg aacgacccgg
      601 cctcgtgaac tgctgaattg ccgcgactcg agctgaactg acatagcgtt cgtagaagcg
      661 cctgcgaatc gaatgaaagg cagggcatgt ccgctttcag cgattgcaga cgacggcttg
      721 cggtcggccg aggtcccggt gtccatccgc cgatctgccg tggccctttc ctgaccagta
      781 ctggagcgaa tcgctcagta caaggcggcg ccgaacaagg ccgcaatcgc cagcatcgat
      841 acgccggcca cagcccactg ccgcagtacg acgtcgagtc gcgccagacc aaggctcgct
      901 cgcgcggcag acggcgccgc ggcaccgatc accacggcga tgtcacccgg cggcacccgt
      961 ggcagtcggt cacccgcgcg tgccagcatc caggcgagcg cggcaccgac tgcgactggc
     1021 cacagtgagg cccacagcgc cttcggcgcc aggatttcgg caaccgcgcc gagcgacagc
     1081 acgagataca agacccacgg cacaacaatc gccgcgatcg ctgtcaccag ccacggccag
     1141 tgcaatccgg ccggcgcgcg cgcgtcgacc gccgtggcgt gaaccgtcgc cacgcggcgg
     1201 acgaagtgca gcatcagcag tgttgttccg atcgcggagg catagcccac ggtctccgcc
     1261 cagccgctgc ccagtggccc cttgaccgcg agcttggcca gggcgccgcc ggtcagcggc
     1321 aacccgccga gccccagcgc cagcacgatg gcgagcagca gcgtgccacg caggcgcgtg
     1381 gcggcggtcg ctgccaccac gccgaccagc aggaacatcg ctcccttgac caggatgtga
     1441 tgggtggcat aaaacgccgc cagcaccggc gtggccgtct cgagattcac gatgcccatg
     1501 ccgatcactg cggcggtgaa acccatctgg ctgacgctcg aatacgccag caccactttc
     1561 ggatgcgact gcgtaatgcc gatcgccacc gcatacagcg cggtgaacat tccgaccagg
     1621 gccagcgtgt tgccccagtc agccagcgcc gcgtcgacag gaaggaaacg gatcaggccg
     1681 atgatgccgg ccttgacgac gacgccgctc agaacggccg acgcgggcat cggtgcggcc
     1741 gcgtgcgcca gcggcatcca cacgtgcagc ggcaccaggc cggccttcag tccgaagccg
     1801 atcagcagca gcacgacgat caggtcgcgc tgccgcgagg tcggcagcgc cgccgcggca
     1861 tcgcggatca gcaggctgcc gtccggcatc gcctgcacga gcaggatcat cgcgatcagc
     1921 accagcgtct cgccggcaag cgccagcccg agatagatgc cggcagcgcg acgcgcaccg
     1981 gcgctctcgt cctggaacac cagcgcgccc gcgccgagtg tcatcgtcgc catcatcaga
     2041 tacagcagcg gcatgtccgc ggccaggaac acgccgatgc agccggtcag gcaaagcagc
     2101 cagccagcgg caaaacggcc acgattcggc tggccgcgct ggtacgtcgc ggcgtaggcg
     2161 ccggccgcgc tccacagcag cgccgccacg cctagcagca gcgccccggg cgcatcgagg
     2221 acgagcgcac cgcgataggc tccaacggcg atgaccacca gcggtgcctc gcgctcgaca
     2281 ccgagcgcag cgagcagtgc cggcaggggc gcccaggcca gccacggcag catgcgatcg
     2341 cgcgcggccg gccacaggca cgcgagcagc atcgccagcg gcagggccag cgacactccg
     2401 agccaggcgg ccggggtcat cgcgtcgccc cggtcattcc atcgcgcccg atcagcacca
     2461 ggtccagcgg cgccagcgcc gccaccccca gcaccgcgga caggatcgcc agcgcaagca
     2521 cgacgccttc gcgtgcgcgc gacaccggca ccacggtaat cggcgcctcg ggcgtcgtca
     2581 tcgccttcga cagcacgagg aacacgtacg ccgccgtcag caacccaccg atcgcgatca
     2641 caacgaccca ccaccactgc gacgactcga gcgccgacga cagcagcagc cacttggcga
     2701 ggaagcctcc agccggtggc aggccgatca acgcgatgcc gctgagtgcg aacgcagcga
     2761 ttgtcatcgg cagcgcacgc gcggcgccac gcaggtcagc gacacgatcg tggcccaggg
     2821 cggcgtagac cagaccggcc gacatgaaca tcgccgcctt cgcagtcgcg tgcgagatcg
     2881 cctgcagcag tccgcccgcc agcgccgtgg ccgcggcacc ggccgacgcg gcgccgagca
     2941 ccagcggaaa catcaggaat aagtagccga tctgcgccac cgtcgagtag gccaccatca
     3001 gcttcaggcg cgcctgcctc agagcgacca cgctgccgat gatgatggca cccgcaccaa
     3061 acgccgccac gagctgcgcc gcggcgggtg caacaacgct gccgaaggca tcgagccaca
     3121 gccggatcac caggaagtac gagcctttga tcacgaggcc cgaaagcagc gcgctcgccg
     3181 ccgccggcgc gccggcgtgc gccggcggca gccacaggtg cagcgggaac agtgcggtct
     3241 tggccagcaa gccgactgtc atcagcgcga gtgcgacgat ggtcaccgca tcggcccgag
     3301 cctgctgcga gagcagcgcg atgtcgagcg ttccatagcc gccgtagatc agcaccgtgc
     3361 cgaccaggta cagcactgag cccgccagcg caaacagcag gtaacgaagc gccgcctgca
     3421 aagtctcggc acgtccgtcg aggctcacca gcggcaccgc cgcgaacgcc accagttcga
     3481 gcgcgacata cagcgtgaag aagtcgccgg ccaggaacac c
//
LOCUS       DATIRT010000363         4092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_255350, whole genome shotgun sequence.
ACCESSION   DATIRT010000363 DATIRT010000000
VERSION     DATIRT010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255350"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="VLN25_08205"
     CDS             complement(<1..649)
                     /locus_tag="VLN25_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HSQ10583.1"
                     /translation="MTRRFDLVCIGRAAVDLYGEQIGASLQEVSTFARYLGGSPANTA
                     VGCARLGLRPAMLTRVGDEQNGEFVRNELARAGVDVTHVVADPRHLTALVFLSIRDEN
                     TFPLLFYRDRCADMALTVDDVDDSLIASANALLISGTHLSQESTRAACRKAIAAAVAA
                     GTRVALDIDFRPVLWGLRPLGDGATRYVESSIVTDTLHEFLSSCDLIVGTEEEFAI"
     gene            complement(646..1527)
                     /locus_tag="VLN25_08210"
     CDS             complement(646..1527)
                     /locus_tag="VLN25_08210"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HSQ10584.1"
                     /translation="MQSATVRTHGTRRERTAGFEPLMAEIAGRLEALPRQLRSIAQLA
                     LDAPETFAFGTAAELAQAAGVQPSALVRFAAALGFDGFAALRQVFRSHLVAHAPSYRE
                     RIQKLRRSTAAATAGDVVRMQVDEAIGGLQHLTKTLDERKLDAAVDLLAKSGHVYLLA
                     ARRSFPVVSYLAYALAQLERRVTLLDGIGGMNAQLAAALAPRDLLLVVSFRSYTPEVI
                     DIARQAHGRGAGVLGITDSRASPLVPCSTLAILLGDDRRLPFRSLVEPIVIAQSLVIA
                     LGHRLTETPARRNGTRR"
     gene            1633..2688
                     /locus_tag="VLN25_08215"
     CDS             1633..2688
                     /locus_tag="VLN25_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSQ10585.1"
                     /translation="MPPQPVVETSMSAVPTGLPRGPVSLQAARQGFAGPLALTLLVLV
                     TTPLWIKEIGLYQYIAVEIVIWALFALSYNLLLGQAGLPSFGHGAFFGVGAYAFGLAV
                     KHVAPDLWLGLGAALAAAALAGALVAAFISHRRGIYYALMTIAFGQVLWFVAIKWHSV
                     TGGEDGLLGIPRPPVDLGIIELSLNSNEALFYFAVVLFTLVIAFLWQLQDTPFGRVMA
                     AVKGNEERARYCGHAVWRIKFTSFVLSAALAGLAGALFAMAQNSAYPNVMSLHNSGFV
                     VMMVLIGGGLVSFWGPVIGAAFFILARDLLGATTEAWLLWYGLLFMAVVLFKPEGIVG
                     AWRMLRQRAMRASAGSH"
     gene            2688..3431
                     /locus_tag="VLN25_08220"
     CDS             2688..3431
                     /locus_tag="VLN25_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSQ10586.1"
                     /translation="MALLEARELHQRFGRQIVLDGVSLSFEEGSLSGIIGPNGAGKTT
                     CFNVLTGRYKPDRGSVHFDGDDITGRSPAAIARRGIARSFQLMNLFDALAAIDNVIVA
                     MPPLRRRAFQPFGAVRRDIELHARAMALLADVGLAGKERNLARDLAYGDRRRLEIAVA
                     LAAEPRLLFLDEPTAGMGAEGARQLAALIERLRQRVTIVIIEHDMPFLFSLADRISVI
                     QWGQVIAEGTPAELRADPWVARSNLGVIA"
     gene            3431..>4092
                     /locus_tag="VLN25_08225"
     CDS             3431..>4092
                     /locus_tag="VLN25_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSQ10587.1"
                     /translation="MLHVDSIDSSYGETQVLFKVSLEVAAGEVVALLGASGAGKTTTL
                     RSVLGLTPARSGRIEFDGADITREKSYRIARRGVGWVPEERRIVPSLTVRRNLEIAQK
                     STRFRAWTLRECCEIFTALGYLLDRESENLSGGEMQMVAISRALIGSPGLVLMDEPSQ
                     GLAPKVVQDVLRTVVRMKSEGLGILLVEQNVHAALSVADRVIVLDHGAVAYRGSAAEL
                     RD"
BASE COUNT          683 a         1332 c         1337 g          740 t
ORIGIN      
        1 caattgcgaa ctcttcctca gtgccgacga tcaggtcgca cgatgaaaga aactcgtgca
       61 gggtgtcggt gacaatggac gactcgacgt agcgtgtcgc cccatctcca agcggccgca
      121 gtccccacag caccggccgg aagtcgatgt cgagcgcgac acgcgtgccg gccgccaccg
      181 ctgccgcgat tgccttgcgg caggctgccc gtgtcgattc ctgcgacaag tgcgttccgg
      241 agatcagcag cgcgttcgcc gaggcgatca gactgtcgtc gacgtcatcg acggtgagcg
      301 ccatgtcggc gcaccggtcg cggtagaaca gcagcggaaa cgtgttctcg tcgcgaatac
      361 tgagaaagac gagggcggta aggtgtcgcg gatccgcaac cacgtgagtc acgtcgaccc
      421 cggcccgcgc cagctcattg cgaacgaact cgccgttctg ctcgtcaccg acgcgggtca
      481 gcatcgcggg acgcaggccg agccgcgcgc atccgacggc ggtattggcc ggcgacccgc
      541 cgaggtaacg agcaaaggtc gagacctcct gcagactcgc cccgatctgc tcaccataga
      601 ggtcgacggc cgcgcgtccg atgcagacga ggtcaaagcg gcgtgtcatc tgcgcgttcc
      661 gttgcggcgt gccggcgtct cggtcaggcg atggccgagc gcaatcacca gcgactgcgc
      721 aatcacgatc ggttcgacca gcgaccgaaa cggcaggcgg cggtcgtcgc ccagcagaat
      781 tgccagtgtc gaacacggga ccagcggcga agctcgactg tcggtgatgc ccagcacccc
      841 ggcgccacga ccgtgcgcct ggcgcgcaat gtcgatgacc tccggcgtgt agctgcgaaa
      901 gctgacgacc agcagcaagt cgcgcggcgc gagcgcagcg gcaagctgcg cgttcatccc
      961 gccaattccg tccaacagag tgacgcggcg ctccagctgc gccaatgcat aggcgagata
     1021 gctcacaacc gggaacgagc ggcgtgccgc caacaggtac acatgcccgc tcttcgcaag
     1081 caagtcgacc gccgcgtcca acttgcgctc gtcgagggtc ttcgtcagat gctgcagacc
     1141 gccgatcgcc tcgtcgacct gcatccttac gacgtcgcct gcggtcgcgg ccgccgtgct
     1201 ccgcctaagt ttctgaatcc gttcgcgata cgaaggtgcg tgcgcgacca gatgcgaacg
     1261 aaacacctgc cgtaacgccg cgaagccgtc aaaacccaga gccgccgcga agcgcaccag
     1321 cgcggacggc tgtacgccgg cggcctgcgc cagttccgcc gcggtgccaa aggcaaaggt
     1381 ctcgggagca tcgagcgcga gctgggcaat cgagcgcagt tggcgcggca gcgcttcgag
     1441 acggcccgcg atctccgcca tcaaaggctc gaatcccgca gtgcgctcgc gacgtgtgcc
     1501 gtgggtccga acggtcgccg actgcacctg ctctcctccc gaaacttttt gcttgtcgtc
     1561 cggcacaata gaacgtacat tttgttttgt ctagtgtatg gaatgaacat tccaccgttt
     1621 accgatcgcc cgatgccccc gcaacccgtc gtcgaaacat cgatgagtgc cgtgccgacc
     1681 ggtctgccac gcggacccgt gagcctccag gctgcacggc agggctttgc cggacccctt
     1741 gcgctcacgc tgctggtgct tgtaacgacg ccgctgtgga taaaggaaat cgggctctat
     1801 cagtacatcg cggtcgagat cgtgatctgg gcgttgttcg cgctgtcgta caacctgctg
     1861 ctcgggcagg ccggcctgcc gtcattcggc cacggcgctt tcttcggtgt cggcgcctac
     1921 gccttcggcc tggcggtcaa acacgtcgcg ccggacctct ggctcggact tggcgcggca
     1981 ctggccgccg ccgctctggc cggcgcgctg gtagcggcct tcatctcgca tcgacgcggc
     2041 atctactacg cgctgatgac gatcgccttc ggccaggtgc tgtggttcgt ggcgatcaaa
     2101 tggcacagcg tcacgggcgg cgaggacggt ctgctcggaa ttccacgacc gccggtcgat
     2161 ctcggcatca tcgagttgag cctgaacagc aacgaagctc tgttttactt cgcggtggtg
     2221 ctgttcaccc tggtcatcgc cttcctgtgg cagctgcagg acaccccctt tgggcgtgtg
     2281 atggcggcgg tcaagggcaa tgaggagcgc gcccgctact gtgggcatgc ggtgtggcgc
     2341 atcaagttca cgagcttcgt cttgtctgcg gcgctggctg gtctggccgg tgcgctgttc
     2401 gccatggcgc agaactccgc ctacccgaat gtgatgagcc tgcacaactc aggcttcgtc
     2461 gtgatgatgg tgctgatcgg cggcggcctg gtcagcttct ggggtccggt catcggcgcg
     2521 gccttcttca tcctggcgcg cgacctgctg ggtgcgacta ccgaggcctg gttgctgtgg
     2581 tacggattgc ttttcatggc ggtggtgctg ttcaagcccg aaggcatcgt cggcgcgtgg
     2641 cgcatgctgc ggcaaagggc gatgcgcgcc tcggcgggga gccactgatg gcgctgctcg
     2701 aggcccgcga actgcaccag cgctttgggc gccagatcgt gctcgacggc gtttcgctga
     2761 gcttcgagga aggctctctg tcaggaatca tcggtcccaa cggcgcgggc aagaccacct
     2821 gcttcaacgt gctgaccggc cgctacaagc ctgatcgcgg cagcgtgcac ttcgacggag
     2881 acgacatcac gggacgctca cccgccgcca tcgccaggcg aggcatcgcg cgctcgttcc
     2941 agttaatgaa cctgttcgac gcattggcgg ccatcgacaa cgtgatcgtc gcgatgcccc
     3001 ccttgcggcg gcgcgccttc cagccgttcg gcgccgtgcg tcgcgatatc gaattgcacg
     3061 cgcgagcgat ggcgctgctg gctgatgttg gccttgcggg caaggagcgc aatctcgcgc
     3121 gcgacctcgc ctacggcgat cgccgtcgcc ttgaaatcgc cgttgcactc gcggctgagc
     3181 cacggctgct cttcctcgac gagccgaccg ccggcatggg ggctgagggc gcgcgccagc
     3241 tggcggcact catcgagcgg ctgcgtcaac gcgtgaccat cgtcatcatt gaacatgaca
     3301 tgccgttcct gttctcgctg gcggaccgga tctcggtgat tcagtggggc caggttatcg
     3361 ccgaaggcac gccggccgag ctgcgcgccg acccatgggt cgcacgctcg aaccttgggg
     3421 tcatcgcctg atgctgcacg tggactccat cgacagctcc tatggtgaga cccaggtgct
     3481 gttcaaggta tcgctcgagg tggcagcggg tgaagtggtc gcactgctcg gcgccagcgg
     3541 cgccggcaag accacgactc tgcgatcagt gctcgggctg acgcccgcgc gctccggccg
     3601 catcgagttc gatggcgccg acattacgcg cgagaagtcc tatcgcatcg cccgtcgcgg
     3661 tgtcggctgg gtaccggaag aacgccggat agtgcccagt ctcaccgtcc gcaggaatct
     3721 cgagattgcg cagaagtcga cgcgcttccg agcgtggacg ctgcgtgagt gctgcgaaat
     3781 cttcactgcg ctcggctacc ttcttgaccg ggagtccgag aacctctcgg gcggcgagat
     3841 gcagatggtg gcgatctccc gtgcgcttat cggcagcccc ggactggtgt tgatggatga
     3901 gccgagtcag ggattggcac cgaaagttgt ccaggacgtc ctgcgcaccg tcgtgcgcat
     3961 gaagtccgaa ggactcggca tcctgctggt cgagcagaat gtgcacgcgg cgctttcggt
     4021 ggccgaccgc gtgatcgtgc ttgatcacgg cgccgtagcg tatcgcgggt ccgcggccga
     4081 actgcgcgac ga
//
LOCUS       DATIRT010000364         1786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_846110, whole genome shotgun sequence.
ACCESSION   DATIRT010000364 DATIRT010000000
VERSION     DATIRT010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846110"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VLN25_08230"
     CDS             <1..238
                     /locus_tag="VLN25_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10588.1"
                     /translation="PVMSQFGPRSNIELERLEAGNSRARSAQMLLEAGRTFRQFQSFC
                     QATQSLVRVDANNMEELFCIAELLAARGESRFSS"
     gene            242..1672
                     /locus_tag="VLN25_08235"
     CDS             242..1672
                     /locus_tag="VLN25_08235"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HSQ10589.1"
                     /translation="MIRAALNCGPSSRRMSQMAMGKRTLVEQPLFFTCNDLPRSEGHP
                     FYVMVNRVLAESEFDRYVESACQKFYAQVMGRPSVAPGIYFRMLLIGYFEGLDSERGI
                     AWRCSDSLSLRQFIGVALDQLTPDHSSVSRTRRLIDLETHQQVFRFVLHLLAQKKLLK
                     GKTMGVDSTTLEANAAMKSIVRRDTGVSYEQFLTKLAQASGIATPTREDLAKIDKDRK
                     NKGSNDDWTNPHDPDAKITKMKDGTTHLAHKAEHAVDMDSGAIVAVSLHDASKGDTNT
                     LLDTLVDATANLRPLVDDQSLEGKISDQWMSEVVTDKGYHSNQTLVDLETINIRSYLC
                     EPKRGRRKWADNPDARAAVYANRRRMKGRRGRRLRRKRGELLERPNAHCYETGRMRHL
                     YLRGRENILKRLLVHDAGCNLSLLMRKVFGHGTPRSLQGVARAFSATLGGLLMLLMTV
                     KRRWSERMRPRPCVFLMGRPQSIQFA"
BASE COUNT          375 a          519 c          572 g          320 t
ORIGIN      
        1 ccctgtcatg tcccagttcg gaccgcgctc gaatatcgag ctagagcgtc tggaggccgg
       61 caacagtcgg gctcgttccg cgcagatgct tctggaagcc ggaagaacat ttcggcaatt
      121 ccaatccttc tgtcaagcca cgcaatccct cgtgcgagtt gacgccaaca atatggagga
      181 gctcttctgc attgcagaac tcctagcagc ccgtggtgaa agtcgatttt cctcttgacc
      241 tttgatccgc gccgcgctga actgtggccc atcatcacgg aggatgtcgc agatggccat
      301 gggcaaacgc acgttggtcg aacagccgtt gttcttcacc tgcaacgatc tgccccgatc
      361 cgaggggcat ccgttttatg tcatggtgaa ccgggtgctg gccgagtcgg agtttgatcg
      421 gtacgtggag tcggcctgcc agaagtttta tgcccaggtg atgggccggc cctcggtggc
      481 gccggggatt tacttccgca tgctgctgat cgggtacttc gagggactgg acagcgagcg
      541 agggatcgcc tggcggtgct cggattcgct gtcgctgcgg cagttcatcg gcgtggcgtt
      601 ggaccagctc acgccggacc attccagcgt gtcgcggacg cgtcggctga tcgacctgga
      661 aacgcaccag caggtgttcc gcttcgtgct gcacctgctg gcccagaaga agcttctcaa
      721 gggcaagacg atgggggtgg actcgacgac gctggaggcc aacgcggcga tgaagagcat
      781 cgtgcgtcga gacacggggg taagctacga gcagttcctg accaagctgg cgcaggcgtc
      841 gggcatcgcc acgcccacac gggaagacct agccaaaatc gacaaagacc gcaaaaacaa
      901 aggctccaac gatgactgga ccaacccgca tgatccggac gccaagatca ccaagatgaa
      961 ggacggcacg acgcatctgg cgcacaaggc cgagcatgcc gtggacatgg acagcggcgc
     1021 gatcgtggcg gtaagcctgc acgatgccag caagggggat acgaacacgc tgctggacac
     1081 gctcgtcgac gcgacggcga accttcggcc actggtggat gaccagtcgc ttgaaggcaa
     1141 aatcagcgac cagtggatga gcgaggtggt gacggacaag gggtatcact ccaaccagac
     1201 gctggtggac ctggagacga tcaacatccg cagctacctg tgcgaaccga agcgtgggcg
     1261 gcgcaagtgg gcggacaatc ctgatgcccg ggctgccgtg tatgccaatc gtcggcggat
     1321 gaaaggcagg cgtggcaggc ggctgcgacg aaaacgtggc gagttgctgg agcgtcccaa
     1381 tgcccactgc tatgagaccg gacgcatgag acatctgtac ctgcgtggcc gagagaacat
     1441 tctcaaacgg ctgctggtgc acgatgccgg gtgcaacctg tcgctcctga tgcgcaaggt
     1501 gttcggccac ggcacgccgc gaagcctgca gggggtcgcg cgcgcatttt ccgcgactct
     1561 gggcggcctg ttgatgcttc tgatgacggt gaagagacga tggagcgagc gaatgcgacc
     1621 acgaccatgt gttttcctga tgggccgccc ccagtcgatt caatttgcct gagccccgat
     1681 cagcgacttt caccacgggc tgccaggggc tctgatccag cgcagtacac acgggctcgc
     1741 gagcacgcgc atttccaaaa caaagaaccc cgatcctctt gtggag
//
LOCUS       DATIRT010000365         4549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1042827, whole genome shotgun sequence.
ACCESSION   DATIRT010000365 DATIRT010000000
VERSION     DATIRT010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042827"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /gene="dprA"
                     /locus_tag="VLN25_08240"
     CDS             complement(<1..1065)
                     /gene="dprA"
                     /locus_tag="VLN25_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HSQ10590.1"
                     /translation="MQPAERAAWLRLIATPGIGPRTARHLLSSFGLPDAIFAAGMPAL
                     LRAVPEPLARLLASAPDGEIQSTIDATEHWLAASTSHALISLADPQYPALLLATPDPP
                     PLLFAVGRIELLSRPALAIVGSRNATKQGVANAEAFAGALARSGLTIISGLALGIDAA
                     AHRGALDADGDAGTIAVVGTGVDAIYPASNRALTHRVRAEGVVVSEFPLRTPAIAHNF
                     PRRNRIIAGLARGVLVVEAALRSGSLITARIAADAGRDVFAIPGSIHSPLAKGCHRLI
                     REGAKLVESAHDVAEDLGLERLPVALTGEVEPGPHAELLGLLGHDPIDLDTLAQRTGR
                     DAGTLAAKLLELELAQDVERL"
     gene            complement(1081..2304)
                     /locus_tag="VLN25_08245"
     CDS             complement(1081..2304)
                     /locus_tag="VLN25_08245"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HSQ10591.1"
                     /translation="MKKTSMGVLAAVACMGVTATGSALAQSATSRYPITSEQRGVADQ
                     VAQAGVPLSALAADAPERYVVKRGDTLWDLSRMYLTSPWRWPELWGMNKQQIANPHLI
                     YPGQELLLVKEDGRARLMIGGGESKEGKLQPTIRDMGSERAAIRSIPTNLIQPFLSQP
                     LVLESSTMNDAPRIIATQEGRVWVGRGDSAYARGITNDAVLNYSVFRPLRPLFDPDDV
                     NKKSPIAYEAVYLGTVNISRPGEVARVHVQESKQEIGVGDRMLPIEQQALISYIPKAP
                     SGPVEARVISIYDGVRYAGGGQIITLNRGSNEGLTIGDTLQLWRAGETIEDRTLPGRQ
                     FVKLPDEQIGLAFVFRAFPTISYALIQRGTQPVEVGDRASNPSDDTEIPRVRTEAETE
                     ADRVLRLPLRPMPPQ"
     gene            2642..3148
                     /gene="def"
                     /locus_tag="VLN25_08250"
     CDS             2642..3148
                     /gene="def"
                     /locus_tag="VLN25_08250"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HSQ10592.1"
                     /translation="MALLPILEYPDPRLCTVARPVTAFDDRLRKLVADMAETMYEAPG
                     VGLAASQVDVHERIVVIDTSETKDDLQVFINPEIVWASDEQVECEEGCLSVPGVYDAV
                     TRPARVRVKAFDERGAAFERDYTGLLAVCVQHEMDHLLGKVFVEYLSPLKQERIKTKM
                     KKRRTRAA"
     gene            3303..>4549
                     /locus_tag="VLN25_08255"
     CDS             3303..>4549
                     /locus_tag="VLN25_08255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HSQ10593.1"
                     /translation="MLDTATLRASWRQWWSPDWMFPCAGPRWLRLVWTFFFNCAIALA
                     LTLAAWAFARRVDLWQTLRWNFVIAQCIGFCIHGLFSLAKVVLGSDRIRGFGGTARVL
                     FYSALPIAGVLIGYALGLSIMGVDVPKLVVERPTLPLAIVLLSIVVSAMLYRHYGNKA
                     ALAEAQAEQARERARSTELERQAVTSKLKALQAQIEPHFLFNTLANVVSLIDTEPAQA
                     RRMLERLIELLRSSLAASRADRATLGQESALLEAYLDILKVRMGDRLDYTLDIPSELM
                     SANLPPLTLQPLVENAIRHGLEPKVDGGTVRVGAHARDGRLVLEVEDDGLGFEPKAGA
                     GVGLDNVRERLASQYGDGARLVIEEARPDTRVRLTLPLEAYLDILKVRMGDRLHYTLD
                     IPSELMSALLPPLTLQPLVENTIR"
BASE COUNT          757 a         1454 c         1521 g          817 t
ORIGIN      
        1 cagacgctca acatcttgtg ccagctccag ttcaagcagc tttgccgcaa gcgtccccgc
       61 gtcgcgaccg gtgcgctgcg ctagcgtgtc cagatcaatc gggtcatggc ccagaagtcc
      121 gagcagttcg gcgtgaggtc caggctcgac ctccccggtc aatgccacgg gcaagcgctc
      181 gagaccgagg tcttcggcga cgtcgtgcgc tgactcgacc agcttggcgc cttcgcgaat
      241 cagtcgatgg cagcccttgg caagcggcga gtggatcgag ccaggaatcg cgaacacatc
      301 acggcccgcg tcggcggcga tgcgggccgt gatgagcgag cccgagcgca gcgcggcttc
      361 aaccaccagt acgccgcgag caagccccgc gatgatgcga ttgcgacgcg ggaagttgtg
      421 cgcgatcgcc ggagtgcgca gcgggaactc ggaaaccacg acgccttccg cgcgaactcg
      481 atgcgtcaac gcgcgattgc tggccggata gatcgcgtcg actccggtac cgaccaccgc
      541 gatcgtgccc gcgtcaccgt ccgcatccag tgcgccccga tgtgcggcgg cgtcgatacc
      601 gagcgcgagc ccgctgatga tggtcagtcc ggaccgcgcc aacgctccgg cgaaggcttc
      661 tgcgttggcg acaccctgtt tggtggcatt tcggctgccg acgatggcca gcgccggccg
      721 gctcaacaac tcgatacgtc cgacggcaaa cagcagcggc ggcgggtccg gagttgcgag
      781 caacagcgct ggatactgcg gatccgcgag tgaaatgagc gcgtggctgg tcgatgcggc
      841 aagccagtgt tcggtggcat cgatcgtgga ctggatctcg ccgtcgggtg cactggcgag
      901 gaggcgcgca agcggctccg gcacggcgcg cagcaacgcc ggcatgccgg cggcaaagat
      961 ggcgtcaggc agtccgaacg aggaaaggag atgtcgggca gtgcgtggcc cgatacccgg
     1021 tgtagcaatc agccgcagcc aggccgcgcg ctccgccggc tgcattagcc gcgtcgcgat
     1081 ttactgcggc ggcattgggc gaagcggcag gcgcaagacg cggtccgctt cggtctcggc
     1141 ttccgtgcgc acgcgcggta tttccgtgtc gtcgctcggg ttggaggcgc gatcgccgac
     1201 ttcgacaggc tgcgtgccgc gctggatcag cgcatatgaa atcgtcggga acgcgcggaa
     1261 cacgaatgcg agtccgatct gctcatcggg aagcttgacg aactgccgac cgggcagggt
     1321 cctgtcttcg atcgtttcgc cggcgcgcca gagctgcagc gtgtcgccga tggtcaggcc
     1381 ttcgttcgag ccccggttca gcgtgatgat ctggccgcct cctgcatagc ggacgccgtc
     1441 gtagatcgaa atgactcggg cttcgaccgg gccggacggt gccttgggta tgtagctgat
     1501 cagcgcctgc tgctcgatcg gcagcatgcg atcgccgacg ccgatttcct gtttgctttc
     1561 ctgcacgtgg acgcgcgcca cttcgcccgg tctggaaatg ttcaccgtgc cgaggtatac
     1621 cgcctcgtag gcgatcggcg acttcttgtt gacgtcgtcc ggatcgaaca gcgggcgcag
     1681 cgggcggaac acgctgtagt tgaggaccgc atcgttcgtg atgccccgcg catacgcgct
     1741 gtcgccgcgg cctacccaga cccggccttc ctgcgtggca atgattcgtg gcgcgtcgtt
     1801 catcgttgac gactcgagca cgagcggctg cgacaggaac ggctggatga gattggtcgg
     1861 aatgctgcgg atcgccgcgc gttcggatcc catgtcacgg atcgtcggct gcaacttgcc
     1921 ttctttcgac tcgccgccgc cgatcatgag ccgcgcacga ccgtcttctt tcaccagcag
     1981 cagttcctga ccggggtaga tcaggtgcgg attggcaatc tgctgcttgt tcatgcccca
     2041 cagctccggc cagcgccacg ggctcgtgag gtacatgcgg gacagatccc agagcgtgtc
     2101 gccgcgtttg acgacgtagc gttccggcgc atcggcggcc agtgcagaaa gcggtacgcc
     2161 ggcctgggcg acctggtcgg cgacgccgcg ctgctccgag gtgatcggat agcggctggt
     2221 ggccgattgc gccagggcgg agcccgtcgc ggtcacgccc atgcaggcga cggcggcgag
     2281 tacgcccatg ctagtcttct tcatgcaagc tccctctggc cgtccggtgg ggttcgcacc
     2341 gtccctcagg ctcggcttcc cgacttgaaa tttgcggaac cgactgcgat ctaagctgag
     2401 acagggcgtc agattctgcc cctagtgcct tgatatagca acggatagcc aaaggggttg
     2461 atcccgactt ggcgcgatgc caaaaggcgt ggggcagcgt cggacgaggt cctgccgatc
     2521 actcatcaag tcgcagtgtg gcgggaaagc gggccggtcg ggcgcgtgaa aagcagggta
     2581 ggagggcggg acttagccgg atcatcctca gcacgacggg ccgggcattt ggagaagaca
     2641 cgtggcattg ctccctatac tcgaatatcc ggacccgcgg ctgtgcacgg tcgcgcggcc
     2701 ggtcaccgcg ttcgacgatc gactgcgcaa gctcgtggcc gacatggccg aaacgatgta
     2761 cgaggcgccg ggggttggcc tggcggcttc ccaggtcgac gtgcatgagc gcatcgtcgt
     2821 catcgacacg tcggagacca aggacgacct gcaggtcttc atcaatcccg agatcgtctg
     2881 ggcttccgac gagcaggtcg agtgcgagga gggctgcctg tccgtccccg gggtctacga
     2941 cgcggtaacg cgcccggcgc gggtccgcgt caaggcattc gacgaacggg gcgcggcgtt
     3001 cgagcgcgac tacaccggct tgctggcggt gtgcgtgcag cacgaaatgg atcacttgct
     3061 gggcaaggtc ttcgtcgagt acctgtcgcc gctcaagcag gagcgcatca agaccaagat
     3121 gaagaaacgc cggacgcggg ccgcctgagg gccgagcagc ccgccgcgtt tgccgtcgat
     3181 tggcgccact ccgccggcga ttcacgccag cgcagtggcg accgcgaccg tcgcgggcct
     3241 acagtcccgt tcgtttcgtg gaggctcctg ccgcccctgc tcgaactctg gacgcagatc
     3301 cgatgctaga cactgcaacg ctgcgcgcct cctggcgcca gtggtggtcg cccgactgga
     3361 tgtttccctg cgcgggtccg cgctggctgc ggctggtctg gacgtttttc ttcaattgcg
     3421 ccatcgccct tgcgctgacc ctggccgcgt gggcctttgc ccgccgcgtc gacctctggc
     3481 aaacgctgcg ctggaacttc gtcatcgccc agtgcatcgg cttttgcatc cacggcctgt
     3541 tcagccttgc gaaggtcgtg ctcggttccg atcgcatccg cggctttggc ggaaccgctc
     3601 gcgttctgtt ctattccgct cttccgatcg ctggcgtgct gatcggatat gcgttgggac
     3661 tttcgatcat gggagtcgat gttccgaaac tggtggtgga gcggccgacc cttcctctgg
     3721 ccatcgtcct gctctccatc gtcgtgtcgg ccatgttgta tcgccactat ggcaacaagg
     3781 ccgcgctggc cgaggcgcag gccgaacagg cacgtgaacg ggcacgttcc accgagctcg
     3841 agcggcaggc cgtcacgtcg aaactcaaag cgctacaggc gcagatcgaa ccgcactttc
     3901 tattcaacac gctggccaac gtggtgagcc tgatcgacac cgagccggca caggcgcgcc
     3961 gcatgctgga gcggctgatc gaactgctgc gttcgtcgct ggctgcgagc cgcgcggacc
     4021 gcgcgacgct ggggcaggag tctgcgttgc tggaggccta tctggacatc ctgaaggtca
     4081 ggatgggaga ccgcctcgat tacaccctcg atataccgtc cgagctgatg tcggcgaacc
     4141 tgccaccgtt gacgctgcaa ccgctggtcg agaacgccat ccgccacgga ctggaaccga
     4201 aagtcgacgg cggcacggtc cgggtcggtg cacatgcccg cgatggccgg ctggtcctcg
     4261 aagtcgagga cgatggactg ggtttcgagc ccaaggccgg cgccggggtc ggactggaca
     4321 atgtccgcga acgactcgcg tcgcagtatg gtgatggcgc ccgcctggtc atcgaggaag
     4381 cccggccgga cacgcgggtc cgactcaccc tgccgctgga ggcctatctg gacatcctaa
     4441 aggtcaggat gggagaccgc ctccattaca ccctcgatat accgtccgag ctgatgtcgg
     4501 cgctcctgcc accgttgacg ctgcaaccgc tggtcgagaa caccatccg
//
LOCUS       DATIRT010000366         9150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1239908, whole genome shotgun sequence.
ACCESSION   DATIRT010000366 DATIRT010000000
VERSION     DATIRT010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9150
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239908"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..1214
                     /locus_tag="VLN25_08260"
     CDS             219..1214
                     /locus_tag="VLN25_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HSQ10594.1"
                     /translation="MDRLTGMQVFTEVADRASLTEAARTLDMSRAMVSRYLESFERWL
                     GVRLLHRTTRRVSLTEAGEEALERCRPILELSRDVQTVAGARRAAPVGKLRITASTSF
                     AETFLTDVVTDFLSRYPQTQVELIALERTINLVEERIDLAVRIGNVLDDTLVARKLGV
                     CRSVVCAAPAYIRASGLPRTPDDLQKHRCITHAYVGRTEFRLHRMGRALRIPVKGPFQ
                     SNEAAVTRRAALAGAGIAMLPTYFVSADLASGALIRLLPDCEPEPLGIYAAYLSRLHQ
                     PQLLRLMIDFLADRLSGDVAPWDRVISSPPQAASSIAKRRVSSQPTARRRHVARG"
     gene            complement(1296..2525)
                     /gene="clsB"
                     /locus_tag="VLN25_08265"
     CDS             complement(1296..2525)
                     /gene="clsB"
                     /locus_tag="VLN25_08265"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HSQ10595.1"
                     /translation="MMERVRPALRRPRFEITTRPLVYGGNRVRLLQNGDEFFPNLLAA
                     INSAARSIYLETYIFAQDNIGLRVSDALAAAADRGVAVRLVVDGFGTGAFAPELKARL
                     ENVGAQVIIFRRTRWWRLERRLLRRMHRKLMLVDDQLAFVGGINIIDDHHHPDPEPAR
                     LGPRFDFAVLCEGPIVASVALAMKRLWWTLQLPQSRGRSGPAPALALESIPPPFVDGV
                     RAALLLRDNLRYRRTIESAYMEVIKSAHKEVLIACAYFLPGRTFRKALVDCVRRGVRV
                     RLLVQGRIEYAIQHYGQEALYSQFLEAGVEIHEYETSYLHAKVAVVDESWATVGSSNI
                     DPYSLLLAREANVAVYDTAFARELRTALERAIDEHSRTVQPQEFARRGLIRRMVNWIA
                     YGIVRFGAVVLAGGRDY"
     gene            complement(2522..3331)
                     /locus_tag="VLN25_08270"
     CDS             complement(2522..3331)
                     /locus_tag="VLN25_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003269634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HSQ10596.1"
                     /translation="MQLRVATYNIHKGVMGLRKRSRIHDLRVALHAIDADIVFLQEVQ
                     ERNERLARRPDHPNGTQLDVLCTGGYLHRAYGMNAVYPHGHHGNAIVSRHTIGQYLNH
                     DISDHALEKRGMLHAISHFESRRGAVDIHLINTHFGLIKRSRVRQAEFLIDFVRSEVP
                     ADAPLIIAGDFNDWQQGVDEVLHRELDVVEAAGDYNRQQSRRRRLDRLMPWRNGARPL
                     VARTYPSVMPWLQLDRIYVRGFKVRDVSVPKGIEWSQRSDHMPLIANLEIA"
     gene            complement(3344..4867)
                     /locus_tag="VLN25_08275"
     CDS             complement(3344..4867)
                     /locus_tag="VLN25_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10597.1"
                     /translation="MPPPSVNWRNALAASLAATAALGGCASLPDPYAVPPASEQFARA
                     DALGDCMRRLREVDARIDAAGVRDAQAARVEGYPFLRVDRFTASRSPGDRDAPVVAQA
                     KLDRMAALDAEAREYESANARIADVDRAALQRCRDELVAATRVDAERVSNLANAPHSY
                     NDAMRVVGLYPLTKFPFTWGVAAWQQTTRDLYATPFPELTVNGKRIRYVPGPGGSEHL
                     AGLLPTDSAGAGPLRMPVVTPARAWQLLQQHAPVLVVDTVDDDDRIGRLAWRKSGDDL
                     YVAVDVSEPATYVRAAWTEIGGKPTLQLVYTFWFPARPASHPLDWVAGRLDALVWRVT
                     LDARGRPLVYDSIHACGCFHMFFPTERVRERPGPLEHEGIFDETMFSPQVVRSPGPGE
                     RVIVYLGSGDHNIQRVAIDTPKPAPGVPYRLKDENELRALPLPAVAGGGTRSIYGPDA
                     LVPGSQRPERFLFWPMGVPSAGQMRQWGHHATAFVGRRHFDDPLLIDRYFTLAPGAD"
     gene            complement(4824..5276)
                     /gene="nudB"
                     /locus_tag="VLN25_08280"
     CDS             complement(4824..5276)
                     /gene="nudB"
                     /locus_tag="VLN25_08280"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HSQ10598.1"
                     /translation="MTPVVKIPESVLVVIHTLALDVLLLERADHPGFWQSVTGSLDYP
                     GEDLRCACAREVAEETGLAAAPERFEDWTLEHRYEIYSEWRHRYAPGVTHNTEHVFGL
                     RVVERYSPQLAPREHRSCRWLPWRDAADACFSWTNAAAVRELAQRTRG"
     gene            complement(5353..7203)
                     /gene="aspS"
                     /locus_tag="VLN25_08285"
     CDS             complement(5353..7203)
                     /gene="aspS"
                     /locus_tag="VLN25_08285"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HSQ10599.1"
                     /translation="MRTEYAGRVDEKFIGQTVTLFGWVHRRRDHGGVIFIDLRDREGL
                     VQVVCDPDRAEVFANAERVRNEFCLKITGQVRKRPEGTTNETLVSGGIEVLCTQLEIL
                     NASVPVPFQLDDENLSETTRLTHRVLDLRRAPMQKNMMLRYKVAMAVRRYLDAAGFVD
                     IETPMLTKSTPEGARDYLVPSRVHAGEFFALPQSPQLFKQLLMVAGFDRYYQIVKCFR
                     DEDLRADRQPEFTQIDIETSFLDESEIRAIMEEMIRTVFAEVMNVTLPKPFPVMTYGD
                     AMRLYGSDKPDLRVVLQLTDVSDAMRDVEFKVFKAATEMKDGKVVALRIPRGGEMPRS
                     EIDGYTEFVKIYGAKGLAYIKVNEKAKGRDGLQSPIVKNLNDAALAAVLELTGAADGD
                     LIFFGADRAKIVYDSLGALRTKIGHSEFGRAHGLSAAGWKPLWVIDFPMFEFDEDEQR
                     YVAAHHPFTSPKEGHEDFLQTDPSKALAKAYDMALNGWEIGGGSVRIHREEVQSKVFR
                     ALKIGVEEAREKFGFLLDALQYGAPPHGGIAFGLDRIVTMMAGAESIRDVIAFPKTQR
                     AQCLLTKAPSPVDEKQLRDLHIRLRGEVRAESESARPNTPQRHAGELNQK"
     gene            complement(7534..8175)
                     /locus_tag="VLN25_08290"
     CDS             complement(7534..8175)
                     /locus_tag="VLN25_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HSQ10600.1"
                     /translation="MFRKYLITGLLIWLPLGITLLILETIIGWMDQSLLLLPPAWRPD
                     ALLGFRIPGLGALLAVAVVLLTGVLARNIVGRRLLLWWEGLLGRIPIVRSIYSSVKQV
                     SDTMLSPKGNAFRKVVLVEFPQPEQWTLAFIVGEPAGAVAEQLGEAWVTVYVPTAPNP
                     TSGYVLMMPPSRVRDVDVSVDDALKFHVSLGVVEPGARPVRPAVLTSASPPPA"
     gene            complement(8280..8645)
                     /locus_tag="VLN25_08295"
     CDS             complement(8280..8645)
                     /locus_tag="VLN25_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HSQ10601.1"
                     /translation="MPIYAYQCAECRHEVEVLQKISDAPLTDCPSCHRSSLVKKVTAA
                     GFQLKGSGWYATDFKGNGTKAKDSKAAEGPAKDGEAKSDAAKPAETPAAGSKTAESAS
                     PTPAPAAGGSTGGPPPAAS"
     gene            8848..>9150
                     /locus_tag="VLN25_08300"
     CDS             8848..>9150
                     /locus_tag="VLN25_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HSQ10602.1"
                     /translation="MATRKKAPAPTSDARAALKGIDAFTADKVKVAATDIDGVLRGKY
                     LHKDKFASAVDCGFGFCDVVFGWDMMDSVYDNTTVTGWQHGFPDALVRLDLSTGRRV"
BASE COUNT         1643 a         2997 c         2959 g         1551 t
ORIGIN      
        1 gtcggcgttg tagacctgaa gggacagcgc ggcgggactc gcctgggcgc cgacctcgcc
       61 tgcgacgaca ccgagtgtca ggctggcaaa tagcggaacg agaggtttca tcattgaatg
      121 gctcgcagtg gggtgagtga cggcatcact gtatttgcaa ccaaatcatg gataaacagt
      181 agactctcca acactttgtc taccagatcg agctaatcat ggatcgcctc accggcatgc
      241 aggtgttcac cgaggtcgcc gatcgcgcca gcctgaccga ggcggccaga acactcgaca
      301 tgtcgcgggc catggtgtcg cgctatctgg aaagcttcga gcgttggctc ggagtccgac
      361 tgctacatcg caccacgcga cgagtgagtc tcaccgaggc cggcgaagag gcgctagagc
      421 gctgccgccc gatcctcgaa ctctcgcgcg acgtgcagac cgtggccggt gcgaggcggg
      481 cggccccggt gggcaaactg cgcatcaccg ccagcacgtc gtttgccgaa actttcctga
      541 ccgacgtagt gacggacttc ctttcccgct acccgcaaac gcaggtcgaa ctgatcgcac
      601 ttgagcgaac catcaatctc gtggaggagc gcatcgacct ggcggtgcgg atcgggaacg
      661 tccttgacga cacgctcgtc gcgcgcaagc tgggcgtgtg tcgatccgtc gtttgtgcgg
      721 cgcccgcgta catcagggct agcggccttc cccgaacgcc ggacgaccta caaaagcacc
      781 gatgcatcac ccacgcctac gtgggccgta ccgagttccg cctgcatcga atgggccggg
      841 cactgcggat acccgtgaaa ggaccctttc agagcaacga agccgctgtc acgcgccgag
      901 ccgcgctcgc cggggccggg atcgcgatgc tgccgaccta ttttgtcagt gcagacctcg
      961 cctccggcgc gctcattcgc ctgctgccgg actgcgaacc cgagccgctt ggtatttacg
     1021 cggcgtactt gtctcgcctg catcagccac agttgctgcg attgatgatc gactttctgg
     1081 cagatcgcct gagcggagac gtggcgccct gggaccgtgt gatctcctcg ccaccgcaag
     1141 ctgcaagttc aattgccaag cgccgggtca gcagccaacc aacggctcgc cggagacacg
     1201 tagcgcgcgg ctagtcggtc gggaacggcc gcttgcggcg ttaacctagg accaccagcg
     1261 gtcgaactcc gtccaatccc actcgcggag actcactagt aatcccgccc ccctgccagc
     1321 acgactgcgc cgaagcggac gatgccgtaa gcgatccagt tgaccatccg ccgtatcaga
     1381 ccgcggcgtg cgaactcctg aggctggacg gtgcgcgagt gttcgtcgat cgcgcgttcc
     1441 agcgccgtcc gcagctcgcg cgcaaaggca gtgtcgtaga cggccacgtt ggcttcgcgc
     1501 gccagcagaa gactgtaggg atcgatgttg ctggatccca ccgtggccca ggactcgtcc
     1561 accacagcga ccttggcatg caggtaactc gtctcgtact cgtggatctc gacgccggct
     1621 tcgaggaact gtgaataaag cgcctcctga ccgtagtgct ggatcgcata ctcgatccgt
     1681 ccctgcacaa gcagtcgaac ccgcacgccg cgccgcacgc agtcgaccag cgccttgcga
     1741 aacgtccggc cgggcaggaa gtaggcgcac gcgatcagca cttccttgtg cgcagacttg
     1801 atcacctcca tgtaggcaga ttcgatcgtc cgccggtagc gcaggttgtc tcgcagcagc
     1861 agcgccgcac gtaccccatc gacgaagggc ggtggtatcg actccagcgc cagcgccggc
     1921 gcaggaccgg agcgaccacg actctgcggc agctgcaggg tccaccacag ccgtttcatg
     1981 gccagggcga ccgacgcaac gatcgggcct tcgcagagca ccgcgaagtc gaagcgcggg
     2041 cccagccggg ccggctcggg atccggatga tggtgatcgt cgatgatgtt gatgccgccc
     2101 acaaaggcga gctggtcgtc gaccagcatg agcttgcgat gcatgcggcg caggagtcgc
     2161 cgctccaggc gccaccagcg cgtgcggcgg aagatgatga cctgcgcacc gacgttttcg
     2221 agacgggcct tcagctcggg ggcaaaggcg ccggttccaa atccgtcgac gaccagacgc
     2281 acggcgacgc cacgatcggc cgccgccgcc agtgcgtcgc tgactcgcag gccgatgttg
     2341 tcctgggcaa agatgtaggt ctcgagatag atggagcgtg cggcgctgtt gatcgccgcc
     2401 agaagattgg gaaagaattc gtcgccgttt tgaagcagac ggacgcggtt gccgccgtag
     2461 accagcggac gcgtcgttat ctcgaagcgc ggccgccgga gtgccggccg cacgcgctcc
     2521 atcatgcgat ctcgaggttg gcgatcagag gcatgtggtc cgaacgctgc gaccactcga
     2581 tgcccttggg cacgctgaca tcgcgaacct tgaaaccgcg cacatagatg cggtccagct
     2641 gcagccacgg catgacgctg ggataagttc gcgccaccag cggtcgcgcg ccgtttcgcc
     2701 acggcatcag ccggtccagt cggcgacggc gactttgctg gcggttgtag tcgccggccg
     2761 cctcgacgac gtccagttcg cgatggagga cttcgtcgac gccctgctgc cagtcgttga
     2821 agtcgccggc gatgatcagc ggcgcgtcgg ccggaacctc gctgcggacg aagtcgatca
     2881 ggaactcggc ctggcgcaca cggctgcgct tgatcaggcc gaagtgcgta ttgatgagat
     2941 ggatgtcgac cgcaccacgc cgcgactcga agtgcgagat cgcgtgcagc attccgcgct
     3001 tttccagggc gtggtccgaa atgtcgtgat tcaggtactg gccgatcgtg tggcgcgaca
     3061 caatcgcatt gccgtggtgg ccgtgcggat agaccgcgtt catgccgtag gcgcgatgca
     3121 gatagccgcc cgtgcacagc acgtcgagct gggtaccgtt cggatggtca ggccggcgcg
     3181 ccagacgttc gttgcgttcc tgcacttcct gcagaaagac gatgtcggcg tcgatcgcgt
     3241 gcagcgcgac gcgcaggtcg tggatgcgcg agcgcttgcg cagtcccatt acgcccttgt
     3301 ggatgttgta cgtcgcgacg cgaagttgca tgaaaccagt tactcagtcc gcaccgggcg
     3361 caagcgtgaa atagcgatcg atcagcagcg gatcatcgaa atgacgacgg cccacgaacg
     3421 ctgttgcgtg atgaccccat tggcgcatct ggcccgccga cggcacaccc atcggccaga
     3481 agaggaaacg ctccggccgc tgcgagccgg gcaccagcgc atcgggcccg taaatgctgc
     3541 gcgtgccccc gccggccacc gccggcagcg ggagcgctct cagttcgttt tcgtccttca
     3601 gccgatacgg cactccgggt gccggcttcg gcgtatcgat cgccacccgc tggatgttgt
     3661 gatcgcccga acccaggtag acgatgacac gctcgcccgg gcccggcgaa cgcacgacct
     3721 ggggtgaaaa catagtctcg tcaaaaatgc cctcgtgctc gagcggaccg gggcgctcgc
     3781 gcacgcgctc ggtcggaaag aacatgtgaa agcagccgca cgcatggatc gagtcataga
     3841 ccagcgggcg gccccgggca tcgagcgtga ctcgccagac gagcgcgtcg agccgacccg
     3901 ctacccaatc gagtggatgg ctggcaggcc gggccggaaa ccagaatgtg tagacaagct
     3961 gcagcgtggg tttgccgccg atttcggtcc aggcggcgcg tacgtacgtg gccggctcgc
     4021 tgacgtcgac agcaacataa agatcgtcgc cacttttccg ccatgccagc ctgccgatgc
     4081 ggtcatcgtc gtcgacggtg tcaacgacca gcaccggagc atgttgttgc agcagttgcc
     4141 aggcgcgcgc aggcgtcacc accggcatac gcagcgggcc cgcgccggcg ctgtcggtcg
     4201 ggagcaaacc ggcgagatgc tccgaaccgc ccggcccggg cacgtagcgg atgcgcttgc
     4261 cattcaccgt cagttcggga aacggcgtgg cgtacaggtc gcgcgtggtc tgctgccagg
     4321 cggccacccc ccaggtgaag ggaaacttgg tcagcgggta cagaccgacg acccgcatcg
     4381 catcgttgta actgtgcgga gcattggcaa ggttcgacac ccgttcggca tcgacacggg
     4441 tggcggccac gagctcgtca cggcaacgct ggagcgcggc ccggtcaacg tccgcaatgc
     4501 gcgcattggc gctttcgtac tctcgcgctt cggcgtcgag cgcggccatt cgatcgagtt
     4561 tcgcctgcgc caccactggg gcgtcgcggt cgcccggtga ccgcgaggcg gtgaaccgat
     4621 cgacccgaag gaacggatag ccctcgacgc gcgcggcctg cgcgtcgcgc acaccggcgg
     4681 cgtcgattcg cgcatccact tcgcgcaggc ggcgcatgca gtcgcccagc gcatcggcac
     4741 gagcgaactg ctcgctcgcg gggggcacgg catagggatc cggcagagat gcacagccgc
     4801 ccagtgccgc cgttgcggcg agactagccg cgagtgcgtt gcgccagttc acggacggcg
     4861 gcggcattgg tccaggaaaa gcaggcatcc gcggcgtcgc gccacggcag ccatcgacag
     4921 gaacgatgct cgcgcggtgc aagttgcgga ctgtagcgtt caaccacacg gaggccgaag
     4981 acatgctccg tattatgcgt aacgccgggc gcataacgat gacgccactc ggaatagatt
     5041 tcgtagcggt gctcaagggt ccagtcctcg aaccgttctg gcgcggcggc gagtccggtt
     5101 tcctcagcga cctcgcgggc gcaggcgcac ctaagatctt cgccgggata gtcaaggctg
     5161 ccggtcacgg actgccagaa cccgggatgg tcggcacgct cgagcaagag cacgtcgagt
     5221 gccagcgtat ggatcacgac cagcaccgac tcgggaatct tgaccaccgg cgtcacgaaa
     5281 ccggcgctcc gacccggcga ccggcgacgc tcatcgcaca cgcaacggcg tccaccgtcc
     5341 cggggccccg tcctacttct gattcagctc gcccgcatgg cgctgcggcg tgttgggccg
     5401 cgccgactcg gactccgcac gcacttcgcc acgcaggcgg atgtggagat cgcgcaactg
     5461 tttctcgtcg acgggtgacg gcgccttcgt cagcaggcac tgtgcccgtt gcgtcttggg
     5521 gaacgcgatc acgtcgcgga tcgactcggc gccggccatc atcgtgacga tgcgatcgag
     5581 cccgaacgcg atcccgccgt gcggcggcgc gccgtactgc agcgcatcca gcaggaagcc
     5641 gaacttctcg cgtgcttcct cgacgccgat cttcagcgcg cggaagacct tgctctgtac
     5701 ttcttcgcga tggatccgca ccgagccgcc gccaatttcc catccattca acgccatgtc
     5761 gtaggccttg gccagcgcct tggaaggatc cgtctgcagg aagtcctcgt gaccttcctt
     5821 cgggctggtg aacgggtgat gtgcggcgac gtagcgctgc tcgtcctcgt cgaactcgaa
     5881 catcgggaag tcgatcaccc acagcggctt ccagcccgcg gccgacaggc catgcgcgcg
     5941 cccgaattcg ctgtggccga tcttcgtgcg caaggcaccc agcgagtcgt aaacgatctt
     6001 ggcgcgatcg gcgccaaaga agatcaggtc accgtcggcc gctccggtca gttctagcac
     6061 cgcagcgagc gccgcgtcgt tcaggttctt gacgatcggg ctctgcaggc cgtcgcgacc
     6121 cttggccttc tcgttgacct tgatgtaggc aaggcccttg gcgccgtaga tcttgacgaa
     6181 ctcggtgtag ccatcgattt ccgaacgcgg catctcgccg ccgcgcggga tgcgtagcgc
     6241 aaccaccttg ccgtccttca tctcggtggc ggccttgaag accttgaact cgacgtcccg
     6301 catggcgtcg gacacgtcgg tcagttgcag aacgacccgc aggtccggct tatcggagcc
     6361 gtaaagccgc atcgcgtcgc cataggtcat gacggggaac ggcttcggca gcgtgacgtt
     6421 catcacctcg gcaaagacag tccggatcat ctcttccatg atcgcgcgga tctcggactc
     6481 gtcaaggaac gaggtctcga tgtcgatctg cgtgaattcg ggctgtcggt cggccctgag
     6541 gtcctcgtcg cggaagcact tgacgatctg gtagtagcgg tcgaagcccg cgaccatcag
     6601 cagctgtttg aagagctgcg gcgattgcgg cagcgcgaag aactcgccag cgtgcacgcg
     6661 tgacggcacc aggtagtcgc gcgcgccttc aggcgtgctc ttggtcagca tcggcgtctc
     6721 gatgtcgaca aagcccgcgg cgtcgagata acgccggacg gccatcgcca ccttgtagcg
     6781 cagcatcatg ttcttctgca tcggcgcgcg ccgcaggtcg agcacgcgat gcgtcagccg
     6841 tgtcgtctcc gagaggttct cgtcgtcgag ctggaacggc accggcaccg aagcgttgag
     6901 gatctcgagc tgcgtgcaaa gcacttcaat tccgccggac accagcgtct cgttggtcgt
     6961 gccttcgggg cgcttgcgca cctggccggt gatcttcagg cagaactcgt tgcggaccct
     7021 ctcggcgttg gcgaacacct ccgcacggtc cgggtcgcag acgacctgca cgaggccctc
     7081 gcgatcccgc aggtcgatga aaatgacgcc gccgtgatca cggcgacgat gcacccaacc
     7141 gaacagggtg acggtctgac cgatgaactt ctcgtccacc cggccggcat attcggtacg
     7201 catgaaatgc tccgctgtga ctggcagtcc gcggctcgac gatccggcgc gactgcgcaa
     7261 aaccagtgat tatccccgag gggctcgtct tcagcttggt ggccggaccg acgccatgac
     7321 gacgcgcgac ggatccggca gcagtggggc cgaacagcgc taccggcccg accctatcgg
     7381 tttcgctgat tggacgacgc ggaccgcatc accggacgag cacacccgcc agcgcgtttg
     7441 tcggcgccgt ggccctgcat ccggtgtttc gtcgtcatga gccgaacctt agcaggggac
     7501 tgcacgggac tcaaagtctg gggctcgagc gcctcaggcc ggaggcggcg acgcgctcgt
     7561 cagcacggcg ggccgcaccg gacgcgcacc gggttccacc acaccgagcg agacatggaa
     7621 tttcagcgcg tcgtcgacgc tgacatccac gtcccgcacg cgcgacggcg gcatcatcag
     7681 cacatatccg gaggtcgggt tgggcgcggt cggaacatag acggtgaccc aggcttctcc
     7741 cagctgctct gcgaccgcgc ccgccggctc gccgacgatg aacgccagcg tccattgctc
     7801 aggctgcggg aactcgacca gcacgacctt gcggaacgcg ttgcccttgg gcgagagcat
     7861 cgtgtccgac acctgcttca cgctcgagta gatcgagcgc acgatcggga tccggcccag
     7921 cagcccttcc caccagagca gcagccggcg cccgacgatg ttgcgcgcga gtacgcccgt
     7981 gagcagcacg acggccaccg ccagcaacgc cccgaggccg ggaattcgga agccgagcag
     8041 tgcgtccggc cgccacgccg gcggcagcag cagcagcgac tgatccatcc agccgatgat
     8101 cgtctcgagg atcagcagag tgatcccaag gggcagccag atcaggaggc cggtgatcag
     8161 gtacttgcga aacatgtgag gtcccaactg cgccgccgcg gccggcgcgt ggccctgaga
     8221 aagaaaaagc cgaggcaccg ccgcggctcc gttgctttcg agaatagccg atcagtccat
     8281 cacgacgcgg cgggaggggg cccaccggtg ctgccgccgg cggcgggagc cggtgtgggc
     8341 gatgcacttt cggcagtctt ggagccggcg gccggagttt cggcgggctt ggccgcgtcg
     8401 gacttcgcct ctccgtcctt cgctggaccc tcggcagcct tgctgtcctt ggccttggtt
     8461 ccgttgccct tgaagtcggt cgcgtaccag ccgctgccct tcagttgaaa gcccgccgcg
     8521 gtgaccttct tcacgaggct gctgcgatgg caggacggac agtcggtcag cggcgcgtcg
     8581 gagatcttct gcagcacctc gacctcgtgg cggcactcgg cgcactgata ggcataaatc
     8641 ggcatgggct aaaactcgct tcgcggtctc gtgtctctaa tggcttgcta actgcttgct
     8701 gattgatttg attgaattgt aagggagcag ctccgcggct cgcgaccgga gataaaggcg
     8761 taatccgggc gtatcaagcg gccgagcgcg ccccttggac tttgcccgcc gctgttgcac
     8821 actgccccgc cccaggagga gacacgcatg gccacaagaa agaaggcgcc cgcgccgacg
     8881 tcggatgcac gagccgccct gaagggcatc gatgcgttca cggccgacaa ggtcaaggtg
     8941 gctgccaccg acatcgacgg agtgctgcgt ggcaaatacc tgcacaagga caagtttgcg
     9001 tccgcggtcg actgcggctt cggcttctgc gatgtcgtgt tcggctggga catgatggac
     9061 agcgtctacg acaacacgac cgtcaccggc tggcagcacg gctttccgga tgcgctggtg
     9121 cgactcgatc tctcgacggg tcggcgcgtg
//
LOCUS       DATIRT010000367         1539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1338006, whole genome shotgun sequence.
ACCESSION   DATIRT010000367 DATIRT010000000
VERSION     DATIRT010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338006"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="VLN25_08305"
     CDS             <1..713
                     /locus_tag="VLN25_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HSQ10603.1"
                     /translation="GGRPFVDKLKHVAKDAKAIISWGSCASWGCVQAAKPNPTQATPV
                     HKVIFDKPIIKVPGCPPIAEVMTGVVTYVLTFDRLPEMDAQGRPKMFYSQRVHDKCYR
                     RPHFDAGQFVEKFDDAGARMGYCLYKVGCKGPVTYNACSAIRWNEGLSFPIQSGHGCI
                     GCSEDDFWDKGSFYDHLTTIRPPGVAGLGIEGTATTFGVAAVAVAGAALAAHAAVSAI
                     KQARSHSRNDKVNETVEK"
     gene            716..>1539
                     /locus_tag="VLN25_08310"
     CDS             716..>1539
                     /locus_tag="VLN25_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HSQ10604.1"
                     /translation="MPTIQTQGFTLDNSGRRIVVDPITRIEGHLRIEVNVDKDNVIRN
                     AVSTGTMWRGLEVILKGRDPRDAWAFVERICGVCTGVHALASVRSVEDALGIKIPKNA
                     NIIRNLMHATLYAQDHLVHFYHLHALDWVDVVSALKADPKKTSEIQQSISPWPNSSPG
                     YFREVQSRLKKFVESGQLGIFANAYWGNPAYQLPPEVNLLATTHYLEALDFQKEIVKI
                     QTIFGGKNPHPNWLVGGVPSPINVDGTGSIGAINMATLNMVKDIVDRTIEFVDQVY"
BASE COUNT          312 a          502 c          473 g          252 t
ORIGIN      
        1 ccggcggccg cccgtttgtc gataagctca agcacgtcgc caaggacgcc aaggccatca
       61 tctcgtgggg ctcgtgcgca tcgtggggct gcgtgcaggc cgcgaagccg aatccgacac
      121 aggccacacc ggtccacaag gtcatcttcg acaagccgat catcaaggtg cccggctgcc
      181 cgccgatcgc cgaagtgatg acgggtgtcg tgacctatgt gctgaccttc gaccgactgc
      241 ccgagatgga cgcccagggc cgaccgaaga tgttctacag ccagcgcgtg cacgacaagt
      301 gctatcgccg gccgcacttc gacgccggcc agttcgtcga gaagttcgac gatgccggcg
      361 cgcgcatggg gtactgcctc tacaaggtgg gctgcaaggg tcccgtcacc tacaacgcct
      421 gctccgcaat ccgctggaat gaaggactga gcttcccgat ccagtcgggc cacggctgca
      481 tcggctgttc cgaagacgac ttctgggaca agggctcgtt ctatgaccac ctgacgacga
      541 tccggccgcc gggagtcgcg ggactcggca tcgagggcac ggcgaccaca ttcggcgtcg
      601 cggcggtggc cgtcgcaggc gcagcgcttg cggcacacgc ggccgtcagt gcgatcaagc
      661 aggcgcgtag ccactcgcgc aatgacaaag tgaacgagac ggtggagaag taatcatgcc
      721 gaccatccag acacaaggtt tcacgctcga caacagcggc cggcgcatcg tcgtcgatcc
      781 gatcacccgg atcgagggtc acctgcgcat cgaggtcaat gtcgacaagg acaacgtgat
      841 ccgcaacgcg gtctcgaccg gaacgatgtg gcgtggcctc gaggtcatcc tcaagggtcg
      901 cgacccgagg gacgcctggg ccttcgtcga acggatctgc ggtgtgtgca ccggcgtgca
      961 cgcgctcgca tcggtgcgct cggtcgagga cgccctcggg atcaagatcc cgaagaacgc
     1021 aaacatcatc cgcaacctga tgcatgcgac gctgtacgcg caggaccatc ttgtccactt
     1081 ctatcacctg catgcgctcg actgggttga cgtggtctcg gcgctgaagg cggacccgaa
     1141 gaagacttcc gagatccagc agtcgatctc gccgtggccc aacagctcgc cgggctactt
     1201 ccgcgaagtg cagagccgcc tgaagaagtt cgtcgagagc ggccagctcg gcatctttgc
     1261 caacgcctac tggggaaacc cggcctacca gctgccgcct gaagtcaacc tgctggcaac
     1321 gacgcactac ctcgaggcgc tcgacttcca gaaggagatc gtcaagatcc agaccatctt
     1381 cggcggcaag aatccgcacc cgaactggct cgtcggcggc gtgccgtcac cgatcaatgt
     1441 cgacggcacc ggttcgatcg gcgcgatcaa catggccacg ctcaacatgg tcaaggacat
     1501 cgtcgaccgc acgatcgagt tcgtcgacca ggtctatat
//
LOCUS       DATIRT010000368         3957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1043734, whole genome shotgun sequence.
ACCESSION   DATIRT010000368 DATIRT010000000
VERSION     DATIRT010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043734"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /gene="ugpC"
                     /locus_tag="VLN25_08315"
     CDS             <1..927
                     /gene="ugpC"
                     /locus_tag="VLN25_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HSQ10605.1"
                     /translation="RMVAGLEEISGGTIRIGERIVNDLEPKDRDCAMVFQNYALYPHM
                     SVYDNMAYGLKIRKLPKPDIERRVHQAAGILELEELLQRKPRQLSGGQRQRVAMGRAI
                     VREPAVFLFDEPLSNLDAKLRVQTRLEIQKLHRRLGTTSLYVTHDQVEAMTLAQRMIV
                     MNAGRAEQVGTPDDVYSRPATTFVASFIGSPPMNLFEGRVVADGHSFETKDGVRLALP
                     AAVSALAGRVLVMGVRPEHLNLGGEGLTMVVETVERLGAEHLIHGVVAGHDVIVRTGP
                     DDSPAPGSNLSLGFPASAIHWFDASTRQRIAV"
     gene            complement(936..1391)
                     /gene="fur"
                     /locus_tag="VLN25_08320"
     CDS             complement(936..1391)
                     /gene="fur"
                     /locus_tag="VLN25_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HSQ10606.1"
                     /translation="MPNRSSTSSDLKNIGLKATVPRLKVLEIFQRSTAQRGVRHMSAE
                     DVYKALIAEHIDVGLATVYRVLTQFEQAGLLSRNHFEGGRAMFELNEGSHHDHLVCLT
                     CGRVEEFVDSDIEARQQQVAAQRGFKLHDHALALYGECTKVNCEYRPKE"
     gene            1452..1991
                     /locus_tag="VLN25_08325"
     CDS             1452..1991
                     /locus_tag="VLN25_08325"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HSQ10607.1"
                     /translation="MPHEYHTASMSNDRCPEPSQAPRRLLSVLAAATMLAGCQTLSDV
                     GSAAASWGPTFLGPYRPDVHQGNIITKEMVDQLRIGMTREQVRFMLGTPLMTSEFRKD
                     RVDYIYYLNPRRGPVQNRRLTLYFQDNRLDKFNADPMPGEDEADTLILGPDVKRVARR
                     SNQPVVPMPDVSTPPPSSN"
     gene            2038..2835
                     /gene="dapB"
                     /locus_tag="VLN25_08330"
     CDS             2038..2835
                     /gene="dapB"
                     /locus_tag="VLN25_08330"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HSQ10608.1"
                     /translation="MKLAIAGASGRMGQTLIEAALTQPDLSVSVALDRADSPMLGRDC
                     AETMGRSTGARVTSELSALAGADVLIDFTRPDATLRHLDACVAAHVKMVIGTTGFDDA
                     SKRAIQAASQRIAIVFAPNMSVGVNATFKLVEMAASMLGEGYDIEIIEAHHQHKVDAP
                     SGTALKMGEIVAAALGRPLAELAVYAREGHTGERRAGSIGFASIRGGDIVGDHTVMFA
                     GAGERIEITHRSGSRSSYANGGLRAARFLADKTTGLYDMFDVLGLAR"
     gene            2832..3515
                     /locus_tag="VLN25_08335"
     CDS             2832..3515
                     /locus_tag="VLN25_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HSQ10609.1"
                     /translation="MNNALGLAHFWELADGVIRFTAYLLLGMSVVSWFLILWKTWGWW
                     RVRRASKELDAFWSAPTIEVAIATLKPLDAEGVFVPLAVGASNAAIHPAAENSLAAQI
                     DRSELITRALRQRINEAAGRLEAGLTILASVGSTAPFIGLFGTVWGIYRALINIASSG
                     QIAIDKVAGPVGEALIMTAFGLAVAIPAVLAYNAFTRANRMVLGQLDGFAHDLHATLT
                     TGSRLAAQN"
     gene            3528..>3957
                     /locus_tag="VLN25_08340"
     CDS             3528..>3957
                     /locus_tag="VLN25_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HSQ10610.1"
                     /translation="MPFGGFERHGGTHQPMAEINVTPLVDVMLVLLVIFIITAPLLSY
                     AIRLDLPNDAAPAAELVPATVKLSIDAAGQIYWDADALTDDQLRSRLAGAGQQSTPPE
                     LHLRADKATRYERIAFVLSTAQQAGLTKIGFITEPAPGASP"
BASE COUNT          684 a         1295 c         1277 g          701 t
ORIGIN      
        1 aggatggtgg ccgggctgga ggaaatttcc ggcggcacga tccggatcgg cgagcgcatc
       61 gtcaatgatc tcgagccgaa ggatcgcgac tgcgcgatgg tgtttcagaa ctacgcgctg
      121 tatccgcaca tgagtgtcta cgacaacatg gcctatggtc tgaagatccg caagctgcct
      181 aaacctgaca tcgaacggcg cgttcaccag gccgcgggga tcctcgaact tgaagagctg
      241 ctgcagcgca agccgcgcca gctgtcaggg ggccagcgtc agcgcgtagc aatgggccgt
      301 gccatcgtgc gcgaaccggc ggtgttcctg ttcgatgagc cgctgtcgaa tctcgacgcc
      361 aaactgcgcg tgcagacccg gctcgagatc cagaagctgc accgccggct gggtacgacg
      421 agcctgtatg tcacgcacga ccaggtggag gcgatgaccc tcgcgcagcg catgatcgtg
      481 atgaacgccg gccgcgcaga gcaggtgggc accccggacg atgtctactc tcgcccggcg
      541 acgacctttg tcgccagttt catcggatcg ccgccgatga atctgttcga aggtcgtgtc
      601 gtggccgatg gacattcgtt tgaaaccaaa gatggagtgc gcctggccct gccggctgca
      661 gtcagtgcac tggccgggcg agtactcgtc atgggcgtgc gtcccgagca cctgaacctg
      721 ggtggcgagg ggctgacgat ggtcgtcgaa accgtggagc ggctgggggc cgaacacctg
      781 atccacggtg tagttgccgg ccatgatgtc atcgtccgga ccggtccgga cgattcaccc
      841 gcgcccggat cgaacctcag cctcggattt cctgccagcg cgatccattg gttcgatgcg
      901 tcaacgcggc agcggattgc cgtctgacgt cggccctatt ccttgggccg atactcgcag
      961 ttcaccttgg tgcattcgcc gtacagggcg agtgcatggt cgtgcagctt gaatccacgc
     1021 tgagcggcga cctgctgctg tcgcgcttcg atgtccgagt cgacgaactc ctcgacccgc
     1081 ccgcaggtca ggcacacgag gtgatcgtgg tgcgatccct cgttgagttc gaacatcgcg
     1141 cgaccgccct cgaagtgatt gcgtgacagc agcccggcct gctcgaattg cgtcagcacc
     1201 cggtagacgg tcgccaggcc gacatcgatg tgttcggcga tcagggcctt gtatacgtcc
     1261 tcggcgctca tgtgacggac accgcgttgc gcggtgctgc gctggaagat ttccagcacc
     1321 ttgaggcgcg gcaccgtcgc cttgaggccg atgttcttca ggtcgctgga cgtggacgag
     1381 cggttcggca tatcaccagg atgcagcaga cggatcgcag ttgcgcagtt ggagagccgg
     1441 cagagtcagg catgccgcat gagtatcata cggcctcgat gtctaatgac cgttgccccg
     1501 agccctcgca ggcgcctcgc cgcctgttgt ccgtgctcgc cgccgcaacg atgctggccg
     1561 gctgccagac cttgtccgac gtgggctcgg ccgcggcatc ctgggggccg acgtttctcg
     1621 gtccgtaccg tcccgacgtt caccagggca acatcatcac caaggaaatg gtggaccagt
     1681 tgcgtatcgg catgacgcgc gagcaggtgc gtttcatgct gggaacaccg ctgatgacaa
     1741 gcgagttccg caaggaccgc gtggactaca tctattacct gaatccgcgg cgcgggccag
     1801 tgcagaaccg gcgcctgacg ctctacttcc aagacaatcg actcgacaag ttcaatgccg
     1861 atccgatgcc gggtgaagac gaggccgaca cgctgattct cggcccggac gtcaagcgcg
     1921 tggcccgccg ttcgaaccag ccggtggttc cgatgccgga tgtctcgacg ccgcctccga
     1981 gctcgaactg atcttcccca cgtccaaacg ttctcccacc ccacggtcct caacccgatg
     2041 aagcttgcga tcgccggcgc atccggccgg atgggccaga cgctgatcga agcagccctg
     2101 acgcagccgg atctctcggt cagcgtggcg ctcgatcggg ccgacagccc gatgctcggc
     2161 cgcgactgtg ccgagacgat gggccggtcc accggagcgc gcgtcacgtc cgaactttcg
     2221 gcactggcgg gcgccgatgt attgatcgac ttcacccggc ccgacgcaac gctgcgtcac
     2281 cttgatgcgt gtgtcgccgc ccatgtgaag atggtgatcg gcaccaccgg attcgatgac
     2341 gccagcaagc gagcgataca ggccgcgagc cagcggattg caatcgtctt tgcaccgaac
     2401 atgagtgtcg gtgtcaacgc gaccttcaag ctggtcgaga tggccgcgag catgctcggc
     2461 gagggctacg acatcgagat catcgaagcc caccaccagc acaaggtcga tgcgccgtcg
     2521 ggaacggctc tgaaaatggg tgaaatcgtc gctgcggcgc tcggccgccc gcttgccgaa
     2581 cttgccgtgt atgcgcgtga aggacacacc ggggaacgcc gtgcgggatc aatcgggttc
     2641 gcgtcgatac gcggcggcga catcgtcggt gaccacacgg tgatgttcgc cggggccggc
     2701 gagcggatcg agatcacgca tcgctcgggc agccgctcct cgtacgccaa cggcggcctg
     2761 cgcgccgcgc gcttcctcgc cgacaagacg accggcctgt acgacatgtt cgacgtcctg
     2821 ggtctggcac gatgaacaat gcactgggcc tggctcactt ctgggagctg gcggacggcg
     2881 tcatccgttt cacggcctac ctgctgctcg gcatgtcggt cgtcagctgg ttcctgatcc
     2941 tctggaagac ctggggctgg tggcgcgtgc gccgcgcctc gaaggaactc gacgccttct
     3001 ggagcgcgcc gacgatcgag gttgcaatcg ccactctcaa gccgctcgat gccgaaggcg
     3061 tgttcgtgcc gctggcggtc ggtgcttcga atgccgcgat ccatccggcc gccgaaaata
     3121 gcctggcggc gcagatcgac cgctcggaac tgatcacgcg cgccttacgc cagcgcatca
     3181 atgaggcagc cggtcggctc gaagccggcc tcaccatcct ggcctcggtc ggcagcaccg
     3241 caccgttcat cggtctcttc ggcacggtct ggggcatcta ccgtgcgttg atcaacatcg
     3301 cctcgtcggg gcagatcgcg atcgacaagg tcgccggtcc ggtgggcgag gcgctgatca
     3361 tgacggcgtt cggactggcc gtcgcgatcc cggccgtgct ggcctacaac gcgttcacgc
     3421 gcgccaaccg gatggtgctc ggccagctcg acggcttcgc ccatgacctg catgcgacgc
     3481 tgacgaccgg ctcgcgcctg gccgcgcaga actgacgcgc cacgcagatg ccattcggag
     3541 gctttgaacg gcacggcggc acgcatcagc cgatggcgga gatcaacgtc acgccgctgg
     3601 tcgacgtcat gctcgtgctg ctggtgatct tcatcatcac ggcaccgctg ctgtcttacg
     3661 cgatccggct cgatctgccg aacgacgcgg cgccggcggc cgaactcgtc ccggcgacgg
     3721 tcaagctgtc gatcgatgcc gccgggcaga tctactggga cgccgacgcg ctgaccgacg
     3781 accagttgcg ctcgcgcctc gccggcgccg ggcagcagtc cactccgcct gaactgcacc
     3841 tgcgcgccga caaggccaca cgctacgaac gcattgcttt cgtgctgtcg acggcgcagc
     3901 aggccggact gacgaagatc ggcttcatca cggagcccgc gcccggcgcg tcgccgg
//
LOCUS       DATIRT010000369         3436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1437370, whole genome shotgun sequence.
ACCESSION   DATIRT010000369 DATIRT010000000
VERSION     DATIRT010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437370"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1640)
                     /locus_tag="VLN25_08345"
     CDS             complement(<1..1640)
                     /locus_tag="VLN25_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10611.1"
                     /translation="MALNVSMIYQRTDRGVREVYEKSHQFTQSERLILILLDGRLNVA
                     GLKTRLPSLNDERIERAMRKLQDAGLVEPMGSAEAMRVNGSAASDGRLEPEAVAQFLE
                     QSDLDPITIFGDSDEAVASELVRARVEQAAAQLAREATAPSRAAAAQRQVTAPRADPT
                     VSMRSHMPHSSPDRTTKSPDWEAATHFAITTVNVPDRLDLQEERDRADGEELADEQRR
                     QSLRKWMKRLLILAALGVAGTAVYSTIQPLQREVSAARVAERLTAVFKRPVTVADTEF
                     RFTPSPRMVVRGVETGTIKIDEASLLINWKDLWSALRGGQWVWGEATIAPMSVTSAQA
                     AEIATSLPAGANALSTKISTIRFESVRVSDTELLSGRYEAVIRRGDDGRFGPLVVREL
                     ESEGTMRLALRPAQTSDGRPVLDFRLDADRWTLPFGPKVRWNEVRANGRVDGKLIEVS
                     SYSLAGFYGVVSGMMYAASDIEWVITGRAIGTNIDLESVVQHLRKAPAGQEPILSPMQ
                     GTAALNFTVVGRGTTLDEAVQQAVFAGPFQLRWAALNG"
     gene            complement(1827..2822)
                     /locus_tag="VLN25_08350"
     CDS             complement(1827..2822)
                     /locus_tag="VLN25_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSQ10612.1"
                     /translation="MPREFILYAVLLAVVAALPFFGAYPVFVMKVMCYALFACAFNLL
                     IGFTGLLSFGHAAFLGGAAYAAGHALKVWGAPTELGLVVGVVAGAALGLVMGMLAIRR
                     SGIYFSMITLALAQMLYFFFLQAGFTGGEDGLQSVPRGTLLGIVNLESDVTLYYVVLG
                     IFVLSFLLIFRTVHSPFGQVLKAIRENEARATSLGYDTARFKLLAFVLSAALAGLAGA
                     TKTLVLGFATLTDAHWSTSGLVILMVLMGGMGTMLGPILGAFIVVALENKVGDIGMFL
                     GRVTGIEWFNGLGEAVTVVIGLIFVVCVMAFRRGLVGELNAWWTHRQAARAAGTA"
     gene            complement(2830..>3436)
                     /locus_tag="VLN25_08355"
     CDS             complement(2830..>3436)
                     /locus_tag="VLN25_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSQ10613.1"
                     /translation="LYKLDHLYGLLLTFGLTLLIEGFFRSFYGVSGLPYQVPELLEGA
                     TNLGFMIMPNYRGWVVLASLVVCLGTWYVIERTKLGALLRAGTENAKLTEAFGVNVPL
                     MVTLTYVFGVALAGFAGVLAAPILQVQPLMGSNLIIVVFAVVVIGGMGSILGSILTGL
                     GLGVIEGFTKVFYPEASATVVFLIMAIVLLIRPAGLFGKEK"
BASE COUNT          690 a         1146 c         1043 g          557 t
ORIGIN      
        1 atgccgttaa gtgccgccca tcgcagttgg aatggtcccg caaagaccgc ttgctgaacc
       61 gcttcgtcaa gcgttgtgcc gcgcccgacc accgtaaagt tcaatgccgc cgtgccctgc
      121 atcggggaaa ggatgggttc ctgtcccgcg ggcgccttgc gcagatgctg gacgacggat
      181 tcgaggtcga tgttcgttcc gatagcgcgt ccggtgatga cccactcgat atcgctggcc
      241 gcgtacatca tgccgctgac cacgccgtaa aaacccgcca gcgaatagct cgacacctcg
      301 atcagtttgc cgtcgacccg accgttcgcc cggacctcgt tccatctgac cttcggaccg
      361 aaaggcagag tccaccgatc ggcgtcgagt cgaaagtcga ggacaggccg cccgtcgctc
      421 gtctgggccg ggcgcaacgc gaggcgcatc gtcccttccg attcgagctc acgcaccacc
      481 agggggccga atcgaccatc atcaccgcgg cggatcacgg cctcgtatcg gcccgacagc
      541 aactcggtat ccgagacccg aacggattcg aaccggatcg ttgaaatctt ggtcgagagt
      601 gcgttggcac cggccggcaa tgacgtcgca atttctgccg cctgcgcaga tgtgacgctc
      661 atcggtgcga tcgtggcttc gccccagacc cactgcccgc cccgcaacgc actccagagg
      721 tccttccagt tgatcagcag ggacgcctcg tcgatcttga tggtcccggt ctcgactccc
      781 cgaaccacca tgcgcggcga gggcgtaaat cgaaactcag tgtctgccac agtgaccggc
      841 cgcttgaaga ctgccgtcag ccgttccgcc acacgggcag cgctcacctc ccgctgcagt
      901 ggctggatcg tgctgtacac ggccgtcccc gcgacgccga gggcggcgag gatcagcagg
      961 cgcttcatcc acttgcgcag tgattggcgg cgctgctcgt cggcgagttc ttcgccatcg
     1021 gcccggtcgc gctcctcttg cagatcgagc cggtccggga cattcacggt cgtgatcgca
     1081 aagtgggtgg ccgcctccca gtcgggcgat ttggtggtgc ggtccggcga tgaatgcggc
     1141 atatgtgagc gcatactcac cgttggatcg gctcgtggcg cggtcacctg ccgctgggcc
     1201 gccgcggcgc gcgaaggcgc ggttgcttcg cgagccagtt gtgcagccgc ctgctcgacg
     1261 cgagcgcgca ctaattcgct ggcgactgct tcatcggagt cgccgaaaat cgtgatcgga
     1321 tcaagatcgc tctgctcgag gaactgcgca acagcttccg gttcgagccg cccgtccgat
     1381 gcggcggacc cattgacacg catcgcctcg gccgaaccca tgggctcgac caggccggca
     1441 tcctgcagct tgcgcattgc gcgctcaatg cgctcatcgt tgagactcgg cagacgtgtc
     1501 ttgagtccag cgacgttcag ccggccgtcg agcaggatga gtatcagccg ctcgctctga
     1561 gtgaactggt gcgacttctc atagacctcg cgcacaccgc gatcggtgcg ctgatagatc
     1621 atcgaaacgt taagcgccat gggaagtcgt gcgggctccg cgcggcggag tcatcgtcac
     1681 agatcaatcg attgtatcta tgaagcccgc tgattcacac ggcggcgttc atccgataag
     1741 cgggaaaatc gcgcggtagc gaaagcgtac cgcgcctgca ggggcactac ctgtaacgcc
     1801 ctaaggcggc gactgacgct gatgcgctag gccgtgccgg ctgcgcgagc tgcctgccgg
     1861 tgcgtccacc aggcattgag ttcaccgacc agaccgcgcc ggaacgccat cacgcacacg
     1921 acgaagatca gcccgatcac gaccgtgacc gcctcgccca ggccgttgaa ccactcgatg
     1981 ccggtcacgc gacccaggaa catgccgatg tcgccgacct tgttctcgag cgcgacaacg
     2041 atgaatgcgc cgaggatcgg gccgagcatc gtgcccattc cgcccatcag caccatcagg
     2101 atcacgagtc ccgacgtact ccagtgcgca tcggtcagtg ttgcaaagcc aagcaccagc
     2161 gtcttggtcg ccccggccaa tccggcaagg gcggccgaaa gcacgaacgc cagcagcttg
     2221 aagcgcgccg tgtcgtagcc gagcgacgtc gcgcgcgctt cattctcgcg gatcgctttc
     2281 aacacctggc cgaaagggga gtgcaccgtg cggaagatca gcaggaaaga cagcacgaag
     2341 atgcccagga ccacgtaata cagcgtgacg tcgctctcga ggttaacgat tcccagcagc
     2401 gtgccgcgcg gtaccgactg caggccgtct tcccccccgg tgaatcccgc ttgcaggaag
     2461 aagaagtaca gcatctgcgc aagtgcgagc gtgatcatcg aaaagtaaat gccgctgcgc
     2521 cggatcgcca gcatccccat cacgagaccg agcgccgcgc cggcgaccac gccaaccacg
     2581 agacccagct cggtgggcgc tccccagacc ttgagtgcat gccccgccgc atacgccgcg
     2641 ccgccgagaa acgccgcatg accaaatgac agcagcccgg tgaatccgat cagcagattg
     2701 aacgcgcacg caaagagcgc gtagcacatg accttcatca caaagaccgg atacgcaccg
     2761 aaaaacggaa gcgctgcgac gaccgcgagc aggaccgcgt acaaaatgaa ctcgcgcggc
     2821 agcttcacgc tatttctcct tgccgaagag gcccgccggg cggatcagca gcacgatcgc
     2881 catgatcagg aacacgaccg tggcagaggc ctctggatag aagactttgg tgaagccctc
     2941 gatgacgcca aggcccaggc ccgtcaggat cgagccgagg atcgacccca tgccgccgat
     3001 gacgacgacg gcgaacacga cgatgatcag attgctgccc atcaatggct gcacctgcag
     3061 gatcggcgcg gcgagcacgc cggcgaaccc tgccagcgcc acgccgaaga cgtacgtcag
     3121 cgtgaccatc agcggtacgt taacgccgaa cgcctcggtc aacttggcgt tctcggtgcc
     3181 tgcgcgcagc agcgcgccga gcttggtgcg ctcgatcacg taccacgtcc cgaggcagac
     3241 cacgagcgac gccagcacga cccagccgcg gtagttcggc atgatcatga agccgagatt
     3301 ggtggcgcct tccagcagct cgggcacctg gtacggcagg ccggacaccc cgtagaagga
     3361 gcggaagaac ccttcgatca gcagcgtcag gccgaatgtc agcaacaggc cgtagaggtg
     3421 gtcgagcttg tacaga
//
LOCUS       DATIRT010000370         4375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_945665, whole genome shotgun sequence.
ACCESSION   DATIRT010000370 DATIRT010000000
VERSION     DATIRT010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945665"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..600)
                     /locus_tag="VLN25_08360"
     CDS             complement(115..600)
                     /locus_tag="VLN25_08360"
                     /inference="COORDINATES: protein motif:HMM:NF020551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtaL-like protein"
                     /protein_id="HSQ10614.1"
                     /translation="MRFEHLVQINDPLMPLLTEVSRAQLWRGLVLRAEDPTHFVVGLV
                     GAAIDARRELSGVTELERTLDYGSFKVRDRVRLMPEHRTEIRTAAGESWPASRMSITI
                     EEPDPEALFLRFVYEADSEIDGKGELDEMTVTLRQQAYEGADLDTVVRIRALAEAGTL
                     G"
     gene            762..1592
                     /locus_tag="VLN25_08365"
     CDS             762..1592
                     /locus_tag="VLN25_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10615.1"
                     /translation="MLTRWLRSTPACKGTLFAAACTAMLALASPAHAVGSLVDVQIMD
                     RATGETLPMYWHHGRWWVPGKPGSRYAVALSNRAGGRALAVVSVDGVNVVSGDTAAHD
                     QVGYVLRHGQFAQITGWRKDMARVAAFEFTALNDSYAARTGRPDNVGVIGVAVFRERA
                     RPVPAPASPPLSKLESARDGAAPAAGASAANESDARQRADAAPTREQRLGTGHGRNER
                     SPTSYTDFERAQSTPNELIAIHYDSRDNLIAMGVIPTPRAVPDPFPAAVGFVADPPLR
                     "
     gene            1697..2266
                     /locus_tag="VLN25_08370"
     CDS             1697..2266
                     /locus_tag="VLN25_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSQ10616.1"
                     /translation="MSAAPTDEELLTAYAAGDARAFGELYERHERPVYRYFLRQGVVA
                     AAADDLLQETWMAVIRNAVRFEPRAKFTTWLYTVARSKMIDHWRARDDAISFDDAAND
                     PDDTPFDVAGSDADRPDMQALSRAQARAFVDAVEALPAVQREAFLLQAEGGLSLEDIA
                     SITQAGHETVKSRLRYAMTKLRAAMEEWR"
     gene            2263..3312
                     /locus_tag="VLN25_08375"
     CDS             2263..3312
                     /locus_tag="VLN25_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10617.1"
                     /translation="MSGTGDESLDRGYRAANDALDERPSAATRAAILAAAARQVQAGP
                     RDAQAPRGASLFGTTLRWPMAAAATVLLSTLAVMMAVRTDQEMPSFSPPAEIEPRVTR
                     APDSPSAAASSARQSAPVGSDVGGLAAPSTAMSADSAAPSAERKRALAKEGVVAAPPI
                     AVPPARTAPESAPSSPPSARDQATANAQADAKVTAAAQGARAKEEVAAQFSPAVPSAP
                     SPAREASRSNDSAPDATVPAAPLPQAKADAAQPTDTESRRNGRADSSGPALSKLRQAP
                     APTGAPGAGVGTSRSEVDARTDDTLRAEDWLEKIIKLRKAGRHDEADAELKRFRERYP
                     HVQVPVDALPATGTR"
     gene            3330..3722
                     /locus_tag="VLN25_08380"
     CDS             3330..3722
                     /locus_tag="VLN25_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10618.1"
                     /translation="MTTVPSHVVLLDSITQVDARHAGLCVVTGSHGGVSAARFASAVA
                     ARLYVFNDAGVGKDAAGIAALALLDVRGIAAVTVAHDSARIGEARDTWDNGVISHGNA
                     TAGRIGVQAGARLGEALAVLPGEPSDDG"
     gene            complement(3745..4137)
                     /locus_tag="VLN25_08385"
     CDS             complement(3745..4137)
                     /locus_tag="VLN25_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008344674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HSQ10619.1"
                     /translation="MKNAYGFGKTVALGFDEAVQKVTSELAREGFGVLTEIDVQSTLK
                     KKLDVDVRPYRILGACNPPLARRALDAEPTIGLLLPCNVVVRESDDGRTQVEFMDPAT
                     MQALSESAALGEVATEVRARLVRVMEAV"
BASE COUNT          723 a         1492 c         1503 g          657 t
ORIGIN      
        1 ggcagcttct tccgtaccgc gcggcggctg ttcgaaggcc gcgtctgcct gcccgagtga
       61 acgtgcgccg gcccctcggg ggcagcggac gaagcgaacc ggggtccacc gcgctcagcc
      121 cagtgttccg gcctctgcca acgcgcggat gcgcaccacg gtgtcgaggt cggcgccttc
      181 gtaggcctgc tgccgcaacg tgaccgtcat ctcgtccagc tcgccttttc cgtcgatctc
      241 ggagtcggcc tcgtagacga agcgaaggaa gagcgcctca ggatcgggct cctcgatcgt
      301 gatcgacatc cggctggccg gccatgactc cccggcggcc gtccggatct cggtgcgatg
      361 ctccggcatc agacgcacgc ggtcgcgcac cttgaagctg ccgtagtcga gcgtgcgttc
      421 gagttcggtg acgccactga gttcgcgccg cgcatcgatc gccgcaccaa ccagtccgac
      481 cacgaagtgg gtcgggtcct ccgcccgcag caccagtcca cgccagagct gcgcgcgcga
      541 gacctccgtc agcagcggca tcagcggatc gttgatctgg acgaggtgtt cgaagcgcat
      601 gccgaactca gtcgcagggg aaggaaccct gcggtccgag cagggatcgg atctcgatcg
      661 atctcgtggt caggccggtc atcggtcggg ggcgggcgtt taaccccatt gtctccgaag
      721 cggcgtttcc agcgtatcag ccacccacca ggagaccttc gatgctgacc cgctggcttc
      781 gttccacccc tgcctgcaag ggcaccttgt tcgccgccgc atgcaccgcg atgctggcac
      841 tggcttcgcc tgcacatgcg gtcggctcgc tcgtcgacgt gcagatcatg gaccgcgcga
      901 ccggcgagac actgccgatg tactggcacc acggccgctg gtgggtgccc ggcaagcccg
      961 gcagccgcta tgccgtcgcg ctgagcaacc gcgcaggtgg gcgggcgctg gcggtggttt
     1021 ccgtcgacgg tgtcaatgtc gtcagcggcg acaccgcggc acacgaccag gtcggctacg
     1081 tgctcaggca cgggcagttc gcgcagatca ccggctggcg caaggatatg gcgcgcgtgg
     1141 ccgcgttcga gttcactgcg ctcaatgact cgtatgccgc gcggacaggc cggccggaca
     1201 acgtcggcgt catcggcgtg gccgtgttcc gcgaacgcgc gcgccctgtg cctgcgcccg
     1261 cgtcgccgcc gctatccaag ctggagtcgg cccgtgacgg tgccgccccg gcggctggcg
     1321 cgagcgcggc caacgaatcg gacgcgcgtc agcgagccga tgccgcaccg acgcgtgagc
     1381 agcgactcgg caccggacac ggacgcaacg aacggtcgcc gacgtcgtac accgacttcg
     1441 agcgcgcgca gtcgacgccg aacgagctga tcgcgatcca ctacgacagc cgcgataacc
     1501 tgatcgcgat gggcgtgatc ccgacgccgc gtgccgtgcc ggatccgttc ccagcggcgg
     1561 tggggttcgt ggccgacccg cccctgcgct gagccctgcg ttggcggttc agctcccaat
     1621 cgcggcgatc ggacggcggc cgttgcgggc acaatcgccc gcgccatgcg ctgccacgcc
     1681 gcgacaggga gcacgattga gcgctgcgcc gaccgatgaa gagttactta ccgcctatgc
     1741 ggcgggcgac gcgcgcgcct tcggtgaact gtacgagcgg cacgagcgcc cggtgtatcg
     1801 ctactttctg cgccaggggg tggttgcggc cgcggccgac gacctgctgc aggagacctg
     1861 gatggcggtg atccgaaatg ccgtgcgctt cgagccaagg gctaaattca cgacctggct
     1921 ctatacggtg gcacgcagca agatgatcga tcattggcgt gcgcgcgacg acgcgatcag
     1981 cttcgacgac gcggccaacg accccgacga cacgcccttc gatgtggccg gttccgacgc
     2041 cgaccggccc gacatgcagg ccctgtcgcg ggcgcaggcc cgggcgttcg tcgatgcggt
     2101 ggaggcactg cccgcggtgc agcgcgaagc gttcctgctg caggccgagg gcggtttgtc
     2161 actcgaggac attgcgtcga tcacgcaggc ggggcacgaa acagtcaaga gccgcctgcg
     2221 ctacgcgatg acgaagctgc gggcagcgat ggaggaatgg agatgagcgg cactggggac
     2281 gaaagcctcg atcgcggcta ccgcgccgcg aacgacgcgc tcgacgagcg tccatcggcc
     2341 gcaacccgcg cagcgatcct cgcggcggcg gcgcgccagg tgcaggcagg cccgcgtgac
     2401 gcacaggccc cacggggagc gtcgcttttc ggaacgacgc tgcgctggcc gatggccgcc
     2461 gcggcgacgg tgctgctgtc gacgctggcc gtgatgatgg ccgtgcgaac cgaccaggaa
     2521 atgccgagct tcagtccgcc cgccgagatc gagccgcgag tgacaagggc accggactca
     2581 ccctcggcag cggcgtcgtc agcgcgtcag agcgcgccgg tggggagcga tgtgggcggg
     2641 ttggccgcgc catcgacggc gatgagcgcg gattccgccg caccttccgc cgagcgcaag
     2701 cgcgcactgg ccaaggaagg cgtggtggcg gcgccgccga ttgccgtgcc gcctgcaagg
     2761 acagcgccgg aatcggcgcc atcgagtcct ccgtcagcgc gcgaccaggc aaccgcgaat
     2821 gcgcaggccg acgccaaggt gacggccgcg gcccaaggcg cacgcgcgaa ggaagaggtt
     2881 gcagcgcagt tttcaccggc ggtgccgtcc gcaccgtcgc cggcgcgcga ggcatcccgg
     2941 agcaacgact cggcgcccga cgcgactgtt cctgcggcac ccctgcccca ggccaaagcg
     3001 gacgccgcgc agccgacgga cacagagagt cggcgcaatg ggcgggctga cagctcgggg
     3061 ccggcacttt caaagctgcg gcaggcaccg gcgccgaccg gcgcacccgg cgccggcgtc
     3121 ggcacttcac gcagtgaggt ggatgcacgg accgacgaca cgctgcgcgc tgaggattgg
     3181 ctcgagaaaa tcatcaagct gcgcaaggcc ggccggcacg acgaagcgga tgcggaactc
     3241 aagcgctttc gcgaacgtta tccgcacgtg caagtcccgg tggatgcatt gccggcgacc
     3301 ggaacacgct agattcgtac ggtaggcgcg tgacaacagt cccctcccat gtcgtgctgc
     3361 tcgactcgat cacgcaggtc gatgcgcggc atgccgggct ctgtgtggtc actggatcgc
     3421 acgggggcgt gtcggcagcg cgcttcgcga gtgcggtcgc ggcgcggctc tatgtcttca
     3481 acgatgccgg ggtcggcaag gatgcggccg gaattgcggc cctcgcgctt ctcgacgtgc
     3541 gtggcattgc agcggtgacg gtggcgcatg attcagcgcg catcggcgag gcccgcgata
     3601 cctgggataa cggcgtcatc tcgcacggca atgccacggc cggacgtatc ggagtacagg
     3661 ccggcgcacg actcggcgag gcgctcgctg tgttgcccgg cgaacccagc gacgacggct
     3721 gagcacccac ggtccggcga cggctcagac tgcctccatc acccgcacaa gcctcgctcg
     3781 tacttcggtc gcaacttcgc ccagcgcggc gctctcggac aacgcctgca ttgttgccgg
     3841 atccatgaac tcgacctgcg tgcggccatc gtcggattca cgcacgacca cattgcacgg
     3901 cagcagcagc ccgatggtcg gctcggcgtc gagcgcacgg cgagccagtg gcgggttgca
     3961 ggcgccgagg atgcggtaag gccgcacgtc gacgtcgagc ttcttcttca aagtggactg
     4021 cacgtcgatc tccgtcagca cgccgaaacc ttcgcgtgcg agttctgacg ttactttctg
     4081 cacggcctcg tcaaagccaa gtgcgaccgt cttgccgaaa ccataggcgt tcttcatcgc
     4141 tgtcctgttg cacgaaacct gtcgtacgaa acctgaagta ggaaaccttc agtacgaacc
     4201 ctaagactca tgatgccgtg gccgcggcca gctcgcattg agccgtgaca agcgacacgc
     4261 actccacggc cggctttccg tcgagtgcct gcagggccag ccgcgcggca tcggcttcaa
     4321 agatgcgcgg cgcggtccgc gccagcgcca gcgccgcgtc gagcgtcgag accga
//
LOCUS       DATIRT010000371         3068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1044826, whole genome shotgun sequence.
ACCESSION   DATIRT010000371 DATIRT010000000
VERSION     DATIRT010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044826"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VLN25_08390"
     CDS             complement(<1..469)
                     /locus_tag="VLN25_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HSQ10620.1"
                     /translation="MQIILVDRRMARARTITVRAQHLVAAVVLLTLSIVVVSTVFSLL
                     TVRAASALPLPFVSDVIAFVTRDQAERKDQYVRDNVSAMTRQLGEVQAQLLRLDVLGE
                     RVAKAAGIRPEEFRFRELPGRGGAPGTTMSFTDLESELQGLTRGVEQRADYLAV"
     gene            524..1021
                     /locus_tag="VLN25_08395"
     CDS             524..1021
                     /locus_tag="VLN25_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10621.1"
                     /translation="MSGPARTVAAALRDSPDVAALLARWETAQAVSRVLAPLAHSLAP
                     GMDLFVPGRCDLREGVLWITVESAAESAKLRQAAPRFLAALASHGFQVYEMKSRVQPG
                     GTSYPGQGMTGASSPGLAFAPVTDRGVGAVEAAARLLPESALQRALQRLAKTLGHRRD
                     APPTL"
     gene            complement(1018..1929)
                     /gene="lpxC"
                     /locus_tag="VLN25_08400"
     CDS             complement(1018..1929)
                     /gene="lpxC"
                     /locus_tag="VLN25_08400"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HSQ10622.1"
                     /translation="MLKQRTLKQLARTVGIGLHSGTKVELVMRPAAADTGIVFRRVDL
                     DPPVEIRLTPEAVVDTRMATTIGRDAVRIATIEHLMSALAGLGIDNCYIDVDAAEIPI
                     MDGSASSFVFLLQAAGIEELNAPKKFLRVLKPVEIVDGDKWARLEPYFGYRLKFSIDF
                     KHPAIDSTEQVVEVDFAHDSYVQQVSRARTFGFVNEVEALRAAGLAMGGTFENAIVMD
                     EYRVLNTDGLRAGDEFAKHKILDAIGDLSALGRPLMASYVAHKSGHAINNRLLRALVA
                     DAAAYDLVTFEDDAEAPAAFLTQLQPA"
     gene            2110..2865
                     /locus_tag="VLN25_08405"
     CDS             2110..2865
                     /locus_tag="VLN25_08405"
                     /inference="COORDINATES: protein motif:HMM:NF021472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HSQ10623.1"
                     /translation="MSPLAPFTWLARGAHDLRRAPAASLAYGLAFALMGGLIVVVFRN
                     AYELTSALVAGFLLVGPFLATGLYDISRRLERGSRVRLRETTTAWRANLGAFSLFALA
                     LTIIALVWARASLVTFALFFSGGMPTLKSFLAQIVSVENIDFLLTYFAVGALFAGIVF
                     AVSVVSVPMMLDRGTDTIVAALTSVRALFANPLPLLIWALLIVVIIAAGFATFFVGLV
                     VAVPVIGHATWHAYRDLVVDGRDPSDRTCIDNG"
BASE COUNT          493 a         1039 c          966 g          570 t
ORIGIN      
        1 ccacggccag atagtcagcg cgctgctcga ctccgcgggt caggccctgc agttcggact
       61 ctagatcagt gaagctcata gtggttcccg gcgcgccgcc ccgtcccggc aactcgcgga
      121 aacggaactc ttcgggccgg atgccggcgg ccttggccac gcgctcgccg agcacatcga
      181 gccgcagcaa ctgcgcctgc acttcgccga gctggcgcgt catggccgaa acgttgtcgc
      241 gcacgtactg atccttgcgc tcggcctgat cgcgcgtcac gaaggcgata acatcggaaa
      301 cgaagggcaa gggcagcgcc gaggccgcac ggacggtcag cagggaaaag acggtggaca
      361 cgacgacgat cgacagcgta agcaggacga ccgccgcgac caggtgttgc gccctgaccg
      421 tgatcgtgcg tgctcgcgcc attcgccgat cgaccagaat gatctgcact gcctcctcct
      481 acccctgcga caccgcgccg cgaagcgccg cgtcgatcac ttgatgtccg gtcccgcacg
      541 cacggtcgcg gccgcactga gggactctcc ggacgtcgcc gcgttgctgg cccgttggga
      601 aaccgcacag gcggtcagcc gcgttctggc gcccctggcg cattctttgg ctcccggaat
      661 ggatctgttc gtccccggcc gctgcgacct gcgcgagggt gtcctgtgga tcaccgtcga
      721 gtcggccgcc gagtccgcca aacttcgcca ggccgctccg cgtttcctcg cagcactggc
      781 atctcatggg tttcaagtct acgagatgaa gtctcgcgta caacccggcg gcacgtccta
      841 ccccggacag ggtatgacgg gggcctcaag tccgggattg gcgtttgcgc cggttacgga
      901 ccggggggtt ggcgcggtcg aggccgccgc ccggctgttg ccggaatcag cgctgcagcg
      961 agcgctgcaa cgactggcga aaacgctggg tcatcgacgc gacgccccgc cgacgctcta
     1021 ggctggctgc agctgggtca ggaatgccgc tggcgcctcg gcgtcgtctt cgaaggtcac
     1081 caggtcgtag gccgcggcgt cggcaaccaa agcgcgcagc agccggttgt tgatcgcgtg
     1141 tccggatttg tgcgccacgt aactggccat caagggacgt ccgagcgccg acaggtcacc
     1201 gatggcatcg aggatcttgt gcttggcgaa ctcgtcgccg gcacgcagcc cgtcggtatt
     1261 cagcacgcga tattcgtcca tcacgatcgc gttctcgaaa gtgcccccca tcgccaaccc
     1321 ggccgcgcgc agggcctcga cttcattgac gaagccgaat gtccgcgcac gcgacacctg
     1381 ctggacatac gagtcgtgcg cgaaatccac ctcgaccacc tgctcggtcg agtcgatggc
     1441 cgggtgcttg aagtcaatcg agaacttcag gcgatagccg aaatagggtt caagtcgcgc
     1501 ccatttgtcg ccatcgacga tttcgaccgg cttgagtacg cgcaagaatt tcttgggcgc
     1561 gttgagttcc tcgatgccgg cggcctgcag caggaacacg aagctggacg cgctgccgtc
     1621 catgatcgga atttctgccg catcgacgtc gatgtagcag ttgtcgatcc ccaatccggc
     1681 cagggcactc atcaggtgct cgatggtggc gatgcgcacc gcatcgcggc cgatcgtcgt
     1741 cgccatgcgc gtgtcgacga ccgcttccgg cgtcagccgg atctcgaccg gcggatccag
     1801 atcgacgcgg cgaaacacga tgcccgtatc agcagcagcc ggacgcatga ccaactcgac
     1861 cttggtcccg ctgtgcaggc caatgcccac ggtacgggcc agctgcttca aggtgcgttg
     1921 cttcaacatg gcgtcattct atccgtcggc ttgtgcgctg cggcgagcgt cgtaaggctc
     1981 ggcagccgag aatccggcca tcgtcctgac ccacctggca gagccctcac cgtgtcgccc
     2041 gaaaccccgt cgtcggaaga ccgcagcgca gcggcggaca ccgcatcgcc cttccctgcc
     2101 attcgcaccg tgtccccctt ggcgccgttc acctggctgg ctcgcggcgc ccacgacctg
     2161 cgccgcgcac cggcggccag cctggcctat ggattggcgt ttgcattgat gggcgggctg
     2221 atcgttgtcg tgtttcggaa cgcctacgag ctcacgtcgg cgctggtggc aggcttcctg
     2281 ctcgtcggac cgttcctcgc gaccgggctg tacgacatca gccggcgcct ggaacgcggt
     2341 tctagggtgc ggctgcgtga gaccacgacg gcctggcgcg ccaacctggg cgcgttcagc
     2401 ctgttcgcgc tcgcgctgac catcatcgcg ctggtttggg cacgggcgtc gctggtgacc
     2461 tttgccttgt tcttttcggg tggcatgccg acgctgaaga gttttctcgc acagatcgtc
     2521 tcggtcgaga acatcgactt ccttctgacc tacttcgcgg tcggcgcgtt gtttgccggc
     2581 atcgtcttcg cagtcagcgt ggtgtccgtg ccgatgatgc tcgaccgcgg caccgacacc
     2641 atcgtggccg ccctgaccag tgtgcgcgcg ctctttgcca atccactgcc gctgctgata
     2701 tgggcgctgc tgatcgtcgt catcatcgcg gcaggttttg ccaccttctt tgtcggcctc
     2761 gtcgttgccg tgccagtgat cggacacgcg acgtggcatg cctacagaga tctcgtcgtc
     2821 gatggtcgcg atccttcgga tcgcacgtgc atcgacaacg gctaacaact gtcggctgcg
     2881 ctccggagcg gccgcgcgct tcgccgagtt tcgactgcac cgcacccact cgacctccca
     2941 ccgagcggga ggagaagtgc acccctccct caccctccct cctggagggg agggcaatga
     3001 cgaaacagca acaagcacgc tgcttccgga agtggggtgt ccgcctttgc ccttaggcgc
     3061 agcgcgcg
//
LOCUS       DATIRT010000372         3429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1537215, whole genome shotgun sequence.
ACCESSION   DATIRT010000372 DATIRT010000000
VERSION     DATIRT010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537215"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1624
                     /locus_tag="VLN25_08410"
     CDS             <1..1624
                     /locus_tag="VLN25_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSQ10624.1"
                     /translation="YEYDYLARPFRIVPSTAASMPEVSDDFRVWTVRIKPGTLFQDDP
                     AFKGKARELVAGDYVYSFKRFFDPRWKAPTYPTLAETRLLGMAALRDDALKTKRPFDY
                     DTPVDGIRALDRYTIQFRVERPLPRFLQILTGGDLYGAVAREVIEAYGDATPGHPVGT
                     GPFRLSDWRRSSKIVLVRNPTYREVVYDAEPAADDAEGQALLARFKGRKLPIIDRVEV
                     SIIEESQPRWLAFLNKQQDLLERLPFDFMNIAAPNGVLAPHLARQNIQLWRTLNADVT
                     LTYFNMADPVVGGYEPAKVALRRAIGLAINVDQEIRLFWRGQAVPAQSGLMPHTMGYD
                     ANFVNETARYDLPRAKALLDLYGYVDRDGDGWREQPDGSPLVLEWHTTPDQRARQRDE
                     LRRKDLSALGIRIDFKSAKWPENLKNARAGKLMVWNLGYSAASPDGQPSLDRGASIHH
                     GGQNLARYSNKKFDSIYDRIRVLPDGPEREELFREAKRLMAVDAPYRFHVHRILTDLA
                     WPWLIGFRRQLYSNTFWQYVDIDTDLQRRENT"
     gene            1621..>3429
                     /locus_tag="VLN25_08415"
     CDS             1621..>3429
                     /locus_tag="VLN25_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSQ10625.1"
                     /translation="MNEFPPDSPGRRTALKALAAAATGLPSLTVAQEPRRKVLRYAFQ
                     IAETGMDPVALSDLYSRILTAHIFENFYEYDHLARPFRIRPCTADGMPEISDDFRTCT
                     VRIKPGIFFQDDPVFKGKKRELVAQDYVYAWKRFFDPRWKAPAFATLNELNMPGLAAL
                     RDAVIKDKKPFDYDTEVDGMRAIDRYTVRFRFSEPQPRFIQTMTGGDLWGAVAREVVE
                     AYSDDIAAHPIGTGPFRLAEWRRSSRIVLERNPTYREVFYDAEPNADDAAGQALLAKY
                     KGHKLPILDRVEISVIEEQQPRWLSFLQKQQDLAWLLPNEFVNIAIPNNQLAPNLAKQ
                     GMQMYRVLASDVTLTVYNMDSPVIGGYTPDRVALRRALSLSLNTEQEIRLERKNQAIP
                     AQSPLVPNTMGFDPNYKSEGYNPQKAKALLDLYGYVDRDGDGFREQPDGSPLVIEIST
                     QPDQASRRLDELQRKDFTAVGIKTEFKQAKWPENLRSVRAGKYMVWRVGSSAASPDGQ
                     PALDRSASIHKGGQNLARFSNKQFDEIYRRLGVIPDGPERDQLFREAKRVVAAYAPYH
                     NGVHRILTDLAWPWLIGFRRPPYWLAWWQFVDVDAEQ"
BASE COUNT          659 a         1130 c         1053 g          587 t
ORIGIN      
        1 gtacgaatac gactacctgg cgcggccgtt caggatcgtt cccagcacag cggccagcat
       61 gcccgaggtc tcggacgact ttcgcgtctg gaccgtccgc atcaagccgg ggactttatt
      121 ccaggacgac cccgcgttca agggtaaggc gcgcgagctg gtcgcaggag actatgtcta
      181 ctcgttcaag cgcttcttcg accctcgctg gaaggcgccg acatacccga cgctggccga
      241 gacccgactg ctcgggatgg ccgcgctgcg cgatgacgcg ctgaagacca aacgcccgtt
      301 cgactacgac acgccggtcg acgggatacg ggcgctggac cgatacacga tccagttccg
      361 cgtggagcgg ccgctgccgc gttttctgca gatactgacc ggcggcgact tgtacggtgc
      421 cgtggctcgg gaagtgatcg aggcctatgg cgatgcaacg cctggtcacc ccgtcggtac
      481 cggcccattc cgactgagcg actggcgccg ctcttccaag atcgtgctgg tgcgcaatcc
      541 gacctaccgc gaagtcgtct acgacgccga acccgcggcc gacgacgcgg aggggcaggc
      601 gctgctggcg cgtttcaagg gccgcaagct gccgatcatt gatcgcgtcg aggtgtcgat
      661 catcgaggag tcgcagccgc gctggctggc gtttctgaac aagcaacagg acctgctcga
      721 gcggctgccg ttcgacttca tgaacatcgc cgcccccaat ggtgtcctgg ccccgcacct
      781 tgctcgccag aacatccagt tgtggcgcac gctgaatgcc gatgtcacgc tgacctattt
      841 caatatggcc gacccggtag tcggcggcta cgagccggcc aaagtcgcat tgcggcgcgc
      901 gatcggcctc gccatcaacg tggatcagga gattcgcctg ttctggcgcg ggcaggctgt
      961 gccggcccaa tccggcctga tgccgcacac gatgggctac gacgcgaact tcgtcaacga
     1021 aaccgcgcgc tacgacctgc cgcgagccaa ggcgctgctt gacctgtacg gatacgtcga
     1081 ccgcgacggc gacggctggc gcgaacaacc tgacggctcc cctctcgtgc tcgagtggca
     1141 cacgacgcca gaccagcgcg cacgccagcg cgatgaactt cgtcgcaagg acctctctgc
     1201 gctggggatc aggatcgact tcaagtccgc caagtggccg gagaacctga agaatgcgcg
     1261 ggccggcaag ctcatggtct ggaatctcgg ttactcggcg gcgtcacccg acgggcaacc
     1321 ctcgctcgac cgcggcgcca gcatccatca tggcggccag aatctggccc gatacagcaa
     1381 caagaagttt gactcgatct atgaccgcat ccgtgtccta cccgatggcc ccgaacgtga
     1441 agagctgttc cgggaagcca agcggttgat ggcggtcgat gcaccctatc gcttccacgt
     1501 gcaccgcatc ctgaccgacc tcgcctggcc gtggcttatc gggtttcgac gtcagcttta
     1561 ctcgaataca ttctggcagt acgtcgacat cgacacggac ctgcagcgca gagagaacac
     1621 atgaacgagt tcccccctga ctcccccggc cgtcgcaccg cactcaaggc cctggcagcc
     1681 gccgccaccg gcctgccttc gctgaccgta gcgcaggaac cgcggcgcaa ggttctccga
     1741 tacgcgttcc agattgccga gacgggcatg gatccggtcg cgctctcgga cctttactcc
     1801 cgcatcctga cggcacatat ctttgagaac ttctacgagt acgaccatct cgcgcgccca
     1861 ttcaggatcc ggccctgcac cgcggatggc atgcccgaga tctcggacga cttccgcacc
     1921 tgtaccgtgc ggatcaagcc tggcatcttc ttccaggacg atccggtatt caaaggcaag
     1981 aagcgcgaac tggtcgcgca ggactacgtg tacgcgtgga agcgcttctt cgacccgcgc
     2041 tggaaggcac cggccttcgc cacgctgaac gaattgaaca tgcccggtct ggcggcgctg
     2101 cgcgacgcgg tgatcaagga caaaaagccg ttcgactacg acacggaagt cgacggcatg
     2161 cgtgcgattg accgatacac ggtgcgtttc cggttcagcg aaccgcagcc gcgcttcatc
     2221 cagacgatga ccgggggcga cctgtgggga gccgtcgcgc gcgaggtcgt ggaggcctat
     2281 agcgacgaca tcgcggctca cccgatcggt actggcccgt tccgacttgc ggagtggcgg
     2341 cgctcgtcgc gcatcgtgct cgagcgcaat cccacatacc gcgaggtctt ctacgacgcc
     2401 gagccgaatg cggacgatgc ggctggccag gcactgctgg cgaagtacaa aggccacaag
     2461 ctgcccatcc tcgaccgcgt cgagatctcg gtgatcgaag agcagcagcc acgctggctt
     2521 tcattcctgc agaagcagca ggacctcgcc tggttgctgc caaacgagtt cgtcaacata
     2581 gcgataccca acaatcagct ggcaccgaac ctggcgaagc aaggcatgca gatgtaccgc
     2641 gtgctggcct cggacgtcac cctgaccgtc tacaacatgg atagccccgt gattggcggg
     2701 tacacgcccg accgtgttgc actccggcgc gcgctgagcc tgtcgctcaa caccgagcag
     2761 gaaatccgcc tggagcgcaa gaaccaggcg atacccgcgc aatcacctct cgttccgaac
     2821 acgatgggat tcgatccgaa ctacaagtcg gagggctaca acccgcaaaa ggccaaggcc
     2881 ctgctcgatc tttacggata tgtcgatcgc gacggcgacg gtttcagaga gcagccggac
     2941 ggctcaccgc tcgtcatcga gatttcgacg cagccggacc aggcgtcgcg tcgactcgac
     3001 gagctgcagc gcaaggactt cacggcggtt ggcatcaaga ccgagttcaa gcaggcgaag
     3061 tggccggaga accttcgcag cgtccgtgcc ggcaagtaca tggtctggcg cgtcggttcg
     3121 tcagccgcat cgcccgacgg ccagccggcg ctcgaccggt cggcatcgat ccacaagggc
     3181 ggtcagaacc tcgcgcgctt cagcaacaag cagttcgacg agatctaccg gcgcctgggc
     3241 gtgattccgg acggcccgga gcgggaccag ttgtttcgcg aggccaagcg cgtcgtcgcg
     3301 gcgtatgcgc cgtatcacaa cggagtgcac cggatcctga ctgatctggc gtggccctgg
     3361 ctgatcggct ttcggcggcc gccgtactgg cttgcctggt ggcagttcgt ggatgtcgat
     3421 gccgagcaa
//
LOCUS       DATIRT010000373         2636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_652281, whole genome shotgun sequence.
ACCESSION   DATIRT010000373 DATIRT010000000
VERSION     DATIRT010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652281"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VLN25_08420"
     CDS             complement(<1..215)
                     /locus_tag="VLN25_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase"
                     /protein_id="HSQ10626.1"
                     /translation="MLLLARQILRTGICTEPMPEPNDAWRVDGEQIQADLLALLGRAL
                     TIRQVDAGSCNGCELEINALNNPYYNL"
     gene            complement(215..1771)
                     /locus_tag="VLN25_08425"
     CDS             complement(215..1771)
                     /locus_tag="VLN25_08425"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HSQ10627.1"
                     /translation="MFRLQTETGELSFAQLGGELAVGWASADPAQWRAAAQALAREGA
                     TLVSLWGSDRRDLGDGLVVMAAYSRGHELLCLRLSMPASPYEFPSITAEFPAANRMER
                     TVFDLLGARALGDDDRPWLRHGAWPADYFPLRRDVDGAWSFEPTQDHYAFIPVTGDGV
                     HEIPVGPVHAGIIEPGHFRFSVVGEKVLRLEERLGYVHKGIDKRFEGMPLDQAVRLAG
                     RVSGDSTVAYAWAYSMAAESAAGAIVPARAVWLRALLLERERVGNHLGDLGALGNDAA
                     LAAGLAQFSRLRELWLRCNDEVFGHRLLMDCIVPGGVRIDPDASQFGRLHRQCSELAA
                     EVMTLRTIYEEHPGLQDRFVSTGVVTPELAAQLGLRGLAGRASGQVRDLRVDLRCVPY
                     DHLDVRIASDDRGDVAARVAVRFDELFESLRLIDAIMQRLPAGEVISPLPAAAHDAVI
                     GVGWVEGWRGDVLVALEVDPNGLLRRCHCHDPSWQNWPVLEHAVIGNIVPDFPLINKS
                     FNLSYSGVDR"
     gene            complement(1775..>2636)
                     /locus_tag="VLN25_08430"
     CDS             complement(1775..>2636)
                     /locus_tag="VLN25_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HSQ10628.1"
                     /translation="AAKGQLEPTVISLAFVFLLVGYGTKVGLAPLHNWLPDAHAEGPT
                     PISAVLSGLLLNVALYAVLRCKVLADGALGTRFAGDLMMGFGLLSVVLAAFFISRQRD
                     IKRMFAYSSIEHMGLITFAFGMGGPIASFAGLLHMTVHSLVKSAIFFTVGHAAQKAGT
                     QMMDQIRGLIRVNPTVGWGLMIGSLAILGMPPFGVFASEFLILTTAMREFPWTTPFLL
                     LALGVAFAAVLSKVQPMVFGETTVKPLAHPPALIPVFVHLALALLLGLYIPPYLEGWY
                     QQAARLVAGS"
BASE COUNT          505 a          871 c          857 g          403 t
ORIGIN      
        1 tcgaggttgt agtagggatt gttcagcgca ttgatttcga gttcgcagcc gttgcacgaa
       61 ccggcatcga cctgccggat cgtcagcgcg cgaccgagca gcgcgaggag gtcagcctgg
      121 atctgctcgc cgtccacgcg ccacgcgtcg ttgggttcgg gcatcggctc cgtgcagatg
      181 ccggtcctga ggatctgtct ggccagcaat agcatcagcg gtccactccg gaatagctca
      241 ggttgaacga cttgttgatc agcgggaagt cgggaacgat gttgccgatc accgcatgtt
      301 cgagcaccgg ccagttctgc cacgaggggt cgtggcaatg gcagcgccgc agcaggccat
      361 tgggatccac ttcgagcgcg accaggacgt cgccgcgcca gccttcgacc cagccgacgc
      421 ctatcactgc atcgtgtgcc gcggccggca gcggactgat gacctcacct gccggaaggc
      481 gctgcatgat cgcatcgatc agtcgcaatg attcgaacag ttcgtcgaac cgcacggcca
      541 cacgggccgc gacgtcgccg cgatcgtccg acgcgattcg aacgtccaga tggtcgtagg
      601 gtacgcatcg aaggtccacg cgcaggtcgc gcacctgtcc gctggctcgc ccggcgaggc
      661 cgcgtagtcc gagctgggcg gcaagctccg gggtcaccac cccggtgctg acgaagcgat
      721 cctgcagtcc ggggtgttcc tcgtagatcg tgcgcagtgt catcacttcg gcggcgagtt
      781 cgctgcactg gcgatggagc cgtccaaact ggctggcgtc cgggtcaatc cttacaccgc
      841 ctggaacgat gcaatccatc agcaagcggt gtccgaacac ctcgtcgttg cacctcagcc
      901 agagttcccg caaccgggag aactgagcca gccccgctgc cagcgccgcg tcgttgccga
      961 gcgcaccgag gtctccgagg tggttgccga cgcgttcgcg ctccagcagc agggcgcgca
     1021 gccacacggc gcgcgccggc acgatcgcac ccgctgccga ctcggcggcc atcgaatagg
     1081 cccacgcgta ggcgaccgtc gagtcacccg acacacgacc ggcgaggcgg accgcctggt
     1141 ccagtggcat gccctcgaag cgcttgtcga tgcccttgtg gacgtagccc agtcgctctt
     1201 cgagccgcaa cactttctcg ccgaccaccg agaaacggaa gtggcccggc tcgatgatgc
     1261 cggcatgcac cggaccgacc gggatctcat gcacgccgtc gccggtgacc ggaataaagg
     1321 cgtagtgatc ctgagtcggt tcgaatgacc atgcaccgtc aacatcgcgg cgcagcggga
     1381 agtagtccgc gggccaggcg ccgtgacgca gccacggacg atcgtcgtcg cccaaggcgc
     1441 gcgctccgag caggtcgaag accgtgcgct ccattcggtt ggcagccgga aactcggcag
     1501 tgatcgacgg gaactcgtac gggctcgccg gcatggagag acgcaggcac agcaactcgt
     1561 gtcctcgtga gtaggccgcc atgaccacca gtccatcgcc caggtcgcgc cggtcggagc
     1621 cccacagcga aacgagcgtg gcgccctcac gcgcaagtgc ctgggccgcc gcgcgccatt
     1681 gagcgggatc cgcgctcgcc cagccgaccg ccaactcacc gcccagttgc gcgaaactga
     1741 gttctccggt ttcggtctgc agtctgaaca tcggttagct tccggcgacc aggcgtgcgg
     1801 cctgctgata ccagccttcg aggtacggcg gtatgtacag gcccagcaac agcgcaaggg
     1861 cgaggtgcac gaagacgggg atcagcgccg gcggatgcgc cagtggcttc accgtcgttt
     1921 ccccgaacac catcggctgc accttggaca gcaccgcagc gaaagcgaca ccgagtgcca
     1981 gcagcaggaa cggcgtggtc caagggaact cgcgcatcgc cgtggtcagg atcaggaact
     2041 cgctggcgaa caccccgaaa ggtggcatgc cgaggatcgc gagcgagccg atcatcaggc
     2101 cccagccgac cgtgggattc acgcggatca ggccgcggat ctggtccatc atctgcgtgc
     2161 ccgccttttg cgcggcgtgg ccgaccgtga agaagatcgc tgatttcacc agcgagtgca
     2221 cggtcatgtg cagcaacccg gcaaaactgg cgatcgggcc acccatgccg aatgcgaagg
     2281 tgatgagtcc catgtgctcg atcgatgagt acgcgaacat gcgcttgatg tcgcgctggc
     2341 gcgagatgaa aaacgccgcc agcaccaccg agagcaggcc gaagcccatc atcaggtcgc
     2401 cagcgaagcg ggtgccgagc gcgccatcgg ccaggacctt gcaacggagc acggcataaa
     2461 gcgcgacgtt cagcagcagg cccgagagca ccgcagagat cggcgtcgga ccttcggcgt
     2521 gcgcgtcggg caaccagttg tgcaaagggg ccaggccgac cttggtgcca tagccaacca
     2581 gcagaaagac aaaggccagc gagatcacgg tgggctcgag ttgccccttg gccgca
//
LOCUS       DATIRT010000374         4075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_456396, whole genome shotgun sequence.
ACCESSION   DATIRT010000374 DATIRT010000000
VERSION     DATIRT010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456396"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VLN25_08435"
     CDS             complement(<1..367)
                     /locus_tag="VLN25_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSQ10629.1"
                     /translation="MDMFKTPGAFSWSELMTTDPAAAKSFYASLFGWSTKDMQMASGA
                     YTTCQVGEESVAGIMQIPTEAAGMPPMWGVYVTVADVDETIRQAEKLGGAVVVPPMDV
                     DGVGRMAVLRDPQGASFSVI"
     gene            611..2674
                     /gene="fusA"
                     /locus_tag="VLN25_08440"
     CDS             611..2674
                     /gene="fusA"
                     /locus_tag="VLN25_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010812847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HSQ10630.1"
                     /translation="MPYTTENLRTVALLGHGSAGKTTLAEALLARTGAIPAAGSVERG
                     TTVSDFDGLEKTFLHSLRTSVLHLDLPRTRVHLLDTPGFPDFIGQSIGALDAVETAAI
                     VVNAQSGIEMITSRMMDWAGRRKLCRVLIVNKIDAENVNLPQLLSDLRATFGKEVLPI
                     NLPSGGNTRVVDCFFNPSGDSDFSSVAEAHQALIDQVIEVDEDLMAKYLEQGEAIEPE
                     QLHAPFEKALREGHLIPVCFVSARNGAGIDELLKIFEALLPNPTEGNPPLFFKGEMID
                     GQPVEEFRSEPDPKKHVLAHVFKVVVDPYVGKLGVFRVHQGTLTKDTQLFIGTGRKPF
                     KVGHLFMLQGGKTVEMPSAVPGDIAAVAKVDEIDFDSVLHDSHDEDHIHMRPLEFPTP
                     MSGVAIAPKRRGDEQRISEVLHRMTAEDPTLKVEHDATLNETVLRGLGELHLRSALER
                     MAQQYKVEVDTRPPRVPYRETIAANAEGHARHKKQTGGAGQFGEVYLRVEPLPRGAGF
                     EFVDQVKGGVIPYNFIPAVQKGVESVLVSGPVAGFPMQDVRVIVYDGKHHPVDSKEVA
                     FVAAGKKAFLDALGKARPIVLEPIVNLEVEAPESHMGDITGDLSSRRGQVTGTRAMGP
                     SVLVVEGQAPLGELEGYSNRLKAMTQGRGAWTMTLSHYEQAPPPLQQQLANDYAAKRK
                     QEEDE"
     gene            complement(2707..>4075)
                     /gene="mnmC"
                     /locus_tag="VLN25_08445"
     CDS             complement(2707..>4075)
                     /gene="mnmC"
                     /locus_tag="VLN25_08445"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR03197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HSQ10631.1"
                     /translation="AALEAAGFDLHVRPGYASKREMLCGRYAPRWRVRRHEPPMAYGG
                     VRSALVIGAGLAGSACAHALARRGWSVQVIERAAAPARGASALPWGLLHPQMAADDAP
                     LARLTRAGVAATHAALARSVPAGDIDGRPIARCSGVFQMADGDASWARWQSAIARLGW
                     PPDYVEVLDQATAAQGVGMVPGHGGLWWPQGALVSPARWCSALLADKADLFNGDVVQL
                     KAGREGWTALDWSGHVLAAAPVVIVAAAIASPALLGMTRARVQTIRGRIAFLDPAPFA
                     NLQAPITGDGYLLRDPDGGVAAGATYELTLPNDAPEGSLSEARALQSNLVRLQRLLAS
                     PPPVDAIGSFDGLRSVARDRLPLAGAVPDELVAQAAARELRGAHLVDVPRRPGLYTCF
                     ALGSRGLTLAPLLAELIAASIEGEPLPIERALAAAVDPARFLLGSLRRDAALQSVRCD
                     PVPPP"
BASE COUNT          744 a         1359 c         1367 g          605 t
ORIGIN      
        1 tgatcaccga aaagctggcg ccctgcggat cgcgcagcac cgccatgcgg ccgacaccgt
       61 cgacatccat aggcggtacg acgacggcgc ccccgagctt ttcagcctgc cgtatcgttt
      121 cgtcgacatc ggcaacggtc acgtagacgc cccacatcgg cggcatgccg gcggcttccg
      181 tcgggatctg catgatgcct gccaccgact cctcgcccac ctgacaggtg gtgtaggcgc
      241 cgctcgccat ctgcatgtcc ttcgtgctcc agccgaacag cgacgcatag aacgacttgg
      301 ccgcggccgg gtcggtggtc atcagttcgc tccagctgaa cgcgccggga gttttgaaca
      361 tgtccatcgg gtgctccttt ctcagttggg caggacggag ggcgattgtg tgcgctccgg
      421 ggccgatggt gcagccgctg tgtgacgaaa tgccacttcg acggagtcga ccgggcccac
      481 gggatcaggg acgagtgctg cttgtcgaac gtcaatccca tggcgcggcc cggtgccggg
      541 cggctgacag cgcgggcgcc ggagggtaac gtcgcccttc gcaccagaaa tacactgctg
      601 aggagaggca atgccctaca cgacggagaa cctgcggacc gtggccctgt tgggccacgg
      661 cagtgcaggc aagaccacgc tcgcagaagc gctgctcgcc cggaccgggg cgatacccgc
      721 ggcaggaagt gtcgaaaggg gaaccacggt cagcgacttc gacgggctcg aaaagacctt
      781 cctgcactcg ctgcgcacgt ccgtgctgca tcttgatctg ccacgcaccc gagtccacct
      841 gctcgacacg cccggttttc cggatttcat cggccagtcg atcggcgcgc tcgacgcggt
      901 cgagacggcg gcgatcgtgg tcaatgcgca gagcgggatc gagatgatca cctcgcgcat
      961 gatggactgg gccggacgcc ggaagctgtg ccgcgtgctg atcgtcaaca agatcgatgc
     1021 cgagaacgtg aacctgccgc agctgctgtc ggacctgcgc gcgacgttcg gcaaggaagt
     1081 gctgccgatc aacctgccgt cgggcggcaa tacccgtgtg gtcgactgct tcttcaaccc
     1141 gagtggcgac tcggatttct cgtcagtggc tgaggcgcac caggcgctga tagaccaggt
     1201 catcgaggtc gacgaggacc tgatggccaa gtacctcgaa cagggcgagg ccatcgagcc
     1261 cgagcagttg cacgcaccgt tcgagaaggc cctgcgcgag ggccacctga tcccggtgtg
     1321 cttcgtttcg gcacgcaacg gcgcaggcat cgacgagttg ctgaagatct tcgaagcgtt
     1381 gttgccgaat ccgaccgaag gcaatccgcc gctgttcttc aagggcgaga tgatcgacgg
     1441 acaaccggtc gaggaattcc gctcggaacc cgacccgaag aagcatgtgc tggcgcatgt
     1501 gttcaaggtc gtcgtcgatc cgtacgtcgg caaactcggc gtcttccgcg tccatcaggg
     1561 gacgcttacc aaggacacgc agcttttcat cggcactggc cgcaagccct tcaaggtcgg
     1621 gcatctgttc atgctccagg gaggaaagac cgtcgagatg cccagcgcgg tgccgggcga
     1681 catcgccgcg gtcgccaagg tcgacgagat cgacttcgac agcgttctgc atgactcgca
     1741 tgacgaggat cacatccaca tgcggccgct cgagttcccg acgccgatga gcggtgtggc
     1801 gatcgcaccg aagcgccgcg gcgacgagca gcgcatctca gaagtgctgc accggatgac
     1861 ggccgaggat ccgacgttga aggtcgaaca tgacgcgaca ctgaacgaaa cggtgctgcg
     1921 cggcctgggt gaactgcacc tgcgctcggc gctggaacgg atggcgcagc agtacaaggt
     1981 ggaggtcgac acacggccgc cgcgcgtgcc ttatcgcgag accatcgcgg caaacgccga
     2041 gggccacgcg cggcacaaga agcagaccgg aggtgccggc cagttcgggg aggtctacct
     2101 gcgcgtcgaa ccgctgccgc gcggcgccgg attcgagttc gtcgaccagg tcaagggcgg
     2161 cgtcattccg tacaacttca ttccggcggt gcagaagggc gtcgagtcgg tgctggtgtc
     2221 aggcccggtc gccggtttcc cgatgcagga tgtgcgcgtc atcgtctatg acggcaagca
     2281 ccacccggtg gactccaagg aggttgcgtt cgtcgcggcg gggaagaagg ccttcctcga
     2341 tgcgctcgga aaggcccgtc cgatcgtgct cgagccgatc gtcaatctcg aggtggaggc
     2401 cccggaatca cacatgggcg acatcaccgg ggatctttca tcgcgtcgcg gccaggtgac
     2461 cggcacacga gcgatggggc cttcggtgct ggtcgtcgaa ggccaggcac cgctgggcga
     2521 gctcgaaggc tattcgaacc gcctcaaggc catgacgcag ggtcgcggcg cgtggacgat
     2581 gacgttgtcg cactacgagc aggcaccgcc tcctctgcaa cagcagctcg ccaacgacta
     2641 cgcggccaag cgcaagcagg aagaagacga gtaagcgcgc aggagcacgg cttgtgccac
     2701 ctccgcctag ggcggcggca cgggatcgca gcggacgctc tgcaaggcgg cgtcccgacg
     2761 caatgagccc agcaggaagc gtgcgggatc gaccgcggcg gcgagcgcac gctcgatcgg
     2821 caacggttcg ccttcgatcg acgccgcgat cagttcggcg agaagcggcg cgagcgtcag
     2881 gccacgcgag ccgagggcga agcacgtgta caggccgggc cgccgcggaa catcaaccag
     2941 atgtgctccg cgaagttcgc gtgcggccgc ctgcgccacg agttcatccg gcacggcacc
     3001 cgccagcggc aaccgatcac gcgccacgga gcgcaggcca tcaaaggagc cgattgcatc
     3061 gaccggggga ggcgaggcga gcaggcgctg caaccgcacg aggttgctct gcagcgcacg
     3121 ggcctcgctc agcgaaccct cgggtgcatc gttaggcagc gtgagttcat aggtcgcacc
     3181 ggcggccacg ccgccgtccg gatcacgcag cagatagccg tcgccggtga tgggcgcctg
     3241 cagattcgcg aagggtgcag gatcgagaaa ggcaatgcgg ccacggatgg tctgcactct
     3301 cgcccgcgtc atcccgagca acgcaggcga tgcaatcgcg gcggcaacga tgaccaccgg
     3361 cgcagctgcg agcacatgac ctgaccagtc cagtgcggtc cagccttccc ggcctgcttt
     3421 cagttgaacg acgtcgccgt tgaacaggtc cgccttgtcc gccagcaacg cggagcacca
     3481 gcgtgcaggt gacaccagcg ctccctgcgg ccaccagagc ccgccatgcc ccgggaccat
     3541 gccgacccct tgcgccgccg tcgcctgatc cagcacctcg acatagtccg ggggccagcc
     3601 gagtcgcgcg atcgcggact gccatcgggc ccacgacgcg tccccgtcgg ccatctggaa
     3661 caccccgctg cagcgggcga tcggccggcc atcgatgtcg cccgccggca cgctccgcgc
     3721 aagcgccgcg tgcgtcgcgg cgacgccggc gcgtgtcagg cgcgcgagcg gtgcatcgtc
     3781 ggccgccatc tgtgggtgca gcagccccca gggcaaggcc gacgcgcccc tcgccggtgc
     3841 cgcggcgcgt tcgatcacct gcacggacca gccacgccgc gccagcgcat gcgcgcaggc
     3901 actgccggcc agcccggcgc cgatcacgag ggccgaacgc actcccccgt aagccatcgg
     3961 aggctcatgg cggcggacac gccagcgcgg cgcgtagcgg ccgcacagca tttcgcgctt
     4021 gctggcgtag cccggccgca cgtgcagatc gaagcccgca gcttccagag ccgca
//
LOCUS       DATIRT010000375         2375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1145202, whole genome shotgun sequence.
ACCESSION   DATIRT010000375 DATIRT010000000
VERSION     DATIRT010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145202"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..152)
                     /locus_tag="VLN25_08450"
     CDS             complement(<1..152)
                     /locus_tag="VLN25_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSQ10632.1"
                     /translation="MAQVAIRGVAKRFGGTTVIHGVDIDIADGEFAVLVGPSGCGKTT
                     LLRMIAG"
     gene            complement(196..1164)
                     /locus_tag="VLN25_08455"
     CDS             complement(196..1164)
                     /locus_tag="VLN25_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HSQ10633.1"
                     /translation="MKALITDFDFPDIALERGLFAEAGITLSDAQCRTEDELIAAARE
                     CDALLLQYAPVTDRVLSALPRIGLVSRYGAGFDTVDTVACLRHGVWVANSPDYGVGEV
                     ATHALAMTLALLRHLAIYDRDVKAGRWSYLSPGTIRRASDLTVGILGLGRIGKRYAHL
                     ARNCFARVIACDPYIIDGDFPAYVERVTLEALFEHSDIVSLHVPLTDETRGMVATPLL
                     ARMRRGSLLVNTARGAVVDVDAVLAQLDAGTLDGAALDVLPAEPVATSHPLARHPRVL
                     LTPHAAFYSTAAEVELRRKAAMNIVQWAKTGRPMYPVIQGTKIPAG"
     gene            complement(1161..1745)
                     /locus_tag="VLN25_08460"
     CDS             complement(1161..1745)
                     /locus_tag="VLN25_08460"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HSQ10634.1"
                     /translation="MSRSAPTASRKRCGWAGDDPLMVAYHDDEWGMPSHDDRHLFEML
                     ILEGAQAGLSWTTILRKREGYREAFDDFDVDRVARYTLARIERLLKNPAIVRNRAKVE
                     SAVLNARAVRAIRDESGSLDRYLWTFSGGRPIVNRWKDYREAPATSAESDAMSKALKS
                     RGCKFVGSTICYAFMQATGMVDDHQRDCWRRAPA"
     gene            complement(1769..>2375)
                     /locus_tag="VLN25_08465"
     CDS             complement(1769..>2375)
                     /locus_tag="VLN25_08465"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HSQ10635.1"
                     /translation="KAGIAVLRGNLAPDGAVIKPSAATPALMQHRGRAVVFENSADMH
                     AKVEDESLDVRSDDILVLKNCGPRGYPGMAEVGNMPIPAKLVKQGVTDMVRISDARMS
                     GTAYGTVVLHAAPEAAAGGTLALVKSGDFIELDVEHRRLHLEVTDAELARRRAAWKAP
                     AAPMPRGYTKLYFDHVQQADKGADLDFLVGSSGSAVPKDNH"
BASE COUNT          388 a          812 c          769 g          406 t
ORIGIN      
        1 ccggcgatca tgcgcagcag cgtggtcttg ccgcagccgg agggacccac caggaccgcg
       61 aactcgccgt cggcgatgtc aatgtcgacg ccgtggatca cggtcgtccc accgaatcgc
      121 ttggcaacgc cgcgaatcgc gacttgcgcc ataaagcgtt gtctcctccg cgcggcaggg
      181 aaggtgccgc tgcgatcaac ctgccgggat cttcgtccct tgaatcacag gatacatggg
      241 gcgtcctgtc ttcgcccatt gcacgatatt catggccgcc ttgcgccgca gctcgacttc
      301 cgccgcagtc gaataaaacg ccgcgtgcgg cgtcagcagc acgcgcgggt ggcgcgcaag
      361 cggatggctg gtcgctaccg gttccgccgg caatacgtcg agcgctgcac cgtcgagcgt
      421 tccggcgtcg agctgcgcaa gcacggcgtc gacatcgacc accgcgcctc gtgcggtgtt
      481 caccagcagg ctgccgcggc gcatgcgcgc cagcagcggc gtcgcaacca tgccgcgcgt
      541 ctcgtcggtc agtggcacat gcagcgagac gatgtcgctg tgctcgaaca gcgcctccag
      601 cgtgacacgt tctacatacg cgggaaaatc gccgtcaatg atgtagggat cgcaggcaat
      661 gacgcgcgcg aagcaattgc gggccaggtg ggcatagcgc ttaccgatgc ggccgagccc
      721 gaggatgccg accgtcaggt cgctggcgcg gcggatcgtg cccggcgaca ggtagctcca
      781 gcggccggcc ttgacgtcgc ggtcgtagat cgccagatgc cgcagcagcg cgagggtcat
      841 cgccagcgcg tgcgtggcca cttcgccgac gccgtagtcg ggtgagttcg cgacccagac
      901 accgtggcgc aggcaggcca ctgtgtcgac cgtgtcaaag ccggcgccgt aacggctgac
      961 gaggccgatg cgcggcaagg cactcagcac acggtccgtg accggggcgt actgcagcag
     1021 cagcgcatca cactcccgcg ccgccgcgat cagttcgtcc tcggtgcggc actgcgcgtc
     1081 ggaaagcgtg atgccggcct cggcaaacag cccccgctcg agcgcgatgt ccgggaagtc
     1141 gaagtccgtg atcagcgcct tcatgccggt gcccgccgcc agcagtcgcg ctggtgatca
     1201 tcgaccatgc cggtcgcctg catgaacgcg taacagatgg tcgagccgac gaacttgcag
     1261 ccacgcgact tcagtgcctt gctcattgcg tcggactcgg cgctggtggc cggtgcctcg
     1321 cgatagtctt tccaccgatt cacgatcggt cgcccgccgc tgaatgtcca cagatagcgg
     1381 tccaacgagc ccgattcgtc gcggatcgcc cgcaccgctc gcgcgttcaa cactgctgac
     1441 tcgaccttgg cgcggttgcg cacgatggcc ggattcttta gcaggcgctc gatgcgagcc
     1501 agcgtgtaac gcgcaacgcg gtcgacatcg aagtcgtcga aggcttcgcg gtaaccctcg
     1561 cgcttgcgca ggatcgtcgt ccacgacagg ccggcctgcg cgccctcgag gatcagcatt
     1621 tcgaaaaggt gccgatcatc gtgcgaaggc ataccccatt catcgtcgtg gtacgcgacc
     1681 atcagcggat cgtcgccggc ccagccgcag cgcttcctgc tcgccgtcgg cgcgctcctg
     1741 ctcatggaag acctggacct tggcacggtc agtggttgtc tttggggact gcggagccac
     1801 tggacccgac caggaagtcg aggtcggcgc ccttgtctgc ctgctgcacg tgatcgaagt
     1861 agagcttggt gtagccgcgc ggcatcggtg cggccggcgc cttccaggcc gcgcgccggc
     1921 gtgcaagttc ggcgtcggtg acttccagat gcaggcgccg gtgctcgaca tccagttcga
     1981 tgaagtcgcc acttttcacc aatgcgagcg tgccgcccgc cgcggcttcc ggcgcggcat
     2041 gcagcacgac cgtgccatac gccgtgccgc tcatgcgcgc gtcagagatg cgcaccatgt
     2101 cggtgacacc ctgcttcacg agcttggccg gaatcggcat gttgccgacc tcggccatgc
     2161 ccggataccc cctgggtccg cagttcttca gcaccaggat gtcgtcggac cgcacgtcga
     2221 gcgactcgtc ctcaaccttg gcgtgcatgt ctgcgctgtt ctcgaacacc accgcgcggc
     2281 cgcggtgctg catcaatgcc ggtgtcgccg ccgacggctt gatcaccgca ccatcgggcg
     2341 cgaggttgcc acgcagcacc gcgatgccgg ccttg
//
LOCUS       DATIRT010000376         4925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1243376, whole genome shotgun sequence.
ACCESSION   DATIRT010000376 DATIRT010000000
VERSION     DATIRT010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243376"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="VLN25_08470"
     CDS             <1..923
                     /locus_tag="VLN25_08470"
                     /inference="COORDINATES: protein motif:HMM:NF015825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HSQ10636.1"
                     /translation="QGGTLPVRTELQWVQSIQKAAVRVNYSGTVVYQSGGEMSSSRIK
                     HLFDGANSHERVQTLDGKPREYIRVRTGNNDEVHCLIPESRRVVIEHRAVEDPFPGLT
                     GAPAEAILERYDVKIGGIERVAGIECQVLTLEPRDALRYGYRLCVDRATGLLLKAQTL
                     GEGRDVVEQVAFTDIRIGERIDRSRLKPAWPTEGWTVERTEYRRVDLEKAGWLVPTPE
                     GFRRTKEVLRRMGPRDAMQVVFSDGLATISVFIEPAADTKVAGDAVQVMGPTAAYSRR
                     LGDALVTVVGEVPPEAVRTVSKSVEFRGPR"
     gene            969..2429
                     /locus_tag="VLN25_08475"
                     /pseudo
     CDS             969..2429
                     /locus_tag="VLN25_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559741.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
     gene            2605..4398
                     /gene="lepA"
                     /locus_tag="VLN25_08480"
     CDS             2605..4398
                     /gene="lepA"
                     /locus_tag="VLN25_08480"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HSQ10637.1"
                     /translation="MQHIRNFSIIAHIDHGKSTLADRLIQRCGGLSDREMEAQVLDSM
                     DLERERGITIKAQTAALEYVAADGRKYNLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIELGVEVLPILNKMDLASANAEEARAEIEDVIGLDAHDAVL
                     ASAKTGMGLEEILERIIERVPPPKGDTAASLQALIIDSWFDNYVGVVMLVRVVNGVIK
                     PKDKILLMASGATHLVEQVGVFTPKSRSRDSLSAGEVGFVISGIKELRDAHVGDTVTH
                     AGKPAAQPLPGFKEVKSQVFAGLFPVESNQYEALRDALTKLQLNDASLHFEPEVSQAL
                     GFGFRCGFLGLLHMDIVQERLEREYDMDLITTAPSVVYEVLLRDGSVLSVENPSKMPD
                     PARIEEIREPVVDVTIFVPQEFVGQVITLCIGKRGIQTDLTYHSRHVILKYELPLAEI
                     VMDFFDKLKSVSRGYASMDYEFKEYRAADVVKVDMLINGDRVDALSVIVHRANATYRG
                     REVTAKMRKLIPRQMYDVAIQASIGANIIAREDVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKKRMKQVGTVEIPQEAFLAILQVEDK"
     gene            4395..>4925
                     /gene="lepB"
                     /locus_tag="VLN25_08485"
     CDS             4395..>4925
                     /gene="lepB"
                     /locus_tag="VLN25_08485"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HSQ10638.1"
                     /translation="MNFALILFVLTIFTGILWVADKMVFAKQRRARAAKLLADFDSRN
                     AAALARKEAIVEQERRSLEDGAVRQPAWLEYTASFFPVILLVFLLRSFLYEPFRIPSG
                     SMLPTLEVGDLILVNKYEYGIRLPVINKKVIDVGQPKRGDVMVFRFPLNPSQDYIKRV
                     VGLPGDRIEYFNKRLTV"
BASE COUNT          926 a         1504 c         1621 g          874 t
ORIGIN      
        1 ggcagggcgg caccttgccg gtgcgcacag aactgcaatg ggtccagtcg attcagaagg
       61 ccgcggtgcg cgtgaactac agcggcactg tcgtctacca gtccggcggc gagatgagct
      121 cgtcgcgcat caagcacctg ttcgatggcg ccaattcgca tgagcgcgtg caaacgttgg
      181 acggcaagcc gcgtgagtac atccgggtgc gcaccggcaa caatgacgaa gtgcattgcc
      241 tgatccccga atcgcgccgc gttgtcattg agcaccgcgc cgtcgaggat ccgtttcctg
      301 ggctgaccgg cgctccggcc gaggcgatcc tggagcggta cgacgtgaag atcggcggca
      361 tcgagcgcgt tgcgggcatc gagtgccagg tactgacact cgagccgcga gacgcgctgc
      421 gctacggcta tcgtctgtgc gtggaccggg cgaccggctt gctgctgaaa gcgcagaccc
      481 tgggcgaagg aagggacgtg gtggaacagg tcgccttcac cgatattcgc atcggcgaac
      541 gcatcgatcg ctcgcgcctg aagccggcgt ggcccaccga agggtggacc gtcgaacgca
      601 cggaatatcg acgtgttgat ctcgagaagg cgggctggct ggtaccgacg cccgagggat
      661 ttcgccggac taaggaagta ctgcgccgga tgggcccacg cgacgcgatg caggtcgtct
      721 tcagcgatgg cctggccacc atttccgtat tcatcgagcc ggcggccgac accaaggttg
      781 ccggcgacgc agtccaggtc atggggccga ccgcagccta ttcgcgtcga ctcggcgatg
      841 cgcttgtcac cgtagtcggc gaagtcccgc cggaagccgt gcggactgtg tcgaagtcag
      901 tggagtttcg tggcccccgc tgaagtgcct gggcaggggt cgtctcaagt tcgtagagga
      961 agacaggaat gatcagaaaa tttactgctg cacttttcgc cccgctgctg gcggttgcat
     1021 taccgctgct cgcttccggc gctcttgcgc agccgagcca gggcctgccc gacttcgccg
     1081 atctggcgga gcgagcggga ccggcggtcg tgaacatccg tacgacggcc cgtttggctc
     1141 ccaatgcagg ggctctgccg ttcccggaca ttgacgagaa cgacccgatg tactagttct
     1201 tccgcctctt cttccccaat cccccgcaac agggaccgca gccgggcccg cgtcaggggc
     1261 cgggccaggc tcctggcccc cgccgtgatg ttccgcgtgg cctgggatcg ggtttcatca
     1321 tctcggccga cgggtatgta atgacgaacg cacatgtcgt cgagggcgct gacgacatca
     1381 cggtgacgct gaccgacagg cgcgaattca agggcaaggt catcggcgtc gaccgccgta
     1441 ccgatgtcgc gctggtcaag atcgaggcgg ccggactgcc gaatgtcaag gtgggggatc
     1501 cgaaccgctt gcgtgtgggg gagtgggtcg tggcgatcgg ttcgccattc ggcttggaaa
     1561 acaccgtcac cgccggcatc gtcagcgcga aagcgcgcga gaccggtgag ttcctgccct
     1621 tcatccagac cgatgtggcc gtgaatccgg gcaactcggg cgggccgctg ctcaacatgc
     1681 gcggcgaagt cgtcggcatc aactcgcaga tcttcaccac gtccggcggc tacaacggca
     1741 tttcctttgc gattccgatc gacgaggcga tgaacgtgca gcagcagctg cgctcgaccg
     1801 gtcgcgtgat ccgcgggcgg atcggagtcg gaatcgaggg tgtctccaag gaggtcgccg
     1861 aagcgatcgg tctcggggca ccgcctcgtg gcgcgctggt caattccgtc gagaaggacg
     1921 gtccggcagc caagtcgggg ctggaaccgg gcgacgtcat tctgcgtttt gacggacgag
     1981 cgatcgagcg ctcgagcgat ctcccgcgca ttgtcggcgg aaccaagccc ggtaccaact
     2041 ccatgctgac ggtgtttcgc aaaggttcgc agcgcgaact gaaggtcaca gttggcgaga
     2101 tgccgcagga tcggccgcgc agcgcccgcg ccgcagagcc ggccaagccg gcgacacctg
     2161 ccaacgctct gggcctcggc gtgtccgatg tgacgtcgga caagctcaag gagctcggca
     2221 tcaaaggcgg cgtccaggtc gatgtagtcg atggcctggc cgccgcggcg ggactgcggg
     2281 ccggtgatgt catcacacag atgaacagtg gcgaagtgca gaacgccaag cagttcaatg
     2341 aagccgtggc aaagctcgat ccgaagaagg acgttgctgt gctcgtgcgg cgtggcgaag
     2401 cctcgcgctt cgtgatcatc cggcgctgat ccggcgcggg tccgcggcct ttggccgggt
     2461 cgcggtaaaa tccgcggccc taccggtagt gggggtgtgc cttgcggtca cccccatttc
     2521 tttgttgtgt ggcagcccga cttgccgccg acgccacgat tcgcattgcc ggggaccatt
     2581 gccggtcgtt ccggcgcaaa gcgcatgcag cacatccgta atttttccat catcgcgcac
     2641 atcgaccacg gcaagtcgac gcttgcggat cgcctgatcc agcggtgcgg cggcctgtcg
     2701 gatcgcgaga tggaggcgca ggtgctggac tcgatggacc tcgagcgcga gcgcggcatc
     2761 acgatcaagg cgcagaccgc cgcgcttgag tacgtcgctg ccgacggtcg gaaatacaac
     2821 ctcaacctga tcgacacgcc gggccacgtg gatttcagct acgaagtcag ccgctcgctg
     2881 tctgcgtgcg aaggcgcgtt gctcgtggtc gatgcttcgc agggagtcga ggcccagacc
     2941 gtcgccaatt gctacaccgc gatcgaactc ggtgtcgaag ttctgccgat tctcaacaag
     3001 atggacctgg cctcggccaa tgccgaggag gcgcgcgcag agatcgagga tgtgatcggg
     3061 ctcgatgccc acgatgcggt cctggccagt gccaagaccg gcatgggcct cgaggagatt
     3121 ctggaacgga tcatcgagcg cgtgcctccg cccaaagggg acacggcagc ctccctgcag
     3181 gctttgatca tcgactcatg gttcgacaac tacgtcggtg tcgtgatgct ggtgcgcgtc
     3241 gtcaacggcg tgatcaaacc gaaagacaag atcctgctga tggccagcgg cgccacacac
     3301 ctagtcgagc aggtcggcgt gttcacgccc aagtcgcgca gccgggactc tctgtcggcg
     3361 ggcgaggtcg gatttgtcat ctcgggcatc aaggaactgc gcgacgccca tgtgggcgac
     3421 acggtcaccc atgcggggaa gccagcggcc cagccactgc ccggtttcaa ggaagtgaag
     3481 tcacaggtat tcgcgggact cttcccggtc gaatccaacc agtacgaagc gttgcgcgat
     3541 gcgctgacca agctgcagct gaacgacgcg tcactgcact tcgagcccga ggtgtcgcag
     3601 gcgttgggat tcggcttccg ctgcggtttc ctcgggctgc tgcacatgga catcgttcag
     3661 gagcggctcg agcgcgagta cgacatggac ctcatcacca ccgcaccgtc ggtggtctat
     3721 gaggtgctgc tgcgtgacgg cagcgtcttg tcggtcgaga acccatcgaa gatgcccgac
     3781 ccggcgagga tcgaggagat ccgcgaaccg gtcgtcgacg tcaccatttt cgtgccgcag
     3841 gagttcgtcg gacaggtgat cacgctgtgc atcggcaagc gcggcatcca gaccgacctg
     3901 acctatcaca gccgccatgt cattctgaag tacgaattgc cgctggcaga aatcgtgatg
     3961 gacttcttcg acaagctgaa gtcggtgtcg cgcggctatg cgtcgatgga ctacgagttc
     4021 aaggaatacc gcgcggccga cgtcgtcaag gtcgacatgc tgatcaacgg cgaccgggtc
     4081 gatgcgctga gcgtcatcgt tcaccgggcc aatgccacct accgcggtcg tgaagtgacc
     4141 gccaagatgc gcaagctgat cccgcgacag atgtacgacg tggcgatcca agcgtcgatc
     4201 ggggccaaca tcatcgcgcg cgaagatgta aaggcgctgc gcaagaacgt gctggccaag
     4261 tgctacggcg gcgacatcac gcgcaagaag aagctgctcg aaaagcagaa agccggcaag
     4321 aagcgcatga agcaggtcgg cacggtcgag attccgcagg aagccttcct cgcgatcctg
     4381 caagtggaag acaaatgaac ttcgcattga ttctgttcgt cctgacgatc ttcaccggca
     4441 tcctgtgggt cgccgacaag atggtgtttg ccaagcagcg gcgtgcccgc gcggcgaagc
     4501 tgctggccga cttcgacagc cgcaatgcgg ccgcgctggc acgcaaggag gccatcgtcg
     4561 aacaggagcg gcgcagcctg gaggacggtg cggtccggca gccggcctgg ctcgagtaca
     4621 ccgccagctt ctttccggtg atcctgctgg tgttcctgct gcgatcgttc ctctatgagc
     4681 cgttccggat tccgtcggga tcgatgctgc cgacactcga agtgggcgac ctcatcctgg
     4741 tcaacaagta cgaatacggc atccgcctgc cagtgatcaa caagaaggtg attgacgtcg
     4801 gccagccaaa gcggggcgac gtcatggtgt tccgcttccc gctgaaccct tcgcaggact
     4861 acatcaagcg cgtcgtcgga ttgccgggcg accgcatcga gtatttcaac aagcgcctga
     4921 cggtc
//
LOCUS       DATIRT010000377        10692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1145426, whole genome shotgun sequence.
ACCESSION   DATIRT010000377 DATIRT010000000
VERSION     DATIRT010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10692
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145426"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VLN25_08490"
     CDS             complement(<1..503)
                     /locus_tag="VLN25_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007742679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HSQ10639.1"
                     /translation="MNTTIRSIHARRVWDSRGRPTVEAEVTLNDGVMGRAIVPAGASK
                     GPREALELRDGTARFGGLDVLRAVANVNGEIARALVGHAADDQAALDARLIALDGTDA
                     KTRLGANATLAVSMAAAHAVAAAQGVPLYEYIGGPEATLLPMPQIQIFGGGAHAGRRV
                     DVQDFMIV"
     gene            complement(500..1516)
                     /locus_tag="VLN25_08495"
     CDS             complement(500..1516)
                     /locus_tag="VLN25_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HSQ10640.1"
                     /translation="MSAATETEVPAEFAQALRELGLADAGAIVGTPMTGGVSSEIWLI
                     QTAQGPVCAKRALAKLRVAADWRAPIARNRYEARWMRVASDAYAGCAPTVLGQHPSLG
                     VLVMDYLPPSAFSLWKHRLRDGHADPATAAEVGRVLGKIHSYSARRPELATEFDTDTI
                     FFDIRLEPYLLATAKKHPELASRLEHLVDVTRSNRVALVHGDVSPKNILIGASGPVFL
                     DAECAWWGDPAFDIAFCLNHLLLKCLWTPAATPAFLASYDGLAKAYLQSVDWEPSGVL
                     EQRAAALLPGLFLARIDGKSPVEYVTEERQREQVRRVARELLKHPVASLRSVRTRWAE
                     ELEQ"
     gene            complement(1500..2453)
                     /locus_tag="VLN25_08500"
                     /pseudo
     CDS             complement(1500..2453)
                     /locus_tag="VLN25_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912020.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
     gene            2670..3659
                     /locus_tag="VLN25_08505"
     CDS             2670..3659
                     /locus_tag="VLN25_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HSQ10641.1"
                     /translation="MLAAYYERVGAARDVLSVAEIDAPAAGPGEVRVKLTCSGVNPSD
                     VKSRGGVRSRELPFPRIVPHSDGAGVIDQVGAGVSGHHIGQRVWVWNAAWGRAFGTAA
                     QYVVLPEAQAVPLPDNIGFAEGACLGIPGLTAYHAVHSNGGVAGKSVLVAGGAGAVGH
                     YAVQFAKLAGARLIVATVSTAAKATLARAAGADMTLNYKSEDVGQRCVQLTDGNGIDR
                     IIELDLATNLATDLAAVRQDGEITVYGSTAPEIGVPFFASALKNVRYQFFIVYKLSLA
                     DRATAIDEVSALLDGNRIQHNIAARLPLTHIAEAHELVESGRATGNVVVDIPV"
     gene            complement(3688..4128)
                     /locus_tag="VLN25_08510"
     CDS             complement(3688..4128)
                     /locus_tag="VLN25_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSQ10642.1"
                     /translation="MKGIAQILRGKPSKVVHAVAPNDTVYDAVKKMAEQGIGALLVME
                     GERIVGIVSERDYARKIALAGRSSKETPVSVIMTTEVLCVGPLQTTEECMAIMTENRV
                     RHLPVIDNGSLIGLVSIGDLVKDIISEQQFIIEQLEHYISGSRG"
     gene            complement(4402..5526)
                     /locus_tag="VLN25_08515"
     CDS             complement(4402..5526)
                     /locus_tag="VLN25_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HSQ10643.1"
                     /translation="MRINGAVLHEMGALTPYSESRPLAIEQVELEPPGTGEVLVKIAA
                     AGLCHSDLSVINGDRPRPMPMLLGHEAAGIVEHAGTGVDDLRKGDHVVMVFVPSCGHC
                     GPCAEGRPALCEPGAVANTAGTLLTGARRLTRDGQPLNHHLGCSAFAEYATVSRRSLV
                     KIDPELPLAEAALFGCAVLTGVGAVVNTARVSVGQTVAVIGLGGVGLAALIGAVCAGA
                     SRIVAIDLDDDKLTLAASLGATDTYNAKSESCSEDVRTATRGGVDFALEMAGSVKAME
                     LAYRITRRGGTTISAGLPPPTHVFPVPHVNMVAEERTIKGSYIGTCVPVRDLPRYISL
                     YRQGRLPVDRLLTGRLRLADINLGFDRLREGTAVRQVVEF"
     gene            complement(5539..6201)
                     /locus_tag="VLN25_08520"
     CDS             complement(5539..6201)
                     /locus_tag="VLN25_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSQ10644.1"
                     /translation="MLRIDQLNVAIGSVAILRNANLAVEAGEFAGLIGRNGAGKTTLM
                     RSIMGILPAASGKITFEGQALNTLPVHRRAQLGIGYMPEDRRLIPELTVEENILLPAW
                     ATGVTNPSERLAFVYRVIPEAREFAARRGLQLSGGQQKLAALARALMCGTRLLLLDEP
                     FEGVAPVLCQRLVEVIGRLKKEGLSVILSESDLTHSSDMLDSVYLIDRGAVTRGELQQ
                     VD"
     gene            complement(6188..6976)
                     /locus_tag="VLN25_08525"
     CDS             complement(6188..6976)
                     /locus_tag="VLN25_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSQ10645.1"
                     /translation="MSAPIIETSGVNKNFGAVTAAAELTVSIDADTVVGLIGGNGAGK
                     TTFLNMLTGYMKPDRGSIRYAGHDIVKLVPRQVTRLGMCRSFQIPQLFASLTVRDNLL
                     VALGVVVLNASRWQGGEAIAADRIDAVANETLVRFGLQDWRDKPAAVLPEGVRKLLDI
                     AMAMVVQPKVLLLDEPTSGVSADEKFAIMDRVMDAVRAARVTIVFVEHDMDIVRRYAQ
                     RVLAFYEGRIIADGAPEQVLNDDEVRRYVVGTIHATSVASHAAH"
     gene            complement(6973..7923)
                     /locus_tag="VLN25_08530"
     CDS             complement(6973..7923)
                     /locus_tag="VLN25_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSQ10646.1"
                     /translation="MTRATIAIISGLILTLVVGWFMPGWVQSLLILALGKGLVVLGLL
                     LLMRCGLVSFGQGLFYALGAYAAVSITRIFGLPETLLTSDAVVMPLAGGVVAGILAFI
                     LGFLLAAYRSIFFAMLSLAFSMILYGLLIKSSVLGSSDGFNVAYATVAGIALPAGMHL
                     YGLFAVSAIFVAAALVATHAFVGSHTGRLAPAIRDNELRVEYMGASVRRIVHLNYVLA
                     ASLSGVGGGLVALSIGHIDPDLAYWTTSGEFVFVAILSGTGNVVAPFIGSLVFEWIKS
                     VAYQYTPDTWQLVLGTAMLLVIMFMPAGLWSLFVRRKKAA"
     gene            complement(7920..8816)
                     /locus_tag="VLN25_08535"
     CDS             complement(7920..8816)
                     /locus_tag="VLN25_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSQ10647.1"
                     /translation="MTGLMSVLVDGIVYSSYLFIIAVGLTLIYGVMRILNLAHGSLYA
                     LGAYVAATLVGAWFSAGLNPYGSFALLALAAIVVGLVAGPLIERGLLRFMYGKDEVVL
                     ILVTFSLFLILEDVIKLIWGVESYTIYQPYGLLGNVDLADLPYTVYDLLLVVVAIVVG
                     AALTFVLTRTRQGKLLLAVIHDREVSAAMGINVARMYLVTFTLGTMLAALGGALIAPK
                     ISIVPAMGVEVIVLAFAVVVIGGLGSLPGAALGALIVGIVRSAAVHYWPEVELFSIYL
                     VMALVLIARPRGLFSAAEARKI"
     gene            complement(8873..10189)
                     /locus_tag="VLN25_08540"
     CDS             complement(8873..10189)
                     /locus_tag="VLN25_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSQ10648.1"
                     /translation="MTEVRRQLTRAFVAAGLAMAAAALSAPVGAQDKAAPEKPIKIGI
                     VTFLSGAAAGPFGVPARNGAEVIVEALNGGKVPAPYGTAGLGGAPIEVVFVDEAGGTT
                     KQVNEYRNLVQRDNVDVVIGYISSGDCLAVAPVAEELKLLTVFFDCGTPRIFEDASYK
                     YLFRTGATATMDNTAAALYVTEMKPGLKTVAAINQNYAWGQDSWTDFEAAIKVLNPDA
                     KIATSQMPKLLAGQYGAEISALLTSGADVIHSSFWGGDAEALILQGAPRGLFKKSTIV
                     MTAGEPTLVRLGTQVPEGTVIGARGPFNVFAPDTELNRWFRKAYEERYAVAPSYPAYK
                     MVQAFLGVKAAWDKAQAANGGKRPNQDQVVAAFENLTFEGPGGSVSMSLGKGHQAVQG
                     TAYGTVKHDGSKVTVVNVKRYPASRITPPDGVKSEEWIKSGFKTAK"
BASE COUNT         1962 a         3543 c         3292 g         1895 t
ORIGIN      
        1 acgatcatga agtcctgcac gtcgacccgg cggcccgcgt gcgcgccgcc gccaaagatc
       61 tggatctgcg gcatcggcag caatgtggcc tcggggcctc cgatgtattc gtaaagcggc
      121 acgccctgcg cagccgccac cgcgtgcgcc gcggccatcg ataccgccag cgtggcgtta
      181 gcgcccagtc gggtcttcgc gtcggtgccg tcgagcgcga tcaggcgtgc gtcgagcgcg
      241 gcctgatcgt ctgctgcgtg gccgacgagc gcgcgggcga tctcaccgtt gacattggcg
      301 accgcccgca gcacgtccag cccgccgaac cgcgccgtcc cgtcgcgcag ttcgagcgcc
      361 tcgcgtgggc ccttcgatgc gcccgcggga acgatcgcgc ggcccattac tccgtcattc
      421 agcgtcactt ccgcctcgac cgtgggccgc ccgcggctgt cccagacgcg ccgcgcgtgg
      481 atcgatcgaa tcgtcgtgtt cattgttcga gttcctccgc ccaacgggtc cgcactgacc
      541 gcaggctggc gacgggatgc ttcaacaact cacgagccac gcgccgcacc tgctcccgct
      601 gccgttcttc ggtgacgtac tcgaccgggg acttgccgtc gatccgcgcg agaaagaggc
      661 ccggcaacag ggcagcggcc cgctgctcca atacgcccga gggttcccag tccacggatt
      721 gaaggtacgc cttggcgagc ccatcgtagg aagcgaggaa tgccggcgtt gcggccggag
      781 tccacagaca cttgagcagc agatgattca ggcaaaacgc aatgtcgaac gcggggtcac
      841 cccaccacgc acattcggca tcgaggaaca ccggtccgct cgctccgatc aggatgttct
      901 tcggactgac gtcgccgtgg accagcgcga cgcggttcga gcgcgtgacg tcgacgagat
      961 gctcgagccg ggacgcgagt tctggatgct tcttcgcagt agcgagcagg tacggttcga
     1021 gccggatgtc gaagaagatc gtgtcggtgt cgaattcggt ggcgagttcc ggccgacgtg
     1081 ccgagtagct gtggattttc ccgagcacgc gcccgacctc cgcggcggtt gccgggtcgg
     1141 cgtgtccatc gcgcagtcgg tgcttccaga ggctgaacgc gctcggcgga aggtagtcca
     1201 tgaccaggac gccgagcgag ggatgctggc ccagcacggt cggcgcacag cctgcgtatg
     1261 catcgctcgc cacgcgcatc cagcgcgctt cgtaacggtt gcgcgcgatc ggtgcacgcc
     1321 aatcggccgc cacgcgcagt tttgccagtg cacgtttggc gcaaaccgga ccttgcgcgg
     1381 tctggatgag ccagatctcg gacgacacac caccggtcat tggagtgccg acgatggcgc
     1441 ctgcatcagc aagaccgagt tcacgcaagg cctgcgcaaa ctctgcgggg acctcggtct
     1501 cagtcgccgc gctcatatcc ggcttcgacc agcttgacca gcgcggtgag cgtctggttg
     1561 atgggaactg gcacgcccag ttcgtggccc cggcgagcga tgaaaccgtt cagatggtcg
     1621 atctccgacg gcttccttca cgccatgtcc tgagcagttg aggaaaactg cgcgggcatc
     1681 gctaccgcga tccgttccat cgcatgcagc gacggctcca gttgcagcgc gatgccctct
     1741 gcctgcgcga gcgcgatcac ttcgcgcacg atcgcgtgct ggatctcgcg gacttcgagt
     1801 tgcgcggcga gcctggcata cggcgcctgc gcgagtgccg agatcgcgtt gtaggcgcag
     1861 ttgaccatca acttggacca gagctcagcc atgacgtcgt cagagaccgt caccttcacg
     1921 cccgcggcgc cgaacagcgt gaccaggtcc tgcagcacgc cggtaatggc gggatcgtgc
     1981 cgtgcatcgg cattcaatgc gccgatgacg aggtcccctc ggccgaaatg cctgacgctg
     2041 cctggacccg ccatcgcggt ggcaacgtat acgaccgcgg gcaccacggt ctggcgtacg
     2101 tgacgcgcga tgatcgcggc gttttcgaca ccgttctgca gactcagcac gacggtgtga
     2161 ggctccagca agggtcccag ttcgctcgcc accctatcgg tatcggtcga cttgacgcag
     2221 aagaggacca gatcggcgtc tcgcacggcg gccagctcgg tgctggcttc cgcgcgcagc
     2281 gtctcgacct gccccgccat gtcgagcctc aggccgtcgc gctggatggc ttcaacattc
     2341 ggcgggcgac cgatcaggac catgcgttga cctgcgcgcg caagcattgc gccataaaag
     2401 ccgccgacgg cgccggcgcc cacgacagcg atattgtgga atcggcttga catggcgaat
     2461 gatgctaaca ggcaagttgg tgacgattgt ccgaaccgag caagccgtac cgcccctggc
     2521 cgggtcttta gcatctcagt caggcgtcag ctgggcgtag ccgagtggca ttgctcgcat
     2581 tcctcattga ccggcaagcg ctcctctcaa ttaagggcag tggcattatc tccaggccca
     2641 actgccgcgt gcctgaacag gagacttgaa tgcttgctgc ctattacgaa cgagtcggcg
     2701 ccgcccggga tgtgctctca gtcgcggaga tcgacgcgcc cgcggcagga cccggcgaag
     2761 ttcgcgtcaa gctaacttgc tccggggtca acccctccga tgtcaagtct cgcggcggag
     2821 tgcgcagccg cgaactgccg tttcctcgaa ttgttccgca cagcgacggc gccggcgtga
     2881 ttgaccaggt tggcgcgggt gtctcagggc atcacattgg gcagcgcgtt tgggtgtgga
     2941 atgctgcgtg gggacgcgca ttcggaaccg ctgcgcaata cgtcgtgttg cccgaagcgc
     3001 aggcggtgcc gctccctgac aacatcgggt tcgccgaggg cgcctgcctg ggaattcccg
     3061 gcctgaccgc ttaccacgcg gtgcactcga acggcggtgt cgccggcaag agtgttctgg
     3121 tggcaggcgg cgcgggcgcc gtgggccact acgcggtgca gttcgcaaag ctcgccggcg
     3181 ctcggcttat agtcgctacg gtcagtaccg ctgccaaggc gacgcttgca cgggccgcgg
     3241 gtgcggacat gacgctcaat tacaaatcgg aggatgtcgg ccagcggtgc gttcaactca
     3301 cggacggaaa cggcatcgac cggatcattg agctagatct ggcgaccaat ctggcgacgg
     3361 acctcgccgc ggttcgccag gacggagaaa tcaccgtcta cggcagcacc gcgcccgaga
     3421 tcggcgtccc cttctttgcc tctgccctga agaacgtgcg ctaccagttc ttcatcgttt
     3481 acaagctatc actggcagat cgtgccacgg cgattgacga agtctccgcc ctgctcgacg
     3541 gcaacaggat tcagcacaac atcgcagcac gcctgccact gacgcacatc gccgaagcgc
     3601 acgaactcgt cgaaagcggc cgcgccaccg gaaatgtcgt tgtcgacatt cccgtgtaag
     3661 taacggcaac tcgcggccgc gcagagctca gcctcgactg ccgcttatgt agtgctcgag
     3721 ttgttcgatg atgaactgct gctcggagat gatgtctttc accaggtcgc cgatcgaaac
     3781 cagtccgatc agactgccgt tgtcgattac cggcagatgg cgcacgcggt tctcggtcat
     3841 gatggccatg cattcctccg tcgtctgcag cggtccaacg cagagcacct ccgtggtcat
     3901 gatgactgac acgggcgtct ccttcgacga tcgaccggcg agcgcgatct tgcgcgcgta
     3961 gtcgcgctcg ctgacgatgc cgacgatcct ttcgccttcc atcaccagca gcgcgccgat
     4021 gccctgctcc gccatcttct tgacagcgtc gtagaccgtg tcgttcggag cgaccgcatg
     4081 gacgaccttg ctcggcttac ccctcagaat ctgggcgata cctttcatcg gccatcctct
     4141 ctgtgttgat tatgcggttg ctgcctgaag ccggagcgat tgcagccaag ccagcggggc
     4201 gcgcaactgc tcaggtgtcg tggcctgtaa ctgacaggtt caatgctggc acaagtgcga
     4261 gccgctggca tcgcgcccag ccgtgagcgg gatggcgccg ccggagcgct gatgaaagtc
     4321 gccgccccgc aaggtgcgag caaacgaccg agaccggatt gccaggtgaa gtcgcgattc
     4381 ggcagagaca cggcttcggc gtcaaaactc gaccacctgg cgaactgccg tgccttcgcg
     4441 cagccggtcg aagccgagat tgatgtcggc aagtcgcagt cgaccggtca atagcctgtc
     4501 gaccggcaat cttccctgtc ggtaaagcga tatgtagcgc ggcaggtcgc gcaccggcac
     4561 acacgtcccg atgtagctgc ccttgatcgt gcgttcctcc gcgaccatat tgacgtgcgg
     4621 gaccgggaac acgtgagtgg gcggtggcag cccggcgctg atagtcgtgc cgccgcggcg
     4681 cgtgatccga taagcgagct ccatcgcctt gaccgaaccg gccatctcga gtgcgaagtc
     4741 gaccccgccg cgggtggcag ttcgaacgtc ctcgctgcag gattcggact tcgcgttgta
     4801 ggtgtcggtc gcccccagcg aagccgccag cgtcagcttg tcgtcgtcca ggtcgatcgc
     4861 gacgatgcgg ctcgcacctg cgcatactgc gccgatcaac gcggcgagac cgactccgcc
     4921 cagaccgatc acggcaacgg tctgcccgac cgatactctt gccgtgttga ccaccgcgcc
     4981 gacacccgtg agcacggcgc agccgaacag cgccgcttca gccaggggca gttccggatc
     5041 gatcttcacc agtgatcggc gggaaacggt ggcgtactcg gcgaacgcag aacagccgag
     5101 atggtggttc agtggctgcc catcccgggt caaccgcctg gccccggtga gcagggtgcc
     5161 ggctgtgttg gcgaccgcgc ccggctcgca tagcgccggc cggccttcag cacacggccc
     5221 gcaatgtccg cagctcggta cgaacaccat gacgacgtgg tcacccttgc gcaggtcgtc
     5281 gacacccgtt ccagcgtgct cgacgatgcc tgcggcctcg tgccccagca acatgggcat
     5341 cggccgtggc cggtcgccgt tgatcaccga caggtccgaa tgacagaggc cggccgcggc
     5401 gattttcacc agcacttcac cggttcccgg tggctcgagt tccacctgct cgatcgcgag
     5461 cgggcgcgac tcgctgtagg gagtcagcgc acccatctcg tgcaggacgg cgccattgat
     5521 tcgcatcggt ccctcccttc agtcgacttg ctgcagctcg ccgcgcgtca ccgccccacg
     5581 gtcgatcagg tacacgctgt cgagcatgtc gctcgagtgc gtcaggtctg actcggacag
     5641 gatcacggac aggccctcct tcttcagccg gccgatcacc tcgacgagcc gctggcacag
     5701 caccggtgct acgccttcga acggctcgtc gagcaacagc agccgcgtgc cgcacatcag
     5761 cgcgcgcgcc agcgcggcga gcttctgctg gccacccgat aactgcaacc cgcggcgcgc
     5821 tgcgaactca cgcgcctccg ggatcacgcg ataaacgaac gcaagccgct ccgacggatt
     5881 cgtcacgccg gttgcccacg cgggcagcaa gatgttttcc tcgacagtca gctcggggat
     5941 caggcggcgg tcctctggca tgtagccaat cccgagctgg gcacggcgat gtacgggcag
     6001 cgtgtttagc gcctgcccct cgaatgtgat cttgcccgaa gcggccggca gaatgcccat
     6061 gatgctgcgc atcagtgtgg tcttgccagc gccatttcgg ccgatcaacc cggcgaactc
     6121 gcccgcctcc accgcgaggt tcgcgttgcg caggatcgcg acgctgccga tcgcgacatt
     6181 cagctggtca atgcgcagca tggctcgcaa ccgacgtcgc gtggatggta ccgaccacat
     6241 aacggcggac ttcgtcgtcg ttgagcacct gctcgggcgc gccgtccgcg atgatgcggc
     6301 cttcgtagaa cgccagtacg cgctgcgcat agcggcggac tatatccatg tcgtgctcga
     6361 cgaatacgat cgtcacgcgc gcggcgcgca ccgcatccat cacccgatcc atgatcgcaa
     6421 atttctcgtc ggccgaaacg ccgctggttg gttcgtcaag cagcaggacc ttgggctgca
     6481 cgaccatcgc cattgcgatg tcgagcagct tgcgcacgcc ttccggcagc accgccgctg
     6541 gcttgtcgcg ccaatcctgg aggccgaagc gcacgagcgt ctcgttcgcg acggcatcga
     6601 tgcggtcggc tgcgatagcc tcgccgccct gccagcggct tgcgttcagc accacgacgc
     6661 cgagcgcgac cagcaggttg tcgcgcaccg tcaggctggc gaacagttgc ggaatctgga
     6721 acgagcggca catgccaagc cgggtgacct gtcgcggcac gagcttcacg atgtcgtgac
     6781 cggcatagcg gatcgaaccg cggtcgggct tcatgtaacc ggtcagcatg ttcaggaacg
     6841 tcgtcttgcc cgcgccgttg ccgccgatca gtccgaccac cgtgtcggcg tcgatgctga
     6901 cagtcagttc agccgcggcg gtcaccgcgc cgaagttctt gttcacgccg ctggtctcga
     6961 tgatcggggc gctcatgcgg ctttctttcg ccgaacgaac agcgaccaga gcccggcggg
     7021 catgaacatg atcaccagca gcatcgcggt gccaagcaca agttgccagg tgtcgggcgt
     7081 gtactggtac gcaacgctct tgatccattc aaacaccagc gaaccgatga acggagcgac
     7141 gacattgccc gtgccggaga ggatcgcaac gaacacgaat tcgcccgacg tggtccagta
     7201 agcgaggtcg ggatcgatat ggccgattga cagcgcgacc agcccgccgc cgacacccga
     7261 cagggacgca gcgagtacgt aattgagatg tacgatgcga cgcaccgacg cccccatgta
     7321 ctcgacgcgc agttcattgt cgcggatcgc cggtgcgaga cgtcccgtgt gagagccgac
     7381 aaaggcgtgg gttgcaacaa gcgctgcggc cacgaagatc gccgacaccg cgaacagccc
     7441 gtaaagatgc atgcccgccg gcaacgcgat gccggcaacc gtggcgtatg ccacattgaa
     7501 accatcgctg ctaccgagta ccgaagactt gatcagcaat ccatacagga tcatcgagaa
     7561 cgccaggctc agcatcgcaa agaaaatgct gcggtaggcc gccagcagga agccgagaat
     7621 gaaggcgaga atgcccgcga cgacgccgcc ggcaagcggc atcacaacgg cgtccgaggt
     7681 cagcagcgtt tcgggcaagc caaaaatccg cgtgattgat acagcggcgt acgcccccag
     7741 tgcatagaac agaccctgac cgaacgacac gagtccgcac cgcatcagca gcaaaagacc
     7801 gagcacgacc agccctttgc ccagcgcgag aatcaacagg ctctgcaccc agccaggcat
     7861 gaaccaaccg acgacgagcg tgaggatcaa accagagatg atcgcgatgg tggcgcgcgt
     7921 catatcttgc gtgcctcggc cgcgctgaag agtccccgcg gccgcgcgat cagcaccagc
     7981 gccatcacga ggtagatgct gaacagttcg acctctggcc agtagtgcac cgctgccgag
     8041 cggacgattc cgacgatcag cgcgccgagt gccgcgcccg gcaggctgcc gagtcccccg
     8101 atcaccacca cggcaaaggc caatacgatg acctcgacgc ccatcgccgg cacgattgat
     8161 atcttcggcg cgatcaacgc gccgcccagc gccgcgagca tcgtgcccag cgtgaacgtg
     8221 acgaggtaca tgcgtgcgac gttgattccc atcgcggcac tcacctcccg gtcatggatc
     8281 actgccagca gcagcttgcc ttgacgtgtg cgcgtcaaca cgaacgtcaa cgcggcgccg
     8341 acaacgatgg cgacgacaac cagcagcaag tcgtaaacgg tatacggcag gtcggccaga
     8401 tcgacgttgc caagcagacc atagggctga tagatggtgt aggactccac gccccagatc
     8461 agcttgatca cgtcctcgag gatcaggaat agcgagaacg tcaccaggat cagcaccacc
     8521 tcgtccttac cgtacatgaa tcgcagcagc ccgcgttcga tcagtgggcc ggccacgaga
     8581 ccgaccacga tcgcggccag cgccagcagc gcgaagctgc catagggatt gagcccagcg
     8641 ctgaaccagg caccgaccag cgtcgctgcg acatatgccc cgagcgcata caggctgcca
     8701 tgcgccaggt tcaggatgcg catgacgcca tagatcagcg tcagtcctac ggcgatgatg
     8761 aacaggtagg aggaatagac gatgccgtca accagcaccg acatgagccc ggtcatgggg
     8821 tcaaagagat ttaacgcgcg gcgccggtga aagcgccgcg caggtcactg gttcacttcg
     8881 cggtcttgaa ccctgacttg atccactctt cgctcttcac gccgtcgggc ggagtgatgc
     8941 gcgaagccgg atagcgtttg acgttgacga ccgtcacctt ggagccgtcg tgcttgacgg
     9001 tgccataggc ggtgccctgc acagcctgat ggcccttgcc gagcgacatg ctgaccgacc
     9061 cgcccggtcc ttcgaacgtc agattctcga acgcagcgac cacctgatcc tggttgggac
     9121 gcttgccgcc gttcgccgcc tgcgccttgt cccacgccgc tttgacaccg agaaacgcct
     9181 gcaccatctt gtacgcggga taactcggcg ccaccgcata gcgctcctca taggccttgc
     9241 ggaaccagcg gttcaactcg gtatcgggcg caaagacatt gaaaggcccg cgcgcgccga
     9301 tcaccgtgcc ttccggcact tgcgtgccga gtcgcacgag tgtcggttca ccggcagtca
     9361 tcacgatggt cgatttcttg aacaatccgc gtggtgcgcc ctgcaggatc aacgcctcgg
     9421 cgtctccgcc ccagaaactg ctgtggatga cgtcggcgcc actggtcagc aacgcagaaa
     9481 tctcggcccc gtactggcca gccagtagtt ttggcatctg tgaggtggcg atcttggcgt
     9541 ccgggttcag caccttgatg gccgcttcga agtcagtcca cgaatcctgc ccccacgcgt
     9601 agttctggtt gatcgccgct acggttttca atccgggctt catctcagtc acgtagagcg
     9661 ctgcggccgt gttgtccatc gtcgcggtcg cgccggtgcg gaacagatac ttgtagctgg
     9721 cgtcctcgaa tatgcgcggc gtgccgcagt cgaagaacac ggtgaggagc ttgagttcct
     9781 ccgcgaccgg tgccaccgcc agacagtcgc cgctggagat gtagccgatc accacgtcga
     9841 cgttgtcgcg ctggacaagg ttgcggtact cgttgacctg tttcgtcgtg ccaccggctt
     9901 cgtcgacaaa gacaacctcg atgggcgcac ctcccaaacc tgccgtgcca tacggcgccg
     9961 gcaccttgcc gccgttgagt gcctcgacga tcacttcggc gccattgcgc gccggcacgc
    10021 caaacggtcc ggcagcggcc cccgaaagga aggtcacgat gccgattttg atgggctttt
    10081 ccggtgctgc cttgtcctgc gcgccgacgg gcgcggacag cgcggccgcc gccattgcta
    10141 aacccgcagc gacgaacgcg cgcgtcagtt gccttcgaac ttcagtcatg tctcccctcc
    10201 tgtagttact cggccgtctt gtgcggtccg tccgcctttg gcagccagca ttcggactct
    10261 tcgtccatac cggcatggcg tcgtcagcat acggcgcttt gcaccgttgt ccagtccatg
    10321 agtggcattc agccaaatct ccttccgcgt cctggttttt gatgcaggta acctcaatcc
    10381 cgtcgctggt gtcgccgtca gcctcttgaa actggtcgca gctgcgacat ccagttcgcg
    10441 catggcgcga gttcgacggc gcacgtccca acccttcgtt tccggccgct cagtcgccgc
    10501 aattcctgat ttcagcttga ttcttgacag ttatgtattt gcgcaccgag gcgggtttca
    10561 cgagaacagc gggctactct gatcccgatt gcaacgcacg tcagttcgcc ggctccagcc
    10621 gcaggatggc gccgttcttt tcatcggtca gcagatagac aaagccgtcc ggtccggtgc
    10681 gtacgtcgcg ca
//
LOCUS       DATIRT010000378         1525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_357996, whole genome shotgun sequence.
ACCESSION   DATIRT010000378 DATIRT010000000
VERSION     DATIRT010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357996"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1508)
                     /locus_tag="VLN25_08545"
     CDS             complement(<1..1508)
                     /locus_tag="VLN25_08545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSQ10649.1"
                     /translation="MFQLGLTVLVALGVLLPFAPAGLPWLAFAIPVGFAQLVALLSRE
                     MPHHELVMLTWTMTMIGGGLTAQWLKRALSNNLSMRHRADLSARAQEQANAELNRSRE
                     QLRLALDAIDAGIADTNLITGERFFSARYFSILGYDDREAFLKDHRFSDALHPDDRSR
                     VLAARKKHTETGVPLREECRLRRADGGYVGVILRGESVRGTDGKPTRLVLSIVDNTEH
                     RIAQHRLIDSERRYRALVEASPSLIWVSDEKGRLTFVSDRACRDMFGVEPRELMGRHV
                     ADFNGPGFTRREFLRRFSPAMHGRSVFGMEAVLRNRQGEAVHVNVSAMPILNESGQVE
                     SVTGVCADITAMKQRERELNIAMRNQQAVFDAAGEGIVFVRNARIEGPNRALARMLGV
                     TREALVGQPVEEILATRSDWETIDQATRAASVRGEAAIHEVMLRANDGRSVWCQLTSR
                     QAGDGGAYILVLTDITSLKRREELAWHQANHDDLTGLPNRRQLGEHARRLLSV"
BASE COUNT          256 a          525 c          489 g          255 t
ORIGIN      
        1 accgacagca gacggcgggc gtgctcgccg agctgacggc gattcggcag cccggtcaga
       61 tcgtcgtggt tggcctgatg ccaggccagt tcctcgcgcc gcttcagcga ggtgatgtcg
      121 gtcagcacca ggatgtaggc accgccgtca ccggcctggc gcgatgtcag ctggcaccag
      181 acgctgcggc cgtcgttggc gcgcagcatc acttcatgaa tcgcggcctc gccgcgcacc
      241 gaggccgcgc gcgtcgcctg gtcgatggtt tcccagtcgg agcgagtcgc gaggatctcc
      301 tcgaccggct ggccaaccaa tgcttcgcga gtcactccca gcatgcgcgc gagcgcacga
      361 tttgggccct ctatgcgcgc attgcgcacg aatacgatgc cttcacctgc cgcgtcgaac
      421 acggcctgct ggttgcgcat cgcgatgttc agttcgcgct cgcgctgctt catcgcggtg
      481 atgtcggcgc aaactccggt caccgactcg acctgcccgg actcgttgag gatcggcatc
      541 gcactgacgt tgacatgcac ggcttcgccc tggcgattgc gcagtacggc ctccatgccg
      601 aacaccgacc gcccatgcat ggccggcgaa aagcgccgca ggaactcgcg ccgcgtgaat
      661 ccgggtccgt tgaaatccgc cacatggcgg cccatcagtt cccgcggctc gactccgaac
      721 atgtcgcgac aggcgcgatc cgagacgaac gtcaggcgcc ccttctcgtc ggatacccag
      781 atcagcgaag gcgacgcctc gaccagcgca cggtagcgcc gctcgctgtc gatgagccga
      841 tgctgcgcaa tgcggtgctc ggtgttgtcg acgatcgaca gcaccagtcg cgtcggcttg
      901 ccatcggtgc cgcgcaccga ctcgccccgc aggatgaccc cgacgtagcc gccgtctgcc
      961 cgtcgcaggc ggcattcttc ccgcagcggc acgccagtct cagtgtgctt cttgcgcgcc
     1021 gcgagcacac ggcttcgatc atcgggatgc agcgcatcgg agaaccgatg gtccttcagg
     1081 aaggcctcgc gatcgtcgta gccgaggatg ctgaagtagc gtgccgagaa gaagcgctcg
     1141 ccggtgatca ggtttgtgtc ggcgatgccg gcatcgattg catccagtgc gaggcgcagc
     1201 tgctcgcgac tccggttgag ctcggcattg gcctgctcct gcgcacgcgc cgacaggtcg
     1261 gcgcgatggc gcatggacag gttgttcgac aatgcccgct tcagccattg cgcagtcaac
     1321 ccgccgccga tcatcgtcat cgtccaggtc agcatgacga gttcgtgatg cggcatctcg
     1381 cgcgacagca gcgcgaccag ctgtgcgaag ccgaccggga tcgcgaacgc caaccacggc
     1441 aggccggcgg gcgcgaacgg cagcagcacg ccgagcgcaa cgagtacggt caggcccagc
     1501 tggaacatca gcccgccttc cgtcg
//
LOCUS       DATIRT010000379         6401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1341571, whole genome shotgun sequence.
ACCESSION   DATIRT010000379 DATIRT010000000
VERSION     DATIRT010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6401
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341571"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /gene="glmM"
                     /locus_tag="VLN25_08550"
     CDS             <1..854
                     /gene="glmM"
                     /locus_tag="VLN25_08550"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656257.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HSQ10650.1"
                     /translation="RTFPNDFDLKGLKLVVDSANGAAYHVAPHVFHELGADVVSHGAS
                     PNGLNINDGVGALHAEQLSHAVQVAEADLGIALDGDADRVLICDADGRLYNGDELLYV
                     IVRDSMRVGKVTGAVDTLMTNYALERRFSELGVGFARAKVGDRYVLEMLEEKGWQYGG
                     EGSGHLLCLDCHSTGDGIVSALQVLAAMKRSGRTLAQLTSDLVLLPQALVNARIERGF
                     DWKSHAPLIDEMSVVERELQGAGRVLIRPSGTEPVLRVMVEATEPEIAEAMAHRLAHR
                     VGAVVHG"
     gene            complement(1000..1317)
                     /locus_tag="VLN25_08555"
     CDS             complement(1000..1317)
                     /locus_tag="VLN25_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10651.1"
                     /translation="MKKMQTLHDRMMNATTPEERQKVMDEQRKEMQGSMAMMGQMMHG
                     SGTTGAMGTGMMGQQGKPADTGAQLQMMQKRMDMMQMMMQTMMDQQGMMAGPQGMGTA
                     PKK"
     gene            complement(2110..2313)
                     /locus_tag="VLN25_08560"
     CDS             complement(2110..2313)
                     /locus_tag="VLN25_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10652.1"
                     /translation="MKTLLTTGIGSIPLFAATAALGQTGQMMNDGFWGMGWMGGYGGV
                     WVPILLVVVVAAAVVVVMQRKSK"
     gene            3139..3477
                     /locus_tag="VLN25_08565"
     CDS             3139..3477
                     /locus_tag="VLN25_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10653.1"
                     /translation="MNRIPLLLIALLAAASTPARSQTVAPPTSSPTAKLNSHNASDAG
                     AAIPPLDYRSPFTAYQADKPQEPQSWRAVNDRVGAIGGWRVYAREAQPPIPDLPPNSS
                     SVGKPPPPVR"
     gene            3487..4905
                     /locus_tag="VLN25_08570"
     CDS             3487..4905
                     /locus_tag="VLN25_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSQ10654.1"
                     /translation="MRSSLSNRISGISGAGVLVLGLAGCTTFSADGGFGSVESSARSQ
                     LGKDVVWSRTDADSATVAARVAELLAKPLSVDDAVQIALINNKGLQAGYYELGLSESD
                     LVYAGRLPNPGFVFARKTQGDEVEIERLFTLNLMRLLTIPLAVEIESRRFEQTQQRVT
                     LEVLTLASVTRKAYFEAVAAEESVRYTRRAMESAEAGAELARRMAQAGNWGKLQQARE
                     QSFYADSALSLARAEQARIASRERLIRQMGLWGGQTAFELPERLPDLPKQADELPNIE
                     QQAMAQRLDVQSARRGTEAVAANLGLTKATRFINVLEFGVIHNSFNNAPVQRGYEIAI
                     EVPLFDWGNARVAQAEAIYGQAVNRLAETAVNARSEVRQAYLNYRASYDIARHYRDEI
                     VPIRKRISEENVLRYNGMLIGVFELLADARSQIASVNGYIEALRDFWLSKADLEMSLI
                     GRPDARNAPTAARGMAPAEAGH"
     gene            4923..6341
                     /locus_tag="VLN25_08575"
     CDS             4923..6341
                     /locus_tag="VLN25_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HSQ10655.1"
                     /translation="MNTRRQFFRGAGAVAAAVTAASVNRVALAALPEAVSQQKADTMP
                     PLSPQSGRPYRPVVTLNGWTLPWRMNSGVKEFHLVAEPVVREMAPGMKAHLWGYNGQS
                     PGPTIEVVEGDRVRIFVTNRLPEHTTIHWHGQRIPNGMDGVGGLNQKQIPPGKTFVYE
                     FVARRPGTFMYHPHADEMTQMAMGMMGSWVTHPKEKHPLIEEVDRDFIFLLNAYDIEP
                     GSYTPKIMTMLDFNLWSWNSRIFPGIDSLNVRLNDRVRVRIGNLTMTNHPIHLHGHEF
                     EVTGTDGGPVPLSARWPEVTTDIAVGQMRQIEFIADEEGDWAFHCHKSHHTMNAMGHD
                     VPTMIGVDHRGLSKKISQLIPDYMVMGERGMADMAEMEMPLPDNTVPMMTGEGPFGSV
                     EMGGMFSVVKVRADQKPNDYANPGWFKHPKGTRAYRYKQNMKEPARSRSAGRQSMPRI
                     QKPDTDVEVQVRKPTQHSKHQP"
BASE COUNT         1216 a         1995 c         1986 g         1204 t
ORIGIN      
        1 agcgcacgtt cccgaatgac ttcgatttaa agggactgaa gctcgtcgtc gacagcgcga
       61 acggtgcggc ctatcacgtc gccccgcacg tgttccacga actcggtgct gatgtcgtct
      121 cgcacggcgc gtcgcccaac gggctgaaca tcaatgacgg cgtcggcgcc ctgcatgccg
      181 agcagctttc gcatgcggtg caggtggccg aggcggatct cggcatcgcc ctcgacggcg
      241 atgccgatcg cgtgctgatc tgcgacgccg atgggcgcct ctacaacgga gatgaactgc
      301 tgtacgtgat cgtgcgcgat agcatgcgcg tgggcaaggt gaccggcgct gtcgacacgc
      361 tgatgacgaa ctacgcgctg gagcgccgct tttccgaact cggggtcggc tttgcgcggg
      421 ccaaggtcgg cgaccgctac gtcctggaaa tgctggagga gaagggctgg cagtacggcg
      481 gcgagggttc cggccacctg ctgtgcctcg attgtcacag cacgggagac ggcatcgtca
      541 gtgcgctgca ggtgctggcc gcgatgaagc gaagcggccg gacactggcc cagctgacct
      601 cggacctggt gctgctgccg caggcgctgg tcaacgcgcg catcgaacgg ggcttcgatt
      661 ggaagtccca tgcgcctctg atcgatgaga tgtcggttgt tgaacgcgag ttgcagggcg
      721 ccggtcgggt cctgatccga ccgtccggca ccgaacccgt gctgcgggtg atggtcgagg
      781 ccaccgaacc tgagattgcc gaggcgatgg cacaccgcct ggcccaccgg gtgggtgcgg
      841 tggtgcacgg gtagtcggtc gaccaagccg gcctcggccc gtttgcgctc tcagttgcga
      901 aagggagcta gccgcctcga gagttggcca ccgagaggac ttgcagttgc ccatcggtgg
      961 ccccttgcgc gacggctgga ctggccgccg ggaactcgat tacttcttgg gcgcggtgcc
     1021 catgccctgg ggaccggcca tcattccctg ctggtccatc atggtctgca tcatcatttg
     1081 catcatgtcc atgcgcttct gcatcatctg cagctgcgcg cccgtgtccg cgggcttgcc
     1141 ctgctgaccc atcatcccgg tgcccattgc acccgtcgtg ccgctgccat gcatcatctg
     1201 gcccatcatg gccatgcttc cctgcatttc ctttcgttgc tcgtccatca ctttctggcg
     1261 ttcctcaggg gtagtagcgt tcatcatccg atcgtgcagc gtctgcattt tcttcatgtg
     1321 ctcttccatc tgccccgtct ggctgctcgt gttcgcgggc gagcggggct gagtggcggt
     1381 ggcgcttgat gtcgacgccg gagctttcgc gtcctgcgcc agcggcgcgc cggcagcaaa
     1441 cgcgagcaca gtaccaatgg caattgcgac ttatcttttc atttgtatct ccaagttaag
     1501 cgattgaacg acacgcggtg cgcgcctaag gtgcacaggc catggggcaa agaactcagg
     1561 gaagatgtgg atgcgcgccg tcctcacgat tgagaggcgc ccagccttgg ccgcgcccta
     1621 caaagtcagg cggacgatgc ggtctgcaaa cgggacgccc ggtggcggat gcgcgcggca
     1681 cgcccttcgg gaaagcgacg cggaatgccg ccacaagcgg aactcgcggt gtgtctcgac
     1741 cagcatcgcg gcgcctgccg gcggctcctg taccaacgga agcacgcctg acaagggaag
     1801 gccgttgccg ggaaggtcct cgcagcctgg caccaggtcg tcgcccatag catcgccgct
     1861 cgcgtgcact gtcgtgtgcg acgtgagcga cgattgcgac accgtcgtta tgccatccca
     1921 gctgcatgcg tgcgcgatgc cgaggaagag cgcgaacagc cacagcccga gagccgcgga
     1981 ggcaagtccc tttcgatggc gacgcaacag gtttggcaca gtttccttcg agctatgaaa
     2041 tcgggaaccg acaccgcagt gggtcgcctg agtgtgaggc acgcacaggt accccgtacg
     2101 tacagctgtt cacttgctct ttcgttgcat gacgacgacc acggccgcgg caacgacgac
     2161 aacaagcagg atcggcaccc agaccccgcc atagccgccc atccagccca tgccccagaa
     2221 gccgtcattc atcatctgac cggtttgtcc cagggctgcg gttgcagcga acagcgggat
     2281 cgacccaatt ccagtagtaa gtagcgtctt cataattcct cccaaacata aagtgacgtg
     2341 cttgaagtac tgctctccag gcggcgcagg atgactgccg tcgcttcaac ggggcgcgac
     2401 gggctagaga caagcaccat caaaatcagc gtacccgccc gggaccccag tcgtcggtgc
     2461 ggtaacaaac ataggcatgg gctgtcctgc gtcgccaggg gtgatggccg ccgcatttgt
     2521 tgcgaccgca atgtgatttt gcggatccgg acgaaacacc ggatgcgaca gggaaccccg
     2581 cgtccgtcgt ctggactaag atcagatcgt gctcaagaag atcattaggg tcctggcctg
     2641 gctgctgatc gtcgctcttc ccgtacaggg cgtggcggca acggccatgc tccattgcgg
     2701 gcccggacac cacgggtcgg cggacacgcc ggtgcccggg gcgcatcacg atgatgtcgg
     2761 cgccgtttct cacgcgcacg aactgacgga tcccaccaca gacccgcggc accaccccga
     2821 cgatgaggcc cagccccccg tctcggatgc gagtggcttt gccaagtgtt ccgtgtgtgc
     2881 aacgtgctgt cttggagccg gctttccgat gggtagacgc gcagacgtgt tctccccgcc
     2941 tcactccctg cctctgctgt ccgcggtggt cccggttctc gagatctttg tccccggccc
     3001 agagcggcca cctcgctaca cccccgctta ggcgcttcgc gccgcgttgt gctgatgact
     3061 tgtcgttagt ggcaagtcgc ctgctgtttc tggctgctcg ctgtacggcg cagccggcca
     3121 acgacctacg agggttccat gaatcgaatt cctttgctgc tgattgcgct gcttgctgcg
     3181 gccagcacac ctgcgcgttc tcagaccgtc gcgccgccga catccagccc gacggcgaaa
     3241 ctgaattcac acaatgcatc cgatgcgggg gccgcgatcc cgcccttgga ctatcgctcg
     3301 ccattcactg cgtatcaggc ggacaagccg caggagccgc agtcctggcg cgctgtcaac
     3361 gatcgggtcg gagcaatcgg aggttggcgc gtctacgcac gcgaagcgca gccgccgatc
     3421 cctgatctgc cgccgaactc gtcgtccgtg ggcaagcccc cgccgccggt tcgctgaggt
     3481 gccgctatga ggtcatctct ttccaaccgg atcagtggta tcagtggggc cggcgtgctg
     3541 gtgctcggcc ttgcgggctg cacgacgttc tccgccgacg gcggtttcgg cagcgtcgag
     3601 tcgagtgcgc gcagccaact cgggaaggac gttgtctgga gccggaccga tgcggactcc
     3661 gcgacggtgg ccgcgcgcgt ggccgaactg ctcgcgaaac ccctgtcagt cgacgatgcg
     3721 gtccagattg cattgatcaa caacaagggc ttgcaggcgg gttactacga actcggcctc
     3781 tccgaatcgg atctggtgta cgccggaagg cttcccaacc ccggcttcgt gtttgcgcgc
     3841 aagacgcagg gcgacgaggt tgagatcgag cggctgttca cgctcaacct catgcggctg
     3901 ctcaccattc ctctcgcggt cgagatcgaa tcgaggcgat tcgaacagac gcagcagcga
     3961 gtgactctgg aagtgctcac gctggcgtcc gtgacgcgca aggcgtattt cgaggcggtt
     4021 gccgccgagg aatcggttcg ctacacgcgg cgcgcgatgg agtctgccga agcgggagcg
     4081 gaactcgcgc gccggatggc gcaggccggc aactggggca agctgcaaca ggcccgcgag
     4141 cagagcttct acgccgattc ggccttgagt ctggcgcgcg cagaacaggc ccgtatcgcg
     4201 tcgcgcgaac ggttgatccg acagatggga ctgtggggcg gccagactgc atttgagctg
     4261 cccgaacgcc tgcccgactt gccaaagcag gctgacgagt tacccaacat cgagcagcag
     4321 gccatggcgc agcggctcga cgttcagtcg gcacgccggg gtaccgaggc ggtagccgca
     4381 aacctcggac tgacgaaagc aactcgcttc atcaacgtgc tcgagttcgg cgtcatccac
     4441 aacagtttca acaatgcgcc ggtgcagcgg ggttacgaga tcgccatcga ggttccgctg
     4501 ttcgactggg gcaacgcgcg cgtcgcccaa gccgaggcaa tctacggaca ggcggtcaat
     4561 cgcctggccg aaacggccgt caatgcccgc tcggaagtcc gccaggccta tttgaactac
     4621 cgcgcgtcgt acgacatcgc ccgccattac cgtgacgaaa tcgtgccgat cagaaagcgt
     4681 atttccgaag aaaacgtcct tcgctacaac ggcatgctga tcggcgtgtt cgaactgctg
     4741 gccgatgctc gttcgcagat cgcaagcgtt aacggctaca tcgaggcgtt gcgcgacttc
     4801 tggctttcaa aggctgacct ggagatgtca ctgatcggtc gccccgacgc ccgcaatgct
     4861 ccgacggccg cccgcggcat ggcgcccgca gaggccggac actgagatcg ggagggatcc
     4921 acatgaacac tcgtcgacaa ttttttcggg gcgccggcgc agtagccgcg gccgtcactg
     4981 cggcatcggt caatcgtgtt gcgctggccg cattgcccga agcagtttcc cagcagaagg
     5041 ctgacacgat gccgccgctg tcgccacagt ccggccggcc ttaccggccc gtagtgaccc
     5101 tcaacggctg gacattgccc tggcgcatga attccggtgt caaggaattt cacctggtgg
     5161 ccgaaccggt ggtccgcgag atggcgcccg ggatgaaagc gcacctgtgg ggctacaacg
     5221 ggcaatcacc ggggccgacc atcgaggttg tcgaaggcga tcgcgtacga attttcgtca
     5281 ccaaccgact gcccgagcac accaccatcc actggcacgg ccagcggata ccgaacggca
     5341 tggacggcgt gggggggctg aaccagaagc agattcctcc gggcaagacc tttgtctacg
     5401 agtttgtcgc gcgacgtccc ggcaccttca tgtaccaccc ccacgccgat gaaatgacgc
     5461 agatggccat ggggatgatg gggtcatggg taacgcaccc caaggaaaag catccgctca
     5521 ttgaagaggt tgatcgggat ttcattttcc tgctcaacgc ttacgacatc gagccgggca
     5581 gttacacccc aaagatcatg accatgctcg attttaacct ctggagctgg aacagccgta
     5641 tctttccggg catcgattcg ctcaacgttc ggctcaatga cagggtgcgg gtgcggattg
     5701 gcaacctgac catgaccaac catccgattc atcttcatgg ccatgaattt gaggtgaccg
     5761 gcaccgatgg cggaccagtg ccattgtcgg cccgctggcc cgaggtcacc accgatatcg
     5821 ccgtcggcca gatgcggcag atcgaattta tcgccgatga ggagggtgac tgggcctttc
     5881 attgccataa aagccatcac accatgaatg ccatggggca tgacgtaccg accatgatcg
     5941 gcgtcgatca tcgggggcta agcaagaaaa tctcccagct catccctgat tacatggtga
     6001 tgggagaacg aggcatggct gatatggcag agatggagat gccccttcct gataacaccg
     6061 tgccaatgat gacgggggaa gggccgttcg gttctgtcga aatgggtggg atgttcagtg
     6121 tcgtcaaggt gcgggccgac cagaagccaa acgattatgc aaatcccgga tggttcaaac
     6181 accccaaggg gacaagagcc taccggtata aacagaatat gaaggagcct gcccgctccc
     6241 gctctgcagg aaggcagtcc atgccgcgta tccagaaacc cgataccgat gtcgaggtgc
     6301 aggttcgtaa gcctactcaa cactcaaagc atcaacccta gctgaagagg acatcataat
     6361 gatctcgaaa tttattctaa atcttatttt ctgcagcgtg g
//
LOCUS       DATIRT010000380         4083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_358137, whole genome shotgun sequence.
ACCESSION   DATIRT010000380 DATIRT010000000
VERSION     DATIRT010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358137"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VLN25_08580"
     CDS             complement(<1..398)
                     /locus_tag="VLN25_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HSQ10656.1"
                     /translation="MRALYNRFADTLARVIGHDLLALAARFGIAGVFWLSGRTKVEGW
                     LTVSDNAVALFADEYKVPLLSPELAAHLAAYAEHLFPILLVLGLATRASALALLGMTA
                     VIQVFVYPDAWPTHLVWATALLYLAGRGAGR"
     gene            complement(437..1234)
                     /locus_tag="VLN25_08585"
     CDS             complement(437..1234)
                     /locus_tag="VLN25_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HSQ10657.1"
                     /translation="MTMTMRLADYQDAFAHALLADDAVDVPPAVARLVAQPGFAVYRN
                     TVLKGCIDALQANFPAVARLVGDEWFRAAAALYVRRALPSAPTLLTYGRDFADFLSDF
                     APASELPYLAHVARVDRLWSEAHVAADDELLDPAALSRLAADDMGRLTLRPHAAVRWA
                     WCDEHPIYTLWSRNRDCSVDSAGEIDWRGEGVLLTRPQGAVMHQLLSREGVAFLRACA
                     DGLDIERAVAAALEVNPAADLSALIGQCLQAGTFADFETTTQKEVFR"
     gene            complement(1231..2070)
                     /locus_tag="VLN25_08590"
     CDS             complement(1231..2070)
                     /locus_tag="VLN25_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HSQ10658.1"
                     /translation="MQALTAGLGLKPQHYDGALATRSAGLWFEVHPENYLVEGGPRLA
                     WLDTLRERHPLSLHGVAMSLAADCAPDLTHLEQLARLVHRFEPALVSEHLAWSTWGGV
                     YRPDLLPVPRTQTALRRVVSNITQVQDRLQRPIALENPSHYLAFDEHEFDEVDFLVEI
                     ARRSGCTLLLDVNNVYVSANNLGYRPEGVIDAVPAGLISEVHLAGHSADPALGDALLI
                     DSHDAPVAPAVWSLYERLIARIGARPTLIERDEHLPDFADLLYERERAHRMMQDVSLA
                     RAA"
     gene            complement(2176..2457)
                     /locus_tag="VLN25_08595"
     CDS             complement(2176..2457)
                     /locus_tag="VLN25_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HSQ10659.1"
                     /translation="MKQTLAVTAFAFAAITAGAAMAQDKGAAIKGEKERCYGVSKAGQ
                     NDCAAGPGTTCAGTSKADYQANAWKYVPKGTCTTMTTPKGKGSLEPMKS"
     gene            2621..3196
                     /locus_tag="VLN25_08600"
     CDS             2621..3196
                     /locus_tag="VLN25_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSQ10660.1"
                     /translation="MNPANAAREARLHDLMVAALRGDAEAYAGFLRDTALFLRPFFRR
                     RMAQHPDDVEDLVQETLLAVHNKRHTYDPGQPVTAWMYAIARYKLIDLLRARAGREAL
                     HDPIDEDLDELGSEAEPTAHEARRDLRVLLAQLPDKQRLPIEYVKIEGLSVEETARRT
                     GLSVSAVKVGVHRGLKVLAARVLAARIRREQ"
     gene            3193..3834
                     /locus_tag="VLN25_08605"
     CDS             3193..3834
                     /locus_tag="VLN25_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1109 domain-containing protein"
                     /protein_id="HSQ10661.1"
                     /translation="MKTDDLIAALSREAEAVDPRAPVRRLLLASLLGVGAALPLMLWR
                     LGINPHLAADAQEPMLWIKWGFVALVAGASAVLVARLARPGVDARRAAQTVMAPLVAL
                     WGLAAVVLLAAAPAERMPLVMGSSWSSCPLNIAMLSLPAFVLLLAAVRTLAPTRLRRA
                     GAATGLLAGALGTLAYLLHCPELEAPFLAIWYVLGMAIPAVIGAAIGPRVLRW"
     gene            complement(3877..>4083)
                     /locus_tag="VLN25_08610"
     CDS             complement(3877..>4083)
                     /locus_tag="VLN25_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HSQ10662.1"
                     /translation="PWLMVSSLNLSPFVILVSITVWGGLWGIPGAFLAVPITACLAMI
                     FAEFAGTRPIAVLLSGNGEVAAPP"
BASE COUNT          682 a         1322 c         1404 g          675 t
ORIGIN      
        1 cgtccggcgc cgcggccggc gagatacagg agcgccgtag cccacaccag gtgtgttggc
       61 cacgcgtccg ggtagacgaa gacctggatc accgcggtca tgcccagcag cgcgagcgca
      121 gaggcgcgcg tggccagccc cagcaccagc aggatcggga acaggtgttc agcgtacgcc
      181 gccaggtggg cggccagctc gggggacagc aggggtacct tgtattcatc ggcaaacagc
      241 gccacggcgt tgtcactgac ggtcagccag ccctcgacct tggtgcggcc cgacagccag
      301 aacacgccgg caatgccgaa ccgtgcggcg agtgccagca gatcatggcc gatcactctg
      361 gcgagcgtat ccgcgaagcg gttgtaaagc gcgcgcattc cggtcggccc aacggtcatc
      421 gagcttgagg agttcatcat cggaagactt ccttttgagt cgtggtttcg aaatccgcga
      481 aggtaccggc ctgcaggcat tggccgatca gcgcggagag gtccgcggcg ggattgacct
      541 ccagcgctgc ggccacagcc cgctcgatat cgagtccgtc ggcacaggcc ctgaggaacg
      601 cgacaccttc acgcgacagc aactgatgca tgaccgcgcc ctgcgggcgc gtgagcagca
      661 ctccctcgcc gcgccagtcg atttcgccgg cggaatccac cgagcagtcg cgattgcggc
      721 tccacagcgt gtagatcggg tgctcgtcgc accacgccca gcgcacggca gcatgcggcc
      781 gcagcgtgag tcgccccatg tcgtcggcgg caagccggga gagcgcggca ggatcgagga
      841 gttcgtcgtc ggcagccaca tgcgcctcac tccagagccg atcgacgcgc gcgacgtgcg
      901 cgaggtacgg caactccgac gcgggcgcga agtcgctgag gaagtcggca aagtcgcgcc
      961 cgtatgtcag cagggtcggt gcggacggca gagcgcgccg gacatagagg gcggccgcgg
     1021 cgcggaacca ttcgtcaccg accaggcgcg cgaccgccgg aaagttcgcc tgcagcgcgt
     1081 cgatgcaacc cttcagcacc gtgttgcggt agacggcgaa gccgggctga gccacgagac
     1141 gtgcaacggc gggcggcaca tccaccgcat cgtctgcgag cagcgcgtgc gcaaaggcat
     1201 cctgatagtc ggcgagccgc atggtcatgg tcatgcggct cgtgccagcg agacgtcctg
     1261 catcatccgg tgtgcgcgct cgcgttcgta cagaagatct gcaaagtccg ggagatgttc
     1321 gtcccgctcg atcaatgtcg gccgcgcacc gatgcgggcg atcaatcgct cataaagtga
     1381 ccacacggcc ggcgccaccg gcgcgtcgtg gctgtcgatc agcagtgcgt cccccagtgc
     1441 cgggtcggcg ctgtggccgg ccaggtgaac ttccgagatc aatccggcgg gaacggcatc
     1501 aatgacacct tcgggccgat agccgaggtt gttcgcgctg acgtagacat tgttgacgtc
     1561 gagcagcagc gtgcagccgc tgcggcgtgc gatctcgacc aggaagtcga cctcgtcgaa
     1621 ctcgtgctcg tcgaacgcga ggtagtgcga cggattctcc agcgcgatcg ggcgctgcaa
     1681 ccggtcctgt acttgcgtga tgttgctgac gacgcgccgc agcgctgtct gtgtgcgcgg
     1741 cactggcagc aagtcgggcc ggtagacgcc gccccaggtg ctccacgcca ggtgctcgga
     1801 gaccagcgcc ggctcgaagc gatgaaccag acgggcgagc tgctcgaggt gcgtgaggtc
     1861 aggcgcgcaa tcggcagcca gcgacatcgc gacgccgtgc aacgacagcg gatggcgctc
     1921 gcgcaaagtg tcgagccacg cgaggcgcgg tccgccttcg accaggtaat tctcgggatg
     1981 cacctcgaac cacagtccgg cgctgcgagt ggcgagcgcg ccgtcgtagt gctgaggctt
     2041 gagtccgaga ccggcggtca gtgcttgcat gatgtgtaag gaaggtgccg cggcccgggc
     2101 gtttaatcaa cccgcaccgt tccaggagag gcagtcgtgc gggttggtga atcgcgcggg
     2161 ccgcggcagt ccttctcagc tcttcatcgg ctcgaggctg cccttgccct tcggcgtcgt
     2221 catcgtggtg caggtgccct tgggcacata cttccaggcg ttggcctggt agtcggcctt
     2281 cgatgtgccg gcgcaggtgg tacccggtcc ggccgcacaa tcgttctgcc cggccttgga
     2341 aacgccatag catcgttcct tttcgccctt gatcgctgcg cccttgtcct gggccatggc
     2401 ggcaccggcg gtaatcgcgg caaaggcgaa ggcggtgacg gcaagtgtct gcttcatgcg
     2461 gaaatctcct gacgggttgg gatgggccgg cgcgacgacc gcggcggcct gactgcttat
     2521 tcgttcggcg acacccgcgc gttacagtcc gccgcagaaa tatttttggg gttgcctgta
     2581 accgcgcgat cccggctgcc gaacccatga acaggcccac gtgaatccgg cgaacgcggc
     2641 ccgcgaagcg cgcctgcacg acctgatggt cgcagcactg cgcggcgacg ccgaggcgta
     2701 tgcaggattc ctgcgcgaca cggcgttgtt tcttcgtcca ttcttcaggc gccgcatggc
     2761 tcaacacccg gacgatgtcg aggacctggt gcaggaaacg ctgctggcgg tgcataacaa
     2821 gcgccatacc tacgatccgg gccagccggt cacggcctgg atgtatgcga tcgcccgcta
     2881 caagctgatc gacctgctgc gcgcacgtgc cggacgagag gcgctgcacg accccatcga
     2941 tgaagacctc gacgaactgg gctcggaagc cgagccgacg gcccatgagg ctcggcggga
     3001 cctgcgtgtg ctgctcgccc agttgcctga caagcaacgg ctgccgatcg agtacgtgaa
     3061 gatcgagggc ctgtcagtcg aggaaaccgc ccggcggacg gggctctcgg tctcggcagt
     3121 caaggtcgga gtgcaccgcg ggctaaaggt actggcagcc cgcgtgctgg cagcccggat
     3181 ccggcgcgaa caatgaagac cgacgacctg atcgccgcgc tgtcccggga ggccgaggcc
     3241 gtcgacccgc gcgcgccggt ccgacgcctg ttgcttgcat cactgctcgg tgtcggggcg
     3301 gcgctgccgc tgatgctgtg gcgcctaggg atcaatccgc acctcgcggc cgatgcgcag
     3361 gagccgatgt tatggatcaa gtggggcttc gttgcactcg tggccggggc cagtgccgtg
     3421 ctcgtggcac gactcgcgcg gcccggtgtc gacgcgcgcc gtgctgcgca gaccgtgatg
     3481 gctccgctgg tggccctctg ggggctcgca gccgtcgtgc tgctcgcggc ggcaccggcc
     3541 gagcggatgc cgctcgtgat gggatcaagc tggagttcgt gtccgctgaa tatcgcgatg
     3601 ctgtcgctgc ccgccttcgt gctgctgctc gccgccgtgc gtacgctggc gccgacgcgt
     3661 ctgcgacggg cgggtgcagc cacgggactc ctcgccgggg cgctgggtac gctcgcctat
     3721 ctgcttcact gccccgaact ggaggcgcct ttcctggcga tctggtacgt gcttggcatg
     3781 gcaattcctg ccgtcatcgg cgctgcgatc gggccacgcg tgctgcgctg gtgaatggtg
     3841 ttgaagctcg ctttgtagcg ctggcgatcc gaggtgtcac ggcggcgctg ccacttcgcc
     3901 gttgcctgac agcaatactg caatcggccg cgtccctgcg aactcggcga agatcatcgc
     3961 cagacaggcg gtgatcggga ccgccaggaa agcgccggga attccccaga gcccacccca
     4021 gaccgtgatg ctcaccagga tgacgaaggg gctcaggttc aacgagctga ccatcagcca
     4081 cgg
//
LOCUS       DATIRT010000381         1640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_554413, whole genome shotgun sequence.
ACCESSION   DATIRT010000381 DATIRT010000000
VERSION     DATIRT010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554413"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /gene="prpE"
                     /locus_tag="VLN25_08615"
                     /gene_synonym="yahU"
     CDS             <1..334
                     /gene="prpE"
                     /locus_tag="VLN25_08615"
                     /gene_synonym="yahU"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353244.1"
                     /note="catalyzes the formation of propionyl-CoA using
                     propionate as a substrate; PrpE from Ralstonia
                     solanacearum can produce acetyl-, propionyl-, butyryl- and
                     acrylyl-coenzyme A, and Salmonella enterica produces
                     propionyl- and butyryl-coenzyme A; not expressed in
                     Escherichia coli when grown on propionate/minimal media;
                     ATP-dependent; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HSQ10663.1"
                     /translation="NIAEVAVVGVADQLKGQVAMAFAILKDPSLADTPEKKLAHEGEM
                     MKTVDSQLGAVARPARVHFVNVLPKTRSGKLLRRSIQALCEGRDPGDLTTIEDPTALD
                     QIRSAVRP"
     gene            404..1147
                     /locus_tag="VLN25_08620"
     CDS             404..1147
                     /locus_tag="VLN25_08620"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HSQ10664.1"
                     /translation="MTFNYALSDVVLPPTSLIALTLVGVAILGRRRAIGLTLILGSQL
                     LLLALSLPLVANALARSLEPPPVTAAQLKQAQVIVILAGGNNRGSPEWGGETVKTYTL
                     QRLRYGARLARETGLPIYVTGGRPEGGRFAEAELMRQVLVQEYGLEVRWVDVTAMTTG
                     ENALAAARDLKPEGLTRIALVTDAVHMPRSRQVFEAVGFEVIDCPTAYAGQRPFAPTQ
                     LVPGASALHQSQAALREWVSRGVYVLRYR"
     gene            1185..1583
                     /locus_tag="VLN25_08625"
     CDS             1185..1583
                     /locus_tag="VLN25_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HSQ10665.1"
                     /translation="MKTKPMLTLDDAQRIAAAAEAEATAHQWNVVIAICDDGGHLVLL
                     HRLDGVAPVSSMIAPAKARAAALGKRETKLYEDMINGGRTSFLSAPLDGMLEGGVPII
                     VDGHVVGAVGVSGVKSVEDAQIAKAGIAAL"
BASE COUNT          274 a          532 c          566 g          268 t
ORIGIN      
        1 gaacatcgcc gaggtcgccg tcgtcggtgt tgccgatcag ctgaaggggc aggtcgcgat
       61 ggctttcgcg atcctgaagg acccgagcct ggccgacacg ccggagaaga agctcgcgca
      121 cgaaggcgag atgatgaaga ccgtcgacag ccagctcggt gcggtcgcgc gtccggcacg
      181 cgtgcacttc gtcaacgtcc tgccaaagac acgctccggc aagttgctgc gacgctcgat
      241 ccaggcgctg tgcgaaggcc gcgatccggg cgacctgacg acgatcgagg atccgaccgc
      301 gctcgaccag atccggagtg ctgtgcgtcc ctaggcctac cagcgcctgc cagggccgcg
      361 ggctcgcgta agctcgcggc cgttttcttc tgcaggtcac cgtgtgacgt tcaactacgc
      421 cttgtccgat gtcgtactgc cgccgacgtc gctgatcgcg ctgacgctgg tcggcgtcgc
      481 catcctcgga cggcggcgcg caatcgggct gacgctgatt ctcggttcgc agttgctgct
      541 gctggcgcta tcgctgccgc tggtggccaa tgcgctcgcg cgctcgctcg aaccgccccc
      601 cgtgacggcg gcgcaactga agcaggcgca ggtcatcgtc attctcgcgg gcggcaacaa
      661 ccgcggctcg ccggagtggg gcggcgagac tgtcaagacc tacacgctgc aacgattgcg
      721 ctacggcgcg cgactggcgc gcgagaccgg cctgccgatc tatgtcaccg gcggccgccc
      781 cgaaggcggg cggtttgcag aggctgaact gatgcgccag gtgctggtgc aggagtacgg
      841 cctcgaggtc cgctgggtcg acgtgaccgc gatgacgacc ggtgaaaacg cgctcgccgc
      901 ggcccgggac ctcaagcccg aaggcctgac gcgcattgcg ctggtgaccg acgccgtaca
      961 catgccgcgc tcgcgccagg ttttcgaggc agtgggcttc gaggtgatcg actgcccgac
     1021 cgcgtatgca ggacagcgtc cgttcgcgcc gacccagctg gtgcccggcg cctccgcgtt
     1081 gcatcagtct caggcagcgc tgcgcgaatg ggtctcgcgc ggtgtttacg tgctgcgcta
     1141 tcggtgaact gaggatgaac tgaggaagta gcgaggagac gacgatgaaa accaagccca
     1201 tgctcacgct cgacgatgcc cagcggattg ccgcggcggc cgaagccgag gcgaccgccc
     1261 atcaatggaa cgtagtgatc gccatttgcg acgacggcgg gcacctggtg ctgctgcatc
     1321 gccttgacgg agtggcgcct gtctcgtcga tgatcgcgcc cgcgaaggcg cgcgccgccg
     1381 cgctcggcaa gcgcgaaacc aagctgtatg aggacatgat caatggcggg cgtacctcat
     1441 tcctgtcagc gccgctcgac ggcatgctcg agggcggcgt accaatcatc gtcgacggtc
     1501 acgtcgtcgg tgccgtcggt gtttcgggcg tgaagtcggt cgaggacgca cagatcgcca
     1561 aggcgggcat cgcggcgctg taggccgtcg ctagacctgc gcagcgcgcg ggatcagcac
     1621 gcagtctcag cacaggaatg
//
LOCUS       DATIRT010000382         7449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1145781, whole genome shotgun sequence.
ACCESSION   DATIRT010000382 DATIRT010000000
VERSION     DATIRT010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7449
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145781"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1844)
                     /locus_tag="VLN25_08630"
     CDS             complement(<1..1844)
                     /locus_tag="VLN25_08630"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HSQ10666.1"
                     /translation="MFGKRVPMTLQSEAPECGIACVAMIASYYGFRTDLSAMRLRLAP
                     SMKGVTLKHISAIADTMKLAARGVKVPLEGLSQLKLPAILHWDMNHFVVLTKTSGDRI
                     TVNDPARGRRVLTLREASDHYTGVAMELTPTTGFAERDEREKISAWQLVKTATGLRSA
                     VTQVLLLSLVLEVLAIASPFFLQLVVDRVVVGRDRDLLAVLGIGFGILAVMIAIITAL
                     RSWLGVYISTRLNLQLLDTLFARLLRLPLAWFEKRHIGDIVSRFRSVDAIQRTLSLTF
                     LETVMDGVMVIVTLAVMSWYSTTLTIIVVIAAVLYGSLRWAMYGPQRRATDERIVHEA
                     KAATHFIETLRGMMAIKLNLREGERRAAYLNHVVDQTNADVRVQNLALLQRAGNVLIF
                     GLENVIVIWLGANAVLEGQLSVGMLFAFLLFKLLFITRVNNLVDKTIEFRMLDLHADR
                     VADIALAPTESAAELPAVARADAPAGAFVLESRDLGFAYGIEGFVFRGMGFSAKPGET
                     VAIVGPSGSGKTTLVKVLAGLLDRTEGTLTAAGRDVRDWDKGAYRSRIGVVMQDDHLF
                     VGTIEDNISFFDPQHDPARVRECSRLAMIDEDISAMPMGYNTIVGSLGV"
     gene            complement(1920..3203)
                     /locus_tag="VLN25_08635"
     CDS             complement(1920..3203)
                     /locus_tag="VLN25_08635"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HSQ10667.1"
                     /translation="MRLARPWTMAAQPKSLFRQEAIDAQREKLLGEVSLAQPVPMWIF
                     TTLAVAFAAALVAFSIWGEYARRERVDGFLALDSGAARILAPEPGTVAELLAREGDEV
                     EAGQPLIRLSFERGTASGVTSGELVQREIGERIAGLEREQQQVKLLGQQQADALRRKI
                     ADLERELLQFASELRAQETRLASARSEFQRAEELFKDKFYSESKLIEFRNNVLDQQVK
                     VEALKRERSGVERELTAARSDLPSVELKNQTQLDQIRRQMSELQQGSVQEEAKRENVI
                     RAPVAGVLTNIAAARGETVAESAPLAVVVPKGSGLHAQLLVPTRAIGFVQPGQAVVLR
                     YDAFPFQRFGQYRGTVERVSRTVWSAGEKVGQLVVKEPVYRVDVKLDAQYVAAGGQQL
                     PLRSGMMVNADILQEKRKVWEWVFEPVLSLKERFQ"
     gene            3365..3730
                     /locus_tag="VLN25_08640"
     CDS             3365..3730
                     /locus_tag="VLN25_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10668.1"
                     /translation="MLTLRSLAFAATFMAVPLGMAYAQDADPAVARTRAAADAAHEQL
                     VDRYRAVWSRLSPAQKAEFGSRERAWLNGGRAEQERLCVESQPAASIALATQACRLAV
                     TERRLAILPATVLQASARP"
     gene            complement(3800..4384)
                     /locus_tag="VLN25_08645"
     CDS             complement(3800..4384)
                     /locus_tag="VLN25_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HSQ10669.1"
                     /translation="MKLLAWITAFLISASIAPAFAQKVDASKVPEEQRSKLGLYILPK
                     QAPAFLKSQNGKVLFLDVRTRAEAQFLGMADGVDALVPYVEFQEFMTDWDENRGFYKL
                     EPFSDFAPEVERRLKAKGLGKNDTILLICRAGERSSRAADLLESLGYTKVYTVLNGLE
                     GELSAKGRRNVNGWKNDGLPWSYELDRKKMYFPR"
     gene            4565..5536
                     /locus_tag="VLN25_08650"
     CDS             4565..5536
                     /locus_tag="VLN25_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HSQ10670.1"
                     /translation="MALPGFDQFRAAVACADRAGVSSRAMADPTPRLTAYVLLAGSMA
                     LVGSYVALSKPLTAAIPVFALAFLRFAIAAIAMVPWTVPARTDTALTRTEHRLFFVMS
                     FFGNFLFSICMLYGVSMTTATAAGVILATLPAVVALLSWLILHERLSGRVMLAVVLAV
                     GGVGLLQLARPDDPAARGTAWLGTLLMFGAVVCEATYVIVAKRLAATRAPLRVSALVN
                     LWGLVLITPFGLWQLARFDLGSLSAPIWGLLVFYSLAASLFAVWMWVAGLKHVPASQA
                     GVFTVALPLAATAVGVFILGESFTPIHAVSLALATAGVVLITTGRQP"
     gene            5634..6161
                     /locus_tag="VLN25_08655"
     CDS             5634..6161
                     /locus_tag="VLN25_08655"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSQ10671.1"
                     /translation="MTSDHVRIADSLPPQVRIAVTVLAINAKSRLLEQRRPHAQRRPP
                     LVAVLLSPYPEARAAAMAVIARHLSAPVQRVDWTRLLSPYVHETELNVERLFDAAEHA
                     GVLLLFDEADALFGPHGRPIDLADSAHEACTEALLARMAQYDGPVCFTASHRRHLHPR
                     VLATADPVIELTSSP"
     gene            complement(6153..>7449)
                     /locus_tag="VLN25_08660"
     CDS             complement(6153..>7449)
                     /locus_tag="VLN25_08660"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HSQ10672.1"
                     /translation="GRLAQLGLAAGELAVGGAVQGLRRLRHGDASAPRFSVATVRRLV
                     DRLAHLRGAAMKIGQLISLEAEGLLPPELVIALETLRSQAAPMPLEQLRRALGRAYGS
                     GWERRFAQFDEVPIASASIGQVHRARTADGRDIALKVQYPGVARSIASDVDNVATLLR
                     IAQVLPLGIDVEDIAREAKRQLAQEADYLAESGFLERYARLVADEAQIVVPRVHWDYT
                     TARVMAMDYMNGEPLDSLAVSTSQKERDSVGALLQRLMFRELFEFRVMQTDPNFANYL
                     YQPDTGRLVLLDFGATQEFSPEFSERFRDVTRAIVAGDRAGVERGARNIGYIAEDAAP
                     EVVTAALDMIELVCEPLRMTGAYDYGASDLSRRAREMGIDLAFKRGLLRMPPSDTMFL
                     HRKLVGVFLLLARLGARVPTGRILWPFLAPGAASKSASG"
BASE COUNT         1212 a         2545 c         2363 g         1329 t
ORIGIN      
        1 acgccaaggc tgccgacgat ggtgttgtag cccattggca tggccgaaat atcttcatcg
       61 atcatcgcca gccgggagca ctcgcgcacc cgcgcggggt cgtgctgagg atcgaagaaa
      121 ctgatgttgt cctcgatcgt gccgacaaac aggtgatcgt cctgcatcac gacgccaata
      181 cggctgcggt aggcgccctt gtcccagtcg cgaacgtcgc gcccggcggc ggtcagggtg
      241 ccctcggtac ggtcgagcag gcccgccagc accttgacca gagttgtctt tccactgccg
      301 gacggtccga cgatagccac cgtttcgcct ggcttggcac tgaaccccat gccgcggaac
      361 acgaagccct cgatgccata ggcgaatccg aggtcgcggg actccagcac gaaggctccg
      421 gccggagcgt cggcccgtgc gacagccggc agttccgccg cgctctcggt gggggccagc
      481 gcgatgtcgg caacgcggtc ggcatgcagg tcgagcatgc ggaactcgat cgtcttgtcg
      541 accagattgt tcacacgcgt gatgaagagc agcttgaaca gcaggaacgc gaacagcatg
      601 ccgaccgaga gctggccctc gagcacggcg ttggcgccga gccagatcac gatcacattt
      661 tccagcccga agatcagcac gttgccggca cgctgcagca gcgcgaggtt ctgcacgcgc
      721 acgtcggcgt tggtctggtc gaccacgtgg ttcaggtagg cggcgcggcg ctcgccctcc
      781 cgcaggttga gcttgatcgc catcatcccg cgcagcgtct cgatgaagtg tgtggcggcc
      841 ttggcctcgt gcacgatgcg ctcgtcggtc gctcgccgct gcggtccgta catggcccag
      901 cgcaggctgc cataaagcac cgcggctatc acgacgatga tcgtcaacgt cgtgctgtac
      961 caggacatga cggccagcgt gacgatcacc atcacgccgt ccatcacggt ctcgaggaac
     1021 gtcagcgaca gcgtgcgctg gatcgcatcg accgagcgaa agcgcgagac gatgtcaccg
     1081 atgtgccgct tctcgaacca tgccagcggc agcctcagca gccgtgcgaa cagggtgtcg
     1141 agcagttgca gattgagtcg cgtgctgatg tagacgccca gccaggatcg aagcgccgtg
     1201 atgatggcga tcatcactgc cagtatcccg aaaccgatgc cgagcaccgc cagcaggtca
     1261 cggtcgcgcc cgacgacgac gcgatccacc acgagctgca ggaaaaacgg cgatgcaatc
     1321 gcgagcactt ccagtaccag cgaaagcaga agcacctgcg tgacggcgga acgcaggccg
     1381 gtcgcggtct tcacgagctg ccaggcgctg atcttctcgc gctcatcgcg ctcggcgaag
     1441 cccgtcgtgg gcgtgagttc catcgccacg ccggtgtagt gatccgacgc ctctctcagc
     1501 gtcagcacgc gccggccgcg cgccgggtca ttgacggtga tgcggtcgcc cgatgtcttc
     1561 gtcagcacga cgaagtgatt catatcccag tgaaggatcg ccggcaactt gagctggctc
     1621 aggccctcga gcggtacctt cactccgcgc gcggcgagct tcatcgtgtc ggcaatggcg
     1681 ctgatgtgct tgagcgtcac gcccttcatc gacggcgcca gccgcagccg catggctgac
     1741 aggtcggttc ggaaaccgta gtagctggcg atcatcgcca cgcaggcgat gccgcattcc
     1801 ggcgcttcgc tctgcagggt catcggcacg cgcttaccga acatggcgcc cccttacgag
     1861 acgcgtggcg gccgatgtat caacgaggca tggcacgacg acgccgtgcg acgtacgcct
     1921 cactgaaacc tctctttcag cgacagcacc ggctcgaaca cccactccca caccttgcgc
     1981 ttctcctgca ggatgtcggc gttgaccatc attccggagc gcaacggcag ctgctggccg
     2041 ccggccgcga catactgggc gtcgagcttg acgtcgacgc ggtacacggg ttctttcacc
     2101 acgagctggc cgaccttttc acccgccgac cagacggtgc ggctgacgcg ctcgaccgtg
     2161 ccgcggtact gcccaaagcg ctggaacgga aatgcgtcgt accgaagaac gaccgcctgc
     2221 cccggttgca cgaaaccgat cgcgcgcgtc ggcaccagca gctgcgcgtg cagcccgctg
     2281 cctttgggca cgaccactgc gagcggtgca ctctccgcca cggtctcgcc gcgcgcggcc
     2341 gcgatgttcg tcagcacacc cgccactggc gcgcggatca cgttctcgcg cttggcttct
     2401 tcctgcacgc taccctgctg cagttcgctc atctgacggc ggatctggtc gagttgcgtc
     2461 tgattcttca gctcgaccga cggcagatcc gaccgtgccg ccgtcagttc gcgctcgact
     2521 ccggaccgtt cccgcttcag cgcctcgacc ttgacctgct ggtcgagcac gttgttgcgg
     2581 aactcgatca gcttcgactc cgaatagaac ttgtccttga acagttcctc ggcgcgctgg
     2641 aactcgctgc gtgccgaggc gagccgcgtt tcctgcgcac gcagttccga ggcgaactga
     2701 aggagttcgc gctccaggtc tgcgatcttc cggcgcagcg catcggcctg ctgctggccc
     2761 agaagcttga cctgctgctg ctcccgctcc agtcccgcga tgcgttcccc gatctcgcgc
     2821 tggacgagtt ccccggaagt cacaccggag gcggtgcccc gttcgaacga aaggcggatc
     2881 agcggctgcc cggcttcgac ctcgtcaccc tcccgagcca gcagttcggc gaccgtgccg
     2941 ggttccgggg cgaggatgcg cgccgcaccc gaatccaatg ccaggaagcc gtcgacacgc
     3001 tcgcgccgcg cgtactcgcc ccagatcgag aacgccacca gggccgcggc gaaggcgacc
     3061 gccagcgtgg tgaagatcca catcggaacc ggctgcgcga gcgacacctc gccaagcagc
     3121 ttttcgcgct gcgcgtcgat cgcttcttgc cggaagagtg acttgggctg tgcggccatg
     3181 gtccaggggc gggcgaggcg cattatgggg cgggtctccg ccggcgtggt gagtgcccgc
     3241 acacgtggac tcacaaatga acgctaggcg tcgcgttata tcgacataac ggacatgttg
     3301 ctgcggtgga ggcatcgggc cctacgctgc gcggacccct tccgccctcc ggagcttcac
     3361 cgccatgctc accctgcgct cactcgcttt cgctgcaacc ttcatggcgg tgccgctggg
     3421 gatggcgtat gcacaggacg ctgatcctgc ggtggcaagg acgcgcgcag ccgctgacgc
     3481 cgcgcatgag cagctggtcg atcggtaccg cgcggtctgg tccaggttga gcccggcgca
     3541 gaaggccgag ttcggatcgc gcgagcgtgc ctggttgaat gggggtcgcg ccgaacagga
     3601 gcggctctgc gtggagtcgc aaccggcggc atcgatcgcc ctggcgacgc aagcctgtcg
     3661 cctggcggtg accgagcggc gcctcgcgat actccccgca acggtgcttc aggcctccgc
     3721 gcgcccctag cccagcccga tttcgtcgat cacttccact tgatcgagca gccgctgctc
     3781 ggacggcccg cgccgatgtt cagcggggga agtacatctt cttgcggtcg agctcgtagc
     3841 tccagggaag cccgtcgttc ttccagccgt tgacattgcg ccggcccttg gccgacagct
     3901 cgccctcgag cccgttcagc acggtgtaca ccttcgtgta accgagcgac tccagcaggt
     3961 ccgcggcacg cgacgaccgc tcgcctgcgc gacagatcag caggatcgtg tcgttcttgc
     4021 cgaggccctt ggccttcagc cgccgctcga cctccggcgc gaagtcactg aacggctcga
     4081 gtttgtagaa gccgcggttc tcgtcccagt cggtcatgaa ctcctggaac tcgacatacg
     4141 gcaccagcgc gtcaacccca tcggccatgc ccagaaactg cgcctcggcg cgcgtgcgca
     4201 catccagaaa cagtaccttg ccgttctgcg atttgaggaa ggccggagcc tgcttcggca
     4261 ggatataaag gcccagcttc gagcgctgtt cttccggcac tttcgacgca tccaccttct
     4321 gcgcgaaggc cggcgcaatg gacgcactga tgagaaatgc ggtgatccag gcgaggagct
     4381 tcaatcgttt ctccggcgtt cggggctccg tgccagacgg agtccaagag tgttcttgaa
     4441 tgataggggg cgttgccggc ggcggggctt ccgcacaaca tacctttata acctaaatca
     4501 gtgatacccc tacaggtatc cgtataagca attttacaga ctgacgggtc cggatcgacg
     4561 gcgcatggcg ttgccgggat tcgatcagtt ccgcgcggcc gtggcctgcg ccgatcgcgc
     4621 tggcgtatcg tcgcgcgcga tggctgatcc gactccgcgc cttactgcct atgtcctgct
     4681 ggccggctcg atggctcttg tcggcagcta cgttgccctt tcgaagccgc tgaccgcggc
     4741 catcccggtc ttcgcgctgg ccttcctgcg ctttgcgatc gccgccatcg ccatggtccc
     4801 gtggactgta cccgcgcgta ccgacactgc gttgacgcgc accgagcatc ggctgttctt
     4861 cgtcatgtcg ttcttcggca atttcctgtt ctcgatctgc atgctctatg gcgtgtcgat
     4921 gacgaccgcg accgccgccg gcgtgatcct cgccacgttg cccgcggtcg tcgcgctgct
     4981 gtcctggctg atcctgcatg agcgcctgag cggtcgcgtg atgctggcgg tggtcctggc
     5041 cgtcggcggc gtcggattgc tgcaactcgc gcgccccgat gatcctgccg cgcgcggcac
     5101 cgcctggctc ggcactctct tgatgttcgg cgccgtcgtc tgcgaagcga cctacgtgat
     5161 cgtcgcgaag cggctcgccg cgacgcgggc gccgctgcgc gtgtcggcgt tggtgaacct
     5221 gtggggactc gtgctgatta cgcctttcgg gctgtggcag ctggcgcgct ttgatctcgg
     5281 ctcactgagc gcaccgatct ggggcctgct ggtgttctat tcgctggcgg ccagcctgtt
     5341 cgcggtctgg atgtgggttg ccggactgaa gcatgtaccc gccagccagg ccggtgtgtt
     5401 cacggtcgcc ctgcccttgg ccgcgactgc cgtcggcgtg ttcattctgg gcgaatcctt
     5461 cactccgatc cacgccgtgt ctctcgcgct tgcgaccgcc ggtgtcgtgc tgatcaccac
     5521 gggtcgacag ccgtgaccgg ccaaacggct tcccccgcgt gcgcgatcgc gcaggttggc
     5581 gattccgacc agcggcgacg cgatcggcgc gtggcgttcc gtatcgttgc tgcatgacct
     5641 cggatcacgt caggattgcc gacagcctcc ccccccaggt gcggatcgcc gtgaccgtgc
     5701 tggcgatcaa cgccaaatca cggctgctcg aacaacgccg gccgcacgcg caacgccggc
     5761 cgccgctggt tgcggtgctg ctgagcccct atcccgaagc acgggcggcg gccatggctg
     5821 tcatcgcgcg tcacctgagt gcaccagtgc agcgagtcga ctggacgcgg ctgctgtcgc
     5881 cctatgtgca cgagaccgaa ctgaatgtcg agcggctctt cgacgcggcc gagcacgcgg
     5941 gcgtgctgct gctcttcgac gaagctgatg cgctgttcgg cccgcacggc cgtccgatcg
     6001 acctcgctga cagcgcgcat gaagcctgca ccgaagcgct gcttgcgcgc atggcgcagt
     6061 acgacgggcc ggtgtgcttc accgcgagcc atcgccgcca tctgcatccg cgcgtgctgg
     6121 ccaccgcgga tcctgtcatc gaactgacca gttcaccctg acgccgattt cgaagccgct
     6181 ccgggggcca gaaacggcca gaggatgcgg cccgtcggaa cgcgcgcgcc gagccgagcg
     6241 agcagcagga agacaccaac cagcttacgg tgcaggaaca tcgtgtccga gggcggcatg
     6301 cgcagcagtc cgcgcttgaa ggccaggtcg atccccatct cacgcgctcg acgcgacagg
     6361 tccgacgcgc cgtagtcgta ggcgccggtc atacgcagcg gctcacagac cagttcgatc
     6421 atgtcgagcg cggccgtgac gacttccgga gcggcgtctt cggcgatgta gccgatgttt
     6481 cgggctccgc gttcgacgcc ggcccggtcg cccgcgacga tcgcgcgcgt gacgtcgcga
     6541 aatcgctccg agaattcagg gctgaactcc tgcgtggcgc cgaagtcgag cagcacaagc
     6601 cgcccagtgt ccggctggta cagatagttc gcgaagttcg gatcggtctg catcacgcga
     6661 aactcgaaca gttcgcggaa catcagcctc tgcagcagtg cacccacgct gtcgcgctcc
     6721 ttctgcgagg tgctgaccgc gagcgaatcc agtggctcgc cattcatata gtccatggcc
     6781 ataacgcgtg ccgtcgtgta gtcccagtgc acgcgcggca ccacgatctg cgcctcgtcg
     6841 gccaccagac gggcgtagcg ttcgaggaag cccgattcgg caagatagtc ggcctcctgc
     6901 gccagttggc gcttggcctc gcgggcgatg tcctcgacat cgatgcccag tggcagcacc
     6961 tgcgcgatcc gcagcagtgt tgcgacgttg tcgacgtcac tggcgatcga gcgtgccacc
     7021 cccgggtact gcaccttgag cgcgatgtcg cgaccgtccg ccgtgcgtgc gcgatgcacc
     7081 tggccaatcg aggcagacgc gatcggcacc tcgtcgaact gcgcgaagcg cctctcccag
     7141 cctgagccat aggcccggcc cagcgcgcgc cgcagttgct cgagcggcat cggtgcagcc
     7201 tgcgagcgca acgtctccag cgcgatgacg agttccggcg gcagcaggcc ttcggcttcc
     7261 agcgaaatca gctgaccgat cttcatcgcc gcgccgcgca gatgcgcaag ccggtcgacc
     7321 aggcggcgca cggtggcgac cgagaacctg ggggccgagg catctccgtg tctgagtcga
     7381 cgcaagccct gcactgcacc gccgacggcc agctcacccg ccgccaatcc gagctgcgca
     7441 aggcgcccg
//
LOCUS       DATIRT010000383         2523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_850853, whole genome shotgun sequence.
ACCESSION   DATIRT010000383 DATIRT010000000
VERSION     DATIRT010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850853"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(263..450)
                     /gene="ssrS"
                     /locus_tag="VLN25_08665"
     ncRNA           complement(263..450)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VLN25_08665"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            582..1070
                     /locus_tag="VLN25_08670"
     CDS             582..1070
                     /locus_tag="VLN25_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10673.1"
                     /translation="MEGRSFLRRKDREALRERWLLRAGKAFERMFGEANQDRLVTFTE
                     REDRACLLGQELAAFLLEEHAAADRQVRPSEKQALGCPKCEKPGVRVTKRNENLPERE
                     LTTRAGEVQLRREKWWCPKCRINFFPARPQAEVGDRRLQPADCGEGGAAGEQSSVVQG
                     GE"
     gene            1027..2205
                     /locus_tag="VLN25_08675"
     CDS             1027..2205
                     /locus_tag="VLN25_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10674.1"
                     /translation="MRQASKAASFKEASEDLKVLAEVSISPTHLQRLSERVGQEWAQA
                     RDQEVQAFREDKLLCRYEAAPNAAAVMLDGGRVQTRAEDAGRGVHEAGWRETKTACCL
                     SLSSREHAEDPQPEPPSKFLDAERVARLAAEVKSRGRAPRGRAEKGSEKAPAKKRPKK
                     KRRRPASAQRVRTVVATMANSETFGWQMAAEVKRRGLDRARRKACVCDGQPYNWTLFE
                     MHLLPCGFIGILDFVHLLSYLYDAAVAWKKVRVRAWKQYVQWLRWAWLGQVKPLLKSL
                     SQAATELGVPPPDAADADPRNIVNDALRYVQNNRARMDYSRYRCLGLPISSAPVESTI
                     KQINRRIKGTEKFWLNGGAEAMLQLRAAYLSEDDRAATYWARPRPYARAVGAGRLRAA
                     "
     gene            complement(2294..2431)
                     /locus_tag="VLN25_08680"
     CDS             complement(2294..2431)
                     /locus_tag="VLN25_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10675.1"
                     /translation="MQWPATATLIQARLGPTALAIDESNAARLRERLKEIGVEIVQEN
                     G"
BASE COUNT          517 a          712 c          848 g          446 t
ORIGIN      
        1 gaggcaaacg atgttggaat cgggtggttg caagcgtgca aacggctcgc ggtcagacaa
       61 agccaaccca gggcgacgag acagagcctc gtcaaccagt gcctctcagg aatccaacgc
      121 tgaacgatca tggttcgaaa agatgccgcg ccttttctcg attatcccgt agattcgata
      181 cgccatcgaa gggtcgcggg ttcgaatcga atgcagcagc ccggctgctt gcggcggccg
      241 ggctgactgt agcgatcgaa gaaaaacctc cacctgtgcc gttttagact cttttgagaa
      301 cccgcagaag taaaggggcg cctcaggctc ggattttgga tcccactact gggtgggctt
      361 gcaagcaccg aaccatcaaa gtttggctcc cagcgggtac tctatcggtt cgtcaaaagt
      421 aatgtccagt cacgcacacc gcagaggtta atgacaatct acccgatcaa accatcggat
      481 gcaaggggct gggtgcattc ccgttcctgc gccagcgttt ttcgattacc atgatcccca
      541 gtcagctgat cacgatttca gtccctttag gaggtgcggc catggaaggt cgttcatttt
      601 tgcgccggaa ggatcgggag gcacttcgcg agcgatggct gctgcgtgca ggaaaagcgt
      661 ttgagcgaat gttcggtgaa gcgaaccagg accgactggt gacgttcacc gagcgcgagg
      721 acagggcctg tcttttgggc caggagttgg cggcgttcct tttggaggag catgcggcgg
      781 ccgatcgtca ggtgcgaccg tccgagaagc aggcgctggg ttgtcccaaa tgtgaaaagc
      841 ctggcgtgcg ggtcacgaag cggaacgaga atttgcccga acgtgagctg acgacgcggg
      901 caggggaagt ccaactgcgg cgggagaagt ggtggtgtcc gaaatgtcgg atcaattttt
      961 ttcccgctag accacaagct gaagttgggg accgaaggct acagcccgcg gattgtggag
     1021 aagggggtgc ggcaggcgag caaagcagcg tcgttcaagg aggcgagtga agacctcaag
     1081 gtgttggccg aggtgtcgat cagcccgacg cacttgcagc gtttgagcga gcgggtcggc
     1141 caggagtggg cgcaagcacg cgatcaggaa gtgcaggcat ttcgcgagga taagttgctg
     1201 tgccgttacg aggcggcacc caacgcggcg gcggtgatgc tggatggcgg ccgcgtgcag
     1261 acgcgtgccg aggacgccgg ccgtggcgtg cacgaggcgg gctggcgcga gacgaagacg
     1321 gcctgttgtt tgagcctgtc gtcgcgggag catgcagaag atccgcagcc cgagccgccg
     1381 agcaagttcc tggatgccga gcgtgtggca cgcttggccg cggaagtgaa gagtcgcggc
     1441 cgagcgccgc gcggccgtgc ggagaagggc tccgaaaagg caccggccaa gaaacgtccc
     1501 aagaaaaaac ggcgacggcc tgcctccgcc caacgagtgc gcacggtggt ggcgactatg
     1561 gccaacagcg agacgttcgg ttggcagatg gcggccgaag tgaaacgccg tggcctggac
     1621 cgtgcccggc gcaaagcctg cgtctgcgac ggccagccgt acaactggac gctgttcgag
     1681 atgcatctgt tgccttgcgg cttcatcggc atcttggact tcgtgcatct gctctcctac
     1741 ctgtacgacg ccgcggttgc ctggaagaaa gttcgcgttc gtgcatggaa gcagtacgtg
     1801 cagtggttgc gttgggcctg gttggggcag gtcaagccat tgctgaaaag tctgagccag
     1861 gccgcgacgg agttgggagt gccgcctccc gacgcagccg acgccgatcc gcgcaacatc
     1921 gtgaacgacg ccctgcgtta tgtgcaaaac aatcgcgcac gcatggacta ttcacgttat
     1981 cggtgtctgg gtttgccgat cagcagcgcg ccggtggaat cgacgatcaa gcagatcaac
     2041 cgacgcatca aaggcacaga gaaattctgg ctcaacggcg gcgccgaagc gatgctgcaa
     2101 ttgcgtgccg cctacctcag cgaagacgac cgcgctgcca cctactgggc tcggccgcgc
     2161 ccctacgccc gggctgtggg cgccggccgg ctgcgcgcag cgtagcgcag gaacgggaat
     2221 gcacccgctt gccacggcac agcgcgtcaa tggtcgaatc cggggtgcag aactgggaag
     2281 aaatgcggac actctagcca ttttcctgca cgatctccac gccgatttct ttgagacgct
     2341 cccgcagccg cgctgcgttg ctctcgtcga tcgccagcgc cgtcggtccg agccgtgcct
     2401 gaatgagcgt cgcggtcgcg ggccactgca tcaggccgtc ggcaaccgag gcggcgggca
     2461 catgcaagac gagttggcgg cgcaattacg cgggcggcaa ttcaacgccg ctgagcaaca
     2521 ggc
//
LOCUS       DATIRT010000384         3083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1244442, whole genome shotgun sequence.
ACCESSION   DATIRT010000384 DATIRT010000000
VERSION     DATIRT010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244442"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VLN25_08685"
     CDS             complement(<1..786)
                     /locus_tag="VLN25_08685"
                     /inference="COORDINATES: protein motif:HMM:NF015523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HSQ10676.1"
                     /translation="MYEPIVFEMLPAIRPDDWIGAWSAFVQTCRALAKREAWREACAQ
                     IDSVDRRNARSVQAFFAARFDAYRVLARRIEGERELETRDTGLMTGYYEPLLRGSRER
                     SGKFTTPIYKVPPDLLIVDLASLHPELSNLRLRGKLQGRRVVPYPSRADITNGEPLAG
                     NELVWVDDPVEAFFLQVQGSGRVQFNDGNMIRVGYGDQNGHPYRSIGRWLIDQGELTL
                     DQTSMPGIKAWIERNPARAKELLDQNPSFVFFRELPLGDPGAGP"
     gene            complement(1095..1469)
                     /gene="apaG"
                     /locus_tag="VLN25_08690"
     CDS             complement(1095..1469)
                     /gene="apaG"
                     /locus_tag="VLN25_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HSQ10677.1"
                     /translation="MAIYEFSVSVRPQYLDAHSKPEEQHFVFAYTVTIRNTGERTAQL
                     MSRHWIITDANNEIEEVRGDGVVGEQPVLKPGESFEYTSGCPLTTPVGSMRGTYQCVA
                     EDGTAFEATIPEFVLSMPRTLH"
     gene            1611..2315
                     /gene="rpe"
                     /locus_tag="VLN25_08695"
     CDS             1611..2315
                     /gene="rpe"
                     /locus_tag="VLN25_08695"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HSQ10678.1"
                     /translation="MSSGFRIAPSILSADFARLGEEVRAVVAAGADWIHFDVMDNHYV
                     PNLTIGPLVCQAIRPHVEVPIDVHLMVKPVDRIIPDFAKAGANVITFHPEASEHVDRT
                     LALIRDLGCKAGLVFNPATPLAWMDHVMDKIDVVLLMSVNPGFGGQTFIAETLPKLRA
                     VRARVDAYQQATGRPILVEVDGGVKTDNIRMIAEAGADTFVAGSAVFSVRDADGGYRG
                     VIARMRAELAALAHAA"
     gene            2338..3036
                     /locus_tag="VLN25_08700"
     CDS             2338..3036
                     /locus_tag="VLN25_08700"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HSQ10679.1"
                     /translation="MTLIPARAILIDLDGTLLDTVPDLAAAVNAMRADLGRAALPTRT
                     VAAYIGKGAEVLVHRALTGTLDGRADEATFTRGKESFYRHYRRLNGHAALVFPGVLTA
                     LSAFRDGGFRLACVTNKPREFTVDLLDRVGLSEFFDTVISGDDTVEKKPHGAPVLAAC
                     ARLDVPAAEAVMIGDSENDLLSARAAGCRVILVEGGYYEGRSVASLPADAIVETLVDA
                     VALIAPLGQHDDLR"
BASE COUNT          511 a          988 c         1032 g          552 t
ORIGIN      
        1 ggggccggcg cccggatcgc ccagcggcaa ttcgcgaaag aacacaaagc tcggattctg
       61 gtctagcagt tccttggcgc gcgcgggatt gcgttcgatc catgccttga tgccgggcat
      121 cgaggtctgg tcgagcgtca actcgccctg gtcgatcaac cagcggccga tcgatcgata
      181 cgggtgaccg ttctggtcgc cgtagccgac ccggatcatg ttgccatcgt tgaactgcac
      241 tcgacccgag ccctgcacct gcaggaaaaa cgcctcgacc gggtcgtcga cccagacaag
      301 ttcgttgccg gccagcggtt ctccgttggt gatatcggcg cgtgaaggat agggaacgac
      361 gcgccggccc tgcagcttgc cccgcaggcg caggttcgag agttcaggat gcaggctggc
      421 gaggtccacg atcagcaggt cgggcggcac cttgtagatc ggcgtggtga acttgccgct
      481 gcgttcccgg ctgccgcgta acagtggttc gtagtagccc gtcatcagcc ccgtgtcgcg
      541 cgtttccagc tcgcgctcgc cctcgatgcg gcgcgcgagc acgcggtagg cgtcgaagcg
      601 ggcggcgaaa aatgcctgca cgctgcgggc attgcgccgg tccacgctgt cgatctgcgc
      661 gcaggcctcg cgccatgcct cacgcttggc cagggcgcgg catgtctgaa cgaatgcgct
      721 ccaggcaccg atccagtcgt cgggccggat cgcgggcaac atctcgaaca caatcggctc
      781 gtacaccgcg cgggtttcca ccggcgcggc gggcgccggg gtcggacgcg gagttggcgg
      841 aggggtagga gcgggcgcgg gagccggcac agggcgcacg gcgcatccgg ccagcagcac
      901 cgccgcgacg gtcagggcga gccggcgtct agaattcacc gatgcgaaga gactggtcat
      961 gtggctgatt ctgcttgaag ccgcgcttgc gttgggtgcg ctggtgttca tcgtctggtg
     1021 gacgatgttc cagaagccga aacggcgccg gccccctgag cgctaagggt tcagtgcaga
     1081 gatcagtgcg ccgttcagtg cagcgtccgg ggcatcgaca gcacgaactc gggaatcgtc
     1141 gcctcgaagg ccgtgccgtc ctcggccacg cactggtagg tgccacgcat ggaccccacc
     1201 ggcgtcgtca acgggcagcc gctggtgtac tcgaaggatt cgccgggctt cagcactggc
     1261 tgctcgccga ccacgccatc gccacgcact tcctcgatct cgttgttggc gtcggtgatg
     1321 atccagtgcc gcgacatcag ctgcgctgtg cgctcgcccg tgttgcggat ggtcacggtg
     1381 tacgcgaaca cgaagtgctg ctcctcgggt ttcgagtgag catcgaggta ctgcggacgc
     1441 acgctgacgg aaaattcgta gatggccatt gagttcccgg cgagtcgcac gggcaaaggg
     1501 ttgatgggcg cggggttgct agacgcaggt tgatggacgg atcgcatccg gaccgaccct
     1561 tagaatagcc gcgaccggca aagtttccgc atcccaacct tggagtcgcg atgtcctccg
     1621 gctttcgtat agcgccgagc atactgtccg ccgactttgc ccggctcggc gaggaagtcc
     1681 gggcggtggt ggccgcgggc gccgactgga tccacttcga cgtgatggac aaccattacg
     1741 tgccgaacct cacgatcggc ccgctggttt gccaggctat ccgtccgcac gtcgaagttc
     1801 cgatcgacgt gcacctgatg gtcaaaccgg tcgaccggat cattccggac ttcgccaagg
     1861 ccggcgccaa cgtcatcact ttccatcccg aggcgtccga gcatgtcgac cgaacgctcg
     1921 cactgatccg cgacctgggc tgcaaggccg ggctcgtttt caatccggcc acgccgctcg
     1981 cgtggatgga tcacgtaatg gacaagatcg atgtcgtgct gctgatgtcc gtcaacccgg
     2041 gctttggcgg ccagaccttc atcgcggaaa cgctgcccaa gctgcgtgcc gtgcgcgcgc
     2101 gggtcgatgc gtaccagcag gccaccggcc gcccgatcct cgtggaggtc gacggtggag
     2161 tgaagaccga caacatccgc atgatcgccg aggctggagc cgacacattc gtcgccggca
     2221 gtgcagtatt cagtgtgcgc gatgccgacg gcggttaccg cggcgtcatc gcgcgcatgc
     2281 gcgcagaact cgccgcactc gcccacgccg catgacccca atgccggcgc gggccgcatg
     2341 accctgattc cggcgcgggc catcctgatc gacctcgacg gcacgctgct tgatacggtc
     2401 cccgatcttg ccgccgcggt caacgccatg cgggcggatc tgggacgtgc tgcgttgcca
     2461 accaggaccg tggcggccta catcggcaaa ggcgctgagg tgctggtgca ccgggcactg
     2521 acgggcacgc tcgacgggcg tgcggacgag gcgactttca ctcggggcaa ggagtcgttc
     2581 tatcgccact accggcggct caacggtcat gcggcgcttg ttttcccggg cgtgctgacg
     2641 gccttgtcgg catttcgtga cggcgggttc aggctcgcct gcgtcaccaa caagccgcgc
     2701 gagttcacgg tcgacctgct cgaccgcgtg ggactgtccg aattcttcga taccgtgatt
     2761 tccggcgatg acaccgtcga gaagaaacct catggtgcgc cggtgctcgc ggcatgtgcg
     2821 cgcctggacg tgccggccgc ggaggccgtg atgatcggcg attcggaaaa cgacctgctg
     2881 tcagcccggg cagcggggtg tcgggtgatc ctggttgaag gcggctacta cgaagggcgc
     2941 tcggtcgcct cgctgccggc cgatgctata gtcgaaacgc tcgtggacgc ggtcgccctg
     3001 atcgctccac tcggccaaca cgacgatctc cgatgagtca ccgcgaccaa cgcgattccg
     3061 ttttccttgg ggcatggcgc aaa
//
LOCUS       DATIRT010000385         4162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_162072, whole genome shotgun sequence.
ACCESSION   DATIRT010000385 DATIRT010000000
VERSION     DATIRT010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162072"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /locus_tag="VLN25_08705"
     CDS             complement(<1..1214)
                     /locus_tag="VLN25_08705"
                     /inference="COORDINATES: protein motif:HMM:NF021158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HSQ10680.1"
                     /translation="MFLRLTCAVRLLMLLPLGAARAETVTGTATYLQRIALPPSAVFE
                     ASVQEVSGVDAAAEVIGSIRIENPGAPPIAFAIEIDPKRIDERRRYTVRATISVDGKL
                     LLTTDRAYPVLTQGNGREVALLLRSAGGPRPTIVGGPMGLPGARPFRPLPASFGGDLP
                     CADCEALRYRLNLFADRSYFLGTEYVGRKDGNHYDIGTWAVSADVSTLTLKGGREATE
                     LFRVVDARTLRKLMIDGRDIESKLNYALVRTPRFEPVEPRLPMRGMFRFLADAGSFTE
                     CLTGQRWPVASQGQNAVLEREYLKLRKAPGEPLLASVDGEVKLLPRMEGKGVQPMLVV
                     HRLIGVSPGETCGPRLATAGLLDTVWVLTALRGQPVAAKPGPREPSLVLHSEQQRVAG
                     STGCNRLSGSYTL"
     gene            complement(1376..1960)
                     /locus_tag="VLN25_08710"
     CDS             complement(1376..1960)
                     /locus_tag="VLN25_08710"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HSQ10681.1"
                     /translation="MKRMMLALFAALSLLSATAVQAAIYHFEVDLLGANEAPPAASPG
                     FGSAKIDVDTVALTMTLDVVFADLLGVTTASHIHCCNTTPTSPVATQVPSFVGFPLGV
                     SSGTYQHVFDMDLASSYNPSFITNNGGTVDSAFAALVAGMLGGRSYLNIHTNLFPAGE
                     IRGTLIQVPEPASLALLGIALAGLGVLRRRSAAR"
     gene            complement(2280..4097)
                     /locus_tag="VLN25_08715"
     CDS             complement(2280..4097)
                     /locus_tag="VLN25_08715"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSQ10682.1"
                     /translation="MNSPFIDLGLTKELAQVLTGLGYEEPTPIQSQAIPPLLEGRDLL
                     GQAATGTGKTGAFALPLLQRVLAAHRKTAHPAALVLVPTRELAIQVAEALHSYGAKLG
                     AVVLPIYGGAEFGRQAQVLKRGVHVVVATPGRALDHIRRGTLNLSALAAVVLDEADEM
                     LDMGFAEDIELILDAAPAERQTALFSATLPPRIAAIADKHLRDPVRVLIAREKTEKGT
                     LPRVRQTAYIVQRAHKVAALGRVLDLESPALALVFARTRTEVDELAEKLRGRGYQVEA
                     LHGGLSQDQRDRVMKRVRTGSVDLLVATDVAARGLDVEQLSHVVNFNVPASPESYVHR
                     IGRTGRAGREGVAITLAEPREHRLLRNIEHLTKQKIDVATVPTPMDVRARRLDMTRGA
                     LRTALEEGDLDSYRVVTDALASEFDALDVAAAAVKLAHQTLQADAAQDEEHIPSIDPR
                     AAREPWSKGDRPERPTRERRPASDAAPRARPQERGGYEPSPWAMEPKGGKRPARPAPS
                     HVAAGSKRPPTRGGTMVRVYVGAGRSAGLRPADLVGAIANEANISAREIGAIEIEERY
                     SLVEVSDVIAEDAIRALRATSLRGQRVTVRRDQPKRSTK"
BASE COUNT          683 a         1405 c         1410 g          664 t
ORIGIN      
        1 agcgtatagc tgcccgacag gcgattgcag ccggtggagc cggcgacacg ctgctgctcc
       61 gaatgcagca ccaggctggg ctcgcggggc cccggtttag ccgcaaccgg ctggccacgt
      121 aatgcggtca gcacccagac cgtgtcgagc aggcccgccg tcgccagacg cggaccgcag
      181 gtctcgccgg gcgatacgcc gatgaggcga tgaacgacaa gcatcggttg gacgcccttg
      241 ccttccatcc gcggcagaag cttcacttcg ccgtccacgc tggcgagcaa tggctctcca
      301 ggcgccttgc gcagtttcag gtactcgcgc tcgagcaccg cgttctggcc ttgcgacgcg
      361 acgggccaac gctgacccgt cagacattcg gtgaaactgc cggcgtccgc cagaaaacgg
      421 aacatcccgc gcatcggcag gcgcggttcg accggctcga atcgcggggt gcggacgagc
      481 gcgtagttga gttttgactc gatgtcacgg ccatcgatca tcagcttgcg cagggtccgg
      541 gcatcgacca cgcggaagag ttcggtcgcc tcgcggccgc ccttgagcgt cagcgtgctg
      601 acatccgccg agacagccca ggtgccgatg tcgtagtggt tgccgtcctt gcggccgacg
      661 tactcggtgc cgaggaagta ggaccgatcc gcgaacaggt tgaggcgata acgcagcgcc
      721 tcgcagtccg cgcagggcag atctccgccg aaactcgccg gcagcggtcg gaagggccga
      781 gcgccgggca gtcccattgg cccgcccacg atggtcggtc ggggtcctcc ggcggagcgc
      841 aacagcagag cgacttcgcg accgttgcct tgcgtcagca ccgggtaggc gcggtcggtt
      901 gtcagcagca gcttgccgtc gaccgaaatc gtcgcgcgca ccgtgtagcg acggcgttcg
      961 tcgatccgct tcggatcgat ttcgatcgcg aacgctatcg gcggggcgcc gggattctca
     1021 atgcgtatcg agccgatcac ttccgccgcc gcgtcgaccc ccgaaacttc ctggacgctt
     1081 gcctcgaaca cggccgacgg cggcagcgca atgcgctgca ggtaggtcgc cgtgcccgtg
     1141 acggtttcgg cccgggccgc accgagcgga agcaacatca acagccgtac agcacaggtc
     1201 agtctcagga acatgccggc atccggtcgc aggagctcga agacgcgccg cggggtgatt
     1261 ctgcctgcgc ctttgcgcac ggctccagca gaaggcaccc cggcgctggc gacggccggt
     1321 cggatgaaaa aaaaggccca cctcacatgg gcaggcccgt gcagtcgtcg gccgttcagc
     1381 gcgccgccga gcgccgccgc agcacgccga gtccggccag ggcgatgccg agcagtgcca
     1441 gcgatgcagg ttcgggcacc tggatcaagg ttccgcgaat ctcgccggcc ggaaacaggt
     1501 tggtgtggat gttcaggtag gagcgaccgc cgagcatgcc cgccaccagg gcagcgaacg
     1561 cgctgtcgac ggtgccgccg ttgttggtga tgaagctcgg gttgtaggac gaggcgagat
     1621 ccatgtcgaa tacatgctgg tacgtcccac tgctgacgcc gagcggaaag ccgacgaagc
     1681 tcggaacctg cgtggccacc ggtgaggtgg gcgtcgtgtt gcagcagtgg atgtgagagg
     1741 ccgtggtgac gccgagcaga tcggcaaaga ccacatcgag cgtcatggtg agggcaaccg
     1801 tatcgacatc gatctttgca ctgccaaagc cggggcttgc cgccggcgga gcctcgttcg
     1861 cacccagcag atcgacctcg aaatggtaaa tggcggcctg gaccgcggtc gccgagagca
     1921 aagacagcgc tgcaaacagg gccagcatca ttcgcttcat gggacacctc cgcgggaaat
     1981 ttgattgcca tcgaaaccag cgggggaaca ggcaagcaag caacgcacca cgacagaaaa
     2041 tcaatgggct gcgcgtggcg cgacgctaag gtgtcaaggc gatcgacatt ggggcgcgcc
     2101 cgcggtagcg gctatccgga agccgcgtcc gacggcccgc tcgatccgga cgcaaccgcg
     2161 catggcatgc agcatcttct ccaacagcgg cgcaccgcgt gaagccggga gatcgaagcg
     2221 ccgtgggtcg agaagttctg agcggcgccc gctctgcaag gaacctgttt ccgatgtacc
     2281 tacttcgtcg aacgcttcgg ctggtcgcgc cgcacggtga cccgctggcc gcgcaacgat
     2341 gtcgcgcgca gtgcgcggat cgcgtcttcc gcgatgacgt cggacacctc gaccagtgaa
     2401 taacgctcct cgatctcgat cgctccgatc tcgcgggcac tgatgttggc ttcgttggcg
     2461 atcgcgccga ccaggtccgc cggacgcaat cccgcactgc ggccggcgcc cacatacacg
     2521 cgcaccatgg tccctccacg cgtcggcggc cgcttgctac cggcggcgac gtgcgacggg
     2581 gccgggcggg cagggcgctt gccgcctttc ggttccatcg cccaagggga cggctcgtac
     2641 ccgccgcgtt cctgtggccg tgcacgtggc gcggcgtcgg aggcggggcg ccgttcacgt
     2701 gtcggccgct ccggacggtc gcccttggac cacggttcgc gcgcggcgcg cgggtcgatc
     2761 gacgggatgt gctcctcgtc ctgtgccgca tcggcctgca gcgtctggtg cgccagcttc
     2821 accgcggccg ccgcgacatc gagcgcatcg aattcgctgg ccagcgcgtc ggtcaccacg
     2881 cgatagctgt cgaggtcgcc ttcctccagc gccgtgcgca gcgcaccccg cgtcatgtcg
     2941 agccggcgcg cacgcacgtc catcggcgtg ggcaccgtag ccacatcgat cttctgcttc
     3001 gtgaggtgct cgatgttgcg cagcagccgg tgctcgcgcg gctcggccag cgtgattgcc
     3061 acgccttcgc gcccggcgcg gccggtgcgg ccgatgcggt gcacatagct ctccggcgaa
     3121 gccggcacat tgaagttgac gacgtgcgac agttgctcga cgtccagacc gcgcgcggca
     3181 acgtcggtgg ccaccagcag atcgaccgag cccgtgcgca cgcgcttcat cacgcggtcg
     3241 cgctggtcct gcgacaggcc gccgtgcagt gcctcgacct ggtagccgcg gccgcgcaac
     3301 ttctcggcca gttcgtcgac ctcggtacgt gtacgtgcga acacgagagc tagtgcgggc
     3361 gactcgagat ccagcacgcg cccgagcgcc gcgaccttgt gtgcccgctg cacgatgtag
     3421 gcggtctggc gcacgcgcgg cagagtgccc ttctcggtct tctcgcgcgc tatcagcacc
     3481 cgcaccggat cgcgcaggtg cttgtcggcg atggccgcaa tgcgcggcgg cagcgtggcc
     3541 gagaaaagtg ccgtctgccg ttccgcgggc gcggcatcca ggatcagttc gatatcctcg
     3601 gcaaagccca tatcgagcat ctcgtcggcc tcgtccagca cgacggccgc gagcgcgctc
     3661 aggttcagcg tgccgcggcg gatgtgatcg agcgcacgtc ccggtgtggc caccaccacg
     3721 tgcacgccgc gctttagtac ctgcgcctga cgaccaaact ccgcgcctcc atagatcggc
     3781 agcaccaccg cgcccagctt ggcaccgtag ctgtgcagcg cttcggccac ctggattgcg
     3841 agttcgcgtg tcggcaccag caccagcgcg gccgggtgcg cggtcttgcg atgggcggcc
     3901 agcacccgtt gcagcagcgg caacgcgaac gccccggtct tgccagtgcc ggtggcggcc
     3961 tggccgagca ggtcgcggcc ctcgagcagc ggtgggatag cctggctctg gatcggagtc
     4021 ggctcctcat agccgaggcc ggtgaggacc tgcgcgagtt ctttcgtcag gccgaggtcg
     4081 atgaatggac tattcattgg cgcagcgtag cagcgcggca ggtcggctat cgtgtcgcct
     4141 ccgtctcgca caggccctcg cc
//
LOCUS       DATIRT010000386         1897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_359529, whole genome shotgun sequence.
ACCESSION   DATIRT010000386 DATIRT010000000
VERSION     DATIRT010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359529"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="VLN25_08720"
     CDS             complement(<1..1161)
                     /locus_tag="VLN25_08720"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HSQ10683.1"
                     /translation="MPPSTPPIAPGAWLGLLGGGQLGRMFCMAAQSLGYKVCVLDPGR
                     ESPAGAVADDHLCADYLDESALAELARRCRAATTEFENVPAAALAFLAQHCAVSPDAA
                     SVAIAQDRIDEKRFLAASGIQVAPHAVIAQADDLDRLDSRLLPGILKSARLGYDGKGQ
                     ARVATVAEARAAWHAMGGVPCVLEQKLALQLELSVVVARTRDGAVTTFPVAENEHRGG
                     ILAVSIVPARIDEATAQRARSAAIAIATAMNYVGVLCVEFFVLSDGRLLANEIAPRPH
                     NSGHYTIDACVGSQFDQQARVLAGQPLGDSSLLAPAVMLNVLGDVWYEGGRMREPDWP
                     AVLTVPGAKLHLYGKSEARRGRKMGHVTCVAATLPNALVRATEVAHVLGITPP"
     gene            complement(1212..1715)
                     /gene="purE"
                     /locus_tag="VLN25_08725"
     CDS             complement(1212..1715)
                     /gene="purE"
                     /locus_tag="VLN25_08725"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HSQ10684.1"
                     /translation="MNSHGHPTPLVGVVMGSNSDWDVMQHACEVLKEFGVRFEARVVS
                     AHRTPDDMFAYADEARARGLKAIIAGAGGAAHLPGMIASKTVIPVLGVPIPSKYLRGE
                     DSLLSIVQMPRGVPVATFAIGEAGAANAGLFAVAMLATEDAALAMTLAQYRATQTEKA
                     RNMTLPL"
     gene            complement(1787..>1897)
                     /locus_tag="VLN25_08730"
     CDS             complement(1787..>1897)
                     /locus_tag="VLN25_08730"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601066.1"
                     /note="catalyzes the formation of
                     (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-
                     carboxamido)succinate from
                     5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate and
                     L-aspartate in purine biosynthesis; SAICAR synthase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HSQ10685.1"
                     /translation="HWNKAPPPPDLPADIVEKTAAKYREALKRLTGKTLD"
BASE COUNT          304 a          652 c          647 g          294 t
ORIGIN      
        1 cggcggcgtg atgccgagca cgtgcgcgac ctcggtagcg cggaccagcg cgttcggcag
       61 tgtcgccgcc acgcaggtca cgtggcccat cttgcggccg cgtcgcgcct cgctcttgcc
      121 gtacaggtgc agcttggcgc cgggcaccgt gagcactgcg ggccaatccg gctcgcgcat
      181 gcgcccgccc tcataccaga cgtcgccgag gacattcagc atcaccgccg gcgccagcag
      241 actcgaatcg cccagcggct gacccgcgag cacgcgcgcc tgctggtcga actggctgcc
      301 aacacaggcg tcgatcgtgt agtggccgct gttgtgcgga cgcggcgcaa tctcgttggc
      361 gagcaggcgc ccatcggaga gcacgaagaa ctcgacgcac agcacaccga cgtagttcat
      421 ggccgtggca atcgcgatcg ccgcgctgcg cgctcgctgc gcagtcgcct cgtcgatgcg
      481 agccggcacg atcgacaccg ctaggatgcc gccgcggtgc tcgttctcgg ccaccgggaa
      541 ggtcgtcacc gcgccgtctc gggtgcgcgc aacaacaacc gaaagctcga gctgcagcgc
      601 cagcttctgt tcgagcacac atggcactcc gcccatcgca tgccacgcgg cgcgcgcctc
      661 ggccaccgtg gccacccggg cctgcccctt gccgtcgtag cccagccgtg ccgacttgag
      721 gatgccgggc agcaggcgcg agtcgagccg gtcgaggtcg tccgcctgtg cgatgaccgc
      781 atgcggcgcg acctggatac cgcttgccgc taggaagcgc ttctcgtcaa tgcggtcctg
      841 cgcgatcgcc acactcgccg catccggact gacggcacag tgctgcgcca ggaacgccag
      901 tgccgcggcg ggaacgtttt cgaactcggt ggtcgctgca cggcagcggc gcgcaagctc
      961 tgccagcgca gactcgtcga ggtaatcggc acacaggtgg tcgtcggcga cggcaccggc
     1021 cggactttca cggcccggat cgaggacgca gaccttgtag ccgagcgact gcgcagccat
     1081 gcagaacatg cggccgagtt ggccgccgcc gagcagcccg agccaggcgc cgggggcgat
     1141 cggaggggtc gatggcggca tgatgatgga aatgcgggac gggcgcggcg cggtggccgc
     1201 accgcgcgaa actacagcgg caacgtcatg ttgcgcgctt tctcggtctg ggtggcacgg
     1261 tactgcgcaa gcgtcatcgc cagtgcggca tcctcggtcg cgagcattgc cactgcgaaa
     1321 aggcctgcat ttgcggcgcc ggcctcgccg atcgcaaacg tcgccaccgg cacgccgcgc
     1381 ggcatctgca cgatcgacag cagcgagtct tcgccacgca ggtacttgga cgggatcggt
     1441 acgccgagca ccgggatcac ggtcttcgag gcaatcatgc ccggcaggtg cgcggcaccg
     1501 ccggcgccgg caatgatcgc cttcaggccg cgtgcgcgag cctcgtcggc ataggcaaac
     1561 atgtcgtccg gcgtgcggtg ggcggacacg acacgcgcct cgaagcgcac gccgaactcc
     1621 ttcagcacct cgcacgcgtg ctgcatgaca tcccagtcgg agttggagcc catgacgacg
     1681 ccgacgagtg gcgtcggatg accgtggctg ttcattggat gatcgcggcc gagctggctg
     1741 ttttcaggcg aacctcagcg gctcgcgaag gcgccattgt agggaatcag tcgagcgtct
     1801 ttccggtcag gcgcttcaac gcctcgcggt acttcgctgc cgtcttttcg acgatgtctg
     1861 ccggcaggtc gggcggcgga ggcgccttgt tccagtg
//
LOCUS       DATIRT010000387         3071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1931831, whole genome shotgun sequence.
ACCESSION   DATIRT010000387 DATIRT010000000
VERSION     DATIRT010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1931831"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VLN25_08735"
     CDS             complement(<1..726)
                     /locus_tag="VLN25_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HSQ10686.1"
                     /translation="MKLDKAQVLLDEQPISIDVLLEKYAKGDESSVDEVRRRVARGLA
                     QVEQPEARDLWEKRFYDAMVDGFIPGGRVNSAAGTGISATLINCFVQPVGDAISGTRN
                     GVPSIYLALNQAAETMRRGGGVGYDFSPIRPKGALVHGTHSRASGPISYMRVFDKSCE
                     TLESAGARRGAQMGMMRCDHPDIEDFIHAKRDGSLANFNMSVAVTDAFMQAVDSDGEV
                     ELWHVAEPFDKSSGFQRADGSWAY"
     gene            946..1875
                     /locus_tag="VLN25_08740"
     CDS             946..1875
                     /locus_tag="VLN25_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014486084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HSQ10687.1"
                     /translation="MNLQQFRFLREAARHKFSLTDAARALHTSQPGVSKAIIDLEEEL
                     GVPILARHGKRVKGLTAPGQVVLDAAERIMLEVDNLRKLARDYAGSPEGTLRIATTHT
                     QARYVLPQVIQQFAQKYPQVRLKLLQANPPQIASMLVHGQADLGVATESLADAPGLLS
                     IPVYDWQHVAVVKTDHALAKFAATPGKLTLKDLARHPIVTYEHQFAGRGKIDDAFAQV
                     GIEPSIVLEAIDADVIKTYVQIGMGVGILAGVAVDSRRDNLVVLPCGHLFGRNVTRLA
                     VKEGTYLRGFVYSFVEMLVPGWDKRRIEEAFRA"
     gene            1884..2864
                     /locus_tag="VLN25_08745"
     CDS             1884..2864
                     /locus_tag="VLN25_08745"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF005089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HSQ10688.1"
                     /translation="MRVAIVGAGSIGAVIGAKLAATGHEVVLVARGAHLDAIRRDGLR
                     LVDHVGTASGVYRLAASSEPSAFGAQDLVVIGLKAHAIDVMLPRMAPLIAPETTVVPA
                     INGLPWWYFHAEGGALTGTTLRTLDPSGTMFAALDPVHILGCVVHIAAEVRAPGEVHH
                     TGGRRLILGEPDRSLSPRLARVADAFDAAGFATERSPDIRLDVWVKLLGNLSFNPVAA
                     LTGYLMNQIVADDDVLGVIRALMLEGMAVSKHYGYVMPMTPDQRIDLARQLGAAKISM
                     LQDLEQRRPLELAAIVGSVIELADRAGIAVPTIRHVHALTLARARALGIA"
BASE COUNT          524 a         1002 c         1015 g          530 t
ORIGIN      
        1 atacgcccat gagccgtcgg cgcgctggaa gccggacgat ttgtcgaacg gttccgcgac
       61 gtgccagagc tcgacctcgc cgtcgctgtc caccgcctgc atgaaggcgt cggtcactgc
      121 gacggacatg ttgaagttgg cgagcgagcc gtcacgcttg gcgtggatga agtcctcgat
      181 gtccgggtgg tcgcagcgca tcatccccat ctgtgcgccg cgacgggcgc cggcggactc
      241 gagcgtttcg cagctcttgt cgaacacgcg catgtaggag atcgggccgc tggcgcgcga
      301 gtgcgtgccg tgcaccagcg cgcccttcgg ccggatcggc gagaagtcat agccgacgcc
      361 gccgccgcgc cgcatggttt cggcggcctg gttgagcgcg aggtagatcg acggcacgcc
      421 gttgcgcgtg ccgctgatcg catcaccgac gggctgcacg aaacagttga tcagcgtggc
      481 cgagatgccg gtgcccgccg cggaattgac acgcccgccc gggatgaagc catcgaccat
      541 ggcgtcgtag aagcgctttt cccacagatc tcgcgcctca ggctgctcga cctgcgccag
      601 tccgcgtgcg acgcgacggc gaacctcgtc gacactgctt tcgtcaccct tggcatattt
      661 ctcgagcaga acgtcgatcg agatcggctg ctcgtcgagc agtacctgtg ccttgtccag
      721 tttcattgtc gcctcgggga gtcagtccac cgcgtcggaa cgattcacga cgtcgccaga
      781 ctgaccgaaa ttattatatg cagtccgccg cgcaccgcag tccggatgct gatccaccgg
      841 aggtggtaga tcgtgatccc tgttaagcaa tggcatcgga tgaggaagga atcttcctta
      901 taaggcgcaa gtataaaatg cttcaccgat ataacggaga gtcacgtgaa cctgcagcag
      961 tttcgcttcc tgcgcgaggc ggcacgccac aagttcagcc tgacggatgc ggcacgcgcg
     1021 ctgcacacct cgcagcccgg cgtgtcgaaa gcgatcatcg atctcgaaga ggaactcggc
     1081 gttcccatcc tggcgcgcca tggcaagcgg gtaaagggcc tgacggcgcc gggccaggtg
     1141 gtactcgacg ccgccgagcg catcatgctc gaagtcgata atctcaggaa gctggcacgc
     1201 gactacgccg gcagccccga gggtacgctg cgcatcgcga cgacgcacac acaggcgcgc
     1261 tacgtgctcc cgcaggtgat ccagcagttc gcgcagaagt atccgcaggt gcggctcaag
     1321 ctgctgcagg cgaatccgcc gcagatcgcg tcgatgctgg ttcatggcca ggccgacctc
     1381 ggtgtcgcga ccgagtccct ggccgatgcg ccgggattgc tgtcgatccc ggtgtacgac
     1441 tggcagcacg tcgccgtggt caagacggat catgcgctgg caaagttcgc tgccacgccc
     1501 ggcaagctga cgctcaagga cctggcgcgt cacccgatcg tgacctacga gcaccagttc
     1561 gccggtcgcg gcaagatcga cgatgccttc gcccaggtcg ggatcgagcc atccatcgtg
     1621 ctcgaggcga tcgatgccga cgtgatcaag acctatgtgc agatcggcat gggcgtgggc
     1681 atcctcgccg gggtcgcggt cgacagccgc cgcgacaatc tggtggtgct gccctgcggg
     1741 catctgttcg ggcgcaacgt cacgcgcctg gcggtcaagg aaggaacgta cctgcgcggc
     1801 ttcgtttact ccttcgtcga gatgctggtc cccggctggg acaagcgccg catcgaagaa
     1861 gcgttccgcg cgtaggccgg cgcgtgcgcg tcgccatcgt cggcgccggt tcgatcggcg
     1921 cggtcatcgg agccaagctt gcggccaccg ggcacgaggt cgtgctggtg gcacgtggcg
     1981 cgcatctcga tgcgattcgc cgtgacgggt tgcgactggt cgaccatgtg ggcacagcga
     2041 gcggcgtata tcgcctcgcg gccagttccg agccatcggc gttcggcgcg caggacctgg
     2101 tcgtcatcgg cctgaaggcc catgcgatcg acgtgatgct gccgcgaatg gcgccgctga
     2161 tcgcccctga aaccaccgtc gttccggcaa tcaacggcct gccgtggtgg tacttccatg
     2221 ccgaaggcgg cgcgctcacc ggcacgacac tgcgcacgct ggatcccagc ggaacgatgt
     2281 tcgctgcgct cgatccggtg cacatactcg gttgcgtcgt gcacatcgcc gcggaggtga
     2341 gggcgcccgg tgaagttcat cacaccggcg gccggcgact gatcctgggc gaaccggacc
     2401 gctcgctgtc gccacgcctc gcacgagtcg ccgatgcctt cgacgccgcg gggttcgcga
     2461 ccgagcgctc gcccgacatt cgcctcgatg tctgggtgaa gctgctgggc aacctgtcgt
     2521 tcaacccggt cgcggcgctg acgggctacc tgatgaacca gatcgtggcc gacgacgacg
     2581 tgctcggtgt gatacgcgcg ctgatgctcg aaggcatggc cgtgtcgaag cactacggct
     2641 acgtgatgcc gatgacgccg gaccagcgca tcgacctggc acgccagctt ggcgcggcaa
     2701 agatatcgat gctgcaggac ctcgagcagc gacgcccgct cgagttggcc gcgatcgtcg
     2761 gcagcgtgat cgagcttgcc gatcgggccg gcattgcggt gccgacgatt cgccacgtac
     2821 acgcgctcac tctggcgcgt gcgcgcgcgc tcgggatcgc ttaggcgtcg caggtgtacg
     2881 catcggcttc gccgatgcac gtgcacctgc tccggcacgg ctcgcgcgct ccgccgaaac
     2941 gcaacgacaa gcactccacc accgattcag ggcgatatca ggatcggcgt cacagcgtca
     3001 gccgcggcgc cgggtgtcgc gcacgagttc cagtgcggca tcgaagagcg gtgacgcagg
     3061 attcgcgagc t
//
LOCUS       DATIRT010000388         3869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_949679, whole genome shotgun sequence.
ACCESSION   DATIRT010000388 DATIRT010000000
VERSION     DATIRT010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949679"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VLN25_08750"
     CDS             <1..638
                     /locus_tag="VLN25_08750"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HSQ10689.1"
                     /translation="TDAEDLARRAQLNRGEIGALAKADALATLTGHRRRALWTTLGVD
                     ADAPRTAPLAARSRREAAVDLLPPTEGQDIVADYHHTSLTLRRHPLALLRERFNARGY
                     LTAAGIGAARDQQRVRTTGIVTCRQRPSTASGVTFVTIEDETGTVNVVVWNTTGEKYR
                     RALLHSTLMTVYGHVERVTSGVAPIVHLIASRLEDHSPLLGALTMPSRDFH"
     gene            complement(683..1057)
                     /locus_tag="VLN25_08755"
     CDS             complement(683..1057)
                     /locus_tag="VLN25_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10690.1"
                     /translation="MKLRGYSVGTTLFAIGAAVAVPVFGQEAAKHAVTSTSKVLVDNS
                     RVIAGEATFAPGAQNANVARPARVIRGLVGGTLTVIHADGRTEKRTFEPGGVYYFEPD
                     TVANYLRNDGTSEVKLYFVFLK"
     gene            complement(1301..2626)
                     /locus_tag="VLN25_08760"
     CDS             complement(1301..2626)
                     /locus_tag="VLN25_08760"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSQ10691.1"
                     /translation="MSATNAHRIVIIGGGAGGLPLATRLGDRYGRQGSVRVTLVDRNA
                     THVWKPLLHEVAAGRMDADSHDLDYPAIAHWHGFRFSQGSLAGLDRARREITLDAVQS
                     VEGEEILPRRTLGYDTLIICVGSVSNDFGVPGVAQYAISLDMRVDAERFHRRLLAACV
                     RADARAAAGESAQVDIVIIGAGATGVELAAEIRQTTRAHAGYGLEHLDPVRNIRLTLL
                     EAAPRILPPLSDQLASAATELLQRLDVAVQTGQRVTSVDAGGVHTAAGTFFKADLVVW
                     AAGIMAPPVLRTLDGLEVNRANQLVVGPMLQTTQDPDIFAFGDCAAAPWLGHAGNALL
                     PPRAQVAHQQATLLATSIRARLAGRPLPEFHFRDFGSLVSLGELSAVGTLMGRLIGGS
                     MLIQGLIARLMYASLYKMHQVSIHGVMRVAFDTLGRLMRERLEPRVKLH"
     gene            complement(2623..3864)
                     /gene="hutI"
                     /locus_tag="VLN25_08765"
     CDS             complement(2623..3864)
                     /gene="hutI"
                     /locus_tag="VLN25_08765"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HSQ10692.1"
                     /translation="MRCDSLWTGVHLATLADTESASGYGEIRDAALAVRAGRIVWLGR
                     RADLPAELHAEQTIDGGGAWITPGLIDCHTHIVYAGNRSDEFEARLNGANYAEISQRG
                     GGILSTVRATRAATEDALFETSLPRVRALLAEGVTTLEIKSGYGLDLATERKLLRSAR
                     RIGRDLPVTVRTTFLGAHAVPPEFAGRADDYIDEVCERMLPALVGEGLVDAVDAFCES
                     IGFTPAQTERVFRAAAKLGIPVKLHAEQLSDQGGAALVARYGGASADHLEYLSEDGIA
                     AMRQSGTVAVLLPGAFYVLRETRVPPIDALRAAGVMMAVATDCNPGTSPMTSLLLAMN
                     MACTLFRLTPLEALAGTTAVAARALGLQADIGTLEVGKLADFVLWDVERPADLSYAIG
                     FNPCRSVVRAGSAVDAMGQRA"
BASE COUNT          659 a         1358 c         1237 g          615 t
ORIGIN      
        1 tcactgatgc cgaggacctg gcgcgccgcg cgcagctcaa tcgcggcgag atcggcgcgc
       61 tggccaaggc cgatgcgctg gccacgctga ccggccatcg ccgccgggcg ctgtggacca
      121 cgctcggcgt cgatgccgat gctccacgca ccgcaccgct tgccgcgcgc tcgcgccgcg
      181 aagcggcggt cgacctgctg ccgccgaccg aaggccagga catcgtcgcc gactatcacc
      241 acacctcgct gacgctgcgc cgccatccgc tggcgctgct gcgcgagcgc ttcaacgcca
      301 ggggctatct cacggccgcc ggcatcggcg ccgcacgcga ccagcagcgc gtgcgcacca
      361 ccggcatcgt cacctgccgc cagcgcccct ccaccgcgag cggcgtcact ttcgtcacga
      421 tcgaggacga gaccggcacc gtgaacgtcg tcgtctggaa caccaccggc gagaagtacc
      481 gtcgcgcgct gctccactcg acactgatga ccgtctacgg tcacgtggag cgcgtcacga
      541 gcggggtcgc cccgatcgtg cacctgatcg catcccggct ggaagatcac tcgccgctgc
      601 tcggcgcgct cacgatgccg agccgggact ttcactgacc tgggcgccga tcgccatcga
      661 gttgccgccg ccgcattgcc ggtcacttga gaaagacgaa gtagagcttg acttcggacg
      721 tgccgtcatt ccgcaagtaa ttcgccacag tatccggctc gaaatagtag acaccgcctg
      781 gttcgaacgt ccgtttctcc gtgcgaccgt ccgcatggat cacggtcagg gtgccgccga
      841 cgaggccccg gatcacccgc gccggccgtg cgacgttggc gttctgcgcg cctggcgcaa
      901 aggtcgcttc gccggcgatt acgcgggagt tgtccaccag cactttggac gtggaagtca
      961 ccgcgtgctt cgcagcttcc tgcccaaaca ccggaactgc aacagccgcg ccgattgcga
     1021 ataacgttgt gccaaccgag tagcccctta gtttcattgt cccctcccct tgtcattgac
     1081 ttgatgctcc gtggcgaatc gatcgatcgt cgcggagcaa tccagcttgc gcttggcgag
     1141 ctcggctgtc tagggaggga gtttaaagag cgcctgagaa gtcgtttgcc accgattcct
     1201 ggtgggccgt cgctggaatt cgaatcaatg ccgcaccgtt gccttagtgg gttgcagcag
     1261 cgcaccttgg accctggggc cggctgcaat ccgtggcact tcagtgcagc ttgacccgcg
     1321 gctcgaggcg ttcgcgcatc agccggccaa gcgtatcgaa tgccacacgc atcacgccgt
     1381 ggatcgacac ctgatgcatc ttgtagagcg aggcgtacat cagtcgcgcg atcagtccct
     1441 ggatcagcat gctgccgccg atgagccgtc ccatcaacgt accgaccgcg gacagttctc
     1501 cgagcgacac cagcgagccg aagtcgcgga agtgaaactc gggcagcggc cgccctgcga
     1561 gtcgagcgcg gatggatgtc gcgagcagcg tcgcctgctg atgcgcgacc tgggcacgcg
     1621 gcggcaggag ggcattgccc gcatgaccaa gccacggcgc tgcggcgcaa tcgccaaagg
     1681 cgaagatgtc ggggtcctgc gtcgtttgca gcatcggacc gacgaccagc tggttggcgc
     1741 ggttgacctc gagcccgtcg agcgtgcgca ggacgggcgg cgccatgatg cccgccgccc
     1801 agaccaccag gtcggccttg aagaacgtgc cggcggcggt gtgcacgccg ccggcatcga
     1861 cagaagtgac gcgctgaccc gtctgcacgg cgacgtcgag acgctgcagc agttccgtcg
     1921 ccgcggacgc cagttggtcg gacagcggcg gcaggatgcg cggcgcggcc tcgagcagcg
     1981 tgagacggat gttgcgtacc gggtcgaggt gttcgagtcc gtacccggca tgcgcacgcg
     2041 tggtctggcg gatctcggcc gcgagttcga cgccggtggc gccggcgccg atgatgacga
     2101 tgtcgacctg cgccgactca cctgccgccg cccgcgcgtc cgcacgcacg caggccgcca
     2161 acaggcgccg atggaagcgc tcggcgtcga cccgcatgtc gagcgagatc gcgtactgag
     2221 ccacacccgg cactccgaag tcgttgctga cgctgccgac gcaaatgatg agcgtgtcgt
     2281 agcccagcgt gcggcgcggc aggatttcct cgccctcgac cgactgcacc gcatcgagcg
     2341 tgatctcgcg gcgcgcgcga tcgagcccgg ccagcgaccc ctgcgagaac cggaatccat
     2401 gccagtgcgc gatcgccggg tagtcgaggt cgtgcgagtc ggcgtccata cgcccggcgg
     2461 caacttcatg cagcaacggc ttccatacgt gggtggcatt gcggtccacc agcgtcacac
     2521 gcaccgaacc ctggcgtccg tagcgatccc cgagcctggt cgccagcggc aggccgccgg
     2581 caccaccccc gatgatcact atgcgatgcg cattcgtcgc gctcatgcgc gctgccccat
     2641 tgcatccact gcgcttccag cgcgcaccac gctgcgacag ggattgaaac cgatggcata
     2701 ggaaagatcc gccggccgct cgacgtccca cagcacgaag tcagccaact ttccgacctc
     2761 gagggtgccg atatcggctt gcaatccgag tgctcgcgct gcaacggccg tggtgcccgc
     2821 aagcgcttcc agtggcgtca gacggaacag cgtgcaggcc atgttcatag ccagcaacaa
     2881 cgacgtcatt ggcgatgtgc cgggattgca gtctgtggcc accgccatca tcactcctgc
     2941 tgcgcgcaac gcatcgatcg gtggaacgcg ggtctcccgt agcacataga acgcaccggg
     3001 cagcagcacg gctaccgtcc cggactggcg catcgccgcg atgccatcct ccgagaggta
     3061 ctcgagatga tcggcggacg ccccgccgta ccgcgctacg agtgctgcac cgccctggtc
     3121 cgacagctgc tccgcatgca gcttgaccgg aatgcccaac ttcgcggctg cacgaaacac
     3181 gcgctcggtc tgcgcgggcg tgaagccgat cgactcgcaa aatgcgtcca ccgcgtcgac
     3241 cagaccttcg ccgacgagcg ccggcaacat gcgttcgcag acctcgtcga tatagtcatc
     3301 cgcccggcct gcgaactccg gcggcacggc gtgcgccccg aggaacgtgg tgcggacggt
     3361 gaccggcagg tctcgtccga tgcggcgcgc cgagcgcagg agcttccgct cggtcgcgag
     3421 atcgagcccg tagccggact tgatctcgag cgtggtgaca ccctccgcca gcagtgcacg
     3481 cacacgcggc aggctggtct cgaatagtgc gtcctcagtc gccgctcgcg tcgcacgcac
     3541 cgtcgacagg atgccgccgc cgcgctgcga gatctcggcg tagttggcac cgttcagccg
     3601 cgcttcgaac tcatcgctgc ggttgccggc gtagacaatg tgggtgtggc agtcgatcag
     3661 gccgggagtg atccatgctc cgcctccatc gatcgtctgc tccgcatgca gttccgcagg
     3721 caggtcggca cgccggccca accagacaat tcgccctgcg cgcacggcca gggccgcatc
     3781 gcggatctcg ccatagcccg acgcggactc cgtatcggcc aacgtcgcca gatggacacc
     3841 ggtccacagg ctatcgcagc gcacgcgcg
//
LOCUS       DATIRT010000389         2416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1442518, whole genome shotgun sequence.
ACCESSION   DATIRT010000389 DATIRT010000000
VERSION     DATIRT010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442518"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VLN25_08770"
     CDS             <1..228
                     /locus_tag="VLN25_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HSQ10693.1"
                     /translation="GAPVVISSTDPNSGAALLRPMLAQDTGGAIRGPLRFDFFWGFGA
                     DAGQAAGRQKYDVSAWLLVPKGSTPESLLRR"
     gene            complement(253..1020)
                     /locus_tag="VLN25_08775"
     CDS             complement(253..1020)
                     /locus_tag="VLN25_08775"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HSQ10694.1"
                     /translation="MNRWIRRAAVPALALICSVVLAQAPAPKGADAGKSAPVAGSTEA
                     QIAARFAERSGMKADQVFRSQGGLYEVLVRGELYYVDPSVNFVIMGRMFDSRTREDLT
                     QKRLDAALKIDFKSLPFDRAVKTVRGNGSRVLVTFEDPNCPYCKKLWQNMAQLNNVTI
                     YTFLYPILSPDSHEKSKAIWCAKDRAAAWDDYMVQGKAPPAAAADCKNPLEQNVALGR
                     EFGINGTPTIIFADGTRGAGAIPVEAIEQRLAATSKK"
     gene            complement(1110..2285)
                     /locus_tag="VLN25_08780"
     CDS             complement(1110..2285)
                     /locus_tag="VLN25_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HSQ10695.1"
                     /translation="MSDVREVAIAVVGAGVAGMACALGLSQQGLRVALSGPRIVPHAA
                     STAAPYDARIYAVAPATVELLQRLSVWNRIDATRATPVERMRVFGDRGDELTFDAYAA
                     TVERLATILEESELVRVLDAACDYQPGIVRVAAGFDSITTDADGVAVGLGDGASLRCR
                     LLVGADGAQSAVRAAAGMNATVTDYRQIAVVSNFACERPHLNTAWQWFTDEGAVALLP
                     LPGERVSLVWSAPTALAQELAALAPAELARRVTERCGAALGELAPAGPALTFPLRLVV
                     VDRLIAPRVALVGDAAHVVHPLAGQGLNLGLQDVGALLEVVRGREAFRDIGDTVLLRR
                     YERSRAEPIAMMRTATDGLARLFSIDDPLARFVRNTGMAAVDRLPPLKSLLIRQALG"
BASE COUNT          413 a          835 c          770 g          398 t
ORIGIN      
        1 ggtgcgccgg tggtcatttc gtcgaccgat ccgaattcag gcgcggcact gctgcggccg
       61 atgctggcgc aggacaccgg cggcgcgatc cgcggccctc tgcgtttcga cttcttctgg
      121 ggattcggcg ccgatgccgg ccaggccgcc ggccggcaga agtacgacgt cagtgcctgg
      181 ctgctcgtgc cgaagggaag cacgcctgag tcactgctcc ggcgttgacc ttcgcactgg
      241 actcgccgat cgctacttct tgctggtggc cgcgagtcgc tgctcgatcg cctcgaccgg
      301 tatcgctccg gcaccgcgtg ttccatcggc aaagatgatg gtcggcgtgc cgttgatgcc
      361 gaactcgcga ccgagtgcta cgttctgttc gagcgggttc ttgcagtcgg ccgcagccgc
      421 ggggggcgcc tttccctgga ccatgtagtc atcccaggcg gccgcacgat ccttcgcgca
      481 ccagatcgcc ttcgacttct cgtgcgaatc gggcgaaagg atcggataca ggaaggtgta
      541 gatcgtgacg ttgttcagct gcgccatgtt ctgccacagc ttcttgcagt acgggcagtt
      601 cgggtcttcg aacgtcacaa ggacgcgtga accgttgcca cgaacggtct ttaccgcgcg
      661 atcgaacggc aatgacttga agtcgatctt caatgcagcg tcgaggcgct tctgcgtcag
      721 gtcctcgcgc gtgcgcgaat cgaacatgcg gcccatgatg acgaagttga cggacggatc
      781 gacgtaatac agctcgccgc gcaccagcac ttcgtagagc cctccctgac tgcgaaagac
      841 ctgatccgcc ttcatgcccg atctctcggc gaaacgcgcg gcgatctgcg cctcggtgct
      901 gcccgccacc ggagcgctct tgcccgcgtc ggcacctttc ggagccgggg cctgtgccag
      961 gaccactgaa cagatcaggg ccagtgccgg cacggcggcg cgtcgtatcc aacgattcat
     1021 tgacttctcc tgaaagcggc ggcggagtgt agccgcgcgc actgaatgcc tgtcgtgaag
     1081 gtcttcagac cggccactgc cgatcaagtt cagccgagcg cctggcgaat cagcaggctt
     1141 ttgagcggtg gcagccggtc gaccgccgcc attccggtat tgcgcacgaa ccgcgcgagc
     1201 ggatcgtcga tgctgaacag gcgcgccagc ccatcagtgg cggtgcgcat catggcaatc
     1261 ggctcggcgc gactgcgctc gtatcgtcgc aacaggaccg tgtcgccaat gtcccgaaaa
     1321 gcctcgcggc cgcgcaccac ttcgagcaat gcgccgacgt cctgcaggcc gaggttcagg
     1381 ccctgcccgg ccagtggatg caccacgtgg gccgcatcgc cgaccagtgc aacgcgcggc
     1441 gcgatcagcc ggtcgacaac aaccaaccgc aacgggaaag tgagcgccgg gccggcgggg
     1501 gcaagttcac ccaacgccgc cccgcaacgt tcggtgacgc gtcgtgccag ttccgcaggt
     1561 gctagcgcag ccagttcctg cgccaaggcc gtcggcgccg accagaccag ggacacccgc
     1621 tcgcccggca acggcagcaa cgccaccgcg ccttcgtcgg taaaccattg ccaggccgtg
     1681 ttgaggtgcg gccgctcgca ggcaaagttg ctgaccacgg cgatctgccg atagtccgtc
     1741 acggtcgcgt tcatgccggc cgccgcgcgc acagccgact gtgcaccgtc ggcgccgacg
     1801 agcagccggc accgaagcga tgcgccgtcg cccaatccca cggccacccc atccgcgtcc
     1861 gtggtgatcg aatcaaatcc cgctgcaaca cgcacgatgc cgggctggta atcgcaggcg
     1921 gcatccagca cccggaccag ttcgctctcc tcgaggatcg tcgccaggcg ttcgacggtc
     1981 gccgcgtacg catcgaacgt caattcatcg ccacggtctc cgaaaacgcg catgcgctcc
     2041 accggagtgg ctcgcgtcgc gtcgatgcgg ttccagaccg acagccgctg cagcagttcg
     2101 accgtggccg gcgcgaccgc gtagatgcgg gcatcgtacg gcgccgcggt tgacgcagcg
     2161 tgcggaacga tgcgcggccc gctcagcgcg acgcgcagtc cctgctgtga cagccccagc
     2221 gcgcacgcca tgccggccac gccggcaccg acaacggcaa tggcgacctc ccgcacgtcg
     2281 ctcatgccga cggccgctca gggttcggtg tggataccat gctcatggac atcgagtata
     2341 ggagcgcccg ggggacgctc cggtcacgct gaaagctcgc gatgcgacct cgcctgccag
     2401 cgacgacacc gcgagt
//
LOCUS       DATIRT010000390         2121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1932811, whole genome shotgun sequence.
ACCESSION   DATIRT010000390 DATIRT010000000
VERSION     DATIRT010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1932811"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VLN25_08785"
     CDS             complement(<1..881)
                     /locus_tag="VLN25_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSQ10696.1"
                     /translation="MLTAAALSMVPRVALGESPPTGSLITRTIPRSGEKIPAVGMGTW
                     LTFDIGRSAFDLARRRRVMETFYAGGGRLIDSSPMYGRAEAVVGELIAQLGSAAATFS
                     ATKVWTQGRDAGARQMLDSLQLWGLRRFDLMQIHNMVDWETHLATLRQWQTDGRLRYV
                     GITTSHGRRHAALEAALKREAFDFMQVSYSFADRSVEGRLLPLAAERGTAVIINRPFD
                     GGSLVDRLNGFRLPGWAAEIGCTSWAQVCLKWIVSHPAVTCAIPATTDPEHMRENIAA
                     TRGRLPDAALRRTIADHV"
     gene            complement(930..1823)
                     /locus_tag="VLN25_08790"
     CDS             complement(930..1823)
                     /locus_tag="VLN25_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008351457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HSQ10697.1"
                     /translation="MPATRRSLDMTAYAVMLVCTLLWGLQQVTVKVAAAGVSPVMQGG
                     IRSVLATALLLAWSRSRGIALFERDRTMAPGLVAGLLFAAEFFLIYYGLAHTLASRMV
                     IFIYLAPVLTAIGLAWWVPSERLSALQWLGVALAFAGIVAAFADGYASGDRQTLLGDA
                     CGALAAVLWAATTVLIRATRLAGARAEKTLLYQLAVSALLLPLASFALSEPGVMSLDA
                     VTVSMLLYQGVVVAFASYLAWFWLLTRYYAARLSVFAFIAPLAGVAFGVVLLNERLSA
                     SFVAAAVLVAVGIALVNARRG"
     gene            1895..>2121
                     /locus_tag="VLN25_08795"
     CDS             1895..>2121
                     /locus_tag="VLN25_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HSQ10698.1"
                     /translation="MSPDLKPGISYVFDYTVPDSKTVPRLYPEWREFGEMPEVFATGF
                     LVGLMEGTCQLAIKPYLDWPREQSLGTHVSFS"
BASE COUNT          403 a          737 c          686 g          295 t
ORIGIN      
        1 acatgatccg cgatcgtccg ccgcaacgcg gcatccggca agcggccgcg cgtcgccgcg
       61 atgttctcgc gcatgtgctc cgggtcagtc gtcgccggga tggcacaggt caccgcgggg
      121 tgcgagacga tccacttcag gcacacctga gcccagcttg tacagccgat ctctgccgcc
      181 cagcccggca gccggaagcc gttcagccgg tcgaccaacg agccaccgtc gaagggccga
      241 ttgatgatga ccgcggtccc acgctcggcc gccagcggca gcagacgccc ctcgacgctg
      301 cgatcggcaa agctgtagct cacctgcatg aagtcgaaag cctcgcgctt cagcgccgct
      361 tcgagcgccg cgtgacgacg gccatgcgag gtggtgatgc cgacgtagcg gagacgtccg
      421 tcggtctgcc actgccgcag cgtcgccagg tgagtctccc agtcgaccat gttgtggatc
      481 tgcatcagat cgaagcgcct gaggccccag agctgcagcg agtcgagcat ctgccgcgct
      541 cccgcgtcgc ggccctgggt ccagaccttg gtcgccgaga acgtggccgc ggcgctgccc
      601 agctgcgcga tcagttctcc gacgaccgct tcggcacgtc cgtacatcgg gctcgagtcg
      661 atcagccgtc cgccgcctgc gtaaaacgtc tccattaccc ggcgccgccg cgcgagatcg
      721 aacgcactgc ggccaatatc gaaggtgagc cacgtgccca tgccgaccgc aggaatcttc
      781 tcgccactgc gtggaatggt gcgtgttatg agtgagcccg tcggtgggga ctcgccgagc
      841 gccaccctgg ggaccatcga cagtgccgca gcagtcagca agcggcggcg atcgtgcgaa
      901 ggaatgggga aagaaggagc gtgcatcgtt cagcctcgcc gcgcgttgac cagcgcaatg
      961 ccgaccgcga ccagcactgc ggcggcaacg aatgatgcgg acagccgttc attgagcagg
     1021 acgactccga aggccacgcc cgccagcggc gcgatgaacg caaacacgga caggcgcgcg
     1081 gcgtagtagc gcgtcagcag ccagaaccag gcgaggtagc tggcgaaggc cacgacgacg
     1141 ccctggtaca gcagcatcga gaccgtaacg gcgtcgaggc tcatcactcc cggttcggac
     1201 agtgcgaacg atgccagcgg caacagcaac gccgagacgg ccaactggta gagcagcgtc
     1261 ttctcggccc gcgcaccggc caggcgcgtg gcgcggatca gcacggtcgt ggccgcccac
     1321 agtaccgccg ccagcgcgcc acaggcatcg cccagcaggg tctggcgatc gccgctggcg
     1381 tagccgtcgg caaaggccgc cacgatgccg gcaaacgcca gcgccacgcc cagccattgc
     1441 agtgcgctca gacgttcgct cggcacccac caggcgagtc cgatcgcggt cagcaccggc
     1501 gcgagataga tgaagatgac catgcgcgac gccagcgtat gcgccagtcc gtagtagatc
     1561 aggaagaact cggcggcgaa caagagtccg gcgacgagtc ccggcgccat cgtccggtca
     1621 cgctcgaaaa gcgcgatgcc ccggctgcgc gaccacgcga gcagcaacgc cgtggcaagg
     1681 accgagcgga ttccgccctg catgacaggc gacactccgg ccgcagcgac cttgaccgtc
     1741 acctgctgca ggccccacag cagcgtgcag accagcatga cggcatacgc ggtcatgtcc
     1801 agggagcggc gcgtcgcagg catggctgca cgcattctgc cagtgcccca attgccgccg
     1861 cgcgcctaga atccgcccac gctggaggaa gccgatgagc cctgacctga agcccgggat
     1921 cagctatgtc ttcgactaca ccgtgccgga cagcaaaacg gtgccgcgcc tttatcccga
     1981 atggcgtgag ttcggcgaga tgcccgaagt cttcgccacg ggattcctgg tcggcctgat
     2041 ggaaggcacc tgccagctcg cgatcaagcc gtatctcgac tggccgcgcg agcagtcgct
     2101 cggcacgcac gtcagcttct c
//
LOCUS       DATIRT010000391         3860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_853017, whole genome shotgun sequence.
ACCESSION   DATIRT010000391 DATIRT010000000
VERSION     DATIRT010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853017"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..3648
                     /gene="smc"
                     /locus_tag="VLN25_08800"
     CDS             127..3648
                     /gene="smc"
                     /locus_tag="VLN25_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HSQ10699.1"
                     /translation="MRLRQIKLAGFKSFIDPTPIEVPGSLVGVVGPNGCGKSNVIDAV
                     RWVLGESKAAELRGESMQDVIFNGSVNRKPASRASVELVFDNTDGRIGGAWGQYAEIS
                     VKRVLTRDGTSGYYINNQTVRRRDVHDMFLGTGLGPRAYAIIGQGMISRIVEARPEEL
                     RIFLEEAAGISKYKERRRETENRLADTRDNLTRVEDIVRELDSQIGRLEQQAEVAARY
                     RDMVTAHDEQQQLLWLMRKREADVERDKVARDIVARTTALEEHVAELRAAERSREDVR
                     AGHYGASDAVHEAQGALLQVNAEVARLESEIRMIVDGRNRLISQVETLKSTRVRRLDE
                     HGELLARASSLLQAIEDAALRLVESQSACVTHEASVPSLEGAYQAAREAMTAARAQAA
                     LSEQAIEAAGIEQRNVDRQLEVLQQRRERLAEERASIGSPDEARLAGIRAEVVQFESQ
                     LAEHSAAQTTADERVSAGTAERQRQFEARASAQENLTRLEARQGALRQVQANVEADTR
                     LDPWLAEQGLDRLPRLFRKLTVENGWETAFEAVLRERIEGVEVGRLDAVGGLAGSAPP
                     ARVAFYSTTGAITREGGHMPGFGRLSDRVQGHDAALSAVLVDWLANVFVAPDLATAMT
                     ARDQLPVGGQFVVRDGHLVSRYGVRFFAADDDKAGLLARRLEIENLDKEIRAQRLIVE
                     ERTAALARTEAALREAQEQLAASRREAERVRATLHTAQLEAVRLSELIDRVKHRSGQI
                     ETEVAEIEAHENELRATRDDVDGRFQLLDGELSTRQEAVETARLQYEQADQVLRAARD
                     QQRALETARQQVEFALASNRERERDVAHAAEVAAADAERLAVDVSAASEDLARLDDGA
                     ARAGLDGWLGKRAAAEEALRQARSRLDELAQQLRATDERRLTFERELEPRRQKIKELQ
                     LKEQAARLATEQFATQLIEAKADEAALVEKLTPQLKAAGLQAEIARLAQEIEALGAVN
                     LAALEELTTSRERKSFLDAQSADLKAAITTLEDAIRKIDRESREMLQQTFDQVNAHFG
                     QLFPKLFGGGEARLIMTGEEILDAGVQVMAQPPGKRNSSIHLLSGGEKALAATALVFA
                     LFKLNPAPFCLLDEVDAPLDDANTERFCRLVESMTGNTQFLFITHNKIAMEMAQQLIG
                     VTMQEQGVSRIVAVDIEGAVRLTAEAA"
     gene            3655..>3860
                     /locus_tag="VLN25_08805"
     CDS             3655..>3860
                     /locus_tag="VLN25_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10700.1"
                     /translation="MTDLHWALLAIAGVLLLAIWIYSKWQERRVLARLDAAMRHGVVD
                     PLLGARPTSPPASSRRIEPRLDADG"
BASE COUNT          705 a         1234 c         1359 g          562 t
ORIGIN      
        1 aggaagtgcg ctgttattat acgaaggcga tcactcatcc cctcgcattc atcgggccgc
       61 gccttcggtc ggcgcatccc ggtccgcgcc cggggctttt tccagactct tcccactcac
      121 tttcacgtgc gtcttcggca gatcaagctc gccggcttca agtcgttcat cgatcccact
      181 ccgatcgaag ttcccggaag tctcgtcggt gtcgtcggcc ccaacggctg cggcaagtcc
      241 aatgtcatcg atgccgtgcg ctgggtactc ggcgagagca aggccgccga gctgcgcggc
      301 gagtcgatgc aggacgtcat tttcaacggc tcggtcaatc gcaagccggc aagccgcgcg
      361 agcgtcgaac tggtgttcga caacaccgat ggccgcatcg gcggcgcgtg gggacagtac
      421 gcagagattt cggtcaagcg tgtcctgacg cgtgacggca cctcgggcta ctacatcaac
      481 aatcagaccg tgcgtcggcg ggacgtgcac gacatgttcc tcggcacggg actcggcccg
      541 cgcgcctacg caatcatcgg gcagggcatg atctcgcgca tcgtcgaggc acgccctgag
      601 gaactgcgca tcttcctgga ggaagcggcc ggaatcagca agtacaagga acgccggcgc
      661 gaaaccgaga accggttggc cgacacgcgc gacaacctga cgcgcgtcga agacatcgtg
      721 cgcgaactcg acagccagat cgggcggctc gagcagcagg ctgaagtcgc ggcccgctat
      781 cgcgacatgg tgacggcgca cgacgagcag cagcagctgc tgtggctcat gcgcaagcgc
      841 gaggcggacg tcgagcgcga caaggtggcg cgcgacatcg tggcccgaac gacggcgctc
      901 gaagaacatg tcgccgaact ccgcgccgcg gagcggtcgc gggaggatgt gcgcgccggg
      961 cactacggtg ccagcgacgc cgtgcacgaa gcccagggcg cgcttctgca ggtgaatgcc
     1021 gaggtcgcgc ggctcgaatc cgagatccgc atgatcgtcg acggacgcaa ccgcctgatc
     1081 tcgcaggtcg agacgctgaa atcgactcgg gtgcgccggc tggacgagca cggcgagttg
     1141 ctcgcgcggg ccagcagcct gttgcaagcg atcgaggacg ccgcactgcg cctggtcgag
     1201 tcgcagtccg cctgcgtcac gcacgaagcc agcgtgccgt cgctggaggg ggcctaccag
     1261 gcggcgcgcg aggcaatgac cgcggcgcga gcccaggccg cgctgtccga acaggcgatc
     1321 gaggccgccg gcatcgagca gcgcaacgtc gaccgtcagc tggaagtgct gcagcaacgg
     1381 cgcgaacggc tcgccgagga gcgcgcctcc atcggctctc ccgacgaagc acggctggcc
     1441 ggcatccgcg ccgaggtcgt gcagttcgag agtcagctgg ctgagcacag cgcggcgcaa
     1501 acgaccgcgg acgagcgggt cagtgcgggt acggcagagc gacagcggca gttcgaagcg
     1561 cgcgccagcg cacaggagaa cctgacgcgc cttgaagctc gccagggcgc cttgaggcaa
     1621 gtgcaggcga atgtcgaagc cgacacccga ctggacccat ggcttgctga gcaggggctc
     1681 gatcgactgc cgcgcctgtt tcgcaagctg accgtggaaa acggctggga gacggcgttc
     1741 gaagccgtcc tgcgcgagcg catcgaaggc gtggaagtcg gacggcttga tgcggttggc
     1801 ggcctggccg gaagcgctcc accggcgcgc gtggcgttct attcgaccac cggcgccatc
     1861 actcgtgaag gtggacacat gcccggcttc ggccggctgt cggaccgggt gcagggtcat
     1921 gacgccgcgc tgtcggcggt tctggtcgac tggctggcga atgtcttcgt cgcaccggat
     1981 ctcgcgacgg cgatgacggc gcgcgatcag ttgcccgtcg gcgggcaatt cgtggtccgg
     2041 gacgggcatc tcgtctcgcg ctatggcgtg cgcttctttg ccgccgacga tgacaaggcc
     2101 ggtctgctgg cgagacgtct cgaaatcgag aacctcgaca aggaaatccg cgcacaacgg
     2161 ttgatcgtcg aggaacggac cgccgcgctg gcccgcaccg aggccgcgct gcgcgaagcc
     2221 caggagcagc tggcggccag ccggcgcgag gcggagcgtg tgcgcgcgac gctgcatacc
     2281 gcacagctcg aggcggtccg actcagcgaa ctgatcgacc gcgtgaagca ccgcagcggc
     2341 cagatcgaga ccgaggtggc cgagatcgaa gcccacgaga acgagctgcg ggcgacccgc
     2401 gacgacgtcg acggacgctt ccagctgctc gatggtgaac tgtctacgcg gcaggaagcg
     2461 gtggaaaccg cgcgattgca gtacgagcag gcggaccagg tgctgcgcgc agcgcgcgac
     2521 cagcagcgtg cactggagac ggcgcggcag caggtcgagt tcgcgctggc gtcgaaccgt
     2581 gagcgtgagc gcgacgtggc acatgccgca gaagtggctg cggcggatgc cgagcggctt
     2641 gccgtcgacg tgtcggcggc cagcgaggac cttgcacgtc tcgacgatgg cgcggcacgc
     2701 gccggactgg acggctggct ggggaagcgc gcggccgcag aggaggcgct gcggcaggca
     2761 cgcagccggc tcgacgaact ggcccagcag ctgcgcgcga ccgacgaacg ccgattgacg
     2821 ttcgagaggg agctggagcc gcgccggcag aagatcaaag aactgcagct caaggaacag
     2881 gcggcgcgac tcgcgaccga gcagttcgcc acgcagctga tcgaagccaa ggccgatgaa
     2941 gccgcgctgg tggagaagct cacgccacaa ctcaaggccg ccggactgca ggccgagatt
     3001 gcgcggctcg cgcaggagat tgaagcactg ggcgcggtca atctcgccgc gctggaagaa
     3061 ctcacgacct cgcgcgagcg caagtccttc ctcgatgcgc agtcggccga cctcaaggct
     3121 gccatcacga cgctcgagga cgcgatccgc aagatcgacc gcgagtcccg cgaaatgctg
     3181 cagcagacct tcgaccaggt caatgcgcat ttcgggcagc tgttcccgaa gctcttcggc
     3241 gggggcgagg cgcggctcat catgaccggc gaggagatac tcgacgccgg ggtgcaggtg
     3301 atggcgcaac cgccgggcaa gcgcaacagt tcgattcacc tcctgtcggg cggtgagaag
     3361 gctctggcgg ccaccgcgct ggtgttcgcg ctgttcaagc tcaatccggc gccgttctgt
     3421 ctgctggacg aggtcgacgc accgctcgac gacgcgaaca ccgaacgttt ctgccggctt
     3481 gtcgagtcta tgacgggcaa cacgcagttc ctgttcatca cgcacaacaa gattgcgatg
     3541 gaaatggcac agcaactgat cggcgtgacg atgcaggagc agggggtgtc ccgcatcgtg
     3601 gcagtcgaca tcgagggtgc cgttcggctg acggcagaag cggcctaggg caccatgacc
     3661 gacctgcatt gggcgctgct cgctatcgcc ggcgtcctgc tgctggcgat ctggatctac
     3721 agcaagtggc aagagcggcg cgtgttggct cggctggatg ctgccatgcg tcatggggtc
     3781 gtcgatccgc tgctcggcgc gcgtccgacg tcgccgccgg cttcctcgcg gcgtatcgag
     3841 cctcgcctcg acgcggacgg
//
LOCUS       DATIRT010000392         6069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_361290, whole genome shotgun sequence.
ACCESSION   DATIRT010000392 DATIRT010000000
VERSION     DATIRT010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361290"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VLN25_08810"
     CDS             complement(<1..207)
                     /locus_tag="VLN25_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10701.1"
                     /translation="MSPSVAQLALSDFSGGRLGQDRLDTKEARNFEARKRLGAVREEV
                     IGFHNGSLGRHHDGEDFFSAHFVRH"
     gene            complement(306..2819)
                     /locus_tag="VLN25_08815"
     CDS             complement(306..2819)
                     /locus_tag="VLN25_08815"
                     /inference="COORDINATES: protein motif:HMM:NF012535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thaumatin family protein"
                     /protein_id="HSQ10702.1"
                     /translation="MAIGAALLAACGGSSTPTAAPEAPAGLTFEPASSLAIKQGSIHE
                     VVLSLDKSASATGQSVRVATSNLAIADVMPSSCTLSGASEASRRCLLTVRGKALGSVT
                     LSAQSPGLPTIQIPATVDTAVIHGQLAISNASGSFVTTPTASATFPAGATAPFQFPLK
                     AKIVNSSGLSASAGAVIRFSSTTAGVTFTLAQCAVTTLAPECDTVASLPSAAATPIVV
                     SASSSQASSYSNITVNATAAPPANNGTITLSTQSGNQVPNGMKAPLFVQWSNPAVSDT
                     VTVALTIQGTGVSFYSYPAGNNTSATKTQTQSCTLTYLGAGQAGNALSCGLGLVGSAI
                     SGNVTVTATVSSATQQAYSIGSLVLGAVAPEPTVRAITFTNSSTQTIYVGVTGGAATA
                     YVSATKPAVSPGQTTANLKPGAGSLCGPSNPQAACPMGTTCLQGGSSPNTDYTKSPYF
                     CYYDQNAPSNGHTIGAAAGTTTTVLEISGSSLAPNGVIWSGNFYPRTGCDPTTGVCEN
                     ATCKGTAGGLVCGPGTGPSPGTNTLAEVTFQAYPGPDFYDVSIINGANFGTAFGPTNA
                     TVSSSNSYSCGTAGSAQAQNGGYSASGIGLPAASWTMAPTTASFPPGVTLDGDPASYF
                     RLVAPTASPATQCTTAAGTCTDGVNTTCGYAMSDVVTGASFAYDKRYCGKPVAWLTAD
                     AIHGFNATSTNVAPFSLSTSWPNGNGGTVSVGDLQLCINGTYSSYIANLNGSGGPIQP
                     VDLACGGVMWGSTQSTPPLGNPSGNNGLGLTYPNQANPVQTANPNWLSHVLPTITWLK
                     QACPSCYTYPFDDMSSTFTCADSSSNASLNYGVTFSDLN"
     gene            3076..3771
                     /locus_tag="VLN25_08820"
     CDS             3076..3771
                     /locus_tag="VLN25_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HSQ10703.1"
                     /translation="MKTIGLIGGMSWESTVPYYRTINETIKERLGGLHSAKIILYSVD
                     FHDVERLQHSGNWTAAGSLLAATAQAVERAGADVLVLCTNTMHKVAPAIEAAVRIPLL
                     HIADSTAREIVAAGISTVGLLGTRFTMEQGFYKDRRQRNHGVRVLIPDERQREVVHRV
                     IYAELCVGKIVDDSRAEYREIMAQLVRQGAEAIILGCTEITLLVGQADATVPLFDTTS
                     IHARKAAEWALES"
     gene            4244..>6069
                     /locus_tag="VLN25_08825"
     CDS             4244..>6069
                     /locus_tag="VLN25_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HSQ10704.1"
                     /translation="MNAPTELFTAFRESFVSALDEEMALEDFLHYCKSDRMAYASEHE
                     CVLEAIGKPKLVNTADDARLSRIFQNRVIQSWETFSDFHGMEETLMSLYAYFLHAAQG
                     LEERKQVLYLLGPVGGGKSSLAEKIKSLCEQFPIYALKDSMTGKVSPLLESPLSLFPR
                     DKYGAQMEDRYGIPRRALTGIASPWALKRLKEFKGDLSKFRVVRLMPSVLNQIGIVKT
                     EPGDENTQDISSLVGKVDLRMLEQFAQDDPDAYSYSGALCLGSARCVEFVEMFKAPLK
                     VLNPILTATQEGNFKGTEALPALPFTGFIMAHSNEAEWLKFRNNKDNEAFIDRVYLVK
                     VPYCLRVDEEVRIYEKMLASSTLSESPCAPQTLRTLAEFSVLSRLAVPENSSVFSKLR
                     SYNGETLKDVDPRSKSVKEYRDDAGMQEGFSGLSTRWAFKTISAAFNRDPSEIAADPV
                     NLLVVLEDRLAKEDIQEEYKRRLRGHIKEWIVPRFAEFLEKELQMSYLDAYGEYGQNL
                     FDRYVTYADKWLLDEDWRDPDTGMQYSRDALNDELEKIEKPTGISNPKDFRNEVVNFV
                     LRARAHGKNPHWTSYEKLRVVIEKRMFSKTEDILPIISFEAKG"
BASE COUNT         1116 a         1805 c         2012 g         1136 t
ORIGIN      
        1 gtgccggacg aaatgcgcgg agaagaaatc ctcgccatcg tggtgccttc cgagggagcc
       61 gttgtggaac ccgatcacct cttctcgtac tgcgccgagt cgcttgcgcg cttcaaaatt
      121 ccgcgcttcc ttcgtgtcga gacgatcttg cccaagacgc cctccggaaa aatcgctaag
      181 ggccagttgc gcgaccgatg gcgacattcc ctcgcgggct gctatgaccg gtcggtgcag
      241 acatggcttt gatcggacgt gcttctcttg attagctgta cggcgcgcgc aacgcacccg
      301 tgcattcaat tcaggtcgga gaacgtcacc ccgtagttca ggctggcgtt gctcgagctg
      361 tcggcgcacg tgaatgtcga ggacatgtcg tcgaacggat acgtgtaaca gctcggacag
      421 gcctgcttca accaggtgat cgtcggcagg acgtggctca gccagttggg attcgcggtc
      481 tgcacgggat tcgcctggtt cgggtaagtc aggcccaggc cgttgttgcc gctcgggttg
      541 ccgagcggtg gcgtgctctg ggtgctaccc cacatcacgc cgccgcaggc cagatccacc
      601 ggctggatcg gcccgccgct tccattgaga ttggcgatgt agctgctgta cgtgccgttg
      661 atgcaaagtt gcagatcgcc gacgctgacg gtgccgccgt tgccgttggg ccagctggtg
      721 gaaagcgaga acggcgccac attggtgctg gtggcattga agccgtggat cgcgtcggcg
      781 gtgagccagg cgacgggctt accgcagtat cgcttgtcgt aggcgaagct ggcgccggtg
      841 actacatccg acatcgcata gccgcaggtc gtgttgactc cgtcagtgca agtgccggcc
      901 gcggtagtgc actgcgttgc cggcgatgcg gtcggtgcga ccagacggaa gtagctggcg
      961 ggatcgccgt cgagcgtgac accgggcggg aacgacgccg tcgtcggcgc catggtccag
     1021 ctcgccgccg gcaggccgat cccggatgcg ctgtagccgc cgttctgcgc ctgtgcgctt
     1081 cccgcggtgc cgcagctgta agagttggac gaggacacag tcgcattggt cggcccgaac
     1141 gcggtgccga aattggcgcc gttgatgatg gagacgtcgt agaagtctgg accgggatac
     1201 gcctggaatg tcacttcggc cagcgtgttg gttccgggcg atgggccggt gccgggcccg
     1261 cacaccaggc caccggccgt gcctttgcag gttgcgttct cgcagacccc ggtcgtcggg
     1321 tcgcacccgg tccgcggata gaagttcccg gaccagatca ctccgttggg agcgaggctc
     1381 gagcccgaga tctcgagcac ggtcgtggtc gtgcccgcgg ccgctccaat cgtgtggcca
     1441 ttcgagggcg cgttctggtc gtagtagcag aagtacggtg acttcgtgta gtcagtgttc
     1501 ggtgaagagc cgccttggag gcacgtcgtt cccatggggc aggcggcctg cgggttggaa
     1561 ggcccgcaca gcgacccggc accgggcttc agattggccg ttgtctgccc cggtgatact
     1621 gcgggtttgg tcgcactaac gtacgccgtc gccgcgccgc cggttacacc gacgtagatc
     1681 gtttgcgtcg agctgttggt gaacgtgatc gcgcgaacag tcggctccgg cgccaccgcg
     1741 ccaaggacca gcgagccgat gctgtaggcc tgttgcgtgg ccgagctcac cgtggccgtg
     1801 acggtgacgt tgccgctgat cgccgagccc acgagcccga gtccgcagct cagtgcattg
     1861 ccggcctggc cggcgcccag gtacgtcagc gtgcaggact gcgtctgcgt cttggtcgca
     1921 ctcgtgttgt tgcccgccgg gtagctgtag aacgacacgc cggtgccctg gatcgtcagc
     1981 gccaccgtaa cggtgtcgct gacggccggg ttgctccatt gcacgaagag cggcgccttc
     2041 atgccgttgg gcacctggtt gcccgactgc gtgctcaggg tgatggtgcc gttgttcgcc
     2101 ggcggagcag cggtcgcgtt gaccgtgatg ttgctgtagc tgctcgcttg gctgctgctc
     2161 gcgctgacga cgatcggggt cgcggcggcc gatggcagcg acgcgaccgt gtcgcattcg
     2221 ggtgcgagcg ttgtcaccgc gcactgggcc agagtaaacg tgacgccggc cgttgtgctg
     2281 ctgaagcgga tgaccgcgcc ggcggacgca gagagccccg agctgttcac aattttcgct
     2341 ttcagcggaa actggaaggg cgccgttgcg ccagcgggaa aggtcgcgct ggcagtgggt
     2401 gtcgttacga aactgccgga cgcgttgctg atggcgagct ggccgtggat caccgcggtg
     2461 tcgacggtgg cgggaatctg aatcgtgggc aacccgggcg actgggccga gagcgtgacc
     2521 gagccgagcg ccttgccgcg caccgtgagc agacaccgcc gcgatgcctc cgacgcgccg
     2581 gacaaggtgc acgacgacgg catgacatcc gcgatcgcca ggttcgaggt agcgacacgc
     2641 acgctctggc cggtcgcgct ggcggacttg tccagcgaca gcacgacttc atggatgctg
     2701 ccttgcttga tcgcgagcga cgacgcaggc tcgaacgtga ggccggccgg cgcctcgggt
     2761 gcagcagtgg gcgtcgatga gccgccgcag gcggcgagca gcgctgcgcc gatggccatg
     2821 agtgcaatcc gggtcatcgc ggtggggtgc atgtgggttt ctttccggtg tcgtcgctcg
     2881 agacagggga gcgaacaggt tctcgagcgg gggcagttgc aaggagcagc cgcgtacggt
     2941 gcgcaatatg cgcgacgtgg cgtatggaga acgattgaat acggcgggaa ttgcgcagcg
     3001 ttcccggacg tattcgggcc tcacggcggc gcgtggaaca attccgtcga ccacgcatct
     3061 gtgactggat tcgcgatgaa aaccattggc ctgatcggcg gcatgagctg ggagtcgacg
     3121 gtcccgtact accggacgat caacgagaca atcaaggaac gcctaggcgg gctgcattcg
     3181 gcgaaaatca tcctctacag cgttgacttt cacgacgtcg agcggctgca gcattcagga
     3241 aactggaccg cagcaggttc actgttggcc gccactgcgc aggccgtcga gagggccggc
     3301 gcagacgttc tggtgctgtg taccaacaca atgcacaagg tcgcccccgc gatcgaagcg
     3361 gccgtgcgca ttcctttgct gcatatcgcc gattcgaccg cccgggaaat cgtggccgcg
     3421 ggcatctcga ccgtcgggct gcttggtact cgattcacga tggagcaagg cttctacaag
     3481 gatcggcgcc agcgcaacca tggagttcgc gtgctcatcc ccgacgagcg gcaacgcgaa
     3541 gttgttcatc gcgtgatcta cgccgagctg tgtgttggga agatcgtcga cgactcccga
     3601 gccgagtacc gcgaaatcat ggcgcagctg gtcaggcagg gcgccgaagc catcatcctc
     3661 ggatgcacgg agatcacgtt gctggtggga caggccgacg cgacggtgcc gcttttcgac
     3721 acgacgagca ttcatgctcg caaggcggcg gaatgggcac ttgagtcttg atctagttga
     3781 gcaaggtttg gaaagaatcg gcctcgtcgc tcgtcatact cgcctccagc agtgtccgac
     3841 gcccgtcgca gtccttgacc aatccatcga agtcacgcgt cattcgcgct tgtttcatct
     3901 ctttggaggc ctgcgtgaaa gctcgagccg cgacgtaatg tgcaacgcag cggaaccgaa
     3961 ggggccagag cgggcgagcc gcggcgcact tcggaagttc ggatgaggct gcgcctcccc
     4021 tttgaatgtg cgatttcctg cataagtacg cgcaagcacg acaacttccg cgaccgcgac
     4081 tgaggaatcg ggcacaccac cgatgaatcg agcgacgctg gcgagcactc gacagtggcg
     4141 ggcatttact cttcttttgc tcgtatcacg acaagcgctt tgtcctctac cctgagttcg
     4201 tcgcgtttgc ttcatcgacg gcccacgggg caaggaggtc tggatgaacg cgcccaccga
     4261 actgtttacc gcgttccgcg aatccttcgt cagtgcactg gacgaagaga tggcgctgga
     4321 agacttcctg cactactgca agtccgaccg gatggcctat gcgagcgagc acgagtgcgt
     4381 tctcgaggcg atcggcaagc ccaagctcgt caacacggcc gatgacgcgc ggctgtcgcg
     4441 catattccag aaccgcgtga ttcagagctg ggaaacgttc tctgactttc acggcatgga
     4501 agagacgctg atgtcgctgt atgcgtactt cctgcacgcg gcacaggggc tcgaagagcg
     4561 caagcaggtg ctgtatctgc tcggccccgt cggaggcggc aaatcgtcgt tggcagaaaa
     4621 gatcaagtcg ctgtgcgagc agtttcccat ctatgcgctg aaagactcga tgacgggcaa
     4681 ggtctcgcca ctgctcgagt caccgctgag cctcttcccg cgcgacaagt acggcgcaca
     4741 gatggaagac cgctatggca ttccgcgtcg agcgctgacg ggcatcgcca gcccatgggc
     4801 gctgaagcgc ctgaaggaat tcaagggcga tctgtcgaag tttcgggtgg tgcgtctcat
     4861 gccatcggtg ctcaaccaga tcggcatcgt caagaccgag ccgggcgacg aaaacacgca
     4921 ggacatctcg tcgctggtcg gaaaggtcga tctgcgcatg ctggagcagt tcgcgcagga
     4981 cgacccggat gcgtacagct attcgggtgc gctgtgcctg ggctcggcgc gctgcgtcga
     5041 attcgtcgag atgttcaagg caccactgaa ggtgctcaac ccgatcctga cagcgaccca
     5101 ggaaggtaat ttcaagggca ccgaagcgct gccggcattg ccgttcaccg gcttcatcat
     5161 ggcgcactcg aatgaagccg agtggctgaa gttccgcaac aacaaagaca acgaggcatt
     5221 catcgaccgc gtgtatctgg tcaaggtgcc ctactgcctg cgtgtcgacg aagaagttcg
     5281 aatctacgaa aagatgctgg cgtcctcgac gctgtcagag tcaccgtgcg cgccacagac
     5341 gctgcgcacg ctggccgagt tctccgtgct ctcgcggctg gccgtgccgg aaaactcgtc
     5401 cgtcttcagc aagctgcgtt cgtacaacgg cgagacgctc aaggacgttg atccgcgcag
     5461 caagagcgtg aaggagtatc gcgacgacgc cggcatgcag gaaggcttct cgggcctttc
     5521 gacgcgctgg gcgttcaaga cgatctcggc cgctttcaat cgcgacccga gcgagatcgc
     5581 ggccgacccc gtcaatctgc tggtcgtgct cgaggatcgc ctggcgaagg aagacatcca
     5641 ggaggaatac aagcggcggc tccgcggtca catcaaggaa tggatcgtgc cgcgcttcgc
     5701 cgagttcctc gagaaggaac ttcagatgtc gtacctcgat gcgtacggcg agtacggtca
     5761 gaacctgttc gaccgctacg tgacctatgc ggacaaatgg ctgctcgatg aagattggcg
     5821 cgatccggac actggcatgc agtattcgcg cgatgccctg aacgacgagc tcgagaagat
     5881 cgagaagccg accggcatct cgaacccgaa ggactttcgc aacgaagtcg tcaacttcgt
     5941 gctgcgggcg cgcgcgcacg gcaagaaccc gcattggacc agttacgaaa agctacgcgt
     6001 ggtgatcgaa aagcgcatgt tcagcaagac cgaggacatc ctgccgatca tctccttcga
     6061 ggccaaggg
//
LOCUS       DATIRT010000393         2635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_853523, whole genome shotgun sequence.
ACCESSION   DATIRT010000393 DATIRT010000000
VERSION     DATIRT010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853523"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VLN25_08830"
     CDS             complement(<1..308)
                     /locus_tag="VLN25_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10705.1"
                     /translation="MKALLDLFKQVQQQEQFDSIKIGIASPDKIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPIKDYECLCGKYKRLKHRGVICEKCGVEVTLAKVRRERM
                     G"
     gene            complement(341..>2635)
                     /gene="rpoB"
                     /locus_tag="VLN25_08835"
     CDS             complement(341..>2635)
                     /gene="rpoB"
                     /locus_tag="VLN25_08835"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HSQ10706.1"
                     /translation="HVTTEISYLSAIEEGKYVIAQANAPLDKTGKFMDELVSCRENGE
                     FVLMSPERVQYMDIAPSQIVSVAASLIPFLEHDDANRALMGSNMQRQAVPCLRPEKPF
                     VGTGVERTVAVDSGTTVQALRGGLVDYVDANRIVVRVHENENVAGEVGVDIYNLTKYT
                     RSNQNTNINQTPIVKEGDVIAKGDVIADGASTDLGELALGQNMLVAFMPWNGYNFEDS
                     ILISERVVADDRYTSIHIEELNVVARDTKLGPEEITRDISNLSENQLARLDDSGIVYI
                     GAEVNAGDVLVGKVTPKGETQLTPEEKLLRAIFGEKASDVKDTSLRVPSGMSGTVIDV
                     QVFTREGIVRDKRAQQIIDDALKRYRQDLNDQLRIVEGDAFQRIEKMLLKKDAIGGPR
                     KITKGTVITKDYLESLEKYHWFEIRLADDEAAAQLEGLKDSIELKRKQFDQAFEDKKK
                     KLTQGDELPPGVLKMVKVYLAVKRRLQPGDKMAGRHGNKGVVSRIVPVEDMPYMADGT
                     PVDIVLNPLGVPSRMNVGQILETHLGWAAKGLGQRIGDMLATAKSAQVRQLLKEIYNR
                     SGKPEDLDHLSDDEVTQLAEHLRDGVPFATPVFDGAAEKEIHAMLDLAYPEEVAKRLQ
                     LTATKTQTRLFDGRTGDAFERTVTVGYMHMLKLHHLVDDKMHARSTGPYSLVTQQPLG
                     GKAQFGGQRFGEMEVWALEAYGAAYVLQEMLTVKSDDVQGRTKVYENIVKGDHVIDAG
                     MPESFNVLVKEIRSLGIDMDLERN"
BASE COUNT          418 a          856 c          797 g          564 t
ORIGIN      
        1 cccatgcgct cgcgccggac cttggcgagc gtcacctcga cgccgcactt ctcgcagatc
       61 acgccacggt gcttcaggcg cttgtacttg ccgcacaggc actcgtagtc cttgatcggc
      121 ccgaagatct tggcgcagaa cagaccatcg cgctccggct tgaacgtgcg gtagttgatc
      181 gtctcgggct tcttgacttc gccgaaggac cacgagcgga tcttgtcggg agaggcgatt
      241 ccgatcttga tggaatcgaa ctgctcctgc tgctgtacct gcttgaatag atcgagtagc
      301 gctttcatgc gtacctctca gtaatctcgt cgatgagcgg ttagttgcgc tcgagatcca
      361 tgtcgatacc caacgaccgg atttccttga ccagcacgtt gaacgactcc ggcatgccgg
      421 cgtcgatcac gtgatcgccc ttgacgatgt tctcgtagac cttggtgcgg ccctgcacgt
      481 cgtcgctctt gacggtgagc atctcctgca gcacatacgc ggcgccgtag gcctcaagcg
      541 cccacacttc catctcgccg aagcgctggc cgccgaactg cgccttgccg cccagcggct
      601 gctgggtgac gagcgagtac gggccagtcg accgcgcgtg catcttgtcg tcgaccaggt
      661 ggtgcagctt cagcatgtgc atgtagccga ccgtcaccgt gcgctcgaat gcgtcgccgg
      721 tacgtccgtc gaaaagccgc gtctgcgtct tcgttgcggt cagctgcagg cgctttgcca
      781 cttcctccgg ataggcgagg tcgagcatcg cgtggatttc cttctccgcc gcaccgtcga
      841 acaccggcgt ggcgaacgga actccgtcgc gcaggtgttc ggcgagctgc gtgacctcat
      901 cgtcactcaa gtgatcgaga tcctccggct tgccggagcg gttgtagatc tccttcagca
      961 gctggcgcac ctgggcagac ttcgccgtgg ccagcatgtc accgatgcgc tggcccaggc
     1021 ctttggcggc ccaacccagg tgcgtctcga ggatctggcc gacgttcatc cgggacggga
     1081 caccgagcgg gttcagcacg atgtcgaccg gcgtcccatc ggccatgtac ggcatgtcct
     1141 cgacgggaac gatgcgcgac accacgccct tgttgccgtg ccggcccgcc atcttgtcgc
     1201 ctggctgcag gcgccgcttg accgccaggt agaccttgac catcttcagc acgcctggcg
     1261 gcagttcgtc gccctgcgtc agcttcttct tcttgtcctc gaaggcctgg tcgaactgct
     1321 tgcgcttcag ctcgatcgaa tccttcagtc cctcgagctg cgccgcggct tcgtcatcgg
     1381 ccaggcggat ctcgaaccag tgatatttct cgagcgactc gagatagtcc ttggtgatga
     1441 ccgtgccctt ggtgatcttg cgcgggccac cgatggcatc cttcttcagc agcatcttct
     1501 cgatgcgctg gaaggcgtcg ccctcgacga tgcgcagctg gtcgttcagg tcctggcggt
     1561 agcgcttgag cgcgtcatcg atgatctgct gggcgcgctt gtcgcgcacg atgccttcgc
     1621 gcgtgaagac ctggacgtcg atcacggtgc cgctcatgcc cgacggcacg cgcagcgacg
     1681 tgtccttcac gtccgaggct ttctcgccga agatcgcacg cagcaacttt tcttccggcg
     1741 tcagctgagt ctcgcccttg ggagtgacct tgccgacgag cacgtcaccc gcattgacct
     1801 cggcaccgat gtaaacgatg ccggagtcat cgaggcgcgc cagctggttc tcggacagat
     1861 tgctgatgtc gcgggtgatc tcctctggcc cgagcttggt atcgcgcgcg acgacgttca
     1921 gttcctcgat atggatcgag gtatagcggt cgtccgcgac cacgcgctcg gagatgagga
     1981 tcgagtcctc gaagttgtac ccgttccacg gcatgaacgc gaccagcatg ttctggccca
     2041 gcgccagttc gcccagatcg gtcgatgcgc cgtccgcgat cacgtcgcct ttggcgatga
     2101 catcgccctc cttgacgatc ggcgtctggt tgatgttcgt gttctggttc gagcgcgtgt
     2161 acttggtcag gttgtaaatg tcgacgccga cttcacccgc cacgttctcg ttttcgtgca
     2221 cgcgcacgac gatgcggttg gcatcgacgt agtcgaccag tccgccgcgc agtgcctgca
     2281 cggttgtgcc cgagtcgacc gcgaccgtgc gctccacacc ggtaccgacg aacggcttct
     2341 ccggacgcag gcagggcacg gcctggcgct gcatgttcga gcccatcagc gcgcggttgg
     2401 cgtcgtcgtg ctcgaggaac gggatcaggg aggccgccac ggaaacaatc tgcgacggtg
     2461 cgatgtccat gtactgcaca cgctccggcg acatcagcac gaattcaccg ttctcgcggc
     2521 agctcacgag ctcgtccatg aacttgcccg tcttgtcgag cggggcattg gcctgcgcga
     2581 tcacgtactt gccttcctcg atcgcggaca gatacgagat ctcggtggtg acgtg
//
LOCUS       DATIRT010000394         3862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1443884, whole genome shotgun sequence.
ACCESSION   DATIRT010000394 DATIRT010000000
VERSION     DATIRT010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443884"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VLN25_08840"
     CDS             <1..227
                     /locus_tag="VLN25_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10707.1"
                     /translation="IAREAFRHAVSGIERVAAGSGVGVDISVRLKRPVALPDDPQRPA
                     LTHWRRALRIEADELLASITSRIALVNVHA"
     gene            214..513
                     /gene="yidD"
                     /locus_tag="VLN25_08845"
     CDS             214..513
                     /gene="yidD"
                     /locus_tag="VLN25_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HSQ10708.1"
                     /translation="MSMPDGFFARLLVRLIGAYQYLLSPWVGNSCRYWPTCSEYAREA
                     IERHGAGRGSYLAARRLARCHPYGRGGVDPVPGVFSWRCRCAGVGEASNAGETAT"
     gene            596..2359
                     /gene="yidC"
                     /locus_tag="VLN25_08850"
     CDS             596..2359
                     /gene="yidC"
                     /locus_tag="VLN25_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HSQ10709.1"
                     /translation="MGTDFQRTVLWVFFGMSLFLLWDRWLVHTGKPSLFGTAPAPQVS
                     APAAAPPSAPVAPSPAAPAAATNAAIPTAAAPVPAASPTAVPTSPVATASRQPPVVIQ
                     TDVMRATIDPEGAVLSRVELLKEKVAPDWTASGLLGLITGKTSDSNAPVVLLDVSPQR
                     VYVAQTGIVGADASLPNHRSPFTVVDGPRTLAQGKDAVDVTLVSESGGVKVAKTYSFH
                     RGRYDVTVKHDVTNVGEAPVTPSLYQQIMRDGNKPEGESALYYTYTGPAVFTEQGKFK
                     KIEFSAIEKNKPELPKPTKDGWVGMIQHYFVTAFVPQEGTQREYYARMVDKNLYSIGM
                     LQPLSVLAPQASTTATTQLYVGPQDQHALAKLAPGLDLVVDYGWLTFLAKPIYWLLEF
                     LHRLVGNWGWAIVLLTVLIKAAFYPLSAASYKSMARMKEVTPRMMKLREQFGDDKQKL
                     NTAMMELYKNEKINPLGGCLPILVQIPVFISLYWVLLASVEMRNAPWILWVTDLATPD
                     PWFILPVLMMATMWIQYKLNPTPPDPVQAKVMMFMPLIFGVMFFFFPAGLVLYWLTNN
                     VLSIAQQWYVTKQIEKAKKPG"
     gene            complement(2393..2710)
                     /locus_tag="VLN25_08855"
     CDS             complement(2393..2710)
                     /locus_tag="VLN25_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10710.1"
                     /translation="MNDDPLLKFISPLAGVVLAVLLSVMVSATVAGQIGDDYATRAWI
                     YTISVLWVVVGAVVVFMLTHRGESKTLSIGRVLLWTASIWLWPVFALRALQRRRPPNE
                     PPT"
     gene            2842..>3862
                     /gene="mnmE"
                     /locus_tag="VLN25_08860"
     CDS             2842..>3862
                     /gene="mnmE"
                     /locus_tag="VLN25_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HSQ10711.1"
                     /translation="MNSDKDPIVAIATAPGRGGIGIVRVSGSDLGPVIEGVLGGRTPS
                     PRHATVCAFIDGRGVAIDQGIALHFPAPHSYTGEDVFELQGHGGPVVLRLLVARCLEA
                     GRAIGLRVAEPGEFTRRAFLNDRLDLAQAEAVADLIDASTEAAARSAARSLTGEFSRA
                     IHGLTGQLTELRALVEATLDFPEEEVDFLEKADAAGRLQRVRAALDEVMHRAQQGALL
                     RDGLHVVLVGAPNVGKSSLLNALAGEDVAIVTAVPGTTRDRIARQIQIGGLPLDIIDT
                     AGVRETTDEVETLGIARTLAEVERADVVLHLVDSLRPSADEDVLARVTARAGRGVPLL
                     TVVNKI"
BASE COUNT          636 a         1270 c         1300 g          656 t
ORIGIN      
        1 gcatcgcgcg cgaagcgttt cgccacgcag tttcgggaat cgagcgcgtc gccgccggtt
       61 cgggtgtggg cgtggacatc agcgtacggc tcaagcgtcc cgtcgcgctg ccggatgatc
      121 cgcagcggcc cgcgttaacc cattggcgcc gcgcgctgcg catcgaggcc gatgaactgc
      181 tcgcgtcgat cacctcccgc atcgccctgg tcaatgtcca tgcctgacgg gttcttcgcg
      241 cgcctgctgg tgcgactgat cggcgcctac cagtatctgt tgtcgccctg ggtcggcaat
      301 agctgccgct attggccaac gtgctccgag tacgcgcgcg aggccatcga gcgccatggc
      361 gccggacgcg gcagctacct tgccgcgcgg cgtctcgctc gctgccatcc gtacgggcgc
      421 ggcggagtcg atccagtgcc cggcgtgttt tcctggcgtt gtcgctgcgc cggggtcggc
      481 gaggccagca atgcggggga aacggcgacc tgagcgcatg cactgtgcgc cgccacgcct
      541 gagcttcatc gagcgaacct tttcaacttc ttcttagccg gggacacctc ccgtcatggg
      601 tactgatttc caacgcaccg tcctgtgggt cttcttcggc atgtcgctgt tcctgttatg
      661 ggaccgctgg ctggtccaca ccggcaagcc gtcgctgttc gggaccgcgc cggcgccaca
      721 agtgagcgcg cccgcggcag ctccgccgag cgcgcctgtc gccccttccc cggccgcgcc
      781 cgcggccgcc acaaacgccg cgataccgac tgcagcggcg ccggttccgg cggccagccc
      841 gacggcggtc ccgacttcgc ctgtcgccac cgcgtccagg caacccccgg tggtcatcca
      901 aaccgacgtc atgcgcgcca ccattgatcc ggagggcgca gtgctgtcgc gcgtggagct
      961 attgaaggaa aaggtcgcgc ctgactggac tgcgagcggg ctgctggggc tgatcactgg
     1021 caagacgtcc gacagcaacg cgccggtagt cctgctcgac gtcagtccgc agcgagtcta
     1081 tgtcgcacag accggcatcg tcggcgcgga tgcgtctctg cccaaccatc gctcgccgtt
     1141 cacggtcgtc gacggcccgc gcacgctggc gcagggcaag gatgccgttg atgtgacgct
     1201 ggtgtcggag tcgggcggcg tgaaggtggc caagacgtac tcgttccatc gcggccggta
     1261 tgacgtcacc gtcaagcacg atgtgaccaa cgtcggcgag gcgccggtca cgccgtcgct
     1321 gtaccagcag atcatgcgcg acggcaacaa gccggaaggt gagtcggctc tttattacac
     1381 ctacaccggg ccggccgtct tcacggagca gggcaagttc aagaagatcg agttttcggc
     1441 gatcgagaag aacaagcccg agctgccgaa gccgaccaag gacggttggg tcggcatgat
     1501 ccagcactac tttgtcaccg cattcgtgcc gcaggagggc acacagcgcg agtactacgc
     1561 gcgcatggtc gacaagaacc tgtactcgat cggaatgctg cagccgctgt cggtgctggc
     1621 gccgcaggca agcacgacgg ccaccacaca gctttatgtc ggcccgcagg accagcacgc
     1681 actggcgaag cttgctcccg ggctggatct tgtcgtcgac tacggctggc tcacgttcct
     1741 ggccaagccg atctactggc tgctcgagtt cctgcaccgc ctcgtcggca actggggctg
     1801 ggcgatcgtg ctgctgacgg tgctgatcaa ggctgctttt tatccgctct ctgccgccag
     1861 ctacaagtcg atggcgcgca tgaaggaagt gacgccgcgg atgatgaaac tgcgcgagca
     1921 gttcggcgac gacaagcaga agctcaacac ggcaatgatg gagctctaca agaacgagaa
     1981 gatcaacccg ctcggtggct gcctgccgat cctggtgcag atcccggtgt tcatctcgct
     2041 ctactgggtg ctgctggcga gcgtggagat gcgcaatgct ccatggatcc tgtgggtcac
     2101 ggacctggcc acgccggatc cgtggttcat cctgccggtg ttgatgatgg ccaccatgtg
     2161 gatccagtac aagctgaatc cgacgccgcc cgatccggta caggccaagg tcatgatgtt
     2221 catgccgctg atattcggcg tgatgttctt cttcttcccg gccggcctcg tgctgtactg
     2281 gctgaccaac aacgtcctgt cgatcgccca gcagtggtac gtgaccaagc agatcgagaa
     2341 ggcgaagaag ccgggttgat gcgcccacgc ggcgggcgcg tcaggccatc gctcaggttg
     2401 gcggctcgtt cgggggacgg cgccgctgca atgcgcgcag cgcaaacacc ggccacagcc
     2461 agatgctggc ggtccacaac aggacgcggc cgatcgacag cgtcttgctc tcgccgcggt
     2521 gggtgagcat gaaaacgacg acggcaccca ccacgaccca cagcacggag atcgtgtaga
     2581 tccacgcgcg cgtggcgtag tcgtcgccga tctgcccggc gacggtggcc gagaccatca
     2641 ccgacagcag caccgcgagc acaacccccg ccagcggcga gatgaacttc agcagcggat
     2701 cgtcgttcat gggcacagtg tagggatgcc gggcaagacg gccacggcgg gagtgccctc
     2761 ggttggtcgg caagcgggtg gaggggcagg tatgcgtcag tccagaatgc ggtctcgcct
     2821 ctgccccagc gatccagtga catgaattcc gacaaggacc ctatcgtggc gatcgccacc
     2881 gcgcccggcc gcgggggcat cggcatcgtg cgcgtgtcgg gctcggacct gggcccggtc
     2941 atcgagggcg tgctcggtgg acgcaccccg tcgccccggc acgcgacggt ctgcgccttc
     3001 atcgacggtc gtggcgtggc gatcgatcag ggcatcgccc tgcattttcc ggcgccgcat
     3061 tcgtacaccg gcgaggacgt gttcgagttg cagggccatg gcggaccggt ggtgctgcgc
     3121 ctgctggtgg cgcgctgcct cgaggccggc cgcgcgatcg gattacgcgt cgcggagccc
     3181 ggcgagttca cgcggcgtgc gttcctgaac gatcggctcg acctggcgca ggccgaagcg
     3241 gtggccgacc tcatcgatgc gtcgaccgag gccgcggcgc gctcggcggc gcgctcgctc
     3301 accggagaat tctcgcgggc gatccacgga ctgaccggcc agctcaccga actgcgtgca
     3361 ttggtcgagg ccacgctcga cttccccgaa gaggaggtcg acttccttga gaaagccgat
     3421 gcggccgggc ggctgcagcg cgtgcgcgcg gcgctcgatg aggtgatgca tcgggcacag
     3481 cagggcgcac tgctgcgcga cggcctgcat gtcgtgctgg tgggcgcgcc gaacgtcggc
     3541 aagtcaagcc tgctaaatgc gctggccggc gaggatgtcg cgatcgtcac ggccgtgccc
     3601 ggcacgacgc gagaccgcat cgcgcggcag attcagatcg gcggcctgcc gctcgacatc
     3661 atcgacacgg ctggcgtgcg cgagacgacc gacgaagtcg agacgctggg catcgcgcgc
     3721 acgctggctg aagtggagcg tgccgatgtc gtcctgcacc tggtggattc gctgcgaccg
     3781 agtgccgacg aggacgtcct tgcgcgcgtc accgcgcgtg ccgggcgtgg cgtcccgctg
     3841 ctcaccgtgg tcaacaaaat cg
//
LOCUS       DATIRT010000395         4226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_657155, whole genome shotgun sequence.
ACCESSION   DATIRT010000395 DATIRT010000000
VERSION     DATIRT010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657155"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VLN25_08865"
     CDS             <1..843
                     /locus_tag="VLN25_08865"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HSQ10712.1"
                     /translation="PGEKKNSDELARKIAALADVYVNDAFGTAHRAEATTHGAAKFAP
                     IACAGPLLAAEIEALGKALGNPKRPVAAIVAGSKVSTKLTLLNNLAQKVDRLIVGGGI
                     ANTFLLAEGFPIGKSLAEPDLVPECKKVLATLAAKGATLPLPIDVVVAKEFSATAPAT
                     VRRADDVRADEMILDIGPTSALALSHLLRDAGTIIWNGPIGVFEFDAFADGTHQMALA
                     IAEATANGAFSIAGGGDTLAAIARYGIGDRISYISTGGGAFLEFLEGKTLPAIEILQA
                     RAAA"
     gene            872..1303
                     /locus_tag="VLN25_08870"
     CDS             872..1303
                     /locus_tag="VLN25_08870"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSQ10713.1"
                     /translation="MSRFAVAGLDHVHVYVRDRAAAARWYGSVLGLRRDRRFAAWARE
                     VGGPLTLTAADSVTHVALFEDDRRAGHGTTVAMRTDGEGFVAFSRRAARLPLFDKRRQ
                     PAPPRLQDHVQSLSLYFNDLDGNPVELTTYDAAIARPRLRG"
     gene            1357..2793
                     /gene="pyk"
                     /locus_tag="VLN25_08875"
     CDS             1357..2793
                     /gene="pyk"
                     /locus_tag="VLN25_08875"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004188973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HSQ10714.1"
                     /translation="MARSTKIVATLGPASTDALVLERMLAAGVDVVRFNFSHGSASDH
                     LQRAERVREAAARIGRDIAIMADLQGPKVRVGKFEQGHVTLHAGAAFVLDASAERGNQ
                     DRVGLDYKDLPKDVRTGDTLLLNDGLIVLDVDRVEGSEIHTTVRIGGVLSDNKGINKQ
                     GGGLSAPALTGKDVRDIETAMQCGADYVAVSFVKNRTDVEMARQLATIAGEKHGVKPQ
                     IIAKIERAEAIPLLKEILEACDGIMVARGDLAVEVGNAAVPALQKRMIKMARAANKLA
                     ITATQMMESMIVNAVPTRAEVSDVANAVLDGTDAVMLSAETAAGKYPVEVVEAMASIV
                     EAADRSETVGLDMQFINQTFTRVDQSIAYGALFTAFHLRCAAICALTESGSTTLWMSR
                     HDVDIPIYALTPSASTARRLALYSNVRPLHFAAGADRDAALAGVERLLLDKGVVKRGD
                     LVVLTVGEPMGQAGGTNTLKIVRVGEHA"
     gene            2910..3974
                     /gene="fba"
                     /locus_tag="VLN25_08880"
     CDS             2910..3974
                     /gene="fba"
                     /locus_tag="VLN25_08880"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485500.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HSQ10715.1"
                     /translation="MALVSMRQLLDHAAENGYGVPAFNVNNLEQVQAIMEAANEINAP
                     VIMQASAGARKYAGELFLKHLIQAAVESYPQLPVAMHQDHGQSPAVCEGAMQLGFSSV
                     MMDGSLREDGKTIADYDYNWQVTRKVVDMAHAIGVSVEGELGCLGSLETMKGDKEDGH
                     GTESTMTRDQLLTDPDQAADFVKKTQLDALAIAIGTSHGAYKFTRKPTGDILAIERIK
                     QIHKRIPNTHLVMHGSSSVPQNLLEEIRKFGGQMKETYGVPVEEIQEAIKHGVRKINI
                     DTDIRLAMTAAIRRFLHENPGHFDPRDYLKPARAAAKAICKERYLQFGCEGQGSKIQP
                     VPLQMVAHKYQTGELSQHVH"
BASE COUNT          806 a         1342 c         1403 g          675 t
ORIGIN      
        1 ccgggcgaga agaagaacag cgacgaactc gcccgcaaga ttgccgcgct ggccgacgtc
       61 tacgtcaacg atgccttcgg caccgcgcat cgcgccgagg cgaccacgca tggcgctgcc
      121 aagttcgcgc cgatcgcctg cgccggccca ttgctcgcgg cggagatcga ggccctgggc
      181 aaggcgctcg gcaacccgaa gcggccggtg gccgcgatcg tggcgggctc gaaggtctcg
      241 accaagctca cgctgctaaa caacctggcg cagaaggtcg atcggctgat cgtcggcggt
      301 gggatcgcca acactttcct gctggccgaa ggctttccga tcgggaagtc gctggccgag
      361 cccgacctcg tgccggagtg caagaaggtt ctggcgacat tggccgccaa gggcgccacg
      421 ctgccgctgc cgatcgatgt cgtcgtggcg aaggaattct ccgcgacggc gccggcgacc
      481 gtccgacgcg cggacgacgt gcgcgccgac gaaatgatcc tcgacatcgg gcccacgagc
      541 gcgctggcgc tgtcgcatct gctgcgcgat gcgggcacga tcatctggaa cggacccatc
      601 ggcgtgttcg agttcgatgc gtttgccgat ggcacgcatc agatggcact ggcgattgcc
      661 gaggcgaccg ccaatggtgc cttttccatc gccggcggag gggacacgct ggcggcgatc
      721 gcccgttacg gcattggcga ccgcatcagc tacatctcga ccggcggcgg tgcattcctg
      781 gagttcctcg agggcaagac gctgcctgcg atcgagatcc tgcaggcgcg tgccgcggca
      841 tgatggttat gccgtttcca ggagagcttc gttgagccga tttgccgtcg cgggactgga
      901 ccacgtgcat gtctacgtgc gtgaccgcgc ggctgccgcg cgctggtacg gctccgtact
      961 gggtctcagg cgcgaccgcc gctttgcggc ctgggcccgc gaggtcggcg gtccgctcac
     1021 gctgacggcc gccgacagcg tcacgcacgt ggcactgttc gaggacgacc ggcgcgccgg
     1081 tcacggcacc acggtggcca tgcgcaccga tggcgaggga ttcgttgcct tctcgcggcg
     1141 ggccgcgcgg ctgccgctgt tcgacaagcg ccgccagccg gcaccgccgc ggttgcagga
     1201 ccatgtgcag tcgctgtcgc tgtacttcaa cgacctcgac ggcaacccgg tcgaactcac
     1261 cacctacgat gcggcgatcg cgcgtccgcg cctgcgcgga taggatgcgc accggcgctg
     1321 tccgctggca tcattcactg acgaggagcc gctgaaatgg cgcgcagcac gaagattgtg
     1381 gcgaccctcg ggccggcctc gaccgatgca ctggtcctcg aacggatgct ggcggccggc
     1441 gtcgatgtcg tgcgcttcaa cttctcgcac gggagcgcca gcgatcatct gcagcgcgcc
     1501 gagcgcgtgc gcgaggccgc cgcccggatc gggcgcgata tagcaatcat ggccgacttg
     1561 cagggtccga aggtgcgggt cggcaaattc gagcaaggac acgtcacgtt gcacgccggc
     1621 gccgcgttcg tccttgacgc gtcggccgaa cgcggtaacc aggatcgggt cggtctcgac
     1681 tacaaggacc tgccgaagga tgttcgcacc ggtgacacac tgttgctcaa cgacggattg
     1741 atcgtccttg atgtcgatcg ggtcgaaggc agcgagatcc atacgaccgt gcgcatcggc
     1801 ggtgtcctgt cggacaacaa gggcatcaac aagcagggcg gcggtctttc ggcgccggcg
     1861 ttaaccggca aggatgtccg agacatcgag acggcgatgc agtgcggcgc cgactatgtc
     1921 gcagtctcgt tcgtcaagaa ccgcaccgat gtcgagatgg cgcgccagct cgcgacgatc
     1981 gccggcgaga agcacggcgt gaagccgcag atcatcgcga aaatcgagcg cgccgaggcg
     2041 atcccgctgc tcaaggagat tctggaggcc tgcgacggaa tcatggtcgc gcgcggtgac
     2101 ctggcggtcg aggttggaaa cgccgcggtg ccggcactgc agaaacgcat gatcaagatg
     2161 gctcgcgcgg ccaacaagct tgcgatcacg gccacgcaga tgatggagtc gatgatcgtc
     2221 aatgcggtgc cgacgcgcgc ggaagtcagc gatgtggcca acgcggtgct cgacggcacc
     2281 gatgcggtga tgctatccgc ggagaccgcg gcgggcaagt acccggtcga ggtcgtcgag
     2341 gcgatggcga gcatcgtcga ggccgccgac cgctcagaaa cggttggact ggacatgcag
     2401 ttcatcaacc agactttcac acgcgtcgac cagtcgatcg cttatggcgc gctcttcacc
     2461 gcgttccatc tgcgctgcgc ggcgatatgc gcgctgacgg aatcgggttc gaccacgctg
     2521 tggatgagcc ggcacgatgt cgacataccg atctacgcgc tgacaccgag tgcctcgacc
     2581 gcgcgtcgcc tggcgctgta cagcaacgtg cggccactgc atttcgcggc cggcgccgat
     2641 cgcgatgccg ccctcgccgg tgtggagcgt cttttgctcg acaagggcgt ggtcaagcgc
     2701 ggcgacctcg tagtgctgac ggtcggcgag ccgatgggac aggccggcgg gaccaacaca
     2761 ctcaagatcg ttcgcgtcgg cgagcacgcc tgacgcttgc gggccgattg tccactgcga
     2821 ggtcagggca agagtcattc gcggcgacgc gacgactaaa atgtccgggc gaacacgaag
     2881 ttccggtcct actcattcag gagaatcaga tggcgttggt ctcgatgcga cagttactgg
     2941 accatgcggc ggaaaacggg tacggcgtcc ccgcgttcaa cgtcaacaac ctcgaacagg
     3001 tgcaggccat catggaggct gccaatgaga tcaatgcacc cgtcatcatg caggcctcgg
     3061 caggcgcgcg gaagtatgca ggcgagctct ttctcaagca cctgatccag gcggctgtcg
     3121 agagctaccc gcaactgccc gtcgcgatgc accaggatca tggccagtcc ccggccgtgt
     3181 gcgagggggc gatgcagctt ggcttctcat cggtgatgat ggacggttcc ctgcgcgaag
     3241 acgggaagac catcgcggac tacgactaca actggcaggt cacgcggaag gtcgtcgaca
     3301 tggcgcatgc gatcggcgtc agtgtcgaag gtgaactcgg ttgccttggg tcgctggaaa
     3361 cgatgaaagg cgataaggag gacggccacg gcaccgaatc gacgatgacg cgcgaccagc
     3421 tgctgaccga ccccgaccag gccgccgact tcgtgaaaaa gacgcagctc gacgcgctgg
     3481 ccatagccat cggcaccagc cacggcgcct acaagttcac acgcaagccg accggcgaca
     3541 tcctcgcgat cgagcgcatc aagcagatcc acaagcgcat ccccaacacg cacctggtga
     3601 tgcacggctc gtcgagcgtg ccgcagaatc tgctcgaaga aatccgcaag ttcggcggtc
     3661 agatgaagga aacctacggc gtgccggtcg aggagatcca ggaagcgatc aagcacggcg
     3721 tacgcaagat caacatcgac accgacattc gcctcgcaat gaccgcggcg atccgcaggt
     3781 tcctgcacga aaatcccggc cacttcgacc cacgcgatta cctcaaaccc gcgcgcgcgg
     3841 cggccaaggc gatctgcaag gagcgctacc tgcagttcgg ctgcgagggc cagggcagca
     3901 agatccagcc ggttccgctg cagatggtgg cgcacaagta ccagacgggc gaactgagtc
     3961 agcacgtgca ctgatccgcg gcttgcagcg aatgaaatcg gcgcggggtg cgccgttttc
     4021 attcatgtgc cccgcccggc gaagcaagtc gaagtgcggc ccgcttgcgc aggatcaatt
     4081 cgtgtcagct cctaggtact agttcgggtc gacgcaagcg agtgcgcggc ggatcatcca
     4141 tggaggcgca tcagacgccc gctgattcca aaggagaccg acatgaccac gaacccacgt
     4201 cgttgcccga tctgcaagtc ggaaac
//
LOCUS       DATIRT010000396        13540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1837550, whole genome shotgun sequence.
ACCESSION   DATIRT010000396 DATIRT010000000
VERSION     DATIRT010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13540
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1837550"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..662)
                     /locus_tag="VLN25_08885"
     CDS             complement(180..662)
                     /locus_tag="VLN25_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10716.1"
                     /translation="MPLDHLHLPAEARFWPAAARAVAHFAIRHGAPERQLQSVTWLVP
                     GGQHAELARAALRSELGGRAFMPPRIVPLTAWLGHPMATGTAAQVELFIALRANRWVR
                     EAFGAQSATLWALARDVAHLCDELTLASVSGAEDFDGRLQDSLRRHFHRRAAHALQAQ
                     "
     gene            865..1188
                     /gene="trxA"
                     /locus_tag="VLN25_08890"
     CDS             865..1188
                     /gene="trxA"
                     /locus_tag="VLN25_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HSQ10717.1"
                     /translation="MSEIKYVSDSSFEQDVLKSDTPVLVDYWAEWCGPCRMIAPILDE
                     VSKEYGGKVSVAKLNVDENQSTPAKYGIRGIPTLMLFKNGAVVGTKVGALSKSQLTAF
                     IDSNI"
     gene            1279..2541
                     /gene="rho"
                     /locus_tag="VLN25_08895"
     CDS             1279..2541
                     /gene="rho"
                     /locus_tag="VLN25_08895"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HSQ10718.1"
                     /translation="MHLSELKALHVSQLLEMALGLEIDNANRLRKQELMFAILKKRAK
                     SGEQIFGDGTLEVLPDGFGFLRSPETSYLASTDDIYISPSQIRRFNLHTGDSIEGEVR
                     TPKDGERYFALVKVDKVNGVAPEATKHRILFENLTPLFPNQPLILERDMKGEENITGR
                     LIDIIAPIGKGQRGLLVASPKSGKTVLMQHIAHAIAANHPDVVLIVLLIDERPEEVTE
                     MQRSVRGEVVASTFDEPATRHVQVAEMVIEKAKRLAESKKDVVILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDDVIYEE
                     FKGTGNMEIHMERRLAEKRVYPALNVNRSGTRKEEQLLKPDILQKVWILRKLLYGMDE
                     IEAMEFLLDKMKATKNNAEFFDMMRRGG"
     gene            2901..3200
                     /locus_tag="VLN25_08900"
     CDS             2901..3200
                     /locus_tag="VLN25_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HSQ10719.1"
                     /translation="MKSGIHPEYREVVFQDMQNDFKFITRSTLQTRDSIEFEGKTYPL
                     FKLDTSSESHPFYTGAQTRIVETGRVEKFRQKFAAKSEAVNAAAAQANAAKARRR"
     gene            3290..5026
                     /locus_tag="VLN25_08905"
     CDS             3290..5026
                     /locus_tag="VLN25_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10720.1"
                     /translation="MSFEHRPSPSRVTEAAAATFPRWLLFSLLAVYIVPGLFGRAPWS
                     PDDASAFGVAWSMATGSSLEWWLPSVAGEPLPEEGPLPFWVGALFAQWFGPWLGDVTA
                     ARLVTLLWFTIGTWALWYATYRLARRDEAQPVALAFGGEASPRNYGRMLADIAVLLLT
                     ATLGVLVRIHETGAEPALLALVCVLLFALAYSLDDPWKGAFVAGATLGAIALTRGWLP
                     AGVLLLAAIVFTGVYGDRRIERATLITLIAFLVFSLWPTGARLAHPQEAESYLAEWWR
                     WNRASVGMPQAEGLLWLARNVGWYAWPLWPFALWTIYSWRHFLRRPHMALPLIIVGAG
                     LVAVLLSASPSDREFLLAVPALVVLAAFGVSSLKRTAEEAIDWFSLALFSLALCSLWL
                     YFGAWNTGVPPKMSASVVRLAPGFEPDVHVATALLAIAATVLWIAIVVWRIRVRPPMV
                     WTGPFIAASGLSLLWLAAVALFAPAIDYARSYASIAPVLAQQIDRVGGDSCVQTVGLP
                     AGTRAMLAYHGGIRFERPADAGLCRVALQRDSQRTAVDDAPPAGRWKLAYDVTRRARF
                     DEAFRVWVRQPD"
     gene            5060..6421
                     /locus_tag="VLN25_08910"
     CDS             5060..6421
                     /locus_tag="VLN25_08910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HSQ10721.1"
                     /translation="MTHRPAPDASTSAVLKLAWPLFFANLAVVGNGTIDAVMAGRLSA
                     EDMAGVAVASSVYVTVYIGFMAVLQALSPIAGHHYGAQRWRAIGDDLQQALWLSALLL
                     IVALPCVLATGFWNGIASVDTRVAGITTSYLHAVAFGLPAGLATRVFVALNAAVSRPM
                     VTMVINLIVLGLKAPLNAVFMYGWGPFDAMGGAGAGVATALLAWLALGMSVAIWRLDP
                     FYDRFRSGSVHGPRWARLRELLRLGMPIGLSTLFEVTSFTFMAVLIARLGATSVAGHQ
                     IVANLVAILFMVPLSLGIATSVLIAQSLGSGAPRIARMAALRGFRLAFSVALVAALLL
                     WLLRGPVVHLYTGDPNVARMALSLLGLAALFHVFDAVQGMGSFVLRGYRQTFWPMVIY
                     GVALWGLGLGGGAWLAYNPSFLGPPRGAAGFWEAATLAIGITAAALAWLAAFEARRHI
                     TDH"
     gene            complement(6439..7179)
                     /locus_tag="VLN25_08915"
     CDS             complement(6439..7179)
                     /locus_tag="VLN25_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HSQ10722.1"
                     /translation="MLTGAPGHHVSLGRWELPIELLVVANLIALAFETLPELSPFWRQ
                     ALWSFEVFCAAVYTVEYGVRLYLARPRWKYATSFYGIIDLLAIAPFYLMVAFNLQAVR
                     AFRLLRLLRLFKLVRYTNAVHRFHRAFVIARDDLVLFGVIALIVLYLSAIGIYHFEHD
                     AQPERFSSVFDSLWWAVATLTTVGYGDIFPVTAGGRMFTFIVLVCGLGVIAVPSGLMA
                     SALSRVRAEEQAHQQAATERAQERNWSD"
     gene            complement(7296..8180)
                     /gene="htpX"
                     /locus_tag="VLN25_08920"
     CDS             complement(7296..8180)
                     /gene="htpX"
                     /locus_tag="VLN25_08920"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HSQ10723.1"
                     /translation="MKRIALFVVTNLAIMIVLMLFLTVLSSVFGINFNLGGRTLDVGA
                     LAIFSLVVGFTGAFISLLMSKPMAKWSTKAQVIRQPANAMESWLVDTVRRHADKAGIG
                     MPEVAVYEGEPNAFATGAFKNSALVAVSTGLLQSMSKEEVEAVLAHEISHVSNGDMVT
                     MTLLQGVMNTFVVFLSRVIGFFVDRVLLRNEGDRPGIGYFVTTIVLDIALGFLAAIVV
                     AWFSRRREFRADAGAAALMGSNRPMINALARLGGMEPGQLPQAVQTLGISAPGGVMAL
                     FSSHPPIEQRIAALQATR"
     gene            8434..9066
                     /locus_tag="VLN25_08925"
     CDS             8434..9066
                     /locus_tag="VLN25_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HSQ10724.1"
                     /translation="MSLPSTDAANVRPIPLVPRLIFASRWLQLPLYIGLIIAQVVYVF
                     HFWVELVHLVEAAFGSEPALQQLITSIGYKSDGLPSGLNETIIMLVVLALIDVVMISN
                     LLIMVIVGGYETFVSRMYLEKHPDQPEWLSHVNASVLKVKLATAIIGISSIHLLKTFI
                     NAANYSDKVLIAQTAIHITFLFSAMAIAATDRIMFPADKMKPKESRTHDH"
     gene            9076..10599
                     /locus_tag="VLN25_08930"
     CDS             9076..10599
                     /locus_tag="VLN25_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HSQ10725.1"
                     /translation="MTTIRQADLIESVAAALQFISYYHPADFISHLARAYEAEQSPAA
                     KDAIAQILTNSRMCAEGHRPICQDTGIVNVFLKVGMDVRWDGFTGSLEDAINEGVRRG
                     YLNSDNVLRASVVSPPEFGRKNTKDNTPAVINVQIVPGSTVDVMVAAKGGGSENKSKF
                     VMLNPSDSIVDWVMKTVPTMGAGWCPPGMLGIGIGGTAEKAMTMAKEALMDDIDMSDL
                     LKRGPQTDVEKLRVELYEKVNALGIGAQGLGGLSTVLDVKIRMYPTHAAGKPVAMIPN
                     CAATRHAHFVLDGSGPAYLEPPSLDTWPDVNWQPDAKKSTRVNLDTLTKAEVAQWKPG
                     QTLLLNGKMLTGRDAAHKRIQDMLAKGEKLPVDFTNRVIYYVGPVDPVRDEVMGPAGP
                     TTATRMDKFTEMMLAQTGLIAMIGKAERGPVAIEAIRKHKSAYLMAVGGAAYLVAKAI
                     KGSKVVGFSDLGMEAIYEFEVKDMPVTVAVSADGTSVHQTGPKEWQAKIGKIPVAVA"
     gene            10616..11704
                     /locus_tag="VLN25_08935"
     CDS             10616..11704
                     /locus_tag="VLN25_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HSQ10726.1"
                     /translation="MVVHAPVPRAWCDEAVRRIEADYTRSADTHLLRVDIPGVPDCPL
                     YLKDESVHPTGSLKHRLARSLFLYGLCNGLIGPGTTVVEASSGSTAVSEAYFARLIGV
                     PFVAVMPRATSPEKIALIEFHGGRCHLVEEPAAVYAEAAALAQRSGGHYMDQFTYAER
                     ATDWRGNNNIAESIFMQMAHEPFPVPTWIVVGAGTGGTGSTLGRYARYCRHATRICLA
                     DPENSVFHNYCASGDASLTMAVASRIEGIGRPRVEPSFNRHVIDRSIRVTDADSFAAA
                     RFLERVLGRRVGPSTGTALIACAELLSEMQSTGAKGAVVTLLCDGGERYLGTCYDDDW
                     LRRQALDIAPALAALAHFWSNGRWTALR"
     gene            complement(11721..12626)
                     /gene="cysM"
                     /locus_tag="VLN25_08940"
     CDS             complement(11721..12626)
                     /gene="cysM"
                     /locus_tag="VLN25_08940"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HSQ10727.1"
                     /translation="MPHLTIDQTIGNTPLVRLQRLPGPENTRRGNVILGKLEGNNPGG
                     SVKDRPALSMITLAEERGDIRPGDRLVEATSGNTGIALAMIAAMKGYRMTLIMPDNLS
                     SERRASMAAYGAELILTPASKGGMEYARDLAMQMQAEGKGKVLDQFANQDNPRSHYET
                     TGPEIWRDTDGRITHFVSAMGTTGTIMGAGRFLRERNAAVQIIGAQPAEGSQIPGIRK
                     WPDAYLPRIYDPAGVDRLEFVTQSDAEGMARRLAVEEGISCGPSAAGACLIALRIAAE
                     IEDATIVFVVCDRGDRYLSTGLFGV"
     gene            complement(12715..>13540)
                     /locus_tag="VLN25_08945"
     CDS             complement(12715..>13540)
                     /locus_tag="VLN25_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HSQ10728.1"
                     /translation="QDYLKGGLLDRAEDAFTRLLEDRDHRFDALRALLRIFQMEREWM
                     KAIDCARLLEREAGENHQVAIAHFHCELAEAAAAAGRIDDAQRAVDDAMTTDRKSVRA
                     LILAGDLAAHRGDAEGALRLWQRVEGASREHVPLIAGKVSETLDQLGRRTEALNWLRR
                     ELLDVPSIDLLDAAYIRARDWDGAEAAEALVADELKRHPSLLGFERLAEARLALNPGD
                     EELVSMSTLLKGHARKLARYRCSKCGFRAREFHWNCPGCTSWDSYPPRRIEEIDAA"
BASE COUNT         2434 a         4447 c         4286 g         2373 t
ORIGIN      
        1 cgatcgatga atgcgcgctc ccacgtcgcc agtcgcgccc gcccggcggt ggacagcccc
       61 gtgccgagat acaccagcgg cagctgggtt cgcgaggcgc gcgcagcgag ttcccgcagg
      121 aaccgcgcgg caccgtcgtc ggcctggcga cgggcgtgcc aaagctggat gaccagttgc
      181 tactgcgcct gcagcgcatg cgccgcacgg cgatggaagt gccgccgcag gctgtcctgc
      241 agcctgccgt cgaaatcctc ggcaccgctg actgaagcga gcgtcagttc atcgcacaag
      301 tgagccacat cacgcgcgag tgcccacaag gtcgcagatt gcgctccgaa tgcctcgcgg
      361 acccagcggt ttgcgcgcaa tgcgatgaac agttccacct gggcggccgt gccggtggcc
      421 atcggatggc cgagccatgc cgtgaggggc acgatgcgcg gcggcatgaa cgcgcggcca
      481 cccaactcgc tgcgcagggc ggcgcgcgcg agttccgcat gctgcccccc gggaaccagc
      541 catgtcaccg attgaagctg ccgttcgggg gcaccatgac gtatcgcaaa gtgggccacg
      601 gcgcgggcgg cggccggcca gaaccgggcc tcggccggaa ggtgaaggtg atcgagcggc
      661 atgaggcgat tgtaggaagt ccgcgatcgg tgaaatttgg tggctaaacc ggccgcgctt
      721 cgtacccacg cagaacgggt gcgcagtgtt accattcgct tcgaacttgc cagccaccct
      781 gataccgggg ttgtgccccg gcccgagcat ccccactttc catccttgtg aatgcgcgct
      841 gtccccggcg ccagaaggac tcaaatgagt gaaatcaagt acgtcagcga ctccagcttc
      901 gagcaggatg tcctcaagtc cgatacaccg gtcctggtgg actactgggc cgagtggtgc
      961 ggaccctgcc gcatgatcgc ccctatcctg gacgaggttt ccaaggaata cggcggcaag
     1021 gtctcggtgg ccaagctcaa cgtcgacgaa aaccagtcga ccccggcgaa gtacggcatc
     1081 cgtggcatcc cgacgttgat gctgtttaaa aatggcgcgg tggtcggcac caaggtcggc
     1141 gcactgtcca agtcccaact cacggcattc atcgacagca acatctgatc gtctcccggg
     1201 ccggcgcttg ccggcccgcc cgccccgcgg gcgcccggaa cagtcaaagg gcgtcccact
     1261 actcctccgg ttgtccccat gcacctctca gaactcaaag ccctgcacgt ttcccagttg
     1321 ctcgaaatgg ccctcggcct cgaaatcgac aacgccaacc ggttgcgcaa gcaggagttg
     1381 atgtttgcga tcctgaagaa gcgcgccaag agcggcgagc agatcttcgg cgacggaacg
     1441 ctggaagtcc tccccgacgg ctttggcttc ctgcgctcgc ccgagacgtc gtacctcgcc
     1501 agcaccgacg acatctacat ctcgccgtcg cagatccggc gcttcaacct gcacacgggc
     1561 gactcgatcg aaggcgaggt gcgcacgccg aaggacggcg agcgctattt cgctctggtc
     1621 aaggtcgaca aggtcaatgg cgtcgcaccg gaggccacca agcaccgcat cctgttcgag
     1681 aacctgacgc cgctgttccc caaccagccg ctgatcctcg aacgcgacat gaagggcgag
     1741 gagaacatca ccggccgcct gatcgacatc attgcaccga tcggcaaggg ccagcgcggc
     1801 ctgctggtcg cgagccccaa gagcggcaag acggtgttga tgcagcacat cgcgcatgcg
     1861 atcgccgcca accatccgga cgtcgtgctg atcgtgctgc tgatcgacga gcgaccggaa
     1921 gaagtgacgg aaatgcagcg ctcggtccgc ggtgaagtgg tcgcttcgac gtttgacgaa
     1981 ccggccacac ggcacgtgca ggtcgccgaa atggtcatcg agaaggccaa gcgtctggcg
     2041 gagtcgaaga aggatgtggt catcctgctc gactcgatca cgcgcctcgc gcgcgcctac
     2101 aacacggtgg tgccggcctc aggcaaggtg ctgaccggcg gtgtcgacgc caacgcgctg
     2161 cagcgaccca agcgcttctt cggagccgca cgcaacatcg aggaaggcgg atcgctcacg
     2221 atcatcgcga ccgctctgat cgacaccggc tcgcgcatgg acgacgtcat ctacgaggag
     2281 ttcaagggca cgggcaacat ggaaatccac atggagcgcc gcctagccga aaaacgcgtg
     2341 tacccggcgc tcaacgtcaa ccgctccggc acacgcaagg aagaacaact gctcaagccc
     2401 gacatcctgc aaaaggtctg gatcctgcgg aagctgctct acggcatgga cgaaatcgaa
     2461 gcgatggaat tcctgctcga caagatgaag gcgaccaaga acaacgccga gttcttcgac
     2521 atgatgcggc gaggtgggtg agacgggggt tgcgtttgat gcctgcgcat caatcgtgcc
     2581 ggtcgaaccg gccgcgcctg tgagcgcggc cgtgggccga acgcgaagta ctcggcgcga
     2641 agcgccatca acgcgatcgg atgcctcgcg caaaattgca cccctactct tgcgcgccat
     2701 gaaatgaaaa agatagctgg cgaagtcagt cgatcttttt taaaacaacg gcttgcaact
     2761 gcttgcggct tgcccgagac cctgatttcc cggataatgc gcggttcccg aaaaacccag
     2821 ggaccgtggt cgaggcagac cggggcagat gaattcagtc acccctcgac cgggcggccc
     2881 acgacaaggc aagaaggctt atgaaatcag gcatccatcc cgaataccgc gaagtcgttt
     2941 tccaggacat gcagaacgac ttcaagttca tcacgcgctc cacgctgcag acgcgcgatt
     3001 ccatcgaatt cgaaggcaag acctatccgc tgttcaagct cgacacgtcc tcagagtcgc
     3061 accccttcta caccggggcg cagacccgca tcgtcgagac cggtcgcgtc gagaagttcc
     3121 gccagaagtt cgcggccaaa agcgaagcgg tcaatgcggc cgccgcccag gccaacgctg
     3181 ccaaggctcg gcgcaggtaa ggagccctcg gggcgaatga agacagggca gcctcggctg
     3241 ccctttttgt tgccccggcc actcttctcc cagaccacat ctagcgcgca tgagcttcga
     3301 acaccgaccg tcgccttcgc gcgtgaccga ggccgccgca gccacgtttc cacgctggct
     3361 gctgttttcg ctgctggccg tctacatcgt gcctgggctg ttcggccgcg caccctggag
     3421 ccccgatgac gccagcgcct tcggcgtggc gtggagcatg gcgacgggct cgagtcttga
     3481 atggtggctg ccatcagtgg ccggcgaacc cctgccggaa gaaggcccgc tgcctttctg
     3541 ggtcggcgca ttgttcgcac agtggttcgg accctggctt ggggacgtca ccgccgcacg
     3601 cctggtcaca ctgctctggt tcaccatcgg cacctgggca ttgtggtacg cgacctaccg
     3661 gctcgcccgc cgtgatgagg cgcagcccgt tgccctggcc ttcggcggtg aagcgtcgcc
     3721 acgcaactac ggacgcatgc tggcggacat tgctgtcctg ctgttgacgg cgacgttggg
     3781 cgtgctggtg cgcattcacg agacgggcgc cgaacctgcc ttgcttgcgc tggtgtgtgt
     3841 gctgctcttc gcgctcgcgt attcgctcga cgatccgtgg aagggagcgt tcgtggccgg
     3901 cgccacgctc ggcgcaatcg cgctgacgcg cggttggctg ccggccggcg tgctgctgtt
     3961 ggccgccatc gtctttacgg gcgtctacgg cgatcgtcgc atcgagcgcg cgacgctgat
     4021 cacgctgatc gcatttctgg ttttttccct ctggccgacc ggcgcgcggc ttgctcaccc
     4081 gcaggaggcc gagtcgtacc tggccgaatg gtggcgctgg aaccgcgcaa gcgtcggcat
     4141 gccgcaggct gaaggcctgc tgtggctcgc ccgcaatgtc ggctggtacg catggccctt
     4201 gtggccgttc gccctctgga cgatctactc gtggcgccac ttcctgcgcc gtccgcacat
     4261 ggcactgccg ctgatcatcg ttggcgcggg gctcgttgcg gtgttgctgt cagcttcgcc
     4321 aagcgaccgc gagttcctgc tcgcggtgcc cgcgctggtc gtgctcgccg ccttcggcgt
     4381 gtcgtcgctc aagcggacgg ccgaggaagc gatagactgg ttttcccttg cgctcttcag
     4441 cctggcgctg tgctcgctgt ggctctactt cggcgcgtgg aacaccggtg tgccgccgaa
     4501 aatgtcggca tcggtcgtgc gactcgcgcc ggggttcgag cccgacgtgc acgtggcaac
     4561 cgccctgctt gccatcgccg ccaccgtgct gtggatcgcg atcgtggtct ggcgcatacg
     4621 cgtccgaccg ccgatggtgt ggaccggtcc cttcattgcc gcgtccggtc tgtcgctgct
     4681 gtggctggcg gccgtcgcgc tgttcgctcc ggctatcgat tacgcccgct cctatgcctc
     4741 gatcgctccg gtgctggcgc agcagatcga ccgcgtcggc ggtgactcgt gcgtgcagac
     4801 cgtcggtttg ccggcaggaa cccgcgcgat gctggcgtat cacggcggaa tccgtttcga
     4861 gcggcccgcc gacgccggcc tatgccgcgt ggccctgcag cgggacagcc agcgaacggc
     4921 cgtcgacgac gcgccgccgg ccggccgctg gaagctcgcc tacgacgtca cccggcgcgc
     4981 gcgcttcgac gaagccttcc gtgtctgggt aagacagcct gactgacgac cggcacgtcg
     5041 gccgtcacgc ctcatgtcaa tgactcaccg gcccgcgccg gacgcctcca cgtcggcagt
     5101 gctgaagctc gcctggccgt tgttcttcgc caacctggca gtggtcggca acggcacgat
     5161 cgatgcggtc atggccgggc gcctgtcagc ggaggacatg gcgggagtgg ccgtagcctc
     5221 gagcgtctac gtcaccgtct acatcggctt catggcggtg ctgcaggccc tgtcgccaat
     5281 cgccggccac cactacggcg cgcaacgctg gcgtgccatc ggcgacgacc tgcagcaggc
     5341 cctttggcta tcggctttgc tgctgatcgt ggcgttgccg tgcgtgctcg ccaccggctt
     5401 ctggaacggc atcgccagcg tagacacacg cgttgcaggc atcacaacga gttacctgca
     5461 tgcggtggcc ttcggcctgc cagcgggcct ggctacacgt gtcttcgtcg cgctcaatgc
     5521 cgccgtctcc cgtccgatgg tcacgatggt catcaatctg atcgtactgg ggctgaaggc
     5581 tccgctgaat gcggtgttca tgtacggctg gggtcctttc gacgcgatgg gaggtgccgg
     5641 cgcgggtgtg gcgaccgcgc tgctcgcctg gctcgcgctg ggcatgagcg tggccatctg
     5701 gcgactggac cctttctacg accggtttcg cagcggatcg gtgcacggac cgcgctgggc
     5761 ccgactgcgt gaactgctgc gcctcggaat gccgatcggg ctgtcaacgt tattcgaagt
     5821 gaccagcttc accttcatgg ccgtgctcat cgcgcgcctc ggcgccactt cggtcgccgg
     5881 tcatcagatc gtggccaacc tcgtcgcgat tctgttcatg gtcccgctgt cactgggcat
     5941 cgccaccagt gtcctgatag cccagtcact gggcagcggc gcgccgcgca ttgcccgcat
     6001 ggcggccctg cgcggtttcc gactggcatt ttcggtggcg ctggtcgccg ccttgctgct
     6061 gtggctgctc cggggtccgg tcgttcacct gtacacgggc gatccgaacg tcgcccgcat
     6121 ggcgctgtcg ctgctcggcc tggccgccct gttccatgtg ttcgacgcgg tccaggggat
     6181 gggaagcttc gtgttgcgcg gctaccgcca gacgttctgg ccgatggtca tctacggtgt
     6241 cgcgctgtgg ggactcgggc tcggcggcgg cgcctggctt gcctacaacc cgagcttcct
     6301 tggaccaccg cgaggagcgg ccggcttctg ggaagccgcc acactcgcca tcggcatcac
     6361 agcggcggca ctggcttggc tggccgcatt cgaggcccgc cggcacatca ccgatcactg
     6421 aagccgggaa gccccgagct agtctgacca gttgcgttcc tgtgcccgct ccgtggcggc
     6481 ctgttgatgc gcttgttctt cggcccgcac gcgtgacagt gcagaggcca tcaggcccga
     6541 cggcacggcg attacgccga gtccgcacac cagcacgatg aaggtgaaca tccgccctcc
     6601 ggcagtgacc gggaagatgt ccccgtagcc cacggtcgtc agtgtcgcga cagcccacca
     6661 gaggctgtcg aacacggagg agaaccgctc cggctgcgca tcgtgctcga agtggtagat
     6721 gccgatcgcg gacaggtaca gcacgatcag cgcgatcacg ccgaacagaa cgaggtcatc
     6781 gcgtgcgatg acgaatgcgc ggtggaagcg gtggacggcg ttcgtatacc gcaccagctt
     6841 gaacagccgc aggagccgca gcaggcgaaa ggcccgcacg gcctgcaggt tgaaggccac
     6901 catcagataa aacggtgcga tcgccagcag gtcgatgatt ccgtaaaacg acgtggcgta
     6961 cttccagcgg ggccgtgcca gatagagccg cacgccgtac tcgacggtgt agaccgcagc
     7021 gcagaagact tcgaagctcc acagggcctg ccgccagaac ggcgacagct cggggagtgt
     7081 ctcgaatgcc agggcgatga ggttggcaac caccagcagc tcgatcggca gttcccatcg
     7141 accaagcgaa acgtggtgtc ccggtgcgcc agttagcaac gttgcctccg cagaaacggg
     7201 aattcttacc cgaaatcgga gcgacacccg atccgacaac caaaaaaaac ggccggggag
     7261 tccccggccg tccgacttca cagtgtgcca acgtgtcagc gcgtggcctg caacgccgcg
     7321 atccgctgct cgatcggcgg atggctggag aacagggcca tcacgcctcc cggggcggag
     7381 atgcccaggg tctgcaccgc ctgcggcagc tggccaggct ccatgccccc gagccgcgcc
     7441 agcgcattga tcatcggccg gttcgagccc attagcgcgg ccgcaccagc gtcggcacgg
     7501 aactcacggc gacgggagaa ccaggccacc acgatcgccg ccaggaatcc cagcgcgatg
     7561 tccagcacga tagtcgtgac gaagtagccg atgcccggcc ggtcgccttc gttacgaagc
     7621 aggacacggt cgacgaagaa tccgatcacg cgcgacagga acacgacgaa cgtgttcatc
     7681 actccctgca gcagcgtcat cgtcaccatg tcgccgttgg acacgtgcga gatctcgtgc
     7741 gcaagcaccg cctcgacttc ttccttgctc atcgactgca acaggccggt cgacaccgcc
     7801 accagcgccg agttcttgaa cgctcctgtc gcgaaggcgt tcggctcgcc ctcgtagacg
     7861 gcgacttcgg gcatgccgat gcccgccttg tcggcgtgcc ggcggaccgt gtcgaccagc
     7921 caggattcca tcgcattggc cggctggcgg atgacctgcg ccttggtgct ccacttggcc
     7981 atcggtttgg acatcagcag cgaaatgaag gcgccggtga atccgaccac cagcgagaag
     8041 atcgccagtg cgccgacgtc aagcgtgcga ccgccgaggt tgaagttgat cccgaatacc
     8101 gacgacagga cggtgaggaa cagcatcaac acgatcatga tcgcaaggtt cgtcacgacg
     8161 aacagagcga tacgtttcat agagtctcca gaaaagttga acagttaaag ccgggcgaga
     8221 gtctagccaa tcggcggatc agtacaccat acacgtttgt ccccagcgct acattgcgac
     8281 accctaaaat atcccttgag gatcaaggcg ttggaccgga atcccggcaa acgttccgca
     8341 tttggaggcg attcggcgca actcaagtgg gccatcggaa tgcatgggcc gcgctggcta
     8401 gaatacagcg ctttgattga cgagaatccg atcatgtccc tgccgagcac cgacgccgcc
     8461 aatgtccgac cgattccact ggtcccacgg ctgatctttg ccagccgctg gctgcagctg
     8521 ccactctaca tcgggctgat catcgcccag gtcgtctacg tctttcactt ctgggtcgaa
     8581 ctggtgcacc tggtcgaggc tgccttcggc agcgagcctg ccctgcagca actcatcacc
     8641 agcatcggct acaaatccga cgggctgccc tccgggctga acgaaaccat catcatgctg
     8701 gtcgtgctgg cgttgatcga cgtggtgatg atttccaacc tgctcatcat ggtcattgtc
     8761 ggcggttacg aaaccttcgt ctcgcgcatg tacctggaga agcaccccga ccagccggaa
     8821 tggctgtcgc atgtgaacgc aagtgtgctc aaagtcaaac tggccactgc catcatcggg
     8881 ataagctcga tccatctgct gaaaaccttc atcaacgcgg ccaactattc ggacaaggtg
     8941 ctgatcgcac agacggcgat ccacatcact ttcctgtttt cggcaatggc gatcgcggcc
     9001 accgaccgca tcatgttccc agccgacaag atgaaaccca aggaaagccg cacccacgac
     9061 cactagggtt ccaccatgac aacgatccgc caggccgacc ttatcgaaag cgttgcggcc
     9121 gcgctgcagt tcatcagcta ttaccacccg gcggacttca tcagccacct ggcgcgcgct
     9181 tacgaagccg agcagagtcc ggcggcgaag gacgcgatcg cccagatcct gaccaactcc
     9241 cgtatgtgcg cagaaggcca ccggccgatc tgccaggaca cgggcatcgt caacgtcttc
     9301 ctgaaggttg gcatggacgt gcgttgggat ggattcacgg gctcgctcga ggacgcgatc
     9361 aacgagggcg tgcgccgcgg ctatctgaat tcggacaatg tcctgcgggc atcggtcgtg
     9421 agtccgccgg aattcggccg caagaacacg aaggacaaca cacccgccgt catcaacgtg
     9481 cagatcgtgc cgggcagcac tgtcgacgtg atggtggccg ccaagggcgg gggctcggag
     9541 aacaagagca agttcgtcat gctcaatccg agcgactcga tcgtcgactg ggtgatgaaa
     9601 accgtgccga cgatgggcgc cggatggtgc ccaccgggaa tgctcggcat cggcatcggc
     9661 ggcaccgccg agaaggcgat gacgatggcc aaggaagcgc tgatggacga catcgacatg
     9721 tcggacctgc tgaagcgcgg gccacagacg gacgtcgaga agctgcgcgt cgaactctac
     9781 gagaaggtca acgcactggg catcggcgcc cagggcctgg gcgggctgtc caccgtgctc
     9841 gacgtcaaga tcaggatgta tccgacacac gccgccggca agccggtggc gatgattccc
     9901 aattgcgcgg caacccggca tgcgcacttc gtgctcgacg gctcgggccc cgcctacctc
     9961 gagccgccgt cgctcgatac ctggccggac gtgaactggc agccggatgc gaaaaagagc
    10021 acgcgcgtga atctcgacac gctgaccaag gccgaggtgg cccagtggaa acccggccag
    10081 acactgctgc tcaacggaaa gatgctgacg gggcgcgacg ccgcgcacaa gcgcatccag
    10141 gacatgctgg ccaagggaga gaaactgccc gtcgatttca ccaatcgcgt catttattac
    10201 gtcggccccg tcgatccggt gcgtgatgag gtcatggggc ccgccggtcc gacgaccgcc
    10261 acccgcatgg acaagttcac cgagatgatg ctggcgcaaa cggggctgat cgcgatgatc
    10321 ggcaaggccg agcgcgggcc ggtggcgatc gaggctattc gcaagcacaa gagcgcctac
    10381 ctgatggccg tcggcggcgc tgcctacctc gtcgccaagg cgatcaaggg atcgaaagtc
    10441 gtcggcttca gcgacctcgg catggaagcg atctatgagt tcgaggtgaa ggacatgccg
    10501 gtcaccgtcg cggtcagcgc cgacggtacc tcggtgcacc agaccgggcc gaaggagtgg
    10561 caggcgaaga tcggcaagat cccggtcgcc gtggcctgag acgcgcggtg gtgccttggt
    10621 agtacatgct cctgtcccgc gagcctggtg tgacgaggct gtccgccgca tcgaagcgga
    10681 ttacacgcgg tcggcggaca cgcacctact gcgcgtggac attcccggag tgccggactg
    10741 cccgctgtac ctgaaggatg agtcggtcca cccgacgggc agcctgaagc atcggctggc
    10801 acgttcgctg ttcctctacg gactatgcaa tggcctcatc ggccccggca cgacagtcgt
    10861 cgaggcgtcg tcggggtcga ccgcggtgtc cgaggcttat ttcgcgcgcc tgatcggcgt
    10921 gcccttcgtc gccgtgatgc cgcgcgcgac ctcacctgaa aagatcgcgc tcatcgagtt
    10981 ccacggcgga cgttgccatc tcgttgagga accggcggcg gtctatgccg aggcggccgc
    11041 cctggcacag cggtcgggcg gccactacat ggaccagttc acctacgcgg agcgcgcaac
    11101 ggactggcgc ggcaacaaca acattgccga gtcgatcttc atgcagatgg cccatgaacc
    11161 attcccggtc ccgacatgga tcgtggtcgg tgcgggcacc ggcggcaccg gttccaccct
    11221 cggtcgctac gcgaggtatt gccggcatgc cacccgcatc tgcctggccg atcctgaaaa
    11281 ctccgtcttc cacaattact gcgcaagcgg cgatgcatcg ctgaccatgg ccgtcgcttc
    11341 gcgtatcgaa ggtatcggcc gtccacgcgt cgagccgagc ttcaaccgcc acgtcatcga
    11401 ccggtcgatt cgcgtcacgg atgcggactc cttcgctgcc gcgcgctttc tcgagcgcgt
    11461 gctcggacgc cgcgtgggac cgtcgacagg aacggcgctg atcgcctgcg cagaactgtt
    11521 atcggaaatg caaagcaccg gcgcgaaggg tgcggtggtc acgctgctgt gcgacggcgg
    11581 tgagcgctac cttggcacct gctatgacga tgactggctg cggcgtcagg cgcttgatat
    11641 cgcacctgca ctcgcggcgc tggcccactt ctggtcgaac gggcgctgga cagcgttgcg
    11701 gtgacgcggc actcgctcct tcagacgccg aacaggcccg tgctgaggta acgatcgccg
    11761 cggtcgcaga cgacgaagac gatcgtcgcg tcttcgattt cggcagcgat ccgcagcgcg
    11821 atcaggcacg caccagcagc cgacgggccg caactgatgc cctcctcaac tgccaagcga
    11881 cgtgccatgc cttcggcgtc cgactgggtt acgaactcga gccgatcgac cccggccggg
    11941 tcatagatcc gcggcaggta cgcgtcgggc cacttgcgga tgccgggaat ctgggagcct
    12001 tcggccggct gcgcaccgat gatctgcacg gcggcgttgc gttcccttag gaagcggccc
    12061 gcgcccatga tcgtgccggt cgtgcccatt gcgctgacaa agtgcgtaat gcggccatcc
    12121 gtgtcgcgcc agatctcggg cccggtggtc tcgtaatgcg agcgcggatt gtcctgattg
    12181 gcgaactggt cgagcacctt gcccttgccc tcggcctgca tctgcatcgc gagatcgcgc
    12241 gcgtactcca tgccgccctt gctcgcgggc gtcaggatca gctcggcacc gtaggctgcc
    12301 atgcttgcgc gccgctcgga gctgaggttg tccggcatga tcagcgtcat gcgataaccc
    12361 ttcatcgcgg cgatcatcgc cagcgcaatg ccagtgttgc cgctggtcgc ttcgacgagc
    12421 cggtcgcccg ggcgaatgtc accgcgctcc tcggcaagcg tgatcatcga gagagctgga
    12481 cggtccttga ccgagccccc cggattgttg ccttccagct tgccgaggat cacgttgccg
    12541 cggcgtgtgt tctcgggacc gggcagccgc tgaagccgca ccagtggggt gttgcctatg
    12601 gtctggtcga tcgtcaggtg aggcatggcc ggaacgtcaa ggtagagctg agaaggtcgt
    12661 gaccagaggc atcgcggtca tcgacgcgca ggacttttgg tttttcggcc cggatcaggc
    12721 ggcatcgatt tcctcgatgc ggcggggcgg gtagctgtcc caacttgtgc agcccggaca
    12781 gttccagtga aactcgcgtg cgcggaagcc gcacttgctg cagcggtatc gtgccagctt
    12841 gcgcgcatga cccttcaaca gcgtgctcat cgaaaccagt tcctcgtccc ccgggttgag
    12901 cgcgagccgc gcctcggcaa gacgttcgaa cccgagcagc gatgggtgcc gcttcaattc
    12961 gtcggcgacc agggcttcgg ccgcctcggc accgtcccag tcgcgggcgc gaatgtatgc
    13021 ggcatcgagc agatcgatcg agggtacatc gagcaactcg cgtcgcagcc agttcagcgc
    13081 ttctgtgcgc cgtccgagct ggtcaagtgt ctcactcacc ttgcccgcga tcagcggcac
    13141 gtgctcccgc gaagcgccct cgacccgctg ccacagccgc agggctcctt ctgcgtctcc
    13201 ccggtgggcc gcaagatcgc ccgcaaggat cagcgcgcgc actgacttgc ggtctgtggt
    13261 catcgcatca tccacggcgc gctgcgcgtc gtcgatccga cccgccgctg cggcggcctc
    13321 tgccagctca cagtgaaagt gcgcaatggc gacctgatga ttctcgccag cttcgcgctc
    13381 gagcaggcgc gcgcagtcta tcgccttcat ccattcgcgc tccatctgga agatccgcag
    13441 caacgcacgc agggcatcga agcgatggtc gcggtcctcc agcagccgcg tgaaggcgtc
    13501 ctcggcacgg tcgagcagcc cgcccttcag atagtcctgt
//
LOCUS       DATIRT010000397         1954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1149914, whole genome shotgun sequence.
ACCESSION   DATIRT010000397 DATIRT010000000
VERSION     DATIRT010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149914"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..147
                     /locus_tag="VLN25_08950"
     CDS             10..147
                     /locus_tag="VLN25_08950"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10729.1"
                     /translation="MAVYPVDGATGDELIKSADSAMYRAKTQKSGCMFFDRGEKKPAS
                     T"
     gene            415..1518
                     /locus_tag="VLN25_08955"
     CDS             415..1518
                     /locus_tag="VLN25_08955"
                     /inference="COORDINATES: protein motif:HMM:NF014324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HSQ10730.1"
                     /translation="MFCSDAGRLSLAILALAASAQTAAQSPHDLERCRAMSEAAARLA
                     CYDAAAAAATRTAPKAPESAPTPAAPAPAATAPAEDGRSSPGSQLVATGTLAGGQSLI
                     AQRWELEDRYKGGTFRFTFYRPNYILPAVWSDEVNDAPSTPTQPTFVPPAPLDNVEAE
                     FQISFKMKMWEDMFGGPSALWLGFTQRSFWQVYNEELSRPFRETNYEPELIWTLPTDY
                     EVFGLKGRLLAFGLVHQSNGRGDPLSRSWNRVYGMVALERGPFVMQFKPWYRLSEDGH
                     EDDNPDIADYIGDAEFLFFYKWRHEYTATLRYRTTFKSDPSWGSAQLDLRFPITTYLK
                     GYFQLFSGYGESLIDYNFRKTSVGLGVSIGTWY"
     gene            1518..>1954
                     /locus_tag="VLN25_08960"
     CDS             1518..>1954
                     /locus_tag="VLN25_08960"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HSQ10731.1"
                     /translation="MCYFPMKGGPYGLRLTTFLNPIGMPCWNPPYGSIAAYDLKTGEQ
                     IWNRPFGAVQQWGFYMPDSWGSVTIGGPVVTASGLVFIGGSMDSRVRALDLKTGEVLW
                     KHLVSAPAVSMPAMYEYEGRQYVMFAVGGNSILTPRVSDELIA"
BASE COUNT          355 a          612 c          629 g          358 t
ORIGIN      
        1 agcataggaa ttgccgtcta ccctgtcgat ggcgctaccg gcgatgaact gatcaagagc
       61 gccgacagtg ccatgtaccg cgccaagact cagaagagcg gctgcatgtt cttcgacagg
      121 ggcgagaaga aaccggcctc gacctgaacg tcgcgccctc gacattggtt aggactgacg
      181 ccgcatggcg gcgggggcgc gtgctaggcc ggagacgcga cccgactggc gcccaaactc
      241 agatgctcgc cggtcctgat cgattggttc gggtccgggg cgctgggtgg ctttcaatcg
      301 atacactcgg gtctgaccgg aacggcaatc gtgtgactcg tcaggcgggg atcgtcccga
      361 gggcggacaa ctctcccgcg accggcgcaa agtccactcg aagaaaggac catcatgttc
      421 tgcagtgatg ctggccggct gtcgctggcc atcctcgcgc ttgcggcgtc ggcgcaaacg
      481 gccgcacaga gcccgcatga cctcgaacgc tgccgcgcca tgtccgaagc agcggcacgt
      541 ctggcgtgct acgacgctgc tgccgcagcg gcaacgcgta cggcaccgaa ggcgcccgag
      601 agcgcaccga cgccggccgc tccggcgcct gccgcgaccg cgccggccga ggatggacgc
      661 agctcacccg gatcgcagct ggttgccacc ggaacgctcg caggcggcca gtcgctgatc
      721 gcgcagcgct gggaactgga agacaggtac aagggcggaa ccttccggtt caccttttac
      781 cggccgaact acatcctgcc cgccgtctgg tccgacgagg tgaacgacgc accgtcgaca
      841 ccgacacagc cgacctttgt tccgccggcg ccgctcgaca acgtcgaagc cgagttccag
      901 atcagcttca agatgaagat gtgggaggac atgttcggcg gcccgtcggc gctgtggctg
      961 ggcttcacgc agaggagctt ctggcaggtc tacaacgagg agctttcgcg tccgttccgt
     1021 gagaccaact acgagccgga actgatctgg acgctgccga ctgactacga ggtcttcggc
     1081 ctgaaggggc ggctgctggc gttcgggctg gtgcaccagt ccaacgggcg cggcgatccg
     1141 ctgtcgcgca gctggaaccg cgtctatggc atggtcgccc tcgagcgcgg accgttcgtc
     1201 atgcagttca agccatggta tcgcctgtcc gaggatggcc atgaggatga caacccggac
     1261 atcgccgatt acatcggcga tgcggaattt cttttcttct acaagtggcg tcatgaatac
     1321 acggcgacgc ttcgttaccg cacgactttc aagtccgatc cctcgtgggg ttcggcacaa
     1381 ctcgacttgc gctttccgat cacaacgtac ctgaagggat acttccagct gttctcgggt
     1441 tacggcgaga gccttatcga ctacaacttc cgcaagacat ccgtgggtct cggcgtgtcg
     1501 atcgggacct ggtactgatg tgctacttcc cgatgaaggg cggcccgtat ggcctgcgcc
     1561 tgacgacctt cctcaatccg atcggcatgc cgtgttggaa ccccccgtat ggttcgatcg
     1621 ctgcgtacga cctgaagacc ggcgagcaga tctggaaccg gccgttcggc gcggtccagc
     1681 agtggggctt ctacatgccg gactcgtggg gcagcgtgac catcggcgga ccggtcgtca
     1741 ccgcctcggg actggtgttc attgggggct cgatggattc tcgcgtgcgc gcactcgacc
     1801 tgaagaccgg cgaagtactg tggaagcatc tggtgagcgc cccggcggtg tcgatgcccg
     1861 cgatgtacga atacgagggc aggcaatacg tcatgttcgc ggtcggtggc aattcgatcc
     1921 tgacgccccg tgtctcggac gaactgatcg cgtt
//
LOCUS       DATIRT010000398         3511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_264091, whole genome shotgun sequence.
ACCESSION   DATIRT010000398 DATIRT010000000
VERSION     DATIRT010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264091"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VLN25_08965"
     CDS             <1..432
                     /locus_tag="VLN25_08965"
                     /inference="COORDINATES: protein motif:HMM:NF020771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HSQ10732.1"
                     /translation="PFEQSMRMFLTPFLMSSRGGEDELDADVMRMHLREMIEPSDVFR
                     EITAQTIVPAHDALAKVLARHARLAAPDADIHQLAFAIVAMAHDYCMSREFMKMLAPE
                     VLDRPRAMELIVERLVGYSRALLDYEVARRRGPSPMESPSR"
     gene            429..1598
                     /locus_tag="VLN25_08970"
     CDS             429..1598
                     /locus_tag="VLN25_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSQ10733.1"
                     /translation="MKPLLPVSRRGRVTIFVAFIALVLAGAAAVATGQAVKDARSDTA
                     AKKLAASTRPALTVSTVAPQKAEWPRTISANGNITAWQEAIIGAELSGLRLTDVLVNV
                     GDTVKRGQLLARIAADAVSADLAQARASVVEAEASLAEAKANAQRSKQLQAQGFISPQ
                     ATIQVVTAEQTTAARIAAARARVQAEDVRLAQTRIVAPDDGVISARTATVGSLTQPNQ
                     ELFRLIRGGRLEWRAEVTAAELARLQPGVAASLTTPSGERITGRVRAVAPSVDPATRN
                     GIVYVDIAAGTAARAGTFARGEFELDRSPALTLPQSAVLLRDGFAYVFRIGPDNRVAQ
                     IKVELGRRVGDRIEVRDGIDATTRVVETGAGFLADGDTVRVVESPALPVKSAAKQ"
     gene            1616..>3511
                     /locus_tag="VLN25_08975"
     CDS             1616..>3511
                     /locus_tag="VLN25_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSQ10734.1"
                     /translation="MNVSAWSIRNPIPAVLLFVMLTLIGVLGFRGMKIQQFPDIDLPT
                     VTVVAALPGAAPAQMETEVARKIENSIATLQGIKHIYTKVQDGTATITVEFRLEKPTQ
                     EALDDVRDAVSRVRSDLPGDLKDPVVSKVNLAGSPILTYTVASPRMDDEALSWFVDNT
                     VTKNMLSVRGVGAVARVGGVNREIRVELDPARLLALKATAADISRQLRQIQQEASGGR
                     ADIGGIEQSVRTIATVQSAEELAAMDIALADGRRIRLDQVATVSDTVGEQRSAALLNG
                     MPVVGFEITRSRGAGEVDVAEGVRAQLAQLKSEHPDIQITEAFNFVDPVEENFTGSMW
                     LLIEGALLAVLVVWLFLRDWRATLVSAAALPLSILPTFGAMYLMGFTLNVVTLLTLSL
                     VVGILVDDAIVEIENIVRHMRMGKSPYQASMEAADEIGLAVIATTFTLIAVFLPTAFM
                     SGVPGKFFVQFGWTAAIAVFFSLVVARMLTPMMAAYLLRPPRKDHAEPRWMTIYLRWA
                     QWCLKHRIATSLAAAAFFFGSFALVPLLPTGFLPPDDLSQTQVYLTLPPGSTFNQTLA
                     TAEQARAIVEKHPHVKLVYTAIGGGAAGSDPFAPRGAAEARKATLTINLTPRSDRRGI
                     SKQSVERE"
BASE COUNT          581 a         1196 c         1164 g          570 t
ORIGIN      
        1 ccgttcgagc agtcgatgag aatgttcctg acaccgttcc tgatgtcttc gcgtggcggc
       61 gaggacgaac tcgatgccga cgtaatgcgc atgcacctgc gcgagatgat cgagccgtcc
      121 gacgtctttc gcgagatcac cgcccagacc atcgttccgg cccatgacgc actggcgaag
      181 gtgctggcac gccacgcgcg cctggccgcg ccggatgcgg acattcacca gctagccttt
      241 gcgatcgttg cgatggcgca tgactactgc atgtcgcgcg aattcatgaa aatgctcgcc
      301 cccgaagtac tcgaccggcc ccgcgcaatg gagctgatcg tcgagcgcct ggtcggctat
      361 agccgtgcgc tgctcgacta cgaagttgcg cggcggcgcg gcccgtctcc gatggagtcg
      421 ccgtcccgat gaaaccgctg cttcccgttt cgcgtcgcgg cagggtgacg atcttcgtcg
      481 cattcatcgc gctcgtcctc gcgggcgccg cggccgtggc caccggacag gccgtcaagg
      541 acgcccgatc cgacactgcc gccaagaagt tggccgcgtc gacacggccg gccctgaccg
      601 tcagcacggt cgcgccgcag aaggccgaat ggccgcgcac gatctccgcc aacggcaata
      661 tcaccgcctg gcaggaagcc atcatcggcg ccgagttgtc ggggctgcgg ctcaccgacg
      721 tgctcgtcaa cgtcggcgac acggtcaagc gtggccagct gctcgcacgc atcgccgccg
      781 acgccgtgtc agccgacctc gcgcaggcgc gcgcgtcggt ggtcgaggcc gaggcgtcac
      841 tggctgaagc caaggccaac gcccagcgct cgaagcagtt gcaggcacag ggcttcatca
      901 gcccgcaggc gacgattcag gtcgttaccg cggagcagac cacggctgca cgcatcgcgg
      961 cggcgcgtgc gcgtgtccag gctgaggacg tgcggctggc gcagacgcgc atcgttgctc
     1021 cggacgacgg agtgatctct gcacgcacgg ccacggtcgg gtcgctgacg cagcccaacc
     1081 aggaactgtt ccgcctgatc cgcggcggtc gcctggaatg gcgcgccgaa gtgacggcgg
     1141 ccgaactcgc gcgcttgcag cccggcgttg ccgcgagcct gacgacgccc agtggagaac
     1201 gcatcaccgg ccgcgtgcgc gccgtcgcgc cctcggtcga tccggccacg cgcaacggca
     1261 tcgtctacgt cgacatcgcc gccggcaccg cggcgcgggc cggcaccttt gcgcgcggcg
     1321 agttcgagct cgaccgcagt ccggcactca ccctgccgca gagcgcggtg ctgctgcgcg
     1381 acggctttgc ctacgtgttc cgcatcggac cggacaacag ggtcgcccag atcaaggtcg
     1441 aactgggacg tcgtgtcggc gatcgcatcg aagtgcgcga cggcatcgat gcgacaacgc
     1501 gggtcgtcga aaccggcgcc ggcttccttg ccgacggcga taccgtgcgc gtcgtcgagt
     1561 cgccggccct gccggtcaag tcggcggcga aacagtaaga gtaagaggcc ccgggatgaa
     1621 cgtttccgcc tggtcaatcc gcaatccgat tcccgcggtg ctgctgttcg tcatgctgac
     1681 gctgatcggc gtgctgggct ttcgcggcat gaagatccag cagttccccg acatcgacct
     1741 gcccaccgtg acggtggtcg cggcgcttcc aggtgccgcg cccgcgcaga tggagaccga
     1801 ggtcgcccgc aagatcgaaa attcgatcgc gacgctgcag ggcatcaagc acatttacac
     1861 caaggtgcag gacggcaccg cgacgatcac cgtcgagttc cgcctggaaa agccgacaca
     1921 ggaggcgctc gatgacgtgc gtgatgcagt ctcgcgcgta cgctccgacc tgccgggcga
     1981 cctgaaggac ccggtcgtgt ccaaggtcaa cctggccggt tcgccgatcc tgacctacac
     2041 ggtcgcctcg ccacgcatgg acgacgaggc actgtcgtgg ttcgtcgaca acacggtaac
     2101 caagaacatg ctgagcgtgc gcggcgtcgg cgccgtggct cgcgtcggcg gcgtcaatcg
     2161 cgagatccgc gtcgaactcg atccggcgcg attgctggcg ctgaaggcga cagcagccga
     2221 catctcgcgg cagctgcgac agatccagca ggaagcctcc gggggccgcg ccgacatcgg
     2281 tggtatcgaa cagtcggtgc ggaccatcgc gacggtccag tcagccgagg aactggcggc
     2341 catggacatc gcactcgccg acggccgccg catccgcctc gaccaggttg cgacggtgtc
     2401 ggacaccgtc ggcgaacagc gttcggccgc actgctgaac ggcatgccgg tggtcggctt
     2461 cgagatcacg cgcagtcgcg gcgccggcga ggtcgacgtc gccgaggggg tgcgcgcgca
     2521 gcttgcgcaa ctcaagagcg agcacccgga catccagatc accgaggcat tcaacttcgt
     2581 cgatcccgtg gaggaaaact tcaccggatc gatgtggctg ctgatcgaag gtgccctgct
     2641 ggccgttctg gtcgtgtggc tcttcctgcg cgactggcgc gcgacgctgg tgtcggccgc
     2701 tgcgctgccg ctgtcgatcc tgccgacgtt cggcgcgatg tacctgatgg gcttcacgct
     2761 gaacgtcgtg acgctgctga cgctgtcgct ggtggtcggc atactggtcg acgacgcgat
     2821 cgtcgagata gagaacatcg tgcgccacat gcgcatgggc aagtcgccgt accaggcgtc
     2881 gatggaagcc gccgacgaga tcggactcgc ggtcatcgcc acgacgttta cgctgatcgc
     2941 ggtgttcctg ccgacggcgt tcatgagcgg cgtgccgggc aagttcttcg tgcagttcgg
     3001 ctggaccgcg gcgatcgcgg tgttcttctc gctcgtggtc gcacggatgc tcacgccgat
     3061 gatggccgcg tacctgctca ggccaccgcg caaggaccat gctgagccgc gctggatgac
     3121 gatctacctg cgctgggcgc agtggtgcct gaagcatcgc atcgcgacct cgctggccgc
     3181 cgcggcgttc ttcttcggct cgttcgcgct ggtgccgctg ctgccgacgg gtttccttcc
     3241 gcccgacgac ctgtcgcaga cccaggtcta cctgacgctg ccgccgggca gcaccttcaa
     3301 tcagacactg gcaacggccg aacaggcgcg cgccatcgtc gagaagcatc cacacgtgaa
     3361 gctggtctac accgccatcg gtggcggcgc cgccgggtcc gacccgttcg cgccgcgcgg
     3421 tgctgcggaa gcccgcaagg cgacactgac aatcaacctg accccacgca gtgatcgccg
     3481 tggcatctcc aagcagtccg tcgagcgcga a
//
LOCUS       DATIRT010000399         2117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1838209, whole genome shotgun sequence.
ACCESSION   DATIRT010000399 DATIRT010000000
VERSION     DATIRT010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1838209"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VLN25_08980"
     CDS             complement(<1..467)
                     /locus_tag="VLN25_08980"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HSQ10735.1"
                     /translation="MSSVVRSRWGRRVLIALAMAAVVAMAVWWLGRPKPIPVVLTEIG
                     RGRVEATIANTRAGTVEACQRTKLSTIIGGRIEVLTVKEGDRVRKGQMLMQLWNDDQQ
                     AAEWLARAQLDTSRKRVVEACTIAAGAEREVARQVELHKQGFISANAIDSARAA"
     gene            complement(666..977)
                     /locus_tag="VLN25_08985"
     CDS             complement(666..977)
                     /locus_tag="VLN25_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10736.1"
                     /translation="MSFWSALTRKTFGSNDRVVAHILDPITGYSNRDLQVGKDIDADT
                     VRRLGDNGAVFVVVAYDGGTPTATFCRRAQWLRAQAQQHAADRDVDASVWGKRQELEI
                     R"
     gene            complement(1202..1765)
                     /locus_tag="VLN25_08990"
     CDS             complement(1202..1765)
                     /locus_tag="VLN25_08990"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HSQ10737.1"
                     /translation="MIEATIELMRGSGLSGAGINEIVRESAAPKGSVYHFFPAGKQQI
                     VSEALVDYAGRVRQFIDAALSSRREPAQKVRALFDVFAQRVEAGQFRRSCAFGAVCLD
                     LDADLDELRSVIAEAFDDWQVLIAGHFDLGDASRTRSLAGLLLTAIEGAYIRARAERS
                     SRPFREAGAWLGELAIPRSGTARATPR"
     gene            1926..>2117
                     /locus_tag="VLN25_08995"
     CDS             1926..>2117
                     /locus_tag="VLN25_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HSQ10738.1"
                     /translation="MNDSALDIRATVSAEEWQQRVDLAACYRLVAMFGWDDLIFTHIS
                     ARLPGPDHQFLINPYGMMFD"
BASE COUNT          376 a          726 c          643 g          372 t
ORIGIN      
        1 gccgcgcgcg cgctgtcgat cgcgttggca gagatgaatc cctgtttgtg cagctccacc
       61 tgccgcgcca cctcgcgctc ggcgccggcg gcgatcgtgc aggcctcgac cacacgcttg
      121 cgcgaggtat cgagctgggc ccgcgcgagc cactcggccg cctgctgatc gtcgttccac
      181 agctgcatca gcatctggcc ctttctgacg cggtcgcctt ccttgactgt caacacctcg
      241 atccgtccgc cgatgatggt cgacagcttg gtgcgctggc acgcttcgac ggtgccggcc
      301 cgcgtattcg cgatcgtcgc ttcgacgcga ccgcgtccga tctcggtcag caccaccgga
      361 atcggcttgg ggcggcccag ccaccagacg gccatcgcga cgaccgccgc catcgcgagc
      421 gcgatcagta cgcgacggcc ccagcgcgat cgaactacgg aactcatcag agtgactggc
      481 ttgagtaacg aactagcgga gattagccga cgcggcggca gtgagatgcc agaccgttag
      541 atctgccagg cctcggacag ccgccccatc gggtgcggca accggccgag gttgactcgc
      601 gcatcggcgt cacttactta ccggcacagg gatactggcc cgacgcgaag tcacttcagg
      661 cagcgctagc gaatctcaag ttcttgccgc ttgccccaga ccgacgcgtc gacgtcccga
      721 tcggcggcgt gttgctgcgc ttgagctcga agccattgtg cccgcctgca gaaggttgct
      781 gtgggagtgc ccccgtcata cgcaacaacg acgaacacgg ccccgttgtc acccaagcgc
      841 cggacggtgt cggcatcaat gtccttgcct acctgcaggt cccggttcga gtagcccgta
      901 atcggatcga ggatgtgggc gaccacgcga tcgttcgatc cgaacgtctt tcgcgttagc
      961 gcactccaaa agctcatgtc gggttcccaa ccttgcacag gtgacggccg atggtattcg
     1021 gtcgccgatg gcattagctg atctagcgca gcaaccgatc gtgacgcggc cagacgctcc
     1081 gagccgacgt gacatttgac gctcccggcc aaggaacgag cactatcgca cagcgaaaga
     1141 acggtggttc cgcggaacga tcgcgtggac cagtctcgct ggccgaggtc acccttcagc
     1201 cctatcgcgg ggttgccctg gccgtgccgg acctgggaat cgccagctcg ccgagccagg
     1261 cgcccgcttc gcggaaaggt cggcttgacc gttcggcacg cgcccggatg tacgcacctt
     1321 cgatcgccgt gagcaggagt ccggctaacg agcgggtgcg actggcatcg ccaaggtcga
     1381 agtgacctgc gatcagcacc tgccagtcgt cgaacgcttc ggcgatgacg gagcgtaact
     1441 cgtcgaggtc ggcgtccagg tcgaggcaga cggcgccgaa ggcgcagctg cggcggaact
     1501 gtccggcctc gacccgctgc gcaaacacgt cgaacagcgc cctcaccttc tgcgccggct
     1561 cccggcgtga tgacagcgcc gcgtcgatga actgccgcac acgcccggcg tagtcaacca
     1621 acgcctccga aacgatctgc tgcttgccgg cgggaaagaa gtggtagacg gagcccttcg
     1681 gcgctgcgct ttcgcggacg atctcgttga tgccggcgcc actcagtccc gagccgcgca
     1741 tcaactcgat cgtcgcctcg atcatgcgct gccggctgcc gggcgccgcc accgtcctgt
     1801 ggcggccctc agaccgggca gatcggctga tgcttccgga ttccttggac accccgactc
     1861 cttgaccgat taactagacc agtctatata ttagcgcacc atccatcgac cgacaagaca
     1921 gatacatgaa cgattccgcc ctcgacatac gtgcgaccgt cagtgccgaa gaatggcagc
     1981 agcgcgtcga cctcgccgcc tgctaccgct tggtggcgat gtttggctgg gacgacctca
     2041 tctttaccca catctcggcg cgcctgccgg ggccagacca ccagttcctg atcaatccct
     2101 acggcatgat gttcgac
//
LOCUS       DATIRT010000400         3081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_363310, whole genome shotgun sequence.
ACCESSION   DATIRT010000400 DATIRT010000000
VERSION     DATIRT010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363310"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..702
                     /locus_tag="VLN25_09000"
     CDS             130..702
                     /locus_tag="VLN25_09000"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HSQ10739.1"
                     /translation="MDLPSLVQQYGLIAVVGGAFLECETIVLLAGAAAHLGLLDLRAV
                     VAAAAFGAFAGDNFFFFLGRRYGADVTNRYAWMAAAVPRIDRMLSRWRWGAVVALRFM
                     YGLRTAGPMLIGAGGMPALEFVTANALGALLWAMLIGGIGFVGGRAVETLLGDIAHAE
                     KIVLAVAVAIGVLAIVIRSLLRRRHAAPKS"
     gene            771..1430
                     /locus_tag="VLN25_09005"
     CDS             771..1430
                     /locus_tag="VLN25_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSQ10740.1"
                     /translation="MRILLVEDDPMIGESVADGLKSEGYAVDWVRDGKEAELAVAASP
                     YSLVILDLGLPRRDGIDVLKSIRERRLGVPVLVMTARDTVRDRIKGLDAGADDYLIKP
                     FDLDELTARARALIRRAAGRAEPLIERGALAINPATREVSWRGEPVALSAREYALLAA
                     LAERAGLVLSRSQLEEKLYGWNEAVGSNAVEVHIHNVRKKLGDSVIRNVRGMGYTLAT
                     E"
     gene            1492..2826
                     /locus_tag="VLN25_09010"
     CDS             1492..2826
                     /locus_tag="VLN25_09010"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSQ10741.1"
                     /translation="MTSIRRWLLAALLAALAVAGLAAAAATYFSARGEVDTLLDEELR
                     QVALSLRDHAVLDLRRLESVAANPDQRVLVQIWDPAFDRPYVSRIVGSGPMPRTDEGY
                     ATILHDGRPWRAFTLFTGRHLIQVAQPTAVRTELAARTAWRLLLPVLVLLPFLGLAGW
                     WIVGRGLAPLGRIAGAVKERSPTALTPVSTSGMPVEIQPLADALNDLLGRLDQSFSLQ
                     RRFAADAAHELRTPLTALSLQVQLAERAQTDEDRARAFERLRQGIRRATRLVQQLLTM
                     ARLDPDAAAATPTAVDVAALVASVVEDARPLAAERSVDLSSHDGSGPSTVSGSEDALR
                     ILLNNLIDNAVRYTPGGGRVTTSVMRDADTVEVLVEDTGPGIPDEERERVFDRFYRGQ
                     NAGASGTGLGLAIVSQVAEMHRGRVALEASEGGGLRVRVRLPLAAAESGKTG"
BASE COUNT          474 a         1023 c         1049 g          535 t
ORIGIN      
        1 ccatcggccg cgaacgcggc acgccgcgcg cctgagcggg gccaccgtgc cgtcgtgcgt
       61 gtacgcgagg tagtctctcg accgggctgc atgcgctcat tctgagcgag ccgcgggcaa
      121 agaaaactca tggaccttcc ctcgctcgtc cagcaatacg gcctgatcgc cgtcgtgggc
      181 ggggcgttcc tcgaatgcga gaccatcgtg ctgctggccg gtgcagctgc gcacctggga
      241 ctactggacc tgcgagcggt cgtcgctgcc gccgctttcg gcgcgttcgc gggagacaat
      301 ttcttcttct tcctgggccg gcgctatggg gcagacgtca cgaaccgcta cgcgtggatg
      361 gcagccgcgg tcccgcgaat tgatcggatg ttgtcgcgct ggcgctgggg cgccgtggtc
      421 gccctgcgct tcatgtacgg cttgcggacg gctgggccga tgctgatcgg ggcgggcggc
      481 atgccggccc ttgagttcgt tacggccaac gcgctcggcg cgttgctgtg ggcaatgctg
      541 atcggcggca tcggctttgt cggcggccgg gcagtggaga cattgctggg tgacatcgcc
      601 cacgccgaga agatcgtgct cgcggtggcg gtggccatcg gcgtgcttgc gatcgtcatt
      661 cgaagcctgc tgcgacgacg acacgccgcc ccgaagtcct gaggcgctac cgcctgcagc
      721 gacgcgctat cgttgcggtc actttacttc catgccttcg accctgcgtc atgcgcatcc
      781 tgcttgttga agacgaccca atgatcggcg aaagcgttgc cgacggcctc aagtccgagg
      841 gctatgcggt cgactgggtc cgcgacggga aggaagcgga gctcgccgta gcagcgtcgc
      901 cgtattcact cgtgatcctc gacctcgggt tgccgaggcg cgacggtatc gatgtgctga
      961 aaagcatccg cgagcgccgc ctcggcgtgc ctgtgctggt gatgacggcg cgcgacaccg
     1021 tgcgcgaccg gatcaaggga ctcgacgcgg gcgcagatga ctacctgatc aaaccgttcg
     1081 atctcgacga actcacggcg cgcgcacgcg ccctgatccg ccgggccgcg ggacgcgcgg
     1141 agccgttgat cgaacgcggt gcactcgcga tcaatcccgc gacgcgcgag gtgagctggc
     1201 gcggcgagcc cgtggcactt tccgcgcgcg agtacgcctt gctggcagcc ttggccgagc
     1261 gcgccgggct ggtgctgtcg cgttcgcagc tggaagaaaa gctctacgga tggaacgaag
     1321 cggtgggcag caacgcggtc gaggttcaca tccacaacgt gcgcaaaaaa ctgggcgaca
     1381 gcgtcatccg caatgtgcgc gggatgggct acacgctcgc gaccgaatga gtctcagcgc
     1441 cgcgtcgttg tgccgccgcg cccgtgaggt caggcacgtg aggtcgagca cgtgacatcg
     1501 attcgtcgtt ggctgctcgc ggccctgctg gccgcgcttg ccgtggccgg cctggccgcg
     1561 gccgcggcga cgtacttttc agcgcgcggg gaagtcgaca cgctgctcga cgaagagttg
     1621 cggcaggtcg cattgtcgct gcgtgatcac gcggtgctgg acctgcgccg gctcgagagc
     1681 gtcgccgcga atcccgacca gcgcgtgctg gtgcaaatct gggaccccgc attcgatcgg
     1741 ccatatgtct cgcgcatcgt cgggtcgggt cccatgcccc gcaccgacga aggctatgca
     1801 acgatcctgc acgacgggcg accctggcgc gcgttcacgc tattcaccgg ccgccacctg
     1861 atccaggtcg cgcagccgac ggcggtgcgc accgagctcg ccgcgcgcac cgcgtggcgc
     1921 ctgctgttgc cggtgctcgt gctgctgccg ttcctcggac tcgccggctg gtggatcgtc
     1981 ggtcgcggcc tggctccact cggccgcatt gcgggcgccg tcaaggaacg cagtccaacc
     2041 gcactgacgc cggtatcgac ctccggcatg ccggtcgaga tccagccgct cgcggatgcg
     2101 ctcaatgatc tgctgggccg tctcgatcaa tccttctcgc tgcagcggcg ctttgccgcc
     2161 gatgctgcgc acgaactgcg cacgccgctg acggcgctca gtctccaggt gcagcttgcc
     2221 gagcgcgccc agaccgacga ggaccgggcc cgtgccttcg agcgcctgcg tcagggtatc
     2281 cgccgggcca cccggctggt acagcaactc ctgacgatgg cccgtctcga tccggacgcg
     2341 gcggcggcca ctccgacagc ggtcgatgtc gccgcgctcg tcgcctcggt cgtcgaggac
     2401 gcacgaccgc tggccgccga gcgatccgtc gacctgtcga gccacgatgg cagcgggcca
     2461 tcgaccgtat cgggcagcga agacgcgctg cgcatcctct tgaacaacct gatcgacaac
     2521 gcagtccgct atacgccggg tggcggacgg gtgacgacct cggttatgcg cgatgccgac
     2581 acggtggagg ttctggtgga agacaccggc cccggcatac ccgacgagga acgcgaacgc
     2641 gtgttcgacc gcttctatcg tggtcagaac gccggagcgt ccggcacggg tctgggactg
     2701 gccatcgttt cgcaggttgc ggaaatgcat cgcggccggg tcgcgctcga ggcgagtgaa
     2761 ggcggcggac tgcgggtccg tgtccggctt ccgctggcag ctgccgaaag tggcaagact
     2821 ggatgaggct gcgcagacga tcttcacgcg ctagagcgtc aatcttgaat gcaagtgctc
     2881 gcttacgcgc tgtggcttgg agatggtgcg atcgggacag ctcgcccaac ctggtcgcag
     2941 gcgactgttg atcgatcaca agccgaccga ccaacggcgc cgcccttaag tcagcgttaa
     3001 gtcacagcgc ctagctttcg atccgtggtc gacggccaac ctccgtcgct cctcactcac
     3061 tcgaaatttc aagcgccgcc c
//
LOCUS       DATIRT010000401         2574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_757066, whole genome shotgun sequence.
ACCESSION   DATIRT010000401 DATIRT010000000
VERSION     DATIRT010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757066"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VLN25_09015"
     CDS             complement(<1..746)
                     /locus_tag="VLN25_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSQ10742.1"
                     /translation="MSASDTPQVLGLDKYSAPQWLAARANRRFHVMAKPAGSACNLDC
                     AYCFYLSKRVLPGGPGSGHMPDEVLERFVKDYIDSVTSDEVVFSWQGGEPTLLGLEFF
                     EKVVTLQAKHAKPGQRIENDLQTNGTLLDDDWARFLKQHRFLVGLSIDGPREIQDHCR
                     ITKHAEPTFDQVFAAAKMLQKHGVRFNTLTCVHRYNASRPLDVYRFLRREIGSTYIQF
                     IPIVEIRGFENMAPGTWDEAKLPMLGDPRC"
     gene            complement(872..2110)
                     /locus_tag="VLN25_09020"
     CDS             complement(872..2110)
                     /locus_tag="VLN25_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012338963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HSQ10743.1"
                     /translation="MTQIYVAGIAMTVFGRHPDRSLHDLAGEALTRALADAGCTTRDL
                     GVAYYSGITNGLLQGQISIPGQVVFSKIGVEGIPVYNIENACASGSSSFNLAVQSLNA
                     GTTDVALALGAEKMNIPDKTKAIAIFEGGWDVSQADENAATLVQLGAGITPPPGSESD
                     RPYSKFMAIYAAMCRWHMKTYGTTQRQLAAVCAKNHQHSVHNPWSQFRKPFTIEEVLA
                     APPITYPLTLPKCAPLSDGAAAAILCTEEGLQRIGADRKRCIKVSASVIRSFTHRRMD
                     EPEKHVSRLAALQAYEMAGLGPEDMDVVEVHDASAMGEIIQAENLGLVPLGGGGPAAE
                     RGEFTLGGRLPVNPSGGLESKGHPLGATGIGQLYELVTQLRGEAGARQVEGARHAIQE
                     NGGGLQGIEEAAVAVHILSR"
     gene            complement(2155..>2574)
                     /locus_tag="VLN25_09025"
     CDS             complement(2155..>2574)
                     /locus_tag="VLN25_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012338964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HSQ10744.1"
                     /translation="KTAETIVDGWLHTGDIGHLDADGYLHITDRKKDMIITGGFNVYP
                     SEVEQVIWSHPAVQDCAVIGVPDEHWGEAVTAVVELNAGQQVGADELIVLCKDRLGSV
                     KAPKSIEFVDSLPRSPVGKVLKKDLRAKYWQNVDRRI"
BASE COUNT          409 a          813 c          848 g          504 t
ORIGIN      
        1 tggcagcgcg gatcgccgag catcggcaac ttcgcttcgt cccaggtccc gggagccatg
       61 ttctcgaatc ccctgatctc gacgatcggg atgaactgta tgtacgtcga gcctatctcg
      121 cggcgcagga agcggtatac atcgagcggc cgcgaggcgt tgtagcggtg cacgcaggtc
      181 agcgtgttga agcggacacc gtgcttctgc aacatctttg ctgcagcaaa cacctggtcg
      241 aacgttggtt ccgcgtgctt ggtgatccgg cagtgatctt gaatctcgcg cggaccgtcg
      301 atgctcaggc cgacgagaaa tcggtgctgc ttgagaaacc gggcccagtc gtcgtcgagc
      361 agcgtgccgt tggtctgcag gtcattttcg atccgttgcc ctggcttggc atgcttcgcc
      421 tgcagcgtga ccaccttctc gaagaactcg agcccaagta gcgttggttc gccgccttgc
      481 cacgagaaga cgacttcgtc gctggtgacg ctgtcgatgt agtccttgac gaagcgctcg
      541 aggacctcgt ccggcatgtg gccactgccc ggtccgccgg gcagtacccg cttgctgagg
      601 tagaagcagt acgcgcagtc gaggttacag gccgagcctg cgggtttggc catgacgtgg
      661 aaacgtcgat tggcgcgtgc cgctagccat tgcggggcgc tgtatttgtc gagtcccagg
      721 acctgcggtg tatccgatgc gctcacgcga tgcttcacga gtgaccgaga ggaatgcggt
      781 gcctagtgaa gggcagcgct cagcgctttg ccaccgtatt gctacctagc aaggtagcgt
      841 ccagacctgc agcgccgctt cgcgtccaga ctcaccggct gaggatgtga accgcgactg
      901 cggcctcctc gatcccctgc agcccgccgc cgttttcctg gatggcgtgg cgagcgccct
      961 cgacctggcg tgcaccggcc tcgccacgca gctgcgtaac caactcgtag agctggccga
     1021 tgccggttgc gccgagcgga tgacccttgg actccaggcc gcctgaagga ttgacgggta
     1081 agcgcccgcc gagcgtgaac tccccgcgct cagcagccgg gccgccgccg cccagcggca
     1141 ccagcccgag gttctcggcc tggatgatct cgcccatcgc cgacgcgtcg tgcacctcga
     1201 ccacgtccat gtcctcgggg ccgaggcctg ccatctcata ggcctgcagc gcggccaggc
     1261 ggctcacgtg cttctcgggt tcgtccatgc gccggtgcgt gaagctgcgg atgacgctgg
     1321 ccgacacctt gatgcagcgc ttgcggtcgg cgccgatgcg ctgcaaccct tcttcggtgc
     1381 acaagatggc ggccgccgcg ccatcagaga gcggcgcgca cttcggcagc gtgagcgggt
     1441 aggtgatggg cggcgccgcg agcacttcct cgatcgtgaa gggcttgcgg aactgcgacc
     1501 agggattgtg cacggagtgc tggtgattct tggcgcacac cgcggcgagc tgccgttgcg
     1561 tggtgccgta ggtcttcatg tgccagcggc acatcgccgc gtagatggcc atgaacttgc
     1621 tgtacggccg gtcggactcc gatccgggcg gcggcgtgat gccggcgccg agctgcacca
     1681 acgtggcggc gttctcgtcg gcctgcgaga cgtcccaacc gccctcgaag atggcgatgg
     1741 ccttggtctt gtccgggatg ttcatcttct ctgcgccgag ggcgagtgcc acgtcagtgg
     1801 tgccggcatt gaggctctgc acagcgaggt tgaacgacga gctgcccgaa gcacatgcgt
     1861 tctcaatgtt gtagacggga atgccttcga cgccgatctt gctgaagacc acctggcccg
     1921 ggatcgagat ctggccctgc agcaggccat tggtgatgcc cgagtagtag gcgacgccga
     1981 gatcgcgcgt cgtgcagccg gcgtcggcga gcgcacgtgt cagcgcctcg ccagccaggt
     2041 cgtgcaggct gcggtcggga tggcggccga acaccgtcat ggcgatgccg gcgacataga
     2101 tctgggtcat gggattacct cgcgagtgcc ttgatgatcg tcggacagca gcactcaaat
     2161 cctgcggtcg acgttctgcc agtacttcgc ccgcaggtcc ttcttgagca ccttgccgac
     2221 cgggctgcgt ggcagggagt cgacgaactc gatggacttc ggcgccttca ccgagccaag
     2281 cctgtctttg cacaacacga tcagttcgtc cgccccgact tgctggccgg cgttgagttc
     2341 gacgaccgcc gttacggctt caccccaatg ctcgtcgggc acgccgatca cggcgcagtc
     2401 ctgcactgcg gggtgactcc agatcacctg ctcgacttca cttgggtaca cgttgaagcc
     2461 gccggtgatg atcatgtcct tcttgcgatc ggtgatgtgc aggtagccgt cggcatcgag
     2521 gtggccgatg tcgccggtgt gcagccagcc gtcgacgatg gtctcggcgg tctt
//
LOCUS       DATIRT010000402         2300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_67722, whole genome shotgun sequence.
ACCESSION   DATIRT010000402 DATIRT010000000
VERSION     DATIRT010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67722"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VLN25_09030"
     CDS             complement(<1..359)
                     /locus_tag="VLN25_09030"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSQ10745.1"
                     /translation="MAAELQAFYEANPEYYQITHGHAPAPDAALQGFDFRPPADMSYT
                     ALPMWLIRERDSGRVVGEVSVATDLLAAGVIHLGFFMVATSRHGTGFAADVYESYEAW
                     AIGQGARWLRLGVVEGH"
     gene            complement(410..868)
                     /locus_tag="VLN25_09035"
     CDS             complement(410..868)
                     /locus_tag="VLN25_09035"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSQ10746.1"
                     /translation="MTMTVDLREVTKDTVSSVCKLDAGDDGKQVAPNSVSIAQAYFSP
                     EAWFRAVHAGDELVGFVMLYDHTLAEAPEEREFFLWRLMIDRRHQGKGYGHLAVERLV
                     AHVRTRPAAERLLVSHVKDADRLARFYESLGFRYTGKEDEGELVMARDLS"
     gene            937..2031
                     /gene="ychF"
                     /locus_tag="VLN25_09040"
     CDS             937..2031
                     /gene="ychF"
                     /locus_tag="VLN25_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HSQ10747.1"
                     /translation="MALKCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIEPNIGIV
                     EVPDARLMALASIANPQKVLSATVEFVDIAGLVAGASKGEGLGNQFLANIRECDAIAH
                     IVRCFEDPNVIHVAGRIDPISDIEVINTELALADLQTVDKQLARYTKIAKSGGDKEAK
                     RLVDALEKILPALNEAKPVRSVDLYDEEKDVLRPLFLLTAKPTMYVANVEEHGFEGNP
                     LLERVREHAAKEGAPVVAVCAKIEAEIADLPDEDKEIFLADMGMHEPGLNRLIRAGYT
                     LLGLHTYFTVGPKEVRAWTITAGSTAPQAAGVIHTDFEKGFIRAQTIAYDDFVAHKGE
                     QGAKEAGKMRAEGKEYVVRDGDVLNFLFNV"
     gene            2038..>2300
                     /locus_tag="VLN25_09045"
     CDS             2038..>2300
                     /locus_tag="VLN25_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HSQ10748.1"
                     /translation="MTASIDQLFIYPIKGCRGISVQHETLTATGLAIDGIGDREWVVV
                     DEHGEFLSQRELPAMALIETRLTSTTLRLKAPGMLQLEVPFAS"
BASE COUNT          455 a          741 c          713 g          391 t
ORIGIN      
        1 tgatgtcctt cgaccacgcc gaggcgcagc caacgcgcgc cttgaccgat tgcccaggct
       61 tcataggact cataaacgtc ggctgcgaaa cccgtgccgt gccgcgacgt tgcaaccatg
      121 aagaagccga ggtggatgac acccgccgcc agcaggtcgg tagccaccga gacctcgccg
      181 accacccgac cggagtcacg ctcgcggatc agccacatcg gcagagcggt gtacgacatg
      241 tcggcgggcg gcctgaagtc gaatccctgc aaggcggcat cgggcgcggg cgcatgcccg
      301 tgcgtgattt ggtagtactc tggattcgcc tcgtagaacg cctggagttc ggcggccatg
      361 gatcgatcgg cctctgtagc gatccagtcc gcactgggcc gcagcggact caactcaggt
      421 cccgcgccat gaccagctcg ccctcgtcct ccttgccggt gtagcggaaa ccgagcgatt
      481 cgtaaaagcg cgcgagacgg tcggcgtcct tgacgtgcga caccagcagc cgttcggcgg
      541 ctggacgggt acgcacatgt gcgaccagac gctcgaccgc gagatgccca tagcccttgc
      601 cctggtggcg gcggtcgatc atcagccgcc acaggaagaa ctcgcgttcc tcgggcgctt
      661 cggccagcgt gtggtcgtag agcatcacga agccgaccag ctcgtcaccg gcatgaacgg
      721 cccggaacca cgcctccggc gaaaagtagg cctgcgcaat cgacaccgag ttcggcgcga
      781 cctgcttacc gtcgtcgccg gcatcgagct tgcacacgga ggacacggtg tccttcgtca
      841 cttccctgag atcgacagtc atggtcattg ggaaagtctt caaaggcttg cgataattgc
      901 acgctttcgc ccgcgcggcg caccggacac ctcccaatgg ccctgaaatg cggcatcgtc
      961 ggcttgccca atgtaggcaa atcgacgctc ttcaatgcac tcacgaaggc cggcattgcg
     1021 gccgagaact atccgttctg cacgatcgag cccaacatcg gcatcgtcga ggtgcccgac
     1081 gcgcgcttga tggcgctcgc ctcgatcgcg aatccccaga aagtactgtc ggccacggtc
     1141 gagttcgtcg atatcgccgg tctggtggcc ggcgcgagca agggcgaagg cctgggtaat
     1201 cagttcctcg ccaatatccg cgagtgcgat gccattgcgc acatcgtccg atgcttcgag
     1261 gatccgaacg tcatccacgt cgccggcagg attgatccca tctccgatat cgaggtcatc
     1321 aacaccgagt tggccctcgc cgacctgcag acggtcgaca agcagcttgc gcgctacacc
     1381 aagatcgcca agagcggcgg cgacaaggaa gcgaaacgac tggtcgatgc gctcgagaag
     1441 atcctgcccg cgctcaacga agcgaagccg gtccgctcgg tcgatcttta cgatgaagag
     1501 aaggacgtgc tgcggccgtt gtttctgctg accgccaagc cgacgatgta cgtcgccaac
     1561 gtggaagagc atggtttcga gggcaatcct ctgctcgaac gcgttcgcga gcatgccgcg
     1621 aaggagggag caccggtcgt ggcggtgtgc gccaagatcg aagccgagat cgccgacctg
     1681 cccgacgagg acaaggagat cttcctggcc gacatgggca tgcacgaacc cggcctgaac
     1741 cggttgatcc gcgccggcta caccttgctg ggcctgcata cctacttcac ggtcggcccc
     1801 aaggaagtgc gcgcgtggac catcaccgcc ggcagcacgg caccgcaggc ggccggcgtg
     1861 atccacaccg atttcgaaaa gggtttcatc cgcgcgcaga ccatcgccta cgatgatttc
     1921 gtcgcccaca agggcgaaca gggcgccaag gaagccggca agatgcgtgc ggaaggcaag
     1981 gagtacgttg tgcgcgacgg tgatgtactg aacttcctgt tcaatgtctg acgcgctatg
     2041 accgcctcca ttgatcagtt gttcatctac ccgatcaagg gctgccgcgg catctctgtc
     2101 cagcacgaga cgctgacggc gaccggactt gcgatcgatg gcatcggtga ccgcgagtgg
     2161 gtcgtcgtcg acgagcacgg cgagttcctg agtcagcgcg agttgccggc catggcactg
     2221 atcgagacgc gcctgacgtc gacgacgctg cggctcaaag cgcccggcat gctgcagctc
     2281 gaagtcccgt tcgccagcga
//
LOCUS       DATIRT010000403         2554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_363388, whole genome shotgun sequence.
ACCESSION   DATIRT010000403 DATIRT010000000
VERSION     DATIRT010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363388"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VLN25_09050"
     CDS             <1..255
                     /locus_tag="VLN25_09050"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HSQ10749.1"
                     /translation="LKEKLLAAHRGGIKTVLIPEENVKDLAEIPDNVKNRLEIVPVKW
                     IDKVLEVALERQPVPLTDDEAAAAPPKPADAGTGTEVVKH"
     gene            477..749
                     /locus_tag="VLN25_09055"
     CDS             477..749
                     /locus_tag="VLN25_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HSQ10750.1"
                     /translation="MNKTELIDHIAKQADISKAAAGRALEAIVGGIRGSLRKGNSVTL
                     VGFGTFTVGKRAARTGRNPRTNEAIKIKAAKVPKFRPGKALKDSLN"
     gene            858..933
                     /locus_tag="VLN25_09060"
     tRNA            858..933
                     /locus_tag="VLN25_09060"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:891..893,aa:Val,seq:tac)
     gene            977..1053
                     /locus_tag="VLN25_09065"
     tRNA            977..1053
                     /locus_tag="VLN25_09065"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1011..1013,aa:Asp,seq:gtc)
     gene            1153..>2554
                     /locus_tag="VLN25_09070"
     CDS             1153..>2554
                     /locus_tag="VLN25_09070"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HSQ10751.1"
                     /translation="MFDSIRSHRRWLMFFMLVLIFPSFVFFGIQGYNSFIEQEGALAT
                     VDGMSITQRDFEVAQRDRVERLRQQFGAEFDPKVLDTQEGRASILDGLVIDRALTREV
                     EKGNLVVTNDRLREVIAGIPAFQEDGKFNIERYRAYVTAQGMTEPQFEERVRADLRKQ
                     MLVQAVLESAIVPTQVAERIDRVLLETREVRVLPIRADAFLAKVAVTDAQVAEFYEKN
                     RKDFETPESVKVEYLVLSADAIAGAAPVAEADVKAYYEQNKGRYGVEEQRRASHILIT
                     PEGGDKAAARKKAEGILAALKAAPNDFAKIAREQSKDPGSAAQGGDLGFFGKGMMVKP
                     FEDTAFSLKAGETSEIVESDFGLHIIRVTEIKLAQAKPLDEVRADIEKELRTQQAQKR
                     FAEAAEQFTNLVYEQADSLQPAADTLALKVATQDNLTRAGMPVVKDQPQVFTPRVIEA
                     LFSEDSLKNRRNTQAIE"
BASE COUNT          537 a          778 c          815 g          424 t
ORIGIN      
        1 ctgaaggaaa agctgctcgc agcccatcgc ggcggcatca agacggtgtt gatcccggag
       61 gaaaacgtca aggatctcgc ggagatcccg gacaacgtga agaaccggct tgagatcgtc
      121 cctgtcaagt ggatcgacaa ggtcctcgag gtagccctcg aacgccagcc tgtcccgctc
      181 accgatgacg aggctgcggc ggctccgccg aagcctgccg acgcaggaac cggcaccgaa
      241 gtcgtcaagc attgacgcaa attcagtgtg cgcccgcgcc gcgggcgcac attgtccatc
      301 gcattgctcg gctcagagca aaaaatagtt tgcttctgct tgacactgct atacgagcga
      361 tgttgaatag ccttccactt ttccgaagcg cgccctcggc cgacaaaaag aaaaacatcg
      421 ctgcgacggc tgccacgcgc ggacatctcg aagaccagtc aaaagagggg gtttaagtga
      481 acaagacaga actgatcgac cacatcgcca aacaagcaga catttcaaag gcggcggcag
      541 gtcgagccct cgaagcgatc gtcggtggca tccgcggcag cctgcgcaag ggtaactcgg
      601 tgacgctcgt cggtttcggg acgttcaccg tcggcaagcg cgcagcgcgc accggacgca
      661 atccgcgcac caatgaggcg atcaagatca aggctgcgaa agtgccgaag ttccgtcctg
      721 gcaaggccct caaagattcg ctaaactagc cggcttcgca gccaggcagc cgtcgcagca
      781 aggtcgttcc cagtagccac cgtcattgcc ggagcagggt ttcgctccag ttagattgtg
      841 gaagtacggg cgcgctgggg tgcttagctc agctggtaga gcggcgccct tacaaggcgt
      901 aggtcgggag ttcgagcctc tcagcaccca ccaccgagcg acttggtgaa aatgcgaagc
      961 aatggcagtt cgcgcgggag tggtagttca gttggttaga ataccggcct gtcacgccgg
     1021 gggtcgcggg ttcgagtccc gtccactccg ccacacgacg aaggcgaacg cgagttcgcc
     1081 ttttttgttg ttcgtccagc acaccggact gttgaagttc caatgaagca cgactgaggc
     1141 acgagtccac ccatgttcga ttcgatccgt tcgcatcgtc gctggctgat gttcttcatg
     1201 ctggtgctga tcttcccttc gttcgtcttc ttcggcatcc agggctacaa cagcttcatc
     1261 gagcaggaag gcgcgctggc tacggtcgac ggcatgtcga tcacgcagcg cgacttcgag
     1321 gtcgcgcaac gtgaccgggt cgagcgcctg cgccagcagt tcggggccga atttgatccg
     1381 aaggtgctgg acacgcagga aggccgcgcc tcgattctcg acgggctcgt gatcgaccgg
     1441 gcgctcacgc gcgaggtcga aaagggcaac ctggtcgtga ccaacgatcg cctgcgcgag
     1501 gtcatcgccg gtattccggc gttccaggaa gacggcaagt tcaacattga gcgctatcgc
     1561 gcctacgtca cggcgcaggg gatgaccgag ccccagttcg aggagcgcgt gcgggccgac
     1621 ctgcgcaagc agatgctggt gcaggccgtg ctcgagtcgg cgatcgtgcc gacccaggtg
     1681 gccgagcgca tcgaccgcgt tctgctggag acccgcgaag tacgggtcct tccgatccgc
     1741 gcggacgcgt tcctggcaaa ggtcgcggtc acggacgcgc aggtcgccga gttctatgag
     1801 aagaaccgca aggacttcga gacccccgag agcgtcaagg tcgagtacct cgtgctcagt
     1861 gctgatgcca tcgcaggcgc cgcgccggtg gccgaggccg acgtcaaagc gtactacgag
     1921 cagaacaagg gtcgctatgg cgtcgaggag cagcggcgtg cgagtcacat cctgatcacc
     1981 cccgagggcg gcgacaaggc ggcggctcgc aagaaggccg agggcatcct ggccgcgctc
     2041 aaggccgcgc cgaacgactt tgcgaagatc gcccgggagc agtcgaagga tccggggtcg
     2101 gcggcacagg gcggcgatct gggattcttt ggcaagggca tgatggtcaa gcccttcgag
     2161 gatacggctt tcagcctgaa ggctggcgaa acgagcgaga tcgtcgagtc cgacttcggc
     2221 ttgcacatca tccgcgtcac ggagatcaag ctggcccaag ccaagccgct cgacgaagtt
     2281 cgcgccgaca tcgagaagga actgcgcact cagcaggcgc agaagcgttt tgccgaagcg
     2341 gccgagcagt tcaccaacct ggtctacgag caagccgact cgctgcagcc ggcagccgac
     2401 acgctcgccc tgaaggtcgc gacgcaggac aacctgaccc gcgccggaat gccggtcgtc
     2461 aaggaccagc cacaggtctt cacgccgcgc gtgatcgagg cgctgttctc ggaagactcg
     2521 ctgaagaacc ggcgcaacac gcaggccatc gaga
//
LOCUS       DATIRT010000404         4570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_658648, whole genome shotgun sequence.
ACCESSION   DATIRT010000404 DATIRT010000000
VERSION     DATIRT010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658648"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="VLN25_09075"
     CDS             complement(<1..1031)
                     /locus_tag="VLN25_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSQ10752.1"
                     /translation="MAIDILDELAQLIEHERDALLAAWRAQVRALPAARNLDVPTLND
                     HIPHLLAELASALRTGSGETIAQALTDGSPPAHGLQRVKDACDIEEVVAEYNILRGCI
                     HDLGERNGLNMQGRPFHVLNRVLDGAIGAAVQSFAAHKALEVQERREEYLAFVAHDLR
                     TPLSAISLSARVLESLLTQDRSGTPETAQKFKTLNRNVQHLQDLIGKVLLENTNLETE
                     AGVKLERRRFDLWPFVEALIHDLRPVAGTDSTPLINTIPEDFVVYADASLLHRVFQNL
                     IANAISYTPHGEVVIGASLMADGDTAECFVRDNGEGIPEDRRHVVFDKHETDPEKQGG
                     LGLGLAIVKT"
     gene            complement(1224..2501)
                     /gene="pfp"
                     /locus_tag="VLN25_09080"
     CDS             complement(1224..2501)
                     /gene="pfp"
                     /locus_tag="VLN25_09080"
                     /inference="COORDINATES: protein motif:HMM:NF041103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HSQ10753.1"
                     /translation="MNQHLAILVGGGPAPGINSVIASATIRAVLEGVEVIGIRDGFEW
                     IMKGDIAHTTPLTTDEVAHIHFRGGSHIGISRANPTKDAKHLENTVESLLRLNVNRLI
                     TIGGDDTAYSAMRLEQVAAGRLQIVHVPKTIDNDLDLPAYVDTFGFQTARHHGVEIVK
                     NLMADAKTTSRWYFVIAMGRTAGHLALGIGKAAGATLTLIREEFQAERIRFETIVDTL
                     AGAMIKRLSDGRRDGLAVIAEGLVLGLDPEFLASLPEIERDAHGNVRISEVKIGDLLK
                     AAVQERLKKLGIDMTITAKDIGYELRCADPIPFDLEYTRDLGYCATKYLLSGGNAAMI
                     SIQAGNFVPVPFLELLDPATGRARIRLVDIRSPRYAIARRYMIRLRRDDFEEPLRLTK
                     IAATAGMSVAQFRNEFESLTASELPPLVFGVET"
     gene            2827..3852
                     /locus_tag="VLN25_09085"
     CDS             2827..3852
                     /locus_tag="VLN25_09085"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HSQ10754.1"
                     /translation="MDDQSELQATVGALVQKGKGILAADESSPTIAKRFKSIGVESTE
                     ENRRAYRLMLLATPEFGEFISGVILYEETLGQRADDGTPIPQWAARQGIVPGIKVDAG
                     KIPLAHALGDEITEGLDGLAKRLDGYKQQGARFAKWRAVYNVSDTLPSRVAIESNAEA
                     LARYAAICQERGVVPIVEPEVLIDGNHSIERCAVVTEAVLHEVFHALHRHKVLLEYTL
                     LKPSMVVPGKEHTQQVAPPEVAANTIRILRRTVPAAVPGIFFLSGGQTPAEATANLDA
                     INRLGAQPWQLSFSYGRALQDPALKAWKGQAGNVHETQKALLKRARLNGAAREGKYFP
                     SLEDSLG"
     gene            complement(3841..>4570)
                     /locus_tag="VLN25_09090"
     CDS             complement(3841..>4570)
                     /locus_tag="VLN25_09090"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase 36 associated protein"
                     /protein_id="HSQ10755.1"
                     /translation="WALISGAGEPARARRAMESVGEHLVKPGDSMVLLFAPPFDRSAQ
                     EPGYIKGYLPGVRENGAQYTQAAVWCVIAHALQGDGERAAELFEMLNPINHSSTRAGI
                     HRYKAEPFVIAADVYSEPPHTGRGGWSWYTGAAGWMYRAGIEFILGFKLRGREILIDP
                     CIPEQWRSFKIRYRAPRSTYEFVIDNPQGVARGVVRVEFDGKELAGAAVPLLDDAGVH
                     SVRVTLGPVEYIPGSVAVALDSAE"
BASE COUNT          895 a         1379 c         1438 g          858 t
ORIGIN      
        1 gtcttgacga tggcgagacc cagtcccaag ccaccctgct tctcggggtc ggtttcgtgc
       61 ttgtcgaaca cgacgtggcg gcggtcctcc ggtatgccct cgccattgtc gcggacgaaa
      121 cactcggcgg tgtcgccatc ggccatcaag ctggccccga tgacgacctc cccgtgcggc
      181 gtgtaggaga tggcgttggc gatcaggttc tggaatacac ggtgcagcaa gctggcatcg
      241 gcgtacacga cgaaatcctc gggaattgtg ttgatcagcg gcgtcgagtc tgtcccggcg
      301 accgggcgga gatcatgaat gagggcttca acaaacggcc acaggtcaaa tcgacggcgt
      361 tccaacttga cgccagcctc ggtctccagg ttcgtgttct ccaggagcac cttcccgatc
      421 aaatcctgaa ggtgctgcac gttccggtta agcgtcttga acttctgggc cgtctcgggc
      481 gttccggaac gatcttgcgt gaggagcgat tcgagcaccc gcgcggaaag tgaaatcgcg
      541 ctcagtggcg tgcgaaggtc gtgcgccacg aatgccaaat actcctcccg tcgctcttgt
      601 acttcgagcg ctttgtgggc agcgaacgac tgcacggccg ccccgatcgc tccatccagg
      661 acacgattga ggacatggaa cggcctcccc tgcatgttca gaccgttccg ttctcccagg
      721 tcgtggatac agccccgcag gatgttgtac tcggcaacga cctcttcgat gtcgcaagcg
      781 tcctttaccc gctgcaagcc gtgggcgggc ggactgccgt cggtaagggc ttgagcgatg
      841 gtctcgcccg atccggttcg cagggcgctt gcaagctccg ccaggaggtg cgggatgtga
      901 tcgttgagtg ttggcacgtc gagattccgt gctgcgggca gcgcccggac ttgagcccgc
      961 cacgcagcga gcaaggcgtc gcgttcgtgc tcgatgagct gtgcaagttc gtcgaggatg
     1021 tcgatagcca cctagcctcc gctgatgcga tttgttgaga cggcctcttc cgccgaacag
     1081 ttgagctgag ttgcctgcga aggcaagggg ggcagcggtg cgccgcgacc ccaagtcgcc
     1141 gcactcggaa agttaagcac agcgcgcggc atccgcgcaa tgaagtcaac aacgattggg
     1201 gaaagaggtg ccatcggggg caactaagtc tcgacaccga agacaagcgg cggcaactcg
     1261 gaggcagtca gggattcaaa ttcatttcgg aactgggcga ccgacattcc ggcggttgcg
     1321 gcgatcttcg tgagcctgag cggctcctca aagtcgtccc gccgtagtcg aatcatgtaa
     1381 cggcgggcaa tcgcgtagcg tggggaacga atgtcgacca aacggatcct ggcgcggccc
     1441 gttgccgggt cgaggagttc aaggaacgga accgggacga aattgcccgc ctggatcgaa
     1501 atcattgcgg cgttcccgcc cgagaggagg tacttcgttg cgcagtagcc gaggtcgcgc
     1561 gtgtactcca ggtcgaaggg aatcggatcg gcgcacctga gctcatagcc gatatccttc
     1621 gcggtgatcg tcatgtcgat gccaagcttc ttcaagcgct cctggacggc ggctttcagg
     1681 agatcgccaa ttttcacctc cgagatccgg acgtttccgt gtgcgtcacg ctcgatctcc
     1741 gggaggctcg cgagaaactc cggatcgagg ccgaggacga ggccctccgc gataaccgcc
     1801 agtccatcgc gacgtccgtc ggaaagtcgc ttgatcatgg cgcctgcgag cgtgtcgacg
     1861 atcgtctcga agcgtatccg ctccgcctga aattcttccc ggatcagggt cagcgtggcg
     1921 ccggcggcct tgccgatgcc gagtgcgagg tgccctgccg tccgtcccat cgcaatgacg
     1981 aagtaccagc gcgacgtcgt cttcgcgtcg gccatgaggt tcttgacaat ctcgacgccg
     2041 tggtgccgcg cggtctggaa tccaaacgtg tcgacatacg ccggcaagtc gaggtcgtta
     2101 tcgatcgtct tggggacatg gacaatctgc aggcgaccgg cagccacctg ctcgagcctc
     2161 atggccgagt aggccgtgtc atcaccgccg atcgtgatga gtcggttgac gttgagccgc
     2221 agcagggatt cgacggtgtt ttcgaggtgc ttcgcgtcct tcgtggggtt cgcgcgcgag
     2281 atgccgatat gcgagccgcc gcgaaagtgg atgtgtgcga cttcatcggt cgtgagtggc
     2341 gtggtgtggg ctatgtcgcc cttcatgatc cactcgaagc cgtcgcgaat cccgatgact
     2401 tcaacacctt ccaggaccgc acggattgtc gcggaggcga tgacgctgtt gattccgggg
     2461 gcgggacctc cgccgacgag aatggccaga tgctgattca tgggacgaac ctcacgctga
     2521 gcttacgccg cagggaaaag tgatgaatga aattgatcga caaagcgcca taccaaaact
     2581 cacgcagcgt atctgggtat ctccggacac cctgcctgca gtcaactgac cagaagctcg
     2641 cgcgactgac tcgggaagcg atccctggcc aagatgttcc agatcgcgca atcgggtgag
     2701 gccgcaaacc tgcttataca gacgcgatcc gcgggccgga tctatcctct gctctggcag
     2761 ggtgggtaag ggaccctcag gacgctgcct ctcgccaccc gacccattgc aaaaaaagga
     2821 ctattgatgg acgatcaaag tgaacttcaa gcaacggtag gtgcgctggt tcagaagggc
     2881 aaggggatac tggccgccga tgaaagcagt cccacgattg ccaagcggtt caagtcgatc
     2941 ggcgtcgagt cgacggagga gaatcgtcgt gcctatcgcc tcatgttgct ggcaacgccg
     3001 gagttcggtg aattcatcag cggcgtcatt ctttacgagg agacgctggg tcagcgggct
     3061 gacgacggga ctccgatacc ccagtgggct gcacgccagg gcatcgtgcc ggggatcaag
     3121 gtcgatgccg gcaagattcc actggcccac gcactcggtg acgaaatcac cgaggggctg
     3181 gacggactgg cgaagcgcct tgatggctac aagcagcagg gcgcgcgctt tgcgaaatgg
     3241 cgcgccgtct acaacgtgtc tgacacactt ccgagtcggg ttgcgatcga atccaacgcc
     3301 gaagcgctgg cccgctatgc cgcgatctgc caggaacgag gagtcgtgcc gatcgttgag
     3361 cccgaggtcc tgatcgacgg caatcacagc attgaacgct gtgccgtggt caccgaggct
     3421 gttttgcacg aagtgttcca cgcgctgcat cgtcacaaag tgctgcttga gtacacgctg
     3481 ctgaagccga gcatggtggt gcctggcaag gagcacacgc agcaagtagc tccgcccgaa
     3541 gtggccgcaa acacgatccg catactgagg cgtacggtgc cggctgccgt gcctggaata
     3601 tttttcctgt ctggaggcca gacgcccgca gaagcaaccg cgaatcttga cgcgatcaac
     3661 cggctcggcg cgcagccgtg gcagttgagc ttctcgtacg gccgagcgct gcaggacccc
     3721 gctttgaaag cctggaaagg gcaggctggc aatgtgcacg agacccagaa agcactgctg
     3781 aaacgtgccc ggctcaacgg cgcagctcgc gaagggaagt acttcccttc gctggaggac
     3841 tcactcggct gagtcgaggg ctaccgccac agagcctggg atatattcaa ccggccccag
     3901 tgtcacgcgc acgctatgaa cgccggcatc gtcgagcagc ggaacggccg ctccggcgag
     3961 ttccttgcca tcgaattcca ccctaacgac gccgcgtgca acgccctgcg ggttgtcgat
     4021 cacgaactcg tacgtcgacc gcggcgcacg gtagcgaatc ttgaagctgc gccactgctc
     4081 gggaatgcaa ggatcaatca ggatttcacg accgcgcagc ttgaacccga ggatgaactc
     4141 gatgccagcg cgatacatcc accccgcggc gccggtgtac caactccagc cgccccgtcc
     4201 ggtatgcggt ggttcgctgt acacgtcggc ggcgataacg aagggttcag ccttgtagcg
     4261 atgaatgccg gcgcgcgtcg agctgtggtt gatgggattc aacatctcga acaactccgc
     4321 agcacgctcg ccgtcaccct gcagcgcatg ggcgatcacg caccacaccg cggcctgtgt
     4381 gtactgcgca ccgttttcgc gcactcccgg caggtagccc ttgatgtatc cgggctcctg
     4441 tgccgatcgg tcaaagggcg gtgcgaacag cagcaccatg ctgtcgcccg gcttgaccag
     4501 gtgttcgcca acggattcca ttgctcggcg cgcacgcgcc ggttcacccg cgccggatat
     4561 cagcgcccaa
//
LOCUS       DATIRT010000405         4116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_953707, whole genome shotgun sequence.
ACCESSION   DATIRT010000405 DATIRT010000000
VERSION     DATIRT010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953707"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /gene="thrS"
                     /locus_tag="VLN25_09095"
     CDS             <1..1033
                     /gene="thrS"
                     /locus_tag="VLN25_09095"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HSQ10756.1"
                     /translation="WEKSGHWEKYRENMFTTESENRYYALKPMNCPGHIQIYNANLHS
                     YRELPLRYGEFGACHRNEPSGALHGMMRVRGFTQDDGHIFCTEEQILDECVAYTTLLQ
                     KVYANFGFEHILYKVSTRPAQRIGSDESWDKAEHALVESLKRSGVEFAVSPGDGAFYG
                     PKIEYTLKDAIGREWQCGTMQVDFFMPERLGAEYVAEDNSRKTPVMLHRAIIGSMERF
                     IGMLIENFAGAMPPWLAPVQVAIANISEGQASYAEAVAQTLKKQGFRVDSDLRNEKIT
                     YKIRQLSLEKIPYVLVVGDKEKQSGPEEMRVAVRARGGVDLGVMPLSAFVQRLQDDVG
                     QRRNVGSVA"
     gene            1059..1580
                     /gene="infC"
                     /locus_tag="VLN25_09100"
     CDS             1059..1580
                     /gene="infC"
                     /locus_tag="VLN25_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HSQ10757.1"
                     /translation="MAQERAHRINGEISAPEVRLTGIEGEALGVVNLADAFRMAEEAQ
                     VDLVEIAPTAVPPVCRLMDYGKFKYQEQKKAHETKLKQKQVVVKEVKFRPQTDENDYL
                     TKLRNLTRFLEEGDKAKITLRFRGREMAHQEFGYKLLERVRDDLVALAQVESMPRLEG
                     RQMVMILTPKKKK"
     gene            1700..1897
                     /gene="rpmI"
                     /locus_tag="VLN25_09105"
     CDS             1700..1897
                     /gene="rpmI"
                     /locus_tag="VLN25_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HSQ10758.1"
                     /translation="MPKMKTKKSASKRFEIRAGGSIKRGQAFKRHILTKKTTKAKRHL
                     RGAVLISDADVKSVRRMMPYA"
     gene            1909..2268
                     /gene="rplT"
                     /locus_tag="VLN25_09110"
     CDS             1909..2268
                     /gene="rplT"
                     /locus_tag="VLN25_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HSQ10759.1"
                     /translation="MPRVKRGVTARARHKKVLAQAAGFTRRRNNVFRVAKQAVMKAGQ
                     YAYRDRRTRKRVFRSLWIARINAAVREHGITYSAFINGLGKAEIGIDRKVLADMAVLD
                     KPGFAAVVDKVKASLAA"
     gene            2351..3364
                     /gene="pheS"
                     /locus_tag="VLN25_09115"
     CDS             2351..3364
                     /gene="pheS"
                     /locus_tag="VLN25_09115"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HSQ10760.1"
                     /translation="MDLEQLVNEARAAFAAAMQPAALENEKARFLGKSGAITERLKSL
                     ARLSAEDKREQGARINRAKEAIEALLDERRQSLANAQLNERLAQEAIDVTLPGRLRSA
                     GGIHPVIRTWMRVEEIFASIGFDVADGPEVETDWHNFTALNSPENHPARSMQDTFYVD
                     AQDRFGKPLVLRTHTSPMQVRYARMHAPPIKVIALGRTYRVDSDATHSPMFQQVEGLW
                     IDENVSFADLKAVYTNFLRSFFETDDLAVRFRPSYFPFTEPSAEIDMMFTSGSRKGNW
                     LEISGSGQVHPTVIRNFGLDPEKYIGFAFGSGLERLTMLRYGVDDLRIFFEGDLRFLR
                     QFN"
     gene            3371..>4116
                     /locus_tag="VLN25_09120"
     CDS             3371..>4116
                     /locus_tag="VLN25_09120"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HSQ10761.1"
                     /translation="MDISEKWLRRYADPLIDGARLADELTMAGLEVEDRRPVAPPFSG
                     VVVGKVLTVNPHPNADKLTVCEVDVGQAAPLTIVCGAPNVVAGIRAPCALPGAILPGE
                     FSIKATTMRGVESQGMLCSARELGLSDDHAGLLLLADDAAPGRDVRDVLDLDDQILTI
                     KLTPNRADCFSVVGVAREVAAITGAELTLPAIRPVAPVLDDQLPVSVEAADLCGRFSG
                     RVIRGVDAHEGTPEWMKQRLLRSGQRPISAL"
BASE COUNT          867 a         1195 c         1321 g          733 t
ORIGIN      
        1 gtgggaaaaa tccgggcact gggaaaagta ccgcgagaac atgttcacga cggagtcgga
       61 gaaccggtac tacgcgctga agcccatgaa ctgcccgggc catatccaga tctacaacgc
      121 caatctgcac agctaccgcg agctgccgct gcgctacgga gagttcggcg cctgccatcg
      181 caacgagccg tccggcgcgc tgcacgggat gatgcgtgtg cgcggtttca cgcaggacga
      241 cggccacatt ttctgcacgg aggagcagat cctcgatgaa tgcgttgcct acacgacgct
      301 gctgcagaag gtctacgcga acttcggttt cgagcacatc ctctacaagg tctcgacgcg
      361 accggcgcag cgcatcggct cggacgagag ctgggacaag gccgagcacg cactcgtgga
      421 gagcctgaag cgttcgggtg tggaattcgc cgtttcgcct ggcgacgggg cgttctacgg
      481 cccgaagatc gagtacacgc tgaaagatgc catcggccgc gagtggcagt gcggcacgat
      541 gcaggtcgat tttttcatgc cggagcgcct tggcgccgag tatgtggccg aggacaacag
      601 ccgcaagacg ccggtgatgc tgcaccgggc catcatcggg tcaatggaac ggttcatcgg
      661 aatgctgatc gaaaatttcg ccggcgccat gccgccttgg ctggcgccgg tccaggtggc
      721 gatcgccaat atctccgagg ggcaggccag ttatgccgag gctgttgcgc aaacgctgaa
      781 aaaacaagga tttagagttg acagcgattt gcgcaacgaa aagatcacct ataaaatccg
      841 ccagctcagc ctggagaaaa ttccctatgt cctcgttgtc ggggacaagg agaagcaatc
      901 cgggccagag gaaatgcggg tggccgtgcg tgcccgcggc ggggtggacc tcggtgtgat
      961 gccgttgtcc gcctttgtac aacgtctgca ggacgacgtt ggacagcgcc gcaacgtggg
     1021 cagcgtggct tgatcagaga aaccaggaga agccaaccat cgctcaggaa cgtgcgcacc
     1081 ggatcaacgg tgaaataagc gccccggaag tccgcctaac gggtattgaa ggcgaagctc
     1141 tcggagtcgt gaatctggcc gatgcatttc gcatggccga ggaagcccaa gtcgatctgg
     1201 tggaaattgc tcccaccgcc gtgccacccg tctgcaggtt gatggactac ggcaagttca
     1261 agtatcagga gcagaagaag gcccacgaga ccaagctcaa gcagaagcag gtggtggtca
     1321 aggaagtgaa gttccggccg cagacggacg agaacgacta tctgaccaaa ctgcgcaatc
     1381 tgacgcgctt cctcgaggaa ggtgacaagg ccaagatcac gttgcgcttc cgtggtcgtg
     1441 aaatggccca ccaggagttt ggatacaagc tcctcgagag agtgcgagac gacctggtgg
     1501 cattggcgca ggtcgagtcg atgcctcggc tcgagggacg gcagatggtg atgatcctga
     1561 cgccgaagaa aaagaagtag tcaagacctg ctgatccagc agggttgtgc agcaacagcc
     1621 gcgtagcggc tcaacaagtg gcaacaggct caaagtctcg taagagcgcc cgacgccatc
     1681 aaagaaaggg acggccaaga tgccgaagat gaagacgaaa aagagcgcgt caaagcgctt
     1741 cgagatccgc gcaggcggct cgatcaagcg gggacaggcg ttcaagcgcc acattctcac
     1801 caagaagacc accaaggcga agcgccacct gcgcggcgca gtgctgatca gcgatgccga
     1861 cgtcaaatcc gtgcgtcgca tgatgccgta cgcgtaaggg agactgacat gcctcgagtt
     1921 aaacgtggtg tgaccgcccg cgcgcggcac aaaaaggtcc tggcccaggc tgcggggttc
     1981 acgcgccgtc gcaataacgt attccgcgtc gctaaacagg cggtgatgaa agcggggcag
     2041 tatgcctacc gcgatcgtcg tacgagaaag cgcgtgttcc gttcgctgtg gatcgcgcgg
     2101 atcaatgcag cggtacgtga gcacggcatc acgtacagcg cgttcatcaa tgggctcggc
     2161 aaggccgaaa tcgggattga ccgcaaggtg ctggccgata tggcggtgct cgacaagccg
     2221 ggttttgcgg cggtcgtcga caaggtcaag gcttcgctcg ccgcctaagc gcgcattgcc
     2281 ggtcgcctgc gatcggcaga atcaggaacg gggcctcact gctgggcccc gtttttttag
     2341 gttcatcgaa atggatctcg aacaactcgt aaatgaggcg cgtgccgcgt tcgctgcagc
     2401 gatgcagcct gcggcgctcg agaacgagaa ggcgcgtttt cttggcaagt cgggagctat
     2461 caccgagagg ctgaagtccc ttgcgcgcct gtccgccgag gacaagcggg agcagggtgc
     2521 gcgcatcaat cgggccaagg aggcgatcga agccctgctc gacgaacgcc ggcagtcgtt
     2581 ggcgaatgcg caactcaacg agcgcctggc gcaggaagcc atcgatgtca cgcttcctgg
     2641 acgcctgcgc agtgccgggg gcatccatcc ggtgatccgg acctggatgc gcgtcgaaga
     2701 gatctttgct tccattggtt tcgatgtcgc ggacgggcct gaagtcgaaa ccgactggca
     2761 taacttcact gcgctcaaca gcccggagaa ccatccggcc cgttcgatgc aggatacgtt
     2821 ctacgtcgat gcacaggacc ggttcggcaa gccgctggtg ctgcgaaccc acaccagtcc
     2881 aatgcaggtg cgctatgcgc gcatgcacgc gccaccgatc aaggtcatcg cactcgggcg
     2941 cacgtatcgt gtcgacagcg atgcgaccca ttcgccgatg ttccagcagg tcgaggggct
     3001 ctggattgac gagaacgtga gctttgccga tctcaaagcg gtctacacca acttcctgcg
     3061 aagctttttc gaaaccgatg acctggccgt ccgctttcgc ccgtcctact tcccgttcac
     3121 cgagccgtcg gccgagatcg acatgatgtt caccagcggg tcgcgcaagg gcaactggct
     3181 cgagatctcg ggttcaggcc aggtgcaccc gacggtgatc cgcaatttcg gtctggatcc
     3241 cgaaaagtac atcggtttcg cgttcgggtc cgggctggag cgactgacca tgctgcgcta
     3301 cggagtcgac gatctgcgga ttttcttcga aggtgatctc cgcttcctgc ggcagttcaa
     3361 ctgactcgcg atggatatct cggaaaagtg gctgcgtcgt tacgccgatc cgttgatcga
     3421 cggtgcacgg cttgccgacg aactcaccat ggcgggcctg gaggtcgaag atcgccgccc
     3481 tgtcgcgccg ccgttcagcg gggtggtcgt cggcaaagtg ctcacggtca acccgcatcc
     3541 gaacgctgac aagctgaccg tgtgtgaagt ggatgtcgga caggcagcac cgctcaccat
     3601 cgtttgcggc gcccccaacg ttgtggcggg aatccgggcg ccgtgtgcgc tgccgggtgc
     3661 cattttgccg ggagagttca gcatcaaggc aacgacgatg cggggagtcg agtcacaggg
     3721 gatgctctgc tcggcgcgtg aactcggact gtcggacgac catgcgggct tgctgctgct
     3781 ggcggacgac gccgcaccgg gacgcgacgt gcgcgacgtg cttgaccttg acgaccaaat
     3841 cctcacgatc aagctgacgc cgaatcgcgc cgactgtttc agcgttgtcg gcgttgcgcg
     3901 tgaggttgcc gcgatcaccg gagccgaact gacgttgccc gccatccggc cagtcgcgcc
     3961 cgtcctcgac gatcaactgc ccgtttctgt ggaagccgcc gacctgtgcg gtcgattttc
     4021 cggacgcgtt atccgcggcg tcgatgccca tgaaggcaca ccggagtgga tgaagcagcg
     4081 actgctgcgc agtggccagc gaccgatctc ggcact
//
LOCUS       DATIRT010000406         4255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_560139, whole genome shotgun sequence.
ACCESSION   DATIRT010000406 DATIRT010000000
VERSION     DATIRT010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560139"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            556..903
                     /gene="yajC"
                     /locus_tag="VLN25_09125"
     CDS             556..903
                     /gene="yajC"
                     /locus_tag="VLN25_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HSQ10762.1"
                     /translation="MFFISSAYAQTAPAAQPGAESSLMSIGFMVLIFIVFYFLLIRPQ
                     VKRQKEHKTMVEALNKGDEVVTAGGIVGKINDIADQYITLQVATVNGQPVQLSIQRAA
                     VQTLLPKGTVKSI"
     gene            1049..2923
                     /gene="secD"
                     /locus_tag="VLN25_09130"
     CDS             1049..2923
                     /gene="secD"
                     /locus_tag="VLN25_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HSQ10763.1"
                     /translation="MNRYPLWKYVVIAVALLLGAIYTLPNFFGESPAVQVSSGKPTVK
                     VDAALLTRIETILKDAKFETSGVFFDTAGLNATVRARFRDTDIQLQAKDAIQRALNPN
                     NDDPAYVVALNLISNTPSWLKAVHALPMYLGLDLRGGVHFLLQVDMRAAVDKRLETFS
                     SDIRTLLREKNIRHSGITKSATEIEIRFRDVETRARAEDLIRGQIRELTLREEGTGPD
                     YLLIASLSPAAQKEIQDNALKQNITTLHNRVNELGVAEPTIQQQGADRIVVQLPGVQD
                     VARAKQILGRTATLEIRMVDQDAMASGGVGALTVPERKSDGTVTPVTLKRDVVVTGNQ
                     LNNASATIDSQTQRPAVSVRLDEAGGRAMRAITRDNIGKLMAIVLYEKGKGEAISVAR
                     IQGEFGNQFQITGNFSVQETTDLALLIRSGSLAAPMEIIEERTIGPSLGKENIERGFR
                     STLYGFTAIAIFMIAYYRLFGLISVVALSVNVMLLIALLSLLQATLTLPGIAAIALTL
                     GMAIDANVLINERVREELRNGRNPQQAISEGYDKALATILDSNTTTLIAGVALLIFGS
                     GPVRGFAVVHCLGILTSIFSAVFVSRAIVNLVYGSRKKLAALSVGQIWRPGAADAGAK
                     "
     gene            2946..3881
                     /gene="secF"
                     /locus_tag="VLN25_09135"
     CDS             2946..3881
                     /gene="secF"
                     /locus_tag="VLN25_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HSQ10764.1"
                     /translation="MEFFRIKRTIPFMRYSLILNAISFISFGIAVAFLLTRGLSLSVE
                     FTGGTVMEVSYPQAADLERIRGAIEQKGWGEFQVQNFGTSRDVLIRLPIEKGHTAGEQ
                     ADRVMQTLKAQDPAVELKRVEFVGPAVGEELAHDGALALLLVVAGIMIYLAFRFEWKF
                     SVAAIVANLHDVILVIGMFAIFGWEFSLPVLAAVLAVLGYSVNESVIIFDRVREHFKK
                     YRKMTTVEVIDSAITATISRTIITHGSTLCMTLAMYFFGGPALHYFALALTIGIIFGI
                     YSSVFVAAAIAMYLGVKREDLVKPVKKDEEQELVP"
     gene            3883..>4255
                     /locus_tag="VLN25_09140"
     CDS             3883..>4255
                     /locus_tag="VLN25_09140"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HSQ10765.1"
                     /translation="MSAPVAQRVRVWDLPTRLFHWALAGLVTASWLSGQFGGQPWLEW
                     HFRFGYAILALLVFRLLWGFAGDRYARFASFAPSWHRALGYLRSPQPWAGHSPLGALS
                     VYALLFVAGVQVMTGLFASDGD"
BASE COUNT          719 a         1384 c         1386 g          766 t
ORIGIN      
        1 ccgcgcctgc gaaggcgccc ctaacggggt gccctgtgct gctcggggac gcgggcgggc
       61 ctgcaaactc gcgccctgcg gtcgctcaga caggcaggcc ctcaaaggct cccgcgtccc
      121 ctgcgctgcg cggctccttc ggaggcattc caacgacgtc tccttgggca gcaccgtgcg
      181 gctccggctc aagcacagcg acggggtgaa cccgctcggg gacgccgcag cgttgcgggg
      241 cggcagcggg gcagtgccgg gtgcgtgtct gagcgagcgc agggagctag tttcgcgccc
      301 ggcccgctga cgccctgcag cgcaagggca tctggaggga agcgcaggcc ggtcccgtgc
      361 ggtcgccttt ctttgggact ttctttggcg aagcaaagaa agtcactcgc cgccgggcga
      421 ctcccggctc ggtcgcggca tggcgtgggc gctcaacgcg cagcgccccc gctctgccgc
      481 cctataatcc gccggtttca ccgcaatgcg ccctgcgcgt gcggtccgct tccacatttt
      541 cccgcgggga acccgatgtt tttcatatca agtgcgtatg cccagaccgc gccggcggca
      601 cagccaggcg ccgagtcgtc attgatgtcg atcggcttca tggtgctgat cttcatcgtc
      661 ttctacttcc ttctgatccg cccgcaggtc aagcgccaga aggaacacaa gacgatggtc
      721 gaggcgctga acaagggcga cgaggtcgtc accgccggcg gcatcgtcgg caagatcaac
      781 gacatcgccg accagtacat cacgctgcag gtcgccaccg tcaacggcca gccggtgcag
      841 ttgtcgatcc agcgcgccgc cgtccagacg ctgctgccca agggcacggt gaagtcgatt
      901 tgacgccgct tcgaagcctg gctcccgacg cccttgctgc ggttgtgatc gggcccggcg
      961 cccggccgcc cgaagcccgg tccgctaacc tgattcgacc gagtgcacgt gcgccgcgca
     1021 gtggcgcgta cacgaggtcg accgtcccat gaaccgctac cccctctgga aatacgtcgt
     1081 catcgccgtc gcgctgctgc tcggcgcgat ttacacgctg ccgaacttct tcggcgagtc
     1141 acccgcggtg caggtgtcgt ccggcaagcc cacggtgaag gtcgacgccg cgcttctcac
     1201 ccggatcgaa accatcctga aggacgccaa gttcgagacg agcggcgtgt tcttcgacac
     1261 ggcaggcctt aatgcaacgg tgcgtgcgcg gttccgcgac accgacatcc agctgcaggc
     1321 caaggacgcg attcagcgcg cgctgaatcc gaacaacgac gacccggcgt acgtcgtcgc
     1381 gctgaatctg atctcgaata cgccctcgtg gctgaaagcc gtgcacgcgc tgccgatgta
     1441 cctcggcctc gacctgcgcg gcggcgtgca cttcctgctg caggtcgaca tgcgtgcggc
     1501 ggtcgacaaa cggctcgaaa ccttcagctc ggacatccgc acgctgctgc gcgagaagaa
     1561 catacgtcac tcgggcatta ccaagtcggc caccgagatc gagatcaggt tccgcgacgt
     1621 cgagacccgt gcgcgtgccg aagacctgat ccggggccag atccgcgagc tgacgctgcg
     1681 cgaggaaggc accggccccg actacctgct aatagcctcg ctgtcgccgg cggcgcagaa
     1741 ggagatccag gacaacgcgc tgaagcagaa catcaccacg ctgcacaacc gcgtcaatga
     1801 actcggcgtg gcggagccga ccatccagca gcagggcgcc gaccgcatcg tcgtgcagtt
     1861 gccgggcgtg caggatgtcg cgcgcgccaa gcagatcctc ggccgcacag cgactctcga
     1921 gatccgcatg gtcgatcagg acgcgatggc gtccggtggt gtcggtgcgc tgacggtccc
     1981 ggagcgcaag agcgacggca cggtgacacc cgtcacgctc aagcgcgacg tggtggtgac
     2041 gggcaatcag ctcaacaacg cctcggccac gattgacagc cagacgcaac ggcccgcggt
     2101 gtcggtgcgg ctggacgagg ccggcggccg cgcgatgcgc gcgatcacgc gcgacaacat
     2161 cggcaagctg atggcgatcg tgctttacga gaaaggcaag ggcgaggcga tctcggtcgc
     2221 ccgcatccag ggcgagttcg gcaaccagtt ccagatcacc ggcaactttt cggtgcagga
     2281 gacgaccgac ctcgcgctgc tgatccgctc cgggtcgctt gcggcgccga tggagatcat
     2341 cgaggagcgc acgatcggcc ctagcctcgg caaggaaaac atcgagcgcg gcttccgctc
     2401 gacgctgtac ggcttcacgg ccatcgcgat cttcatgatc gcgtactacc gcttgttcgg
     2461 actgatctcg gtggtagcgt tgagcgtcaa cgtgatgctg ctgatcgctc tgctgtcact
     2521 gctgcaggcc acgctgacgc ttccgggcat tgcggccatc gcactgacgc tcggcatggc
     2581 gatcgacgcc aacgtactga tcaacgaacg cgtgcgcgag gaactgcgca acggccgaaa
     2641 tccgcagcag gccatctccg aaggctacga caaggcactc gcgacgattc tggactcgaa
     2701 cacgacaacg ctgatcgccg gcgtggcgct gctgatcttc ggctcggggc cagtgcgcgg
     2761 ctttgcagtg gtgcactgcc tgggcatttt gacgtcgatc ttctcggcag tgttcgtgtc
     2821 gcgtgcgatc gtcaaccttg tgtacggttc tcgcaagaag ctggccgcgc tttcggtcgg
     2881 ccagatctgg cggccgggcg ctgcagacgc aggcgcgaag taacgggccg caacggagaa
     2941 acacgatgga gttcttccgc atcaagcgca cgattccgtt catgcgctac tcgctgatcc
     3001 tgaatgcgat cagcttcatc tcgttcggca tcgcggtcgc tttcctgctg acacggggac
     3061 ttagcctgtc agtcgagttc accggcggca cggtgatgga ggtgagttac ccacaggccg
     3121 cggatctcga gcgcattcgc ggcgccatcg agcagaaggg atggggcgag ttccaggtgc
     3181 agaacttcgg cacgtcgcgc gacgtgctga tccggttgcc gatcgagaag ggccacaccg
     3241 ccggagagca ggccgatcgc gtgatgcaga cgttgaaggc gcaggacccg gcggtcgaac
     3301 tcaagcgcgt ggagttcgtc ggcccggcgg taggggaaga acttgcgcac gacggcgcac
     3361 tggcgctgct gcttgtagtt gccggcatca tgatttatct cgccttccgc ttcgagtgga
     3421 agttctcggt cgcagcgatc gttgccaacc tgcatgacgt gatcctggtg atcgggatgt
     3481 tcgccatttt cggctgggag ttctcgctcc cggtgctggc ggcggtgctg gcggtgctcg
     3541 gttactcggt caacgagtcc gtcatcatct tcgatcgcgt acgggagcac ttcaagaagt
     3601 accgcaagat gacaacggtc gaggtgatcg acagtgcgat cacggcgacg atctcgcgca
     3661 ccatcattac gcacgggtcg acactgtgca tgacgctggc gatgtacttc ttcggcggac
     3721 cggcgctgca ctacttcgcg ctggcgctga cgatcggcat catcttcggc atctactcgt
     3781 cggtgttcgt cgccgcggcg atcgcgatgt acctcggcgt caagcgcgag gacctggtga
     3841 agcccgtcaa gaaggacgaa gagcaggagc tcgtcccgta gcgtgtccgc gcctgtcgct
     3901 cagcgcgtcc gcgtctggga tctgccgacg cggcttttcc actgggcgct ggcgggactg
     3961 gtgaccgctt cgtggctcag cggccagttc ggcgggcagc cctggctcga gtggcatttc
     4021 cgcttcggct acgccatcct cgcgctgctg gtgtttcgac tgctgtgggg gtttgccggc
     4081 gaccgatatg cacggttcgc gagcttcgcg ccatcatggc accgcgcgct cggttacctg
     4141 cgatcgccgc aaccctgggc cggccatagt ccgctcggcg cgctgtccgt ctatgcgctg
     4201 ctgttcgtcg ccggtgtgca ggtcatgacc ggtcttttcg cctccgacgg tgact
//
LOCUS       DATIRT010000407         3803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_757838, whole genome shotgun sequence.
ACCESSION   DATIRT010000407 DATIRT010000000
VERSION     DATIRT010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757838"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..724)
                     /locus_tag="VLN25_09145"
     CDS             complement(215..724)
                     /locus_tag="VLN25_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HSQ10766.1"
                     /translation="MLPSSAPWAQDTPDDGVAAELVVRALALVDTPYRYGGRTPAGFD
                     CSGFVGYVFAEASGLVLPRRSEDIVRVGDPLERDRLVAGDLVFFNTLGRRYSHVGIYI
                     GDGRFVHAPARGGRVRVERISDPYWRARYNAARRLGPVQPALAAYSVTQSASPTQPTD
                     AAPQERITP"
     gene            948..2129
                     /locus_tag="VLN25_09150"
     CDS             948..2129
                     /locus_tag="VLN25_09150"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSQ10767.1"
                     /translation="MPTDTAKTSTPSAPPKPRVLIADDSRIVRASIVQHIRDRFDIRE
                     VMDGEAAWQAILLDSSIRVVITDLTMPKVDGFELLNRVRSSKVQRIRELPLVVITGED
                     EAAERDRAAGMGATDFIQKGVAAAELIARLEVLVRLSATREALAESQASLESSRTVDS
                     ETELLNVPFFDKQVEKLISFARRNLSDVALICVRIELSLPQQETWEGEVDQRIKLVGR
                     TLAASIRLEDLGTRTDKFEFCVATPSSGLSGVLRFAARLRKVLENVEAAGPGVDVWTC
                     IGLSTLSEELRRSAEELRALAQKRAAQAQQNRSRRIILGTSDGVSPGSMEPKPEDGSM
                     DINLALALIRAGRAAEVVQHLPRLLEQIAPLLKLVRQQKELQATPAEGGIHGEVQGVP
                     R"
     gene            2101..>3803
                     /locus_tag="VLN25_09155"
     CDS             2101..>3803
                     /locus_tag="VLN25_09155"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HSQ10768.1"
                     /translation="MAKYKEFHARSIKDPAGFWAEQAALIDWQTPFSQVLDYSRPPFA
                     KWFVGGTTNLCHNAVDRHVATRGDQPALIYISTETGEEKTYTFRQLHREVNRMASILQ
                     ALGVGRGDRVLIYMPMIPEATFAMLACARIGAIHSVVFGGFAAVSLATRIDDAQPKVI
                     VSADAGSRGGKPVPYKHLLDEAIALAKFPPARVLMVSRGLAEATIVAGRDADYAKLRA
                     EHMNDEVPCVWLESSEPSYILYTSGTTGKPKGVQRDTGGYAVALAASMKLIFCGNPGE
                     TYFATSDIGWVVGHSYIIYGPLLAGMATIMYEGTPIRPDGGVWWSIVEKYKVTTMFSA
                     PTAIRVLKKQDPAFLKKYDLSSLRLLFLAGEPLDEPTARWIADGIGKPIIDNFWQTET
                     GWPILAIPRGVEALPSKFGSPGVPCYGYSVKIYNEATGEEITAHHEKGVVAIDGPLPP
                     GCMTTVWGDDARFVKTYWTTIPDRQVYSTFDWGIRDEDGYFFILGRTDDVINVAGHRL
                     GTREIEESLSSHPNIAEVAVVGVADQLKGQVAMAFAILKDPSLADTPEKKLAHEGEMM
                     KT"
BASE COUNT          741 a         1214 c         1211 g          637 t
ORIGIN      
        1 gatgccacca agcacggtca gttgaagacc gcgacgcagc cagatttcgg cgttccagag
       61 tgagcgggtc gttctcattg agggttccgt gtcagggtca ttcgcgtcgg acgttccaac
      121 gcgcagatcg aacggtaacg aaaactcacc tcaaatgcaa atagttctca tttgcatcca
      181 ctgatccatg tcaacgagag ggacttggca cccgtcaggg ggtaatgcgc tcttgcggcg
      241 ccgcgtcggt gggctgcgtg ggagacgcgg actgcgtcac ggagtacgcc gccagcgccg
      301 gctggaccgg accaaggcgc cgggccgcgt tgtaacgggc gcgccagtaa ggatcggaga
      361 ttcgctcgac tcgcacccgg cctccgcgcg ccggcgcgtg aacgaagcgc ccgtcaccga
      421 tgtagatgcc gacatgcgag tagcgacgcc cgagagtatt gaaaaacacc agatcgccgg
      481 cgaccagacg gtcgcgctcg agggggtcgc cgacccggac gatgtcctcg gatcgacgtg
      541 ggaggacgag gccagacgct tccgcgaaca catagccgac aaaaccgctg cagtcgaatc
      601 cggccggtgt gcggccgcca taccgatagg gggtgtcgac gagagcgagc gcgcgtacga
      661 ccagttccgc ggccacgccg tcgtcaggcg tgtcctgcgc ccagggcgcc gaacttggca
      721 gcatggaaac tgccacacca aacgcaatcc agacccgttt caaccgtccg cttcgtcgca
      781 tcagcttggc gcgcggctgc cacaatgggc gcgaacatag cgcattccag cgagaaaacc
      841 taaaggtgac cggggcttac gccgatcatt tacgttaaat tccatccgca attcacacgg
      901 atggccggcg tcggagcgcc ggatcgcagt tccgtcacga ggttggcgtg cccaccgaca
      961 ccgctaagac atcaacgccg agcgcgcctc ccaagccgcg cgtcctgatt gcagacgatt
     1021 cccgcatcgt gcgggcgtcc atcgttcagc acatccgcga ccgcttcgac atccgcgagg
     1081 tcatggacgg cgaggcagcc tggcaggcaa tcctgcttga ctcctcgatc cgggtcgtca
     1141 tcaccgacct gacgatgccg aaggtggacg gcttcgagtt gctcaaccgg gtccgcagct
     1201 cgaaagttca gcgcattcgc gagctgccgc tcgttgtgat taccggcgag gacgaagcgg
     1261 cagagcgcga tcgcgccgcc ggaatgggcg caacggactt tatccagaaa ggcgtggccg
     1321 ccgccgagct gatcgcccgg ctggaagtgc tggtgcggct ttcggccacc cgggaagcgc
     1381 tggccgaaag tcaggcatcg ctcgaatcgt cgcgcacggt cgacagcgag accgagctgc
     1441 tcaacgtgcc tttcttcgac aagcaggtcg aaaagctcat ttcgtttgcg cgacgcaatc
     1501 tgtcggatgt ggcactgatt tgcgtgcgaa tcgagctgtc cttgccgcaa caggagacct
     1561 gggagggcga agtcgatcag cgcatcaagc tggtgggccg gacgctggcc gcctcgatcc
     1621 ggctggaaga cctcggtact cggaccgaca agttcgagtt ctgcgtcgcc acgccctctt
     1681 ccggcctatc gggtgtgctg cgattcgccg cacggctgcg caaggtgctc gaaaatgtcg
     1741 aggcggccgg acccggcgtg gatgtctgga cctgcatcgg cctgtcgacg ctttctgaag
     1801 aattgcggcg cagcgcagaa gaacttcgtg cgctggctca aaagcgcgcg gcacaggcgc
     1861 agcaaaatcg ctcgcgccgc atcatcctcg ggacgtcgga cggagtctcg cccggtagca
     1921 tggaaccgaa gcccgaggac ggttcgatgg acatcaatct cgcgctcgcg ctgatccgcg
     1981 ctggccgggc tgccgaagtg gtgcaacatc tgcccagatt gctggagcag atcgcgccac
     2041 tcctgaaact ggtgcggcaa cagaaagaac tacaagccac accagcggag ggaggcattc
     2101 atggcgaagt acaaggagtt ccacgctagg tcgatcaagg atccggccgg cttctgggcc
     2161 gagcaggccg cactgatcga ctggcagacc ccgttctcgc aggtcctcga ttattcccgg
     2221 ccgccctttg cgaagtggtt cgtcggcggg accaccaatt tgtgccacaa cgcggtggat
     2281 cgtcacgtgg cgacgcgcgg cgaccagccg gcactgatct acatctccac cgaaaccggc
     2341 gaagaaaaga cctacacctt ccggcaattg caccgggaag tgaaccgcat ggccagcatc
     2401 ctgcaggcgc tcggtgtcgg ccgcggggat cgcgtgctga tctatatgcc gatgattccg
     2461 gaagccacgt ttgcgatgct ggcatgcgcc cgcatcggcg cgattcactc ggtggtgttc
     2521 ggcggctttg cggccgtcag cctcgccaca cgtatcgacg atgcgcagcc aaaggtcatt
     2581 gtctcggccg acgccggttc gcgcggcggc aagccggtgc cgtacaagca cctgctcgac
     2641 gaggcgattg cactcgccaa gttcccgccg gcccgtgtgc tgatggtcag tcggggcctg
     2701 gccgaggcca cgatcgtggc cggccgcgac gccgactacg cgaagctgcg cgccgagcac
     2761 atgaacgacg aagtgccgtg cgtctggctc gaatcgagcg aaccgagcta catcctctat
     2821 acgtccggta cgaccggcaa gcccaagggc gtgcagcgcg acaccggcgg ctacgcggtg
     2881 gcgctcgcgg cgtcgatgaa gctcatattc tgcggcaatc ccggggagac ctacttcgcc
     2941 accagcgaca tcggctgggt ggtgggccac agctacatca tctacggtcc gctgctggcc
     3001 gggatggcca ccatcatgta cgaaggcacg ccgatccgtc ccgacggcgg cgtttggtgg
     3061 agcatcgtcg agaagtacaa agtcacgacg atgttctctg cgccgaccgc gatccgcgtg
     3121 ctgaagaagc aggacccggc gttcctcaag aaatacgacc tgtcttcgct tcgactgctg
     3181 ttcctcgcgg gcgagccgct cgacgagcca accgcgcgct ggatcgccga cggcatcggc
     3241 aagcccatca tcgacaattt ctggcaaacc gaaacgggct ggccgatcct ggcaattcct
     3301 cgcggcgtcg aggcgctgcc gtcgaagttc ggctcgccgg gcgtgccctg ctatggctac
     3361 tccgtcaaga tctacaacga agccaccggc gaagaaatca ccgcccacca cgaaaagggc
     3421 gtggtcgcga tcgacggccc gctgccgccg ggatgcatga ccacggtctg gggtgacgac
     3481 gcgcgctttg tgaaaacgta ctggaccacc attcccgatc ggcaggtcta ttcgaccttc
     3541 gactggggca tccgcgacga ggacggttac ttcttcattc tcggccgcac cgatgacgtg
     3601 atcaacgtcg ccggccaccg cctcggcacg cgcgagatcg aggagtcgct gtcatcacat
     3661 ccgaacatcg ccgaggtcgc cgtcgtcggt gttgccgatc agctgaaggg gcaggtcgcg
     3721 atggctttcg cgatcctgaa ggacccgagc ctggccgaca cgccggagaa gaagctcgcg
     3781 cacgaaggcg agatgatgaa gac
//
LOCUS       DATIRT010000408         7826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1544680, whole genome shotgun sequence.
ACCESSION   DATIRT010000408 DATIRT010000000
VERSION     DATIRT010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7826
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1544680"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..1357
                     /gene="gshA"
                     /locus_tag="VLN25_09160"
     CDS             26..1357
                     /gene="gshA"
                     /locus_tag="VLN25_09160"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HSQ10769.1"
                     /translation="MSNTLPQLATANTGPLLALEQKIVDEMPAIERWFRLQWQDHTPP
                     FYGSVDLRNAGYKLAPVDMNLFPGGFNNLSEEMLPCAVQAAQAAIEHLCPDARHLVLV
                     PERHTRNLFYLANVARLQRILRQTGLEVRIGTLSDEVKEPTRIELPSGDVLTVEPLVR
                     QGDKIGVAGFTPCAVVLNNDLSAGIPPILRGLEDQVLVPPLQAGWAIRRKSNHFAAYD
                     VVAAEFAQLIDIDPWLINPAFGRCGEVNFHERAGEECLASNIDFVLGKMREKYREHGI
                     EDTPYVVVKADAGTYGMGVMTVKDASEVRGLNRKQRNKMSVVKEGLEVSEVIIQEGVP
                     TNELVDNAVAEPVVYMIDRYVVGGFYRVHTERGRDENLNAPGMHFVPLAFAGSCNLPD
                     CNSAAGESASNRFYAYGVIARLALLAASVELERTAHRVEQASAPIAAAVAG"
     gene            1378..2334
                     /gene="gshB"
                     /locus_tag="VLN25_09165"
     CDS             1378..2334
                     /gene="gshB"
                     /locus_tag="VLN25_09165"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HSQ10770.1"
                     /translation="MALALLFIVDPLDQLKAYKDTSVAMMREAGRRGHAVYACTPDEL
                     AATEAAVTARVHRLHLTNDDHAWYEEQGAQGGEERDLASFDAVLMRKDPPFDVEFLNA
                     TLLLSRAAALGARVVNDARALRDHNEKLAVLEFPQFTVPTLVTREAERIRGFWAYHGD
                     IIVKPLDGMGGTGVFRLQPGDPNAGSILETVTARGARTAMAQKFIPAIRDGDKRVLVI
                     DGEPVPYCLARIPKAGETRGNLAAGGIGRAQPLGARDREIARVVGQALLPRGLRLLGL
                     DVIGEFLTEINVTSPTCFVEIAEQAGCNVAGIFIDALERGSS"
     gene            2426..2890
                     /locus_tag="VLN25_09170"
     CDS             2426..2890
                     /locus_tag="VLN25_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HSQ10771.1"
                     /translation="MTGIVIIAHAPLASALEHCVEHVYTCEPDMAKANLRSLDVEPGA
                     AIEVLAERAARLVAEVNTGSGVLVLTDAFGATPGNLAAGLNDPGRVTVVAGVNLPMLL
                     RAVCYRGGTLAEVAEKALVGGQQGMVQVATTPVQNQGYNPQGNDQQRLHHQQ"
     gene            2859..3128
                     /locus_tag="VLN25_09175"
     CDS             2859..3128
                     /locus_tag="VLN25_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HSQ10772.1"
                     /translation="MISRDFTISNKLGLHARPSAQITQVASRYFAEVWISKSERRVNA
                     KSIMGVMMLAAGQGSVLTVEADGPDEAQAIDAIGELIASGFGELD"
     gene            3151..4968
                     /gene="ptsP"
                     /locus_tag="VLN25_09180"
     CDS             3151..4968
                     /gene="ptsP"
                     /locus_tag="VLN25_09180"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HSQ10773.1"
                     /translation="MQAGTEPRRDTLTGSGVTRGIAIGRAHLLAPSELDVRQVTIERR
                     DVVHEIARMTRAFAVVRDELDGLKSGISPDAPTEVRAFLDLHRLILEDSLLSEAPRDL
                     IRTRLINAEWALTIQLEEVCKQFEAIDDEYLRERSTDVRQVVERVLNSLAEGTPTRVT
                     FPKKIAPGERLVLVARDLAPADVLHLKDRADLDLAGFVTELGGPTSHTAILARSLGLP
                     AIVGADHARERIVEGDVVVIDADADLVVVNPSEEELQEFRRRSRADAEHRTQLRKLRG
                     KLARTRDGIAIDLLANIELPEDARDALEAGADGIGLFRSEFLFMDRDELPTEDEQYEA
                     YAKVARVMKGKPVVIRTLDIGADKVLTETARESLGMGRGAPRPESNPALGLRAIRYCL
                     AYPELFQTQLRAILRASEVGTVRILIPMLAHVHEIEQAAAFVARAKEQLKERRLKYDA
                     RIQLGGMVEVPAAALSLPEFVQRLKFLSIGTNDLIQYTLAIDRTDSTVAHLYDQMHPA
                     VLRLIAQTIRTGTKSRVPVSVCGEMAGELEMTELLLGMGLRQFSMHPSQILPVKERLF
                     ELAFKDSGRFAGRVLRMSDPVKIRHLLQREVRTVAHFVQ"
     gene            complement(5038..5592)
                     /locus_tag="VLN25_09185"
     CDS             complement(5038..5592)
                     /locus_tag="VLN25_09185"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HSQ10774.1"
                     /translation="MKLMKWLATSVALVAFGLSSATAADKAKQDEVLKSAQSTLADFY
                     KADPKLKGQVEKAPGYATFTTFGISFLVGGAGGKGVVHDKATKKNTFMELAQASAGLQ
                     IGASDMRYLFIFDTPASMQQFIDKGWDASASASAGAGVEGKGSASASSGTFTGGKFYQ
                     LTKNGLQAGVAASGTKVWKDKDLN"
     gene            5884..7026
                     /locus_tag="VLN25_09190"
     CDS             5884..7026
                     /locus_tag="VLN25_09190"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HSQ10775.1"
                     /translation="MNAKPRSVGVVTPQVLRFDAPLALTSGASIAGYEVMIETYGRLN
                     ADRSNAVLVCHALNASHHVAGTHGEDPGSVGWWDNMVGPGKPVDTERFFVVGVNNLGS
                     CFGSTGPMSTDPSTGSPYGARFPVVTVEDWVHAQARVADVLGIERWAAVMGGSLGGMQ
                     ALSWAIQYPQRIRHCISIAAAPKLSAQNIAFNEVARTAIRSDPDFHGGNYYAHGVVPR
                     SGLRVARMIGHITYLSGADMTEKFGRSMQTDDYQFHYDVEFEVESYLRYQGDKFSEYF
                     DANTYLLITRALDYFDPARHTGGNLAQALAPASARFLVVSFTTDWRFPPERSREIVEA
                     LVDKERDVAYAEIDAPHGHDAFLLEDPRYHRVVRAYFDNIATEIAP"
     gene            7187..7771
                     /gene="metW"
                     /locus_tag="VLN25_09195"
     CDS             7187..7771
                     /gene="metW"
                     /locus_tag="VLN25_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HSQ10776.1"
                     /translation="MTLRVDLAQIAEWVAPGSRVLDLGCGDGSLLAHLRDARSCRGYG
                     VEIDDDHVARCVSQAINVVQQNLDAGLRMFGDNMFDTVVLSQTLQAMHNAEGILREMA
                     RVARQGIVSFPNFGHWRHIVSLLAGRMPVSRTIPYQWYDTPNIHLCTLKDFEILAAKV
                     GLVITGRTVLAEGRPVNVAPALRATVAIYRFEAR"
BASE COUNT         1345 a         2544 c         2576 g         1361 t
ORIGIN      
        1 tggaccgcgc agaagcgagc ccgtcgtgtc gaacacactt ccccagctgg ccacggccaa
       61 cacgggcccc ctgttggcgc tcgagcagaa gatcgtcgac gagatgccgg cgatcgagcg
      121 ctggttccgg ctgcagtggc aggaccacac cccgccgttc tacggctcgg tcgacctgcg
      181 caacgccggc tacaagctgg cgccggtcga catgaacctg tttccgggcg gcttcaacaa
      241 cctttcggaa gaaatgctgc cgtgcgcggt gcaggctgcc caggcggcga tcgagcacct
      301 gtgcccggat gcccgccacc tggtgctggt gccggagcgt cacacgcgca acctctttta
      361 cctcgccaac gtcgcgcgac tgcagcgcat cctgcgccag accggactgg aggtgcgcat
      421 cggcacgctc tccgatgagg tcaaggagcc aactcgcatc gagctgccga gcggtgatgt
      481 gctgaccgtc gagccgctgg tgcgccaggg tgacaagatc ggcgtcgcgg gcttcacgcc
      541 ctgtgcggtc gttctcaaca acgatctgtc ggcaggcatt ccgccgatcc tgcggggtct
      601 cgaggatcag gtcctggtgc cgccactgca ggccggctgg gcgatacggc gcaagtcgaa
      661 tcacttcgcc gcctacgacg tggtcgcggc cgagttcgcg caattgatcg acattgaccc
      721 gtggctgatc aacccggcct tcgggcgctg cggcgaggtc aacttccacg agcgcgctgg
      781 cgaggagtgc ctcgccagca atatcgactt cgtgctcggc aaaatgcgcg agaagtatcg
      841 cgagcacggc atcgaggaca cgccgtacgt ggtcgtcaag gccgacgccg gcacctatgg
      901 catgggcgtg atgacggtca aggatgcttc cgaggtgcgg gggctgaacc gcaagcagcg
      961 caacaagatg tcggtggtca aggagggact cgaggtcagc gaggtcatca tccaggaagg
     1021 cgtgcccacc aatgaactgg tcgacaacgc ggtcgccgag ccggtggtct acatgatcga
     1081 tcgctatgtc gtcggtggct tttaccgcgt gcacacggag cgcgggcgcg acgaaaacct
     1141 gaacgcaccg ggcatgcact tcgttccgct cgcgtttgcc ggcagctgca acctgcccga
     1201 ctgcaacagc gcggccgggg aaagcgcatc caaccgcttc tatgcctacg gggtgattgc
     1261 gcggctggcg ctgctcgccg cctcggtcga actcgaacgc actgcgcacc gcgttgaaca
     1321 agcctctgcg cccatcgcgg cggcggtcgc cggctgatcg ccccccgatc ggaccgcatg
     1381 gcgctcgcgc tgctgttcat cgtcgacccg ctcgaccagc tcaaggccta caaggacacg
     1441 agcgtcgcga tgatgcgcga ggccgggcgg cgcggccacg cggtgtatgc gtgcacaccg
     1501 gacgaactcg ctgccacgga ggcggcagtg acggcgcgcg tccatcgcct gcaccttacg
     1561 aatgacgatc acgcctggta cgaggagcag ggtgcgcagg gcggcgaaga gcgcgacctg
     1621 gcgtccttcg atgcggtgct gatgcgcaag gacccgccgt tcgatgtcga gttcctcaac
     1681 gcaacgctgc tgctgtcgcg cgcggcggca ctgggagcgc gggtggtcaa cgacgcgcgg
     1741 gcactgcgcg accacaacga gaagcttgcc gtcctcgaat tcccgcagtt caccgtgcca
     1801 accctggtaa cccgggaggc ggagcgtatc cgggggttct gggcctatca cggcgacatc
     1861 atcgtcaagc cgctggatgg catgggcggc accggtgtgt tccgcctgca gccgggcgac
     1921 ccgaatgcgg gctcgatcct tgaaacggtc accgcgcgcg gcgcgcgcac ggcgatggct
     1981 cagaagttca ttcctgcgat tcgcgacggc gacaagcgtg tgctggtcat cgacggcgaa
     2041 ccggtgccgt actgcctggc gcgtatcccg aaagcgggcg aaacgcgcgg caacctggcg
     2101 gcgggcggca tcggacgcgc gcagcccctc ggcgcacgcg accgcgagat cgcccgggtc
     2161 gttggccagg cactgctgcc gcgcggcctc cgcctgctcg ggctggacgt tatcggcgag
     2221 ttcctcaccg agatcaacgt caccagcccg acctgcttcg tcgagatcgc cgagcaggca
     2281 ggctgcaacg tcgcgggaat cttcatagat gccctggaaa ggggatcgtc ataatgaatg
     2341 cctgcttttg ttctgcgcgc gccactttaa acgtgcgagg tgcacgtgcg acgtgcagcg
     2401 tcgcgtacac cgagtgcgat tctttatgac cggcatcgtc atcattgccc atgccccgct
     2461 ggccagtgcg ttggagcact gtgtcgagca tgtctacacc tgcgagcccg acatggccaa
     2521 ggccaatctc cgctcgctcg atgtcgagcc cggcgccgcg atcgaagtcc tcgccgagcg
     2581 cgcggcgcga ctggttgccg aggtgaatac cggttccggt gtgctggtgc tgaccgatgc
     2641 gttcggcgcg actccgggca acctggcggc agggctgaac gacccggggc gcgtcacggt
     2701 ggtcgccggc gtcaatctgc cgatgctgct gcgcgcggtc tgctaccgcg gcggcacgct
     2761 cgccgaggtt gccgagaagg cgctcgtggg aggacaacag ggaatggtgc aggtcgcgac
     2821 gacgccggtg cagaaccagg ggtacaaccc gcagggcaat gatcagcaga gacttcacca
     2881 tcagcaataa gctcggtctg cacgcgcgtc cctcggctca aatcacccag gtggccagtc
     2941 gctacttcgc cgaagtctgg atctcgaaga gcgagcgccg ggtgaacgcg aagtcgatca
     3001 tgggcgtgat gatgctggcc gccgggcagg gcagcgtgct cacggtcgag gccgacgggc
     3061 cggacgaggc gcaggcgatc gatgccatcg gtgagctgat tgcctccggc tttggcgaac
     3121 tggattgatc gaggcgaatc aggcaggcag atgcaggcag gcacggaacc cagacgcgac
     3181 acgctgaccg gcagcggggt gacgcgcggc atcgccatcg gccgcgcgca cctgctggca
     3241 ccgtccgaac tcgacgtgcg ccaggtcacg atcgagcggc gtgacgttgt gcacgagatt
     3301 gcgcgcatga cgagggcctt tgcggtcgtc cgcgatgaac tcgatggcct caagagcggg
     3361 atctctccgg atgcacccac cgaggtccgc gccttcctcg acctgcaccg gctgatactc
     3421 gaagactccc tgctgtcgga ggcgccgcgc gacctgatcc gcacgcgcct gatcaatgcc
     3481 gaatgggcgc tgacgataca gctcgaggaa gtgtgcaagc agttcgaggc gatcgacgac
     3541 gaatatctac gcgaacgttc gaccgacgtg aggcaggtgg tcgagcgtgt gctgaattcg
     3601 ctcgcagaag gcacgccgac gcgcgtgact ttcccgaaga agattgctcc cggcgaacgg
     3661 ctggtactgg tcgcgcgcga cctggctccg gccgatgtgc tgcacctgaa ggatcgcgcg
     3721 gacctcgacc tggcgggctt cgtgaccgag ctcggggggc cgacctcgca tacggcgatt
     3781 cttgcgcgca gcctggggtt gccggcgatc gtcggcgccg atcacgcgcg tgagcgcatc
     3841 gtcgagggcg atgtggtcgt cattgacgcg gatgccgacc tcgttgttgt caatccgtcc
     3901 gaggaagagc tgcaggagtt tcgccggcgc tcgcgcgccg atgccgaaca ccggacgcag
     3961 ctgcgcaagc tcaggggcaa gctggcgcgc acgcgtgacg gcattgcgat cgatctgctg
     4021 gccaacatcg agctgcccga agacgcgcgc gacgcgctcg aggccggtgc cgacggcatc
     4081 ggtctgttcc gttccgagtt cctgttcatg gaccgtgacg aactgcccac cgaggacgag
     4141 cagtacgaag cctatgcgaa ggtcgcgcgc gtgatgaagg gcaagccggt cgtcatccgc
     4201 acgctcgata tcggcgccga caaggtcctc accgagacgg cgcgcgaaag cctgggcatg
     4261 ggccgcggcg cgccgcggcc ggaatcgaat ccggcgctcg gattgcgcgc gatccgctac
     4321 tgcctggcct acccggaact cttccagacg cagttacggg caatcctgcg ggcttccgaa
     4381 gtcggcacgg tgcgcatttt gataccgatg ctcgcccacg tgcacgagat cgaacaggcc
     4441 gccgccttcg tcgcacgtgc gaaggagcag ctaaaggaac gtaggctcaa gtacgacgcg
     4501 cgcatccagc tcggaggcat ggtcgaggtg ccggcggcgg cgttgtcact gcccgagttc
     4561 gtgcagcggc tcaagttcct gtcgatcggt accaacgacc taatccagta cacgctggcg
     4621 atcgaccgca ccgacagcac ggtggcccat ctttacgacc agatgcaccc ggccgtgctg
     4681 cgactgatcg cgcagacgat ccgcaccggg accaagtccc gcgttcccgt ctcggtctgc
     4741 ggcgaaatgg ccggggaact cgagatgacc gaactgctcc tcggtatggg gctgcggcag
     4801 ttctcgatgc atccgtcgca gatcctgccg gtcaaggagc gactgttcga actggcattc
     4861 aaggactctg gacgttttgc cggtcgcgta ctgcgcatgt cggacccggt gaagatccgt
     4921 catctgttgc agcgggaagt ccggaccgtg gcgcactttg tgcaatagcc cggggtacca
     4981 aagaaaaagg cccgggttcg ccgggccttt tcataggcgc ttcgaacgcg cgcaggatta
     5041 gttcaggtct ttatccttcc acaccttggt gccgctggca gccacgcccg cctgcaatcc
     5101 gttcttggtc agttgataga acttgcctcc ggtgaaggta ccgctgctag cgctcgccga
     5161 tcccttcccc tcgacgccgg caccggcgga cgcggaggcg gaggcatccc agcctttgtc
     5221 gatgaactgc tgcatgctgg ccggggtgtc gaagatgaac aggtagcgca tgtccgaggc
     5281 tccgatctgc agaccggcgc tggcctgtgc cagttccatg aacgtgttct tcttcgtcgc
     5341 cttgtcgtgt accacgccct tgccgccggc gccgcccacc aggaagctga tgccaaatgt
     5401 ggtgaacgtg gcatagccgg gagctttctc gacctggccc ttcagtttcg gatccgcctt
     5461 gtagaagtcg gccagcgttg actgcgccga cttcaggact tcgtcctgct tcgctttgtc
     5521 cgcagccgtc gcggacgaca gcccgaatgc caccaatgcc acgctcgtgg ccagccattt
     5581 catcagtttc atgtggtttt cctttccagt gtcggactca atttctgcac cgactcactc
     5641 acgtcggcca agggtccatg cgcgaagtct acgctcgccc gcaagcgctc tccttgacgt
     5701 ttgacacgtc gcggcacagt cactagcctt cgcgatctgc gccgatttgc cgactcgctt
     5761 gcgggcgcgt gaggaaaatg cccctccgaa aaccgctaaa gaggtcaagc agccctgctg
     5821 ccccgcaccc gagccggcgt cgtgcccctc gggtttttgt tttctgactg tccggaactg
     5881 ccgttgaacg caaagcctcg ctcggttggt gtcgtcacgc cgcaggtcct gcgcttcgat
     5941 gcgccgcttg cgctgaccag tggcgcctcg atcgccggct acgaagtgat gatcgagacc
     6001 tacggccgcc tgaatgcaga ccgaagcaat gcggtgctgg tctgccatgc actgaatgct
     6061 tcgcatcacg tggccggcac gcatggcgag gacccgggca gtgtcgggtg gtgggacaac
     6121 atggtgggtc cgggcaagcc cgtggacacg gagcggttct tcgtcgtcgg cgtcaacaac
     6181 ctcggttcgt gctttggatc gaccggtccg atgtcgaccg atccgtcgac cggttcgccc
     6241 tacggagcgc gctttccggt cgtgacggtc gaggactggg tgcatgcgca ggcgcgcgtg
     6301 gcggacgtgc tcggcatcga gcgctgggca gcggtgatgg gtggatcgct cggcggtatg
     6361 caggcgctgt catgggcgat ccagtatccg cagcgcatcc ggcactgcat ctcgatcgcc
     6421 gcggcgccca agctgtcggc gcagaacatc gccttcaacg aagtagcgcg caccgcgatc
     6481 cgctccgatc cggactttca cggcggcaat tactacgcgc acggcgtggt gccccggagc
     6541 ggtctgcgcg ttgcacgcat gatcggccac atcacctatc tgtcgggcgc cgacatgacc
     6601 gagaagttcg gccggtcgat gcagaccgat gactaccagt tccactacga cgtcgagttc
     6661 gaagtcgaaa gctaccttcg ctatcagggc gacaagttct ctgagtactt tgatgcgaac
     6721 acctatctgc tgatcacgcg cgctctcgac tactttgacc cggcccggca caccggcggc
     6781 aacctggcgc aggcgctcgc tccggccagc gcgcggttcc tcgtcgtgag cttcaccacc
     6841 gactggcgct tcccgccgga gcgatcgcgc gaaatcgtcg aggcgctggt cgacaaggaa
     6901 cgcgacgtcg cctatgccga aatcgatgcg ccccacggcc acgacgcgtt cctgctcgag
     6961 gatccccgct atcaccgcgt cgttcgggcc tacttcgaca acatcgccac ggagatcgcg
     7021 ccatgagcgc cgcacggccg ctcgaagagc gctcgcgtcc cccgcggggg gacgcaggcc
     7081 aatgcagtgg catgccctgc ggcatgccac ccggccgacg ccgcttgcgt atgccgacgc
     7141 aagcgggcat ggcgcgtagc gccctgccga agggcgaggg ggcaacatga cgctgcgcgt
     7201 cgacctggct cagatcgccg agtgggtagc acccggcagc cgcgtactcg acctcggctg
     7261 cggcgacggc tcgctgctgg cgcacctgcg cgatgcgcgt tcgtgccgcg gctatggcgt
     7321 ggagatcgac gacgaccacg tggcgcgctg cgtcagtcag gccatcaatg tggtgcagca
     7381 gaacctcgac gccggcctgc gcatgtttgg cgacaacatg ttcgacacgg tcgtgctgtc
     7441 gcagaccctg caggcgatgc acaacgccga gggcatcctg cgcgagatgg cgcgcgtcgc
     7501 gcgtcagggc atcgtcagct ttccgaactt cggccactgg cgccacatcg tttcgctgct
     7561 ggccggtcgc atgccggtgt cacgcacgat tccgtatcag tggtacgaca cgccgaacat
     7621 tcacctgtgc acgctgaaag acttcgaaat cctggcggcc aaggtgggac tcgtgatcac
     7681 gggacgcacg gtgttggccg agggccggcc ggtgaacgtc gcgccggcgc tgcgggcgac
     7741 cgtcgcgatc tatcgcttcg aggcgcgctg agcgccttcg taagagaaac ctatatggcc
     7801 gattcgctgc gcgacaagct gattcg
//
LOCUS       DATIRT010000409         3266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1545553, whole genome shotgun sequence.
ACCESSION   DATIRT010000409 DATIRT010000000
VERSION     DATIRT010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1545553"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..1175
                     /locus_tag="VLN25_09200"
     CDS             9..1175
                     /locus_tag="VLN25_09200"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HSQ10777.1"
                     /translation="MMRTITLLGATGSIGSSALDVIARHPDRYRVHALTAQSSVDALA
                     DLALRFRPALVVIGDATLEPVLRAALRERGATAEPRAGAAALAEVAQAPEVDTVLASI
                     VGSAGLVPTLAAAHAGKRLLLANKEAIVCAGGLLMAALRAAGGELLPVDSEHNAIHQC
                     LAGTGEGQRGRARLVLTASGGPFLRRRDLDAVTPDEACAHPNWVMGRKISVDSATLMN
                     KGLEVIEASWLFDMPPERIDVVIHPQSVIYSMVEFPDGSVLAQLGSPDMRTPLAYALA
                     WPDRVESGAKRLDFARLGSLTFEAPDRERFPCLGYAYAALRSGGAASAVLNAANEYAV
                     EAFLQRRLPFTQIAPTIERVLDEFQPTAPASVEEVLEIDRQARDRAALAVALHA"
     gene            1185..2534
                     /gene="rseP"
                     /locus_tag="VLN25_09205"
     CDS             1185..2534
                     /gene="rseP"
                     /locus_tag="VLN25_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HSQ10778.1"
                     /translation="MNLITTIAAFLVTLGILIVIHELGHYWVARWCGVKVLRFSIGFG
                     RPIARWVRGPDRTEWVVSALPLGGYVRMLDERDPDTPVTDEDLPRAFNRQPVGKRFAI
                     VLAGPVANLLLAIFVYWLLNVIGVFEPRATLGAPAAATAAATAGVEAGDTVTAVDGDG
                     VRSWNELRWLLLQRAVARAPIEIEVEGVDGQRRTLILEAGPLTAADLESDVVNRLGFI
                     PYSGPPRVNRLTEDGAAARGGLRPGDTIIEANGAKVGSARDLIERIRSSAGKSMDLGV
                     QREGEVVRLTVTPALVNDEQGKSIGRIGAELNDRPPLVKVQYGPFESIGLATGKTWDT
                     AIFSLKMLGKMITGEASWKNLSGPVTIADYAGQTARIGLAPFLNFLALVSISLAVLNL
                     LPVPMLDGGHLLYYLIEILKGSPPADWVIEWGQRAGVALLGLLTMLALYNDLMRLLS"
     gene            2571..>3266
                     /locus_tag="VLN25_09210"
     CDS             2571..>3266
                     /locus_tag="VLN25_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008543581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HSQ10779.1"
                     /translation="MSSGRSVPVALRRVAAAVIAAYCFSAQAQEFVVRDIRVEGVQRT
                     EAGTVFTYLPIRVGDRFDPERGTQAIRALYASGLFKDVRLEVDGDVLVVIVDERPAIA
                     TIDLAGVKEFEKDQVLKSLREVGLADGRIYDRALLERAEQELRRQYLSRGLYGVEIKT
                     TVTPIERNRVNISVAVEEGDTSKIRTIAFTGNKVFSDGTLRSQMDLSTPNWISWYTKR
                     DQYSRQKLGADLES"
BASE COUNT          541 a         1033 c         1092 g          600 t
ORIGIN      
        1 tgggctacct gatgcggacg atcaccctgc tcggcgcgac cggttcgatt ggttcgagcg
       61 cgctcgatgt cattgcgcgc caccccgatc gttaccgcgt gcatgcactg acggcccagt
      121 ccagcgtcga tgcccttgcc gatctggcgc tgcgctttcg tccggcgctg gtggtcatcg
      181 gcgatgcaac actggagccg gtcctgcgcg ctgcattgcg tgaacgcggc gcaactgccg
      241 aaccccgcgc aggcgcggcg gcactggcgg aagtcgcgca ggcgccggaa gtcgataccg
      301 tgcttgcctc catcgtcggc tcggcggggc tggtgccaac actggctgcc gcgcatgcgg
      361 gcaaacgcct gctgctggcc aacaaggaag ccattgtctg tgctggcggt ctgctgatgg
      421 cggcactgcg cgcggcgggc ggcgaactgc tcccggtgga cagcgaacac aacgccattc
      481 atcaatgtct tgccgggacg ggcgagggcc agcgcggtcg ggcgcgcctc gtcctgacgg
      541 cctccggcgg gccattcctg cggcgacgcg atctcgatgc cgtgacgccc gacgaagcct
      601 gcgcgcatcc gaactgggtc atgggccgca agatctcggt cgactcggca acgctgatga
      661 acaaagggct cgaagtgatc gaggccagtt ggctcttcga catgccgccc gagcggatcg
      721 acgtcgtgat ccatccgcag agcgtgattt attcgatggt cgagtttccc gacggctcgg
      781 tgctggcgca gctcggttcg cctgacatgc gcacaccgct ggcctacgcg ctggcgtggc
      841 ccgaccgggt ggaaagcgga gccaaacggc tcgacttcgc gcgtctcggc tcgctcacgt
      901 tcgaagcgcc ggaccgggag cggttcccct gcctcggcta tgcctatgca gcgttgcgca
      961 gcggcggcgc ggcgagcgcg gtattgaacg cagccaacga gtatgcggtc gaagccttcc
     1021 tgcagcggcg cttgccgttc acgcagatcg cgccgaccat agaacgcgtg ctcgacgagt
     1081 ttcagccgac ggcgccggca agtgtcgagg aagtgctcga gatcgatcgg caggcgcgcg
     1141 accgcgccgc gctggcggtt gccctgcacg cctgaggatt gctgatgaat ctgataacga
     1201 caattgctgc ctttcttgtg accctcggga tcctgatcgt gatccacgag ctgggtcact
     1261 attgggtggc tcgctggtgt ggtgtcaagg tcctgcgctt ttcaatcggt ttcggccggc
     1321 cgatcgcgcg ctgggtgcgc ggacccgacc gcaccgaatg ggtggtttcg gccctgccgc
     1381 tcggcgggta tgtccgcatg ctcgacgaac gcgatccgga cacgccggtg accgacgagg
     1441 accttccccg tgccttcaac cggcagccgg tcggcaagcg gtttgccatc gtgctggcag
     1501 gcccggtggc caacctgctg ctggcgatct tcgtttactg gctgctgaac gtcatcggcg
     1561 tgtttgagcc gcgtgcgacg ctcggcgcgc cggccgccgc gaccgcagcg gccaccgccg
     1621 gggtcgaggc gggggacaca gtgacggcag tcgacggcga cggcgtgcgt tcgtggaacg
     1681 aactgcgctg gttgttgttg caaagggcgg tcgcccgtgc acccattgag atcgaggtcg
     1741 agggtgtgga cgggcagcgt cggacactga tcctcgaggc gggaccgctg accgcggccg
     1801 atcttgaatc cgatgtcgtc aatcgacttg gattcattcc ctacagcggt ccgccgcgag
     1861 tcaaccgact gaccgaagac ggcgcggccg caaggggcgg gctccggccg ggcgacacga
     1921 tcatcgaagc caatggcgcg aaggtcggct ccgcgcgcga cctgatcgag cgcatccgtt
     1981 cttccgcagg caagtcgatg gacctcggcg tgcaacgcga aggggaggtc gtgcgcctga
     2041 ccgtgacgcc agcactcgtc aacgatgagc agggcaagtc gatcggtcgc atcggcgccg
     2101 agctgaacga ccggcctccc ctggtcaagg ttcagtacgg accctttgaa agcatcggat
     2161 tagcgaccgg caagacctgg gacaccgcga tcttctcgct caagatgctg ggcaagatga
     2221 tcaccggcga agcgtcatgg aagaaccttt ccgggcccgt gaccattgcg gactacgctg
     2281 gacagaccgc gcgcatcggc cttgcaccgt tcctcaactt tcttgcattg gtttcaatca
     2341 gccttgcggt gctgaacctg ctcccggttc cgatgctcga cggagggcac ttactgtatt
     2401 atttgatcga aattctcaag ggcagcccgc cggccgattg ggtcatcgag tgggggcagc
     2461 gtgccggagt ggcactgctg ggcctgctga ccatgctggc gctctacaac gacctgatgc
     2521 ggctgctttc ctgatccccg gcatgattgg aaactgagtt ggatcgccgc gtgtcatccg
     2581 ggcgcagcgt gcccgtggcg ttgcgccgcg tcgcggcggc ggtcatcgct gcctattgct
     2641 tcagtgcgca ggcgcaggag ttcgtcgttc gcgacatccg cgtcgaaggt gttcagcgca
     2701 ccgaagccgg cactgttttc acctacctgc cgatccgtgt cggcgaccgc ttcgatcccg
     2761 agcgcggcac gcaggccatc cgtgccctgt acgccagcgg cctgttcaag gatgtccggc
     2821 tcgaagtcga cggtgatgtg ctggtcgtca tcgtcgatga gcgtccggcc atcgcgacga
     2881 tcgatctcgc cggagtgaag gagttcgaga aagaccaggt gctcaagtca ttgcgtgaag
     2941 tcgggctggc cgacgggcgc atctacgacc gggccctgct cgagcgcgcc gagcaggaac
     3001 tgcgtcgtca gtacctctcg cgcggacttt acggagtgga aatcaagacg acagtcacgc
     3061 cgatcgaacg caaccgggtc aatatctcgg tcgccgtgga agagggcgac acttcgaaga
     3121 tccgtacgat cgccttcacc ggcaacaagg tattcagcga tggcaccttg cggtcgcaga
     3181 tggacctgtc gacgccgaac tggatctcct ggtacaccaa gcgcgaccag tattcgcgtc
     3241 agaagctcgg tgccgacctc gaatcg
//
LOCUS       DATIRT010000410         4207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1545833, whole genome shotgun sequence.
ACCESSION   DATIRT010000410 DATIRT010000000
VERSION     DATIRT010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1545833"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1079)
                     /locus_tag="VLN25_09215"
     CDS             complement(69..1079)
                     /locus_tag="VLN25_09215"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HSQ10780.1"
                     /translation="MQRVLLILAVSITAGLGLADVRAQVASPHAISIPGWFAETFLDF
                     REDIAEARRDGKRLMIYFGQDGCPYCKLLMRTTFTETRVVDKARKNFVPIALNLWGDR
                     EVTWIDGRRMSEKELGRLLQVQFTPTLLFFDEKAGVIVRLNGYQPPNRMEAALDYVAG
                     RLEAKETLAAYMQRTVKDAASPTLHDEPFFVRPPYDLARSGSRPLLVVFERAPCASCD
                     ELHREGFRRKEVADQLARLTVARFDPASHVELTAPDGRRTTASAWARELRVTFAPTLV
                     FFDRGVEVFRVEAYVRPFHLATALEYVTTGAYRSEPSFQRHVQARADRLRARGEDVDI
                     WK"
     gene            1292..2059
                     /gene="hisF"
                     /locus_tag="VLN25_09220"
     CDS             1292..2059
                     /gene="hisF"
                     /locus_tag="VLN25_09220"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HSQ10781.1"
                     /translation="MLAKRIIPCLDVTAGRVVKGVNFVSLRDAGDPVEIARRYDGEGA
                     DELTFLDITASSDQRDIILHVIEAVAEQVFIPLTVGGGVRRVEDVRRLLNAGADKISI
                     NTAAVQDPQLVADASGRYGAQCIVVAIDARRRDGNATSWEVYTHGGRTPTGLDAVEWA
                     RRVAHLGAGEILLTSMDRDGTRQGFDLDLTRAVADAVSVPVIASGGVGTLQHLADGVT
                     RGSADAVLAASIFHYGEFTVREAKEYMAAQGVLMRLS"
     gene            2056..2475
                     /gene="hisI"
                     /locus_tag="VLN25_09225"
     CDS             2056..2475
                     /gene="hisI"
                     /locus_tag="VLN25_09225"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HSQ10782.1"
                     /translation="MSQTLSRDAGEGPAWLNEVNFDAADLVPVIAQDASSGRILMVAW
                     ANREALVETAQTRRGVYWSRSRGRLWRKGEESGHAQQVGEIRLDCDGDVVLYAVEQVG
                     GIACHTGRQSCFFRRLGDDGWDVVDPVLQDPELIYRK"
     gene            2472..2834
                     /locus_tag="VLN25_09230"
     CDS             2472..2834
                     /locus_tag="VLN25_09230"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HSQ10783.1"
                     /translation="MNDAILSQLAAVIESRRGTDPDVSYVARLLARGEDAILKKIGEE
                     ATEAVMAAKDGSPERIIAETADLWFHCLIMLAHYGLQPGQVLDELARRAGTSGIEEKA
                     SRKAMEREAHDNVKGSGG"
     gene            2831..3175
                     /locus_tag="VLN25_09235"
     CDS             2831..3175
                     /locus_tag="VLN25_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HSQ10784.1"
                     /translation="MTEKGCLFCRIARGEVPSRKAYEDDQILVFHDINPAAPVHLLVI
                     PKVHVDSLVTVGPEHEALLGKLLTLAPQLAKEQGCDNGFRVVINNGPDGGQEVNHLHI
                     HILGGPRPWKRL"
     gene            3185..3406
                     /gene="tatA"
                     /locus_tag="VLN25_09240"
     CDS             3185..3406
                     /gene="tatA"
                     /locus_tag="VLN25_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HSQ10785.1"
                     /translation="MGSFSIWHWLIVLVVIILVFGTAKLKNVGKDLGGAIKGFKEGMK
                     EGTDEVAQKVEDTKKVAGQTIDVQAKEKS"
     gene            3484..4008
                     /gene="tatB"
                     /locus_tag="VLN25_09245"
     CDS             3484..4008
                     /gene="tatB"
                     /locus_tag="VLN25_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HSQ10786.1"
                     /translation="MFDFSFGELALIGAVALVVLGPERLPKVARTVGEWAGKAQRYVS
                     QVKSDINREMELSELKKLQEQARGIAHEFETSVKNEVKGIETSVTSGMPSLSSGADIA
                     SLGSDTGRDNWGDTNVWTSTRFNRRYKPGPSIDDLSEEIAKLRRRLALPETTGHSRHK
                     YAPRARVNRARIRR"
BASE COUNT          763 a         1352 c         1402 g          690 t
ORIGIN      
        1 cgatcgactt caagcgcgcc caggaacgcg ccgacgaact cgccggcgag tccgagtagg
       61 cacccgctct acttccagat atcgacgtcc tcgccgcgcg cccgcaaccg gtcggcgcgc
      121 gcctgtacgt gacgctggaa actcggctcc gagcggtacg ccccggtcgt cacgtattca
      181 agcgccgttg ccagatggaa cggacggacg taggcctcga cgcggaacac ctcgacgcca
      241 cgatcgaaga agaccagtgt cggtgcaaag gtgacacgca attcacgcgc ccaggccgac
      301 gccgtcgtgc ggcgcccgtc gggcgcggtg agttcgacgt ggctggccgg atcgaagcga
      361 gcgacggtca ggcgcgcaag ctggtcggcc acttccttgc gccggaatcc ttcgcgatgc
      421 agttcgtcac aactggcgca cggcgcgcgc tcaaagacga ccagcagcgg ccggctgccg
      481 gatcgcgcga gatcgtaggg cgggcgcacg aagaaaggct cgtcgtgcaa tgtcgggctg
      541 gccgcatcct tcacggtccg ctgcatgtac gccgcgagcg tttccttcgc ttcgagtcgg
      601 ccggcaacgt agtcgagcgc ggcttccatg cggttgggtg gctgataccc gttcaggcgc
      661 acgatcactc ccgccttctc gtcgaagaac agcagggtgg gagtgaactg cacctgcagg
      721 aggcgcccga gttccttctc ggacatgcgg cgaccgtcga tccaggtgac ttcgcgatcg
      781 ccccagaggt tgagtgcgat cgggacgaag ttcttccgtg ccttgtcgac gacgcgggtc
      841 tcggtgaacg tcgtccgcat cagcagcttg caataggggc acccgtcctg gccgaagtag
      901 atcatcaggc gcttgccgtc ccggcgcgcc tcggcgatgt cctcgcggaa gtccaggaag
      961 gtctcggcaa accagcccgg gatgctgatc gcgtgcggcg aggcgacctg cgcgcgcacg
     1021 tccgctaggc ccagaccggc agtgatgctg accgcgagta tcagcaggac tcgttgcacc
     1081 gaggcgactc cgcagggcat tgaagtgcgc agttcaacac ggacacgcga atgcgcggac
     1141 gcggggcccg cacgacagtt tcagccgagc ccggtggtcg cggaaccccg cgatcgggtc
     1201 acccggagca accctaggcg aagcacgctg gcgccacctc ggggcgtcag catcgtgttg
     1261 cgctagagtc cgctagctgg aatccgacct catgctggcc aagcgcatca ttccctgcct
     1321 cgacgtgacc gccggccgcg tggtcaaggg cgtcaacttc gtcagcctgc gcgacgccgg
     1381 cgatccggtg gagattgcgc gccgctacga cggcgagggc gccgatgaac tcaccttcct
     1441 cgacatcacc gcgagctccg accagcgcga catcatcctg cacgtgatcg aagcggtcgc
     1501 cgagcaggtc ttcatccccc tcacggtggg cggaggcgtg cgccgggtcg aggatgtccg
     1561 ccgcctgctg aacgcaggtg ccgacaagat ctcgatcaac acggcggcgg tccaggaccc
     1621 gcaactcgtg gcggacgcaa gcgggcgcta cggcgcacaa tgcatcgtgg tggcgatcga
     1681 tgcccgccgc cgggacggca atgccacctc ttgggaggtc tatacccacg gcggccgcac
     1741 gccgaccggt ctcgacgcgg ttgaatgggc gcgtcgtgtc gcgcatctgg gcgccggcga
     1801 aatcctgctg accagcatgg accgcgacgg cacccgccag ggcttcgatc tcgatctgac
     1861 gcgcgcagtg gccgatgcgg tgtctgtccc ggtgattgcc tccgggggtg tcggcacgct
     1921 ccagcacttg gccgacggtg tcacgcgcgg cagcgccgac gccgtgctgg cagcatcgat
     1981 ctttcattac ggcgagttca ctgtgcgcga ggccaaggag tacatggccg cgcagggtgt
     2041 gctgatgcga ctgtcatgag ccagaccctg tcccgagatg ccggagaagg cccggcctgg
     2101 ctcaatgagg tgaacttcga tgccgcggac ctggtcccgg tcatcgccca ggatgcctcc
     2161 tccgggcgaa tcctgatggt tgcctgggcc aatcgcgagg ccttggtcga gaccgcgcag
     2221 acacgccggg gggtttactg gtcgcgctcg cgcggacggc tatggcgcaa gggcgaggag
     2281 tccggacacg cccagcaggt cggcgagatt cgccttgatt gcgacgggga tgtcgtcctg
     2341 tacgctgtcg aacaggtcgg cgggatcgct tgccacacgg ggcggcagag ctgctttttc
     2401 cggcgactgg gcgatgacgg ctgggacgtc gtcgacccgg tgctgcagga ccctgaactc
     2461 atctaccgca aatgaacgac gcgatcctga gccaactggc cgccgtcatc gagtcccgtc
     2521 gtggcaccga tcccgacgtg tcctacgtgg cccgtctgct tgcgcgcggc gaagatgcca
     2581 tcctcaagaa gatcggcgaa gaggcgaccg aggctgtcat ggcggcgaag gacggctccc
     2641 ccgaacggat catcgccgag actgcggacc tctggtttca ttgcctgatc atgctcgccc
     2701 actacgggct gcagccggga caggtgctgg acgaactcgc acgccgcgcc ggcacctcgg
     2761 ggatcgaaga gaaggcgtca cgcaaggcca tggaacgcga ggcgcacgac aacgtcaaag
     2821 gatcgggtgg atgaccgaaa aggggtgtct tttctgccgc attgcccgcg gcgaggtgcc
     2881 gagccgcaag gcctacgagg acgatcaaat cctcgttttt cacgatatca atcccgcggc
     2941 gccggtgcat cttctggtca ttccgaaggt gcacgtggat tcgctggtca cggtcgggcc
     3001 ggagcacgag gcactgttgg gtaaactgtt gacccttgcg ccgcagctcg cgaaagagca
     3061 aggctgcgac aacggctttc gtgtcgtgat caacaacggc ccggacggcg gacaagaggt
     3121 caatcacttg catatccaca tcctgggcgg tccgcgcccg tggaagcgtc tgtaggagtt
     3181 cacgatggga tcattttcga tctggcactg gctgatcgtc ctcgtagtca tcatcctggt
     3241 tttcggtacc gcgaagctga agaacgtggg caaggacctg ggtggtgcga tcaagggatt
     3301 caaagaaggc atgaaggaag gcactgacga ggtcgcccag aaggtcgagg acaccaagaa
     3361 ggtcgccggc cagacgatcg acgtgcaggc caaagaaaag tcctgattgc ccgggtcgcc
     3421 ccccgcgccc gttgcggccg gggcgcactc atgcttcgcc tctcgacctg acggccgccc
     3481 tccatgttcg acttcagctt cggcgaactg gcactgatcg gcgccgttgc actggttgtg
     3541 ctcggccccg aacggctgcc gaaagtcgcg cgcaccgtcg gcgagtgggc cggcaaggcg
     3601 cagcgctatg tcagccaggt gaagtccgac atcaaccgcg agatggaact gtcggagctg
     3661 aaaaagctgc aggagcaggc gcgcggtatc gcgcacgagt tcgaaacctc ggtgaagaac
     3721 gaggtcaagg gcatcgagac gagcgtgacc tcggggatgc cgtcgctatc gagtggcgcc
     3781 gacattgcca gcctcggctc cgacactggc agggacaact ggggtgatac gaacgtctgg
     3841 acgtcgacgc gcttcaaccg acgctacaag ccgggaccca gcatcgacga cctgtcggaa
     3901 gaaatcgcca agctcaggcg ccggctcgct ctgccggaaa cgaccggtca ttcgcgccac
     3961 aagtacgcgc cgcgcgcccg cgtcaaccgt gcgcgtatcc ggcgctgatc ccacctcgcg
     4021 ctgccgtcga tgacgaccga aaccaacgag cccggccagg aacagagttt cctgacgcac
     4081 ctggtcgaac tgcgtacgcg gctggtccgc gccgcgctgg caatcgtcat cgtgttcgcg
     4141 gcgttgacac ccttcatgac ggagatcttc gatctgctgt cgcggccgat gatggcggcg
     4201 ctgccgg
//
LOCUS       DATIRT010000411         3797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_955461, whole genome shotgun sequence.
ACCESSION   DATIRT010000411 DATIRT010000000
VERSION     DATIRT010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955461"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="VLN25_09250"
     CDS             complement(<1..835)
                     /locus_tag="VLN25_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HSQ10787.1"
                     /translation="MFAKAAIWMVIALVLFTVFKQFESRQKPAETVSYTQFLEEAKAG
                     KIKRVEVQGRTLQVTPTEGTRYTLTSPGDAWMVSDLMKSGVQVFGKPEEEQSLLTTIF
                     VSWFPMLLLIGVWIFFMRQMQGGGRGGAFSFGKSRARMLDESNNSITFVDVAGCDEAK
                     EEVQELVEFLRDPSKFQKLGGRIPRGVLLVGSPGTGKTLLAKAIAGEAKVPFFSISGS
                     DFVEMFVGVGAARVRDMFEQAKKHAPCIIFIDEIDAVGRQRGAGLGGGNDEREQTLNQ
                     ML"
     gene            complement(1015..1692)
                     /locus_tag="VLN25_09255"
     CDS             complement(1015..1692)
                     /locus_tag="VLN25_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HSQ10788.1"
                     /translation="MPQAKKKHRSNPAWIKRHVTDPFVQRSVQDGFRSRAAYKLSEIA
                     DTDKLLKPGMTVVELGAAPGSWTQVVRQRLQARDGTLRGRIVALDILPMDPVGGVDFL
                     EGDFREEAVEEQLAALLQGEQVDAVLSDMAPNLSGIAAADAARSLHLAELAADFACKH
                     LKPDGVFLVKAFQGSGYSQFVERLKREFRTVSTRKPAASRESSAEVFLLARGLRGTAA
                     SPRGQTA"
     gene            1729..2289
                     /gene="yhbY"
                     /locus_tag="VLN25_09260"
     CDS             1729..2289
                     /gene="yhbY"
                     /locus_tag="VLN25_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016475064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HSQ10789.1"
                     /translation="MPEIQLTPKERQELKARAHGLKPVVLLGNSGLSAPVMKEIDRAL
                     AAHELIKVKVPGEDREERDTLFADVAEALSAARVQSIGKLLVLYRPAPEEKEEAVTRP
                     VRKRATVDDRSPKAPARRVGPALRAKVPGKPSAPKRSTPDRRDAERVNRGSAPRRDPG
                     ASTKAAGRTNVARRGVGRGGGGGKAG"
     gene            complement(2290..2763)
                     /gene="greA"
                     /locus_tag="VLN25_09265"
     CDS             complement(2290..2763)
                     /gene="greA"
                     /locus_tag="VLN25_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HSQ10790.1"
                     /translation="MPIPITARGAEKLKAELQRLKAVDRPTVIQAIKDAREKGDLSEN
                     AEYDAARERQGFIEGRIQELEGKVSSLQIIDPTTVDAAGRIVFGATVELEDQQSGEVV
                     KYQIVGDDEADIKAGMISVSSPIARALIGRSEGDMAQVVAPAGMREYAVVSVAYQ"
     gene            complement(2867..>3797)
                     /gene="carB"
                     /locus_tag="VLN25_09270"
     CDS             complement(2867..>3797)
                     /gene="carB"
                     /locus_tag="VLN25_09270"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522408.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HSQ10791.1"
                     /translation="RVLIGGVMEHIEQAGVHSGDSACSLPPYSLTPETIAELKRQTEQ
                     MARALNVVGLMNVQFAIQAGNDGQGTIYVLEVNPRASRTVPFVSKATGMPLAKIAALA
                     MVGISFEKQGEVREIVPPYYSVKEAVFPFAKFQGVDPLLGPEMRSTGDVMGVGETFGE
                     ALFKSQLGAGASLPDKGAVFLSVKDQDKAKVVALAQLLHDMGYELVATRGTAQAVEQA
                     GIPVKTVNKVKDGRPHVVDLLKNGDIQLVITTVSEVRSQIADSRSIRTTALAQRVTYY
                     TTMAGGRAAIEGMKHRDHLEVYDLQGLHRRLQH"
BASE COUNT          669 a         1269 c         1190 g          669 t
ORIGIN      
        1 ccagcatttg gttcagcgtc tgttcgcgct cgtcgttgcc accgcccagg ccagcgccac
       61 gctgacggcc gacggcgtcg atttcatcga tgaagatgat gcagggcgca tgcttcttgg
      121 cctgctcgaa catgtcgcgc acgcgcgccg cgccgacacc gacgaacatc tcgacgaagt
      181 cggaacccga gatcgagaag aacgggacct tggcctcgcc ggcgatcgcc tttgcgagca
      241 gggtcttgcc ggtgccgggc gagccgacca gcagcacgcc gcgcgggatg cggcccccga
      301 gcttctgaaa cttggacggg tcgcgcagga actcgaccag ttcctggact tcttccttcg
      361 cctcgtcgca gccggcgacg tcaacaaagg tgatcgaatt gttgctctcg tcgagcatgc
      421 gtgctcgcga cttgccaaag ctgaatgctc cgccacggcc gccaccctgc atctgccgca
      481 tgaagaagat ccagacaccg atcagcagca gcatcgggaa ccacgagacg aagatcgtcg
      541 tcagcagcga ctgctcttcc tcaggcttgc cgaagacctg gacgccggac ttcatcaggt
      601 cgctgaccat ccaggcgtcg cccggcgacg tcagcgtgta gcgcgtgccc tcggtcggcg
      661 tgacctgcag cgtgcggccc tgcacttcaa cgcgcttgat cttgccggcc ttcgcttcct
      721 ctaggaactg cgtgtagctg acggtttccg ctggcttctg gcggctttcg aactgcttga
      781 agacggtgaa caggacgagc gcgatcacca tccagatggc ggctttcgcg aacatgttgt
      841 tgttcaagga aggctcctaa tggccgcgct ggtatcggaa agctcgtcgg ccgggaaatc
      901 gggtcgggat caaatcggtt tgggacctgc gtttcgagcc attctaatcc tcgggcccgc
      961 tcacgcagga gaagttgggg gcgaaggggg ggctatttca ataccctcag gacactacgc
     1021 cgtttggccc cgtggagaag cagccgttcc gcgcaggccg cgcgccagca gaaacacttc
     1081 ggccgacgat tcacgtgacg ccgccggctt gcgcgtcgac acggtacgga attcccgctt
     1141 caggcgttcg acgaactggc tgtatccact gccctgaaag gctttgacga gaaaaacgcc
     1201 atctggtttc aagtgtttgc acgcaaagtc ggccgccagt tccgccagat gcaggctgcg
     1261 tgcggcgtcg gccgcggcaa ttcccgaaag gttgggcgcc atgtcggaca ggaccgcgtc
     1321 gacctgctca ccctgcagca acgccgccag ctgctcttcc accgcctcct cacggaagtc
     1381 gccctcgagg aagtccacac cccctaccgg gtccatcggc aggatgtcga gcgcgacgat
     1441 gcggccccgc aacgtcccgt cgcgtgcctg cagtcgctgg cgcacgacct gggtccagct
     1501 accgggggcg gcacccagtt cgactaccgt catgcccggc ttcagcaact tatcggtgtc
     1561 agcgatctcg gacagcttgt aagcggcgcg cgaacggaac ccgtcctgga ccgagcgctg
     1621 tacgaacggg tccgtgacgt ggcgcttgat ccacgccggg ttggagcgat gtttcttctt
     1681 cgcctgcggc atccgtgact acactgaagc tcaaaaggag agatgactat gcccgagatc
     1741 cagctgaccc ccaaggaacg ccaggaactg aaggcgcgcg cccatggact caagcccgtc
     1801 gtccttctgg gcaactccgg gctgagcgcc ccggtgatga aggaaatcga tcgcgccctc
     1861 gctgcgcatg agctgatcaa ggtgaaggtg ccgggcgaag accgggaaga acgcgatacg
     1921 ctgttcgcag acgtcgcgga agcactttca gccgcgcgcg tgcaatcgat cggcaagctg
     1981 ctggtgctgt accgccccgc gcccgaggag aaggaagaag cagtcaccag gccggtacgc
     2041 aaacgcgcca cggtcgacga ccgttcgccc aaggctcccg cgcgccgggt cggaccggca
     2101 ctgcgggcca aggtacccgg gaagccctcg gcaccgaagc gctcgacgcc cgaccgccgt
     2161 gacgccgaac gcgtgaatcg tggcagcgca ccgcggcgcg atcccggcgc ctcgacaaag
     2221 gctgccgggc ggaccaacgt ggcacggcgc ggagtcggac gcggtggcgg gggaggaaag
     2281 gccggctagt tattggtagg cgaccgagac caccgcgtat tcgcgcattc cggccggtgc
     2341 gacgacctgc gccatgtcgc cttcgctcct gccaatcagc gcacgggcga tcgggctgga
     2401 tacggagatc atgcccgcct tgatgtcggc ctcgtcgtcg ccgacgatct ggtacttgac
     2461 cacctcgccc gactgctggt cctcgagttc gacagtcgcg ccgaagacga tgcgtccggc
     2521 cgcgtcgacc gtggtcgggt cgatgatctg caggctcgag accttgcctt cgagttcctg
     2581 gatccgccct tcgatgaagc cttgacgttc gcgcgccgcg tcgtactcgg cgttctcgga
     2641 cagatcgcct ttttcgcgtg cgtccttgat cgcctgaatg accgtcggcc gatcgacggc
     2701 cttcagacgc tgcagttcgg ccttcagttt ttcggcgccg cgcgccgtga tgggtatggg
     2761 cattgctcgc aggaactcgt gggaaaaaca aagccccggc aatgccgggg ccgctgagcc
     2821 actctagcca aagcgccgcc gcagcgcaac ccgccgccag tcggccctaa tgctgcaggc
     2881 gacgatgcag gccctgcagg tcgtagacct caagatgatc gcggtgcttc attccttcga
     2941 tcgcggcgcg cccgcctgcc atggtcgtgt agtacgtcac gcgctgtgcc agcgcggtcg
     3001 tgcggatcga ccgggaatcc gcgatctgac tgcgcacctc gctcacggtg gtgatcacga
     3061 gctgaatgtc gccgtttttc agcagatcga ccacgtgcgg ccgcccgtcc ttgaccttgt
     3121 tcacggtctt caccgggatg ccggcctgct cgaccgcctg ggcagtgccg cgcgtcgcca
     3181 cgagctcgta gcccatgtcg tgcaggagtt gcgccagcgc gaccaccttg gccttgtcct
     3241 ggtccttgac gctcaggaac acggcccctt tgtccggcaa ggacgcgccc gcgccgagct
     3301 gcgacttgaa cagggcctcg ccgaaggtct cgccgacgcc catcacatcg ccggtcgaac
     3361 gcatctccgg ccccagcaac ggatcgacgc cctggaactt ggcaaacggg aagaccgctt
     3421 ccttgaccga gtaatacgga ggcacgatct cgcgtacctc cccttgcttc tcgaaggaaa
     3481 tgccgaccat cgcgagggcg gcaatcttcg ccagcggcat gccggtcgcc ttggacacga
     3541 acggcacggt acgagaagca cgcggattga cctcgagcac gtagatcgtg ccctgcccgt
     3601 cattgcccgc ctggatcgcg aactgcacgt tcatcagccc caccacgttg agtgcgcggg
     3661 ccatctgctc ggtctggcgc ttgagttccg caatcgtctc gggcgtgagc gagtacggcg
     3721 ggagggagca cgccgagtca cccgagtgca cgccggcctg ttcgatgtgc tccatgacgc
     3781 caccgatcag cacccgt
//
LOCUS       DATIRT010000412         3327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1349057, whole genome shotgun sequence.
ACCESSION   DATIRT010000412 DATIRT010000000
VERSION     DATIRT010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1349057"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /gene="ccsA"
                     /locus_tag="VLN25_09275"
     CDS             <1..631
                     /gene="ccsA"
                     /locus_tag="VLN25_09275"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HSQ10792.1"
                     /translation="HALSATLWLTVLIVWAESYFSASRGLLLLVLPMAAVFSGLPVIF
                     PGSVLGPATASVVFRLHLLLAILAYSLLTIAAVQALLMATMDRRLHGDLMTERTPLAR
                     FLNRLPPLLAMETVLFRLITAGFVLLTATLVSGVFFSEQTFGRPLRLDHKSVFTIAAW
                     LVFGGLLVGRLSFGWRGRTALRWTLIGFAMLMLAYVGSRFVMEVLLGRV"
     gene            631..864
                     /locus_tag="VLN25_09280"
     CDS             631..864
                     /locus_tag="VLN25_09280"
                     /inference="COORDINATES: protein motif:HMM:NF041023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HSQ10793.1"
                     /translation="MGRIVFFLLLAVAAYVGWRWLRAASDRTASTRSDKVPAPQAMVS
                     CAHCGLHLPRLDALPAGDRFFCSEEHRHRGPSS"
     gene            874..2580
                     /locus_tag="VLN25_09285"
     CDS             874..2580
                     /locus_tag="VLN25_09285"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSQ10794.1"
                     /translation="MSALEPTVQEPVTSEQDDAPWRTLEYFATTRVIVASALVLGAAA
                     LGVRPSGLAAGVHSGLLMSLAYFGASAVFAGLALYLHRHFMPQVFGQLALDLAAVTTL
                     VISGGGVASGWVILYLLPLAGASLLLPSLQVFFVCSIAVLAILIDAGLRTFAPEGSDS
                     LLFQAGIFGAALFAVSALLRALSARLASQEKLARSRGRDLQNQLAINRLVIAQMDEGV
                     IVVDRTGRVRANNRAARRMLGLNSTAQLTGECLADVPGASELAVAFSAWSEGGRSEGL
                     WSNTIVPTGENGAAVAPESRLRARFARPTSPSTDEFVIFIEDVRDVEDRAQQLKLAAM
                     GRLTASIAHEIRNPLAAISNAGQLLNEDARDPVLKRLAAIVRENTHRLNRLVEDVLRV
                     ARREPPLGDEISLSDFVREFLAEFVRDRGLAASTIEAQLEPDQRVKFEQSHLRQVLYN
                     LVDNALRYASGGSGCVCLVVEPAPEGDRPQLWVLDDGPGVPFEARTGLFEPFFTTRNQ
                     GTGLGLYLAREFCVTNRADLTYASRREVDGSSRDGFLLRFGLGSKADASLHGFLDTIP
                     VR"
     gene            2577..>3327
                     /locus_tag="VLN25_09290"
     CDS             2577..>3327
                     /locus_tag="VLN25_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HSQ10795.1"
                     /translation="MNEPLHSETTRPAPARRSPAVLVVDDEADLRELLALTLVRLGLD
                     VDTAESVSAARTLLAQNHYALCLTDMRLPDGTGLELVREVAQSSGPPIAVITAYGSAE
                     NAVAALKAGAFDYLTKPVDLDQLRHLVRSTVRNATGSAVSDTDARSAESLPALARLVG
                     TTPTMQQMRALIERLARSMAPVAICGESGSGKELAARAIHDLSARRSQPFVAVNCGAI
                     PETLMEAEFFGYRKGAFTGADRDRDGFFQAAA"
BASE COUNT          488 a         1056 c         1124 g          659 t
ORIGIN      
        1 gcacgcgttg tcggccaccc tctggctgac cgttctgatc gtctgggccg aaagctattt
       61 ctcggcgtca cgcggactgc tgttgctggt gttaccgatg gcagcggtgt tctcaggttt
      121 gccagtgatt tttcccggat cggtgctggg tccggcaacg gcgtccgtcg tgtttcgcct
      181 gcacctgttg ctggccatac tcgcctacag cctgctgacg atcgctgcgg tgcaggccct
      241 gctgatggcc acgatggacc gccgccttca cggtgatttg atgaccgagc gtacgcccct
      301 ggcgcgtttc ctcaatcgcc tgccgcccct gctcgcgatg gaaacggttc tgttccggct
      361 gatcaccgcc ggcttcgtgt tgctgactgc aacgctggtg agcggggtct ttttctcgga
      421 acagaccttt ggccggccgc tacgcttgga ccacaagtcg gtgttcacga tcgccgcatg
      481 gctggtgttc ggcggcttgc ttgtagggcg gctctccttc ggttggcgcg gccgcacggc
      541 attgcgctgg acgctgatcg gcttcgccat gctgatgctg gcgtacgtcg gcagccgatt
      601 tgtcatggag gtcctgctgg gacgcgtgtg atgggacgca tcgttttctt tcttctgctt
      661 gcggtcgcag cctatgtcgg gtggcgatgg ctgcgcgctg catcggaccg cacggcatcg
      721 acacgcagcg ataaggtgcc ggcaccgcaa gccatggtca gctgcgccca ttgcggcctg
      781 catctgccgc ggctggacgc cctgccggcc ggcgatcgct tcttctgttc tgaagagcat
      841 cgtcaccgcg gtccgtcgtc ctgaacgtct ctgatgtccg cgctcgagcc gacagtccag
      901 gaacccgtta ccagcgagca ggacgatgcg ccatggcgca cgctcgagta ctttgcgacg
      961 acacgcgtga tcgtcgccag tgccctcgtt ctgggcgccg cagccctcgg cgtccggcca
     1021 tccgggctgg ccgccggcgt gcactcgggg ctattgatgt cgctggccta cttcggtgcg
     1081 tcggcggtct tcgccggcct ggcgttgtac ctgcaccggc acttcatgcc gcaggtgttc
     1141 ggacaactag cgctcgatct ggcggccgtg accaccctgg tcatttcggg cggcggcgtg
     1201 gcgtcgggct gggtgatcct gtatctgctg ccgctggctg gcgcgtcgct gctgctgccg
     1261 tccctgcagg tcttcttcgt ctgttccatt gcggtgctgg cgatactgat cgacgccgga
     1321 ttgcgcacct ttgccccgga aggaagtgac tcgctgctgt tccaggccgg cattttcggc
     1381 gcagcgttgt ttgcggtgtc ggcactgctg cgcgcgctca gcgcgcgcct ggccagccag
     1441 gagaagctgg cccgaagccg tggccgcgac ctgcagaatc agctggcgat caaccgtctc
     1501 gtcattgccc agatggacga aggcgtcatc gtggtcgatc gcacgggtcg cgtgcgtgcg
     1561 aataaccggg cggcgcggcg catgctgggt ctgaactcga ccgcacagct gaccggcgag
     1621 tgtctggcgg acgtacccgg agcgagcgaa ctggcggtcg cattttccgc ctggagtgaa
     1681 ggcggccgca gtgaaggcct gtggtcgaat acgatcgtac cgaccgggga aaacggcgcg
     1741 gcggtagcgc ccgagtcgcg gctgcgggca cgctttgcac gacccacgtc gccgtcgacc
     1801 gacgagttcg tcatcttcat cgaggacgtg cgcgatgtgg aagaccgggc gcagcaactc
     1861 aagctcgccg cgatgggacg gctgacggcc tccattgcac acgagatccg caacccgctg
     1921 gctgcgatca gcaacgccgg ccagttgctg aacgaggacg cgcgcgatcc ggttctcaag
     1981 cgcctcgcgg cgatcgtgcg cgagaacacg caccggctga atcggctggt cgaggacgtg
     2041 ttgcgcgtcg ctcgtcgtga gccgccgctg ggcgacgaga tctcactgtc ggacttcgtc
     2101 cgcgagttcc tggcggagtt cgtgcgcgat cgcggcctgg cggcatcgac gatcgaggcg
     2161 cagcttgagc ccgaccagcg cgtcaagttc gagcagtctc acttgcggca ggtgctgtac
     2221 aacctggtgg acaacgcgct gcgttacgcc agcggcggat ccggctgcgt ttgcctggtc
     2281 gtggagccgg caccagaagg tgatcgcccc cagctctggg tgctcgacga tggaccgggg
     2341 gtgccctttg aagctcgcac gggactgttc gaaccgttct tcactacacg caaccagggt
     2401 accgggctgg gcttgtacct ggcacgggag ttctgtgtca cgaatcgagc cgacctgacg
     2461 tatgcgtcac ggcgcgaagt cgacggcagc tcgcgcgacg gcttcctgct gcgtttcggc
     2521 ttgggctcaa aggccgatgc gagcctgcat ggcttcctgg atacgattcc ggtgcgatga
     2581 acgagccgct gcattcggaa acgacgagac cagcgccggc gcgccgatcc ccggccgtcc
     2641 tcgtcgtcga cgatgaggcg gacctgcgcg aactgttggc gttgacgctg gtgcgattgg
     2701 gtctcgacgt cgacaccgcc gagtcggtgt cggcggcgcg cacgctgctc gctcagaatc
     2761 actatgcgct gtgcctgacc gatatgcgat tgcccgacgg cacgggtctc gaactggtgc
     2821 gcgaagtggc gcagtcctca ggcccgccga ttgcggtgat cacggcgtat ggcagtgccg
     2881 agaacgcggt ggccgcgctg aaggcgggcg cgttcgacta cctgaccaaa ccggtcgacc
     2941 tggatcaatt gcgccacctg gttcgttcca ctgtgcgcaa tgcgacaggg agcgccgtat
     3001 ccgatactga cgcgcggagt gcagagtcac tgccggcact ggcccgactg gttggcacga
     3061 cgccaacaat gcagcagatg cgcgcgctca tcgagcgcct ggctcgcagc atggcgcccg
     3121 tggcgatctg cggagagtcg ggcagcggca aggaactcgc ggcccgagca atccacgacc
     3181 tgtcagcgcg gcgttcgcaa ccgtttgtcg ccgtgaactg cggcgccatc ccggagaccc
     3241 tgatggaggc ggagttcttt ggctatcgca aaggagcctt caccggcgcc gatcgcgatc
     3301 gcgacggatt ttttcaggcc gctgctg
//
LOCUS       DATIRT010000413         4757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_955592, whole genome shotgun sequence.
ACCESSION   DATIRT010000413 DATIRT010000000
VERSION     DATIRT010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955592"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VLN25_09295"
     CDS             <1..186
                     /locus_tag="VLN25_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HSQ10796.1"
                     /translation="PVGLMIFLLVPEQATQQHLDILSELAQMLSDKSFRETLLTASDA
                     NAVHVALTTWEPVRPAA"
     gene            269..1198
                     /gene="hprK"
                     /locus_tag="VLN25_09300"
     CDS             269..1198
                     /gene="hprK"
                     /locus_tag="VLN25_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HSQ10797.1"
                     /translation="MPTFQSLYDDNREPLRLSWIAGQGSAGKALDTPSTAGLASADLV
                     GHLNLIHPNRLHVLGEAEVRYYSQMEPSRRLHYASELIAGKPIGIVIGEGLTPPPELV
                     DTADRAGLPALASPQSAAEIIEVLRIYFAKVLAAHCTMHGVLMDVLGMGVLVAGESGV
                     GKSELGLELISRGHGLVADDVVDFSRVAPDTIEGRCPPLLANLLEVRGLGLLDIRSIF
                     GETAVRRKMKLRLIVELRRYGASETMERLPLEGQSQDVLGIPVRKVLIPVASGRNLAV
                     LTEAAVRNTILQLRGIDTMRDFLQRHQQHMNGN"
     gene            1321..2193
                     /gene="rapZ"
                     /locus_tag="VLN25_09305"
     CDS             1321..2193
                     /gene="rapZ"
                     /locus_tag="VLN25_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016475217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HSQ10798.1"
                     /translation="MRIVLVTGMSGSGKSVAIRLLEDAGYYCIDNLPSRFLRDVCAFL
                     DSTGHGDVALSVDSRSEASLGDIPSIIASLRGAGHDVRVLFLTASNAALVQRFSETRR
                     RHPMSIRVQPQGAEATLTESIELERELLAPLVEVGHVIDTSELHPNTLRQWVRQFTDL
                     PPAALVLAFESFAFKQGVPVAADFVFDVRNLPNPHYDPRLRPLTGRDAAVVAYLERIP
                     QAGEMLDDIAGFIGKWLPSYVADNRHYLTVAIGCTGGRHRSVYFVEELARRFRAQRNV
                     LVRHRAFEDSETGT"
     gene            2198..2836
                     /locus_tag="VLN25_09310"
     CDS             2198..2836
                     /locus_tag="VLN25_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HSQ10799.1"
                     /translation="MRLPLFPLSTVLFPGGVLPLRIFEARYMDMARDCLRDELQFGVC
                     MITDPKANEASRNPSVADIGCSASIAGWDMRQLGLLHVRAVGGQRFRVISSKTQGNGL
                     KVAEIEWVEDDVDAPIASDHVPCVDLLRRIIDDLRAQAAERRRESEDDDVLAQPPFEE
                     PFRMESSTWVSNRLCEVLPVPLKAKQKLMELGDGRARLEIVHTYLKQHSVFK"
     gene            complement(2829..3899)
                     /gene="mutY"
                     /locus_tag="VLN25_09315"
     CDS             complement(2829..3899)
                     /gene="mutY"
                     /locus_tag="VLN25_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HSQ10800.1"
                     /translation="MKFSDLLVAWQRRHGRHGLPWQGTRDAYRIWLSEIMLQQTQVAT
                     VVPYYLRFLARFPDVHALAVAPIDDVMSAWAGLGYYSRARNLHRCAQTIVGEHDGAFP
                     SSAAMLEQLPGIGRSTAAAIAVFAFGERAAILDGNVKRVFARHFGVGGYPGAVATERV
                     LWELVTAQLPRADIETYTQGLMDLGATICVRARPRCDACPVRSTCIALRDERIAELPA
                     PRPSRVRPLRAATLALITDSTGAILLERRAPTGIWGGLLSAPEFDAELSDAALQAAIA
                     KRFGLRGSVEQRLESVRHEFSHYSFLMHPRVVSATGAIGVAQAAGLEWLGADEVDHAA
                     LPAPLRRLLRTLADRVEDARTT"
     gene            complement(3987..>4757)
                     /locus_tag="VLN25_09320"
     CDS             complement(3987..>4757)
                     /locus_tag="VLN25_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10801.1"
                     /translation="RMTRRALAEQREFEEVVRKIVAARVVLQKLSDKLFVPLRVSTLR
                     AQVLATRNKMKKSQLSPSISGAVRDYFGSLRELLRDANAKALEIEQMVLGVQRRFAEE
                     LGWSLSPPMTFSLDTYIADLERTEAAYRSQFGAFAVLTRDRWTLMERFFETVVAKSRE
                     IFSAAERDTEAWVKSLLPPIELQVREQRAQLKKRAESISKIRDAQGSLDERISELELA
                     LEDTQARQAALRRFADRVHEVGARVPDELGLAEVPLAA"
BASE COUNT          819 a         1607 c         1523 g          808 t
ORIGIN      
        1 ccggtcgggc tgatgatctt cctgctggtg cccgagcagg cgacccagca gcacctcgac
       61 atcctgtccg agcttgcgca gatgctgtcg gacaagagct tccgcgagac actgctgacc
      121 gcgtcggacg cgaacgccgt ccacgtggca ctgaccacgt gggagcccgt acggcccgcc
      181 gcctgatccg accgcccttc ccgggcacgt tctcgatgcg gtagcctcgc gcgcagcccg
      241 ccggcattgt gcgggcagag gacgtccgat gccgaccttc cagtcgctct acgatgacaa
      301 ccgcgagccc ttgcggctgt cgtggatcgc cggacaagga tcggccggca aggcactgga
      361 tacgccgtca accgcgggcc ttgccagtgc cgacctcgtc ggccacctca acctgatcca
      421 cccaaatcga ttgcatgtcc tcggcgaagc cgaggtgcgc tactactcgc agatggagcc
      481 ttcgaggcgt ctgcactacg ccagcgagct gatagccggc aagccgattg gcatcgtgat
      541 cggcgagggg ctgacgccgc cgcccgaact ggtcgacacc gccgaccgcg ccggcctgcc
      601 cgccctcgcc tcacctcaat cggccgcgga gatcatcgaa gtcctgcgca tctattttgc
      661 gaaggtgctg gctgcccatt gcacgatgca tggcgtgctg atggatgtgc tcggcatggg
      721 cgtgctcgtc gccggcgaat cgggcgtcgg caagagcgaa ctcgggctcg aactcatcag
      781 ccgcgggcac ggcctggtgg ccgatgacgt ggtcgacttc tcgcgggtcg cgcccgacac
      841 gatcgagggc cgctgcccgc cactcctcgc caacctgctc gaggtgcgcg gactcggatt
      901 gctcgacatc cgttcaatct tcggagagac cgcggtccgt cggaagatga agctgcgact
      961 catcgtcgaa ttgcgtcgct atggggcgtc ggaaacgatg gagcggctgc cgctcgaggg
     1021 gcagtcgcag gacgtacttg gcattccggt ccgcaaagtg ctgatcccgg tcgcatcggg
     1081 ccgcaacctc gccgtgctga cggaagcggc ggtgcgcaat accatcctgc agctgcgtgg
     1141 catcgatacc atgcgcgact tcctgcagcg acaccagcaa cacatgaacg ggaactagca
     1201 accgcgcgga ccggtcatcg tacttctggc tgaggcccgg cgcacgtacc gtcccacttt
     1261 ttgcatgcgc ggacgccgga cacgcatgca acaattgaac ttcactactc ccgccgctgg
     1321 atgcgcatcg ttctagtcac tggaatgtcg ggctcgggca agtcggttgc catccgactg
     1381 ctcgaagacg cgggctatta ctgcatcgac aatctgccgt cgcggttcct gcgcgatgtc
     1441 tgcgccttcc tcgacagcac cggccacggc gacgtggcgc tctcggtcga ttcgcgcagc
     1501 gaagcctcgc tgggcgacat cccctccatc atcgcctcgc tgcgcggcgc cggccacgat
     1561 gtccgggtgc tgttcctgac tgcatccaat gccgcgctgg tccagcgctt ttccgaaacg
     1621 cggcgtcgcc accccatgtc aatacgtgtg cagccgcagg gtgcggaagc cacgctaaca
     1681 gagtccatcg aactcgagcg cgagctgctg gcgccgctgg tcgaggtggg tcacgtgatc
     1741 gacaccagcg aactgcaccc gaacacgctg cggcagtggg tgcggcagtt caccgacctt
     1801 ccgccggcgg cgctggtgct tgccttcgag tcctttgcct tcaagcaggg agtgcctgtc
     1861 gccgcggact tcgtgttcga cgtgcgcaat ctgccgaatc cgcattacga cccgcgcctg
     1921 cggccgctga ccggccgcga tgccgcggtc gttgcctacc tggagcggat cccgcaggcg
     1981 ggcgaaatgc tcgacgacat cgcgggcttc atcggcaagt ggctgccgag ctacgtcgcg
     2041 gacaaccggc actacctgac cgtcgccatc ggctgcaccg gcggccgcca tcgctccgtg
     2101 tatttcgtcg aggaactggc ccggcggttc cgtgcacagc gcaacgtgct ggtacgtcac
     2161 cgcgctttcg aggactccga gaccggcacg tgaactgatg cgcctgccgt tgtttccgct
     2221 gtcgaccgtg ctgttccccg ggggagttct gccgctgcgc atctttgaag cccgttacat
     2281 ggacatggca cgcgactgcc tgcgagacga gctgcaattc ggtgtctgca tgatcaccga
     2341 tcccaaggcg aatgaggcaa gtcgcaaccc cagtgtcgcc gacatcggct gcagcgcctc
     2401 catcgccgga tgggacatgc ggcagttggg cctgctacat gtacgtgccg tcggcggcca
     2461 gcgcttccgc gtgatcagtt ccaaaaccca aggcaacgga ctgaaagtgg ccgagatcga
     2521 gtgggtcgag gacgacgtcg atgcgccgat cgcgtccgat cacgttccct gtgtcgatct
     2581 gctgcgcaga atcatcgacg atctgcgtgc tcaggccgcc gaacgccggc gcgagtcgga
     2641 agacgacgat gtgcttgccc agccgccctt cgaggagccg tttcggatgg aatcgagcac
     2701 gtgggtgtcg aaccggctat gtgaggtgct accggtgccg cttaaagcca agcagaagct
     2761 aatggaactg ggcgacggcc gcgcacgcct tgagatcgtg cacacgtacc tgaagcagca
     2821 ctcggtgttc aagtagttcg agcgtcctcg acgcggtccg ccagcgtgcg caggagtcgg
     2881 cgcagtggcg ccggcagcgc cgcatgatcg acctcgtcgg cgccgagcca ctccagtccg
     2941 gccgcttgag ccacgccaat tgcgcccgtc gcgctcacca cgcgcgggtg catcagaaag
     3001 ctgtagtggc tgaactcgtg ccgcaccgac tccaggcgct gctcgaccga gccgcgcaac
     3061 ccgaaccgct tcgcaatcgc cgcctgcaac gcggcatccg aaagttccgc gtcgaactcc
     3121 ggcgcactca gcaggccccc ccagatgccg gtgggagcgc gccgctcgag caggatcgcg
     3181 ccggtgctgt cggtgatcag tgcgagcgtc gcggcacgca acggtcgcac cctcgatggc
     3241 cgcggcgcgg gtagttcggc gatgcgttca tcgcgcagcg cgatgcaggt cgagcgaacc
     3301 ggacaggcat cacagcgggg ccgcgcccgc acgcagatcg tcgcgcccag gtccatcaat
     3361 ccctgcgtat aggtttcgat gtcggcgcgt ggcaactggg ccgtgacaag ctcccacagg
     3421 acgcgctcgg ttgcgaccgc gccgggatag ccgccgactc cgaagtgcct tgcgaagacc
     3481 cgcttgacgt tgccatcgag gatcgcggcg cgttcgccga acgcaaacac cgcaatcgcc
     3541 gcggccgtgg agcgtccgat gcccggcaac tgttcaagca tcgctgccga ggagggaaag
     3601 gcgccatcgt gttcgccaac gatggtctgc gcgcaacggt gaaggttgcg cgcacgcgag
     3661 tagtaaccga gcccggccca cgccgacatc acgtcatcga tcggcgcaac cgccagcgcg
     3721 tgcacgtccg ggaatcgcgc gaggaagcgc aggtagtaag gaacgacggt cgcgacctgg
     3781 gtctgctgca gcatgatctc cgacagccag atgcggtacg catcgcgcgt gccctgccag
     3841 ggcagcccgt gccgtccatg gcgtcgctgc cacgcgacga ggagatcgga gaacttcatt
     3901 cgtggaggat aaccgccagc acgagggacg cagcgctgcg cgtctgcact ggggtcctcg
     3961 gcgatcggtc ggagctgaaa tcgacgttac gcggcgagag gaacttccgc caaccccagt
     4021 tcgtccggaa cccgcgctcc gacttcgtgc acgcgatcgg cgaatcgcct gagggcggcc
     4081 tggcgcgcct gcgtgtcctc gagcgcgagc tcgagttcgc tgatgcgttc atcgagtgat
     4141 ccctgcgcat cgcggatctt ggaaatcgat tcggcacgct tcttcagttg cgcgcgttgc
     4201 tcgcggacct gcagctcgat cggaggcaac agcgacttga cccaggcctc ggtatcccgt
     4261 tcggccgcgg aaaagatctc gcgcgacttc gccacaacgg tctcgaagaa gcgctccatc
     4321 agcgtccagc gatcgcgcgt gagcacggca aacgcaccga actgcgaacg gtaggccgcc
     4381 tcagtgcgct ccaggtcggc gatgtaggta tcgagcgaga acgtcatcgg cgggctcagg
     4441 ctccagccca gttcctcggc gaagcggcgc tgcacgccca gcaccatctg ctcgatctcg
     4501 agcgcctttg cattggcatc acgcagcaac tcgcgcaggc tgccgaaata gtcgcgcacg
     4561 gcgccggaga tggacggcga cagctgcgac ttcttcatct tgttgcgtgt cgcgaggacc
     4621 tgtgcacgca gcgtgctgac gcgcagcggc acgaagagct tgtccgagag tttctgcagc
     4681 accacgcgtg ccgcgacgat cttgcgcacc acttcctcga actcgcgctg ctcggccagc
     4741 gcccgtcgcg tcatgcg
//
LOCUS       DATIRT010000414         3537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_365784, whole genome shotgun sequence.
ACCESSION   DATIRT010000414 DATIRT010000000
VERSION     DATIRT010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365784"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VLN25_09325"
     CDS             <1..528
                     /locus_tag="VLN25_09325"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSQ10802.1"
                     /translation="AAELQAFYEANPEYYQITHGHAAPAPDAALQGFDFRPPADMSYT
                     DLPMWLIRERDSGRVVGEVSVATDLLAAGVTHLGFFMVATSRHGTGFAADVYASYEAL
                     AIGQGARWLRLGVVEGHRRAHRFWLRQGYVEVCQRHDYVLGNLSHTLRVMAKPLAANT
                     LDAYLAAVPRDRPVG"
     gene            complement(686..1291)
                     /gene="pth"
                     /locus_tag="VLN25_09330"
     CDS             complement(686..1291)
                     /gene="pth"
                     /locus_tag="VLN25_09330"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HSQ10803.1"
                     /translation="MADDTEPRNGIRLIVGLGNFGAEYAATRHNAGYWLVDAIARRAG
                     VRFSGERKFHGDAARIRLAGRDVWLLKPTTYMNRSGQSVVAVALFYKILPTEILVAHD
                     ELDLPPGAVKLKRGGGTAGHNGLKDTQALLSTADFWRLRIGIGHPRSLQLEQDVADFV
                     LHPPRRDELAAIDEAVGRGEGIVELLAEGRFEAAMLRLHTK"
     gene            complement(1399..2097)
                     /locus_tag="VLN25_09335"
     CDS             complement(1399..2097)
                     /locus_tag="VLN25_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HSQ10804.1"
                     /translation="MKVVATKRDAQGTSASRRLRHTGKVPGVLYGGSGAAQAIEVEHN
                     PLFHALRKEKFHASILDMELDGTSERVLLRDFQMHPYKPVVLHIDFQRVSENQKIHMR
                     VPLHFVNQEESPAFKLGGGLISHILSDVDVACLPKDLPEFIEVDLSGLQAYQTVRVLD
                     IKMPAGVTPVLKGKENPVVVAVSTHGEQAEEEVVAPTVAADVPATAQKAPVAAPGAAP
                     AKGGKEEKGGDKKK"
     gene            complement(2175..3125)
                     /locus_tag="VLN25_09340"
     CDS             complement(2175..3125)
                     /locus_tag="VLN25_09340"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008110854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HSQ10805.1"
                     /translation="MVPDNLMVFTGNANPKLAQAVAQQINIPLGKAQVSRFSDGEVTV
                     ELFENVRGKDVFILQPTCAPTNDNLMEIMIMADALKRASAGRITAAIPYFGYARQDRR
                     PRSARVAISAKVVANMLQVVGINRVLVMDLHADQIQGFFDIPVDNIYASPILLGDLWK
                     HDYDRLVVVSPDVGGVVRARALAKRMEADLAIIDKRRPRANVAEVMHIIGEVEGRNCV
                     IMDDMVDTANTLCQAAQALHDSGAQRVMAYATHPVLSGKAVERLMNSVIDELVVTDTI
                     PLAPAAAACPKIRQLSCAALLGETISRIAREDSVSSLFIE"
     gene            complement(3284..3360)
                     /locus_tag="VLN25_09345"
     tRNA            complement(3284..3360)
                     /locus_tag="VLN25_09345"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3324..3326),aa:Gln,seq:ttg)
BASE COUNT          589 a         1162 c         1133 g          653 t
ORIGIN      
        1 gccgccgaac tccaagcgtt ctacgaggcg aatccagagt attaccaaat cacgcacggg
       61 catgctgcgc ccgcgcccga tgccgcgttg cagggattcg acttcaggcc gcccgccgac
      121 atgtcgtaca ccgatttgcc gatgtggctg atccgcgagc gtgactccgg tcgggtggtc
      181 ggcgaggtct cggtggctac cgacctgctg gcggcgggtg tcacccacct cggcttcttc
      241 atggttgcaa cgtcgcggca cggcacgggt ttcgcagccg acgtttatgc gtcctatgaa
      301 gccttggcaa tcggtcaagg cgcgcgttgg ctgcgcctcg gcgtggtcga aggacatcga
      361 cgcgcccacc gcttctggct gcgacagggc tacgtcgaag tgtgccaacg ccacgactat
      421 gtgttgggca acctcagcca cacgctgcgg gtcatggcga agccgctggc tgcaaacacg
      481 ctggacgcgt acctcgccgc ggtgccgcgc gaccgccctg tcggctaaag caaccgatgt
      541 ccgacggcga caggcctcga tcagcgaggt gctgccgggc acgctcgccg acgcggacat
      601 cgatccagtg ttcggtcatg aaggagtctg attacctacg atgcggccgg ccgcgtgacc
      661 gggtcgcgag aattgcgccg cgacgctact tcgtatgcaa ccgcagcatg gccgcctcga
      721 agcggccttc ggccaggagt tcgacgatgc cttcgccgcg acccacggcc tcgtcgatcg
      781 cggccagttc atctcggcgc ggcggatgca ggacgaagtc ggccacgtcc tgctcgagct
      841 gcaacgaacg ggggtggccg atgccgatgc gcagccgcca gaagtccgcg gtgctgagga
      901 gtgcctgcgt gtccttgagt ccgttgtgac cggccgtacc gccgccacgc ttgagtttga
      961 cagctcccgg cggcaggtcg agttcgtcgt gcgctaccag gatctcggtc ggcagaatct
     1021 tgtagaagag ggcgacggca acgacggact ggcctgatcg gttcatgtac gtcgtcggtt
     1081 tcagcagcca gacgtcgcgg cccgcgagtc ggatgcgcgc ggcatcacca tggaacttgc
     1141 gctcgcccga aaagcgcacg ccggcccggc gtgcgatcgc gtcgaccagc caatagccgg
     1201 cgttgtggcg cgtcgccgcg tactcggcgc cgaagttgcc gagcccgacg atcaggcgga
     1261 tgccgttcct gggttcggtg tcgtcagcca tggatacaaa gaaaaaggcc cgccgtcacg
     1321 aggacagcgg gccgcattgt gccggactgc ggccgtcggg ccacgaggcc cgggcagcgc
     1381 gcgccgatct tctttggctt acttcttctt gtctccgcct ttttcttcct tgccaccctt
     1441 ggccggcgca gcaccgggtg cggccaccgg agccttctgt gcggtcgccg gaacgtccgc
     1501 tgccaccgtc ggcgcaacga cttcttcctc agcctgctcg ccgtgcgtcg acaccgccac
     1561 cacgaccgga ttttccttgc ccttcagcac cggggtcacg cccgccggca tcttgatgtc
     1621 cagcacgcgc actgtctggt acgcctgcag gccggacaga tcgacctcga tgaactcggg
     1681 caggtccttc ggcagacagg cgacatcgac gtcggacaag atatggctga tcaggccgcc
     1741 accgagcttg aacgccgggg actcttcctg gttgacgaag tgcagcggca cgcgcatatg
     1801 gatcttctgg ttctcggaca cgcgctggaa gtcgatatgc aggacgaccg gcttgtacgg
     1861 gtgcatctgg aagtcacgca gcagcacgcg ctcggaggtg ccgtcgagtt ccatgtcgag
     1921 gatcgacgca tggaactttt ccttgcgcag ggcgtggaag agcgggttgt gctcgacctc
     1981 gatcgcctgc gcggcaccgc tgccgccgta aaggacgccc gggaccttgc cggtgtggcg
     2041 caggcggcgg ctcgcactcg tgccttgcgc gtctcgtttg gttgctacga ctttcatttc
     2101 acactcctgt tgtcgtccgc ggaccgcgac cagtcacgac gaacggttat ggcgcggcga
     2161 catgccgcac cgaattactc gatgaaaagt gagctcacgg aatcctcacg cgcaatacgc
     2221 gaaatcgttt cgcccagcaa tgccgcgcaa gacaactgcc ggatcttggg acacgccgcc
     2281 gccgccgggg ccaggggaat cgtgtcggtc acgaccagtt cgtcgatgac cgagttcatc
     2341 agtcgctcga ccgccttgcc cgacagcacc gggtgcgtgg catacgccat cacacgctgc
     2401 gcgccgctgt cgtgcaaggc ctgcgcggcc tgacacagcg tgtttgcggt gtcgaccatg
     2461 tcgtccatga tcacgcagtt gcgcccctcg acctcgccga tgatgtgcat cacctcagcc
     2521 acgttggcgc gcggacggcg cttgtcgatg atcgcgaggt ctgcttccat tcgtttggcg
     2581 agtgcgcgcg cgcgcaccac gccgccgacg tccggcgaca caacgaccag gcggtcgtag
     2641 tcgtgcttcc acagatcgcc gagcaggatc gggctggcgt agatgttgtc gaccgggatg
     2701 tcgaagaaac cctggatctg gtcggcgtgc aggtccatga ccagcacccg gttgatgccg
     2761 accacctgca gcatgttggc gacgaccttg gcgctgatgg cgacgcgcgc actgcgcggc
     2821 cgccgatcct ggcgggcgta gccgaaatac ggaatcgctg cagtgatccg gccggcggag
     2881 gcgcgcttaa gcgcgtcggc catgatcatg atttccatca ggttgtcgtt ggtcggcgcg
     2941 caggtgggct gcaggatgaa cacgtctttg ccgcgcacgt tctcgaacag ttcgaccgtc
     3001 acctcgccat ccgagaaacg cgagacctga gccttgccga gcggaatgtt gatctgctgt
     3061 gccacggcct gcgcgagctt cgggttcgcg ttgcccgtga acaccatcag gttgtcgggg
     3121 accatcaagg ccatggctgg cacgcctgcg cggcattcaa tgtgaatggg ctgtcgcccg
     3181 ggcgccggaa ccgagccgca tcgaggcggc gccccataaa gaggctcgcc gtgatgcatt
     3241 cgctacgcac tggcgtcgcg tacttcacgg cggccctacg atttggctgg ggaggaagga
     3301 ttcgaaccct cgaatggcgg aatcaaaatc cgctgcctta accagcttgg cgactcccca
     3361 gcacctgaaa ctgtgctgca ctgctgttgc agatcccact tccgttctgc ccggttcggt
     3421 gaatcgaacc gcacttttgc tctctaccac gcgcgcagcg gatgctcctg caatccgcaa
     3481 accgcccagc cctgccaacc cggcggcaga tgggcgatcg ctgcctgcgc cgcctct
//
LOCUS       DATIRT010000415         2600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_561904, whole genome shotgun sequence.
ACCESSION   DATIRT010000415 DATIRT010000000
VERSION     DATIRT010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561904"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1148)
                     /locus_tag="VLN25_09350"
     CDS             complement(<1..1148)
                     /locus_tag="VLN25_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HSQ10806.1"
                     /translation="MNAPLPEAVRRALAEVTLDDKWTLDRGRAYMSGTQALIRLAMLQ
                     RQRDQLAGLNTAGFISGYRGSPLGGIDQTAWKAKKHLERHHVKFQPGLNEDLAATSVW
                     GTQQVNMFEGAKYDGVFAMWYGKGPGVDRCGDVFKHANAAGTSRHGGVLALAGDDHGA
                     KSSTLPHQSDHIFKACMMPVLFPASVQEYLDMGLHGFAMSRYAGVWVGMKTVTEVVEA
                     SASVIVDPERVDIQLPVDFAMPPGGLNVRWPDGALEMEARLLDYKLYAALAYCRANKL
                     NHTVIDSPHARFGIVASGKAYLDTRQALADLGLDEQTCKDIGIRLFKCGMVWPLEATA
                     IRGFAEGLEEILVVEEKRQFVEYQLKEELFSWIGTGKRIPRVIGKFDE"
     gene            1393..>2600
                     /locus_tag="VLN25_09355"
     CDS             1393..>2600
                     /locus_tag="VLN25_09355"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase N-terminal
                     domain-containing protein"
                     /protein_id="HSQ10807.1"
                     /translation="MLRLLIIAAVFVPLVVAATTLDLKRLGAPAEACTDFDEYVNGLW
                     RKATPIPPDRARIGSFDTLRDESRDIVEQALIDAARDPSQLDTPGKLLAFRFYISGMD
                     VEAIEKRRLESLQPLLRQIDALSDRKQLPALLAQLSRHFIDAPLRAAVMPDAKDRRRY
                     IVQLDQGGLGLPDRDDYFRDDARTLELRKAYEVYRLRLGQLAQAGDAPAAAVASFELQ
                     KSLADASLTRVERRDPNALYQLNTLETVMQRAPGLDWTAYFAALGVAKPGEFNVAAPK
                     FVAAVGRAAAEAPLATWRAYLRERVLDEISPMLPAEFVDARFDYRGTVVQGLEKNFPR
                     PDQVIRAMTGPFGSEPLAEGLGQLYVARAFTPEAKARAVQMITDIKSALRARIEKLDW
                     MSAPTKQSAL"
BASE COUNT          429 a          860 c          851 g          460 t
ORIGIN      
        1 ttttcgtcga acttgccgat cacgcgcgga atgcgcttgc cggtgccgat ccacgagaag
       61 agctcctcct tcagctggta ctcgacgaac tggcgctttt cctcgaccac gaggatttcc
      121 tccagccctt cggcaaagcc ccggatcgcg gtcgcctcga gcggccacac catgccgcac
      181 ttgaagagcc gtatgccgat gtccttgcag gtctgttcgt cgagacccag gtccgccagc
      241 gcctggcgcg tatccaggta ggccttgccg ctggcgacga tgccgaagcg cgcgtgcggt
      301 gaatcgatca ccgtgtggtt gagcttgttc gcgcggcaat acgccagcgc cgcatacagc
      361 ttgtagtcga gcaggcgcgc ttccatctcg agcgcaccgt ccggccagcg gacgttgagg
      421 ccgccgggcg gcatcgcgaa atcgaccggc aactggatat ccacgcgctc cggatcgacg
      481 atcaccgagg cggacgcctc cacaacttcg gtcaccgttt tcatgccgac ccacacgccc
      541 gcgtaacggc tcattgcgaa accgtgcaga cccatgtcga ggtactcctg cacggaggcc
      601 ggaaacagca ccggcatcat gcaggccttg aagatgtggt cggactggtg cggcagcgtc
      661 gacgacttgg ccccatggtc atcgccggcc agcgccagca cgccgccgtg ccggctggtg
      721 cccgccgcat tggcgtgctt gaacacatcg ccgcagcgat ccactccggg gcctttgccg
      781 taccacatgg caaatacgcc gtcgtacttc gcgccctcga acatgttgac ctgctgcgtg
      841 ccccagacgc tggtggcggc gaggtcctcg ttcagccccg gctggaattt cacgtggtga
      901 cgttcgagat gtttcttcgc tttccacgcc gtctggtcga tgccgccgag cggcgagccg
      961 cgatagccgc tgatgaaccc ggcagtgttc agccccgcca actggtcgcg ctgccgctgc
     1021 agcatcgcga gccggatcag tgcctgcgtg ccgctcatgt acgcgcggcc gcggtccagg
     1081 gtccacttgt cgtcgagggt cacctccgcc agtgcacggc ggacggcttc gggaaggggc
     1141 gcgttcatca ggactcctgc caaaaatcgt gttcttggga gcgcggcagt cgggtgggtg
     1201 cccctgtgcc gtgcagccgg agcgtgcctt gtgagcgcgc tcggcgaggt tccgcggtga
     1261 gcggaatttg aaagtggcgg cgagcgtagc accgacgtag cgcccgctca atcatgcatt
     1321 gcgggtgggg tcacgcccgg ccgggtgcaa tgcgcgaggt atgctgccgg ccggtttacg
     1381 accggatagt ccatgctgcg gctactgata attgctgccg ttttcgtgcc cctggtcgtc
     1441 gccgccacca cgcttgatct caagcgactc ggtgcgccgg ccgaggcctg tacggacttc
     1501 gacgagtacg tcaacgggct ctggaggaag gcgaccccga tcccgcctga ccgggcacgc
     1561 atcggaagtt tcgataccct gcgagacgaa agccgggaca tcgttgaaca ggccttgatc
     1621 gacgccgcac gcgatccttc ccagctcgac acgccgggca agctcctcgc gttccggttt
     1681 tatatcagcg gcatggacgt ggaggccatc gaaaagcgcc ggctcgaatc gctgcagccg
     1741 ctgctgcgac agatcgatgc gctcagtgac cgcaagcagc tgccggcgtt gctggcccag
     1801 ttgtcgcgtc acttcattga cgcgccactg cgggccgcgg tgatgcccga tgccaaggac
     1861 cgccggcgct acatcgtcca gcttgaccag ggcggcctgg gcctgcccga tcgcgacgac
     1921 tacttccgcg atgacgcgcg cacgctcgaa ctgcgcaagg cgtacgaggt gtaccgcctg
     1981 cgtctcggac agctcgctca ggcaggcgat gcgcccgcgg cggcggtggc gagcttcgaa
     2041 cttcagaaat cacttgccga tgcttcgctg acgcgggtcg aacggcgcga cccgaacgcg
     2101 ctttaccagc tgaacacgct cgaaacggtg atgcagcgcg cgccgggtct ggactggacg
     2161 gcctactttg ctgcgctggg cgtggcgaag cctggcgagt tcaacgtcgc cgcgccgaag
     2221 ttcgtcgccg cggtgggccg cgccgcggcc gaagcgccgc tggcgacctg gcgcgcgtac
     2281 ctgcgcgaac gcgtcctcga tgaaatttcg ccgatgctcc cggccgaatt cgtcgacgcg
     2341 cgcttcgact atcgcggcac ggtggttcag ggcctggaga aaaacttccc gcgcccggat
     2401 caggtcatcc gagcaatgac gggtccgttc ggttcggagc cgttggcgga gggactcggg
     2461 cagctttatg tcgcgcgcgc gtttacgccg gaagccaagg cgcgcgcggt gcagatgatc
     2521 accgacatca agtcggcgct gcgggcgcgg atcgagaagc tcgactggat gagcgcgccg
     2581 accaaacaga gcgcgctgaa
//
LOCUS       DATIRT010000416         3766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1448161, whole genome shotgun sequence.
ACCESSION   DATIRT010000416 DATIRT010000000
VERSION     DATIRT010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448161"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..785
                     /locus_tag="VLN25_09360"
     CDS             177..785
                     /locus_tag="VLN25_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSQ10808.1"
                     /translation="MAPLLEVDRLSKVYRRRHFGKPTFELNATLRFDAPAIIGVLGPN
                     GSGKTTLFELITGLNQPSDGEVRVRGQAIHRVKRNQRAQLARHYHQSYQMRRFHEWTP
                     EVLQQRAVLSEAVVHLFDEPQLNTQDGYIGFMLSFFRRLRGQGKLIFVCLHPNERWQL
                     DLLRELAESLVFVHRGVVTEYADFASLAARLEAREYLGTLLD"
     gene            complement(863..1387)
                     /locus_tag="VLN25_09365"
     CDS             complement(863..1387)
                     /locus_tag="VLN25_09365"
                     /inference="COORDINATES: protein motif:HMM:NF022214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 family protein"
                     /protein_id="HSQ10809.1"
                     /translation="MHELSTPSYPKTHSRSGPQSDLEFKGVRGWLLALCLMLTVIGPA
                     IAVWLMVIEYTSAEPLFRESPGRQAAALGSLLLSGCSVVFGVYAGVRLWLVLPNAVRT
                     AKYALLFGLAVDIVTTAFEAAAAQVPSHQLLFQVEIGLIPTLIFFTVCFTYLNQSKRV
                     HATYGHPQPVRKLS"
     gene            1732..2142
                     /locus_tag="VLN25_09370"
     CDS             1732..2142
                     /locus_tag="VLN25_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HSQ10810.1"
                     /translation="MAGQVIRREVESEKITINLGYVDLGQIDLLVQEGFYSNRTDFIR
                     TAIRNQLNNHGDVVKQTVARRSMVLGLQHFSREDLEHVRKAGEQLHIQVLGLASINDD
                     VSPKLAAQTIASIAVLGALRASPAVRAALADRIR"
     gene            2176..3333
                     /locus_tag="VLN25_09375"
     CDS             2176..3333
                     /locus_tag="VLN25_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HSQ10811.1"
                     /translation="MDKGILAGIAQALQLTKAGRLGEATRLIQESLARRRQPDRFDEP
                     APQNGDAVDDIVEGQWAEVTPPTVAVSAIETVQAELRPEPRADDAPPRTAPPVTRQPS
                     EWIAGDFENAAGHRAYKLYVPSRSPGQSLPLIVMLHGCQQSPDDFAAGTQMNIRAEEH
                     GCLVLYPAQSNSANPSRCWNWFRREDQVREAGEPSLIADMTRDVVRRYGVDPLKVFVA
                     GLSAGGAMAAVMAVNYPDLYAAVGIHSGLASGSAHDLPSALAAMKGLSVSMGPRGNSS
                     PVHATPTIVFHGDHDRTVHPRNGIDAIANSAHWEGGQGGAVVMEQGQAPGGHAFTRTV
                     HRDAAGQLRLEHWLVHGGGHTWFGGSRAGSYSDPRGPDASAEMMRFFLQRT"
     gene            complement(3371..3446)
                     /locus_tag="VLN25_09380"
     tRNA            complement(3371..3446)
                     /locus_tag="VLN25_09380"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3411..3413),aa:His,seq:gtg)
     gene            complement(3460..3536)
                     /locus_tag="VLN25_09385"
     tRNA            complement(3460..3536)
                     /locus_tag="VLN25_09385"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3500..3502),aa:Arg,seq:tct)
     gene            complement(3571..3647)
                     /locus_tag="VLN25_09390"
     tRNA            complement(3571..3647)
                     /locus_tag="VLN25_09390"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3611..3613),aa:Pro,seq:tgg)
BASE COUNT          744 a         1170 c         1189 g          663 t
ORIGIN      
        1 cgtgcgcatg aagtccgaag gactcggcat cctgctggtc gagcagaatg tgcacgcggc
       61 gctttcggtg gccgaccgcg tgatcgtgct tgatcacggc gccgtagcgt atcgcgggtc
      121 cgcggccgaa ctgcgcgacg acgcggccct gcggcagcgc ctgctggggg cctagcatgg
      181 cgccgttact tgaagtcgat cgcctcagca aggtgtaccg gcgccgccac ttcggtaagc
      241 cgactttcga actcaacgca acgttgcgat tcgacgcacc agccatcatt ggcgtgctgg
      301 gtccaaacgg ttcaggcaag accacgctgt tcgagctgat cactggcctg aaccagccga
      361 gcgatggcga ggtgcgagtg cgcggccagg cgatccaccg cgtgaagcgt aaccagcgag
      421 cacaactggc gcggcactat caccagtcgt accagatgcg acgcttccac gagtggactc
      481 ccgaagtcct gcagcagcgg gctgttctct ccgaagccgt ggtacatctc tttgacgagc
      541 cgcagctcaa cactcaggac ggctacatcg gtttcatgct gagcttcttc cgtcgccttc
      601 gcggccaggg caagctgata ttcgtatgcc tgcatccgaa cgaacgctgg cagctcgacc
      661 tgctgcgcga gctggcggag agtctggtgt tcgtgcaccg cggcgttgtg acggagtatg
      721 cggacttcgc atcactggct gcacgactcg aggcacgcga ataccttggg acactgctgg
      781 attgagctcg atttgagctc gacccggcgg gtcaatttgt tagcgcttca atgatgtcaa
      841 gcaacgctcc gacgctccgg tgtcaggaaa gcttacgcac cggctgaggg tggccgtagg
      901 tggcgtggac gcgcttggat tgattgaggt atgtaaagca cactgtgaaa aagatgagcg
      961 ttggtatcag gccaatttca acttgaaaga gcagctgatg actgggtacc tgcgcagctg
     1021 cggcttcaaa cgccgtggtc actatgtcga ctgccagccc gaagagaagc gcgtacttcg
     1081 ctgttctaac cgcgttcgga aggacgagcc acagacgcac gcctgcatat acgccgaaga
     1141 caaccgagca tccagaaagc aacagtgaac caagcgccgc cgcctgccta cccggagatt
     1201 cgcggaacag aggctctgcg cttgtgtact cgatcaccat cagccagaca gcgatggccg
     1261 ggccgatcac tgtcagcatc aggcaaaggg cgagaagcca accgcgaacg cccttgaact
     1321 ccagatcgga ttgagggccc gaccgcgagt gcgtcttagg gtaactcggt gtggacagct
     1381 catgcatcag acttcctccc agtgggcaga gcgggacagc tgcgcttccc ggctgcgcaa
     1441 acccattttg cccttggatt tgccggggcg gcaggccatt cgacgaactg ctggtcgaac
     1501 tgggtgaagt gcagatagtt gcgacgggct gcaggggacc gttgcatcga gtcgtgcgga
     1561 gcggcgaagc attgcgtcat tcagcggaca aatccgccgc tggagacgac gcgtcgggcg
     1621 gggaaacttt ccgcgtccgg cctcccctgt tgtgagtccc tgaggcgcag aatccgggct
     1681 atactccatc cggacagatg gtaaggagat ggtgcaggga tggtgtcaaa gatggcaggt
     1741 caggtcatac gcagggaagt cgagtcggaa aagatcacga tcaatctcgg ctacgtcgat
     1801 cttgggcaaa tcgacttgct ggtgcaggag ggtttctact cgaatcgcac cgacttcatc
     1861 cgcacggcga tccgcaacca gttgaacaac cacggcgacg tcgtgaagca aaccgtcgcc
     1921 cggcgttcga tggtcctggg actgcaacac ttttcccgcg aggatctcga gcatgtccgt
     1981 aaggccggcg agcaactgca catccaggtg cttggtctgg catcgatcaa cgacgacgtt
     2041 tcgcccaaac tcgcggcgca gaccatcgct tcaattgcgg tgctcggtgc actgcgcgcc
     2101 agcccggccg tgcgtgccgc actggccgat cgcatccgct gaccgcggcg ggcgatcctt
     2161 tacaggaaca cagcaatgga caaaggcata ctggccggaa tcgctcaggc acttcagctg
     2221 acgaaggccg ggcggctcgg cgaagctacc cggctgatcc aggagagtct ggcgcgccgt
     2281 cgacaaccgg atcgcttcga cgagcctgca ccgcaaaacg gtgacgccgt cgatgacatc
     2341 gtcgagggac agtgggcgga ggtcacgccg ccgaccgtcg ccgtatcggc catcgaaacg
     2401 gtccaggcag aactgcgacc agaaccccgc gcggacgacg ccccgccccg caccgctccg
     2461 cccgtcacga ggcagcccag cgaatggatt gcgggtgact tcgagaacgc ggccggacac
     2521 cgtgcctaca aactttacgt gccgtcccgg tcccccggac agtcgttgcc gctgatcgtc
     2581 atgctgcacg gttgccagca atcgccggat gacttcgctg ccggcacgca gatgaatatc
     2641 cgagccgagg agcacggctg cctcgtcctt tacccggcac agtcgaactc cgccaatccg
     2701 tcccgctgct ggaactggtt tcgccgcgag gatcaggttc gcgaggccgg ggaaccttcc
     2761 ctcatcgccg acatgacccg ggacgtcgtc cgtcgatatg gcgtggatcc cctcaaggtc
     2821 ttcgtcgcag gcttgtcggc aggtggcgcg atggccgcgg tgatggcagt gaactaccct
     2881 gacctgtacg cggcagtggg gatccactcg ggtctcgcgt ccggcagtgc gcacgatctg
     2941 ccgtccgcac tggcggccat gaagggtctg tccgtttcaa tgggcccgcg ggggaactct
     3001 tcaccggtgc acgcaacccc aaccatcgtc ttccacggcg atcacgaccg gaccgttcat
     3061 ccgcgtaacg gcatcgacgc aattgccaac tccgcgcatt gggaaggcgg tcaaggtggt
     3121 gctgtggtga tggagcaggg ccaggcgccg ggcggtcacg cctttacgcg gaccgtccac
     3181 cgagacgcgg ctgggcagct gcggcttgaa cactggcttg tgcatggagg cggacacacc
     3241 tggtttggcg gcagtcgcgc cggctcgtac agcgacccgc ggggcccgga cgcatcggct
     3301 gaaatgatgc ggttctttct tcagcgaact tgaccacgtt gcattggctt cgcctggctc
     3361 cgcagaaaga tggggtggct gacgggactc gaacccgcga caaccggaat cacaatccgg
     3421 gactctacca actgagctac agccaccact gaagaaaact ggcctgcccg acaggaatcg
     3481 aacctgtgac cctcagctta gaaggctgat gctctatccg actgagctac gggcagatcg
     3541 aaactcgccg ggtgcgcccc ggagggcgcg tggtcggggt ggagagattc gaactcccga
     3601 catcctgctc ccaaagcagg cgcgctacca gactgcgcta taccccgccg gcagaaccga
     3661 ccgcatcgct gcgtttgcag gctcccgcga cgcaccagcg tgacgaaagg ccgggcattg
     3721 tccggccgcg cggccgggct gtcaacgcgg cgctcgcaaa aggtga
//
LOCUS       DATIRT010000417         1523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_661336, whole genome shotgun sequence.
ACCESSION   DATIRT010000417 DATIRT010000000
VERSION     DATIRT010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661336"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            284..844
                     /locus_tag="VLN25_09395"
     CDS             284..844
                     /locus_tag="VLN25_09395"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSQ10812.1"
                     /translation="MDRRTACATLMALLLTAAATSTEAVETEVRISRDGELVLIDAVL
                     LAPVTPAEAWAVLTDFDAMARYVPDLDASRVTIRLGNRLRVEQRGVARWGPFARPFTM
                     VREVDLTPVARVESRSIGGTLERVRTSTRLVPVADGTEVRHHLEFVFNVWMPDLLADA
                     FLRYKVREQFDAVVEEMLQRRISGRK"
     gene            complement(861..>1523)
                     /locus_tag="VLN25_09400"
     CDS             complement(861..>1523)
                     /locus_tag="VLN25_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008543496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSQ10813.1"
                     /translation="GSRKHVMSYLGEFLFSPQRANSPIKTLSGGERNRLLLARLFARP
                     ANLLVLDEPTNDLDIESLELLESTLQGYNGTLLLVSHDRTFLDNVVTQSLVAEGDGRW
                     TEYPGGYSDWLALRPKSAPAPIATLVDPPHGARTAARGGRLKLSFREQRELEALPAKI
                     EALEREQHEITARMNSVEYHRIGVDAIRADRLRATAIEHELAACFERWSLLEDKARAA
                     SG"
BASE COUNT          243 a          493 c          520 g          267 t
ORIGIN      
        1 gtttccctgg cccggtgtgt cgccgggacg cacttcctgc gcatcgtgcg cggcgtgatg
       61 ctcaagggag cggggctcgc cgacgtcgca ccgcaaacgg tgccgatcct gatcttcatc
      121 gccgttgccg ccgccgtcgc gatgaagcgc tacaaacaga cactcgactg acgggcagca
      181 gcgtcccgcg gcggcaaatc aatcggcaat cgtaggtctg gaagcgaggg cgggtcccgt
      241 ccatggccct gcgacgcgct acgctgccgg catggatcga cgcatggatc gacgaactgc
      301 gtgcgccacc ttgatggcct tgctgctgac cgcagccgca acatcgacgg aggcggtcga
      361 gaccgaggtt cggatttccc gcgacggcga actcgtcctg atcgacgccg tgctgcttgc
      421 gccggtgacg ccggccgagg catgggcggt gctcactgac ttcgacgcga tggcgcggta
      481 cgtcccggac ctggacgcga gccgggtgac cattcgcctc ggcaatcgcc tccgggtcga
      541 acagcgcggc gttgcgcgct ggggcccgtt cgcacgtcct ttcacaatgg tgcgtgaggt
      601 cgacctgacg ccggtagcgc gagtcgagtc gcgctcgatc gggggcacgc tggaacgggt
      661 tcggacatcg acccggctgg tcccggtggc agacggaacg gaggtgcgcc atcacctcga
      721 gttcgttttc aatgtctgga tgccggacct cctggccgat gctttccttc gctacaaggt
      781 gcgcgagcag ttcgacgcgg tcgtggaaga gatgctgcag cggcgtatct ccggcaggaa
      841 atagcgctgt tcacccagcg ctagccggag gcggcgcgcg ccttgtcctc gagcagtgac
      901 cagcgctcga agcacgcggc gagttcgtgt tcgatcgccg ttgcacgcag gcgatcggca
      961 cgaatcgcgt cgacgccaat gcggtgatat tcgacgctgt tcatccgcgc ggtaatctcg
     1021 tgctgctcgc gttcgagggc ctcgatcttg gccggcaggg cctcgagttc gcgctgttcc
     1081 ctgaacgaga gcttcaagcg cccgccgcgg gcggccgtcc gcgcgccgtg cggcggatcg
     1141 accaacgtcg caatcggcgc cggcgctgac ttcggacgca atgccagcca gtcgctgtag
     1201 ccaccggggt actcggtcca gcggccatcg ccttcggcaa cgagcgactg cgtgacgacg
     1261 ttgtcgagga aggtccggtc gtggctgacc agcagcagcg tgccgttgta cccctgcagc
     1321 gtcgactcca gcagttcaag cgactcgatg tcgagatcgt tggtcggctc atccagcacc
     1381 agcaggttgg ccggtcgcgc gaagaggcgc gccagcaaca ggcggttgcg ctcgccccct
     1441 gacagcgtct tgatgggtga attcgcgcgc tggggcgaga acaggaactc gccgagatag
     1501 ctcatcacgt gcttgcgcga acc
//
LOCUS       DATIRT010000418         5510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_168975, whole genome shotgun sequence.
ACCESSION   DATIRT010000418 DATIRT010000000
VERSION     DATIRT010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5510
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168975"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VLN25_09405"
     CDS             <1..622
                     /locus_tag="VLN25_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HSQ10814.1"
                     /translation="EYWSRAKRTLGRRDATMARIISAHPRVALATRGDPFTTLARSIV
                     GQQISVKAAESVWSRFSTVCGEMTPRAVLRRRAPTLRACGLSDRKVEYIRDLAQHIVS
                     GRVDPARFAGQGDEEIIAELIDIRGIGRWTAEMFLIFNLLRPNVLPLDDLGLLKAVSL
                     NYFDGAPMTGKDARAQVTALAAAWEPWRSVATWYLWRSLDPVPVEY"
     gene            646..1620
                     /locus_tag="VLN25_09410"
     CDS             646..1620
                     /locus_tag="VLN25_09410"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HSQ10815.1"
                     /translation="MAKTTFLDFEQPIAELEAKIDELRFVQDDSAVDISEEIERLQKK
                     SQGLMKDIYAKLSPWQVSQIARHPQRPYTLDYVEDIFTDFHELHGDRAFSDDLSIIGG
                     LARFNGQTVFVLGHQKGRDTKERALRNFGMSKPEGYRKALRLMKLAEKFGLPVFTFVD
                     TPGAYPGIDAEERGQSEAIGHNLYEMSQLKVPIITTIIGEGGSGGALAIAVADQVLML
                     QYATYSVISPEGCASILWKSADKAPEAAEALGLTAHRLKALGLIDKIVSEPLGGAHRD
                     SKQMSALLKRGLADALRQFTGMKPRELVAARHARLLAYGRFKNTTEET"
     gene            1586..3061
                     /gene="tilS"
                     /locus_tag="VLN25_09415"
     CDS             1586..3061
                     /gene="tilS"
                     /locus_tag="VLN25_09415"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HSQ10816.1"
                     /translation="MAASRTRPKRPDPLDELLVNAASAALRRAQARLEAIDRSGDEAL
                     RRRSARRQAITAVVGYSGGRDSTALLQVMAGLARKRGTGLSEVIAVHVHHGISRHSDA
                     WLAHCEAGATALGLRFVGRRVTVQRAGRGLEAAARDARYEALAEVAADVGAQVVCCAH
                     HRDDRVETFLIQWMRGAGPEGLAAFPPSRRFDADGLLLVRPFIDIARADIERYVDRHG
                     LDYIEDDSNDDRSLLRNALRLDVLPRIEALRPGFRAAAARSVDLVAEATEALRSVADD
                     DLALCQDAAPVGMLRLDVLATLPAARQTWVLRAWLASHGLEALSRARLVEMLEQARDA
                     RSDARLLVRIGAREIRRYRGLLLLKDSDAGAGDAEAFRWTGEDEIALPGWGGVLRFHR
                     TQDREGFEPDWLSAMPLEARPRAGGERFKPHAGRPSRTLKRLYQDAGIADFDRARLPL
                     LWRDGELIFVAGLGADVRLTDRDGERVTIEWQPDATLIGVD"
     gene            3242..4498
                     /locus_tag="VLN25_09420"
     CDS             3242..4498
                     /locus_tag="VLN25_09420"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011885194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HSQ10817.1"
                     /translation="MALIVHKYGGTSVGTVDRIKNVARRIEKWHKAGHQLVVVVSAMS
                     GETNRLLGLAKEVQSNPDPRELDVVASTGEQVTIGLLSMALKDRGLLAKSYTGPQVHV
                     LTDSAFTKARILEIDEKNIRRDLEQGAIVVVAGFQGADEYGNITTLGRGGSDTSAVAL
                     AAALKADECLIYTDVDGVYTTDPRIVPEARRLDKVTFEEMLEMASLGSKVLQIRSVEF
                     AGKYKVPLRVLSSLTEPGLPLAEEASSGTLITFEEASMMEKAVISGVAFSRDEAKITL
                     TRVPDRPGIAYQILGAIADANIDVDMIVQNISVEGTTDFSFTVHRNEYQKALDVLNTQ
                     VKDHVAAKQIVGDPKIAKVSVVGIGMRSHVGIASLMFRTLSEEGINIQMISTSEIKIS
                     VVVEDKYMELAVRALHKAFGLEQAPV"
     gene            4569..4662
                     /locus_tag="VLN25_09425"
     tRNA            4569..4662
                     /locus_tag="VLN25_09425"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4603..4605,aa:Ser,seq:gct)
     gene            4794..5453
                     /locus_tag="VLN25_09430"
     CDS             4794..5453
                     /locus_tag="VLN25_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10818.1"
                     /translation="MKMLRIILPVAALLAAHGALAAGVGVRAGTTGIGADVAVEVLPT
                     LSARVGYSYLSFSTSVDSSEVEYDAKPKLSNGNLFLDWSPLGPFRITGGLILNDNKVD
                     VTGVPTGGTFTLNGVVYPAAAIGSLSGTVKSGKSAAPYLGIGYGNVSGAGVNVYFDLG
                     VMFPGNPKATLTATCGPSLPPAQCSQLQSNAAAEAIDLQDSIKNFKYFPVASFGVTIG
                     F"
BASE COUNT          983 a         1772 c         1822 g          933 t
ORIGIN      
        1 ggagtactgg tcccgcgcca agcgcacgct ggggcggcgc gatgccacga tggcgcgcat
       61 catttctgcg catccgcgcg tggcgcttgc cacgcgcggc gatcctttca cgacactggc
      121 acgctcgatc gtcgggcagc agatctcggt gaaggcggca gaatcggtct ggagccgttt
      181 ttcgaccgtg tgtggcgaga tgacaccgcg ggccgtgctg cggcgtcggg cgccgacact
      241 gcgcgcgtgc gggctttcgg atcgcaaggt cgagtacatc cgtgacctcg ctcagcatat
      301 cgtcagcggt cgcgtcgatc cggcccgctt tgccgggcag ggcgacgaag aaatcatcgc
      361 cgaactgatt gatatccgag gcatcggccg ctggactgcc gagatgttct tgatcttcaa
      421 cctgctccgg cctaatgtcc tgccgctgga tgacctggga ctgctgaaag cggtgagtct
      481 caactatttc gacggggcgc cgatgaccgg caaggacgcg cgcgcgcagg tcaccgcact
      541 cgcggcggca tgggagccgt ggcgcagcgt ggcaacctgg tatctctggc gcagcctgga
      601 cccggttccc gtggagtact gacaggactt cgaaacagaa cccaaatggc gaaaaccaca
      661 ttcctcgact tcgagcagcc gatcgcggag cttgaagcca agatcgacga actgcgcttc
      721 gtgcaggacg actcggcggt ggacatctcc gaggagatcg agcgcctgca gaagaagagc
      781 cagggcctga tgaaggacat ctacgccaag ctctcgccct ggcaggtatc gcagatcgcg
      841 cgccatccgc agcggccgta cacgctcgac tacgtcgagg acatcttcac cgacttccac
      901 gaactgcacg gcgaccgcgc cttttcggac gatctgtcga tcatcggcgg actcgcgcga
      961 ttcaacgggc agaccgtctt cgtgctcggg catcaaaagg gccgagacac gaaggagcgg
     1021 gcactgcgca acttcggcat gagcaagccg gagggctatc gcaaggcgct gcgcctgatg
     1081 aagctcgcgg agaaattcgg cctgccggtt ttcactttcg tcgacacccc gggggcgtat
     1141 ccgggcatcg acgccgagga gcgcggccag tcggaggcga tcggccacaa cctctacgag
     1201 atgtcgcaac tcaaggtccc gatcatcacg acgatcatcg gcgagggcgg gtcgggcggc
     1261 gcgctcgcca tcgcggtggc cgaccaggtc ctgatgctgc agtacgcgac ctactcggtg
     1321 atctcgccgg agggctgcgc gtcgatcctc tggaagagcg ccgacaaggc gccggaagcg
     1381 gccgaggcgc tgggactgac ggcgcatcgc ctgaaggcgc ttggcctgat cgacaagatc
     1441 gtctccgaac cgctgggtgg cgcgcaccgc gacagcaagc agatgtcggc attgctgaag
     1501 cgtgggctgg cggacgccct gcgccagttc accggcatga agccgcgcga actggtcgcg
     1561 gcccgccacg cgcgactgct tgcctatggc cgcttcaaga acacgaccga agagacctga
     1621 tccgctcgac gaactgctgg tgaacgcggc cagcgcggcg ctgcggcgcg cgcaggcgcg
     1681 gctcgaggcc atcgatcgca gtggcgatga ggcgttgcgg cggcgttcgg ctcgtcgtca
     1741 ggcgatcacc gcggttgtcg gttacagcgg cggtcgcgac tccacggcac tgctgcaggt
     1801 catggcggga ctggcacgga agcgcggcac gggcctgagc gaagtgatcg ccgtgcatgt
     1861 acatcacggc atcagtcgcc attccgacgc gtggctggcg cactgcgagg ccggggccac
     1921 ggccctgggc cttcgttttg tcgggcgccg ggtcacggtg cagcgggcgg ggcggggcct
     1981 cgaggcagca gcgcgcgatg cgcgttacga ggcattggca gaagtcgcgg ccgacgtcgg
     2041 cgcacaggtc gtctgctgcg cgcatcaccg tgacgatcga gtcgagacct ttctgattca
     2101 gtggatgcgc ggcgccggcc ccgagggcct ggcggcattt ccaccgtcgc gtcgcttcga
     2161 tgccgacggg ctgctgctgg ttcggccgtt catcgatatc gcacgggccg acatcgaacg
     2221 ctacgtcgat cgacacggac tcgactacat cgaggacgac agtaacgacg atcgaagcct
     2281 gctgcgcaat gcgttgcgac tcgacgtgct gccacgcata gaggcccttc ggccgggatt
     2341 tcgcgccgcg gcggcgcggt cggtggatct ggtggcggaa gccaccgagg cactgcgcag
     2401 cgtcgccgat gacgacctgg cgctctgtca ggacgccgcg cccgtgggca tgctgcggct
     2461 cgacgtgctg gcaacgctgc ccgcggcgcg acagacctgg gtgctgcgtg cctggctggc
     2521 atcgcacggt ctcgaagccc tctcgcgggc acgactggtc gagatgctgg agcaggcccg
     2581 cgatgcgcgc agcgatgcgc ggctgcttgt gcgcatcggc gcgcgtgaga tccggcgcta
     2641 ccgcggattg ctgctgctca aggacagcga cgccggggcg ggcgacgccg aggcgttccg
     2701 ctggaccggc gaggacgaga ttgcgctgcc cgggtggggc ggggtgctgc gatttcatcg
     2761 cacgcaggat cgcgagggat tcgaacccga ctggctttcc gcgatgccgc tcgaagcacg
     2821 gccacgcgct ggaggagagc gcttcaagcc tcacgccggt cgcccgtcgc gaacgctcaa
     2881 gcgcctttac caggacgccg gcatcgccga ctttgaccgt gcgcgcctgc ctctgctgtg
     2941 gcgcgatggg gaactgatct tcgtcgccgg cctcggtgca gacgtcagac tcacggatcg
     3001 cgacggtgaa cgcgtcacga tcgagtggca gcccgacgcc acgctgatcg gcgtggactg
     3061 agcgggcgca tcaaggcgcc aagtttcgac ccgaccgagt cgattgctcc ggatcgcggt
     3121 tccggtgcgt ctccccgtgc cgcgactgac gcggatcatt cggaatcgct gtccttcgca
     3181 cctataatcg cgagttgatc agtttccgca gcgaatttcc gtagagaaca gcagcgcggc
     3241 catggctctc atcgttcaca agtacggcgg cacctcggtc ggcacagtcg accggatcaa
     3301 gaacgtcgca cgccgaatcg agaagtggca caaggccggc caccagctcg tcgtcgtcgt
     3361 gtcggccatg agtggcgaga ccaatcggct cctgggcctg gccaaggaag tccagtcgaa
     3421 tcccgatccg cgcgaactcg atgtcgtcgc gtcgaccggt gaacaggtca cgatcgggtt
     3481 gctgtcgatg gccctgaagg accgtgggct tctggccaag agctacacgg gtccgcaggt
     3541 ccacgtgctg acggacagtg cattcaccaa ggcacgcatt ctcgagatcg acgagaagaa
     3601 catccgtcgc gacctcgagc agggggcgat cgtcgtcgtt gccggcttcc agggcgccga
     3661 cgagtacggc aacatcacga cgctcgggcg cgggggctcc gacacatcgg cggtcgcgct
     3721 cgcggcggcg ctgaaggccg acgagtgcct catctacacc gacgtcgacg gtgtttacac
     3781 caccgacccg cgcatcgttc cggaagcacg ccggctggac aaggtcacct tcgaggagat
     3841 gctcgaaatg gcgagcctcg gctccaaggt gctgcagatc cgttccgtcg agttcgccgg
     3901 caagtacaag gtgccgttgc gcgtgttgtc gagcctgacg gagcccgggc tgccgctggc
     3961 cgaggaggcc tcgtccggca cgctgatcac tttcgaggaa gcctcaatga tggaaaaggc
     4021 agtcatctcc ggcgtcgctt tttcgcgcga cgaggccaag atcaccctga cacgagtgcc
     4081 cgaccgcccc ggcatcgcct accagatcct tggtgcgatc gccgacgcca acatcgatgt
     4141 cgacatgatc gtgcagaaca tcagcgtcga ggggacgacc gacttttcct tcacggtcca
     4201 ccgcaacgag taccagaagg cgctcgacgt cctcaacacc caggtcaagg atcacgtcgc
     4261 cgcgaagcag atcgtcggcg acccgaagat cgccaaggtc tcggtcgtcg gcatcggcat
     4321 gcgctcgcac gtcggcatcg ccagcctgat gttccgaacc ctgtcggaag aagggatcaa
     4381 catccagatg atctccacca gcgagatcaa gatctccgtc gtggtcgagg acaagtacat
     4441 ggaactcgcg gtgcgagccc tgcacaaggc gttcggcctc gagcaggcgc cggtctgaga
     4501 attgctgcgg gcttggcgaa cgcctagaat ccgcaaaggc gcgggcacga tcccgcgcaa
     4561 acttttttgg agacgtggcc gagaggtcga aggcactccc ctgctaaggg agcatgcgcg
     4621 caaaaacgtg catcgacggt tcgaatccgt ccgtctccgc catcacaaac gcccccttta
     4681 cgggggcgtt ttcatttcct gataccgcat cgcggctagc tcatctgcag actcgcctgc
     4741 gcgcagcgac ccgttgaata atccgcccag ttcacactcg accggagatg cgcgtgaaga
     4801 tgttgcgaat aatcctgccc gttgccgcgc tgctggccgc ccatggtgcg ctggccgcgg
     4861 gtgtcggcgt ccgcgccggc acgaccggca tcggcgcgga tgttgccgtc gaagttttgc
     4921 ccacgctgtc ggcgcgggtc ggttattcct acctcagctt cagcacctcg gtcgattcgt
     4981 ccgaggtcga gtacgacgcc aagccgaagc tgtcgaatgg caacctgttc cttgactgga
     5041 gtccgctcgg cccattccgc atcaccggcg gcctgatcct gaatgacaac aaggtcgatg
     5101 tcactggcgt cccgaccggg ggcacattca ctctgaacgg cgtggtttac ccggcagcgg
     5161 cgatcggcag tctctcgggc acggtcaagt cgggcaagtc ggcggcgccg tacctaggca
     5221 tcggctatgg caacgtatcg ggagccggcg tcaatgtcta cttcgacctc ggcgtgatgt
     5281 tcccgggcaa tcccaaggcc acgctgacgg cgacctgcgg cccatcattg cccccggcac
     5341 aatgcagcca actgcagtcc aacgcggcgg ccgaagcgat cgacctgcag gacagcatca
     5401 agaacttcaa gtacttcccg gtggccagct ttggtgtgac gatcggcttc tagacccgct
     5461 gccgcgaagg cagttcgtcg cgaggtcgca tggtaaactc gcgacctcgc
//
LOCUS       DATIRT010000419         1786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_169384, whole genome shotgun sequence.
ACCESSION   DATIRT010000419 DATIRT010000000
VERSION     DATIRT010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169384"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..1107)
                     /locus_tag="VLN25_09435"
     CDS             complement(22..1107)
                     /locus_tag="VLN25_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HSQ10819.1"
                     /translation="MLQRLLLKLCCAFGGLVLVSAPAQGQAQTPITFKVHHFLPPGST
                     THAKLIVPWCARIEAESAGRMRCQIYPSMQLGGTAPQLYDQVRDGVVDIVWTLPGYNA
                     GRFPSMEVFELPFMIGDAGSASRAAWQYYEKHGRSEFRDIRPLAIHVHDPGQLHTREK
                     PVRTLEDFRGMKLRAPTRQTNKLLSALGAAPVSMPVTGLADAMSKGVLDGAVVPWEVV
                     PAVKVHEVARFHTETDPNMPALYTAVFLFAMNSKTYASLPADLRIIIDRNSGVELSTQ
                     AGRLWDEQTAPARKLALDRGNTIIVVPAAELARWQRAAQSVQSEWIVDMNRRQLDGNE
                     LLADARALLARGAAATARGAPRERTSK"
     gene            complement(1128..>1786)
                     /locus_tag="VLN25_09440"
     CDS             complement(1128..>1786)
                     /locus_tag="VLN25_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HSQ10820.1"
                     /translation="PIDGTADLFPVHRIYCVGRNYADHAKEMGFSEREAPFFFTKPAD
                     AVLPVPFGATGEMPYPGETANLHHEIELVVAIGRGGRDIAQADAASHIWGYAVGLDMT
                     RRDLQFALRDKGRPWDVAKAFDFSAPISPLKPKSATGEITTASIWLEVNGQKRQSSDV
                     AKLIWSVNETIEHLSKFFVLVPGDLIFTGTPEGVGAVKPGDLMAGGIAGIGTLQVRVV
                     "
BASE COUNT          325 a          592 c          555 g          314 t
ORIGIN      
        1 cgcgtagcgc gcgccccatg attacttcga agtccgctcg cgcggcgcgc ctcgtgccgt
       61 ggccgcggca ccgcgcgcca gcagcgcacg cgcatcggcg agcagctcgt tgccgtcaag
      121 ttgacgacgg ttcatgtcga cgatccactc ggactgcacc gactgggccg cgcgctgcca
      181 tcgcgccagc tcggccgcgg gcaccacgat gatggtgttg ccgcgatcga gcgccagctt
      241 gcgcgccggc gccgtctgtt cgtcccacag ccgtcccgcc tgagtggaaa gttccactcc
      301 cgagttgcga tcgataatga ttcgcaagtc cgcaggcaga ctcgcgtagg tcttcgagtt
      361 catcgcgaac aggaacactg ccgtgtacaa cgcgggcatg ttggggtcgg tctccgtatg
      421 gaagcgcgcc acctcgtgga ccttgacggc cggcactacc tcccagggca ctacggcccc
      481 gtccagcacg cccttgctca ttgcgtcagc gaggccggtg accggcatcg acaccggcgc
      541 agcgcccagc gccgacagca gcttgttcgt ctggcgcgtt ggagcacgca gcttcatccc
      601 gcgaaagtct tcgagcgtac gcaccggttt ttcgcgcgta tgcagctgac cggggtcatg
      661 cacatgaatc gccagtggcc tgatgtcccg gaactcggac cggccgtgct tttcgtagta
      721 ctgccaggca gcacgcgagg ccgacccggc gtcaccgatc atgaatggca actcgaacac
      781 ttccatcgat gggaaccgac cggcgttata gccgggcaaa gtccacacga tatcgacaac
      841 gccgtcgcgg acctggtcgt acagctgcgg tgccgtgcca ccgagctgca tcgacggata
      901 gatctggcat ctcatccgcc cggcagattc ggcttcgatc cttgcgcacc acggcacgat
      961 cagcttggca tgcgtcgtgg aaccgggggg caggaaatgg tgcaccttga acgtgatcgg
     1021 cgtttgcgcc tgcccttgcg ccggggccga taccaagacc aggccaccga acgcgcagca
     1081 aagcttgaga agcagtctct gaagcatggc gcgacgccgt ggtggaccta gaccacgcgg
     1141 acctgcaagg tgccgatgcc ggcgatgccg cccgccatca ggtcgcccgg cttgactgct
     1201 ccgacgccct cgggcgtccc ggtaaagatc aggtcaccgg gaaccagcac gaagaatttc
     1261 gagagatgct cgatggtctc gttcacgctc cagatcagtt tggcgacatc gctcgactga
     1321 cgtttctggc cgttgacctc gagccagatc gacgccgtcg tgatctcgcc ggtcgccgac
     1381 ttcggtttca gcggcgagat cggcgccgag aaatcgaacg cctttgcgac gtcccaaggt
     1441 cgacccttgt cgcgcaatgc gaactgcagg tcacgccgcg tcatgtcgag tccgaccgca
     1501 tagccccaga tatggctggc cgcatcggcc tgagcgatat cgcggccgcc gcgaccgatg
     1561 gcgacgacga gttcgatctc atgatgcaaa ttggcggttt cgccgggata cggcatctcg
     1621 cccgtggcgc cgaagggcac aggcagcacg gcgtcggccg gtttggtgaa gaaaaacggt
     1681 gcctcgcgtt cggagaatcc catctccttc gcatgatcgg catagttgcg accgacacag
     1741 tagatccgat gcaccggaaa cagatccgcc gtgccgtcga tcggca
//
LOCUS       DATIRT010000420         3531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1744255, whole genome shotgun sequence.
ACCESSION   DATIRT010000420 DATIRT010000000
VERSION     DATIRT010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1744255"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VLN25_09445"
     CDS             complement(<1..440)
                     /locus_tag="VLN25_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10821.1"
                     /translation="MIRLLCRPLLAALVLGLLSGCATLSGEPTQKVNIQTVDAEGKAI
                     DGMSCRASNSSAKYVGVTPMFDMHVRRSSSALVVECRRDGYPLSRAVVVSRAVNMQLL
                     QMLLPGGSSLMVVAHLTGYMYAYPRWVRLQVGTDMVFDRRDEVDR"
     gene            652..993
                     /locus_tag="VLN25_09450"
     CDS             652..993
                     /locus_tag="VLN25_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10822.1"
                     /translation="MNALRLLAPAIALLVLAAHFWRAQWWPLAALCVALVGLLALRRA
                     WVARLVQTALMLGTIEWLRTLAALAATRMALGQPYTRMSLILGAVAVATLASALVFRA
                     RALRERYGLGR"
     gene            complement(1003..2193)
                     /locus_tag="VLN25_09455"
     CDS             complement(1003..2193)
                     /locus_tag="VLN25_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HSQ10823.1"
                     /translation="MQLIDRVVQFRTEIQQIRRDIHAHPELRFEENRTADIVAAKLTE
                     WGISVTRGLGGTGVVGTLRHGTSTRSVGLRADMDALPIQELNDFAHRSTHAGKMHACG
                     HDGHTAMLLAAARYLAETQPFDGSVHLIFQPAEEGGAGAQRMIDDGLFERFPCDAVFG
                     MHNWPNLKVGQFGVAPGPAMASANEFEITIKGKGAHAAMPHLGVDPVFVAVQVVNGLQ
                     AIVTRVKKPIDAAVLSVTIIRAGDATNVVPDSAFISGTVRTFSDAVTAQIDEHMRRIV
                     QMTAAAHGAESHVDFQWNYPPTVNHATEAEFAATVMDDIVGADNVVRGIEPTMGSEDF
                     AFMLKVRPGAYVFIGNGDGTHRASGHGLGPCMLHNPSYDFNDELIPLGATFWIRLVEK
                     YLGR"
     gene            2322..3521
                     /gene="argE"
                     /locus_tag="VLN25_09460"
     CDS             2322..3521
                     /gene="argE"
                     /locus_tag="VLN25_09460"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HSQ10824.1"
                     /translation="MPAEAVVPSAVALEMIDRLIAFPTVSRDSNLGLIEFVRDELARL
                     GVRSQLIYDVSKKKANLFATLSSEPDRLKSGGLVLSGHTDTVPVDGQNWSSDPFVATH
                     RDGRIHGRGTADMKGFIAIALAWAPRFLAAQDRLPVHLSLTYDEETTFLGVRGLVADL
                     AERGIRPAGCLIGEPTDMQAIVAHKGKRDFCCRVRGRESHSALTPEGVNAIEFAARLI
                     AFIRNLADRLSRDEPRDARFAVPYSTLQTGVISGGIAVNVVPRDCEFRFEMRNLPATS
                     QDTLSQEIMAFAERHLVPEMRVVAVDSGIDFELGMNVPAFGIAPDAPLVLWAQQVART
                     ADRGAGAVSFGTEASIFSEAGIPTVVLGPGSIEQAHKPDEYISYGQVEACELLFERMI
                     AEPWMPQ"
BASE COUNT          578 a         1143 c         1198 g          612 t
ORIGIN      
        1 cgatcgacct cgtcgcggcg gtcgaacacc atgtcggtgc cgacctgcag ccgcacccag
       61 cgcggatacg cgtacatgta gccggtcagg tgggcgacca ccatcaggct ggagccgccg
      121 ggcagcagca tctgcaacag ctgcatgttg acggcccgcg aaaccacgac cgcgcgcgac
      181 agcgggtagc cgtcacgacg gcattcgacg accagcgccg acgacgagcg gcgcacatgc
      241 atgtcgaaca tcggcgtgac gccgacgtac ttcgccgacg agttgctggc gcggcagctc
      301 atgccgtcga tcgccttgcc ttcggcgtcg acggtctgga tgttgacttt ctgtgtcggc
      361 tcgccgctga gcgtcgcgca gcccgacagc aggccgagca ccagcgcggc gagcagtgga
      421 cggcaaagaa gacggatcat cggtaagcgc ctggcgatcg ggacgggtac agcggctgca
      481 ggggacagtg cgggccagcg taccggggga gcaaggacga caccctccgg cgaacggatt
      541 agctgggggc cggctccagc accctctttc ggggtaggcg agggtcgacc ctgatacgaa
      601 tcaagcacct ggcggcccgg atcgcggacc atccgcgccg gagacgccac cttgaacgcc
      661 cttcgcctgc ttgcccctgc gatcgccctg ctggtgctgg cggcgcactt ctggcgcgcc
      721 cagtggtggc cgctcgcagc gctctgcgtc gctctggtgg ggctgctcgc gctacgccgg
      781 gcgtgggtgg cacgactggt gcagaccgca ctgatgctcg ggacgatcga atggctgcgt
      841 acgctggcag cgctggcagc gacccggatg gccctcggtc agccgtatac ccggatgagc
      901 ctgatcctcg gcgcggtcgc ggtggccacg ctggccagcg cgctggtatt ccgtgcacgt
      961 gcgctgcggg agcgctacgg cctcggccga tgaagtgacc gcctagcggc cgagatactt
     1021 ctcgaccagc cggatccaga aggtcgcgcc caggggaatc agctcgtcgt tgaagtcgta
     1081 gctggggttg tgcagcatgc aaggcccgag cccgtgaccg gatgcgcgat gcgtgccatc
     1141 gccgttgccg atgaacacgt aggcgccggg ccggaccttg agcatgaagg cgaagtcctc
     1201 cgagcccatg gtcggctcga ttccccgcac gacgttgtcg gctccgacga tgtcgtccat
     1261 cacggtggcg gcgaactcgg cctcggtcgc gtggttgacc gtcggcgggt agttccactg
     1321 gaagtcgaca tgcgactccg ctccatgcgc cgccgcggtc atctggacga tgcgacgcat
     1381 gtgctcatcg atttgcgcgg tcaccgcatc gctgaaggtg cgcacggtgc cggatatgaa
     1441 cgcgctgtcc ggcaccacat tggtcgcgtc gccggcacga atgattgtca cggacagcac
     1501 cgcggcatcg atcggcttct tgacgcgggt gacgatggcc tgcagaccgt tgaccacctg
     1561 cacggcgacg aacaccgggt cgacgccgag gtgcggcatc gctgcatgcg cgcccttgcc
     1621 cttgatggtg atctcgaact cgttcgctga cgccatcgcc gggccgggcg cgacaccgaa
     1681 ttgcccgacc ttcaggttcg gccagttgtg catgccgaac accgcatcgc acggaaagcg
     1741 ctcgaacagt ccgtcatcga tcatgcgctg tgccccggcg ccgccttcct cggccggctg
     1801 gaagatcagg tgcacggacc cgtcgaaggg ctgcgtctcg gccaggtaac gggcggccgc
     1861 cagcagcatt gccgtgtggc cgtcgtggcc gcatgcgtgc atttttcccg cgtgggtcga
     1921 gcgatgcgcg aaatcgttga gttcttggat cggcagcgcg tccatgtcgg cgcgcagccc
     1981 gaccgagcgc gtggacgtgc cgtggcgcag ggtcccgacc acgccagtgc cgcctagccc
     2041 gcgggtcacc gagatgcccc attcggtgag cttggcggcc acgatgtccg cggtgcgatt
     2101 ttcctcgaag cgcagttcgg gatgggcgtg aatgtcacga cggatctgct ggatctcggt
     2161 gcggaactgc acaactcggt cgatcagttg catggaaaac ctcagggtgg ggcatcgatc
     2221 gtactgaagc tcgcgccggc gcgcacccgg gtcaccccta cgcgcacccg tccaggccct
     2281 agaatcgaat cgatcccgca aggacttccg tgaacaagcc gttgcctgcc gaagctgtcg
     2341 tgccatccgc cgtcgctctg gaaatgatcg accggctgat cgcgtttccc acggtcagcc
     2401 gcgactccaa tctcgggctg atcgaattcg ttcgcgacga gttggcccgg ctcggcgtcc
     2461 gctcgcaact gatctacgac gtttcgaaga agaaggcgaa tctctttgcg acgctgtcgt
     2521 ccgaaccgga tcgtctgaag agcggtggac tcgtgctgtc cggccacacc gacacggtgc
     2581 cggtcgatgg ccaaaactgg agcagcgatc cgttcgtggc aacgcaccgc gacggccgca
     2641 ttcatggtcg tggcacggcc gacatgaagg gcttcatcgc gatcgcgctg gcctgggcgc
     2701 cgcgcttcct ggcggcgcag gatcgcttgc cggtgcacct gtcgctgact tatgacgagg
     2761 agaccacctt tctcggcgtg cgcggcctgg tggcggatct ggccgagcgc ggcatccggc
     2821 ccgcgggttg cctgatcggc gagccgaccg atatgcaggc gatcgtggcg cacaagggta
     2881 agcgcgattt ctgttgccgg gtgcgcgggc gcgaatcgca ttcggcgctg acgccggagg
     2941 gggtcaatgc gatcgagttc gcggcgcggc tgatcgcgtt catccgcaac ctggccgatc
     3001 gcctgtcgcg cgacgaaccg cgcgatgcac gcttcgcggt tccgtacagc acattgcaga
     3061 ccggtgtgat cagcggcggc attgcggtca acgtcgtgcc gcgcgattgc gaattccggt
     3121 tcgagatgcg caatctgccc gcaacgtcgc aggacaccct tagccaagaa atcatggcct
     3181 tcgccgagcg ccatctcgtt cccgagatgc gggtggtggc ggtcgattcg ggcatcgact
     3241 tcgaactcgg tatgaacgtg ccagcgttcg gcatcgcgcc cgatgcgccg ctagtcctgt
     3301 gggcacagca ggtggcgcgc accgccgacc gtggtgccgg cgcggtcagc tttggtaccg
     3361 aggcgagcat cttttccgaa gccggcatcc cgaccgtggt gctgggcccg ggctcgatcg
     3421 agcaggcgca caaacccgac gagtacatca gctacgggca ggttgaggcg tgcgagctac
     3481 tgtttgagcg catgatcgcc gagccatgga tgccgcaatg aagaatttgc a
//
LOCUS       DATIRT010000421         7585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1350470, whole genome shotgun sequence.
ACCESSION   DATIRT010000421 DATIRT010000000
VERSION     DATIRT010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7585
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350470"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VLN25_09465"
     CDS             <1..422
                     /locus_tag="VLN25_09465"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019248911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HSQ10825.1"
                     /translation="RTREVQVAQEILQTLGLRAFTPLVVACPGCGRTTSTFFQELADS
                     IQTWLRGQMPIWRRDHPGVESMSVAVMGCVVNGPGESKHANIGISLPGSGEAPVAPVF
                     VDGVRTVTLKGEHIAEEFQAIVDDYVRRKYVRATTPA"
     gene            429..1742
                     /gene="hisS"
                     /locus_tag="VLN25_09470"
     CDS             429..1742
                     /gene="hisS"
                     /locus_tag="VLN25_09470"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HSQ10826.1"
                     /translation="MATRKLAGVRGMNDLLPADSALWEWFEARVADWLKSYGYQQIRT
                     PIVEHTELFVRGIGEVTDIVEKEMYSFEDKLNGEKLTLRPEFTAGVIRAVVEHNLTYD
                     APKRVWSMGPVFRHERPQRGRYRQFYQVNVEAVGFAGPDIDAELIVMLARLWRILGVD
                     RVGRVRLELNCLGQADERERHRADLIAHLSRHESRLDADARRRLHTNPLRILDSKNPD
                     LAEIIALAPRITDYLGDASRAHFEGLQSLLKAAGIEFTVNPRMVRGLDYYNLTVFEWI
                     TDALGAQGTLCGGGRYDPLIERLGGRPTPGIGFGMGIERVIELLRESASVDVNARTDA
                     YVLHQGGATQQPAFLLAERLRDAGMDVILHAGDASLKSQMKKADASGAEYAVIVGETE
                     AVASAATVKALRAGHPAAPFAEQRIVPLDQIPEALVDALTRNDDE"
     gene            1750..2388
                     /locus_tag="VLN25_09475"
     CDS             1750..2388
                     /locus_tag="VLN25_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSQ10827.1"
                     /translation="MAYDLEEQEQLAELKAWWQKYGNLVLTAVTAVLLAFAAYNGWRW
                     YEREQARSAAGIYGQLEQAVQARDAAKVQTLASSIVEHYGRSIYAVLGALQAAKLNAE
                     SGDLPKAAEQLRWVIDKSGHDELTMIARVRLAGVLLDEKKFDQALQVLNFDAPASYLP
                     TVLDRRGDVLVAQNKNVEARVAYAEALAKADAQHPLRAIIQLKLDALPPATS"
     gene            2385..3584
                     /gene="bamB"
                     /locus_tag="VLN25_09480"
     CDS             2385..3584
                     /gene="bamB"
                     /locus_tag="VLN25_09480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HSQ10828.1"
                     /translation="MTVVARRSMQQRCAAGAGRALSALLGALVLAGCSSMPSWMPSWM
                     GGSPSRSAEPAPLVEFSPSLSPQVAWRTNVGSSRGADLQPAVLENAIYAASASGSVVR
                     IGPNGETVWRTETNVAIAGGVGSDGFVIAVGSTRGEVIALGADGKELWRAQVSGVVQS
                     PPLVGRGLVIVRASDHRISAFDSSSGKRRWNYQRTTPALTLQSITQMAFAGELVAAGF
                     PSGRLVMLVAANGTVRWDVPVAEPRGSSEVERLTDVIGGPLVIGGDVCAAAYQGRVAC
                     FEASNGNLRWARDLSAGAGVGGDSRRIYAVDAKSNVVAFSSATGANAWRQEKLLNRDL
                     TTPLALRRAVVVGDLAGYVHFLSPDDGAFLARVALGSEINAAPRAFGGGAIVQTQDGT
                     VALLTVE"
     gene            3624..4961
                     /gene="der"
                     /locus_tag="VLN25_09485"
     CDS             3624..4961
                     /gene="der"
                     /locus_tag="VLN25_09485"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HSQ10829.1"
                     /translation="MKPVIALVGRPNVGKSTLFNRLTRSRAALVADFPGLTRDRHYGD
                     GRVGPRDYIVIDTGGFEPLNAEGIAREMAAQAEQAIAESDAIVFVLDAREGVTAQDRR
                     IGERLRVTGRTVLLAVNKAEGLAPEAIAEFHELGLGEPYAISAAHGEGVRDLMDLALD
                     PFVVAEPEAVDDAETAAGQRRIRVAVVGRPNVGKSTMINALLGEERLIAFDQPGTTRD
                     AIEVEFEFDGRPYTLIDTAGLRRRGKVFEAVEKFSVVKTLQAIADANVVILLLDASAE
                     ISDQDAHIAGYVLESGRALVIAINKWDGLDQYQRDRIKREIERKLHFLRWAKVHTISA
                     LKRTGLSAVMRSVTEAHAAAFAKLPTPKLTRALQAAVTRQMPPRSGSLRPKMRYAHQG
                     GMNPPVIVIHGNSLNHVPDTYRRYLEGHFRAEFELAGTPLRVEFRTGRNPYVS"
     gene            5102..5335
                     /gene="hfq"
                     /locus_tag="VLN25_09490"
     CDS             5102..5335
                     /gene="hfq"
                     /locus_tag="VLN25_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HSQ10830.1"
                     /translation="MSNKGQLLQDPFLNALRKEHIPVSIYLVNGIKLQGQIESFDQYV
                     VLLRNTVTQMVYKHAISTVVPARPVNLAAESDS"
     gene            5383..6498
                     /gene="hflX"
                     /locus_tag="VLN25_09495"
     CDS             5383..6498
                     /gene="hflX"
                     /locus_tag="VLN25_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008542638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HSQ10831.1"
                     /translation="MLVSVAQGRRDDPDAVEELTQLVMSAGLVPSAHIAVQRDRPDPA
                     HYVGAGKVEEIKSLAASGDAGLVVFDVALSPAQQRNLERTLGLWVLDRTALILDIFSQ
                     RARSREGKLQVELAQLQHLSTRLVRGWSHLERQRGGLGKTGGPGEKQIEIDRRLIGVR
                     VKQLRERLKQLERQRKTRRRARARRDVLSVSLVGYTNAGKSTLFNALTRAGAFAADQL
                     FATLDTTARRLYLGAGEAAVISDTVGFIRGLPHQLVEAFKSTLDETAEADLLLHVVDT
                     ASSARADQMAEVDRVLDEIGAASVPRLIVFNKIDASGREPSVERDPYGRIVAVSVSAK
                     TGLGLDLLRDAIVEFAHQTREAAPPESTPAWSSAAVV"
     gene            6559..>7585
                     /gene="hflK"
                     /locus_tag="VLN25_09500"
     CDS             6559..>7585
                     /gene="hflK"
                     /locus_tag="VLN25_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HSQ10832.1"
                     /translation="MSLNDPQWGRGGGSGGSNGDGEREAPRRPQRPNGEGPPDLEELW
                     RDFNRRLNGLFGKKGSGSRGGGGGFGNLGASPRGAGIGFAALLGVLGLIWVGSGFYIV
                     PEGQVGVVTTFGRYSETTSPGFRWRMPWPVQASELVNILALQKVEVGARGRPDRVKEA
                     LMLTDDENIVDIHFEVQFRIKDDGARDFLFNSKNPTAAVIQSAESAMREVLGRQKMDS
                     VLYESRQQIADEVRVRIQEMLDRYGTGILVSAVAIQNAQPPEQVQAAFDDAVKAGQDR
                     ERQINEGQAYANDVVPKARGAASRLMQEAEGYRTRIVQTAEGDASRFRQILTEYQKAP
                     AVTRDRMY"
BASE COUNT         1329 a         2378 c         2596 g         1282 t
ORIGIN      
        1 cgcgtacgcg cgaagtccag gtcgcgcagg aaatccttca gaccctgggc ctgcgtgcct
       61 tcacgccgct ggtcgtggcc tgccccggct gcggccgcac caccagcacg ttctttcagg
      121 aactggccga ctcgatccag acgtggctgc gtggccagat gccgatctgg cgccgcgatc
      181 acccgggtgt cgagtcgatg agcgtcgctg tcatgggatg cgtggtcaac gggcccggcg
      241 aaagcaagca cgccaatatc ggcatcagcc tgcccggctc cggggaagcg ccggtggcac
      301 cggtgttcgt cgacggcgtt cgcaccgtga cgctgaaggg cgagcacatc gccgaggaat
      361 tccaggcgat tgtcgatgac tacgtgcgcc ggaagtacgt gcgcgcgacg actccggcct
      421 gaccgacgat ggccactcga aagcttgccg gtgtgcgcgg catgaacgat ctgttgccgg
      481 ccgattccgc gctgtgggag tggttcgagg cgcgtgtggc cgactggctg aagagctacg
      541 gctatcagca gatccgcacg ccgatcgtcg aacacacgga gctcttcgtg cgcggcatcg
      601 gcgaggtcac cgacatcgtc gagaaggaga tgtactcctt cgaggacaaa ctcaacggcg
      661 agaaactgac gctgcggccc gaattcaccg ccggcgtgat ccgtgccgtg gtcgaacaca
      721 acctcaccta tgacgcgccc aagcgtgtct ggtcgatggg accggtgttc cgtcacgagc
      781 ggccgcagcg tggacgttac cggcagttct accaggtcaa tgtcgaagcg gtgggcttcg
      841 ccggacccga tatcgatgcc gaactgatcg tcatgctggc gcgcctctgg aggatcctcg
      901 gcgtcgaccg cgtcggacgt gtgcgactcg agctcaactg cctcggacag gccgatgagc
      961 gggagcggca ccgcgccgac ctgatcgcac acctgtcgag acacgagtcc aggctcgacg
     1021 ccgatgcccg gcgccgactg cacaccaacc cgctgcgcat tctggacagc aagaatccgg
     1081 acctggccga gatcattgcc ctcgcacctc gcatcaccga ctacctcggt gacgcgtcgc
     1141 gggcccactt cgagggcctg cagtcgctgc tgaaggcggc cggcattgag ttcacagtca
     1201 acccgcgcat ggtgcgcgga cttgattact acaacctcac ggtgttcgag tggatcaccg
     1261 atgcgcttgg tgcgcagggg acactgtgcg gcgggggccg ttatgacccg ctgatcgaac
     1321 gtctgggcgg cagaccgacg ccgggcatcg gtttcggcat gggcatcgag cgcgtcatcg
     1381 aactcctccg cgagagcgcg tccgtcgatg tgaacgcgcg caccgatgcc tatgtcctgc
     1441 atcagggcgg cgcgacgcag cagccggcat tcctgcttgc cgagcggttg cgggacgctg
     1501 gcatggacgt gatcctgcat gcgggcgatg ccagcctgaa atcgcagatg aagaaggccg
     1561 atgcgagcgg cgcggaatac gccgtgatcg tcggcgaaac cgaggcagtt gccagtgcgg
     1621 cgacagtgaa ggcgctgcgc gctggacatc cggcggcgcc cttcgccgag cagcgtatcg
     1681 tgccgctcga ccagattccc gaggcgctgg tggacgcgct gacacgcaac gacgacgaat
     1741 aggaagacga tggcgtacga ccttgaagaa caggagcaac tggccgagct gaaggcctgg
     1801 tggcagaagt acggcaacct ggtgttgacc gcggtgactg ccgtgctgct ggcgttcgcc
     1861 gcctacaacg gctggcgctg gtacgagcgc gaacaggcca gatcggcggc gggcatctat
     1921 ggccagctgg agcaggccgt gcaggcgcgc gatgcggcga aggtgcagac actggccagt
     1981 tcgatcgtgg agcattacgg acgctccatc tacgccgtgc tgggcgcgct gcaggcggca
     2041 aagctgaatg ccgagagcgg tgatctgccc aaggcggcgg agcagttgcg gtgggtcatc
     2101 gacaagtcgg ggcatgacga gctgacgatg attgcgcgtg tccgcctggc cggtgtcctg
     2161 ctcgacgaaa agaagttcga ccaggcgctg caggtgctga acttcgatgc gccggcgtcg
     2221 tacctgccga cggtgctgga ccggcgcggt gacgtgctgg ttgcacaaaa caagaacgta
     2281 gaggcgcggg tcgcctacgc ggaagcgctc gccaaggcgg atgcccagca cccgctgcgc
     2341 gccatcatcc agctcaagct cgacgcgctg ccgccggcga catcatgacc gtcgtggcgc
     2401 gccgatcgat gcagcagcgc tgtgccgcag gcgcaggtcg cgctctgtcg gcattgcttg
     2461 gtgccctggt gctggccggg tgttcctcca tgccgtcctg gatgccgtcc tggatggggg
     2521 gctcgccaag ccgatcggcg gaacccgcgc cgctggtcga gttctcgcct tcgctgtcgc
     2581 cccaggtggc gtggcgcacc aacgtcggat cgtcgcgcgg cgcggacttg cagccggcag
     2641 tgctcgaaaa cgcgatctac gcagcgagcg cgagtgggtc ggtggtgcgg atcggcccga
     2701 acggggaaac agtgtggcgc acggagacga acgtcgcgat cgcgggtggc gtcggcagtg
     2761 acggattcgt gatcgcggtt ggcagtacgc gcggcgaggt cattgccctc ggcgctgacg
     2821 gcaaggaact ctggcgggcc caggtgagtg gagtggtgca gtcgccgccg ctggtgggac
     2881 ggggactggt catcgtgcga gcgtccgatc atcggatttc ggcgtttgac tcgagcagcg
     2941 gcaagcgccg ctggaactat cagcgcacca cgccggcgct gacgctccag tcgattactc
     3001 agatggcatt tgcaggcgag ctggtcgctg cgggttttcc cagcgggcgg ctggtgatgc
     3061 tggtcgcggc gaatggcacc gtacgctggg acgtcccggt ggcggagccg cgcggttcgt
     3121 ccgaagtcga gcgcctgacc gatgtgatcg gcgggccact cgtgatcggc ggcgacgttt
     3181 gtgccgcggc ctatcagggc cgggtggcgt gcttcgaggc gagcaatgga aacctgcgct
     3241 gggcgcgcga cctgtccgcc ggggccggtg tcggtggcga ctcccgccgg atttacgcgg
     3301 tcgacgccaa gtcgaacgtg gtcgctttct cgtccgcgac cggtgcgaac gcctggcgcc
     3361 aggagaagct gctcaaccgg gacctgacca cgccactggc gctgcggcgt gcagtggtcg
     3421 tcggcgacct tgccggctac gtccactttc tttcaccgga cgacggtgcc ttccttgcgc
     3481 gagtggccct gggcagtgag atcaacgcgg cgccgcgtgc gttcggtggc ggcgcgatcg
     3541 tgcagaccca ggacggaacc gttgcactgt tgaccgtcga gtgacgcagt tcagcagcgc
     3601 accgaatccc tgagccccgt tcgatgaaac cggtcatcgc gcttgtcggc agaccgaacg
     3661 tcggcaagtc gacgctgttc aatcgcctca cgcgctcgcg cgctgcactg gtcgccgatt
     3721 tcccaggact gacgcgggac cgtcactacg gggacggccg cgtcggaccg cgcgactaca
     3781 tcgtcatcga cactggcggg ttcgagccgc tgaacgccga gggcattgcc cgggaaatgg
     3841 cagcgcaggc cgagcaggcg atcgcagaaa gcgatgcgat cgtcttcgtg ctcgatgcgc
     3901 gcgaaggcgt gacagcccag gatcgtcgca tcggcgagcg actgcgcgtg accggacgaa
     3961 cggtgctgct ggccgtgaac aaggccgagg gcctggcgcc cgaggcgatc gcagagtttc
     4021 acgaactcgg actcggtgaa ccgtacgcga tctcggccgc gcacggtgaa ggggtgcgcg
     4081 acctgatgga cctcgccctt gatccctttg tcgtggcgga accggaggct gtcgacgacg
     4141 cggagactgc ggcgggacaa cggcgcatcc gcgttgcggt ggtggggcgc cccaatgtcg
     4201 gcaagtccac gatgatcaat gccctgcttg gtgaggaacg gttgatcgcg ttcgatcagc
     4261 cgggcacgac gcgcgatgcc atcgaagtcg agttcgagtt cgacggccgg ccgtacacgc
     4321 tgatcgacac ggccggactg cgccgccgcg gcaaggtctt cgaggcagtt gagaagttct
     4381 cggtggtcaa gacactgcag gcgatcgccg acgccaatgt cgtgatcctg ctgctcgacg
     4441 cgtcggccga gatctcggac caggatgcgc acatcgccgg ctacgtgctc gagagcggcc
     4501 gggcgctggt catcgccatc aacaagtggg acgggctcga ccagtaccag cgtgaccgca
     4561 tcaagcgtga aatcgagcgc aagctccact tcctgcgctg ggccaaggtt cacacgatct
     4621 cggcactcaa acggaccgga ctgtcggcgg tcatgcgctc ggtgaccgag gcacacgcgg
     4681 cggcgttcgc aaagctgccg acgcccaagc tcacgcgcgc cttgcaggcg gcggtcacgc
     4741 gccagatgcc gccacgttca ggctcgctgc gccccaagat gcgctacgcg caccagggtg
     4801 gcatgaaccc gccggttatc gtgatccacg gcaactcgct gaaccacgtt cctgacacct
     4861 accggcgtta cctggagggc catttccgtg ccgagtttga gctggccggc acgccgctgc
     4921 gtgtcgaatt ccggacagga cgcaacccct atgtcagctg agcgcgttgg tggtcaaggg
     4981 cgggcttttc ctatacattg aaccgcgtgg ttccgtcccc atttagcaac ctgtgacggt
     5041 cggttcactt cctccgtgaa ccgaatacaa caagcggcgt caaagcgctg gagccaacaa
     5101 aatgagcaac aaagggcagt tactacaaga cccgtttctt aacgcgctgc gcaaggaaca
     5161 cattccggtc tcgatatacc tcgtgaacgg catcaagctg cagggtcaga tcgagtcgtt
     5221 cgatcagtat gtcgtgctgc ttcggaacac agtgacccag atggtctaca agcatgcgat
     5281 atccactgtc gtgccggcgc gtcccgtcaa cctcgccgcg gagtccgact cctgaccgtc
     5341 ttccaggttg aaagcgagcg cgccggtcgg tccgagcgcg cgttgctggt cagcgttgcc
     5401 cagggaaggc gtgacgaccc ggatgcagtc gaggaattga cgcagctggt gatgtcggcg
     5461 gggctggtgc cttccgccca catcgccgtg caacgcgatc gaccggatcc cgcgcattat
     5521 gtcggcgccg gcaaggttga agaaataaag tcccttgctg cgtcgggcga tgccggtctg
     5581 gtggtctttg acgtggcgct gtccccggcg cagcagcgaa acctggagcg gacgctcggg
     5641 ttatgggtgc tggaccggac cgcactgata ctggacattt tctcgcagag ggcgcgcagt
     5701 cgcgaaggca agctgcaggt ggaactggcg cagctgcagc acctgtcgac gcgactggtc
     5761 cgaggctggt cgcacctgga gcgccagcgc ggcggactcg gcaagaccgg cggaccgggt
     5821 gaaaagcaga tcgagattga ccggcgcctg atcggtgtgc gggtcaagca gctgcgcgag
     5881 cggctcaagc aactcgaacg ccagcgcaag acgcgtcgcc gggcgcgtgc ccggcgggac
     5941 gtgctctcgg tatcgctggt cggatacacg aacgccggca agtcgacgct gttcaacgcg
     6001 ctgacccgcg cgggcgcctt tgcggccgac cagctcttcg ccacgcttga tacgaccgcg
     6061 cgtcgcctct atctgggtgc cggcgaggct gcggtcattt cggacacggt cgggttcatc
     6121 cgtggtctgc cgcatcaact cgtcgaggca ttcaagtcca cgctcgacga gaccgccgag
     6181 gcggacctgc tgctgcatgt cgtcgacacc gcctcttcgg cacgagcgga ccagatggcc
     6241 gaagtcgacc gcgtgctcga tgaaattggc gctgcctcgg tgccgcggtt gatcgtcttc
     6301 aacaagatcg acgcgtcggg ccgcgagccg tcggtcgagc gtgaccccta tggtaggatc
     6361 gtcgccgttt cggtcagcgc gaagaccggc ctgggcctcg atctgctgcg cgatgcgatc
     6421 gtcgagtttg cacatcagac ccgcgaggcc gcacctcccg aaagcacccc tgcctggtcc
     6481 agtgccgccg tggtctagca tcgcggccgc gatgcattga tgattcttat ggtcaaccca
     6541 tttttcgcta ggacacgcat gtcgcttaac gacccccagt ggggccgagg tggcggttcc
     6601 ggtggctcga acggtgacgg cgagcgcgaa gctccgcgcc gcccgcagcg cccgaacggc
     6661 gaaggcccgc ccgatctcga ggaactctgg cgcgatttca atcgccgcct gaacggcctg
     6721 ttcggtaaga aaggttccgg ctcgcgcggc ggcggtggcg gattcggcaa cctcggtgcc
     6781 agtccacggg gcgccggcat cggcttcgcg gccctgctcg gtgtactggg cctgatctgg
     6841 gtcgggagcg gcttctacat cgtgcccgag ggccaggtag gggttgtcac cacgtttggt
     6901 cgctactcgg aaaccaccag cccgggattc cgctggcgga tgccgtggcc ggttcaggcg
     6961 agcgagctcg tcaacatcct ggcactgcag aaggtggagg ttggcgcgcg tggccgcccg
     7021 gatcgtgtca aggaagcctt gatgctgacc gacgacgaaa acatcgtcga tattcacttc
     7081 gaagtacagt ttcgcatcaa ggacgatggc gcgcgcgact tcctcttcaa cagcaagaat
     7141 ccgacggcgg ccgtgatcca gtccgccgaa tcggcgatgc gcgaggtcct gggacggcag
     7201 aaaatggact cggtcctcta tgagtcgcgt caacagattg ctgacgaagt gcgggtccgc
     7261 atacaggaaa tgcttgatcg ctacggcacc ggcatcctcg tttcagccgt cgcaatccag
     7321 aacgcccagc caccggagca ggtgcaggcc gcattcgacg atgcggtcaa ggccggacag
     7381 gaccgggagc gccagatcaa cgaggggcag gcatatgcca acgacgtcgt gccgaaggcg
     7441 cgcggcgccg cctcgcgcct gatgcaggaa gccgagggtt accggacacg gatcgttcag
     7501 acggccgagg gcgacgcctc gcgcttccgc cagatcctga cggagtacca gaaggcgcct
     7561 gcggtaacgc gtgaccgcat gtacc
//
LOCUS       DATIRT010000422         3535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_71499, whole genome shotgun sequence.
ACCESSION   DATIRT010000422 DATIRT010000000
VERSION     DATIRT010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71499"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="VLN25_09505"
     CDS             complement(<1..1147)
                     /locus_tag="VLN25_09505"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HSQ10833.1"
                     /translation="MPEFFINGQPVSAAKGQTVMQAARAAGYFIPTFCWHPNLSVSGN
                     CRICVVEVEGEGGGGSWMEIACNMPVTEGMRVLTDSEAVRARRRETLQFILLNHPVDS
                     GICDKAGECTLQDYHYEMNGLPSVSTVPKVQATKYFELSSRIVLDNERCIVCSRCVRF
                     TREISKSHALGIQNRGDSSLVRPVENTDFEADPYSDNVIDICPVGALLSKQFLHKARV
                     WYLKATPSVCPGCSRGCSIDLWHRKSEWKLNALDPRENSAIARVTPRENPAVNGPWIC
                     NKGRDLARIFERPRAEGAMLNGRPALLDDAIAVARALIRKARRPVALVSSWGSNEELA
                     AFKSTLGERFECIVKSDCQAEPGERLEDDVLIRPDKNPNTRAAQELFG"
     gene            complement(1140..2456)
                     /gene="nuoF"
                     /locus_tag="VLN25_09510"
     CDS             complement(1140..2456)
                     /gene="nuoF"
                     /locus_tag="VLN25_09510"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HSQ10834.1"
                     /translation="MTGRKLLMSFPVTPTSHRLDEVRARGGYQTLQRVLTSMTPEQVT
                     KEVTAAGILGHGGAAFPVGRKWSVIHLNDSQPHYLCANADEGEPGTFKDRWILENSPH
                     QLVESMVIAAYALQVRNAFVYIRGEFDLPYRRLQDAVDEAYAAGFLGEKILGTDFDLD
                     LVVYRGAGSYVCGEASALITSIEGKKGYPRNRPPRLTVRGLYQRPTVINNVETLANVA
                     IIVGMGAEEFRKVGIAKSPGTQLVSISGHIEKPGVYEVEYGYPLAKFIYEDCGGVLGG
                     KGLKCIIPGGISTKMLTAADIATVTYDHKAMEAVGSQVGSGGMVVIAEGTCMVRLLQV
                     MLRFYHHESCGQCTPCREGMGWMHRIIDRIVAGQGRPEDIDTLIRVSNAKDGTTICGM
                     GDAAGFATVGIIAKYRDEFEYYIANGRSKNGGRLEVGVMEREAAHA"
     gene            complement(2453..2917)
                     /gene="nuoE"
                     /locus_tag="VLN25_09515"
     CDS             complement(2453..2917)
                     /gene="nuoE"
                     /locus_tag="VLN25_09515"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HSQ10835.1"
                     /translation="MRHLLPEFEQLKKRYPAGFESSLTLPCLRRIQKERGYVADEDID
                     WLVEYLGVPRIQIEEVLSFYTQYRRQPIGRWHLQTCHNVSCSMRGAERLIDHLARRLG
                     IQPGETTPDGRFTLSHVECLGSCGTAPVVMVNDAYHENMNAEKVDALLGQLK"
     gene            complement(2923..>3535)
                     /locus_tag="VLN25_09520"
     CDS             complement(2923..>3535)
                     /locus_tag="VLN25_09520"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HSQ10836.1"
                     /translation="LIDRNRIFIDRTRDVGVLSTADAINWGYTGPILRSTGAPRDLRK
                     DTPYLAYAELDFEVPVGIKGDNYDRYYVRMREMDESVHMIRQLIDMLPAGPIDVDDRR
                     CTFPPKPLVYNEIESLINHFKLVMEGPRVPAGEIYVAHEGANGELGFFLVSDGSGTPH
                     KVHVRSPSFVHMGGVHRMLDGYQLADLIPTFGSINMIGGECDR"
BASE COUNT          601 a         1163 c         1100 g          671 t
ORIGIN      
        1 tgccgaaaag ttcctgcgct gcgcgcgtgt tcgggttctt gtcggggcgg atcagcacgt
       61 cgtcttccag ccgctcgccg ggctccgctt ggcagtcgga cttgacgatg cattcgaagc
      121 gctcgcccag cgtcgacttg aacgccgcca gttcctcgtt cgatccccag ctcgacacca
      181 gcgccaccgg ccggcgagct ttgcggatca atgcgcgcgc gaccgcgatt gcgtcgtcca
      241 gcagtgccgg acgcccgttg agcatcgccc cctcggcgcg cggacgttcg aagatcctgg
      301 ccagatcgcg gcccttgttg cagatccacg gtccgttgac ggccgggttc tcgcgtggcg
      361 ttacgcgcgc aatggcgctg ttctcgcgcg gatcaagcgc gtttagtttc cattccgact
      421 tgcggtgcca caggtcgatc gaacaacccc ggctgcaccc cgggcacacc gaaggcgtcg
      481 ccttcaggta ccagacgcgc gccttatgca ggaactgctt ggaaagcagc gcgccgaccg
      541 ggcagatgtc gatgacattg tcggagtaag gatcggcctc gaagtcggtg ttctcgaccg
      601 gacgaaccag cgaactgtca ccgcggttct ggatgccgag cgcgtgcgac ttcgagatct
      661 cgcgcgtgaa gcggacgcag cgcgagcaca cgatgcagcg ctcgttgtcg agcacgatcc
      721 ggctcgacag ctcgaagtac ttcgtggcct gcaccttggg caccgtcgac accgacggca
      781 gcccgttcat ctcatagtga tagtcctgca gcgtgcactc gcctgccttg tcgcagatgc
      841 cgctgtcgac cgggtggttc agcaggatga actgcagtgt ctcgcggcgt ctcgcccgta
      901 ccgcctcgga atccgttagc acgcgcatgc cttcggtcac gggcatattg caggcaatct
      961 ccatccagct gccgccaccg ccctcgcctt cgacctcgac gacgcagatc cggcagttgc
     1021 cggacaccga caggttcggg tgccagcaga aggttgggat gaagtagccc gcggccctgg
     1081 ccgcctgcat cacggtctgg cccttggccg cgctgaccgg ctgcccgttg atgaagaact
     1141 caggcatgcg ccgcctcccg ctccatcacg ccgacctcga gccgcccgcc gttcttcgac
     1201 cgtccgttgg cgatgtagta ctcgaactcg tcgcggtact tggcgatgat gccgacggtc
     1261 gcgaagccgg ccgcgtcacc catgccgcag atcgtcgtgc catctttggc attggaaaca
     1321 cggatcaacg tgtcgatgtc ctcgggtcga ccctggcccg cgacaatgcg gtcgatgatc
     1381 cggtgcatcc agcccatgcc ttcacggcac ggcgtgcact gcccgcacga ctcatggtgg
     1441 tagaagcgca gcatcacctg cagcaggcgc accatgcagg tgccctcggc aatgaccacc
     1501 atgccgccgg aaccgacctg cgagcccacg gcctccatcg ctttgtggtc gtaggtcacc
     1561 gtcgcaatgt ccgcggccgt cagcatcttt gtcgagatgc cgccgggaat gatgcatttc
     1621 agccccttgc cgccgagcac gccgccgcag tcttcgtaga tgaacttcgc cagcgggtag
     1681 ccgtactcga cctcgtacac cccgggcttt tcgatgtgtc cggagatcga cacgagttgc
     1741 gtgcccgggc tctttgcgat gcccaccttg cggaactcct cggcacccat gccaacgatg
     1801 atggcaacgt tggccagtgt ctccacgttg ttgatgacgg tcggccgctg gtacagcccg
     1861 cgtacggtca atcgaggcgg ccggttgcgc ggatagcctt tcttgccttc gatcgatgtg
     1921 atcagtgcgg acgcttcgcc gcagacatac gatccggcgc cgcgatagac cactaggtcg
     1981 aggtcaaaat cggtcccaag gatcttttcg cccagaaacc cggccgcgta agcctcgtcc
     2041 accgcatcct gcaaccggcg atacggcagg tcgaactcgc cgcggatgta gacgaaggcg
     2101 ttgcgcacct gcagcgcgta ggccgcgatc accatcgact cgaccagttg gtgcggcgag
     2161 ttctcgagga tccagcgatc cttgaacgtg ccgggctcgc cctcgtcagc gttggcgcac
     2221 aggtagtgcg gctgggagtc gttcagatgg atgaccgacc acttgcgacc gaccgggaag
     2281 gccgcgccgc catgcccgag gatgccggcc gccgtgactt ccttcgtcac ctgctccggc
     2341 gtcatcgacg tcagcacgcg ttgcagcgtc tggtagccgc cgcgggcgcg cacctcgtcg
     2401 agccgatgcg aggttggcgt gaccggaaac gacatcagca gcttacgacc agtcatttca
     2461 gttgccccag cagcgcatcg accttctctg cattcatgtt ctcgtggtag gcgtcgttca
     2521 ccatcacgac cggcgcggtt ccgcacgagc cgaggcattc gacgtgtgac agcgtgaagc
     2581 gtccgtccgg tgtcgtctcg cccggctgaa ttccgaggcg cctagccagg tggtcgatga
     2641 ggcgttccgc gccacgcatc gagcacgaga cgttgtggca ggtctgcagg tgccagcggc
     2701 caatcggctg gcgccggtac tgcgtgtaga acgacagcac ttcctcgatc tggatgcgcg
     2761 gcactccgag gtactcgacc agccagtcga tgtcctcatc cgccacgtag ccgcgctcct
     2821 tctggatgcg ccgcaggcaa ggcagtgtca atgaagactc gaaacctgcc gggtagcgct
     2881 tcttgagctg ctcgaactcc ggaagcagat gtctcatcgt gctcacctgt cacactcccc
     2941 gccgatcatg ttgatcgagc cgaaggttgg gatcaggtcg gcgagctggt agccgtcgag
     3001 catccggtgc acgcctccca tgtggacgaa cgatggcgag cgcacgtgca ccttgtgcgg
     3061 ggtgccgctg ccgtcactga ccaggaagaa gccgagttcg ccgttggcgc cttcatgcgc
     3121 tacgtagatt tcgccggccg gcacacgcgg accttccatg accagcttga agtgattgat
     3181 cagcgattcg atctcgttgt agacgagcgg cttgggcggg aatgtgcagc gacggtcgtc
     3241 gacgtcgatg ggaccggccg gcagcatgtc gatcagctgg cggatcatgt gcacggactc
     3301 atccatctcg cgcatgcgca cgtagtaacg gtcgtagttg tcgcccttga tgccgaccgg
     3361 cacctcgaag tcgagctcgg cataggccag gtacggcgtg tccttgcgca ggtcacgcgg
     3421 cgcaccggtc gaacgcagga tcggcccggt gtagccccag ttgatcgcat ccgcagtcga
     3481 gaggacgccg acgtcgcggg tccggtcgat gaaaattcga ttacggtcga tcagg
//
LOCUS       DATIRT010000423         1963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1252869, whole genome shotgun sequence.
ACCESSION   DATIRT010000423 DATIRT010000000
VERSION     DATIRT010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252869"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VLN25_09525"
     CDS             <1..183
                     /locus_tag="VLN25_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10837.1"
                     /translation="RSAFGLADPDHRAQACRRRKASEGSESGQTATNMAPQRKVINQG
                     AAKTQRDSHSELESPA"
     gene            complement(444..1679)
                     /gene="fabF"
                     /locus_tag="VLN25_09530"
     CDS             complement(444..1679)
                     /gene="fabF"
                     /locus_tag="VLN25_09530"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HSQ10838.1"
                     /translation="MSGKRRVVVTGLGVISPLGNDVPSTWAALLEGRSGIGPITRFDA
                     SGLNCRIAGEVKGFDVTTVMSAKDARHVDLFIHYGVAASAEALKDSGLEISDANRDRI
                     GCVIGSGIGGLPMIEENHTQLLNRGPRRVSPFFVPGSIINMIAGTVSIRHGLRGPNLA
                     IVSACTTGLHSIGDAGRLIEYGDADVMLAGGAEGSTCPLGVGGFDSMKAMSTRNDDPT
                     TASRPFDRDRDGFVLAEGAGIVVLEEYEHARRRGARIYCELVGYGMSADAHHMTAPDM
                     DGPRRCMLVALKNAGVSADEVDYLNAHGTSTPLGDINETKAIKAALGERARAIVVNST
                     KSMTGHLLGGAGGIESVFTALAVHHQVSPPTMNIFNQDPECDLDYCANSARAMKIDVA
                     LKNSFGFGGTNGTLVFKRH"
     gene            complement(1713..1955)
                     /gene="acpP"
                     /locus_tag="VLN25_09535"
     CDS             complement(1713..1955)
                     /gene="acpP"
                     /locus_tag="VLN25_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HSQ10839.1"
                     /translation="MENIEQRVKKIVAEQLGVNEADIKNESAFVEDLGADSLDTVELV
                     MAREDEFECEIPDEEAEKIRNVQQAVDYINSHLKKA"
BASE COUNT          365 a          650 c          599 g          349 t
ORIGIN      
        1 cgctctgctt tcggccttgc tgatcccgac caccgcgcac aggcatgcag gcggcgaaag
       61 gcatccgaag gcagtgaatc cggccagaca gcgaccaaca tggcaccaca acgcaaggtt
      121 atcaaccaag gggcggcgaa aacacagcgc gactcgcact ccgaactgga atccccagct
      181 tgaatgcgcg tccagaccgc accgtcggca tcgatcttca cctcccggct gccgccggac
      241 gacgggcggg cgcgcaccag tgcccagacg gaagccgcag ctgacacgag agccgcgctc
      301 cagaggtgtc cgggatggcg cggatcggca accaggacgg cgacggccag cacgcaggcg
      361 gaaaccgctg tcgccaccac ggccgcgagc cagagccgcc gttcccgcgc cgatggctgc
      421 aggacggtcc agccgaggat cactcaatgt cgcttgaaga ccagggtccc gttcgttccg
      481 ccgaagccga acgagttctt gagcgcgacg tcgattttca ttgcgcgtgc cgagttggcg
      541 caatagtcga gatcgcactc cggatcctgg ttgaagatat tcatggtcgg cggtgatacc
      601 tggtgatgaa ccgcgagcgc ggtgaacacc gactcgatgc caccggcacc gccgagaaga
      661 tggcccgtca tcgatttcgt cgagttgacg acgatggcgc gggcccgctc gccgagggct
      721 gccttaattg ccttggtctc gttgatgtcg cccaacggcg tcgaggtacc gtgcgcattc
      781 agatagtcga cctcgtcggc actaacgccg gcattcttca gggcgacgag catgcagcgc
      841 ctcggaccgt ccatgtcggg agccgtcata tggtgcgcgt ctgcgctcat gccataaccg
      901 accagttcgc aatagatgcg ggcaccgcga cggcgcgcgt gctcgtattc ctccagcacg
      961 actatgccgg caccctccgc caggacgaag ccatcacgat cgcggtcgaa cggccggctg
     1021 gcggtcgtcg gatcgtcgtt ccgcgtcgac atggccttca tgctgtcgaa gccgccgacc
     1081 ccgagaggac acgtcgagcc ctccgcaccg ccggccaaca tcacgtccgc gtcgccgtat
     1141 tcgatcagtc gaccggcatc gccgatcgaa tgcaggccgg tcgtgcaggc gctgacgatc
     1201 gcgagattcg gaccgcgcaa gccatgccgg atcgagaccg tgccggcgat catgttgatg
     1261 atcgagccgg gaacgaaaaa tggcgatacg cggcggggtc cgcgattgag cagctgcgtg
     1321 tgattttcct cgatcatcgg caacccgccg attcccgagc cgatcacgca accgatgcga
     1381 tcgcgattgg catcgctgat ctccagcccg ctgtccttaa gggcttcggc cgaggcggcc
     1441 acgccatagt ggatgaacaa atcgacgtgg cgcgcgtcct tggccgacat cacggtcgtg
     1501 acatcgaagc ccttgacctc ccccgcgatg cgacagttca ggcctgacgc atcgaagcgc
     1561 gtgatgggcc cgatacccga acggccttcg agcagtgccg cccacgtgct tggcacgtca
     1621 ttgccaagcg ggctgatgac gccaaggccg gtgacgacga cgcgacgctt gcctgacatc
     1681 gaacctctgc tgaatgctgc cagcggcccc gttcaggcct tcttgaggtg actgttgatg
     1741 tagtcgacgg cctgctgcac gttgcggatc ttctcggctt cctcgtccgg aatctcgcac
     1801 tcgaattcgt cctcccgcgc catcacgagc tcgacggtgt cgagcgagtc ggcaccgagg
     1861 tcctccacaa aggccgactc gttcttgata tcggcttcgt tgactccgag ttgctcggcg
     1921 acgatcttct tgacgcgttg ttcaatgttt tccatcgact cct
//
LOCUS       DATIRT010000424         7110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1646005, whole genome shotgun sequence.
ACCESSION   DATIRT010000424 DATIRT010000000
VERSION     DATIRT010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7110
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1646005"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VLN25_09540"
     CDS             complement(<1..743)
                     /locus_tag="VLN25_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSQ10840.1"
                     /translation="MSHFLDRLTHFTLPKEEFANGHGVATGEDRTWEDAYRNRWSHDK
                     IVRSTHGVNCTGSCSWKIYVKGGIVTWETQQTDYPRTRADLPNHEPRGCARGASYSWY
                     LYSANRLKYPMVRGRLLERWRAAMQVAKDSGKGAVDAWASIVEDPAARRDYQKVRGMG
                     GFVRSNWDEVNQLIAAANVYTIKVHGPDRVVGFSPIPAMSMVSYAAGSRYLSLIGGVC
                     MSFYDWYCDLPPASPQVWGEQTDVPESAD"
     gene            complement(766..2412)
                     /locus_tag="VLN25_09545"
     CDS             complement(766..2412)
                     /locus_tag="VLN25_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiporter"
                     /protein_id="HSQ10841.1"
                     /translation="MAAAQPVGAAPRPTSSVDIVDWRPEDNAFWDSTGKKIANRNLWI
                     SVPSLLCGFAVWLMWGIITVQMANLGFPFTQQQLFTLAAISGLSGATLRIPASFFIRI
                     SGGRNTIFLTTALLMIPALGTGIALTDKNTPLWVFQFMALLSGIGGGNFACSMSNINT
                     FFPKRLQGTALGINAGLGNFGVTTMQILIPLVMTTAVFMTLGGDPMTLVKPSGTLIGK
                     IPAGTPTYIQNAGYIWLVLLVPLAFVGWFGMNNLLTISPNIGSTAAALGKILLLYGVA
                     LVTAAIGLYLYLPSTGLGLLNMWVAMPLIIIATLLAMKLVAFGDMKPNLKKQFEIFKD
                     KHTWSMTLLYIVTFGSFIGFSNALPLSINVIFGQSHVLGPDGVLKHLPNPNAPSALTY
                     AWIGPFVGALIRPVGGWISDKVGGSIVTQIISVVMVIASAAVGYVMMLAYASATPEQY
                     FLPFIGLFIVLFAASGIGNGSTFRTVGVIFDRVQAGPVLGWTSAIAAYGSFIAPIVIG
                     EQIRAGTPQYAMYGFAIFYALCLILNWWFYLRRNAYVKNP"
     gene            complement(2448..3731)
                     /locus_tag="VLN25_09550"
     CDS             complement(2448..3731)
                     /locus_tag="VLN25_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HSQ10842.1"
                     /translation="MAAAAQRAEASTTSQKTQQWSVAIMSTIAFTVCFAIWMMFAVIG
                     IPIKKMLDLNATQFGLLTATPVLTGSLIRVPLGMWTDKYGGRIVFFLLMISTVIPIWL
                     LTYATAYWHFLVLGLFVGLAGGSFSVGTPYVARWFDKKHQGFAMGIFGAGNSGAAVTK
                     FVAPALVVGFGWALVPQVYAVAMLVTAIAFWFFTYSDPKHLIPGHITYRRQLEVLRDP
                     AVWKYCQYYSIVFGGYVALSLWMVMYYVGEFGLDIRIAALLAACFSLPGGVLRAIGGW
                     MSDKWGAHKVTWWVMWVSWICLFLLSYPATTYTITTVNGPYTFTLAHNVWTFTTLMAV
                     LGVAWAFGKASVFKYIADDYPTNIGTISGIVGLAGGLGGFVLPIMFGALLDLTGVRSS
                     AFMLMYGVVWVSLIWMYRTEVSTRDVVHSEPKPAT"
     gene            3966..5873
                     /locus_tag="VLN25_09555"
     CDS             3966..5873
                     /locus_tag="VLN25_09555"
                     /inference="COORDINATES: protein motif:HMM:NF025055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HSQ10843.1"
                     /translation="MTSVSWFRRLWSGSTLVRVGVVLSVVAMLALSVIVAATIFAEQS
                     TGKASAINIAGSLRMQSYALSTRVADIRGSTTDRHEAAEAAIVEFERRLKSPALMTGI
                     PDNGANPVHRIYSGIVHDWESRIKPLARRAIEDEAERANFLTEVPTFVAKVDILVRQI
                     ETDLEGRIQLLRMVLGIGFFVMMMLVVAALFLLNVEVFQPIARLGEAAKRVRTGDFTV
                     RAGSTGPDEIGQLGQAFNFMVDDLARLYGSLEKQVAEKTLDLERRNESLSLLYETTRM
                     LAEKPLDSASLSDVLAMIRKALGVESGVICSIHAGGVRGMPLARDEGVVKACMERDCH
                     LCAGDGGVTWRTEPSDSGDRRVIGIPLVDGGVRYGVMPLALPPNRGLEVWQMELAETM
                     GRHVGAALASAERREEHRRLGVLEERAAIARELHDSLAQSLSYTKIQIARLSATLERD
                     GNGEQSAAVLAELREGIATAYRELRELLTTFRLGLGEEGMAGTLRDTVAEFERRSGVP
                     VELANDLLKVELSANEQVHVLQVVREALTNIEHHAHARRAWVALRRDDDHSIEVSVED
                     DGIGIGQGRSPHGHFGLAIMRDRAASVGGDITIEARVPRGTCVRLRFRPQTAFARAGV
                     AANASRAGAAA"
     gene            5870..6529
                     /gene="narL"
                     /locus_tag="VLN25_09560"
     CDS             5870..6529
                     /gene="narL"
                     /locus_tag="VLN25_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HSQ10844.1"
                     /translation="MNENRYTILVVDDHPLFRKGVIQLLALEPKFEVVGEAGDFASAV
                     RLAKEREPDLTLLDLNLKGSGGLEILAALKDEDPSQRVVMLTVSDAPEDLMAAIRAGA
                     DGYLLKDTEPEELLERVRGALEGRTVISDALTAALADALRDEARGVRRDVLDLTDREL
                     AVLRCVAGGQSNKVIARNLSITEGTVKVHVKHLLKKLGFRSRVEAAVWASEQGLRSAT
                     A"
     gene            6918..>7110
                     /locus_tag="VLN25_09565"
     CDS             6918..>7110
                     /locus_tag="VLN25_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10845.1"
                     /translation="MNLPATSPSPISIAGFKDVYEIPRVEVALSDLQELGNGANEALR
                     ATYVKMIRSGGQRFVIKPSA"
BASE COUNT         1324 a         2185 c         2398 g         1203 t
ORIGIN      
        1 cagtcggcgc tctccggcac atcggtctgc tcgccccaga cctgcgggct ggccgggggc
       61 aggtcgcagt accagtcgta gaagctcatg cagacgccgc cgatcagtga gagatatcgg
      121 cttccggccg cgtagctgac catgctcatt gccgggatcg gcgagaagcc gaccacgcga
      181 tcggggccgt ggaccttgat cgtgtagacg ttggcggcgg cgatcagctg gttgacttcg
      241 tcccagtttg aacgcacgaa gccgcccatg ccgcgcacct tctggtagtc acgtcgcgcg
      301 gccgggtctt cgacgatcga ggcccatgca tcgacggcgc cttttcccga atctttcgcg
      361 acttgcatcg cggcgcgcca gcgctcgagc agtcggccgc gcaccatcgg gtacttcagc
      421 cggttggcgc tgtagaggta ccaggagtac gaggcgccac gggcgcagcc gcgcggctcg
      481 tggttcggca ggtcggcacg cgtacgcggg tagtccgtct gttgtgtctc ccacgtgacg
      541 atgccgcctt tgacgtagat cttccacgaa caggacccgg tgcagttcac gccgtgtgtg
      601 gagcgcacga tcttgtcgtg cgaccagcgg ttgcggtagg cgtcttccca ggtgcggtct
      661 tcgccggtcg ccacgccgtg gccgttggcg aattcctcct tcggcagcgt gaagtgcgtc
      721 aggcgatcga gaaagtgact catgtgtgtc tccgcagcag ggggatcagg ggttcttcac
      781 gtacgcattg cggcgcaggt agaaccacca gttcaggatc aggcaaagcg cgtaaaagat
      841 ggcgaagccg tacatcgcgt actgcggcgt gccggcccgg atctgctcgc cgatgacgat
      901 cggcgcgatg aacgagccgt aggcagcgat cgcggaggtc cagcccagga ccggcccggc
      961 ctgcacgcga tcgaagatca cgcccacggt gcggaaggtc gagccattgc cgatgccgct
     1021 ggcggcgaac agcacgatga acaggccgat gaacggcagg aagtactgct cgggcgtggc
     1081 cgaggcgtac gccagcatca tcacgtagcc caccgcagca gacgcgatca ccatcacgac
     1141 cgagatgatc tgagtgacga tcgagccgcc gaccttgtcc gagatccagc cccccaccgg
     1201 ccggatcagc gcgccgacga aggggccgat ccacgcatag gtcaatgccg acggtgcatt
     1261 cggattcggc aggtgcttga gcacgccatc ggggccgagc acgtggctct ggccgaagat
     1321 cacgttgatc gacagaggca gagcgttgga aaagccgatg aaggagccga aggtgacgat
     1381 gtacagcagc gtcatcgacc aggtgtgctt gtccttgaag atctcgaact gctttttcag
     1441 gttcggcttc atgtcgccga aagccaccag tttcatcgcc agcagcgtgg cgatgatgat
     1501 cagcggcatc gcgacccaca tgttcagcag gccgagcccg gtggaaggca gatacaggta
     1561 caggccgatc gcggcggtca ccagtgcgac gccatacagc agcaggatct tgcccagcgc
     1621 ggccgcggtc gagccgatat tgggagagat cgtcagcagg ttgttcatgc cgaaccagcc
     1681 aacgaaggcg agcggcacca gcagcaccag ccagatgtag ccggcattct ggatgtaggt
     1741 tggcgtgccg gccgggatct tgccgatcag cgtgcccgag ggcttgacca gcgtcatcgg
     1801 gtcaccgccc agcgtcatga acacagcagt ggtcatcacc agcggaatca ggatctgcat
     1861 cgtcgtgacg ccgaagttgc cgagtccggc attgatgccg agcgccgtgc cctgcagccg
     1921 cttcgggaag aacgtgttga tgttcgacat cgagcaggcg aaattgccgc cgccgatacc
     1981 gctcagcagc gccatgaatt ggaaaaccca cagcggcgtg ttcttgtcgg tgagcgcgat
     2041 cccggtgccc agcgccggga tcatcaggag cgccgtagtc aggaagatcg tgttgcggcc
     2101 gccggaaatg cgaatgaaaa acgaggccgg gatgcgcagc gtggcgcccg atagtcccga
     2161 gatcgcggcc agcgtgaaca gttgctgctg agtgaacggg aacccgaggt tcgccatctg
     2221 aacggtgatg attccccaca tcagccagac cgcgaaaccg cagagcaggc tcggcacgct
     2281 gatccacagg ttgcggttgg cgatcttctt gccggtcgag tcccagaacg cgttgtcctc
     2341 gggtcgccag tcgacgatgt cgaccgaact ggtgggccgc ggtgcggctc cgacgggttg
     2401 tgcggctgcc atgatgagct ctcctgggtt ctctttgtag gtgcggatca ggtggcgggc
     2461 ttcggctcgc tgtgcacgac gtcgcgcgtg ctcacctcgg tgcggtacat ccagatcagc
     2521 gagacccaga ccacgccgta catcagcatg aaggcactcg aacgcacacc ggtcaggtcg
     2581 agcagcgcgc caaacatgat cggaagcacg aagccgccca ggccgccggc cagcccgacg
     2641 atcccggaga tcgttccgat gttggtcggg tagtcatcgg cgatgtactt gaagacgctc
     2701 gccttgccga aggcccacgc cacgcccagc acagccatca gggtcgtgaa ggtccagacg
     2761 ttgtgggcaa gcgtgaacgt gtacgggccg ttgaccgtcg tgatcgtgta ggtggtggcg
     2821 gggtaggaca gcaggaacag gcagatccag ctcacccaca tgacccacca ggtgaccttg
     2881 tgcgctcccc acttgtcgct catccatcca ccgatggcac gcagcactcc gccgggcaac
     2941 gagaagcacg ccgccaacaa tgcggcaatg cggatgtcga gcccgaactc gccgacgtag
     3001 tacatgacca tccacaacga cagtgcgacg tagccgccga agacgatcga gtagtactgg
     3061 cagtacttcc acacggccgg atctcgcagc acctcgagct ggcggcggta cgtgatgtgg
     3121 cccgggatca ggtgcttcgg gtcgctgtag gtgaagaacc agaacgcgat tgcggtgacc
     3181 agcatcgcca cggcgtagac ctgcggcact aaggcccagc cgaagccgac gaccagcgcc
     3241 ggcgcgacga acttggtcac ggcggcgccc gagttgccgg cgccgaagat gcccatcgcg
     3301 aaaccctggt gcttcttgtc gaaccagcgc gcgacatacg gcgtgccgac cgagaacgaa
     3361 ccgccggcca gccccacgaa caggcccagc acgaggaagt gccagtacgc cgtagcgtag
     3421 gtgagcagcc agatgggaat gacggtgctg atcatcagca ggaaaaagac aatccggccg
     3481 ccgtacttgt cggtccacat gccgagcggc acgcggatca gcgaaccggt cagcaccgga
     3541 gtcgcggtca gcagaccgaa ctgggtcgcg ttcagatcga gcatcttctt gatcggaatg
     3601 ccgatgaccg cgaacatcat ccagatggcg aagcagactg tgaacgcgat cgtgctcatg
     3661 atcgcgaccg accactgctg cgtcttctgg ctcgtcgtgc ttgcttccgc acgctgcgct
     3721 gccgctgcca tcgctgcctc ccggttgaat tgagcgcgac gctaggcggc cggccatgcg
     3781 atctcgtgac gcacgtcaag ccgggcgcgc ggtcacgacc tcagaggaaa gaccccgtac
     3841 ctcgaccggg gtaatcctca taccccgttc cgcgcgacaa tccgtccaca agaggcgggt
     3901 tcacgattga cgccggtcag gcgttgaacg cgtgccgccg ctactgtccg tcgcgggggt
     3961 aatgcatgac gagcgtttcg tggttccgcc gactatggtc cggctcgacg ctggtgcgcg
     4021 tcggtgtcgt gctgtcggtc gtcgcgatgc tggcgctgtc ggtgatcgtt gccgcgacga
     4081 tttttgccga gcagtcgacc ggcaaggcca gcgctatcaa tatcgccggt tcgctgcgca
     4141 tgcagtcgta tgcgctgtcc acccgggtgg cggacatccg cggcagcacc acggatcggc
     4201 acgaggcagc agaggcggcg attgtcgagt tcgagcggcg cctgaagagc ccggcgctga
     4261 tgacgggcat accggacaac ggcgccaatc cggtccatcg catctactcg ggcatcgtcc
     4321 acgactggga atcgcgcatc aagccgctgg cgcgcagggc gatcgaggac gaggccgaac
     4381 gcgccaattt cctgaccgaa gtgccgacct tcgtcgccaa ggtcgacatc ctcgtgcggc
     4441 agatcgagac cgatctggaa ggccgcatcc agctgctgcg gatggtgctg ggcatcgggt
     4501 tcttcgtgat gatgatgctg gtggtggcgg cgttgtttct gctgaacgtc gaggtgttcc
     4561 agccgatcgc gcgcctcggt gaggcggcca agcgcgtgcg cacgggcgac ttcacggtgc
     4621 gggcgggcag cacgggtccc gacgagatcg gtcagctcgg ccaggccttc aatttcatgg
     4681 tcgacgatct cgcgcgactg tatggctcgc tcgaaaagca ggtggccgaa aagacacttg
     4741 acctggagcg ccgtaacgag tcgctctcgc tgctgtacga gaccacgcgg atgcttgccg
     4801 aaaaaccact ggactccgct tcgctttcgg atgtgctcgc gatgatccgc aaggccctcg
     4861 gtgttgaaag cggggtcatc tgttcgatcc atgcaggcgg ggtgcggggc atgccgctgg
     4921 cgcgcgatga aggcgtcgtg aaggcctgca tggagcggga ctgccatcta tgcgcgggcg
     4981 acgggggtgt cacctggcgg acggagccga gcgactccgg cgaccggcgc gtgatcggaa
     5041 taccgcttgt cgacgggggc gtccgctatg gcgtgatgcc gctggcgttg ccgccgaacc
     5101 ggggcctgga ggtctggcag atggagctcg ccgagacgat gggccgtcat gtcggtgcgg
     5161 cactcgcctc ggcggagcgc cgcgaggagc accggcgtct cggcgtgctc gaggagcgtg
     5221 cagccattgc ccgtgagctg cacgattcgc tggcgcagtc gctctcgtac accaagatcc
     5281 agatcgcgcg gctgtcagcc acgctcgagc gcgacggcaa cggcgagcag tcagcggccg
     5341 tgctggcgga actgcgcgag ggcattgcga cagcctaccg cgagttgcgc gaattgctga
     5401 cgacgttccg gctgggactc ggcgaagagg gcatggccgg gacgctgcgt gacacggtgg
     5461 ccgagttcga acggcgttcc ggagtgccgg ttgaactggc gaacgacctg ctgaaggtcg
     5521 agctgtctgc gaacgagcag gtccatgtgc tgcaggtggt ccgcgaagcc ttgaccaaca
     5581 tcgaacatca cgcgcacgcg cgaagggcct gggtggcact gcgacgcgac gacgatcact
     5641 ccatcgaagt ctcggtggag gacgatggca tcggcatcgg gcaggggcgc tcgccacatg
     5701 gtcacttcgg actggcgatc atgcgcgatc gcgcggcatc cgtcgggggg gatatcacca
     5761 ttgaagcccg ggtgccgcgc ggcacctgtg tacgcctgcg cttcaggcca cagaccgcct
     5821 tcgctcgcgc cggtgtcgcg gcgaatgctt cgcgcgcggg ggccgccgca tgaacgagaa
     5881 ccggtacacg atcctcgtgg tcgacgatca cccgttgttt cgcaaggggg tgatccagct
     5941 gcttgcgctc gagccgaaat tcgaagtcgt cggcgaggcg ggcgacttcg cctcggcggt
     6001 gcggctggcg aaggaaaggg agccggacct cacgctgctc gacctcaacc tgaaaggcag
     6061 tggcggcctc gagatcctgg cggcgttaaa ggatgaagac ccgtcgcaac gggtcgtgat
     6121 gctgacggtg tcggacgcgc cggaagacct gatggccgcg atccgcgccg gtgcggatgg
     6181 ctatctattg aaggacaccg agcccgagga gttgctggaa cgcgtgcgcg gtgccctgga
     6241 agggcgcacg gtgatcagtg atgccctgac cgctgcgctt gccgatgccc tgcgcgacga
     6301 ggcgcgcggt gtgcggcgtg acgtgctaga cctgaccgac cgtgagctgg cggtgctgcg
     6361 ctgcgtcgcc ggtggacaga gcaacaaggt gattgcgcgc aacctgtcga tcactgaagg
     6421 caccgtgaag gtgcatgtca agcatctgct gaagaaactc ggattccgct cccgggtcga
     6481 agccgccgtg tgggcctccg agcagggcct gcgctcggcc acggcatagg cgcccccgaa
     6541 tcgctcgttg ccgcaatctt tctgtttgtc ggcagctgac ctctgctgag cgttgaacag
     6601 gtactgagcg ccacaattgg cactccggct agccgagtgc tttgcctttt ggcgtccgat
     6661 ttgcaacatt tctggcaagg gacttgctta ttgaatgatc agacagccat ccagtagcgt
     6721 tacaacggca aggagcactt gtgactgagg accggagggt ttcatgagca tgcggcgcgg
     6781 ccaaccgaag gcgggtgaat tcacacccgg aggaaccgcg cgcagcgcga gggagtccac
     6841 atgagcacgc cgcacagccg cccgaagacg ggcgatgtcc ctcccggagg gaccgcgcgc
     6901 agcgcgaggg agtccgtatg aatctgcctg ccacgagtcc gtcacccatc tcgatcgcgg
     6961 gtttcaagga cgtctacgag atcccgcgcg tcgaagtggc gctgtccgac ctacaggaac
     7021 tgggcaacgg cgccaatgag gcgctgcgcg cgacctacgt caagatgatc cgcagcggcg
     7081 gccagcggtt tgtcatcaag ccctccgcac
//
LOCUS       DATIRT010000425         2178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_71799, whole genome shotgun sequence.
ACCESSION   DATIRT010000425 DATIRT010000000
VERSION     DATIRT010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71799"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VLN25_09570"
     CDS             <1..534
                     /locus_tag="VLN25_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10846.1"
                     /translation="DRFTQTQFWYQKYVEILENLGWATEQFAFTRFDQAEGEFRMDQA
                     ALAIITAIATQNQLAVLQESVAALSSLADDDGTLQLFDFHSSMEGSGNFQLGAVQRSS
                     NGALSLAAGAFYFRSVDERKRFLFFKWGAQQVNFWTAAQRMTLNTEFYARRRAEVVAR
                     LEADAPKFIADLKLGSR"
     gene            complement(572..1705)
                     /locus_tag="VLN25_09575"
     CDS             complement(572..1705)
                     /locus_tag="VLN25_09575"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HSQ10847.1"
                     /translation="MKARAKPAAKSRTAGKKPAKTAARSTARPRKAPTVGGPVTLEEA
                     RALASAAQPKRALRRALAGPPASPASVGEERERLAAQQRAERERRIREYKATMEIMKR
                     RGARRAAPKGAAKGRRRAPGAPRGPAAGFQPLQVFAEGDSWFDYPPFLFKGGVIPRLE
                     SRLGVPILNLAKAGDEVRFMLGVEQRMRLAECLTEGCPAGGPWDLLLFSGGGNDIVDN
                     PMALWVRDWDPTVPPATLINQPRFDAALALVRAGYEDLITLRDMLSPNTHLVFHGYDF
                     AIPDGRGICHLGPWLKPTFDLRGFPTRAAAFAVVEAMLKQFAAMLTTLVAHAKVTFIN
                     GQGTLAPQPSAWHNELHPNKDGFEKFADLFHAQLRAIFPGRVL"
     gene            complement(1752..2129)
                     /locus_tag="VLN25_09580"
     CDS             complement(1752..2129)
                     /locus_tag="VLN25_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10848.1"
                     /translation="MNVLACVTRHWLRDDDTLNIYGWWPDKGEGPVVIFSVAGFDDLP
                     PAGAETDRAIANVLVSGLSGFLGGIDAHERGAKDCPMAVNDARDLKHMVGPQKLDRSC
                     RAKLAKAIPDKLPALDALLKVFR"
BASE COUNT          359 a          746 c          688 g          385 t
ORIGIN      
        1 gaccgcttca cgcagaccca gttctggtac cagaagtacg tcgagatcct cgagaacctc
       61 ggctgggcga ccgagcagtt cgcgttcacc cgcttcgacc aggccgaagg cgagttccgc
      121 atggatcagg ccgcactggc aatcatcacg gcgatcgcca cgcagaacca gctggcggtg
      181 ctgcaggaat ccgtggcggc cctttccagt ctggcggacg acgacggcac cctccagctc
      241 ttcgacttcc actcctcaat ggagggcagc ggcaacttcc agctgggcgc tgtgcagcgt
      301 tcgtccaacg gcgccttgtc cctggcggcc ggagcgttct acttccgcag cgtcgacgag
      361 cggaagcggt ttctcttctt caagtggggc gcacagcagg tgaacttctg gaccgcggca
      421 cagcgcatga cgctcaatac ggagttctat gcgcgccgcc gcgccgaagt cgtcgccagg
      481 ctggaggccg acgccccgaa gttcattgcc gacctgaagc tggggtcccg gtagccacct
      541 caacgcgcac agggacccct gcaaggcacc gctaaagcac gcggccgggg aaaatggcgc
      601 gcagctgcgc gtggaacagg tccgcgaact tctcgaatcc gtccttgttc ggatggagtt
      661 cgttgtgcca ggcactcggc tgcggcgcga gcgttccctg gccattgatg aaagtgacct
      721 tggcgtgcgc gaccagcgtc gtcaacatcg ctgcgaactg cttcagcatc gcttcgacca
      781 cggcgaacgc cgctgcgcgc gtcggaaagc ctcgcaaatc aaacgtcggc ttcagccatg
      841 gccccagatg gcagatgccg cggccatccg ggatggcgaa gtcgtagcca tgaaacacta
      901 ggtgggtgtt tggactcagc atgtctcgca gggtgatcag gtcttcgtaa ccggcacgga
      961 ccagcgcgag cgccgcatcg aagcgcggct gattgatgag cgtagccggc gggacagttg
     1021 ggtcccagtc gcgcacccag agcgccatcg ggttgtcaac gatgtcgttg ccaccgcctg
     1081 agaacaacag caggtcccac ggcccaccgg ccgggcatcc ctcggtcagg cactcggcaa
     1141 ggcgcatgcg ctgctccaca ccgagcatga agcgcacttc gtcaccggcc ttggcgaggt
     1201 tcagtatcgg aacaccgagc cggctttcga ggcgcggaat gacaccgccc ttgaatagaa
     1261 acggcggata gtcgaaccac gagtcccctt cggcgaacac ctggagtggc tggaacccgg
     1321 cggccggacc acgcggcgcg cccggcgcgc gtcgacgtcc tttcgccgcg cccttcggtg
     1381 cggcccgccg cgcaccgcgc cgcttcatga tctccatcgt cgccttgtac tcgcgtatcc
     1441 ggcgttcccg ctcggcacgc tgctgcgccg cgaggcgctc gcgttcctcg cccacggacg
     1501 ccggggacgc aggcggtccg gccagcgcgc ggcgcagtgc acgctttggc tgcgcggcac
     1561 tcgccagcgc ccgcgcttct tcgagcgtga cgggtccgcc gacggtcggt gccttgcgcg
     1621 gtcgtgcggt ggaccgtgct gccgtctttg ccggcttctt gcccgcggtt ctcgactttg
     1681 cggcgggctt cgccctggct ttcaccttcg ttttcgattt cggcttcgtt gccatgtacg
     1741 tcacctcgct ttcagcggaa aactttcagc agcgcatcga gcgccggcaa tttgtccggg
     1801 atcgccttgg cgagcttcgc ccggcaactg cggtccagct tttgcggccc caccatgtgc
     1861 ttcaggtcgc gcgcgtcatt gaccgccatc ggacaatcct tcgcaccacg ctcatgcgcg
     1921 tcgatgccgc cgaggaaacc ggagaggccg gacaccagga cgttcgcaat ggctcgatcc
     1981 gtctcggcgc ctgcgggcgg caggtcatcg aaacccgcca ccgagaagat gaccacgggt
     2041 ccctcgccct tatccggcca ccagccgtag atattgaggg tgtcgtcatc gcgcagccag
     2101 tgccgggtga cgcaggcgag cacgttcacg tgcaattcca gcggcacgcg gcccagccgg
     2161 ttcgcaagct tttcggcc
//
LOCUS       DATIRT010000426         4444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1646218, whole genome shotgun sequence.
ACCESSION   DATIRT010000426 DATIRT010000000
VERSION     DATIRT010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1646218"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1463)
                     /locus_tag="VLN25_09585"
     CDS             complement(<1..1463)
                     /locus_tag="VLN25_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003071670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HSQ10849.1"
                     /translation="MDHTHAHGDHAGCSHSHPEPARPLELKDPVCGMKVTEESAHRIE
                     HAGQPYYFCSAGCKAKFAADPMKYVAPEPATPAPDAVTPAGTIYTCPMHPEVRQDHPG
                     NCPKCGMALEPEMPSLEEDENPELTDFRRRFWWSLPLTVIVTVLGMFGHRLQWFEMAT
                     QSWIELLLTAPIVLWAGWPFFVRMAQSVRNRSPNMWTLIGLGTGAAFVYSLVATIAPQ
                     VFPQSFISMGRVAVYYEAAAVIISLTLLGQLLELKARSKTSAAIKALLGLAPKTARRI
                     RTDGSEEDVPLAHVHVGDTLRVRPGEKVPVDGIVIDGGSAVDESMLTGEPMPVYKRPD
                     DKLIGGTLNTSGALIMRTERVGSATVLAQIVQLVAQAQRSRAPMQRMADQVAGWFVLV
                     VVAIAVATFFGWGLFGPEPSWVYGLVNAVAVLIIACPCALGLATPMSIMVATGRAAQH
                     GVLFRDAAAIENLRKVDTLVVDKTGTLTEGKPVFESA"
     gene            1572..2042
                     /locus_tag="VLN25_09590"
     CDS             1572..2042
                     /locus_tag="VLN25_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HSQ10850.1"
                     /translation="MTTAQPATLTIGRLARAAGVGVETIRYYQRRGLLPVPGTSGAFR
                     HYPAALLGRIRFIKRSQELGFSLEEIGQLLRLEDGADRRSVRRISAERLEQIDAKLAD
                     LKRMQRVLGRLVAECEHADLNRPCPIIATLSAGEPMAEAQPSKSGRTRAARTDH"
     gene            complement(2045..2263)
                     /locus_tag="VLN25_09595"
     CDS             complement(2045..2263)
                     /locus_tag="VLN25_09595"
                     /inference="COORDINATES: protein motif:HMM:NF023036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HSQ10851.1"
                     /translation="MAEGEVRRVDRETRKITLRHGEIRQLDMPPMTMVFQVKDPALLD
                     MVKPGDKVKFRAEKIGSAYTITEIEAVR"
     gene            complement(2398..2838)
                     /locus_tag="VLN25_09600"
     CDS             complement(2398..2838)
                     /locus_tag="VLN25_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HSQ10852.1"
                     /translation="MLLSVLSPATLGQDTPLQIEVWKSLSCGCCQDWIDYLEKNGFSV
                     KVHESGNDAARRRLGLPGMYGSCHTGLIGGYVVEGHVPVREIRRLLKEKPDAVGIAVP
                     AMPVGSPGMDGPAFGKRFDPYDVILVARDGSARVYQTYRSKDDQ"
     gene            complement(2868..3371)
                     /locus_tag="VLN25_09605"
     CDS             complement(2868..3371)
                     /locus_tag="VLN25_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003077158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family protein"
                     /protein_id="HSQ10853.1"
                     /translation="MIILHTLARPAMLAGALALMPLGPAAAHGEKTHRKAPIVKEQKP
                     WGIAGDPKSAKRTVEFRMTDDMRFSPDRIQVTQGETVRFVLVNHGKVMHEFVLGTRQE
                     LEEHAALMQKFPDMEHDEPYMAHVAPGRTGTIVWTFNRAGEFDFACLIAGHFQAGMVG
                     KVSVSAL"
     gene            complement(3402..>4444)
                     /locus_tag="VLN25_09610"
     CDS             complement(3402..>4444)
                     /locus_tag="VLN25_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HSQ10854.1"
                     /translation="HTTIHWHGQRIPNGMDGVGGLNQKQIPPGKTFVYEFVARRPGTF
                     MYHPHADEMTQMAMGMMGFWVTHPRAKHPLIDDVDRDFCFLLNAYDIDPGSFTPKIMT
                     MLDFNLWSWNSRIFPGIDSLNVRLNDKVRIRIGNLTMTNHPIHLHGHEFLVTGTDGGP
                     TPKSTRWYEVTTDVAVGQMRQIEFVADEEGDWAFHCHKSHHTMNAMGHDVPTMIGVDH
                     RGLARKITSLIPDYMVMGERGMADMAEMEMALPDNTIPMMTGQGPYGSVEMGGMFTVV
                     KVRRNQKQGNYADPGWFQQPAGTQAYEWTGALAEPKRFRSEGSQSMSPQKAPATEIEV
                     KIRKPNGHAGHH"
BASE COUNT          787 a         1435 c         1355 g          867 t
ORIGIN      
        1 atcgcacttt cgaatacagg cttgccctct gtcagcgtcc cggtcttgtc gacgaccagc
       61 gtatcgacct tgcgcagatt ctcgatggcg gccgcgtcgc ggaacaggac gccatgctgg
      121 gcggcgcgcc cggtcgccac catgatggac atcggcgtcg ccagtcccag cgcacagggg
      181 caggcgatga tcagtaccgc gacggcgttg accagcccgt agacccagct cggttcgggg
      241 ccgaacagcc cccagccgaa gaaagtcgcg acagcaatcg ccaccacgac cagcacgaac
      301 cagccggcga cctgatcggc catgcgctgc atgggtgccc tcgaacgctg agcctgcgcc
      361 acgagttgaa cgatctgcgc gagcaccgtt gcgctgccga cgcgctccgt gcgcatgatc
      421 agcgcgccgc ttgtattcag cgtgccgccg atcagcttgt cgtccgggcg cttgtatacc
      481 ggcatcggct cgccggtgag catcgactca tccaccgcac tgccgccgtc aatgacgatg
      541 ccgtcgaccg gcaccttttc tcctggccga acgcgcaacg tatcgcccac atgcacatgc
      601 gccagcggta cgtcttcttc gctgccgtcg gtcctgatgc ggcgcgcggt tttcggggcg
      661 agtccgagca acgccttgat cgcggccgac gtcttcgagc gtgcctttag ttcgagcagt
      721 tgcccgagca gtgtcaacga aatgatgacg gcagcggctt cgtaatagac ggcgacacgt
      781 cccattgata tgaaggactg cgggaacacc tgcggcgcga tcgtcgccac gaggctgtag
      841 acgaacgcgg cgccggtacc cagcccgatc agcgtccaca tgttgggact gcgattcctg
      901 actgactgcg ccatgcgtac aaagaagggc cagcccgccc acagcacaat gggcgcggtc
      961 agcagcagct cgatccagct ctgcgtggcc atctcgaacc actgcagtcg atgtccgaac
     1021 atgccgagga cagtgacgat gactgtcagc ggcagcgacc accagaaacg gcggcgaaag
     1081 tccgtcagct cggggttctc gtcttcctcc aggcttggca tctccggctc gagtgccatg
     1141 ccacatttcg gacaattgcc cggatggtcc tgccgcactt cgggatgcat cgggcacgtg
     1201 tagatcgtgc ccgccggggt gactgcgtcg ggcgcgggcg tggctggctc cggtgcaacg
     1261 tacttcatcg gatccgctgc gaacttcgcc ttgcatccgg cactgcagaa gtaatagggc
     1321 tgtccagcat gctcgatccg gtgcgcagac tcctcggtca ccttcatgcc gcaaaccggg
     1381 tccttcagct cgagcggccg cgccggttcc ggatgtgaat gggaacagcc cgcgtgatca
     1441 ccgtgtgcat gtgtgtggtc catcgtgact cccgtcgaca aatcgatgtc cggagcttag
     1501 agtccgtacc cgaccccaga gtcaagctct atttcatcta ttaaatcgaa actcgaaagg
     1561 acagcgtccc gatgacgacc gcccaacctg caacgctgac cattggccgg ctggcacgtg
     1621 cggccggagt cggcgtcgag accatccgtt attaccagcg acgcggactt ctgccggttc
     1681 ccggaacgtc gggcgctttc cgtcactatc cggctgccct gctgggtcga attcgattta
     1741 tcaagcgttc gcaggaactc ggcttctcgc tcgaggagat cggacaactg ttgagactcg
     1801 aagacggcgc cgaccgccgc tcggtccggc ggatctcggc cgagcggctg gagcagatcg
     1861 acgccaagct cgccgacctc aagcgcatgc agcgcgtgct cgggcgcctg gtggccgagt
     1921 gcgagcacgc cgacctgaac cgcccttgcc cgatcatcgc cacgctgtcg gccggtgagc
     1981 cgatggcgga agcgcagccg tcgaaatcgg gtcgaacccg cgctgctcgc actgaccact
     2041 gaggtcatct aaccgcctcg atctcggtga tggtgtaggc cgaaccgatc ttttccgcgc
     2101 ggaacttgac cttgtcgccg ggcttgacca tgtcgagcag tgccgggtcc ttgacctgaa
     2161 aaaccatcgt catcggtggc atgtccagtt gcctgatctc accatgacgc agggtgatct
     2221 tgcgggtttc gcgatcgacg cggcggactt cgccttccgc catgtcatca gcagcctggg
     2281 acacgggcgc tgaagccgtg tgctcgttgc cactcgcact ctccggcatc gatatcgaag
     2341 tggcaccgag aagaagcgac actccaagcg tggcaattcg ggcttgcgcg cggagtctca
     2401 ttggtcatcc ttcgatcgat aggtctggta cacgcgggcc gatccatcgc gcgcaaccag
     2461 tatcacgtca tacgggtcga accgcttgcc gaaggccggg ccgtccatgc cgggtgagcc
     2521 gaccggcatc gccggcaccg cgatgccgac ggcgtccggc ttttccttca gcaggcggcg
     2581 gatctcgcgg acaggcacat gtccctccac cacgtagcca ccgatcagcc cggtgtggca
     2641 ggagccatac atgcccggca ggccaaggcg ccggcgtgcg gcgtcgttgc ccgactcgtg
     2701 gaccttgacc gagaacccgt tcttttcgag gtaatcgatc cagtcctggc agcagccgca
     2761 tgaaagcgac ttccagactt cgatctgcag gggagtgtcc tggccgaggg tcgccggtga
     2821 aagcaccgac agcagcatgg cggtacacca ccgacgccgg tccgggctca tagggcgctc
     2881 accgaaacct tgccgaccat cccggcctgg aagtgcccgg caatcaggca ggcaaagtcg
     2941 aactcacccg cccggttgaa ggtccagaca atggttccgg tcctgcctgg tgcgacgtgc
     3001 gccatgtaag gctcgtcatg ctccatgtcc ggaaacttct gcatcagcgc agcgtgttct
     3061 tcgagctcct ggcgcgtgcc gagaacgaac tcgtgcatca ccttgccgtg attgaccagg
     3121 acgaaccgaa ccgtctcccc ttgcgtgacc tggattcggt caggggagaa gcgcatgtcg
     3181 tcagtcatcc tgaattcaac tgtccgcttc gccgacttcg ggtcgccggc aatgccccag
     3241 ggcttctgtt ccttgacgat gggggctttc ctgtgcgtct tctcgccgtg tgccgcggcc
     3301 gggccgagcg gcatcagggc caacgcgccg gcaagcatcg ccggtcgggc cagcgtgtgc
     3361 agaatgatca tcatcgtgga tcctttttct ggagggtatg ttcagtggtg gcccgcatgg
     3421 ccgttcggct tacggatctt tacttcaatt tccgttgccg gtgctttctg gggggacatc
     3481 gactggctgc cttcggaccg gaagcgcttc ggctcggcca gcgctccggt ccattcgtag
     3541 gcttgagtgc cggcaggctg ctggaaccaa ccgggatcgg cgtagttgcc ctgcttctgg
     3601 ttccgccgca ccttgacgac cgtgaacatc cctcccatct caaccgagcc gtagggaccc
     3661 tgtccagtca tcatcggaat ggtgttgtcc ggcaaggcca tttccatttc cgccatatcg
     3721 gccatgccgc gctcgcccat caccatgtag tcgggtatca gcgaggtgat cttgcgcgcg
     3781 agcccgcgat gatccactcc gatcatcgtc ggcacatcgt gtcccatcgc gttcatcgtg
     3841 tggtgcgact tgtggcagtg gaacgcccag tcgccctcct catcggccac aaactcgatc
     3901 tgccgcatct ggccgactgc gacgtcggtc gtgacctcgt accagcgcgt ggacttcggc
     3961 gtcggtccac cgtcggtgcc ggtcaccaga aactcgtgac cgtgcagatg gatcggatga
     4021 ttcgtcatcg tcaggttgcc aatgcggata cgcaccttgt cgttcaaccg aacgttcagt
     4081 gagtcgatgc cgggaaagat gcggctgttc cacgaccaca ggttgaaatc gagcatcgtc
     4141 atgatcttcg gagtgaagct gccggggtcg atgtcgtaag cgttcagcag gaagcagaag
     4201 tcgcgatcga catcgtcgat caggggatgc ttcgcacgcg gatgggtgac ccagaatccc
     4261 atcatcccca tggccatctg cgtcatctca tcggcgtggg ggtggtacat gaaggtgccg
     4321 ggacgtcgcg cgacaaactc gtagacaaag gtcttgcccg gaggaatctg cttctggttc
     4381 agtcccccca cgccgtccat gccgttcggt atccgctggc cgtgccagtg gatggtggtg
     4441 tgct
//
LOCUS       DATIRT010000427         3384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1351078, whole genome shotgun sequence.
ACCESSION   DATIRT010000427 DATIRT010000000
VERSION     DATIRT010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351078"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1663
                     /gene="aceE"
                     /locus_tag="VLN25_09615"
                     /pseudo
     CDS             1..1663
                     /gene="aceE"
                     /locus_tag="VLN25_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141660.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; internal stop; incomplete;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
     gene            1763..>3384
                     /gene="aceF"
                     /locus_tag="VLN25_09620"
     CDS             1763..>3384
                     /gene="aceF"
                     /locus_tag="VLN25_09620"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HSQ10855.1"
                     /translation="MGQLVEVQVPDIGDFKEVEVIEVLVKPGDAVAKEQSLITVESDK
                     ASMEIPSSAAGMVKELRVKLGDKVAQGSLVLVLEAGDGAAAPTPAPPPAAKAPPPKAA
                     AAPPPATAAAAVPTSAPAPAAGPIEVVVPDIGDFKEVEVIEILVKPGDSVKQEQSLIT
                     VESDKASMEIPSSAAGVVQELRVKIGDKVSKGTPVVVLTGTAAAPASPSSAAAAPVAA
                     VASSTAPVEPRSTAFATEMGARPPPVDLGPAPGAKPHASPSVRKFARELGVDLTHVRG
                     SGPKGRITTDDVRDHVKGVMASGARPVAAPAGTGVGLNLPAWPVVDFAKFGPIERVEM
                     SRIRRISGPALSRNWVTIPHVTNQEDADITDLEAFRVQLNKENEKSGLKLTMLAFLIK
                     ACVAALKKFPDFNSSIDPEGGAIIRKNYWHIGFAADTPQGLVVPVVKDADKKGVFEIA
                     QETSALAAKARDGKLGPSDMQGGTFSISSLGGIGGTSFTPIINAPEGAILGVCRSTMK
                     PVWDGKQFVPRLILPLSLSWDHRVVEGAGAAR"
BASE COUNT          625 a         1080 c         1141 g          538 t
ORIGIN      
        1 gctgctcgaa atggtgtcgc gcatgaccga cgacgacatc tggcgactca atcgtggcgg
       61 gcacgacccg cacaagatct acgccgcctt tgcggccgcg atgaagacca ccggacaacc
      121 gacggtgatt ctcgccaaga ctgtcaaggg cttcggcatg ggcaaggtcg gagagggcaa
      181 gaatccgacg caccagctga agaaactcga taccgcggcc atccgggagt tccgcgaccg
      241 ctttgcaatc ccggtggccg acgaccagct ggaagctgtc ccgttcttca agcccgacgc
      301 gaattcgccg gagatgaagt acctgatgga ccggcgccgc gcgctgggcg gtttcgtgcc
      361 gcagcgccgg cgcacggcgg acgaaaaact cgccgccccg acgctggatg cgttcgcccc
      421 ggtgctcgag gcgaccgctg aaggacgcga gatctccacc acgcaggcct tcgtgcgctg
      481 cctcacgcag ctggtgcgcg acaagagcgt cggtccgcga gtcgtgccga tcattccgga
      541 cgaggcccgc acgttcggca tggagggcat gttccgccag ctcgggatct attcgtcgca
      601 gggccagctc tacgagccgg tcgaccgcga ccaggtgatg tactaccgcg aggacaagca
      661 gggccagatc ctcgaagagg gcatcaacga ggccggtgcc atgagttcat ggatcgcggc
      721 ggcgacgtcg tattagacga acaaccgcat catgctgccg ttttacatct tctattcgat
      781 gttcggcttc cagcggatcg gcgacctcgc ctgggccgcc ggcgacatgc aggcgcgcgg
      841 tttcctgctc ggcgcgaccg ccgggcgtac gacgctgaac ggcgagggac tgcagcacca
      901 ggacgggcac tcgcacctga tgagtgcgac gattccgaac tgcatcagct atgaccccac
      961 ctacgcgcac gaagtggcgg tgatcatcca gcacggcctc aggcgcatgg tgcaggacca
     1021 ggaaaacttc tggtactaca tcacgttgat gaacgagaac tacccgcatc cgggcctgaa
     1081 gaaaggccag gaggagggca tcctgcgcgg catgtacctg ctgcgcgagg gacccgccgg
     1141 cagcggcatg ggcaagtcga ttgcgcgtgt gcagttgctc ggcagcggaa ccatcctgcg
     1201 cgaggccatc gccgcggccg aaatgctcga gaaggactgg ggcgtgtccg gagatgtgtg
     1261 gagcgtcacc agcttcacgg aactgcgccg tgacggcatc gatgtcgagc gctggaacct
     1321 gctgcatccg acggacaagg tgcgcaagcc ctacgtgacc cagctgcttg aacgcaccgc
     1381 cggaccaatc gttgcaacga cggactacat gcgcctgttc gccgaccaga tccgccctta
     1441 cattccccgc gggcgtgact accgcgtgtt cggcaccgac ggcttcgggc gctccgatac
     1501 acgcacgaag ctgcgcgagt tcttcgaggt cgatcgtcgc tacgtcgtga tcgcggcgct
     1561 gcgatcgctt gccgacgagg gcatcgtgcc gatcgccaag gtggcagagg cgatcaagaa
     1621 gtatgggatc gatccggcta agcgcaatcc cgtgacggtc tgatcgctgt ccgcgcgtga
     1681 accatcgcgt gcgggccgtg agcctctgac gatccgcgct gccccgcagc gcaaggacac
     1741 catcaacgaa agggccaccg acatgggcca gctcgtggaa gttcaggtcc ccgacatcgg
     1801 cgacttcaag gaggtcgagg tgatcgaggt gctggtcaag cccggagatg cggtggcgaa
     1861 ggagcagtcg ctgatcacgg tcgagagcga caaggcctcg atggagatcc cgtcgtccgc
     1921 cgctggcatg gtcaaggagt tgcgggtgaa gctcggtgac aaggtcgcgc agggcagcct
     1981 cgtgctcgtc ctcgaagctg gcgacggagc ggctgcgccg acaccggcac cgcctcccgc
     2041 ggccaaggca cctccgccga aagcagctgc ggcacctccg ccggcgacgg ctgccgctgc
     2101 tgtccccaca tctgcccctg cgccggccgc cggaccgatc gaggttgtcg tgcccgacat
     2161 cggtgacttc aaggaagtcg aggtgatcga gatcctggtc aagcctggcg actcggtgaa
     2221 gcaggagcag tcgctgatca cggtcgagag cgacaaggcc tcgatggaga ttccgtcgtc
     2281 ggccgcgggt gtcgtccagg aactgcgcgt gaagattggc gacaaggtgt cgaagggcac
     2341 gccggtcgtc gtgctcacgg gtactgcggc cgcgccagcg tcaccgtcgt ccgcagcggc
     2401 tgcgccggtc gcagcggtcg cgtcgtcgac cgcaccggtc gagccgaggt cgactgcgtt
     2461 cgccaccgaa atgggcgcgc ggccaccacc ggtcgatctc gggccggcac ccggcgccaa
     2521 gccgcacgcg tcgccgtcgg tgcgcaagtt cgcgcgcgag ctcggtgttg atctcaccca
     2581 cgtgcgcggc agcgggccca agggccgtat cacgaccgat gatgtccggg accacgtcaa
     2641 aggcgtgatg gcgagcggtg cccggccggt cgctgcgcca gcgggcacgg gagtcggact
     2701 caacctgccg gcgtggccgg tcgtggattt cgccaagttc ggtccgatcg aacgggtcga
     2761 gatgtcgcgc atccgccgga tttccggtcc ggcgctgtcg cgcaactggg tgacgattcc
     2821 ccacgtcacc aaccaggaag acgccgatat caccgacctt gaggcgttcc gtgtccagct
     2881 gaacaaggaa aacgagaaga gcggactcaa gctgacgatg ctcgcgttcc tgatcaaggc
     2941 ctgcgtggcc gcgctgaaga agttcccgga cttcaacagc tcgatcgacc ccgagggcgg
     3001 cgcgatcatc cgcaagaatt actggcacat tggcttcgcg gccgacacgc cgcagggtct
     3061 ggtggtgccg gtcgtgaagg atgccgacaa gaagggcgta ttcgaaattg cacaggaaac
     3121 gtcggcgctc gcggccaagg cgcgcgacgg caagctcgga ccatccgaca tgcagggcgg
     3181 cacgttctcg atctcctcgc tcggcgggat cggcggtacg tcgttcacgc cgatcatcaa
     3241 cgcaccggaa ggggcgatcc tcggggtgtg ccggtcgacg atgaagccgg tgtgggacgg
     3301 caagcagttc gtgccccgac ttattctgcc gctgtcactg tcgtgggatc accgcgtcgt
     3361 cgaaggagcg ggtgcggcgc gctt
//
LOCUS       DATIRT010000428         5022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_563833, whole genome shotgun sequence.
ACCESSION   DATIRT010000428 DATIRT010000000
VERSION     DATIRT010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5022
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563833"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VLN25_09625"
     CDS             <1..397
                     /locus_tag="VLN25_09625"
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSQ10856.1"
                     /translation="PPDQVTGVIVEATFTSIPDLIRGMRWGWVPGIGLVVTQRFDSSE
                     RIEKVKAPLLILHGTADNVVPHTMADELYERAGSRDKRIVKIEGGTHSGSRGGTSTYR
                     EAVLSFVQSVAPASASAMGASAGAASAAD"
     gene            604..3084
                     /locus_tag="VLN25_09630"
     CDS             604..3084
                     /locus_tag="VLN25_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HSQ10857.1"
                     /translation="MDQSNATVALAGGAPAPAAERLVLPVQGMTCASCVAHVEKALAR
                     VAGVERVAVNLATESAAVEGHTLDPQALARAIDAAGYTVPTQTIRLAIEGMTCATCVA
                     RLETALRSVAGVTKVGVNLASDSASVEIVSGSATARDLVAAVVAAGYSATLPADSGPA
                     VPIDRTRGLRRDALLALALALPLLIGSHLTLVGIEWAMPAWMQWLLATPVQFWCARRF
                     YFAAAKAVRAGTGNMDLLVSLGTLAAYGLSLYLWLEGGASRATHGPVHLYFEAAAVVI
                     ALVLLGKWLEARAKHQTSEAIRLLAALRPTTANVLRDQHIVEVPVEDLRLGDIVVVRG
                     GDRIPVDGLIRSGSSSLDESMLTGESLPVDKSPGERVSAGSINGPGRLDVETRAVGAE
                     TMLARIIRLVEDAQSAKAPIQRLVDRVATFFVPVVLVIAAATVAGWLAAGAPLEIALI
                     NAVSVLVIACPCALGLATPAAIIAGTGVAARHGILIKDAQALELAKRIDVVAFDKTGT
                     LTIGKPRVVAVEDHQLFDPKEVLALAEAANAGSNHPLAESVREAFEHEAGRHPEMVRR
                     KAIEHRVLPGRGVASRLVDAAHPDTSIELAFGNPRLMQEWQVDTRVLDARALHFESEG
                     RTVSWLAQRTPAGPQLIGLVAFGDEPRPSARAAIEELERLGIETTMISGDNDGAARHV
                     AKQLGLSRYEANVLPRDKAKRVEALRKDGRVVAMVGDGINDAPALAAADVGIAMGSGT
                     DIAMHAAAVTLLRSDPRLVATAIEISRATVRKIRQNLFWAFIYNIVGIPLAAFGVLTP
                     VFAGAAMALSSVSVVSNALLLKRYRSGK"
     gene            complement(3148..3942)
                     /locus_tag="VLN25_09635"
     CDS             complement(3148..3942)
                     /locus_tag="VLN25_09635"
                     /inference="COORDINATES: protein motif:HMM:NF017672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006672 - ceramide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ceramidase domain-containing protein"
                     /protein_id="HSQ10858.1"
                     /translation="MTRTADGASNRTASVIVLMALCAAVAPWLVAWVLPPMAQPQTYH
                     DFADQRTLWGIPHALNVLSNLPFVVVGLMGLQALATRVRFVSRDRGARAPWIVLFIGV
                     ALTGLGSAYYHLDPNDATLVWDRLPMALAFAGLVAGTLADRAVRLAWMLLALLGGVGT
                     GAVIYWAVLGNLIPYLVMQAGFIAIAVAATVMIRSPYSHAGVLYAAVALYAAAIVSER
                     LDHPINAALGSVVSGHTLKHLFAAAGGYAIYRMLVMRQPAAAGPSR"
     gene            complement(3939..4259)
                     /locus_tag="VLN25_09640"
     CDS             complement(3939..4259)
                     /locus_tag="VLN25_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10859.1"
                     /translation="MSRAWIVDAVDTAGIDPRERQRVIDDLLSFEPPRRNGVRNTFIF
                     WAGVIGAAMLADAIGLPGWIGFTFALACFAWIGRELAVRALRWRLDQWRQPNAGEPQV
                     KARE"
     gene            complement(4308..>5022)
                     /locus_tag="VLN25_09645"
     CDS             complement(4308..>5022)
                     /locus_tag="VLN25_09645"
                     /inference="COORDINATES: protein motif:HMM:NF044969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HSQ10860.1"
                     /translation="RAYLLTREVFDLRPTWKAIEALDNVVSDEMQAELLMELGRRTVR
                     ATTWFLRSRRLADPIGQTIERFAPAARAMLKSLQAAPPSAAWRAPILEMREGYVRQGV
                     PAPLADEVAAAGTLLAALDLAEVAEASRQPLDTVATAYFSIGESLGLTRLRTQVSGLP
                     SDGYWQALAKAATNDDLASLQRQLTADALAAGGLEAWQTAQAAALDRARRLLGELAES
                     KSADLAMLSVALRELRNLA"
BASE COUNT          838 a         1729 c         1678 g          777 t
ORIGIN      
        1 gccgccggat caggtgaccg gcgtgatcgt cgaagcaacc ttcacgtcga tccccgacct
       61 gatacgtggc atgcggtggg gctgggtgcc cggcatcggc ctggtcgtga cccagcgctt
      121 cgactcctcg gagcgcatcg agaaggtgaa ggcaccgctg ctgatcctgc acgggacggc
      181 cgacaacgtt gttccccaca cgatggccga cgagctctac gagcgcgcgg gcagccgcga
      241 caaacgtatc gtcaagatcg agggcggcac ccattcaggg tcccgtggtg gcacctcgac
      301 ctaccgcgag gccgtgctga gcttcgtgca atcggttgct ccggcctcgg cctcggcgat
      361 gggcgcgtcg gccggcgccg cgtccgcggc cgactgacgc cgctgccgca aaggcagccg
      421 ctttctctga agttacagcg ggttcgaaaa agcgcgatac cccagttcgc ggacaagtat
      481 tgcattggaa cgctttgccc aaatcgtggg aacaatgcgt tgaaaagcgc acgtcttgac
      541 tccgctcaac gtgtctcggg ccgaaccgat cgtaactagc ggcagggcgt agagtttcga
      601 gcaatggatc aatccaacgc caccgttgca ctggccggag gcgcccccgc cccggcggcc
      661 gagcggctcg tactgccggt acagggcatg acctgcgcga gttgtgtcgc gcatgtcgaa
      721 aaggcgctcg ccagagtggc cggggtggaa cgggttgccg tcaacctcgc gaccgagtcg
      781 gccgcggtcg agggccacac gctcgatccg caggcgcttg cgcgagcgat cgatgccgcc
      841 ggctataccg tgccgaccca gacgatccgg ctggccatcg agggcatgac ctgcgccacc
      901 tgcgtcgctc gcctcgaaac cgcactgcgc agcgttgcgg gcgtgaccaa ggtcggcgtc
      961 aacctcgcct ccgattcggc cagcgtcgag atcgtgtcag gctccgcgac ggcgcgcgat
     1021 ctcgtcgcgg cagtcgtcgc ggccggctac tccgcgactc tccccgcaga cagtggcccc
     1081 gccgtaccga tcgaccgcac tcggggactt cggcgtgacg cgctgcttgc gctcgcactg
     1141 gcactgccgc tgctgatcgg atcgcacctc acactggtcg gcatcgagtg ggcgatgcca
     1201 gcatggatgc agtggctgct cgccacgccg gtgcagttct ggtgcgcgcg ccgcttttac
     1261 ttcgcggccg ccaaggctgt gcgggccggc accggcaaca tggacctact ggtgtcgctg
     1321 ggtacgctgg ccgcctacgg cctttccctc tatctgtggc tcgaaggcgg cgccagccgt
     1381 gcgacacacg gcccggtgca tctgtacttc gaagctgccg ccgtcgtcat tgcactggtg
     1441 ctgctcggca agtggctgga ggcgcgcgcc aagcaccaga cttccgaggc gatccgtctg
     1501 ctggccgcat tgcgaccgac gacggcgaac gtgctgcgtg accagcacat cgtcgaagtg
     1561 ccggtcgagg atctgcgact gggcgacatc gtcgtcgtgc gcggcggtga ccggatcccg
     1621 gtcgacggcc tgatccgttc cggttcgtcg agcctggacg agtcgatgct gaccggcgag
     1681 agcctgccgg ttgacaaatc accgggtgaa cgcgtcagcg cgggctccat caacgggcca
     1741 ggacggctcg acgtcgagac tcgtgccgtc ggcgccgaga ccatgctcgc ccggatcatc
     1801 cggctggtcg aggatgcgca gtcggccaag gcgccgatcc agcggctggt cgatcgcgtt
     1861 gccactttct tcgttccggt cgtgctggtc atcgcggccg ccacggtcgc gggttggctc
     1921 gccgccggcg cgccgctcga aatcgcgctg atcaatgccg tctccgtgct tgtcatcgcc
     1981 tgtccgtgtg cgctcggact ggccacgccg gcagcgatca tcgccggtac cggtgtcgcc
     2041 gcccgccacg gcatcctgat caaggacgcg caggcactcg agctcgccaa gcgcatcgat
     2101 gtggtcgcgt tcgataagac cggcacgctc acgatcggca agccccgcgt agtcgcggtc
     2161 gaggaccatc agctgttcga tcccaaggaa gtgctcgcgc tggccgaagc ggccaatgcc
     2221 ggcagcaatc atccgttggc cgagtcggtg cgcgaggcgt tcgaacacga agccgggcgt
     2281 cacccggaga tggtccgccg caaggccatc gagcatcgtg tgctgccggg ccgcggcgtg
     2341 gcaagtcgac tcgtcgatgc agcccatccc gacacaagca tcgaactcgc cttcggcaac
     2401 ccgcggctga tgcaggagtg gcaggtcgat acgcgcgtgc tcgatgcgcg ggcgctccac
     2461 ttcgagagcg aagggcgcac cgtatcgtgg ctggcgcagc gcacgcccgc cggaccgcag
     2521 ctgatcggac tggtcgcctt cggcgatgag ccgaggccga gcgcgcgcgc cgccatcgag
     2581 gaactcgagc ggctcggcat cgaaacgaca atgatctcgg gtgacaacga cggcgcggca
     2641 cggcacgtgg cgaagcaact cggcctcagc cgctacgagg ccaacgtcct gccgcgcgac
     2701 aaggccaaac gagtcgaggc actgcgaaag gacgggcgcg tcgtcgcgat ggtgggcgat
     2761 ggcatcaacg atgcgccggc cctggcagcg gcggacgtcg gcattgcaat gggttcaggc
     2821 accgacatcg ccatgcatgc ggccgcggta acgctgttgc gctccgaccc gcggctggtg
     2881 gccaccgcga tcgaaatatc gcgcgcgacc gtgcgaaaga tccgccagaa cctgttctgg
     2941 gccttcatct acaacatcgt cggcattccg ctggctgcgt tcggtgtgct gacgccggtg
     3001 ttcgccgggg ccgccatggc gctgtccagc gtctcggtcg tcagcaatgc gctgcttctg
     3061 aagcgctacc gatcgggaaa gtgagtgcgg cccgcgtacg ctggcgtcgc gcctgagctt
     3121 gcctgccgta acagcgtgag aacggcgtca gcgcgacggc cccgccgcag cgggctggcg
     3181 catcaccagc atccggtaga tcgcgtagcc accggcggcg gcaaacagat gcttgagcgt
     3241 atgcccgctg accacgctgc cgagcgccgc attgatcggg tgatccaggc gttcgctgac
     3301 gatggccgcg gcatacagcg ccaccgcggc gtagagaact ccggcatgcg agtacggcga
     3361 ccggatcatc acggtcgccg cgaccgcgat cgcgatgaag cccgcctgca tcacgaggta
     3421 gggaatgaga ttgccaagca cggcccagta gatcacggcg cccgtaccga cgccgcccag
     3481 tagcgccaga agcatccacg cgagtcgcac ggcgcgatcc gcgagtgtgc cggcgacgag
     3541 gccggcgaag gccagcgcca tcggcagccg atcccacacc agcgtcgcat cgttcggatc
     3601 gaggtggtag taggcggagc ccaggccggt cagcgccacg ccgatgaaca acacgatcca
     3661 gggtgcgcgc gcgccgcgat ccctcgaaac gaatcgaaca cgggtggcga gcgcctgcaa
     3721 tcccatcagg ccaacgacca cgaacggcag gttggaaagc acgttcaacg cgtgcgggat
     3781 tccccagagc gtccgctggt ccgcgaaatc atggtaggtc tgcggctgtg ccatcggtgg
     3841 cagcacccag gccaccagcc acggcgccac cgccgcgcac aacgccatca ggacgatgac
     3901 gctggccgtc cggttcgatg ccccgtccgc cgtacgcgtc attcccgagc cttcacttgg
     3961 ggttccccgg cattcggctg tcgccactgg tcgagtcgcc accgtagcgc gcgcaccgcc
     4021 agttcccggc cgatccacgc aaagcaggcg agcgcgaacg taaagccgat ccagcccggc
     4081 agcccgatcg catcggcgag cattgcagca ccgatgacgc cggcccagaa aatgaacgtg
     4141 ttgcgcacgc cgttgcgccg tggcggctcg aagctcaaca ggtcatcgat cacgcgctgt
     4201 cgctcgcgcg gatcaatgcc tgcggtgtca acggcatcga cgatccacgc gcgcgacacc
     4261 aggcccgccg cggcgggagt caaccggtcc tgccagcggc gctgcactca ggcgaggttg
     4321 cgcaactcgc gcaacgcaac cgacagcatc gccaggtcgg cgctcttgct ttcggccagt
     4381 tcgccgagca ggcgacgtgc ccgatcgagc gcggccgcct gcgcggtctg ccacgcctcc
     4441 agcccgcccg cggccagcgc atcggcggtg agctgccgct gcaggctggc gagatcgtcg
     4501 ttggtggccg ccttcgccag cgcctgccag tagccgtcgg acggcaagcc ggacacctgc
     4561 gtgcgtagcc gcgtgaggcc gagcgattcg ccgatggaaa aataggctgt cgccaccgtg
     4621 tcgagcggct gacgtgacgc ttcggccact tctgccagat cgagtgccgc gagcaaggta
     4681 ccggcggccg ccacctcatc ggcgagcggc gccggcacgc cctggcgcac gtagccctcg
     4741 cgcatctcaa ggatcggtgc gcgccacgcg gcgctcgggg gtgcagcctg cagagacttc
     4801 aacatcgcgc gagcggccgg cgcgaagcgc tcgatcgtct gcccgatcgg gtctgccagt
     4861 cggcgtgacc gaaggaacca tgtcgtcgca cgcacggtgc gtcgtcccag ctccatcagc
     4921 agttcggcct gcatctcgtc gctgaccacg ttgtcgagcg cctcgatcgc cttccacgtc
     4981 ggccgcaggt caaagacctc gcgtgtcagc agataggcgc gc
//
LOCUS       DATIRT010000429         5813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_71994, whole genome shotgun sequence.
ACCESSION   DATIRT010000429 DATIRT010000000
VERSION     DATIRT010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5813
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71994"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VLN25_09650"
     CDS             complement(<1..196)
                     /locus_tag="VLN25_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HSQ10861.1"
                     /translation="MTVDRWIHIFAGTFILLSLALGASASPLFVSPWWLDFTAFVGAN
                     LFQYGFTNFCPLGWILKKLGV"
     gene            complement(207..3518)
                     /locus_tag="VLN25_09655"
     CDS             complement(207..3518)
                     /locus_tag="VLN25_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSQ10862.1"
                     /translation="MKDAGSSLGISGRIARSFLQSQLTPLIALVLLLLGAFAVMVTPK
                     EEEPQINVTMANVIIPFPGASSDDVHNMVTVPAEQVLSQITGIEHIFSVTQPGMAVLT
                     VQYKVGEPRIDSLVKLYDTLMSNGDWLPTHLGALAPIVKPKGIDDVPIVTLTLHTQRA
                     DGTADDLQRVAHELEIELKRVPGTREVSTLGSPGNVVRVTVDPERMNAHHVTVTDIER
                     ALRATNSSLPAGQLTSGNRTVEVRAGQFLRSAADVGQLVVGLAGPTRERRPILLADVA
                     RVTDCPDTPSLYVWHGAPASAASGTAREFVEWPAVTIQVSKKPGENAATVADAVMERV
                     AQLKGHLIPADVVVAETRNYGATASDKANQLIKKLIFATLSVVALVWATLGRREAIIV
                     GAAVVLTLASTLFASWAWGFTLNRVSLFALIFSIGILVDDAIVVVENIHRRSMIDHLP
                     LSQVIPRAVDEVGGPTILATFTVIAALLPMAFVSGLMGPYMAPIPINSSMGMLLSLAI
                     AFVITPWMALRLLPAAHSTPTGGDKLGARLASFFERVMRPFLRGDAGRGRRRWLGFGI
                     AVAIALSVLLVPLNLVVLKMLPFDNKSEFQIVVDMPTGSPVEATAALLRDLAVEVAKV
                     PEVVDYQAYAGLAAPINFNGLVRQYYLRSGGEVGDLQVNLVDKNRRDRKSHEIAQSVR
                     PALDAIARRHGADLRVVEVPPGPPVLSPIVAEIYGPDIDAQMKLAKQVRGVFERTPDI
                     TSIDDTVLDPAERIVLQVDTAKAAQYGIPANEVARIARMALAGEDVTPLHDGSSKYAV
                     PVRIGLADAAKGSMEALLKLKVRGPDTVRDEQTGLPYEIPLAELVRPTTLPREHVLHR
                     KDLQPVVYVTGDVAGGKGKTDSPLYGMFAMRADIAKISAPGGLPLAERWITAPVDRYA
                     SFAIKWDGEWQITYETFRDMGIAYAVGLVLIYLLVVAQFRSYLTPLIIMAPIPLTIIG
                     VLPGHALFGAQFTATSMIGMIALAGIIVRNSILLVDFIHQRLAEGIDFEQATIEAAAT
                     RAKPIVLTAVAAMLGAVFILDDPIFNGLAISLLFGIFVSTALTLVLIPVLYYAVNYRR
                     PKPAPVEPTPDPATA"
     gene            complement(3526..4608)
                     /locus_tag="VLN25_09660"
     CDS             complement(3526..4608)
                     /locus_tag="VLN25_09660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSQ10863.1"
                     /translation="MCSHRHATVPNRPRLAGRVALALLLLLSSSAVLADNGTPSVTTA
                     ATPAATVEVRMKDVEEVYAADAVIEAVRQATVAAQLSGTVTQIFVDAGDRVKKGQVLA
                     RIDTRDTDAQVAAGRASVAQADAQLTQARLNLERTQNLLEKNFVSRAALDQAESNFKA
                     AQAAADSARAGNTQANTARSHAELKSPIDGVITRRLMEPGEVAAPGRGVVAVHDPSAL
                     RAVGNLPQFVLPKAAGATRATVELPQLGRTLIATRVTILPATDARLLSTQVRADLPVG
                     DTPGVTPGAAAKILIPIGRTRKLVVPEAVLIRRGELTAVNVVTTDGRPQLRQVRVGSS
                     VAGGGIEVLAGLGDGERVLLNPLAVR"
     gene            complement(4629..4817)
                     /locus_tag="VLN25_09665"
     CDS             complement(4629..4817)
                     /locus_tag="VLN25_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HSQ10864.1"
                     /translation="MTANVGNLDRILRIVGGIALIALAATGTIGVWGYVGVVPLLTGL
                     MRSCPAYSLFGINTCRTT"
     gene            5137..5484
                     /locus_tag="VLN25_09670"
     CDS             5137..5484
                     /locus_tag="VLN25_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HSQ10865.1"
                     /translation="MEPELERLFEVVSGYFGLLAEPTRLKILNTICDGERSVSDIVDR
                     VGSSQTNVSRHLNLMYGRGVLKRRRDGAMVYYSISDPNVVTLCRTACVHIAGQQDDHS
                     VTSRTLKRFMPAK"
     gene            5530..>5813
                     /locus_tag="VLN25_09675"
     CDS             5530..>5813
                     /locus_tag="VLN25_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HSQ10866.1"
                     /translation="MSKGERAVAPGRIVAAPESHLTAEMLRGEARSRRSFLQGGAALA
                     GAVAAVAVGRAGAQEKAAFEPPNVPSWTRSLGAPVVAKPYGTPSEFEANV"
BASE COUNT         1035 a         1826 c         1964 g          988 t
ORIGIN      
        1 ggacacccag cttcttcagt atccagccga gcgggcagaa gttggtgaag ccatattgaa
       61 acagatttgc gcccacaaaa gctgtaaagt cgagccacca cggcgacacg aacagcggac
      121 tcgccgatgc gcccagcgcc agcgaaagca ggatgaaggt ccccgcgaag atgtggatcc
      181 agcgatcgac agtcatgagc gcctcctcag gcagtggcag ggtcaggggt gggttcaacc
      241 ggggccggct tgggcctgcg gtagttgacg gcgtagtaca ggacgggaat gagcaccagc
      301 gtgagcgcgg tcgaaacgaa aatgccgaac agcagcgaga tggcaagtcc gttgaaaatc
      361 ggatcgtcca ggatgaaaac ggcaccgagc atcgctgcca cggcggtcag cacgatcggc
      421 ttggcacggg tggcggcggc ctcgatcgtg gcctgctcga aatcgatgcc ttcggccagc
      481 cgctgatgga tgaaatccac cagcaggatc gagttgcgca caatgatccc ggcaagcgcg
      541 atcattccga tcatcgaagt cgcagtgaac tgcgcgccga acagtgcatg acccggcagc
      601 accccgatga tggtcagcgg aatcggcgcc atgatgatca gtggcgtcag gtaggagcgg
      661 aactgcgcga cgaccagcag gtagatcagt accagcccga cggcataggc gatgcccatg
      721 tcgcggaacg tttcgtaggt gatctgccat tcgccgtccc acttgattgc gaatgacgcg
      781 tagcgatcca ccggcgcggt gatccagcgt tccgccaggg gcaatccacc cggcgcactg
      841 atctttgcga tgtccgcgcg catggcaaac atcccatata gcggcgagtc ggttttgcct
      901 ttgccccccg ctacgtcccc cgtgacatag acgacgggtt gcaggtcctt gcgatgaagc
      961 acgtgctcac gaggcaacgt ggtcggccgg acgagctcgg cgagcgggat ctcatagggc
     1021 aggccggtct gctcgtcgcg cacggtgtcc gggccgcgga ccttgagctt cagcagcgcc
     1081 tccatcgaac ccttggccgc atcagccaat ccgatgcgca ccggcaccgc atacttgctc
     1141 gatccatcgt gcaacggcgt cacgtcttcg cccgcgagcg ccatgcgtgc gatgcgcgcc
     1201 acctcgttcg ccgggattcc gtactgggcc gccttggccg tgtccacctg aaggacgatg
     1261 cgttcggcgg ggtcgagaac ggtgtcgtcg atggaggtga tgtcgggcgt ccgctcgaat
     1321 acgccgcgga cctgcttggc gagcttcatc tgcgcatcga tgtcggggcc atagatctcg
     1381 gccacgatcg gcgacagcac cgggggtccc ggcggcactt cgaccaccct gaggtcggcc
     1441 ccatggcgac gggcgatcgc gtcgagcgcc gggcggacgg actgcgctat ctcgtggctt
     1501 ttgcggtcgc gtcggttctt gtcgacgagg ttgacctgga gatcaccgac ctcgccgccg
     1561 ctgcgaaggt agtactgccg cacgagcccg ttgaagttga tcggcgccgc cagtccggcg
     1621 taggcctggt agtcgaccac ttcgggcacc ttggcgactt ccaccgccag atcgcgcagc
     1681 agtgccgcgg tcgcttcgac cggtgacccc gtcggcatgt cgacaacgat ctggaattcc
     1741 gacttgttgt cgaacggcag catcttcagc acgacgagat tcagcggcac cagcagcacc
     1801 gatagcgcaa ttgccacggc gattccgaaa ccgagccagc ggcggcgacc gcggccggca
     1861 tcgccacgca ggaacggccg catcacgcgt tcgaaaaaac tggcgagccg ggcgcccagc
     1921 ttgtcgccac cggtaggcgt gctgtgggcc gccggcagca ggcgcaatgc catccagggc
     1981 gtgatcacga aggcgatcgc cagtgacagc agcatgccca tggacgagtt gatcgggatc
     2041 ggcgccatgt acggccccat caggccggag acgaaagcca tgggcaacaa ggcggcgatc
     2101 acggtgaagg tggccagtat cgtcgggccg cccacttcgt cgaccgcacg cgggatgacc
     2161 tgcgaaagcg gcaggtgatc gatcatcgag cggcggtgga tattctcgac gaccacgatc
     2221 gcatcgtcga ccaggatgcc gatcgagaag atcagcgcaa acagcgacac gcggttgagc
     2281 gtgaatcccc acgcccacga ggcgaagagc gtgctcgcca gcgtcagcac cacggccgcg
     2341 ccgacgatga tggcttcgcg ccggccgagc gtggcccaca ccagcgccac caccgacaac
     2401 gtggcgaaga tcagcttctt aatgagctga ttggccttgt cactggccgt cgcgccgtag
     2461 ttgcgcgttt ctgcgacgac gacgtccgcg gggatcaaat gccccttgag ctgggcaacg
     2521 cgctccatca cggcgtccgc gaccgtggcc gcgttctcgc cgggcttctt cgacacctgg
     2581 atcgtgacgg ctggccactc gacgaattca cgtgcggtgc cactggcagc ggacgcgggt
     2641 gcgccgtgcc agacgtacag gctgggagtg tccgggcagt cggtcacgcg tgccacgtcc
     2701 gccagcagga tcgggcgccg ttcgcgcgtc ggcccggcga gtccgaccac gagctgaccg
     2761 acgtccgccg cgctgcgcag gaactgtccg gcgcgcactt ccacggtccg gttgcccgag
     2821 gtcaactgcc cggccggcag cgacgagttg gtggcgcgca gcgcgcgctc gatgtcggtg
     2881 acggtgacat ggtgcgcgtt catgcgctcc gggtcgaccg tcacccgcac cacgttgccg
     2941 ggactgccaa gggtggacac ttcgcgcgtg cccggtacgc gcttgagctc gatctccaac
     3001 tcatgcgcga cgcgctgcag gtcatccgcg gtaccgtccg cgcgctgcgt atgcagtgtc
     3061 agcgtgacga tcggtacgtc atcgatgccc ttgggcttga caatgggagc gagcgcgccg
     3121 aggtgggtcg gcagccagtc gccatttgac atcagggtgt cgtacagctt gacgagcgag
     3181 tcaatacgcg gttcgccgac cttgtactgc accgtcagca ccgccatgcc cggctgggtg
     3241 acggagaaaa tgtgctcgat gccggtgatc tgcgacagca cctgctcggc cggcaccgtc
     3301 accatgttgt gcacgtcgtc gctcgaggcg cccggaaacg ggatgatgac gttcgccatc
     3361 gtcacgttga tctgcggctc ttcttccttc ggggtcacca tcacggcgaa agcgcccagc
     3421 agcagcagca cgagagcgat cagcggagtg agctgggact gcaggaaact ccgggcgatg
     3481 cggccggaga tgccgaggga tgaaccggcg tctttcatgg ccgactcagc ggactgcgag
     3541 cggattcagt agcacacgct cgccatcgcc caggccagca agcacttcga tgccgccgcc
     3601 ggccacggag ctgccgacac ggacttgccg caactgcggc cgcccgtcgg tggtgacgac
     3661 gttgaccgcc gtgagctcgc cccgccggat cagcactgcc tcgggcacca ccagcttgcg
     3721 agtccggccg atcgggatga ggatcttcgc ggcggcaccc ggcgtgacgc ccggtgtatc
     3781 gcccacgggc agatccgcac gaacctgcgt ggacaagagc ctggcgtccg ttgctggaag
     3841 gatcgtcact cgagtggcaa tcagtgtgcg tccaagttgc ggcaactcga ccgtcgcccg
     3901 ggtcgcaccc gccgctttcg gcagcacgaa ctgcggcagg ttgccgacag cgcgcagggc
     3961 tgagggatcg tgcacggcca cgactccgcg ccctggggcc gcgacctcgc ccggctccat
     4021 cagccggcgc gtgatcacgc cgtcgatcgg tgacttgagt tcggcgtggc tgcgcgcggt
     4081 attggcctgc gtgttgcctg cccgggcgga gtcggcggct gcctgcgcgg ccttgaagtt
     4141 cgattccgcc tggtcgagcg cagctcgact gacgaagttc ttctcgagca gattctgcgt
     4201 ccgttcgagg ttcaggcggg cctgtgtgag ctgggcgtcg gcctgggcga cgctcgcccg
     4261 acctgcagcc acctgtgcat cggtgtcgcg cgtgtcgatg cgcgctagca cctgaccttt
     4321 cttcacccgg tcgccggcgt caacgaagat ctgcgtgacg gtccctgaca gctgggccgc
     4381 gacggtcgcc tgccgcacgg cttcgatcac tgcgtcggct gcgtagacct cttccacgtc
     4441 cttcatgcgg acctcgacag tcgctgcagg ggtcgccgcc gttgtcaccg aaggcgtgcc
     4501 gttgtcggcg aggaccgcgc tactgctcaa cagcagcaac agggccagcg caacccggcc
     4561 ggccaggcgc ggccggttgg gaacggtggc gtgacgatgg ctgcacatgg cgaggggtgg
     4621 aaggagactt acgtggtgcg gcaagtgttg atgccgaaga gggagtaggc ggggcagctg
     4681 cgcatcaggc cggtcagcag cggaacgacg ccgacatagc cccagacgcc gatcgtgccg
     4741 gtggcggcaa gggcgatcag ggcgattcca ccgacgatgc gcaggatgcg gtccaggttt
     4801 ccgacgtttg cggtcatgat ggtcctcaag taacaagatc tgtgattcgg tggccccgct
     4861 tccggggaga cgggcgccgg ggtcattccg gctgcgcagt atagcgtgat atgccatacc
     4921 ccctagggta tattgctgtg atttgaccca tctcaagtgt cgggccgcca atcgcggcaa
     4981 actgaccgga gtaagggggc tccagagcgg atggcgccct gaggttcagt gttggttgcc
     5041 acctctgctt gtcgcggcgg cgtgatgcat tgcggcttga cagttcgata tgcgtcgtgg
     5101 aacatatgcg ttgttgcgca tataaacata ggcttcatgg aacccgagct ggagcggctg
     5161 ttcgaggtgg tatcggggta tttcggcctg ctggccgaac cgacccggct gaagatcctg
     5221 aacacgatct gtgacggcga gcgcagcgtg tctgacatcg tcgatcgggt gggttcgagc
     5281 cagacgaacg tgtcgcgcca tttgaacctg atgtacgggc gcggcgtgct gaagcgccgc
     5341 cgcgatggag cgatggtgta ctactcgatc agcgatccta atgtcgtcac gttgtgtcgc
     5401 accgcctgcg tgcatatcgc gggtcagcag gacgatcact cggtcacgag ccggacgctg
     5461 aagcgattca tgccggcgaa gtagcggctg ccggtgcgtc gaaactagaa gaagagcatg
     5521 ggagtgcaga tgagtaaagg agaacgagcc gtggcgcccg ggcgcatcgt cgcggcgccc
     5581 gaaagccacc tcacggccga gatgctgcgc ggcgaggcgc gatcgcgacg cagcttcctg
     5641 caaggtggcg cagctttggc aggcgccgtc gcggcggtgg cagtcgggcg ggcaggtgcg
     5701 caggaaaaag cggccttcga gccgcccaat gttccatcgt ggacacgatc tctcggtgca
     5761 cccgtcgtcg ccaagccgta tggcacgccg tcggagttcg aggccaacgt cat
//
LOCUS       DATIRT010000430         7807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_72062, whole genome shotgun sequence.
ACCESSION   DATIRT010000430 DATIRT010000000
VERSION     DATIRT010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7807
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72062"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VLN25_09680"
     CDS             complement(<1..428)
                     /locus_tag="VLN25_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HSQ10867.1"
                     /translation="MSPAELMDNVAALDSRADLDTARFPADARLALRLLDRMRQGRLH
                     LTFPDGQHGYYGDLRSPERADLRLANWNLVSAALRSGDIGFAESYIAGDWDSPDLIPV
                     LEFFIANRDAADEFIYGSFLGRLAYSIRHLLNRNTRRQSL"
     gene            complement(415..1206)
                     /locus_tag="VLN25_09685"
     CDS             complement(415..1206)
                     /locus_tag="VLN25_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HSQ10868.1"
                     /translation="MPSAPSAPMIGFGQIRHVRLRPARNAFTYPGYFLRLPLRTLARH
                     RLASRVLRINGRGIFAVNDRDHGDGRPLLAWIDDLLRSRGVRDADGEIWLHTFPRVLG
                     YVFNPVSFWFAHRADGALRAVVCEVNNTFGERHCYLLAHDDGRPLQYGEAVESRKVFH
                     VSPFCRVEGSYRFRFMLSSPAPGAAPRWVGRIDYDDDDGPLLLTSIAGTLAPLSDRAL
                     LRAFVAYPVFTFGVVARIHWQALRLWLKRGPFISTPEPPALHVTR"
     gene            1372..2001
                     /locus_tag="VLN25_09690"
                     /pseudo
     CDS             1372..2001
                     /locus_tag="VLN25_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(2032..3321)
                     /locus_tag="VLN25_09695"
     CDS             complement(2032..3321)
                     /locus_tag="VLN25_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSQ10869.1"
                     /translation="MRVAVIGSGISGLGCAHALRHDAHVTLYEAEPRLGGHTNTVDIT
                     LDGINYPVDTGFLVFNERTYPNLIRLLAELGVPTAKSEMSFAVSVQHGRRRIEWSGTD
                     LNSVFAQRRNLLSPRFLAMLRDILRFNAQATRIAQGQEAIEARVSLGAFLDEHGYGEP
                     FRQWYLLPMAAAIWSCPMATMVAYPVTTFVRFCHNHGLLQVNERPQWYTVKGGARQYV
                     HRIASGLHEVRTNEPVLEVRRDGGSRRIIVRSRSGAASYDHVVLACHSDQARRLLVDA
                     DADERGVIGKVSYQPNRAWLHTDERLMPSSRRVWSSWNYMSAGGSDPAVSVTFLLNKL
                     QPLPFSTPVFVSLNPVVAPRAEFVLGEFDYTHPVFDEAAIEAQQRLGGIQGRRGIWFA
                     GAWTGYGFHEDGLKSGLAVAAALRRVLATPSPLQRVA"
     gene            complement(3477..4280)
                     /gene="folE2"
                     /locus_tag="VLN25_09700"
     CDS             complement(3477..4280)
                     /gene="folE2"
                     /locus_tag="VLN25_09700"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006765498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HSQ10870.1"
                     /translation="MNAPDTMYLPDIQSAADARNLAIQRVGVKSLVHPMMVATARGPQ
                     PTVASVDMYVALPAEQKGTHMSRFIDVLQSHRDALSRASVHALLERMLQRLDAHDGFI
                     ELRLPYFVLKTAPVSGVESLMDYQLTLAIERRAATTRYLQKVVVPVTSLCPCSKKVAD
                     YGAHNQRAHLTLTLTLADEMRVEDQIRLAEDAASCEVWGLLKRTDEKYVTERAYDNPK
                     FVEDVVRDVATALDADERVRAFVVEAENFESIHNHSAYALIERIKDSAG"
     gene            complement(4340..4903)
                     /locus_tag="VLN25_09705"
     CDS             complement(4340..4903)
                     /locus_tag="VLN25_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HSQ10871.1"
                     /translation="MNPFAQTLAVAAAAVLLSSVTPASAQTCPALLNHSFPKLQDDSP
                     QSLCQYGGNVILVVNTASFCGFTPQYEGLEKLHAQYAKRGLTILGFPSGDFGNQEKDS
                     NKEIADFCYNTYGVKFPMFAKSSVKGTTANPLFAALTRDTGQAPKWNFHKYLIGRDGK
                     VVASFPSQVEPLDKRLTSQIETLLGAK"
     gene            5067..6002
                     /locus_tag="VLN25_09710"
     CDS             5067..6002
                     /locus_tag="VLN25_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HSQ10872.1"
                     /translation="MKDRTVHGTLTAAIAAVERDTGLSKDTLRVWERRYGFPAPLRDA
                     SGERTYPMDQVDRLRLLRRLIDQGYRPGKIIGLPMAELERLADGMGAEPHTLATLDPA
                     DDLRQCVDCVRDHRLDDLRRLLSQALLRLGLARFITDAVAPLNTMIGEEWTRGRLQIF
                     EEHLYTEAMQSILRGAIATIPHSLAGPRVLLTTFPQEPHGLGVLMAEAMLALDGAYCV
                     PLGTQTPLPEIVSAARAQQIDVVALSFTPTVNPNHILDGLAELRLRLPAEIEIWAGGA
                     APVLRRRPPSGIRILTVLADIEPAVADWRTRHGPG"
     gene            6079..6180
                     /locus_tag="VLN25_09715"
     CDS             6079..6180
                     /locus_tag="VLN25_09715"
                     /inference="COORDINATES: protein motif:HMM:NF021526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2256 domain-containing protein"
                     /protein_id="HSQ10873.1"
                     /translation="MTWRRVWARHWADVRYCSDRCRRVRRPAPGNPT"
     gene            6177..7730
                     /locus_tag="VLN25_09720"
     CDS             6177..7730
                     /locus_tag="VLN25_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HSQ10874.1"
                     /translation="MTPQATHLVLVLGDQLAIDSPVFDGFDAASDAVVMIEAAGEAQH
                     VWSHKARIVLFVSAMRHFAAELQARGWPIVYVTLDEAEYPEPELAQRLARVIAGTGAR
                     ALRLVEPGEWRVDQAIRAVADSAQVALTVLEDTHFICARGDFARWARGKVSLRLENFY
                     RHLRARERVLLDGQGEPVVGRWNFDADNRAGFPRSGPGVVPEAAAFAPDAQTREVMAL
                     VERMFPGHPGSLDEFAWPVTRAQARTALKRFVDERLPAFGRYQDAMWTGMPTGWHALL
                     SSSLNLKLLAPREVIAAAEQAWREGHAPIESVEGFVRQVLGWREFVRGVYWLDMPALA
                     DANGYGHRRALPRWYWSGDTHMACMREVVGQTLRYGYAHHIQRLMVTGQFALLAEVEP
                     RQVCDWYLAMYVDAVEWVELPNTAAMALHAAGGRMTSKPYVASGAYVRRQSNYCGECR
                     YRPELRTGDRACPMTTLYWNFLDRHEPELRRNPRTRLMAASIARLSAQERGAIRDQAM
                     QMLDNLDSL"
BASE COUNT         1330 a         2487 c         2685 g         1305 t
ORIGIN      
        1 ctcagcgact gcctccgggt gttccggttc agaaggtggc ggatgctata ggcgagtcgg
       61 ccgaggaacg agccgtagat gaactcgtcc gctgcgtcac ggttggcgat gaagaactca
      121 agtacaggta tcaggtcggg cgaatcccag tcgccggcga tgtaagactc ggcgaagccg
      181 atgtcaccgc ttctcagcgc cgcactgacg agattccagt tggccaggcg aaggtcggcg
      241 cgctcggggg agcgcagatc gccgtagtag ccgtgctggc cgtcaggaaa ggtcaggtgg
      301 agccggccct gacgcatgcg gtcgagcagg cgcagcgcaa ggcgggcgtc ggcaggaaag
      361 cgcgcggtgt ccaggtccgc gcgcgagtcg agggcagcga cgttgtccat gagttcagcg
      421 ggtgacatgc agcgccggag gctccggcgt ggaaatgaag ggaccgcgct tcagccagag
      481 ccggagcgcc tgccagtgaa tgcgtgcaac cacgccaaag gtgaagaccg gatacgcgac
      541 gaatgcgcgc agcagcgccc ggtccgacag cggggccagc gtcccggcga tcgacgtcag
      601 cagcagtggt ccgtcgtcgt cgtcatagtc gatccgcccg acccagcgcg gggccgcgcc
      661 cggggcaggg gagctgagca tgaaccggaa gcggtagctc ccttcgacac ggcaaaacgg
      721 cgagacgtga aacaccttgc gcgactccac cgcttcgccg tactgcagcg gccggccgtc
      781 gtcgtgcgcc agcaggtagc agtgacgctc gccgaaggtg ttgttcacct cacagaccac
      841 ggcacgcagc gcgccgtcgg cacgatgcgc gaaccagaag ctcaccgggt tgaacacgta
      901 acccagcacg cgcggaaacg tgtgcagcca gatctctccg tcggcatccc tgacgccacg
      961 gcttcgcagg aggtcgtcga tccaggccag caggggccga ccgtcaccgt ggtcgcggtc
     1021 gttcaccgcg aagatgccgc ggccattgat ccgaagaacg cggcttgcca gacggtgccg
     1081 ggcgagcgta cgcagcggca gccgcaggaa gtagccggga taggtgaacg cgttgcgcgc
     1141 cggacgcagg cgaacatgac ggatctggcc gaagccgatc atcggggcgg aaggtgcgga
     1201 cggcatgccg gatcgcggtc gtcaggaggt ataggaactg gaagtgtcgg acagcaaagt
     1261 cgcggtagtt tacacaatga actgatcagt tcaagtggaa acggagtagt atgttccctg
     1321 aactgaaagt gactgacagg cggctgcccg ccggggccgc gaggagagcc catggaaacg
     1381 tccgtagccg caccgcgtcg actgtcgttg aaagaacagc aacttcgatt gcgcgaggac
     1441 gtcatcctgc aggccgtgaa cggcctgctg gcggaaaaag gctttgacct gatgacggtc
     1501 gacgaagtcg cggccgtggt cggcatcgcg aaggccagcc tgtacaagca tttcgaatcg
     1561 aaggaggcgc tcgcggctgc ggccatggtc cggctgctcg accgcacgct tgatgtgatc
     1621 gccgcgcagc cggacgcaat ggcgcccgtc gacaagctga aagccgtggt gaactgggcg
     1681 gtggcggagc atttgcgcgg ctagatgccg tcgttgccct ccacgcgctc gactctgcgt
     1741 gacgccctcg tgcgcaacaa ggcttacatc gatcggctga tcgaggtgag cgatcgcctc
     1801 ggagcctgga tcgagcttgc gcagggacgc ggcgagatcg ccgccgcttt gccgccggag
     1861 gttgtgcttt acacgcttta tgcgcgtgcg tgcgacccgg tgtcggacta cctcaagctg
     1921 tccggaaact ttcgcgatgc tgaaatcgtc gcgttggtca cgacgacgtg cttctccggg
     1981 ctcgccgccc gcacgtcctg attgcattgc cggactgacg ccggggtagc gtcaggcaac
     2041 gcgctgcagc ggtgatggtg tcgccagcac gcgccgaagc gctgcggcca ccgccaggcc
     2101 ggacttcagg ccatcttcgt gaaagccgta gcccgtccag gcgcctgcga accaaatgcc
     2161 gcgtcgcccc tggattccgc ccagtcgctg ctgtgcctcg atcgcagctt catcgaatac
     2221 cggatgggtg taatcgaact cgccgaggac gaactccgcg cgtggcgcga ccaccgggtt
     2281 cagcgagaca aatacgggtg tcgagaacgg caagggctgg agcttgttca gcagaaaggt
     2341 caccgacacg gcgggatcgc tcccgcccgc gctcatgtag ttccacgatg accatacgcg
     2401 ccgtgatgac ggcatcagcc gctcatcggt gtgcagccac gcccggttcg gctggtagct
     2461 gactttgccg atgacgccgc gttcgtccgc gtcggcatcg accagcaggc ggcgcgcctg
     2521 atcgctatgg cacgccagca cgacgtggtc gtacgacgcc gccccggagc ggctgcgcac
     2581 gatgatccgg cggctcccgc catcccgccg cacttccagg accggctcat tggtgcgcac
     2641 ttcatgcagc ccgctggcga tgcggtgcac gtactgccgc gcgccgccct tcaccgtgta
     2701 ccactgcggc cgctcgttga cctgcagcag accgtggttg tgacagaagc gaacgaaggt
     2761 cgtcaccgga tacgccacca tggtggccat cgggcacgac cagatcgccg cggccattgg
     2821 caacaggtac cactgccgga acggctcgcc gtagccgtgc tcgtcgagga aggcgcccag
     2881 cgagacacgg gcttcaatgg cctcctggcc ttgcgcgatc cgtgtcgcct gcgcgttgaa
     2941 gcgcaggatg tcgcgcaaca ttgcgaggaa gcgcggggac agcaggttac gacgctgcgc
     3001 gaagacgctg ttcaggtccg tgccgctcca ctcgatccgt cggcggccgt gctgcacgct
     3061 gacggcgaac gacatctcac tcttcgcggt cggcacgccg agttccgcga gcagccggat
     3121 caggttcgga taggtccgct cgttgaacac caggaagccg gtgtccaccg ggtagttgat
     3181 gccatcgagc gtgatgtcga cagtgttggt gtggccgccg agccgcggct cggcttcgta
     3241 cagcgtcacg tgcgcgtcgt gtctcagggc gtgtgcgcaa cccaggccgg agatgccgga
     3301 accgatgact gcaactctca ttcgtgcggg tcctaaaagg ggaaaccatc ggacgcgcac
     3361 gagcgcggtc cggccgactc atcaccgagc ctccctcacg aatgacatgc ggccacttct
     3421 gcggcaggag ccgcggggcc gtccttggcg cggggcttct cctccccgcc gggcatttag
     3481 cccgcgctgt ccttgatgcg ttcgatcaag gcgtaggccg aatggttatg tatggattcg
     3541 aagttctcgg cttcgacgac aaaggcgcgc acgcgctcgt cggcgtcgag agccgttgcg
     3601 acgtcgcgca ctacatcctc gacgaacttg ggattgtcgt aggcgcgttc ggtcacgtac
     3661 ttctcgtcgg ttcgcttcag caggccccac acttcacacg aggctgcgtc ctctgccagg
     3721 cggatctggt cctcgacgcg catctcgtcg gccagcgtca gcgtcagcgt caggtgcgca
     3781 cgctggttgt gggcaccgta gtccgcgacc ttcttgctgc aggggcacag gctggtcacc
     3841 gggacgacga ctttctgcag gtagcgggtg gtcgccgcgc gccgttcgat ggccagcgtc
     3901 agctggtagt ccatcaggct ttcgacaccc gacaccggcg cggtcttgag gacgaagtac
     3961 ggcaggcgca gttcgatgaa gccgtcgtgc gcgtcgaggc gctgcagcat gcgttcgagc
     4021 agcgcgtgca ccgaagcgcg cgaaagggca tcccggtgcg actgcagcac gtcgatgaag
     4081 cgcgacatgt gcgtgccctt ctgctcggcc ggcagggcga cgtacatgtc gaccgaggca
     4141 acggtcggct gcgggccgcg tgcggtcgcc accatcatcg ggtgcacgag cgactttacg
     4201 ccgacgcgct ggatggccag gttgcgcgcg tccgccgcgg actggatgtc cgggaggtac
     4261 atggtgtcgg gagcgttcat ggcaaggccg ctattgtcgc cgaagtgccg ctgcgcccac
     4321 gcgcgcgccg cctcggcatc tacttcgcgc caagcagggt ttcaatctgt gacgtcaggc
     4381 gcttgtcgag cggttccacc tggctcggga acgaagccac gaccttgccg tcccggccga
     4441 tcaggtactt gtggaagttc cacttcggtg cctgtccggt gtcgcgggtc agtgccgcaa
     4501 acagtgggtt ggcggtggtg cccttgaccg aactcttcgc aaacatcgga aacttcacgc
     4561 cgtaagtgtt gtagcagaag tcggctatct ccttgttgct gtccttttcc tggttaccga
     4621 agtcgccaga cgggaacccg aggatcgtga ggccgcgctt ggcgtactgc gcgtgcagtt
     4681 tctccagtcc ttcgtactgg ggcgtgaagc cgcagaagct tgcggtgttg accaccagga
     4741 tgacgttgcc gccgtactga cacaggcttt gcggactgtc gtcctgcagt ttcgggaacg
     4801 aatggttcag cagcgccggg caggtctgcg cggaggcagg cgtaaccgag gacagcagta
     4861 ccgcggcggc ggcgacggcc agggtctgag cgaaaggatt cacggaaagg cggaccgatc
     4921 gatcggtgcg gcagtgacag cgggacgcga atgatcccaa gcccaggggc cttgtgtcaa
     4981 tgttttgtct aggacaaaat acgggacaca tatcgccact tgacgctcgg cttgccccta
     5041 gcccgggtcc cgtcccgcca tcacgaatga aagatcgcac tgtgcacggc acgctgaccg
     5101 cggctatcgc cgcggtggag cgcgacaccg gcctgtcgaa ggacacgctg cgcgtctggg
     5161 agcgacgcta cgggtttccg gcgccactgc gggacgcgtc cggcgaacgc acctacccga
     5221 tggatcaggt tgaccgactg cgcctgctca ggcggctcat cgaccagggg taccggcccg
     5281 gcaagatcat cgggctgccg atggcggaac tggagcgcct cgccgacggg atgggtgccg
     5341 aaccgcatac gctcgcaacg cttgatccgg ccgacgacct caggcagtgc gtggactgcg
     5401 tgcgggatca tcggctcgac gacctgcggc gcctgttgtc ccaggcgttg ctgcgcctcg
     5461 gcctggcgcg cttcatcacc gatgcggtcg ctccgctcaa cacgatgatc ggcgaggagt
     5521 ggacgcgcgg gcgcctacag atcttcgagg agcacctgta caccgaggca atgcagtcga
     5581 tcctgcgcgg agcgattgcc acgattccgc actccctggc cggaccccgc gtgctcttga
     5641 cgacctttcc tcaggagccc cacggtctcg gcgtgctgat ggccgaagcc atgctcgcgc
     5701 tcgatggcgc ctattgcgtg ccgctcggca cgcagacgcc gctgccagag atcgtttcgg
     5761 cggcccgcgc gcagcagatc gatgtggtgg cgctgtcgtt cacgccgacc gtcaatccca
     5821 atcacatcct cgacggactg gccgaactgc ggttgcgact gcccgctgag attgagatct
     5881 gggccggggg cgccgcgccc gtgctgcgcc ggcgcccgcc gagtggcatt cgcatcctga
     5941 cagtgctcgc cgacatcgag ccggcggtcg ctgactggcg cacgcggcat ggcccgggct
     6001 gaccgctccg gcgctcccgg gcacaaaggc aacaagtcgg gcctgcttgc caagtcctgt
     6061 gcggcgtgcg gccggccaat gacctggcga cgcgtgtggg cgcggcactg ggcggacgtg
     6121 cgctactgct ccgaccgctg tcgtcgggtg cgtcgaccag cgccgggcaa cccgacgtga
     6181 ccccgcaggc cacacacctg gtcctggtcc tgggcgacca gttggcgatc gactcgcccg
     6241 tcttcgacgg attcgatgcc gcctccgacg cggtcgtgat gatcgaggcc gccggtgaag
     6301 cgcagcacgt ctggagccac aaggcgcgca tcgtgctctt tgtctcggcg atgcgtcact
     6361 tcgcggccga actgcaggcc cgcgggtggc cgattgtcta cgtgacgctc gacgaagcag
     6421 agtatccgga gccggagctg gcgcagcgac tggcgcgcgt gatcgcaggc accggtgcgc
     6481 gcgcactgcg gctggtcgag ccgggcgaat ggcgagtcga ccaggcgatc cgggcggtag
     6541 cggacagcgc gcaagtcgcg ctgacggtcc tcgaggacac tcacttcatc tgcgcgcgcg
     6601 gcgacttcgc gcgctgggcg cggggcaagg tcagtcttcg gctggagaac ttctaccggc
     6661 acctgcgcgc gcgcgagcgc gtgctgcttg atgggcaggg cgaacccgtc gtcggccggt
     6721 ggaactttga cgcggacaac cgggccggat ttcccaggtc cggccccggc gtcgttccgg
     6781 aggctgcggc gtttgcgccg gacgcgcaga cacgcgaggt catggcgctg gtcgagcgca
     6841 tgtttccggg acatcctggg agcctggacg agtttgcatg gccggtgacg cgggcgcagg
     6901 cgcgcactgc gctgaaacga tttgtcgacg aacgccttcc tgcgttcggc cgctatcagg
     6961 acgcgatgtg gaccggcatg ccgaccggct ggcatgcgct gctgtcgtcg agcctgaacc
     7021 tgaaactgct ggccccgcgc gaagtgatcg ccgcggcgga gcaagcctgg cgcgagggtc
     7081 acgcaccgat cgagagcgtc gaaggcttcg tgcggcaggt cctcggctgg agagaattcg
     7141 tgcgtggcgt gtactggctc gacatgccgg cgctggccga cgccaacggc tacggtcatc
     7201 gtcgagcact gccgcgctgg tactggagcg gcgacacgca catggcgtgc atgcgcgaag
     7261 tggtcggcca gacgctgcgt tacggctatg cccaccacat ccagcggctg atggtgaccg
     7321 gacagttcgc gctgctggcg gaggtcgagc cgcgccaggt gtgcgactgg tacctggcga
     7381 tgtacgtgga cgcggtcgag tgggtcgaac tgccgaatac ggccgcaatg gcgctgcacg
     7441 ctgccggcgg ccgcatgacg agcaaaccgt atgtcgccag tggagcctac gtccggcggc
     7501 agagcaacta ctgcggcgaa tgccgctatc gcccggaact gcgcacgggt gatcgcgcct
     7561 gtccgatgac gacgctgtac tggaacttcc tcgatcgcca cgagcccgag ctccggcgca
     7621 atccgcgtac ccgactgatg gcagcctcga tcgcgcggct gtcagcgcag gagcgcggtg
     7681 ccatccgtga ccaggcgatg cagatgctgg acaatctcga ctcgttgtga cctccgcgag
     7741 cgagtgaaga gcaatgaaac tagccaaccg ggtggccgtg atcacggggg ccgcccaggg
     7801 catcggc
//
LOCUS       DATIRT010000431         1896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_761634, whole genome shotgun sequence.
ACCESSION   DATIRT010000431 DATIRT010000000
VERSION     DATIRT010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761634"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VLN25_09725"
     CDS             <1..783
                     /locus_tag="VLN25_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HSQ10875.1"
                     /translation="ARDGSGMQRDDWFSSSRLHAELASPEAIGDYAARRALARLGARK
                     LGTRQCPVLFEAPLACGLLGSFVQAASGGALYRKSTFLVDSLGKQIFAPHITIEEDPY
                     LPRGSGSSPFDEEGVRGLCRQVVSKGRLDGYFLSTYSARKLGMKSTGNAGGSYNLTLR
                     SSNTDDEDDLEVLLRRLGTGLLVTELIGQGVNYVTGDYSRGASGYWVEGGRIRHPVEE
                     ITIAGNLPQMFTGIAAVGSDTVVRGSKITGSILIDGMTVAGS"
     gene            932..>1896
                     /gene="dctP"
                     /locus_tag="VLN25_09730"
     CDS             932..>1896
                     /gene="dctP"
                     /locus_tag="VLN25_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HSQ10876.1"
                     /translation="MQRRKFLARATAGAAAAAAVTAPAIVRAQATVRWRLASSFPKSL
                     DTIYGAADVFSKQVSAATGGKFTVSVHAAGELMPAFGVLDGVQNGTVEAAHTAPYYFF
                     GKDPTFAMDCAIPFGLNSRQMTAWMYEGNGMKLFREFYRGFNIVNFPMGNTGAQMGGW
                     YRKEIKSLADMKGLKMRIGGFGGKVLERIGSVPQNIPGGEIYTALEKGTIDAAEWVGP
                     YDDLKLGFFKVAPNYYYPGWWEGGPQLTLFVNTKAWDALTPEYKGIVEACASHAHVNM
                     QAAYDGKNPGALKQLVAGGTKLHRFPKDVMDAAFKAAWEVYGELSA"
BASE COUNT          343 a          578 c          644 g          331 t
ORIGIN      
        1 gcccgggacg gcagtggcat gcagcgcgac gactggtttt cctcctcgcg cctgcacgcc
       61 gaactcgcct cgccggaagc catcggtgac tatgcggcgc ggcgcgcgct ggcccgactc
      121 ggcgcgcgca agctgggcac gcgtcagtgc ccggtgctgt tcgaggcgcc gctggcgtgc
      181 gggctgctcg gcagtttcgt gcaggccgcc agtggcggcg cgctgtaccg gaagtcgact
      241 ttcctggtcg actcgcttgg caagcagatc ttcgcgccgc atatcaccat cgaagaggac
      301 ccgtacctgc cgcgcggatc cgggtcgagc ccgttcgacg aggaaggggt gcgcgggctg
      361 tgccggcagg tcgtcagcaa gggtcgcctc gacggttact ttctgtcgac ctattccgcg
      421 cgcaagctgg gcatgaagtc gaccggcaat gccgggggct cctacaacct gacgttgcga
      481 tccagcaaca cggacgacga ggacgacctc gaggtcttgc tccgtcgcct cggtaccgga
      541 ttgctggtaa cggaactgat cgggcagggc gtcaactacg ttactggcga ctattcacgc
      601 ggcgcgagcg gctactgggt cgagggtggc cggatccggc atccggtcga ggaaataacg
      661 atcgccggca acctgccgca gatgtttacc ggcatcgcgg cagtggggtc cgataccgtc
      721 gtccgaggca gcaagattac gggctcgatc ctgatcgacg ggatgacggt cgccgggtcc
      781 tgaccgacct caacaggagg aaaagtcggc gttgccacgc cgtccgcagt ggatacaatg
      841 ccccggcgtt ggatcggtgg ccgcggcctt gcctcgcccc tcttcgctga gacatccgct
      901 gtgactctac aagtcaatca aggagacttt catgcaacgt cgtaaattcc tggcccgcgc
      961 gacggcgggc gccgcagcgg ccgcggcagt taccgcgcca gcgatcgttc gggcccaggc
     1021 caccgtgcgc tggcgactgg cgtcgagttt ccccaagtcg ctcgacacga tctacggggc
     1081 ggccgatgtg ttttccaagc aggtgtcggc agcgaccggc ggcaaattca cggtcagcgt
     1141 ccacgcggcc ggtgaactga tgccggcttt cggggtgctt gatggcgtcc agaacggcac
     1201 cgtggaagcc gcgcataccg cgccgtatta cttcttcggc aaggacccga ctttcgcgat
     1261 ggactgcgcg attccgttcg gactgaattc ccgacagatg acggcgtgga tgtacgaagg
     1321 caacggcatg aagctgttcc gcgagttcta tcgcggtttc aacatcgtca atttcccgat
     1381 gggcaatacg ggcgcgcaga tgggcggctg gtaccgcaag gagatcaagt cactggcgga
     1441 catgaagggc ctgaagatgc gcatcggcgg cttcggcggc aaggtgctgg aacgcatcgg
     1501 ctcggtgccg cagaacattc cgggcggcga gatctacacc gcgctggaaa agggcacgat
     1561 cgacgcggcc gaatgggtcg gtccctatga cgacctgaag ctcggcttct tcaaggtcgc
     1621 accgaactac tactacccag gctggtggga aggcggcccg cagctgacgc tgttcgtcaa
     1681 caccaaggct tgggacgcgc tgacgccgga gtacaagggc atcgtcgaag cgtgcgcgtc
     1741 gcatgcgcac gtgaacatgc aggctgccta cgatggcaag aatccgggcg ctttgaagca
     1801 actggtggcg ggcggaacga agctgcaccg attcccgaag gacgtgatgg acgcggcgtt
     1861 caaggccgcc tgggaagtct atggcgagct gagtgc
//
LOCUS       DATIRT010000432         6079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1253529, whole genome shotgun sequence.
ACCESSION   DATIRT010000432 DATIRT010000000
VERSION     DATIRT010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6079
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253529"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VLN25_09735"
     CDS             <1..323
                     /locus_tag="VLN25_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSQ10877.1"
                     /translation="QAVRLVLSGGTYAPLRLLGGLGGPLPVGNGDSTLGRDLPVHGLT
                     QRQLDVLGLLARGLPNKLIARELGLSEGTVKVHLLAIFRALDVRNRTEAVVAAQKKLQ
                     ATGP"
     gene            complement(360..1049)
                     /locus_tag="VLN25_09740"
     CDS             complement(360..1049)
                     /locus_tag="VLN25_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10878.1"
                     /translation="MRKLFASFGVMCLASAASAGPAIVAHSACPQYAVDIEAFATCDG
                     DRVAMPEDIRLGDDLLLAEADIPASKRTFASLYVDAPRAYVLKRRYPEAVVLVDIRST
                     LELGMTGHAEGVDLNVPYYEFVQPLVWNEQTHGWTMAANPRFVAQLDQRLQQHGVSPD
                     TVVMLICRSGDVTARATDELTLRGYRRVVSVVYGYEGDLGPDGQRARNGWKNAGLPWI
                     ARANTALIEAR"
     gene            complement(1158..1394)
                     /locus_tag="VLN25_09745"
     CDS             complement(1158..1394)
                     /locus_tag="VLN25_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10879.1"
                     /translation="MRLARGLRAVAATVIIGGIAIAALDTTPTTELEISRDAIADPAG
                     SPAIGAPLSADPSAPASRELESPLERIERSMEHH"
     gene            1721..2608
                     /locus_tag="VLN25_09750"
     CDS             1721..2608
                     /locus_tag="VLN25_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10880.1"
                     /translation="MNEMTALHLLRDGASAAAELIALLFMAPFATATAAESTPPTTTP
                     AAVVATAEDAAPSAAWLAARELHVTGAALDVRLLGMLADVRVVQTVRNDAVQSVDLAA
                     RLPATNDGLGRLSIARAGRSIELPAEPGDDCSVDDDDPHAGHVRMALDEVLADLLHLP
                     PGQRATVDVGTTATLERLGAAYRFNLPATIAPLDAQALLLEQPAGPTLIVVAPARAEG
                     VATLTLRPASGPARIIPLGHTTAGSAIVVPLADADALVQLAHGAIELEFRTDDRVLWT
                     TLPSTVRSARDATLARAAD"
     gene            2621..4660
                     /locus_tag="VLN25_09755"
     CDS             2621..4660
                     /locus_tag="VLN25_09755"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HSQ10881.1"
                     /translation="MHRFASTRLSTPKLYLPVLQAVLAILVALPIGVRAETADRADRT
                     LSPYFVVNHAEPGVDALPLKATSVDVKVSGVIADVRVTQRYRNEGSQPIEARYVFPAS
                     TRAAVTALTMRVGDRVVKAQIREKQAARRDYEGAKQAGKSASLLEQQRPNVFQMSIAN
                     LMPGDTLEVELRYAESIVPTDGLYRFDFPTVVGPRYNGTPGSESHQSEPWIAQPTLRA
                     GVAPTPTFAMNVEITSPIPVLEVRSPSHRFNVDGTGSRSLRAALPAAHDHANRDVVLE
                     YRLAGREIASGVLVHEGKDENFFLAMIEPPARVPAGAIVPREYVFILDVSGSMHGFPI
                     ETAKTLLRGLVATLQPTDTLNIIPFAGGHSMLAPRSLPATRTNVDAAVRFINAQRGGG
                     STELLSALRGALAMPSDPDRARSFVVITDGYVSIEKEAMDLVRENLGQANVFAFGIGS
                     SVNRFLIEGLARAGRGEAFVVLNPKQAAVEADRFRAYIEAPLLTRIRVGFRGFDAYDI
                     DPPHVPDLFAQRPIVLSGKFRGAPSGSIEIEGMTATGPFKRSIDVASHRSEERDTQAL
                     ATLWARSRIATLGDYQKLVNDSAAASAITALGLRYSLLTDYTSFIAVDQVVRNPTGEQ
                     RSVDQPQPLPEGVSELAVGAVPSTPEPEFVSMAVATGGLMWWLRRRRRRESVHAN"
     gene            4650..5540
                     /locus_tag="VLN25_09760"
     CDS             4650..5540
                     /locus_tag="VLN25_09760"
                     /inference="COORDINATES: protein motif:HMM:NF021254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase/exosortase family protein"
                     /protein_id="HSQ10882.1"
                     /translation="MRTELNARIGRTVPSPLFGLLVGSPDTGHAAPLGHRRSHRIWSR
                     ATWMPVALLLALWPHWVYVTRRLWDGSDEPWGVLALVTVVALLIRDRAQLVLPTRSAL
                     VACGVLAVVAAFADRALPDLIAAAIAMLALGVFVVYALRRPAAAIIVLLLLTLPIIAS
                     LQFYLGYPLRLMVAHASAQLLSIAGWHAEASGASILHEGITVLVDAPCAGIGMLWLGS
                     YAAALLSYLNRASARRTLLNSVVAALLVLIANIVRNTLLFFPEAGLVAWPGAAHGAIG
                     LVAFAGAIVPLTLFAAWGKQ"
     gene            5537..>6079
                     /locus_tag="VLN25_09765"
     CDS             5537..>6079
                     /locus_tag="VLN25_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10883.1"
                     /translation="MNVRTFFVGALLVAGVLPLSVHYTLHPAGGRAVWPHWPHAVEGD
                     ADPRIDWPTHFRDQPLTQLPLSDLEERFARRFPGAIARFTDGRRMLILRRVQRPTRQL
                     HPAADCFKALGYTIDRPRPVVDAEDAKWSCFAAARDGARLRVCERIHDSAGNAWTDTS
                     AWFWAAQYGGGPWWATTVVEP"
BASE COUNT          932 a         2090 c         1979 g         1078 t
ORIGIN      
        1 tgcaggctgt gcgcctggtg ctgtccggcg ggacctatgc gccgttgcgg ctgctcggcg
       61 gcttgggcgg cccgctgccg gtgggcaacg gcgactcgac cctgggacgc gaccttcccg
      121 tccatggact cacgcagcga cagctcgatg tactcggcct gcttgcccgc gggttgccga
      181 acaagctgat cgcgcgcgaa ctggggctct ccgaaggtac ggtaaaggtc cacctgcttg
      241 ccatcttccg cgcactcgat gtgcgcaatc gcaccgaagc agtcgtggcc gcacaaaaaa
      301 aactccaggc gaccggcccc tgagggacga accgcccgga gtgccaggga gacagtgggt
      361 cagcgcgctt cgatcaacgc ggtattggct cgagcaatcc atggcaggcc cgcgttcttc
      421 catccgttgc gtgcacgctg gccatccgga ccgaggtcgc cttcgtagcc gtaaacgacg
      481 ctgaccacgc ggcggtagcc gcgcagcgtg agttcatcgg tcgcgcgcgc cgtgacatcg
      541 cccgagcggc agatcagcat cacgactgtg tcgggcgaca caccgtgctg ctgcagacgc
      601 tgatcgagtt gggcaacgaa gcgcggattg gccgccatcg tccagccgtg ggtctgctcg
      661 ttccagacca gcggctggac gaactcgtaa tacggaacgt tgaggtcgac cccctccgcg
      721 tggcccgtca tgccgagttc caacgtactt cggatgtcga ccagcacgac tgcctccgga
      781 tagcgccgct tgaggacgta ggcgcgcggc gcgtcgacgt agagcgaggc gaacgtacgc
      841 ttgctggccg ggatatcggc ttcggccagc agcaggtcgt ccccgagcct gatgtcctcc
      901 ggcatggcga cccggtcgcc gtcacaggtc gcaaaggctt cgatgtcgac cgcgtattgc
      961 gggcaggcgc tgtgcgcaac gatcgctggg ccggcacttg ccgctgaagc caggcacatc
     1021 actccaaacg acgcaaacag tttccgcata ccggactcct ctgggtgtga acgctagcga
     1081 cagtgcaccg ggaaagcact gcaccactcg gcctatgtcg aacggcttac gtggcgcggt
     1141 tccgcccctg cgtaggtcta gtggtgttcc atgcttcgtt cgatacgttc gagcggagac
     1201 tccaactccc gcgatgccgg cgccgacgga tcagcactga gcggcgcgcc gattgccgga
     1261 ctgccggccg ggtctgcgat cgcatcgcgc gagatctcga gctccgtggt cggggtcgta
     1321 tccagggccg cgatcgcgat gccgccgatg atcacagtcg cggccacggc gcgcagtccg
     1381 cgggccagtc tcatcgtgcg cctctcttcg tcggtccgta gggacctcct gctgaagtcg
     1441 gcttgcatag gcctccctgt ttgttgaaga aagtcatcgt gctgtgaagt gaatcgtccg
     1501 cccgagcggt gaatccggac attcaccggg cgtcgtaggt cggcagttca tccggcctat
     1561 gccgaacggc acgccgcctt cacgcagcgt tcacacgggc tgcgcgtgca gttcttgcgc
     1621 ggttcgcaag cccttcgcat cgtccgcgga tcgttcgtcc cgtacccgcc gcgtcttgtc
     1681 caggcgggaa atcgatccgg gacacgaacc ggaagaggag atgaacgaga tgactgccct
     1741 gcatctactc cgcgacggcg cctccgctgc ggccgagttg atcgcgctgt tgttcatggc
     1801 gccatttgcg actgccacgg ctgctgaatc cacaccgccg acgacgacac ccgcggccgt
     1861 ggtcgcgacg gccgaggatg ctgcaccctc ggctgcatgg ctcgccgctc gcgagctgca
     1921 tgtcaccggc gcggcgcttg atgttcgcct gctcgggatg ctggccgacg tgcgcgtcgt
     1981 ccagaccgtg cgcaacgacg ccgtgcagtc ggtcgacctg gcggctcggc ttcccgccac
     2041 caatgacggc ctcggacggc tgtcgatcgc acgcgccgga cgcagtatcg aactgccggc
     2101 cgaacccggc gacgactgca gcgttgacga cgacgatccg catgccggtc acgtccggat
     2161 ggcactggac gaggtgctgg ctgacctgct gcacctgccc cccggtcagc gggcgacggt
     2221 cgatgtcggc accaccgcca ccctcgaacg cctcggcgct gcctatcgtt tcaacctgcc
     2281 ggccacgatc gccccgctcg atgcccaggc gctgctgctc gaacagccgg ccggtcccac
     2341 cctgatcgtt gttgcgccgg cccgggccga gggagtcgcc acgctgaccc tacgtcccgc
     2401 gagcggtccg gcccgcatca ttccgctcgg acacacgacg gccggaagtg ccatcgtcgt
     2461 tccgctggcc gatgcggatg ctctcgtgca gctcgcccac ggggcgatcg aactcgagtt
     2521 ccgtaccgac gaccgcgtgc tgtggacgac cttgccttcg acagtgcgca gtgcgcgtga
     2581 cgcgacgctg gcgcgcgctg cggactaatg aggagaagcc atgcatcgtt tcgcctctac
     2641 ccgcctgtcg acgccgaagt tgtacctgcc cgtgctgcag gccgtcctgg ccatactcgt
     2701 cgcgctgccc atcggcgtgc gtgccgagac cgccgaccgc gccgatcgaa cgctgtctcc
     2761 atacttcgtc gtcaatcacg ccgagcctgg cgtcgatgcg ctgccgctca aagcaacaag
     2821 tgtcgacgtc aaggtcagcg gcgttatcgc cgatgtccgc gtcacccagc gctatcgcaa
     2881 tgagggctcg caaccgatcg aggcgcgcta cgtgttcccg gcttcgacgc gcgctgccgt
     2941 cacggcactg acgatgcgcg tcggcgatcg tgtggtgaaa gcgcaaatcc gggagaagca
     3001 ggcggcgcgc cgcgactacg agggcgcgaa gcaggccggc aagagcgcat cgctgctcga
     3061 gcagcaacgc ccgaacgtgt tccagatgtc cattgccaac ctgatgccgg gcgacaccct
     3121 cgaggtcgag cttcgctatg ccgagagcat cgtgcccacc gacggcctct atcgcttcga
     3181 ctttccaacc gtcgttgggc cgcgctacaa cggtacgccg gggagcgaaa gtcatcagtc
     3241 cgagccgtgg atcgcgcagc cgaccttgcg cgccggcgtg gcaccgactc cgacatttgc
     3301 gatgaacgtc gagatcacga gcccgatccc ggtgctggag gtgcgctcac catcgcatcg
     3361 attcaacgtc gacggcacgg gtagccgcag tctccgcgct gcgctgcctg ccgcccacga
     3421 ccacgccaac cgtgacgtcg tgctcgagta ccgcctggcc gggcgcgaga ttgcctcggg
     3481 cgtgctggtg cacgagggca aggacgagaa cttcttcctc gcgatgatcg aaccgccggc
     3541 gcgcgtgccc gcgggagcga tcgtgccgcg cgagtatgtg ttcatcctcg acgtgtcggg
     3601 ttcgatgcac gggttcccga tcgagaccgc caagacgctg ctgcgcggcc tggtcgcgac
     3661 gctgcagccg accgatacgc tgaacatcat cccgtttgcc ggtggccatt cgatgctggc
     3721 accacggtcg ctgccggcga cgcggaccaa tgtcgacgcc gcggtgcgtt tcatcaatgc
     3781 gcagcgcggc ggcggatcga ccgaactgct gtcggcatta cgcggcgcac tggcgatgcc
     3841 ttccgacccg gaccgtgctc gcagcttcgt cgtcatcacc gatggctatg tgtcgatcga
     3901 gaaggaagcc atggacctgg tgcgcgaaaa cctgggccag gccaacgtgt ttgcgttcgg
     3961 catcggctcg tcggtcaacc gcttcctgat cgagggcctg gcgcgggccg ggcgcggcga
     4021 ggctttcgtc gtcctcaatc caaagcaggc agcggtcgag gccgaccgct tccgcgccta
     4081 catcgaagcg ccgctgctga cccgtatccg cgtcggcttc cgcggcttcg acgcctacga
     4141 catcgatccg ccgcacgtgc cggatctgtt tgcccaacgg cccatcgtgc tgtcgggcaa
     4201 gttccgcggg gcaccgtccg ggtcgatcga gatcgagggc atgactgcga cgggtccgtt
     4261 caaacgatcg atcgacgtag cctcacaccg gtccgaggag cgcgacacgc aggcactcgc
     4321 cacgctgtgg gcccgcagtc gcatcgccac gctcggcgac tatcagaagc tcgtcaacga
     4381 ctcggccgcc gcaagtgcga tcacggcgct cggcctgcgc tatagcctgc tgaccgacta
     4441 cacctcgttc atcgcggtcg accaggtcgt tcgcaatccc accggcgagc agcgcagcgt
     4501 cgatcagccg cagcccttgc cggaaggcgt cagcgagctt gcggtcggtg cggtgcccag
     4561 cacgccggaa cccgagttcg tctcgatggc cgtggcgacg ggtgggctga tgtggtggct
     4621 acggcgccgt cgtcggcggg agtcggtgca tgcgaactga actcaacgct cgcatcggtc
     4681 gtaccgtgcc gtcgccgcta ttcgggttac tcgtcggatc accggacacg gggcacgcag
     4741 cgccccttgg ccaccgtcgc tcgcatcgga tctggtcgcg tgccacgtgg atgcccgtcg
     4801 cgctgctgct ggccctttgg ccgcactggg tctacgtgac tcgtcggctg tgggacggat
     4861 ccgacgaacc ctggggcgtt ctcgcgttgg tgactgtcgt tgccttgctg attcgcgatc
     4921 gagcccagct cgtcctgccg acgcgctcgg ccctggtcgc ctgcggggtg ctggccgtcg
     4981 tcgccgcttt cgcagaccgt gccttgcccg acctcatcgc cgcggcgatc gcaatgctgg
     5041 cgcttggagt tttcgtcgtg tacgcactgc gccgtcccgc cgcggcgatc atcgttctgt
     5101 tgctgctgac gctgccgatc atcgcgtcgc tgcagttcta tctcggttac cccttgcgac
     5161 tgatggtggc ccatgccagc gcgcagctgc tatcgatcgc cggctggcat gccgaggcca
     5221 gcggggcgtc gatccttcat gaaggcatca cggtgctcgt cgacgcgccg tgcgccggca
     5281 tcggcatgct gtggctcggc agctacgcgg ccgcgttgct gtcgtacctg aaccgggcca
     5341 gcgctcgccg caccctgctc aacagtgtcg tcgcagcgct gctggtgctg atcgccaaca
     5401 tcgtgcgcaa cacgctgctg ttcttcccgg aagcgggtct tgtcgcctgg ccgggagccg
     5461 cgcacggagc gatcggcctc gtcgccttcg cgggagcgat cgtgccgctg acgctcttcg
     5521 cagcctgggg aaagcaatga acgtcaggac cttctttgtc ggcgccctgc tggtcgcggg
     5581 cgtgcttcca ctgtccgtgc actacacgct gcacccggcg ggaggacgtg cggtgtggcc
     5641 ccattggccc catgcggtgg aaggcgatgc ggacccacgc atcgactggc ccacccactt
     5701 ccgcgaccag ccgctgaccc aattgccgtt gtcggacctc gaggagcggt ttgcacggcg
     5761 ctttcctggt gcgatcgccc gcttcaccga cggacgccga atgctgatcc tgcgccgggt
     5821 gcagcgtccg acacgccagt tgcatcccgc ggccgactgc ttcaaggcac tgggctacac
     5881 aatcgaccgg ccacggccgg tcgtcgacgc ggaggatgcg aagtggagct gcttcgccgc
     5941 tgcgcgcgat ggcgcgcgac tgcgcgtctg cgaacgcatc catgacagtg caggtaacgc
     6001 ctggaccgat acctcggcct ggttctgggc cgcgcaatac ggcggcggac cctggtgggc
     6061 cacgacagtg gtcgagcct
//
LOCUS       DATIRT010000433         5789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1450134, whole genome shotgun sequence.
ACCESSION   DATIRT010000433 DATIRT010000000
VERSION     DATIRT010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5789
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450134"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="VLN25_09770"
     CDS             complement(<1..778)
                     /locus_tag="VLN25_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSQ10884.1"
                     /translation="MAAALTDSTTTLGTLEHVRAIAPYQAGKPIDELAREFGLDPASI
                     VKLASNENPLGAPESAKKAMAAAVEDLGRYPDSNGFELKAALGARYGVPMDWITLGNG
                     SNDILELAAAATLTPGRSCVYAQHSFAVYALATQARGGRAIVVPARELGHDLDAMFTA
                     IAPDTALVYVANPNNPTGTHLKAEAIDAFLARIPARVVVVLDEAYNEYLPPELRVDST
                     AWVRRYPNVVVSRTFSKAFGLAGLRVGYGIAQRELTDLMNR"
     gene            complement(796..1887)
                     /gene="pheA"
                     /locus_tag="VLN25_09775"
     CDS             complement(796..1887)
                     /gene="pheA"
                     /locus_tag="VLN25_09775"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HSQ10885.1"
                     /translation="MPESIDRRLKPLRDAIDGVDARLVELLNERARLASEVGRLKGEV
                     EAPVLRTEREAEVLRKVGTLSQGPLTAAPLANVFREIMSACRALERPLTVAFLGPAGT
                     FSETAMHRQFGTSVTGLPCATLDEVFRATEAGSADVGIVPLENSSEGAVSRTLDLLLA
                     TPLKLIAELSVPVDHNLLTHSGTMDGIECICAHSQALAQCAGWLAQHYPAIERRAVAS
                     NAEGARLAAESPTMAAIAGDRAAERYELVPVATHIQDDPNNRTRFAVLGQQMTRPSIP
                     PGRDKTSLILSVPNKAGAVFHMLEPLSRHGVSMTRFESRPARVGTWEYYFYVDVEGHQ
                     ADPKVATALAELQQACAFYKCLGSYPAEH"
     gene            complement(1880..2002)
                     /locus_tag="VLN25_09780"
                     /pseudo
     CDS             complement(1880..2002)
                     /locus_tag="VLN25_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761552.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
     gene            complement(2109..4745)
                     /gene="gyrA"
                     /locus_tag="VLN25_09785"
     CDS             complement(2109..4745)
                     /gene="gyrA"
                     /locus_tag="VLN25_09785"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HSQ10886.1"
                     /translation="MDQFAKETLPISLEEEMRRSYLDYAMSVIVGRVLPDVRDGLKPV
                     HRRVLFAMHESNNHWNRSYVKCARVVGEVMGKYHPHGDNAIYDTLVRMAQDFSLRYTL
                     IDGQGNFGSIDGDNAAAMRYTECRLDKIAGELLADIDKETVNFVPNYDGKEREPSVLP
                     SKIPNLLINGSAGIAVGMATNVPPHNLSEVIDGCLVLLRRSETTVDELIELIPAPDFP
                     TAGIIYGLEGVREGYRTGRGRVVMRAKTHFEEIDKGARSAIIVDELPYQVNKRVLLER
                     IAEMVNEKRLEGISDIRDESDKSGMRVVFELKRNEVPEVVLNNLYKQTQLQDTFGINM
                     VALVDGQPRLLNLKQMLDAFLSHRREVVTRRTVFELKEARKRGHVLEGLAVALANIDE
                     FIAIIKAAPTPPVAKTALMARAWDSSLVREMLTRSGADMAAYRPDGLEARFGLQSEGE
                     LRGLYRLSEEQAEQILQMRLQRLTGLEQDKIVNEYREVMETISDLLDILAKPARITDI
                     IADELLALKSEFGDTRRSEIVRSAQDLMTEDLIAREDMVVTLSHTGYIKSQPLADYRA
                     QKRGGRGKQAAAMREDDWIDQLFIANTHDTILCFSNRGRLYWLKVWEVPQGARTARGK
                     PIVNMFPLAEDEKITVVLPISEYDGQHFVFMATAMGTVKKTALTDFSNPRKAGIIAVD
                     LDPGDYLIGAAITDGQHDVMLFSDAGKAVRFDENDVRPMGRTARGVRGMQLDYGQQVI
                     AMLVAEDEEQSVLAATENGYGKRTPIVEYTRHGRGTKGMIAIATSERNGKVVAATLVR
                     GDDEVMLITTGGVLIRTRVSEIREMGRATQGVTLIALDDGTTLTGLQRVVERDSENGN
                     GHDPAASIETGEAPASGSTSED"
     gene            4960..>5789
                     /locus_tag="VLN25_09790"
     CDS             4960..>5789
                     /locus_tag="VLN25_09790"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSQ10887.1"
                     /translation="MESVDLVIEARWVVPVVPKGAVLEDHAVVVHEGRILALLPRDAA
                     QARYEPVERVVLATHVLTPGLVNLHTHAAMALFRGIGDDLPLMQWLQQRIWPLEKALV
                     SPEFVYDGTRIAALEMLRSGTTCCSDMYFYPEAAVRALRGVGMRAVAGIIAIEFPTAY
                     AADAEDYLRKGLATRDACRDDALVSFTLAPHAPYTVEDATLTKIAVLAEELDLPVHIH
                     LHETADEVAESVRRYGQRPLARLDRLGLVTERLIARCSTSTSPASSFATRQSRRTCSR
                     T"
BASE COUNT          942 a         1954 c         1819 g         1074 t
ORIGIN      
        1 cgcgattcat caggtcggtg agttcccgct gggcaatgcc gtagccgacc ctcagcccgg
       61 caagcccgaa ggccttcgaa aatgtgcgcg acacaacgac gttcggatac cgccgcaccc
      121 aggccgtgct gtcgacccgc agttcggggg gcaggtattc gttataggcc tcgtcgagca
      181 cgaccacgac gcgcgccgga atccgggcaa ggaacgcgtc gatcgcctcg gccttcaggt
      241 gcgtgccggt cgggttgttc ggattcgcga cgtagacgag cgccgtgtct ggcgcgatcg
      301 ccgtaaacat ggcgtcgagg tcgtgaccca gctcgcgcgc aggcaccacg atggcgcgtc
      361 caccgcgcgc ctgggtagcg agcgcataga cggcaaacga atgctgcgca taaacgcagc
      421 tgcgacccgg cgtcagcgtg gcggcggcgg ccagttcgag gatgtcgttg ctgccgttgc
      481 cgagagtgat ccagtccatc ggcacgccgt agcgcgcccc cagcgcagcc ttcagctcaa
      541 atccgttgga atccgggtag cgaccgaggt cctcgaccgc tgcggccatt gccttctttg
      601 ccgactctgg cgcgcccagt ggattctcgt tcgacgcgag cttgacgatc gacgccggat
      661 cgagcccgaa ctcgcgcgca agttcgtcta tcggcttgcc cgcctggtac ggcgcgatcg
      721 cacgcacatg ctcgagcgtg cccagtgtcg tcgtggaatc cgtcagagcc gccgccatgg
      781 tcgtccccaa acttgtcaat gttcggccgg ataggaaccg aggcacttgt agaacgcaca
      841 cgcctgctgc agctcggcca gcgccgtggc gaccttcgga tcggcctggt gtccctcgac
      901 gtccacgtaa aagtagtact cccacgtgcc gacgcgggcg ggacgcgatt cgaagcgcgt
      961 catcgacacc ccgtgccgcg ataagggctc gagcatatgg aacaccgccc cggccttgtt
     1021 gggcaccgac aggatcagcg atgtcttgtc acgcccgggc ggaattgatg gccgcgtcat
     1081 ctgctgcccc agcaccgcaa agcgcgtgcg attgttgggg tcgtcctgga tgtgcgttgc
     1141 gaccgggacc agttcgtagc gctccgccgc gcgatcgccg gcgatggcgg ccatcgtcgg
     1201 actttccgct gcgaggcgcg cgccttcggc attgctggcg acggcacgcc gctcgatggc
     1261 cggatagtgc tgcgccagcc agccggcaca ctgcgccagc gcctgcgagt gcgcgcagat
     1321 gcactcgatc ccgtccatcg tgccgctgtg tgtcagcaga ttgtggtcca ccggaaccga
     1381 cagctcggcg atcagcttca acggcgtcgc cagtaacagg tcgagtgtgc ggctgaccgc
     1441 gccttcggac gaattctcca gcggcacgat gccgacatcg gcgctgccag cctcggtcgc
     1501 gcggaagact tcatcgagcg tggcgcacgg caatccggtg acgctggtgc cgaactggcg
     1561 gtgcatcgcc gtctcggaaa atgttccggc aggccccagg aaagccactg tcaacggacg
     1621 ctcgagcgcg cggcacgccg acatgatttc ccggaacaca ttggccagcg gcgcggctgt
     1681 cagcgggcct tgcgacagcg tacccacttt gcgcagcact tccgcctcac gctcggtgcg
     1741 cagtaccggc gcttcgactt cacctttgag ccggcccact tcgctggcca gccgggcgcg
     1801 ttcgttgagg agttcgacaa ggcgcgcatc gactccgtcg atggcatcgc gcagtggctt
     1861 caggcgccgg tcgatcgact caggcatggc atcgctcgaa gtcccgcatg aaggcgacca
     1921 acacctgtac gcgggccagg ggcatggcgt tgtagatcga cgcccgcgtg ccgccgacga
     1981 gccgatgacc tttcagctgc actcgttcac gctccgcggc ttccgacagg agggtcgcgc
     2041 gtccgacgag gagaacggca agttcatacg cgacagtcgg gcggattggg gacaccccgc
     2101 aatccgactc agtcctcact cgtcgatcca ctcgccggcg cctcgccggt ttcgatgctc
     2161 gctgccgggt cgtgaccgtt gccgttctcg ctgtcgcgtt cgaccacgcg ctgcagcccc
     2221 gtcagcgtcg taccgtcgtc cagcgcgatc agagtcactc cctgcgtggc acgtcccatc
     2281 tcgcggatct cggacacacg ggtgcgaatc agcacgccgc cggtcgtgat cagcatgacc
     2341 tcatcgtcgc cgcgcaccag cgtggccgct acgaccttgc cgttgcgctc gctggtggca
     2401 atcgcgatca tgcccttggt tccgcggccg tggcgcgtgt actcgacgat gggcgtgcgc
     2461 ttgccatagc cgttctctgt cgcggccagc acgctttgct cctcgtcctc agccaccagc
     2521 atcgcgatga cctgctggcc gtaatcgagc tgcatgccgc gtacgccgcg cgcggtgcgg
     2581 cccattggcc gaacgtcgtt ctcgtcgaaa cggacggcct tgccggcatc ggagaagagc
     2641 atcacgtcgt gctggccatc ggtgatcgcg gcgccgatca ggtaatcacc cggatcgaga
     2701 tcgaccgcga tgatcccggc cttgcgcgga ttgctaaaat ccgtcagcgc cgttttcttg
     2761 accgtcccca ttgccgtggc catgaagacg aagtgctgcc cgtcgtattc agagatcggc
     2821 agcacgaccg tgatcttctc gtcttcagcc agcgggaaca tgttgacgat cggcttgccc
     2881 cgcgcagtgc gcgcgccctg cggcacttcc cataccttga gccagtagag acgcccgcgg
     2941 ttggagaagc acaggatcgt gtcgtgcgtg ttggcgatga agagctggtc gatccagtcg
     3001 tcctcgcgca tggccgccgc ctgcttgccg cgaccgccgc gcttctgcgc gcggtaatcc
     3061 gccaggggct ggctcttgat gtacccggtg tgcgagagcg tgaccaccat gtcctcgcgc
     3121 gcgatcaggt cctcggtcat cagatcctgc gcgctgcgca cgatctccga gcgacgcgtg
     3181 tcaccgaact cgctcttcag tgccagcagt tcgtcggcga tgatgtcggt gatgcgcgcg
     3241 ggcttggcca ggatgtcgag caggtcgctg atggtttcca tcacctcgcg gtactcgttg
     3301 acgatcttgt cctgctccag gccggtcaga cgctgcaggc gcatctgcag gatctgttcg
     3361 gcctgctctt cggacaggcg gtacaggccc cgcagttcac cttcgctctg caatccgaag
     3421 cgcgcctcga gcccgtccgg gcggtaagcg gccatgtcgg cgccggagcg cgtcagcatc
     3481 tcgcgcacca gcgacgagtc ccacgcgcgc gccatcaacg cggtcttggc caccggcggc
     3541 gtcggcgccg ccttgatgat ggcgatgaac tcgtcgatgt tggccagcgc cacggcgagg
     3601 ccttccagca cgtggccgcg cttgcgcgcc tccttgagtt caaaaaccgt gcgccgcgtg
     3661 accacctcgc gccgatgcga caggaacgca tcgagcatct gcttgaggtt cagcagccgc
     3721 ggctggccat cgaccagtgc gaccatgttg atgccgaacg tatcctgcag ttgcgtctgt
     3781 ttgtagaggt tgttgagcac cacctccggc acttcgttgc gcttgagctc gaacacgacg
     3841 cgcatgcccg acttgtcaga ctcgtcgcgg atgtcggaga tgccctcgag ccgcttctcg
     3901 ttgaccatct cggcgatgcg ctcgagcaac acgcgcttat tcacctggta gggcaactcg
     3961 tcgacgatga tcgcgctgcg cgctcccttg tcgatttcct cgaagtgcgt cttggcgcgc
     4021 atgacgacgc ggccacggcc tgtgcgatag ccttcgcgca cgccttcgag gccgtagatg
     4081 atcccggcgg tcgggaagtc cggcgcggga atgagttcaa tgagttcgtc caccgtcgtc
     4141 tcggaccggc gcagcagcac caggcagccg tcgattacct ccgagaggtt gtgcggcggc
     4201 acattggtcg ccatgccgac cgcgatgcca gccgagccat tgatcagcag gttagggatc
     4261 ttcgacggca gcaccgacgg ctcgcgctcc ttgccgtcgt agttcgggac gaagttgacc
     4321 gtttccttgt cgatgtcggc cagcaactca ccggcgatct tgtcgagccg gcactcggtg
     4381 tagcgcatcg ctgccgcgtt gtcgccgtcg atcgagccga agttgccctg tccatcgatc
     4441 agcgtgtagc gcagcgagaa gtcctgcgcc atccgtacca gcgtgtcgta aatggcgttg
     4501 tcgccgtgcg ggtggtactt gcccatcacc tcgccgacca cgcgcgcgca cttcacgtag
     4561 gagcggttcc agtggttgtt cgactcgtgc atcgcgaaga gcacccggcg gtgcacgggc
     4621 ttcaggccgt cgcgcacatc gggcagcaca cgcccgacga tcacgctcat cgcgtaatcc
     4681 aaatacgaac gccgcatctc ctcttcgagc gagattggca gggtttcctt ggcgaattgg
     4741 tccatcaggg gcggggccgt cggtgcgagc ggttgagtca cgatcgctct gaaatgcaat
     4801 cgcaaattct accagtgcgg acgcccgatt tcgcccggac cggacagcgt ccgaggccgg
     4861 agtccccttg agcgcacagc ttcgccgcag tcacccgact cgccaatcgg cgcgcgatgt
     4921 gctttgatcc gccgccacac ttcgaaccgc ctgctcaaca tggaatccgt cgatctggtg
     4981 atcgaggcgc gctgggtcgt gcccgtcgtg ccgaagggcg ccgtccttga ggatcacgcc
     5041 gtcgtcgttc atgaaggccg catccttgcc ctgttgcccc gggatgctgc gcaggcgcgc
     5101 tacgagccgg tagagcgtgt ggtccttgcg acccacgtcc ttaccccggg actcgtcaac
     5161 ctccacaccc acgccgcgat ggctctgttt cgcggcatcg gcgacgatct gccgctgatg
     5221 cagtggctgc agcaacgcat ctggccgctg gagaaggcac tggtgtctcc ggagttcgtt
     5281 tacgacggca cgcgcatcgc agcgctggag atgctgcgct ccggcacgac ctgctgctcc
     5341 gacatgtact tctatcctga agccgccgtt cgcgcgctgc gcggcgtcgg catgcgcgca
     5401 gttgccggga tcatcgcaat cgagtttcct accgcctacg cggccgatgc cgaggactac
     5461 ctgcgcaagg gcctcgcgac gcgcgacgcc tgtcgcgacg acgcgctggt gagcttcacg
     5521 ctcgcaccac atgccccgta cacagtcgag gacgccacgc tgacaaagat cgccgtactc
     5581 gccgaagagc tcgatctgcc ggtgcacatc catctgcacg aaacggctga tgaggtcgct
     5641 gaatcagtac gccggtatgg ccaacgcccg ctcgcacgac tcgaccggct cggactggtg
     5701 accgaacgac tgatcgccag gtgctcgaca tcaacctcac cggcgtcttc ctttgcaaca
     5761 aggcagtcgc gccgcacatg cagcagaac
//
LOCUS       DATIRT010000434         1737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_170668, whole genome shotgun sequence.
ACCESSION   DATIRT010000434 DATIRT010000000
VERSION     DATIRT010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170668"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(328..1209)
                     /locus_tag="VLN25_09795"
     CDS             complement(328..1209)
                     /locus_tag="VLN25_09795"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HSQ10888.1"
                     /translation="MTSGSSSTHTSVPLLVSREMPGGAFRVSKDATFMKAFEEAGFHT
                     YWLAVQERGIAWPDANTEMFVTRKKTDRESLLPSLRLVLAQNAPKKLILVHAYGAHFS
                     YCDRYEPATAAFQADCSKLSELPDDSTLPDFIRAYDNAVAESLRFVDAVISELGQAGG
                     SSFVMYTADHGENLLDDGRKLFQHALKDPTRWDTTVPAVMWASDEWRASNSARWERLR
                     ANHEATAMHADLVPTLLGSAAIRYVDERQVAVDLGAMSPAADRTRWVMRRLGEKVDGD
                     QLRQVHPRGALGAGTGK"
     gene            complement(1255..>1737)
                     /locus_tag="VLN25_09800"
     CDS             complement(1255..>1737)
                     /locus_tag="VLN25_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10889.1"
                     /translation="AAGADLLRDVNLLDAALIRPDPHEAWPVIQPHAHWIALTAVLLA
                     VPIWVLFRTPSTRTARGKWPSATLAAITFAAVTCGAVFHDTVLRAWPLNVISIAAATA
                     IGRNEFISTALPYAPVNPRSASASWGVSRLPGLHPQNETYVLIIGESVRADRLGACGG
                     "
BASE COUNT          346 a          516 c          541 g          334 t
ORIGIN      
        1 tgcgtagccg acgcggtagc cgacgtcacc gacgtcgtgg gctggcacgt tggccgttcg
       61 gatggcgtgc tcaatcgaat cgatccgaag gtaatcagcg cccagcgatt cggacaagcg
      121 acgggcgaga gttgacttcc ctgcgcccgg caaacctgca aagacgacga gcacggcgca
      181 ccttcttgag aagtaaccac gccatcatat ttacgcagag cccgaacggc cgctatcggg
      241 ccaactgggg ctaccgcagc gacggccggt gatgccgaat gcggccgatg cgccgttttg
      301 tgcggctgcg gtgaccctta ggatttctca ttttccggtc cccgcgccta atgcgccccg
      361 gggatgtact tggcgcagtt gatcgccatc aaccttctca ccgagacgtc gcattaccca
      421 ccgggttcta tcggcagccg gtgacatcgc tccgagatcg acagccactt gccgctcgtc
      481 gacatatcta atggccgctg aaccgagcag ggtgggcaca aggtcggcat gcatcgccgt
      541 ggcttcatgg ttggcacgga gccgttccca acgcgcggag ttgctggcgc gccattcatc
      601 gctcgcccac attacggccg ggactgtcgt gtcccagcgc gtcggatcct tcagcgcgtg
      661 ttgaaaaagt tttcgtccgt cgtcaagcag gttctcacca tggtcagccg tgtacatcac
      721 gaaggacgag ccgcccgcct gccctagctc tgagatcaca gcatcgacga atcggaggga
      781 ttcggctacg gcgttgtcgt aggcgcggat gaaatccggc agggtggaat catctgggag
      841 ttcagaaagc ttcgagcagt cggcctggaa agcagctgtt gcgggctcat agcgatcgca
      901 gtacgaaaag tgtgcgccgt aggcatgcac caagatcaat ttcttgggcg cgttctgcgc
      961 gagcaccaat ctgagcgatg gcagaaggct ttctcgatcg gtcttctttc tcgttacgaa
     1021 catttcggtg ttggcgtcag gccacgcaat acctctttcc tggacggcaa gccaataggt
     1081 gtgaaatcca gcttcctcga aagccttcat aaatgttgcg tccttgctga cccgaaatgc
     1141 accgccaggc atttcgcgcg agacgagcag agggaccgac gtatgcgtgc tgctggatcc
     1201 ggacgtcaca tcgcaataga tgatcgcgtc ttgatggctg atggcgacct gcttttagcc
     1261 cccgcatgcg ccgagccggt cggcacgcac tgattccccg atgatgagga cgtatgtctc
     1321 gttctgcggg tggagtcccg gcaaccgcga gacgccccaa ctcgcggacg ccgaacgagg
     1381 gttaacgggc gcatagggca acgccgtcga gatgaattcg ttacggccga tcgcagtagc
     1441 cgcggcaatg gagatcacat tcagcggcca cgcgcgcagc acggtgtcat gaaagacggc
     1501 gccgcatgtg actgctgcga aagtgattgc cgcaagggtg gccgaaggcc acttcccgcg
     1561 cgccgtccgg gttgacggag tgcgaaacaa cacccagatc ggcacggcga gcaggaccgc
     1621 ggtcagggct atccagtggg catgaggctg aattactggc cacgcctcgt gtgggtccgg
     1681 ccgaattaag gccgcgtcga gaaggttgac atcgcgtaga aggtcggctc ccgcggc
//
LOCUS       DATIRT010000435         4538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_72728, whole genome shotgun sequence.
ACCESSION   DATIRT010000435 DATIRT010000000
VERSION     DATIRT010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72728"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VLN25_09805"
     CDS             <1..344
                     /locus_tag="VLN25_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HSQ10890.1"
                     /translation="YSRRPGIPAADLADETPQPVQLDRLQRLLALLNENVARYSSAMI
                     GTTRRILVEGPSRKDGEELMGRTENNRIVNFKGPTRLIVQMVDIVVTEALPHSLRGDV
                     RVREPADAPVA"
     gene            365..3172
                     /locus_tag="VLN25_09810"
     CDS             365..3172
                     /locus_tag="VLN25_09810"
                     /inference="COORDINATES: protein motif:HMM:NF027637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HSQ10891.1"
                     /translation="MNCISLPRMCGESKVRYLLAFACAALLSACATMPTGTAPAAAGA
                     AAGKGAVTTTTAGAATPTRPTGTAAGAPAPSVAVPGAPRPFAEVIKDAKPINGLFTVW
                     QKDDKTWIEIPPELMDQPFFLSIGLTRGLGERWLIGGLQGGSHTTGGEYIGQFRKAAG
                     NVQLLARNTRFVARSGSPEERAVRNAYADSLLASAPIVSAPHPERKSVLIEATALLLN
                     DIPRASHVIEREYRNSFSFDPRNSFIRSAKADAERTVFDITAHYAQPRIPLPAPPLPV
                     PGAMPMPFYPAPDLLEDPRSLFLGFLYTFSKLPDKPMAARAADSRIGHFTTSKFDFSS
                     ESRLSPMRHYVQRWRLEKKDPQAAVSEPVKPITYWLSNEIPEKYRTPIRDGVLEWNKA
                     FEKLGFKDAVVVRQQPDDSDVDLLETGTSSIRWQITARPSYGAIGPSHVDPRTGEILD
                     ADLGWDANFVRSARFLRSEEIGYRSVYDEASGDIAVEPSGIGSGAHCMLRELAMRELG
                     YTLALLEVRGEIEPDSPQAEAFIDDFIRWVTMHEVGHTLGLTHNFRASTINTPKQLAD
                     VLYTSRTGLIGSVMDYPPANIALKGEVQGQYYASALGAYDYWAIEYAYKSIAPEQEAT
                     ELATIAARASDPQLAFATDADAAAAIDPTANIWDLGSDPLAFYRKRALLTQELWQRLE
                     SRTMMPGESYAVLRRRFLSGLSQSALAMTQAAKYVGGAEIQNDAAGSPRLPITPTSAA
                     QQREALAVITAGLFKSDSFKVSPQFLRKLASDRLEREELPFTQQVSMFEIALPDRVLA
                     VQRDVLNRLMGPVVARRVLVNAEMAGKGSDAFTLSELYSTLQLAIWAEARNDRDATVL
                     RRNLQREHLRRITGAILGPSAGYPADARALMRQNARQLHGWLVAAAARPGLSAETRAH
                     FAEAAETLGEALKAPLIRTGV"
     gene            3233..4216
                     /locus_tag="VLN25_09815"
     CDS             3233..4216
                     /locus_tag="VLN25_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HSQ10892.1"
                     /translation="MDNDDLANVIGPLDANLRQVETELDVAIARRGHQFRVEGGADSA
                     QAAVTVLEYFLDRARRTGRPVSVDDMQLYFVEQRGQRGERAEPANEGDGPPQLHTRRS
                     DLHGRTPRQRDYITAMLAHDITFGIGPAGTGKTYLAVAAAVDALERDVVKRIVLTRPA
                     VEAGERLGFLPGDLAQKVDPYLRPLYDALFDLLGFDKTQRLLERQTIEIAPLAYMRGR
                     TLNQAFVILDEAQNTTREQMKMFLTRIGFGSKAVVTGDVTQIDLPRGTASGLIEAQHV
                     LAEVRGMAFTYFTAADVVRHPLVGRIVEAYEAAEARAEASDDDSASRRRRT"
     gene            4231..>4538
                     /gene="ybeY"
                     /locus_tag="VLN25_09820"
     CDS             4231..>4538
                     /gene="ybeY"
                     /locus_tag="VLN25_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HSQ10893.1"
                     /translation="MAIQGRARFDKLPARATLRRWISAALDRDAEITLRFVDAREGRK
                     LNRDFRGRDYATDVLTFDYAHTPVVRADIILCPPVVKRAARERRRLFRDHLAHLVVHG
                     T"
BASE COUNT          811 a         1510 c         1493 g          724 t
ORIGIN      
        1 tctacagccg acgccccggc ataccggcgg cggatctggc ggacgagact ccacagcccg
       61 tccagctgga ccggctgcag cgcctgctgg cgctcctgaa cgagaacgtt gcgcgttaca
      121 gcagcgcgat gatcggcacg acccggcgca tcctggtcga aggtccgtcg cggaaggatg
      181 gcgaggaact gatggggcgc accgagaaca atcgcatcgt gaacttcaag ggccccacgc
      241 gactcatagt ccagatggtc gacatcgtcg tgaccgaggc gttgccgcat tcgctgcgcg
      301 gcgatgtgcg ggtgcgcgag ccggccgacg ccccggtcgc gtgaatcgag cagggagttt
      361 tccgatgaat tgcatttcgc tgccgcggat gtgcggcgaa tcgaaggttc gctacctgct
      421 ggcattcgct tgcgccgcgc tgctctcggc ctgcgcaacg atgccgaccg ggactgcacc
      481 tgccgcggcg ggtgcggcgg cgggcaaggg tgcagtaacg acgacaaccg cgggagcggc
      541 gacgcccacg cggcccaccg gtaccgcggc cggcgcccct gcaccgtcag tggcagttcc
      601 cggcgcgcca cgcccgttcg ccgaagtgat caaggacgcg aagccgatca acgggctttt
      661 tacggtctgg cagaaggacg acaagacgtg gatcgagatt cccccggaac tgatggacca
      721 gccgttcttc ctgtcgatcg gtctgacgcg cggacttggc gagcgctggc tgatcggcgg
      781 tctgcagggc ggcagtcaca cgaccggcgg cgaatacatc ggccagttcc gcaaggcggc
      841 gggcaatgtg cagttgctcg cacgaaacac gcgcttcgtg gcgcgctccg gttcgcctga
      901 ggaaagggct gtccgcaacg cctatgcgga tagcctgctg gcctcggcgc cgatcgtcag
      961 tgcgccgcat ccggagcgca agtcggtgct gatcgaggca acggcgctgc tgctcaacga
     1021 catcccacgt gcttcgcatg tcatcgagcg cgagtaccgc aacagcttct cttttgatcc
     1081 gcgcaactcg ttcattcgat cggccaaggc cgatgccgag cgcacggtgt tcgacatcac
     1141 ggcccactac gcgcagccgc gcatcccgct gccggcgccg ccgttacccg tgccgggtgc
     1201 catgccgatg cctttttatc cggcaccgga cctgctcgag gatccgcgca gcctgttcct
     1261 tggcttcctc tacacgttca gcaagctgcc ggacaagccc atggcagcgc gcgccgccga
     1321 ttcgcgcatc gggcacttca cgacgtcgaa gttcgatttc agttcggagt ccaggctttc
     1381 gcccatgcgc cactacgtgc aacgctggcg gctcgaaaag aaggatccgc aggcggccgt
     1441 atcggagccc gtcaagccga tcacgtactg gctgtcgaac gagattccgg agaagtaccg
     1501 cacgccgatc cgcgacggcg tcctcgagtg gaacaaggcg ttcgaaaagc tcggcttcaa
     1561 ggacgcggtg gtcgtcaggc aacagcccga tgactcggac gtcgatctgc tggaaaccgg
     1621 gacctcgtcg atccgctggc agatcacggc ccgtccatcg tacggagcca tcggtccgtc
     1681 acacgtcgat cctcggaccg gagagattct ggacgcggat ctcggatggg atgccaactt
     1741 cgtgcgcagt gcgcgcttcc tgcgcagcga ggagatcggc tacaggagcg tgtacgacga
     1801 agccagcggc gacatcgcgg tcgagccgtc gggcatcggc tccggtgcgc attgcatgtt
     1861 gcgtgagcta gcgatgcgag agctcggcta tacgctggcg ctgctcgaag tccgcggcga
     1921 aatcgagccc gacagcccgc aggccgaggc cttcatcgat gacttcatca ggtgggtgac
     1981 gatgcacgaa gtcggccaca cgctcggact cacgcacaac ttccgcgcat ccaccatcaa
     2041 tacgccgaag caactggccg atgtgctgta cacgtcgagg acaggactga tcggctccgt
     2101 gatggactat ccgccggcga acatcgcgtt gaagggtgaa gtgcaggggc agtactacgc
     2161 gtccgcgctt ggggcctacg actactgggc gatcgagtac gcctacaagt ccatcgctcc
     2221 ggagcaggag gcgaccgaac tggcaacgat cgctgcgcgc gccagcgacc cgcaactcgc
     2281 gtttgccacg gatgcggatg ccgcggccgc gatcgacccg acagcgaaca tctgggatct
     2341 cggatcggat ccgctggcgt tctatcgcaa gcgggcgttg ctcacgcagg aactgtggca
     2401 acggctggaa agccgcacga tgatgcccgg agagagctac gccgtgctgc gacggcgctt
     2461 cctttccgga ctgagccagt cggcgctggc gatgacccag gcggcgaagt acgtcggggg
     2521 cgcagagatc cagaacgacg ccgccggcag cccgcggctg ccgatcacgc cgacctccgc
     2581 cgcgcaacag cgcgaagcgc tcgccgtcat caccgccggc ctgttcaaga gcgacagctt
     2641 caaggtttcg ccccagttcc tgcgcaaact cgcaagcgac cggctggagc gggaggagtt
     2701 gccgttcacg caacaggtgt cgatgttcga gattgcactg cctgaccggg tcctggcggt
     2761 gcaacgcgat gtcctcaatc gcctgatggg accggtggtc gctcggcgag tgctggtgaa
     2821 cgcggaaatg gccggcaagg gatccgatgc cttcaccctg tccgagcttt actcgacgct
     2881 gcaactggcc atctgggcgg aagcgcgcaa tgacagggat gcgacggtcc tgcggcgcaa
     2941 cctgcagcgc gagcacctgc gccgcatcac cggcgccatc ctgggaccgt cggcgggata
     3001 ccccgccgat gcccgcgcgc tgatgcgcca gaacgcgcgc cagctgcacg gatggctggt
     3061 cgctgcggcg gcccggccgg gcctctcggc cgagactcgc gcgcactttg cagaagctgc
     3121 cgagacgctg ggcgaagcgc tgaaggcgcc gctgatccgt accggggtct aaactcatcg
     3181 cgtggcacgg cgcggttcaa catcaaaaaa ggtcctgacg ttcacttcgg acgtggacaa
     3241 cgacgacctc gccaacgtca tcggtccgct cgatgcaaac ctgcggcagg tcgaaacgga
     3301 actcgacgtg gcgatcgctc gccgcggtca ccagttccgc gtggaaggcg gcgctgacag
     3361 cgcgcaggca gcggtcacgg tgcttgagta ctttctcgat cgtgcacgcc gcaccggccg
     3421 gccggtatcg gtcgacgaca tgcagctgta cttcgtcgag cagcgcggcc agcgcggcga
     3481 gcgcgcggaa ccggctaacg aaggcgatgg accgccgcag ctgcacacgc gccgcagtga
     3541 cctgcatggc cgtacgccgc gccagcgcga ctacatcacc gcgatgctgg cgcacgacat
     3601 caccttcggc atcggcccgg caggcaccgg caagacctac ctcgcggtgg ccgcggccgt
     3661 cgatgcgctc gaacgcgatg tcgtcaagcg catcgtgctg actcggccgg cagtggaagc
     3721 cggcgagcga ctgggcttcc tgccgggcga cctggcccag aaagtcgatc cgtacctgcg
     3781 accgctgtac gacgccttgt tcgaccttct cggcttcgac aagacccaac gcctactcga
     3841 gcgccagacg atcgagatcg cgccgctcgc ctacatgcgg gggcgcacgc tgaaccaggc
     3901 tttcgtcatc ctcgacgagg cgcagaacac gacgcgcgag cagatgaaaa tgtttctgac
     3961 gcgcattggc ttcggctcca aggcggtcgt caccggcgac gtcacgcaga tcgacctgcc
     4021 gcgcggtacg gcgagcggac tgatcgaggc gcagcatgtt ctggccgagg ttcgcggcat
     4081 ggccttcacg tatttcaccg cagccgatgt ggtgcggcat ccgctcgtgg gacgcatcgt
     4141 cgaagcctac gaggctgccg aggcgcgtgc cgaagccagc gatgacgaca gcgcgtcgcg
     4201 tcgccgccgc acgtgacccc cgcgctcgac ctggcgattc agggccgcgc tcggttcgac
     4261 aaactgccgg cccgggccac gctcaggcgc tggatcagtg cggcactcga ccgcgacgcg
     4321 gaaatcacac tgcgtttcgt tgacgcacgc gagggtcgga agctgaaccg cgattttcgc
     4381 ggcagggact acgccaccga cgtgttgacg ttcgactatg cccacacgcc cgtggtccgg
     4441 gccgacatca tcctttgccc gccggtggtg aagcgcgcag cgcgcgaacg gcgacggctg
     4501 tttcgcgacc acctcgcgca cctcgtcgtg cacgggac
//
LOCUS       DATIRT010000436         4643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1254150, whole genome shotgun sequence.
ACCESSION   DATIRT010000436 DATIRT010000000
VERSION     DATIRT010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254150"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VLN25_09825"
     CDS             complement(<1..468)
                     /locus_tag="VLN25_09825"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HSQ10894.1"
                     /translation="MMDTLDTPVRLGAFAASHTDLAALQRGPVLARLDDLGCIRAEGP
                     DALTFLQSQLTNDVAHLAPAALQLNAYCTPKGRLLATFHQWRDGEAVLLQLPREILVP
                     VMKRLSMFVLRSKARLIDASTQHAAYGLIGPQAADALRADGLVVPDAPWMSTTH"
     gene            535..1563
                     /gene="mltG"
                     /locus_tag="VLN25_09830"
     CDS             535..1563
                     /gene="mltG"
                     /locus_tag="VLN25_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HSQ10895.1"
                     /translation="MAKSSRTRALTLAAVVVFALGAAAALGARWYVNERPLPMRPMSA
                     DAIEFRVKPGTSARGVAQAARAAGIEVNELAFAALARGTDTAQALRAGRYSIARGTTM
                     AQLMAKLQAGEVLRERLTIVEGITFVEMRAVLAAAADLRQDSANMTPAQLLQAIGASE
                     SHPEGLFAPDTYLYDPGSSDLDVWRRAYRAQMATLQAAWESRAADLPYRSPYEALIMA
                     SIIEKETGQASERPMIGGVFVNRLRRGMLLQTDPTVIYGLGERFDGNLRKRDLVTDGP
                     YNSYTRAGLPPSPIALPGRAAIESALRPAATEALYFVARGDGTSQFSASLDAHNRAVD
                     KYQRGIGR"
     gene            1560..2177
                     /gene="tmk"
                     /locus_tag="VLN25_09835"
     CDS             1560..2177
                     /gene="tmk"
                     /locus_tag="VLN25_09835"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HSQ10896.1"
                     /translation="MTERGRFITVEGIDGAGKSTQIDAVAAALRARGLHLVLTREPGG
                     TSLGESLRELILNERMTAETETLLLFAARAEHLDRVIRPALAVGQWVLCDRFTDATYA
                     YQSGGRGVAVERIAALEHWVHADLQPDLTLLFDVPPEVAAQRLSQARTADRFEAEDVH
                     FFAAVRGAYLARAASDPARFFVIDGTRPPEVLRAQLTELAEKWNG"
     gene            2165..3226
                     /gene="holB"
                     /locus_tag="VLN25_09840"
     CDS             2165..3226
                     /gene="holB"
                     /locus_tag="VLN25_09840"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HSQ10897.1"
                     /translation="MERLTPLPWQGALWQQVAAGRERMPHALLLHGARGIGKRHFAHA
                     LAQSLLCEAPEEGPALACGQCSGCQLLAADNHPDLRWLVPEVDQPMRDELDDEQDDGA
                     TPGSSKSTKPSRAILIDQVRAIGEFLALAAHRGGRRVVILAPAEALNGPAANALLKLL
                     EEPPPGAVFIAVTDELDAVLPTVRSRCVLLRAPVPETAVALTWLRAQGVEDAEAKLAE
                     AGGAPVGLEVVDEDARRLAPDLKARLLDLLTRGGRLSLSEVVSAVPKDIPVPAAIRLF
                     QCWGWDLLAERAAGRVRYHPRHQRAIASVARACEPERLLAWLGRLDDAQATAEHPLNA
                     RLVVESALIGYLDAVRPAR"
     gene            3249..3632
                     /locus_tag="VLN25_09845"
     CDS             3249..3632
                     /locus_tag="VLN25_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSQ10898.1"
                     /translation="MNDAAVKSAANGQPGGARPAVLSLSIKEKAALYAAYMPYLKNGG
                     IFVPTTRPYQLGDDVYLILTLLDDSTRIPVAGKVVWVSPAAGGNKTPGIGVHFPPDES
                     GLTARKRIEDALGAALKSARPTHTL"
     gene            3676..4461
                     /locus_tag="VLN25_09850"
     CDS             3676..4461
                     /locus_tag="VLN25_09850"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HSQ10899.1"
                     /translation="MFVDSHCHLSFPELAGDLAGILERMKAARVEAALNVCTTLEEFP
                     RVLATARSAGNLVASVGVHPDTTGTPEPTADLLLELADDPKVVAIGETGLDYYRLPEP
                     LDWQRERFRIHIRAARACGKPLIIHTRAAAEDTLRLMREEGADEVGGVMHCFTETKDV
                     ARQAMDLGFHISFSGIVTFKNAKELQAAAAGVPLDRLLIETDAPYLAPVPYRGRTNEP
                     SYVPHVAVKLAALHGLEVAEMAAATTGNFYRLFNVLHDSFKVN"
     gene            complement(4518..>4643)
                     /locus_tag="VLN25_09855"
     CDS             complement(4518..>4643)
                     /locus_tag="VLN25_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSQ10900.1"
                     /translation="RVMERLGMRRNAREDFDHPRVPQGHPLQRHVLYRLAARDFK"
BASE COUNT          784 a         1488 c         1575 g          796 t
ORIGIN      
        1 atgagtcgtc gacatccagg gtgcatccgg cacaaccagt ccgtcagctc gcagcgcgtc
       61 ggccgcctgc ggtccgatca ggccgtacgc ggcatgctgg gtgctggcat cgatcagccg
      121 cgccttggag cgcagcacga acatcgacag ccgcttcatc acaggcacca ggatctcgcg
      181 cggcaactgc agcaacacgg cctcgccgtc gcgccactga tgaaacgtgg cgagcaagcg
      241 acctttcggc gtgcaatacg cattgagctg cagcgccgcg ggtgcgagat gtgcaacgtc
      301 gttggtcagc tgggactgca gaaaagtgag cgcgtcgggc ccctcggccc ggatgcatcc
      361 gagatcgtcg agtcgcgcga gcaccggacc gcgttgcagg gccgcgagat ccgtgtgcga
      421 cgccgcgaag gcgccgagtc ggacgggggt atcgagggtg tccatcatca gttcacgaag
      481 gcagattcag catgccgatt atcataggtg tcgttcgtcg caaagtctca tggaatggcg
      541 aaatcttcgc gcacgcgggc gctgaccctt gccgcagtgg tggtgttcgc gctgggcgct
      601 gcggcggcgc tcggcgcgcg ctggtacgtc aatgaacggc cactgccgat gagaccgatg
      661 agcgcggacg cgatcgagtt tcgcgtcaag ccaggtacca gcgcgcgcgg cgtggcacag
      721 gcggctcgcg cggccgggat cgaggtcaac gaacttgcct tcgcggcgct ggcacgtggg
      781 accgacacag cgcaggcgct gcgtgcgggc cggtactcga tcgcgcgagg tacgacgatg
      841 gcacagctga tggccaagct gcaggcagga gaggtcctgc gggaacgact cacgatcgtt
      901 gagggcatca ccttcgtcga gatgcgcgcg gtgctcgcgg ccgctgcgga cctgcggcag
      961 gacagcgcga acatgacgcc ggcccagctg ctgcaggcga ttggcgcatc cgagtcgcac
     1021 ccggaaggcc tgttcgcccc ggacacgtat ctttacgatc ccggcagcag cgatctcgac
     1081 gtctggcgac gcgcctatcg tgcgcagatg gccacgctgc aggcagcgtg ggagtcacgc
     1141 gcggcggacc tgccgtaccg ctcgccctat gaggcgctga tcatggcctc gattatcgag
     1201 aaagagaccg gtcaggcgag cgaacggccg atgatcggcg gtgttttcgt caaccggctc
     1261 aggcgcggca tgctgttgca gaccgatccg accgtcatct atgggctggg cgagcgcttc
     1321 gacggcaacc tgcgcaagcg cgatctcgtg accgatggtc cgtacaactc gtacacccgt
     1381 gcaggcctgc cgccgtcccc gatagcgctg cccggacgcg cggcgatcga atccgcactg
     1441 aggcccgcag cgaccgaggc actgtacttc gtcgcgcgcg gtgacggaac gtcccagttc
     1501 tcggcctcgc tggacgcgca caatcgtgca gtcgacaagt atcagcgggg catcggaaga
     1561 tgaccgagcg cgggcgcttc atcaccgttg aaggcatcga tggcgccggc aagagcacgc
     1621 agatcgacgc ggtcgccgcg gcgctgcgtg cgcgtggtct gcatctcgtg ctgacccgcg
     1681 aaccgggcgg cacctcgctc ggcgaatcgc tgcgcgagct gatcctcaac gagcgcatga
     1741 ccgccgagac cgagacgctg ctgctctttg ctgcgcgcgc cgagcacctc gaccgtgtca
     1801 tccggccggc acttgcggtc gggcagtggg tcctgtgtga tcgctttacc gacgccacct
     1861 acgcctacca gtcgggagga cgtggcgtgg ccgtggagcg cattgccgcg ctcgagcact
     1921 gggtgcacgc tgatctgcag ccggacctga cgctgctatt cgatgtgcca ccagaggtcg
     1981 ctgcccaacg cctgtcgcag gcacgcacgg cggatcgctt cgaggccgag gatgtccact
     2041 tcttcgcagc ggtacgcggt gcgtatctcg ctcgtgccgc gtcggatcct gcccgcttct
     2101 ttgtcatcga cgggacgcgt ccgcccgaag tgttgcgcgc gcaattgact gaactggctg
     2161 agaaatggaa cggttgacgc cactgccctg gcagggcgcg ctctggcagc aggtcgccgc
     2221 cgggcgcgag cgcatgccgc acgcgctgct gttgcacggc gcccgcggca tcggcaagcg
     2281 gcacttcgcg cacgcactgg cgcagtcgct cctgtgcgag gcgccggaag aagggccggc
     2341 gctggcgtgc ggacagtgtt ccggctgcca gttgctggcg gccgacaatc atccggacct
     2401 gcgctggctc gtcccggagg tcgaccagcc gatgcgcgac gagctcgacg acgagcagga
     2461 cgacggcgcg acgccaggtt cttcgaaatc caccaagccg agccgtgcca tcctcatcga
     2521 tcaagtgcgc gcgatcggtg agttcctcgc cctggcggcg catcgtggcg ggcggcgcgt
     2581 cgtcatcctg gctcctgccg aggcgctgaa cgggccggcc gcgaatgctc tgctgaagct
     2641 gttggaggag cctccgcccg gggctgtttt catcgcggtg acggacgaac tcgatgctgt
     2701 attgccgacc gttcgttccc gctgcgtcct gctgcgtgcg cccgtaccgg agacagcagt
     2761 ggcgctgacc tggctgcgag cccagggtgt cgaggatgcc gaggcgaaac tggccgaagc
     2821 gggcggggcg ccggtgggtc tggaggtcgt ggatgaggat gcgcgccggc tggcccccga
     2881 cttgaaggcg cgcctgttgg acctgctgac acggggaggc cgactgagcc tctcagaggt
     2941 ggtctcggcg gtgccgaaag acataccggt gcctgcggcg atccgtctgt ttcagtgctg
     3001 gggatgggac ttattggcgg aaagggccgc aggacgcgtt cgctatcatc cgcggcatca
     3061 gcgagcgatc gcgtcagtag cccgtgcctg cgagccggag cggctgcttg cctggctcgg
     3121 tcggctggac gatgcgcagg ccacggccga gcacccgctg aacgcgcggt tggtggttga
     3181 aagtgcgctg atcggctatc tggacgcggt ccgtccggcg cggtaagtaa cgagggatac
     3241 aacgcctgat gaacgatgca gcagtgaagt cggcagccaa cgggcaaccg gggggtgcac
     3301 gtcccgcggt gctgtccttg tcgatcaagg aaaaggccgc cctgtacgct gcttacatgc
     3361 cgtaccttaa gaatggcggt atcttcgttc ccaccacgcg tccctatcag ctgggtgacg
     3421 atgtgtacct gatcctgacg ctgctcgatg actcgactcg tattccagtg gccggcaagg
     3481 ttgtctgggt cagtcctgcg gcgggcggca acaagacacc gggcatcggc gtgcactttc
     3541 cgcccgacga gtcgggcctg accgctcgca agcggattga agacgccctg ggcgcggcgt
     3601 tgaagtcggc ccgtccgacc cacaccctgt agtctttggc ctgctgcctc cgtccgagcg
     3661 ggcggaggtg tttccatgtt cgtcgactcc cactgccacc tgtcgtttcc ggaactcgcc
     3721 ggcgatcttg cgggcatcct ggaacgcatg aaggctgcgc gggtcgaggc ggcgctcaac
     3781 gtctgcacga cgctcgagga attcccgcga gtgctggcca cggcgcgatc ggctggcaat
     3841 ctcgtcgcgt cggtcggcgt gcacccggac accacgggga cgcccgagcc caccgcggat
     3901 ctcctcctcg aactcgccga cgatccgaaa gtcgtggcca ttggggagac cgggctcgac
     3961 tactaccggt tgcccgagcc cctggactgg cagcgagagc gcttccggat ccacatccgc
     4021 gcggcacggg cctgcggcaa gccgctgatc attcatacgc gagccgccgc tgaggacacg
     4081 ctgcgactga tgcgggagga aggtgccgat gaggtgggcg gcgtcatgca ctgctttacc
     4141 gaaaccaaag acgtcgcgcg ccaagccatg gacctgggct tccacatctc gttctcgggc
     4201 atcgtgacct tcaagaacgc gaaggagctg caggccgcgg cagctggcgt gccgctggac
     4261 cgcttgctga tcgagaccga cgcgccgtac ctggcgccgg ttccgtatcg aggccgaacg
     4321 aacgagccgt cgtacgtgcc gcatgttgca gtcaaactgg ccgcgctaca tggcctcgag
     4381 gtcgccgaaa tggcagccgc aaccacgggt aatttctatc gcttattcaa tgtattacat
     4441 gattctttta aagtaaatta gtaaataagc cggcaacctg gcgggttaaa cgcacccctc
     4501 cggcatgact tgagaattca cttgaagtcg cgagcggcca gtcgatacag gacatgtcgc
     4561 tgcaacgggt gcccttgcgg cacccgcgga tgatcaaaat cctctcgcgc gttgcgtcgc
     4621 atacccagcc gttccatcac ccg
//
LOCUS       DATIRT010000437         1948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_467445, whole genome shotgun sequence.
ACCESSION   DATIRT010000437 DATIRT010000000
VERSION     DATIRT010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467445"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(418..984)
                     /locus_tag="VLN25_09860"
     CDS             complement(418..984)
                     /locus_tag="VLN25_09860"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HSQ10901.1"
                     /translation="MLTSIRSVFNHPGMRRAYVRLRVPLAVVAIILAFPYLRRDWLVA
                     GFAVSMVGELIQLWSFASLDKNSDLAIRGPYAMVRNPMYLGRYFILLGFLMLLGTWWI
                     LVLYTVGYWFYMDTRVEREEAHLRPIFGPRYDEYCARVRRFMPGPPLPGQPVAYWNWT
                     LFRQNNAAANLLGTLAVWAAVAAWLLLR"
     gene            complement(1003..1671)
                     /locus_tag="VLN25_09865"
     CDS             complement(1003..1671)
                     /locus_tag="VLN25_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HSQ10902.1"
                     /translation="MSRFDRPAIDGARRTLLRDGRWANARVSLVDVDGRQWVVKDFAP
                     RSWWVRNSIGRLFVRRELATLRRLAGIDGVPPHAFRLDAHAISFGYIEGETLGRIDEA
                     RQTPAYFAALERLMRAIHARGVVHLDTRGTGNVLMRPDGSPAVIDFQSALSTRWMPAS
                     LRRFMENVDLTGVYKKWLQRDAASMGPERIALNERMTRWRRFWVLRGYAGARKSHGRA
                     DRAP"
BASE COUNT          354 a          642 c          656 g          296 t
ORIGIN      
        1 gtttgagatc aggaaaccgc ccggcgtgaa gttgggagcg cccgtgagtc ggggcattcc
       61 gaatgctgcc gcgcagccgg tcgggcgact atcccgtccg ggttcatccg gacgctgtat
      121 caaggacgag ggcctgttca cgctaaatgc gggagatgcg ttgggtcgca atgcggatgg
      181 atgcgcgaag ggtggcgcag cgcagggtag ctccctgcgc aagccgcccg acatagcaga
      241 cgccgcattt cggcccaacc ctgcgggaag gcgaatggcg ggcgcattgc ttcgttgccg
      301 cttcgctgat tggcatcgag ccaacggcgc tcgcggcgcc tcgctctgcc tcccgccatt
      361 ggccttcgca ctcccggctt tagcgcgaac aggccctcag tggccgctgc ggtcgcgtca
      421 gcgcagcaac aaccaggcag cgacggcggc ccagacggcc agcgtcccga gcaggttggc
      481 ggccgcattg ttctggcgga aaagcgtcca gttccagtag gcgaccggct gcccgggcag
      541 cggcggccca ggcatgaacc gacgcacgcg cgcgcagtac tcgtcgtagc gcggcccgaa
      601 aatgggccgc agatgcgctt cttcgcgctc gacccgcgtg tccatgtaga accagtaccc
      661 gaccgtgtag agcacgagga tccaccaggt cccgagcagc atcaggaagc cgagcaggat
      721 gaagtagcgc cccaggtaca tcggattgcg caccatcgcg tacggcccgc ggatggccag
      781 gtcggaattc ttgtcgagcg acgcaaagct ccacagctgg atcagttcac ccaccatgga
      841 cactgcgaag cctgcgacga gccagtcgcg ccgcaagtac ggaaacgcca ggatgatggc
      901 gacgacggcg agcggcacgc gaagacgcac ataggcgcgg cgcatgccgg ggtggttgaa
      961 gacggagcgg atcgaggtca gcatgcgttc tgccgtcgcg gatcagggcg cacgatcggc
     1021 gcggccgtga gacttgcggg cgccagcata cccgcgcagc acccagaacc ggcgccagcg
     1081 ggtcatgcgt tcgttcaatg cgatgcgctc ggggcccatc gacgccgcgt cccgctgcag
     1141 ccacttcttg tagaccccgg tcaagtcgac gttctccatg aagcgccgca gggaggccgg
     1201 catccagcgc gttgacagcg ccgactggaa gtcgatcacc gccggcgaac catcgggccg
     1261 catcagcacg ttgccggtgc cgcgagtatc caggtgcacg acgccgcgcg catggatcgc
     1321 ccgcatcagt cgctcaagcg ccgcaaagta ggccggggtc tggcgcgcct cgtcgatccg
     1381 cccgagggtc tcgccttcga tgtagccgaa tgagattgca tgggcgtcga gccggaatgc
     1441 gtgcggcgga acaccgtcga tgcccgccag acgacgtagc gttgccagct cgcgccggac
     1501 gaaaagccgg ccgatgctgt tgcgcaccca ccaggaccgc ggcgcgaagt ccttgacgac
     1561 ccactggcgt ccgtcgacgt ccaccagcga cacacgggca ttggcccagc ggccgtcgcg
     1621 caacagcgtc ctgcgggcgc cgtcaatggc cggacgatcg aatcgcgaca agtcgttcct
     1681 caggtttggg ttaggcgggt ctgcgggggc cacgcatgcc gagacaaggg aatttatccc
     1741 atcctcaatg gcacctcgtc gacgcacgaa cggtcgccca ctggcccgct cacgtcgagg
     1801 cgcgaccgca cgtcgctttg gctggctgaa cggtagtccg aaaagctggc cgaatgcggc
     1861 gttgccccct aagatcggcc gccatgaacc tgctaatcat cggcggcggc gtgtttctgg
     1921 ggcaggcatt gctcggtgcc gcactgga
//
LOCUS       DATIRT010000438         5495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_958663, whole genome shotgun sequence.
ACCESSION   DATIRT010000438 DATIRT010000000
VERSION     DATIRT010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5495
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958663"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1258)
                     /locus_tag="VLN25_09870"
     CDS             complement(<1..1258)
                     /locus_tag="VLN25_09870"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HSQ10903.1"
                     /translation="MARPTIPIAPATQVEGGPRPTTAPPKAASRSLLLSPHRLAFFAG
                     GVSLLLASFAWAVWLFLSSAGVSVTTALPPTLAHGFVFAAAYMPLFIAGFMFTAGPRW
                     LDVPSPPATLLIWPVRLHVAGILLVLLGTVVSPGTAAFGSLLLAIAWTAVGVAFATLV
                     RSSRARDRLHARCVLAFWIAGIAAALLFAAGLATHQFGVVAAAVWLLVFACIAPITVT
                     VAHRVLPFFTSNAVTTLVPWRPNWVLGLLVAGILVFGGLQVAGRFDLTGAKSISWLTL
                     TVVAPISLALIALAIRWGLVQSLRGPSLRLLAMLHVAVVWIPLALALAAIDAAWVLLG
                     AGAPRLGLMPLHALTIGFLGSMLFAMATRVISGHVGLAVTADTFVWRLVWGLQAATLL
                     RLAAPLSPAASVGLPLASAALWTLIWT"
     gene            complement(1373..2068)
                     /locus_tag="VLN25_09875"
     CDS             complement(1373..2068)
                     /locus_tag="VLN25_09875"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HSQ10904.1"
                     /translation="MPDELLERLRRFKDDAFPQYRAQFQQLVDDGQRPLTLFIGCSDS
                     RIVPYLLTGAGPGELFLVRNVGGFVPPYDQSHGFHGTAAAIEFAVLSLDVKRIVVCGH
                     SHCGAIRALYGDVPPAAQNLVRWLELGREAVLPVAEPGPDVLRRTEQRAIVLQLERLM
                     DYPMVRERVEAGTLTLHGWHYVIEEGEVHVFDVQRGGFVPASQADNAGTGPYKLQEPA
                     LSLDASGVPAFGP"
     gene            complement(2086..2880)
                     /locus_tag="VLN25_09880"
     CDS             complement(2086..2880)
                     /locus_tag="VLN25_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSQ10905.1"
                     /translation="MLATACTPESTTAPRVNGLDLAAPDEVLSPNELRSRANVELLRQ
                     AAIASGLLGADDPAPAGGAISEAAADAIEALLERELVRPEADEPTCRRFHAANASRYA
                     VGEKVQLRHVLFAVPPGVDLDGLRKRAEACLIDLRARTHGEADRFPQAAAQLSNCPSG
                     QDGGQLGWLTAADCAPEFAREIFGQPEVGVLPRLVRSRFGLHVVEVLGREPGTVPTYE
                     DVKAAVRLTLDRQAYATALRQYLQLLAGRARVEGVDLLAASTPLVQ"
     gene            complement(2880..3605)
                     /gene="narI"
                     /locus_tag="VLN25_09885"
     CDS             complement(2880..3605)
                     /gene="narI"
                     /locus_tag="VLN25_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HSQ10906.1"
                     /translation="MTLLHNFFFNVYPYVCLAFFFMGSLARFDRDQYTWKSDSSQMLR
                     AKQLRWGSNLFHIGILFLLFGHTVGLLTPHWVYEPFITAPTKQLFAIVSGGIAGTMCF
                     IGLTMLLHRRMFDPRIRLTSHRTDLAILIILWIQLLLGLITLPLSYAHRVDAHAMLVL
                     SAYLQGVATFRPDANLLTEIAWPFKVHMVLGMTIFLLLPFSRLVHVWSGLASIAYLFR
                     PYLVVRSRRLNVPAGQNVPGRVS"
     gene            complement(3616..4314)
                     /gene="narJ"
                     /locus_tag="VLN25_09890"
     CDS             complement(3616..4314)
                     /gene="narJ"
                     /locus_tag="VLN25_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HSQ10907.1"
                     /translation="MKPFDSAPPKMTHTLRALAWLLRYPDAELRALLPQLRDALRAEA
                     ALDSGRLGELDALVSQLLGTAPLRTEAEYVEIFDRGRRTALHLFEHVHGDSRDRGPAM
                     VDLAQTYERAGLHLAPGELPDYLPAVLEFASTQPPTQAREFLREIAHVVRAIFSALLE
                     RQSPYASVLAAVLDLAGERAERVALPTEPAIDESWAEPEAFSGCSSQGQSRPNEAQPV
                     HIVRSARAPHRTAA"
     gene            complement(4317..>5495)
                     /gene="narH"
                     /locus_tag="VLN25_09895"
     CDS             complement(4317..>5495)
                     /gene="narH"
                     /locus_tag="VLN25_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HSQ10908.1"
                     /translation="EMKAAPTARPRSLITGQRMEKIEWGPNWEEILGGEFSKRSKDRN
                     FDDIQKEIYGQFENTFMMYLPRLCEHCLNPACVASCPSGSIYKREEDGIVLIDQDKCR
                     GWRMCVSGCPYKKIYYNWKSGKAEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEAASTEHDRNLYSAQLGIFLDPNDPRGIEQARADGIPEAWLAAAKTSPVYKMA
                     VDWRVALPLHPEYRTLPMVWYIPPLSPVTSRAQAGDIGMNGELPDVRSLRIPLKYLAN
                     LLTAGDEEPVARALERMMAMRVFMRSRHVDGVDKPAVLERVGLTRAEVEDMYRVMAIA
                     NYEDRFVIPTTHREYAENAFNVRGGCGFSFGNGCSEGVSETSLFGSEKKRTIPIKATV
                     "
BASE COUNT         1010 a         1757 c         1883 g          845 t
ORIGIN      
        1 cggtccagat cagcgtccac agtgcagcgg atgcgagcgg cagtccgacg ctggcggcgg
       61 gcgagagtgg cgcggcgagt cgaagcaagg ttgctgcctg cagtccccag acaagtcgcc
      121 agacgaaggt gtcggcggta acggcgagac cgacatgccc gctgatcact cgtgtggcca
      181 ttgcaaacag catgctgccg agaaagccga tcgtcagcgc gtgcagcggc atcaagccca
      241 gtctcggagc gcccgcaccc agcagtaccc aagcggcgtc gatggcggcc agcgccagag
      301 caagagggat ccagacaacc gccacgtgca gcatggccaa cagtcgcagt gacggtccgc
      361 gcaggctctg gaccagcccc cagcgaatgg ccagtgcaat gagtgcgaga ctgatgggcg
      421 cgaccacagt gagcgtcagc cacgagatgg atttagcgcc ggtgagatcg aagcgtccag
      481 cgacttgcag accgccgaaa accagaatgc ccgcgacaag cagccccagc acccagttcg
      541 gacgccacgg caccagggtg gtgaccgcat tcgaggtgaa gaacggcagt acgcggtgcg
      601 cgacggtcac ggtgatcgga gcgatgcacg cgaaaacgag cagccagacc gccgcggcca
      661 cgacgccgaa ttgatgcgtt gccaggcccg ctgcgaaaag cagggccgcc gcaatgccgg
      721 caatccagaa cgcgagcacg cagcgcgcat ggagccggtc acgtgcacgg ctcgaccgca
      781 cgagcgtcgc gaaggccacg ccgaccgcgg tccacgcgat cgcaagcagc agcgaaccga
      841 aggccgcggt acccggagaa acgacggtgc cgagaaggac cagcaagatg ccggcgacgt
      901 gcagccgtac cggccagatc agcaaggtcg cgggaggtga cggcacgtcg agccagcgcg
      961 gacccgccgt gaacatgaac cccgcgatga acagtggcat gtaggctgca gcgaagacaa
     1021 aaccgtgcgc aagtgtcggc ggcaatgccg tagtgaccga cacgcccgcc gaagacagga
     1081 agagccacac cgcccacgcg aacgacgcga gcagcaaact cacgccaccg gcaaagaacg
     1141 ccagccgatg cggcgacagc agcaagctgc gacttgcggc cttgggtgga gccgtcgtcg
     1201 ggcgcggacc tccttcgacc tgcgtcgccg gtgcaatggg aatggtcggg cgcgccatta
     1261 cggcagacat ccgtccgcag ttcggttccc ccgatgcctt gcagggcatc gagtgccgtc
     1321 gtcccgtcga agggaaggtg gcgtgcgtgg tgggcgtcgc agcatcgagg tgtcagggac
     1381 cgaaggccgg cacgccgctg gcatcgagtg acaaggccgg ctcctgcagt ttgtagggtc
     1441 cggtgccggc gttgtccgcc tgcgaggcgg gtacgaaacc gccgcgctgc acatcgaaga
     1501 cgtgcacttc accttcctcg atgacgtagt gccagccgtg cagcgtcaat gtgccggctt
     1561 cgacgcgctc tcgcaccatc ggatagtcca tcagccgttc gagctgcaga acgatggcac
     1621 gctgttcggt gcgtcgcagg acatcgggtc ccggttccgc gactggcagt acggcctcgc
     1681 gtccgagttc cagccagcgc acgaggttct gcgcggccgg tggcacgtct ccataaagcg
     1741 cgcggatcgc gccgcagtgg ctatggccgc agaccacaat gcgcttgaca tcgaggctga
     1801 gtaccgcgaa ctcgatcgcc gcggccgttc catggaatcc gtgtgactga tcgtagggcg
     1861 ggacgaatcc gccgacgttg cgcaccagga agagctcgcc gggcccggcg ccggtgagca
     1921 ggtacggcac gatccgagag tcgctgcagc cgatgaagag cgtcaacgga cgctggccgt
     1981 cgtcgacgag ctgctgaaac tgcgcgcgat actgcggaaa cgcatcgtcc ttgaagcgcc
     2041 gcagtcgctc gagcagttcg tcgggcaccg ccaggcccgt tcagcctatt ggaccagcgg
     2101 cgtgctggcg gcaaggaggt ctacgccttc gacccgggcg cgcccggcga gcagttgcag
     2161 atactggcgc aatgcggtcg cgtaggcctg gcgatcgagc gtcaggcgta ccgccgcctt
     2221 gacgtcctca taggtcggta ccgtgccggg ctcgcggccc agcacttcca ccacgtgcag
     2281 gccgaagcgg ctgcgaacca gtcgcggcag aactccgact tcgggttgac caaagatctc
     2341 tcgcgcgaac tcgggagcgc agtccgcagc agtcagccag ccgagctggc cgccgtcctg
     2401 tccactcggg cagttcgaca gttgcgccgc tgcctgcggg aatcgatcgg cctcgccgtg
     2461 cgtacgcgca cgcaggtcga tgaggcaggc ctcggcgcgc ttgcgcaggc cgtcgagatc
     2521 gactccgggt ggcacggcga acagcacgtg acgcagctga actttctcgc cgacggcgta
     2581 gcgggaagcg ttggcggcat ggaagcggcg gcaggtcggc tcgtcggcct cgggccgcac
     2641 gagctcgcgt tcgagcagcg cctcgattgc gtcggcggcc gcttcgctga tcgccccgcc
     2701 cgcaggggcc ggatcgtcag cgccgagcag gccggatgcg atggcggcct gacgcaggag
     2761 ttccacgttg gcgcggctcc gcagttcatt cggcgacagc acttcatcgg gggccgcgag
     2821 gtcgaggcca ttgacgcgcg gggccgtcgt cgactcaggg gtgcaggctg tcgcaagcat
     2881 cacgacaccc ggcccggaac gttctggccg gctggcacgt tcagccgccg cgagcgtaca
     2941 accaggtacg ggcggaacag gtaggcgatg gacgcaagcc cgctccacac gtgcaccagt
     3001 cggctgaacg gcagcagcag gaagatcgtc atgccgagca ccatgtggac cttgaacggc
     3061 caggcaatct cggtcagcag gttggcgtcg ggacggaacg tcgccacacc ctgcaggtag
     3121 gcggagagga ccagcatcgc gtgcgcgtcg acgcgatgcg cgtaggacag cggcagcgtg
     3181 atcagcccga gcagcagctg gatccacagg atgatcagga tcgcgagatc ggtgcggtgg
     3241 ctggtcagtc ggatacgagg atcaaacatc cggcggtgca gcagcatcgt cagcccgatg
     3301 aagcacatcg ttcccgcgat gccgccggag acaatggcga agagctgctt ggtcggagca
     3361 gtgatgaaag gctcgtagac ccagtgcggc gtcagcagac caacggtatg tccgaacagc
     3421 aggaacagga tgccgatgtg gaacaggttg ctgccccagc gcagctgttt cgcgcgcagc
     3481 atctgcgagg agtcgctctt ccacgtgtac tggtcgcggt cgaagcgggc caggctgccc
     3541 atgaagaaaa acgccaggca gacgtagggg tagacgttga agaagaagtt gtggagcagg
     3601 gtcatggcgg tctcctcagg cagccgtgcg atgcggcgct cgggcggagc ggacgatgtg
     3661 gacgggctgc gcttcgttcg ggcgcgactg gccctgggaa ctgcatccgc tgaaggcttc
     3721 gggctcggcc caactctcat cgatcgccgg ttcagtcgga agagcgaccc gttcggcgcg
     3781 ctcgcccgcc aggtccagca ccgcggccag cacgctggcg taggggctct ggcgctccag
     3841 caatgcgctg aagatcgcac gcacgacatg ggcaatttcg cgcaggaact cgcgcgcctg
     3901 cgtcggtggc tgcgtgctgg cgaactcgag gactgcaggc aggtagtcgg gcagctctcc
     3961 cggcgcaaga tgcaggccgg cacgctcgta ggtctgcgcc aggtcgacca tcgcgggtcc
     4021 gcggtcgcgg ctgtcgccgt gcacgtgctc gaacagatgc aatgccgtgc ggcggccgcg
     4081 gtcgaagatc tctacatact cagcttcggt gcgcaatgga gcggtgccga gaagctggga
     4141 gaccagcgcg tccagttcgc ccagccgacc ggagtcgagc gcggcctctg cgcgcagcgc
     4201 gtcgcgcagc tgtggcagca gcgcacgcag ctcggcgtca gggtagcgca gcagccaggc
     4261 cagtgcgcgc agcgtatgcg tcattttcgg tggggcagag tcgaaaggct tcatggtcac
     4321 accgtcgcct tgatggggat cgtgcgtttc ttttcgctgc cgaaaaggct cgtctcgctg
     4381 acgccttcgc tgcaaccgtt gccgaaggaa aatccgcagc cgccgcgcac gttgaacgcg
     4441 ttctcggcgt attcgcgatg cgttgtcggg atcacgaagc gatcctcgta attggcgatc
     4501 gccatcacgc ggtacatgtc ctcgacctcg gcacgcgtca ggccgacgcg ctcgagcacg
     4561 gcaggcttgt cgacgccgtc gacgtggcgg ctgcgcatga agacacgcat cgccatcatc
     4621 cgttcgagcg cgcgtgccac cggctcctcg tcgcccgcgg tcagaaggtt ggcgaggtac
     4681 ttcagcggga tgcgcaggct gcgcacgtcg ggcagttcgc cgttcatgcc gatgtcgccc
     4741 gcctgtgcac gcgaggtgac cggcgacagc ggcgggatgt accagaccat cggcagtgtg
     4801 cggtactcgg ggtgcagcgg caacgcgacg cgccagtcca ctgccatctt gtaaaccggt
     4861 gaggtcttcg ccgcagcaag ccaggcctcg ggaatgccgt cggcgcgcgc ctgctcgatc
     4921 ccgcgcggat cgttcggatc gaggaagatg ccgagctgcg ccgagtacag gttcctgtcg
     4981 tgctcggtgc tcgcggcctc ctcgatgcgg tcggcgtcat acagcagcac gccgaggtaa
     5041 cggatgcgac ccacacaggt ctcgctgcac acggtcggct ggcccgcctc gatgcgcggg
     5101 tagcagaaga tgcatttttc ggccttgccg ctcttccagt tgtagtagat ctttttgtag
     5161 ggacagcccg agacgcacat acgccagccg cggcacttgt cctggtcgat cagcacgatg
     5221 ccgtcttcct cacgcttgta gatcgacccc gacgggcaac tcgcgacgca cgccgggttc
     5281 aggcagtgct cgcacaatcg cggcaggtac atcatgaagg tgttctcgaa ctggccgtag
     5341 atctccttct gaatgtcgtc gaagttgcgg tccttgctgc gcttggaaaa ctcgccgccg
     5401 agtatttcct cccagttcgg gccccactcg atcttctcca tccgctggcc ggtgatcagc
     5461 gagcgcggcc gcgcggtcgg cgccgctttc atttc
//
LOCUS       DATIRT010000439         4066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1451380, whole genome shotgun sequence.
ACCESSION   DATIRT010000439 DATIRT010000000
VERSION     DATIRT010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451380"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VLN25_09900"
     CDS             <1..390
                     /locus_tag="VLN25_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HSQ10909.1"
                     /translation="SAVIADLVDVTRMLTADPENHVPHLAFQADAMATVPWLTIEDTI
                     TSYYFRMRVADQPGVLADITRILADGGISINALFQREPSEGENQTDIIMLTHDAVERE
                     VNAALRRIEALPTVLAPVIRIRQEELL"
     gene            402..1832
                     /gene="thrC"
                     /locus_tag="VLN25_09905"
     CDS             402..1832
                     /gene="thrC"
                     /locus_tag="VLN25_09905"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012213226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HSQ10910.1"
                     /translation="MRYVSTRGRAPERGFSDILLSGLADDGGLYVPSYYPQVTRADLD
                     AWRTLSYAELAAKIIGRYATDIALARLAQLCQATYAASVYSNTRPGSAAGDITPLRWL
                     EPGLALLELSNGPTLAFKDVAMQLLGALFEHVLAERGGRLNILGATSGDTGSAAEYAL
                     RGRPGINVFMLSPQGRMSEFQRAQMYSLQDVNIHNLAVSGTFDDCQDLVKAVAGDLEF
                     KAQWHIGSVNSINWGRIVAQVVYYFRGYLAATSRSDERVSFAVPSGNFGNILSGWIAR
                     RMGLPIDKLVLATNENDVLDEFFRTGRYRVRGGGETHATSSPSMDISKASNFERFMFD
                     IVGRDPGIVRELWWEIDTRGEFHLAGTPFFDVIRSCGVVSGRSNHVDRLRTIRLIEQR
                     HGVVIDPHTADGVKVALEHRDAPVPMICLETALPTKFAETIVEALGRSPERPAAFAGI
                     ETLPQRVETIGADPQVIKARIAAAGG"
     gene            1839..2333
                     /gene="mobB"
                     /locus_tag="VLN25_09910"
     CDS             1839..2333
                     /gene="mobB"
                     /locus_tag="VLN25_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HSQ10911.1"
                     /translation="MKAIGVIGYSNSGKTTLIERLIPIFRTRGLAVSAIKNAHHGFDM
                     DRPGKDSFRYREAGAGQVLIATALRWAMLTETPQQSATLEELLALLAPCDLVIIEGFK
                     SEGHIPRIEVRRASQAEPAIFPLDPNVVAVAADGPIDTGLPVLDLNAPAKIAEFISTH
                     LGLD"
     gene            2333..3523
                     /gene="glp"
                     /locus_tag="VLN25_09915"
     CDS             2333..3523
                     /gene="glp"
                     /locus_tag="VLN25_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HSQ10912.1"
                     /translation="MLTVEEALARMLKRADPVEVETVPTLEAHGRVLAADVISALSVP
                     PLDNSQMDGYAVRTLDVPAAGTTLRVSQRIAAGHVGVPLEPGTAARIFTGAPVPAGAD
                     AIVMQESCTADGDRVTLHETPQRGAWIRRAGIDIGTGDTVVAAGARLRPQDVGLIAAV
                     GAERVTVHRRLRVAVFFTGDELAMPGEPLSAGRIYNSNRFVLRALLERLGCDVVDLGI
                     IPDDRTRTRAAFVEAAARADLIITSGGVSVGEEDHVKPAVEAEGALELWQIAMKPGKP
                     VAFGRVRDVPFIGLPGNPVSSFVTFLLFGRPFILHRQGARSSAVQAFRLRADFDWPKP
                     DKRREFLRVRRNAENGVELFPTQNSAVLNSTAWANGLVDHPAGATIGRGDSVRYLSFS
                     DLLA"
     gene            3536..3793
                     /gene="moaD"
                     /locus_tag="VLN25_09920"
     CDS             3536..3793
                     /gene="moaD"
                     /locus_tag="VLN25_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HSQ10913.1"
                     /translation="MNVTVMYFAALRERIGCAREECELPSGVSTVGALRDWLVARGSP
                     WSEAFGEMQRVRTAVDQTMARDDTRIHDRAEVAFFPPVTGG"
     gene            3820..>4066
                     /locus_tag="VLN25_09925"
     CDS             3820..>4066
                     /locus_tag="VLN25_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HSQ10914.1"
                     /translation="MIRVQHEEFDVNAEIARLRVVNPRIGAIACFIGVVRDINEGAAV
                     ASMTLEHYPGMTEKALGDIVAQARARWELYDILVVHRV"
BASE COUNT          660 a         1302 c         1390 g          714 t
ORIGIN      
        1 tcggccgtga tcgccgatct cgtcgacgtg acgcgcatgc tgaccgcgga cccggaaaac
       61 catgtcccgc acctggcatt ccaggccgat gcgatggcaa cggtgccgtg gctcacgatc
      121 gaggatacga tcacctcgta ctacttccgc atgcgtgtcg cggaccagcc gggcgtgctg
      181 gcggacatca cgcgcatcct ggccgatggc ggcatttcga tcaatgcgct gttccagcgc
      241 gaaccgtcgg aaggcgagaa tcagaccgac atcatcatgc tgacgcacga tgccgtggag
      301 cgtgaagtca atgccgcgtt gcgccggatc gaggcgctgc cgaccgtgct cgcgccggtg
      361 atccgcatcc gccaggaaga gctgctgtag tggcggcggg catgcgctac gtctcgacgc
      421 gcggccgcgc tcccgagcgt ggattcagcg acatcctgct gtcggggctc gccgacgatg
      481 gcggcctcta tgttccctcg tactacccgc aggtgacccg cgctgatctg gatgcctggc
      541 gcacgctgtc gtacgcggag ctcgcagcta agatcatcgg tcggtatgcg accgacatcg
      601 cgctcgcgcg tctggcgcag ttgtgccagg ccacgtatgc ggcatcggtc tacagcaaca
      661 cccgtcccgg gagcgctgcg ggcgacatca cgcccctgcg ctggctggaa cccggcctgg
      721 cgctgctcga actgtccaac gggcccacgc tcgcgttcaa ggacgtggca atgcagctgc
      781 tgggtgcgct cttcgagcat gtgcttgcgg agcgtggcgg acggctgaac atcctcgggg
      841 cgacgtcggg cgacaccggc tcggcggcgg agtacgcgtt gcgcggacgg ccgggcatca
      901 atgtgttcat gctgtcgccg caggggcgca tgagcgagtt tcagcgggcg cagatgtact
      961 cgctgcagga cgtgaacatc cataacctgg cggtctcggg gacgttcgac gactgccagg
     1021 acctggtgaa agccgtcgcg ggagacctcg agttcaaggc gcaatggcac atcggcagcg
     1081 tcaactcgat caactggggt cgtatcgttg cccaggtcgt ctactacttc cgcggctacc
     1141 tcgcggcgac gagccgctcg gacgagcgcg tttcgttcgc agtgccatcc ggcaatttcg
     1201 gcaatattct ttccggctgg atcgctcgga ggatgggcct gccgatcgac aagctcgtgc
     1261 tggctacgaa cgagaacgac gtgctcgacg aattcttccg caccggtcgc tatcgggtgc
     1321 gtggcggggg cgagacccac gcgacgagca gcccgtcgat ggacatcagc aaggcctcca
     1381 acttcgagcg cttcatgttc gacatcgtcg ggcgcgatcc gggcatcgtg cgcgaactgt
     1441 ggtgggagat cgacacacgc ggcgagttcc acctggcggg aacgccattt ttcgatgtca
     1501 tccgctcgtg cggcgtggtg tcgggacgca gcaaccatgt tgatcgcctg cgcacgatcc
     1561 ggctgatcga gcagcgccac ggtgtcgtga tcgatccgca caccgcggac ggcgtcaaag
     1621 tcgcactgga acatcgcgac gcgcccgtgc cgatgatctg cctcgaaacg gcgctgccga
     1681 ccaagttcgc cgaaacaatc gttgaagcgc tcggacgttc gccggagcgg ccggcggcat
     1741 ttgccggcat cgaaaccctg ccgcagcggg tcgaaacgat cggcgcggat ccgcaggtca
     1801 tcaaggcccg tatcgcagct gcgggcggtt aagcgcgcat gaaggcgatt ggcgtcatcg
     1861 gctattcgaa cagcggcaag accacgctca tcgagaggct gattccgatc ttccgcacgc
     1921 gcggcctggc ggtgtcggcg atcaagaacg cgcaccatgg tttcgacatg gaccgcccgg
     1981 gcaaggactc gtttcggtac cgggaggcgg gtgcgggaca ggtcctgatt gcgaccgcgc
     2041 tgcgctgggc catgctgacc gagacaccgc agcaatcggc aactctcgag gaactgctgg
     2101 cgttgctggc cccgtgcgac ctggtcatca tcgaaggctt caagagcgag ggtcacatcc
     2161 cgcggatcga agtgcgccgc gccagccagg cggaaccggc catctttccg ctcgacccga
     2221 acgtcgtggc ggtggccgcc gacggcccga tcgacaccgg gcttccggtg ctggacctga
     2281 acgcgcccgc taagattgcc gagttcatat cgacccatct ggggctcgac tgatgcttac
     2341 agtggaagag gcgctcgcgc gcatgctgaa gcgtgcggat ccggtcgaag tcgagaccgt
     2401 gccgacgctg gaggcgcatg gccgcgtcct tgcggccgat gtcatctcgg cgctctcggt
     2461 gcctccgctc gacaactccc agatggacgg ctacgcggtg cgcacactcg atgtgccggc
     2521 ggcggggacg accttgcgcg tgagccagcg gatcgcggct ggtcacgtcg gcgtgcctct
     2581 cgagccgggc acggctgccc gcatcttcac cggggcgcct gttccggcag gtgccgatgc
     2641 catcgtcatg caggagtcct gtaccgcgga cggcgatcgc gtcacgctcc atgagacgcc
     2701 gcagcgtggc gcatggatac ggcgcgcggg catcgacatc ggcaccggtg atacggtggt
     2761 cgccgcggga gcgcgactgc gtccccagga tgtcggtctg atcgcggccg tcggcgccga
     2821 gcgggtcacg gtgcaccggc gactgcgcgt cgccgtgttc ttcaccgggg acgaactggc
     2881 gatgcctggt gagccgctgt cggccggccg catctacaac tccaatcgat tcgtgctgcg
     2941 cgcgttgctg gaacgtctcg ggtgcgacgt ggtcgacctc ggcatcattc cggacgatcg
     3001 cacacgcacc cgggccgcgt ttgtcgaggc cgctgcgcgc gccgatttga tcatcacgtc
     3061 gggcggggtg tcggtcggcg aagaagatca tgtgaagccg gcggtcgaag cggagggcgc
     3121 gctcgagctg tggcagatcg cgatgaaacc gggcaagcca gtggccttcg gtcgggtgcg
     3181 cgacgtccct ttcatcgggc tgccgggcaa tccggtctct agcttcgtga cgttcctgct
     3241 cttcggccgt cccttcatcc tccaccgaca gggcgcgcgt tcgagtgcag tccaggcctt
     3301 ccgcctgcgc gcggatttcg actggccgaa gcccgacaag cgacgcgagt tcctgcgcgt
     3361 gcgacgcaac gcagagaacg gagtggaact gtttccgacg cagaattcgg cggtgctgaa
     3421 ctcgacggcg tgggccaatg gtctcgtcga ccacccggcg ggcgcaacga taggccgtgg
     3481 tgactccgtc cgttacctgt cgttttccga tctacttgcc tgaggtgcgg tctcgatgaa
     3541 cgtcacggtc atgtacttcg ctgcgctgcg tgagcgcatc ggttgtgcgc gcgaggagtg
     3601 cgagttgccg tccggcgtga gtaccgtggg cgcgttgcgc gactggctcg tggcgcgcgg
     3661 ctcgccctgg tccgaagcct tcggcgagat gcagcgtgtg cgcacggccg tcgaccagac
     3721 gatggcgcgc gacgacaccc ggatccacga tcgtgccgaa gtcgcgttct tcccgccggt
     3781 gacgggcggc tgagctggcc ggacgatgga ggcccgacga tgatccgcgt gcagcacgag
     3841 gagttcgacg taaacgcgga gatcgcgcga ctgcgggtgg tcaacccgcg catcggcgcg
     3901 atcgcttgct tcatcggcgt cgtgcgcgac atcaacgagg gcgccgcggt cgcatcgatg
     3961 acactcgaac actacccggg catgactgag aaagcgctcg gtgacattgt cgcgcaggcg
     4021 cgcgcgcgct gggaactcta cgacatcctg gtcgtccatc gtgtcg
//
LOCUS       DATIRT010000440         3449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_763545, whole genome shotgun sequence.
ACCESSION   DATIRT010000440 DATIRT010000000
VERSION     DATIRT010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763545"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..160)
                     /locus_tag="VLN25_09930"
     CDS             complement(2..160)
                     /locus_tag="VLN25_09930"
                     /inference="COORDINATES: protein motif:HMM:NF017847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HSQ10915.1"
                     /translation="MGDEIIRAQTIAYDDFVAYKGEQGAKEAGKMRAEGKEYVVRDGD
                     VLNFLFNV"
     gene            143..262
                     /locus_tag="VLN25_09935"
                     /pseudo
     CDS             143..262
                     /locus_tag="VLN25_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477050.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
     gene            269..1105
                     /locus_tag="VLN25_09940"
     CDS             269..1105
                     /locus_tag="VLN25_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HSQ10916.1"
                     /translation="MTASIDQLFIYPIKGCRGIAVQHATLTATGLEIDGIGDRERVVV
                     DEHGEFLSQRELPAMALIETRLTSTTLRLKAPGMLQLEVPFASEGDVLNVTVWNDTVA
                     AVTQGEVADAWFSRYLGQTCRLMRFDPEARRLSNVRYTGATQAPYKFADAFALLIAST
                     ASLADLNARLGKRGIAAVGIERFRPNLVLAGIEAFEEDYLERVTIGTTELRVVKPCVR
                     CSVPNVDPATGETSTEPGDTLALYRDNARAGGVTFGVNAIVIQGDAELRPGDAAELTL
                     AL"
     gene            complement(1183..2349)
                     /locus_tag="VLN25_09945"
     CDS             complement(1183..2349)
                     /locus_tag="VLN25_09945"
                     /inference="COORDINATES: protein motif:HMM:NF016150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HSQ10917.1"
                     /translation="MTGVDPRDARLDALRGFALFGILLVNIQSYLSGSTNAIGYLTAE
                     AGPADRVAFFLTAALVAGKFMPLFGMLFGASFALLYDKLRATVVDPRGIYRRRLVFLL
                     IFGVLHGLFLYFGDITHAYAIAGFVLLRHADADADSVARATVRWWIVAVAWLLLLIAA
                     SSGEPEVASIESLEEVYLNVAASSRLGYWEQWPLRARMFLWQVQSNLLGLPTVIALMM
                     TGMLAQRAGWLRDPHVPAWRPAARLGLWLGLPAGLVYGGWSVTHAEIENGLALPAVQI
                     LVLTVSLSLSFFYVATFLRRASPLVITWLAPAGRMALTNYLAQSVAMGLLLSGWGFGI
                     DTLAYWQLSAVAIAIFMLQLVASRWWLGRHAQGPLEAMWRAWTYRGALRPSAAG"
     gene            complement(2366..2863)
                     /gene="coaD"
                     /locus_tag="VLN25_09950"
     CDS             complement(2366..2863)
                     /gene="coaD"
                     /locus_tag="VLN25_09950"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006760399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HSQ10918.1"
                     /translation="MVIAVYPGTFDPLTRGHEDLVRRAAGLFSKLVVGVADSRNKKPF
                     FVLDERIAIAREVLSHYPNVEVSGFRGLLKDFVRQQNARVIVRGLRAVSDFEYEFQMA
                     GMNRYLLPDVETMFLTPSDQYQFISGTIVREIAQLGGDVSKFVFPSVEKWLKAKIEQM
                     GGSTE"
     gene            complement(2910..>3449)
                     /locus_tag="VLN25_09955"
     CDS             complement(2910..>3449)
                     /locus_tag="VLN25_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HSQ10919.1"
                     /translation="GSWKRTLLPVPDIPGLRPTPDRVRVTAFNWLLHLRPDATALHGL
                     DLFAGTGALGFELASRGARRVTLVEMHPRALEGLRATRTRLDARQVEIIAGDALSVAA
                     SLPAGSFDVVFLDPPFDSGQQAPALSAARRLLASDGLIYLEAPAPLPPEQAQQAGLEI
                     VRQGNAGQVAFHLLRVPTA"
BASE COUNT          653 a         1148 c         1108 g          540 t
ORIGIN      
        1 gtcagacatt gaacaggaag ttcagtacat caccgtcgcg cacaacgtac tccttgccct
       61 cggcgcgcat cttgccggct tccttggcac cctgttcgcc cttgtaggcg acgaaatcat
      121 cgtaggcgat ggtctgcgcg cggatgattt cgtcgcccac aagggcgaac agggtgccaa
      181 ggaagccggc aagatgcgcg ccgagggcaa ggagtacgtt gtgcgcgacg gtgatgtact
      241 gaacttcctg ttcaatgtct gacgcgctat gactgcctcc attgatcagt tgttcatcta
      301 tccgatcaag ggctgccgcg gcatcgccgt ccagcacgcg acgctgacgg cgaccggact
      361 tgagatcgat ggcatcggtg accgcgagag ggtcgtggtc gacgagcacg gcgaattcct
      421 gagtcagcgc gagctgccgg ccatggcact gatcgagacg cgcctgacgt cgacgacgct
      481 gcggctcaaa gcgcccggca tgctgcagct cgaagtcccg ttcgccagcg aaggcgacgt
      541 gctcaacgtc acggtctgga acgacaccgt ggccgccgtc acgcaaggcg aagtcgccga
      601 tgcgtggttc tcgcgctatc tcggccagac gtgccgcctg atgcgcttcg atcccgaagc
      661 gcggcggctg tcgaacgtgc gctacaccgg agcgacgcag gcaccctaca agttcgccga
      721 tgccttcgcg ctgctcattg cgtcgaccgc ctcgctggcc gacctcaatg cgcggttggg
      781 caagcgcggc atcgctgcgg tcggcatcga acgtttccgc ccgaacctcg tgctggccgg
      841 aatcgaggcc ttcgaggagg actatctcga gcgcgtcacg atcggcacca cggaacttcg
      901 cgtggtcaag ccctgcgtgc gctgttcggt gccgaacgtc gatccggcga cgggtgaaac
      961 atccaccgag cccggcgata cgctggccct ctatcgggac aatgcgcggg cgggcggtgt
     1021 gactttcggc gtgaacgcca tcgtgatcca aggtgacgcc gagctgcggc caggtgatgc
     1081 cgctgaactg acgctcgcgc tctgaaccca aggatcaacg gtcgcacagc gacgcctttt
     1141 gcgaggtggt cgtgtcgcgc tgagtttccg ggatccaatt cgtcagccgg cggctgacgg
     1201 gcgcagggct cctcgatagg tccaggcgcg ccacatcgct tcaagtggac cctgcgcatg
     1261 gcggcccagc caccagcggc tggcgacgag ctgcagcatg aaaattgcga tggccacggc
     1321 gctgagttgc cagtaggcca gcgtatcgat tccaaatccc cagccggaca acagcaggcc
     1381 catggccacg gactgcgcca ggtagttcgt cagtgccatg cgtccggctg gcgccagcca
     1441 ggtgatgacc aaaggcgatg cacgccgcag gaacgtcgcg acataaaaaa acgagagcga
     1501 caggctcact gtcaacacga ggatctgcac cgccggcagg gccaggccgt tctcgatctc
     1561 tgcgtgcgtg accgaccacc cgccatagac gagtccggcg ggcagcccca accacaaacc
     1621 gagtcgtgcc gccggtctcc acgccggcac gtgcgggtcc cgcagccagc cggcgcgctg
     1681 ggccagcatg ccggtcatca tcagggcaat gaccgtgggc aggcccagaa ggttggactg
     1741 gacttgccat agaaacatac gcgcccggag cggccactgc tcccagtagc cgagccggct
     1801 gctcgccgcg acgttcaggt agacctcttc cagactctcg atcgacgcga cctcgggctc
     1861 gccactcgac gcggcgatca gcagcagcag ccaggcgacg gcaacgatcc accagcgtac
     1921 ggtcgctctc gccacgctgt ccgcgtctgc gtctgcgtgc cggagcagca cgaatccggc
     1981 gatggcatag gcatgcgtga tgtcaccgaa gtagaggaac agtccgtgca gcacaccgaa
     2041 aatcagcagg aacaccagtc gccggcgata gatcccgcgc ggatcgacaa cggtggcccg
     2101 cagcttgtcg tagagcagcg caaagctggc cccgaacagc atgccgaaca gcggcatgaa
     2161 cttgccggcc accagagcag cggtcaggaa gaatgcaacc cggtcggccg gaccggcctc
     2221 ggctgtcagg tatccgatcg cgttggtcga tccggacaga tacgactgaa tgttgacgag
     2281 caggatgccg aacagtgcga aaccgcgcag ggcgtcgagg cgcgcgtcgc gcgggtcgac
     2341 gccggtcacg ccgcgcttcg gaacatcatt cggtcgatcc gcccatctgc tcgatcttcg
     2401 ccttcagcca cttctccacc gacgggaaaa cgaacttgct gacgtcgccg ccgagctgcg
     2461 cgatctcgcg cacgatcgtg cccgagatga actggtactg gtccgaaggc gtgaggaaca
     2521 tcgtctcgac gtcgggcagc agatagcggt tcatcccggc catctggaac tcgtactcga
     2581 agtccgacac agctcgcagg ccgcgcacga tcacgcgcgc gttctgctgg cgcacaaagt
     2641 ctttcagcag cccgcgaaag cccgacacct cgacgttcgg gtagtgcgac agcacctcgc
     2701 gcgcgatcgc gatccgctca tcgagcacga aaaacggctt cttgttgcga ctgtcggcca
     2761 caccgacgac gagcttggaa aacagtcccg ccgcacgccg gaccaagtcc tcgtgaccgc
     2821 gagtcagcgg gtcgaacgta ccgggataga cggcgatgac catggtgcct cctcagcgtc
     2881 gcacggggcg gttgggcgcg agggattatt catgcagtcg gcacccgcag caaatggaac
     2941 gccacctggc cggcgtttcc ctggcgaacg atctcgagtc cggcctgctg cgcctgctcc
     3001 ggcggcaacg gtgcgggcgc ctcgaggtag atcaggccgt cgcttgccag caggcggcgc
     3061 gccgcgctca gcgccggcgc ctgctggccg gagtcgaagg gcggatcgag gaagaccacg
     3121 tcgaacgaac ctgccggcag gctcgctgcc accgagagcg catcccccgc gatgatctcg
     3181 acctgcctgg catcgaggcg cgtccgcgtt gcgcgcagac cttcgagcgc gcgcgggtgc
     3241 atttcgacca acgtcacacg ccgcgcaccc cgcgacgcca gttcgaatcc gagcgcgccg
     3301 gtgccggcaa aaaggtcgag gccgtgaagc gcggtggcat ccggccgcag gtgcaacagc
     3361 cagttgaatg ccgtcacgcg cacccggtcc ggcgtcggcc gcaggcctgg gatgtcgggc
     3421 accggaagca acgttcgctt ccacgaccc
//
LOCUS       DATIRT010000441         3575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_172263, whole genome shotgun sequence.
ACCESSION   DATIRT010000441 DATIRT010000000
VERSION     DATIRT010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172263"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..1803
                     /locus_tag="VLN25_09960"
     CDS             208..1803
                     /locus_tag="VLN25_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSQ10920.1"
                     /translation="MAASSLDVLIIGAGHNGLVCASYLAAAGLRVRMVERRGLVGGAA
                     VTEEFHPGFRNSTASYTVSLLNPTVIRDLRLRERGLRIIERPYSNFVPLADGRSLKFG
                     GDLAATQAEVAKFSTRDAERLPAFYARLDRLAGLLKQWVLQAPPNLGGGMVVEGWRDL
                     IAAAKLLLQFGQLPREGQRDLVDVFTKSAGDWLDHWFESEPLKAVLGWDSVVGNYASP
                     YAAGSAYVLLHHCFGEVNGQAGKWGHAIGGMGAITQAMAAEARERGVEITLDAPVQRV
                     IVEHGAAVGVELTGGEVVRARATASNVNPKLLYQRLLQPSELPVEFVESIGRYKCGSG
                     SFRINVALSELPDFVAAPGSNAQPHHASGIVFAPSLAYMDQAWRDAVADGCSTAPVVE
                     MLIPSVVDDTLAPAGAHVASLFCQQFDPEADWSRLKPIAVERIFDVVQAHCPNFRTSL
                     LGCRALSPQDLEDEFGLVGGDIFHGQLSLDQLFSMRPIMGYARYRGPLKGLYHCGSGA
                     HPGGGVTGAPGHNAAREIIRDLT"
     gene            1860..2306
                     /locus_tag="VLN25_09965"
     CDS             1860..2306
                     /locus_tag="VLN25_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HSQ10921.1"
                     /translation="MIDTAGMFDFSAETGFVALFFASFVAATVLPGGSEFVLIAVIHQ
                     HPDAIWQAVLVATVGNTLGGLTSYWVGRLIPNRVHHKSIIYLHKYGYWTLLFSWVPLA
                     GDALAVAAGWLRFNPWISLAAFAVGKFFRYVLVTGGWAWFEALAWP"
     gene            2422..3171
                     /locus_tag="VLN25_09970"
     CDS             2422..3171
                     /locus_tag="VLN25_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HSQ10922.1"
                     /translation="MKILVTVKRVVDYNVKVRVKSDGSGVDIANVKMSMNPFDEIAVE
                     EAVRLKEAGVATEIVAVSCGVPQCQETLRTAMAIGADRGILVETDVELQPLAVAKLLK
                     ALADKEKPNLIIVGKQAIDDDANQTGQMLAALLDIPQGAFASKLKIADGRADVTREVD
                     GGLETISISLPAVVTADLRLNEPRYVTLPNIMKAKKKPLETIKPADLGVDVTPRLKTL
                     KVSEPPARKAGIKVPDVATLVSKLKNEAKAI"
     gene            3189..>3575
                     /locus_tag="VLN25_09975"
     CDS             3189..>3575
                     /locus_tag="VLN25_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HSQ10923.1"
                     /translation="MTALVVAEHDNASIKGPTLNAVTAAAKAAGDVHVLIVGHNAARA
                     AQAAAKVGGVSKVLVADGPQFADGLAEPVASQVLAVAGNYSHVLAPATAYGKNFMPRV
                     AAKLDSAQISEISSVESADTFTRPIYA"
BASE COUNT          607 a         1150 c         1195 g          623 t
ORIGIN      
        1 agaaccagat cgccgcgcag cgcatcctcg atcgcgtcgg ctaccgctga acgagcgcag
       61 accggtcatg tacatcggca tctgtaatgc gataacggat tgacagatcc gctaggcggt
      121 cgcggcccgg cgggctgagc cgctcgcgcc gcccgcggga ccggcgccga cccgactgga
      181 cggtacggtc gctaagatcg cgccggcatg gctgcatcct ccctcgacgt cctgatcatc
      241 ggcgctggcc acaacggcct cgtttgtgcc agctatctcg cggctgcggg cctcagagtg
      301 cggatggtcg aacgccgcgg tcttgtcggc ggagccgcgg tgaccgagga attccacccg
      361 ggctttcgca attcaacggc cagctacacg gtgagcctgc ttaacccgac ggtgattcgt
      421 gatctgcgcc tgcgcgagcg cggcctgcgc atcatcgagc gtccgtactc caacttcgtg
      481 ccgctcgccg atggccgatc gctgaagttc ggcggcgatc tcgcggccac ccaggcggag
      541 gtcgcgaagt tctccacgcg ggacgctgaa cgactgcccg ctttctatgc gcggcttgat
      601 cggctcgccg gcctgctcaa gcagtgggtg ctgcaggcac cgccgaatct cgggggaggg
      661 atggtggtcg agggttggcg cgacctgatc gcggcggcga agctgctgtt gcagtttggg
      721 caattgccac gcgaaggcca gcgcgacctc gtcgatgttt tcaccaagag cgcgggggac
      781 tggctcgacc attggttcga gtccgagccg ctgaaggcgg tactgggctg ggactcggtc
      841 gtgggcaact acgccagccc ctacgcggcg ggctctgcct acgtgctgct gcatcactgt
      901 ttcggcgagg tcaacggcca ggcgggcaaa tggggtcatg cgatcggtgg catgggtgcg
      961 atcacgcagg cgatggcggc cgaagcacgc gaacgcggcg tggaaatcac gctggacgcc
     1021 ccggtgcagc gagtcatcgt cgagcacggc gctgcggtcg gcgtcgagct gaccggtggc
     1081 gaagtcgttc gcgcgcgcgc cactgcatcg aacgtcaacc ccaagctgct gtatcaacgg
     1141 ctgctgcagc cgtcggagtt gccggtcgaa ttcgtcgagt ccatcgggcg ctacaagtgc
     1201 ggctcgggtt cgtttcgcat caacgtcgcg ctgtcggaat tgcccgactt tgtcgccgcg
     1261 cccggctcga acgcgcaacc gcaccacgcg agcggcatcg tgttcgcacc ttcgcttgcg
     1321 tacatggacc aggcttggcg cgatgccgtg gccgacggct gctcgaccgc gccggtcgtc
     1381 gaaatgctga tccccagcgt cgtcgacgat acgctggcgc cggcgggcgc ccacgtcgcg
     1441 agcctgttct gccagcagtt cgatcccgag gcggactggt cgaggctgaa gccaatcgcg
     1501 gtggaacgca tcttcgacgt cgtgcaggcg cattgcccga atttccgcac atcgctgctc
     1561 ggatgtcgcg cattgtcgcc acaggacctg gaggacgagt tcgggctggt cggcggtgac
     1621 atcttccacg gacagttgtc gctcgaccag ttgttctcga tgcggccaat catggggtac
     1681 gcgcgctacc gcggtccgct gaagggcctg tatcactgcg gctccggtgc gcatccggga
     1741 ggcggcgtga ctggcgcgcc cgggcacaat gctgcacgcg agatcatccg cgatctgacg
     1801 tagacggccg acgtcggcgc gccggtcccg tctagactcg cgcgctttgc gccccgcgcg
     1861 tgatcgacac cgcaggcatg ttcgacttct ccgcagaaac cgggtttgtc gctctcttct
     1921 tcgcgagctt cgtcgccgcc acggtgctgc ccggtggctc cgagttcgtg ctgatcgcgg
     1981 tgatccacca gcatccggac gccatctggc aggcagtcct ggtggccacg gtcggcaaca
     2041 cactgggcgg gctgacgtcg tactgggtcg ggcggctgat acccaaccgg gtgcatcaca
     2101 agtcgatcat ttatctgcac aagtacggct actggacgct gctgttctca tgggttccgc
     2161 tcgccggcga tgcgctcgcc gtcgccgcgg ggtggttacg attcaaccca tggatctcgc
     2221 tggcggcctt cgcggtcggc aagttctttc gctacgtcct ggtcaccggc ggctgggcgt
     2281 ggttcgaggc gctggcgtgg ccctgagtcc cccctctacg gagggggccg gacggcctgg
     2341 cggcgactcc tataattctc cggtttctcc gatcgagtcc gcagcgccca tgcgtggcgc
     2401 attccttacc taagcgaacc catgaaaatc ctggtcacgg tcaagcgtgt ggtcgactac
     2461 aacgtcaagg tccgggtcaa gtcggacggc agcggagtcg acattgccaa cgtcaagatg
     2521 tcgatgaacc ccttcgacga gattgccgtc gaggaagcgg tgcgcctgaa ggaggccggc
     2581 gtggcgacgg agatcgtggc cgtgtcgtgc ggcgttccgc agtgccagga gacgttgcgc
     2641 accgcgatgg cgatcggcgc cgaccgtggc atcctggtcg agaccgacgt cgaactgcaa
     2701 ccgctggcgg tcgccaagct gctcaaggca ctggccgaca aggaaaagcc caatctcatc
     2761 atcgtcggca agcaggccat tgacgacgac gccaaccaga ccgggcagat gctcgcggca
     2821 ctgctcgaca tcccgcaggg cgccttcgcg tccaagctca agattgccga cggccgtgcc
     2881 gacgtgacgc gcgaagtcga cggcgggctt gagacgatct cgatcagtct gccggcggtc
     2941 gtgaccgccg acctgcgcct taacgagccg cgctacgtca cgctgccgaa catcatgaag
     3001 gcgaagaaga agccgctcga gacgatcaag ccggccgatc tcggcgtcga cgtgactcca
     3061 cgattgaaga cactcaaggt gagcgagccg ccggcgcgca aggcgggcat caaggtgccg
     3121 gacgtcgcga cgctcgtaag caaactgaag aacgaagcta aagccatctg aaagtcgaga
     3181 gacacgacat gactgcactt gtagttgcgg aacacgacaa tgcgtcgatc aagggcccga
     3241 cactcaacgc ggtgaccgcg gccgcgaagg ctgcgggcga cgtgcacgtg ctgattgtcg
     3301 gccacaacgc ggcccgtgcg gcgcaggccg cggcgaaagt cggtggtgtc agcaaggtgc
     3361 tcgtggccga tggcccgcag ttcgccgacg gcctggccga accggttgcg tcgcaggtgc
     3421 tggcggtcgc agggaattac tcgcatgtcc tggcaccggc cacggcctac ggcaagaact
     3481 tcatgccgcg cgtcgcggcg aagctcgact cggcacagat atccgagatc agcagcgtcg
     3541 aatcggccga cacgttcaca cgcccgatct acgct
//
LOCUS       DATIRT010000442        12017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_861867, whole genome shotgun sequence.
ACCESSION   DATIRT010000442 DATIRT010000000
VERSION     DATIRT010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12017
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861867"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1559)
                     /gene="ilvD"
                     /locus_tag="VLN25_09980"
     CDS             complement(<1..1559)
                     /gene="ilvD"
                     /locus_tag="VLN25_09980"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HSQ10924.1"
                     /translation="MPHNDRSKHVTQGVARSANRAMYYAMGYKDEDFDKPMIGVANGH
                     STITPCNSGLQKLADAACEEIAACKANSQVFGTPTISDGIGMGTEGMKYSLVSREVIA
                     DCVETCVNGQWMDGVIVIGGCDKNMPGGMMGIARCNVPAIYVYGGTVKPGRYKDKDLT
                     IVSAFEAVGEFTAGRLNETDFKEIERRCIPGSGSCGGMYTANTMSSSFEALGMSLLYS
                     STQANPDEDKVTSTREAARVLIAAVKNNLRPSDIITRESIENAVSLIMAVGGSTNAVL
                     HYLAIAHSAGIPWTIDDFERIRRKVPVLCDLKPSGKYVATDLHSAGGVPQVLKVLLAN
                     GLLHGECMTITGKTMAAMLADVPAEPAASQDVIHPFSRPMYAQGHLAILKGNLSPEGC
                     VAKITGLKNPVITGPARVFDSEDAAMEAILADRIRPGDVLVIRYEGPKGGPGMREMLS
                     PTSALIGKGLGDSVGLITDGRLSGGTWGMVVGHVAPEAYVGGTIALIEEGDSVTIDAH
                     RLLLELNVADT"
     gene            1648..2466
                     /gene="lgt"
                     /locus_tag="VLN25_09985"
     CDS             1648..2466
                     /gene="lgt"
                     /locus_tag="VLN25_09985"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HSQ10925.1"
                     /translation="MPPIHHFDPIALQIGPLAIRWYGLMYLIGFALFLVLGKLRSRET
                     WRGMSSQDVDDLLFYGMIGVIVGGRLGHVIFYGPLTYYLSHPLEIFAVWKGGMASHGG
                     ILGVIAAMAWFARSRGKSFFGVADFVVPLVPLGLAAGRIGNFINGELWGRVADPANVP
                     WAMIFPQAGDNLPRHPSQLYQFALEGLLLFAILWVYSRRPRPVGTAGSLFLIGYGVLR
                     IVGEFFREPEALYGAMPLGLSAGQWLSVPMVLIGIAFFVVFHRRGLSPVAAAKR"
     gene            2581..3534
                     /locus_tag="VLN25_09990"
     CDS             2581..3534
                     /locus_tag="VLN25_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HSQ10926.1"
                     /translation="MIAVLAAGVLAAVPASAQSWPNKPITLIVPFPPGGGTDAFARPL
                     SAQLTKQLGRQVIIDNRGGAGGTVGASAAAKAAPDGYTFFVGAVHHAIAPSFYPKLDY
                     NIETDLVPLTVIANPPQIVVINPSRVKVEDFKAFMTLLRANPGKLNFASAGNGTSHHL
                     AGELFKQQSNTFIVHIPYRGAGPALQDLITGNVDMMFDGLGSSAQHIRSGRIKALAVA
                     APKRSPAFPNVPTTAEVGMPDYVVSTWYGVWGVKGTPKEILDRMHAEIVKAVGSPELK
                     DIWAGNGSDTATMSQQDFAKFLNSEIKRWAAVTKSSGAKLD"
     gene            3543..4325
                     /locus_tag="VLN25_09995"
     CDS             3543..4325
                     /locus_tag="VLN25_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HSQ10927.1"
                     /translation="MSGVVHLRSAGETATVVLESPGKLNAISESMWHALARVFSTFAE
                     APGLRAVVIRGAGHNFAAGADIEEFERVRGQPADGRRYHLETIANALAAIGACPVPVI
                     AAIEGVCVGGGLEVAIASDLRIAADNARIGAPVGRLGFPFALPELVPLLRLVGPGVAA
                     ELLLEGRLFEAHEAAARGLVTRAVALDRFDAELRECVSRIVAGSPLSARENKANIRRL
                     TATGGVYTERELDASFAFLQSEDYREGVAAFRAKRPARFTGK"
     gene            4357..5937
                     /locus_tag="VLN25_10000"
     CDS             4357..5937
                     /locus_tag="VLN25_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA synthase"
                     /protein_id="HSQ10928.1"
                     /translation="MTSDSSFYSLIERRFPADLDSACIETADGLLYSWNDLHHASGRL
                     AHWLDSLRLPAGARVAVQVEKSPECLMLYLATLRAGLVYLPLNTAYQRAEIEYFLADA
                     EPDVLVCAPARQAELEPLARRARCHHVVTLGEAREGSLLEAAAPFGDVYTTRARAAAD
                     LAAILYTSGTTGRSKGAMLTHGNLASNALTLDEFWGFAAEREAGRQDVLLHALPLFHV
                     HGLFVASHAALLAGAKTLMLSKFEVAEVLERLPRSTVMMGVPTYYTRLLAEPTFDMAR
                     CKSVRLFVSGSAPLLAETHREFEARTGHRILERYGMSETVMLVSNPYFKERGSRIAGT
                     VGQPLPGVGVRVVGEDGSVCPTGDIGSVEVRGPNVFAGYWRMPEKTAEEFTADGWFCT
                     GDVGHFGGRGVPDHYLTLVGRSKDLIISGGFNVYPKEIEGYIDEMPGVVESAVIGLPH
                     PDFGEAVVAVIVARRPGAFDARSVVDGLKGRIANYKVPKRVFVVEDLPRNTMGKVQKN
                     VLRDRYKATFTPQTRSAA"
     gene            complement(5909..6469)
                     /locus_tag="VLN25_10005"
     CDS             complement(5909..6469)
                     /locus_tag="VLN25_10005"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HSQ10929.1"
                     /translation="MTDQIVVTPYDPAWPARFRIESQLIQVALSDLRPAIEHIGSTSV
                     PGLAAKPIIDMLVGVRSLDEFERHYDRLSIYGYEYIPEYERVLPDRRFFKRVVRDVRT
                     HHVHVVELNGLYWKRYVKFRDNLRADAWLAARYAELKRRLASRFRFDRDAYTNGKSGF
                     VEAVLAMPGRSSPRGTSLRPHFAFAA"
     gene            complement(6603..6821)
                     /locus_tag="VLN25_10010"
     CDS             complement(6603..6821)
                     /locus_tag="VLN25_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10930.1"
                     /translation="MHDPTALPTPAVVRKPRPLVRRRESRLPVVLVLLAGCVAGAAFV
                     ALIEAPLPEEIVLASLERAMGPAARLAR"
     gene            complement(6995..7636)
                     /locus_tag="VLN25_10015"
     CDS             complement(6995..7636)
                     /locus_tag="VLN25_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HSQ10931.1"
                     /translation="MTASEQAISEIGDPMVRELATRGQVRTFPKNAVIINEGDRGDSL
                     YVILTGKVKVYVSDDDGREMILDIYGPGDYVGEMALDGRPRAASVMTLEPTSCSVVTR
                     DELRSAIGANPDIAMALITTLIDRARIATDNVKNLALMDVYGRVARLLLSLAKEDGGK
                     LVVPERMTQQDIADRVGASRDMISRIFKDLTIGGYVTIENRTITIHRKPPARW"
     gene            complement(7732..8541)
                     /locus_tag="VLN25_10020"
     CDS             complement(7732..8541)
                     /locus_tag="VLN25_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HSQ10932.1"
                     /translation="MEVGFIAALVLLGAGTGFAAGLLGIGGGMLMVPFLTLLFTWRGF
                     PLEHIVHMAIATSLATILFTSVSSVRAHHQRGAVLWPVVRLLAPGILLGSLIGAQIAG
                     RLPTFWLSVVFATFVGFSALQMLLDRKPRPTRELPKGAGMFGVGSGIGFISSLVGAGG
                     GFISVPFMVWSNVKIHNAVATSAALGFPIAAAGTLGYILAGHGEASLPPGTAGFVYLP
                     ALLAIAAASVLTAPLGARVAHSLDTKPLKRVFAILLFLLAGYMAYKGARAY"
     gene            complement(8541..9011)
                     /locus_tag="VLN25_10025"
     CDS             complement(8541..9011)
                     /locus_tag="VLN25_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HSQ10933.1"
                     /translation="MSYWLMKSEPDECSIDDALAAPKKTVPWTGVRNYQARNFMRDGM
                     KSGDGVLFYHSSCAEPGIAGLAEVASAAYPDPTQFDRKSKYYDAASKRDAPRWLLVDI
                     RARKKTRLIPLAELRSHAELADMAVLRRGNRLSITPVPAPEWAFIVRSLLKERA"
     gene            complement(9222..9403)
                     /gene="ssrS"
                     /locus_tag="VLN25_10030"
     ncRNA           complement(9222..9403)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VLN25_10030"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(9493..9792)
                     /locus_tag="VLN25_10035"
     CDS             complement(9493..9792)
                     /locus_tag="VLN25_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HSQ10934.1"
                     /translation="MEHLTVTILEREYRLACTPEEKDSLLRCAEYVDGKMQAIRSAGK
                     VMGADRIAVMAALQIAHELFSARSGDGIELGDLRRRVRDMLQSCDDALLPQEKLF"
     gene            complement(9780..10004)
                     /locus_tag="VLN25_10040"
     CDS             complement(9780..10004)
                     /locus_tag="VLN25_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF904 domain-containing protein"
                     /protein_id="HSQ10935.1"
                     /translation="MLEDFDLLAGKVAELARLVQSLRTENQQLRSQLTTASSELDAMR
                     GRVDEASRRLDLLMERLPTPPNSAGVPWNT"
     regulatory      10077..10292
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            10401..>12017
                     /locus_tag="VLN25_10045"
     CDS             10401..>12017
                     /locus_tag="VLN25_10045"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSQ10936.1"
                     /translation="MSHVRAQARRVALVTSPLLLAAGVSSAQPASELPPTVITPMRQS
                     QSIDDALPATSVITRAEIDRWQAVDIVSILSRETGVQFAQAGGRGAAASLFVRGANSS
                     QVLVLVDGVRLNAGTSGAAAVGGIALDSIDRIEIVRGNLSSLYGSSAIGGVVQIFTRR
                     GAEEGVVLSAEAGGGRTLNGRASAGVDAGPVRLGGAIGGGTTQSFSSIDPARVVTSAF
                     VPGANPDRDGNDYLSASLGATYRSGRGTLLSANGWINRNRTAFDSTADGAGATHLEKS
                     QVAAGNASARTTVTEGWVTQLAAGLSRDRSTNVVSDPMSFNNGEFSSDNLTVSWTNDV
                     DLTGAARVQVGVEYLRQDGESTSYDPSFSGQSQSFTRSVGSAWAGLSGAQGSHQFQLN
                     LRRDQYSDVDGATSALAAYGYKIDAAWRVTAQLSNAYRAPSFNDLYFPFFGNPYLEPE
                     RSASGELGLHYVGDRTTLRLAIYRTDTRDLIVYDPATARAENIDEARVTGFELGGSWR
                     EGPWQLSANGTVSRAINDDTGERLLRRASWV"
BASE COUNT         2010 a         3876 c         3987 g         2144 t
ORIGIN      
        1 tcggtgtccg cgacattgag ttcaagcagc aaccggtgcg cgtcgatcgt caccgagtcg
       61 ccttcctcga tcagtgcgat cgttccgcca acgtaagcct cgggcgctac gtgcccgacc
      121 accatgcccc aggtgccgcc ggacaaccgg ccgtcggtga tcaggcccac gctatccccg
      181 agtcccttgc cgatcagtgc tgacgtgggc gagagcattt cgcgcatgcc gggtccgccc
      241 ttggggccct cgtaacgaat caccagcacg tcgccggggc ggatgcggtc cgccaggatg
      301 gcctccatcg cggcatcttc cgagtcgaac acccgggccg ggcccgtgat gaccgggttc
      361 ttgagtccgg tgatcttggc cacgcatcct tcgggagaaa gattgccctt gaggatcgcg
      421 aggtgcccct gtgcgtacat cggccgtgaa aacggatgga tcacatcctg ggaggcggcc
      481 ggctctgcag ggacgtcggc cagcatcgcc gccatcgttt ttccggtgat agtcatgcac
      541 tcgccatgaa gcagtccgtt cgccaggagg accttcagta cctgcggcac gccgccggcc
      601 gagtgcaggt cggtggcgac atacttgccg gacggcttga ggtcgcacag caccggaacc
      661 ttgcggcgga tgcgttcgaa gtcgtcgatc gtccagggta tgccggcgga atgggcgatc
      721 gcgaggtagt gcagcactgc attggtgctg ccgccgaccg ccatgatcag gctcaccgca
      781 ttctcgatcg actcgcgcgt gatgatgtcg ctcggccgca ggttgttctt cacggccgcg
      841 atcagtacgc gcgccgcctc acgggtactg gtgaccttgt cctcgtccgg gttggcctgc
      901 gtcgatgaat acagcaggct catgccgagc gcctcgaacg acgaactcat cgtgttcgcg
      961 gtgtacatgc cgccgcagga accggagccc ggaatgcagc gccgctcgat ctccttgaag
     1021 tcggtctcgt tgaggcgccc cgcggtgaac tcgccgaccg cctcgaacgc cgagacgatg
     1081 gtcaggtcct tgtccttgta tcggcccggc ttcacggtgc cgccgtagac gtagatcgct
     1141 ggcacgttgc agcgggcgat gcccatcatg ccgccgggca tgttcttgtc gcagccgccg
     1201 atgacgatga cgccgtccat ccactggccg ttcacgcagg tttcgacgca gtcggcgatc
     1261 acctcgcgtg acaccagcga gtacttcatg ccttcggtgc ccatgccgat gccgtcggaa
     1321 atcgtgggcg tgccaaagac ctgcgagttg gccttgcagg cggcgatctc ctcgcacgcg
     1381 gcgtcggcca gcttctgcaa tccggagttg cacggcgtga tcgtcgagtg gccattggcc
     1441 acgccgatca tcggtttgtc gaagtcctcg tccttgtagc ccatcgcgta gtacatcgca
     1501 cggttggcgg agcgggcgac gccctgggtc acgtgctttg aacggtcgtt gtgcggcatg
     1561 ggcggtggct gaaagcaggc aggtgggcaa gcaggcaata ataggccaag ccatcattca
     1621 tacgcttcat tcgactgcga gacccgcgtg ccccccatcc atcacttcga ccccattgcg
     1681 ctgcagatcg ggcccctcgc catccgctgg tacgggttga tgtacctgat cggctttgcg
     1741 ctgttcctcg tgctgggcaa gctgcgttcg cgcgagacct ggcggggcat gagttcgcag
     1801 gacgtggacg acctgctctt ctacggaatg atcggcgtca tcgtgggtgg ccggctcggg
     1861 cacgtgatct tctacggtcc actgacgtac tacctgtccc atccgctcga gatcttcgcc
     1921 gtctggaagg gcggcatggc ctcgcacggc ggcatcctcg gcgtgatcgc tgcaatggcg
     1981 tggtttgcgc gcagccgcgg caagtccttc ttcggcgtcg ctgacttcgt cgtcccgctg
     2041 gtaccgctcg gactcgctgc ggggcgcatc ggcaacttca tcaacggtga actgtggggt
     2101 cgcgttgcgg atccggccaa cgttccgtgg gcgatgatct ttccgcaggc gggcgacaac
     2161 ctcccgcgcc atccgtcgca gttgtaccag ttcgccctcg aaggactgct gctgttcgcg
     2221 attctttggg tctactcgcg tcgaccgcgg cccgtcggca cggccggaag cctgtttctg
     2281 atcggctatg gagtgctgcg tatcgtcggt gagtttttcc gcgagcccga ggcactgtac
     2341 ggtgcgatgc cactgggcct gtcggccggg caatggctgt cggtgccaat ggtgctgatc
     2401 ggcatcgcct ttttcgtggt ctttcatcgc cggggactgt cgcctgtcgc ggccgcaaaa
     2461 cgctaaagta gcggacccga tcgccgggcc cgcgtgcggg acacgcacga aagagcatgc
     2521 gccgtcagag cgcagcatcc acattagcca aggagaaccc aggtgtactt tcgttccctg
     2581 atgattgccg tgcttgcggc cggtgtgctt gctgctgtgc cggcaagcgc ccaaagctgg
     2641 ccgaacaaac ccatcaccct gatcgttccg ttcccgcccg gtggtggcac cgacgccttc
     2701 gcgcggccgc tctcggcgca gctgaccaag caactcggcc ggcaggtaat cattgacaac
     2761 cgcgggggtg ccggcggcac ggtcggcgcc tcggcagcgg ccaaggctgc gccggacggc
     2821 tacaccttct tcgtcggtgc ggtccaccac gcgatcgcac cgagcttcta tccgaagctc
     2881 gactacaaca tcgagactga cctcgtaccg ctgacggtga tcgccaatcc cccacagatc
     2941 gtggtgatca atcccagccg ggtgaaggtc gaagatttca aggcgttcat gacactgctg
     3001 cgtgccaacc ccggcaaact caacttcgcc tcggccggca acggcacctc gcaccacctt
     3061 gcgggggaac tcttcaagca gcagagcaat accttcatcg tgcacatccc gtaccgaggc
     3121 gccgggccgg ccctgcagga cctgatcacc ggtaacgtcg acatgatgtt cgacggcctg
     3181 ggctcatcgg cccagcacat ccgcagcggt cgcatcaagg cgctggcggt ggctgcgcca
     3241 aagcgttcgc cggcgttccc gaacgtgccg accactgccg aggtcggtat gcccgactac
     3301 gtcgtgtcga cctggtacgg tgtctggggc gtgaagggca cgccgaagga gatccttgac
     3361 cgcatgcatg ccgaaatcgt caaggcggtc ggctcgcccg agctcaagga catctgggcc
     3421 ggcaacggct ccgacaccgc gacgatgtcg cagcaggact tcgcgaagtt cctgaacagc
     3481 gagatcaagc gctgggcggc tgtgacgaag tcgtccgggg ccaagctcga ctgagatcac
     3541 tcgtgagcgg cgtcgtacac ctgcgcagcg ccggcgagac ggcgacggtg gtcctcgaat
     3601 caccgggcaa gctcaatgcc atcagcgagt cgatgtggca cgcgctcgcc cgcgtgttct
     3661 cgacctttgc ggaagcgccg gggttacgcg ccgtcgtcat ccgcggcgcg ggacacaact
     3721 ttgccgcggg tgccgacatc gaggagttcg agcgcgtccg tggccagccc gccgacggac
     3781 gccgctatca cctggagacg atcgccaacg cgctggctgc gatcggagcg tgcccggtac
     3841 cggtcatcgc tgcgatcgag ggcgtgtgcg tcggcggcgg tctggaggtg gcgattgcca
     3901 gcgatctgcg cattgcggcc gacaatgcgc gcatcggagc gccggtcggg cggctcggtt
     3961 ttccgtttgc actgcccgaa ctcgtgccgc tgttgcgcct ggtggggccg ggtgtcgccg
     4021 ctgagctgct gctcgaggga cggctgtttg aagcgcacga ggcggccgcg cgcggtctcg
     4081 tcacgcgtgc ggtcgccctt gatcgcttcg acgcggaact gcgggagtgc gtgagccgca
     4141 tcgtcgccgg ctcgccgttg tcggcgcgcg agaacaaggc aaatattcgc cggctgaccg
     4201 ccacgggcgg cgtttatacc gagcgcgaac tggacgcgag tttcgcgttc ctgcagtccg
     4261 aggactaccg cgagggtgtt gcggcgtttc gcgccaagcg acccgcgcgc ttcaccggca
     4321 agtagtcgaa gtattcacct gccgcggggc ccggccatga cgagcgactc cagtttctat
     4381 tcgctgatcg aacggcgctt tcctgccgac ctcgattcag cctgtatcga gaccgccgac
     4441 ggactcttgt acagctggaa cgacctgcac catgcaagcg gacgcctggc ccactggctg
     4501 gactcattac ggttgcccgc cggcgcgcgg gttgcggttc aggtcgagaa gtcgcctgaa
     4561 tgcctgatgc tgtacctggc gaccttgcgt gccggcctgg tctatctgcc gctgaacact
     4621 gcgtaccagc gggcggagat cgaatacttc cttgccgacg ccgagcccga cgtgctggtc
     4681 tgcgcgccgg cccgacaagc cgaactcgaa ccgctggcgc gacgtgcgcg atgccaccat
     4741 gtcgtgacgc tgggcgaagc gcgcgagggc tcattgctcg aggccgccgc gccgtttggc
     4801 gacgtttaca cgacccgtgc tcgtgcagcg gccgacctcg ccgccatcct gtacacatca
     4861 ggcacgacgg ggcgcagcaa gggggccatg ctgacgcacg gtaacctggc gtccaatgcg
     4921 ctgaccctcg acgagttctg gggctttgcg gccgagcgcg aggcggggcg ccaggatgtg
     4981 ctgttgcatg cgctgccgtt atttcatgtg catggtctgt tcgttgcgtc gcatgcggcg
     5041 ctgctggccg gtgcgaagac gctgatgctg tcgaagttcg aggtcgcaga ggtgctcgaa
     5101 cggctgccac gctccacggt gatgatgggc gtgccgacct actacacacg gctgctggca
     5161 gagcccacct tcgacatggc gcgctgcaag tcagtgcggc tcttcgtctc cgggtctgca
     5221 ccgctgctgg ccgagacgca ccgcgagttt gaggcgcgca ccgggcaccg catcctcgag
     5281 cgctacggca tgtcggaaac cgtcatgctg gtatcgaacc cctacttcaa ggagcggggg
     5341 tcgcgcatcg cgggcacggt gggacagccg ctgccgggcg tcggtgtacg cgttgtgggc
     5401 gaggatggct ctgtctgccc gaccggggac atcgggtcgg tcgaggtgcg tggtccgaat
     5461 gtcttcgcag ggtattggcg catgccggag aagacggctg aagagttcac cgccgacggc
     5521 tggttttgca ccggcgatgt cggccatttt ggaggccgtg gcgtgcccga ccactacctg
     5581 acgctggtgg ggcgctcgaa ggacctgatc atctccggcg gcttcaacgt gtacccgaag
     5641 gagatcgagg gctacatcga cgagatgccg ggcgtggtcg aatcggccgt catcggactg
     5701 ccgcatccgg acttcggcga agcggtcgtc gccgtcatcg tggcgcgccg gccgggcgca
     5761 ttcgatgcga gaagcgtggt cgacggcctc aagggtcgga ttgccaacta caaggttccc
     5821 aagcgcgtct ttgtggtcga ggacctgccg cgcaacacga tgggcaaggt gcagaagaac
     5881 gtgctgcgcg atcgttacaa ggccaccttt acgccgcaaa cgcgaagtgc ggcctgaggc
     5941 tggtgccgcg gggtgaactg cggcccggca ttgccagcac cgcctcgacg aagccagact
     6001 tgccgttcgt ataggcgtcc ctgtcgaagc ggaagcgcga cgccaggcgg cgcttgagtt
     6061 cggcatagcg ggcagcgagc cacgcatcag cgcgcaggtt gtcgcggaac ttcacatacc
     6121 gcttccagta caggccgttg agctccacga cgtgcacgtg gtgtgtccgc acgtcgcgca
     6181 cgacccgctt gaagaaacgt cggtccggca gcacgcgttc gtattccgga atgtattcgt
     6241 agccatagat cgacaggcgg tcgtagtggc gctcgaactc gtcgagcgag cgcacgccta
     6301 ccagcatgtc gatgatgggc ttggccgcca ggcccggcac cgaggtgctt ccgatgtgtt
     6361 cgatcgcggg acgaaggtcg ctcagcgcga cctggatcag ctgtgactcg atgcgaaaac
     6421 gggcgggcca cgcagggtca tagggtgtca cgacgatctg gtcagtcatt cgagtctcct
     6481 tggagggcat cggggcgatc acgccggggc cgggtttgtg aggacactgt acgggggcgg
     6541 ggaatctgag gcagcggcgt ctgccgtcgc gccaatgccg caaattcgac aaacgggggt
     6601 ctttagcgcg ccagtcgggc agccggtccc atggcgcgct cgagcgaggc cagcacgatt
     6661 tcctcgggca gtggcgcctc gatcaacgca acgaaggccg cgccggccac gcagccagcc
     6721 agaagtacca ggaccaccgg caaccggctt tcgcggcgtc gcacgagagg acgtggctta
     6781 cgcaccactg cgggggtggg tagcgcggtc gggtcatgca tgacggcctc cgtgggcagg
     6841 gcgcttgcgc ccggtaagca cacggtaggc aactacggcg gcgagtgcag cggaggctgc
     6901 cgccgttgca acgacgaaaa ttcacgggcg cctgcggacg cctgcgcctg tcgacgggca
     6961 attgcgggcg tttgcggaag cccaggctcg ccgcttacca gcgtgccggc ggcttgcgat
     7021 ggatcgtgat ggtgcggttc tcgatcgtga cgtagccgcc gatggtcagg tccttgaaga
     7081 ttcggctgat catgtcgcgt gaagcgccaa cgcggtcggc aatgtcctgc tgcgtcatcc
     7141 gctcgggcac gaccagcttg cccccgtcct ctttcgccag cgacagcagc aggcgcgcaa
     7201 cccggccgta cacgtccatc agcgccaggt tcttcacgtt gtccgtcgca atgcgggcgc
     7261 ggtcgatcag cgttgtgatc agtgccatcg cgatatccgg attcgcaccg atcgcgctac
     7321 gcagttcgtc acgggtcacc accgagcagc tggtcggctc gagcgtcatc accgaagcgg
     7381 cgcgcggacg gccgtcgagc gccatctcgc cgacatagtc acccgggccg tagatgtcga
     7441 ggatcatctc gcggccgtcg tcatcggaca cgtagacctt gaccttgccc gtcaggatca
     7501 catagaggct gtcgccgcga tcgccctcgt tgatgatcac cgcgttctta ggaaacgtgc
     7561 gcacctgacc gcgtgtcgcc agttcgcgca ccatcggatc gccgatctcg ctgatggcct
     7621 gctcgcttgc tgtcatggtt gttccgccaa gtctcgttat cgcgccatta tgcggcgcac
     7681 ggcagtggcg gggaaggtgc cggggggcgg cccgttccgc gtttgccgtt gctagtacgc
     7741 ccgggcgccc ttgtacgcca tgtagcccgc cagcaggaac agcaggatgg caaaaacgcg
     7801 tttcagcggc ttggtatcga gcgagtgggc aacgcgcgcc cccagcggcg ccgtcagtac
     7861 gctggcagcg gcgatagcca gcagcgccgg cagatagacg aaaccggcgg tgcccggagg
     7921 cagtgacgcc tcgccgtggc cggcgaggat gtagccaagg gttccggccg cggcaatcgg
     7981 gaatccgagg gcggccgagg tcgcaacggc attgtggatc ttcacgttgc tccagaccat
     8041 gaagggcacg gaaatgaatc ctccgccggc gccgaccagg ctcgaaatga atccgatgcc
     8101 gctgccgaca ccgaacatgc ccgcaccctt cggcagttcg cgtgtcggtc gcggctttcg
     8161 gtcgagcagc atctgcagcg cagaaaatcc gacgaaggtt gcgaacacga ccgacagcca
     8221 gaaggtcggc agtcgtcccg cgatctgggc accgatcagc gatccgagca ggatgccagg
     8281 cgcaagcaac cggacgaccg gccacaggac ggcgcctcgc tggtggtgcg cacgcacgga
     8341 cgacaccgag gtgaacaaaa ttgtcgcgag cgacgtggcg atcgccatgt ggacgatgtg
     8401 ctcgagcgga aagccacgcc acgtgaacag cagcgtcagg aacggcacca tcagcatgcc
     8461 gccgccgatg cccaaaaggc ccgcggcaaa gccggttccc gcgcccagca gcaccaatgc
     8521 agcaatgaac cccacctcca ttacgcgcgc tccttgagca gactgcgcac gatgaacgcc
     8581 cactccggcg cgggcaccgg agtgatcgac agtcggttgc cgcgccttaa taccgccatg
     8641 tcggccagct cggcgtgcga tcgcagttcg gcgagcggga tcagccgcgt cttcttgcgt
     8701 gcgcggatgt ccacgagcag ccagcgcggc gcgtcgcgct tggatgcggc gtcgtagtac
     8761 ttgctcttgc ggtcgaactg ggtcgggtcc gggtaggcgg cgcttgcgac ctcggccaga
     8821 ccggcgatgc cgggctccgc acagctcgag tgatagaaaa gcacgccgtc gccggacttc
     8881 atcccgtcgc gcatgaagtt gcgtgcctgg tagttccgca cgccggtcca cggcacggtc
     8941 ttcttcggcg ccgccagcgc gtcgtcgatc gagcactcgt ccggttcgga cttcatcagc
     9001 cagtagctca tgcgcggccc acgtggccgt gggatccaca cggcggactc gccgccgacg
     9061 ctggcagcac aggaatgatc atcggaaggt cagtggatgg aatgcggaac gccgtcctgg
     9121 ggcggtacgc aggccgctgt cgcaggatgg aatcgcgctg cgccggcact cgccgctcgc
     9181 agactagcag cgcctgggtc gccagtgaac gcgacccgaa taagatcccc gcctgtgccg
     9241 cccggcctga tgtcctgaac catcggttca ggttggtcac tgtccgcgtc gtttaggttc
     9301 agcaaacgcg tgccgatcgc accgcgacgc gagcccagca accttgcgca tagcgcatcg
     9361 gttcaaggaa ctatcggcct ttgcgcgggc accgcaggga aaccctttgt gcattcgcga
     9421 acatcggact ctgcgcgaat gcacttcaat ttcattgtaa cgccgctcct gagttcttcg
     9481 ttgaattcag cgctagaaaa gtttttcctg cggcagcaat gcgtcgtcgc aggattgcag
     9541 catgtcgcgc acgcgacggc gcaggtcacc gagctcgatg ccatctccgc tgcgggccga
     9601 gaacagttca tgcgcgatct gcaaggccgc catcacggcg atgcggtccg cgcccatgac
     9661 tttgcctgcc gagcggatcg cctgcatctt gccgtcgacg tactccgcgc atcgcagcag
     9721 cgagtccttt tcctccggcg tgcaggcaag gcggtactcg cgctcgagaa tggtgacggt
     9781 caggtgttcc atgggacccc agcggaattc ggcggcgtcg gcagccgctc catcagcaga
     9841 tcaaggcgcc ggctcgcctc gtcgacgcga ccgcgcatgg cgtccagctc ggacgaagcc
     9901 gtggtcagct gcgagcgaag ctgctggttt tcggtgcgca gcgactgcac cagccgtgca
     9961 agttcggcca ccttcccggc gagcagatcg aagtcttcaa gcattgaata atcgtagcac
    10021 tgcaccttgc agccgatcgg ccggactagg gcctcgcgcg cggactttcc tgggctagac
    10081 ttcctcgcgt tgatggtgct gaaggccacg ccttcggtga aacgggaaac agcaggtgcg
    10141 ccgctgtcag gcgcgcccaa gctgtgctgc ccccgcaacg gtaagcaggc gcgcattgcg
    10201 cacgcttcgc acatgccact gcgtctcgcg acgcgggaag gcgcgaagcg caacctgcag
    10261 cccggagacc ggccatcagc aggaggacga gccgcggggg accggcaagc cggaccgcgt
    10321 cgtgcgcgcc ctgctcagcc cactcccggc cgttcgagtc tgaacgactg ccggcggagg
    10381 gccggcgcag gggagttcgc atgagtcatg tccgggcgca ggcgcgccgc gtcgcgctgg
    10441 tgacgagccc acttttactg gccgccggtg tgtcgagcgc acagccggcg tccgagcttc
    10501 ccccgaccgt catcaccccg atgcgtcaat cgcagtcgat cgatgacgcg ttgccggcca
    10561 catcggtcat cacgcgcgcc gagatcgatc gctggcaggc ggtcgacatt gtgtcgatac
    10621 tgagtcgcga gaccggcgtg cagtttgcgc aagccggtgg tcgcggcgca gcggcgtcgc
    10681 tcttcgtacg aggcgcgaac tcatcccagg tactggtcct ggtcgacggc gtgcggttga
    10741 atgcaggcac cagcggcgcg gcggccgtcg gtggcattgc gctcgactcg atcgaccgca
    10801 tcgagatcgt gcgcggcaac ctgtcgagtc tctacgggtc gtcggcgatc ggcggtgtcg
    10861 tgcagatctt cacgcggcgc ggcgccgagg agggtgtggt cctcagcgcc gaggcgggcg
    10921 ggggccggac cctcaatggc cgtgccagtg ccggagtcga cgccggtccg gtccggctcg
    10981 gcggcgccat cggcggcggg accacgcaat cgttctcctc gatcgacccg gcacgagtcg
    11041 tgacaagcgc tttcgtaccc ggcgcgaatc cggatcgcga cggcaacgac tatctgtccg
    11101 cttcgctggg cgccacttac cgatcgggcc gcggcacctt gctgagcgcg aatggctgga
    11161 tcaaccgcaa ccgcacggca ttcgacagca ccgccgacgg ggcgggtgcg acgcacctgg
    11221 aaaaatcgca ggtggcagca ggcaatgcga gtgcccgtac gacggtcacg gaagggtggg
    11281 tgacgcagct tgcggccggc ctgagccgtg atcgcagcac caacgtcgtc agtgatccca
    11341 tgtcattcaa caatggtgag ttcagctctg acaatctcac ggtgagctgg accaacgacg
    11401 tagatctcac gggggccgcg cgtgtgcagg tcggcgtcga gtatctgcgc caggacggtg
    11461 agtcgacgtc ctacgacccg tcattcagtg gccagtccca gagtttcact cgctctgtcg
    11521 gaagtgcttg ggccgggctg agcggagctc aggggtcgca tcagttccag ctgaatctgc
    11581 gccgcgacca gtactcggat gtcgatggcg ccaccagcgc acttgccgct tatggctaca
    11641 agatcgatgc tgcatggcgc gtgactgcgc aactgtcgaa tgcgtatcgt gcgccgagct
    11701 tcaacgacct ttactttccc ttcttcggca atccctacct tgaacccgag cggtcagcca
    11761 gcggtgaact gggcctgcac tacgtcggcg acaggacgac gctgcgcctg gcgatctacc
    11821 gcaccgacac gcgtgatctg atcgtgtacg accctgccac cgctcgcgcg gagaacatag
    11881 acgaggctcg cgtgactggt ttcgaactgg gtggcagctg gcgcgaagga ccctggcaac
    11941 tgtccgccaa cggcacggta tcgcgcgcca tcaacgatga cacgggcgag cggctgctgc
    12001 gtcgcgcgtc gtgggtg
//
LOCUS       DATIRT010000443         2848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1059244, whole genome shotgun sequence.
ACCESSION   DATIRT010000443 DATIRT010000000
VERSION     DATIRT010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059244"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..901)
                     /gene="creB"
                     /locus_tag="VLN25_10050"
     CDS             complement(191..901)
                     /gene="creB"
                     /locus_tag="VLN25_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HSQ10937.1"
                     /translation="MKPRILVVEDEPAIADTIVYALSTDGFEPAWCATGAAALTAVSE
                     RPAALAILDVGLPDINGFELFRRLQQVDPALPAIFLTARSSEIDRVVGLELGADDYIG
                     KPFSPRELVARVRTVLRRVQRVPVAPATPGAPVVQAQGAFTVDDEKKTIRFRGRPLDL
                     SRTEYRLLKVLIERPGRVYSRDELMQRAWDDPASAFDRTVDAHVKALRAKLRDVDGSV
                     DPISTHRGMGYSLREDLA"
     gene            complement(898..2091)
                     /locus_tag="VLN25_10055"
     CDS             complement(898..2091)
                     /locus_tag="VLN25_10055"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HSQ10938.1"
                     /translation="MHTTRPGAVRQLWIALFAAALMAVGVVLALRAMSFDSEMHHRYE
                     SALTLSQAAIEAQQASADLRSWQARNARELLRDGDAALPPSELAALVQASVELRSRLA
                     RLADLDMPPERKARIAELQAKLSGFEQLGQGTTSRSGTGSAGSQISRSALVSSSVEFD
                     AFEHSMTELVVGTNARAAAIARQAVGLNETARTGLIAFSGITLVLAVLLIGLTLRTLR
                     SNNVLLDQLSHLAQEDALTGIANRRTLDEALPVEFARALRSGQPLTLVMLDFDHFKRY
                     NDRRGHAAGDALLRGAAQAWSKQMRPTDLLARYGGEEFTLVLPACTADQAAQLVDRLR
                     PLMPDRQTFSAGIATWEGHETATELLQRADRALLLAKKGGRNRTMIAGRELQATLPLE
                     VAATS"
     gene            complement(2211..2549)
                     /locus_tag="VLN25_10060"
     CDS             complement(2211..2549)
                     /locus_tag="VLN25_10060"
                     /inference="COORDINATES: protein motif:HMM:NF020469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HSQ10939.1"
                     /translation="MSLVVFRSKAAGEIFMFAETARRLLEILGRGEAPRGVITADQVP
                     DALRRLEAAVEQEKADIKAAASAGDQNERRGPDGPAAPAPITLGQRAFPLIEMLRAAA
                     KRNVDITWGV"
BASE COUNT          470 a          943 c          984 g          451 t
ORIGIN      
        1 ctggcgctcg agcagcgttg ccagcaggtt ggccgtgtcg acaagcgcgt cctcggccga
       61 ctcgcggtag cgcggatcga ggtcgaggct gacgcggtac agcaggaaga cgacggccgc
      121 gatgtagacg acgaccacgc cgagaaacat gcgtgaacgc cgtgacatca gcgcttccgc
      181 agtggcccgg tcaagcgagg tcctcgcgca gcgagtaacc catgccgcga tgggtcgaga
      241 tcggatcgac cgatccgtcc acgtcgcgca gttttgcgcg aagcgccttc acgtgggcat
      301 cgaccgtgcg gtcgaatgcc gaggccgggt catcccaggc gcgctgcatc agttcgtcgc
      361 gcgagtagac gcggcccgga cgttcgatca gcacttttag cagccggtac tcggtgcggg
      421 aaaggtccag cggtcgcccg cggaaccgga tcgttttttt ctcgtcgtcg accgtgaacg
      481 cgccctgcgc ttgtacgaca ggtgcgccag gggtcgcggg agccacggga acccgctgca
      541 cgcgacgcag cacggtgcgc acgcgagcca ccagttcgcg aggcgagaac ggcttgccga
      601 tgtagtcgtc ggcgccgagt tcgagaccga caacgcggtc gatttcgctc gagcgcgcgg
      661 tcaggaagat ggccggcagt gccgggtcca cctgctgcag ccgcctgaag agttcgaaac
      721 cgttgatgtc gggcagcccc acatcgagaa tggcaagcgc ggccgggcgt tccgataccg
      781 ccgtgagcgc cgctgcgccg gtggcacacc atgcgggctc gaaaccgtcc gtggacagcg
      841 cgtagacgat ggtatccgcg atcgcgggct cgtcttcgac caccagaatg cgcggtttca
      901 cgaggtggcg gcgacttcga gcggcaaggt cgcctgcagc tcgcggccgg caatcatcgt
      961 ccggttgcgt ccgcctttct tggccagcag caacgcacgg tcggcgcgct gcagcagctc
     1021 ggttgcggtt tcatgaccct cccaggtcgc gatgcccgcc gagaacgtct gccggtccgg
     1081 catcaacggc cgcagccgat cgacgagctg ggcggcctgg tcggcggtgc atgccggcag
     1141 cacgagcgtg aactcttcgc cgccgtagcg cgccagcaga tcggtgggac gcatctgctt
     1201 gctccaggcc tgggccgcgc cccgcagcag ggcatcgccg gccgcgtggc cgcggcggtc
     1261 gttgtagcgc ttgaagtggt cgaaatccag catcaccagc gtgagcggct ggccgctgcg
     1321 caacgcgcgg gcgaattcca ccggcagggc ctcgtcgagc gtgcggcggt tggcgatgcc
     1381 ggtgagcgcg tcttcctgcg ccagatgcga cagttgatcc agcagcacgt tgttcgatcg
     1441 cagcgtgcgc agggtgagcc cgatcagcag gactgctagc accagcgtga tgccggaaaa
     1501 ggcgatcagt ccggtacggg cggtctcgtt cagcccgacg gcctggcgcg cgatggccgc
     1561 cgcccgggcg ttcgtcccga ccacgagctc ggtcatggag tgctcgaacg cgtcgaactc
     1621 gacactcgag gaaaccagcg ccgagcgcga aatctgcgaa cccgcggatc ccgtcccgct
     1681 gcggctcgtc gtgccctggc cgagttgctc gaagcccgaa agtttcgcct gcaattcggc
     1741 gatcctcgcc ttgcgctccg gtggcatatc gaggtcggcc aggcgggcca ggcggctgcg
     1801 aagctctacc gacgcctgca caagggctgc gagctcggac gggggaaggg cggcgtcacc
     1861 atcgcgcagc agttcgcggg cgttgcgcgc ctgccaggaa cgcaagtctg cgcttgcctg
     1921 ctgcgcctcg atcgccgcct gcgaaagcgt cagcgcggat tcatagcgat ggtgcatttc
     1981 gctgtcgaat gacattgcac gcaacgcgag cacgaccccg accgccatca gcgcggcagc
     2041 aaacagcgcg atccacaact ggcgaacggc tccgggacgg gtggtgtgca agctcgggtc
     2101 tcggcgattg gaggacgaac gtgacaattc tagggcgggg cccgatcacc cgacggtgga
     2161 tctgtccgat gacatccgtg ccgcgactgg cggagcgacc ctgaatgccg ctagacgccc
     2221 caggtgatgt cgacgttgcg cttcgccgcg gcgcgcagca tttcgatcag ggggaacgcg
     2281 cgctggccca gcgtgatggg cgccggcgcc gcgggcccgt ccggcccgcg ccgttcgttc
     2341 tgatcccccg cggacgcagc cgccttgatg tcggcctttt cctgctcgac ggcggcctcc
     2401 aggcggcgca gcgcgtcagg cacctggtcg gccgtaatga cgccgcgcgg cgcctcgcct
     2461 cggccgagga tttctaagag tcgtcgggcg gtctcagcga acatgaagat ttcgccagcg
     2521 gccttggagc gaaatacaac tagggacatg agcggaagga cggccgaatc cgtcacggaa
     2581 ttgtcgactc ggactcgcgt tcgccgcgac cagcgcccac ggcaacttgc cattgggctt
     2641 tgattgtagg ccgaccgggt tcgccgaccg ggcttaccgg cccagtttgc cgaaatacgc
     2701 aacggcgcgc taggagcgcc tccgaccgcg ctgccaccgg cggccggctg gagctgccga
     2761 tcagtctcgc ttgtcgtcgc gcgagaccgt ggccttcacg acgtcgtcga cgtcgcgccc
     2821 ataaaccagc ggcagcgaag caatcacg
//
LOCUS       DATIRT010000444         2445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1550906, whole genome shotgun sequence.
ACCESSION   DATIRT010000444 DATIRT010000000
VERSION     DATIRT010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550906"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VLN25_10065"
     CDS             <1..438
                     /locus_tag="VLN25_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HSQ10940.1"
                     /translation="VQAEAAIRHLASSYAYDEGENENSDEPTLRASQDRVAQALIREL
                     TDRFDQAGINVEDAKLTHLAYAPEIAQVMLRRQQAEAIIAARSKIVLGAVSMVEMALK
                     GLSERGIVDLDDERRAAMVSNLLVVLCAESEVQPVINTGTLYP"
     gene            454..639
                     /locus_tag="VLN25_10070"
     CDS             454..639
                     /locus_tag="VLN25_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10941.1"
                     /translation="MNPFLLWVPGVALIVLGALLLAIGTLGTTAALVLIGIGVTIESA
                     GVLLWVRDRSRKGARPR"
     gene            687..920
                     /locus_tag="VLN25_10075"
     CDS             687..920
                     /locus_tag="VLN25_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10942.1"
                     /translation="MSYEHTQRGLAVRIATLLGAVGMVAGVVFSPEELPRHLYTVLLL
                     VAAAIALVGWSFSVLTVRVGAGELHWHFGSGWP"
     gene            933..1157
                     /locus_tag="VLN25_10080"
     CDS             933..1157
                     /locus_tag="VLN25_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10943.1"
                     /translation="MERIADVDLTRTTFWEGWGVHRTRRGWLYNVAGQDAVLVRRSDG
                     TAVLLGSDEPRKLKDALQAAMRAASDLRKA"
     gene            1189..1374
                     /locus_tag="VLN25_10085"
     CDS             1189..1374
                     /locus_tag="VLN25_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10944.1"
                     /translation="MASPEKKSFLLRIDPRLWADLERLAAADLRSVNAQIEFLLREAL
                     ERRGLRTQVDARRKAGR"
     gene            complement(1411..>2445)
                     /locus_tag="VLN25_10090"
     CDS             complement(1411..>2445)
                     /locus_tag="VLN25_10090"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HSQ10945.1"
                     /translation="LADTKLKRWDVNFLKERVRKERYSVDQEALRAYFPTEASVQYAM
                     QLADRLYSVEFVARDVPRWHSDVRYYDVYDRLPDGKRGAFVGGIYLDLFPRDGKYNHA
                     AAFPVRGVSTLANRTPISVLVANLNAKGLNHEELETLLHEYGHVLHGVLSKTRYVDQS
                     GTSVKRDFVEAPSQMFEEWARRDEPLALFAQICPTCPRLTREQISQLEAARKFGRGNF
                     FARQGEYALYDMRLHTGTPGEPMPPWIAIENGGPLGFVDGSLFPAGFGHLLGGYAAGY
                     YGYMWSQVIALDMLSAFDSKLMNPAVGQRYRKTILASGGQRPPHALVEAFLGRKPNSD
                     AFYAEINGTR"
BASE COUNT          421 a          774 c          828 g          422 t
ORIGIN      
        1 gtgcaggccg aggcggcgat ccgccacctg gcgtcgagct atgcctatga cgaaggcgaa
       61 aacgagaact cggacgagcc cacgctgcgc gcaagccagg atcgcgtggc gcaggcgctg
      121 atccgcgaat tgacggaccg cttcgatcag gccggtatca atgtcgaaga cgcgaagctc
      181 acgcacctgg cctatgcccc ggaaatcgcc caggtgatgc tgcgccgtca acaggcggag
      241 gcgatcattg cggcgcgatc caagatcgtg ctgggcgctg tcagcatggt cgaaatggcg
      301 ctgaagggtc tgtccgagcg aggcatcgtt gacctcgacg acgagcggcg cgcggccatg
      361 gtcagcaacc tgctggtcgt gctgtgcgcc gagagcgagg tacagccggt gatcaacacc
      421 ggcacgctgt acccctaggc gcggcgtccg gcgatgaatc cgttcctgct ctgggttccg
      481 ggcgtcgcgc tgatcgtgct cggagcgttg ctgctcgcca tcggcactct cggcacgacg
      541 gccgccctgg tcctcatcgg catcggggtg acgatcgaga gtgctggagt cctgctctgg
      601 gtgcgagatc gctcgcgcaa gggcgcgcga ccgcgatgaa ccgatgaact acgacgaacc
      661 tattcgatga cccacgaaca tattcgatga gttacgaaca cacacaacgc ggcctggcgg
      721 tgcgtatagc gacgctgctc ggcgccgtcg gcatggtcgc cggtgtcgta ttcagtcccg
      781 aggagcttcc gcgccatctg tacacggtcc tgctgctggt cgctgcagcc atcgccctgg
      841 tgggctggtc attcagcgta ctgacggtgc gggttggcgc cggcgaactg cactggcact
      901 tcggctcggg ctggccgtag tggtcgacac cggtggagcg catcgcggat gtcgacctga
      961 cgcgcacgac cttctgggaa ggctggggcg tgcaccgtac gcggcgcggc tggctttaca
     1021 acgtcgcggg acaggacgcc gtgctggtgc ggcgcagtga cggcaccgca gtcctgctgg
     1081 gcagcgacga gccgcgcaag ttgaaagacg cgttgcaggc agccatgcga gccgcgtccg
     1141 atctgcggaa agcgtaacga caggatcgac aggacagaca ggaccggcgt ggcgagcccc
     1201 gaaaagaagt ccttcctgct gcgcatcgac ccgcgattgt gggccgacct ggagcggctc
     1261 gctgcggcgg acctgcgctc ggtcaacgca cagatcgagt tcctgctgcg cgaagcactc
     1321 gagcgccgcg gccttcggac ccaggtcgat gcgaggcgca aagccgggcg ctgaaattgc
     1381 cgcccggctg gccgaccgtc agagcgcggc tcagcgcgta ccgttgatct cggcgtaaaa
     1441 cgcgtcgctg ttcggcttgc gtccgagaaa ggcctcgacc agcgcatgcg gcgggcgctg
     1501 ccctcccgag gccaggatcg tcttgcggta gcgctggccg accgcgggat tcatcagctt
     1561 gctgtcgaac gccgacagca tgtcgagcgc gattacctgc gaccacatgt atccgtagta
     1621 gcccgccgca tagccgccaa gcaggtggcc aaaccccgcg gggaagagcg atccgtccac
     1681 gaatccgagc ggccccccat tctcgatggc gatccagggt ggcatcggct cgccgggcgt
     1741 gccggtgtgc agacgcatgt catacagcgc gtactcaccc tggcgtgcaa agaagttgcc
     1801 gcgcccgaac ttgcgggcgg cttcgagctg ggaaatctgc tccctggtca gtcgcgggca
     1861 tgtcgggcag atctgcgcga acagcgccag cggctcgtcg cgccgcgccc attcctcgaa
     1921 catctgtgac ggggcttcga cgaagtcgcg cttcactgac gtgccggact ggtcgacata
     1981 gcgcgtcttc gacagcacgc cgtgcagcac gtggccgtac tcgtgcagca gcgtctcgag
     2041 ttcctcgtgg tttagccctt ttgcattgag gttggccacc agcaccgaga tcggcgtgcg
     2101 gttcgccagc gtggaaacac ctcgtaccgg aaacgcagct gcatggttgt atttgccatc
     2161 gcgcgggaac agatcgaggt agatgccgcc gacgaaagcg ccgcgcttgc cgtcgggcaa
     2221 tcggtcgtag acgtcgtagt agcgcacgtc gctgtgccag cgcggcacat cgcgcgcgac
     2281 gaactcaacg ctgtacaggc ggtccgccag ctgcatggcg tactgcacgc tggcttcggt
     2341 cgggaagtac gcgcgcagtg cttcctggtc gacgctgtag cgctccttgc gcacgcgctc
     2401 tttcaggaag ttgacgtccc agcgtttgag tttcgtgtcg gcgag
//
LOCUS       DATIRT010000445         1914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1059327, whole genome shotgun sequence.
ACCESSION   DATIRT010000445 DATIRT010000000
VERSION     DATIRT010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059327"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1784)
                     /gene="soxB"
                     /locus_tag="VLN25_10095"
     CDS             complement(72..1784)
                     /gene="soxB"
                     /locus_tag="VLN25_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HSQ10946.1"
                     /translation="MDRREFLQLLAVAAAAGMPIGARDALAATPASAKMYELPRFGNV
                     HLLHFTDCHAQLLPIYFREPSVNLGIGPARGQPPHLVGEHLLKHFGIARNTPEAHAFT
                     FLDFVEAAQTYGKIGGFAHLSTLIKRMKAGRPGALLLDGGDTWQGSATSLWTNAQDMV
                     DACKLLGVDVMTAHWEMTYGQKRVLEIVEKDFKGRIDFVAQNIKTTDFGDQVFAPYTL
                     REMNGVKVAIIGQAFPYTPIANPRYFVPEWSFGIQEENMQKVVDEARGKGAQVVVVLS
                     HNGMDVDLKMASRVRGIDAILGGHTHDGVPQPSMIRNAGGTTLVTNAGSNSKFLAVLD
                     FDVKGGKVADFRYKLLPVFSNLLPADADMSAFIQKVRAPYEGKLSEKLATTEGLLYRR
                     GNFNGTFDQLILDAMLAVKGAEIGFSPGFRWGTSLLPGQAITMEHLMDQTAVTYPYST
                     VSDMSGEMIKTVLEDVADNLFNPDPYLQQGGDMVRVGGLQYTIDPAAAMGGRISDMRL
                     NGKPIETGRTYKVAGWAPVAEGAKGEPIWDVVAGYLRERKVVAPLRLNQPVIKGVAGN
                     PGIA"
BASE COUNT          320 a          631 c          600 g          363 t
ORIGIN      
        1 gcaccctgcg ccccccgcca gggcagcagc agggcggcgc acaacaggtg gcgccgtcgc
       61 atggcggtgg gtcaggcgat gccggggttg cccgcgaccc ctttgatgac cggctggttg
      121 agccgcagcg gtgcgacgac cttgcgttcg cgcagatagc cggcgacgac atcccagatc
      181 ggctcgccct tggctccttc cgcgaccggc gcccagccgg ccaccttgta cgtcctgccg
      241 gtctcgatcg gtttgccgtt gagccgcatg tcgctgatac gaccgcccat ggccgccgcg
      301 gggtcgatcg tgtactgcag gccccccacg cgcaccatgt cgccaccctg ctgcaggtag
      361 gggtccgggt tgaacaggtt gtcagcgacg tcctcgagga cagtcttgat catctcgccg
      421 ctcatgtcgc tgaccgtcga atacggatac gtgacggcgg tctggtccat caggtgttcc
      481 atcgtgatcg cctggcctgg cagcagactg gtgccccagc ggaatcccgg cgaaaaaccg
      541 atctcggcgc ccttgacggc cagcatcgca tcgaggatca gctggtcaaa cgtgccgttg
      601 aagttgccgc ggcggtacaa caggccctcg gtcgttgcga gcttttcgga aagcttgcct
      661 tcgtacggcg cgcgcacctt ctggatgaag gcactcatgt ccgcatcggc gggcagcaga
      721 ttcgagaaca ccggcagcag cttgtagcgg aagtcggcga ccttgccgcc cttgacgtcg
      781 aagtccagca ccgcgaggaa cttgctgttg ctgcccgcat tggtgaccag cgtagtgccg
      841 ccggcattcc tgatcatgga cggttgcggc acaccgtcgt gcgtatggcc accgagaatc
      901 gcatcgatgc cgcgcacgcg cgaggccatc ttcaggtcga catccatgcc gttgtgcgaa
      961 aggacgacga cgacctgcgc gcccttgcct cgcgcctcgt cgaccacctt ctgcatgttc
     1021 tcttcctgga tgccgaagct ccattcggga acgaagtagc gcggattggc gatcggcgtg
     1081 tacgggaacg cctgcccgat gatggcaacc ttcacgccgt tcatctcgcg cagcgtgtac
     1141 ggcgcaaaga cctggtcgcc gaagtccgtc gtcttgatgt tctgcgcgac gaagtcgatc
     1201 ctgcccttga aatccttctc gacgatctcc agcactcgct tctggccgta ggtcatctcc
     1261 cagtgcgccg tcatcacgtc tacaccgagc agcttgcagg cgtccaccat gtcctgcgcg
     1321 ttggtccaca gggatgtcgc cgagccctgc caggtatcgc cgccgtcgag cagcagcgca
     1381 cccggtcgcc cggccttcat acgcttgatc agggtcgaca aatgggcgaa gccgccgatc
     1441 ttgccgtagg tctgggccgc ctcgacgaag tcgaggaagg tgaatgcgtg cgcttccggc
     1501 gtattgcgcg cgatgccgaa gtgcttgagc agatgttcac caacaaggtg cggcggttgg
     1561 ccgcgcgcag gaccgatgcc gaggttgacg ctcggctcgc gaaagtaaat cggcagcagc
     1621 tgtgcatggc agtcggtgaa gtgcaacagg tgcacgttgc cgaagcgcgg cagttcatac
     1681 atcttcgcgc tggcaggcgt cgcggccagc gcatcgcgcg cgccgatcgg cattccagcc
     1741 gcggccgcga cggccagcaa ctgcagaaac tcacgtcggt ccatcgacat cgtgcgtacc
     1801 tctccctgtc cgttctctgg tgtcttgttc gaactacggc gacctggcgg cgaagcgcgc
     1861 cagcgctgcg atgtcttcct cgaacatctc gccgatctcg ctgaagacga tgcg
//
LOCUS       DATIRT010000446         3142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_272176, whole genome shotgun sequence.
ACCESSION   DATIRT010000446 DATIRT010000000
VERSION     DATIRT010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272176"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1211
                     /locus_tag="VLN25_10100"
     CDS             <1..1211
                     /locus_tag="VLN25_10100"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSQ10947.1"
                     /translation="ATGVPLAELPPVTKRELMDDFDAVVTQDGVTRHAVEQFIAEPER
                     IGQALDGRFGVWTSSGTTGTPGYFIHDPDAMAVYDALEAQRFRGVRSPTDLVRQLMHG
                     DRYAMVAATDGHYAGVASVERMRQWAPWMADAVRAFALLQPCSQLVLQLNAYRPTILA
                     TYPTAAEMLAEEQEAGRLRLRPREIWTGGEVLSEPTRLRVQRAFGCRVRSAYGASEFL
                     PIAWDCLHRRLHVNADWVVLEAVDRLGRPVEPGTRSHSVLLTNLANRVQPLIRYDLGD
                     AVTVHPDRCACGSHFPSVSVEGRCDDTLRLPGRRGGEVMLVPLALETVLEEGAGAHEF
                     QVVQRADGALTIHLGAQDQRRRGPVRAALRRYFETCDVGPVEILISRRGPTRDPASGK
                     LRRVICRLAH"
     gene            1253..2347
                     /locus_tag="VLN25_10105"
     CDS             1253..2347
                     /locus_tag="VLN25_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HSQ10948.1"
                     /translation="MSLRAPIAWFRRHPVLWLFGAALLLVGLGAGCSTFGYYAQSIQG
                     HMAVMEAARPIPDVLDDPNAAVPLKQRLERVRLIRAFASSDLGLPDNGSYTRYADLKR
                     PYVVWNVFAAPELSLQLKQWCYPVVGCAGYRGYFEQAAADAAAAELRSTGYEVNVAGV
                     PAYSTLGWFSDPLLNTFIGGSEGQVAGLVFHELAHQVVFVGGDTTFNESFATAIERYG
                     VKRWLDARGDDATRRAYAEFALRRSQFLELLLKYRKLLQENYSSGVPDEVKRARKQHL
                     LSELKQEYARLKESWGGFAGYDRYFAQDLTNAHLAAVAAYNDLVPAFDALLVQSKGNM
                     PAFYSEVKRIAALPKAERDAALTRLQTSTR"
     gene            2478..>3142
                     /locus_tag="VLN25_10110"
     CDS             2478..>3142
                     /locus_tag="VLN25_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSQ10949.1"
                     /translation="MDRFWLKNYPKGVPADIDYHQYKSLVQLLEEAFKKYSGQPAYSC
                     MGKALTFGDLDIQSAAIGAWLQSRGFTKGKRVAIMMPNVLQYPVVLAGVLRAGCTVVN
                     VNPLYTPRELEHQLKDSGAEAIFILENFATTLQQVADKVPTKTVVVCAMGDMLGFAKG
                     LLVNFIVRNVKKMVPEYTLANALRWNEVLADGLRMSLKPVDVTLNDTAFLQYTGGTTG
                     VSKG"
BASE COUNT          533 a          982 c         1056 g          571 t
ORIGIN      
        1 ccgctaccgg ggtccccctt gctgaactgc cgccggtcac caagcgcgaa ctgatggacg
       61 acttcgacgc ggtcgtcacg caggacggcg tgacgcggca cgcggtcgag cagttcatcg
      121 cggagccaga gcgaatcggg caagcgctgg acggccgttt tggcgtctgg acaagttcgg
      181 gcaccacagg cacgccaggc tacttcattc acgatccaga tgcgatggcc gtgtacgacg
      241 cgctcgaggc acagcgcttc cgcggagtgc gctcgcccac ggatctcgtg cggcagctga
      301 tgcacggcga tcgttatgcc atggtggccg ccaccgacgg tcactacgcg ggggtcgcga
      361 gcgtcgaacg gatgcgccaa tgggcgccgt ggatggccga tgccgtgcgt gcgtttgcgt
      421 tgttgcagcc gtgttcgcaa ctggtgctgc agctgaatgc gtaccggccg acgatactgg
      481 ccacctatcc cacagcggcg gaaatgctgg ccgaggaaca ggaggccggg cggttgcgct
      541 tgcgaccgcg cgagatctgg accggtggtg aagtgctttc ggagccgacg cgactgcgtg
      601 tgcagcgggc cttcggttgc cgtgtacgca gtgcctacgg tgcgtcggaa tttctgccga
      661 tcgcctggga ttgcctgcat cgccggctgc acgtgaacgc cgactgggtc gtgctggaag
      721 ccgtagaccg gctgggccgg ccagtcgagc ccggaacgcg ttcgcattcg gtgctgctga
      781 ccaacctggc gaaccgcgtt cagccgctga tccgctacga ccttggcgac gccgtgaccg
      841 tgcatccgga ccgctgtgcc tgcggcagcc atttcccgtc cgtgtcggtc gaaggccgtt
      901 gcgacgacac attgcgcttg cccggtcggc gtggcggcga agtcatgctg gttccgctcg
      961 cgctggaaac ggtgctggag gaaggtgccg gcgcccatga attccaggtc gtgcagcgcg
     1021 ccgatggcgc cctcacgatt catctcgggg cgcaggacca acgccgtcgc ggacccgtcc
     1081 gcgcggcact acgccggtac ttcgagacat gcgacgtcgg cccggtggaa attctgatct
     1141 cacgccgcgg cccgacgcgc gatccggcca gcggcaagct gcgtcgggtc atctgtcggt
     1201 tggcgcactg agggatttcg ccactgtctg cgggttgggc acaatccgca gcatgtcctt
     1261 gcgcgctccc attgcctggt tccgtcgtca ccccgtgctg tggctcttcg gcgcggctct
     1321 gctgctggtc gggctgggcg ccggctgttc gacgttcggt tactacgcgc aatcgatcca
     1381 gggccacatg gccgtgatgg aagcggcgcg gccgatcccc gacgtcctcg acgatccgaa
     1441 cgccgccgtg ccactcaagc aacggctcga gcgcgtgcgg ctgatccgcg cctttgcgtc
     1501 gtccgacctg ggactccccg acaacggcag ctacacgcgt tacgccgacc tgaaacgtcc
     1561 gtacgtggtg tggaacgttt ttgccgcgcc ggaactgtcg ctgcaactca agcagtggtg
     1621 ctacccggtc gtgggctgcg ccggctatcg gggctacttc gaacaggcgg cggccgacgc
     1681 ggcagcggcc gaactgcgtt ccactggcta cgaagtcaac gtcgccggag tgccggccta
     1741 ttcaacgctg ggatggttct ccgatccgct gctcaatacc ttcatcggcg gcagcgaggg
     1801 ccaggtggcc gggctggtct ttcatgaact ggcgcaccag gtcgtattcg tcggcggcga
     1861 cacgaccttc aacgagtcct ttgcgacagc aatcgagcgc tatggagtga agcgctggct
     1921 ggatgcgcgg ggcgacgacg ccacgcgccg ggcttacgcc gagttcgcgc tgcgccgctc
     1981 gcagtttctg gagcttctgc tgaagtatcg caagctgttg caggagaatt actccagcgg
     2041 cgtcccggac gaggtgaaac gcgcgcgcaa gcagcacctg ctttcggaat tgaagcagga
     2101 gtacgcgcgg ctgaaggaaa gctggggcgg ctttgccggc tacgaccgct actttgctca
     2161 ggatctgacc aatgcccatc tggctgcggt cgcggcctac aacgatctcg tccccgcatt
     2221 cgatgcgctg ctggtgcaat cgaaggggaa catgccggcg ttttattccg aagtgaagcg
     2281 catcgcagcg ttgcccaagg ctgaacgcga tgccgcactg acccggctgc agacgtccac
     2341 tcgctagcga accgcgagct cgcggaagcg agcggctatg atctgatcaa ggttgccgct
     2401 tggcttgccg cccgtcgggc ggaacgaccc aatccaataa gacgccgcgt gcgacgtgcg
     2461 aaacagggag acgtaggatg gaccggttct ggctgaagaa ttatccgaag ggcgttccgg
     2521 ccgatatcga ttatcaccag tacaagtcgc tggtgcagtt gctggaagag gccttcaaga
     2581 aatacagcgg gcagccggcc tacagttgca tgggcaaggc cctgaccttc ggcgacctcg
     2641 acatccagtc ggcggcgatt ggtgcctggt tgcagagccg ggggttcacc aagggcaagc
     2701 gcgtggccat catgatgccg aacgtgctgc agtacccggt ggtgctggcc ggtgtgctgc
     2761 gtgccggctg cacggtcgtc aatgtcaatc cgctgtacac gccacgggag ctcgagcacc
     2821 agctcaagga ctccggcgcc gaagcgatct tcattctcga gaacttcgcg acgacgttgc
     2881 agcaggtggc cgacaaggtc ccgaccaaga cggtcgtcgt ctgcgcaatg ggcgacatgc
     2941 tcggcttcgc caagggcctg ctggtcaatt tcatcgtgcg caacgtcaag aagatggtgc
     3001 ccgagtacac gcttgccaac gcgttgcgct ggaacgaggt gcttgccgac ggactgcgca
     3061 tgtcgctgaa gccggtcgac gtgacgctga acgacacggc gttcctgcag tacaccggcg
     3121 gtacgaccgg cgtgagcaag gg
//
LOCUS       DATIRT010000447         2256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1354162, whole genome shotgun sequence.
ACCESSION   DATIRT010000447 DATIRT010000000
VERSION     DATIRT010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354162"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VLN25_10115"
     CDS             complement(<1..472)
                     /locus_tag="VLN25_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204443.1"
                     /note="SAM-dependent;catalyzes the methylation of cytosine
                     at position 1962 of the 23S rRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HSQ10950.1"
                     /translation="MHRIVLKPGKEKSLLRRHPWVYAGAVARVEGDPASGAEVEIRGA
                     DGRFLAWAAYSPESAIRARVWSFVEAVPVSAELLAQRVQAAVERRAMMAADTNALRVV
                     FGEADGLPGLIVDRYADQLVTQFQSAGVEAWRDTLIEALLQATGCRDVFDRSDGA"
     gene            complement(526..1416)
                     /gene="xerC"
                     /locus_tag="VLN25_10120"
     CDS             complement(526..1416)
                     /gene="xerC"
                     /locus_tag="VLN25_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HSQ10951.1"
                     /translation="MRTQRRASGHTLAAYRRDLAKLVELAAGRGPAHLSSHDIRGFIA
                     RLHGAGLSGASLARALSSWRSYFNWLTHRGALDSNPATGVRPPRREKRLPKALTADQA
                     VTLAAYAGDDSWLQARDHALVELMYSSGLRLSELVSLDWRHFEADGDARRSASWIELA
                     NGEATVLGKGSKTRTVPIGAPACTALARWLERRAEVKGCDARALFVSARGGRLTGRSV
                     QSRLGQLARRLGLGIHVHPHVLRHSFASHLLQSSGDLRAVQELLGHANISTTQVYTRL
                     DWQHLAKVYDAAHPRAKRRS"
     gene            complement(1509..2165)
                     /locus_tag="VLN25_10125"
     CDS             complement(1509..2165)
                     /locus_tag="VLN25_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HSQ10952.1"
                     /translation="MQVQDIANYLASHPDFFDQHPELLAGMQLPHPHNGQAISLVERQ
                     SMMLRERIKSLESRVAEMVRHAQENDAISSRLVAWTRALLIEDEPAALPQTLVSAMKE
                     VFSVPHCAVRLWAVKPPYATLVCGRPVSADTITLANSMRVPFCGSNVGFEAATWFDDG
                     ASIQSLAMLPLRVGADPATFGLLVLGSADKDRFHITMGTAFLERIAELASAALARLRA
                     "
BASE COUNT          377 a          782 c          750 g          347 t
ORIGIN      
        1 tggcgccatc gctgcgatcg aagacatcgc ggcagcccgt tgcctgcagc aatgcctcga
       61 tcagcgtgtc gcgccaggcc tcgacgcccg cagactggaa ctgcgtgacg agctgatccg
      121 cgtaacgatc gacgatgagt ccgggcaagc cgtcggcctc gccgaacact acgcgcaggg
      181 cattggtgtc tgccgccatc atcgcgcgtc gctcaaccgc ggcctgtacg cgctgcgcca
      241 gcagctccgc gctgaccggc accgcctcga caaagctcca gacgcgcgca cggatcgcag
      301 actccggtga gtaggctgcc caggcgagaa agcggccgtc ggcaccccgg atctccacct
      361 cggcgccgct ggccgggtca ccctccacgc gcgccacagc accggcgtac acccacggat
      421 gtcggcgcag cagtgacttt tccttgcccg gcttcaggac gatgcggtgc acggtccggc
      481 gcgcgctctg agcaggtgcg gcgtcgcggg ggcggtccga cgcgctcatg acctgcgctt
      541 ggcgcgcggg tgggccgcgt cgtaaacctt ggcgaggtgc tgccagtcca gccgcgtgta
      601 cacctgtgtc gtcgagatgt tggcgtgtcc gagcaactcc tgcacggcgc gcaggtcgcc
      661 actcgactgc agcagatggg aagcgaacga gtgacgcagg acgtgcgggt gcacatggat
      721 acccaggccg agacggcgcg ccagttgccc cagccgcgac tggacgctgc gccccgtcag
      781 gcgaccaccg cgcgcggaga cgaagagcgc tcgcgcatcg cagcccttga cctcggcgcg
      841 acgctcgagc cagcgggcga gcgccgtgca ggccggcgcg ccgatgggca ccgtacgtgt
      901 cttgctgcct ttgccgagca ccgtcgcctc gccgttcgcg agttcgatcc agctcgcgga
      961 gcggcgcgcg tcgccgtcgg cttcaaagtg gcgccagtcg agcgacacca gttccgacag
     1021 gcgcaggccg ctggagtaca tgagttcaac cagcgcgtga tcgcgcgcct gtagccagga
     1081 gtcatctcct gcataggcgg ccagcgtcac cgcctggtcg gcagtcaggg ccttgggcag
     1141 ccgcttctcg cgtctcgggg gccggacacc ggtcgccgga ttggagtcca gcgcgccgcg
     1201 atgcgtcagc cagttgaagt aggagcgcca cgacgacagg gcgcgcgcga gcgatgcacc
     1261 cgacagtccg gcgccgtgca gtcgtgcaat gaagccgcga atgtcgtggg acgatagatg
     1321 cgccgggcca cggccggccg cgagttcgac aagcttggcc aggtcacgcc gataggccgc
     1381 tagcgtgtgc cccgaagcac gccgttgcgt ccgcatttcg tcgaggaagc gctcgatcga
     1441 cggcggctcg accacctcag ttcccgtcag gccgggcgca accggggatc gcgcgtgagc
     1501 gcttcggatc aggcacgaag tcgcgccagt gccgcgctcg ccagctcggc gatgcgctcg
     1561 agaaacgccg tacccatcgt gatgtgaaag cgatccttgt ccgccgatcc gagcaccagc
     1621 aggccgaagg tcgccgggtc ggcgccaacc cggagcggca gcatcgcgag gctctggatc
     1681 gacgcaccgt cgtcgaacca cgtggccgcc tcaaaaccga cgttcgatcc gcaaaacggc
     1741 acgcgcatgc tgttggccag ggtgatcgtg tcggccgata ccggccgccc acagacgagc
     1801 gtcgcgtaag gcggcttgac ggcccagagc cttacggcgc aatgcggaac gctgaagacc
     1861 tccttcatcg cgctcaccag cgtctgcggc agcgcggccg gctcgtcctc gatcagcaag
     1921 gctcgcgtcc acgcgaccag ccgcgacgag atcgcatcgt tttcctgtgc gtgacgcacc
     1981 atctccgcga cgcgcgactc aagcgacttg atgcgttcgc gcagcatcat cgactgtcgc
     2041 tccaccagag agatcgcctg gccgttgtgc gggtgcggca gttgcatgcc ggcgagcagc
     2101 tccggatgct gatcaaaaaa atcgggatgg cttgcgaggt aattcgcgat gtcctgaact
     2161 tgcatgggtc tctcttcgct tctcttctac agatcgatct caccttcgaa aacggtcgac
     2221 gccgggccgg tcatccagac cggcccgccg gtccac
//
LOCUS       DATIRT010000448         3129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_469717, whole genome shotgun sequence.
ACCESSION   DATIRT010000448 DATIRT010000000
VERSION     DATIRT010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469717"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2527
                     /gene="metH"
                     /locus_tag="VLN25_10130"
     CDS             <1..2527
                     /gene="metH"
                     /locus_tag="VLN25_10130"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HSQ10953.1"
                     /translation="QVIDINMDEAMLDSAAAMTRFLNLIASEPDIARVPVMIDSSKWS
                     VIEAGLKCVQGKSIVNSISMKEGEAEFIRQAALARHYGAAVIVMAFDEQGQADTYARK
                     TQICKRSYELLTREVGFPPEDIIFDPNVFAIATGIDEHNNYAVDFIEATHWIRQHLPY
                     AKVSGGVSNVSFSFRGNDHVREAIHTVFLYHAIRAGMTMGIVNAGQLGVYEDIEPELR
                     ERVEDVVLNRRADAGERLVQFAEQVKAGGRKKEEDLAWRAEPVDKRLAHALIHGINNF
                     VVEDTEECRAAIDARGGRPIEVIEGPLMDGMNIVGDLFGAGKMFLPQVVKSARVMKQA
                     VAHLVPFIEAEKAAHVAAGGEARAKGKVVIATVKGDVHDIGKNIVSVVLQCNNFEVVN
                     MGVMVPAEKILATAREEDADMIGLSGLITPSLEEMQHVAREMQRQGFTMPLLIGGATT
                     SRVHTAVKIAPHYTNGPVVYVSDASRSVSVCQSLVSDEAAARYIADVKKEYERVREQH
                     AGKKGPELIELATARANATRVNWTGYRAPKPKFLGRRVFKNFDMAMIAKYVDWGPFFQ
                     TWDLHGPFPQILADDVVGEHARQVFAEGEKMLERIVAERWLTANAAIAFYPANAVGDD
                     IEVYTDDTRREVLFTWHGLRQQTRKPVLDGVPNPNQALADFVAPKDSGIADYIGLFAV
                     TSGIGIEKRESQFQAKHDDYGAIMLKAIADRLAEAFAELMHERVRRDLWGYASGEALT
                     SEELIKERYRGIRPAPGYPACPEHSVKRAMFDVLNAADIGMTLTENYAMHPASAVSGF
                     YLAHPQAKYFNVGRIGRDQLEDLAARKRETVSELERWLAPNLG"
     gene            2619..3065
                     /locus_tag="VLN25_10135"
     CDS             2619..3065
                     /locus_tag="VLN25_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10954.1"
                     /translation="MNPRACLVAAMLIAVSLPVLLASCARSDPEKELRTTISAMAQAI
                     EQHQVGDALDPVADDFTRESGAFGKQDARRLLAGAMLRNERIQLATTVTDVRIEGERA
                     FVKLRVIATGGAGLLPERGQTWEFDTVWMREQGRWKVFNAEWREGL"
BASE COUNT          597 a          983 c         1049 g          500 t
ORIGIN      
        1 gcaggtcatc gacatcaaca tggacgaggc gatgctcgac tcggccgccg cgatgacgcg
       61 cttcctgaac ctgatcgcca gcgagccgga tatcgcgcgc gtgccggtga tgatcgacag
      121 ctccaagtgg agcgtcatcg aagcgggcct gaagtgcgtc cagggcaaga gcatcgtcaa
      181 ctcgatctcg atgaaggaag gcgaggctga attcatccgc caggcggccc tggcgcgaca
      241 ctacggcgcg gcggtcatcg tgatggcctt cgacgaacag ggccaggcgg acacctacgc
      301 gcgcaagacc cagatctgca agcgctcgta cgaactgctg acgcgcgagg tcggtttccc
      361 gccggaagac atcatcttcg atcccaacgt cttcgcgatc gctacgggca tcgacgagca
      421 caacaactac gcggtcgact tcatcgaggc cacgcactgg atccggcagc acctgccgta
      481 cgcgaaagtg agcggcggcg tcagcaacgt cagtttcagc ttccgtggga acgaccacgt
      541 gcgcgaggcg atccacacgg tgttcctgta tcacgcgatc cgtgccggca tgacgatggg
      601 catcgtcaac gccggccagc tcggtgtgta cgaggacatc gaaccggagt tgcgcgaacg
      661 cgtggaggac gtcgtgctca accgccgcgc ggatgccggc gaacggctgg tgcagttcgc
      721 cgagcaggtg aaggccggtg gccggaagaa ggaagaagac cttgcctggc gcgccgagcc
      781 ggtcgacaag cgcctcgccc atgcgctgat ccacggcatc aacaactttg tcgtcgagga
      841 caccgaagag tgccgcgccg ccatcgacgc gcgcggtggc cgcccgatcg aagtgatcga
      901 gggcccgttg atggacggca tgaacatcgt cggggatctg ttcggcgcag gcaaaatgtt
      961 cctgccacag gtggtgaagt ctgcgcgtgt gatgaagcag gcagtcgcgc acctggtgcc
     1021 gttcatcgaa gccgagaagg ccgcgcatgt cgccgccggc ggcgaggcgc gtgccaaggg
     1081 caaggtcgtg atcgccaccg tcaagggcga cgtgcacgac atcggcaaga acatcgtgtc
     1141 ggtcgtgctc caatgcaaca acttcgaagt cgtcaacatg ggcgtcatgg tgccggccga
     1201 gaagatcctc gccactgcgc gcgaggagga cgccgacatg atcggcctgt ccgggctgat
     1261 cacgccgtcc ctggaggaaa tgcagcacgt cgcgcgtgag atgcagcgac agggcttcac
     1321 gatgccgctg ctgatcggag gagccacgac cagccgcgtg cacacggcgg tgaagatcgc
     1381 gccgcactac acgaacggcc ccgtggtcta cgtgtcggac gcgagccgct cggtcagcgt
     1441 atgccagagc ctggtcagtg acgaggccgc ggcccgctat atcgccgacg tcaagaagga
     1501 atacgaacgc gtgcgtgaac agcatgcggg caagaaaggc ccggagctga tcgaactcgc
     1561 caccgcccgc gcgaatgcga cgcgcgtcaa ctggaccggc taccgtgcgc ctaagccgaa
     1621 gttcctcggt cgccgtgtgt tcaaaaattt cgacatggcg atgattgcga agtacgtcga
     1681 ctggggcccg ttcttccaga cctgggatct gcacggaccc tttccgcaga tcctggccga
     1741 cgatgtcgtc ggcgagcatg cgcgccaggt cttcgccgag ggcgagaaga tgctcgagcg
     1801 catcgtcgcc gagcgctggc tcaccgcgaa cgccgcgatc gcgttctatc cggccaatgc
     1861 ggtcggcgac gacatcgagg tctacaccga cgacacgcgg cgcgaagtac ttttcacctg
     1921 gcacggcctg cgccagcaga cccgaaagcc ggtgctcgac ggtgtgccga atccgaacca
     1981 ggcgctggcg gatttcgtgg cgccgaagga ctcgggcatc gccgactaca tcggtctctt
     2041 cgccgtgacg agcggaatcg gcatcgagaa gcgcgagtcc cagttccagg cgaagcacga
     2101 tgactacggc gcgatcatgc tgaaggcgat agcggaccgt ctggccgaag cctttgccga
     2161 actgatgcac gaacgcgtgc gccgggacct ctgggggtat gcctccggcg aggcgctgac
     2221 cagcgaggaa ctcatcaagg agcgctatcg cggcatccgg cccgcgccgg gatatccggc
     2281 gtgcccggag cattcggtga agcgggcgat gttcgatgtc ctcaatgccg ccgacatcgg
     2341 gatgacgctc accgagaact acgcgatgca tccggcctcc gcggtcagcg gcttctatct
     2401 ggcccatccg caggcgaagt acttcaatgt cggccgcatc ggacgcgacc agctcgagga
     2461 cctggcagcg cgcaagcgcg agaccgtcag cgaacttgaa cgctggctcg cgccgaatct
     2521 cggctgagtt tgcagcccgg cctgcgctgc cgaaaagtga cagtggcgtg tccagtcctt
     2581 aacctgttgc ccgccatgtc actgctgcga tgctggccat gaacccccgc gcttgcctcg
     2641 tcgcggcgat gttgatcgcc gtatcgctac cggtgctcct ggcgtcgtgt gcccgctcgg
     2701 atccggaaaa ggagttgcgt acgaccatct cggccatggc gcaggcgatc gagcagcatc
     2761 aggtcggcga cgcgctcgac ccggtcgccg acgacttcac gcgcgagtcc ggtgcctttg
     2821 gcaagcagga tgcacgacgc ctgcttgccg gcgcgatgct gcgcaacgag cgcatccagc
     2881 tggctaccac ggtgaccgat gtccgcatcg aaggcgagcg cgcgttcgtc aagctgcggg
     2941 tgatcgcgac cggcggtgcg gggctgctgc cggagcgcgg gcagacctgg gaattcgaca
     3001 cggtgtggat gcgcgagcag ggacgctgga aggtcttcaa tgccgagtgg cgcgaaggac
     3061 tgtagcgatc gtcgcggcca cgggtcgtgc actgcagatt gcgtgagagt gcgcgcagtg
     3121 ccatcgtgc
//
LOCUS       DATIRT010000449         2342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1059744, whole genome shotgun sequence.
ACCESSION   DATIRT010000449 DATIRT010000000
VERSION     DATIRT010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059744"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="VLN25_10140"
     CDS             <1..775
                     /locus_tag="VLN25_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSQ10955.1"
                     /translation="VSGAFEHNINMQAKGQPMRAFVLQGRAPQIVLAVSNKTMPNFKS
                     VADLKGKKIGVTAPGSSTNIMTNFVLAKNGLKATDVSYVGVGASQGAVAALRSGQIDA
                     ISNLDPVITMLTRNNDIKIISDTRDVAEADKVFGGPMPAATLYSPVAFIEKNPNTVQA
                     LTNAIVRANKWIQQAGPSDIVKAVPESFLLGDRAVYIDAFLKAKPALSPDGMIPEQGP
                     ATALRALTSIDPTLANAKIDLKSVYTNDFVKKANAKYPK"
     gene            831..1748
                     /locus_tag="VLN25_10145"
     CDS             831..1748
                     /locus_tag="VLN25_10145"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSQ10956.1"
                     /translation="MSGPTREVRLTAAVSLQGITCTFVSRDDRSQRYTAVRDVSLDIA
                     PGEFVSVVGPTGCGKSTLLNVAAGLLEPSSGTVHVFGEPLAGINRRAGYMFQSEALMP
                     WRTARDNVLAGLQFRGMTESDASAVADDWLQRVGLGGFGDRYPHQLSGGMRKRTSLAQ
                     TLVLDPDIILMDETFSALDVQTRQLMENELLELWARGGDARRAAPSKSPAPWGGGREA
                     AGGATNRKAVLFITHDLDEAIAMSDRVVVLSAGPASHPIGEFVIDLARPRDVAEVRVT
                     PRFIDLHKAIWNVMREEVLKGYAQQKQAA"
     gene            1759..>2342
                     /locus_tag="VLN25_10150"
     CDS             1759..>2342
                     /locus_tag="VLN25_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSQ10957.1"
                     /translation="MWNAIRPNTKNLRFWQVGVLVVLFLLWHVLTKPGLVPPLFFDGE
                     NDAKFFFGEPVKILERIWAWFVVDRDIYQHLWVTLLETVLAFLIGTVSGLVMGMWLAL
                     SPVAAAILDPYIKAMNSMPRVILAPIFAVWFGLGIASKVALGFTLVFFIVFFNVYQGV
                     KEVSPTVLASARMLGASRRQLLRHVYLPSATSWV"
BASE COUNT          410 a          747 c          765 g          420 t
ORIGIN      
        1 cgtgtcgggc gcgtttgaac acaacatcaa catgcaggcc aagggccagc cgatgcgtgc
       61 cttcgtgctg cagggaaggg cgccgcagat cgtgctggcc gtcagcaaca agacgatgcc
      121 gaacttcaag tcggtggcgg acctgaaggg caagaagatc ggtgtgactg cgccgggctc
      181 ctcgaccaac atcatgacca acttcgtgct ggcaaagaac ggcctgaagg cgacggacgt
      241 gtcctatgtg ggcgtgggcg catcccaggg ggcggtagct gcgctccggt ccgggcagat
      301 cgacgccatc tcaaaccttg atccggtcat cacgatgctg acccgcaaca acgacatcaa
      361 gatcatttcc gatacgcgcg acgtggccga ggccgacaag gtcttcggtg gaccgatgcc
      421 ggccgcgacg ctgtactcgc cggttgcgtt catcgagaag aacccgaaca ccgtgcaggc
      481 cctgaccaac gcgatcgtgc gcgccaacaa gtggattcag caggccgggc cgtccgacat
      541 cgtgaaggcg gtccccgaga gctttctgct tggcgatcgc gcggtctata tcgacgcctt
      601 tctcaaggcg aagccggcgc tctcgccgga cggcatgatt cccgaacagg gcccggccac
      661 cgcgctgcgc gcgctgacta gcatcgatcc gacgttagcg aacgccaaga tcgacctcaa
      721 gtccgtctac acgaacgact tcgtgaagaa agccaacgcc aagtacccga agtgaccggc
      781 agcgggacgg gtcgcatggc ggcggaccct taccttttgc cggcgcggca atgtccggcc
      841 caactcgaga ggttcgtttg acagcggcgg tgtcactgca gggcattacc tgcacgtttg
      901 tcagccgtga cgaccgttcg caacgctata cggctgtgcg cgacgtttcg ctcgacattg
      961 cgccaggcga gttcgtcagc gtcgtcggtc ccactggctg cggcaagagc accctgctca
     1021 acgtggcggc cggcctgctc gagcccagca gcggaaccgt gcatgtgttc ggcgaaccgc
     1081 tcgccggcat caaccgccgt gccggctaca tgttccagtc cgaggcgctg atgccgtggc
     1141 gcacggcacg cgacaacgtg ctggccggcc tgcagttccg cggcatgacc gagtcggacg
     1201 cgtcggccgt ggccgacgac tggctgcagc gcgttggcct cggcggtttc ggggatcgtt
     1261 atccgcacca gttgtccggt ggcatgcgca agcgcacgtc gctggcgcag acgctggtgc
     1321 tggatcccga catcattctg atggacgaaa cgttttccgc actcgacgtc cagacccgtc
     1381 agctgatgga aaacgaattg ctcgagctct gggcacgcgg cggcgacgcg cgccgggccg
     1441 ccccaagcaa gtcgccggcc ccctgggggg gcggacgcga agcggccggg ggcgcaacaa
     1501 acaggaaggc ggtgcttttc atcacccacg acctggacga ggcgatcgcg atgagcgacc
     1561 gcgtggttgt gctgtcggcc gggccggcat cgcacccgat cggcgagttc gtgattgacc
     1621 tcgcgcgccc gcgcgatgtg gccgaggtgc gggtgacccc gcgctttatc gacctgcaca
     1681 aggcgatctg gaatgtgatg cgcgaggaag tcctgaaggg ctacgcgcag cagaagcagg
     1741 ccgcgtgagc cgaccctcat gtggaacgcg atccgtccca acacgaagaa cctgcgcttc
     1801 tggcaggtgg gcgtgctggt cgttcttttc ctgctctggc acgtactgac caagcccgga
     1861 ctggtgccgc cgctgttctt cgatggcgag aacgacgcca aatttttctt cggcgagccg
     1921 gtcaagatcc tcgagcgaat ctgggcctgg ttcgtcgtcg accgcgacat ctaccagcac
     1981 ctgtgggtga ccctgctcga gaccgtgctg gcgttcctga tcggcaccgt gtccgggctg
     2041 gtgatgggca tgtggctggc gctgtcgccg gtcgcagccg cgatcctcga tccgtacatc
     2101 aaggcgatga actcgatgcc acgcgtgatc ctggcgccga tctttgcggt gtggttcggg
     2161 ctcggcatcg cctccaaggt cgcgctcggc ttcacattgg tgttcttcat cgtcttcttc
     2221 aacgtctacc agggtgtcaa ggaagtcagc ccgacggtgc tcgcgagcgc gcggatgctc
     2281 ggggcttcgc gccgacagct gttacgccac gtctacctgc cgagcgccac cagctgggtg
     2341 tt
//
LOCUS       DATIRT010000450         9699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_961187, whole genome shotgun sequence.
ACCESSION   DATIRT010000450 DATIRT010000000
VERSION     DATIRT010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9699
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961187"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1545)
                     /locus_tag="VLN25_10155"
     CDS             complement(<1..1545)
                     /locus_tag="VLN25_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSQ10958.1"
                     /translation="MWMTRVSINHPVFATMVMVGLTVLGLFSYARLGVEPMPDVSPPG
                     VQIWVNYPGASPEQVENDLAKPIENAANTVAGVKRILSRSDEGRSLTWVEFRYEVDMD
                     RAIQEVRDKLAQIRATFPRDAKDPVVQRAGGENDQPVASFALIGSGKSSRELTMLAEQ
                     VVQKGFERVKGVGRVDLGGNVTRQIQVRVDPAKLTAFGLSVDTVVQALRTANVSVPVG
                     TIHNETSESIIRVDGRLTSPEQFARIIVARKSGVPILLSQVATVVDAERERQSLSRVN
                     GQPAIGVSVFKAQDANIVEVGRELKYAAETIRKQLPAGMELKFMWAMADFVDQSVGNV
                     KTTIIEGALLTVIIVFLFLGSWRSTVITGLTLPISVIATFIVIYALGFTLNYMTLMAL
                     SLCIGLLIDDAIVVRENIVRHIHMGKDHYTAAREGTDEIGLAVLATTLSIVAVFVPID
                     FMSGIIGKFFYPFGITVAAAVLVSLFVSVTLDPMLSAIWRDPPEGARGLPFVGPILRA
                     FERGIDK"
     gene            complement(1613..2800)
                     /locus_tag="VLN25_10160"
     CDS             complement(1613..2800)
                     /locus_tag="VLN25_10160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSQ10959.1"
                     /translation="MTKRAKLVWVVGLLVLAIAGGGGAVIASRQAKPAQAALKTDDRP
                     PLEFAQADVVTLQSKRLSVELAAPGSVLAVSQATVRSKLSAEIKRVLVREGDKVSVGQ
                     VVAEFDTAQLRAQLAERTALLASAKAQLATTERTRQSNAQLVKQNFISQNAFDTADSA
                     HQAQLATVAAMQAQLEQTQILMSDSVVRAPISGIVAKRHVQPGEKLAFDAPLMAIVDL
                     SELEVQAQVPVSDVPQIRKGMPAQVDIEGMSGRRFTGRVERINPSTEPGTRTVNIYVS
                     LPNEESLLKAGMFARVLLTTMPDTEAASLPMSAVRIDGQQSYVWLIADGKLIRRNIAT
                     GRRDERAQLVEITSGLAAADVVLATKFDNLKDGLAAKVVGLPGGSKVADRRATTSGSP
                     ASN"
     gene            complement(3029..4480)
                     /gene="ntrC"
                     /locus_tag="VLN25_10165"
     CDS             complement(3029..4480)
                     /gene="ntrC"
                     /locus_tag="VLN25_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HSQ10960.1"
                     /translation="MKPIWIVDDDRSIRWVLEKALARENLPCKAFGVAQDVLNELEHD
                     SPQVLISDIRMPGMSGIELLRAVKRRHPALPVIVMTAFSDLESAVSAFQGGAFEYLPK
                     PFDVDKAVELIRRAVDESVREASTDEALGEVPEILGQAPAMQDVFRAIGRLSQSNVTV
                     LLTGESGSGKEVVARALHKHSPRVAAPFIALNMAAIPRDLLESELFGHERGAFTGAQA
                     MRRGRFEQAEGGTLFLDEIGDMPAELQTRLLRVLSDGFYYRVGGHQPIKADVRVIAAT
                     HQNLEERVRQGLFREDLYHRLNVIRLRLPALRERSEDIPLLTRHFLVKSAQELGVEPK
                     RLSEEATRFISQLPFPGNVRQLENLCHWLTVLAPAQVVKVEDLPLEMRQELMAAPAVR
                     LNPTEAAAVAPVMVASAAAGGDWTSSLIAEVERRLQSGVPDVMDTLARDFEAAVIRTA
                     LRHTHGRRIDAATRLGVGRNTITRKIQELQLED"
     gene            complement(4489..5574)
                     /gene="glnL"
                     /locus_tag="VLN25_10170"
     CDS             complement(4489..5574)
                     /gene="glnL"
                     /locus_tag="VLN25_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HSQ10961.1"
                     /translation="MSAVARFDPVVEAQFDGLDLLSTAVIVLDQAARIVHANQSAELL
                     LSMSRKNLLGQSAERVIGDEMQMQQLVADARANVFGERRQVLELKRLGREPVPVRVTA
                     TAQYGSSTPLVLEISEIEQQLKVDREERQLGLSEANRQLIRNLAHEIKNPLGGVRGAA
                     QLLDAELASPEQREYTRVIIAEADRLQALVDRLLAPHRVARVVAELNIHEVCERVRMV
                     MLAEFPRGLTIERDYDASVPNFHGDREQLIQALLNIVRNATQALESRIDAGDARITLR
                     TRVARQVTIARSRHRLALDLHVTDNGPGVPNDIKDRIFYPLVSGRDDGTGLGLTLAQT
                     FIQQHDGTIEVDSRPGRTDFRILIPLP"
     gene            complement(5689..6195)
                     /locus_tag="VLN25_10175"
     CDS             complement(5689..6195)
                     /locus_tag="VLN25_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HSQ10962.1"
                     /translation="MLPGFALWIGVAMSWSTHAQSSDIFMCVDDQGNKSFQNVGNGKG
                     CKRVDVGPVLSVPATRQPAARGSVQPAVEERASVSPASFPRVDRDTQRTRDSDRRRIL
                     EDELKAEEDRLARLRSEFNNGEPERRGDERNFALYQERIQRLREDIGRVETNVGALRR
                     EIALLRTQ"
     gene            complement(6388..7800)
                     /gene="glnA"
                     /locus_tag="VLN25_10180"
     CDS             complement(6388..7800)
                     /gene="glnA"
                     /locus_tag="VLN25_10180"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HSQ10963.1"
                     /translation="MATAKDVIKMITDNEALFVDLRFTDTKGKEQHVSVPAKAFEEDK
                     FEHGHAFDGSSIAGWKGIEASDMLLMPDPSTARMDPFREENTLIITCDVVEPSTGKGY
                     DRDPRSLARRAEAYLKSTGVGDTAYFGPEPEFFIFDSVTWNVDMSGSAVRIRSEEAPW
                     STGIEYEAGNLAHRPPVKGGYFPVPPTDSLQDIRSEMCLLLEQQGVEVEVHHHEVAAP
                     GQCEIGTKFSTLVKRADWLQIMKYTIWNVAASYGKTATFMPKPIVGDNGSGMHVHQSV
                     WKEGTNLFAGNGYGGLSDFALYYIGGIIKHARSLNAITNPGTNSYKRLVPGFEAPVKL
                     AYSARNRSASIRIPYVSNPKGRRIEVRFPDPTANPYLAFAAMLMAGLDGVQNKIHPGE
                     AADKNLYDLPPEEDAKIPTVCSSLDQALEYLDKDREFLTRGGVFSNDLIDAYIALKMD
                     EVTRFRMTTHPVEFDMYYGS"
     gene            complement(8039..9292)
                     /gene="hemW"
                     /locus_tag="VLN25_10185"
     CDS             complement(8039..9292)
                     /gene="hemW"
                     /locus_tag="VLN25_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HSQ10964.1"
                     /translation="MDVIPIDVALAGPAPTDIAAYLRPGTLSLPALPPLSLYVHYPWC
                     VRKCPYCDFNSHEPEGGPGTIPEADYLTALRSDLEAALPLIWGRRIVSIFIGGGTPSL
                     LSARGLDTLLSDVRALLPLEGDCEVTLEANPGTVEAGRFADYRRSGVNRLSIGVQSFD
                     DSRLQALGRIHDREQALRAIDTARRVFDNFNIDLMYGLPSQDLAGAQADVEQALALAP
                     PHLSLYQLTLEPNTVFAKHPPPLPDEDTAASMQAWIEGRTAAAGYRHYEVSAYAQPAR
                     ECRHNLNYWGFGDYLGVGPGAHSKISFPHRIIRQVRYRGPRTYLERAGRREFLAESSE
                     VARDDLPFEFMLNAMRLLDGVPSRWFTERTGLPPTAIEKALLEAERRGLVATDHAHWA
                     PTPLGARFLSDLQALFLPRSLRDPN"
     gene            complement(9312..>9699)
                     /locus_tag="VLN25_10190"
     CDS             complement(9312..>9699)
                     /locus_tag="VLN25_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HSQ10965.1"
                     /translation="DDSGLCVASLQGAPGVRSARFAGPTADDAANNAELLRRLQEHDD
                     RRAHYTCVLVAVASANDPEPLVADARWQGEILHSPRGSGGFGYDPLFLVPECGCTAAE
                     LSAEEKNRISHRGRAMRELVRQLAAW"
BASE COUNT         1687 a         3190 c         3062 g         1760 t
ORIGIN      
        1 cttgtcgatg ccacgctcga aggcgcgcag gatcggcccc acgaacggca agccgcgcgc
       61 gccctccggc gggtcacgcc agatggccga cagcatcggg tcgagcgtga cgctcacgaa
      121 cagcgacacc aggaccgccg ccgcgacggt aatgccgaac gggtagaaga acttgccgat
      181 gatgccgctc atgaagtcga tcggcacgaa caccgcgacg atcgaaagcg tggtcgccaa
      241 cacggccagg ccgatctcgt cggtcccttc gcgcgcggcc gtgtagtgat ccttgcccat
      301 gtggatgtgg cgcacgatgt tctcgcgcac cacgatcgcg tcatcgatca gcaggcctat
      361 gcacagcgac agcgccatca gcgtcatgta gttcagcgtg aatccgagtg cgtagatgac
      421 gatgaacgtg gcgatcacgg agatcggcaa cgtcagcccg gtgatgacgg tgctgcgcca
      481 ggatcccagg aacaggaaaa cgatgatgac cgtcagcagc gcaccttcga tgatcgtcgt
      541 cttcacgttg ccgaccgact ggtcgacgaa gtcggccatc gcccacatga acttcagctc
      601 catgcccgcg ggaagctgct tgcggatggt ctccgcggcg tacttcagct cgcgtcccac
      661 ctcgacgatg ttggcgtcct gcgccttgaa cacggataca ccgatcgccg gctggccgtt
      721 gacgcgtgaa agcgactgcc gctcgcgctc ggcgtcaacc accgtcgcga cctgtgagag
      781 caggatcgga acaccgctct tgcgggcgac gatgatgcgt gcgaactgct cgggcgacgt
      841 cagccgtccg tcgacgcgga tgatcgattc cgatgtctcg ttgtggatgg tgccgaccgg
      901 caccgacacg ttggccgtgc gcagcgcctg gaccaccgtg tcgaccgaca ggccgaatgc
      961 cgtcagcttg gccggatcga cacgcacctg gatctggcgc gtgacgttgc cacccagatc
     1021 gacgcggccg acgcccttca cccgctcgaa gcccttctgc acgacctgtt cggcaagcat
     1081 cgtcagctcg cgcgaggact tgccgctgcc gatcagggca aaggaggcga ccggctggtc
     1141 gttctcgccc ccggcacgct gaaccaccgg atccttcgca tcacgcggga aggtcgcgcg
     1201 gatctgcgcc agcttgtcgc gcacctcctg gatcgcgcgg tccatgtcca cttcgtagcg
     1261 gaactccacc cacgtcagcg atcggccttc gtcagaacgc gacaggatgc gcttgacgcc
     1321 cgcgaccgtg ttcgcggcgt tttcgatcgg cttggcgaga tcgttttcaa cctgctcggg
     1381 cgaggctccg ggatagttga cccagatttg aacgcctggc gggctgacgt cgggcatcgg
     1441 ctcgacacca aggcgcgcgt aggaaaacag gcccagcacg gtcaggccca ccatcaccat
     1501 ggtcgcgaag accggatggt tgattgaaac tcgcgtcatc cacatgtgtg cagtcctccc
     1561 gaacgcgggg cgaggcggcc tggcaggcgc ctcactccgt ctgcgcccgt gttcagttcg
     1621 aagccggtga cccgctcgtc gtcgcgcgtc ggtcggcgac cttcgatccg cccggcaggc
     1681 cgacgacttt cgcggccagt ccgtccttca gattgtcgaa cttggtcgcg aggacaacgt
     1741 ccgccgccgc cagccctgac gtgatttcca ccagttgagc gcgttcgtcc cgccggcccg
     1801 tcgcgatgtt gcggcgtatc agcttgccat cggcaatcag ccagacgtag ctctgctggc
     1861 cgtcgatgcg caccgcggac atcggcagcg acgctgcctc ggtatccggc atcgtcgtga
     1921 gcaatacgcg ggcaaacatg ccggccttca gcagcgactc ctcattggga agcgagacgt
     1981 agatgttgac ggtgcgcgtt cccggttcgg tactgggatt gatgcgttcg acgcgtccgg
     2041 taaagcgacg accgctcatg ccttcgatat ccacctgcgc cggcatccct ttcctgatct
     2101 gcggaacgtc ggacaccggc acctgggcct ggacttcgag ttccgacaga tcgacgatgg
     2161 ccatcagcgg cgcatcgaac gcgagcttct cgcccggctg cacgtggcgc ttggcaacga
     2221 tgccggaaat gggcgcgcgc accaccgaat cgctcatcag gatctgcgtc tgctcgagtt
     2281 gcgcctgcat tgctgccacc gtggccagct gtgcctgatg agcagaatcc gccgtatcga
     2341 acgcgttctg ggaaatgaag ttctgcttga ccagctgcgc attggattgg cgcgtgcgct
     2401 cggtcgtggc gagctgggcc ttagcagacg ccagcagggc cgtgcgctcg gcgagctggg
     2461 cacgaagctg cgccgtgtcg aattcggcca ccacctggcc gacgctgacc ttgtcgccct
     2521 cgcggaccag cactcgcttg atctcagctg acagcttcga tctcactgtg gcctgtgaca
     2581 cggccagcac actgccggga gctgcgagct ccaccgaaag gcgctttgac tgcagggtta
     2641 ctacatcggc ctgtgcgaac tccagtggcg ggcggtcatc cgtcttcaag gcggcctgcg
     2701 ccggtttggc ctgtcgcgag gcgatgacgg cacccccgcc ccccgctatg gccagcacca
     2761 gcagcccgac cacccacaca agctttgcgc gtttcgtcat cgattccccc aggacggccg
     2821 cggcaccggg ttggcgccag aagtgtgcgc aggttcgtga cccgcgggcc gtgtcttatc
     2881 gtgggagcgg attatccgca accactccgg attgtcgccg ttggtgcgac gaattgcagg
     2941 cagccgcgac gaaacgcagt aaaagtggac ggagggccgg cagccctcag gttcggaatt
     3001 gggcctcgaa ccgccctgcg tcagcccctc agtcttccag ctgcagttcc tggatcttgc
     3061 gggtaatcgt gttgcgtccg acgccgagac gcgtggcggc gtcgatgcgg cgtccgtggg
     3121 tgtgccgaag tgccgtgcgt atgaccgccg cctcgaaatc gcgcgccagg gtatccatga
     3181 catcgggaac cccggattgc agccggcgtt cgacctcggc aatcagcgaa gacgtccagt
     3241 cgccccctgc ggcggccgat gcaaccatca ccggagccac tgccgcggcc tccgtcgggt
     3301 tcaatctcac ggccggcgcc gccatcagtt cctgccgcat ttcaagcgga aggtcttcga
     3361 ccttgaccac ctgggccggt gcgagcacgg tcagccagtg acacaggttt tcaagctgtc
     3421 gtacgttgcc gggaaacggc aactggctga tgaagcgcgt cgcttcctcg gacagccgct
     3481 tcggctcgac ccccagctcc tgcgcgctct tgacgaggaa gtgccgggtc agcagcggaa
     3541 tgtcttcgct gcgttcgcgc agcgccggca ggcgtagccg gatgacgttc agccgatggt
     3601 agagatcctc gcgaaacagg ccctgccgaa cacgctcttc gagattctga tgagttgctg
     3661 cgatcacgcg cacgtcggcc ttgatcggct ggtgtccgcc gacgcgatag taaaagccgt
     3721 cggacagcac gcgcagcagg cgcgtctgca gttccgccgg catgtcgccg atctcgtcga
     3781 ggaaaagcgt cccgccttcg gcctgttcga agcggccccg gcgcatcgcc tgcgcgcccg
     3841 tgaatgcgcc acgttcatgt ccgaacaatt cgctctccag caggtcgcgc ggaatcgccg
     3901 ccatgttgag cgcaatgaag ggggccgcca ctcgcgggct gtgcttgtgc agggcacgcg
     3961 cgacgacttc cttgccgctc ccggactcgc cggtgagcag cacggtcacg ttcgactggc
     4021 tgagacggcc gatggcccgg aacacatcct gcatcgctgg cgcctgccct aggatctcgg
     4081 gtacttcgcc aagggcttcg tcggtgctcg cttcgcgcac gctctcgtcc accgcacgtc
     4141 ggatcagttc gaccgccttg tcgacatcga acggcttggg caggtactcg aatgcgccgc
     4201 cctggaatgc cgacaccgcg ctttcgagat ccgagaacgc ggtcatcacg atcaccggca
     4261 acgccgggtg ccgacgcttg accgcccgca acagctcgat gccgctcatc ccgggcatgc
     4321 ggatgtcgga aatcagcacc tgcgggctgt cgtgctcgag ttcgttcagc acgtcctgcg
     4381 ccacgccgaa agccttgcac ggcaggtttt cgcgcgccag cgccttttcc agcacccagc
     4441 gtatcgagcg atcgtcgtcg acgatccaga ttggtttcat ggcagcgctc atggcagcgg
     4501 aatcaggatg cggaaatcag ttcggcccgg tcggctgtcg acctcaatgg tgccgtcgtg
     4561 ctgttggatg aacgtctgtg caagcgtgag ccccaggccg gtgccgtcgt cgcgtcccga
     4621 caccagcgga tagaagatgc ggtccttgat gtcattcggc acgcccggcc cgttgtcggt
     4681 cacatgcaag tccagtgcca ggcgatggcg ggatcgagcg atcgtcacct ggcgcgccac
     4741 ccgagtgcgc aacgtgatgc gtgcatctcc cgcatcgatg cgtgattcca gcgcctgggt
     4801 cgcgttgcga acgatgttaa gcagcgcctg aatcagctgc tcccggtcgc cgtggaagtt
     4861 cggcacgctg gcgtcgtagt cacgctcgat cgtgaggcca cgcggaaact ccgccagcat
     4921 caccattcgt acgcgctcgc agacttcgtg gatgttgagt tcggcgacga cccgcgcgac
     4981 acgatgcggc gcaagcagtc ggtcgaccag cgcctgcaga cggtcggcct cggcaatgat
     5041 cacgcgcgtg tattcgcgct gctccggcga ggcgagctcg gcgtccagca actgggcagc
     5101 gccgcgtaca ccgccaagcg ggttcttgat ctcgtgcgca aggttgcgaa tgagctgccg
     5161 gttcgcttcc gacagtccga gttgccgctc ctcgcggtcg acctttaact gctgctcgat
     5221 ttcggagatt tccagcacca gcggggtgct ggacccgtac tgcgccgtgg ccgtcacccg
     5281 gacaggcacc ggctcgcgtc cgagtcgctt gagttccagg acctggcggc gctcgccgaa
     5341 gacattggcg cgcgcgtcgg cgaccagttg ctgcatctgc atctcgtcgc cgatcacacg
     5401 ctcggccgac tgcccgagca ggttcttgcg cgacatgctc agcagaagtt cagccgactg
     5461 gttggcgtgc acgatgcgcg ccgcctgatc gagcacgatc accgcggtcg agaggaggtc
     5521 gagtccatcg aactgcgctt caacgacagg atcgaaccgg gcgaccgcgc tcatcgggtc
     5581 aacccgcgcg aaccttcgaa aacattggcg aggcgaaagg tccgacggac cgacgcaggc
     5641 ccgcgcgccc ggccgctgca aacgcgcgcc ggttgggtcc tgccgtaact actgagtgcg
     5701 gagcagtgcg atttcgcggc gcagggcgcc gacgttggtc tcgactcgcc cgatgtcctc
     5761 gcgcagccgc tggatgcgct cctggtacag cgcgaagttg cgctcgtcac cgcgccgctc
     5821 cggctcgccg ttgttgaact cggaccgcag gcgcgcgagc cggtcttcct cggctttcag
     5881 ttcgtcttcc agtatgcgac gccggtcgct gtcgcgcgtg cgctgtgtgt cgcgatcgac
     5941 ccgcggaaac gacgcaggtg agacagacgc acgctcctcg acggccggct gaaccgaacc
     6001 gcgcgcagcg ggctgtctcg tcgccggaac cgacagcacc ggaccgacat cgactcgctt
     6061 gcagcctttg ccgttgccga cgttctggaa cgacttgttg ccctgatcgt cgacgcacat
     6121 gaagatgtcc gacgactggg catgggtcga ccacgacatg gccactccga tccacagcgc
     6181 aaaacccggc aacattgtct tcactcgcgt gccccttgcg acacagaccg ccggcagtgt
     6241 aaggcagcca ggacgtccgg aagagtgcaa ctacaagcag ttccacggtc agttcccgca
     6301 atttagggag gttgggatca tccgcagccg gcgcaagcaa acaggggcgg tttcccgccc
     6361 ccatttggct ttgacggccg gttcgattta cgacccgtag tacatgtcga actcgaccgg
     6421 gtgcgtcgtc atccggaagc gcgtgacctc gtccatcttc aacgcgatgt aggcgtcgat
     6481 caggtcattg ctgaagacac cgccgcgtgt caggaactcg cggtccttgt cgaggtactc
     6541 gagcgcctgg tcgagcgacg agcacacggt cgggatcttc gcgtcctctt ccgggggcag
     6601 gtcgtacagg ttcttgtcgg cggcctcgcc cggatggatc ttgttctgaa caccgtcgag
     6661 accggccatc agcatcgcgg cgaacgcgag gtacggattc gcggtcggat ccgggaagcg
     6721 cacttcgatc cggcgccctt tcgggttgct gacataggga atgcggatcg acgccgagcg
     6781 gttgcgggcg gaatacgcga gcttgaccgg tgcttcgaag cccggcacca gccgcttgta
     6841 actgttggtg cccggattgg tgatcgcgtt cagcgagcgc gcgtgcttaa tgatgccgcc
     6901 gatgtaatac agcgcgaagt ccgagagccc accgtagcca ttgccggcaa acagattcgt
     6961 gccttctttc cacaccgact ggtgtacgtg catgcccgaa ccgttgtcgc caacgatggg
     7021 cttcggcatg aaggtcgccg tcttgccgta gctcgccgcg acgttccaga tcgtgtactt
     7081 catgatctga agccagtcgg cccgcttgac cagcgtcgag aacttcgtac cgatctcaca
     7141 ttggccgggc gcggcgactt cgtggtgatg cacctcgacc tcgacgccct gctgctcgag
     7201 caacaggcac atctctgagc ggatgtcctg caacgaatcg gtgggtggca cggggaagta
     7261 gccacccttg accggtgggc gatgcgcgag gttgcccgct tcgtactcga ttccggtcga
     7321 ccacggggcc tcctcggacc tgatgcggac tgcgctgccc gacatgtcca cattccaggt
     7381 gaccgaatca aaaatgaaga actcgggctc gggaccgaag tacgcggtat cgccgacacc
     7441 ggtcgacttc aggtatgcct cggcgcgtcg ggcgagtgag cgtggatcgc ggtcatagcc
     7501 cttgccggtc gacggctcga cgacatcgca ggtgatgatc agcgtgttct cttcgcggaa
     7561 cgggtccatg cgggccgtcg agggatcggg catcaggagc atgtccgagg cctcgatgcc
     7621 cttccagccg gcgatcgagg aaccgtcgaa cgcgtgtccg tgttcgaact tgtcctcctc
     7681 gaatgccttg gccggcacgg acacatgctg ttccttgccc ttggtgtcgg taaaccgcag
     7741 atcaacgaac agcgcctcgt tgtccgtgat catcttgatg acgtctttgg ccgtggccat
     7801 tctttctcct caatcgctcg tggttggaaa cgatcccggg caagggcctc catgcccact
     7861 cgggaactgg tgcagcgcaa cgcaggaact atgccaaaag ggagcgaacc tcgcttgacg
     7921 ctgcactagc cttgtgcggc aactgcttag gaacccgtgc gccctgcccg aaaagtccag
     7981 tgagagccat tgacgcacca ctatgcagca ttgtgcccca aacaagggca ttggcgcact
     8041 agtttggatc gcgcaggctt cggggcagaa atagcgcctg aaggtcgctc aggaaccggg
     8101 ctcccagcgg agtcggtgcc caatgggcgt ggtcggtcgc caccaggccg cgacgttcgg
     8161 cctcgagcag cgccttctcg atcgccgtag gcggcaggcc ggtacgttcg gtaaaccagc
     8221 gacttggcac tccatccaac agtcgcatgg cattcagcat gaattcgaac ggcagatcgt
     8281 ccctcgccac ttcgctgctc tcggcaagaa attccctgcg acctgcgcgc tcgagataag
     8341 tgcgcgggcc gcgatagcgc acctgacgaa tgatccgatg cggaaacgag atcttcgagt
     8401 gcgcaccggg accgacaccg aggtagtcgc cgaaccccca gtagttgagg ttgtggcggc
     8461 actcccgtgc aggctgcgcg taggccgaga cctcgtagtg gcgatagccg gcagcggctg
     8521 ttcgcccctc gatccatgcc tgcatcgagg cagcggtgtc ctcgtccggc aacggcggtg
     8581 gatgcttggc gaagacggtg ttcggctcca gtgtcaactg ataaagcgac aggtgcggcg
     8641 gtgccagcgc gagcgcctgc tcgacatcgg cttgcgcgcc cgcgaggtcc tgactcggca
     8701 gtccatacat cagatcgatg ttgaagttgt cgaacacccg ccgcgccgtg tcgatcgcac
     8761 gcagggcctg ctctcggtca tggatgcgac ccagtgcctg caacctggag tcgtcgaacg
     8821 actggacgcc gatggacagc cgattgactc cgctcctccg atagtcggca aatcgtccgg
     8881 cctcgaccgt ccccgggttg gcctcgaggg tgacttcgca gtcgccttcc agcgggagca
     8941 gcgcacgcac gtccgacaac aacgtgtcca ggcccctcgc gctcagcagg ctgggtgtgc
     9001 cgccgccgat gaaaatgctg acaatgcgcc gtccccagat cagcggcagt gcggcttcaa
     9061 gatccgatcg cagcgccgtc agatagtcgg cttcggggat cgtgccgggg ccaccctcgg
     9121 gttcatgcga gttgaaatcg caatacgggc acttgcgcac gcaccacggg taatgaacgt
     9181 acagcgacag cggaggcaat gcaggcagcg acagtgttcc cggccgcagg taggccgcga
     9241 tatccgtcgg cgcaggaccg gccagcgcta catctatcgg aatcacgtcc atgcgacctg
     9301 gccctctctc atcaccaggc ggcaagttgc cgcaccagtt ctcgcatggc ccggccgcgg
     9361 tggctgatgc ggttcttctc ctccgcggac aactcggccg cggtgcaacc gcattcgggc
     9421 acaaggaaca acgggtcgta gccgaatccc cccgacccgc gcgggctgtg caggatctcg
     9481 ccctgccagc gcgcatcggc cacgagcggt tccggatcat tcgcagacgc caccgccacc
     9541 agcacgcatg tgtagtgggc acggcgatcg tcatgctcct gcagacggcg cagcagttcg
     9601 gcgttattcg ctgcatcgtc ggccgtcggg ccggcaaagc gtgccgaacg gacgcccggc
     9661 gcgccctgca gcgacgcgac gcacagtccc gagtcgtcg
//
LOCUS       DATIRT010000451         4581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_666466, whole genome shotgun sequence.
ACCESSION   DATIRT010000451 DATIRT010000000
VERSION     DATIRT010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666466"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(291..953)
                     /locus_tag="VLN25_10195"
     CDS             complement(291..953)
                     /locus_tag="VLN25_10195"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HSQ10966.1"
                     /translation="MSELALLAARQRTRVLERQLTLLKAELRLLERSHAETHRRAHFD
                     ALTGLPNRFLLLDRFHQAMAHATRMQTKLALLYLDLDGFKAVNDALGHTTGDELLKRV
                     SVRLQACIRASDTVCRHGGDEFVILLTEVADDAGALLAAEKIRTHLAIPYVIGATAIQ
                     LTTSIGVAVYPIDGSEIEELLQRSDSAMFRNKGDMSSRPNIFRIDQTPATAADGSHMG
                     AG"
     gene            complement(1002..1316)
                     /locus_tag="VLN25_10200"
     CDS             complement(1002..1316)
                     /locus_tag="VLN25_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HSQ10967.1"
                     /translation="MTNLSKYLSPLFVAVALVSVVGCASTPKQEGTAEYMDDTMITTK
                     VKTAILNEPTLKVAEINVETFKGVVQLSGFVSSQAAATKAVEVTRGVGGVKSVKNDMR
                     IK"
     gene            1641..2354
                     /locus_tag="VLN25_10205"
     CDS             1641..2354
                     /locus_tag="VLN25_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HSQ10968.1"
                     /translation="MSTHQHAAPPNRLLAALPPRLSERLIAACDGVDLKLGTVLNEAD
                     ARIAHVYFPTEGFISLMIPVDGKSTLEVGLVGNEGMVGTPLILGMDRSPLRALVQGAG
                     PAWRMSARTFTRELGLSAALRTLLHRYMQVCISQLAQTAACTRFHLVEARLARWLLMT
                     QDRAHDDEFHVTHEFLAFMLGVRRVGVTRAATALQNRKLITYHRGELAILNRPGMEAA
                     SCSCYQADQDSYASLMAGV"
     gene            2715..4355
                     /locus_tag="VLN25_10210"
     CDS             2715..4355
                     /locus_tag="VLN25_10210"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HSQ10969.1"
                     /translation="MHKKSLRHRALALVVGVFVLAFGGWANADPPARIARLGYLAGAV
                     SFSPAGEDDWLQATLNRPLTTGDRLWAEPGARTEIQIGEAMVRMSGGTSVSILNLDDG
                     ISQLQLTQGALNVRVRHLEPTQVFEVDTPNVAFTLRQPGEYRIEVDPDGIATTIVVRK
                     GQGEVYADATAFVIDSRQAYRFMGTGLREYRQAATPRLDAFDRWSNKRDRGYDTSVSA
                     RYVSPDVVGYQDLDANGTWRVNATYGNVWFPNQVATGWAPYRDGHWAWIDPWGWTWVD
                     DAPWGFAVSHYGRWTNFRGTWGWVPGPVRTRAYYAPALVVFIGGENFQLSISPGTVGG
                     VAWFPLGPREVYQPAYPVSRRYFQNINQSNTIINNTVINNTYNNTNVTRVAYANRHVP
                     GAVVAVPATAFVQSQQVSRVAVQVSSEVLVKAPVTSVPFLAPTEESVRGNAAQGGKPP
                     ARVLKRPVVARTVPPAPSVGFATRQQQLVATPRRPLADDARRELKPASTAPVVVKVVA
                     ETQGTAPPQAARPAPIARPPGSAQRPSNLAREISAPNP"
BASE COUNT          852 a         1438 c         1408 g          883 t
ORIGIN      
        1 atcaggaggc cctatgtgcg ccagcgcaca gaccgtcggc cctgctgggg ctattctggg
       61 ttgacggttt ccagccgcaa ggcgctcgcg cttaaaggca actggaatag ttcaaagggc
      121 aggtgtagct caaggcgttc gcagaaaccc acaaggacta ccagccaact cactcggcat
      181 agtccggata actctgctcg acgaaccgct gggcaatccg gcgaggtcgt cgttcagtgg
      241 ctccccgcag cctttccccc ggcggggagc cagcttcttt tgccaacagc tcagcccgct
      301 cccatgtgtg atccgtccgc tgccgtggca ggtgtctggt cgatcctgaa gatgttgggg
      361 cggctcgaca tgtcgccttt gttgcgaaac atggcggagt ccgaacgctg cagaagctct
      421 tctatttcgc ttccgtcaat cggatagaca gccaccccga tgctggtggt caactggatc
      481 gctgtcgctc cgatgacata cgggatggcc aggtgtgtgc gaatcttttc cgcggcaaga
      541 agtgcgcccg cgtcgtccgc aacctctgtc agaagaatga cgaactcgtc accaccgtga
      601 cgacacaccg tgtcgctggc tcggatacag gcctgcaggc gcacagagac ccgtttgagc
      661 aattcgtcgc cggtcgtgtg acccagcgcg tcattgacgg ccttgaagcc gtcgaggtcg
      721 aggtagagca acgcgagttt cgtttgcatc cgcgtcgcgt gcgccatcgc ctgatgaaag
      781 cgatccagca gcaggaagcg attgggaagc ccggtgagcg catcgaaatg ggctcgtcga
      841 tgtgtctctg cgtgcgatcg ctcgagcagc cgcagctctg cctttagcag agtcagctgc
      901 cgttccagaa cgcgtgtccg ctgccttgcc gcgagcagcg ccagttcgct catggtgggc
      961 gcgcggcagg gcgtcatgcg cggacggccg ctggcagccg cctacttgat ccgcatgtcg
     1021 ttcttgacgg acttcacgcc gccaactccg cgagtcacct ccacggcttt cgtggccgcg
     1081 gcctgcgagc tgacgaatcc actcaactgc accacgccct tgaacgtttc aacattgatt
     1141 tcggcaacct tcagggtggg ttcgttaagg atggccgtct tcaccttggt ggtgatcatg
     1201 gtgtcgtcca tgtactcggc cgtcccttcc tgtttcggag tggaagcgca acccacgacc
     1261 gataccaatg caacagcaac gaacagcggg gagagatact tgctcaggtt ggtcattctt
     1321 cgtactccgg ttgtattcag cgatttctac caaaagcaca ctatgggcga agtggcattg
     1381 ctcgtcggtg cgctgccgca cattcgcgcc ggggggcaaa cgagttcaca tgttccgcag
     1441 cccccgcgcc tgggctacgc acgcatcccc gttgaagggc gcgcggagga acagaaaatt
     1501 tgcctggtgc gtgcgctggc gaacaaacag atccccggat caggcctacc gtcggtttct
     1561 agtggctgat ctggccatcg ttctgtgacg tgtgcagcgg ctagggcacg gcacagcctt
     1621 tgagaggttt gtcgccacct atgtcaaccc atcaacacgc tgcgccgccg aaccgacttc
     1681 ttgccgcgct accgccgcgt ctttcagagc ggctgattgc agcctgcgat ggcgtcgatc
     1741 tcaagctcgg taccgttctg aacgaggccg acgcgcgcat tgcgcacgta tattttccga
     1801 ccgagggctt catttcgctc atgatcccgg tcgacgggaa gtccactctc gaagtcggac
     1861 tggtgggcaa cgagggcatg gtcggaacgc cgctgatcct gggcatggac cggtcgccac
     1921 tgcgtgcgct ggtgcaaggc gcgggtcccg catggcggat gagcgcccgg acatttacac
     1981 gcgaacttgg attgagtgcg gcgctgcgca ccttgctgca tcggtatatg caagtttgta
     2041 tcagtcaact ggcacagact gccgcttgca cccgctttca cctggtcgag gcacggctgg
     2101 cccgctggct gctgatgacg caggaccgcg cgcatgacga cgagtttcat gtgacgcacg
     2161 agtttctcgc cttcatgctc ggggtccgac gcgtaggagt cacgcgggcc gcgaccgcgc
     2221 tgcagaatcg taagctgatc acttaccacc gcggtgaact ggcgatcctc aaccgccctg
     2281 gtatggaagc ggcatcctgc agctgctacc aggccgatca agacagctac gcgagcctga
     2341 tggctggcgt gtagtcgcgc gcagaagcat ctcaccgttg gttcgttctc cactgcggcg
     2401 tgccctgccc tagttgatag cgctgcccgg aagtcttctc aacgtctgcg acctagccgc
     2461 aggcgtatgg ctccaggctg cgacgtttcg ggcgtgcagt agccgtggcc ggagttgatc
     2521 ggtgcgctac cgaacagacg tctggcgccg cgctccgtat ggtgttcgac atcctcttgg
     2581 caagacgaga cgggttaggg caccacagtt cgcggatccg tttcggtacc gagccagttg
     2641 tcggttagat ccgttgctgc gcagcctcgc ggttctatcg gtcgggtcga cgcgcatttt
     2701 cttaagaaag cgacatgcac aaaaaatctc tccgccacag ggcgctcgcc ctcgtggtgg
     2761 gtgtcttcgt actggccttc gggggctggg ccaacgctga tccgccagcg cgcattgcgc
     2821 gcctcggtta cttggcggga gcggtcagtt tctcgcccgc aggtgaggat gactggttac
     2881 aggcaacgct caatcggccg ctgacgaccg gcgaccgcct ctgggcggag cccggcgcgc
     2941 ggaccgaaat ccagatcggc gaggccatgg tccgcatgag cggtggaacc agcgtatcca
     3001 ttctcaatct cgacgatggg atctctcaac tgcagctgac acagggcgca ctgaatgtcc
     3061 gcgtgcgcca cctcgaacct acccaggtgt tcgaggtcga cacgccaaat gttgccttta
     3121 cccttcgaca acccggcgaa taccggatcg aagtggatcc cgatggcatt gcgaccacga
     3181 ttgtggtgcg caaaggccaa ggcgaggtgt acgccgacgc cacagcgttc gtcatcgact
     3241 cgcggcaggc ttatcgcttc atgggaacgg gtttgcgcga gtatcggcaa gccgctacac
     3301 cccgcctcga cgcattcgac cgctggtcga ataaacgcga tcgcggctac gacacttcgg
     3361 tctccgcccg ttacgtatca ccggacgtcg tcggctatca ggacctggat gcaaacggta
     3421 cctggcgcgt caacgcgacc tacggcaacg tgtggttccc aaatcaggtg gccacgggct
     3481 gggcgcctta ccgcgacggc cattgggcgt ggatcgaccc ctggggttgg acctgggtgg
     3541 atgacgcccc gtggggcttc gccgtgtcgc actacggccg ctggacaaat ttccgcggga
     3601 catggggttg ggtgcccggc cctgtgcgca ctcgcgccta ctacgccccg gctctcgttg
     3661 tgtttatcgg cggcgagaac tttcaactct ccatttcccc cggcaccgtc ggtggcgttg
     3721 cgtggttccc gctggggccg cgggaggtct atcaaccggc ttatccggtg agccgccgat
     3781 acttccagaa catcaaccaa agcaacacga tcatcaacaa cacggtcatc aacaacacct
     3841 acaacaacac gaacgtgacc cgagtcgcct atgcgaaccg gcacgtcccg ggagccgtgg
     3901 tcgctgtgcc ggcaacggct ttcgtgcagt cgcagcaagt gtccagggta gcggtgcaag
     3961 tgtcctccga agtgctggtc aaggcgccgg taacgagtgt tccattcctg gccccgacgg
     4021 aagaaagcgt gcgcggcaat gctgcacagg gcggcaagcc gccagcaagg gtgttgaaga
     4081 ggccggtcgt cgcacgaacg gttccaccgg caccgagtgt cggctttgcg acgcgacagc
     4141 agcaactggt ggcaacgcct cgcaggccgc tcgccgacga cgcgcgcagg gagttgaagc
     4201 ctgcatcgac agcgcctgtc gtcgtgaagg tggtcgcgga gacgcaaggc actgcgccac
     4261 cgcaagccgc gcgacccgca ccgatcgcgc gaccgccggg cagcgcgcaa aggccgagca
     4321 acctcgcaag ggaaatcagc gcgcctaatc cgtagcaacg ccgcccacgc cacccaagcg
     4381 cgaggcagaa gagcgcttgc aaagttaaaa ccgcgaacag cgtcgcgagt gaagtaatgg
     4441 cccgcatcgg ctcatgattg ccatctcttc ctatgagcgg tagcgcacgg actccactgc
     4501 ggctccaacg tacgctcagt aggtcaatca ttcctcaccc gcgctgtctc tacgcgcagc
     4561 gcccgaagtg gcgcgggaaa c
//
LOCUS       DATIRT010000452         2413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1060466, whole genome shotgun sequence.
ACCESSION   DATIRT010000452 DATIRT010000000
VERSION     DATIRT010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060466"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VLN25_10215"
     CDS             <1..531
                     /locus_tag="VLN25_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSQ10970.1"
                     /translation="SAVRSGGVLLAAFIGGASFFFGPILGAIVFTFFAVALSDYTKAW
                     QLYLGVFFVVLVMFAPGGLSSLLLMNLRVMKFRKFKRLRDPYVGVLLTALAMTLGVIM
                     LIELTYHVTLDTGQGPVTWLFGIPMDTATPGPWVLAAFVLLAGAVAFEYYRRHFSRCW
                     DEIQIEIEDWVRDNPA"
     gene            528..1280
                     /locus_tag="VLN25_10220"
     CDS             528..1280
                     /locus_tag="VLN25_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSQ10971.1"
                     /translation="MNALELDGVRKSFGKTEIIRGIDLAIPHGERHAIIGPNGAGKST
                     LFNLISGRFGASAGSIRLHGQPITGRKPFEINRLGLARSFQITNLFHRLSVFENLRCA
                     VLWSLGHRYAFWKNVNKLSDTEERAESLLERVGLKRRRDTLAGLLTYAEQRALEIGIT
                     IAGGASVILLDEPTAGMSRAESKRAVELIRDVTQGKTLVMVEHDMSVVFGLADRISVV
                     VYGQVIASGPPAEIRASAAVKEAYLGQHGADA"
     gene            1336..2079
                     /locus_tag="VLN25_10225"
     CDS             1336..2079
                     /locus_tag="VLN25_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSQ10972.1"
                     /translation="MSSAPMLRVENLQAYYGKSHVLHGVDLEVGHGEIVSLLGRNGVG
                     RSTTAKAIMGLVDATGMIEYGPHDLTRLKTFEIAHLGIGYVPESRDVFPTLTVEENLK
                     LGVKRGTSAKWSYADVYRLFPRLQDRAGTQAGVLSGGEQQMLTLARTMMGGPDFVIID
                     EPTEGLAPMIVELVGKFLLELRDRGVSILLIEQKLAIAMQISQRVYVMGHCHMVFHGT
                     PDELKANKLIRQEWLEVCAPGGASRSFPQ"
     gene            2076..>2413
                     /locus_tag="VLN25_10230"
     CDS             2076..>2413
                     /locus_tag="VLN25_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HSQ10973.1"
                     /translation="MTTHYERRGEVAVLQIDNPPVNGLGHVSRKALLDGLERALDDGA
                     VKVVVVTGTGTVFSGGADIREFNTPAALAEPSLLQLIDAVEGSPKPVIAAINGVCMGG
                     RLELSLGCHYR"
BASE COUNT          417 a          733 c          815 g          448 t
ORIGIN      
        1 agcgccgtgc gctcgggagg cgtgctgctc gcggccttca tcggcggcgc aagtttcttc
       61 ttcggtccga tcctcggggc gatcgtcttc accttctttg ccgtggcgct gtccgattac
      121 acgaaggcct ggcagctcta cctcggcgtg ttctttgtcg tgctcgtcat gttcgcgcca
      181 ggcggactgt cgagcctgct gctgatgaac ctgcgggtga tgaagttccg caagttcaag
      241 cgcctgcgcg atccatacgt cggcgtgctg ctgacggccc ttgcaatgac cctgggcgtc
      301 atcatgctga tcgagctcac ctaccacgtc acgctcgaca cagggcaggg gccggtcacg
      361 tggctgttcg gcatcccgat ggacactgct acgcccggtc cgtgggtgct ggctgccttc
      421 gtactgctgg cgggggccgt ggccttcgag tattaccggc gccatttttc ccgttgctgg
      481 gacgagatcc agatcgagat cgaggactgg gtccgggaca accccgcatg aatgcgctcg
      541 aactggacgg ggtgcgcaag agcttcggaa agacggagat catccgcggc atcgacctgg
      601 ccattcccca tggcgagcgc cacgcaatca tcggtccgaa cggtgccggc aagtcgacgc
      661 ttttcaacct gatcagcggg cgctttggcg cgtccgcggg cagcatccgg ctgcatggcc
      721 agcccattac ggggcgcaag ccgttcgaga tcaaccggct tgggctcgcg cgcagcttcc
      781 agattacgaa cctctttcac cgcctgtcgg tgttcgagaa cctgcgctgc gcggtgctgt
      841 ggtcgctcgg gcatcgctat gcgttctgga agaacgtgaa caaactcagc gatacggaag
      901 agcgcgccga gagcctgctc gaacgcgtcg gcctgaagcg ccggcgcgac accctggcag
      961 gcctgcttac ctatgccgag cagcgcgcac tcgagattgg catcacgatt gctggcggcg
     1021 ccagcgtgat cctgctcgat gagccgacgg ccggcatgag ccgggccgag agcaaacggg
     1081 ctgtcgagct gatccgagac gtgacccagg gcaagacgct ggtcatggtc gaacacgaca
     1141 tgagcgtggt atttggcctg gccgatcgca tcagtgtcgt cgtctatggc caggtgatcg
     1201 cgtcgggtcc cccggcggag atccgtgcca gtgcggcggt caaggaagcc tatctaggac
     1261 agcacggggc ggacgcatga gcccttcgat cggtcacggt gacgcgcatc aacgggcggc
     1321 gctccgaggt tcacgatgag cagcgcgccg atgctgcgtg tcgagaacct gcaggcctac
     1381 tacggcaaga gccacgtgct gcacggtgtt gacctcgagg tcggtcacgg cgagatcgtc
     1441 agcctgctgg gtcgcaacgg tgtcgggcgc tcgaccacgg ccaaggcgat catggggctg
     1501 gtcgatgcga ccggcatgat cgagtacggg ccgcacgacc tgacgcgact gaagactttc
     1561 gagatcgcgc atctggggat cggctatgtg ccggaaagcc gcgacgtgtt cccgacgctc
     1621 acggtcgagg aaaacctgaa gctcggcgtc aagaggggca cgtcggcgaa gtggagttac
     1681 gcggacgtct atcggctttt cccgcgtctg caggatcgag cgggcacgca ggccggtgtg
     1741 ctgtccggag gcgaacagca gatgctgacg ctggcgcgta ccatgatggg cggtccggat
     1801 ttcgtgatca tcgacgagcc gaccgaggga ctcgcgccga tgatcgtcga actggtgggc
     1861 aagttcctgc tcgagctgcg cgatcggggc gtgtccatcc tgctgatcga gcagaagctg
     1921 gcaatcgcca tgcagatctc gcagcgggtg tacgttatgg ggcactgcca catggtgttt
     1981 catggcacgc ccgatgaact gaaggccaac aaactgatcc gccaggagtg gctcgaagtc
     2041 tgtgcccccg gcggcgcctc ccggagtttt cctcaatgac gacccactac gagcgccgcg
     2101 gtgaagtcgc ggtgctgcag atcgacaatc cgccggtcaa tggcctcggc cacgtgtccc
     2161 gcaaggcgct gctcgacggc ctggaacgag cgctggatga cggggcggtc aaggtcgtcg
     2221 tggtgacggg taccggaacg gtgttttccg gcggcgcgga catacgcgaa ttcaacaccc
     2281 cggcggcatt ggccgagccc agtttgctgc agctgatcga tgccgtggaa ggctctccca
     2341 agcccgtgat cgccgccatt aacggcgtat gcatgggagg caggctggag ctgtcgctgg
     2401 gttgccacta ccg
//
LOCUS       DATIRT010000453         9415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_961517, whole genome shotgun sequence.
ACCESSION   DATIRT010000453 DATIRT010000000
VERSION     DATIRT010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9415
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961517"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="VLN25_10235"
     CDS             complement(<1..1029)
                     /locus_tag="VLN25_10235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HSQ10974.1"
                     /translation="MTPLALACLGALACLLAWPGEAAALIRGIALQPDARERLWLSAF
                     SGFTLGLAIGLMVYEVRARGVSIALRRLTDLAASVDGRFPEETPRGKIRRELERLSDE
                     VLYSARRMTKERKEFDSQATAWQAMFAATLDAMFVLDAAGKVKELNPAAERQFKITAA
                     EAVGSHLADLLFPLPHRSLDNAAFMQDIAAGKAVGRRQELIVHCGQRQFPVEMAIAEF
                     HVGEDLGLIVTARDISAHRQTRADLKRVREQSDRLQARLRSELASRRLVRPTVESMTQ
                     VETAVAAPTGGTPFTLEDACGDVIRKLVVKAERKGLGFRYEDTEVQGMGLIGDASRLR
                     AVASELIDN"
     gene            complement(1151..2383)
                     /locus_tag="VLN25_10240"
     CDS             complement(1151..2383)
                     /locus_tag="VLN25_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSQ10975.1"
                     /translation="MNAMMVLRVALKALRVNKLRSSLTMLGMIIGVAAVIAMLAIGGG
                     AQERVRDQLKNLGSNLMLIIPGSTTASGVRLGTGAAQTLTEEDALAIALEVEGVVVAA
                     PSLRGSGQVVVGNANWSTQFFGTNNDYLIARDWPLASGRLFEPNEIASSGKVALIGLT
                     TAQQLFGDADPVDQVIRVRKVPLTIIGVLARKGQNALGQDQDDIVLMPISTARNRVLG
                     QTQGKQRRVGTISVKTADGADMKAIEDKIKELLRQRMKVQPGADDPFTIRNLTEILQA
                     QEAASRVLTLLLGAVASVSLLVGGIGIMNIMLVSVTERTREIGLRMAVGARGRDILKQ
                     FLVEEVTLSMIGGALGILLGVVASFAVGEFADWRTDITPQAVALAVGFSAAVGVFFGF
                     YPARKASRLLPIEALRYE"
     gene            complement(2394..3131)
                     /locus_tag="VLN25_10245"
     CDS             complement(2394..3131)
                     /locus_tag="VLN25_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSQ10976.1"
                     /translation="MLIETGDLRKDYTLGDTVVHALAGVTLSIDAGEFVAIMGPSGSG
                     KSTFMNMVGCLDRPSSGRYWLAGEDVAAMDSDALAAVRNQRIGFVFQQFNLLARTSAL
                     ENVELPLLYANVPIRDRHTRALEKLGRVGLGERADHQPSQLSGGQQQRVAIARALVND
                     PSLILADEPTGALDSRTSVEIMALLQQLNSGGMTIVLVTHENDVAAFAGRVVHFRDGK
                     VVSDTSQTPRSAMAELERLDRGAAVAA"
     gene            complement(3237..4766)
                     /locus_tag="VLN25_10250"
     CDS             complement(3237..4766)
                     /locus_tag="VLN25_10250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSQ10977.1"
                     /translation="MFRNFRLWIALALLAAVLGAGFWWSTRASADAPSYRLAKVERGP
                     LTATVSSTGTLNPVTSVQVGTQVSGQIKELFVDFNSQVKKGELIARIDPETLQYRVRQ
                     AEADLEAGRSSLNRARVSLANTKRDLDRTKELVARNFTSPAELDRAQSTFDLGAAEVR
                     TAQAVVQQRDAQLATAKVDLGRTEIRAPVDGVVIKRSVDVGQTVAASLQAPELFVIAK
                     DLRDMQVDTSIDEADVGRIRVGQKASFTVDAFPGRTFNGEVKTVRKAPQNTQNVVTYI
                     AIVSANNDRGELLPGMTANVRIVTDTRDSVLKAPNGALRFRPSGAAAAGADASKTTEA
                     TDKSAGKAGAAGAASAAGAAGGSPITAFRERLITELKLDAEQQQRLEPIFAEMRNKFM
                     GLRELPEEARAKAGGAIRAELRAKIEDTLKPEQKARYAEIAAEFAGRAGGGQTTRGRL
                     WVLDGGKPRGIDVRVGLSDGTMTEVSGDGVSEGLEVVVGQQGGATSTPAQKGAPPRMF
                     F"
     gene            complement(4908..5579)
                     /locus_tag="VLN25_10255"
     CDS             complement(4908..5579)
                     /locus_tag="VLN25_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HSQ10978.1"
                     /translation="MNLPTLSLLETRVLGVLVEKEHTVPDIYPLSLNALTAGCNQKNN
                     RDPVLSATEAEVQAAIDRLKPLSFVIESSGSRVMRYSQNVGRVLRVPSESVALLATLW
                     LRGPQTAAELRSASERLHRFADVSSVEGYLDQLATRAAEAGGALVTLLARAPGAREPR
                     WAQLLSPTPPTLPAPVMASTSAGNSADVGIPAAADELRAEIARLRDEMQELRARVERL
                     EGGAV"
     gene            complement(5590..7296)
                     /locus_tag="VLN25_10260"
     CDS             complement(5590..7296)
                     /locus_tag="VLN25_10260"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HSQ10979.1"
                     /translation="MSDIQLMILRRMRAPLIVLIVTYAISVFGLVLMPGVDPQGNPWH
                     LSLFDAFYVMSYTATTIGFGEVPYPFSYAQRLWMTVSIYISVTGWAYALGSIFALVQH
                     PAFKEAWARSRFEAGVRRLVDRYFVICGFGQSGKRLAGAFDRLGYAIVVIESSAERLR
                     PLYLRENRQPAVGLVGDARSPALLEASGIRRPNCEGVIVLTGSDEVNQTIAIETRVLA
                     PEVTVLARIKSDTVCETLEAFGGVTVVNPYETFGVNLGLAIAKPDVLRLEEWMTAVPG
                     TEPPRRIEAPRGHWVLAGHGRFGKTLSAALERAGSTCTVIDPDPARCGVDGVVGTGMA
                     PDALQRAGIQDASGLIVSTDVDANNLAIVTAARRLKPKLFVVIRQNQETNRSLIDAAR
                     ANMHFVQAQLMAHECLQVLTTPLLNRFLLRAREQPNAWAVGVCGRLHDVIGHHVPHVW
                     AVTCDTKLLGMRRALIEVSEPALTISHLLCDPDDRLTRLQATPLLLLRIGDDVLLPDE
                     GTALEAGDRVLFAGASDARLVQSRLLEDDVAIDYVRTGYERPRTWIGRWLQRPVTNPV
                     AG"
     gene            complement(7328..7711)
                     /locus_tag="VLN25_10265"
     CDS             complement(7328..7711)
                     /locus_tag="VLN25_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6394 family protein"
                     /protein_id="HSQ10980.1"
                     /translation="MNLEKVIFGAFVILACTLNFGFFIGDLGNPDYHHAGELGATIVV
                     NLIAMTLKFGDRTHIGAVHLATSIVAVLQLLAAAVVWVWSTRVANLPEAGEVMATVVS
                     LSGGALVANFISVALLIGETLRRVR"
     gene            complement(7830..8081)
                     /locus_tag="VLN25_10270"
     CDS             complement(7830..8081)
                     /locus_tag="VLN25_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10981.1"
                     /translation="MCVRLKDYIANRWVKIGLVLVLLGWGPLLAVILLAAIGLWPDPN
                     PNPIGLGLLFFVTFWPAVACLGFGAFQVQRRRQSPDRGS"
     gene            complement(8103..>9415)
                     /gene="thiC"
                     /locus_tag="VLN25_10275"
     CDS             complement(8103..>9415)
                     /gene="thiC"
                     /locus_tag="VLN25_10275"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HSQ10982.1"
                     /translation="TPEFVRDEIARGRAIIPCNINHAELEPMIIGRNFLVKINANIGN
                     SAVTSSIGEEVEKMTWAIRWGADTVMDLSTGKHIHETREWILRNSPVPIGTVPIYQAL
                     EKVDGKAEDLTWEIFRDTLIEQAEQGVDYFTIHAGVRLPYIPMTAKRMTGIVSRGGSI
                     MAKWCLSHHKESFLYTHFEEMCELMKAYDVAFSLGDGLRPGSIYDANDEAQMAELATL
                     GELTQVAWKHDVQVMIEGPGHVPMHMIKHNMDEQLRLCHEAPFYTLGPLTTDIAPGYD
                     HITSAIGAAMIGWYGTAMLCYVTPKEHLGLPNKKDVKDGIMAYKIAAHAADLAKGHPG
                     AQMRDNALSKARFEFRWEDQFNLGLDPDTAKDFHDETLPKDAMKSAHFCSMCGPHFCS
                     MKITQDVRDYAAKQGVSAEQALNKGMQEKAVEFVQKGGEIYRKA"
BASE COUNT         1626 a         3185 c         2964 g         1640 t
ORIGIN      
        1 gttgtcgatg agttcggagg cgaccgcgcg caggcgcgac gcatcgccga tcaggcccat
       61 gccctggacc tcggtatctt cgtagcggaa gcccaggccc ttgcgctcgg ccttgacgac
      121 gagcttgcgg atcacatcgc cgcaggcgtc ttcgagggtg aacggcgtac cgccggtggg
      181 agccgcgacg gctgtttcga cctgtgtcat cgattcgacc gtcggacgaa ccagccgacg
      241 cgatgcgagt tcggaacgca ggcgcgcctg caggcggtcg gactgctcgc gaacgcgctt
      301 cagatcggcc cgcgtctggc ggtgcgccga gatgtcgcgg gcggtgacga tcagtcctag
      361 atcttcgccg acgtggaatt cggcgatcgc catttcgacc ggaaactggc gctggccgca
      421 atgcacgatc agttcctgcc gccggcccac cgccttgccg gccgcgatgt cctgcatgaa
      481 ggcggcattg tcgagcgacc gatgcggcag cgggaagagc aggtcggcca ggtggctgcc
      541 gaccgcctcg gctgcagtga tcttgaactg gcgttcggcg gccggattga gttccttgac
      601 cttgcccgcc gcgtcgagca caaacatcgc atcgagcgtg gcggcaaaca tggcctgcca
      661 ggcggtggcc tgactgtcga actccttgcg ctcctttgtc atgcggcggg cgctgtacag
      721 cacctcatcc gacagccgct cgagttcgcg acggatcttg ccgcgcggtg tctcctcggg
      781 gaagcgtcca tcgacgcttg ccgcgagatc cgtgaggcgc cgcagcgcga tagagacccc
      841 gcgggcgcgc acttcgtaga ccatcagtcc gatcgcaagg ccgagcgtga agccgctgaa
      901 agcggacagc cacaggcgct cacgcgcgtc gggctgcagc gcgattccgc ggatcagcgc
      961 ggcggcctcg ccgggccagg ccagcaggca cgcgagcgcg cccaggcagg ccagtgcgag
     1021 tggcgtcaag cacagagtac gaatcggtcg aggcatgggg ggcgcggccc cgggatcaag
     1081 ggcccgggcc gtgaccatgc tgccgcagca aaggccgtcc agcccgtgaa taagcgcggc
     1141 gccaggccgc ctattcgtag cgcagcgcct cgatcgggag gaggcgtgac gccttgcggg
     1201 caggatagaa gccgaagaac acgcccaccg cggccgaaaa gcccacggcc agcgcaaccg
     1261 cctgcggcgt gatgtcggtg cgccagtcgg cgaactcgcc gaccgcgaac gaggcaacga
     1321 caccgagcag gatgccgagc gcgcccccga tcatggacag cgtcacctcc tcgaccagaa
     1381 actgcttgag aatgtcgcgc ccacgcgcac cgaccgccat gcgcaagccg atctcgcgcg
     1441 tgcgttcggt cacgctgacc agcatgatgt tcatgatgcc gataccgccg accagaagtg
     1501 acaccgacgc caccgcaccc agcagcagcg tcagcacgcg gctggccgct tcctgcgcct
     1561 gcagaatttc ggtgaggttg cggatcgtga acgggtcgtc cgcgcccggc tggaccttca
     1621 tgcgctgccg cagaagttcc ttgatcttgt cctcgatcgc cttcatgtcc gcgccgtcgg
     1681 cggtcttgac cgagatcgtg ccgacacggc gctgcttgcc ctgcgtctgg ccgagcacgc
     1741 ggttacgcgc ggtggagatc ggcatcagca caatatcgtc ctgatcctgc ccgagcgcat
     1801 tctggccctt gcgcgccagc acgccgatga tcgtcagcgg caccttgcgc acgcggatga
     1861 cctgatcgac cggatcggca tcgccgaaca gctgctgcgc ggtggtcagg ccgatcagcg
     1921 cgaccttgcc ggagctggca atctcgttcg gctcaaacag gcgacccgaa gccagaggcc
     1981 agtcacgtgc gatcaggtaa tcgttgttcg tgccgaaaaa ctgcgttgac cagttcgcgt
     2041 tgccgacgac cacctggccc gagccgcgca gcgatggcgc ggcgacgacg actccttcga
     2101 cctcgagcgc gatcgcgagc gcgtcttcct cggtcagcgt ctgcgcggcg ccggtgccca
     2161 gccgcacgcc actggccgtg gtcgaacccg ggatgatcag catcaggttc gagcccaggt
     2221 tcttcagctg gtcgcgcacg cgttcctgcg ctccgccgcc gatcgccagc attgcgatca
     2281 ccgccgccac gccgatgatc atgcccagca ttgtcaacga tgagcgcagc ttgttcaccc
     2341 gcagcgcctt gagcgccacg cgcaacacca tcattgcgtt catcgcagcg tcctcatgca
     2401 gccaccgccg cgccgcgatc gagacgctcg agctccgcca tcgccgaacg cggcgtctgg
     2461 ctcgtgtcgc tcaccacctt gccgtcgcgg aagtgcacca cccgcccggc gaaggcagcg
     2521 acgtcgttct cgtgcgtcac cagtacgatt gtcatgccgc cgctgttcag ttgctgcagc
     2581 agggccatga tctctacact ggtgcgggaa tcgagcgcgc ccgtcggctc atccgccagg
     2641 atcagtgacg gatcgttgac cagggcgcgg gcaatcgcga cgcgctgctg ctgtccgccc
     2701 gagagctgcg acggctgatg gtcggcgcgc tcgccgagcc caacccggcc gagcttttcg
     2761 agcgcgcgag tatggcggtc cctgatcggc acgttggcat acagcagcgg cagctcgacg
     2821 ttctccagcg cactggtgcg cgccagcagg ttgaattgct ggaacacaaa gccgatgcgc
     2881 tgattgcgaa cggccgccag cgcgtccgag tccatggcgg cgacgtcctc gccagcgagc
     2941 cagtagcggc ccgagctcgg tcgatcgaga cagccgacca tgttcatgaa cgtcgacttg
     3001 cccgagcccg acggtcccat gatcgcgacg aattcgcccg cgtcgatcga gagcgtcacg
     3061 cccgcgagcg cgtgcacgac ggtgtcgccg agcgtgtagt ccttacgcag gtcgccggtc
     3121 tcgatcagca tggttgggtt cttgcgttgc agttgcctca aaaggtttga ctggctttgc
     3181 cagcgaactt tttcaaatcg cgcgcgtgaa agtagctttg cacgttcgcg cgaccatcaa
     3241 aagaacatgc gcggaggtgc gcccttctgc gccggagtgg aagtcgctcc gccctgttgt
     3301 ccgacgacga cctcgagtcc ttcgctgacg ccgtcgccgc tgacctcggt catcgtgccg
     3361 tccgacaggc ccacgcgcac atcgatgccg cgcggcttgc cgccatccag gacccagagt
     3421 ctgccgcgtg tcgtctggcc gccgccggcc cgtccggcaa actcggcggc aatctccgca
     3481 tagcgtgcct tctgctcggg cttgagtgtg tcctctatct tcgcgcgcaa ctcggcgcgg
     3541 atcgcaccac ccgctttcgc ccgtgcttcc tccggcaatt cgcgcaggcc catgaacttg
     3601 ttgcgcattt cggcaaagat cggctcgagt cgctgctgct gctctgcatc gagcttcagt
     3661 tccgtgatca agcgctcgcg gaacgcggtt atcgggctcc cgcctgccgc accggccgca
     3721 cttgccgcac ccgcggcgcc cgccttgccc gccgacttgt cggtagcctc cgttgtcttg
     3781 ctcgcatcag cgcctgcagc ggccgcgccg ctgggccgga acctgagcgc gccattcggt
     3841 gccttaagca cggagtcgcg cgtgtcggtc acgatgcgca cattggcggt cattcccggc
     3901 agcagttctc cgcgatcgtt gttcgccgac acgatggcga tgtaggtcac gacgttctga
     3961 gtgttctgcg gcgccttgcg cacggtcttc acctcgccgt tgaacgtccg tcccggaaac
     4021 gcatcgaccg tgaagctcgc cttttgcccg acgcggatgc gcccgacatc ggcctcgtcg
     4081 atcgaggtgt cgacctgcat gtcacgcagg tccttggcga tcacgaaaag ctcgggcgcc
     4141 tgcaaactgg cggccacggt ctgcccgacg tcgacactgc gcttgatcac cacgccatcg
     4201 accggggcgc ggatctcagt gcgtcccagg tcgacctttg cggtcgcgag ttgcgcatca
     4261 cgttgctgga cgaccgcttg tgcagtgcgc acttccgcgg cccccaggtc gaaagttgac
     4321 tgcgcacgat caagctctgc cggcgacgtg aagttgcgcg caaccagttc cttggtgcgg
     4381 tccagatcgc gcttcgtatt ggccagcgaa acccgcgcgc ggttcagcga agaacgcccg
     4441 gcctcgagat ccgcctccgc ctggcgcacc cggtactgca acgtctccgg atcgatgcgg
     4501 gcgatcagtt cacctttctt gacctgtgag ttgaagtcga cgaaaagttc cttgatctgc
     4561 cccgagacct gcgtgcccac ctgaaccgac gtcaccggat tgagcgtgcc ggtcgacgac
     4621 acggtggccg tcaaagggcc ccgctcgacc ttggccagcc gatagctcgg cgcgtcagca
     4681 ctcgctcgtg tagaccacca gaagcccgcg ccgagcacgg cagccagcag cgcaagcgcg
     4741 atccaaagtc gaaagttcct gaacacgaag ccatcccgcg gtgatgacga gccgcgattc
     4801 tacgcagcga ctcacgctgc ccggcgagat gtaactcctc gatacagcgc ggaaacctcg
     4861 cagaaatgtc ggcccaggta cgcggggtct ccgcgctcgg accgcgctta gacggctccg
     4921 ccctcgagac gttcgacgcg cgcacgcaat tcctgcattt catcgcggag ccgggcaatt
     4981 tcggcgcgaa gctcgtcggc agcggccgga atgccgacgt ccgcggagtt gccggccgat
     5041 gtcgatgcca tgaccggtgc aggcagagta gggggtgtag gcgacagcag ctgcgcccag
     5101 cgcggctcac gcgccccggg ggcgcgtgcc agcagagtga cgagcgctcc gcccgcctcc
     5161 gcagcgcggg tcgccagctg gtccaggtag ccctcgaccg aactgacatc cgcaaagcgg
     5221 tgcagccgct cgctcgccga acgcaactcg gcggccgtct gcgggccgcg cagccacagc
     5281 gtggcaagca gcgcgaccga ttcggacggc acgcgcagca cgcgaccgac attctgcgag
     5341 tagcgcatca cgcgcgagcc gctcgactcg atcacgaacg acagcggctt cagccgatcg
     5401 atggccgcct ggacttcggc ctcggtcgca gacagcaccg ggtcgcggtt gttcttctga
     5461 ttgcagccgg cggtcagcgc gttcaacgac aacggataga tgtcgggaac cgtgtgctcc
     5521 ttttccacca gcacacccag cacccgggtc tcgagcaagg aaagcgtcgg caagttcatc
     5581 actctcttcc tagccggcga ccggattggt caccggtcgc tgcagccacc ggccgatcca
     5641 ggtccgtggg cgctcgtatc cggtgcggac atagtcgatc gcgacgtcgt cctcaagcag
     5701 gcgtgactgc acgagccggg cgtcgcttgc gccggcgaag agcacccggt cccctgcttc
     5761 gagcgctgta ccttcgtccg gcagcagcac gtcgtccccg atgcgcagca acagcaacgg
     5821 cgtcgcctga agccgagtaa gtcgatcgtc gggatcgcaa agcaggtgcg aaatcgtcag
     5881 tgcgggttcc gaaacctcga tcagcgcccg ccgcataccg agcagcttcg tgtcgcaagt
     5941 gacggcccac acgtgcggca cgtgatggcc gatcacgtcg tgcagccggc cgcagacacc
     6001 gacagcccaa gcattaggct gttcgcgcgc cctcaacagg aaccggttca gcagcggcgt
     6061 agtgaggacc tggagacatt cgtgtgccat caattgcgcc tggacgaagt gcatgttcgc
     6121 tcgcgccgca tcgatcaggc tgcggttcgt ctcctggttc tgccggatca ccacgaagag
     6181 cttcggcttg agccgtcgcg ccgccgttac gatcgccagg ttgttggcgt cgacgtcggt
     6241 agagacgatg agcccgctgg catcctggat gccggcacgc tgcagggcgt ctggcgccat
     6301 accggtgccg acgaccccgt cgactccaca gcgggccgga tccggatcga tgaccgtgca
     6361 tgtcgacccc gcacgttcaa gcgcagcgct cagggtcttg ccgaatcgtc cgtgacccgc
     6421 cagcacccaa tgtccacgcg gcgcttcgat gcgtcgcggc ggctcggttc ccggcactgc
     6481 ggtcatccac tcttccagcc gcagaacgtc cggcttggca atggccagac cgagattgac
     6541 gccgaaggtc tcgtacgggt tcacgaccgt caccccaccg aatgcctcga gcgtctcgca
     6601 gacggtatcg gacttgatgc gcgcaagcac ggtcacctcc ggcgccagca ccctggtctc
     6661 gatggcaatc gtctggttca cttcatcgct gccggtcagc acaatcacgc cctcgcagtt
     6721 cggccggcgg attccggacg cttccagcag tgcaggcgaa cgtgcatcgc cgaccaatcc
     6781 tacggccggc tgccggtttt cccgcagata cagcggtcgc agtctctcgg ctgaggactc
     6841 gatgacgacg atggcgtaac ccaggcgatc aaacgcgcca gccagcctct tgccgctctg
     6901 gccgaagccg cagatcacga aatagcggtc gaccagccga cgcacgccgg cctcgaagcg
     6961 gctgcgtgcc caagcctcct tgaatgccgg gtgctggacc agcgcgaaga tggaaccgag
     7021 cgcatacgcc cagccggtca cggaaatata gatgctgacc gtcatccaca ggcgctgcgc
     7081 ataggaaaaa gggtaaggga cttcgccgaa gccgatcgtc gtcgccgtgt agctcatgac
     7141 ataaaaggcg tcgaacaggc tcagatgcca gggattgcct tgaggatcga cacccggcat
     7201 cagcaccagg ccaaacaccg agatcgcata ggtcacgatc agcacgatca gcggcgctcg
     7261 cattcgccgc aggatcatca gctggatatc ggacatggcg tctcgccgcg tcgagtcggt
     7321 tgacgtgtca gcggacgcgt ctcagcgttt cgccgatcag caacgccacc gagatgaagt
     7381 tcgctaccag cgcgcccccc gacagcgaca cgacagtcgc catgacctcc cccgcctccg
     7441 gcaggttggc cacccgcgtc gaccagaccc agaccactgc cgccgcgagc aactgcaaca
     7501 cggcgacgat gcttgtcgcc aggtgcacgg cgccgatgtg cgtgcggtcg ccgaacttca
     7561 gcgtcatcgc gatcaggttg acgacgattg tggcgcccag ttcgccggcg tgatggtagt
     7621 cagggttgcc gaggtcgccg atgaagaaac cgaagttcag cgtgcacgcc aggatgacga
     7681 acgcgccgaa gatgactttc tcgagattca tgtgctctcc ccgatggcgg ctcggcacca
     7741 ccgatcgacc gtaaggtatt gatcgatgcg ggccgccggc cgtatgttaa ttggggtctc
     7801 cgccggcgtc tcgaatcagt ggcaccgcgt caggaacctc ggtcaggaga ttgccgtctg
     7861 cgctgcacct ggaacgcacc gaagcccagg cacgccactg ctggccagaa cgtgacgaag
     7921 aacaacagtc cgaggccgat cggattgggg ttggggtccg gccacagccc gatggccgcc
     7981 aggaggatga cagccagcag cggaccccaa ccgaggagga cgaggacaag tccgatcttg
     8041 acccagcggt tggcgatgta gtccttcagc cggacgcaca ccgcaacgac gggcttcaga
     8101 gcctaggcct tgcggtagat ctcgccgccc ttctgcacga actcgaccgc cttctcctgc
     8161 atgcccttgt tcagcgcctg ctccgcgctg acgccctgct tcgccgcata gtcgcgcacg
     8221 tcctgcgtga tcttcatgct gcagaagtgc ggaccacaca tcgagcagaa gtgcgcggac
     8281 ttcatcgcgt ccttgggcag cgtctcgtcg tggaaatcct tcgcggtgtc cgggtcgagt
     8341 ccgagattga actgatcttc ccagcgaaac tcgaagcgcg cctttgaaag cgcgttgtcg
     8401 cgcatctgcg cgcccggatg gcccttggcg aggtcggctg catgcgcggc gatcttgtag
     8461 gccatgatgc cgtccttgac gtccttcttg ttcggcagcc cgaggtgttc cttgggggtg
     8521 acgtagcaga gcatcgcggt gccgtaccag ccgatcatcg ccgcgccgat cgcgctcgtg
     8581 atgtggtcgt agcccggcgc gatgtcggta gtcaggggtc cgagcgtata gaacggcgcc
     8641 tcgtggcaca gccgcagctg ctcatccatg ttgtgcttga tcatgtgcat cggcacatgg
     8701 cccggcccct cgatcatcac ttgcacgtcg tgcttccagg caacctgcgt gagttcgccg
     8761 agcgtggcga gctcggccat ctgtgcctcg tcgttggcgt cgtagatcga gccgggccgc
     8821 aggccgtcgc cgagcgagaa ggcgacgtcg taggccttca tcagttcgca catctcttcg
     8881 aagtgcgtgt aaagaaaact ctccttgtga tgcgacaggc accacttggc catgatcgag
     8941 ccgccgcgcg agacgatgcc ggtcatgcgc ttcgccgtca tcgggatgta cggcaggcgc
     9001 acgccggcgt ggatcgtgaa gtagtccaca ccctgctccg cctgctcgat cagcgtgtcg
     9061 cggaagatct cccaggtcag atcctcagcc ttgccatcga ccttttcgag cgcctggtaa
     9121 atcggcacgg tgccgatcgg caccgggctg ttacgcagga tccactcgcg cgtctcgtgg
     9181 atgtgcttgc cggtggaaag atccatcacg gtgtccgctc cccagcggat cgcccaggtc
     9241 atcttttcga cctcttcgcc gatcgaactc gtcaccgccg agttaccgat gttggcgttg
     9301 atcttcacca ggaagttgcg gccgatgatc atcggctcca actcggcgtg attgatgttg
     9361 caggggatga tggcgcggcc tcgggcgatc tcgtcacgca cgaactcggg cgtga
//
LOCUS       DATIRT010000454         4583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1453881, whole genome shotgun sequence.
ACCESSION   DATIRT010000454 DATIRT010000000
VERSION     DATIRT010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453881"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VLN25_10280"
     CDS             <1..798
                     /locus_tag="VLN25_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10983.1"
                     /translation="GDVIRKLVVKAERKGLGFRYEDTEVQGMGLIGDASRLRAVASEL
                     IDNVIRNAPSGEIIVHFSALSTDERSIELAVSVTATAMTDEQSTLVGRPLNLPAKAAG
                     RSKPRAGRDAQRRTIDVAGVETQVVATPDRGAVFKARMHYAVDLSRVAIDLGALAGVS
                     APASDSSDADPRLLAEYARASARLRHNADKGNLGALWAQAHRLKEIWLPCAPLSEAGL
                     VSALAHTARGGDGANARMLARRLAEALDATVRQQMSASDLELEEPVA"
     gene            892..1425
                     /locus_tag="VLN25_10285"
     CDS             892..1425
                     /locus_tag="VLN25_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10984.1"
                     /translation="MNRILTLGAALLLAGCTALGPPAIVLSRGDIAERAFIDRASIDP
                     KKIFKGMQGLDIAGPDIGFQTTAQRIELAWTAKLADGPIGIPLSLRISMSGAPVLNAQ
                     RNGIDLADARIEEIRLPSVPFINLDTRQMNQGGDSLGTLPLLQFRPEELNRDGIVYQA
                     TGISLGTFGLRVDLAPK"
     gene            1441..2298
                     /locus_tag="VLN25_10290"
     CDS             1441..2298
                     /locus_tag="VLN25_10290"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HSQ10985.1"
                     /translation="MDLIVLVKAAILGIVEGLTEFLPISSTGHLIVTSSLLEFRGAQV
                     ATFEIAIQTGAMFAVIWEYRRRLLATLAGITSQAVAQRFVINVAVAFAPAVVFGLLLG
                     KVIKEYLFHPVPVAAAFVVGGVVILWAEARHHRLYGERDLLGQRHARVETVDDMTPLD
                     ALKVGLMQCIALIPGTSRSGATIIGAMILGLSRKAATEFSFYLAIPTLIGAGAYSVWK
                     QREAFVAADAPVFAVGLFFAFVSAWLCIRWLIRFISTHDFTVFGWYRIAFGGVILLTA
                     WLGWVDWAR"
     gene            2366..3898
                     /locus_tag="VLN25_10295"
     CDS             2366..3898
                     /locus_tag="VLN25_10295"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSQ10986.1"
                     /translation="MNRRDECQELCALLRSRFPIILIQSHEEPRILELLQQSANLENQ
                     VLMTWSITNGIRRHGRDEAIYQTNDLLDALKHIDKTAQNGIYVLCDAHPGFKDPVAMR
                     LTREIALSHYRSARTLVFLSPRLDEMPPELLRLSAHFHPRLPNRDDIRALVAEEARRY
                     ESQSGEKPRGDKQALEMLIMHLLGMEQDDVRRLVRQALRDDGTISTDDVRRVLATKYE
                     ALGGAGTLAYEESTVRFDDVGGLARLKHWISLRRKPFLDSGADEVDRPKGIVLLGVQG
                     GGKSLAARAVAGEWGVPLMRMDFGALYNKYYGETERNLRNAFAAAESMAPCVLWIDEI
                     EKGLSTDSGEGDGGVSRRVLGSLLTWMAERREPVFMVATSNDISQLPPELVRKGRFDE
                     IFFVDFPGAEARSQIARIHLKKRGHDPANFDVAGFAQLAEGFSGAEIEQAIVSASFEV
                     RSRSEPLTSADILAELERTRPLSVVMAEKIDKLRDWASERAVYADESTEESIKPVARR
                     AL"
     gene            4212..>4583
                     /locus_tag="VLN25_10300"
     CDS             4212..>4583
                     /locus_tag="VLN25_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose 1,5-bisphosphate carboxylase"
                     /protein_id="HSQ10987.1"
                     /translation="MNPDAVRAIYRLRATGPAAEKRAHALALEQSIEMPSEAVTDARV
                     LRDVVARVESLVAQPDGSTLATLLLSGESIAGDAGQLMNMLFGNSSLLDDVEVVAVAV
                     PDALAVRFGGPALGIDGLRRLT"
BASE COUNT          763 a         1494 c         1540 g          786 t
ORIGIN      
        1 ggcgatgtga tccgcaagct cgtcgtcaag gccgagcgca agggcctggg cttccgctac
       61 gaagataccg aggtccaggg catgggcctg atcggcgatg cgtcgcgcct gcgcgcggtc
      121 gcctccgaac tcatcgacaa cgtgatccgt aacgccccga gcggcgagat catcgtgcac
      181 ttttccgcgt tgtcgaccga tgagcgcagc atcgaactcg cggtcagcgt gaccgcgacc
      241 gccatgacgg acgagcagag cacgcttgtc ggacggccac tcaacctgcc ggccaaggcc
      301 gctggccggt cgaagccacg tgccgggcgt gacgcgcagc gacgcacgat cgacgtcgcg
      361 ggcgtagaga cgcaggtggt tgcgactccg gatcgcgggg ccgtgttcaa ggcacgcatg
      421 cattacgcgg ttgacctctc gcgcgtggcc atcgatctcg gcgcgctggc cggggtgtcg
      481 gcgcctgcct ccgactcgtc ggatgcggat ccgcgcctgc tggcggagta cgcacgggcc
      541 tcagcgcgcc tgcgacacaa cgcggataag ggcaacctgg gtgcgctctg ggcacaggcc
      601 caccgcctga aggagatctg gctgccgtgc gcgcccttgt ccgaggccgg actggtcagc
      661 gctttggcgc atacggcgcg cggcggcgat ggcgcgaacg cgcgcatgct ggcgcgtcgt
      721 ctggccgagg cgctcgacgc aaccgtgcgc cagcagatga gcgcatccga tctcgaactc
      781 gaagaaccgg tggcctgaga ggctcccgcg tgcctgcgac gacgcacgct accattgcgc
      841 gccgcgctgt tccgcgaaga tccgccggaa cgcgtcgttc acggaaaccc catgaatcgc
      901 atcctcacac taggtgccgc actgctgctt gcggggtgca cggcgctcgg acctccggcc
      961 atcgtgctga gccgcggcga catcgcggaa cgtgccttca tcgatcgcgc gagcatcgac
     1021 ccgaagaaga tcttcaaggg catgcagggc ctggacattg ccggaccgga catcggtttc
     1081 cagaccacgg cgcagcgaat cgagctggcg tggaccgcga aactcgccga tggaccgatc
     1141 ggcatcccgc tgtcgctgcg catatccatg tccggcgccc ccgtcctcaa cgcgcagcgc
     1201 aatggcatcg acctggccga cgcccggatc gaggagatac gtctgccgtc ggtgccgttc
     1261 atcaatctcg acacgcggca gatgaaccag gggggagatt cgctgggaac ccttcccttg
     1321 ctgcagttcc ggccggagga actgaaccgc gatggcatcg tctaccaggc cacgggcatc
     1381 tcgctcggca cgttcggcct gcgcgtcgac ctcgctccca agtaaaccgg gagtctgcat
     1441 ttggatctga tcgtgctggt caaggcggcc attctcggca tcgtcgaggg actcaccgag
     1501 ttcctgccga tttcatcgac cggacacctg atcgtcacca gttcgctgct cgaattccgc
     1561 ggtgcccagg tggccacgtt cgagatcgcg atccagaccg gtgcgatgtt cgccgtgatc
     1621 tgggagtatc gccgtcgact gttggcgacg ctcgctggca tcacgtcgca agcggtcgcg
     1681 cagcgtttcg tcatcaacgt cgccgtggcg ttcgcgccag cggtggtctt cggccttctg
     1741 ctcggcaagg tgatcaagga gtaccttttc cacccggtgc cggtggccgc cgccttcgtc
     1801 gttggcggtg tcgtgatcct gtgggccgag gcgcgccacc accgcctgta tggcgagcgc
     1861 gacctgctcg gccagcgtca tgcgcgggtc gagacagtgg atgacatgac gccgcttgac
     1921 gcgctcaagg tcggccttat gcagtgcatt gcgctgatac cgggcaccag ccgctccggc
     1981 gcgaccatca tcggcgcgat gatcctcggg ctgtcgcgca aggcggccac cgagttcagc
     2041 ttctatctcg cgataccgac gctgatcgga gccggtgcgt actcggtctg gaagcagcgc
     2101 gaggcgttcg ttgccgccga cgccccggtc tttgcggtgg gattgttctt cgcgttcgtg
     2161 tcggcgtggc tgtgcatccg ctggctgatc cgcttcatct ccacgcacga cttcacggtg
     2221 ttcggttggt accggatcgc cttcggcgga gtgatcctgc tcaccgcgtg gcttggctgg
     2281 gtcgactggg cgcgctgatg gacgtcgccg agccgcgacc gatcgaacac cggctggatg
     2341 cggtgatcga ccggaggatt tcgcagtgaa tcgcagggac gagtgccagg aactgtgtgc
     2401 gctgctgcgc tcgcgcttcc cgatcatcct gatccagtcg cacgaggagc cgcgcatcct
     2461 cgagttgctg cagcagtcgg ccaacctcga gaaccaggta ctgatgacct ggagcatcac
     2521 caacggcatc cgtcgccatg gccgcgacga ggcgatctac cagaccaacg acctgctcga
     2581 tgcgctcaag cacatcgaca agactgcaca gaatggcatc tacgtgctgt gcgacgccca
     2641 tccgggattc aaggatccgg tcgcgatgag gctgacgcgc gagatcgccc tgtcgcacta
     2701 caggagcgcg cgtacgctgg tcttcctgag tccgcgcctg gacgagatgc cgccggaact
     2761 gctgcggctg tccgcgcact ttcatccgcg tctgccgaat cgggacgaca tccgcgcact
     2821 cgtcgccgag gaggcacggc gttacgagtc gcaatcgggc gagaagccgc gcggcgacaa
     2881 gcaggcgctc gagatgctga tcatgcatct gctgggcatg gagcaggacg acgtacgccg
     2941 gcttgtgcgg caggccctgc gcgatgacgg caccatcagc accgacgatg tgcggcgtgt
     3001 gctggcgacg aagtacgaag ctctcggtgg cgccggcacc ctcgcgtatg aggaaagcac
     3061 ggtccgcttt gacgatgtcg gcggactggc gcgcctgaag cactggatat cgcttcgacg
     3121 caagccgttc ctcgacagcg gagccgatga agtcgaccgc cccaagggca tcgtgctgct
     3181 cggcgtccag ggcggcggca agagcctggc cgcgcgtgcg gtggcaggcg agtggggcgt
     3241 gccgctgatg cgcatggact tcggtgcgct ttacaacaag tactacggcg agaccgagcg
     3301 caacctgcgc aatgcgttcg ccgctgccga gagcatggcg ccctgcgtgc tctggatcga
     3361 cgagattgaa aagggcctct cgacggacag cggcgaaggc gacggcggcg tatcacggcg
     3421 cgtgctcgga tcgctgctga cgtggatggc cgagcgcagg gaaccggtgt tcatggtggc
     3481 cacctccaac gacatctcgc agctgccgcc ggaactggtg cgaaagggcc gcttcgacga
     3541 gatcttcttt gtcgacttcc cgggcgcgga ggcacggtcg cagatcgcgc gcatccatct
     3601 gaagaaacgc ggtcacgatc cggcgaactt cgacgtcgcc ggcttcgcgc aactggccga
     3661 gggattttcc ggcgccgaga tcgagcaggc gatcgtatcg gcctcgttcg aggtgcgctc
     3721 ccgcagtgag ccgctgacct cggccgacat tctcgccgaa ctcgagcgca cgcgaccgct
     3781 gagcgtggtg atggcagaga agatcgacaa gttgcgcgac tgggcgtccg agcgcgcggt
     3841 gtatgcggac gaatccaccg aggaatctat caagcccgtt gcgcggcgcg cgctgtaacg
     3901 cggtccgtgc catccttcgt acggacggtg cgtcacctgt caacgctact cgtcgcctgt
     3961 gccactcacg caacaggggg gtggacggac ccggcgcatt ccgcgagagt tcccctcagg
     4021 tcgggttcgc ctggccgccg aaggcgctct gcgccgtcgg gcagaaagcg ggaggcgagc
     4081 gcagccgctt ggccgggcgg ctacgctcgc ccaccccgtg aactgcatac cgtcacacga
     4141 tcctgccgct gccgcaacgg gatctgtcga gacgcgtggg ggtcaattcc ccacgtttgt
     4201 agaatcgcgg catgaaccct gacgcagtgc gcgcaatcta tcgcctgcgc gcgactggcc
     4261 cggccgccga gaaacgcgcg catgcactgg cactcgagca gagcatcgag atgccgtccg
     4321 aggcggtgac cgatgctcgc gtgctgcgcg acgtcgtggc ccgtgtcgaa agtctcgtgg
     4381 cgcaacccga tggcagcacg cttgcaacac tcctgctcag cggcgagagc atcgccggcg
     4441 atgccgggca gctgatgaac atgctgttcg gcaacagctc cctgctcgat gacgtcgaag
     4501 tggtggcggt ggccgtgccg gacgcgctgg cggtccgatt tggcgggcca gcgctcggta
     4561 tcgatggcct gcgccgactg acc
//
LOCUS       DATIRT010000455         2544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_864091, whole genome shotgun sequence.
ACCESSION   DATIRT010000455 DATIRT010000000
VERSION     DATIRT010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864091"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1387
                     /locus_tag="VLN25_10305"
     CDS             <1..1387
                     /locus_tag="VLN25_10305"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HSQ10988.1"
                     /translation="ARFAIANPADGVKIAEVANCDRVDAEQAISAADRAWPAWRRKTD
                     KERAAVLRTWFDLILAHQEDLAKLMTAEQGKPLAESRGEVIYGASFIEWFAEEAKRIY
                     GDTIPTVANDRRLFVFKEPIGVAAAVTPWNFPNAMITRKVAPALAAGCTVVVKPAEQT
                     PLSALALAELAARAGFPPGVFNVVPADEMRSIEVGKALCESPVVRKLSFTGSTAVGRI
                     LMQQSAPTLKKLSLELGGNAPFIVFDDADLDAAVDGALASKYRNAGQTCVCTNRFYAQ
                     AGIYDAFVEKLAAKAQQLKVGSGFDDGVQQGPLIDRNALAKVEAHVADARAKGARVLT
                     GGAPHELGGTFFQPTVLADVTNDMLVAREETFGPVAPVFRFETEDQAVAAANATEFGL
                     AAYFYSRDVARVFRVAERLESGMVGVNTGLISNEVAPFGGVKQSGIGREGSKYGIDEY
                     LVVKYVCIGGI"
     gene            1387..1770
                     /locus_tag="VLN25_10310"
                     /pseudo
     CDS             1387..1770
                     /locus_tag="VLN25_10310"
                     /inference="COORDINATES: protein motif:HMM:NF018595.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
     gene            1873..2031
                     /locus_tag="VLN25_10315"
     CDS             1873..2031
                     /locus_tag="VLN25_10315"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HSQ10989.1"
                     /translation="MNGPLLVEGTVEFHAADGTIFVTDKVWLCRCGHSSNKPFCDGTH
                     KRVGFTSV"
     gene            2266..>2544
                     /locus_tag="VLN25_10320"
     CDS             2266..>2544
                     /locus_tag="VLN25_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ10990.1"
                     /translation="MNAASPLRAALLASMFRPGQWIGPAAVALSTIALALLCVPHPVA
                     WATLFGWLGWIVFALSLVLTVRARALAAHFGGLDHQYAWHHVLGLLAYV"
BASE COUNT          420 a          834 c          836 g          454 t
ORIGIN      
        1 tgcgcgcttt gctatcgcca atccggcgga cggcgtgaaa attgccgaag ttgccaactg
       61 cgatcgcgtg gacgccgagc aggcgatctc cgccgccgac cgggcctggc ccgcatggcg
      121 acgcaagacc gacaaggagc gcgcagccgt attgcgcacg tggttcgacc tgatcctcgc
      181 gcaccaggag gacctggcca agctgatgac tgccgagcag ggcaagccgt tggccgagag
      241 ccgcggcgaa gttatctacg gagcttcgtt catcgagtgg tttgccgaag aggcaaagcg
      301 gatctacggc gacacgatcc cgaccgtcgc caatgatcga cgcctgtttg tgttcaagga
      361 gccgatcggc gttgccgctg cggtcacgcc ttggaatttc ccgaacgcga tgatcacgcg
      421 caaggttgct ccggcacttg cggcgggttg cactgtggtc gtcaagccgg ccgagcagac
      481 tccgctgtcg gcactggccc tggccgaact ggcggcgcgc gccggtttcc cgcccggagt
      541 gttcaacgtc gtgcccgccg acgagatgcg ctcgatcgag gtcggcaagg ccctgtgcga
      601 atcaccggtc gtgcgcaagc tctctttcac cgggtcgacc gccgtcggtc gcatcctgat
      661 gcagcagagc gccccgacgc tgaagaagct gtccctcgag ctcggcggca atgcgccgtt
      721 cattgtcttt gacgacgccg acctcgacgc cgcagtcgac ggcgcactcg cctcaaagta
      781 ccgcaacgcc ggccagacct gcgtgtgcac gaatcgcttt tacgcacagg ccggcattta
      841 cgacgccttc gtcgagaaac tcgccgccaa ggcccagcag ctcaaggtcg gctcgggctt
      901 cgacgacggc gtgcagcaag ggccgttgat cgatcgcaac gcgctcgcca aggtcgaggc
      961 gcatgtggcc gatgcgcgcg cgaaaggcgc acgcgtactc accggtggcg cgccgcatga
     1021 actcggcgga acgtttttcc agccgaccgt gctcgccgac gtgacgaacg acatgctggt
     1081 cgcacgtgag gaaacctttg gcccggtggc gccggtgttc cggttcgaga ccgaggacca
     1141 ggccgtcgct gccgccaacg cgaccgagtt cgggctggcc gcgtatttct actcgcggga
     1201 cgtggcgcgg gtgttccgcg tcgccgaacg cctcgaaagc ggcatggtcg gcgtcaacac
     1261 cggcctgatc tcgaatgaag tcgcaccctt cggtggcgtc aaacaatcgg gcatcggccg
     1321 cgaaggctcg aagtacggca tcgacgagta cctagtcgtg aagtacgtct gcatcggcgg
     1381 gatctgatgg cgatacgcac ctacacgggc aagggcgtca ctgtcgggta cgagctgaac
     1441 cgctgcatcc atgcggaaga gtgcgtacgc ggtgcaccgc aggtcttcga tcgggacgcc
     1501 aggccatgga ttcagcccga caatgcgagt gtcgagcacc tcgttgcggt cgtcgcgcgt
     1561 tgccccaccg gcgcattgac gctgcaggcg cccgatggtg gaacgctcga ggcgcctccc
     1621 gatcacaatg tcgcttcggt gcagccccgt ggcccgatct acctgcgcgg gcgcgtctca
     1681 gttcccggcg ttgatcacgc gaccctggtc gagtacacgc gtgtcgccct atgccgctgc
     1741 ggtgcctcgg cgaacaagcc cttctgcgat gggagccaca accggagcgg cttcgccgac
     1801 gccggtgcgt gcaccaacgc gcctgcgtcg gtggcttcga caccctcggg gtcggtgaag
     1861 atcaatccaa tcgtgaacgg tcccctgttg gtggaaggca ccgtggagtt ccacgcggcc
     1921 gacggcacga tctttgtgac ggacaaggtg tggctgtgtc gctgcggcca ctcctccaat
     1981 aagccgtttt gcgacggcac gcacaagcgg gtggggttca cgtcggtctg agcagacagt
     2041 ctgtatcggt atctgccgca agcacaggcc cggcctcgca tcagcacact atccggcgaa
     2101 ctctgcggct taagttcagc acaacgtgca cgattgcgcg ccgcgtcgcg cgcgtactcg
     2161 gcaacggtac cgatcgttga cgggcggcaa tgcagcgcgt cgggccgcgc cgcgtgcggc
     2221 cctgtcggtt gctagagtcg acacggaccc aaacgtgact tgccgatgaa cgccgcctct
     2281 cccttgcgcg ctgccttgct ggcgagcatg ttccgccccg ggcaatggat cggtccagca
     2341 gccgtggcgt tgtccactat cgcgctggcg ctgctgtgcg taccacaccc ggtggcgtgg
     2401 gccacgctat tcggctggct cggctggatc gtgtttgcgc tgtcgctggt gttgaccgtc
     2461 cgtgcacgcg cgctggcggc tcacttcggc gggctcgatc accagtatgc gtggcaccac
     2521 gtcctcgggc tactggccta cgtg
//
LOCUS       DATIRT010000456         4294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1552780, whole genome shotgun sequence.
ACCESSION   DATIRT010000456 DATIRT010000000
VERSION     DATIRT010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552780"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="VLN25_10325"
     CDS             <1..834
                     /locus_tag="VLN25_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSQ10991.1"
                     /translation="LLDQNREISDEPDARPLAVDRAEVRFEHVDFAYDPRRQVLHDVD
                     FTIVGGTTTAVVGATGSGKSTLARLLFRFYEVGDGAVLIDGQDVRKITQESLRAAIGI
                     VPQDTVLFNDTIEYNIAYGRPGAGRADVVTAARAAHIHDFIELLPDGYTTRVGERGLK
                     LSGGEKQRVASARTLLKNPAILVFDEATSALDSRTERVIQDELEGIARSRTTLVIAHR
                     LSTVVHADQILVMDHGRIVERGTHAELLEHRGAYAAMWDIQSRERARTEASTAEEARA
                     A"
     gene            1100..2134
                     /locus_tag="VLN25_10330"
     CDS             1100..2134
                     /locus_tag="VLN25_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HSQ10992.1"
                     /translation="MIKSSGRILAGGLVAAAALASAPAHAGKTLDAIKARGSVVCGVN
                     TGLAGFSAADSQGNWSGLDVDICRAVAAAVLGDGNKVKFVPLNAQQRFTALQSGEVDV
                     LSRNTTWTLTRDASLGMFFAPVTYYDGQGFMVPVKGKVKNAKDLKGATVCVQSGTTTE
                     KNLTDFSRANNLGIKPVVFEKVEAATGAYFTGRCQAYTTDASGLASVRNKEAKDPNEH
                     VILPTLISKEPLGPAVRRGDDEWYTIVKWVVFGLVEAEEYGLTQKNVEEMVKTSQDPV
                     VMRIVGSSEDTGKLMGLDKNWMVNALKATGNYGEIFERNVGPNSALKLPRGLNNQWNK
                     GGIMYAPPVR"
     gene            2246..3436
                     /locus_tag="VLN25_10335"
     CDS             2246..3436
                     /locus_tag="VLN25_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464611.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSQ10993.1"
                     /translation="MSQAVSGRAPPKRRDWSWRSQAFRGLVYQIIAIALVAFGVWFLA
                     HNTLENMRVRGIQSGFGFLTQPAGFDIGETLFNYDALDAYWIAFLVGIANTLRVAIIG
                     VVLTTVLGALLGIGRFSRNALVRGLCYGYVELFRNIPVLLQLLMWYLVFTELLPDPFD
                     PVSAFGLVFLSKGGFQYPVPVWGLGQGLAVVGALVGIGLAWLYRRWARQAFERTGHVR
                     SMFWVPLAIVIATAVVGWLVGGAPTAWNIPHHGEGMVEGGSAVTPEFMAVLLGLVFYT
                     SAFVAEVVRAGIQSVSHVQIEAASSLGLSRGHSMRLVILPQAMRVIIPPMTNQYLNLT
                     KNSSLAVAIGYPDVVSIANTAINQTGRAVECIAIIMAVYLTTSLTTSAIMNWYNKRSA
                     IKER"
     gene            3585..>4294
                     /locus_tag="VLN25_10340"
     CDS             3585..>4294
                     /locus_tag="VLN25_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464612.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSQ10994.1"
                     /translation="MSAVFQPVPPREAPVKQAGVVYWLRTNLFADWKSALTTVVLIGI
                     ALVYLPPLLDWAIVQAVLQPNADVCQQARGTGACWGVIVEKYRLIIFGRYPYEEQWRP
                     QIATGLLVGLLMASCVRMFWKPWLVVLWVAVLAAFFALMYGGVFGLSVVTTDRWGGLP
                     LTIMLASLSIVLAFPIAVLVALGRKSRLPAIRTVCIIYVELVRGVPLISVLFMASFMF
                     PLFMPQGFTIDVLVRVLVG"
BASE COUNT          742 a         1314 c         1469 g          769 t
ORIGIN      
        1 ctgctcgacc agaaccgcga gatttcggat gagccagatg cgcggccact ggcggtcgac
       61 cgcgccgagg tgcgcttcga gcatgtcgac ttcgcctacg acccccgccg gcaggtcctg
      121 cacgacgtcg atttcaccat cgtcggcggc acgacgacgg cggtggtcgg cgcgaccggc
      181 tcgggcaagt cgacgctggc acgactgctc ttccgcttct atgaagtggg cgacggtgca
      241 gtgctcatcg acggccagga tgtgcggaaa atcacgcagg aaagcctgag ggcggcgatc
      301 ggcatcgtgc cgcaggacac ggtgctgttc aacgacacga tcgagtacaa catcgcctac
      361 ggcaggccgg gcgccgggcg tgccgacgtg gtcacagcgg cccgggccgc gcacattcac
      421 gacttcatcg agttgctgcc cgacggctac acaacgcggg tgggtgagcg cgggctcaaa
      481 ctgtcgggag gcgagaagca gcgcgtggcc agtgcgcgca cgctgctgaa gaatccggcg
      541 atcctggtct tcgacgaggc gacgtcggcg ctcgattccc gtaccgaacg cgtgatccag
      601 gatgagctgg agggcatcgc acggagccga accacgctgg tcatcgcgca tcggctgtcg
      661 acggtcgtgc atgcagacca gatcctggtg atggaccacg gacgcatcgt cgagcgcggc
      721 acccatgccg aactgctgga gcaccggggt gcttacgcag cgatgtggga tatccagagc
      781 cgtgagcgcg ccaggaccga ggcgagtacg gcagaggaag cgcgagccgc ttgagggcgg
      841 cgcgggccgg cggcaagtcc gctcagcggg cggggcgact tccaggcaga agcggtcact
      901 ccaaggccac gcccgcgcag gcggcggttc acgggcgaag gcggcgccgc ccagcctaag
      961 tggcaacccg gacatggcac ggatggtgcg tacttgcccc gggtacgcta gattagctat
     1021 tcgggcgttc aagggcgcac gaaaatcgtg caacgcgcac acacccgcgg ggcgggttct
     1081 cgaaaaaagg agcaagggaa tgatcaagag ttcaggcagg atcctcgcgg gaggactggt
     1141 tgcggctgct gcgctggcgt cagctcccgc gcatgcgggc aagaccctcg atgccatcaa
     1201 ggcgcgcggc agcgtcgttt gcggcgtgaa taccggtctg gcgggcttct cggccgccga
     1261 cagccagggc aactggtccg gactggacgt cgatatctgc cgcgcggttg ccgcggcggt
     1321 gctcggcgac ggcaacaagg tcaagttcgt tccgctgaat gcgcagcagc gcttcacggc
     1381 gctgcagtcg ggcgaggtcg acgtcctgtc tcgcaacacc acgtggacac tgacgcgcga
     1441 cgcgtctctg ggaatgttct tcgcgcctgt gacctactac gacggccagg gcttcatggt
     1501 cccggtcaag ggcaaggtca agaacgccaa ggacctcaag ggcgctaccg tttgcgtaca
     1561 gtcgggcacg accactgaga aaaacctcac cgacttctca cgtgccaaca acctcggcat
     1621 caagccggtc gtgttcgaga aggtcgaggc ggccacaggg gcgtatttca ccggccgttg
     1681 ccaggcctat acgacggatg cctcgggact cgcctcggtt cgtaacaagg aagccaagga
     1741 tccgaacgag cacgtgatcc tgccgacgct gatctccaag gaacccctcg gacccgcggt
     1801 gcgccgcggc gatgacgagt ggtacacgat cgtaaagtgg gtcgtcttcg gcctggtcga
     1861 ggccgaggag tacggcctga cgcagaaaaa cgtcgaggaa atggtcaaga cgagccagga
     1921 cccggtggtc atgcggatcg tcggcagttc cgaggacacg ggcaagctga tgggcctcga
     1981 caagaactgg atggtcaatg cgctgaaagc caccggcaac tacggcgaga tcttcgagcg
     2041 caatgtcggt ccgaactcgg cgctcaagct gccgcgcgga ttgaacaacc agtggaacaa
     2101 gggcggcatc atgtacgcgc cgccggtgcg ttgatggctt gacctcaggc ctgtcgcgcg
     2161 agcggcaggc cgctaggcag agcggaggcg caagcgtccg ctccgcctag acgaagaacg
     2221 aaaacgttcc gagcaggagg agaccgtgtc gcaagccgta tccgggcgtg ctccgcccaa
     2281 gcggcgtgac tggtcctggc gcagccaggc cttccgcgga ctcgtctacc agatcatcgc
     2341 gatcgcgctg gtcgcgtttg gagtctggtt cctcgcccac aacacgctgg aaaacatgcg
     2401 tgttcggggg atccagagcg gcttcggctt cctgactcag ccggccggct tcgacatcgg
     2461 cgagacgctg ttcaactacg acgcgctcga tgcgtactgg atcgcattcc tcgtcggcat
     2521 cgccaacacg ttgcgggtcg ccatcatcgg cgtggtgctg acgacagtgc tcggcgcgct
     2581 gctcggcatc ggccggttct cgcgcaacgc gctcgtgcgc ggcctgtgct acggatatgt
     2641 ggagctgttc cgcaacatcc cggtgctgct gcaattgctg atgtggtacc tggtgttcac
     2701 cgaattgctg cctgatccgt tcgaccccgt gtccgcgttc gggctggtgt tcctcagcaa
     2761 gggcgggttc cagtacccgg ttccggtttg gggccttggc cagggattgg ctgtcgtcgg
     2821 cgcgctggtc ggtatcggac tggcatggct gtaccgccgc tgggcgaggc aggcattcga
     2881 gcggacgggt catgtgcgca gcatgttctg ggtgccgctg gcgatcgtca ttgcgacagc
     2941 ggtcgtcggc tggctcgtcg gcggggcacc gaccgcgtgg aacattccgc accatggtga
     3001 aggcatggtc gaaggcggat cagcggtgac gccggagttc atggcggtgc tgctgggctt
     3061 ggttttctat acgtcggcat tcgtcgccga ggtcgtgcgg gccggcatcc agtcggtctc
     3121 gcacgtacag atcgaagcgg ctagcagtct gggactgtcg cgcggccact ccatgcgcct
     3181 ggtgatcctg ccgcaggcga tgcgcgtgat cattccgccg atgacaaacc agtacctgaa
     3241 cctgacgaag aattcgtcgc tggcggtggc gatcggctac ccggacgtcg tttcgatcgc
     3301 gaacaccgcg atcaaccaga ccggccgcgc ggtcgagtgc atcgcgatca tcatggccgt
     3361 gtacctgacc acgtcgctca ccacgtcggc catcatgaat tggtacaaca aacgctcagc
     3421 gatcaaggaa agatgatgac gggtacggcc gctctctcat tgatcgttgc gtcgcctttg
     3481 tattcgcggc gggcgcccgg ccgcccgaag cccgccgcgc aattcgaact cgatgaaggg
     3541 cacgtgcgcc gctccgcggc gcgtacgctt catcgaactg gcctatgagc gccgtcttcc
     3601 aacccgtgcc gccgcgcgag gcgccggtga agcaggccgg cgtcgtctat tggctgcgca
     3661 cgaatctgtt tgccgactgg aaaagtgcgc tgacgacggt tgtcctgatc ggaatcgcgc
     3721 tcgtctacct gccgccgctc ctcgactggg cgatcgtgca ggcggtgctg caacccaacg
     3781 ccgacgtctg ccagcaggca cgcggcacgg gtgcctgctg gggcgtgatc gtcgagaagt
     3841 accggctgat catctttggc cgctatccgt acgaagaaca gtggcggcca cagatcgcca
     3901 ctggactgct ggtcgggctg ctgatggcga gctgcgtccg catgttctgg aagccctggc
     3961 tcgtcgtgct gtgggtggct gtcctggcgg cgtttttcgc gctgatgtac ggcggcgtgt
     4021 tcggattgtc tgtggtcaca acggatcgct ggggcggcct accgctgacg atcatgctgg
     4081 cgtcgctgtc gatcgtgctg gcgttcccga ttgcggtgct ggtggcgctc ggccgcaagt
     4141 caaggctgcc ggcgatccgg accgtctgca tcatctacgt cgagctggtg cgcggcgtgc
     4201 cgctgatctc ggtgctgttc atggcgagct tcatgtttcc gctgttcatg ccgcagggat
     4261 tcacgatcga cgtgctggtg cgagtgctgg tggg
//
LOCUS       DATIRT010000457         1894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_962310, whole genome shotgun sequence.
ACCESSION   DATIRT010000457 DATIRT010000000
VERSION     DATIRT010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962310"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1894
                     /gene="lon"
                     /locus_tag="VLN25_10345"
                     /pseudo
     CDS             1..1894
                     /gene="lon"
                     /locus_tag="VLN25_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585905.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase La"
BASE COUNT          423 a          570 c          611 g          290 t
ORIGIN      
        1 cgtgttggcc cagttcgata aatacgtcaa actcaacaag aagatcccgc ccgaggtgct
       61 gacctccctg gccgggatcg agaaagccgg gcgtctggcc gataccatcg ccgcccatct
      121 gccactgaaa ctcgaacaga aacaggaagt gctggaagtt ttcggcgttt cggagcagct
      181 ggaaaaactg ctcggccagt tggaagccga actcgatatc ctgcaggtcg aaaagcgcat
      241 ccgcggccgc gtcaagcggc agatggagaa gagccagcgc gagtactacc tgtacgagca
      301 ggtcaaggct atccagaagg aactgggcga gggcgaggaa ggtgccgacc tcgaggacct
      361 cgagaaaaag atccgcgccg cccgcatgcc caaggccgca cgcgacaagg cgaactcgga
      421 gctgaagaag ctcaagctga tgtcgccgat gtcagccgaa gccaccgtcg tgcgcaacta
      481 cctggacacg ctcgtggggc tgccgtggaa gaagcgcagc cgcatcaaca acgacctgac
      541 gaacgccgaa aaggtcctga acgacgatca ttacggactc gagaaggtca aggaccgcat
      601 cctcgagtac ctcgcggtgc agcagcgcgt cgacaaggtg aaggcgccga tcctgtgcct
      661 ggtcggccct ccgggagtag gcaagacctc cctcggccag tcgatcgcgc gcgcgacgaa
      721 tcgcaagttc atccgcatgg agttgggcgg cgttcgcgac gaggccgaga tccgcggcca
      781 tcgccggacc taaatcggct cgatgccggg caagatcctg cagaacctcg gcaaggccgg
      841 cgtgcgcaat ccgctcttcc tgctcgacga ggtcgacaag atgggcatgg acttccgcgg
      901 tgatccggct tcggcgctgc tcgaagtgct cgaccccgag cagaaccaca cgttccagga
      961 tcactacgtc gaggtcgact tcgatctctc ggacgtgatg tttgtcgcca ccagcaacac
     1021 gatgaacatc ccgccggcgc tgctcgaccg gatggaagtc atccgcctgt ccggctatac
     1081 ggaagaggaa aaggttcaca tcgcgctgca gcacctgctg ccgaagcaga tgaagaccaa
     1141 cgggctgaag tccggtgaac tgggagtggc ggaaagcgct atacgcgaca tcattcgcta
     1201 ctacacgcgc gaggccggcg tgcgctcgct cgagcgcgag atcagcaaga tctgccgcaa
     1261 ggtggtcaag cagttgttgc tgaagaagca ggaccacaag gtccaggtgg cggccaagaa
     1321 cctcgacaag ttcctcggcg tgcgccgcta caacttcggc gttgccgaga agcagaacca
     1381 ggtgggccag gtcaccggac tggcatggac cgaagtcggt ggcgacctgc tgacgatcga
     1441 ggtggcgacg atgccgggca aaggtaacgt gcagcgcacg ggctcgatcg gtgatgtcat
     1501 gaaggaatcg gtcgaggccg cgcgctcggt cgtgcgcgct cgggcacgcc ggctcggcct
     1561 gcgcgacgag atgttcgaga agatcgacct gcacgtgcac ttccccgagg gcgccacacc
     1621 caaagatggc ccgtcggccg gcattgcaat cacgacctcg atcgtgtcag ccctgagcgg
     1681 gattcccgtg cgggctgacg tcgcgatgac gggtgagatc acgctccgcg gcgaggtgct
     1741 cgccatcggc ggcctgaagg aaaagctgct cgcagcccat cgcggcggca tcaagacggt
     1801 gttgatcccg gaggaaaacg tcaaggatct cgcggagatc ccggacaacg tgaagaaccg
     1861 gcttgagatc gtccctgtca agtggatcga caag
//
LOCUS       DATIRT010000458         5241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1944643, whole genome shotgun sequence.
ACCESSION   DATIRT010000458 DATIRT010000000
VERSION     DATIRT010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1944643"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="VLN25_10350"
     CDS             <1..827
                     /locus_tag="VLN25_10350"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HSQ10995.1"
                     /translation="PIQGADLPLDHGDRIAFGGRHLEVRATPGHTEGCMTLVLEGERL
                     AFTGDTLLIRGAGRCDFQHGSAHALYRSIVQEIFTLPDACLLFPAHDYSGRTVSSVVE
                     ERTHNPRIGGGADERDFVGFMENLNLPHPKQIDIAVPANLRSGQPADGKVPRPADWGP
                     VRQTYAGLLEVEPEWVAEHLADVHVLDVRQPQEMQDSLGRIASSQLIPLNELKSRVSE
                     IPRERPVVAVCHAGVRSGQATVILRSAGFDRCANLRGGMLLWSQLGLPTNRKGVGS"
     gene            complement(843..1049)
                     /locus_tag="VLN25_10355"
     CDS             complement(843..1049)
                     /locus_tag="VLN25_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6494 family protein"
                     /protein_id="HSQ10996.1"
                     /translation="MDEETFNLSIRKFLKMVGVSSQREIEQAVAKAIAARTISGTESF
                     PAKVTLEIAGLNLNVNFDGEIRLQ"
     gene            complement(1237..1752)
                     /locus_tag="VLN25_10360"
     CDS             complement(1237..1752)
                     /locus_tag="VLN25_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSQ10997.1"
                     /translation="MRPAAHAIAAIVLAMCVSSSATYAQTIAPLVVTASDKAIQWGPC
                     PAFLPTGCQIGVLHGDPAKPNVDIFFKVPGKSKIAAHKHTSAERMVLVQGEMQVTYEG
                     HKPVTLKRGSYAYGPAELVHEAACLSVEPCVLFIAFEQPLDAIPMKMAAAKAAEAKAT
                     TTEPKKKGGGC"
     gene            complement(1829..3025)
                     /locus_tag="VLN25_10365"
     CDS             complement(1829..3025)
                     /locus_tag="VLN25_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HSQ10998.1"
                     /translation="MLRSLSPCFSWIFITSLQLKSRLPRVGTTIFTVMSQLALKHGAV
                     NLGQGFPDFNCDPVLQRHLEDAVRAGHNQYAPMAGVPTLRAAIAAKNLTLYGHRYDAD
                     TEITITAGATQAIMASVLALVHPGDEVIVLEPTYDSYVPSIELAGGRPVYVPLDAENG
                     YAPDWDRIRAAITPRTRMLMLNFPHNPTGRVLRSDDLVALENIVESTGVMVLSDEVYE
                     HIVFDSVAHQSLMRSPALAERTVVISSFGKTFHTTGWKVGYACAPAAISAEIRKVHQF
                     MVFAVNTPAQHAFAAYLQDPSPYLDLPAFYQAKRNLLIDGLRRTPFRVLPCEGTYFVL
                     ADYSALSPAPEPEFAKTMIVKYGVAVIPVSVFYDAPHDRKVVRFCFAKNDSTLRDALQ
                     RISASL"
     gene            3055..4188
                     /locus_tag="VLN25_10370"
     CDS             3055..4188
                     /locus_tag="VLN25_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HSQ10999.1"
                     /translation="MTIDISSTFDSGAIDVVATAEDSAELRIRHDLRSDGSRAEFLQW
                     FHFRVSGADGSGAILRVTNAGESTYPGGWQDYRAVASVDRRRWERVPTHFDGKVLTIE
                     LPPAPATYVAYFEPFSWDRHLALLADCVARGARQRRLGSSLQGRDLDELMIGQGARPI
                     WIIARQHPGETMAEWFVEGLMQRLLDPADAVARELRQMATFHIVPNMNPDGSVLGNLR
                     TNAAGANLNREWLEPTPERSPEVQVVRDAIHASGCASFFDIHGDENLPYVFVAGCEML
                     PGFSDRQAAEQAAFSADFRTASPDFQSEHGYAAGKYKEDVLKLASKYVGHTFGCLSLT
                     LEMPFKDNANAPDEQTGWSAARSARLGADMLGPIRQHLMRTSA"
     gene            4185..5240
                     /locus_tag="VLN25_10375"
     CDS             4185..5240
                     /locus_tag="VLN25_10375"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSQ11000.1"
                     /translation="MTTIDGPKVTLKRAHANDRARVYEWLIGSDLTQNMMGGAMFPDH
                     PIPSLDAFTADYAEHYFDGTRPYAGRTLIISANGEDIGCVSHGPIDVLNDVVELDIWL
                     AERRAAGQGYGSEALVVLCDWLQSNYGVNRFLVRPSRRNVKALRAMRRAGFRETDLPA
                     RDVESKLSLRPGHYADELLLFRILPVPSSVFRPDPTQTYVFIDSEFTSLESPQLISVG
                     AVATDSTAFYAELAGWDPRTASDFVNDTVIPLLDGDAVPLSVGAEALGEWLGERARRG
                     ATTIISDSGFDRWALAELFGGENLPANVRWQRVPVAYEALDEATQRLNLRRHHALDDA
                     RALRQLVLEAQVPARPA"
BASE COUNT          921 a         1669 c         1733 g          918 t
ORIGIN      
        1 cgccgatcca gggcgcagac ctgccgctcg atcacggcga tcgcattgcg tttggcggcc
       61 gccacctgga ggtgcgcgcc acgccgggcc acaccgaagg ctgcatgacg ctggtgctcg
      121 aaggcgagcg cctggccttc accggcgaca cgctgctgat ccgcggcgcc ggccgctgcg
      181 acttccagca cggcagtgcc cacgctctgt atcgctcgat cgtgcaggag atcttcaccc
      241 tgcccgatgc gtgcctgctg tttcctgcgc atgactacag cggccgcacg gtgtcgtcgg
      301 tggtggagga gcgaactcac aacccgcgca ttggcggcgg cgccgatgaa cgcgacttcg
      361 tcgggttcat ggagaacctg aacctgcctc accccaagca gatcgacatt gcggtgcctg
      421 ccaatctgcg ctccggccag cccgccgatg gcaaggtgcc gcggccggcg gactggggac
      481 cggtgcgcca gacctatgcc ggcctgctcg aggtcgagcc cgagtgggtc gccgagcacc
      541 tagcagatgt acacgtgctc gacgtgcgcc agccccagga gatgcaggac tcgctcggac
      601 gcattgccag ctcgcagctc attccactga acgagctcaa gtctcgcgtg tccgagatcc
      661 cgcgcgaacg gccggtggtt gcggtctgcc acgccggagt gcgatcagga caagcgaccg
      721 tgatccttcg cagcgccgga ttcgatcgct gcgccaacct gcgcggcggc atgctgttgt
      781 ggtcacagct tgggctgcct actaaccgca agggcgtggg ctcgtaggcc cggttagcgc
      841 gcttactgaa gccggatctc gccgtcaaag ttgacgttca ggttcaggcc agcgatttcg
      901 agggttacct tggccggaaa cgattccgtc cccgaaatgg tgcgagccgc aatcgccttc
      961 gccaccgcct gttcgatttc gcgctgcgaa ctgacgccca ccatcttcag gaacttgcga
     1021 atgctcagat tgaatgtttc ctcgtccatg gagttctccc gttgaatgat gaatgaaggc
     1081 gctgtcgaca gccggcatcg agtatgagcg tccgaagtcc gaagtccgaa gtccgaagtc
     1141 cgaagtccga tatccggcgt ccggttccgg tgacgccatt gctctgcacg ctcgcagact
     1201 ttgcccggca ggctgcctcg gggatggcca ggactcctaa cagccgccgc cctttttctt
     1261 cggctccgtg gtcgtcgcct ttgcctcggc tgctttggcg gccgccattt tcatcgggat
     1321 agcatcgagc ggctgttcga acgcgatgaa caacacacag ggctcgacgc tgaggcaggc
     1381 cgcctcgtgc accagttcgg ccgggccgta ggcatacgag ccgcgtttca gcgtgaccgg
     1441 cttgtggccc tcgtaggtca cctgcatctc gccctgcacc agcaccatgc gctcggcgga
     1501 cgtgtgcttg tgcgcggcga tcttggactt gcccggcacc ttgaagaaga tatcgacgtt
     1561 gggcttggcc ggatcgccat gcagcacgcc gatctgacac cctgtgggga gaaaggcggg
     1621 gcagggaccc cactggatgg ccttgtcgct ggccgtgacg acaagcggcg caattgtctg
     1681 cgcgtacgtt gcagaagaag aaacacacat ggcgagcacg atggctgcga ttgcatgggc
     1741 tgcggggcgc atggcattcc tccggttcag ggtgagtgaa cggccgagcc tactcgctcg
     1801 ggtgctatgc gacgtgaatt tcttcgcgct agagcgaggc agaaatgcgc tgcagagcgt
     1861 cgcgcagggt cgagtcgttc ttcgcgaagc agaagcggac gactttgcgg tcgtgcggtg
     1921 catcgtagaa caccgacacc gggatgaccg cgacgccgta cttgacgatc atcgttttgg
     1981 caaactcggg ctcgggtgcc gggctgagtg cggagtaatc agccagcacg aagtacgtgc
     2041 cttcgcaggg aaggacacgg aagggcgtgc ggcgcagccc gtcaatcagc aggttgcgct
     2101 tcgcctggta gaaggccggc aggtcgaggt acggcgatgg gtcctgcagg tacgcagcga
     2161 aggcgtgttg cgccggcgtg ttgacggcga acaccatgaa ctgatgcact ttgcggatct
     2221 cggcgctgat cgctgccggt gcgcaggcgt agccgacctt ccagccggtg gtgtggaacg
     2281 tcttgccgaa gctcgagatc acgaccgtgc gttcggccag cgcaggcgac cgcatcagcg
     2341 actggtgtgc cacgctgtcg aaaacgatgt gctcgtacac ctcgtccgac agcaccatca
     2401 cgccggttga ttcaacgatg ttctcgagag caacgagatc atccgaccgg agcacgcgcc
     2461 cggtcgggtt gtgcgggaaa ttgagcatca gcatccgcgt tctcggcgtg atggctgcgc
     2521 ggatgcgatc ccagtcgggg gcgtagccat tctcggcatc aaggggcacg tacaccggtc
     2581 gcccgcccgc cagctcgatc gaggggacgt agctgtcgta ggtcggttcc agcacgatga
     2641 cttcgtcgcc cggatgcacg agggcgagga cacttgccat gatggcctgc gtcgcgcctg
     2701 cggtgatggt gatctcggtg tctgcgtcgt agcgatgacc gtagagcgtg aggttcttcg
     2761 ctgcgatggc ggcgcgcaac gtgggtacgc cggccatcgg cgcgtactgg ttgtgcccag
     2821 cgcgcacggc atcctcaagg tgccgctgaa gaacggggtc acagttgaaa tccggaaagc
     2881 cctggcccag attgacggcg ccgtgcttga gcgcgagctg tgacatcacg gtgaagatcg
     2941 tggtgcccac ccgcggcaat cgggatttca gttgaagcga tgtaataaaa atccaggaaa
     3001 aacaggggct tagcgagcgg agaattatac tttgacgacg catcggccag caccatgaca
     3061 atcgacatca gcagcacctt tgacagcggc gcaatcgacg tggtcgcgac cgccgaagac
     3121 tcggccgaat tgcggattcg acacgacctg cggagcgatg gcagccgggc agagttcctg
     3181 cagtggtttc acttccgggt gagcggggcc gatggctccg gcgcgatcct gcgcgtgacc
     3241 aacgccggtg aaagcaccta tcccggaggt tggcaggact atcgcgccgt ggccagcgtc
     3301 gaccgccgac gctgggaacg ggtcccgacc cacttcgacg gcaaggtgct gaccatcgag
     3361 ttgcctccgg cgccggccac gtatgtcgcc tacttcgagc cgttttcgtg ggaccggcat
     3421 cttgcgctgc tggcagactg cgtggcacgc ggcgctcgtc agcgccggct ggggagcagc
     3481 ctgcagggtc gcgacctcga cgaactcatg atcggccagg gcgcgcggcc catctggatc
     3541 atcgcgcgcc agcatccggg cgagacgatg gccgagtggt tcgtcgaagg tctgatgcag
     3601 cggctgctcg acccggccga tgctgtcgcc cgcgagcttc gccagatggc cacctttcac
     3661 atcgttccga acatgaatcc cgacggctcc gtcctcggca acctgcgcac caacgccgcc
     3721 ggcgcgaacc tcaaccgcga atggctggag ccgacgcccg aacgcagccc cgaagtccag
     3781 gtggtgcgcg atgcgattca tgcgagcggg tgtgcgtcgt tcttcgacat ccacggcgac
     3841 gagaacctgc cgtacgtctt cgtggcgggc tgcgagatgc tgcccggatt cagcgaccgg
     3901 caggcggccg agcaggccgc attcagcgcg gacttccgga cggcgagccc ggacttccag
     3961 agcgagcacg gctacgccgc gggcaagtac aaggaagacg tgctgaagct cgcgtccaag
     4021 tatgtcggcc ataccttcgg gtgcctgtcg ctgacgctcg aaatgccgtt caaggacaac
     4081 gccaacgcgc ccgacgaaca gaccggatgg agcgccgccc gcagcgcccg cctgggtgcc
     4141 gacatgctgg gccccattcg acagcacctg atgcgcactt cggcatgacg acgatcgacg
     4201 ggcccaaggt gacgctcaag cgcgcgcacg cgaacgaccg cgcacgcgtg tacgagtggc
     4261 tgatcggttc cgaccttacg cagaacatga tgggtggcgc gatgtttccg gaccacccga
     4321 tcccttcgct cgacgcgttc acggccgact acgccgagca ctacttcgac ggcacgaggc
     4381 cctatgccgg ccggacgctg atcatcagcg cgaacggcga agacatcggt tgcgtctcgc
     4441 acgggccgat cgacgtactg aacgatgtcg tcgaacttga tatctggctg gcggagcgcc
     4501 gcgccgccgg gcagggctac ggttcggagg cgctagtcgt gctatgcgac tggctgcagt
     4561 cgaactatgg ggtcaaccgc tttctggtac ggccctcgcg ccgcaacgtc aaggcgctgc
     4621 gcgcgatgcg ccgcgccggc ttccgcgaga ccgacctgcc ggcgcgggac gtcgaatcca
     4681 aactttcgct gcggccgggc cattatgcag acgagctgct gctgttccgc atcctgccgg
     4741 ttccgtcgag cgtgttccgt cccgatccga cgcagaccta cgtcttcatc gacagcgagt
     4801 tcaccagcct ggagtcgccg caactcatca gtgtcggcgc ggtggcgacc gactcgaccg
     4861 cgttctatgc ggaactcgcc ggctgggacc cgcgcacggc gagcgacttt gtcaacgaca
     4921 ccgtcatccc gctgctggac ggtgatgcag tgccgctgtc cgtcggcgcc gaggcactgg
     4981 gcgagtggct cggagagcgt gcacgacggg gagcgacgac gatcatcagt gactcgggct
     5041 tcgatcgctg ggctctggcc gaattgttcg ggggcgaaaa cctgcccgcg aacgtacgct
     5101 ggcaacgcgt gcctgtggcc tacgaggcgc tcgacgaagc gacgcagcga ctgaacctgc
     5161 gtcggcacca cgcgctggat gacgcccgcg cactgcgcca gctagtgctc gaggcacagg
     5221 tgcccgcgcg gccggcctaa c
//
LOCUS       DATIRT010000459         7290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_668022, whole genome shotgun sequence.
ACCESSION   DATIRT010000459 DATIRT010000000
VERSION     DATIRT010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7290
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668022"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2532
                     /locus_tag="VLN25_10380"
     CDS             <1..2532
                     /locus_tag="VLN25_10380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSQ11001.1"
                     /translation="MRVLEVEGQPLRDDQGRYFQYALISPDITERKRTEAALRESAEY
                     FRALFDESPVAASIQAPDFRVVRVNSAHTRMLGYTIDQVIGKDPMTFSHPDDVDSVHA
                     SRRALDSAERGPVSFERRMLTGDGRTIWVRGHAVRFSDASGERYTLTMLENITESKHV
                     EKVLREAKEIAESASRAKSQFLANMSHEIRTPMNGVLGMTELLLGTPLSDKQRRFADA
                     VYRSGEALLEIINDILDFSKIEAGKLELESVDFNLRTVVEDVFELLAPRAHQKRLELA
                     SRIEPAVPTVVRGDPMRLRQILTNLVGNAIKFTESGEVIVSVGVAETPGDNGEHRVAF
                     EVRDSGIGMRPDALEKLFTVFMQADQSMSRRYGGTGLGLAISKQLVELMGGRISAVSR
                     FGEGSTFRFEVPLAAGEAAAVPPTVDAGRLRGTRVILVEDNPTNRSILESQLRDFGMD
                     VATADNGATALELMRAAARAGSPFDAAVVDMKMPIMDGLTMATELRRDPQLAEIRMVM
                     LTSLGSGNEARLAYDSGIEAYLTKPVRQAELIDALGRVLQHEEPAVPAVLTVSPGQRA
                     RVLIVEDNAVNQEVARAMLTELGCAIRLASDGREALTALREEEFDLVFMDCQMPEMDG
                     FEAVRRFRGSASTGYATRADVPIVALTANALAGDAERCLEAGFSDYLAKPVRREQLDT
                     ALTRWIREPVAPAVGTPDSVAPLPAAESVATAAPEAEARLTIDLTVIEVIRDMERRGA
                     SRLLERLVATYATTAARLVAQAAYALKSGDSASLQHAAHTLKSSSANLGAIELSRRFG
                     ALELHARAGALEAARTEWGATQLEYERVVQALQDIVAADEAVVTH"
     gene            2549..5044
                     /locus_tag="VLN25_10385"
     CDS             2549..5044
                     /locus_tag="VLN25_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSQ11002.1"
                     /translation="MNNEAPRTGKCAEEPRENGAAQRADLVLLVDDDPVARLLTASAL
                     AERQWRVIEADGGASALELYANNQPDVVVLDALMPDIDGFITCERLRRLSGGEHVPIL
                     MLTGLDDETSIARAYEAGATDFFVKSTTQWTLLSERLRYMIRASRMREELADSRAKLS
                     KAQRIARLGSWEWDIGARWVKLSEECFAIAGLPQQEDGLADWFVWSRVVEEERARIEM
                     LFREALAGSGQMNFECRLARPTGQVKVVQIEGEVDRDEAGRAKAVHGVIQDITERKQA
                     EDQIRQLANYDSLTGLPNRRLFRDQFQAALERARSSGSAVGVLFIDLDRFKQINDTLG
                     HQVGDQLLREVAKRLYQCVRENDTVARGAEATGALRNGRPPSIGSATRSLAAGSQTAP
                     VAGASNSVARLGGDEFTILLTDLAEPSAIEAVALRLLETMRRPFSFSGHELFVTASMG
                     VATYPIDGGDVDTLLRKADIAMYAVKDSGRNGVMRFSSAMNTATAERWRLETALHRAL
                     EREELVMYYQPKVNVIDGSIVGAEALMRWKRGGELVPPAEFITVAEESGLIVPITEWA
                     VREVCKQMVAWAEDGMPPMPVSINISGRHIQRANLVEPVQAALAGFRLDARLLELELT
                     ETVLMQNLGHALPLLQALKELGVAISVDDFGTGYSSLSYLKKLPIDTLKIDRSFVREL
                     ETSGDNAAIVAAIIAMSKSLKLRVVAEGVETQGQMTRLFDQGCQLMQGFLFSPAVPGG
                     DFHGLIRNVAGRQHWRVNFGPRRAATTPPEAMDQHSNGRHHGTIESEFVGPPRPPAEP
                     PRETLRAVVDNAEHGGQRDRALKWATRFIGRDG"
     gene            complement(5118..5759)
                     /locus_tag="VLN25_10390"
     CDS             complement(5118..5759)
                     /locus_tag="VLN25_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSQ11003.1"
                     /translation="MPVRLIVVDDHAIVRRGIVQVLSEHPDTQIVAEASDYGQLRAEL
                     KKHGEPDVLVMDVGLPGKNGIEILKSLREEYPRLKVLMVSMYPEDQYAVRAFRAGAFG
                     YLNKGSAPDKLLEALSMVIAGKKYVTPEIAQALIENLNAPEDAGAPHEKLSDREFQTL
                     KLMASGKRLSDIAEELALSPKTVSVYRARILEKMGLSNNAELTHYAIKHGLVE"
     gene            complement(5749..6879)
                     /locus_tag="VLN25_10395"
     CDS             complement(5749..6879)
                     /locus_tag="VLN25_10395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSQ11004.1"
                     /translation="MSTSDPAAPRLIVVEDSELDYELLLAMLMREGLRPRAVRVEDES
                     GMRDAFAQGTIDAVITDHNLPRFDSFASLKVAKAVDPDIPVIVLSGEMSEELAVASLH
                     AGADDFVLKTRMFRVAAALQHSLRAARTRREARGAAVALVESERKLHALTQHLERAKE
                     DERRAIAQEIHDDVGGALTALKFEVVRLARELGADPQHRDRMEAIQQLLESAVVATHR
                     IQHALRPAVLDAGLVAALEWLARGFGERTGLPIDFRSNRDEFDIAPDRAAALYRVAQE
                     ALTNVRKHAGARAVHVQLFALEDEVTLEIADDGAGFEPDSLAVTPGFGVRGLLERARG
                     LDGWAEVSSSPGRGTTVMFSIPPLPAARQRVAASIEEGPDAG"
BASE COUNT         1294 a         2332 c         2473 g         1191 t
ORIGIN      
        1 atgcgcgtgc ttgaagtgga agggcagccg ctgcgtgacg accaggggcg ctatttccag
       61 tatgcgttga tcagcccgga catcaccgag cgcaagcgca ccgaggcggc gctgcgcgaa
      121 agcgccgagt atttccgcgc attgttcgat gaatcgccgg tggcggccag catccaggcg
      181 ccggatttcc gtgtggtgcg agtgaactcc gcgcacacgc gcatgctcgg ttacacgatc
      241 gaccaggtga tcggcaagga tccgatgacg ttttcccatc cggacgacgt cgactcggtg
      301 catgcatcgc gccgcgcgct ggattccgca gagcgcggtc cggtgtcttt cgaacgccgc
      361 atgctgaccg gagacggccg gaccatctgg gtgcgcggac acgcggtgcg cttcagcgac
      421 gccagcggcg agcgctatac gctgaccatg ctcgagaaca tcaccgagag caagcacgtc
      481 gaaaaggtac tgcgcgaagc gaaggagatt gccgagtcgg ccagccgcgc gaagtcacag
      541 ttcctcgcaa acatgagcca cgaaatccgc acgccgatga acggcgtgct gggaatgacg
      601 gaactgctcc tcggaacgcc cctgtcggac aagcaacgtc gcttcgccga tgcggtctat
      661 cgctcaggcg aagcgctgct ggagatcatc aacgacatcc tggatttttc caaaatcgag
      721 gccggcaagc tcgaactcga atcggtcgac ttcaacctgc gcacggtggt tgaggacgtg
      781 ttcgaacttc tcgcgccgcg cgcacatcag aagcggctcg agctggcgtc acgcatcgaa
      841 ccagccgtgc cgactgtggt gcgtggcgac ccgatgcgac tgcgccagat cctgacgaat
      901 ctggtcggca atgcgatcaa gttcaccgaa agcggcgagg tgatcgtctc ggtcggcgtc
      961 gccgaaacgc cgggggacaa cggcgagcat cgcgtcgcct tcgaggtgcg cgacagcggc
     1021 atcggcatgc ggcccgatgc gctggaaaag cttttcaccg tgtttatgca ggctgaccag
     1081 tcgatgtcgc gtcgctatgg cggcaccggg ctcggactgg cgatcagcaa gcaactggtc
     1141 gagctcatgg gcggccgcat ttctgccgtc agccgctttg gggaaggatc caccttccgc
     1201 ttcgaggttc cgctggctgc cggcgaggcc gccgcggtcc cgccaacggt cgacgccggc
     1261 cgcctgcgcg ggactcgggt gatcctggtc gaggacaacc cgacgaatcg cagcattctc
     1321 gagtcccagc ttcgcgactt cggcatggac gttgccaccg cggacaacgg cgcaaccgcg
     1381 ctcgaactga tgcgggcggc ggcgcgggcc ggctctccgt ttgatgcggc cgtggtcgac
     1441 atgaagatgc cgatcatgga tggcctgacc atggccactg aactgcggcg cgatccgcag
     1501 ctggccgaaa tcaggatggt gatgctgacc tcgctgggca gtggcaacga ggcccggctt
     1561 gcctacgaca gtggcatcga ggcctacctg accaagccgg tacggcaggc cgaactgatc
     1621 gatgcactgg gccgcgtcct gcaacacgag gagcctgccg tgcccgccgt cctgacggtg
     1681 tcgcccggcc aacgtgcgcg cgtgctgatt gtcgaggaca acgccgtcaa tcaggaggtc
     1741 gcccgcgcca tgctgaccga gctcggctgc gcgatccggc tggcgtccga cgggcgcgaa
     1801 gcgctgacgg cattgcgtga ggaggaattc gacctcgtct tcatggattg ccagatgccg
     1861 gagatggacg gcttcgaggc cgtccggcgc ttccgcggct cggcgtcgac cggatacgcg
     1921 acacgcgcgg acgtaccgat tgtcgccctg accgctaatg cgctggcggg agatgcggag
     1981 cgttgcctgg aagccggatt cagcgactac cttgcgaagc cggttcgacg cgagcagctc
     2041 gataccgcgt tgacgcgctg gatccgtgag ccggttgctc cggccgtcgg cacgcccgac
     2101 tccgttgctc cattgcccgc agcagagtct gttgcgacgg cagctcccga agccgaggcg
     2161 cgcctgacga tcgacctgac cgtcattgaa gtgatccgcg acatggagcg ccgtggtgcc
     2221 agccggctgc tcgaacggct cgtcgccacc tacgcaacga cggccgcacg gttggttgcc
     2281 caggccgcgt atgcactgaa gtccggtgac agtgcctcgc tccagcacgc ggcccacaca
     2341 ctgaagtcat ccagcgcgaa tctgggcgcg atcgaactct cgcgccgttt cggcgcgctc
     2401 gaactccatg cgcgggcagg cgctctcgag gccgcccgga cggagtgggg cgcaacgcaa
     2461 ctcgagtacg agcgcgtcgt gcaggctttg caggacatcg tcgccgctga cgaagcggtc
     2521 gtgactcact gaggatgaag tgcgagtaat gaacaacgaa gctccacgaa caggcaagtg
     2581 cgccgaggag ccacgcgaga acggcgcggc acagcgggcc gacctggtgc tgctggtcga
     2641 cgacgatcca gtggcacggc tgctgacggc ctccgcactg gccgagcggc agtggcgcgt
     2701 cattgaagcc gatggcggtg ccagtgcact ggagctgtac gcgaacaatc agccggacgt
     2761 cgtcgtgctg gatgcactca tgccggatat cgacggcttc atcacctgcg agcgcctgag
     2821 acggctgtca ggcggtgagc atgtcccgat cctgatgctg acgggtctcg atgacgagac
     2881 ttcgattgcg cgcgcctacg aggccggcgc gactgacttc ttcgtcaaga gcacgacaca
     2941 gtggacactg ctgtcggagc ggctgcgcta catgatccgt gcctcacgca tgcgcgagga
     3001 actggccgac agtcgcgcga agctgagcaa ggcgcagcgc atcgctcgcc tcggcagctg
     3061 ggagtgggac atcggtgccc gctgggtcaa gctgtcggaa gagtgcttcg ccatcgccgg
     3121 actaccgcag caggaagacg ggctggcgga ctggttcgta tggtcgcgcg tggtcgagga
     3181 agagcgcgcg cgcatcgaga tgctgttccg cgaggccctt gcgggcagcg gccagatgaa
     3241 cttcgagtgc cggctggcgc ggccgaccgg gcaggtaaag gtcgtgcaga tcgagggcga
     3301 agtcgaccgc gacgaagccg ggcgcgccaa ggccgtgcat ggcgtcatcc aggacatcac
     3361 ggagcgcaag caggcggagg atcagatccg ccagctggcg aactacgaca gtctcaccgg
     3421 cctgccgaat cgccggttat ttcgcgacca gttccaggcc gcgctcgaac gcgcacggtc
     3481 cagcggatcg gcggtcgggg tgctgttcat cgacctcgat cgcttcaaac agatcaacga
     3541 cacgctcggg caccaggtcg gcgaccagct gctgcgcgaa gtcgccaagc gcctgtacca
     3601 gtgtgtgcgc gagaatgaca cggtggcgcg cggggcggag gcgacgggcg ccttgcgcaa
     3661 tggacggcca cccagcatcg gatcggcaac acgtagcctc gcggcggggt cacagaccgc
     3721 accggtcgcc ggcgcgtcca acagcgtggc gcggctcggt ggcgacgagt tcacgatcct
     3781 gctgacggat ctcgccgaac ccagtgcgat cgaagcggtc gcactgcgcc tgctcgaaac
     3841 catgcgccgg ccattttcgt tttcaggaca cgaactgttt gtcactgcgt cgatgggtgt
     3901 ggcgacctat cccattgacg gcggcgatgt cgacacgctg ctgcgcaagg ccgacatcgc
     3961 gatgtatgcg gtcaaggaca gcggccgcaa cggcgtgatg cgcttttcga gcgcgatgaa
     4021 taccgccacc gcggaacgct ggcggctgga gacagcactg caccgcgcgc tcgagcgcga
     4081 ggaactggtg atgtattacc agcccaaggt caacgtgatc gatggcagca tcgtcggggc
     4141 cgaggccctg atgcgctgga agcgcggcgg cgaactggtc ccgcccgccg agttcatcac
     4201 cgtggccgag gaatccggcc tgatcgtgcc gatcaccgaa tgggccgtgc gcgaggtgtg
     4261 caagcagatg gtcgcgtggg ccgaagatgg catgccaccg atgccggtgt cgatcaacat
     4321 ctcgggtcgc cacatccagc gcgccaacct ggtcgaaccg gtccaggcag cgcttgccgg
     4381 tttccggctc gatgcgcgcc tgctcgaact ggaactcacc gagaccgtgc tgatgcagaa
     4441 cctcggccac gcgctgccgc tgctgcaggc gctcaaggaa ctcggcgtcg cgatctccgt
     4501 ggacgacttc ggcaccggct attcgtcgct gtcgtacctg aagaagctgc caatcgacac
     4561 gctcaagatc gaccgctcgt tcgtgcgcga gctcgagacc agcggagaca acgcggccat
     4621 cgtggccgcc atcatcgcga tgagcaagag cctgaagttg cgcgtggtgg ccgagggtgt
     4681 cgagacgcag ggtcagatga cgcgactgtt cgaccagggc tgccagctga tgcaggggtt
     4741 cctgttttcg ccagccgtgc cggggggcga cttccacgga ctgatccgca atgtggcagg
     4801 gcgtcagcat tggcgggtga acttcggtcc gcgccgtgcg gcgacaacgc cgcctgaagc
     4861 tatggaccag cattcgaacg gccgtcacca cggcacgatc gagtccgagt tcgttggacc
     4921 gccgcggcct cctgccgagc cgccgcgcga gacactgcgc gccgtcgtcg acaacgccga
     4981 gcacggtggg cagcgcgatc gcgcgctcaa gtgggcaacg cgcttcatcg gccgcgacgg
     5041 ctgatacctg gaacgggtcc gccttcgcgc cgcgggccgg ctgcgtgcgg gcgcggcttc
     5101 gggcgtatcg gccgggtcta ctcgaccagg ccgtgcttga tggcgtagtg ggtcagttct
     5161 gcgttgttcg acaggcccat tttttcgagg atgcgggcgc ggtagacact gacggtcttg
     5221 ggactgaggg cgagttcctc ggcgatgtcg gacaggcgct tgcccgacgc catcagcttc
     5281 agcgtctgga actcgcggtc ggacagtttt tcatggggcg caccggcatc ctccggcgcg
     5341 ttcagattct cgatcagcgc ctgcgcgatc tccggagtga cgtacttctt tccggcgatc
     5401 accatggaca gagcctcgag cagcttgtcg ggtgcgctgc ccttgttcag atagccgaac
     5461 gcgccagccc ggaaggcacg caccgcatac tggtcctccg gatacatgct gaccatcaac
     5521 accttcaggc gcgggtactc ctcgcgcagc gacttgagga tctcgatgcc atttttcccc
     5581 ggcagtccga cgtccatcac cagaacgtca ggctcgccgt gcttcttcag ttcggcacgc
     5641 aattgtccat agtccgacgc ctcggcgacg atctgcgtgt cggggtgttc ggacagcacc
     5701 tgcacgatgc cgcgccggac gattgcatgg tcgtcgacga cgatcagcct aaccggcatc
     5761 gggtccctcc tcgatcgaag ccgcaacgcg ctggcgtgcg gccggtagcg gcggaatcga
     5821 gaacatcacg gtggtgcccc ggccgggcga cgacgacact tcggcccagc catcgagtcc
     5881 gcgggcgcgc tcgagcaggc cgcgcacgcc gaagcccggc gtgacggcaa ggctgtccgg
     5941 ctcgaaacca gcaccgtcgt cggcgatctc gagcgtgact tcatcctcga gagcgaacag
     6001 ctgcacatgg accgcgcgcg ccccggcgtg cttgcgcaca ttcgtcagcg cttcctgcgc
     6061 gacgcggtag agcgctgctg cacggtccgg cgcgatgtcg aactcgtcgc gattgctgcg
     6121 gaaatcgatc ggcaggccgg tacgttcgcc gaagccgcgc gccagccact ccagcgcggc
     6181 gaccaggccg gcgtcgagca cggccggccg cagcgcgtgc tggatgcggt gcgtcgccac
     6241 caccgcggat tcgagcaact gctggatcgc ctccatgcgg tcgcgatgct ggggatcggc
     6301 gccgagttcg cgcgccagtc gcaccacttc gaacttcagc gccgtcaggg cgcctcccac
     6361 gtcgtcgtga atttcctgcg caatggcgcg gcgctcatct tccttcgcgc gctccagatg
     6421 ctgcgtcagc gcatgcagct tgcgttcgct ctccaccagc gcaactgcgg cgccacgtgc
     6481 ctcgcgccgc gtgcgcgcag cacgcaggct gtgctgcagg gccgccgcga cgcggaacat
     6541 ccgcgtcttc aggacgaagt catcggcacc cgcgtgcagg gaggctactg ccagttcctc
     6601 ggacatctcg cccgacagaa cgatgacggg gatgtcgggg tcgacggcct tggcgacttt
     6661 cagcgatgca aacgaatcga agcgcggaag gttgtgatcg gtgataacgg cgtcgatcgt
     6721 gccctgcgca aatgcgtcgc gcatgccgga ctcgtcttcg acgcgaacgg cgcgcggacg
     6781 cagaccctcg cgcatcagca tcgcgagcag cagctcgtag tcgagttcgg aatcctcgac
     6841 gacgatcagg cgcggggcag cagggtcgga cgtggacacg gtggccggga ttgtggcgga
     6901 ttgcgatggg gaatgcagga cgctacgcgc ccggggtgac ctccgctgcc atgaaaagcc
     6961 ccgtgcttcc ggtccaggtc gattgcatgc gccagccgtt gaatccgtcg gacgcaggct
     7021 aaaatgcgaa ctgcaaagta cgccgggcga tcgcggtgtc ggcacgccga catcgaggaa
     7081 agtccggact ccgcagagcg acgtagcagg taacgcctgc ccaccgcgag gtgaggatca
     7141 gagcaacaga gacgagtcga ccggctggag gcgcgcgtag cgcgctgaca aagtctcgtt
     7201 aaggagggcg gccgcaaggc ggttttcctg aagggagcgg cgcgcaagcg gcgcatccga
     7261 cgaggcaagg ccgagtcggg tgaaacgggc
//
LOCUS       DATIRT010000460         4751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_77524, whole genome shotgun sequence.
ACCESSION   DATIRT010000460 DATIRT010000000
VERSION     DATIRT010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77524"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="VLN25_10400"
     CDS             complement(<1..771)
                     /locus_tag="VLN25_10400"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HSQ11005.1"
                     /translation="MNFIGGSSRLCLAMLLTATALVAGCGKKDDPIAQAGKPDAAKGI
                     AAPGLAETRAIAEEGFSYGLPLVMNYAVMNEFVVDKNSGQYKAPFNTISNEARVFTYK
                     DTAVVTPNSDTPYSMLWLDLRAEPMVISVPAVAKGRYYSVQLSDGNAYNYGYIGSRAT
                     GIDAGNYLVVGPDWKGERPAGIRKVFQSTTPYALSIFRTQLFNAGDMPNVVKVQAGYK
                     ALPLSTFLKQPAPPPAPKVEFLPATTAGIKENFYHYLDA"
     gene            complement(891..1490)
                     /locus_tag="VLN25_10405"
     CDS             complement(891..1490)
                     /locus_tag="VLN25_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HSQ11006.1"
                     /translation="MHASTASVDTGDNGGDKNAISATQHWLEQAVIGLNLCPFARAVH
                     VKGQIRWVESGARDTQDLLDDLVRELQFLAAADPEKVDTTLLIHPLVLTDFLDYNDFL
                     DVADAAIEELGYGGVLQVASFHPDYRFEGTAADDMGNFTNRSPHPMLHLLREDSIARA
                     VAACPDAAAIYERNVETLHRLGAAGWHGLMSSAGRRLEE"
     gene            complement(1523..3334)
                     /locus_tag="VLN25_10410"
     CDS             complement(1523..3334)
                     /locus_tag="VLN25_10410"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HSQ11007.1"
                     /translation="MSDITILFLIIAVVIVLFITEKVPVVIVALGTALSLYFTGILDF
                     RQALGGLGDPTTIFIASLFVVSAGLEVTGVTAWAGQFLITKAGDSRVRQLVLIMTACA
                     LLTGLISLNGAVAALLPVVVVMAVRLNRPPSQLLMPMVFASHAGSILALTGSPVNVLV
                     SEAAADAGTGNFGFFEFAIVGAPLLIGTMAILAFLGNRLLPHKSGASMPTDLSKHART
                     LVEQYRLVGNAFQLRVRTSSPLIGTAPNAIDLAAYPGLSLIGSQTGATGRPLTRGAIE
                     EGDTLLLRGEADAAASFASDQHLAFGPGEAEAGVADTLFNRSSGLAEVVIPPRSAMVG
                     SEVFPGMVTESGDLLILAVQRRGEDVGPGKVALEVGDSMLLQGTWTALDKRLADPEVL
                     VVHSPDLVRRQAVPMGKGAKAAIAILIAMVLALALNLVPSAVAGLVAAVAMVLIGVLS
                     VDQAYRSVNWTTVILVGAMTPLSAAMAETGAAQLLADKLVGIVGEAGPYALLAGLFVL
                     TAVLGQLISNTATALIIIPIAVAAALELGISPRPVMMSVCVAAAASFLTPVATPVNLM
                     IMGPGGYKFGDYWKIGIVCMAWFFVMAVFFVPMVWGF"
     gene            complement(3453..>4751)
                     /locus_tag="VLN25_10415"
     CDS             complement(3453..>4751)
                     /locus_tag="VLN25_10415"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HSQ11008.1"
                     /translation="KDGKSVVIAIGVGDAGLDVARLRDAAAAFARAASRQSHLATGLA
                     DVASGVAPAAAAQAVTEGVLLARYRYEQLKSKKSGVPLEQLTLVTTAERAKAETAGAK
                     SGQTMALAAMLARDLANAPAKELPATRMAEIAESVARDRGLQVEVFGKADLEKMGCGG
                     ILGVNAGSTEPPRMVKLTYKPKGGKGKGGHLAMVGKGIMYDSGGINLKPAQGMQLFMK
                     MDMAGAAAVLGAMSVLRDLDCPNTVTAFLMCTDNMPSGSATKMGDVLTQRNGKTVEIH
                     NTDAEGRLILADGLALAAEMKPDAIINIGTLTGAILAALGELLTGVFSNSDAFAAQVQ
                     ASASRTDETIWRMPLDRRYRKQIDSAIADLKNMGDAQPGSITAALYLEEFVDGIPWTH
                     FDICGTMKSDTDDSWRSKGATGVGARLLADLAVNFAPPRR"
BASE COUNT          832 a         1523 c         1535 g          861 t
ORIGIN      
        1 ggcgtcgaga tagtggtaga agttctcctt gatcccggcc gtcgtcgcgg gcagaaactc
       61 gaccttgggc gcgggaggcg gcgcgggctg cttcaggaac gtcgacagcg gcagtgcctt
      121 gtatcccgcc tgcaccttca cgacgttcgg catgtcaccg gcgttgaaga gctgggtccg
      181 gaaaatggag agcgcgtacg gcgtcgtcga ctggaacact ttcctgatcc ccgccggcct
      241 ctcacccttc cagtcgggac cgacgacgag gtagttaccg gcgtcgatgc ccgtcgcacg
      301 gctgccgatg tagccatagt tgtaggcgtt gccatcggac agctgcaccg agtagtagcg
      361 gccctttgcc acggcgggta ccgagatcac catcggctcc gcgcgcaagt ccagccagag
      421 catcgagtaa ggcgtgtcgc tgttgggcgt caccacggcc gtgtccttgt aagtgaacac
      481 gcgggcctcg ttgctgatcg tgttgaacgg cgccttgtac tggcccgagt tcttgtccac
      541 gacgaactcg ttcatcaccg cgtagttcat caccagcggc agcccgtaac tgaacccttc
      601 ctccgcgatg gcccgggtct cggcaaggcc cggtgcggcg atgcccttgg ccgcgtccgg
      661 cttcccggcc tgggcgatgg ggtcgtcctt cttgccgcag ccggccacga gtgccgtcgc
      721 cgtcaggagc atcgcaaggc acagtctcga agatccacca atgaaattca tctttgtaaa
      781 tgctcctgac cctacgggga agttgatgcg cggtcgtcgg aagtttgatt ggcgagccta
      841 acggaattgt cgatctgtga cggtccactg cccgatttgc aaggatgcgc ctactcctcg
      901 agccgacgac ccgcgctcga catcaacccg tgccagccag cggcgcccag acgatgcagg
      961 gtctcaacat ttcgttcata gatggcggcg gcatccggac atgcggcgac ggcgcgggcg
     1021 atgctgtcct cgcgcaacaa gtggagcatg gggtggggcg agcgattggt gaagttgccc
     1081 atatcgtcgg cggccgtgcc ctcgaaccgg tagtcggggt ggaagctggc gacctgaaga
     1141 acgccaccgt aacccagttc ctcgatggcg gcgtcggcca catcgaggaa gtcgttgtag
     1201 tccaggaagt cagtcaacac gagcgggtgg atcagcagcg ttgtgtccac cttctcgggg
     1261 tcggccgccg ccaggaactg cagttcacgc accaggtcgt cgagcaggtc ctgcgtgtcg
     1321 cgcgcaccgc tttcgaccca gcgaatctgt cctttgacgt gcaccgccct ggcaaacggg
     1381 cagagattca gcccgatgac ggcttgttcg agccagtgct gtgttgcgga gatggcgttc
     1441 ttgtcaccgc cgttgtcgcc ggtgtccacc gacgcggtgg acgcatgcat gggctgcctc
     1501 atccagtccg atgccgcatt cctcagaaac cccagaccat cggcacgaag aacacggcca
     1561 tcacgaagaa ccaggccatg cagacgatac cgatcttcca gtagtccccg aacttgtagc
     1621 cacccggccc catgatcatc aggttgacgg gcgtggcgac gggtgtgagg aacgatgccg
     1681 cggcggccac gcagacgctc atcatgaccg gacgcggcga gataccgagc tcgagcgcgg
     1741 ccgcgaccgc aatcggaatg atgatcaacg ccgtggcggt attgctgatg agctggccga
     1801 gcacggccgt cagcacgaag agtcccgcca gcagcgcgta gggacccgcc tcgccgacga
     1861 tcccgaccag cttgtctgcg agcagttgcg ccgcaccggt ttccgccatg gcggccgaca
     1921 gcggggtcat tgcgccgacc aggatcaccg tggtccagtt gaccgagcga taggcctgat
     1981 cgaccgagag cacgccgatc agcaccatcg ccaccgcggc aaccagcccg gcaacggcgg
     2041 aaggcacgag gttgagcgcc agtgcgagga ccatcgcgat caggatggcg atggctgcct
     2101 tcgcgccttt ccccattggc actgcctgtc tgcgcacgag gtcaggagag tggaccacca
     2161 gcacttccgg atcggcgagt cttttgtcaa gggccgtcca cgtgccctgc aggagcatcg
     2221 agtccccgac ttcgagtgcg accttgcccg gaccgacgtc ttcgccgcgc ctctgcaccg
     2281 caaggatcag caggtcgccg ctctcggtca ccatgcccgg gaagacctcg cttccgacca
     2341 tcgcggaacg tggcggaatg accacttccg cgagtcccga cgagcgattg aatagcgtgt
     2401 cggccacgcc agcctcggcc tcgcccggac caaaggccag atgctgatca ctcgcgaagc
     2461 tcgctgcggc gtcggcttcg ccgcgcaaga gcagcgtgtc gccttcttca atcgccccgc
     2521 gtgtcagcgg ccgccccgtg gcgccggtct gcgaaccgat caggctcagt ccggggtagg
     2581 cggcgagatc gatggcgttt ggcgccgtgc cgatcagggg tgaactcgtt cgcacacgta
     2641 actggaacgc gttgcccact agacggtact gttcgaccag cgtgcgggcg tgcttggaca
     2701 ggtcagtcgg catcgacgcg ccgcttttgt gcggcagcag gcgatttccg aggaacgcca
     2761 ggattgccat cgtgccgatg agcaagggcg cgccgacgat cgcgaactcg aagaaaccga
     2821 agttccccgt gccggcgtcg gcggctgcct cggacaccag cacgttcacc ggtgaaccgg
     2881 tcagcgcgag gatcgaaccg gcatgcgatg cgaacaccat cggcatcagc agttgtgacg
     2941 gcggtcggtt cagccgcacc gccatcacca ccacgaccgg cagcagtgcc gcgaccgcgc
     3001 cgttcaggct gatcaggccg gtcaggagcg cgcaggccgt catgatcaat accagttgcc
     3061 tgacccggct gtcgcctgcc ttggtgatga ggaactgtcc cgcccaggcg gtcactcccg
     3121 tcacctcgag tccggcactg acgacgaaga gcgacgcgat gaagatggtc gtcgggtcgc
     3181 caagtccgcc gagtgcctgg cgaaagtcga ggatgccggt gaagtacagc gaaagtgccg
     3241 tgccgagagc gacgatcact accggaactt tttcagtgat gaacagcacg atcaccactg
     3301 cgatgatcag aaagagaatg gtgatatcgc tcaagggttc acctgttgtt gtagtggcct
     3361 ttgatggctg gactgtaact ccgccgcggc aggctgcgac atagtgggtg cacgcgtggc
     3421 cgtgaacctc gcgtgctgcg catgcaccgt catcagcgtc gcggcggcgc gaagttcacc
     3481 gcgaggtcgg cgagcaggcg tgcgccgacg ccggtggctc ccttcgagcg ccacgaatcg
     3541 tcggtgtcgg acttcatcgt tccgcagatg tcgaagtgcg tccatggaat gccgtcgacg
     3601 aattcttcga gatacagcgc cgcggtgatc gaacccggct gcgcatcgcc catgtttttt
     3661 aggtcggcga tagccgaatc gatctgcttg cggtagcgcc ggtcgagcgg catgcgccag
     3721 atcgtttcgt cagtgcggct ggccgatgcc tggacctgcg cagcaaaggc gtcgctgttg
     3781 gagaacacgc cggtcagcaa ttcgccgagc gcggcgagga tcgcaccggt cagcgtgccg
     3841 atgttgatga tcgcgtcggg tttcatttcc gctgcgagcg caaggccgtc agcaaggatc
     3901 aggcggcctt cggcgtcggt gttgtggatc tcgacggtct tgccgttgcg ctgggtcagc
     3961 acgtcgccca tcttcgtcgc cgagcccgac ggcatgttgt ccgtgcacat caggaaagcg
     4021 gtaacggtgt tcgggcagtc gagatcacgc agcacggaca tcgcaccgag cacggcggcg
     4081 gcaccggcca tgtccatctt catgaagagc tgcatgccct gcgccggctt aaggttgatg
     4141 ccgccagagt cgtacatgat gcctttgccg accatcgcca ggtgtccgcc cttgcccttg
     4201 ccacccttcg gcttgtaggt gagcttgacc atgcgtggcg gctcggtgct gccggcattg
     4261 acgccgagga tgccgccgca gcccatcttt tcgaggtcgg ccttgccgaa cacttcgacc
     4321 tgcagcccgc ggtcgcgcgc cacgctctcg gcgatctcgg ccatgcgcgt ggcgggcagt
     4381 tctttcgcag gggcgttggc caggtcgcgc gcgagcattg cggccaaggc catcgtctgg
     4441 ccggactttg cgcccgctgt ttccgctttc gcgcgctcgg cggtcgtcac cagtgtgagt
     4501 tgctcgaggg gtacgccaga tttcttgctc ttcaattgct cgtagcggta gcgcgccagc
     4561 agcacgccct cggtgacggc ctgtgcggcg gccgccggcg cgacgccact ggcaacatcg
     4621 gcaaggccag tggccagatg cgactgcctg gacgcggccc gcgcaaaggc agcggccgca
     4681 tcacgcaatc tggcgacatc gaggccggca tcgccgacgc ctattgcaat gacgaccgac
     4741 tttccatcct t
//
LOCUS       DATIRT010000461         7451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_472750, whole genome shotgun sequence.
ACCESSION   DATIRT010000461 DATIRT010000000
VERSION     DATIRT010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7451
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472750"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..461)
                     /locus_tag="VLN25_10420"
     CDS             complement(213..461)
                     /locus_tag="VLN25_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11009.1"
                     /translation="MNVFEPDGLWEAVRERLARPGALGWLTLIPVVGAFAVAIAAETG
                     VRVFNTDWHAVFGYSARPDTDGSRFRALWVALALAPYI"
     gene            complement(478..981)
                     /locus_tag="VLN25_10425"
     CDS             complement(478..981)
                     /locus_tag="VLN25_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HSQ11010.1"
                     /translation="MELGRTLRHLLTTPGAVRRAFPEEALVRIRAAIAASEAAHSGEI
                     RFAVEAALPWSYLRRDAPARERAQMIFSKLRVWDTASNNGVLIYVELADHQIEIVADR
                     GIGAHVTAQEWTRIADAMRERFRSRAFEAGVIEGVQAVVALLARHFPLADGQINPNEL
                     PDSPAVL"
     gene            complement(987..1943)
                     /locus_tag="VLN25_10430"
     CDS             complement(987..1943)
                     /locus_tag="VLN25_10430"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HSQ11011.1"
                     /translation="MAAPLAALAFALLAAVAAAQQAPVPLPKGVFPGAEPSAAPPTAP
                     AQSAPTPAPTPQVEPAATGGPAGRDLARDSLGRVAVPPAAPVIDLAGVLPPAALHTLR
                     SKLIEFERASGSQIAVIVVPTTQPEPIADFTNRVGDAWKIGRKGVGDGVLIAVAVQDR
                     KVWISVARALEGAIPDVVASRITREFMGPSFAKGDFAGGINAGVDAVMKRIEGEALPT
                     PAGIPRHKVDAGEDLMAALVPLVIFGAIVAGLLRRAFGTPGAAVTSMGVGALAGVLLT
                     SLVMGAIAAVAVFVFSAFFGGGGGWSSGGGGDFAGGGGGSSW"
     gene            complement(1993..2592)
                     /locus_tag="VLN25_10435"
     CDS             complement(1993..2592)
                     /locus_tag="VLN25_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HSQ11012.1"
                     /translation="MKALRGLFAVILASLLAGCGYNTIQQRDEAVKSAWAEVLSQYQR
                     RADLIPNIVSTVKGEADFEKTTLQNVIEARAKATAIQATPELVNNPEAFAKFTQAQGE
                     LSSALSRLLVTVENYPNLKANAGFQELRTQLEGTENRIGIARNRYIKSVEDFNSFIRQ
                     LPQNLTAMVFGYKPKPQFAVENESAISKPPTVDFGAPKK"
     gene            2742..3263
                     /locus_tag="VLN25_10440"
     CDS             2742..3263
                     /locus_tag="VLN25_10440"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HSQ11013.1"
                     /translation="MQAKAHDTDYRRALAQFATGVTVVTTRDSQGAPVGLTVNSFNSV
                     SLEPPLVLWSLALKAGSLAAFEACSHYAVNVLAGQQLGVAQRFATRGANRFAPADWHD
                     GPHGMPLLHGCVATLIVRNRSRHPEGDHVILVGEVVQFDAPGGTPLVFHDGRYFASAV
                     EEKLPRALRTPWR"
     gene            3375..4271
                     /locus_tag="VLN25_10445"
     CDS             3375..4271
                     /locus_tag="VLN25_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HSQ11014.1"
                     /translation="MKRRDLSDLALLAALWGASFLFTRMAVPAFGAFALAELRVAIAA
                     LMLLPLLALRGDFSELRAAPARFVLLGAVNTAIPFALFAWAVLSITAGLASILNATAP
                     LFTALVAWVWLRERLTLLQWVGMGIGFAGVLWLAGAKAGFGGDSSGWAIIAALVATFS
                     YGVSASVAKRKFVGVRPLAVAAGSQTAAALLLLPFAIATWPSEPPAVIDWAAATALGV
                     LCTGIAYILYFRLIARVGPATAMTVTYLIPAFAMLWGGIFLAETVTPTMLIGCAIILA
                     GTALATGTLRPRRSASTSAGSS"
     gene            4335..4967
                     /gene="kynB"
                     /locus_tag="VLN25_10450"
     CDS             4335..4967
                     /gene="kynB"
                     /locus_tag="VLN25_10450"
                     /EC_number="3.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_038735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylformamidase"
                     /protein_id="HSQ11015.1"
                     /translation="MLRLWDISPTIGPGVPVFPGDTPFAQRWTWTLDVRCPVNVSEIT
                     LSPHTGAHTDAPLHYAADGAAMAEVPIQIYLGPCRVVHAIDVGSLIEPKHLLGDLDDA
                     PERVLIRTYLQAPLQVWDPAFSALAPSTIDALHARGVRLVGIDTPSLDPADSKSLDSH
                     QRVRAHGMAILEGIVLDDVPAGDYELIALPLKIAGCDASPVRAVLRELPA"
     gene            4964..6229
                     /gene="kynU"
                     /locus_tag="VLN25_10455"
     CDS             4964..6229
                     /gene="kynU"
                     /locus_tag="VLN25_10455"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HSQ11016.1"
                     /translation="MTRDEALARDAADPLAAHRRGFCLPEGVIYLDGNSLGALPLSTS
                     ARVREVIEREWGGGLIRSWNTARWTDLPRRVGAKIARLIGAKAHEVICADSTSINLFK
                     VLAAALHLQTARPQVSIADRRVILSERGNFPTDLYVAQGLTQLLGDRHELRLVDTDEV
                     HGAIDETVAVVLLTHVNFKSGAMHDLAALTRRAHEAGALVVWDLAHSAGAVPVDLDAA
                     QADFAVGCGYKYLNGGPGAPAFVYAAARHLEALAGATYAQPLTGWFGHRTPFEFVTHY
                     TPAETIDRFAVGTPPIIALAALDAGVDTVLAAGIDALRAKSVALTDAFIALVERDCKG
                     VTLATPRAAGQRGSQVCFAHADAFAVMQALIERGVIGDFRAPDILRFGFAPLYVRHVD
                     VFDAAQALADVLRSGAWRVERFQHRAMVT"
     gene            6226..7032
                     /gene="kynA"
                     /locus_tag="VLN25_10460"
     CDS             6226..7032
                     /gene="kynA"
                     /locus_tag="VLN25_10460"
                     /EC_number="1.13.11.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009952454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase"
                     /protein_id="HSQ11017.1"
                     /translation="MSRPADPPAMRYGDYLALDAILDAQHPRSEDPNELLFIVQHQTS
                     ELWMKLALHELRTAQAAIAASDLPPAFKMLARVSRIMEQLVHAWNVLATMTPSEYSAI
                     RPFLGTSSGFQSWQYREIEFLLGNKNAAMLRPHDDRPEMKQRLEAAFAAPSLYDDAIR
                     LLARRGFAIDPAVIERDLTQRHAGDPSVEAAWLAIYRAPREHWELYELAEELVDLEDA
                     FRLWRFRHVSTVERIIGFKPGTGGTSGVGYLRKMLEVVLFPELWKVRTDL"
BASE COUNT         1197 a         2590 c         2412 g         1252 t
ORIGIN      
        1 gaatgcggca catcgagcag ttcgcgtgca cccatcaccc accagacgaa gctgacggac
       61 aacgccccca gcaccaggat gattcccggc agccagacca tcgtcaggcc ggcgagatac
      121 aaaggcacag tgccgacaac ggcgaccgcg atggccgctc gccatcggcg ggggcaccca
      181 tacatgggaa gcaggaacgc accgacgacg ccctatatgt acggcgccag ggcaagggcg
      241 acccaaagcg cgcgaaagcg ggatccgtcc gtgtccggtc gcgccgaata gccgaacacg
      301 gcatgccagt ccgtgttgaa cacacgcact ccggtttccg cggcgatcgc aacggcaaac
      361 gcaccgacga cggggatcag ggtcagccag ccgagggcgc cgggccgtgc aaggcgttca
      421 cgcaccgcct cccacaagcc gtccggctcg aagacattca actagagcct gtacacgcta
      481 gaggacggcg ggagagtcgg gcagttcatt cggattgatc tggccatcgg ccagcgggaa
      541 atgccgggcc aataacgcga cgaccgcctg cacgccctcg atgacacccg cttcgaatgc
      601 tctcgagcga aaccgctcgc gcatggcatc ggcaattcgg gtccattcct gcgcggtcac
      661 atgggcgccg atgccgcggt ccgcgacgat ctcgatctgg tgatcggcca gttcgacata
      721 gatcagcacg ccgttgttgc tggcggtgtc ccagacacgc aacttcgaga agatcatctg
      781 cgcacgttcg cgcgccggcg catcgcgccg caggtagctc cacggcagcg cggcctcgac
      841 cgcgaatcgg atctcgccgc tgtgcgcggc ctcgctcgcc gcgatcgctg cgcggatgcg
      901 caccagcgcc tcttccggaa acgcgcgacg caccgcgccc ggagtggtca aaaggtgccg
      961 cagggttcgt ccgagttcca tggtgcttac cagctgctgc cgccgccccc accggcgaag
     1021 tcgcctccgc cgccggagct ccagcctccg cccccgccga agaaggcgga aaacacgaac
     1081 accgccacgg ccgcgatagc acccataacc agcgacgtca gcaggactcc cgccagggcc
     1141 ccgacaccca tgctggtgac tgcggcaccc ggcgtgccaa acgcgcgccg cagcaacccc
     1201 gccacgatgg caccaaagat gaccagcggc acaagtgcgg ccatcaaatc ctcgcccgcg
     1261 tcgaccttgt ggcgcggtat accggccggt gtcggcagcg cctcgccttc gatgcgcttc
     1321 atcacggcat cgactccggc attgatgcca ccagcgaagt cgcccttcgc aaaggaggga
     1381 cccatgaatt cgcgcgtgat gcgcgacgcc acgacgtccg ggattgcccc ttcgagcgca
     1441 cgcgccacgc tgatccagac cttgcgatcc tggaccgcca cggcgatcag cacgccatcg
     1501 ccgacgccct tgcgcccgat cttccacgca tcgccgacgc ggttggtgaa gtcggcaatc
     1561 ggttcgggtt gtgtcgtcgg cacgacaatg accgcgatct gagaaccgct cgcccgctcg
     1621 aactcgatca gcttgctgcg cagcgtgtgc agcgccgcgg gagggagtac gcccgccagg
     1681 tcgatcacgg gcgccgcagg aggaacagcc acccgaccga gggaatcacg cgcgaggtcg
     1741 cgtcccgccg gcccgcccgt cgctgccggc tcgacttgcg gcgtcggcgc cggggtgggt
     1801 gcggactggg cgggtgcagt cggcggagca gcgctcggct cggccccggg gaacacccct
     1861 ttcgggagcg ggacaggcgc ctgctgcgcg gccgcgaccg cggcgagcag cgcgaacgcc
     1921 agcgccgcga gcggcgcggc cactcgcctc atcgacccca tggagcctgc ccggtgcaga
     1981 gccgtttcgc cgttacttct tcggcgcgcc gaagtcgaca gtcggcggct tgctgatggc
     2041 cgattcgttt tccaccgcga actgcggctt cggcttgtag ccgaacacca tcgccgtcag
     2101 gttttgcggc agctgccgga tgaaactgtt gaaatcctcg accgacttga tgtaacgatt
     2161 gcgcgcgatg ccgatgcggt tttccgtacc ctcgagctgc gtgcgcagtt cctggaatcc
     2221 ggcgttggcc ttgaggttgg gatagttctc caccgtcacg agcaagcggg acagcgcgga
     2281 actcaattca ccctgcgcct gcgtgaactt cgcaaacgct tcaggattgt tgacgagctc
     2341 gggcgtggcc tggatggcgg tcgccttggc gcgcgcctcg atcacgttct gcagcgtggt
     2401 cttctcaaag tcggcctcgc ccttgaccgt gctgacgatg ttgggaatca ggtcggcgcg
     2461 ccgctggtac tggctgagca cttcggccca cgccgatttc accgcctcgt ccctctgttg
     2521 gatcgtgttg tagccgcagc cggccaaaag gctggccagt atcacggcaa agagtccccg
     2581 caatgccttc atcatgtcct ccgggttgta cgtcgtactg tgactttgtc gaacccgctg
     2641 aagacttctg cacagctggt ccgaactctg cagcggcgat catagcgttc ggcgcggcgc
     2701 cattcgcccg gcccgagtcg cccggcacgt agaatcgacg catgcaggca aaagcccatg
     2761 acacggatta ccgcagggcg cttgcccagt tcgccaccgg ggtcacggtc gtcaccacgc
     2821 gtgactccca gggcgctccg gtcggcctta ccgtcaattc ctttaattcg gtgtcactcg
     2881 agccgccgct ggtgctgtgg agcctcgcgc tcaaggccgg ttcgctggcc gccttcgagg
     2941 cgtgctccca ctacgccgtg aacgtgctcg ccggccagca gctcggcgtg gcccagcgct
     3001 tcgcgacgcg cggggcgaat cgctttgctc cggccgactg gcacgacgga ccgcatggca
     3061 tgccgctgct gcatggctgc gttgccaccc tgatcgttcg caaccgcagt cgccatcccg
     3121 aaggcgacca cgtcattctg gtcggcgagg tggtgcaatt cgatgcgccg ggtggcacgc
     3181 cgctggtgtt tcacgacggc cgttacttcg ctagtgccgt cgaggagaag ctgccgcgtg
     3241 cgctgcgcac gccgtggaga tgatccggcc gggcctgcac gtcgcgccgg cgtcggatgc
     3301 cgagttggca acggagccac attgaccgca agaagcggat gggccgttga ttgcggtccg
     3361 tcgacaatgg ctgcatgaaa cgccgcgacc tgtccgacct cgccctgctc gccgcactct
     3421 ggggcgcgag cttcctgttc acccgcatgg cggtgccggc cttcggcgcc ttcgcactcg
     3481 ccgaactgcg cgtagcgatc gcggcgctga tgctgctgcc gctgctggcg ctgcgcggcg
     3541 acttctcgga gttgcgggca gcgccggcac ggttcgtcct gctcggcgcg gtcaacaccg
     3601 cgattccgtt cgcgctgttc gcgtgggcgg tcctgtcgat cacggccggc ctggcatcga
     3661 tcctgaatgc cactgcgccg ttgttcacag cactggtcgc ctgggtatgg ctgcgcgagc
     3721 ggctgacact gctgcagtgg gtcggcatgg ggatcggttt cgcaggcgtg ctgtggctgg
     3781 cgggcgcgaa ggccgggttc ggcggcgaca gcagcggctg ggccatcatt gcggcgctgg
     3841 tggctacgtt ctcctatggc gtttcggcca gcgtggcgaa gcgcaagttc gtcggcgtta
     3901 gacccctggc ggtggccgcc ggcagccaga ccgcggctgc gctcctgctg ttgcctttcg
     3961 cgatcgccac ctggccgtct gagccgcccg ccgtgatcga ctgggccgcc gcgaccgcgc
     4021 tcggcgtgct gtgcaccggc atcgcctaca tcctgtactt ccggctgatc gcccgggtcg
     4081 gcccagcgac ggcgatgacc gtgacctacc tcattccggc ctttgcgatg ctatggggcg
     4141 ggatctttct cgccgagaca gtcactccga cgatgctgat cgggtgcgca atcatccttg
     4201 ccggcacagc gctcgccacc ggcacactcc ggccacgacg atctgcgtcg acctctgcag
     4261 gaagttcgtg acgccattgc gctgacagcg ctgcagtgga gttgcaccgc gtgagcaggc
     4321 cctagaatcg cgccatgctg cgactctggg acatctcgcc gacaatcggg cccggtgttc
     4381 cggtgttccc cggcgacacc cctttcgcgc agcgctggac ctggacgctc gacgtccgtt
     4441 gccccgtcaa cgtatccgag atcacgctgt caccgcacac cggggcgcac accgacgcac
     4501 cgctgcacta cgccgccgac ggcgccgcga tggccgaggt gccgatccag atctatctcg
     4561 gcccatgccg cgtcgtgcac gccatcgacg tcgggtcgct catcgaaccg aagcatctgc
     4621 tcggtgacct cgacgacgcg cccgagcgag tgctgatccg cacgtacctg caggccccgt
     4681 tgcaggtatg ggacccagcg ttttcggcac tcgcgccctc gaccatcgat gcattgcatg
     4741 cgcggggcgt gcgcctcgtc ggcatcgaca ccccgtcgct cgacccggcc gacagcaaat
     4801 cgcttgacag ccaccagcgt gtgcgtgcgc acggcatggc catcctcgaa ggcatcgtgc
     4861 tcgacgacgt tccggccggt gactacgaac tgatcgccct gcccctgaaa atcgccggct
     4921 gcgatgcgag cccggtacgc gccgtgctcc gggagttgcc ggcgtgaccc gcgatgaggc
     4981 gctggcccgc gacgccgccg acccgctcgc ggcgcatcgg cgcggcttct gtctgccgga
     5041 gggcgtgatc tatctcgacg gcaactcgct cggggccctg ccactgtcga ccagtgcccg
     5101 agtgcgcgag gtcatcgagc gcgagtgggg cggaggattg atccggtcct ggaacactgc
     5161 gcggtggacc gacctgccgc gccgcgtggg cgccaaaatc gcgcgactga ttggcgcgaa
     5221 agcgcacgag gtcatctgcg cggactcgac gtcgatcaat ctcttcaagg tgctggcggc
     5281 cgcgttgcac ctgcagacgg cacggcccca ggtctcgatt gcggatcgcc gcgtgatcct
     5341 gtccgagcgc ggaaacttcc cgaccgacct ttacgtggcg caaggactca cgcaattgct
     5401 cggcgatcgc catgaactgc ggctggtcga taccgacgag gtgcacggcg ccatcgacga
     5461 aacggtggcg gtcgttctcc tgactcacgt gaacttcaag tcgggagcca tgcacgatct
     5521 cgccgcactc acgcgccggg cgcacgaggc cggtgcactc gttgtgtggg atcttgccca
     5581 ttcggcgggt gcagtgccag tcgacctgga cgcagcgcaa gcggacttcg ccgtggggtg
     5641 cggctacaaa tacctcaacg gcgggccggg tgcaccggcc ttcgtctatg cagcggcgcg
     5701 ccacctcgag gcactggccg gcgcgaccta tgcgcagccg ctgaccggct ggttcgggca
     5761 ccgcacgccg ttcgagttcg tcacccatta cacgccggcc gagacgatcg atcgcttcgc
     5821 tgtcggcacg ccgccgatca tcgcgctggc ggcactcgac gccggcgttg acaccgtgct
     5881 tgccgccggg atagacgcgt tgcgggcgaa gtcggtcgcg ctgaccgacg ccttcatcgc
     5941 actcgtcgag cgcgactgca agggcgtgac gctcgcgaca ccgcgtgcag ccgggcagcg
     6001 cggcagccag gtgtgctttg cgcatgccga cgccttcgcc gtcatgcagg ccctgatcga
     6061 gcgcggcgtc attggcgact ttcgcgcgcc cgacatcctg cgcttcggct tcgcgccgct
     6121 ctacgtccgc catgtcgatg tcttcgacgc ggcgcaggcc ctggccgatg tgctgcggtc
     6181 cggcgcatgg cgcgtggagc gcttccagca ccgggctatg gtcacatgag tcgtcccgcc
     6241 gatcccccgg cgatgcgcta cggcgactac ctggcgctcg acgccatcct cgatgcacag
     6301 catccgcgtt cggaggatcc gaacgagctg ctgttcatcg tgcagcacca gaccagcgaa
     6361 ctgtggatga aacttgcgct gcacgagctg cgcaccgcgc aggccgcgat tgcggcatcg
     6421 gacctgccgc ccgcgttcaa gatgctggcg cgcgtctcgc gcatcatgga acagctggtg
     6481 cacgcctgga acgtgctcgc cacaatgact ccgagcgagt acagcgcgat ccgcccgttc
     6541 ctgggcacga gttccggatt ccagtcgtgg cagtaccgcg agatcgagtt cctgctgggc
     6601 aacaagaacg ccgcgatgct gcggccgcat gacgatcgcc cagaaatgaa acagcggctc
     6661 gaggcggcct tcgccgcacc gtcgctgtac gacgatgcga tccgcctgct cgcgcgccgc
     6721 ggcttcgcca tcgaccccgc ggtgatcgaa cgcgacctga cgcagcggca tgccggcgac
     6781 cccagcgtcg aggcggcctg gctcgcgata taccgtgccc cgcgcgaaca ctgggaactg
     6841 tacgaactcg ccgaagaact ggtcgacctc gaggacgcgt tccggctgtg gcggttccgg
     6901 catgtgagca cggtggagcg catcatcggc ttcaagcccg gaaccggcgg caccagtggc
     6961 gtcggctatc tgcgcaagat gctcgaggtc gtgctgttcc cggagttatg gaaggttcgc
     7021 accgacctgt agatggcggt cctcgtgtac gcgacgcgct acgcgcgttg cacgtgcact
     7081 cgcaccggca cggttcgcac cgcgcgcgaa ccgctaaaca ctgaattgtc ggctgcgctt
     7141 ggggcgtagc ggggaatttt cgacagcacc tgcgtgctgt cgagcacgcg tagccggcgc
     7201 ggcctgcatg ccgcgcagtg gaccggccgc gcgctccgcc gatgttcaac ggcaccgcac
     7261 ctagccagct cccgccaggt gggacgaatt gacaaccgag gctacgcatc actcgacaaa
     7321 gccggggcca cccttggtag ctagaatcac cgctgtactg ttgttcacgt cccgtcgaag
     7381 tcgaggtccg catgaacgct cccgagaaat cccgctccgt gaaggccagc ttccagtggg
     7441 acgacccgct g
//
LOCUS       DATIRT010000462         3110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1063012, whole genome shotgun sequence.
ACCESSION   DATIRT010000462 DATIRT010000000
VERSION     DATIRT010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063012"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /gene="rplA"
                     /locus_tag="VLN25_10465"
     CDS             complement(<1..325)
                     /gene="rplA"
                     /locus_tag="VLN25_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430859.1"
                     /note="in Escherichia coli and Methanococcus, this protein
                     autoregulates expression; the binding site in the mRNA
                     mimics the binding site in the 23S rRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HSQ11018.1"
                     /translation="MTISKRSKTIHAKVDPQKHYAIADALALVKECANAKFDESVDVA
                     VVLGIDAKKSDQTVRGSVVLPAGTGKTKRVAVFAQGAKADEAKAAGADIVGFEDLAAK
                     VKEGFM"
     gene            complement(326..757)
                     /gene="rplK"
                     /locus_tag="VLN25_10470"
     CDS             complement(326..757)
                     /gene="rplK"
                     /locus_tag="VLN25_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HSQ11019.1"
                     /translation="MAKKIVGFIKLQVPAGKANPSPPIGPALGQRGLNIMEFCKSFNA
                     QTQGVEPGLPIPVVITAYADKSFTFVMKTPPATVLIKKAAKIDKGSPKPHTDKVGKIT
                     RAQAEDIAKAKMPDLTAAGLDAAVRTIAGSARSMGITVEGL"
     gene            complement(929..1489)
                     /gene="nusG"
                     /locus_tag="VLN25_10475"
     CDS             complement(929..1489)
                     /gene="nusG"
                     /locus_tag="VLN25_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013211203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HSQ11020.1"
                     /translation="MTTEAATSSSTKRWYVVHAYSGMEKSVARTLQERINRAGLQSQF
                     GQILVPVEEVVEVKGGKKSITERRMYSGYVLVEMDMTDEAWHLVKSTPKVTGFLGGTA
                     NRPSPLPQREVDEILLRMQEGAEKPRPKTLFEVGEMVRVKDGPFTDFNGNVEEVNYEK
                     SRLRVSVTIFGRSTPVELEFGQVEKV"
     gene            complement(1497..1877)
                     /gene="secE"
                     /locus_tag="VLN25_10480"
     CDS             complement(1497..1877)
                     /gene="secE"
                     /locus_tag="VLN25_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HSQ11021.1"
                     /translation="MTTQNVETVASKTDFAIVAVAVLAAAAGVLGFTFLTEQPTLVRV
                     GTLIGGLAAGIVIAWFSEPGKRFLAYARESYQETKRVVWPSRKETISTTGIVFGFVVL
                     MAIFLFVVDKSLEWFLYDLLLSWR"
     gene            complement(1924..1999)
                     /locus_tag="VLN25_10485"
     tRNA            complement(1924..1999)
                     /locus_tag="VLN25_10485"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1964..1966),aa:Trp,seq:cca)
     gene            complement(2049..>3110)
                     /gene="tuf"
                     /locus_tag="VLN25_10490"
     CDS             complement(2049..>3110)
                     /gene="tuf"
                     /locus_tag="VLN25_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HSQ11022.1"
                     /translation="ARAYDQIDAAPEEKARGITINTAHVEYETKNRHYAHVDCPGHAD
                     YVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIIVYMNKVDMVDDAE
                     LLELVELEVRELLSKYEFPGDDIPIVKGSAKMALEGDQGELGEQSIMKLADALDTYIP
                     TPERAIDGAFLMPVEDVFSISGRGTVVTGRIERGIVKVGDEIEIIGIKPTLKTTCTGV
                     EMFRKLLDQGQAGDNVGVLLRGTKREEVERGQVLAKPGSITPHTKFKAEVYVLSKEEG
                     GRHTPFFNGYRPQFYFRTTDVTGSIQLPAGTEMVMPGDNIQMDVALIAPIAMEEGLRF
                     AIREGGRTVGAGVVAKIVE"
BASE COUNT          526 a         1018 c          925 g          641 t
ORIGIN      
        1 ccatgaaccc ttccttgacc ttagctgcga ggtcttcaaa gccgacgatg tcggcgcccg
       61 ctgccttggc ctcgtccgcc ttggcaccct gcgcgaacac ggcgacgcgc ttggtcttgc
      121 cggtgcccgc cggcagcacg accgatccac ggactgtctg atccgacttc ttcgcgtcga
      181 tgcccagcac caccgcgacg tcgactgact cgtcgaactt ggcgttggcg cattccttca
      241 ccagagctag cgcatcggct atcgcatagt gcttctgtgg atcgaccttg gcgtggatcg
      301 tcttggagcg cttcgaaatc gtcatctaca ggccctccac cgtaatgccc atcgaacggg
      361 cgctgccggc aatcgtgcgc acagcggcat cgagcccggc ggcggtgagg tccggcatct
      421 tcgccttggc gatgtcctcg gcctgggcac gggtgatctt gccaaccttg tccgtgtggg
      481 gcttcggcga acccttgtcg atcttcgcgg ccttcttgat cagcaccgtt gccggtggtg
      541 tcttcatcac gaaggtgaag ctcttgtcgg cgtaggccgt gatcaccacc ggaatcggca
      601 gccccggttc cacgccttgc gtctgggcgt tgaacgactt gcagaattcc atgatgttca
      661 ggccgcgctg gccgagcgcg gggccgatcg gaggcgacgg attcgccttt cctgccggca
      721 cctgcaactt gataaagccg acgatcttct ttgccatgat ggctcctgtg ggttcaaacg
      781 ccgcgcggtt ctacgcacag ctccccggta atttcggctc gttgcctttg atccagtggt
      841 tggcgccgag ccgcctcaag ccaaccactc aaactaatcg ctgcgaagta tcgtgcgagc
      901 gcagcgagcg aaacttcgca gcgcctcgct agaccttttc tacctgcccg aactcgagtt
      961 cgaccggagt cgaacgaccg aaaatcgtca ccgacacacg caggcgactc ttctcgtagt
     1021 tcacttcttc cacgttgccg ttgaagtcag taaacggacc gtccttcacg cgcaccatct
     1081 cgcccacttc gaacagcgtc ttcggtcgcg gcttctcggc accttcctgc atgcgcagga
     1141 gaatctcgtc gacttcccgc tgcggcagcg gcgacgggcg gttggcggtg ccaccgagaa
     1201 agccggtcac cttgggcgtg ctcttgacca gatgccaggc ctcgtcggtc atgtccatct
     1261 caaccagcac atagcccgag tacatgcggc gctcggtgat cgacttcttg ccgcccttga
     1321 cctcgacgac ctcctcgacc ggcaccagga tctgtccgaa ctgcgactgc agcccggcgc
     1381 gattgatccg ctcctgcagc gtgcgcgcga cgctcttttc catgcccgaa taggcatgga
     1441 ccacgtacca gcgcttggtg ctcgaacttg ttgcagcttc agtcgtcatg tttgcctcag
     1501 cgccagctca acagcaggtc gtacaggaac cactccaggc tcttgtcgac gacgaacagg
     1561 aagatcgcca tcagtacgac gaagccgaaa acgatgccgg tagtgctgat cgtttccttg
     1621 cgcgacggcc agaccacgcg cttcgtttcc tgatacgact cgcgggcata ggcgagaaag
     1681 cgcttgcccg gctcggaaaa ccaggcgatc acgattccag ccgccagtcc gccaatcagt
     1741 gtgccgacgc ggacgagcgt cggctgctcc gtcaggaatg tgaacccgag gacgcctgcg
     1801 gcggccgcga gcaccgccac cgccacgatg gcgaagtcgg tcttgctggc tacggtctcg
     1861 acgttttgcg tcgtcatctg cagtgatctc ttcagcaccc gccactggcg gggttactcg
     1921 atttggcagg ggcagaggga atcgaacccc caaccttcgg ttttggagac cgacgctctg
     1981 ccagttgagc tataccccta ccggaactgg ccgatgcggc cgcgtggccg catagcgatc
     2041 tcgctttcct actcgacgat ttttgccacc acgccggcgc cgacggtgcg gccgccttcg
     2101 cggatggcaa agcgcagtcc ttcttccatg gcgatcgggg cgatcagcgc cacatccatc
     2161 tggatgttgt cgcccggcat caccatctcg gtgcccgcgg gcagctggat cgagccggtc
     2221 acatcggtcg tgcggaagta aaactgcggc cgatagccat tgaaaaacgg cgtgtgacgg
     2281 ccgccttctt ccttggagag cacatacacc tcggccttga acttggtgtg cggcgtgatc
     2341 gagccgggct tggccagcac ctggccgcgc tcgacttctt cgcgcttggt gccgcgcaag
     2401 agcacgccga cgttgtcacc ggcctggccc tggtccaaga gcttcctgaa catctcgacc
     2461 ccggtgcagg tggtcttgag cgtgggcttg atgccgatga tctcgatttc atcaccgacc
     2521 ttgacgatgc cgcgctcgat ccggccggtc acgacggtgc cgcgaccgga gatggagaac
     2581 acatcctcga ccggcatcag gaaggcgccg tcgatggccc gctcgggggt ggggatgtag
     2641 gtgtcgagtg cgtcggccag cttcatgatg gactgctcgc ccaactcgcc ctggtcgcct
     2701 tcgagcgcca tcttggcgct gcccttgacg atgggaatgt cgtcacccgg aaactcgtac
     2761 ttgctgagca gctcgcgcac ttcaagctcg acgagctcga gcagctcggc atcgtcgacc
     2821 atgtcgacct tgttcatgta gacgatgatg tagggcacac cgacctggcg cgccagcagg
     2881 atgtgctcgc gcgtctgcgg catggggcca tcagcagccg atacgacgag aatggcgccg
     2941 tccatctggg cggcaccggt gatcatgttc ttgacgtagt cggcgtgacc cgggcagtcc
     3001 acatgggcgt agtgccggtt cttggtctcg tactcgacgt gggcggtgtt gatggtgatg
     3061 ccgcgcgcct tttcctccgg cgccgcatca atctggtcat acgctcgcgc
//
LOCUS       DATIRT010000463         4285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_570181, whole genome shotgun sequence.
ACCESSION   DATIRT010000463 DATIRT010000000
VERSION     DATIRT010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570181"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..906
                     /locus_tag="VLN25_10495"
     CDS             145..906
                     /locus_tag="VLN25_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11023.1"
                     /translation="MDPILIAAVVFAFTFGGSLLGMWLRWKLPEHHLSDATKDTVKLG
                     IGMVATMTALVLGLVTASAKNSFDSVDSAVKHTAIDLLTLDRLLARYGPEAAPMRAAL
                     KATVGRKIEQVWPRDASKPVQVDPVQSGTAVEGLVEGIRALSPRDDAQRRLQSRALDL
                     GESLLTARWLAVADQSTSIPFAFLVVLLFWLMIIFASFGLFAPRNATVVVVLVLCALS
                     VAGAVFLVLEMDRPLEGLIKVSPDPLRYAFAKLNT"
     gene            1010..1552
                     /gene="hemG"
                     /locus_tag="VLN25_10500"
     CDS             1010..1552
                     /gene="hemG"
                     /locus_tag="VLN25_10500"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HSQ11024.1"
                     /translation="MANTLLLYSTNYGLSKSICERLQASLHRKGERAEVAPLVGHTVD
                     PKTFDAIVIGASIRHGKHSPAVLEFIRAHQSLLESKPSALFSVNLVARKPLKSTPQTN
                     PYLKRLVAQSPWKPKLLAVFAGELDYSRYGPIDKHMMRLVMWINKGPTDFSTKVQFTN
                     WDEVERFAGQVAQLVAGRSA"
     gene            1567..2082
                     /locus_tag="VLN25_10505"
     CDS             1567..2082
                     /locus_tag="VLN25_10505"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HSQ11025.1"
                     /translation="MRELLDRLANPVHLLVALACTWLLVSSPWIGMYQHMPDDTGAIN
                     LSHVALGLSLLPLGIVYFAACTLGGRWRLYFPWIAGQWGGLGRDLAGIFRAQRPGSEG
                     GGLFATIEGLLLLALLASAGTGTLWFSTQGTEAAVLWRGHHIFAARSFAVLMLLHVVA
                     VALHFVDLVRD"
     gene            2429..>4285
                     /locus_tag="VLN25_10510"
     CDS             2429..>4285
                     /locus_tag="VLN25_10510"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HSQ11026.1"
                     /translation="MTRSEHPAPAAAGLLLFSLLLISLLLPAHAQGVKKGKPPPEIYA
                     GIIVKYKSSSAAKATATSATTMRAVEERARVKVAASRQGAMGVAVYRFDKAMPAAEVR
                     AAAARMALDSNVEYAVPDQVMHLMQTTPNDMEYAARQWTLQAPPTIVGGANLPLAWQR
                     TTGSNTVVVAVVDSGIRPGHPDLAGRLLPGYDFISSDSLAALNYPANWNAADGNGRDA
                     DPTDPGDYLDDAVLAELPPNHGLQPRPSSWHGTHAAGTIGATGNNGVGIAGVDWSARL
                     LPVRVAGRNNVGTTSDIIDGIAWAAGLAVPGVPANANPARVINLSLGGPGPCSAAYQD
                     VIDRVRAVGAVVVAAAGNDGALVVSQPANCNGVIAVTAHSRSGDNASYANVGVQVALS
                     APGGGCGALALIDLSGAQDTFNQTCAGCHSIDSLRQQIMQRAPAGLTFAKSRAALDSA
                     LMGVDLGGQDTGDMAALAVGLNNTARNDLAGVISQVACSGPTDRVYAPINLGTTTPAA
                     EGYDNKVGTSTAAPHVSGVVALMLSLSPRLTADEVKSVLRSTARPHPAGTYCAMGNSD
                     CGSGLLDAAAAVQLVVNNRPIVTGSIQGSAAGVRPGMTFTLVGEVRAIGGRMA"
BASE COUNT          699 a         1407 c         1417 g          762 t
ORIGIN      
        1 gctcctcata gccgaggccg gtgaggacct gcgcgagttc tttcgtcagg ccgaggtcga
       61 tgaatggact attcattggc gcagcgtagc agcgcggcag gtcggctatc gtgtcgcctc
      121 cgtctcgcac aggccctcgc cgtcatggat cccatcctga tcgccgcagt tgtgtttgcc
      181 ttcacgttcg gcggttcgct tctgggaatg tggctgcgtt ggaagttgcc cgagcaccac
      241 ctgagcgacg cgaccaagga cacggtaaag ctcggcatcg gcatggtggc tacgatgacc
      301 gcactcgtgc tgggcctggt gaccgcctct gcgaagaact ccttcgactc cgtggactcc
      361 gcggtcaagc acacggcgat cgacctcctc acgctcgacc gactgctggc gcgctacggc
      421 cccgaagccg cgcccatgcg cgccgcgttg aaggcgacgg tcggccgcaa gatcgagcag
      481 gtgtggccgc gggacgcatc gaaaccggtg caagtcgatc cggtgcaatc ggggacggcg
      541 gtcgaaggcc tggtcgaagg catccgcgcg ttgagcccgc gcgatgatgc ccagcggcgc
      601 ctgcaatcgc gagcgctcga tctcggcgag tcgctgctga ctgcgcgctg gctggcggtg
      661 gctgaccaat ccacgtccat cccgttcgcg tttctcgtgg tgctgctctt ctggctgatg
      721 atcatcttcg cgagcttcgg actatttgcc cctcgcaacg caaccgtggt ggttgtgctt
      781 gttctgtgcg cgctttcggt tgccggtgca gtgttcctgg tgctggagat ggatcggccg
      841 cttgaaggtc tgatcaaggt ctcgcccgat ccgctgcgct acgcattcgc gaagctgaac
      901 acgtagtgcg ggcagcctcg tcgcggtcgc gactgatctt ggtcatcgta tcgccgcacg
      961 ccgattgcta ggctcctgcg cgtccgactc ccgacccaac gagtattccg tggccaacac
     1021 cctgctgctt tactccacca actacggctt gtcgaagagc atctgcgagc gcctgcaggc
     1081 gagcctgcac cgcaagggcg agcgcgccga ggtcgcaccg ctggtgggcc ataccgtgga
     1141 tccgaagacg tttgatgcga tcgtcatcgg tgccagtatt cgccatggca agcacagtcc
     1201 tgcggtgctg gaattcatcc gtgcccacca gtcgctgctg gagtcgaagc cgagtgcgct
     1261 cttttcggtc aacctggtgg cacgcaagcc gctgaagagc acgccgcaga ccaatcctta
     1321 tctgaagcgg ctggtcgcgc aatcgccgtg gaagccgaag ctgctcgctg tctttgccgg
     1381 cgagctcgac tactcgcgct acgggccgat cgacaagcac atgatgcggc tcgtcatgtg
     1441 gatcaacaaa gggccgaccg acttttccac caaggtgcag ttcaccaact gggacgaggt
     1501 cgagcgcttc gccgggcagg tcgcgcaact ggtcgcgggt cgcagcgcct aggactcggc
     1561 aggccgatgc gcgaactgct ggaccgcctc gccaatccgg tgcatctgct ggtcgcgttg
     1621 gcctgcacgt ggctcctcgt cagcagccca tggatcggca tgtaccagca catgccggac
     1681 gacaccggtg ccatcaatct gagccacgtt gcgcttggac tgtcgctgct gccgctgggt
     1741 atcgtctatt tcgccgcatg cacactgggc gggcgctggc gcctgtactt cccatggatt
     1801 gccggccagt ggggtggcct gggacgcgac ctcgcaggca tctttcgcgc ccagcggccc
     1861 ggcagtgaag gcggaggcct gttcgcgacg atcgagggtc tgctgctgct ggcgctgctc
     1921 gcctccgccg ggaccggcac gctgtggttc tcgacgcagg gcacggaggc ggcggtgctc
     1981 tggcgtggcc atcacatctt cgccgcccgc agtttcgccg tgttgatgct gctgcatgtc
     2041 gtggccgtcg cgcttcactt cgtggatctc gttcgggact gaattttgag gcggacgtat
     2101 cggtgcaggg cggccgtgtc atgcccgacc gaagtgcgta ccgaccccgt tcggcctagc
     2161 gctcactccc agttcaaacg tccggattag accgttcagt ttcacagaag caggtcgttg
     2221 cgttctatcg tgcacgcaat accgttgcat ctgtgcgagg tgtgttgcgc tcactccgcg
     2281 ctcgtgccgc gcccggattc gcgggcgttc gcaacgacga aatcaaacca ggaacaacgg
     2341 tcttgctagc tcagggcgtt gacgggcgtg tagtgcgccg agttcgacgc agcgattagg
     2401 ccgaatgagg cacgaaatga atgtatcgat gacacgtagc gaacatcctg caccggcggc
     2461 cgcgggcctg ctgctattca gcctgcttct cataagcctg ctgttgccgg cccacgcgca
     2521 gggcgtaaag aagggcaagc cgccacctga gatctatgcc ggcatcatcg tcaagtacaa
     2581 gtcgagctcg gcagccaagg cgacagccac cagtgcgacg acgatgcgcg cggtggagga
     2641 acgcgcgcgg gtcaaggtcg ccgcgtcgcg ccagggcgcg atgggcgtcg ccgtgtatcg
     2701 cttcgacaag gcgatgccgg cggcggaagt gcgcgccgcc gcggcccgga tggcactcga
     2761 ctcgaacgtc gaatacgccg tgccggatca ggtgatgcac ctgatgcaga cgacaccgaa
     2821 cgacatggaa tacgcggcca ggcagtggac gctacaggcg ccccccacga tcgtcggcgg
     2881 agccaacctg ccgcttgcat ggcagcgcac gacgggttcg aacaccgtcg ttgttgccgt
     2941 cgtcgacagc ggcatccggc cgggccaccc ggacctggca gggcggttgc tgcccggcta
     3001 cgacttcatc agcagcgatt cgctcgctgc gctcaactat ccggccaact ggaacgcggc
     3061 agacggcaat ggtcgcgatg ccgatcccac cgacccgggc gactacctcg acgatgccgt
     3121 gctggccgag cttccaccga accacggcct gcaaccgcgg ccaagctcct ggcatggcac
     3181 gcatgcggct ggcacgatcg gcgcgaccgg caacaacggc gtggggatcg cgggcgtcga
     3241 ctggagcgcc cgactcctgc cggtacgggt ggctggccgc aacaatgtcg gcacgacgtc
     3301 ggacatcatc gacggcatcg cctgggcggc gggtctcgcc gtgccggggg tgcccgcgaa
     3361 tgccaacccg gctcgcgtca tcaatctcag tctcggcggc cccggaccct gcagcgccgc
     3421 ctaccaggat gtcatcgatc gcgtgcgtgc cgtcggtgcg gtggtcgtag cggcggccgg
     3481 caacgacggc gcgctggtcg tctcgcagcc ggccaactgc aacggcgtca tcgcggtgac
     3541 ggcgcactcc cgcagtggcg ataacgcgag ctacgcaaac gtcggagtgc aggtcgcgct
     3601 cagcgcaccg ggcggcggct gcggtgccct cgcgctgatc gatctttcgg gcgcgcagga
     3661 cacgttcaac cagacctgcg ccggctgcca ctcgatcgac tcgctgcgac agcagatcat
     3721 gcagcgggcg ccggccggcc tgactttcgc gaagtcacgt gccgcgcttg attcggcgct
     3781 catgggtgtc gacctcggcg ggcaggacac cggggatatg gctgcactcg cagtgggact
     3841 caacaacacc gcgcgtaacg atctcgccgg cgtcatctcg caggtggcgt gctccggtcc
     3901 gaccgaccgg gtgtacgcgc cgatcaacct cggaacgacg acgccagcgg ccgagggcta
     3961 cgacaacaag gtcggcacca gtaccgccgc gccgcacgtc agcggcgtgg tcgcgctgat
     4021 gctgtcactg agcccgcgcc tgaccgccga cgaagtcaag tccgtgctgc ggagcacggc
     4081 gcgcccgcat cccgcgggca cctactgcgc gatggggaac agcgattgcg gttccggcct
     4141 gcttgacgcc gccgcggcgg tccagctcgt cgtgaacaac cggcccatcg tgacgggttc
     4201 gatccagggc tctgcggcgg gtgtgcgacc aggcatgaca ttcacgctcg tgggagaagt
     4261 cagggccatt ggcgggcgca tggct
//
LOCUS       DATIRT010000464         1932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1554949, whole genome shotgun sequence.
ACCESSION   DATIRT010000464 DATIRT010000000
VERSION     DATIRT010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554949"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..106
                     /locus_tag="VLN25_10515"
     CDS             <1..106
                     /locus_tag="VLN25_10515"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653769.1"
                     /note="heat shock protein involved in degradation of
                     misfolded proteins; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HSQ11027.1"
                     /translation="HTDFPPADIVKKALTIAGDLCIYTNQSHTIESLD"
     gene            298..1629
                     /gene="hslU"
                     /locus_tag="VLN25_10520"
     CDS             298..1629
                     /gene="hslU"
                     /locus_tag="VLN25_10520"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HSQ11028.1"
                     /translation="MTPREIVHELNKHIVGQDRAKRAVAVALRNRWRRQQVPDPLRTE
                     ITPKNILMIGPTGVGKTEIARRLARLADAPFIKVEATKFTEVGYVGRDVDSIIRDLVD
                     ISVKQTRESEMRKVTTRAQDAAEDRVLDILVPPSRDVGFAPATRDDENVTRQKFRKKL
                     REGELDDKEIDIEVSAVSPSLEVMAPPGMEELTQQIQSMFANMGRERRKPRRMRVADA
                     MKALTDEEAARLVNDEDLKSRAVANAESNGIVFIDEIDKIASRAELQGADVSRQGVQR
                     DLLLLVEGTTVNTKYGMVRTDHILFIASGAFHMAKPSDLIPELQGRFPIRVELDSLTV
                     DDFDRILKSTDASLTQQYQALLGTDGCVLEFTDDGIHRIAEIAFAVNEKTENIGARRL
                     YTVIEKLLEDVSFEVGSGVAGGTAPIRIDAAYVDTRLADLARNEDLARYVL"
     gene            1760..>1932
                     /locus_tag="VLN25_10525"
     CDS             1760..>1932
                     /locus_tag="VLN25_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HSQ11029.1"
                     /translation="MNLLLFLLGLGALVAGASLLVRGAAKLALSFGISPLVVGLTIVA
                     FGTSAPEMAVSIGA"
BASE COUNT          361 a          616 c          620 g          335 t
ORIGIN      
        1 ccacaccgac tttccgcccg ccgacatcgt caagaaagcg ctcacgatcg ccggtgacct
       61 gtgcatctat accaaccaga gccacaccat cgaatcgctg gactgaccgg caacacgccc
      121 cgtcagctga tgcgtcagcc atccgtgaca tctccccgcc gcggccgagg gccgcgccca
      181 gtggtttgaa cagcgcgcgt acgcgcggcc gttccgaagg gagcgcgcct aactcgagca
      241 ctgcgagtgc acgtgcgacc gcatagcggt cgcgtacacg agcagtgagt ttttatgatg
      301 acccccagag aaatcgtcca cgaactgaac aagcacatcg tcggccagga ccgagccaag
      361 cgcgcggtcg ccgtggcgct gcgaaaccgc tggcgccgcc agcaggtgcc tgatccgctg
      421 cgcaccgaaa tcacaccaaa aaacatcctg atgatcggtc ccacaggcgt gggcaagacc
      481 gagattgcgc gccggctggc gcggctggcc gacgcgccct tcatcaaggt cgaggccacc
      541 aagttcacgg aggtgggcta tgtcggccgt gatgttgact cgatcatccg cgacctcgtc
      601 gacatttcgg tgaagcagac acgcgagtcc gagatgcgca aggtcaccac gcgcgcacag
      661 gacgctgccg aggaccgcgt tctggacatt ctggttccgc cctcgcgcga tgtcggcttc
      721 gctcccgcga ctcgcgacga cgagaacgtg acgcgccaga agtttcgcaa gaagctgcgc
      781 gaaggcgaac tcgacgacaa ggagatcgat atcgaggtca gcgccgtgtc gccgtcgctc
      841 gaggtgatgg ctccgccggg catggaggag ctgacccagc agatccagtc gatgtttgcc
      901 aacatgggcc gcgagcgccg caagccgcgg cgcatgcgcg ttgccgacgc gatgaaggcg
      961 ctgaccgacg aggaagcagc caggctcgtc aatgacgagg atctgaagtc acgcgctgtc
     1021 gccaatgccg aatccaatgg catcgtcttc atcgacgaga tcgacaagat cgcgtcgcgt
     1081 gcggaactgc agggtgcgga cgtgtcgcgg cagggcgtcc agcgcgacct gctgctgctg
     1141 gtcgagggca caactgtcaa taccaagtac gggatggtca ggaccgacca catcctgttc
     1201 atcgcttccg gggcgtttca catggccaag ccctcggacc tgattcccga actgcaggga
     1261 cgatttccga tccgcgtcga gctcgattcg ctgacggtgg acgacttcga tcgcatcctg
     1321 aagtccaccg atgccagcct gacgcagcag taccaggcgc tgctgggcac tgacggctgt
     1381 gtgctcgagt tcaccgacga cggcatccat cgcattgcgg agatcgcctt tgcggtcaac
     1441 gagaagaccg agaacatcgg tgcgcgccgg ctgtataccg tgatcgagaa actgctcgag
     1501 gatgtgtcgt tcgaagttgg aagtggcgtg gcgggcggca cggctccgat ccgcatcgat
     1561 gcggcttacg tcgatacacg gctggccgat ctcgcgcgca atgaggatct ggcccgctac
     1621 gtgctgtagg gcatcgtcac agcgctgcgc ggcctgcgcc aggtcgcaat gatcccgagc
     1681 ggggcgtgac cgtgcactga gcggcgcctc ctatacttgc actgctggct gggcggtgcc
     1741 cggcatctcg tcgggcacca tgaacctgct gctgtttctg cttggccttg gcgcgctggt
     1801 cgcgggcgct tcactgctgg tgcgcggggc tgcaaagctc gcgctgtcct tcggtatctc
     1861 gcctctggtg gtcgggctga cgatcgtggc cttcggcacc agcgctccgg aaatggcggt
     1921 gtcgatcggt gc
//
LOCUS       DATIRT010000465         3177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_473395, whole genome shotgun sequence.
ACCESSION   DATIRT010000465 DATIRT010000000
VERSION     DATIRT010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473395"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VLN25_10530"
     CDS             complement(<1..597)
                     /locus_tag="VLN25_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018314692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HSQ11030.1"
                     /translation="MPDLFDNPMGLMGFEFVEFASPTPNVIEPLFEKLGFSLVARHRS
                     KDVVLYRQGDINFIINREPRSLAGYFAAEHGPSACALAFRVRDSHKAYARALELGAQP
                     IEVATGPMELRLPAIRGIGGAALYLIDRFEDGKSIYDIDFEFLPEFQSILDRHPKGHG
                     FSIIDHMTHNVYRGRMAFWGSFYERIFNFREIRYFDIKG"
     gene            complement(753..1112)
                     /locus_tag="VLN25_10535"
     CDS             complement(753..1112)
                     /locus_tag="VLN25_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11031.1"
                     /translation="MATLLQRATSLLTDAANTALAPASRHSLAYDAAFALATAALRMR
                     GYRADAARGNRSIVFQTLPHTVGAAAELWSALSAAHDRRNALEYSAALAPTQTEVADL
                     LLQVRSLDRLVRKAAGR"
     gene            complement(1132..1773)
                     /locus_tag="VLN25_10540"
     CDS             complement(1132..1773)
                     /locus_tag="VLN25_10540"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HSQ11032.1"
                     /translation="MPSTSIADALFGKTQQAVLGLLFGQPERSFYLRELVTAAGGGAS
                     QVQRELAQLSAAGLVTREPRGNQVWFKANATSPVFGELKSLITKTTGIADVVRATLEP
                     FSGQITAAFIYGSVARGEHDSDSDVDLMVVGSLPPSRLAPAQLTLRERLGRPVKAVVY
                     TAAELKQHVRQGEPFLTNVLRQPKIWLIGSDAEIGVPNEQGTRQPRRRKATQV"
     gene            1912..2739
                     /locus_tag="VLN25_10545"
     CDS             1912..2739
                     /locus_tag="VLN25_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HSQ11033.1"
                     /translation="MRLPTLHLFLVALLAVCGTALAQTPTSAEPPAGVFLEDLTSPEL
                     AAAIRAGKTTVLVPIGGTEQNGPHMVLGKHNVRVKVLVGRIARELGNALVAPVIAYVP
                     EGGVNPPTGHMRYPGTITVPADVYRKVLEAAARSLRLAGFRNIVFLGDSGDYQRDNEI
                     VARQFDREWAGTPVRVVAIVEYYRAATIGFAAALRQRGYSDAEIGVHAGLADTSLALA
                     IDPGLVRPEHPRTPQIGPPLPGVSGDPRRATPELGKAGVDMIVSQSVAAIRKAVGPR"
     gene            2858..>3177
                     /locus_tag="VLN25_10550"
     CDS             2858..>3177
                     /locus_tag="VLN25_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11034.1"
                     /translation="MSVAQAPAQAPAAAPAAPAVSTVPGMPPVADPTNLYSETTAAKM
                     SPALANDLPRVYVPHVQSNDVYVIDPATFTVVDKFKVGLNPQHVVPSWDLRTLWVANN
                     AENTT"
BASE COUNT          553 a         1021 c         1044 g          559 t
ORIGIN      
        1 gcccttgatg tcgaaatagc ggatctcgcg gaagttgaag atgcgctcgt agaaactgcc
       61 ccagaacgcc atgcgcccgc ggtacacgtt gtgcgtcatg tgatcgatga tgctgaaacc
      121 gtggcccttc ggatggcgat ccaggattga ttgaaactcg ggcaggaact cgaagtcgat
      181 gtcgtagatg ctcttgccgt cctcgaagcg gtcgatcagg tacagcgccg cgccgccgat
      241 gccgcggatc gccggcaggc ggagctccat cggcccggtc gccacctcga tcggctgcgc
      301 gccgagctca agcgcacgcg cgtacgcctt gtgcgaatcg cgtacgcgga acgcaagcgc
      361 gcaggcgctc ggcccgtgct cggccgcgaa gtagcccgcc aggctgcgcg gctcgcggtt
      421 gatgatgaaa ttgatgtcgc cttggcggta tagcaccacg tccttgctgc ggtgacgcgc
      481 gaccaggctg aagccgagct tctcgaacag cggctcgatc acgttcggcg tgggcgaggc
      541 gaattcgacg aactcgaatc ccatcaagcc catggggttg tcgaagaggt caggcattgc
      601 cggctccagg tggccagggg ttcaacagca aagcatgcac gataccgcct ggccggtgga
      661 gggcgcagca gaccgaatgc ggtggctaaa tcgcctgtcg tgtagcgatc cgccgggtgg
      721 ttttgacgcg cgacactagc cggggaaaga gcctatcgcc cggcggcctt tcgcacgagc
      781 ctgtcgagac tgcgcacctg aagtagcaga tcggcgacct cggtttgcgt aggcgccaga
      841 gctgcgctgt attccagtgc gttgcgtcga tcgtgtgccg cggatagagc cgaccaaagc
      901 tcggcagcgg cgccgacggt gtgcggcagc gtctggaaaa caatcgaacg attgccgcgc
      961 gcggcgtcag cgcggtaccc gcgcatgcgc aacgctgccg tggccagtgc aaacgcggcg
     1021 tcgtaggcga gcgagtggcg gctcgccgga gcgagcgcgg tgtttgctgc atccgtcagc
     1081 aggcttgtcg ctcgctggag gagcgtcgcc acttctggcg aggccgccgt ctcagacttg
     1141 agttgccttg cggcgacgag gttgtcgagt tccctgctca ttcggcacac cgatttcggc
     1201 atccgatccg atcagccaga ttttcggttg ccgtagcacg ttggtgagaa aaggttcacc
     1261 ctggcgcaca tgctgcttca gttctgcagc ggtgtacacc acggccttga ccggccgtcc
     1321 gagccgttcg cgtagcgtga gctgggcggg tgccaggcga gacggcggca acgagccgac
     1381 gaccatcaga tcgacatcgc tatccgagtc gtgctcgccg cgcgccaccg aaccgtagat
     1441 gaaagcagcg gtgatctggc ccgagaacgg ctcgagtgtg gcgcggacca catcggcgat
     1501 gcccgtggtc ttcgtgatca gcgatttcag ttcgccgaac accggactgg tcgcattggc
     1561 cttgaaccag acttggttgc cgcgcggttc gcgcgtaacg agcccagccg ccgaaagctg
     1621 agcgagttcg cgctgcacct ggctcgcgcc gccaccggca gcagtcacca gttcccgcag
     1681 gtagaaactg cgctccggct ggccgaaaag caggcccagc acagcttgct gcgtcttgcc
     1741 aaacagtgcg tcggcgatgg aagtcgaggg catagattga accggaattc ggtacgaatg
     1801 tacctaaaaa cggtacgtgc ggcaactggt ttttgcggca accagtttca gagcagttac
     1861 ttgtcgcaac accgaactgg agttcgtcac cctgcagggt aaatttcgtc gatgcgcctc
     1921 ccgaccctgc acctctttct cgttgctctg ctcgccgttt gcggcacggc gctcgcccag
     1981 acgccgacca gcgccgagcc gccggccggc gtgttcctgg aggacctgac ctcgccggaa
     2041 ctcgccgccg ccatccgggc cggcaagacc acggttctcg ttccgatcgg cggcaccgag
     2101 cagaacgggc cgcacatggt cctcggcaag cacaacgtgc gcgtgaaggt gctggtcgga
     2161 aggatcgcgc gggaactcgg caacgcgctg gtcgccccgg ttatcgccta tgtgcccgaa
     2221 gggggcgtca accccccgac ggggcacatg cgttatcccg gaacgatcac ggtgccggcg
     2281 gacgtgtacc gcaaggtgct cgaagccgcc gcacgcagcc tgcggcttgc cggattccgc
     2341 aacatcgtgt ttctcggcga tagcggcgat taccagagag acaacgagat cgtggcccga
     2401 caattcgatc gcgaatgggc gggcaccccg gtgcgcgtgg tcgccatcgt cgagtactac
     2461 cgggcggcaa cgattggctt tgccgcggcc ctcaggcagc gcggctacag cgacgccgag
     2521 atcggcgtcc atgccgggct cgcggacacg tcgctcgcgt tggccatcga tccggggctg
     2581 gtgcgacccg aacacccgcg gaccccacaa attgggccgc cactgcccgg tgtttcgggc
     2641 gatccccggc gtgccacccc cgagctaggc aaggcgggtg tcgatatgat cgtgtcccag
     2701 tccgtcgcgg ccatccgcaa agcggtgggc ccccggtagt gcggtaggcc tcgagcttcc
     2761 gcagcccgga tctgcaactt caggagttgt tgtggccttc attgttcgcg ctcgttacct
     2821 gcctgtcgct ctgccgcttg cttttgcgtt ctcgacgatc agcgtcgctc aggctccggc
     2881 gcaagcgccg gcggcagctc ccgcggcgcc agccgtatcg accgtgcctg gcatgccgcc
     2941 ggttgccgat ccgacgaatc tgtacagcga gaccacggcc gccaagatga gtccggcgct
     3001 cgcgaacgac ctgccgcgcg tgtacgtgcc gcacgtccag tcgaacgacg tgtacgtgat
     3061 cgacccggcg acgttcacgg ttgtcgacaa gttcaaggtc ggcctcaacc cgcagcacgt
     3121 cgtgccgtcg tgggacctgc gcacgctgtg ggtggcgaac aacgccgaga acacgac
//
LOCUS       DATIRT010000466         3037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_964936, whole genome shotgun sequence.
ACCESSION   DATIRT010000466 DATIRT010000000
VERSION     DATIRT010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964936"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            263..1420
                     /locus_tag="VLN25_10555"
     CDS             263..1420
                     /locus_tag="VLN25_10555"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSQ11035.1"
                     /translation="MKSGSTPALPERRPVQLQAVGGAAHDDTARIDRRGESSTAGRLA
                     RELGWPHAVLLLALLAFPFVASPFITFQIGAQALVLGLIALSLTFLGGYGGMISLAQM
                     TVAGIAGYTIGVLGINGNEEINVNWPWWLIVPCALVLSMAAGAFIGWLSARTEGIYTI
                     MITLAIGVAFYYLVLQNYSVFNGFQGLQKVYPPVILGVNWRDPLPFYLLVLALSAAGY
                     LAIVYLVRTSFGIALQGIRDNPRRMASLGFNVVAHRVAAHAVAALLAGVGGVLFVWYN
                     GLIGPGSVGTSWLINILIIAVLGGMRHPIGPFLGALVFVLMQTFAIDVIDRERFNLVI
                     GSVFLAIVLFSPDGLLGLWERLRSGFSREKPDRAEPAGAVGSTVRLAGRRQ"
     gene            1432..2679
                     /locus_tag="VLN25_10560"
     CDS             1432..2679
                     /locus_tag="VLN25_10560"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSQ11036.1"
                     /translation="MRFSKRSFMRTLVGTAVAAAFATTALAQQGGSVKIGVLATLEGP
                     FAAGGADGMRGAELAIRERGGVVAGRKIEMIKASSDAKPDVAVNSARKLVEQDKVDIL
                     VGPLSGGEGIAVKDYSKTQPQVTFINGSSGAQATTLTNPSPNFFRFNTDGGQWMVGLG
                     KEAMKRGYKRVMVIAEDYSFPYSQVQGFMTEYCGAGGKVPVKAWVPLGGKDYSSVIAR
                     IPKEIDALVVVLGGADAVNFLTQYEAAGGDKPMIGGSITVSQDVLNYRGKRRDSLVGT
                     LSAGPLADSIDTPEYKAFVASYKKNFPDGYPSPGLFALNYYINMKAALDGLDAVKGDL
                     GDGQKKYRDVLAKMTLKTPVGDIKLDENRQAIGTTFITEVVKDNKGGLSNKVVGKTDN
                     VNQTLGLKPADFKMGSRDVPDCP"
     gene            2696..>3037
                     /locus_tag="VLN25_10565"
     CDS             2696..>3037
                     /locus_tag="VLN25_10565"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSQ11037.1"
                     /translation="MASVVELPLAAQPLSAALSGDALVLDRVTRQFGALRAIEDISFA
                     VPAGERRAILGANGAGKTTLFNAITGDFPPTAGRVQFFGEDITQLPPHERIRRGLRRT
                     YQSSLLFRELSV"
BASE COUNT          484 a          955 c         1018 g          580 t
ORIGIN      
        1 ctggcttatg cgcccaccta cagcgtggtg ttcacgttcg tgatcctggt gggggtgctg
       61 gcattccgtc cgcgcggact gatggggcgg gccgcgtgag cgcgcacggc cgctccacgg
      121 cgcggctggc aggccgcgcc ggccacgcgg gcacagcagc acgcaggtgc tgctgtgaat
      181 tccccgctgc gcccaaagcg ctcatctcgc agcgcgcagc gcggtgggcg ttccagtgag
      241 cgccgcgcgc tggcgacagg cgatgaagtc gggatcgacg cccgcgttgc cggaacggcg
      301 tcccgtgcag ttgcaggccg tcggcggcgc ggcgcacgac gacactgcgc ggatcgatcg
      361 tcgtggcgaa tcttcgacgg ccggccgcct cgcgcgcgaa ctcggctggc cgcatgcggt
      421 cctgctgctg gcgttgctcg cgtttccctt tgtcgcctcg ccattcatca cgtttcagat
      481 tggcgcgcag gctctggtgc tcggcctgat cgcgctgtcg ctgacgttcc tggggggcta
      541 cggcggcatg atttccttgg cgcagatgac ggtcgccggc atcgctggct acacgatcgg
      601 ggtgctgggc atcaacggca acgaggagat caacgtcaat tggccctggt ggctgatcgt
      661 gccgtgcgcg cttgtgttgt cgatggcagc cggtgctttc atcggctggc tgtcggcccg
      721 caccgagggg atctacacca tcatgatcac gctggcgatc ggcgtggcgt tctattacct
      781 ggtactgcag aactacagtg tcttcaacgg ttttcagggt ctgcagaagg tctatccgcc
      841 ggtgatcctt ggggtgaact ggcgcgatcc gttgccgttc tacctgctgg tgctcgcttt
      901 gtccgcggcc ggatacctgg ccatcgtcta cctggtacga acctcgttcg gcatcgcact
      961 gcagggcatc cgcgacaacc cgcgccgcat ggcgtcgctc ggattcaatg tcgtcgcgca
     1021 ccgggtcgcc gcgcacgcgg tggctgcatt gctggcaggt gtcggtggcg tgctgttcgt
     1081 ctggtacaac ggattgatcg gcccgggttc ggtcggcacg tcctggctga tcaatatcct
     1141 gatcatcgcg gtgctcggcg gcatgcggca cccgatcgga ccctttctcg gcgcgctggt
     1201 gttcgtgctg atgcagacct tcgcgatcga tgtcatcgac cgcgagcgct tcaatctggt
     1261 gatcggaagc gtgttcctcg ccattgtgct tttttcgcct gacggactgc tcggtctgtg
     1321 ggagcggttg cggtccggtt tctctcgcga gaagccggac cgcgccgaac ccgccggcgc
     1381 cgttggttca accgtgcggc tggctggccg cagacaataa aggagactgc aatgaggttc
     1441 agtaagcgga gtttcatgcg cacgctggtt ggaaccgcgg tagcggcagc gttcgcgacc
     1501 accgcgttgg cccagcaggg cggctcggtg aaaatcggcg tcctcgccac gctggaaggc
     1561 ccattcgccg ccggcggagc cgacggcatg cggggcgccg aactggcgat ccgcgagcgc
     1621 ggcggtgtcg tggccggccg caagatcgag atgatcaagg cctcgtcgga cgcgaagccc
     1681 gacgtcgcgg tcaattcggc gcgcaagctc gtcgagcagg acaaggtcga catcctggtc
     1741 ggaccgctct cgggcggcga ggggattgcg gtcaaggact attcaaagac ccagccgcag
     1801 gtgacgttca tcaatggctc ctcgggcgcg caggcgacga ccctgaccaa tccctcgccg
     1861 aacttcttcc gtttcaacac cgatggcggg cagtggatgg tcgggctcgg caaggaagcg
     1921 atgaagcgtg gctacaagcg cgtgatggtg attgccgagg actactcctt tccatactcg
     1981 caggtccagg gcttcatgac cgagtactgc ggcgccggcg gcaaggttcc ggtcaaggcc
     2041 tgggtgccgc tgggcggcaa ggattattcg tcggtcatcg cccgcatccc gaaggaaatc
     2101 gacgcgctcg tcgtcgtgct gggtggcgcc gacgcggtca atttcctgac gcagtacgag
     2161 gcggccggcg gcgacaagcc gatgatcggc ggatcgatca ctgtcagcca ggacgtgctg
     2221 aactaccgcg gcaagcggcg cgactcgctg gtcggcacat tgtcggccgg tccactggcc
     2281 gattcgatcg acacgccgga gtacaaggcc ttcgtcgcca gctacaagaa gaacttcccg
     2341 gacgggtatc cgagcccggg cctcttcgcg ctcaactact acatcaacat gaaggcggcg
     2401 ctcgacgggc tcgatgccgt caagggtgac ctgggcgacg ggcagaagaa gtaccgcgac
     2461 gtgctggcca agatgacgct gaagacaccg gtcggcgata tcaagctcga cgagaaccgt
     2521 caggccatcg gcaccacgtt catcaccgag gtggtcaagg acaacaaggg tggactttcc
     2581 aacaaggtcg tcggcaagac cgacaacgtc aaccagacgc tcggattgaa gccggccgac
     2641 ttcaagatgg gctcgcgcga cgtgcccgat tgcccgtaac gctcatcgtc gcgtcatggc
     2701 ttcggtagtc gaattgcctt tggccgcgca gcccctttcg gctgcgctgt cgggtgacgc
     2761 gctcgttctc gatcgcgtta cccggcagtt cggcgccttg cgcgcaatcg aagacatcag
     2821 ctttgccgtc ccggcgggcg agcggcgtgc gattctcggt gccaacggcg ccggcaagac
     2881 caccttgttc aacgctatca ccggcgactt tccgccgaca gctgggcgcg tgcagttctt
     2941 cggcgaggac atcacccagc ttcccccgca cgagcgcatt cgccgcggtt tgcggcgaac
     3001 ctaccagtcg tcgctgctgt tccgcgagtt gtcggtg
//
LOCUS       DATIRT010000467         4494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1653820, whole genome shotgun sequence.
ACCESSION   DATIRT010000467 DATIRT010000000
VERSION     DATIRT010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1653820"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1387
                     /locus_tag="VLN25_10570"
     CDS             <1..1387
                     /locus_tag="VLN25_10570"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HSQ11038.1"
                     /translation="LFSLDLSWLAPLAIVVGVVVYLSRRNERAGQIGRIVLGLGVMIL
                     ALQLVTAATAPLAHGAGVKTLFAQISGDLLLDVLIGAVFAVMCYSSLAVVLLVAMLAG
                     SNVLPSHVALPLVLGANLGSGILALLTTWRSDAVTRRVPLGNFVFKAVGVTLALTLLP
                     FVTPWLKQLQINPRDLVVVFHLAFNVMLALTFIQFTGRVAKLTERWLPDGRDKETASQ
                     PRYLDPLALATPPLALGNAAREAIRLADTVQDMLTGLLTVLRNNDSQLAEVLRKRDDE
                     IDRLYSAIKRYLAQISREALDTGESRRWTEIIQFTINMEHVGDIIERGLLDVAERKIR
                     HGLSFSDAGITEITDLHTRLVANLQLSVAVFLNGDLKSAQSLMSEKVAFRELEMRYAN
                     QHLLRLSDNTRQSIETSSLHLDLLADFKRINSLFCSVAYPILESAGVLSKTRLLGEQP
                     ALFHSNETRTT"
     gene            1388..1954
                     /locus_tag="VLN25_10575"
     CDS             1388..1954
                     /locus_tag="VLN25_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014615585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HSQ11039.1"
                     /translation="MALSLTDIERLFATKGARMYAGEPVTQLQHALQAAELAEREAAS
                     DELVLAAFLHDLGHLINDQGDTPTLRGIDDRHECVALPFLRGLYGEAVLQPIRLHVDA
                     KRYLCARGDGRLAGAEYWAALSADSKRSLELQGGIFSDAEAAAFIAQPHAADAVRVRL
                     WDDMAKSADAVTPSVDRYLALAERLSCR"
     gene            2045..3634
                     /locus_tag="VLN25_10580"
     CDS             2045..3634
                     /locus_tag="VLN25_10580"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HSQ11040.1"
                     /translation="MRSLPAAALLVLLVLAACSKTPDNASPAVSSAPAKNEIAWVRPA
                     AMSDVDSAFDRAKAGNQPVFLFWSAAWCPPCNQVKSTIFTRQDFIDKSRSFVPVYVDG
                     DTPSGQALGKRFSVGAYPTMVLLRADGSEITRLAGSVEPAKYMQVLEHGIAGGSSARE
                     ALAAAMAGTPVSAEDWRLLAFYSWETDEQQIVPKVDAPATLLKLAHSCPPEQREASSR
                     LVLQAVAASATAKGDVKPAADKADALASLQSILDRPELVREHYDLIAGYASQMVAYTT
                     APKSKAREQLVAQWNTALDRLVADATLSNAGRIWALGGKVDLARLDNKDGPLPKPLLV
                     LVREQVARADRDTTDINERQSVITSAGGVLSDAGLLDESDVLLSIELERSHSPYYYMS
                     GLASNARKRGTPESKVAAIEWARQAYETSKGPATRLQWGGAYVNYLLELAPADAVRIE
                     AAAARVISEAVEMPDAFSARSRRSLERLSERLQKWNKDDTHDKLLARLHEKMAPVCAS
                     DALDAADRSACAALFTPARRA"
     gene            3639..4478
                     /locus_tag="VLN25_10585"
     CDS             3639..4478
                     /locus_tag="VLN25_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006758700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HSQ11041.1"
                     /translation="MTRPRYSAPLTRAEESEFVPFAHELIAIAFSTLRRYFLSGTEVV
                     TKADASPVTLADRQAEEAMRALIARRYPDHGIVGEEHGIKAGSRYRWVLDPVDGTRAF
                     ITHCFLFGTLIALERDDGAGYRPVLGVIAHSAPGLALIGLRDRCTLYATDGSARPVRV
                     RRCDRLADATVLATTHWSTGEQRGAKNVHRVEQLVERARLYRTWGDCFGYFALATGGA
                     DLMLDPTLAHWDVAAIVPVIEAAGGVVSGWTGGDPLAEMSLIASAGDLHDEVLRLLHG
                     AGN"
BASE COUNT          750 a         1516 c         1450 g          778 t
ORIGIN      
        1 gctcttctcc ctcgatctgt cgtggctggc gccactggcg attgtcgtcg gcgtcgtcgt
       61 ctacctttcg cgccgcaacg agcgcgccgg ccagatcggc cgcattgtgc tgggcctcgg
      121 cgtgatgatc ctcgcgctgc agctggtcac cgctgccacg gcgccactgg cgcacggcgc
      181 gggcgtcaag acactgttcg cgcagatctc cggcgacctg ctgctggacg tgctgatcgg
      241 cgccgtgttc gcggtgatgt gctattcgag cctggccgtg gtcctgctgg tagcaatgct
      301 ggccggctcg aacgtgttgc cgtcgcatgt ggccctgccg ctggtgctgg gcgccaatct
      361 tggcagcggc atcctggcgc tgctgaccac ctggcggtcg gatgcggtta cgcgccgtgt
      421 accgctcggc aatttcgtgt tcaaggccgt cggcgtgacg ctggcgctga ccctgctgcc
      481 gttcgtgacg ccctggctga agcagcttca gatcaatcca cgcgacctgg tcgtggtctt
      541 ccacctcgcc ttcaacgtga tgctggccct gacgttcatc cagttcaccg gccgcgtcgc
      601 caagctcacc gaacgctggc tgccggatgg ccgtgacaag gaaaccgcgt cgcagccgcg
      661 atacctggac ccgctggcac tggccacgcc acccctcgcg ctcggcaatg cagcgcgtga
      721 agcgatccgc ctggccgata ccgtgcagga catgctgacc ggcctgctga ccgtgctgcg
      781 gaacaacgac tcccagctgg ccgaggtgct gcgcaagcgc gacgacgaga tcgaccggct
      841 gtactccgcc atcaagcggt accttgcgca gatcagccgc gaggcgctcg ataccggcga
      901 atcacggcgc tggaccgaga tcatccagtt cacgatcaac atggagcatg tcggcgacat
      961 catcgagcgt ggactcctcg atgttgccga acgcaagatc cgccacggcc tgtcgttctc
     1021 cgacgccggg atcaccgaga tcactgactt gcacacgcgg ctagtcgcca atcttcagct
     1081 cagtgtcgcc gtcttcctca atggcgacct gaagtccgcg cagtcgctga tgtcggaaaa
     1141 ggtggccttc cgcgagctgg aaatgcgcta cgccaaccag cacctgctgc gactttcgga
     1201 caacacgcgc cagtcgatcg agaccagctc gctgcacctc gacctgctgg cggacttcaa
     1261 gcgcatcaat tcactgttct gctcggtcgc ctacccgatc ctcgaatcag ccggagtcct
     1321 gtccaagacc cggctgctcg gcgagcaacc ggctctgttc cacagcaacg agacacgcac
     1381 tacctgaatg gcactttcac tgacagacat tgaacggttg ttcgcgacca agggtgcccg
     1441 catgtacgcg ggcgagccgg tgacccagct gcagcatgcg ctgcaagcgg cggaactcgc
     1501 cgaacgcgag gcagcgagcg atgaacttgt gctcgccgcc ttcctgcacg acttggggca
     1561 cctgatcaac gaccagggtg atacgcccac gctgcgaggc atcgacgacc ggcacgagtg
     1621 tgtcgctctg ccgtttctgc gcggactgta tggcgaggcc gtgttgcagc caatccgcct
     1681 gcacgtcgat gcgaagcgct acctgtgcgc gcgcggggac ggacggctgg ccggtgctga
     1741 atactgggcg gccctgtcgg ccgactcgaa gcgcagcctc gaactgcagg gcggcatctt
     1801 ctccgacgcg gaggccgcag ccttcatcgc ccagccgcac gccgccgacg cggtgcgcgt
     1861 tcgcctgtgg gacgacatgg ccaagtcggc cgacgcggtc accccttccg tcgatcgcta
     1921 tctcgcactc gccgaacgcc tcagttgccg ctaggttgcc gctgagttgc gcccgagggt
     1981 agccggcggg cgataaactg ccccatggcc ttcaaggcgc gcacaccttc ttaaaggaag
     2041 ttgcatgaga tccctgcccg ctgcggccct gctggtgctg ctcgtcctgg ccgcatgctc
     2101 gaaaacaccc gacaacgcca gcccggcggt ttcgtccgcg ccggcaaaga acgaaatcgc
     2161 ctgggtccga cccgccgcga tgagcgacgt cgacagcgct ttcgatcgcg cgaaggctgg
     2221 caaccagccg gtgttcctgt tctggtccgc cgcctggtgt ccgccgtgca accaggtgaa
     2281 gagcaccatc tttacgcgtc aggatttcat cgacaaatca cgctcgttcg ttccggtgta
     2341 cgtcgacggc gatacaccga gcggacaggc gctcggcaaa cgcttcagtg tcggtgcgta
     2401 tccgacgatg gtgttgctgc gcgcggacgg cagcgagatc acgcggctgg ccggctcggt
     2461 tgaaccggcc aagtacatgc aggtgctcga gcacggaatt gcgggcggat cgtcggcccg
     2521 ggaggcactg gccgcggcca tggccggcac gcccgtcagc gcggaggact ggcggctgct
     2581 ggcgttctat tcctgggaaa ccgacgagca gcagatcgtc ccgaaggtcg acgccccggc
     2641 cacgctccta aagttggcgc actcctgtcc gcctgagcag agggaagcgt cgtcgcgcct
     2701 cgtgctgcag gccgtcgccg cctcggccac cgccaagggc gacgtcaagc cggcggccga
     2761 caaggccgat gcgctcgcga gcctgcagag catcctcgac cgaccggaac tggtccgcga
     2821 acactacgac ctgatcgccg gctacgcctc gcagatggtg gcttacacga ccgcaccgaa
     2881 gtcgaaggcg cgcgagcaac tcgtcgctca gtggaacacc gcactcgaca gactggtggc
     2941 cgatgccacg ttgtcgaacg cgggtcggat ctgggcgctg ggtggaaagg tcgatctggc
     3001 ccggctcgac aacaaggacg gccctcttcc caagccgctg ctggtgctgg tgcgcgagca
     3061 ggttgcgcgc gccgatcgcg acaccaccga catcaatgag cgtcagtcgg tcatcacgag
     3121 cgcgggaggg gtgctgtccg atgccggctt gctcgatgaa tctgatgtcc tgctgtcgat
     3181 tgaactggag cgatcgcact caccgtacta ctacatgtcc ggtctggcct ccaacgcccg
     3241 caagcgtggg acacccgaaa gcaaggtcgc cgcgatcgaa tgggcgcgtc aggcctatga
     3301 gacctccaag ggtccggcga cgcgcctcca gtggggaggt gcctatgtca actatctgct
     3361 cgaacttgca cccgctgacg cggtgcggat cgaggcagcg gcagcgaggg tcatcagcga
     3421 agcagtcgag atgccggatg ccttttcggc acgcagcagg cgctcactcg agcgcctgag
     3481 cgaacgcctg cagaagtgga acaaggacga cacgcacgac aagctgctcg cgcgcctgca
     3541 cgagaagatg gcgccggtct gcgcttcgga cgcattggac gcggccgacc gcagtgcgtg
     3601 cgcggccctt ttcacgccag ccaggcgcgc ctgaaccggt gacacgcccc cgctacagcg
     3661 cgccgctgac gcgcgccgaa gagagcgaat tcgtcccgtt tgcgcatgag ctgatcgcca
     3721 ttgccttcag caccctccgc cgctacttcc tttccggcac agaagtcgtt accaaggccg
     3781 acgcctcgcc cgtgacgctg gcggaccgcc aggcggagga ggccatgcgc gcgctgatcg
     3841 cgcgccgcta cccggatcac ggcatcgtgg gtgaagagca cggcatcaag gcgggcagcc
     3901 gctaccgctg ggtgctcgat cccgtcgacg gcacgcgcgc gttcatcact cactgtttcc
     3961 tgttcggcac gctgatcgcg ctggaacgcg acgacggcgc cgggtatcgc ccggtactgg
     4021 gcgtgatcgc gcattcggct ccgggtctgg cgctgatcgg cctgcgcgat cgttgcacgc
     4081 tgtacgcgac tgacggttcg gcgcgaccgg tgcgtgtgcg ccgctgcgac cgcctcgccg
     4141 atgcgacggt gctggccacc acgcactggt caaccggcga gcagcgcggc gcgaagaatg
     4201 ttcatcgcgt cgaacagctt gtcgagcgcg cacggctcta ccgtacctgg ggcgactgtt
     4261 tcggctactt cgcactggcc acgggcggtg ccgacctgat gctggatccg acacttgcgc
     4321 actgggatgt cgccgcgatc gtgccggtca tcgaggcggc gggcggcgtt gtcagcggct
     4381 ggaccggtgg cgatccgctt gcagaaatgt ccctgatcgc cagcgccggc gatttgcacg
     4441 acgaagtgct gcgcctgttg cacggcgccg gaaactagct ttccggcgtt gatc
//
LOCUS       DATIRT010000468         7285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_571754, whole genome shotgun sequence.
ACCESSION   DATIRT010000468 DATIRT010000000
VERSION     DATIRT010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7285
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571754"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..126)
                     /locus_tag="VLN25_10590"
     CDS             complement(<1..126)
                     /locus_tag="VLN25_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HSQ11042.1"
                     /translation="MSGKRRVVVTGLGVISPLGNEVPSTWAALLEGRSGIGPITRF"
     gene            complement(160..402)
                     /gene="acpP"
                     /locus_tag="VLN25_10595"
     CDS             complement(160..402)
                     /gene="acpP"
                     /locus_tag="VLN25_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HSQ11043.1"
                     /translation="MENIEQRVKKIVAEQLGVNEADIKNESAFVEDLGADSLDTVELV
                     MALEDEFECEIPDEEAEKIRTVQQAVDYINSHLKKA"
     gene            complement(559..1320)
                     /gene="fabG"
                     /locus_tag="VLN25_10600"
     CDS             complement(559..1320)
                     /gene="fabG"
                     /locus_tag="VLN25_10600"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006410744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HSQ11044.1"
                     /translation="MNVFQSQCLMDEVALVTGASRGIGKAIAEQLARAGAVVIGTATT
                     AAGAAEIAERLAELGARGRGAVFDVRDAAATDELVDGIAKEHGKLSLLVNNAGITRDA
                     LAMRMKDDDWGAVLDTNLSGVFRLSRAVMRGMMKAKHGRIINITSVVGASGNAGQANY
                     AAAKAGVVGLTKALAREVGSRGITVNCIAPGFIDTDMTRALNEQQTQALLGQIPLGRL
                     GQPEEIGAAVVFLASPAASYVTGVTLHVNGGMYMN"
     gene            complement(1317..2267)
                     /gene="fabD"
                     /locus_tag="VLN25_10605"
     CDS             complement(1317..2267)
                     /gene="fabD"
                     /locus_tag="VLN25_10605"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HSQ11045.1"
                     /translation="MKLAFVFPGQGSQSVGMLSSFSDRPVVASVIDRASAALNQDMRS
                     LIADGPVEDLNLTVNTQPAMLVAGFAMYEAWRAAGGAEPALMAGHSLGEYTALTAAGA
                     LTLEDAVTLVRFRASAMQEAVPVGVGAMAAILGLSDDDVRRACAESAGSEVVEPVNFN
                     APAQVVIAGHKAAVERACEAAKRLGAKRALILPVSAPFHSSLLQPAATRLREYLMSVR
                     LVAPAVPVVNNVDVARESEAARIVDALARQAASPVRWVETVQSLAEQGITHVVECGPG
                     KVLAGLTRRIAPALESIAIYDDASLDAALATTANPGAAAE"
     gene            complement(2272..3279)
                     /locus_tag="VLN25_10610"
     CDS             complement(2272..3279)
                     /locus_tag="VLN25_10610"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HSQ11046.1"
                     /translation="MSARSGGAAQKTRHARIVGTGSALPARVVTNSELARELAGRGVE
                     TSDEWIVARTGIRQRYIAEAGVTSAELGTQAARAALAMADAPVETVDLIIVATSTPDQ
                     IFPSAACLIQARLGHPGCAAFDVQAVCSGFIYALTVAEAMLASGKHQRALVIGTEIFS
                     RILDWNDRSTCVLFGDGAGAVLLEASERPGILASELCADGSQAGILCTAGRVSHGAVQ
                     GHPFLTMDGQAVFKLAVSSLGSSAVDVLSAAGMTTDQLDWLIPHQANIRIMNAIARRL
                     GLPESKMIVTVDQHGNTSAASVPLALDAAVRDGRIRGGHHVLLQGVGGGFTWGSVLLR
                     W"
     gene            complement(3308..4387)
                     /gene="plsX"
                     /locus_tag="VLN25_10615"
     CDS             complement(3308..4387)
                     /gene="plsX"
                     /locus_tag="VLN25_10615"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HSQ11047.1"
                     /translation="MNIRIAVDCMGGDHGISVTVPAALHFLADEPGAMLLLVGRSAEI
                     ERAMGTASKGSASKGFVTRRIEVVHADEVIEMHDAPATALRSKRNSSMRVAINLVKEG
                     RADVVVSAGNTGALMAISRFVLKTLDGIDRPAIAGVIPNRGGGGTTMLDLGANVDCGP
                     DHLLQFAVLGSALVAAVEEKDRPTVGLLNIGEEMIKGNDIVKQAAELLRASPLNFFGN
                     VEGNDIYKGTTDVVVCDGFVGNVALKSSEGLANMLGDFMREEFKRDPLSKLMALAAYP
                     VLNRFRRRVDPRRYNGASLLGLRGLVLKSHGSADRLGFACALRRGFEEARHRVLERTV
                     AAMQPFVVQLEQMHGHRPEAAETDA"
     gene            complement(4507..4689)
                     /gene="rpmF"
                     /locus_tag="VLN25_10620"
     CDS             complement(4507..4689)
                     /gene="rpmF"
                     /locus_tag="VLN25_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HSQ11048.1"
                     /translation="MAVQQNKKSSSKRGTHRAHDFLTNPPTAIEPTTGEVHRRHHISP
                     NGVYRGKKVLQTKNDE"
     gene            complement(4755..5297)
                     /locus_tag="VLN25_10625"
     CDS             complement(4755..5297)
                     /locus_tag="VLN25_10625"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HSQ11049.1"
                     /translation="MTSRSHSYIDVFELARDKATIEGSLSLASLPRLAAALARPEGVL
                     RYRIQGQLDERGRPGAHMHLQGDLVLECQRCNEPYTFLLEREAQFRFVDGEEELNALP
                     IEDDEVDAIVGARRMDVSTWIEDEAILSLPLIPRHPWDDAACRPAAELGAAEEPSREP
                     TGREHPFAVLADLKPGSKAS"
     gene            5351..5944
                     /locus_tag="VLN25_10630"
     CDS             5351..5944
                     /locus_tag="VLN25_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007741742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HSQ11050.1"
                     /translation="MDSPRIVLASTSRYRRELLGRLRLTFDVSAPQVDESPLARETPG
                     ATALRLAVAKAREVHARHPGAIVIGSDQVAELDGSALGKPNQHEAALAQLELMQGREV
                     VFHTAVAVAGAELEALQVDNIPTYVTMRSLPRAALESYLRADEPYDCAGAAKIESLGI
                     ALVDRVRSDDPTALIGLPLIRLTAMLAAVGIAVLPVR"
     gene            5948..6649
                     /locus_tag="VLN25_10635"
     CDS             5948..6649
                     /locus_tag="VLN25_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HSQ11051.1"
                     /translation="MATLYLLPCAIAEGSELSVLPAQVIAIAQRLTYFLAENAKSARA
                     FLKAIGHPQPLRELQIIEIGHAPPAADIPAWLEPLIAGLDGALVSEAGCPAVADPGAE
                     IVAAAHQRGLRVQPLVGPSSLLLALMASGLNGQQFRFHGYLPVPAGDRTRRLVALERD
                     SRAGGTQLFIETPYRSPAMFDAIMTSCAPATRLSIAIDLTGPSEFVQTRTIAEWKRTH
                     RPPVERRPTVFLLLA"
     gene            complement(6646..7032)
                     /locus_tag="VLN25_10640"
     CDS             complement(6646..7032)
                     /locus_tag="VLN25_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HSQ11052.1"
                     /translation="MAAGDALIPICSPDALKDGARGVRFEVMFRGERAPAFAVRHRGI
                     AVAYLNRCAHVAMELDWQPGEFFEPDAEFLMCSTHGALYDPATGACRGGACAGHGGLR
                     PLTVVESATTVCWEPDHHTRPLDDSL"
     gene            complement(7011..>7285)
                     /locus_tag="VLN25_10645"
     CDS             complement(7011..>7285)
                     /locus_tag="VLN25_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HSQ11053.1"
                     /translation="FFASSRCADETNPKPHPAMLLELTEELAVPRARTVMIGDTTHDL
                     AMARSAGTASIGVTYGAHPREQLASYEPLGLAASVKELHRWLLATR"
BASE COUNT         1271 a         2462 c         2270 g         1282 t
ORIGIN      
        1 gaagcgcgtg atgggcccga tacccgaacg accttcgagc agtgccgccc acgtgcttgg
       61 cacttcattg ccaagcgggc tgatgacgcc aaggccggtg acgacgacgc gacgcttgcc
      121 tgacatcgaa cctctactga atgctgccag cggccccgtt caggccttct tgaggtgact
      181 gttgatgtag tcgacggcct gctgcacggt gcggatcttc tcggcttcct cgtccggaat
      241 ctcgcactcg aattcgtcct ccagcgccat cacgagctcg actgtgtcga gcgagtcggc
      301 accgaggtcc tccacaaagg ccgactcgtt cttgatatcg gcttcgttga ctccgagttg
      361 ctcggcgacg atcttcttga cgcgttgttc aatgttttcc atcgactcct cctgcctcat
      421 gtgtgttcgg ggtgcgccac gatctgcgca aggactgctg ccggtgcgca ccgccatgtt
      481 tttggccggc gaaaaaggcg cgcggattct aacagaggca cactgctcag cctgtgctgc
      541 gtgccacgac gagttcactc agttcatgta cataccgcca ttgacgtgca gcgtcacacc
      601 agtgacgtag gacgccgccg gtgaggcgag aaacacgacg gcagcaccga tttcctcggg
      661 ctgcccgaga cggcccagcg gaatctgtcc cagcaacgcc tgcgtctgct gctcgttcag
      721 ggcgcgcgtc atgtcggtgt cgatgaagcc aggcgcaatg cagttgaccg tgatcccccg
      781 gctgccgacc tcgcgcgcca gcgccttggt gagcccgacc acacccgctt tcgccgcagc
      841 gtagttggcc tgcccggcat tgccgctcgc tcccacgacc gaggtgatgt tgatgatgcg
      901 gccgtgcttg gccttcatca ttccgcgcat gaccgcccgc gagagccgga acacgcctga
      961 aagattcgtg tccagcaccg caccccagtc atcgtccttc atgcgcatcg caagcgcgtc
     1021 gcgcgtgatg ccggcattgt tcacgagcag cgacagcttg ccatgctcct tcgcgatgcc
     1081 gtcgaccagc tcgtcggtcg cggcagcgtc gcgcacgtca aagaccgccc ctcgtccgcg
     1141 tgcacccagt tccgccaggc gctccgcaat ttcggcagca ccggcggcgg ttgtggcggt
     1201 cccgatcacc acagcaccgg cgcgtgcaag ctgctcggcg atcgccttgc cgatgccgcg
     1261 cgacgcaccg gtgacgagcg ccacctcgtc catcaggcat tgcgactgaa acacgttcat
     1321 tcggccgcgg ctccagggtt ggccgtcgta gcgagtgcgg cgtcaaggct cgcatcgtcg
     1381 tagatcgcaa tactctccag cgccggcgca atgcgccgcg tgagcccggc gagtaccttt
     1441 ccgggtccac actcgacgac atgggtgatg ccctgctcgg ctagcgactg gaccgtctcg
     1501 acccagcgta ccgggctcgc agcctgccgt gccagtgcat cgacgatacg cgcagcctcc
     1561 gactcgcggg cgacatcgac gttgttcacg accggcaccg ccggagcgac cagacgaacc
     1621 gacatcaagt attcacgcag gcgcgtcgcg gccggctgca gcagcgacga gtggaacggt
     1681 gccgatacgg gcaggatcaa tgcgcgcttg gcgccaagcc gtttggcggc ctcgcaggcg
     1741 cgttccaccg cggccttgtg gccggcaatg acgacctgcg ccggggcatt gaagttaacg
     1801 ggttcgacga cctcgctgcc ggcgctctcg gcacaggcgc ggcgcacgtc gtcatccgac
     1861 agtccgagta tcgcggccat cgcgccaacc cccacgggca cggcttcctg catcgcggag
     1921 gcgcggaacc gcaccagcgt caccgcgtcc tcgagcgtca gtgcgcccgc cgccgtcaac
     1981 gccgtgtact cgcccagact gtgacccgcc atcagcgctg gctcggcgcc cccggcagcg
     2041 cgccaggcct cgtacatggc gaaacccgcc accagcatgg cgggctgcgt attcaccgtc
     2101 aggttcagat cttcgaccgg cccatcggca atcagggatc gcatgtcctg attcaacgct
     2161 gccgaagcac gatcgatcac cgaggcgacg accggacgat ccgaaaagct cgacaacatc
     2221 ccgacggatt gcgaaccctg ccctggaaac acgaatgcaa gcttcacttc atcaccatct
     2281 cagcagcact gaaccccaag tgaaccctcc gccgacacct tgcagcagta catggtggcc
     2341 gccacggatg cgaccgtccc ggaccgcggc atccagcgcc agcgggaccg acgctgccga
     2401 cgtattgcca tgctgatcga ccgtaacgat catcttggac tccggcagtc cgaggcggcg
     2461 cgcaatagcg ttcatgatcc ggatgtttgc ctggtgcggg atcagccagt cgagttggtc
     2521 cgttgtcatg ccggccgccg acaggacatc gaccgccgac gaccccaggc tcgaaaccgc
     2581 aagcttgaag accgcctgac cgtccatcgt gagaaacgga tgcccctgta ccgcgccgtg
     2641 gctgaccctc ccggccgtgc agaggatgcc ggcctgactg ccgtcggcgc acagttcgct
     2701 cgcgagtatg ccgggtcgtt cggaagcctc gagcaggacg gccccggctc cgtcaccgaa
     2761 cagcacacag gtggagcggt cgttccagtc gagaatgcgc gaaaagatct cggtgccgat
     2821 gaccagcgcc cgctggtgtt tgcccgacgc cagcatggcc tcggccaccg tgagcgcata
     2881 aatgaaacca ctgcagaccg cctggacgtc aaaagcggca cagcccggat ggcctaatcg
     2941 cgcctggatc aggcacgccg cactggggaa aatctggtcc ggcgtagagg tcgcgacaat
     3001 gatcaggtcg actgtctcca ccggcgcatc ggccattgca agcgcggcgc gggctgcctg
     3061 agttccaagc tctgctgacg tgacgccggc ctccgcgatg tagcgctggc ggatacccgt
     3121 gcgcgccacg atccattcat ccgacgtctc gacgccgcgt ccggcaagct cgcgggcaag
     3181 ctcggagttg gtcaccacac gcgccggcaa cgcggacccc gtgccgacga tgcgcgcatg
     3241 gcgggttttc tgtgccgctc ctcccgatcg cgcgctcacg gcctcgtcct tgagggagcc
     3301 gacgccatca tgcgtcggtc tctgccgctt caggtcgatg cccgtgcatc tgctcgagtt
     3361 gcacaacaaa gggttgcatg gcggccaccg tgcgctccag cacgcgatgg cgcgcctcct
     3421 cgaacccgcg acgcaacgcg cacgcgaagc cgaggcggtc ggccgagcca tggcttttca
     3481 acaccagccc gcgcaacccc agcaggctcg cgccgttgta gcggcgcggg tcgacgcgac
     3541 gccggaaacg attgagcacg ggataggcgg ccagtgccat cagcttcgag agcggatcgc
     3601 gcttgaattc ctcgcgcatg aagtcgccga gcatgttcgc caggccctcc gaggatttca
     3661 aagcgacgtt gccgacaaat ccgtcgcaga caacgacatc ggtcgtgccc ttgtagatat
     3721 cgttgccctc gacatttcca aagaaattga gcgggctcgc gcgcagcagt tcggcggcct
     3781 gcttgacgat atcgttaccc ttgatcatct cctcgccgat gttgaggagg ccgacggtcg
     3841 gacggtcctt ttcctcgacg gcggcgacaa gggccgatcc gagcacggcg aactgcagca
     3901 ggtgatcggg gccacagtcg acatttgccc caaggtcgag cattgtcgtg cctccgccgc
     3961 cgcggttcgg gatcacgccg gcgatcgccg gacggtcgat gccgtcgagt gtcttgagca
     4021 cgaacctcga gatggccatc agcgcacccg tgttgccggc actcacgaca acgtcggcgc
     4081 gcccttcctt gaccaggttg atcgcgacgc gcatcgacga gttgcgcttg ctccgaagcg
     4141 cggttgccgg cgcatcgtgc atttcgatga cttcgtcggc atgcacgacc tcgatgcgcc
     4201 gggtgacaaa acccttcgaa gcagacccct tggaggcagt ccccatcgcg cgctcgatct
     4261 ctgcgctccg gccgacgagc agcagcatgg cccccggctc gtccgcgagg aaatggaggg
     4321 ccgcgggcac ggtcacggaa atcccgtgat ccccgcccat gcagtcgacg gcgatacgga
     4381 tgttcaccgc gcaaacgatt ccacggtaca cgcagtgcac cgtggaaaac taatgcggag
     4441 gtggggatcg acgcgcgaag gcacgcgcac gacggctgga ccgatcaggg ggctgatcga
     4501 gaaagcttac tcgtcgttct tggtctggag gacctttttg ccgcgataga caccgttcgg
     4561 actgatgtga tgacggcgat gcacttcacc ggtcgtcggc tcgatcgccg tcggcgggtt
     4621 cgtcagaaag tcgtgcgcgc gatgcgttcc gcgtttcgac gacgacttct tgttctgttg
     4681 gacagccatg gctaacacca cgaaagaaaa caggaaattg tacccgacgc ggcgcggcag
     4741 tggaaaactt ccgcctagct cgcctttgaa cccggcttca ggtcggcaag caccgcgaac
     4801 gggtgctcac gcccggtagg ttctctgctc ggttcctctg ctgcccccag ctcagccgcg
     4861 ggacggcacg ccgcgtcatc ccatgggtga cgaggtatca gtggcagtga caaaatcgcc
     4921 tcgtcctcga tccaggtcga gacatccatc ctgcgcgcgc cgacgatggc gtcgacctca
     4981 tcgtcctcga tcggcaaggc attcagttct tcttcgccgt cgacgaagcg gaattgcgct
     5041 tcgcgctcga gaaggaaggt gtagggctcg ttgcaacgct gacactcgag caccaggtcg
     5101 ccctgcaaat gcatgtgggc ccctgggcgc cctcgctcgt ccagttggcc ctgaatccga
     5161 taacgaagca ctccttccgg ccgggccagt gccgctgcga gccgcggcag cgatgccagc
     5221 gaaaggctgc cttcgattgt ggccttgtca cgggccaact cgaacacatc gatatacgaa
     5281 tgggaacgcg aggtcatgga gagaattttg gagtcgagat ccccgcacgc gcggctacac
     5341 tgcgcgcgcc atggactcgc cgcgcatcgt actcgcctcg acgtcgcgct atcgccgcga
     5401 gttgctcggt cgccttcggc tgacattcga cgtgagcgcg ccgcaggtcg atgagtcccc
     5461 gctcgcccgc gaaacgcccg gcgcaacggc acttcgactg gccgtcgcaa aggcgcgcga
     5521 ggtgcatgcg cgccacccgg gagcgatcgt catcggctcc gatcaggtcg ccgagctgga
     5581 cggcagcgcg ctcggcaagc cgaaccagca cgaagccgca ctggcgcaac tcgaactgat
     5641 gcaggggcgc gaggtcgtgt ttcatacggc cgttgcagtc gcgggagccg agctcgaggc
     5701 attgcaggtc gacaatattc caacatacgt gacgatgcgg tcgttgccac gcgcggcact
     5761 cgagagctac ctccgcgcgg atgaaccgta cgactgcgcc ggtgcggcga agatcgaatc
     5821 cctcggcatc gcgttggtgg atcgcgtccg gtcggatgac ccgaccgcgc tgatcgggct
     5881 tccgctgatt cgactcaccg ccatgctggc ggccgtgggc attgcggtgc tgccggtgcg
     5941 ctgagtcgtg gcgactctct acctgctgcc ctgcgcgatc gccgaaggct cggagctgtc
     6001 ggtgttgccg gcccaggtca tcgcgatcgc ccaacgcctg acctattttc tcgcagaaaa
     6061 cgcaaagtcg gcccgtgcct tcctaaaggc gatcggccat ccacagccct tgcgtgagtt
     6121 gcagatcatc gagatcgggc atgcgccccc cgcggctgac atccctgcct ggctcgaacc
     6181 gttgattgcc ggacttgacg gcgcgctggt ttccgaagcc ggttgcccgg ccgtggccga
     6241 tccaggcgcg gaaatcgttg ctgccgctca tcaacgcgga ctgcgagtcc agccgctggt
     6301 aggtccgtcg tcgctgctgc ttgccctgat ggcgtcggga ctgaatggcc agcagttccg
     6361 ttttcatggg tatctgccgg ttcccgccgg cgaccgaacc cggcgtctgg tcgcgcttga
     6421 gcgagactcg cgcgccgggg ggacgcaact cttcattgag acgccctatc gcagccccgc
     6481 gatgttcgac gccatcatga cttcatgcgc gccggccacg cggctgtcga tcgcgattga
     6541 tctgaccggt ccgtcagagt ttgtgcagac ccgcacgatc gcggagtgga agcgcaccca
     6601 tcgaccgccg gtggagcgac gcccgaccgt gttcctgctt ctcgcctaaa ggctgtcgtc
     6661 cagcggtcga gtgtgatggt cgggttccca gcagaccgtc gttgcactct cgacaactgt
     6721 tagaggtcgc aggccaccgt gcccggcaca ggcgccgccg cgacatgcgc cggtggccgg
     6781 gtcgtacagg gcgccgtggg tcgagcacat caggaactca gcatcgggct cgaagaattc
     6841 gcccggctgc cagtcgagtt ccatcgccac atgcgcgcag cgattgaggt acgccaccgc
     6901 gatcccccga tggcgtacgg caaaggccgg tgcccgttcg ccacggaaca tgacttcgaa
     6961 gcgcacgcca cgcgcgccat ccttcagtgc gtccggggag cagatgggaa tcagcgcgtc
     7021 gccagcagcc atcggtggag ctccttgacc gacgcggcga gtcccagcgg ttcatatgac
     7081 gccagttgtt cgcgcgggtg ggcgccgtag gtgaccccta tcgacgcggt gcctgccgac
     7141 cgcgccatcg cgagatcgtg cgtcgtgtcg ccgatcatca cggtccgggc tcgaggcacc
     7201 gcgagttcct ccgtgagttc gagcagcatc gccggatgcg gctttggatt cgtctcgtca
     7261 gcgcagcgtg acgatgcaaa gaaca
//
LOCUS       DATIRT010000469         3181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1753389, whole genome shotgun sequence.
ACCESSION   DATIRT010000469 DATIRT010000000
VERSION     DATIRT010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1753389"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /gene="rpsH"
                     /locus_tag="VLN25_10650"
     CDS             <1..282
                     /gene="rpsH"
                     /locus_tag="VLN25_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HSQ11054.1"
                     /translation="VLKDEGYIEGFQVNANDGKPELRIGLKYYAGRPVIESLERVSRP
                     GLRVYKGSDDIPAVMNGLGVAIVSTSRGVMTDRRARSQGVGGEVLCYVA"
     gene            300..833
                     /gene="rplF"
                     /locus_tag="VLN25_10655"
     CDS             300..833
                     /gene="rplF"
                     /locus_tag="VLN25_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HSQ11055.1"
                     /translation="MSRVARIPVTVAKGVDVQVTEHTITVKGPLGTLHQALSGRVKVE
                     LANGEVSFAAADESREANAMSGTMRALVAAMVQGVSKGFEKKLSLVGVGYRAQAQGDK
                     LNLSLGFSHPVVHQMPTGVKCETPTQTEIVIKGADKQKVGQVAAEVRGYKPPEPYKGK
                     GVRYANEVVVIKETKKK"
     gene            851..1219
                     /gene="rplR"
                     /locus_tag="VLN25_10660"
     CDS             851..1219
                     /gene="rplR"
                     /locus_tag="VLN25_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HSQ11056.1"
                     /translation="MIEKKETRLRRARATRARIALQKTERLTVSRTNLHIYASVVSAD
                     GGKVLVSASTVEPEVKTQLATAKGKGSNVDAAKVVGARIAQKAKAAGIESVAFDRAGY
                     RFHGRVKALADAAREAGLKF"
     gene            1231..1752
                     /gene="rpsE"
                     /locus_tag="VLN25_10665"
     CDS             1231..1752
                     /gene="rpsE"
                     /locus_tag="VLN25_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HSQ11057.1"
                     /translation="MARVQPKGVVSEDRDDGIKEKMIAINRVTKVVKGGRILGFAALT
                     VVGDGDGRIGMGKGKAREVPVSVQKAMEKARRAMAKMPLKNGTVHHFVEGKHGASRVM
                     LSPAADGTGVIAGGPMRAVFDVMGIRNIVAKSHGSTNPYNLVRATLDALSKLRTPAEI
                     AAKRGKSVEEVFG"
     gene            1767..1946
                     /gene="rpmD"
                     /locus_tag="VLN25_10670"
     CDS             1767..1946
                     /gene="rpmD"
                     /locus_tag="VLN25_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HSQ11058.1"
                     /translation="MKKMLKVTLVKSPISAKVSHQATVRGLGLKKMRQTRELEDTPAV
                     RGMINKVAYLVQVGE"
     gene            1962..2393
                     /gene="rplO"
                     /locus_tag="VLN25_10675"
                     /pseudo
     CDS             1962..2393
                     /gene="rplO"
                     /locus_tag="VLN25_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197941.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
     gene            2604..>3181
                     /gene="secY"
                     /locus_tag="VLN25_10680"
     CDS             2604..>3181
                     /gene="secY"
                     /locus_tag="VLN25_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431626.1"
                     /note="forms heterotrimeric complex in the membrane; in
                     bacteria the complex consists of SecY which forms the
                     channel pore and SecE and SecG; the SecG subunit is not
                     essential; in bacteria translocation is driven via the
                     SecA ATPase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HSQ11059.1"
                     /translation="MATSASSTIGSTGKYGDLKRRLFFLLLALVVYRVGTHIPVPGID
                     PDQLRQLFNSQQGGILGLFNMFSGGALSRFSVFALGIMPYISASIIMQMLTYVLPSLE
                     ALRKEGESGRRKITQYTRYGTLGLAIFQAIGISVALESQQGLVIDPGLMFRFICVVSL
                     VTGTMFLMWLGEQITERGLGNGISILIFAGIVA"
BASE COUNT          652 a          914 c         1101 g          514 t
ORIGIN      
        1 gtcctgaagg atgagggcta catcgagggc ttccaggtga atgccaacga tggcaagcct
       61 gaattgcgca tcggtctgaa gtactacgcc ggccgcccgg tcatcgagag ccttgagcgc
      121 gtatcgcgtc cgggtctgcg cgtttacaag ggcagcgacg acattcccgc cgtgatgaac
      181 ggactgggcg tggcaattgt ctcgacctcg cgcggggtga tgaccgaccg cagggcccgc
      241 agccagggcg tcggcggcga agtgctctgc tacgtcgcgt aacggagcgg agggattcca
      301 tgtctcgagt agcacgaatt ccggtgaccg tcgccaaggg cgtcgacgtt caggtcacgg
      361 aacacaccat tacggtcaag gggccgctgg gcacgcttca tcaggcgctg tcgggccgcg
      421 tcaaggtcga actggccaat ggcgaagttt cgttcgccgc ggccgacgag tcgcgcgaag
      481 ccaacgccat gagcggcacc atgcgcgcgc tggtagcggc catggtgcag ggtgtttcga
      541 agggcttcga aaagaagctg tcgctggtcg gcgtcggtta ccgcgcccag gcgcagggcg
      601 acaagctgaa cctgtcgctg ggtttttcgc accctgtcgt tcatcagatg cccaccggtg
      661 tgaagtgcga aacgccgacg cagaccgaga tcgtcatcaa gggcgctgac aaacagaagg
      721 tcgggcaggt cgcggcggaa gtccgtggct acaagccgcc ggagccctac aagggcaagg
      781 gcgtgcgcta tgccaatgag gtcgtggtca tcaaagagac caagaagaag taaggcggcg
      841 agaggtcaac atgatcgaga aaaaggaaac ccgtctgcgt cgtgcccgcg cgacccgcgc
      901 ccgcattgcg ctgcagaaga ccgagcgcct gacggtcagc cgcaccaacc tgcacatcta
      961 cgccagtgtc gtatcggccg atggtggcaa ggtgctggtg tcggcgtcga ccgtcgaacc
     1021 ggaagtcaaa acgcaactgg cgaccgccaa gggcaagggc agcaacgtcg atgcagccaa
     1081 ggtcgtcggt gcccggatcg cacagaaggc gaaggcagcc ggcatcgaga gcgtggcttt
     1141 cgaccgcgcg ggttaccgat tccacggccg ggtcaaggcg ctggcggatg ccgcgcgtga
     1201 agccggactg aagttctaag aggaagcccc atggcacgag tgcaaccgaa aggtgtcgtc
     1261 tccgaggatc gtgacgacgg catcaaagaa aagatgatcg cgatcaaccg cgtcaccaag
     1321 gtggtgaagg ggggtcggat tcttggattt gctgcgctga ccgtggtcgg cgacggcgac
     1381 ggccgcatcg gcatgggcaa gggcaaggcg cgcgaggtgc cagtctcggt ccagaaggcg
     1441 atggagaagg cgcgccgcgc catggcgaag atgccactga agaacggaac ggtgcatcac
     1501 tttgtcgaag gcaagcacgg cgcgtcgagg gtaatgctgt cgccggcggc agacggtacc
     1561 ggcgtcatcg ctggcggccc gatgcgtgca gtttttgacg tcatgggcat tcgcaacatc
     1621 gtggcgaagt cccatggctc gactaatccg tacaacctgg ttcgggccac gctggatgcg
     1681 ctcagcaagc tgcgcacccc ggccgagatc gcggccaagc gcggcaagtc ggtcgaggaa
     1741 gtgttcggct gagccggaga gagacgatga agaagatgct caaggtcacg ctggtcaaga
     1801 gcccgatctc cgccaaggtg tcacaccagg cgaccgtgcg cggactcggc ctgaagaaga
     1861 tgcgccagac acgtgaactg gaagacacgc cagccgtgcg cggcatgatc aacaaggttg
     1921 cctacctggt ccaggtcggc gagtaatcac aggggtcgac gatgcgcctc aacaacctga
     1981 aaccggcggc cggcagcaag agcgcccgtc accgtgtcgg ccgcggcatc ggctccggct
     2041 ggggcaagac cgcgggccgc gggcacaagg gccagaagtc tcgtgccggc ggctttcaca
     2101 aggtcggttt cgagggcgga cagatgccgc tgtaccggcg cctgccaaag cgcggcttca
     2161 cgtcgcttac gcgccggtat gtggaagtcg tgcggctggc ggacctgcaa cgactgggcg
     2221 tcgacgagat cgatctcgcg gtgctcaagc aagcagggat tgtgtcggcg ctcgcgctcg
     2281 atgcgcgcgt cgtgaagacc ggcgaactga gccgcaaggt cgtggtgcgt ggcatcggtg
     2341 tgaccgccgg tgcgaaagcg gcaatcgaag cggcgggcgg ttctgtcgct gaatcggcgg
     2401 tgacgccgga cacggccaag aaggcagcgg caaggaaagc ggcggcagat aaggcggctg
     2461 cgatcaaggc ggcggtgaac aagccagccg aggccaaggc caaggccgag aagccggtca
     2521 agtccgacaa gcaggccaag gccgagaagc aagcgaagta atcagcaaga cacgcaaagc
     2581 gacaagcgaa acgcaggaac acagtggcga ccagcgcatc ttcgacgata ggcagcaccg
     2641 ggaagtacgg cgacctgaaa cggcgtctgt tctttctgtt gctggcgctc gtcgtctatc
     2701 gggtgggaac gcacatcccg gtgccgggca tcgatccaga ccagctgcgt cagctgttca
     2761 actcgcagca gggcggcatc ctcggcctgt tcaacatgtt ctcgggtgga gcgctgtcgc
     2821 gcttttcggt gtttgcactc gggatcatgc cgtacatctc ggcgtcgatc atcatgcaga
     2881 tgctgaccta tgtgctgcct tcgctcgaag cgctgcgtaa ggaaggcgaa tcgggccgcc
     2941 gaaagatcac gcagtacacg cgttacggca cgctcggttt ggccatcttc caggcgatcg
     3001 gcatttcggt tgcgctggaa tcgcagcagg gtctggtgat cgatccgggc ctgatgttcc
     3061 gcttcatctg cgtcgtgtcg ctggtcaccg ggacgatgtt tctgatgtgg ctgggcgagc
     3121 agatcaccga acgcgggctg ggcaatggca tttcgatcct gatcttcgcc ggcatcgtgg
     3181 c
//
LOCUS       DATIRT010000470         3716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1359800, whole genome shotgun sequence.
ACCESSION   DATIRT010000470 DATIRT010000000
VERSION     DATIRT010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359800"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2043)
                     /gene="acnA"
                     /locus_tag="VLN25_10685"
     CDS             complement(<1..2043)
                     /gene="acnA"
                     /locus_tag="VLN25_10685"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HSQ11060.1"
                     /translation="MAHNLFNSLQDFTIGAKKGRFYNLSALEKAGVGKISRLPVSIRI
                     VLESVLRNCDGIKVTEEHVRQLANWKPNAERTDEIPFIVARVVLQDFTGVPLLADLAA
                     MRNVAAAMGKNPKTIEPLVLVDLVVDHSVMIDHYGGKDSLDLNMKLEFQRNRERYEFM
                     KWGMQAFDTFGVVPPGFGIVHQVNLEYLARGVHKKDGVVYPDTLVGTDSHTTMINGIG
                     VVGWGVGGIEAEAGMLGQPVYFLTPDVVGVELTGRLREGVTATDLVLTITELLRKSKV
                     VGKFVEFFGEGTASLALPDRATIGNMAPEYGATMGFFPVDEKTIAYFEGTGRTPDEIA
                     AFEAYFRAQGLFGVPKSGSIDYSQVVKLDLSTVAPSLAGPKRPQDRIEIGNVKKQFTS
                     LFSKPVSENGFNQPADRLKQRFPVSEGHGRMEGKAPDTPAGAARDLAEMSANRPMVKP
                     SREHEVHEVTLADGDVLIAAITSCTNTSNPSVLLAAGLLAKKAVERGLTVRKHIKTSL
                     APGSRIVTEYLSKTGLLPYLEQLGFSVAAYGCTTCIGNAGDLAPEFNETITKNDLVCA
                     AVLSGNRNFEARIHPNLKANFLASPPLVVAYAIAGTMLKDLMTEPLGKGKDGKDVWIG
                     DIWPTSDEVHALMKSALDPVAFEANYSKVKSDPGDLWKKVGTVTGQVYTWPTSTYIA"
     gene            complement(2081..3532)
                     /locus_tag="VLN25_10690"
     CDS             complement(2081..3532)
                     /locus_tag="VLN25_10690"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HSQ11061.1"
                     /translation="MGSHISNVRPKPDKVLVDIADYVIKYKVKSKEAIDTARYCLMDT
                     LGCGFEALEYPACTKMLGPVVHGTVVPNGAKVPGTQFQMDPVKAAFDIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVAAGKSPLTVSDVLAAMVKAHEIQGCIAL
                     ENSFNKVGLDHVVLVKVASTAVFAQMLGLTRDEIINAVSLAWVDGQSLRTYRHAPNTG
                     SRKSWAAGDATSRAVRLALIAKSGEMGYPSVLSAKGWGFYDVLFKGNEFKFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTIHAELKKLGKTEKDIRKITIRTHEACIRIIDK
                     KGPLSNPADRDHCIQYMVAVPIIFGRLTAGDYEDSIAADPRIDVLREKIECVEDKSFT
                     KDYHDPAKRSIANALTVEFTDGKKLKEAVVEYPIGHKRRRKEGMPVLVAKFQTNLARV
                     FPSKQAATILDLCLDAKRLDATPVNEFVDLMVI"
BASE COUNT          605 a         1170 c         1180 g          761 t
ORIGIN      
        1 cgcaatgtag gtcgacgtcg gccacgtgta gacctgaccg gtaacagtgc cgactttctt
       61 ccacagatcg cccggatccg acttgacctt gctgtagttg gcttcgaagg cgaccgggtc
      121 gagcgccgat ttcatcagcg cgtgcacttc atccgacgtc ggccagatgt cgccgatcca
      181 gacgtccttg ccgtccttgc ccttgccgag cggctcggtc atcaggtcct tgagcatggt
      241 gccggcgatg gcgtaggcga ccaccagcgg tggcgacgcg aggaagttgg ccttgaggtt
      301 cgggtggatg cgggcttcga agttgcggtt gcccgacagt accgctgcgc acacgagatc
      361 attcttcgtg atggtttcat tgaactccgg cgccaggtcg cctgcattgc cgatgcaggt
      421 ggtgcagcca taggccgcca ccgagaatcc gagctgctcc agatagggca gcaggcctgt
      481 cttcgacagg tactcggtca cgatccgtga gccgggcgcg agcgaggtct tgatgtgctt
      541 cctgaccgtg aggccgcgct ccaccgcctt cttggccagc aggccagccg caagcagcac
      601 gctggggttg gacgtattcg tgcacgaggt gatcgcggcg atcaagacat cgccgtcggc
      661 gagcgtgact tcatgcactt catgttcgcg gctcggcttg accatcggac ggttcgcact
      721 catttccgcc aggtcgcgcg ccgcgccggc cggcgtgtcc ggcgccttgc cttccatgcg
      781 tccgtggccc tccgaaaccg ggaagcgctg cttgagccga tcggccggct ggttgaaacc
      841 gttttcgctc acgggcttgg aaaagagcga cgtgaactgc ttcttgacat taccgatctc
      901 gatccggtcc tgcggccgct ttggtccggc caacgacggt gcgaccgtgg acaggtcgag
      961 cttgacgacc tgtgaatagt cgatcgaacc ggacttcggc acgccgaaca gtccctgcgc
     1021 tcggaagtag gcctcgaagg ccgcgatctc gtccggcgtg cgtcccgtgc cttcgaagta
     1081 ggcgatcgtc ttctcgtcga ccgggaagaa gcccatcgtc gcgccatatt ccggcgccat
     1141 gttgccaatc gtggcgcggt cgggcagcgc gagtgaggcc gtgccttcgc cgaagaattc
     1201 gacgaacttg cccacgacct tggacttgcg cagcagctcg gtgatcgtca gcacgaggtc
     1261 ggttgcggtc acgccttcac gcaggcgccc ggtcaattcg actccgacga catcgggcgt
     1321 caggaagtac accggctggc cgagcatgcc ggcttcggcc tcgataccgc cgacgcccca
     1381 gccgaccacg ccgatcccgt tgatcatcgt cgtgtggcta tcagtgccga ccagcgtgtc
     1441 ggggtagacc acgccatcct tcttgtgcac accgcgcgcc aggtactcga ggttgacctg
     1501 gtggacgatg ccgaagcccg gcggcacgac accgaatgta tcgaacgcct gcatgcccca
     1561 tttcatgaac tcgtagcgct cgcggttgcg ctggaactcg agcttcatgt tgagatcgag
     1621 cgagtccttg ccgccgtagt ggtcgatcat caccgagtga tcgacgacca ggtcgaccag
     1681 cacgagcggt tcgatcgtct tcgggttctt gcccatcgcg gcagccacgt tgcgcatcgc
     1741 ggccaggtcc gccagcagcg gcacgcccgt gaagtcctgc agcaccacgc gcgcgacgat
     1801 gaacgggatc tcgtcggtgc gctcggcatt cggcttccag ttcgccagct ggcgaacgtg
     1861 ctcttccgtg accttgatgc cgtcgcagtt gcgcaggacg gattcaagca cgatgcggat
     1921 cgacaccggc aggcgcgaga tcttgccgac gccggctttc tccagcgccg acaggttgta
     1981 gaacctgcct ttcttcgcgc cgatcgtgaa gtcttgcagg gaattgaaca ggttgtgagc
     2041 catgggaggg cctcggagtc ggtgggcttg cgatgacggg ctagataacc atcaggtcga
     2101 cgaattcatt gacaggcgtg gcgtcgagcc gtttcgcgtc caggcacagg tcaaggatcg
     2161 tcgcggcctg cttcgacggg aaaacccgtg cgaggttggt ctggaacttc gcgaccagca
     2221 ctggcatgcc ttccttgcgg cgccgcttgt gcccgatcgg gtactccacg accgcttcct
     2281 tcagcttctt gccgtcggtg aactccacgg tcagcgcgtt ggcgatcgag cgcttggccg
     2341 ggtcgtggta gtccttcgtg aaggacttgt cttcgacaca ctcgatcttc tcgcgcagca
     2401 cgtcgatgcg cggatcggcg gcgatcgagt cttcgtagtc gcccgccgtg aggcgcccga
     2461 agatgatcgg caccgccacc atgtactgga tgcagtggtc gcggtcggcc gggttgctga
     2521 ggggcccctt cttgtcgatg atgcggatgc acgcctcgtg cgtgcggatc gtgatcttcc
     2581 tgatgtcctt ctcggtcttt ccgagtttct tcagttccgc atggatcgtc atcgcggctt
     2641 caacggcggt ttgcgagtgg aactcggccg ggaagctgat cttgaagagt acgttttcca
     2701 tcacatagct gccatagggt cgctggaatt tgaactcgtt gcccttgaac agcacgtcgt
     2761 agaaacccca gcccttggcg gacagcaccg acggatagcc catctcgccg gacttcgcga
     2821 tcagcgccag gcgcaccgct cggctcgttg cgtcgcccgc tgcccagctc ttgcgcgagc
     2881 cagtgttggg cgcatgacgg taggtccgca gcgactgacc gtcgacccag gcaagtgaca
     2941 ccgcgttgat gatctcgtcg cgcgtgagcc ccagcatctg cgcgaagacc gccgtggacg
     3001 cgaccttgac cagcacgacg tggtcgaggc cgaccttgtt gaacgagttc tcgagcgcga
     3061 tgcagccctg gatctcgtgg gccttgacca tcgctgccag cacgtcggat accgttagcg
     3121 gtgactttcc agcggcgacc gcgttgcggg aaagccagtc ggcggtggcc aggatgccgc
     3181 ccaggttgtc cgagggatgg ccccattcgg ccgcgagcca cgtgtcgttg aagtcaagcc
     3241 agcggatcat cgcgccgatg tcgaacgccg ctttcaccgg atccatctgg aactgcgtgc
     3301 ccggcacctt cgcgccgttc ggcaccacgg tgccatggac gaccggaccg agcatcttgg
     3361 tgcaggccgg atactccagc gcctcgaagc cacagccgag cgtgtccatc agacagtagc
     3421 gcgcggtgtc gattgcttcc ttgctcttga ccttgtactt gatgacgtag tcggcgatgt
     3481 cgaccagcac cttgtcaggc ttcgggcgga cgttgctgat gtggcttccc atgaggggat
     3541 gatcctttct gctccagtgt tcgatggttc gtgtcgatgt tcgatgggtc tatttgtccg
     3601 gcgcgggtcg cttgccgccg gcctggaaga cggcgcagtt ctgtgccagc gcggaaccgc
     3661 tggagatcgc gagccggctg atggcaccct tgctccacca ctcccaggcg ccgtcc
//
LOCUS       DATIRT010000471         2318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_277971, whole genome shotgun sequence.
ACCESSION   DATIRT010000471 DATIRT010000000
VERSION     DATIRT010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277971"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VLN25_10695"
     CDS             complement(<1..657)
                     /locus_tag="VLN25_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11062.1"
                     /translation="MTRLLTPSPDDRFGIGAKLYCLWAATAIGVSLVLAGPTAETITR
                     LLVLAFIAGQYVFRATLVNALPRLPPQARFITMGVLLAAVVEGFHMISKPVYSSLLVG
                     PDTLPAEALWFYGIDLLVTTPAYIVIFAVIWFLINRFHYGLWSYIVVMGLGQALGDGG
                     VFFFAAAPQMVVFLPYPMTNYHACNVLPFVATRDALPAARVAGRRAWLAVPVLIAVYL
                     V"
     gene            complement(710..1057)
                     /locus_tag="VLN25_10700"
     CDS             complement(710..1057)
                     /locus_tag="VLN25_10700"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HSQ11063.1"
                     /translation="MNITRHDQSAILSQAVEHGETVYLAGIVAKDLSKDVKGQTREVL
                     GEIDRLLAKCGSSKSRVLQCHIWVTDIRNRAPMNEVWTEWVDMNNLPARACVEAKLAD
                     PDALVEIMVIAAR"
     gene            complement(1171..1246)
                     /locus_tag="VLN25_10705"
     tRNA            complement(1171..1246)
                     /locus_tag="VLN25_10705"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1211..1213),aa:Phe,seq:gaa)
     gene            1441..>2318
                     /locus_tag="VLN25_10710"
     CDS             1441..>2318
                     /locus_tag="VLN25_10710"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSQ11064.1"
                     /translation="MSRSVLRCLAAAVLSVALGASAHAADLTIGLSTPVTSLDPHFHN
                     LTPNNSLNRHIFETLIKQDESQRMQPGLAESWKALNDLEWEVKLRKGIKFHNGQEFTA
                     DDVIATFKRVPNVPNSPASFAFFVRPIVEISAPDKHTLRMKTDKPHPLLPNDLGAIMI
                     LPKAVAETAKTEDFNSGKAMIGTGAYRLTEYVAGDRVVLKRNDAYWGTKPTWDNVRFK
                     MIPSAPARVAALLAGDVQMIEGVPTADIAKLSKDGRVDLSSAVSNRIIYLHMDSAREK
                     KSPFVTDPAGKPLDANP"
BASE COUNT          483 a          736 c          702 g          397 t
ORIGIN      
        1 gacgaggtag acagcgatca ataccggtac cgccaaccac gcgcgtctgc ccgccacgcg
       61 cgctgccggc agcgcgtcgc gcgtcgccac gaatggcagg acgttgcacg cgtgatagtt
      121 cgtcattggg taaggcaaaa ataccaccat ctgcggcgct gctgcgaaga agaaaacgcc
      181 gccgtcaccg agggcctgcc ccagacccat caccacgatg tacgaccaga ggccgtagtg
      241 gaatcggttg atcaagaacc agatgacggc aaagatcact atgtatgcgg gcgtggtcac
      301 gagcaggtcg atcccataga accacagcgc ctcggctggc agcgtgtcgg gcccgaccag
      361 cagcgatgaa tacaccggct tcgagatcat atgaaagccc tcgacgacgg cggccagcag
      421 cacgcccatc gtgatgaacc gtgcttgcgg cgggagtctt ggcagcgcgt tcaccaaggt
      481 tgcgcgaaac acgtactggc cagcgatgaa cgcgagcacc agcagtcgcg tgatcgtctc
      541 tgcggtcggt ccggccagta ccagcgacac gccgatggca gttgcggccc acaggcaata
      601 gagcttcgcg ccgattccga atcgatcgtc gggcgaaggc gtcaggagtc tagtcaatcc
      661 accacccaag tacgacgact ttcttaccga tcgcaatggg ccgatagcgc tacctggcgg
      721 cgatcaccat gatctccacc agagcgtccg gatcggcaag cttggcctcg acgcacgcgc
      781 gtgccgggag gttgttcatg tcgacccact cggtccacac ttcattcatt ggcgcgcggt
      841 tgcggatgtc ggtgacccaa atgtggcact gcaggaccct ggatttgctg gagccgcact
      901 tcgcgagaag tcgatcgatc tcaccgagga cctcccgagt ctgacccttg acgtctttgc
      961 tcaagtcttt ggcgacaatc cccgccaggt agacggtttc accgtgctct actgcttgcg
     1021 aaagtatggc gctctggtcg tgtctcgtga tattcacaaa tcggctcctg cgggtaagga
     1081 catgtcggcg gaagagtgag ttggagccct ttggccgatt caatgagtcg gctgggcaaa
     1141 acgcacagcg tggttgcgca gaatgaaaag tggtggccaa ggacggaatt gaaccgccga
     1201 cacaaggatt ttcaatcctc tgctctacca actgagctac ttggcctggg atctgcagaa
     1261 cggcgaacgt tgctaccaat gccgtcgcga agggcgcgaa gtatagcgga gccgtccacg
     1321 tgccggagcc gctgcgcgcg gtccgaacgt cacagcagcc acctcgagtc gcgcgcggct
     1381 tcgccgccgc cgccaacgcg gctaagctac ccgggttcac cctgtctgga gagctgaaaa
     1441 atgtcccgct cggtcctgcg ttgccttgct gcggctgtct tgtccgtggc cctcggcgcg
     1501 tccgcgcacg ccgccgatct caccatcgga ttgtcgaccc cggtcacgtc cctcgatccg
     1561 catttccaca acctgacgcc gaacaacagc ctcaaccggc acatcttcga gacgctgatc
     1621 aagcaggacg agagccagcg catgcagccg gggctggcgg agtcgtggaa ggcgctgaac
     1681 gacctcgagt gggaggtcaa gctgcgcaag ggcatcaagt tccataacgg ccaggaattc
     1741 accgccgacg acgtgattgc gacgttcaag cgagtgccca acgtgccgaa cagcccggcc
     1801 tcgttcgcct tcttcgtgcg gcccatcgtc gaaatcagtg cgcccgacaa acacacactg
     1861 cggatgaaga ccgataaacc gcacccgctc cttccgaacg acctcggcgc gatcatgatc
     1921 ctgccaaagg ccgtggccga aacggcgaag accgaggact tcaactccgg caaggccatg
     1981 atcgggaccg gcgcctaccg gctgaccgag tatgtcgcgg gcgaccgcgt cgtgctgaag
     2041 cgcaacgatg cgtattgggg cacgaagccg acgtgggaca acgtccgctt caagatgatt
     2101 ccgagcgcgc cggcacgcgt agccgcgctg ctggccggcg acgtgcaaat gatcgagggc
     2161 gtgccgactg ccgacattgc caagctgtca aaggatgggc gcgtcgacct ctcgtccgcg
     2221 gtgtcgaacc ggatcatcta cctgcacatg gattcggcgc gcgagaagaa gtcgcccttc
     2281 gtcaccgatc ctgcgggcaa gccgctggac gcgaaccc
//
LOCUS       DATIRT010000472         7690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1065537, whole genome shotgun sequence.
ACCESSION   DATIRT010000472 DATIRT010000000
VERSION     DATIRT010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7690
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065537"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VLN25_10715"
     CDS             complement(<1..465)
                     /locus_tag="VLN25_10715"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HSQ11065.1"
                     /translation="MNQPIEPLDCLPAAARPARRRPTRQVELRWGARVVKMGADAPVL
                     VQSMTNTDTADAIATAVQVKELAQAGSEAVRITVNSPEAAAEVAAIREQLDRMGIDVP
                     LIGDFHYNGHKLLTQHPGCAQALSKYRINPGNVGKGVRREDNFAQMIEVACRN"
     gene            complement(473..1357)
                     /locus_tag="VLN25_10720"
     CDS             complement(473..1357)
                     /locus_tag="VLN25_10720"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HSQ11066.1"
                     /translation="MSDDADTAAAGVDAQASTAAEAPSRPTSFGARLKWEREQAGLTV
                     SDVAARLRLHPNQIRAIEQESLAALPEPAYVRGFVRSYARIVNADPVPLLDDLSTKMV
                     PAERSVVDGMAQPRDYSPVRAAAREQASRRVVVLGAVLVLIVLGALGWYATRQQAETR
                     ASVSPTKLAAAPPVATTPPAVVPAVASALGADAAPATAGSAGSAGESAGVMEPVAPPP
                     LLKLRFAGPSWAEVKDAEGKVLHSQHNSPGAEFVIEGTPPFYVVIGDATKTTVEVRGE
                     VFDMVPHTKQYVARFTIN"
     gene            complement(1350..2165)
                     /gene="pilW"
                     /locus_tag="VLN25_10725"
     CDS             complement(1350..2165)
                     /gene="pilW"
                     /locus_tag="VLN25_10725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HSQ11067.1"
                     /translation="MRGGLIAQLIVVACAGILAACTTTTVTTSSGEVVSTTQSGAAGA
                     SPAVTKSRARARVELAAGYFRNGQLAVALEEAKRAVQIDPTFADAYGLLGLIYMELED
                     RRAAEENFQRALQLDPSNADLNNNYGWYLCQTGREGESIAYFQRSLRDPLYATPARAN
                     FNAGHCLLRVKDYPGAEPYLRRSFELDATSVTTKYELARLYLATGQTDRASFYYGLLV
                     KSAEASPETVWLGLKVARAQGDLRTETRLASELRTRFPGSKEAGMLARGAFDE"
     gene            complement(2188..3306)
                     /gene="rlmN"
                     /locus_tag="VLN25_10730"
                     /pseudo
     CDS             complement(2188..3306)
                     /gene="rlmN"
                     /locus_tag="VLN25_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008811550.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
     gene            complement(3315..3740)
                     /gene="ndk"
                     /locus_tag="VLN25_10735"
     CDS             complement(3315..3740)
                     /gene="ndk"
                     /locus_tag="VLN25_10735"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HSQ11068.1"
                     /translation="MAVERTLSIIKPDAVAKNVIGKIYSRFETNGLKVVAARMARLSR
                     HEAEGFYAVHRERPFFKDLVDFMVSGPVMIQVLEGESAIAKNRELMGATDPKKAAAGT
                     IRADFAESIDANAVHGSDGEDTARIEIAYFFPQMAVYSR"
     gene            complement(3936..5303)
                     /gene="rlmD"
                     /locus_tag="VLN25_10740"
     CDS             complement(3936..5303)
                     /gene="rlmD"
                     /locus_tag="VLN25_10740"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016475389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HSQ11069.1"
                     /translation="MKSGEIFQVEIESLDQEGRGVGHREGKAVFVEGALPGEQATYER
                     RRNKASYEVGRTVAIERVSSLRATPRCPHFGLGAGSCGGCSMQHLDARAQVAIKQRVL
                     EDSLWHIGRLRAERMLRPIAGPTWNYRYRARLTVRDVAKKGEVLVGFHERASSFVADM
                     TRCEVLPVRVSDLLVPLRRLVESLSLRDRLPQIELAAADRTDGLQVVLVLRVLQEPTT
                     DDRTRLREFADRHSVEFWLQPGGPHTAVPLTVEQPSELRLGLPEFGVTLSFSPTDFTQ
                     VNHSVNEALVSRAVRLLAPTPQESVVDFFCGLGNFTLPLATRARHVVGYEGSSNLVER
                     ARAAADANALGDCTSFEARNLFQWSATDWEAAVERRGGIDKVLIDPPREGALAIVQVL
                     AVTERPPARVVYVSCNPATLARDCAVLVHEGDWILRAAGIVNMFPHTSHVESIAMLEP
                     GLARP"
     gene            complement(5305..6084)
                     /locus_tag="VLN25_10745"
     CDS             complement(5305..6084)
                     /locus_tag="VLN25_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HSQ11070.1"
                     /translation="MTPILVFDIETVPDIAGARKLRAQFSGLSDPDVAQRLFDERREK
                     TGSDFLPLHLHRVVAISCVFRDDSGFRVRSIGHPDDDEGKLIQAFFATVDRYTPQLVS
                     WNGGGFDLPVLHYRGLIHGVHATRYWELGDDDRDFRYNNYIGRYHTRHIDLMDLLAMY
                     QPRASAPLDDLAKLCGFAGKLGMDGSRVWEAYQAGGIEQIRAYCETDVVNTYLLFARF
                     QRMRGNLDDGGYAEEEGRVHAALTALGQPHWKEYLAAWGRT"
     gene            complement(6113..7105)
                     /gene="rpoS"
                     /locus_tag="VLN25_10750"
     CDS             complement(6113..7105)
                     /gene="rpoS"
                     /locus_tag="VLN25_10750"
                     /inference="COORDINATES: protein motif:HMM:NF004207.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HSQ11071.1"
                     /translation="MAGIREIPDTPACEPDDANASERSAFERDAPGESDEFARALNGM
                     EAEASDDDYEPDNVQRYLADIGTHPLMSAEEERATADRARAGDFAARQQMIERNLRLV
                     VSIVKRYAHRGLPLLDLIEEGNLGLIHALEKFEPERGFRFSTYATWWIRQGVERAVMN
                     QARTIRLPVHVLRELRHMTYARRHLEAESESGAEIRVEDIAHLTGRPVEVVHDVLQLG
                     AWPASLDAPLDADPDSTLGDLLSDVASEVPETTATRHELERRVHVWLERLDERHRHVI
                     ERRFALEHQSYATLDELADELGLTRERVRQMQLEALKQLRQTLAAEHVGRDAVL"
     gene            complement(7112..7474)
                     /locus_tag="VLN25_10755"
     CDS             complement(7112..7474)
                     /locus_tag="VLN25_10755"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HSQ11072.1"
                     /translation="MQWLWPAPGKVIENFDETRNKGLDIAGNEGDPVQAAGDGEVVYV
                     GSALRGYGNLVIVKHNDEFISAYAHNRQILVKQGQAVKRGQRIAEFGRSDADRVKLHF
                     EIRRHGKPVDPLRYLPVR"
     gene            7497..>7690
                     /locus_tag="VLN25_10760"
     CDS             7497..>7690
                     /locus_tag="VLN25_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11073.1"
                     /translation="MTVSGGTVAAPGTTGGGWGEGVDGIAGKIGAAGGNVAPGAVATG
                     GDASAPGRGRTSAYPTGATGV"
BASE COUNT         1345 a         2559 c         2445 g         1341 t
ORIGIN      
        1 gttgcggcag gccacttcga tcatctgtgc aaagttgtcc tcgcgccgca cgcccttgcc
       61 gacgttgccc ggattgatgc ggtacttcga cagtgcctgc gcacacccgg gatgctgcgt
      121 gagcagcttg tgcccgttgt aatggaagtc gccgatcagc ggcacgtcga tgcccatgcg
      181 atcgagctgc tcgcggatcg cggccacttc ggctgcggcc tcgggcgaat tcaccgtgat
      241 gcggacggcc tcggatccgg cctgagccag ttccttaacc tgaacggcgg tcgcgatcgc
      301 atcggccgtg tcagtgttgg tcattgactg gaccagcacc ggagcgtccg ctcccatttt
      361 cacgacacgc gcgccccagc gcagttcgac ctgccgagtc ggccgtctcc gggccgggcg
      421 agccgcggcc ggcagacagt cgagaggttc gatcggctgg ttcatcccga tctcagttga
      481 tcgtgaagcg agccacgtac tgctttgtgt gaggcaccat gtcgaaaacc tcgccacgta
      541 cttcgaccgt cgtcttggtg gcatcaccaa tgacgacgta gaacgggggc gtgccttcga
      601 tgacgaattc ggcgccgggc gagttgtgct gtgagtgcag caccttgccc tcggcgtcct
      661 tgacctcggc ccacgagggc ccggcgaaac ggagcttcag caacggcggt ggtgcaacgg
      721 gctccatgac gcccgcgctc tcaccggccg aacccgccga ccctgccgtg gcgggcgctg
      781 catccgcccc tagcgctgac gcgaccgcag gaacgacagc cggcggggtc gtcgcgacag
      841 ggggcgccgc ggccagcttc gtcggcgaca cggatgcacg cgtttccgct tgctgacggg
      901 tcgcgtacca gcccagagcg cccagcacaa tcagcaccag caccgccccg agcacgacga
      961 cgcggcgcga cgcctgctcc cgcgcagcgg cccgaaccgg tgagtagtcc cgcggctggg
     1021 ccatgccgtc gacgaccgaa cgttcggccg gtaccatttt cgtggacaga tcgtccagca
     1081 gcggcaccgg atctgcattg acgatgcggg cgtagctgcg cacgaacccc cgcacatagg
     1141 ccggctcggg cagcgcggcg agcgactcct gctcgatggc ccggatctgg ttcggatgca
     1201 ggcgcagtcg cgcagcgacg tcactgaccg tcagtccggc ctgctcgcgt tcccacttca
     1261 ggcgcgcacc gaaggaagtg ggccgcgacg gcgcctcggc cgccgtagac gcctgggcgt
     1321 ccacgcctgc cgcggccgtg tcggcgtcgt cactcatcga atgccccgcg cgcgagcatg
     1381 ccggcctcct tggaaccggg gaaccgggtg cgcagctcac tggccagtcg cgtttccgtg
     1441 cgcagatcgc cctgtgcgcg cgcgaccttc agtccaagcc agaccgtctc gggcgaggcc
     1501 tcggcggatt tcaccagcag cccgtagtaa aacgaggcgc gatccgtctg cccggtcgcc
     1561 aggtaaaggc gagcgagttc atatttggtg gtgacgctgg tggcatcgag ctcgaacgac
     1621 cggcgcaggt agggctcggc gccggggtag tccttgacac gaagcaggca gtgacccgca
     1681 ttgaaatttg cgcgtgcggg cgtagcgtag agaggatccc gcagtgagcg ttggaagtac
     1741 gcgatcgatt cgccttctcg gccggtctgg cacagatacc acccgtagtt gttgttcagg
     1801 tcggcatttg acggatcgag ctgcagcgca cgctggaaat tctcctcggc ggcccgccgg
     1861 tcttcgagtt ccatgtaaat caggcccagc agtccatagg catccgcgaa ggtcggatca
     1921 atctggacag cgcgcttggc ttcctcgagc gccacggcca gttgtccgtt gcggaagtaa
     1981 ccggccgcga gttccacccg cgcgcgtgcg cggctcttcg tgaccgcagg cgacgcaccc
     2041 gcggcaccgg attgcgtcgt cgacaccacc tcaccactgg aagtcgtgac ggtggtggtc
     2101 gtgcatgcag ccaggatgcc agcacaggcc acaacgatga gttgcgcgat cagtccacct
     2161 cgcatgccta gcctcccggg gataccgtta tcggccatcg gcgactgcga tgggtattgc
     2221 acgttcaggg gccacgcgtc gagtgcggtc ctgcacctca cccgccagtt ggccacaagc
     2281 cgcatcgata tcgtcaccgc gggtgcgacg caccgtcgtg acgatgcccg catcggccag
     2341 tcgctgcgcg aacgagcgca cacggtggac cggggaccgc gaaagtccgg cgtcagggaa
     2401 ggcattgaac gggatcagat tgaacttgca cggaacatcg cggaccagcg cgaccagttc
     2461 gtcagcgtgt tcatcggcgt cgtttacgcc atcgagcatc acgtactcga aggtgatgaa
     2521 gtcgcgcggc gcgacgcgca ggtagcgtcg gcaggcagcc atcagatcgc gcagggggta
     2581 cttgcggttg aggggcacga gttcgtcgcg cagcgtgtca ttcggcgcat gcagcgacac
     2641 tgccagcgcg accggacaat cggcaccgag gcgatcgatc tgcggcacca tgcccgacgt
     2701 cgataccgtc acgcgccgac gcgacagccc gtaagcgttg tcgtcgagca tcaatcgcag
     2761 cgccggcagg agggcttcgt aattctgcag cggctagccc atgcccatca tgaccacatt
     2821 gctgatgacg cgttcgcctt cggtgtccag ccccagtgag cgccgcagcg cgtgctcggc
     2881 ccaccacagc tgaccgacga tctcggccgt ggtcaggttg cgactgaagc cctggcgccc
     2941 ggtcgagcag aagacacagt tgaccgcaca gcccgcctgc gatgacacgc acaaggtgcc
     3001 gcggtcatct tcaggaatga agacggtctc gatggcgttg ccggcaccga catcgagcag
     3061 ccacttccgc gtgccgtcgg acgaggtgga gtcccgcacc acggccggcg ccacgatcgc
     3121 cgcgtgctcc gcaagctttg cccggagtga cttggccagg tccgacatcg cgtcgaagct
     3181 cccggcaccg cgtcgatgaa tccagcgcgc gagctgtctc gcgcggaacg gcttttcacc
     3241 gagagcctcg gtgtattccg ccagcgccgc aacatcgagg ccgagaagat tgacgcgttc
     3301 catcaacgtt tcgctcagcg cgagtacacc gccatttgag ggaagaagta cgcgatctcg
     3361 atgcgcgccg tgtcttcgcc gtcggaaccg tgcacggcgt tggcatcgat gctctccgcg
     3421 aagtccgcac ggatcgtgcc agcggcggcc ttcttcgggt ccgtcgctcc catcagttct
     3481 cggttttttg caatggcgct ttcgccctcg agcacctgga tcatgaccgg cccggacacc
     3541 atgaaatcca ccaggtcctt gaagaacggt cgttcccggt gaaccgcata gaagccctcg
     3601 gcttcgtggc gcgacagtcg cgccatgcgg gccgcgacga ccttgagtcc gtttgtctcg
     3661 aaccgcgagt agattttccc gatgacattc ttcgcgacag cgtcgggttt gatgatcgac
     3721 agagtgcgtt ccaccgccat gacttcagcc tttagttttc tcagacgttt tcttgatcgg
     3781 accagtccgg ccgccgtgga ccacggcgtg ataccgcgga gagattccgg gcagccaaac
     3841 cgaaaacctc gataaatgaa acacttgcaa catcgaagtt agccaaaaat tttaacatgc
     3901 gacggatcgc cagcggcgat cggcgccccg ggggttcagg ggcgagccag gccgggttcc
     3961 agcatggcga tcgactccac gtgggaggtg tgcgggaaca tgttgacgat gcccgcggcg
     4021 cgcaggatcc agtctccctc atgaaccagg acggcgcagt cgcgcgccaa tgtcgctgga
     4081 ttgcacgaga catagaccac ccttgccggc ggccgctcag tcaccgccag cacctgcacg
     4141 atcgcgagcg caccttcgcg gggcggatcg atcagaactt tgtcgattcc gccgcggcgt
     4201 tcgacagccg cttcccagtc ggtcgcgctc cactggaaca gattgcgcgc ctcaaaggat
     4261 gtgcaatcgc ccagtgcgtt ggcgtcggcc gccgcgcggg cccgctccac cagattcgaa
     4321 ctgccctcgt agcccaccac atgccgggcc cgtgtcgcga gcggcaaagt gaaattcccc
     4381 aggccacaga agaaatccac gacgctctcc tgcggcgtcg gcgccagcag gcggacggca
     4441 cggctgacca gcgcctcgtt gacgctatgg ttcacctgag tgaagtcagt tggactgaat
     4501 gacagcgtca ctccgaactc gggcaaccca agccgcaatt ccgacggttg ctccactgtc
     4561 agcggcaccg ccgtgtgcgg tccgccaggc tgcagccaga actccacgga atgccgatcc
     4621 gcaaactcgc gcagacgggt ccgatcgtcc gtcgtcggct cctgcagcac acgcaggacc
     4681 agcacgacct gaaggccatc agtccgatcc gccgcggcca gttcgatctg cggcaaccgg
     4741 tcgcgcagcg acagcgactc caccaggcgg cgcaatggca ccagcagatc gctgacgcgt
     4801 acgggcagga cctcgcagcg ggtcatgtcg gcaacaaagc tgcttgcgcg ctcatgaaat
     4861 ccgaccagga cctcgccctt cttggcgacg tcacgaacag tcaatcgcgc ccgatagcgg
     4921 tagttccatg tcggtcccgc gatgggtcgc agcatgcgct ccgcacgcag gcgtccgata
     4981 tgccacaggc tgtcttcgag tacacgctgc ttgatcgcga cctgcgcacg cgcatcgagg
     5041 tgctgcatcg agcaaccccc gcacgaaccg gcaccgagcc cgaagtgagg acaccggggc
     5101 gtggcacgaa gcgaggaaac ccgctcgatg gcgaccgtcc ggccgacctc gtagctggcc
     5161 ttgttgcgcc gtcgttcata ggtggcttgc tcacccggca gcgcgccctc gacgaataca
     5221 gccttgcctt cgcgatgccc gacgccacga ccctcctggt cgagggactc gatctcgacc
     5281 tggaagatct ccccggactt catctcaggt tcgtccccag gccgccaggt attccttcca
     5341 gtggggctgt ccaagcgcgg tgagcgcggc gtgtacgcgg ccttcttctt cggcgtagcc
     5401 gccgtcatcg aggtttcctc gcatgcgctg gaagcgcgcg aaaagcaggt aggtgttgac
     5461 cacgtcggtc tcgcagtacg cgcggatctg ctcgatgcct ccggcctggt acgcctccca
     5521 gacacggctg ccatccatgc ccagttttcc ggcgaatcca cacagcttcg ccaagtcgtc
     5581 gagcggagcc gaagcgcgcg gctgatacat cgccaacaga tccatcaggt cgatatgacg
     5641 tgtgtggtat cggccgatgt agttgttgta gcgaaagtcg cgatcgtcat cgccgagttc
     5701 ccagtagcgc gtcgcatgca ctccatggat gaggccgcga tagtgcagca ctggcaggtc
     5761 gaacccgccc ccgttccagc tgacgagctg cggggtgtag cgatcgaccg ttgcgaagaa
     5821 ggcctggatc agctttccct cgtcgtcgtc ggggtgcccg atcgagcgga cacgaaaccc
     5881 ggagtcgtcg cggaacacgc aggaaatcgc gacgactcga tggagatgca acggcaggaa
     5941 gtcgctgccg gttttctccc gacgctcatc gaagagccgc tgtgcaacat cgggatcgct
     6001 cagcccggaa aactgcgcac gcagttttct ggcaccggcg atgtcgggta cggtctcgat
     6061 gtcgaaaacg aggatcggcg tcatcagcga aagggcatcg cgaccttgcg atctacagca
     6121 ccgcgtcgcg accgacgtgc tcggcggcga gggtctggcg caattgcttc agtgcctcga
     6181 gctgcatctg ccgcacgcgc tcgcgcgtca ggcccagttc gtccgcgagt tcatcgagcg
     6241 tcgcgtagga ctggtgctcc agggcaaagc ggcgctcgat cacatggcga tgccgctcgt
     6301 cgaggcgttc cagccagacg tgcacgcgcc gctccagttc gtgccgcgtc gccgttgtct
     6361 ctggcacctc ggacgcaacg tcggacagca ggtcgcccag cgtggagtcc ggatcggcat
     6421 cgagcggcgc atccagtgag gccggccagg cgccgagctg cagcacatcg tggaccacct
     6481 cgaccggccg gccggtcagg tgtgcgatgt cttcgacccg gatttcggcg ccggactcgg
     6541 attcggcctc caggtgacgc cttgcataag tcatgtgtct caattcgcgc agtacatgaa
     6601 ccggcaggcg gatagtgcgg gcctggttca tgacggcacg ctcgaccccc tgacggatcc
     6661 accaggtcgc gtacgtcgag aagcggaaac cccgctcggg ctcgaacttc tccagggcat
     6721 ggatcaatcc gagattacct tcctcgatca ggtcgagcag aggcagtccg cgatgcgcgt
     6781 agcgctttac gatcgacacc acgagccgga gattgcgctc gatcatctgc tgccgtgctg
     6841 caaagtcgcc cgcgcgtgcg cggtcggccg tggcacgctc ttcttcagcc gacatcagcg
     6901 gatgggttcc gatgtccgca aggtagcgct gcacgttgtc gggctcatag tcgtcgtcgg
     6961 aagcttccgc ttccataccg ttcagcgcgc gagcaaattc atcgctttca ccgggcgcgt
     7021 ctcgctcgaa agcgctgcgc tccgaggcgt ttgcgtcgtc cggctcgcag gccggcgtgt
     7081 ctggaatctc gcggataccg gccatgcctg ctcagcgcac cggcaggtag cgcaacggat
     7141 cgaccggctt gccgtgacgt cgtatctcga aatggagctt gacccggtct gcatcgctac
     7201 ggccaaactc ggcaatccgc tggccgcgct tcaccgcctg cccctgcttg acgaggatct
     7261 ggcggttgtg cgcgtacgca gaaatgaact cgtcattgtg cttgacgatg accaggttgc
     7321 cgtagccgcg caacgcgctg ccgacgtaga cgacttcccc atcaccggct gcctggaccg
     7381 ggtcgccctc attgccggcg atatcaaggc ccttgttgcg cgtctcgtcg aagttctcga
     7441 tcacctttcc gggcgccggc cacaaccact gcactgagct ctcaagcggc gccggcttga
     7501 ctgtctccgg cggtacggtg gcggcgccgg gcaccacggg cgggggctgg ggcgaaggcg
     7561 tcgacggcat cgcaggcaag atcggcgcgg ccggcggcaa cgtggcgccg ggcgctgtcg
     7621 cgaccggtgg cgatgcatcg gctcccggca ggggccgaac ctccgcatat ccgaccggcg
     7681 ccaccggggt
//
LOCUS       DATIRT010000473        20388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1164188, whole genome shotgun sequence.
ACCESSION   DATIRT010000473 DATIRT010000000
VERSION     DATIRT010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 20388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20388
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164188"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1427
                     /gene="argS"
                     /locus_tag="VLN25_10765"
     CDS             <1..1427
                     /gene="argS"
                     /locus_tag="VLN25_10765"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HSQ11074.1"
                     /translation="RERECFGATTAHVGHRVMIEFVSANPTGPLHLGHARQAAIGDAL
                     ANLLAAQGWLVSREFYYNDAGVQIANLALSVQARARELLGESVEFPEAGYRGDYIVDI
                     ARDFLAKRTVKARDGEPVTASGNPNDLEALRHFAFVYLRNEQDADLATLGVAFDNYFL
                     ESSLYSDGKVDATVKALVDSGMTYEAESALWLRTTEFDDLAGHGGPPKDDKDRVMRKS
                     DGTYTYFVGDVAYHLNKLQRGFEKVINIQDTDHYGTAARVRAGVQAAARSLELSAPRG
                     YPDYLLHKMLRVTKGGEEVKMSKRAGTYVALRDLLEWVGRDATRYFLVARKADAEFTF
                     DVDLALSQSEDNPVYYVQYAHARICSVLQQAGEKGYTVPDDAAALRTELGPLASPREQ
                     SLLNRLSAYPDVLADAANDLAPHQLANYLKDLAADFHGYYNAERVLVDDDGVRNARLA
                     LLLATRQVVRNGLALLGVSAPEKM"
     gene            1427..2008
                     /locus_tag="VLN25_10770"
     CDS             1427..2008
                     /locus_tag="VLN25_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HSQ11075.1"
                     /translation="MPRLQPDFATSDFVSRQTGGTLLGFVLGLIFGLAIAVAVALYIT
                     NAPVPFLNKVRPASENINPGSGGQLPDPNKPLQSAPQPADTATVPPGRLDAATDPKAA
                     PPPPEKGMAALPDDGSRYLLQAGAFKTPEDADAMRARLALLGFDAKIFPRDQDGQTLY
                     RVRLGPYGNVEDVNRMRKTMAENGIDVQLIKVR"
     gene            2124..2780
                     /locus_tag="VLN25_10775"
     CDS             2124..2780
                     /locus_tag="VLN25_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HSQ11076.1"
                     /translation="MPLSRRLLLIASAATAATPLLAHAQAAPRVPQDGKDYVTLKTPV
                     PVESSGKVEVLEFFQYSCPHCATYDPYLQAWKKKLPADVDYKRIPIAWDNSTMPHVRI
                     YYTLETLGKLDDMHQKVFTAIQKNRQPMVNPEQIADFMAANGIDRKQWIDTYNSFSVA
                     ARSNRAGQIWRAYKIDGTPAVAVDGKFTTAPSMVGSREGSLVVLDYLVQRARADKSKK
                     "
     gene            2906..3667
                     /locus_tag="VLN25_10780"
     CDS             2906..3667
                     /locus_tag="VLN25_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016475352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSQ11077.1"
                     /translation="MNVFITGASSGIGAALAKELAGRGACLGLVARRREALEALVATL
                     PGQHHLYAVDVTDTDAIIAAGRAFDTATGGADIVVANAGISVGMKTEHYEDLAQLRRV
                     FDTNVFAMAGTFHPFIAPMKARGRGTLVGIASVAGIRGLPGSEAYCASKSAVITYCES
                     LRVELSKAQVKVVTISPGFVRTELTAKNPYSMPFLMDAADFAPRAVDAVLAGVRYRTI
                     PWQMGVVTTLLKFMPRALFDLLTRGRKQKPRATAA"
     gene            complement(3678..4871)
                     /gene="metC"
                     /locus_tag="VLN25_10785"
     CDS             complement(3678..4871)
                     /gene="metC"
                     /locus_tag="VLN25_10785"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HSQ11078.1"
                     /translation="MKRRRVTDLVTAGRDPRTSFDFVNPPLVRGSTVLHESVADMKAR
                     IARRNAGEDGASVTYGIYGTPTHHAFYEALNALEGGHASWALPSGLTACTIPILAYVQ
                     AGDHVLVPDSVYWPTRRFCRDTLARYGVEATFYDPRIGEQGPAAVEAMFRPTTRVLFL
                     ESPGSLTFEMQDVPLLAEVARRRGAVSMMDNTWATPLFFQPLAHGVDVSIHAATKYIC
                     GHSDVIMGTVTSNASAWPRMRETIHHLGLTTSPDDVYLAHRGLHSMAARLRQHTATAW
                     RLIEWLQSKPEVARILYPALESDPGHALWKRDMSGASGLFGVVLRPVAEDRFIAFIDG
                     LELFGRGYSWGGFESLLIPVFPERTVVPLSFEGPLFRIAAGLEDADDLIDDLASGFER
                     LRGSA"
     gene            complement(4887..5987)
                     /locus_tag="VLN25_10790"
     CDS             complement(4887..5987)
                     /locus_tag="VLN25_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSQ11079.1"
                     /translation="MNPNEHELHYPWADVPGPGAWTTLRRGVHWVRMPLPFALDHINL
                     WVLDDEIDGRRGFTIIDSGITSDETKSAWERIFDGLFGEQPLLRLLCTHFHPDHLGLA
                     HWLTAGGDRTRWQAPLWMSATEYAFGRFLAAGGDDTSGEGAAAHFRRHGLVDDESLAR
                     VRARGSGYYPKLVPSVPLRFRRLLDGDEVAIGPVHCKRVFRIIVGYGHAPEHVSLYCD
                     EDRLLIAGDMVLPRISTNVSVFDVEPEGNPLPRYLHSLDRLLLLPADTLVLPSHGKPF
                     TGLHQRIAQQHTHHRERLAEVLSACASPQTAASIVPVLFKRTLDLHQTTFALGESLAH
                     LHALWYEGAVERVERNGVFEFQAAPTRPHAVQ"
     gene            6080..6526
                     /locus_tag="VLN25_10795"
     CDS             6080..6526
                     /locus_tag="VLN25_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HSQ11080.1"
                     /translation="MSTRARAASVRSTPAIDEPEARSYSITELAREFDITPRAIRFYE
                     DQGLLAPARSGTSGLTRVYSPRDRTRLKLTLRGKRLGLSLLQVRELIDMYESPKDATA
                     QAKRFLSVLNQHRTALEQQREDIQVTLAEIAQHDAECRRLLERGRR"
     gene            6656..6802
                     /locus_tag="VLN25_10800"
     CDS             6656..6802
                     /locus_tag="VLN25_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11081.1"
                     /translation="MNAIPIKLSPPLNEPEPVPRDLTAAHGITWSVGLGAVVWAVLLF
                     ALLD"
     gene            complement(6811..8655)
                     /gene="recQ"
                     /locus_tag="VLN25_10805"
     CDS             complement(6811..8655)
                     /gene="recQ"
                     /locus_tag="VLN25_10805"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HSQ11082.1"
                     /translation="MDRANPGTLLKDVFGYDSFRGFQREVIEHVGAGGDALVLMPTGG
                     GKSLTYQIPALLRSGVGIVVSPLIALMADQVAALDELGVRAAALNSTLSAADAAAIER
                     RMRSGDIDLVYIAPERLLTERCQRLLEQTQAGAGIALFAIDEAHCVSQWGHDFRPEYG
                     ALAILRERFPAVPRIALTATADDPTRHEIVERLLVDPRVFVASFDRPNIRYRIVEKRE
                     PREQLLRFIRDEHPGEAGIVYCLARNTVDEVAHFLAGHGIDALPYHAGMDALARAANQ
                     ARFQRDDACVIVATIAFGMGIDKPDVRFVAHLDMPKSVEGYFQETGRAGRDGLPADAW
                     MAYGLADVVQQRRLIEQSDAEDTYKRLSGAKLDAMLALAEAADCRRVRLLAYFGEASA
                     PCAQGQNACDNCLAPPALRDATEDARKLLSTIYRCQTAGGFGFAATHVIEVLRGKVTD
                     KVTRFGHERLSTFGIGADVPEAEWRLLLRHLVAQHIVEVDHDHYNVLRLTEASRAVLR
                     GDRHIELRVQLHEPRRRAKGARVAAAEGLDGAAARVFARLREWRAGVAKEHGVPAYVV
                     FHDGTLRTVAAERPRSLEALGQVSGIGAAKLERYGAAVLQLVGDDGNG"
     gene            8762..9190
                     /locus_tag="VLN25_10810"
     CDS             8762..9190
                     /locus_tag="VLN25_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11083.1"
                     /translation="MTADASSAVPSSREFTMLGITYALYTIGLVMFWPAFIGLIIAYI
                     KRNDVAESFLRSHYGWLIRTFWWWMAWFALAIAGMLIMVLPSAIEMGHMIKATDIVRV
                     PWEVLGGAIAGGLAITVVWFWVVYRLVRGTLRLADGRAVP"
     gene            9378..9761
                     /locus_tag="VLN25_10815"
     CDS             9378..9761
                     /locus_tag="VLN25_10815"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HSQ11084.1"
                     /translation="MRLIGARLTTVEPGRCVIELPVRDDLTQQHGFVPGGVVGMIADS
                     AGGYAGFTLMPADASVLTVEFKINMLAPAAGDLLIATGEILKPGRTLAIVRADVMALR
                     GDRQTRVATMQQTLMVMHGVADEKI"
     gene            9772..10950
                     /locus_tag="VLN25_10820"
     CDS             9772..10950
                     /locus_tag="VLN25_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012760965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HSQ11085.1"
                     /translation="MQLPGLKFQLGEDIDALREAVHDFAQAEIAPRAAEIDRSDQFPM
                     DLWRKLGDLGVLGITVDEDYGGAAMGYLAHIVAMEEISRASASVGLSYGAHSNLCVNQ
                     IKRNGTATQKAKYLPKLVSGEHVGALAMSEPNAGSDVVSMKMRADFKGDRYVLNGTKM
                     WITNGPDCDTLVVYAKTDPQMGARGMTTFIVEKGMKGFSVAQKLDKLGMRGSHTGELV
                     FQNVEVPAENVLGAEGGGAKVLMSGLDFERAVLSGGPLGIMAACMDAVVPYVHDRQQF
                     GQSIGEFQLMQGKLADMYTTMMACRAYVYEVGRACDLATPETVRHLRKDAAGAILYSA
                     EKATWMAGEAIQALGGNGYINDYPTGRLWRDAKLYEIGAGTSEIRRMLIGRELFAETM
                     "
     gene            10960..11472
                     /locus_tag="VLN25_10825"
     CDS             10960..11472
                     /locus_tag="VLN25_10825"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HSQ11086.1"
                     /translation="MHPWQQHLDQLRAGFKPPGLADADTLKHISGLEFLRRMIRGEIP
                     TAPISQTLDFYLIEADPGRAVFQGRPTHAHYNPIATVHGGYHATLLDSAMGCAVQTLC
                     EAGRAYTTLEFKIHCVRPLTELTGPVRAEGKVISSGRRMATAEGRLVDSEGRLYSHGT
                     TTCMLFDLQS"
     gene            11483..12487
                     /locus_tag="VLN25_10830"
     CDS             11483..12487
                     /locus_tag="VLN25_10830"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSQ11087.1"
                     /translation="MNDVHALDNSVEKSGAVRQYWTDFCVATRLDPATPFQAWYFGDT
                     PALAHELAELVLHGPKRATAGLAEFNDQMPQVKPMPGGYSVLTEHDGTPRAVIRTTVL
                     ERRAFADVDASFAWDEGEGDRTLADWKDGHRRYFTRELATIGRDFDETQEVDLERFEL
                     LYPFDRALHPVDCGPRLLPCLLPGALASSAALQSQYYAREHGFGVFFEAGRMADIAAF
                     LSRYDVQRDGVWVAVDSGQVIGTIVIDGGASADRTQAQLRWFIMADTLRGKGMGRRLM
                     QAAMGFAVARYARVWLGTFDALHAARHLYEGFGFRKVLERPSTEWGPEVREQHWEWIR
                     "
     gene            12475..13134
                     /locus_tag="VLN25_10835"
     CDS             12475..13134
                     /locus_tag="VLN25_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HSQ11088.1"
                     /translation="MDKVTVFGLGLDKLTLFALAVLLLALTPGPVWIYLISRTLTQGR
                     RAGYFSLIGVMAGVLVHLMAAALGLSALLLALPAAFDAIKLAGAAYLLWLAWSTLRGS
                     GFSFTPQPLDPVPDRVLFRQSLTASVVNPKVAVFYLSLFPQFVDPASGPVLLQSLALG
                     IVHVTVSTLVDGALVTVAGALSVWIARRPGWLRMQRWVLGSAFGALAVWLAATPRHSP
                     H"
     gene            13152..14957
                     /gene="aceK"
                     /locus_tag="VLN25_10840"
                     /pseudo
     CDS             13152..14957
                     /gene="aceK"
                     /locus_tag="VLN25_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797380.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            14967..16148
                     /locus_tag="VLN25_10845"
     CDS             14967..16148
                     /locus_tag="VLN25_10845"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HSQ11089.1"
                     /translation="MNDPIVIVSAARTPIGGLLGDFTSLQATQLGAFAIRAAVDRAGI
                     AGDAVDEALMGCCLMAGQGQAPARQAVLGAGLPQSVGAVTLSKMCGSGMRAMMFAHDM
                     LVAGSADVVLAGGMESMTNAPHLLVGSRRGYRYGAFTTYDHMALDGLEDAYERGKAMG
                     VFAEQCVAKYSFTREEQDAFAIASTERALKANTDGSFAWEIAPVTIAGKGGEVKIDKD
                     EQPFKAKLDKIPGLKPAFKKDGTITAANASSISDGAAALVLMRQSTAAKSDRKPIALI
                     RAHAVHAQAPDWFTTAPIGAINKVLEKTGWKPGDVDLWEVNEAFAAVTMAAMRELKLP
                     HDKVNIHGGACALGHPIGASGARIVVTLLGALRKTGGKRGVAALCIGGGEATALALEL
                     L"
     gene            16233..16868
                     /locus_tag="VLN25_10850"
     CDS             16233..16868
                     /locus_tag="VLN25_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HSQ11090.1"
                     /translation="MLGVQDLGLFVVTGLVLNATPGVDMAYAVTRTLQYGGRGGVAAA
                     LGISAGCTVHAVAAALGLAALLAASAWAFSVIKWAGALYLLWLAWGMARAAWRPGQPA
                     EAIALPASTPRSRIFVQGFLTNVLNPKVALFFMALLPQFIGADAPNKPMAFLFLGAVF
                     IFNGTLFLLLLVVLAQRLRRVGTRPAVRRVLNGLGAALFAGLATRLALAQR"
     gene            16878..17552
                     /locus_tag="VLN25_10855"
     CDS             16878..17552
                     /locus_tag="VLN25_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014895885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSQ11091.1"
                     /translation="MATVLIIGASRGIGLEFVRQYAADGARVIATVRRSEDTARLREL
                     GAKPLLLDVVDEATVAEFGVQLHGEKIDVAIYNAGVYGPRTSAIATPGGLDFDAVMHT
                     NVRAPMQLIPQLVPGLAAAGGKLALISSRMGSISLMSSTSGWLYRASKAAANAALRAM
                     SLELGPHGIVCIAFHPGWVRTEMGGAGADIDAATSVTGMRRVLASANASQNGKFLNFN
                     GEQLSW"
     gene            17557..17949
                     /locus_tag="VLN25_10860"
     CDS             17557..17949
                     /locus_tag="VLN25_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HSQ11092.1"
                     /translation="MNDKRLISSGSAFEAVAGYSRAVVEGDYVHVAGTTGFDYAAMTI
                     DTDAMAQTHQCFRNIASALEQAGSSLDDVVRVRYFLTDAALFEQLAPVFGKYFSTARP
                     AATAIVCGLVDARMKIEIEVTARRRAWS"
     gene            18000..18494
                     /locus_tag="VLN25_10865"
     CDS             18000..18494
                     /locus_tag="VLN25_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11093.1"
                     /translation="MPLQVFIVLVYGICSWLAIAIIEPVMRSFANEAGMESWLFIAIP
                     GLLAMLFAVLSYIRAATRVTNMEQSMSRALLVALLTWIAITTLIAWLWCPAHSALRCF
                     SNALITTGIVGGGPLLVGAMVAGGIVALVLRQRPSWISFGERRVLMAPDADPNPTSKP
                     GNAR"
     gene            18484..19608
                     /locus_tag="VLN25_10870"
     CDS             18484..19608
                     /locus_tag="VLN25_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSQ11094.1"
                     /translation="MLAEEHLLLREALRDFASNRLAPHAAEWDRNATFPKEALKELAA
                     LGTFGIAVPEEYGGAGMDYSALAVALEEIAAGDGATSTIISVNNCPVCSILMAWANEE
                     QKQRYLVPLARGEYLGAFCLTEPHVGSEASGLRTTAVRDGDHWILNGVKQFITSGKHA
                     DVAIVLAVTDKAAGKRGISAFIVETSTPGYVVARIEEKLGQHASDTAQILFENCRVPA
                     ANLIGDEGAGYRIALSGLEGGRIGIAAHSLGMARAAYEAALKYARERIAFGQPIFDHQ
                     AVQFRLADMATQLEAARQLIHHAAALKDAGQPCLKQAAMAKLFASEMAERVCSDAIQI
                     HGGYGYVSDFPVERIYRDVRVCQIYEGTSDIQRILIARNL"
     gene            19757..>20388
                     /locus_tag="VLN25_10875"
     CDS             19757..>20388
                     /locus_tag="VLN25_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HSQ11095.1"
                     /translation="MRVILAGATGWAGSAIARGIARADDLQLVASVARKAAGQNLAAV
                     LGEPALSAPVLATAAEALATPCDVYVEFTKPAGAKANILAALDAGVHVVVGTSGLSDV
                     DYAEIDGVARARGRGVLACGNFALTVVLLQRFAEIAARYLDHFEIIDYAHEDKPDAPS
                     GTARELAFRLGQVKAPDPGVPLDQVRGPREARGATLNGVQVHSVRLPGYV"
BASE COUNT         3453 a         6755 c         6716 g         3464 t
ORIGIN      
        1 tgcgcgaacg cgagtgtttc ggtgcgacga ctgcgcacgt cggccaccgg gtgatgatcg
       61 aatttgtttc agccaatccg accgggccgc tgcacctggg ccacgcgcgc caggccgcga
      121 tcggcgatgc gcttgccaac ctgctggcgg cacagggttg gctggtctcg cgcgagttct
      181 attacaacga cgcgggcgtg cagatcgcca atctcgcgct gtcggtgcag gcgcgtgcgc
      241 gcgaactgct cggtgaatcg gttgagttcc cggaggctgg ctaccgcggc gactacatcg
      301 tcgacatcgc gcgcgacttc ctcgcgaaga gaaccgtgaa ggcgcgcgac ggcgagccgg
      361 ttactgccag cggcaacccg aacgacctcg aagcattgcg tcacttcgcc ttcgtgtatc
      421 tgcgcaacga gcaggacgcg gacctcgcaa cgctcggtgt cgccttcgac aactatttcc
      481 tcgaatcgtc gctgtactcg gacggcaagg tcgacgcgac cgtcaaggcg ctggtcgatt
      541 ccggcatgac ctacgaggcc gaatcggcgt tgtggctgag gacgacggag ttcgacgacc
      601 ttgccggcca tggcggaccg ccgaaggacg acaaggaccg tgtgatgcga aagtccgacg
      661 gtacctacac ttactttgtg ggggacgtgg cttatcacct caacaagctt cagcgcggct
      721 tcgagaaggt catcaacatc caggacacgg accactatgg cacggcggcc cgcgtgcgag
      781 caggcgtgca ggcggcggcg cgcagcctcg agctgtctgc gccgcgtggt tatccggact
      841 acctgctgca caagatgctg cgcgtcacga agggcggcga agaagtgaag atgagcaagc
      901 gcgccggcac ctacgtggcg ctacgcgacc tgctcgaatg ggtcggacgc gatgcgacac
      961 gctacttcct ggtggcgcgc aaggccgacg ccgagttcac gttcgacgtc gatctggcac
     1021 tgtcgcagtc cgaagacaac ccggtctact acgtgcaata cgcgcacgcg cgcatctgct
     1081 cggtcctgca gcaggccggc gagaagggct acacggtgcc cgatgatgcc gccgcgctgc
     1141 ggaccgagct cggcccgctg gccagccctc gcgagcagtc gctgctgaac cggttgtccg
     1201 cctaccccga cgtgttggcc gacgcggcga acgacctggc gccgcatcag ctggcgaact
     1261 acctgaagga tctcgcagcc gactttcacg gctactacaa tgccgagcgg gtgctggtcg
     1321 atgacgatgg cgtgcgcaac gcccggctgg cgctgttgct ggcgactcgc caggtggtcc
     1381 gcaacggcct ggccctcctc ggcgtctccg cgccggaaaa gatgtgatgc cccgactcca
     1441 acctgacttc gccacctctg acttcgtctc ccgccagacc ggcggcacgc tgctcggttt
     1501 cgtcctgggt ctcattttcg ggctggccat tgccgtggcg gtggcgttgt acatcaccaa
     1561 tgcgccggtg cccttcctca ataaggttcg acccgcgagc gagaacatca atccgggctc
     1621 gggcggacaa ctgcccgatc cgaacaagcc cctgcagtcg gcaccgcaac ccgccgacac
     1681 ggccaccgtg ccgccaggca gactcgacgc agcgactgat cccaaggcgg ctccgccgcc
     1741 gccggaaaaa ggcatggctg cgcttccgga cgacggttcg cgctatctgc tgcaggcggg
     1801 cgcgttcaag acgccggaag atgccgatgc catgcgtgca cgcctcgcgc tgctcggctt
     1861 cgacgcgaag atctttccgc gtgaccagga tggccagacc ttgtaccgcg ttcgcctggg
     1921 accctacgga aacgtcgagg acgtcaaccg catgcgcaag acgatggcgg aaaacggcat
     1981 cgacgtgcag ctgatcaagg tccgctgact gatactcgtc aatcctctcg tcggcgcggg
     2041 tgaacccttg tcatcgccgg cggtcgcaga ctcgttgaac cccaaacctg tcggcccccg
     2101 ggccgccttc tggagatcct gacatgccgc tatcccgacg cctcctgctg atcgcgtccg
     2161 ccgcgacggc agccaccccg ctgctcgcgc acgcccaggc cgctccgcgc gtgccacagg
     2221 acgggaagga ctacgtcacg ctgaagaccc cggtaccggt ggaaagcagc ggcaaggtcg
     2281 aggtgctcga attcttccag tactcctgtc cccactgcgc gacctacgat ccttacctcc
     2341 aggcctggaa gaagaagcta ccggccgacg ttgattacaa gcgcatcccg atcgcgtggg
     2401 acaacagcac gatgccgcac gtgcgcatct attacacgct cgagacgctc ggcaagctcg
     2461 acgacatgca ccagaaagtc ttcactgcga tccagaagaa ccggcagccg atggtgaatc
     2521 cggagcagat tgcagacttc atggcggcca atggcatcga tcgcaagcag tggatcgata
     2581 cctacaactc gttctcggtg gcggctcggt ccaatcgggc aggacagatc tggcgcgctt
     2641 acaagatcga tggcacgccg gccgtggcgg tcgacggcaa gttcacgacg gcaccctcga
     2701 tggttggttc gcgtgagggc agcctggtcg tgctggacta tctcgtgcag cgcgcccgcg
     2761 cggacaaatc caagaagtag tcaacctcac cccgagcgtc gatccgcacg cgcagcccgc
     2821 tggcgccgcc tcgcactgcc tcccgccatt cgccttcgca tctcccggct ttggcgtgaa
     2881 caggccctag actgtggcga tggcgatgaa cgtcttcatc accggcgctt ccagcggtat
     2941 cggtgcggca ctcgcgaagg agctggccgg ccgcggtgcc tgtctcgggc tggtcgcgcg
     3001 ccgccgcgag gcgctcgagg ccctggtcgc cacgctgccg ggtcagcacc acctgtatgc
     3061 cgtcgacgtg actgacaccg atgcgatcat cgcggccggc cgtgcgttcg atactgccac
     3121 gggcggggcc gacatcgtgg tcgccaacgc cggcatctcg gtcggcatga agaccgaaca
     3181 ctacgaggac ctggcccagc tccggcgcgt cttcgacacc aacgtgttcg cgatggcggg
     3241 cacattccac cctttcatcg cgccgatgaa ggcgcggggt cgcggcacgc tggtgggtat
     3301 cgccagcgtg gccggaatcc gcggcctgcc gggttcggag gcctattgcg cctcgaagtc
     3361 cgccgtcatt acctattgcg agagcttgcg cgtcgagttg tcgaaagcac aggtcaaagt
     3421 cgtgacaatt tcgccgggct tcgtacgcac cgaactgacg gcgaagaatc cctattccat
     3481 gccgttcctg atggatgccg cagacttcgc gccgcgcgcg gtggatgcgg tgctcgccgg
     3541 cgtgcgttac cgcaccatcc cctggcaaat gggagtcgtc acgacgttgc tgaagttcat
     3601 gccgcgcgcg ctgttcgacc tccttacgcg gggacgaaaa cagaagccgc gagccacggc
     3661 ggcgtaacga ggaccgatca ggcgctgccg cgcagacgtt cgaaacccga cgccaggtcg
     3721 tcgatcaggt cgtcggcgtc ctcgagtccg gcagcgatgc gaaacagcgg tccctcgaat
     3781 gacagcggga cgaccgtgcg ttcggggaag accgggatca gcaggctttc gaatccgccc
     3841 cacgaatagc cgcgaccgaa aagctccagg ccgtcgatga aagcgataaa gcgatcctcg
     3901 gcgaccggtc ggagcacgac tccgaacaga cccgaggcgc cggacatgtc acgtttccac
     3961 agcgcgtggc ccggatcaga ctcgagcgcc gggtacagga ttcgcgcgac ttcaggcttc
     4021 gactgcagcc attcgatcag ccgccatgcc gtcgccgtgt gctggcgcag ccgcgcggcc
     4081 atcgagtgca ggccccggtg cgcgagatag acatcgtccg gactggtggt cagccccaag
     4141 tggtgaatcg tctcgcgcat tcgcggccag gccgatgcat tgctggtcac cgtgcccatg
     4201 atgacgtcgg agtgcccgca gatgtacttg gtggccgcat ggatcgaaac atcgacaccg
     4261 tgcgcgagcg gctggaaaaa aagcggcgtg gcccaggtgt tgtccatcat gctgaccgcg
     4321 ccgcgacggc gtgcgacctc ggccagcagc gggacgtcct gcatctcgaa ggtcagcgac
     4381 cccggcgact cgaggaacag cacgcgcgtc gtcggccgga acatggcctc gaccgcggcg
     4441 gggccctgtt cgccgatgcg cgggtcataa aaggtcgcct ccacaccgta acgagcaagc
     4501 gtgtcgcggc aaaagcgacg ggtcggccag tagaccgagt ccggcaccag cacatggtcg
     4561 ccggcctgca cgtacgcgag gatcgggatc gtgcacgcgg tgagtcccga cggcagcgcc
     4621 cagctcgcat ggccgccctc cagcgcattg agcgcctcgt agaacgcatg gtgcgtgggt
     4681 gtgccgtaga tgccgtaggt gaccgacgcc ccgtcctcgc cggcattgcg gcgcgcgatg
     4741 cgcgccttca tgtcggccac gctttcgtgc agcacagtgg agccgcgcac cagcggcgga
     4801 ttgacgaagt cgaagcttgt gcgcgggtcg cgccctgcgg tgacgaggtc ggtgaccctg
     4861 cgtcgcttca tcgtgtcact ccggattcat tgaaccgcgt ggggccgcgt gggggccgct
     4921 tggaactcga acaccccatt acgctcgacg cgttccaccg cgccttcgta ccatagcgcg
     4981 tgcaggtgag cgaggctttc gccaagcgcg aacgtggtct ggtgcaggtc gagcgtacgt
     5041 ttgaacagca ccggcacgat gctggcggcg gtctgcggtg acgcacaggc agagagcact
     5101 tcggccaggc gctcgcggtg gtgcgtgtgc tgctgcgcga tgcgctggtg cagcccggtg
     5161 aacggcttgc cgtgcgacgg cagcaccagc gtgtcggccg gcagcagcag cagacgatcg
     5221 agtgaatgca ggtagcgcgg cagaggattg ccctcgggct cgacgtcgaa cacgctgacg
     5281 ttggtcgaga tgcgtggcaa caccatgtcg ccggcgatca gcagccggtc ctcgtcgcag
     5341 tagagcgata cgtgctccgg cgcgtggccg tagcccacga tgatgcggaa cacccgcttg
     5401 caatgcacgg gcccgatcgc gacttcgtcg ccgtccagca gtcggcgaaa gcgcagcggc
     5461 acactcggca cgagtttcgg gtagtagccg gaaccgcgcg cgcgcacccg ggcgagcgac
     5521 tcgtcgtcga ccagcccatg acgacggaag tgcgccgccg cgccctcccc gctcgtgtcg
     5581 tcgccgccag ccgcgaggaa ccggccgaac gcatactcgg tcgcactcat ccacagcggc
     5641 gcctgccagc gcgtgcggtc acctcccgcc gtgagccagt gagcgaggcc gagatggtca
     5701 ggatggaagt gcgtgcacaa caggcgcagc agcggctgct caccgaacag gccgtcgaag
     5761 atccgctccc acgccgactt cgtctcgtcc gacgtgatgc cactgtcgat gatcgtgaaa
     5821 ccgcgccgac cgtcgatctc gtcgtcgagc acccacaggt tgatgtgatc gagcgcgaac
     5881 ggcagcggca tgcgcaccca atgcactccc cgccgcagcg tggtccacgc gcctggcccg
     5941 ggcacgtccg cccaggggta gtgcagctcg tgctcgttcg ggttcatgcg gcggcgagtg
     6001 acagaggccg tgacttgacg ttgacgtcaa ctgcgacgat tctacgcggc ggatgccgac
     6061 gttcgaccac ccgacccgca tgtccacgcg cgcgcgcgcc gcctctgtgc gctccacgcc
     6121 agccatcgac gagcccgagg cccgctcgta ctcgatcacc gaactcgcgc gcgagttcga
     6181 catcacgccg cgcgcgatcc gcttctatga ggaccagggc ctcctggccc cggcccgcag
     6241 cggtacgtcc ggccttacgc gcgtgtactc gccgcgcgac cgcacgcggc tgaaactcac
     6301 gctgcgcggc aagcggctgg gcctgtcgct gctgcaggtg cgcgagctga tcgacatgta
     6361 cgagtcgccg aaggatgcca ccgcgcaggc gaagcggttt ctcagcgtgc taaaccagca
     6421 ccggaccgcg ctcgagcagc agcgcgaaga catccaggtc actctggccg agattgcgca
     6481 gcacgacgcc gagtgccggc ggctgctgga gcggggccgg cgctaggtgc gcacccggct
     6541 cccgctcccg ccgactggcc gaccaccggg cagggtgcgc atccggcccg ccatttacgc
     6601 aaggtttgat ccggatgaaa cccgccggca tttacggcgc gacaatgcca ccgctatgaa
     6661 cgccatcccg atcaagctca gccccccgct gaacgaaccc gaacccgtcc cgcgcgatct
     6721 gactgctgcc cacggcatta cgtggagcgt gggactcggc gccgtggtgt gggcggtact
     6781 tctgttcgcc ctgctcgact agggcctcct tcagccgttt ccgtcgtcgc ccactagctg
     6841 caggacggca gcgccatagc gttcgagctt cgcagccccg attcccgaca cctggcccag
     6901 cgcctcgagc gagcgcggtc gctcggcagc gaccgtccgc agcgtgccgt cgtgaaacac
     6961 gacgtaggcc ggtactccgt gctccttggc cacgccggca cgccactcgc gtagccgcgc
     7021 gaacacccgt gccgctgccc catccagacc ttccgctgcc gcgacccgcg cgcctttcgc
     7081 gcgccggcgc ggctcgtgca actgcacgcg cagctcgatg tgacgatcgc cgcgcaaaac
     7141 ggcccgactg gcctcggtga gccgcaacac gttgtaatgg tcatggtcga cttcgacgat
     7201 gtgctgcgct accaggtgcc gcagcagcag tcgccattcc gcctccggaa cgtcggcgcc
     7261 gataccgaag gtcgacaggc gttcgtgacc gaagcgcgtc accttgtcgg tgaccttgcc
     7321 gcgcagtacc tcgatgacgt gggtcgccgc gaagccgaag ccgccggccg tctggcagcg
     7381 gtagatcgtg gacagcagct tgcgtgcgtc ctcggtggca tcgcgcagcg ccggcggagc
     7441 gagacagttg tcacacgcat tttgtccttg cgcacacgga gcgctcgcct cgccgaaata
     7501 ggccagcagg cgcacgcgcc ggcagtccgc ggcttcagcc agtgccagca tcgcgtcaag
     7561 cttcgcgccc gacaggcgct tgtacgtgtc ttcggcgtcg gactgctcga tcaggcgccg
     7621 ctgctgcacg acgtcggcga ggccataggc catccaggcg tccgcgggca ggccgtcgcg
     7681 accggcacgg ccggtttcct ggaagtagcc ctcgacgctc ttcggcatat cgaggtgcgc
     7741 gacaaagcgc acgtccggtt tgtcgatgcc catgccgaac gcaatcgtgg cgacgatgac
     7801 gcacgcgtcg tcgcgctgga agcgcgcctg gttcgcggcg cgtgcaagtg catccatgcc
     7861 ggcgtggtac ggcagcgcat cgatgccgtg ccctgccaga aagtgcgcga cctcgtcgac
     7921 ggtgttgcga gccaggcagt agacgatgcc ggcctcgccc gggtgctcgt cgcggatgaa
     7981 tcgcaacagc tgctcgcgcg gctcgcgctt ttcgacgatg cgatagcgga tgttcggccg
     8041 gtcgaaactg gcgacgaaaa cccgcggatc gaccagcagg cgctcgacga tttcgtgccg
     8101 cgtcgggtca tcggcagtcg cggtcagcgc aatgcgcggc accgccggaa agcgctcgcg
     8161 caggatggcc agcgcgccgt actccggtcg aaagtcgtgt ccccactgcg agacgcagtg
     8221 cgcttcgtcg atggcgaaca aggcgatgcc ggcgccggcc tgcgtctgtt cgagcaggcg
     8281 ctggcagcgc tcggtcagca ggcgctccgg cgcgatgtag acgagatcga tgtcgccgct
     8341 gcgcatgcgg cgttcgatgg ctgcagcgtc ggccgctgac agcgtcgagt tcagtgccgc
     8401 cgcacggacg ccgagttcgt cgagggccgc gacctggtcg gccatcagcg caatcagagg
     8461 ggacaccacg atgccgactc cggaccgcag cagcgccggg atctggtacg tgagcgactt
     8521 gccgccgccc gtcggcatca gcaccagcgc gtcgccgccg gcgccgacat gctcgatcac
     8581 ttcacgctgg aagccgcgaa agctgtcgta gccgaagacg tccttgagga gggtaccggg
     8641 gttcgctcga tccacccgcg tagcgtagcg aaacgcggca gcggtacgat tcagccctca
     8701 attcggctgt gcgaccgaat gtgcctatgc cgatccttcg ccgtgccgcc tgcgagtgcc
     8761 catgactgct gacgcttcct ccgccgtgcc atcgtcccgc gagttcacga tgctgggcat
     8821 cacctatgcc ctgtacacga tcgggctcgt catgttctgg ccggcattca tcgggctcat
     8881 catcgcctac atcaagcgca acgatgtcgc cgaaagcttc cttcgcagcc actacggctg
     8941 gctgatccgg acgttctggt ggtggatggc ctggtttgcc ctggcgatcg ccgggatgct
     9001 catcatggtc ctgccgtcgg ccatcgaaat ggggcacatg atcaaggcga ccgacatcgt
     9061 gcgcgtgccc tgggaggtac tcggcggcgc gatcgccggc gggctggcga tcacggtggt
     9121 gtggttctgg gtcgtctacc ggcttgttcg cgggacgctg cggctggctg acggccgcgc
     9181 cgtgccgtag ccgacccggg actcgttgac gttaaatggg cggtaagttg acgttaacgt
     9241 caacttagcc gtagagtcat cggcccgcgg tcgccgccgc gatcacgctt cagattccgt
     9301 ccgcgccggc tgtgttcacc gcccccaacc ccgacttcga atcgcgcgtg cgcgactcgt
     9361 tcgcgcgcca ggctgcgatg cgactgatcg gcgcgagact gaccacggtc gaacccggcc
     9421 gctgcgtcat cgagttgccg gtacgtgacg acctgaccca gcagcacggc ttcgtgcccg
     9481 gcggcgtggt cggcatgatc gccgacagcg ccggcggcta cgccggcttc acgctgatgc
     9541 ccgctgacgc gagcgtgctg accgtcgaat tcaagatcaa tatgctggcc ccagccgccg
     9601 gcgacttgct tattgcgacc ggagagatac tgaagcccgg ccgtactctt gcgatcgtgc
     9661 gcgccgatgt catggctttg cggggcgacc ggcagacccg cgtcgccacg atgcagcaga
     9721 cgctgatggt gatgcacggc gtcgcggacg aaaagatctg aggagacacc aatgcaactt
     9781 cccggactga agttccagct tggcgaggac atcgacgcgc tgcgcgaagc cgtgcatgac
     9841 ttcgcccagg ccgagatcgc gccgcgcgca gccgagatcg atcgatccga ccagttccca
     9901 atggacctgt ggcgcaagct cggggatctc ggcgtactgg gcatcacggt cgacgaggac
     9961 tacggcggcg cggcgatggg ataccttgcc cacatcgtcg cgatggagga gatctcgcgc
    10021 gccagcgcct cggtcggcct gtcgtacggc gcgcactcga acctgtgcgt caaccagatc
    10081 aaacgcaacg gcactgccac gcagaaggcg aagtacctgc cgaaactcgt cagcggcgaa
    10141 catgtcggcg cgctggcgat gagcgaaccg aacgccggct ccgatgtggt cagcatgaag
    10201 atgcgcgccg acttcaaggg tgatcgctac gtgctcaacg gcaccaagat gtggatcacc
    10261 aatggcccgg attgcgacac gctggtcgtc tacgccaaga ccgatccgca gatgggtgcg
    10321 cgcggcatga cgaccttcat cgtcgaaaag ggcatgaagg gattttcggt cgcgcagaaa
    10381 ctcgacaagc tcggcatgcg cggctcgcac accggcgagc tcgtcttcca gaacgtcgag
    10441 gtgccggccg agaacgtgct cggcgcagaa ggcggcggcg cgaaggtgct gatgagcggg
    10501 ctcgatttcg agcgcgccgt gctctccggc ggaccgctcg gcatcatggc tgcctgcatg
    10561 gacgccgtgg tcccgtatgt gcatgaccgg cagcagttcg gtcagtcgat aggcgaattc
    10621 cagctgatgc agggcaagct cgcggacatg tacacgacaa tgatggcctg ccgcgcctac
    10681 gtctacgagg tcggccgcgc ctgcgacctt gccacgccgg agaccgtgcg ccatcttcgc
    10741 aaggatgccg ccggcgcgat cctgtattcg gccgaaaaag cgacctggat ggccggcgaa
    10801 gcgatccagg cgctcggcgg caatggctac atcaacgatt accccaccgg ccgcctgtgg
    10861 cgagacgcga agttgtatga aattggcgcc ggcacctcgg agatcaggag aatgctgatc
    10921 ggccgcgagc ttttcgcgga gacgatgtag gtcgccacca tgcatccctg gcaacagcac
    10981 ctcgatcaac tcagggccgg gttcaagccg ccgggcctgg ctgacgcaga tacgctgaag
    11041 cacatcagcg ggctcgaatt cctgcgccgg atgattcgcg gcgagatccc gactgcgccg
    11101 atctcgcaga cgctcgactt ctacctgatc gaggccgatc cgggccgtgc cgtgttccag
    11161 ggccggccca cgcatgcgca ctacaacccg attgccaccg tccacggtgg ctaccatgcc
    11221 acgctgctcg actccgcgat gggctgcgcg gtgcagacgc tgtgcgaggc gggacgcgcc
    11281 tatacgacgc tcgagttcaa gatccactgc gtccgcccac tgacagagct gaccggaccg
    11341 gtccgcgcgg aaggcaaggt gatctccagc gggcgccgca tggcgacggc tgaagggcga
    11401 ttggtcgatt ccgaaggccg gctttactcg cacggaacca ccacttgcat gctgttcgac
    11461 ctgcagtctt gagccgcccg acatgaacga cgtgcacgca ctcgacaact cggtcgagaa
    11521 atccggcgcc gtgcggcaat actggaccga cttctgcgtg gcaacccggc ttgaccccgc
    11581 gaccccgttc caggcctggt acttcggcga cacgcccgca ctggcgcacg aactcgccga
    11641 actggtgctg cacggaccca agcgggccac cgcgggactg gctgaattca atgaccagat
    11701 gccgcaggtc aagccgatgc ccgggggcta ctcggtgctg accgagcatg acggcacgcc
    11761 gcgcgcggtg atccgtacga cggtgctcga gcgtcgggcc ttcgccgacg tcgatgcctc
    11821 gttcgcctgg gacgaaggcg aaggtgatcg cacgctcgct gactggaagg acggccaccg
    11881 ccgttacttc acgcgtgagc tggccacgat cggccgcgac ttcgacgaga cgcaagaagt
    11941 cgatctcgaa cggttcgaac tgctctatcc gtttgatcgg gcgctgcatc cggtggactg
    12001 cgggccgcgc ctgctgccat gcctgctgcc gggtgcgctg gcctccagcg ctgcgctgca
    12061 gtcgcagtac tacgcgcgcg agcacggctt cggtgttttt tttgaggccg gccggatggc
    12121 ggacatcgcc gcattccttt cgcgctacga cgtgcagcgc gatggtgtct gggtcgccgt
    12181 cgacagcggc caggtcatag gcacgatcgt catcgacggc ggcgccagtg ccgaccggac
    12241 gcaggcccag ctgcgatggt tcatcatggc cgatacattg cggggcaaag gcatggggcg
    12301 gcgcctgatg caggccgcga tggggtttgc ggtggcgcgc tacgcgcgcg tgtggctcgg
    12361 taccttcgat gcgctgcatg cggcgcgcca tctgtacgaa ggtttcggtt ttcgcaaggt
    12421 gctcgaacgg ccctccaccg aatggggccc ggaagtgcgg gaacagcact gggagtggat
    12481 aaggtgacgg tgttcggact cgggctcgac aagctgacgt tgttcgcgct ggcagtcctg
    12541 ctgctggcgc tgacaccggg tcccgtgtgg atctacctga tctcgcgcac gctcactcag
    12601 ggccgccgcg ccggctactt ctcgctgatc ggcgtcatgg ccggagtgct ggtgcacctg
    12661 atggcggcag ccctcggcct gtcggcgctg ttgctggcgc tgcccgccgc cttcgatgcg
    12721 atcaagctgg ccggtgcagc ctacctgttg tggctggcct ggagcacgct gcgtggcagc
    12781 ggcttcagct tcacgccgca gccgctcgac ccggtaccgg accgcgtgct gttccggcag
    12841 tcactgaccg cgtcggtggt gaacccgaag gtggcggtgt tctacctgtc gctcttcccc
    12901 cagttcgtcg atccggccag cggcccggtg ctgctgcaaa gcctggcgct gggaatcgtt
    12961 cacgtcaccg tctcgaccct ggtcgacggt gcgctggtca ccgtcgcggg ggcgctgtcg
    13021 gtctggatcg cgcggcggcc cgggtggctg cgcatgcagc gctgggtgct gggaagcgcg
    13081 ttcggcgcgc tcgcggtctg gctcgcggca acgccgcggc attcacccca ctagaactcg
    13141 acacagcaaa aatgtccgcc gttgccgatg tggtcttcga ccgttcgatg ccttcgcctg
    13201 cgcgcattgc gcacgcgatc ctggacggct tcaaccggca ctaccgcctg tttcggtacg
    13261 tcgcccagca ggccaaatcg ttgtacgaga acggcaactg gcagacgatg cggacgcggg
    13321 cgcgcgaacg catcgacttt tacgaccagc gcgtgctcga ggctgtggcc cggatcgagc
    13381 acgaattcga ccttgcctcg ctcgaggatg ccgagcgcga cgcgctgtgg caggcggtca
    13441 agcagcagta cgtcacgctc ctcgccgagc accgccagcc ggagtgtgcg gagaccttct
    13501 tcaattcggt gtgcaccaag ctgttgcatc gcgagtacta ccgcaaccag ttcctgttcg
    13561 tgcggcccgg tgtggcgacc gactacatgg actcggaccc gccgtcgtat cgcagctatt
    13621 acccgtccgc gggactgcgc acgacgctgc gacagatcgt catcgacgcc ggactggcgt
    13681 gccgctggga ggatgtcgaa cgcgacctgc ggctgatcgt gcgtgcggtg tgcgaagcgc
    13741 atcgcagcgg tgaagcgttc cggcgcccct ggcacatgga gccggattgc cagatccaga
    13801 tcctgtcgtc gctctttttc cgcaacaagg gcgcctacat cgtcggtcgc gtgatcaacg
    13861 gcacgcgcat tacgccgctc gcggtaccga tcctgcgcaa ttcgcgcggg cagctttacc
    13921 tggacacggc gctcttcacc accgacctgc tggcgacact gttctcgttc acgcgcgcct
    13981 acttcctcgt cgacatggaa gcgccgtcgg cctacatcga cttcctgcgc accatattgc
    14041 cgcacaagcc gccgtcggaa ctgttcacga tggtcgggct cgccaagcag ggcaagaccc
    14101 ttttctatcg cgatttcctt catcacctga agcactcgac cgactccttc gtcatcgcgc
    14161 ccggcatcaa gggcctggtg atgtcggtgt tcaccctgcc gtcgtacccc tacgtgttca
    14221 aggtgatcaa ggacgtgatc ccgcatccga aggacaccga tcgccagcag atcatgggca
    14281 agtatcagct ggtgaagatg cacgatcgcg tcggccgcat ggccgacacc tgggaatact
    14341 cgcaggtgac gctgccgcgt gcacgatgct cgtcccagtt gatcgaggag cttcgcgcct
    14401 actgtccgag ccagctcgat ttcggcgacg gcacggtgac gatccggcat gtctatatcg
    14461 agcggcgcat gaccccgctc aacctgtatc tcgcaaaggc gtccgatgcc gacctcgacc
    14521 acgcgatgct ggaatacggc aacgcgatca aggaactggc ggccgccaat attttcccgg
    14581 gcgacatgct gtacaagaac ttcggcgtca cgcgcctgaa ccgggtcgtg ttctacgact
    14641 acgacgagat ctagtacatg actgactgca atttccgcac ggtgccgcag tcgcagtacg
    14701 acgacgacga gatgaacgcg gaggcctggt atcgcgtcga gaagcgcgac atgtttcccg
    14761 agcaatgggg cacgttcctc ctcggcgatg accgcatccg tgcggccttc ctgaagcacc
    14821 atgccgacct gctggcgcct gccttctgga acgcgcgcaa ggggcgcatc caggccgggc
    14881 acctggaaga cgtgttcccg tacccccccg agcgccgctt ccggcgtcgc tatgcgtcag
    14941 aattcgaagt tcactgagga gaagccatga acgatccgat cgtcattgtt tccgccgccc
    15001 gcacgccgat cggtggtttg ctgggtgact tcacgtccct gcaggccacc cagctcggcg
    15061 cgttcgcgat ccgcgccgcc gtggaccgcg ccgggatcgc gggcgacgcc gtcgatgaag
    15121 ccttgatggg ctgctgcctg atggccggcc agggtcaggc accggcgcgc caggcggtgc
    15181 taggcgccgg actgccgcag tcggtgggcg cggtcacgct gtcgaagatg tgcggctccg
    15241 gcatgcgcgc gatgatgttt gcgcacgaca tgctcgtggc cggaagcgct gacgttgtgc
    15301 tcgccggcgg catggagagc atgaccaacg cgccccacct gctggtcggc tcgcgccgcg
    15361 gctatcgcta cggcgcattc acgacgtacg accatatggc gctcgacggc ctggaagacg
    15421 cctacgaacg cggcaaggca atgggagtgt tcgccgagca gtgcgtcgca aagtacagtt
    15481 tcacgcgaga ggagcaggac gcctttgcga ttgcctcgac cgagcgcgcg ctgaaggcca
    15541 ataccgacgg cagctttgcc tgggagatcg cgccggtgac gatcgcgggc aagggcggtg
    15601 aggtcaagat cgacaaggac gagcagccgt tcaaggccaa gctcgacaag attccgggac
    15661 tgaagcccgc gttcaagaag gacggcacga tcaccgccgc caatgcgtcg tcgatttccg
    15721 acggtgcggc agcgctggtg ctgatgcgcc agtcgaccgc cgcgaaatcg gaccgcaagc
    15781 cgatcgcact catccgcgcg cacgccgttc atgcgcaggc gccggactgg ttcacgaccg
    15841 caccgatcgg cgcgatcaac aaggtgctgg agaaaaccgg ctggaagcct ggggacgtcg
    15901 acctctggga agtcaacgag gcctttgcag ccgtgacgat ggcggcgatg cgcgaactca
    15961 agctgccgca cgacaaggtc aacatccatg gcggcgcctg tgcactcggc catccgatcg
    16021 gcgcgtccgg cgcacgcatc gttgtcacct tgctcggcgc actccgcaag accggcggca
    16081 agcgcggcgt ggcggcgctg tgcatcggtg gcggggaagc gaccgcactg gcgctggaac
    16141 tgctgtgacg gcgcgcagtt gcgcgtcggc cctggccagc gaaatcagct gatgcacccg
    16201 cgcaagcgct ccaaccttcc gtccgattga cgatgcttgg cgtacaggac ctcggactct
    16261 tcgtcgtcac cgggctggtg ctcaatgcca cgccgggcgt cgacatggcg tacgccgtga
    16321 cgcggacgtt gcagtacggc ggtcggggcg gcgtcgcggc ggcgctcggc atcagtgccg
    16381 ggtgcaccgt gcacgccgtg gccgctgcgc tcggactggc ggcgttgctc gcagcctcgg
    16441 cctgggcgtt ctccgtgatc aagtgggccg gtgccctcta cctgttgtgg ctggcgtggg
    16501 gcatggcccg cgcggcatgg cggccgggcc agcccgccga ggccatcgcg ttgcctgcgt
    16561 cgacaccgcg ctcgcgcatc ttcgtccagg gtttcctgac caacgtgctc aatccgaagg
    16621 tggcgctgtt cttcatggca ctgctgccgc agttcatcgg cgcggatgcg ccgaacaagc
    16681 cgatggcctt cctgttcctc ggcgccgtgt tcatcttcaa cggcacgctc tttctgctgc
    16741 tgctcgtggt cctcgcgcag cggctgcggc gcgtgggcac gcggccagcg gtgcggcgcg
    16801 tcctgaacgg gctgggtgcc gcattgttcg cgggcctcgc gacccgcctg gcactggcgc
    16861 agcgctgaga tcgagcaatg gccaccgtcc tcatcatcgg cgcatcgcgt ggcatcgggc
    16921 tggagttcgt ccgccagtac gcggccgacg gtgcacgggt catcgccact gtgcgccggt
    16981 ccgaggacac cgcgaggttg cgcgagctgg gcgccaaacc gctgctgctc gatgtcgtcg
    17041 atgaggcgac ggtggccgag ttcggcgtgc agctgcatgg tgaaaagatc gacgtcgcga
    17101 tctacaacgc cggagtctat gggccgcgaa ccagtgccat tgccacgccc ggcggactcg
    17161 acttcgacgc cgtgatgcac acgaacgtgc gagcgccgat gcagctgatc ccgcagctgg
    17221 tgccggggct ggcggcggcc ggcggcaagc tcgcgctcat ctcaagccgc atgggttcaa
    17281 tctcgctgat gagcagcaca tcgggctggc tgtatcgtgc gagcaaggcg gcggccaacg
    17341 cggcgttgcg cgcgatgagc ctggaactcg gtccgcacgg catcgtgtgc atcgcgtttc
    17401 atccgggctg ggtgcgcacc gagatgggcg gcgccggcgc cgacatcgac gccgcgacca
    17461 gcgtgaccgg aatgcggcgc gtgctagcgt cggccaatgc atcgcagaac ggcaagttcc
    17521 tcaacttcaa tggcgaacag ttgtcctggt agatccatga acgacaagcg actgatctcc
    17581 tcgggttccg cgttcgaggc cgtggcgggc tacagccgtg ccgttgtcga gggcgattac
    17641 gtgcatgtcg cgggaacgac gggattcgac tacgcagcga tgacgatcga taccgacgcc
    17701 atggcgcaga cacaccagtg cttccgcaac atcgcttccg cgctcgagca ggccggctcg
    17761 tcactggacg atgtcgtgcg cgtgcgctac ttcctgacgg atgcggcgct cttcgagcag
    17821 cttgcaccgg tgttcgggaa gtacttcagt accgcgcgtc ccgctgcgac tgccatcgtc
    17881 tgcggcctgg tcgatgcgcg catgaagatc gagatcgaag tgaccgcacg tcgacgtgcc
    17941 tggagctgac gtgatgccat tgctccgcgc cgttcgcaac ccacaggacg catgctgaga
    18001 tgccactgca ggtcttcatc gtgctcgtat acggcatctg cagctggctg gcgattgcga
    18061 tcatcgaacc ggtcatgcgc agctttgcca acgaggccgg catggaaagt tggctgttca
    18121 tcgcgattcc gggcctcctg gcgatgctgt tcgccgtgct gtcgtacatc cgggcggcga
    18181 cgcgcgtcac caacatggag cagtccatgt cacgtgcgct gctggtggcg ctgctgacct
    18241 ggatcgccat cacgacgctg atcgcctggc tctggtgccc ggcacacagc gcgctgcgct
    18301 gcttttccaa tgccctgatc acgaccggca tcgttggcgg cgggccgctg ctcgtggggg
    18361 cgatggtggc cggcggcatc gtggcgctgg tgctgcgtca acgaccgtcg tggatctctt
    18421 ttggcgagcg tcgcgtgctc atggcgccgg acgccgaccc gaaccccacg tcgaagccgg
    18481 gcaatgctcg ctgaagagca tcttctgctg cgcgaggccc tgcgcgattt cgcttccaat
    18541 cgactcgccc cccatgccgc cgagtgggac cgcaacgcca cgttcccgaa ggaggcattg
    18601 aaggaactgg cggcgctcgg caccttcggc attgccgtgc cggaggaata cggcggcgcc
    18661 ggcatggact actccgcact ggcggtggcc ctcgaggaga ttgccgcagg cgacggagcg
    18721 acatcaacca tcatcagcgt caacaactgc cccgtgtgct cgatcctgat ggcctgggcg
    18781 aacgaggagc agaaacagcg ctacctggta ccgctcgcgc gcggcgagta cctgggcgcc
    18841 ttttgcctga ccgaaccgca tgtcggatcg gaagcctccg ggctgcgcac gaccgccgta
    18901 cgcgatggcg accactggat tctgaacggc gtgaagcagt tcatcaccag cggcaagcat
    18961 gccgatgttg cgatcgtgct ggcggtgacc gacaaggcgg ccggtaagcg cggcatcagc
    19021 gcattcatcg tcgagacctc gacgccgggt tacgtggtgg cacgcatcga ggagaagctc
    19081 gggcaacacg cctccgacac cgcgcagatc ctgttcgaga actgtcgtgt acccgcggcc
    19141 aacctgatcg gcgacgaggg cgcgggctac cgaatcgcgc tctccggcct cgagggcggg
    19201 cgcatcggca tcgctgcgca ctcgctcggc atggcgcgtg cggcctacga agccgcgttg
    19261 aagtatgccc gcgagcgcat cgcgttcgga caaccgatct tcgatcacca ggcggtgcag
    19321 tttcgactgg ccgacatggc cacacagctc gaggcggcgc ggcaactcat ccaccacgcc
    19381 gcagcgctga aggatgccgg gcagccgtgt ttgaagcagg ccgcgatggc gaagctcttt
    19441 gcgtcggaaa tggccgagcg ggtctgctcc gatgcgatcc agatccacgg cggctacggc
    19501 tacgtgagcg actttccggt cgaacggatc taccgtgatg tgcgcgtctg ccagatttac
    19561 gaaggcacca gcgacatcca gcgcatcctg atcgcgcgaa atttatagtc tctcgccggc
    19621 gcgtacgcat tcgctgcgcg aatgcgcgtg cgcttcggcg agttgattgg tgcggagtgc
    19681 ttggggcggc ccggcgcccg tcgcgtgcac aaatgcagga cagcccggcc cccgtacaaa
    19741 ttctgatgga gacagcatgc gcgtgatact ggcgggagca acgggatggg cgggctcggc
    19801 aatcgcgcgc ggaatcgcgc gagctgatga cctgcagctt gtagcgtcgg ttgcacgaaa
    19861 ggcggcggga caaaacctgg cggccgtcct cggtgagcct gcactgtccg ctccggtgct
    19921 tgcaactgcg gccgaggcgc tcgcgacgcc atgcgatgtc tacgtggagt tcaccaagcc
    19981 ggcaggagcc aaagcgaaca tcctcgccgc actggatgcg ggcgttcatg tggtggttgg
    20041 cacttccggg ttgagcgacg tcgactacgc cgaaatcgat ggggtcgcgc gcgcgcgggg
    20101 acgcggcgtg ctggcctgtg gcaacttcgc gctcacggtc gtgctgcttc agcgtttcgc
    20161 ggagatcgcg gcgcgctatc tcgatcactt cgagatcatc gactacgccc atgaagacaa
    20221 gccggacgca ccgtccggca cggcacgcga actggcgttt cggcttggcc aggtcaaggc
    20281 gccggacccc ggcgtgccgc tcgaccaggt gcgcggaccg cgagaggcgc gcggcgcgac
    20341 gctcaatggc gtgcaggtcc actcggtgcg cctgcccggc tacgtgat
//
LOCUS       DATIRT010000474         4938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_672347, whole genome shotgun sequence.
ACCESSION   DATIRT010000474 DATIRT010000000
VERSION     DATIRT010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672347"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VLN25_10880"
     CDS             complement(<1..365)
                     /locus_tag="VLN25_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HSQ11096.1"
                     /translation="MTPTADLSILSLITKATVVVQFVMLLLAVLSIASWTAIFQKGFA
                     IKRAKRETEEFERQFWGGAELSQLYQQASNPRRKSGTLERIFEADMSEFLKLRGRGDA
                     QATLDGARRAMRASYQREM"
     gene            complement(362..811)
                     /gene="ybgC"
                     /locus_tag="VLN25_10885"
     CDS             complement(362..811)
                     /gene="ybgC"
                     /locus_tag="VLN25_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HSQ11097.1"
                     /translation="MSTTLPSATGQPFTWKIRVYYEDTDTGGIVYYANYLKFFERART
                     EWLRAAGYEQRRLSEEFGLQFVVARVQCDYRQPARLDDLLEIDLRVLKSGRVFIVFEQ
                     TARRGATVLATATVKAGCIDTHRLVPAAMPAELLDAFRTLPGSLATS"
     gene            991..3249
                     /locus_tag="VLN25_10890"
     CDS             991..3249
                     /locus_tag="VLN25_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSQ11098.1"
                     /translation="MTMGCSRLAALSRAGVLLLAGAAIFTGCDRVTNSPHPNGAEKTN
                     TFFTAFTERSPKYLDPTASYSLDETPYTYAIYEPLYRFHYLKRPYEVAPRAAEAIATP
                     RYYDKAGRELPADAPGAEIAESVYDIRLTRGILFSPHPAFAKDDAGNFRYHAMTAKDL
                     GDKRTPFDFEHRDTRELTAHDYVYAIRRLATTRIKSPSFSPMAEHIVGLKDYGELIAR
                     EDEKLRSGKPLTDRNLPFLDFRQFPLAGVEALDDHTLRIRLKGKYPQFKYWLTMSFFS
                     PVPWEVDKFYAQPGMNERNLSMNYWPVGTGPFMLTEYQENRRHVLSRNPNFRGEPYPC
                     VGEEADKAAGLLADCGKPMPFVDQVVFSIEKERIPHKTKFMQGYYDNPDVSRFDWFQD
                     LDYDVKNSEKVAQLFRERGIQMPKLLEISNWYLGFNWWDPVVGKGKTPEEQERNRKLR
                     QALSIAIDWEEFVRVFESKAAGEPAMGAVPPGVFAYRADAINRSVYDIVDGKPRRKSI
                     EAAKKLLADAGYPDGRDAKTGQPLVLNYDYQRALTPELKAEVEWMVKQFAKLGVQLEL
                     RATDYNRFQDKADKGSLQIFWWGWFADYPDAENFLFLLYGPNSQAATQGNGNNTANYQ
                     NAEFDKLFEEMRYLDDVPRKQQVVDQMIAILQKDAPWAFGYNPYAGSVHHGWVGNVKP
                     APLANDRLMYFKVNPEQRAAKIAEWNRPIWWPVLLIVLVLVAAVVPAFRSWKRRERET
                     AGRTLATPKAAE"
     gene            3278..4255
                     /locus_tag="VLN25_10895"
     CDS             3278..4255
                     /locus_tag="VLN25_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSQ11099.1"
                     /translation="MLNYIVRRISYAALILIGVNLLTFTLFFAVNSPDNIARLNIGAK
                     RVSQDAIEKWKAERGYDKPLWFNAKAEGTARITQTIFYDRSLRLFSLDFGTSDSGRDI
                     RHEIATRIPASLALAGPMLLLTLFSGIAFSLLLVFFRASYLDFWGTVVCVVMMSISSL
                     FYIIMAQWFFGKTLKLVPLSGFVPGLDLVKFLALPLVVGIVFQLGSEARLFRAMLLEE
                     IGKDYVRTARSKGLAESAVLFGHVLRNAWLPILTHVGALLPYAFLGSIVFESFFGIPG
                     LGTYVVEAIAGQDFAIVHTMVFLGSLLYILAYLATDIAYTFVDPRVRLA"
     gene            4538..>4938
                     /locus_tag="VLN25_10900"
     CDS             4538..>4938
                     /locus_tag="VLN25_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSQ11100.1"
                     /translation="MPKFVFLWTDLVLWLVVAIVIGYVVHVRRSPNLRATWRHVLRSA
                     PTMSAAVVLIVFIAIALLDSIHFRPRLSAAPGAAPDIQQAYATRTLSPLDVLLRHAVD
                     AREKTYSVPLAYWSFQRETAVIDGKEVRELPR"
BASE COUNT          914 a         1636 c         1515 g          873 t
ORIGIN      
        1 tccatctcgc gctggtacga cgcgcgcatg gcccgccgcg cgccgtcgag tgtggcctgc
       61 gcatcaccgc ggccgcgcag cttcaggaac tcgctcatat ccgcctcgaa gatccgctcc
      121 agagtgccag acttgcgccg cggattcgag gcctgctgat acagttggct gagctcggca
      181 ccaccccaga actggcgctc gaattcctcg gtctcgcgct tcgcccgctt gatcgcaaaa
      241 cctttctgga agatcgccgt ccacgatgca atcgacagta cggcaagcag cagcatcacg
      301 aactgcacga ccaccgtggc cttggtgatg agcgagagaa tcgaaaggtc ggcggtaggt
      361 gtcatgaggt ggcaagtgat ccgggcaagg tacggaatgc gtcgagcaat tcggcaggca
      421 tcgctgccgg gacgaggcgg tgcgtgtcga tgcagcccgc cttgacggtc gccgtggcca
      481 gcacggtcgc gccgcgccgg gccgtctgct cgaacacgat gaatacacgg cccgacttca
      541 ggacgcgcag atcgatctcg agcaggtcat ccagtcgggc cggctgccga tagtcacact
      601 gcacacgcgc cacgacaaat tgcaggccga actcctccga caaacggcgc tgctcgtagc
      661 ccgccgcgcg caaccactcg gtgcgcgcgc gttcaaagaa cttcaggtag ttggcgtaat
      721 agacaattcc gccggtatcc gtgtcctcgt aatagacgcg gatcttccac gtgaagggct
      781 ggccggtcgc agagggaaga gtcgtcgaca tgcgctgcac cgcagtataa gggcgtaagt
      841 cgcccgttcc gggcacgacc ggtgcagcgt ctagagacgg ccgtcgcgct tcatcctatc
      901 ctggccaact acaatgcgcg ccttttgccg gtcccacgat gaccgagcaa gctgcccggc
      961 aacacggccg cgaccacgcg gggaggacag atgaccatgg ggtgcagcag gctcgcggcc
     1021 ttgagccgcg ccggtgtcct gctgctcgcg ggtgcggcca ttttcaccgg atgcgaccgc
     1081 gtcaccaaca gcccgcatcc caacggcgcg gagaagacca acaccttctt caccgcgttc
     1141 acggagcgtt cgcccaagta cctcgacccg accgcctcgt actcgctcga cgagaccccg
     1201 tacacctacg cgatctacga gccactgtac cgatttcatt acctgaagcg gccgtacgaa
     1261 gtggcgccac gcgccgcgga ggcgatcgcc acaccgcgct actacgacaa ggcgggccgc
     1321 gagcttccgg ccgatgcgcc cggcgccgag atcgccgagt cggtctacga catcaggctg
     1381 acgagaggca tcctgttctc tccgcacccg gcctttgcga aggacgatgc gggcaacttc
     1441 cgatatcacg cgatgaccgc gaaagatctc ggtgacaaac gaacgccctt cgatttcgag
     1501 caccgcgaca cgcgcgagct gacggcgcat gactatgtgt acgcgattcg gcgcctggcg
     1561 acgactcgta tcaagtcgcc ttcgttctcg ccgatggccg agcacatcgt cgggctcaag
     1621 gactacggcg agctcatcgc cagggaagac gagaagctgc gcagcggcaa gccgctgaca
     1681 gatcgcaacc tgcctttcct cgacttccgc cagtttccgc tggccggcgt cgaggcgctc
     1741 gacgatcaca cgctcagaat ccgcctcaag ggcaagtacc cgcagttcaa gtactggctg
     1801 acgatgagct tcttttcccc ggtgccgtgg gaggtcgaca agttctatgc ccagcccggc
     1861 atgaacgaac gtaacctcag catgaactat tggccggtcg gcaccggccc cttcatgctc
     1921 accgaatacc aggaaaaccg ccgccacgtg ctctcgcgca atccgaactt ccgcggcgag
     1981 ccgtatccgt gcgtaggcga ggaagcggac aaggcggctg gcctgctcgc cgactgcggc
     2041 aagccgatgc ccttcgtcga ccaggtcgtc ttctcgatcg agaaggagcg gatcccgcac
     2101 aagaccaagt tcatgcaggg ctactacgac aacccggatg tctcgcgttt cgactggttc
     2161 caggacctcg actacgacgt caagaactcc gagaaagtgg cgcagctctt ccgcgagcgc
     2221 ggcatccaga tgccgaagtt gctcgagatc tccaactggt acctcggctt caactggtgg
     2281 gacccggtcg tcggcaaggg caagacgccg gaggaacagg aacgcaaccg caagctgcgc
     2341 caggcgctgt caatcgccat cgactgggaa gagttcgtcc gcgtgttcga aagcaaggcc
     2401 gccggcgagc cggcgatggg cgccgtgccg ccgggcgtct ttgcgtaccg tgccgacgcc
     2461 atcaaccgca gcgtgtacga catcgtcgat ggaaagccaa ggcgcaagtc gatcgaggca
     2521 gcgaagaagt tgctggccga tgccggctac ccggacgggc gcgatgcaaa gaccggccag
     2581 ccgctggtac tgaactatga ctaccagcgc gcgctgacac ccgagctcaa ggccgaggtc
     2641 gagtggatgg tcaagcagtt cgccaagctg ggcgtgcagc tcgaactgcg agccaccgac
     2701 tacaaccgct tccaggacaa ggccgacaag ggcagcctgc agatcttctg gtggggctgg
     2761 tttgccgact acccggacgc cgagaacttc ctgttcctgc tgtacggtcc caactcgcag
     2821 gccgccacgc agggcaacgg caacaacacg gccaattacc agaacgccga gttcgacaag
     2881 ctgttcgagg agatgcgcta cctcgatgac gttccgcgca agcagcaggt tgtcgaccag
     2941 atgatcgcca tcctgcagaa ggatgcaccg tgggcgttcg gatacaaccc atatgcgggc
     3001 tcggtgcatc acgggtgggt cggcaacgtg aagccggcac cgctggcgaa cgatcgcctc
     3061 atgtacttca aggtcaaccc cgaacagcgt gccgcgaaga tcgccgaatg gaaccggccg
     3121 atctggtggc cggtcttgct gattgtgctg gttcttgtcg ccgccgtggt tccggcgttc
     3181 cgcagctgga agcggcgcga gcgcgagacc gcgggacgga cgctggcgac cccgaaggcg
     3241 gccgagtgac ggctgcgacc gcgcgtcgaa agcattcgtg ctgaactaca ttgtccggcg
     3301 catcagctac gccgcgctga tcctgatagg cgtgaacctc ctcaccttca cgctgttctt
     3361 tgcggtcaac tcccccgaca acattgcgcg cctgaacatc ggcgccaagc gcgtgtcgca
     3421 ggatgcgatc gagaagtgga aggccgagcg gggttacgac aagccgttgt ggttcaacgc
     3481 caaggccgaa ggcaccgcca ggatcacgca gacgatcttc tacgaccgct cgctgcgcct
     3541 gttcagcctc gacttcggca ccagcgacag cgggcgcgac atccgccacg agatcgccac
     3601 ccgcattccc gccagcctgg cactggccgg accgatgctg ctgctgacgc tgttctctgg
     3661 catcgcgttc tcgctgctgc tggtgttctt ccgcgccagc tacctcgact tctggggcac
     3721 cgtggtgtgc gtggtgatga tgtcgatctc gagcctgttc tacatcatca tggcccagtg
     3781 gttcttcggc aagacgctga agctggtgcc attgtcgggc ttcgtgccgg gactcgacct
     3841 ggtgaagttc ctagctctgc cgctcgtcgt cggcatcgtg ttccagctcg ggtccgaggc
     3901 gcgcctcttc cgcgcgatgc tgcttgagga aataggcaag gactatgtgc gcaccgcgcg
     3961 ctccaaagga ctggccgagt ccgcggtgct gttcggccat gtgttgcgca acgcctggct
     4021 gccgatcctg acgcatgtcg gggcgctgct gccctacgcg ttcctcggtt cgatcgtgtt
     4081 cgagtcgttc ttcggcatcc cgggcctggg aacctacgtg gtcgaggcga tcgccggcca
     4141 ggacttcgcc atcgtgcaca ccatggtctt cctcggctcg ctgctgtaca tcctcgcgta
     4201 cctcgcgacc gacatcgcct acacgttcgt cgatccccgc gtccggttgg cctagaacct
     4261 atctcagagt ccccgatgga tcgcgttgcc aagagaagcg ggcggatgca aggcggctgg
     4321 cgaaggacag aagccggtcc tacggccgag ccggccaacg ccgcagacac ccgcttgttt
     4381 tggcaaccct ccggggcggg gtcgtgcatt gacaacgtca gggcgcaggg cgttgttacg
     4441 gctcgggtcc ttgcacgagc aatggtcccc tcgccgcgcc tagccctgca cccaatgcga
     4501 ccccgccgcg accaccgagg actctgagat aggttctatg ccaaagtttg tcttcctctg
     4561 gaccgacctc gtactttggc tcgtggtcgc gatcgtcatc ggctacgtcg tgcatgtgcg
     4621 tcgcagtccc aacctgcgtg ccacctggcg tcatgtgcta cgcagcgcac cgaccatgtc
     4681 ggcagcggtc gtgctgatcg tgttcatcgc gatcgcgctg ctcgactcga tccacttccg
     4741 gccgcggctg tccgctgcac ccggcgccgc gccggatatc cagcaggcct atgcgacgcg
     4801 cacgctgtca ccgctcgacg tgctgttgcg ccacgcggtc gatgcccgcg agaagaccta
     4861 ctcggtgcca ctcgcctact ggtctttcca gcgcgaaacc gccgtaatcg acggcaagga
     4921 agtccgtgag ctgccgcg
//
LOCUS       DATIRT010000475         9839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_967090, whole genome shotgun sequence.
ACCESSION   DATIRT010000475 DATIRT010000000
VERSION     DATIRT010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9839
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967090"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..1275)
                     /gene="tgt"
                     /locus_tag="VLN25_10905"
     CDS             complement(133..1275)
                     /gene="tgt"
                     /locus_tag="VLN25_10905"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HSQ11101.1"
                     /translation="MSSGFELVASDGAARRGRLTLAHGTVETPVFMPVGTYGTVKAMA
                     PTELVEAGAQIVLGNTFHLWLRPGLEVIGAHGGLHRFMGWSGPILTDSGGFQVFSLGP
                     LRKIAEDGVRFASPVNGDRLFLTPEESMRIQAVLNSDIAMVFDECTPYEIDGRPATLD
                     EAAVSMRLSLRWAQRSRDEFQRSGEAKGHRNALFGIVQGGMYETLRDESLDGLVDIGF
                     DGYAIGGLSVGEPKEDMLRVLSHTTPRLPSDRPRYLMGVGTPEDLLDGIAAGIDMFDC
                     VMPTRNARNGWLFTRHGDLKLRNARYRDDLGPVDPTCQCPTCRQFSRSYLHHLQQVNE
                     ILGARLNTVHNLHFYLGLMREARTAIEAGRYEAFRRQFAGDRARGV"
     gene            complement(1272..2330)
                     /gene="queA"
                     /locus_tag="VLN25_10910"
     CDS             complement(1272..2330)
                     /gene="queA"
                     /locus_tag="VLN25_10910"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HSQ11102.1"
                     /translation="MAGQAIAERELTLANFDFDLPPELIAQHPLADRSASRLLHVTPP
                     ALHDLHFTDLERLLGPDDMLVFNDTRVIKARLLGRKPSGGKVEALVERVVEPSLALAL
                     VRTSHRPAIGATFIFDETVLATVEGRKDDFFVLRFDGDVLSILDRHGHVPLPPYISHT
                     DSPLDAERYQTVYAKRPGAVAAPTAGLHFTPALLERLHAKGVQSTRLTLHVGAGTFHP
                     VRTERLDDHRMHDEWYDVPRAAAAAINDARQSGRRIVAVGTTSLRALESAAIDADHVR
                     ASAGETALFIRPGFRFRVVDRLITNFHLPRSTLLMLVSAFAGSDHIRSAYAHAITSRY
                     RFFSYGDAMLLERASRTP"
     gene            complement(2560..2646)
                     /locus_tag="VLN25_10915"
     tRNA            complement(2560..2646)
                     /locus_tag="VLN25_10915"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2610..2612),aa:Leu,seq:caa)
     gene            complement(2694..3155)
                     /locus_tag="VLN25_10920"
     CDS             complement(2694..3155)
                     /locus_tag="VLN25_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HSQ11103.1"
                     /translation="MANDSNGLAGAEYVGFWARVGAALIDTTLLLLIITPLLYWIYGP
                     EYFVGSDAFGGAADLLLNWVLPAVAVIVFWIYRQATPGKIAIGARIVDARTGGRPSTG
                     QLIGRYFGYYVSMIPLGLGLIWVAFDARKQGWHDKLAGTVVIKDRPTSTAN"
     gene            complement(3155..3997)
                     /gene="panC"
                     /locus_tag="VLN25_10925"
     CDS             complement(3155..3997)
                     /gene="panC"
                     /locus_tag="VLN25_10925"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HSQ11104.1"
                     /translation="MKVVHTIEALRDQLRGQNRIAFVPTMGNLHAAHLSLMAMAHQHG
                     DPVVASIFVNRLQFGPSEDFDKYPRTLQDDIDKLQQNNHVYVLFAPNESEMYPEPQSY
                     RVQPPHDLGDILEGEFRPGFFIGVSTVVLKLFSCVQPRVAVFGKKDYQQLMIVRNMCR
                     QFALPTEIIPHETVRDPDGLALSSRNRFLSETERKEAPRLYATLNSVRDRVRSGEHDV
                     ARVEHDAERELAANGWKVDYVSVRRQRDLNAPATGEVQSGEPLVVLTAARLGATRLID
                     NLEI"
     gene            complement(4144..4974)
                     /gene="panB"
                     /locus_tag="VLN25_10930"
     CDS             complement(4144..4974)
                     /gene="panB"
                     /locus_tag="VLN25_10930"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HSQ11105.1"
                     /translation="MSTHPQPPPTAARKPVTLPRLREMRAASEPIAMLTCYDASFARL
                     LDAQGVDCVLVGDSLGMVIQGLDSTLPVSMDEVAYHTRCVARGLSSAWLIADMPFGSY
                     QGGPEQALDSAVELMQAGAKMVKLEGGEWLAPTVAFLVERGIPVCAHLGLTPQSVHAL
                     GGYRIQGRSESDAERLLADAKALDDAGAALMVLELIPSALASRVTDAVALATIGIGAG
                     SQVSGQVLVLHDMLDVYPGRKPRFVRNFMQGQPDIASAVAAYVAAVKDRSFPSAEHGY
                     "
     gene            complement(5030..5671)
                     /locus_tag="VLN25_10935"
     CDS             complement(5030..5671)
                     /locus_tag="VLN25_10935"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HSQ11106.1"
                     /translation="MIAGRYRYVAIEGPIGAGKTSLTRRLAQHFNAHTLLERPEANPF
                     LERFYGDRARFALPTQLFFLFQRVQQLADVRQLDMFRAVLFADFLLDKDPLFARLTLA
                     DDELKLYEQILAQLRPQTPAPDLVIYLQASPETLADRVVRRGNPAEARISEEYLAALA
                     NSYTRFFHQYDEAPVLIVNTEHLNPIDSDADFHLLLDRIGKLRGRREYFNLAA"
     gene            complement(5742..6239)
                     /gene="folK"
                     /locus_tag="VLN25_10940"
     CDS             complement(5742..6239)
                     /gene="folK"
                     /locus_tag="VLN25_10940"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HSQ11107.1"
                     /translation="MKRIAYIGLGSNQGDVVEALNSAIESLRSIEQTEFVAVSPYYRS
                     APIDATGPDYLNAVVKVETSLEPYALLLHLLDIELMLGRKRRGLKKNAPRNVDLDLLL
                     LGDMIIQSTPLTLPHPRLHLRAFVLRPLVDLAPDIRIPGQGPASAFLDSVDDQKIEPV
                     EAAAP"
     gene            complement(6262..7644)
                     /gene="pcnB"
                     /locus_tag="VLN25_10945"
     CDS             complement(6262..7644)
                     /gene="pcnB"
                     /locus_tag="VLN25_10945"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HSQ11108.1"
                     /translation="MIRRVIARVSSLFSRKPRKPREPKRVPAAEHGIDQDLVARSALR
                     VCETLQKAGHRAYLVGGAVRDLLLGVAPKDFDIATDATPEQVKSHFRRAIIIGRRFRL
                     VHVMFGQETIEVSTFRALDNPDRKTDEHGRVLADNLFGEQHEDATRRDFTVNALYYDP
                     TTREVIDYHDGVKDVRRKRLRMIGDPETRYREDPVRMLRAVRFAAKLGFDIDEATREP
                     IPRLARLIENVPTARLFDEMLKLLTSGHSVACITRLRMEGLHHGLLPLLDVILEQPAG
                     ERFVLQALARTDERVLGGKSISPGFLFATLLWHDVLVKWNARTAQGEHRIPALNAAID
                     DTLDAQTEKLSVQRRFVSDMREIWGLQPRFERRSGRSAFRLFEHLRFRAGYDFLLLRA
                     AADEIPAELADWWTRFSHANEEERERLVEDAARSGVTEAPGRKRRRRTRGKGGASHAA
                     TDTSSETGMA"
     gene            complement(7675..8349)
                     /locus_tag="VLN25_10950"
     CDS             complement(7675..8349)
                     /locus_tag="VLN25_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HSQ11109.1"
                     /translation="MHLALFDLDHTLLPIDSADTWSHHVVERGGLDSADYGARIRRFA
                     DEYRAGTFDVDAYVAFQMELLAAFPRATLDDWHREFMQRHVSPAIREQARALVERHRA
                     AGDELALVTGTNAYVVTPIARSFGIDHVLAVEPEQDNGRFTGGHVGTHTYQGGKVRKV
                     EEWLAARGHTLADTITTFYSDSINDLPLLEKVTHPVVTNADARLLAIARERGWRTLQL
                     FDPAHA"
     gene            complement(8392..9078)
                     /gene="hda"
                     /locus_tag="VLN25_10955"
     CDS             complement(8392..9078)
                     /gene="hda"
                     /locus_tag="VLN25_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006682698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HSQ11110.1"
                     /translation="MSSPYRNSPHQQLALDLIQPLRPSLDNFVVGRNAEVIAALRTLA
                     AGGAERFVYLWGGEGSGRTHLLQAVAASASSRWLDSASSTLEFDPAVQLYLADDVDRY
                     DPMRQQQLFVLQNEIRASRDSGLVASGSAPPSQLVLREDVRTRLAWGLVYQLHGLTDA
                     EKEDALRAHAASRGIDLSADVLGYLLTHMPRDLSTQVAILNALDAYALEAKRAITVPL
                     VREWAAQADA"
     gene            9194..>9839
                     /locus_tag="VLN25_10960"
     CDS             9194..>9839
                     /locus_tag="VLN25_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HSQ11111.1"
                     /translation="MNPLDYKQAGVDYASIDPLKILAQQAAAGTAGNLARHGLIEVAA
                     SRGESAYVVDCGDFYLASITECLGTKALVADAMRAITGRTYYDQIAQDTLAMALNDII
                     TVGATPVSVHAYWATGGSDWFADAARARDLVDGWRAACDRSGVAWGGGETPALAGVVE
                     SGRIDLAASCVGLVRPKSRLTLGERLGDGDAIVLLASSGIHANGVSVARKLAERL"
BASE COUNT         1662 a         3139 c         3343 g         1695 t
ORIGIN      
        1 agggaacccc gcaggggcgc cttcgcaggc gcggctttct ttggttactt tctttgccaa
       61 gacaaagaaa gtaacgggcc gcgcggccct gaagcgcgcc ttggaaggca ggcaggtccc
      121 gccgcgcagc gactacaccc cccgtgcccg atcccccgcg aactgcctgc ggaacgcctc
      181 gtatcgaccc gcttcgatgg cagtacgcgc ctcgcgcatc agcccaaggt agaagtgcag
      241 gttgtgcacg gtattgagcc gcgctccgag gatttcgttg acctgctgca gatggtgcag
      301 gtagctgcgg ctgaactgtc ggcaggtcgg gcactggcag gtcgggtcga ccggacccag
      361 atcgtcgcga taacgggcat tgcgcagctt caggtcgccg tgccgggtga aaagccaccc
      421 gttgcgcgca ttgcgggtgg gcatcacgca gtcgaacatg tcgatgccgg ccgcgatgcc
      481 gtcgagcaga tcctccgggg tcccgactcc catcaggtaa cggggccgat cgcttggcag
      541 gcgaggcgtt gtatggctca gcacgcgcag catgtcctcc ttgggttcgc cgaccgacag
      601 gccaccaatc gcatagccgt cgaaaccgat atcgacgagc ccgtctagcg attcgtcgcg
      661 caaggtctcg tacatgccac cctgcacgat gccgaagagc gcattgcgat gaccctttgc
      721 ttcgccagag cgttgaaact cgtcgcggct gcgctgcgcc cagcgcaacg aaagacgcat
      781 tgaaacggcc gcttcgtcca gcgtggccgg ccgcccgtcg atctcgtacg gcgtgcattc
      841 gtcgaacacc attgcgatgt cggaattgag cacggcctgg atgcgcatcg attcctccgg
      901 tgtcaggaac aaccggtcgc cgttgacggg cgaggcgaag cgcacgccgt cctcggcgat
      961 cttgcgcagc gggccgagcg aaaagacctg gaaaccgccg gaatcggtca ggatcgggcc
     1021 gctccagccc atgaagcgat gcaggccacc gtgcgccccg atcacctcga gtccggggcg
     1081 tagccacagg tgaaaggtgt tgcccagcac gatctgcgcg cccgcttcaa cgagctcggt
     1141 cggtgccatc gccttgaccg tgccgtaggt gcccaccggc atgaagaccg gcgtttccac
     1201 cgtgccgtgc gccagcgtca ggcggccgcg gcgcgcggcg ccatcgctgg cgacgagttc
     1261 gaagccgctg ctcatggcgt ccgcgaggcc cgctcgagca acatcgcatc gccgtagctg
     1321 aagaaacggt aacgggaggt gattgcgtgt gcgtaggccg aacgaatgtg gtccgagcct
     1381 gcgaacgcgg ataccagcat cagcagcgtc gagcgtggta ggtggaagtt ggtgatcagc
     1441 cgatcgacga cacggaatcg gaagccgggc cggatgaaaa gcgcggtttc gcccgcactg
     1501 gccctgacgt gatcggcgtc gatcgcggcg gactcgagcg cgcgcaggct ggtcgtgccg
     1561 accgcgacga tgcgcctgcc gctctgtcgc gcatcgttga tcgccgccgc agccgccctc
     1621 ggcacgtcgt accactcgtc gtgcattcga tgatcgtcca gccgctcggt ccgcaccggg
     1681 tggaacgtgc cggcgccgac gtgcagcgtc agccgggtcg actggacccc ctttgcatgc
     1741 agccgttcga gcagcgccgg cgtgaaatgc agtccggccg tcggcgcggc caccgcgccc
     1801 gggcgcttgg cgtagacggt ctggtagcgc tcggcatcca acggactgtc ggtgtgggaa
     1861 atgtacggcg gcagcggcac atggccgtga cgatcgagga tcgacagcac gtcgccatcg
     1921 aatcgcagca cgaagaaatc gtcttttcgc ccttcgacgg tcgccaggac ggtctcgtcg
     1981 aagataaagg tggcaccgat ggcgggtcgg tggctggtac gtaccagtgc cagcgccagc
     2041 gagggctcca ccacacgttc gaccagcgcc tcgacctttc caccgctcgg cttgcgtccc
     2101 agcagccgcg ccttgatcac gcgggtgtcg ttgaagacga gcatgtcgtc cggtccgagc
     2161 agccgctcga ggtcggtgaa gtgcaggtcg tgcaacgccg gcggcgtgac atgcagaagg
     2221 cggctcgcgg agcgatcggc cagcgggtgc tgtgcaatca gctccggtgg cagatcgaag
     2281 tcgaaattgg cgagcgtcag ctcacgttcg gcgatggctt gaccggccat gcgtgcctca
     2341 ttgcgccaac ggcgcataga acacatctcg ctgaacacgc cttcgttcag cttggtgccg
     2401 ggaaagggaa tcgaaccctt acggtattgc taccgacctc tgcaaatgct ccggctttgc
     2461 cttcgcagtt ttgtttccgg ccgcttgcta cgcaagctta acctcgcttc gcgaggttag
     2521 cctgcaacga aatcccgcgg aacacgcctt cgttcagctt ggtgccggga aagggaatcg
     2581 aacccttacg gtattgctac cgcgggattt tgagtcccgt gcgtctacca gttccgccat
     2641 cccggctggg cggcgaatta tctatgaaag ggcggggccg accaaatccg cgttcagttc
     2701 gcggtcgacg tcggtcgatc cttgatgacg acggtaccgg cgagcttgtc gtgccagccc
     2761 tgcttgcggg cgtcgaaggc cacccagatc agtcccaggc ccagcgggat catcgagacg
     2821 tagtacccga agtagcggcc gatcagctgg ccggtcgatg gccgcccgcc ggtgcgcgcg
     2881 tcgacgatgc gggcaccgat tgcgatcttg cccggcgtgg cctggcggta gatccagaag
     2941 acaatgacgg ccactgccgg cagcacccag ttgagcagaa ggtcggcggc cccgccgaac
     3001 gcatccgatc ccacgaagta ctcggggccg tagatccagt acagcagtgg cgtgatgatc
     3061 agcaacaaca gggtcgtgtc gatcagagcg gcgccgacgc gcgcccagaa gccgacgtac
     3121 tcggcaccgg cgaggccgtt cgagtcgttg gccatcagat ttccaggttg tcgatcaggc
     3181 gcgtcgcgcc cagcctggcg gccgtcagta ccaccagcgg ttcgccggac tggacttcgc
     3241 ccgtggcagg ggcattcagg tcgcgttgac gtcgtaccga aacgtaatcg accttccagc
     3301 cgttggccgc cagttcgcgc tcggcgtcgt gctcgactcg tgccacgtcg tgttcgccac
     3361 tgcgcacgcg gtcgcgcacg gaattcagtg ttgcgtacaa gcgcggcgcc tccttgcgtt
     3421 cggtctcgct caggaatcgg ttgcgtgacg acagggccag gccgtcggga tcgcgcacgg
     3481 tctcgtgcgg aatgatctca gtcggcagcg cgaactgccg gcacatgttg cgcacgatca
     3541 tcagctgctg gtagtccttc ttgccgaaca cggccacgcg cggctgcacg caggagaaga
     3601 gtttcagcac gacggtcgat acgccgatga agaaaccggg tcggaactcg ccttcgagaa
     3661 tgtcgccgag gtcgtgcggc ggctgcacac ggtaactctg cggttccgga tacatctccg
     3721 attcattcgg cgcgaacagc acgtagacgt ggttgttctg ctgcagcttg tcgatgtcgt
     3781 cctgcagcgt gcgcgggtac ttgtcgaagt cctcgctcgg cccgaactgc agccggttga
     3841 cgaagatcga tgccaccacc ggatcgccgt gctggtgcgc catcgccatc agcgacaggt
     3901 gtgcggcatg gaggttgccc atggtcggga cgaacgcgat gcggttttgc ccgcgcagct
     3961 ggtcgcgcag cgcttcgatc gtatgaacga ctttcatcgt gtcggagccc gttgatcggg
     4021 cgcgcggtgg gcaaggccgc ggattgtaga gcgtggctcg gtgtcaggtg ctgccggtcg
     4081 gcgccggggc aagccgaccg gcgcgggagc ggtcaacgat ccagggccga ggtctggtgt
     4141 gtctcaatag ccgtgttctg ccgacgggaa gctacggtcc ttgacggcgg ctacgtacgc
     4201 tgcgacagcc gaggcgatgt cgggctgccc ctgcatgaag ttgcgcacga agcgcggctt
     4261 gcggccgggg tagacgtcga gcatgtcgtg cagcaccaac acctggcccg agacctggct
     4321 gccggcgccg atgccgatcg tcgccagcgc cacggcgtcc gtcacgcgtg atgccagcgc
     4381 gctcggaatc agttccagca ccatcagtgc ggcgcccgca tcatcgagcg ctttcgcatc
     4441 ggccagcagc cgttcggcgt ctgattcgct gcgcccctgg atgcgataac cgccgagcgc
     4501 atgcaccgac tggggcgtca gccccaggtg cgcacagacc ggaatgccgc gttcgaccag
     4561 gaaggcgacc gtgggcgcga gccactcgcc gccctcgagc ttgaccatct tggcgccggc
     4621 ctgcatcagc tcgacggcgc tgtccagcgc ctgctcggga ccgccctgat aactgccgaa
     4681 gggcatgtcg gcgatcagcc acgcggacga aaggccacgc gcgacgcagc gcgtgtgata
     4741 cgccacttcg tccatcgaga ccggcaatgt cgagtcgagg ccctggatca ccatgccgag
     4801 cgaatcgccg accagcacgc agtccacgcc ctgggcatcg agcagccgcg caaagctggc
     4861 gtcgtagcag gtcagcattg cgattggctc gctcgcggcg cgcatctcgc gcagccgcgg
     4921 caacgtgacc ggtttgcggg cagcggtggg agggggctgc ggatgggtgc tcatgcgaaa
     4981 actccgtatt gcgctctagg gctctttccc gacggacggc ccgcgcagtt catgccgcga
     5041 ggttgaagta ttcgcggcgg ccgcgcaact tgccgatccg gtccagcagc aggtgaaagt
     5101 cggcgtcgct gtcgatcgga ttcaggtgtt cggtattgac gatcagcacc ggcgcctcgt
     5161 cgtactggtg aaagaaccgc gtgtagctgt tcgcgagcgc agcaagatac tcctcgctga
     5221 tgcgagcctc tgccggattg ccgcgccgta cgacgcggtc ggccagggtc tcggggcttg
     5281 cctgcaggta gatgacgaga tccggtgccg gggtctgtgg ccgcagttgc gccaggatct
     5341 gctcgtacaa cttcagttcg tcgtcggcca gcgtgagccg agcgaatagc ggatccttgt
     5401 cgagcaggaa atcggcgaac agcacggcgc ggaacatgtc gagctggcga acgtcggcca
     5461 gctgctgcac gcgctggaac aggaagaaga gctgagtcgg caaggcgaaa cgcgcccggt
     5521 cgccgtagaa ccgctcaaga aacgggttgg cctcgggccg ctccagcagc gtgtgtgcgt
     5581 tgaagtgctg agcaagcctg cgcgtcaacg aggtcttgcc cgcgccgatc ggtccctcga
     5641 tggcgacata gcggtagcgg ccggcgatca tctaccgggg tgggcgaagg aacgtcgacg
     5701 cgtcgacgac cggcacgact gacattgaaa cggtgccggg gttatggcgc ggcggcctcg
     5761 accggttcga tcttctggtc gtcgaccgaa tcgaggaagg cggaggccgg cccctggccg
     5821 ggaatgcgga tatccggcgc caggtcgacc agcggccgca gcacgaaggc gcgcagatgc
     5881 agccgcgggt gcggcagcgt gagcggtgtg ctctggatga tcatgtcgcc cagcagcagc
     5941 aggtcgagat cgacattgcg aggcgcattc ttcttgagtc cgcggcgctt gcggccgagc
     6001 atcagctcaa tgtccagcag atgcagcagc agcgcataag gctcgagcga ggtttcgacc
     6061 ttgacgaccg cgttgaggta gtcgggtccg gtcgcgtcga tcggggcgga gcgatagtac
     6121 ggcgagaccg caacgaactc cgtctgctcg atcgaacgca gcgactcgat cgccgagttc
     6181 agcgcctcga cgacgtcacc ctggttcgat cccagcccga tgtaagcgat ccgtttcact
     6241 gccgatattc gccgtcgaag gtcaagccat ccccgtttcg ctgctcgtgt ccgtcgccgc
     6301 gtgcgacgca ccgcccttgc cccgcgtgcg gcgccgcctc ttgcgccctg gagcttccgt
     6361 cacacccgac cgcgctgcgt cttccaccag tcgttcgcgt tcttcttcgt tcgcgtgcga
     6421 aaaacgggtc caccagtcgg ccaactcggc cggaatctcg tcggcggccg cccgcagcag
     6481 caggaaatca taaccggccc gaaatcgcag gtgctcgaag aggcggaacg ccgaccgccc
     6541 gctgcggcgc tcgaagcgag gctggaggcc ccagatctcg cgcatgtcgg agacgaaccg
     6601 tcgctggact gacagtttct cggtctgggc atcgagcgtg tcgtcgatgg cggcgttgag
     6661 cgccgggatg cgatgctcgc cctgggccgt gcgtgcattc catttgacca gcacgtcgtg
     6721 ccacagcaac gtggcgaata gaaaccccgg cgagatcgac ttcccgccca gcacgcgctc
     6781 atcggtgcgc gcaagtgcct gcaggacgaa gcgctccccg gccggctgct cgaggatcac
     6841 gtcgagcagc ggcagcaggc cgtggtgcag gccttccatg cgcagccgcg tgatgcacgc
     6901 gaccgagtgt ccgctcgtca gcagcttcag catttcgtcg aagaggcgcg ccgtcgggac
     6961 gttctcgatc aggcgtgcca gccgcggaat cggctcgcgc gtcgcttcgt cgatgtcgaa
     7021 gccgagtttc gcggcgaagc gaaccgcgcg cagcatgcgc accggatctt cgcggtagcg
     7081 cgtctcggga tctccgatca tccgcaagcg cttccggcgg acgtccttca cgccatcgtg
     7141 atagtcgatg acctcgcgtg tcgtcgggtc gtagtacagc gcgttgaccg tgaagtcgcg
     7201 ccgcgtggcg tcctcgtgct gttcaccgaa caggttgtcg gccagcacgc gcccgtgctc
     7261 gtccgtcttc cggtccgggt tgtccagtgc cctgaaggtc gaaacctcga tcgtctcctg
     7321 gccgaacatc acgtgcacga gccggaagcg ccggccaatg atgatggcgc ggcggaagtg
     7381 cgacttgacc tgctcgggcg tggcatcggt tgcgatatcg aagtccttgg gcgccacccc
     7441 gagcagcagg tcgcgcacgg cgccgccgac aaggtaggcg cgatgcccgg ctttctgcag
     7501 cgtttcgcat acccgcagcg ccgaccgggc gaccaggtcc tggtcgatgc cgtgctcggc
     7561 ggcagggacg cgcttgggct cgcgcggctt gcgcggcttg cgcgagaaca gcgacgaaac
     7621 gcgtgcgatg acgcgtctga tcatgcgatc ctcgcgcgca gggtcggggt gaggtcaggc
     7681 atgggcggga tcgaaaagct gcagggttct ccagccccgt tcgcgcgcga tggcaagcag
     7741 gcgcgcatcg gcgttcgtca cgactgggtg ggtcactttc tccagcagcg gcagatcgtt
     7801 gatcgaatcg ctgtagaacg tggtgatcgt gtcggcgagc gtgtgccctc gggccgcgag
     7861 ccactcctcc accttgcgga ccttgccgcc ctggtaggtg tgggtgccga cgtgtccgcc
     7921 ggtgaagcgt ccgttgtcct gttcaggctc gacggcaagc acgtggtcga tcccgaaaga
     7981 ccgcgcgatc ggcgtcacga cgtaggcatt ggtgccagtg accagggcca gttcatctcc
     8041 ggcagcgcgg tgccgctcga ccagtgcacg cgcctgttcg cggatcgccg ggcttacgtg
     8101 tcgttgcatg aactcgcgat gccagtcgtc cagcgttgcg cgcggaaacg ctgccagcag
     8161 ttccatctgg aaggccacgt aggcatcgac gtcgaaggtg ccggcgcgat actcgtcggc
     8221 aaaccggcgg atgcgggcgc cgtagtcggc ggagtcgagt ccgccacgct cgacgacatg
     8281 gtgcgaccac gtgtcggcac tgtcgatcgg cagcagtgtg tgatcgaggt cgaaaagcgc
     8341 aagatgcatc ggcgcgattc tacgcactcg ctcttgttcg caagccctgt gctaagcatc
     8401 ggcctgtgct gcccactcgc gcacgagcgg cacggtaatc gcgcgcttgg cctccagcgc
     8461 ataggcgtcg agcgcattga ggatggcgac ctgagtgctc aggtcgcgcg gcatgtgggt
     8521 cagcaggtag ccgagcacgt cggccgacag atcgatgccg cgtgaagcag cgtgtgcgcg
     8581 cagcgcgtcc tccttctcgg cgtcggtgag gccgtgaagc tggtagacga gaccccaggc
     8641 gagccgcgtg cggacgtctt cgcgcagcac gagctgggac ggcggcgcac tgccgctggc
     8701 gaccagacca ctatcgcgcg acgcgcggat ctcgttttgc agcacgaaga gttgctgctg
     8761 gcgcatcggg tcgtaacgat cgacgtcgtc cgccaggtag agctgaactg cggggtcgaa
     8821 ctccagcgtt gaactcgcac tgtcgagcca gcggctggag gcgctggcgg caacggcctg
     8881 cagcaggtgc gtgcggccgc tgccttcacc gccccagagg tagacgaacc gctcggcgcc
     8941 gccggcggcg agggtgcgca gagcagcgat cacctcggca ttgcggccta cgacgaagtt
     9001 atccagcgaa gggcgcagcg gctggatcag gtcgagcgcc agctgttggt ggggtgaatt
     9061 ccgatacggt gaactcatca gtcgacggag cgaacacggc tgggccgcgc cttacaatcc
     9121 gctttccctt cctaggtgca ctgagcacgc ggccagcggg aagtctaccg ggccacgcga
     9181 ggtcgacccg tccatgaatc cactcgacta caagcaggcg ggcgtcgact acgccagtat
     9241 cgaccccctg aagatcttgg cgcagcaggc cgcggcgggc accgccggca accttgcgcg
     9301 tcacggtctg atcgaggttg cagcgtcgcg gggcgagtcg gcctatgtgg tcgactgtgg
     9361 tgacttctac ctggcctcga tcactgaatg cctcggcacc aaggcgctgg tcgccgacgc
     9421 gatgcgggcg atcaccggcc gcacgtacta cgaccagatc gcgcaggaca cgctggcgat
     9481 ggcgctgaac gacatcatca ccgtcggcgc gacgccagtg tcggtacatg cctattgggc
     9541 gacggggggc agcgactggt tcgccgacgc ggcccgcgcg cgcgatctgg tggacggctg
     9601 gcgcgcggct tgcgatcgca gcggcgtggc ctggggcggc ggtgagacgc ccgcattggc
     9661 cggggtggtc gagtccggcc gcatcgacct ggccgcgtcg tgcgtcggac tggtccgccc
     9721 gaagtcgcgt ttgacgctgg gtgaacggct gggtgacggt gatgcaatcg tcctgctggc
     9781 atcgagcggc atacacgcca atggcgtttc ggtggcacgc aagctggccg agcgacttc
//
LOCUS       DATIRT010000476         1681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1360797, whole genome shotgun sequence.
ACCESSION   DATIRT010000476 DATIRT010000000
VERSION     DATIRT010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360797"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VLN25_10965"
     CDS             <1..739
                     /locus_tag="VLN25_10965"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSQ11112.1"
                     /translation="GRINGEQALFGEAGIIPWVKYFSNDNPLYDISNKVGALAAAQAS
                     AAETEAILEIGGGLGSGAIALLDHFESIGRTAQIGSYTLTEISPLFLKRAQRNLAAHN
                     PRCALRFAALDINQPFALAGIAPGSVSMVYGVNVLHVARDLGKTLAELRGALRDHGLL
                     VMTECIRPFANVPLHLELIFNLLGSFRDALLVPEWRPNGGFLTPEQWTAALEANGFRD
                     VRIYPDIAAIRDAYPSCVIGALLARRS"
     gene            736..1530
                     /locus_tag="VLN25_10970"
     CDS             736..1530
                     /locus_tag="VLN25_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11113.1"
                     /translation="MNAAELKATLAIPAGGPWFEGHFPGRPILPGVAELALAIAALRK
                     ETGKPLPLRGIAFARLRHLVLPGDRLELSARESIGGAGDTGRLRFDLKRDGALVANGE
                     FMVGTPLRAEAAPAIVARSPPGDTDTAPEALLPHHPPMRFLTSILWQDAETLDGTACI
                     PNRCALVSDGSAPTVAAIEAAAQAAAAWEALLRRHRQGGTAPRVGYLVAMRDVDFFTD
                     RIPADKSLLVAVHLEAASPPLTHYRFEVSLEGLLLVRGSIGTFLAT"
BASE COUNT          297 a          549 c          528 g          307 t
ORIGIN      
        1 cgggcggatc aacggtgaac aggccctgtt cggcgaggcc ggcatcatcc cgtgggtgaa
       61 atacttctcc aacgacaacc cgctgtacga tatcagcaac aaggtcggag cgcttgcagc
      121 ggcgcaggcg agcgcagcgg aaaccgaagc aatcctggaa atcggcggcg ggctcggcag
      181 cggcgcgatt gcactgcttg accatttcga gagcatcggc cgcacggcgc agatcggctc
      241 ctacaccctc accgaaattt cgccgctgtt tctgaagcgc gcccagcgca atctcgccgc
      301 ccacaatcca cgttgcgcac tgcgattcgc ggcgctcgac atcaaccagc cgttcgcact
      361 ggccggaatc gcgccgggca gcgtttccat ggtctacggc gtcaacgtgc tgcatgtcgc
      421 gcgggatctg ggcaaaacgc tggcggaact gcgcggcgcc ttgagggatc acggcttgct
      481 ggtcatgacc gagtgcatcc gtccgtttgc gaatgttccg ctgcatctcg aactgatatt
      541 caatcttctg ggctcgttcc gcgatgcgct gctggtgccg gaatggcgcc cgaatggcgg
      601 cttcctcacc ccggaacaat ggaccgcagc gctggaagcc aacggctttc gggacgtgcg
      661 aatctatccc gatatcgcgg cgattcgcga cgcctatccg agctgcgtga tcggggcgct
      721 cctggcccgg cggtcgtgaa cgcggcggag ctgaaggcga cactcgcgat acccgccggc
      781 ggtccttggt tcgaaggaca ttttccggga cggccgatac ttcctggcgt ggcggaactg
      841 gcgcttgcca ttgcagcgct cagaaaggaa acgggcaaac cgctgcctct acgtggcatc
      901 gctttcgcac ggctgcgcca tctggtgcta ccgggagatc ggctcgaact gtcggcccgc
      961 gagagcatcg gtggggcggg cgatacgggg cggttgcgct tcgacctgaa gcgcgatggc
     1021 gcgctggtgg caaatggcga gttcatggtc ggcacgcctc tccgcgccga agccgccccg
     1081 gccatcgttg cgcgtagccc accgggcgac accgacaccg cgccggaagc cctgctgccc
     1141 caccacccgc cgatgcgctt tctgacctcg atcctttggc aggatgccga gaccctcgac
     1201 ggcacggcgt gcattccgaa ccggtgtgcg ctcgtttccg atggcagtgc gccgactgtt
     1261 gccgccatcg aggccgctgc ccaggccgca gcggcatggg aagcgctgct gcggcggcac
     1321 cggcagggcg gcaccgcacc gcgcgtgggc tatctggtcg ccatgcgcga cgtggacttc
     1381 ttcaccgacc gcattcccgc cgataagtcg ctgctcgttg cagtacacct cgaagccgcc
     1441 tcccctccgc ttacgcatta ccggtttgaa gtctcactcg agggcttgct tctcgtccgc
     1501 ggctctatcg gtaccttcct cgctacgtag ccagtcttga aaaggtcaaa gtcaaaagcg
     1561 ttcaccgcaa aggacgccag agacgccaag gcaattcaat tgcagaaagc aggtggacat
     1621 tgaaaccttg accgggtgat ttcggtgcga ccggcccgta cttctacggt tcagaaatgg
     1681 g
//
LOCUS       DATIRT010000477         3420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_476742, whole genome shotgun sequence.
ACCESSION   DATIRT010000477 DATIRT010000000
VERSION     DATIRT010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476742"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VLN25_10975"
     CDS             complement(<1..722)
                     /locus_tag="VLN25_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HSQ11114.1"
                     /translation="MLPAAVVLLLFLTYPLGLGTWLGFTDTKIGRGGEWIGLENYEFL
                     WGDPITRLSVFNTIFYTVVASIIKFALGLWLALLLNKHLPFKSFIRAIVLLPFIVPTA
                     LSAIAFWWIYDAQFSIISWTLVKLGWIDRYIDFLGEPWLARLSVIAANIWRGVPFVAI
                     CLLAGLQTISPSYYEAASLDGATDWQQFRFVTLPLLTPIIAVVMTFSVLFTFTDFQLI
                     YVLTRGGPLNSTHLMATLSFQRA"
     gene            complement(893..2212)
                     /locus_tag="VLN25_10980"
     CDS             complement(893..2212)
                     /locus_tag="VLN25_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HSQ11115.1"
                     /translation="MKNLKRREFLKTSAGVAAAAAGPLIWVKDAEAQWSNAPEKGAKL
                     RVLRWKRFVQGDEDQYLANVKKFSEKYGIEVRVDAEGWEDVRPKAAVAANTGAGPDII
                     LSTNDDANLYPDKLVDVTDLCTYLGKKYGGFYPVCDPYLRPDGKKWIGVPLGVAGVCM
                     VYRISHVKAAGFDTFPKDTDGFLKLCKALKDKGTPPGFALGNATGDGNTWAQTLVWGF
                     GGKLVDANNKVVIDSPETIKALEYVKEMYSSFVPGTLSWLDPNNNKAFLDEQISLTNN
                     GISIYYAAKTSKDPKVAALAADINHANMPIGPIGKPSEFQLFFNQMIFKYTKYPKAAK
                     EFLRFMMEAEQMDPWVQAAIGYVTPALTYYEKNPIWTVDPKHTPYRDSMKNMWPSGHA
                     GKMGYASAGALADFIVVNMVAEAASGSKTPKEAADRAQKRAERYYKV"
     gene            complement(2243..3331)
                     /gene="ugpC"
                     /locus_tag="VLN25_10985"
     CDS             complement(2243..3331)
                     /gene="ugpC"
                     /locus_tag="VLN25_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HSQ11116.1"
                     /translation="MAQVAIRGVAKRFGGTTVIHGVDIEIAEGEFAVLVGPSGCGKTT
                     LLRMIAGLEEISDGEILIGDRMVNRVPPKERDIAMVFQNYALYPHMTVRDNMSFSLRL
                     AKASKDEIDRRVGRAADILGLGKLLDRYPRQLSGGQRQRVAMGRAIVRDPQVFLFDEP
                     LSNLDAKLRVQMRTEIKELHQRLKTTSVYVTHDQIEAMTMADKIVVMKDGVVEQTGGP
                     LDLYDNPANTFVAGFIGSPAMNLLPGKVQRQGGALEAEFADGSRLPLAKSAGVREGQA
                     VLYGIRPEHFGVGSSGGLAVEVVVVEPTGADTQIYCRFVDQEVTATIRDRTNVRAGER
                     ITLVPDAGRAHLFDAASGKRLATGFQPA"
BASE COUNT          612 a         1084 c         1064 g          660 t
ORIGIN      
        1 gcgcgctgga aactgagggt ggccatcagg tgggtcgaat tcagcggccc gccgcgcgtg
       61 agcacgtaga tcaactggaa atcggtgaag gtaaacagca ccgaaaaggt catcacgacc
      121 gcgatgatcg gtgtcagcaa cggcagcgtg acgaagcgga actgctgcca gtcggtggcg
      181 ccgtcgaggc tggccgcctc gtaatacgac ggcgagatcg tctgcagacc cgccagcagg
      241 cagatcgcga caaagggcac gccgcgccag atgttggcgg cgatcaccga caggcgcgcc
      301 agccagggct cgccgaggaa atcgatgtag cggtcgatcc accccagctt gacgagcgtc
      361 cagctgatga tcgaaaactg cgcgtcgtag atccaccaga acgcgatggc agagagcgcc
      421 gtcggcacga tgaacggcag cagcacgatc gcacggatga aggacttgaa cggcaggtgc
      481 ttgttgagca gcagcgccag ccacaggccg agcgcgaact tgataatgct ggcgacgact
      541 gtgtagaaga tcgtgttgaa aaccgagagc cgggtgattg gatcacccca gagaaattcg
      601 tagttctcaa gcccgatcca ctcgccgccg cgtccgatct tggtgtcggt gaaaccgagc
      661 caggtgccga ggccgagcgg ataggtcagg aacagcagca gcacgaccgc cgccggcagc
      721 atgaacacca gtccgaggaa gttccggctt tcgagcaggc gactcagtac tccggcctca
      781 tggacgggta cctggactcg ggcgctgttc acggtgatca ggggttaggg tcggaacgcg
      841 gagaagtgca tgacggcgcc cgctgcggca ccgccatgca cgaccgacgc gtctagacct
      901 tgtagtagcg ctcggcccgt ttctgcgcgc gatccgcagc ttccttcggc gtcttcgatc
      961 ccgacgcggc ctcggccacc atgttgacga cgatgaagtc ggccagcgcg ccggccgacg
     1021 catagcccat tttgccggcg tgtccgctcg gccacatgtt cttcatcgag tcccggtacg
     1081 gcgtgtgttt cgggtcgacg gtccagatcg ggttcttctc gtagtacgtc agcgctggcg
     1141 tcacatagcc gatcgccgcc tgtacccacg gatccatctg ctcggcttcc atcatgaagc
     1201 gcaggaactc cttggccgcc ttcggatact tcgtgtactt gaagatcatc tggttgaaga
     1261 acagctggaa ctcgctgggc ttgccgatcg gcccgatcgg catgttggcg tggttgatgt
     1321 ctgccgccag cgcggcgacc ttggggtcct tcgacgtctt cgcggcgtag taaatggaga
     1381 tgccgttgtt cgtgagcgat atctgctcgt cgaggaacgc cttgttgttg ttcgggtcca
     1441 gccaggacag ggtgcccggc acaaacgacg agtacatctc cttgacgtac tcgagcgcct
     1501 tgatggtttc cgggctgtcg atgacgacct tgttgttcgc atccacaagc ttgccgccga
     1561 acccccacac cagcgtctgt gcccaggtgt tgccgtcgcc ggtcgcattg ccgagcgcga
     1621 acccgggcgg tgtgcctttg tccttcagcg ccttgcacag cttcaggaag ccgtctgtgt
     1681 ccttcggaaa tgtgtcgaaa cccgcggctt tcacgtggct gatgcgatac accatgcaca
     1741 cgcccgcgac accgagcggc acgccgatcc acttctttcc atccggtcgc agatagggat
     1801 cgcacaccgg atagaagccg ccgtacttct tgccgagata agtgcacagg tcggtgacgt
     1861 cgaccagctt gtcggggtac aggttggcgt cgtcattggt cgacaggatg atgtccggac
     1921 cggcaccggt attggccgcc acagccgcct tcgggcgcac gtcttcccag ccctcggcgt
     1981 cgacgcgcac ttcaatgccg tacttttccg agaacttctt gacgttggcc agatactgat
     2041 cctcgtcgcc ctgcacgaag cgcttccaac gcagcacacg cagcttggcg cctttctcgg
     2101 gcgcgttgct ccactgggcc tcggcatcct tgacccagat gagtgggccg gccgcggcgg
     2161 ccgccacgcc tgccgacgtc ttcagaaact cccgccgttt cagattcttc atacacgtct
     2221 cctttgtggt tggctcacat tgtcaagccg gttgaaatcc cgtcgcgagg cgctttccgc
     2281 tcgcagcgtc gaaaagatga gctcgcccgg catccgggac gagcgtgatg cgttcgccgg
     2341 cgcgcacgtt ggttcgatcg cggattgtcg cggtcacttc ctggtcaacg aagcggcagt
     2401 agatctgggt atctgcaccg gtcggttcga cgacgaccac ttcgaccgcg aggcccccgc
     2461 tgctgcccac cccaaagtgc tcgggccgga tgccgtacag gacggcctgt ccttcgcgca
     2521 cccccgcgct tttcgccagc ggcagtcggc ttccatcggc gaactccgct tcgagcgcgc
     2581 cgccctgacg ctgcactttc cccggcagca ggttcatggc gggcgatccg atgaagcccg
     2641 ccacgaacgt gttcgcgggg ttgtcgtaga ggtcaagcgg gccgccggtc tgttcgacga
     2701 cgccgtcctt catgacgacg atcttgtcgg ccatggtcat cgcctcgatc tggtcgtgcg
     2761 tcacgtagac cgaggtggtc ttcagtcgct ggtgcaactc cttgatctcg gtgcgcatct
     2821 gcacgcgcag cttggcgtcg agattggaca gaggctcgtc gaacaggaac acctgcgggt
     2881 cgcgcacgat ggcgcgcccc atggcgacac gctggcgctg accgcctgag agctggcgcg
     2941 gatagcggtc gagcagcttg ccgagcccaa ggatgtcggc cgctcgtccg acgcggcggt
     3001 cgatctcatc cttcgaggct ttcgcaagcc gcaggctgaa cgacatgttg tcgcgcaccg
     3061 tcatgtgcgg atagagcgcg tagttctgaa agaccatcgc gatgtcgcgc tccttcggag
     3121 gaacgcgatt caccatgcga tcgccaatca ggatctcacc gtcgctgatt tcctcgagcc
     3181 cggcgatcat gcgcagcagc gtggtcttgc cgcagccgga gggacccacc aggaccgcga
     3241 actcgccttc ggcgatttca atgtcgacgc cgtggatcac ggtcgtccca ccgaatcgct
     3301 tggcaacgcc gcgaatcgcg acttgcgcca taaagcgttg tctcctccgc gcggcaggca
     3361 aggtgccgct gcgatcaacc tgccgggatc ttcgtccctt gaatcacagg atacatgggg
//
LOCUS       DATIRT010000478         3147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_772082, whole genome shotgun sequence.
ACCESSION   DATIRT010000478 DATIRT010000000
VERSION     DATIRT010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772082"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VLN25_10990"
     CDS             <1..298
                     /locus_tag="VLN25_10990"
                     /inference="COORDINATES: protein motif:HMM:NF015746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HSQ11117.1"
                     /translation="GAIPKWAIFAPIFVPLLMKLGVPPDATLAAYRVCDSPMNSITPL
                     NAYFAMIVVFCQKYVKDAGVGTVIALMLPYVIAIAIVWLILLVAWQLVGLPWGI"
     gene            378..1868
                     /locus_tag="VLN25_10995"
     CDS             378..1868
                     /locus_tag="VLN25_10995"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HSQ11118.1"
                     /translation="MKITLSNAAPATLKSAMLVVGAFADGSLLPAAQAIDQAAGGKLA
                     AALKRGDLDEKAGSTLLFPDLGGVAAERVLLVSLGQKAALGDKAYRDAISATAKLLAG
                     SVAKDAAVTLADVEVPNRPLAWRLQQAARLLADGAYKFDAPRAKQPPARGASAVTLVV
                     ARESTAELEGAIKAGLAVSEGMALTKDLGNLGGNVCTPAYMAETAQKLGKEFGFDVEI
                     FERADMEKLGMHSALSVGRASHSPCKFIVMKHSKGGSAKPIVLIGKGMTFDTGGVSLK
                     PGANLDEMKYDMCGGGSVIGIFRTVAKLGLPINLVGLVMAVENMPGGNASRPGDVVKS
                     MAGITIEILNTDAEGRLVLCDAMTYAERFDPACVIDLATLTGACVIALGAHASGLFAN
                     DDALAAELLMAGMNSGDRAWQLPMWDDYQGQLKSNFADMSNLGGPPAGSITAALFLSH
                     FAKKYKWAHIDIAGTASVSGDAKGATGRPVPLIADFLMMRAEGKSA"
     gene            2002..>3147
                     /locus_tag="VLN25_11000"
     CDS             2002..>3147
                     /locus_tag="VLN25_11000"
                     /inference="COORDINATES: protein motif:HMM:NF018448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HSQ11119.1"
                     /translation="MNPARPAQDSGVLLEFMFRLGQAYLACGEQTAEIELLLRRVALA
                     YGMGKTRVVALPTAIFITVHDQTSERVTLAEGPSQSLRLDQIAEVYRLGDAAQRAKLT
                     PREGLDRLVEILRTQPRFGRTGALIGHTILAIGVAMVLMPTLANIAAAAILGFIIGGL
                     KLLVPERTLFAVPMPVVAATLVSATVFYAASYGLPVDPLHMLVPPLVTFLPGGMLTLG
                     MVELAYGDMVSGSSRLIAGFVQLVLLTFGLAAGAALLGTHPESLVYSSSQFVKVSWAA
                     WAGVPVFCLGVYLHFSAPRNSLVWILLVALAAIAAQRAAAGLFGAEISGFFGMIVAMP
                     MSYLIHLRFNGPPSTVTSLPSFWLVVPGSLGLLSVTRMLSDRAAGLEG"
BASE COUNT          507 a          992 c         1064 g          584 t
ORIGIN      
        1 cggggcgatt ccgaagtggg cgatatttgc gccgatcttc gtcccgttgc tgatgaagct
       61 cggcgtgccg cccgatgcca cgctggcggc ctaccgcgtc tgcgactcgc cgatgaattc
      121 gatcacgccg ctcaatgcgt acttcgcgat gattgtcgtg ttctgccaga agtacgtgaa
      181 ggatgccggc gtcggtacgg tgatcgcact gatgcttccg tacgtgattg cgatcgcaat
      241 cgtctggctg atcctgctgg tggcctggca actcgtcggt ctgccctggg gtatctagag
      301 cgattcgatt gagtccgaag ggcgcggcag gccgcgccct tcggcgtctg tagttttcac
      361 tttttcggag cgaccccatg aagatcactc tttccaacgc ggcaccggcc acgctgaagt
      421 ccgccatgct ggtcgtcggc gcgtttgccg acggatcact gcttcccgcg gcgcaggcga
      481 tcgaccaggc cgccggtggc aagctcgcgg cggccctcaa gcgaggcgac ctggacgaaa
      541 aggccggttc gacgttgctg ttcccggacc tgggcggcgt cgctgcggag cgggtcctgc
      601 tggtcagtct gggacagaag gctgccctgg gtgacaaggc ctaccgcgac gcgatttcgg
      661 ccactgcgaa actgctggcc ggcagtgtgg cgaaagacgc cgcagtcacc ctcgcggatg
      721 tcgaggtgcc caatcgaccg ctggcttggc gcctgcaaca agcggcacgg ctgctggccg
      781 acggcgccta caagttcgat gcgccgcgcg ccaagcagcc accggcgcgt ggagcgagcg
      841 cggtgacgct agtggtggcc agagagtcaa cggcggaact tgagggagcg atcaaggccg
      901 ggctcgcagt ctccgaaggc atggcgctga ccaaggacct cggcaacctg ggcggcaatg
      961 tctgcacacc ggcctacatg gccgagacgg cgcagaagct cggcaaggag ttcggcttcg
     1021 acgtcgagat cttcgagcgc gccgacatgg aaaaactcgg catgcattcg gcgctttcgg
     1081 tcggccgcgc gtcgcattcg ccctgcaagt tcatcgtgat gaagcacagc aagggaggca
     1141 gtgccaagcc gatcgtgctg atcggcaagg gcatgacgtt cgacaccggc ggcgtgtcgc
     1201 tgaagccggg cgccaacctt gacgagatga agtacgacat gtgcggcggc ggcagcgtca
     1261 tcggcatctt caggaccgtg gcgaagctgg gcctgccgat caatctcgtg gggctggtca
     1321 tggccgtcga gaacatgccc ggcggcaatg ccagtcgccc gggcgatgtc gtcaagtcga
     1381 tggcgggcat cacgatcgag atcctgaaca ccgacgcaga agggcgcctc gtgctgtgcg
     1441 atgcgatgac ctatgcggag cgtttcgatc ctgcctgcgt gatcgacctg gccacgctga
     1501 ccggcgcctg cgtgatcgcc ctcggtgccc acgccagcgg cctgttcgcc aatgatgacg
     1561 cgctcgcggc cgaactgctg atggcgggca tgaactcggg cgaccgggcg tggcaattgc
     1621 cgatgtggga cgactaccag ggccagctga agagcaactt cgccgacatg agcaacctcg
     1681 gcggaccgcc ggcgggttcg atcaccgccg cgctgttcct ttcgcacttc gcgaagaagt
     1741 acaagtgggc gcatatcgac atcgccggca cggcctcggt gtcgggcgat gcgaagggcg
     1801 cgacgggccg gcccgtgccg ctgatcgcgg actttttgat gatgcgcgcc gaaggaaagt
     1861 cggcctgatc gcacaccggg ttcgcagtgg tagtgcgtgg cctggcatcg gccatgcact
     1921 gccctgcctc aaaattccga agcagcccat gaaagccgat ctgcgcgcgg taccgatgca
     1981 caggcctgga atgcgcaggc catgaacccg gcccgaccgg cgcaagactc cggcgtcctg
     2041 cttgaattca tgttccggct cgggcaggcg tacctcgcct gcggcgagca gacggccgaa
     2101 atcgagctgc tgttgcgccg ggttgcattg gcctacggca tggggaaaac gcgtgtcgtc
     2161 gcgttgccca ctgccatttt catcacggtg cacgaccaga cgtccgagcg cgtcacgctg
     2221 gccgaaggcc cctcgcagtc cctgcgactg gatcagatcg cagaggtcta tcggctcggc
     2281 gacgctgctc agcgcgcgaa gctcacgccg cgagagggac tggatcgact ggtcgagatc
     2341 ctgcgcacgc agccacgctt tggccgaacc ggcgctctga tcggccacac gattctcgcg
     2401 atcggcgtgg cgatggtgct gatgccgacg ctcgccaaca tcgcggcggc cgcgatcctt
     2461 ggcttcatca tcggaggtct caagctgctg gtgccggagc gaacactgtt cgcggtgccg
     2521 atgccggtgg tcgcggcgac ccttgtgtcc gcgacagtgt tctatgccgc gtcctacgga
     2581 ctgccggtcg atccactgca catgctggtg ccgcctctgg tcacgttcct cccgggtggc
     2641 atgctgacgc tcggcatggt cgagctggcc tatggcgaca tggtgagcgg ctcgagccgg
     2701 cttatcgcgg gttttgtcca gctggtgctg ctcacgttcg gcctggcggc gggcgccgcg
     2761 ctgctgggta cgcatccgga aagcctcgtc tactcgagct cacagttcgt gaaggtgtca
     2821 tgggcggcgt gggctggggt gccggttttc tgcctcggtg tctacctgca cttctccgcg
     2881 ccgcgcaatt cgttggtctg gatcctgctg gtggcactgg cggccatcgc ggcacagcgc
     2941 gcggcggccg ggctattcgg cgccgagatc agcggcttct tcggcatgat cgtggccatg
     3001 ccgatgagtt acctgatcca cctgcgcttc aacggaccgc cctcgacggt gacatcgctg
     3061 cccagcttct ggctggtcgt accgggttcc ctcgggctgc tatcggtgac acgcatgctg
     3121 agcgaccgtg ctgccggcct cgaagga
//
LOCUS       DATIRT010000479         5110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_574937, whole genome shotgun sequence.
ACCESSION   DATIRT010000479 DATIRT010000000
VERSION     DATIRT010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574937"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..152)
                     /locus_tag="VLN25_11005"
     tRNA            complement(77..152)
                     /locus_tag="VLN25_11005"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(116..118),aa:Glu,seq:ttc)
     gene            complement(176..251)
                     /locus_tag="VLN25_11010"
     tRNA            complement(176..251)
                     /locus_tag="VLN25_11010"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(216..218),aa:Ala,seq:ggc)
     gene            complement(309..1724)
                     /gene="gltX"
                     /locus_tag="VLN25_11015"
     CDS             complement(309..1724)
                     /gene="gltX"
                     /locus_tag="VLN25_11015"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HSQ11120.1"
                     /translation="MTTVRTRIAPSPTGMLHLGTARTALFCWAFARHYGGKFTLRIED
                     TDLERSTEEAVQVILDSMQWLALDYDEGPVRQTDRLDRYRAAIERLLADGKAYYCYAS
                     REELDQLREAQMARGEKPRYDGRWRPENSRGMTPPAGVKPVVRFRNPDDGDVGWNDLV
                     KGPIAFSNTELDDLVIARGDGVPTYNFAVVVDDIDMAITHVLRGDDHVNNTPRQINIY
                     RALLGPGATLPQFGHLPMILGPDGQKLSKRHGAVSVLQYDVDGFLPEALFNYLARLGW
                     SHGDAEKFTREQMVEWFDLEHVNRAPAQYNLDKLLWLNGEYMKEADNARLARLAATRI
                     EARGGVLAKGPALERVVALLKDRSRTLNELADAALLFYRDVAREPALLAEHLQGSGRE
                     AVRMFRERAGELTWERAAISGLIKQVVGELKLKMPQVAVPLRVAVTGQTQTPSIDAVL
                     ELIGRDAVLARLDNALEQGAA"
     gene            1900..3432
                     /locus_tag="VLN25_11020"
     CDS             1900..3432
                     /locus_tag="VLN25_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11121.1"
                     /translation="MTDKLIVTNKAALRSKYGAAGLRKVMGAVAALLAADRKRGLVTR
                     LIDLSSAAMMKRLNAPVVTDALHPKQNKDAVDGLFRALEPDYLMLLGSIDVIPHQDLA
                     NPVYADDKEDDPKAWSDLPYACDAAYSRDIADFVGPTRVVARLPDLTGARDPAHLLKL
                     LATAAHVRTRPLKDYAPYFAISVRLWRRSTEQSLENIFGNDETLAISPPSSAARSRSL
                     NRLSHFINCHGGLADPQFYGEYRGKFPVAMATRHTRSKIVPGTVAAAECCYGGELYDS
                     VTLAVDEPICQSYLAHGAYAFLGSTTIAYGPARGNGAADLITQYFLIEVHKGASIGRA
                     MLDARLGYVRQSAEMDPIDLKTLAQFCLYGDPSVHPVERDSATTVPRSINPAEARRYV
                     RAERRAKMRLSGEMLQQSKPTASQASKVRPTKAVEKSLRSIARAVGVDAKARFTTFDV
                     SGGLAPARRSLTRLNAQCERYHVMIAALPGRRKCDLGGRVAIVAKEAGARVVGYRVYL
                     QK"
     gene            3465..3695
                     /locus_tag="VLN25_11025"
     CDS             3465..3695
                     /locus_tag="VLN25_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11122.1"
                     /translation="MHRLRGTVIKATFASGSKSERKAPFLQTADQRYLLRRRGGPSYG
                     DTTLTRFVGHAVECDGTVTSYLLLVDRIEQVD"
     gene            complement(3772..4359)
                     /locus_tag="VLN25_11030"
     CDS             complement(3772..4359)
                     /locus_tag="VLN25_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11123.1"
                     /translation="MRILLFSGHMVDRPDRTLPRFPANRVAAAGRAIHAELDRLGVGA
                     GDRAVTSGASGGDLLFALDCLARGAAIEIYLPFPRDRFVQSSVLPAGTRWLAMFDEVI
                     AHPLTVLNVMPPADDDAEDGNAYARCNLAMLRRALQLARGPIDLVCLWDGSSGDGPGG
                     TEHMVDAVRAHRGRVHWIDSRTLPDSTQARHPDQG"
     gene            4629..>5110
                     /locus_tag="VLN25_11035"
     CDS             4629..>5110
                     /locus_tag="VLN25_11035"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HSQ11124.1"
                     /translation="MARCPFATWRPISGSCGPNLGGPFKIVHHTTEGSSAEGALNAFR
                     SARSDPHFTVDATNIFQHIDTSEGARALRNEAGGVQTNRDSAVQIELVGFAHLPKDRR
                     ALTNLARLCRWIEATHGVAKTWPAGLPRPARNGRDPGGHNRDPQIWDSESGHYGHCHV
                     P"
BASE COUNT          898 a         1697 c         1658 g          857 t
ORIGIN      
        1 ccgaaaacgc gggacttcac aatccgctac cggctgccgg tgcctccgca ggcacgcagc
       61 cttaccggag ttaaattggc gtcccgacgg ggattcgaac cccggttacc gccgtgaaag
      121 ggcgatgtcc taggcctcta gacgatgggg acacaaggac tttttgtcgg gtggctggtg
      181 gaggtaagcg ggatcgaacc gctgacctct tgcatgccat gcaagcgctc tcccagctga
      241 gctatacccc cacggagagc cgcggagtat agccgttttc agatcagcct cgcaatcgag
      301 gcgcccctct atgcggcgcc ctgctcaagt gcattgtcga gccgagccaa cacggcgtcg
      361 cgcccgatca gttcaagtac ggcatcgatc gacggcgtct gcgtctgtcc ggtaacggcc
      421 acacgtagcg gcaccgcgac ctgcggcatc ttgagcttca gttcgccgac gacctgcttg
      481 atcaggccgc tgatggcggc tcgttcccag gtcagttcac cggcgcgctc gcggaacatc
      541 cggaccgcct cgcggccaga gccctgcaag tgttcggcga gcagtgccgg ttcgcgtgcg
      601 acgtcccgat aaaacagcag tgcggcgtca gccagttcgt tcagggtccg cgatcgatcc
      661 ttcagcagag cgaccacgcg ttcgagtgcc ggtcccttcg cgagcactcc gccgcgcgct
      721 tcgatacgcg tcgcagcaag gcgcgccagg cgtgcgttgt cggcttcttt catgtactcg
      781 ccgttcaacc acagcagctt gtcgaggttg tactgcgccg gcgcccggtt gacatgctcg
      841 aggtcgaacc actcgaccat ctgctcgcgc gtgaatttct cggcatcgcc gtggctccag
      901 cccaggcgtg cgaggtaatt gaaaagagcc tcgggcagga atccgtcgac gtcgtactgc
      961 agcacgctca ccgcgccgtg gcgcttcgac agcttctgcc cgtccggacc gaggatcatc
     1021 ggcaggtggc cgaactgtgg cagcgtcgcg cccgggccca gcaatgcgcg gtagatgttg
     1081 atctggcgcg gcgtgttgtt gacatgatca tcaccgcgca gcacatgcgt gatcgccatg
     1141 tcgatgtcgt cgacgacgac ggcgaagttg taggtcggca cgccgtcgcc acgtgcgatc
     1201 acgaggtcgt cgagctcggt gttggaaaac gcgatcggac ccttgacgag gtcgttccag
     1261 ccgacgtctc catcgtccgg gttgcggaag cgcacgacgg gcttcactcc ggccggcggt
     1321 gtcatgccac gcgagttctc ggggcgccag cgcccgtcgt aacgcggctt ctcgccgcgc
     1381 gccatctgtg cttcgcgcaa ttgatcgagt tcttccctgc tcgcgtagca gtaatacgcc
     1441 ttcccgtcgg ccagcagtcg ctcgatcgcg gcacggtacc gatcgagacg atcggtctgc
     1501 cgcaccggtc cctcgtcata gtcgagggcg agccactgca tgctgtcgag gatgacctgg
     1561 acggcttcct cggtcgaacg ctcgaggtcc gtgtcctcga tgcgcagggt gaacttgccg
     1621 ccgtaatggc gcgcaaacgc ccagcagaaa agcgccgtgc gggcggtgcc gaggtgcagc
     1681 atgccggtcg ggctgggagc gatgcgggtg cggacggtcg tcatcgggga gaggccggcg
     1741 tccgacgtgg acgccgttgg cgcgggcgcg cagtctagcg gatgatcaat gcacgacggc
     1801 gcgcccacgg cgaggcgctt cacgtcggat cgcgctttgc gacggccgtg gcgagcgcta
     1861 tggtgctgac ggcacgcgac gttgcgcaaa ggcgatggaa tgacggacaa gctgatcgtg
     1921 accaacaagg cggcactgcg ctcgaagtac ggtgccgccg gcctgcggaa ggtcatgggc
     1981 gccgtggccg cactcctcgc ggccgatcgg aagcgcggcc tcgtcacccg cctgatcgat
     2041 ctgtcgtcgg ccgcaatgat gaagcgactg aacgccccgg tggtcacgga tgcgctgcat
     2101 ccgaagcaga acaaggacgc ggtcgacggg ctgttccgcg cgctcgagcc cgactacctg
     2161 atgctgctcg gttcgatcga cgtgatcccg caccaggacc tggccaaccc cgtctatgcc
     2221 gatgacaagg aagatgaccc gaaggcatgg agtgacctcc cctatgcctg cgatgcagcg
     2281 tactcgcgcg acattgcgga cttcgtcggc ccgacgcgcg tcgtcgcgcg actccccgat
     2341 ctgaccggcg cgcgtgatcc tgctcacctg ctgaagctgc tcgcgacggc ggcgcatgtc
     2401 cgcacgcggc cgctgaagga ctacgcgccc tacttcgcga tctcggtgcg gctgtggagg
     2461 cgctcgaccg aacagagtct cgagaacatc ttcggcaacg acgagacgct ggcgatctcg
     2521 ccacccagca gcgccgcgcg cagccgttcg ctgaatcggc tgagccactt catcaactgc
     2581 catggcggcc tggcggaccc gcagttctac ggcgagtatc gcggtaagtt tccggtggcc
     2641 atggcgacac gccacacgcg cagcaaaatc gtcccaggca cggtcgcggc agccgagtgt
     2701 tgctacggcg gtgaactcta cgattcggtg acgctcgctg tggacgagcc gatttgtcag
     2761 agctacctgg cgcacggcgc ctatgccttc ctcggcagca cgaccatcgc ctatggtcct
     2821 gcgcgcggca acggcgcagc tgacctcatc acgcagtatt ttctgatcga ggtccacaag
     2881 ggcgcctcga tcggtcgggc aatgctcgat gcacggctgg ggtacgtgcg acagtcggcc
     2941 gagatggacc cgatcgacct caagaccctg gcgcagttct gcctctacgg cgacccgagt
     3001 gtccatccgg tcgagcgcga ctcagcgacg accgtgccgc gcagcatcaa tcctgctgag
     3061 gcccgccgct acgtgcgggc cgagcggcgc gccaagatgc gcctgtccgg cgaaatgctg
     3121 cagcagagca aaccgacagc ctcccaggcc tcgaaagtgc ggccgaccaa agcggtggaa
     3181 aagtcgctgc gttcgatcgc gcgcgccgtc ggggtcgacg ccaaggcgcg cttcacgacc
     3241 ttcgacgtca gtggcggcct cgcgccggct cggcgctcgc tcaccaggtt gaatgcacag
     3301 tgcgagcgct atcacgtgat gattgccgcg ctgcccgggc gacgcaagtg cgatctcggc
     3361 gggcgcgtcg ccatcgtcgc gaaggaagcg ggcgcacgcg tggtcggcta ccgcgtctac
     3421 ctgcagaagt gaagcgcgca aacaccgttg acgccccgcg cgctgtgcac agactgcgcg
     3481 gtactgtcat caaagctacg tttgcgagtg gcagcaagag cgagcgaaag gcgccgttcc
     3541 tgcagactgc cgatcagcgc tacctgttgc ggcggcgagg cggccccagt tacggcgata
     3601 cgacgctcac gcgcttcgtc ggtcacgccg tggagtgcga cggaacggtg acgtcctacc
     3661 tgctgctggt ggatcgcatc gagcaggtcg actgatggcg tctggattca agccaggaac
     3721 cccggataca cagcgaccgc agtcgccacc agcggcctcc gggcgcagcc ctcatccctg
     3781 atccgggtgt cgcgcctgcg tcgagtccgg caacgtgcgg ctgtcgatcc agtgcacgcg
     3841 accacgatgc gcacggaccg cgtctaccat gtgctcggtg ccgccaggcc cgtcaccgga
     3901 actgccgtcc cacaaacaga ccagatcgat cggtccgcgt gccagttgca gcgcccgtcg
     3961 cagcatcgcc aggttgcagc gcgcgtaagc gttgccgtcc tcagcgtcgt catcggccgg
     4021 aggcatcacg ttcagcaccg tcagcggatg cgcgatgact tcatcgaaca ttgccagcca
     4081 gcgagttccg gccggcaata ccgatgactg cacgaagcgg tcgcgcggaa acggcaggta
     4141 gatctcgatg gcagcgccgc gcgccaggca atccagtgca aacagcagat cgccgccact
     4201 ggcgccgctg gtgacagccc ggtcccccgc cccgacaccg agcctatcga gttccgcatg
     4261 gatggcacgc cctgccgccg cgacgcggtt cgcgggaaac cgcggcagcg tgcgatcggg
     4321 ccgatcgacc atgtgaccgc tgaacagcaa tatgcgcatg gaaactcagg acataggccg
     4381 gacgggaatt ctgcgcggcc ggacggcacc gcagccagag gatgcctgaa tggcgcccgg
     4441 ttggcagagg cagcggatgg atggaccggt ggcccgatcg gaaacagcga cgcttaccaa
     4501 gttgcggcga ttgcactggc gggttccgcc gcttcactga ctgcgcgccg ctggcagcag
     4561 gcgcctacac tctttgctgc agtcaattgc agtcgacccc atccccgtcc ccagcactgg
     4621 aggtctaagt ggcacgatgc ccattcgcga cgtggcgtcc catttcggga tcatgcggcc
     4681 ctaacctcgg cggccctttc aagatcgtcc atcacacgac ggagggaagt tctgccgaag
     4741 gcgcgctgaa cgccttccgt tcggcccgtt cggatcctca cttcaccgtg gatgcgacga
     4801 acatctttca gcacatcgat acgtcggaag gtgcgcgagc actgcgcaat gaagccggag
     4861 gcgtgcagac caatcgcgat tcggcggtac agatcgagct cgtgggtttt gcgcacctgc
     4921 ccaaggatcg acgcgcgctc acgaacctgg cgcgcctgtg ccgctggatc gaggcgactc
     4981 acggtgtcgc aaaaacgtgg cccgcgggcc tgccgcggcc cgcacgcaat ggtcgcgatc
     5041 cgggcggaca caaccgggac ccgcagatct gggactccga gagcgggcac tacggtcact
     5101 gccacgttcc
//
LOCUS       DATIRT010000480         1960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_83636, whole genome shotgun sequence.
ACCESSION   DATIRT010000480 DATIRT010000000
VERSION     DATIRT010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83636"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1960)
                     /locus_tag="VLN25_11040"
     CDS             complement(<1..>1960)
                     /locus_tag="VLN25_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSQ11125.1"
                     /translation="IIEGALLTVIIVFLFLGSWRSTVITGLTLPISVIATFIVIYALG
                     FTLNYMTLMALSLCIGLLIDDAIVVRENIVRHIHMGKDHYTAAREGTDEIGLAVLATT
                     LSIVAVFVPIAFMSGIIGKFFYPFGITVAAAVLVSLFVSFTLDPMLSAIWRDPPEGAR
                     GLPVVGPILRAFERGIDKVHAIYDRLLRWALAHRKSTLAIAALSFMASIPIAGMVGTE
                     MMPEADQSYTSVRLTMPVGSSLDYANERVKRVEEALREFKEIDAVDTGIGIDGTRNMA
                     TLNLKLVPRSERARSQQQLEQAIRKRVATIPGVDLKVGWNTPIYVALLGNNDAEMNRV
                     IADLKSKVQQIRGVTDVEISVKEGTPALSVRLKPELAAEYGITHAQLGTTLRAMVGGE
                     NSGYWLAPDGQNYEVITQLPQANRTIVDDIANLNIATGRQLTDGSPEVIALRSIATLE
                     RTFNPENIRRQDLQRRVALFANVQGRPAGDAGQEVQALVKTYELPPGLRFDVGGQIRE
                     QQEINAAIIGALLLAIIFIYLVLASQFGSFFHPVAIMASLPLSIVGVMLALLVTGTTL
                     NIFSMIGIVFLMGLVTKNAILLVDFANRGQREGLDRHAALLAAGQVRLRPILMTTAAM
                     IFGMLPLAIGLGEGAEQQAPMGRAIIGGV"
BASE COUNT          365 a          636 c          614 g          345 t
ORIGIN      
        1 acgccgccga tgatggcgcg tcccatcggt gcctgttgct ccgcgccctc gcccagcccg
       61 atcgccagcg gcagcatgcc gaagatcatc gcggctgtcg tcatcaggat cggccgcagt
      121 cggacctggc cggcagcaag caaggccgca tgccggtcga ggccctcgcg ctgcccgcga
      181 ttggcaaagt ccaccagcag gatcgcattc ttcgtgacca ggcccatgag gaacacgatg
      241 ccgatcatcg agaaaatgtt gagtgtcgtc ccggtgacga gcagcgccag catcacgccg
      301 acgatcgaca gcggtagcga cgccatgatc gcgacagggt gaaaaaagct gccgaactgc
      361 gaggccagga ccaggtagat gaagatgatc gccagcagca gcgcaccgat gatggccgcg
      421 ttgatctcct gctgctcgcg gatctgtccg ccaacatcga aacgcagtcc cggcggcagc
      481 tcgtacgtct tgacgagcgc ctggacctcc tggcctgcgt cacccgccgg ccgtccctgc
      541 acattggcga acagcgccac gcgccgctga aggtcctggc gacggatgtt ctcgggattg
      601 aaggtccgct ccaaagtcgc gatggaccgc agagcaatca cttccggcga gccgtctgtc
      661 agttgccggc cggtcgcgat gttgagattc gcaatgtcgt cgacgatcgt gcgattcgcc
      721 tgcggcagtt gcgtgatcac ttcgtagttc tggccgtccg gcgcgagcca gtagccggaa
      781 ttctcgccac cgaccatcgc acgcagcgtc gtgcccaatt gggcatgcgt gatgccgtac
      841 tcggcagcca gttccggctt caggcgcacg gaaagcgccg gcgtgccctc cttcaccgag
      901 atctcgacat ccgtcacgcc gcggatctgc tgcactttgc tcttcaggtc agcgatgacg
      961 cgattcatct cggcgtcgtt gttgccgaga agcgcaacgt agatcggcgt gttccagcca
     1021 accttgaggt cgactcccgg aatcgtggcc acgcgcttgc ggatcgcctg ttcgagctgc
     1081 tgctgcgagc gcgcacgctc gctgcgtgga acgagcttga ggttcagtgt cgccatgttg
     1141 cgcgtcccgt caatgccgat cccggtgtcg accgcatcga tctccttgaa ctcgcgcaag
     1201 gcctcttcga cccgcttgac gcgttcgttc gcatagtcga ggctggagcc gaccggcatc
     1261 gtcagccgca ccgacgtgta gctctggtcc gcctccggca tcatctcggt gccgaccatg
     1321 ccggcgatcg ggatgctcgc catgaagctg agcgcggcga tcgccagcgt ggacttgcga
     1381 tgtgcgagcg cccagcgcag caggcggtcg tagattgcgt gcaccttgtc gatgccacgc
     1441 tcgaaggcgc gcaggatcgg ccccacgacc ggcaagccgc gcgcgccctc gggcgggtca
     1501 cgccagatgg ccgacagcat cgggtcgagc gtgaagctca cgaacagcga caccaggacc
     1561 gccgccgcga cggtaatgcc gaacggatag aagaacttgc cgatgatgcc gctcatgaag
     1621 gcgatcggca caaacaccgc gacgatcgac agcgtcgttg ccagcacggc cagtccgatc
     1681 tcgtcggtac cctcgcgcgc tgcggtgtag tgatccttgc ccatatggat gtgtcgcacg
     1741 atgttttcgc gcaccacgat cgcgtcgtcg atgagcagtc cgatacacag cgacagcgcc
     1801 atcagcgtca tgtagttcag cgtgaatcca agcgcgtaga tgacgatgaa cgtggcaatc
     1861 acggagatcg gcagcgtcag cccggtgatg acagtgctgc gccaggatcc gaggaagagg
     1921 aacacgatga tgaccgtcag cagcgcacct tcgatgatgg
//
LOCUS       DATIRT010000481         3250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_773319, whole genome shotgun sequence.
ACCESSION   DATIRT010000481 DATIRT010000000
VERSION     DATIRT010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773319"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1342
                     /locus_tag="VLN25_11045"
     CDS             <1..1342
                     /locus_tag="VLN25_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HSQ11126.1"
                     /translation="VINDPYHGGTHLPDVTVITPVFDRDGREILFYVGSRGHHADIGG
                     ITPGSMPPFSRTLDDEGVRFINFRLIEGQGVDRPARFREAELMKLLTGGTHPARNPAQ
                     NVADLRAQVAANEKGREELLRMVDQFGLDVVQAYMRHVQDNAEESVRRVITRLRDGQF
                     EYRLDNGAVIHVRVTVDREQRSATIDFTGTSAQLPNNFNAPSAVCVAAVLYVFRTLVD
                     DEIPLNAGCLKPLEIIIPQGSMLNPDYPAAVVAGNVETSSCITNALYGALGVMASAQG
                     TMNNFTFGNERHQYYETISGGSGAGVRDVARGEERGFDGTDVVQTHMTNSRLTDPEVL
                     EFRYPVLLESYAIRAGSGGAGRWRGGNGGTRRIRFLEAMTASILANNRSIAPFGMAGG
                     AAGAPGRNWVERVDGSREELPHIGSAEMALGDVFVIETPGGGGYGSPPENLSPK"
     gene            1435..2706
                     /locus_tag="VLN25_11050"
     CDS             1435..2706
                     /locus_tag="VLN25_11050"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HSQ11127.1"
                     /translation="MNAPERIGLPDALATLSICYAPLIDKHRRAIGTRLTMLSAKAPG
                     YLPVGHILDQLNHIWPEGDASVLIAPLDATFDDSLLTWSPPANALLEIPTISLRDPEL
                     QRLVQRAFRQDIRMVLRGRPDVPLPPALTACFEYSLIHVSEDRRRLKDGRTRPAPPNA
                     VRRMPFVTTGVRRVVDLDEAYQRGAMASVGWPIDDTEQRPNCRLRPDQGNLMELLRVA
                     SENPDTAVIAHRIRRDPVLAFKLLRMVNSEAFETGAPVASIQRAIQVLGREKTLRWLS
                     LSLESAGEHVNALPLMRAATLRGLFLEHLAAGLPEVDDNMRDELFVTGAFSLLDRITG
                     APIPRLMGPVAMGGNVTEAIVNRRGPYAPYLALIEAIERSDPMAIRRHTDTLAIPIFD
                     CNQALLRALAATQRLDAEQEARPEEMVFAPV"
     gene            2760..>3250
                     /locus_tag="VLN25_11055"
     CDS             2760..>3250
                     /locus_tag="VLN25_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HSQ11128.1"
                     /translation="MTPHGRYYALAGPGALSAFRAARLLQRLQAVEPSVVAVSGRFVH
                     FVHATRALEKGEESRLKALLDYGERGGQKSDGIDVLVVPRLGTISPWASKATDIARNT
                     GLAAVHRIERGTIYTLELKRTLFRGRGLDAALEDRIRAVLHDRMTESVIEPSTDPAQL
                     FTEL"
BASE COUNT          559 a         1092 c         1073 g          526 t
ORIGIN      
        1 cgtgatcaac gacccgtacc acgggggcac gcatctgccg gacgtgaccg tcatcacgcc
       61 cgtgttcgac cgtgacggcc gcgagatcct gttttatgtc ggctcgcgcg gccatcatgc
      121 cgacatcggc ggcatcacgc cgggctcgat gccgccgttc tcgcgcacgc tcgatgacga
      181 aggcgtgcgc ttcatcaact tccggctgat cgaagggcag ggcgtcgatc gtcctgcgcg
      241 cttccgcgaa gcggagctga tgaagctgct gactggcgga acgcaccccg cgcgcaatcc
      301 ggcgcagaac gtcgccgatc tgcgggcaca ggtcgcggcc aacgagaaag gccgcgagga
      361 actgctgcgc atggtcgacc agttcggact cgacgtcgtg caggcctata tgcggcacgt
      421 gcaggacaac gccgaggagt cagtgcggcg cgtgatcacc cggctgagag atggtcagtt
      481 cgagtatcgg ctggacaacg gcgcagtgat ccacgtccgc gtcacggtgg accgcgagca
      541 gcgctcggcc acgatcgact tcaccggcac gtcggcgcag ttgccgaaca acttcaatgc
      601 gccgagcgcg gtgtgcgttg ctgccgtgct ctatgtgttc cgtacgctgg tcgacgatga
      661 gatcccgctg aacgcgggct gcctgaagcc gcttgagatc atcattccgc agggatcgat
      721 gctgaatccg gactatccgg cggccgtagt ggccggcaat gtcgaaacat cgtcctgcat
      781 cacgaacgcc ctgtacggcg cgctgggcgt gatggcttcg gcccagggaa cgatgaacaa
      841 cttcaccttc ggcaacgagc gccaccagta ctacgaaacc atctccgggg gctcgggcgc
      901 gggcgtccgc gatgtcgcgc gcggcgagga gcggggcttc gacgggaccg acgtcgtgca
      961 gacgcacatg acgaactcgc ggctgaccga tcccgaagtg ctcgagttcc gctatccggt
     1021 gctgctggaa agctacgcga tccgggccgg ttcaggtgga gcgggacgct ggcgcggcgg
     1081 caacggcggg acgcgccgca tccgattcct cgaggcgatg accgcttcga tcctggccaa
     1141 caatcgctcg atcgctccgt tcggcatggc cggcggcgcc gcgggcgcgc cgggacggaa
     1201 ctgggtcgaa cgggtcgacg ggtcgcgcga ggagttgccg catatcggct cggccgagat
     1261 ggcgctgggc gatgtcttcg tgatcgagac gcccggcggg ggcggctacg gcagtccgcc
     1321 ggaaaacttg agccccaaat agcgtgatcg gggcaccgat atcaccccaa ggacagggga
     1381 tgcggctgcc cataatcgct gcagctcatt ggctggacat ccacacgctg cacgatgaac
     1441 gctcccgaac gcataggact gcccgacgcg ctggccacgc tgtcgatctg ctatgcaccg
     1501 ctgatcgaca agcaccggcg ggcgatcgga acgcgcctga cgatgctgtc ggccaaggcg
     1561 ccggggtacc tgccggtagg ccacatcctc gaccagctca atcacatctg gccggaaggc
     1621 gatgcctcgg tgctgatcgc gccgctcgat gccacctttg acgactcgct gctgacctgg
     1681 tcgccgccgg ccaacgcgct gctcgagata ccgacgattt cactgcgcga ccccgagttg
     1741 caacgcctgg tgcagcgcgc gttccggcag gacatccgca tggtgctgcg cggtcgcccg
     1801 gacgtaccgc tgccgccggc gctcaccgcc tgtttcgagt actcgctgat ccacgtcagt
     1861 gaggatcggc gtcgcctgaa ggatggacgg acccggccgg caccaccgaa cgcggtgcgc
     1921 cgcatgccgt tcgtcacgac gggcgtacgc cgcgtcgtcg atctcgacga ggcttaccaa
     1981 cgcggcgcca tggcgagcgt cggctggccg atcgacgata ccgaacagcg acccaactgc
     2041 cgcctcagac ccgatcaggg caacctgatg gagttgctac gcgtcgccag cgagaatccg
     2101 gacaccgcgg tgatcgccca ccggattcgc cgcgacccgg tgctcgcgtt caagctgctg
     2161 cgcatggtca actccgaagc gttcgagacg ggcgcgccgg tggcctcgat ccagcgtgcg
     2221 atccaggtgc tcgggcgcga gaagacgctg cgctggctct cgctttcact cgagtccgcg
     2281 ggcgaacatg tcaatgcgct gccgctcatg cgcgcggcga cgctgcgggg tctcttcctc
     2341 gagcatctcg ccgccgggct gcctgaggtg gatgacaaca tgcgcgacga gctcttcgtc
     2401 accggagctt tctcgctgct cgaccgcatt actggagcac cgattccgcg cctgatgggt
     2461 ccggtcgcga tgggtggcaa cgtcaccgaa gcgatcgtca atcgccgcgg accgtacgca
     2521 ccgtacctcg cgctgatcga ggcgatcgaa cgcagcgatc cgatggcgat ccgccgccac
     2581 accgacacac tggcgatccc gatcttcgac tgcaaccagg cgctgctgcg cgcgctcgcg
     2641 gccacacagc gactcgacgc tgaacaggaa gcacgacccg aggaaatggt gttcgcgccg
     2701 gtataatcgc gacgccgatc acgcctgacg cgcgcgtcct gcgcgtttcc atctcctcga
     2761 tgactcctca cggtaggtac tacgcgctcg ccggtccggg cgcgctgtcc gcatttcgtg
     2821 cggcgcgcct gctccagcgc ctgcaggccg tcgagcccag cgtggtggcg gtgtccggac
     2881 ggttcgtgca cttcgtccat gccacccgtg ccctcgagaa aggcgaagag tcgcggctga
     2941 aagcgctgct cgactatggc gagcggggcg gccagaagtc cgatggcatc gatgtcctcg
     3001 tcgtgccgcg gctcggcacg atctcgccgt gggccagcaa ggccaccgac atcgcccgca
     3061 acacgggcct cgcggccgtg catcggatcg agcgcggaac gatctacacg ctggaactga
     3121 agcgaactct gttccgtggc agaggactcg atgcggctct cgaggaccgc atcagggccg
     3181 tgctgcacga ccggatgacc gaaagcgtga tcgagccgtc gaccgacccg gcgcagctct
     3241 tcacggaact
//
LOCUS       DATIRT010000482         4671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1854884, whole genome shotgun sequence.
ACCESSION   DATIRT010000482 DATIRT010000000
VERSION     DATIRT010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1854884"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..470
                     /locus_tag="VLN25_11060"
     CDS             63..470
                     /locus_tag="VLN25_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11129.1"
                     /translation="MMKRSLVVTLAAVAALIGSNALAQPVTARVTMEGTTIAVQPDPI
                     RITKAQGAVVIRWELPANANYSFDPQGIVINGERVAGQLRPQNQIAHCGGGPRHITCT
                     NSNSRRGTFKYTVRLLDANRRLVEKDPEIVNLE"
     gene            complement(457..3120)
                     /locus_tag="VLN25_11065"
     CDS             complement(457..3120)
                     /locus_tag="VLN25_11065"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSQ11130.1"
                     /translation="MIESTATTVTYLFSDIEGSTRLWESDPGRAARTLAWHDEVSRAA
                     VEGHRGTVVKMTGDGLHAAFDDSSDALAAVIELQLALAKPPADLASLSVRCGLHLGAD
                     QRRDNDFYGPAVNRAARIMCAAHGGQVLLSRAVADRLAGCLPPNVVLRDLGAVRLRDL
                     GSAEQLFQLVHPQLRTDFPALRSMASTPNNLAQQLNAFVGRDREMEQVRHLLANSRLL
                     TLLGMGGLGKSRLSMQVAAIVLEDYPDGVWFVELAAILEPQMVPQAAASVLGVKEEPG
                     GTALDALTRFVRDRKLLIVLDNCEHVVHACAELAKRLLQAGPQVRVLASSRDALRIAG
                     EAVFQVTPLPPPGQAEHSVESLLANDAVRLFVDRATAVQPAFRLTGKAAAAVAEICRR
                     LDGIPLALELAAARTRAMPIDVIAARLQDRFKLLVTTDRTVLPRQQTLRALIDWSYDL
                     LPPLEQRLFQRLSVFVGGWTLQAAESVGVGDFITAGDVMDLLANLVEKSLVVLDVDSG
                     RYRMLDTVRHYAAEQLTRSGDEAATRSLHLAWCMALAEHARAGLAGPEQGRWIAELDR
                     ERENLLGAHRWCDRAEGGVEQGLQLVFLLKLYWYHHGLLTLGHRVTAEALARTRADDR
                     SLARCRGLADVGQFCTFMGRYAAARGFLEESLVLAREMGDQNRIAAVLQTLGQACRGE
                     GDLTNAERYSAESIIEARAVGKPRQLASALNSHAQLLRQQGDLAGAKSLYAEAVQILL
                     GLGDHESVAIGRLNLAVVAIERGQADTAHALLGQALQVATELGSRQLGQSTLDILAGL
                     CAADGRHDAALKMFGAAEAQAERSGLKRGSADSAFLLPLIEQSQRALGDAAADMLSEG
                     KGLGYNEAVRRAAELLSPNAIASAPTSVTRD"
     gene            3256..4360
                     /gene="prfB"
                     /locus_tag="VLN25_11070"
     CDS             join(3256..3336,3338..4360)
                     /gene="prfB"
                     /locus_tag="VLN25_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588915.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HSQ11131.1"
                     /translation="MDAEHLNQIEGTIADLRGRLAELRRYLDVDVKETRLEEINRALE
                     DPKVWDDSKRAQEFGREKKSLDLIVVKMRTLTDGVRDAGELFEMARSDDDDATLRSVD
                     ADVTTLAKQVADLEFQRMFNNPLDPNNCFIEIQAGAGGTEAQDWAGMLERMYLRYAER
                     RGFKPELLDESQGEVAGIKGATIKISGDYAYGMLRTETGIHRLVRKSPFDSNARRHTS
                     FASVVVYPEVDETVDVEINPADLRIDVYRASGAGGQHVNKTESAVRITHAPSGIVVQS
                     QNDRSQHRNRAECMAMLKSRLYELELRKRMAEQNKLEDAKTDIGWGHQIRSYVLDQSR
                     IKDLRTNVEIGNTQAVLDGDLDDFIEASLKQGV"
     gene            4360..>4671
                     /locus_tag="VLN25_11075"
     CDS             4360..>4671
                     /locus_tag="VLN25_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11132.1"
                     /translation="MPRPAIDQPELLALTEGVASARIVLRCYRFGDGAALFDALAAHR
                     EELMQWMEWPRRHQRVEDSESYARRMHAEFMLRRSMPMAIFDADSNRYLGGAGFHVPD
                     WN"
BASE COUNT          826 a         1591 c         1543 g          711 t
ORIGIN      
        1 acatggaata gcgcgcggcc cgtgggcggc gtgaccccgg ccacctcact gatggagctc
       61 caatgatgaa gcgcagtctc gtcgtgaccc tggcggccgt tgccgcgttg ataggatcga
      121 atgcgctcgc gcagcccgtc accgcgcgcg tgacgatgga gggaacgacg attgccgtcc
      181 agcccgaccc gatccggatc acgaaggcac agggcgcggt cgtgatccgc tgggagctgc
      241 ctgcgaatgc gaactacagc ttcgatccgc aggggatcgt gatcaacggt gaaagggtcg
      301 caggccagtt gcggccgcaa aaccagatcg cccactgtgg cggcggtccc aggcatatca
      361 cctgcaccaa cagcaacagc cgccgcggaa ccttcaagta cacggtgcga ctgctcgacg
      421 ccaaccggcg gctcgtcgaa aaggatcctg aaatcgtcaa tctcgagtga cgctcgtcgg
      481 cgcggacgcg atggcgtttg gcgatagcag ctcggcggcg cgccgcaccg cttcgttgta
      541 gcccagtccc ttcccttcgg acagcatatc cgccgccgca tcaccgagcg cccgctgcga
      601 ctgctcgatc aaaggcagca gaaaggcact gtcggcgctc ccgcgtttca ggcccgagcg
      661 ctccgcctgc gcctcggccg ctccgaacat cttcaacgcc gcgtcatgcc ggccgtccgc
      721 cgcgcacagt ccagcgagga tgtcgagcgt cgactggccg agctgccgtg accctaactc
      781 ggtcgccacc tgcagcgcct ggccgagcag cgcatgggcc gtgtcggcct gcccgcgctc
      841 gatggccacg acggcaaggt tcagccggcc gattgccacg ctctcgtggt cacccagtcc
      901 caggaggatc tgcaccgcct ccgcgtagag cgacttggca ccggccagat ccccctgctg
      961 acggagcagc tgcgcgtggc tgttcaacgc cgacgcgagc tggcgcggtt tgccgacggc
     1021 gcgcgcctcg atgatcgact ccgccgagta gcgttcggcg ttcgtcaggt caccttcgcc
     1081 gcggcacgcc tgcccgagcg tctgcagtac cgcggcgatg cggttctggt cgcccatttc
     1141 acgtgccagc acgaggcttt cctcgaggaa gccgcgcgct gcggcgtagc ggcccatgaa
     1201 agtgcagaac tggccgacgt cggccagccc gcgacagcgc gcgaggctgc gatcgtcagc
     1261 gcgcgtccgc gcgagcgcct cggccgtgac gcggtgaccc agtgtcagca ggccatggtg
     1321 gtaccagtag agcttcagca ggaagaccag ctgcaggccc tgttcgacgc cgccctcggc
     1381 acggtcgcac cagcgatgcg cgccgagcag gttctcgcgt tcacgatcaa gttccgcgat
     1441 ccagcggccc tgctcaggac cggccagtcc cgcccgggca tgctcggcga gcgccatgca
     1501 ccaggccaga tggagcgagc gcgtggccgc ttcatcgcca ctgcgcgtga gctgctctgc
     1561 ggcgtagtga cgcacggtgt cgagcatccg atagcgaccg ctatcgacat cgagcaccac
     1621 gagcgacttc tcgaccaggt tggccagcag gtccatcacg tcgccggcgg tgatgaagtc
     1681 acccacgccg accgactcgg ccgcttgcag agtccagccg ccgacaaaca ccgacagccg
     1741 ctggaagagc cgctgctcga gcgggggcag caggtcgtag gaccagtcga tcaacgcccg
     1801 cagcgtctgc tgccgcggca acacggtgcg atccgtcgtc accagcagct tgaagcgatc
     1861 ctgcaggcgc gcggcaatga cgtcgatcgg catggcccgc gtccgcgccg cggcgagttc
     1921 cagcgcaaga gggatgccgt cgagtcggcg gcagatctcc gcaactgccg cggccgcctt
     1981 cccggtcagc ctgaatgccg gctgcaccgc ggtggcccga tcgacgaaca accgtaccgc
     2041 gtcgttggcg agcagggatt cgacggagtg ttcggcctgg cccgggggcg gcaagggcgt
     2101 gacctggaac acggcctccc cggcgatgcg caacgcgtcc cggctggacg cgagcaccct
     2161 cacctgcggc cccgcctgca gcagccgctt cgccagctct gcgcatgcgt gcaccacatg
     2221 ctcgcagttg tccagcacga tgaggagctt ccggtcgcgc acgaagcggg tcagcgcatc
     2281 gagtgcggtg ccgccaggct cctccttgac cccaaggacg gaggcggcgg cctgcggcac
     2341 catctgcggc tcgaggatcg ccgccagttc gacgaaccag acgccgtccg gatagtcctc
     2401 gagcacgatc gcagctacct gcatcgacag ccgcgacttg ccgaggccac ccatcccgag
     2461 cagcgtcagc agccgactgt tggcgagcag gtgtcgcacc tgttccatct cgcgatcgcg
     2521 gccgacgaag gcgttcagct gttgcgcgag gttgttgggc gtgctcgcca tcgagcgcag
     2581 cgccggaaaa tcggtgcgca gctgcgggtg cacgagctgg aacagctgct cggcgctgcc
     2641 aaggtcgcgc agccgcacgg ctccaaggtc gcgcagaaca acgttaggcg gcaggcaccc
     2701 ggcgagtcga tcggccaccg cccgcgacag cagcacctgt ccaccgtggg ctgcgcacat
     2761 gatgcgtgcg gcccggttga cggcggggcc gtagaagtcg ttgtcgcgcc gctggtcggc
     2821 gcccaggtgc aggccgcagc gaacgctgag cgacgcgaga tcggccggcg gcttcgccag
     2881 cgccagctgc agctcgatca ctgccgcgag cgcgtcgctg gagtcgtcaa aggcggcgtg
     2941 gagtccgtct ccggtcatct tgacgacggt gccccgatgg ccctcgaccg cggcgcgaga
     3001 gacctcgtcg tgccacgcca acgtgcgcgc ggcccgaccc gggtcggatt cccataggcg
     3061 cgtgctgccc tcgatatccg agaacaggta ggtgactgtc gtggcagtcg actcgatcat
     3121 cggtccgcgg gttgctcggt cgttactagg gcaggcgcgc gcagtttacg tcggcacagc
     3181 cccagccttc ggtcggcacc gacggctggc ggaccgagtt cctataatcg cggactttcg
     3241 acatcgagat gctccatgga cgccgaacac ctgaaccaga tcgaaggcac catcgcggac
     3301 ctgcgcggtc gcctggccga actgcggagg tatctttgac gtcgatgtga aggaaacccg
     3361 gctggaggag atcaatcgcg cactcgagga cccgaaggtc tgggacgact cgaaacgcgc
     3421 gcaggagttc ggccgcgaaa agaagtcgct cgacctgatc gtcgtgaaga tgcgcacgct
     3481 gaccgatggc gtgcgcgacg ccggggaact gttcgagatg gcgcgcagcg atgacgatga
     3541 cgccacgctg cgatcggtcg atgccgacgt gacaacgctc gccaagcagg tggcggacct
     3601 cgagttccag cggatgttca acaatccgct cgaccccaat aactgcttca tcgagattca
     3661 ggcgggcgcg ggcggcaccg aggctcagga ctgggccggc atgctcgagc gcatgtacct
     3721 gcgctacgcc gagcggcgcg gattcaagcc cgagctgctg gacgaatcgc agggcgaagt
     3781 cgccggcatc aagggcgcca cgatcaagat cagcggcgac tatgcctacg gcatgctccg
     3841 caccgaaacg ggaatccacc ggctggtgcg caagtcgcct ttcgattcga atgcgcgccg
     3901 ccacacctcg tttgccagcg tggtcgtcta ccccgaggtc gatgagacag tcgacgtcga
     3961 gatcaatccg gccgacctgc gcatcgatgt gtaccgggcc tccggcgccg gcggccagca
     4021 cgtcaacaag accgaatcgg cagtgcgcat tacgcacgcc ccgtccggca tcgtcgtgca
     4081 gagccagaac gatcgctcgc agcatcgcaa ccgggccgaa tgcatggcga tgctgaagtc
     4141 gcgcttgtac gaactcgagc tgcgcaagcg gatggcggaa cagaacaagc tggaggacgc
     4201 caagaccgac atcggctggg gccaccagat ccgttcctac gtgctcgatc agtcgcgcat
     4261 caaggacctg cgcaccaacg tcgagatcgg caacacgcag gccgtgctcg acggagacct
     4321 cgacgacttc atcgaagcca gcctcaagca gggcgtctga tgccacggcc cgctattgac
     4381 cagccggagt tgctagcgct caccgaaggt gtggcgagcg cccgcatcgt gctgcgttgc
     4441 tatcgcttcg gcgacggtgc ggcgctcttc gatgcgctcg ccgcgcaccg cgaagagctg
     4501 atgcagtgga tggagtggcc gcggcgccat cagcgcgtcg aggactccga gtcgtatgcc
     4561 cggcgcatgc acgccgagtt catgctcagg cgatcaatgc cgatggcgat tttcgacgcc
     4621 gacagcaatc gctatctggg cggcgccggc ttccatgtgc ctgactggaa c
//
LOCUS       DATIRT010000483         1800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_773583, whole genome shotgun sequence.
ACCESSION   DATIRT010000483 DATIRT010000000
VERSION     DATIRT010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773583"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VLN25_11080"
     CDS             complement(<1..307)
                     /locus_tag="VLN25_11080"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HSQ11133.1"
                     /translation="MGRGRELLQGLMSFEKGKIVTAREAVQLIHDGDTVATSGFVGIG
                     FPENIAVALEQRFVESSEKDPDGIGRPEGLTLVYAAGQGDGKERGLNHLGHPGLVRRV
                     "
     gene            493..936
                     /locus_tag="VLN25_11085"
     CDS             493..936
                     /locus_tag="VLN25_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11134.1"
                     /translation="MALLQSLGVGTLVALLAPGALAQVKVQAIPDSVLRPYSVHHTGT
                     APAVAASLPAAPATAPSASMQLRDYSWLGGAVQVEASRANIDGSYKRPKVIVGLPSES
                     MKSWLYSAGFPTEQCLLPMVRARARINNDGEASGALWLYARCTFQ"
     gene            1011..1775
                     /locus_tag="VLN25_11090"
     CDS             1011..1775
                     /locus_tag="VLN25_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HSQ11135.1"
                     /translation="MQISNHVFIVTGGASGLGGATTRMLAAQGGKVVIADVQADRGEA
                     LARELGAHARYVKCDVSSEADGRAAVDAALALGPLRGLVNCAGIAIGEKVVGKEGPHS
                     LASFARVININLIGTFNMIRLAADAMSKQEPTAEGERGVLVNTASVAAFDGQIGQAAY
                     SASKGGVVGMTLPIARDLSRSGIRCVTIAPGLFATPMLLGMPQDVQDSLGRQVPFPSR
                     LGKPDEYAALVRHIVENVMLNGETIRLDGAIRMQPK"
BASE COUNT          311 a          599 c          567 g          323 t
ORIGIN      
        1 tcacacgacg taccagaccc gggtggccaa ggtgattgag accgcgctcc ttgccgtcgc
       61 cctgcccggc cgcatagacg agcgtgagcc cctcaggccg tccaatgcca tccggatcct
      121 tttcgctcga ctcgacaaag cgctgctcga gcgcaacggc gatgttctcc gggaaaccga
      181 tgccgacaaa gccactggtg gcgaccgtat cgccgtcatg gatcagctgc accgcttcgc
      241 gtgcggtgac gatcttgcct ttctcgaagc tcatgagccc ctgcagcagc tcgcgcccac
      301 gccccatcgc cgactcccct gacgttcgac cggcctctga tcggttgttc tgggccgggg
      361 agccgactgt agtcatccga ttggactctt gtcacgaaac tcctgcttcc ggcacagtaa
      421 tcttcacttg tgggcgtcgt ctcgcgaagg tacggtggcg caatgcacct gaacccgaag
      481 cgtttttcga ggatggcact cctgcaatcg ctgggggtcg ggacccttgt tgccttgctc
      541 gcccccggtg cgctggcaca ggtgaaagtc caggcaattc cggactccgt gcttcgcccc
      601 tattccgtgc accacacagg cacggctccg gcggtggccg cttcgcttcc cgcggcaccc
      661 gcgactgcac catccgcttc tatgcagttg cgcgattaca gctggctcgg gggcgccgtg
      721 caggtcgaag cctcacgagc gaatatcgac ggctcgtaca agcgaccgaa agtcatcgtc
      781 ggactgccgt cggaatcgat gaagagctgg ctgtactccg ccggcttccc gacagagcag
      841 tgcctgctgc ccatggtacg tgcccgtgcc cgcatcaaca atgacggcga ggcgagcggc
      901 gcgctctggc tgtacgcccg ctgcacgttc cagtagcgac ggctcggccg ccgtcgaggg
      961 ctcctctaca ctcccccggc tcgcactggg gcagttgaag gggacccgac atgcagatca
     1021 gcaatcacgt ctttatcgtc accggcgggg cgtccggact cggcggcgcg accacgcgga
     1081 tgctggcggc acaaggaggc aaggtcgtca ttgccgacgt ccaggccgac cgcggcgaag
     1141 cgctggcgcg cgaactcggc gcacatgccc gctacgtcaa gtgtgacgtc tccagcgaag
     1201 cagacggacg tgccgcagtc gacgcggcgc tggcccttgg gccgttgcgg ggcctcgtca
     1261 actgcgccgg cattgcgatc ggggaaaagg tcgtcggcaa ggaaggtccg cattcgctgg
     1321 cgtcgtttgc gcgcgtcata aatatcaacc tgatcggaac cttcaacatg atccggctcg
     1381 ctgccgatgc catgtcaaag caggagccga cggccgaggg cgaacgcggc gtgctcgtca
     1441 acacagcctc ggtcgcggcc ttcgacggcc agatcggcca ggccgcgtat tccgcttcga
     1501 agggtggcgt cgtcggcatg acgctgccga tcgcgcgcga cctgtcccgc agtggcatcc
     1561 gctgcgtgac gattgcaccg ggactcttcg ccacgccgat gttgcttggc atgccgcagg
     1621 acgtacagga ttcgcttggc aggcaggtgc cgtttccctc gcgcctgggc aaaccggacg
     1681 agtatgcggc gctggtacgg cacatcgtcg aaaacgtcat gctgaacggt gagacgatcc
     1741 gcctcgacgg cgcgatccgc atgcagccga agtaaagcga ggcttttcgc aaagaggcga
//
LOCUS       DATIRT010000484         2767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1363900, whole genome shotgun sequence.
ACCESSION   DATIRT010000484 DATIRT010000000
VERSION     DATIRT010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363900"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VLN25_11095"
     CDS             complement(<1..440)
                     /locus_tag="VLN25_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HSQ11136.1"
                     /translation="MKPYDLPDQRGHFGPYGGVFVAETLMHALEELRDAYAKYQHDPE
                     FVAEFQRELKHYVGRPSPIYHAKRWSELLGGAQIYLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKPRVIAETGAGQHGVATATVAARYGMKCVVYMGSEDVGR"
     gene            complement(437..1069)
                     /locus_tag="VLN25_11100"
     CDS             complement(437..1069)
                     /locus_tag="VLN25_11100"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HSQ11137.1"
                     /translation="MRTRIKICGLTREADVAAAVAAGADAVGFVCYPKSPRHVTPARL
                     RALARGLPPFATPVLLFVNAAADLVEGAIAAVPDALLQFHGDEHEPACSRYGRPYLRA
                     IRMDAEVDLLDSERQFHSAAALLADAPSDAYGGSGSSFDWSRVPLRRAKALVLAGGLT
                     ADNVGDAIRHLRPYAVDVSSGVEDAPGIKSETHINRFIAAVRAADEGLTA"
     gene            complement(1032..1850)
                     /gene="truA"
                     /locus_tag="VLN25_11105"
     CDS             complement(1032..1850)
                     /gene="truA"
                     /locus_tag="VLN25_11105"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012365693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HSQ11138.1"
                     /translation="MRIALGIEYDGAGFNGWQTQPDGRSVQDALERALAALTGQTVST
                     ICAGRTDAGVHAIDQVVHFDTDVERPLTAWVRGTNRFLPSEVSVRWARVVTPDFHARF
                     GARRRSYDYWILNDPVRSPLAHARATWVFRPLDAQAMQRAADLLVGTHDFTSFRSAEC
                     QAASPIRELHELHVRRRGRWLHLRATANAFLHHMVRNIAGTLVAVGLGRQPPTWVAEV
                     LAARDRSQAAPTLGASGLYLSRVEYDARFDLPAPDGSTPHWFDAHADQDLWTDT"
     gene            complement(1885..>2767)
                     /locus_tag="VLN25_11110"
     CDS             complement(1885..>2767)
                     /locus_tag="VLN25_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HSQ11139.1"
                     /translation="GADSVMQSSSAPPLTKMVVDTRMTGADTTMSTGDTKMPADTKMS
                     GPMTTSAPTTQFQATLPGAQPTSGLTPPAPAPAAMADFSSLDFDLGPTKMNLEPPTKM
                     PTLDVPAAGSSEVDYGFKPTSPIGRTEPPALADLDIAMPTSPALQPTATGIGLIPDLD
                     LNIPSTRMPMPMPAAAEALSRPTVIGKKQPVTAEIGALPDEQAQRLTANTDQATVPLI
                     DFDLSAVSLNPTTKRGETEPGSALAQQMATKLDLARGYIDLGVKDGARELLDEVMRDG
                     TREQRQAAVELIKQIEG"
BASE COUNT          455 a          904 c          930 g          478 t
ORIGIN      
        1 tggcgtccga cgtcctcgct gcccatgtac acgacgcact tcatgccgta acgcgcggcc
       61 acggtggcgg tcgctacgcc gtgctgcccc gcaccggtct ccgcaatcac acggggcttg
      121 cccatccgct tcgccagcag ggcctggccg atggtgttgt tcaccttatg tgccccggtg
      181 tgattcaggt cttcgcgctt caggtagatc tgtgcaccgc ccagcaactc gctccagcgc
      241 tttgcgtgat agatcgggct tggacggccg acgtagtgct tgagctcgcg ctggaactcg
      301 gcgacgaact ccggatcgtg ctgatacttc gcgtacgcat cgcgcagttc ctccagcgcg
      361 tgcatcagcg tctcggcgac gaacacgcca ccgtaaggac caaagtggcc gcgttgatcg
      421 ggcaggtcgt aaggcttcat gcagtcaggc cttcgtccgc ggcgcgcacg gccgcgatga
      481 accggttaat gtgtgtttcg ctcttgatac ccggcgcgtc ttcgaccccg ctgctgacgt
      541 caacggcata gggccgcagg tgtcggatgg cgtcgccgac attgtcggca gtcaggccgc
      601 ccgccagcac cagggccttc gctctccgca gcggcacgcg ggaccagtca aagctgctgc
      661 cagagccgcc ataagcgtcg gacggcgcat cggcaagcag ggctgccgcc gagtgaaact
      721 gacgctccga gtctaacaaa tcaacctcgg catccattcg gatcgcacgc agataaggcc
      781 gaccgtagcg gctgcaggcc ggctcgtgtt cgtcaccatg aaattgcagc agcgcgtccg
      841 gcacggcggc gatggcgccc tcgaccagat cggcggccgc attaacgaac aacaggaccg
      901 gagttgcaaa cggcggtagt ccgcgtgcga gcgcgcgcag acgggccggt gtcacatgac
      961 gtgggctttt cggataacag acgaagccga cagcatccgc acccgcagcc accgcggcgg
     1021 ctacatccgc ttcacgtgtc agtccacaga tcttgatccg cgtgcgcatc gaaccagtgc
     1081 ggcgtcgaac cgtccggagc cggtaaatcg aatcgggcat cgtactcgac ccgcgaaaga
     1141 tagagacccg atgctccgag tgtcggagcc gcctgcgagc gatcgcgcgc ggcgagcacc
     1201 tcagccaccc aggtgggagg ctgccggccc agcccgactg ccacgagcgt cccggcgatg
     1261 ttgcgcacca tgtgatgcag gaatgcattg gcggtcgccc tcagatgcag ccaccgcccg
     1321 cgtcgccgca cgtgaagttc gtgcagttcc cggattggcg atgccgcctg acactccgcc
     1381 gaacggaatg acgtgaagtc gtgcgttccg accagcaggt ccgcggcgcg ctgcatcgcc
     1441 tgcgcatcga gcggacggaa gacccaggtc gcgcgtgcgt gggcgagcgg agagcgcacg
     1501 ggatcgttga ggatccagta gtcgtagctg cgacgccgcg cgccaaagcg cgcatggaag
     1561 tcgggtgtga ccacccgagc ccatcgcacc gagacctccg acggaaggaa gcggttggtg
     1621 ccgcgcaccc acgcggtcag cggacgctcg acgtcggtat cgaagtggac gacctggtcg
     1681 atggcatgaa caccggcatc cgtgcgaccg gcgcagattg tggaaacggt ctgacccgtg
     1741 agcgctgcaa gcgcgcgctc gagtgcatcc tgcaccgagc gcccgtcggg ctgagtctgc
     1801 cagccgttga agccggcgcc gtcgtactcg atgccgagcg cgatgcgcac gggaatgatg
     1861 cagcggacat cgtgcccgct gcgttcagcc ttcgatctgc ttgatcagtt ccacggcggc
     1921 ctggcgctgc tcgcgcgtgc cgtctcgcat cacctcgtct agcagttcgc gcgcgccgtc
     1981 cttgacgccc aggtcgatgt agccgcgcgc cagatccagc ttggtggcca tctgctgcgc
     2041 tagcgcactg cccggctcgg tctcgccccg cttggtcgtc gggttgaggc tgacggccga
     2101 gaggtcgaag tcgatcagtg gcaccgtggc ctggtcagta ttagcggtga ggcgctgggc
     2161 ctgctcgtcc ggcaacgcgc cgatctcggc ggtcacgggc tgcttcttgc cgatgactgt
     2221 cggacgagac agcgcttccg ccgctgccgg cataggcatc ggcatccggg tcgacggaat
     2281 gtttagatcg aggtccggaa tcagcccgat accggtggcc gtcggctgca gtgccggcga
     2341 tgttggcatc gcgatgtcga gatccgccag cgccggaggt tctgtgcgcc cgatcgggct
     2401 ggtcggcttg aatccgtagt cgacctccga cgagccggcg gccgggacgt cgagcgtcgg
     2461 catcttcgtc ggcggctcga ggttcatttt ggtcgggccg aggtcgaaat cgagcgacga
     2521 gaagtcagcc atcgccgcag gcgcgggagc cggcggcgtc aggccggagg tcggttgcgc
     2581 acccggcaac gtggcctgga actgcgtagt cggcgccgag gtcgtcatcg gaccggacat
     2641 cttggtatcg gccggcatct tcgtgtcgcc ggtggacatc gtcgtatcgg cgcccgtcat
     2701 gcgggtgtcg acgaccatct tcgtcagcgg aggagcgctg ctgctctgca tgacggagtc
     2761 ggctccg
//
LOCUS       DATIRT010000485         6341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1758099, whole genome shotgun sequence.
ACCESSION   DATIRT010000485 DATIRT010000000
VERSION     DATIRT010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6341
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1758099"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1822
                     /gene="bamA"
                     /locus_tag="VLN25_11115"
     CDS             <1..1822
                     /gene="bamA"
                     /locus_tag="VLN25_11115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HSQ11140.1"
                     /translation="TGNKVFSDGTLRSQMDLSTPNWISWYTKRDQYSRQKLGADLESL
                     RSYYLNRGYLDFRIDSVNVSITPDKKDIAVTINVVEGEQYTVSGIQLGGEMLGLDEEL
                     APLITVKPGDVFNAEKVNAIGKAVTDRMSTLGYAFASANPVPDANREKREVAFTILVD
                     PGRRVYVRRVNIGGNVRTKDEVVRRELRQFEAGWFDAERVRLSKERLDRLGYFETVTI
                     DTPAVPTAPDQVDVNVTVKERATGNIQLGAGYSSSEGVVLTAGLSQQNLFGSGNALAF
                     EVNTSKSNTVLSITHTDPFVTTEGISRTIELYWRDSNLAELGLSSVGYATRGAGLVYG
                     VPFTEYDRVFLGLRYEGTTIDLTPTSPPRYIEYVQDFGETSDALALTAGWSSDSRDSL
                     LFPTRGRVQSAFGEFAIPVLDLEYYRLTYRLQQFFPIASFVTLAFGTELGYGNGYGGR
                     PYPFFKNFYVGGIGSVRGFDAASLGPRDIYGDPLGGTRRFNATFEALVPIPGANQAVR
                     AALFVDMGQVWGPGCNGIYSVPLNTNPQDVVDNPAAFCTGPYYGDSAGVDISLIRYSA
                     GIGISWLSPLGPLRLSYAYPLNSEPWDRIQRFQFQIGTGF"
     gene            1863..2366
                     /locus_tag="VLN25_11120"
     CDS             1863..2366
                     /locus_tag="VLN25_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HSQ11141.1"
                     /translation="MTQWLSAIAVAALLAAAPLAHAQELKVGVVNSDRILRDSQPARA
                     AMQKLEVEFSKRDKELQEMGARLKTSAERFEKDGPVMSESDRVRRQRELAEMDREFQR
                     KQREFREDFNQRRNEELQGLLERTNRIIRQIAEQEKFDLIVQEAVYFNPRIDMTERVL
                     RALNNAK"
     gene            2372..3448
                     /gene="lpxD"
                     /locus_tag="VLN25_11125"
     CDS             2372..3448
                     /gene="lpxD"
                     /locus_tag="VLN25_11125"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HSQ11142.1"
                     /translation="MTSGGLSLSELTAAIAGRTEGRLQATVRGDSAVRVAAVATLSVA
                     ATSDLSFLANSRYRSDAIGTRAGAIVLGAADAASLHAAGRQDLAMVVVDQPYAWFAFA
                     AQLLHPNEGRTPGIDPDASIAAGAEVDATATVAAGAVIGVGARVGPGAEVGAGVVLGP
                     GAVLGAGSQLHPRSVVLDRCEIGERCIVHSGAVVGADGFGFAPLDGRWIKIPQIGRVL
                     IGDDVEIGANTTIDRGTMGDTVIESGAKLDNQIQVGHNCVIGANTVIAGCVGIAGSAR
                     IGRNCQIGGAAMIAGHLSIADGCAIGPGTLVASSIGVPGHYTGFFPMMKHREWEKAAA
                     ILRQLDDLRDRIRELEAARRGDLK"
     gene            3445..3897
                     /gene="fabZ"
                     /locus_tag="VLN25_11130"
     CDS             3445..3897
                     /gene="fabZ"
                     /locus_tag="VLN25_11130"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HSQ11143.1"
                     /translation="MKLDINAILKLMPHRYPILLVDRVLELEPGARITAIKNVSINEP
                     FFQGHFPGHPVMPGVLIIEALAQAAAVLTYITMKTSYPEGTLFYFAGIDGARFKRPVV
                     PGDQLRLEVSMDRVKRGVGKFTGRALVDGDVVCETEMTCALRSEREAP"
     gene            3897..4685
                     /gene="lpxA"
                     /locus_tag="VLN25_11135"
     CDS             3897..4685
                     /gene="lpxA"
                     /locus_tag="VLN25_11135"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012357549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HSQ11144.1"
                     /translation="MPTVHATAVVDRAAELADDVEVGAYCVIGPKVKIGAGTRLGPHV
                     VVDGRTTIGRNNRFYPFCSIGGAPQDKKYGGEDTELAIGDGNTVRECCTVSLGTVQGG
                     GTTRVGSDNWIMAYAHIAHDCILGDHIILANNATLAGHVTLGDWAIMGGMSAIHQFCS
                     MGAHSMAAGGAIVLRDLPPFVICSGNPAEPHGINTEGLKRRGFATETINALRRAYRTL
                     YKDGLTTAEAIEAFESPSAEETDAGRAVAQLRDFVQNSQRGIVR"
     gene            4685..5830
                     /gene="lpxB"
                     /locus_tag="VLN25_11140"
     CDS             4685..5830
                     /gene="lpxB"
                     /locus_tag="VLN25_11140"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HSQ11145.1"
                     /translation="MPRPTCAFVAGEASGDLLAAGVIAELQRLAPGIQCAGVGGDRMI
                     EVGFDAWAHVRELSVRGYVEVLRHLPRLLKLRRDLAARLSAEPPAVFVGVDAPDFNLG
                     LEEKLRVRGIRVVHFISPSIWAWRAERIEQIRRAVDRMLLVFPFEQAIYDAANIPATY
                     VGHPLASMIPMTPDAPAARARLGLPADAPVVAVLPGSRADEVKYLGPTFLAALEHLLR
                     REPGIRFVLPVADAPLRALLESMLVAHAALVPAMMLVDGRSHDCLEAADTVLVASGTA
                     TLEAALFKRPMVIAYKMPAVSAWLMRRKKMIPYVGLPNIMAGEFLVPELLQEDATPVA
                     LAEALHAQLHDAPLRARLEQRFGAMHVELKRDTSALAAQAIIEVTRR"
     gene            5827..>6341
                     /locus_tag="VLN25_11145"
     CDS             5827..>6341
                     /locus_tag="VLN25_11145"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HSQ11146.1"
                     /translation="MRSRRATCALSLFEQAAQQDGRVCGVDEAGRGPLAGPVFAAAVI
                     LDPSRRIDGLADSKVLSPARRGELALLIREHSMAWAVAEASVGEIDALNILQATLLAM
                     RRAVELLALPAEYALIDGNQMPQLAIPGHAIVAGDATQPSISAASILAKTARDALMCR
                     LDAKHPGYGFSR"
BASE COUNT         1098 a         1998 c         2099 g         1146 t
ORIGIN      
        1 caccggcaac aaggtattca gcgatggcac cttgcggtcg cagatggacc tgtcgacgcc
       61 gaactggatc tcctggtaca ccaagcgcga ccagtattcg cgtcagaagc tcggtgccga
      121 cctcgaatcg ctgcgttcgt attacctgaa ccggggctac ctcgatttcc gcatcgactc
      181 ggtcaacgtc tcgatcacgc cggacaagaa ggacattgcg gttacgatca acgtcgtcga
      241 gggcgagcag tacacggttt cgggtatcca gctcgggggc gagatgctgg gactcgacga
      301 ggagctcgct ccgctgatca ccgtcaagcc gggcgatgta ttcaacgccg agaaggtcaa
      361 tgcgatcggc aaggcggtca ctgaccgcat gagcacgctc ggctacgcct ttgcgtccgc
      421 caatccggtg cccgatgcca atcgcgagaa gcgcgaagtg gccttcacca tccttgtgga
      481 tccgggccgc cgcgtgtatg tccgacgcgt caacatcggc ggcaacgtgc gcaccaagga
      541 cgaagtggtc cgacgcgaac tgcgccagtt cgaggccggc tggttcgatg ccgagcgcgt
      601 gcgcctgtcc aaggaacggc tcgatcgcct gggatacttc gaaacggtca cgatcgacac
      661 gccggccgtg ccgacggccc cggatcaggt cgatgtcaac gtgacggtca aggaacgggc
      721 gaccggaaac atccagctcg gtgccggcta ctccagctcc gaaggcgtgg tcctgacggc
      781 cggcctgtcg cagcagaacc tgttcgggtc gggcaacgcg ctggcgtttg aggtcaacac
      841 cagcaagtcg aacacggtct tgtcaatcac gcacaccgac ccctttgtaa cgacggaagg
      901 catcagccgc accatcgagc tgtactggcg cgattccaac ctggcagaac tgggattgtc
      961 gagcgtcggc tacgcgacgc gaggcgcggg gctcgtttac ggcgtgccgt tcaccgagta
     1021 cgaccgcgtc ttcctcggac ttcgttacga gggtacgacg atcgatctga cgccgacatc
     1081 gccgccgcgc tacatcgaat acgtacagga tttcggcgag acatcggatg cgctcgcact
     1141 gacggccggg tggtcgtcag acagtcgcga cagcctgctc ttcccgacgc gcggccgcgt
     1201 gcagagcgcc ttcggcgagt tcgcaattcc ggtgctggac cttgagtatt accgtcttac
     1261 gtaccgcctg cagcagttct tcccgatcgc gagcttcgtc acgctggcgt tcggcaccga
     1321 actcggctac ggcaacggct acggtggtcg gccttacccg ttcttcaaga acttctatgt
     1381 cggtggtatc ggcagtgtgc gtggcttcga cgccgcttcg ctgggcccgc gtgatatcta
     1441 tggtgatccg ctcggtggca cacgccggtt caacgcgacc ttcgaggcgc tggtgcctat
     1501 tcctggcgcc aaccaggcag tacgtgcagc cctgtttgtc gacatgggac aggtctgggg
     1561 gccgggctgc aatggcatct acagcgttcc tctgaatacc aacccgcaag acgtcgtcga
     1621 caatccggcc gccttctgca ccggaccgta ctacggcgac tcggccggcg ttgacatcag
     1681 cctgattcgt tactcggcgg gtatcggtat ttcctggctt tcgccgctcg gtccgctgcg
     1741 gctgtcttac gcgtatccgc tgaactccga gccgtgggat cgcatccagc gcttccagtt
     1801 ccagatcgga accggcttct gatgtggcaa aatcacttgg ttaaacgagt gcaggaagac
     1861 ctatgaccca gtggctttcg gcaatagccg tggccgcgct gctcgccgcg gcgccgctcg
     1921 cccatgcgca ggaactgaag gtcggcgtcg tcaactcgga ccgcatcctg cgcgactcgc
     1981 aaccggcgcg agctgcgatg cagaagctgg aagttgagtt ttccaagcgc gacaaggaac
     2041 tgcaggaaat gggagcgcgc cttaagacct cggccgagcg attcgagaag gatgggccgg
     2101 tcatgtcgga aagcgaccgc gtacgtcgtc agcgcgaact ggccgaaatg gaccgcgagt
     2161 ttcagcgcaa gcagcgcgaa ttccgggaag acttcaacca gcgccgcaac gaggaactgc
     2221 agggactgct ggaacgaacc aaccggatca tccgccagat cgccgagcag gagaagttcg
     2281 acctgatcgt ccaggaggcg gtctacttca atccgcgcat cgacatgacg gagcgagtgc
     2341 tgcgcgcgct caacaacgcg aagtaggccg catgacatcg ggcggactgt cgctgtcgga
     2401 gctgacagcc gccattgccg ggcgcactga gggtcggctg caggcaaccg tgcgagggga
     2461 ttccgcggtg cgtgtcgcag ccgttgccac gctgtccgtc gctgccacct ccgacctttc
     2521 gtttctcgcc aattcacgct accgcagcga tgccatcggc actcgggccg gcgccattgt
     2581 gctcggcgcc gccgacgccg cgagcctgca cgcagccggg cgccaggacc tcgccatggt
     2641 cgtcgtcgac cagccctatg cgtggttcgc attcgccgca cagttgctcc atccgaacga
     2701 ggggcgaacg ccaggcatcg atccggatgc cagcattgcg gcgggcgccg aggtcgacgc
     2761 gacagcgacc gttgccgccg gtgccgtgat cggcgtcggt gctcgggtgg gcccgggcgc
     2821 ggaggtcggc gccggggtag tcctcggtcc gggcgccgtc ctcggcgccg gttcgcaact
     2881 gcatccgcgc tcggtcgtgc tggaccgttg cgagattggc gaacggtgca tcgttcacag
     2941 tggcgcggtg gtcggcgccg acggcttcgg ctttgcgccg ctggacggcc gctggatcaa
     3001 gattccgcag atcggtcgcg tcctgatcgg cgacgatgtc gagatcggcg ccaacacgac
     3061 gatcgaccgg ggaacgatgg gagacaccgt catcgagtcc ggcgcgaagc tcgacaatca
     3121 gatccaggtc ggacacaact gcgtgatagg tgctaacacc gtgatcgcgg gttgtgtcgg
     3181 cattgcaggc agcgcccgga tcgggcgcaa ttgccagatc ggcggggcgg cgatgattgc
     3241 aggacacctt agcatcgccg acggctgcgc catcggaccc ggtacgctgg tggcgagttc
     3301 catcggggtg ccggggcact acaccggttt cttcccgatg atgaaacatc gcgaatggga
     3361 aaaggccgca gcgatcctcc ggcaacttga cgatttgcgg gaccgcattc gcgaactcga
     3421 agcggcgcgc cgcggagacc tgaaatgaag ctggacatca acgcaatcct gaaactgatg
     3481 ccgcaccggt atccgatcct tctcgtggat cgggtgctcg agctcgagcc tggcgcgcgc
     3541 atcaccgcca tcaaaaatgt gtcgatcaac gagccgttct ttcaggggca ctttccgggg
     3601 catcccgtca tgccgggcgt gctgataatc gaggcactcg cgcaggccgc agcggtgctg
     3661 acctacatca cgatgaaaac ctcctacccg gagggaacgc ttttctactt cgcgggcatc
     3721 gatggcgcgc gcttcaagcg accggtggtg cccggtgacc agttgcgcct ggaagtctcg
     3781 atggatcgtg tcaagcgggg cgtcggtaag ttcaccgggc gtgcgctggt cgacggcgat
     3841 gtcgtctgtg agactgagat gacgtgtgcc ttgcgcagcg aaagggaagc tccgtagtgc
     3901 ccaccgttca cgccacggcg gttgtcgacc gcgcggccga gctggccgac gatgttgagg
     3961 tcggtgcgta ctgtgtcatt ggcccgaagg tgaaaatcgg tgctggcacg cggctcggcc
     4021 cgcatgtcgt cgtcgacggt cgcacgacga tcggacgcaa caaccgcttc tacccgttct
     4081 gttcgatcgg cggcgcgccg caggacaaga aatacggcgg cgaggatacc gaactcgcta
     4141 tcggcgatgg caatacggtc cgtgagtgct gcacggtcag cctggggaca gtgcagggtg
     4201 gcggtaccac gcgcgtcggt tcggacaact ggatcatggc ctacgcgcac atcgcccacg
     4261 attgcatcct gggggatcac atcatcctgg ccaacaacgc cacgctggcc gggcacgtga
     4321 ccctcgggga ctgggcgatc atgggcggca tgtctgccat ccatcagttc tgctcgatgg
     4381 gcgcgcactc gatggccgca ggtggcgcca tcgtgctgag ggatctgccg ccgttcgtga
     4441 tctgcagcgg caatccggct gaaccccatg gcatcaatac cgagggcttg aagcggcgcg
     4501 gctttgcaac tgagacgatc aacgcgctgc gccgtgccta ccgcacgctg tacaaggacg
     4561 ggctgaccac ggcagaagcg atcgaggcgt tcgagtcacc ctccgccgag gaaacagatg
     4621 cgggccgtgc tgtcgctcag ttgcgggact tcgtgcagaa ctcccagcgc ggcatcgtgc
     4681 gttgatgccg cggccgacct gcgccttcgt ggccggggag gcatcgggcg acttgctcgc
     4741 ggccggagtc atcgccgaac tgcagcgact cgcaccggga attcagtgtg ccggcgttgg
     4801 cggcgatcgc atgatcgagg tcggcttcga cgcctgggcc cacgtgcgcg agctctcggt
     4861 acgcggctac gtcgaagtcc tgcgtcacct gccgcggttg ctgaagcttc gtcgcgatct
     4921 ggcggctcgt ctgagcgctg agccgccagc tgtgtttgtc ggcgtcgatg cgccggactt
     4981 caacctgggg ctggaagaaa aactgcgcgt gcgcggaatc cgcgtggttc atttcatcag
     5041 tccttcgatc tgggcgtggc gcgccgagcg catcgagcag attcgacgcg cggtggatcg
     5101 catgttgctg gtgttcccgt tcgagcaagc gatctacgat gcggcgaata ttccagcgac
     5161 ctacgtggga cacccgctgg ccagcatgat cccgatgaca cccgatgcac cggcggcgcg
     5221 cgcgcgtctc ggccttcccg ccgacgcgcc ggtggtcgcg gtgctgccgg gatcgagggc
     5281 cgacgaggtc aagtacctcg gaccaacctt tctcgcggcg ctggaacacc tgcttcgtcg
     5341 cgagcccggc atccgcttcg tgttgccggt ggcggatgcc ccgctgcggg cgctgctgga
     5401 gtcgatgctg gtcgcgcacg ctgcgctggt tcccgccatg atgctggtcg acgggcgctc
     5461 gcatgactgc ctcgaggccg ccgacaccgt actcgttgcg agcggcacgg cgacgctcga
     5521 ggcggcgcta ttcaagcggc cgatggtcat tgcctacaag atgccggccg tgtcggcctg
     5581 gctgatgcgt cgcaagaaga tgatcccgta cgtcggtctg ccgaacatca tggcgggtga
     5641 attcctggtc cccgagttgc tgcaggaaga cgcgacgccc gttgcgctcg cggaagcact
     5701 gcacgcccag ttgcatgatg ccccgctccg cgcccggctc gagcagcgct ttggcgccat
     5761 gcacgtcgag ctgaaacggg acacttccgc actggcggcg caggcgataa ttgaggtgac
     5821 gcggcgatga gaagccgtcg cgccacctgc gctctcagtt tgttcgaaca ggcggcccaa
     5881 caggacggtc gtgtctgcgg ggtcgacgag gcggggcgcg gcccgctggc cgggccggtg
     5941 ttcgcagcgg cagtgatcct tgatccgagt cgtcgaatcg acgggctggc ggactccaag
     6001 gtgctgagtc ccgcgcggcg tggggagctt gcgctgctga tccgggagca ttccatggcc
     6061 tgggccgtgg ccgaggcgag cgttggcgag atcgacgcgc tcaatatcct gcaagcgacg
     6121 ttgctggcga tgcgccgcgc tgtcgagtta ctcgcgttgc cggccgagta tgcgctcatt
     6181 gacggcaacc agatgccgca gctggccatc cccggccatg ccattgtcgc cggcgacgcg
     6241 acgcagccaa gcatttctgc ggcttcgata ctggccaaga ccgcgcgcga tgcgctgatg
     6301 tgcaggctcg acgcaaagca ccccggctat ggcttttccc g
//
LOCUS       DATIRT010000486         2429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1266935, whole genome shotgun sequence.
ACCESSION   DATIRT010000486 DATIRT010000000
VERSION     DATIRT010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266935"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VLN25_11150"
     CDS             <1..482
                     /locus_tag="VLN25_11150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSQ11147.1"
                     /translation="SIQSIYAHHVLHGLGTFETEPPGVEEMGSRHAQITAAGFPYLVA
                     VEGSHVIGYAYANHFRTRPAYRYTVEDSVYVRPDTHGRGVGKALLNELVTRCRSQGLQ
                     QILAVIGDSGNVASIGVHRACGFELVGVMKSVGRKFDRWVDVVVMQRALAPGPVDAD"
     gene            complement(498..>2429)
                     /locus_tag="VLN25_11155"
     CDS             complement(498..>2429)
                     /locus_tag="VLN25_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HSQ11148.1"
                     /translation="EAVQLIHDGDTVATSGFVGIGFPENIAVALEQRFVESSEKDPDG
                     IGRPEGLTLVYAAGQGDGKERGLNHLGHPGLVRRVIGGHWGLVPKLQALAVANRIEAY
                     NLPQGVITHLFRDIAAHKPGHLSRIGLGTFVDPRHGGGKINERTTEDVVELMTVGGEE
                     YLFYKAFPIHVGVVRGTTADPDGNITMEREALTLEALAIAMAARNSGGIVIVQVERIA
                     ERGSLNPRNVKIPGVLVDCIVVAEKPEYHMQTFVEQYNPGFSGEIRVPVSSLAPMPMS
                     ERKIIARRAAFELQANSVVNLGIGMPEGVSAVAAEEKVIDLLTLTAEPGVVGGIPASG
                     LNFGAAVNTQAIIDQPYQFDFYDGGGLDLAFLGLAQADREGNLNVSKFGPRLAGAGGF
                     INISQSAKRVVFVGTFTAGDLQVRIEDGRLLLLNDIGPKKFVEQVEHRTFSGSEARRR
                     GQPALYITERCVLRLGDDGLELTEVAPGIDIERDILARMDFRPHIPRDPIRMDMAIFG
                     TEPMNLRERMLAIPLAQRLSYDEEHNVLFLNFERLSVRTPADVAAVHDEIKRRLDALG
                     HKVYGIVNYDNFDLAPEVADEYAAMVRQLVDHYYFGVSRYTTSGFLRAKLGKALSQRG
                     VAPHIYESATDALKHVRDA"
BASE COUNT          408 a          811 c          769 g          441 t
ORIGIN      
        1 cgtcgatcca gtcgatctac gcacaccacg tcctgcacgg actcggcacc tttgaaacgg
       61 agccgccggg tgtggaagag atgggatcgc gccatgcgca gatcacggcg gccggctttc
      121 cctacctggt ggcggtcgaa ggcagccacg tcatcggcta cgcctacgcc aatcatttcc
      181 gcacccggcc tgcctatcga tacaccgtcg aggactcggt ttatgtcagg cccgacacgc
      241 atgggcgcgg cgtcggcaag gccttgctga acgaactcgt tacgcgttgc cgttcccagg
      301 gtttgcagca gattctcgcg gtcatcggcg actccggcaa tgtcgcctcg attggcgtgc
      361 accgtgcgtg cggcttcgag ctcgtcggcg tgatgaagtc ggtcggtcgc aagttcgacc
      421 gctgggtcga tgtggtcgtc atgcagcgcg cgctggcccc gggccccgtc gacgcagact
      481 gaggcgggca ttggcggtca ggcgtcgcgc acgtgtttca gggcgtcagt cgccgactcg
      541 tagatgtgcg gcgccacccc gcgctgtgac agcgccttgc cgagcttggc acgcaggaac
      601 ccagaggtcg tgtaacggct gacgccgaag tagtagtggt cgacgagctg ccggaccatc
      661 gccgcatact cgtccgccac ctcgggtgcc aggtcgaagt tgtcgtagtt cacgatgcca
      721 tagaccttgt gccccagcgc gtcgagccgg cgcttgatct cgtcgtgtac cgcggcgaca
      781 tcggccggag tccgcaccga caaccgctcg aagttgagga agagcacgtt gtgctcctcg
      841 tcatacgaca ggcgttgcgc gagcggaatc gccagcatgc gttcgcgcag gttcattggc
      901 tccgtgccga atatcgccat gtccatgcgg atgggatcgc gcgggatgtg cggcctgaag
      961 tccatccgcg caaggatgtc gcgctcgatg tcaatgcccg gtgccacctc ggttagttcg
     1021 aggccgtcgt caccgagtcg caggacgcag cgctccgtga tgtacagggc cggctggcca
     1081 cggcgccgtg cctcgctccc ggaaaacgtg cggtgctcga cctgctcgac gaacttcttc
     1141 ggcccgatgt cgttgagcaa gagcaggcgg ccgtcctcga tgcgcacttg caggtcgccc
     1201 gccgtgaacg ttccgacgaa caccacgcgc ttggccgact gcgagatgtt gatgaagccg
     1261 cctgctcccg ccagccgcgg tccgaacttg ctgacgttga gattgccctc acggtcggcc
     1321 tgcgccagtc cgaggaaggc caggtcgagt ccaccgccgt cgtagaagtc gaactggtag
     1381 ggctggtcga tgatcgcctg cgtattgacc gctgcgccga agttgagtcc cgacgcggga
     1441 atcccgccga ccacaccggg ttcggccgtc agcgtcagca ggtcaatgac cttctcctcg
     1501 gccgccacgg cagacacgcc ctccggcatg ccgataccca ggttgacgac gctgttggcc
     1561 tgcaactcga acgcagcacg gcgggcgatg atcttgcgct cgctcatcgg catcggcgcg
     1621 agcgagctga ccggcacgcg gatctcgccc gaaaagccgg ggttgtactg ctcgacgaaa
     1681 gtctgcatgt ggtactcggg cttctccgcc acgacgatgc agtcgaccag cacgccgggt
     1741 atcttcacgt tccgcggatt cagtgaaccc cgctcggcaa tgcgctcgac ctgcacgatg
     1801 acgatgccgc ccgagttgcg cgccgccatc gcgattgcca gggcctcgag cgtcaacgcc
     1861 tcacgctcca tcgtgatgtt gccgtccggg tcggccgtgg tgccacgcac gacgccgaca
     1921 tggatcggaa atgccttgta gaacagatac tcctcgccgc cgaccgtcat cagctcgacc
     1981 acatcctccg tggtccgctc gttgatcttg ccgccgccat ggcgcgggtc gacaaacgtg
     2041 cccaatccga tgcgggacag gtgacctggc ttgtgggccg cgatgtcgcg aaagaggtga
     2101 gtgatcaccc cttgcggcag gttgtaggcc tcgatgcggt tggcgaccgc tagcgcctgc
     2161 agcttcggca ccaaccccca gtgcccgccg atcacgcgac gtaccagacc cgggtggcca
     2221 aggtgattga gaccgcgctc cttgccatcg ccctgcccgg ccgcatagac gagcgtgagc
     2281 ccctcaggcc ggccaatgcc atccggatcc ttttcgctcg actcgacaaa gcgctgctcg
     2341 agcgcaacgg cgatgttctc cgggaaaccg atgccgacaa agccactggt ggcgaccgta
     2401 tcgccgtcat ggatcagctg caccgcttc
//
LOCUS       DATIRT010000487         1823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_381419, whole genome shotgun sequence.
ACCESSION   DATIRT010000487 DATIRT010000000
VERSION     DATIRT010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381419"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1093
                     /locus_tag="VLN25_11160"
     CDS             77..1093
                     /locus_tag="VLN25_11160"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSQ11149.1"
                     /translation="MNLLIIGGGVFLGQALLGAALESGHTVTVFNRGMSRQWWPPGVK
                     WVVGDRAEDLHLLMNKSWDAVIDTCGYRPQDVELSCAALFDSCERYVYISSVSAYASF
                     AKPPIRESDPTASILGIDTQSVNGNNYGPMKAECERTVARVFGARSILVRPGLIVGPD
                     DPTGRFSYWPWRIAAGGRVLAPGLPERPIQFIDARDLAAWILRLIAQRASGAFNGTGP
                     NEGATFVELLDTCRWICGEEVEVEWVDERFLEREGVQPWLELPLWIPSHDATTRGFHY
                     VDTTRAKASGLKTRPMAVTLNDILEAGIPVASDKRRSGKMTRQRERDLLSVWLSYRQG
                     MAVA"
     gene            1243..1569
                     /locus_tag="VLN25_11165"
     CDS             1243..1569
                     /locus_tag="VLN25_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11150.1"
                     /translation="MSGVIASALAWYLFVLIEPWIDLQRWQGDAPIIELDPLSTLAPR
                     LYDDADEVGPPASAARRDHGLGPGPVQLFAPAFTVFRCELRGSVTYADQPCERGGMRV
                     LRLPRN"
BASE COUNT          288 a          613 c          581 g          341 t
ORIGIN      
        1 gcacgtcgct ttggctggct gaacggtagt ccgaaaagct ggccgaatgc ggcgttgccc
       61 cctaagatcg gccgccatga acctgctaat catcggcggc ggcgtgtttc tggggcaggc
      121 attgctcggt gccgcactgg aaagcggcca cacagtcacg gtcttcaacc ggggcatgtc
      181 gcgccagtgg tggccgcccg gcgtgaagtg ggtcgttggt gatcgagccg aagacctgca
      241 cctgctgatg aacaagagct gggatgcggt gatcgacacc tgcggctacc gcccgcagga
      301 cgtcgaacta tcctgtgcgg cgcttttcga cagttgcgag cgctacgtct atatctcgag
      361 cgtctcggcg tacgcatcgt tcgccaaacc gccgatccgg gagagcgacc cgaccgcttc
      421 gatcctcggc atcgatactc agtccgtcaa cggcaataac tacgggccga tgaaggccga
      481 gtgcgagcgc acggtggcgc gcgtgttcgg tgcgcgctcg atcctggtgc gaccgggact
      541 catcgtgggt ccggacgatc cgaccggccg tttttcctac tggccctggc ggattgccgc
      601 cggcgggcgc gtcctcgcgc ccggcctgcc ggagcggccg atccagttca tcgacgcgcg
      661 cgacctcgcc gcctggatat tgcgactgat cgcgcagcgc gccagcggcg cgttcaatgg
      721 caccgggcct aacgaaggcg cgacgtttgt cgagctgctc gacacttgcc gctggatttg
      781 cggcgaggag gtcgaggtcg agtgggttga cgagcgcttc ctcgaacgcg aaggtgtcca
      841 gccgtggctc gagctgccgc tgtggatccc gtcacatgat gcgaccacgc gcggctttca
      901 ttacgtcgac acgacgcgtg cgaaggccag cggcctgaag acacgcccga tggccgtcac
      961 gctgaacgac atcctcgaag ctggcatccc ggtcgcgtcc gacaagcgcc gctcggggaa
     1021 gatgacgcgc cagcgcgagc gcgacctgct ttcggtgtgg ctctcgtacc gccagggcat
     1081 ggccgtcgcc tgataccgtc atccggtgat ccgccgcgca gcaggtgcgg ccatcatttt
     1141 ccgaatcggg gataatggcg cagcttccca attcgctctg tcatgcacgt cgccgcgccc
     1201 gaccctcgta ttgatctgtc tctccgattt ctgagcctgc tcgtgtccgg cgtcatcgcc
     1261 tcggcactgg cctggtacct cttcgtgctc atcgagccat ggatcgatct gcagcggtgg
     1321 cagggcgacg cgccgatcat cgagcttgat ccgctttcca cgctcgcgcc gcggctctac
     1381 gacgacgctg acgaagtcgg cccgcccgcg tcagcggcgc ggcgcgacca cggcttgggc
     1441 cccggaccgg tacaactgtt cgcgcccgcc ttcacggtgt tccgctgcga actccgagga
     1501 agcgtcacct acgcagatca gccctgcgaa cgcggtggca tgcgtgtgct acgcctgcct
     1561 cgcaactgac ccgccgttac aacttgaaac gactcccggt cgacgactga ccgacgtgca
     1621 ccgttgaacc tcctgtcaca ccaggagatc gaacgatgaa caaaagtctg ttagttgcca
     1681 ccctcgccgt tgccgccctc gcgccgcttg ccgcgcatgc cggcggtggc gtcaccgtca
     1741 gtgtcagcac gccggagttc ggtttccgca ttggcgcgcc gttctatggt gcgccgttcc
     1801 atggcgcacc ggtctacggg ccg
//
LOCUS       DATIRT010000488         2126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_183675, whole genome shotgun sequence.
ACCESSION   DATIRT010000488 DATIRT010000000
VERSION     DATIRT010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183675"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VLN25_11170"
     CDS             complement(<1..241)
                     /locus_tag="VLN25_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HSQ11151.1"
                     /translation="MSIAVQTVNGVARIAIARPEKKNAITVAMYQAMADAIGAAHDDG
                     SVRAILIHGEKDIFTAGNDLEDFMKNPPSGMDAPVF"
     gene            complement(255..734)
                     /locus_tag="VLN25_11175"
     CDS             complement(255..734)
                     /locus_tag="VLN25_11175"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HSQ11152.1"
                     /translation="MGVASRFYEAFMVRDFYTMGLLYANHATFRDPVFPRLTAQGARL
                     MWQMLLSEAEDLEVNVNILEDTPDRARVDWTARYTFTPTKRVVVNRVHTEMVIAAGKI
                     VQQVDDFSFWRWSGQALGWRGWLLGFTPIVRDKVQGKAAKSLKEFAQFVALTSREGK"
     gene            complement(742..1596)
                     /locus_tag="VLN25_11180"
     CDS             complement(742..1596)
                     /locus_tag="VLN25_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSQ11153.1"
                     /translation="MHKATVTTDDGFKLQATTFGDSQRCHAGVLIVPAMAVEQAYYEG
                     FARWLAERGYFAVTFDYRGIGRSRRGSLRQLEADVFTWARRDTEAMVEFVAGHLGDRP
                     LLWVGHSLGGQILGLLERHDRVAAMVTVAAGSGYWRDYVPGLRRLAPLLWFGIVPVLL
                     GVFGYFPGRRLGMIGDVPAGVMRQWRSWCLHPDYLFGIEDPELRVTYAGLRQPILSVS
                     FTDDEYMSARNIESLHAFYAGAPRAMRRIAPQEMGAKSIGHFGFFRRRHADSLWPVVG
                     DWLAGATR"
     gene            complement(1866..>2126)
                     /locus_tag="VLN25_11185"
     CDS             complement(1866..>2126)
                     /locus_tag="VLN25_11185"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011353151.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HSQ11154.1"
                     /translation="AEDMGWIELNEAFAAQAVAVINDLGLDRSKVNPMGGAIALGHPL
                     GATGAIRAATVVHALRRRNLKYGMVTMCVGTGMGAAGIFERV"
BASE COUNT          377 a          761 c          645 g          343 t
ORIGIN      
        1 ggaacaccgg cgcgtccatg cccgacggcg ggttcttcat gaagtcttcg aggtcgttgc
       61 cggccgtgaa gatgtccttc tcgccgtgga tcaggatggc gcgcacgctg ccatcgtcgt
      121 gggcggcacc aatggcgtcc gccatggcct ggtacatcgc gaccgtgatc gcattcttct
      181 tttccgggcg cgcgatcgcg atgcgcgcaa cgccgttgac cgtctggacc gcaatgctca
      241 ctggctctcc ccgttcattt tccctcgcgg cttgtcagcg caacgaactg cgcaaattcc
      301 ttcagcgact tggccgcctt gccctgcacc ttgtcacgca cgatcggcgt gaacccgagc
      361 agccacccgc gccaccccag cgcctggccc gaccagcgcc agaagctgaa gtcgtcgacc
      421 tgctgcacga tcttgccggc ggcaatcacc atttccgtgt ggaccctgtt gacgaccacg
      481 cgcttggtcg gcgtgaacgt gtagcgcgct gtccagtcga cacgcgcgcg gtccggtgtg
      541 tcctcgagga tgttgacgtt gacctccagg tcctcggcct ccgacagcag catctgccac
      601 atcagtcgcg ccccctgggc ggtcagccgc ggaaacaccg gatcacgaaa cgtcgcgtga
      661 ttcgcataga ggaggcccat ggtgtagaag tcccgcacca tgaacgcttc gtagaaacgc
      721 gaggcgacac ccatggcggc ctcagcgcgt cgcgccggca agccagtcgc cgacgaccgg
      781 ccagagcgag tcggcgtgac ggcggcggaa aaagccgaag tgcccgatgc ttttggcgcc
      841 catctcctgc ggcgcaatgc ggcgcattgc ccgcggagcg cccgcgtaaa aggcgtgcag
      901 tgactcgatg ttgcgtgccg acatatattc gtcgtccgtg aacgaaaccg agaggatagg
      961 ctgccgcagc ccagcgtagg tgacacgtaa ctccggatcc tcgatgccga acaggtaatc
     1021 cggatgcagg caccacgaac gccactgccg catcaccccg gcaggtacat cgccgatcat
     1081 gccgaggcgc cgacccggga aatacccgaa caccccgagc agcacaggca cgatcccgaa
     1141 ccacagcagc ggcgccaatc gtcgcagccc ggggacgtag tcgcgccaat agccgctgcc
     1201 cgcggccacg gtcaccatcg cggcgacacg gtcatgtcgc tccagcagcc cgaggatctg
     1261 gccgccgagg ctgtgcccca cccacagcaa cggtcggtct ccgaggtgtc cggcaacgaa
     1321 ctcgaccatg gcttccgtgt cacgtcgcgc ccaggtgaag acatcggcct cgagctgacg
     1381 caacgaaccc cggcgcgagc ggccgatacc gcgatagtcg aaggtgacgg cgaagtagcc
     1441 gcgctctgcc agccagcgcg cgaagccttc gtagtacgcc tgctcgaccg ccatcgccgg
     1501 gacgatgagc acgcctgcgt ggcatcgctg cgagtcgccg aaagtcgtcg cctgcagttt
     1561 gaagccgtcg tccgtcgtga ccgtcgcctt atgcatcgtc gcccagaaca acgctcctca
     1621 ccgaacttcg ctatccatgg tccacggcac gatgttagag ccgcggtaac tgaccgcacg
     1681 gcggcttcga cccgtgcggc gcagagcggc gcccgccgcg ccgcgcgcac tcacggtggc
     1741 cagcggagcg cagcgcagtc ggctaccggc ttcgacttcg tctgcctctg ccagcgaagt
     1801 cgaactgctc tctccgcttt cgatgcacgt gcgagcggcg gggccgctcg cgtacatcga
     1861 aagcgctaaa cccgctcgaa tatccctgcc gcccccatcc ccgtgccgac gcacatcgtc
     1921 accatgccgt acttgaggtt gcgacgacgc agcgcatgca cgacggtggc ggcacggatc
     1981 gcgccagtcg ctcccagcgg atggcccagc gcgatcgcac cgcccatcgg gttcaccttg
     2041 ctgcggtcga gcccgagatc gttgatcacg gccaccgcct gcgcggcgaa cgcctcgttc
     2101 agctcgatcc agcccatgtc ctcggc
//
LOCUS       DATIRT010000489         5171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1071000, whole genome shotgun sequence.
ACCESSION   DATIRT010000489 DATIRT010000000
VERSION     DATIRT010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5171
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071000"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VLN25_11190"
     CDS             <1..224
                     /locus_tag="VLN25_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HSQ11155.1"
                     /translation="GVGANAQLRERLQADAIRHGSTVFFPPLNLCTDNGAMIALAGLA
                     VLRRTDPATLGRRLGFAVHPRWTLAEATA"
     gene            complement(231..866)
                     /gene="plsY"
                     /locus_tag="VLN25_11195"
     CDS             complement(231..866)
                     /gene="plsY"
                     /locus_tag="VLN25_11195"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004522633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HSQ11156.1"
                     /translation="MQFLYTAVAILAAYLVGSVSFAVVVSRMLGLADPRTYGSKNPGA
                     TNVLRSGSRAAAVLTLVGDALKGWLAVWLVQRFGPAVGIGEWGVALAGIAVFLGHLYP
                     VYFGFKGGKGVATAAGVIVALNPWLALATGLTWLIIAFFFRYASLASMVAAVFAAFYS
                     AFGWGFDQRFVAVAVIAGFVIYRHQANIRNLMAGKERRIGQKSVSSPVHRS"
     gene            complement(870..1361)
                     /locus_tag="VLN25_11200"
     CDS             complement(870..1361)
                     /locus_tag="VLN25_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HSQ11157.1"
                     /translation="MGKTKHVSETPATHLLRDRGILFTEHTYEYVEHGGTAVSALALG
                     VEEHAVIKTLVMQDEEGAPLLVLMHGDCTVSTKNLARQINRKRIEPCRPDAAQRHTGY
                     LVGGTSPFGARKNMPVFVERTVLELPRILINGGRRGYLVGLAPALLTDLLKAIAVDCA
                     LKE"
     gene            complement(1420..2301)
                     /gene="xerD"
                     /locus_tag="VLN25_11205"
     CDS             complement(1420..2301)
                     /gene="xerD"
                     /locus_tag="VLN25_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HSQ11158.1"
                     /translation="MDPLVDRFLDTLWLEDGLARNTLAAYRQDLDGLSRGLTPRGATL
                     ATASEADLTSYMASQHAVTRATTANRRLAVLRRFYRWALRERLVPVDPTLRLQGAHRV
                     ARFPKTVSESQVESLLAAPDASNALGLRDRAMFELLYATGLRVSELVGLKLGELSLSE
                     GLVRVVGKGSKERIVPLGEEARAWIERYLKRARPELLAGRVSDAVFVTRRSGAMTRQM
                     FWILVKRYALLAGIHAPLSPHGLRHAFATHLLNHGADLRVVQLLLGHADISTTQIYTH
                     VARARLKTLHAEHHPRG"
     gene            complement(2310..2813)
                     /locus_tag="VLN25_11210"
     CDS             complement(2310..2813)
                     /locus_tag="VLN25_11210"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HSQ11159.1"
                     /translation="MMQQVATRIDRQVAVVAAPFGAVAVETDSGHVTALRFLPPTPEV
                     APSNDLGVEAVEQLKRYFADSGFRFDLPLDIRGSAFQCLVWDAISAIPSGTTRRYGEL
                     AADLAAPARAVGQACGDNRLPIVIPCHRVIGADSLGGFAHASSGFSLTVKRWLLEHEG
                     ALRGTLI"
     gene            complement(2816..3406)
                     /locus_tag="VLN25_11215"
     CDS             complement(2816..3406)
                     /locus_tag="VLN25_11215"
                     /inference="COORDINATES: protein motif:HMM:NF027363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HSQ11160.1"
                     /translation="MKNWLALTAAATTTALLAWGTPALAQPVEVEGQKFEPTVQVGAQ
                     TLTLNGVGLRKRAIFKVYVNGLYVPQKSTDAATLINEKGARRASLRMLRDVDADSFVS
                     AFSDGLKANLSEAQLTALKPQIDTFNATLKSIGEAKKGDVINFDYTPEGGTRITVNGQ
                     PKGAAIPGHDFYSAVMRIWLGDKPVDDGLKKGLLGG"
     gene            complement(3493..4095)
                     /locus_tag="VLN25_11220"
     CDS             complement(3493..4095)
                     /locus_tag="VLN25_11220"
                     /inference="COORDINATES: protein motif:HMM:NF027363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HSQ11161.1"
                     /translation="MSHGKFLRRVLAAVLIASIAWVSAGASAQVVMVEGARYESSVQL
                     AGERLLLNGVGVRKRFVFDIYAGGLYVSQRAARTEDLVRQPGPKRVALRFLRDVDGEL
                     FVTSLHNGLKANHSEAELEKWKSQVDVLTRTIQTIALARRGDAINFEYTPQDGTRVTV
                     NGVTRGPLIPGADFYAAVLRVWLGETPADAGLKKGMLGAG"
     gene            complement(4117..5067)
                     /gene="ispH"
                     /locus_tag="VLN25_11225"
                     /gene_synonym="lytB"
     CDS             complement(4117..5067)
                     /gene="ispH"
                     /locus_tag="VLN25_11225"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HSQ11162.1"
                     /translation="MEVLLAQPRGFCAGVDRAIEIVERALAIHGSPIYVRHEIVHNTY
                     VVDDLRRKGAVFVNEIDEVPAGGTVVFSAHGVSKIVQDEAAQRGLRIFDATCPLVTKV
                     HVEVAKMHAAGYEIVMIGHEGHPEVEGTMGQLAAGMHLVETVEDVARLQVRNADRLAY
                     VTQTTLSLDDAAAVIDALKRRFPTIAEPKKADICYATQNRQDAVKFMAPQVDIVIVVG
                     SPNSSNSNRLREVAERFGVPSYLVDTAEQLDPAWFQGRRRIGVTAGASAPEVLVDELL
                     AALKKMGAGSVRTLDGVTEDVVFPMPKGLSRAAVRPESAT"
BASE COUNT          940 a         1744 c         1582 g          905 t
ORIGIN      
        1 gcggggtggg cgccaacgcc caattgcgcg aacgactgca ggcggatgcg atacgccatg
       61 gcagcacagt gttcttccct ccgctcaatc tgtgcaccga caacggcgcg atgatcgcgc
      121 tcgccggcct ggccgtgctg cgtcgtacgg atccggccac actgggccgg cgactcggtt
      181 tcgcggtcca tccgcgctgg accctggccg aggccaccgc ctgaatcgct ctaggagcga
      241 tgcaccggtg aggacacgct cttctgtccg atgcgccgct ccttgcccgc catcaggttg
      301 cggatgttcg cctgatgccg gtaaatgacg aagccggcga tgacggcaac cgcgacgaag
      361 cgctgatcga agccccaacc gaaggccgag tagaacgcag cgaaaacggc ggccaccatc
      421 gacgcgagcg acgcgtagcg gaagaagaat gcaatgatca gccaggtgag gcccgtggcc
      481 agcgccagcc acgggttgag cgcgacgatg acgccggcgg ccgtcgcgac acccttgccc
      541 cccttgaaac cgaagtacac cggatacaga tgtccgagga acaccgcgat cccggcgagc
      601 gcgacgcccc attcaccgat cccgacggct ggaccgaagc gctgcaccag ccatacggca
      661 agccagccct tcagcgcatc gccgaccagc gtcagcacgg cggccgccct gctgcccgaa
      721 cgcaggacat tcgtcgcacc cgggttcttc gaaccgtagg tgcgcggatc cgccagcccc
      781 agcatccggc tcaccaccac ggcaaatgaa acggaaccca ccaggtacgc cgccagtatc
      841 gccaccgccg tgtaaagaaa ctgcatgcct cactccttca gtgcgcaatc gaccgctatc
      901 gccttcagca gatcagtcag cagcgccggc gcaagtccga caaggtagcc ccgacgcccg
      961 ccgttgatca ggatgcgcgg cagctcgagt acggtgcgct cgacgaatac cggcatgttc
     1021 ttgcgtgcac cgaacggcga ggtgccgccg accagatacc cggtatggcg ttgggccgcg
     1081 tccggtcggc acggctcgat gcgcttgcgg ttgatctgcc gtgccagatt cttcgtcgat
     1141 acggtgcagt caccatgcat caggacgagc agcggtgcgc cctcctcgtc ctgcatcacg
     1201 agcgtcttga ttaccgcgtg ctcttcgact ccgagcgcga gcgccgacac ggcggtgccg
     1261 ccatgctcga cgtactcgta ggtgtgttcc gtgaacagaa ttccacggtc acgcaacagg
     1321 tgcgtggcgg gagtctcgga aacgtgtttc gtcttaccca tgtgcttggt cttgaccgtg
     1381 gctcgcgatt ctagggtcga gcctctgcgg acaggtcgat cagccgcgcg gatggtgctc
     1441 ggcatgcagt gtcttgagcc gcgcccgcgc gacatgcgtg tagatctgcg tcgtcgagat
     1501 gtcggcatgg ccgagcagca gctgcacgac acgcagatcg gcaccgtgat tgagcaaatg
     1561 agtcgcaaac gcgtgtcgca gcccgtgtgg cgacaacggc gcgtgtatgc ctgccagcag
     1621 tgcatagcgc ttgacgagaa tccagaacat ctggcgtgtc atcgcaccgc tgcgccgggt
     1681 cacaaagacg gcgtcggaca ctcgacctgc cagcagttcg ggccgcgcgc gcttcaggta
     1741 gcgctcgatc cacgcccgtg cttcctcgcc caggggaacg atgcgctcct tcgatccctt
     1801 gccgacgacc cgcaccaatc cctcggacaa cgacagttcg ccgagtttca gtccgaccag
     1861 ttcggacacg cgtaatccgg tcgcatagag cagttcgaac atcgcgcgat cccgcaggcc
     1921 tagggcgttc gaggcatcgg gtgccgccag aagcgattcc acctgcgact cgctaacggt
     1981 ctttgggaaa cgggccaccc gatgcgcgcc ctgcaatcgc agggttggat cgacaggcac
     2041 gaggcgttcg cgtagcgccc aacgataaaa gcgacgcagt acggccagtc gtcgattggc
     2101 cgttgtggcg cgtgtcaccg catgctggct ggccatgtac gacgtgaggt cggcttcact
     2161 cgccgttgcg agcgtcgcgc cgcgcggggt cagcccgcgc gacaggccgt caagatcctg
     2221 tcgataggcg gcaagcgtgt tgcgcgccag tccgtcttca agccagagag tgtcgaggaa
     2281 acgatcgacc agcggatcca tgagcgacgc tagatcagcg tcccgcgcag cgcgccttcg
     2341 tgttccagca gccagcgctt gaccgtgagc gaaaacccgc tcgaggcatg cgcaaatcct
     2401 ccgaggctgt ccgcgccgat gacgcgatgg cacggaatga cgatcggcaa ccggttgtcg
     2461 ccgcaggcct gaccgaccgc tcgcgccggc gctgccaggt ccgcagcaag ttcaccgtac
     2521 cgccgtgtcg tacccgacgg aatggcgctg atcgcgtccc agacgaggca ctgaaaggca
     2581 ctgccccgaa tgtccagcgg aaggtcgaag cgaaacccgg aatcggcgaa gtaacgcttc
     2641 agctgttcga ctgcctcgac gccgaggtca ttcgacggtg cgacttccgg cgtcggcggc
     2701 aggaagcgca gcgcggtgac gtgccctgag tcggtctcga ccgccaccgc cccgaacggc
     2761 gcagcgacca ccgccacctg tcgatcgatg cgcgtggcca cctgctgcat catcgtcagc
     2821 ctcccagcag ccccttcttc agaccatcgt cgacgggctt gtcgcccagc cagatgcgca
     2881 tgacggcgga gtagaagtca tgtccgggga tcgccgcgcc cttgggctgg ccattgaccg
     2941 tgatgcgcgt gccgccctcc ggtgtgtagt cgaagttgat gacatcgcct ttcttggcct
     3001 cgccgatcga cttgagcgtg gcgttgaacg tatcgatctg cggcttcagc gccgtcagct
     3061 gcgcttcgct caggttggct ttcaggccat cggagaacgc gctgacaaag ctgtccgcgt
     3121 cgacatcgcg cagcatgcgc agcgaggccc ggcgcgcgcc tttctcgttg atcagtgtcg
     3181 ccgcatcggt gctcttctgg ggcacataca ggccattgac atagaccttg aaaatggcgc
     3241 gcttgcgcag accaaccccg ttcagggtca gcgtctgggc gccgacctgc acggtcggtt
     3301 cgaacttctg cccctcgact tcaacgggct gtgcgagtgc cggggtaccc catgcgagca
     3361 gcgccgtcgt cgtcgccgct gcggtcagtg caagccaatt cttcatctgc tgtctcctcg
     3421 ctgttcgcga catgttcagt tcaatcgtac cgcccgtcgg cacgttggcc gccttcgcgt
     3481 gcacgggtcc ggtcagccgg cgccgagcat tccctttttc agtccggcat ccgcgggcgt
     3541 ttctcccagc cagacccgca gcacggcagc atagaagtct gctcccggaa tcaacgggcc
     3601 acgcgtcacg ccgttgacgg tgacccgcgt tccgtcctgc ggcgtgtact cgaagttgat
     3661 ggcatcgccc cggcgcgcga gcgcaatcgt ctggatcgtg cgggtcagca catcgacctg
     3721 tgacttccac ttctccagtt cggcttccga atgattggcc ttcagtccgt tgtgcagcga
     3781 tgttacgaaa agctccccgt cgacatcgcg caggaagcgc agtgccacac gcttcggccc
     3841 aggctgcctg accagatcct cggtccgtgc cgctcgctgc gacacgtaca acccgccggc
     3901 gtagatgtcg aacacaaagc gcttccgcac ccccacgcca ttcagaagga ggcgctcgcc
     3961 ggccagttgc accgagcttt catagcgcgc accctcgacc atgacgacct gcgccgacgc
     4021 gccggctgac acccatgcga tcgatgcgat cagcacggca gccagcacgc ggcggaggaa
     4081 tttgccatgt gacatgttct gtctcaggtc gcgggctcag gtcgcggact caggtcttac
     4141 agcagcgcgg ctcagtccct tcggcatcgg aaacaccacg tcttccgtga ccccgtcgag
     4201 tgtgcgaaca ctgccggcgc ccatcttctt cagcgcagca agcagttcgt cgacgaggac
     4261 ctcgggcgca gaagcgccgg ccgtcacgcc gatgcggcga cgaccctgga accacgccgg
     4321 atccagttgc tcggctgtgt cgaccaggta ggacgggaca ccgaaacgct cggcaacttc
     4381 acgcagccgg ttcgaattcg aactgttcgg gctgccgacc acgatcacga tgtcgacctg
     4441 cggcgccatg aatttgacgg catcctggcg gttctgagtc gcatagcaga tgtctgcctt
     4501 cttcggctcg gcgatcgtcg ggaaacgacg cttcagcgca tcgatgacgg ccgcggcgtc
     4561 atcgagcgac aacgtcgtct gcgtgacata agccagcctg tcggcgttgc gcacctggag
     4621 tcgggcgacg tcctcgaccg tctcgaccag gtgcatgccg gctgccagct gtcccatggt
     4681 gccttcgact tcggggtggc cttcatggcc gatcatcacg atctcgtatc cggccgcgtg
     4741 catcttggcc acttcgacat gcaccttcgt gaccaacgga caggtggcat cgaagatgcg
     4801 caaaccgcgc tgcgcagcct cgtcctgcac gatcttggac acgccatgcg ccgaaaacac
     4861 gacggttccg ccagccggaa cttcgtcgat ctcattgacg aaaaccgcgc ccttgcggcg
     4921 cagatcgtcg acgacatacg tgttatgcac gatctcgtgt cgcacgtaga tgggcgaacc
     4981 gtggatcgcc agcgcgcgct cgacgatctc gatggcgcgg tcgactcccg cgcagaaacc
     5041 tcgcggctgt gcgagcagaa cttccatact cagagcactc caacgacgtg gaccgcaaag
     5101 cgaatcggca tccccgccag cggatggttg aagtcgacga caacatgctc gtcgtcgcgc
     5161 tgcttaagga c
//
LOCUS       DATIRT010000490         5306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1857339, whole genome shotgun sequence.
ACCESSION   DATIRT010000490 DATIRT010000000
VERSION     DATIRT010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1857339"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..874
                     /locus_tag="VLN25_11230"
     CDS             89..874
                     /locus_tag="VLN25_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HSQ11163.1"
                     /translation="MDFLPDLRDNHLLAALPDPELWRLAPRLHPVPLQPGQVLYESGS
                     TISHAYFPTTALISSLYEFDDGTSAETAITGNEGMIGIAVFMGGESRLGRAVVLTAGH
                     AFRLGAQALKDEFHRTGPLMHLLLHYTQALITQTAQTAVCNRHHSLDQRLCRWLLLSL
                     DRSQGDDFAITQQVIAHMLGVRRESVTEAAHKLQELGLVRCGRGHIVVLDRRGLEKRA
                     CECYSAVRKEYDRLLPDRRATQAAALQRATPVGALQPVCSLAH"
     gene            961..1119
                     /locus_tag="VLN25_11235"
     CDS             961..1119
                     /locus_tag="VLN25_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HSQ11164.1"
                     /translation="MSLGTILLIVLILILIGVIPTWPHSRSWGYGPSGALGVVVLIII
                     VLLVMGKL"
     gene            1353..1967
                     /locus_tag="VLN25_11240"
     CDS             1353..1967
                     /locus_tag="VLN25_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative oxidase"
                     /protein_id="HSQ11165.1"
                     /translation="MTDAPQLERHQIPLDLSDRVALAFTKALRFFADTFFARRYGHRA
                     VVLETIAAVPGMVGGALQHLRALRRLESDRGWIRTLLDEAENERIHLMTFIQIARPSS
                     FERLLILLAQGVFYNLFFLLYLISSKTAHRVVGYFEEEAVTSYTEYLAGVDKGTHPNV
                     PAPRIAIDYWKLAPDATLRDVIIAVRADEAHHRDVNHQFANELS"
     gene            complement(2179..2712)
                     /locus_tag="VLN25_11245"
     CDS             complement(2179..2712)
                     /locus_tag="VLN25_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11166.1"
                     /translation="MAQMVSTAPMSKANYTQAMKDADAQYKSAKSACASLSGNAKDIC
                     AAEATGRHNVAKADTEAAYKHTPKARESARVAHAQATYDVAIQKCDDQAGNTKDVCVK
                     EAKAALVKGKADAKVDRVTADTRKDAATTQADARKDATAEKREAEYKVAIEKCDALAG
                     AAKDACLSAAKSRYGKS"
     gene            complement(2931..3122)
                     /locus_tag="VLN25_11250"
     CDS             complement(2931..3122)
                     /locus_tag="VLN25_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HSQ11167.1"
                     /translation="MSMNRDQVKGRIKEAKGTVKEAAGKLVGNEKLEQKGKTQKVLGE
                     AQAKFGDVKKDVKDVIKSA"
     gene            complement(3376..3906)
                     /locus_tag="VLN25_11255"
     CDS             complement(3376..3906)
                     /locus_tag="VLN25_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11168.1"
                     /translation="MTNSTMELDKNKTVKPDPASGPLGTHPVGTGLGAVAGGAAAGAA
                     VASVAGPVGTVIGAAAGAVAGALAGKGIAKMVDPTAEEAYWRQNYSSRPYVTSGATFD
                     EYGPAYRYGVDNYGRFEGRSFEQAEPELMREWDGAKGTSKLTWENAKHATRDSWQRLS
                     DFVERATPGDSDRDGK"
     gene            4338..5219
                     /locus_tag="VLN25_11260"
     CDS             4338..5219
                     /locus_tag="VLN25_11260"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HSQ11169.1"
                     /translation="MQKLEHRKQIDGLRFFAFLPVFLVHSDVTTFWWGSYGVSLFFVI
                     SGFLITRILITFENRPRRQMLKSFYARRALRIFPAYYLVLGLAAATVGIAYLPAFALY
                     VMNWGIFFYTWIQPELLDKAWDPTRMYGIHFWSLCVEEQFYLVFPLLFSFLRTGRTRL
                     TWLLALWAASIGFRFLCAAFLPTAAYGLIPLVCGEYLVAGAVGAVVFADTSGTAERIR
                     AAARQWMFPVGLALVVALFALYGPETPSIDHVMHPPHMQTLLALGFLAMVLGLWQTDN
                     RWLLGFFNYGPFRQAAT"
BASE COUNT          906 a         1675 c         1561 g         1164 t
ORIGIN      
        1 gtgcacctcg gttgcgcgac gtcaacggca gtgccgcgct aaggcctaaa aggagagaat
       61 ggtttcccca cgcaccccgg cgggtactgt ggatttcctc ccagatctca gagataacca
      121 tctgcttgcc gcgctgccgg accccgaact ctggcgcctg gcgccgcgac tgcacccggt
      181 cccgctgcag cctggacagg tcctgtacga gtcgggcagc acgatttcgc acgcgtactt
      241 tccgaccacc gccctcattt caagcttgta cgagtttgac gatggcacct cggcggaaac
      301 cgcgattacg ggtaacgagg gcatgatcgg catcgccgtg ttcatggggg gcgaatccag
      361 gctcggtcga gccgtggtgc tgaccgccgg gcacgccttt cggctgggag cacaggctct
      421 gaaagacgag ttccaccgga ccggcccgtt gatgcacctg cttttgcact acacacaggc
      481 actgatcacg cagacggcgc agacggcggt gtgcaatcga caccattcac tggaccagcg
      541 actgtgccgc tggctgctgt tgagcctgga tcgctcgcaa ggcgacgact tcgcgatcac
      601 gcagcaagtg atagcccaca tgctcggggt gcgacgcgaa agcgtgactg aggcagctca
      661 caagttgcag gaactcggtc tcgttcgatg cgggcgtggc catatagtgg tcctggatcg
      721 caggggcctg gaaaagcgcg cgtgcgagtg ttactcggcc gttcggaagg aatatgaccg
      781 cctcctgccc gatcggcggg cgactcaggc ggcggcgctc cagcgagcta ctcccgtcgg
      841 cgcgctacag ccggtatgtt cgctagcgca ctgaccggtc taaagcgaac ggctaccgta
      901 tatctgtgcc agttcggcac gggaattgcg tgcgcgtgct gaccacttcg aggaaaaacc
      961 atgagcttgg gaaccatcct gttgatcgtc ctgatcctga tactgatcgg cgtcatcccg
     1021 acctggccgc acagccgaag ctggggctac gggcctagcg gcgcgttagg cgtcgtggtg
     1081 ctcatcatca tcgttctgct ggtgatggga aagctttgac cggtcgttcc ccgcctgtca
     1141 tccggcgggt tgcaactgcg accagacgca cggtcgagaa cggactgtcg actgggcggg
     1201 tgagtggcac tgtgagctga aagtgagcgt tacatacgga ggcgcgctgg ctgcggcgct
     1261 cgcgtcagca gccgaccgcc gcccgctcgt tgttcgcgtc tctgaactgt ttgctcgagt
     1321 ctggaagtac tggctgtgac tgccccctca cgatgactga tgcccctcag ctcgagcgac
     1381 accagattcc gctggatttg tcggatcgcg ttgcacttgc cttcacgaag gcactgcgat
     1441 tcttcgctga cacgttcttc gcgcgtcggt atggccatcg agcggttgtg ctcgaaacga
     1501 ttgctgccgt gcctggcatg gtcggcggtg cccttcagca cttgcgcgcc ttgcgtcgac
     1561 tggaaagcga ccgaggctgg atccggacgc tgcttgacga ggcggagaat gaacgaatac
     1621 acctgatgac gttcattcag attgcgcgtc cttcatcgtt tgagcggcta ctcattctgc
     1681 tggcgcaggg cgttttttat aaccttttct tcctgcttta tctgatctcc tccaagaccg
     1741 cgcatcgcgt cgtgggctac ttcgaagagg aagcggtcac cagctacacg gagtacctcg
     1801 ctggagttga caaaggcacc caccccaacg ttccggcgcc tcggatcgcc attgactact
     1861 ggaagctcgc gcccgacgct acattgcgtg atgtcatcat cgcagtccgt gccgacgagg
     1921 cccatcaccg ggacgtcaat caccagtttg cgaacgagct gtcctgaacc agagcgagcg
     1981 cgtcaatgcc tcgcattccg cgcgaagatc gatgcgtcac tctcacccat tgaacttcat
     2041 gaacgagttc gcggccagcc gccgcacctc gctcggcagg ccacaggccg cgagaggcgc
     2101 cttggtctgc ctctcgaatc ggcctgacgg gtagttcacg ggtcaagacc cgcttcatac
     2161 gcagacggcg cgtgggtctc aggacttgcc gtatctcgac ttcgcggcac tgaggcatgc
     2221 gtctttcgcg gcgccggcga gtgcatcgca cttctcgatc gcgaccttgt attcggcctc
     2281 gcgtttttcc gcagtcgcat ccttgcgggc gtccgcctgc gtcgtcgccg cgtccttcct
     2341 cgtatcggcg gtgactcggt cgaccttggc gtctgccttg cccttcacca acgcggcctt
     2401 tgcttccttc acgcagacat ccttggtgtt acctgcctgg tcgtcgcact tttggatggc
     2461 gacatcgtaa gttgcctggg catgggcgac gcgggcactt tcgcgagcct tcggtgtgtg
     2521 cttgtaggcg gcttctgtgt cggccttggc gacgttgtgc ctgcccgtgg cctccgcggc
     2581 acagatgtcc ttggcattcc cggagagcga tgcgcacgcg ctcttggcag atttgtactg
     2641 cgcgtcggcg tccttcatcg cctgggtgta gttcgccttc gacatcggcg cagtagagac
     2701 catctgcgcc atgctggctc cggcgaaacc gacgctagcg ccagcaagca atgcaatcat
     2761 tagtttggac atacttcttc cttacaattc acgttcgtta tcgacaccct caaccccgcg
     2821 cgccgcagcc gtggccttgt tctcggccgt cgccgaacgc acaggtccgc tgaggcaatc
     2881 cccgatcttt aactcctgaa cacagagtta tcggagctcg ccggcagagg tcaggcgctc
     2941 ttgatcacat ccttcacgtc tttcttgacg tcgccaaact tggcttgagc ctcgccaaga
     3001 accttctgag tcttgccctt ctgctccagt ttttcattgc cgacaagctt gcctgcggct
     3061 tccttgaccg tccctttggc ttccttgatg cggcccttga cctggtctct gttcatgctc
     3121 atcgcttgct ccttatgacg tacgccggta agcgaccggc acgccgtact cctgtccctg
     3181 aagctgtggc tcgacgggag gaatgtccgc ctggacttcc ctcgtgccgt ggaccggcac
     3241 ccgaattccg gaccgcaacg taagcaatcg tggaacaccc acggttgtgg catcgagcgg
     3301 acgcccggcc cggagaacga ccggggcggc gcgtccattt gattgctcct gagcagccat
     3361 cagaccgctc gacatctact tcccgtcgcg atccgaatcc cctggcgtcg cgcgttctac
     3421 gaagtcgcta aggcgctgcc acgagtcacg ggtcgcgtgc ttggcgttct cccaggtcaa
     3481 tttggacgtg ccttttgctc cgtcccactc gcgcatcagt tcgggctccg cctgttcgaa
     3541 cgaacgacct tcgaaccgtc cgtagttatc gacgccgtag cgataggcgg ggccgtactc
     3601 gtcaaacgtc gccccgctgg tgacataggg cctgctcgaa tagttctggc gccaataggc
     3661 ttcctcggca gtggggtcga ccatcttggc gatccctttg ccagccaagg cacccgccac
     3721 cgcaccggcc gccgcgccga tcaccgtgcc cacagggccc gccacgctgg ccaccgctgc
     3781 gccagctgcg gcgcccccgg caactgcacc gagcccggtt ccaacaggat gcgttccaag
     3841 cggacccgag gccggatccg gttttaccgt cttgttcttg tcgagttcca tcgtgctgtt
     3901 tgtcatcgtc atctccaagt aattcatata ccaatcgatc aggtcttcgc ctaaagtaaa
     3961 actcgcgtgt ggatgcaaga cgtcggtccg cggagcgaac cacctaacgt ccagggtgga
     4021 gtcaccttta cccggggcgc atttgcgttc gctgaaggcg caatcagtgt gatggccatc
     4081 gatcctgccg ccacgattag tcgcgacaga tggagtgtcg gcgccgtcct tctgcttgtc
     4141 tgtgcggtgg cgcacacgac gggaacggct gcttttgcgc agacaatgcg cgtagaggtt
     4201 gccgacacgg atctccgcgt aaccttcacc tgcgaccgat gatcgaaagc ggtcgaccgc
     4261 gccgcaccac cgccacctca cccaccgggc aatgcagcgc gcagagttcg cgcaatctcc
     4321 tcccgagctc tcggcaaatg caaaagctgg aacatcgcaa acagatcgac ggacttcggt
     4381 tcttcgcgtt cctgccggtg ttcctggtgc acagcgatgt cacgactttc tggtggggca
     4441 gttatggcgt ctcgcttttc ttcgtgatca gcggttttct gatcacccga atcctgatca
     4501 cgtttgaaaa ccggccgcgc aggcagatgc tcaaaagctt ctatgcgcgt cgcgcgttgc
     4561 gaatctttcc cgcctactac ctggtgctgg gactggcggc agcgacggtc ggcatcgctt
     4621 acctgcctgc atttgccctg tacgtgatga actggggaat cttcttctac acgtggatcc
     4681 agcctgaact gttggacaaa gcgtgggatc cgacgcggat gtacggcatc cacttctggt
     4741 cgttgtgcgt cgaagagcag ttctatctcg tcttcccgct gctgttttcc ttcctgcgga
     4801 cggggcgtac gaggctgacg tggttgctcg ccctatgggc cgcgtctatc ggctttcgct
     4861 tcctctgcgc cgccttcctg cccacggcgg cgtatgggct gattccactg gtctgcggcg
     4921 aatatctggt cgcgggtgcg gtgggcgctg tggtattcgc ggatacatcg ggaaccgccg
     4981 aaaggatacg cgctgccgcc cggcaatgga tgtttcccgt tggcctcgcg ctggtggtgg
     5041 cactgttcgc gctgtatgga ccggagacac cgtcgatcga ccacgtcatg cacccgccgc
     5101 acatgcaaac gctgctagcg ctcggcttcc tcgcgatggt gctgggattg tggcaaacgg
     5161 acaaccgttg gttgctcggt tttttcaact acggaccctt tcgacaagcg gccacatgaa
     5221 gaggtggatg agatacaggc cgtagctgat cttgccgagg tatcgaaagg gaccgtagtt
     5281 gaaaaaaccg agcaaccaac ggttgt
//
LOCUS       DATIRT010000491        11979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_481199, whole genome shotgun sequence.
ACCESSION   DATIRT010000491 DATIRT010000000
VERSION     DATIRT010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11979
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481199"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3648)
                     /locus_tag="VLN25_11265"
     CDS             complement(<1..3648)
                     /locus_tag="VLN25_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HSQ11170.1"
                     /translation="MNAPVPHQGLRSTPAVLEPPVRLREIPYNYTSFSDREIVIRLLG
                     AEAWRVLDELRSERRTGRSARMLYEVLGDIWVVQRNPYLQDDLLDNPKRRALLIEALH
                     HRLGEVDKRREQAPATESDRERDAKVGQLLAAARKAVQSFEDAFRTTWDLRKEARREL
                     VRHTRDDNVRFDGFARVSHVTDATDWRVEYPFVVLQPDTEDEIPGLVRACISLGLTII
                     PRGGGTGYTGGAIPLTSLSAVINTEKLEALSEVEVLAQPGNGTPVPTVFSEAGVVTKR
                     VADAAERGGYVFAVDPTSADSSCVGGNIAMNAGGKKAVLWGTAVDNLAWWRMVDPEGN
                     WLEVQRVGHNLGKIHDATEAVFELTWKDGHQLAAKAQTLRRERLAIPGARFRKVGLGK
                     DVTDKFLGGVPGVQKEGCDGLITSARWIVHRMPKHIRTVCLEFFGRARDAVPSMVEIT
                     DYLERRPGGAVLAGLEHLDERYLRAVGYATKSRRGGLPKMVLIGDIVGDDDSAVAHAA
                     SEVVRMANGRTGEGFVAVSAEARKKFWLDRAKTAAISRHTNAFKLNEDVVIPLPRMGE
                     YTDAIERINIELSIENKLELLDALDSFLASPFAIGKTDDVEIDRLPRSEILGDRVEQA
                     RELIAYTRTRWQWLLTHFDASLADALPELTQLGLTHLTRAQSPDDGDRRPAVRVIDLL
                     QDHAIRVSWKSEVRAPLERIFAGAAFALVLAECEAIHARVLRGRVFVALHMHAGDGNV
                     HTNIPVNSDNYAMLQRANAAVARIMQIARDLDGVISGEHGIGITKLEFMTDAETADFR
                     RYKNTVDPEGRFNKGKLLPGADLSNAYTPSFGLMGHESLIMQQSDIKSISDAIKDCLR
                     CGKCKPVCSTHVPGANLLYSPRNKILATSLLVEAFLYEEQTRRGVSIKHWDEFGDVAD
                     HCTVCHKCLAPCPVDIDFGDVSMAMRDLLRRMGKKKFNPGTAASMFFLNVKDPQTIKV
                     ARTLMLEWGYKAQRIGVDLFRRLGRQQTRRPPATTGGRPQVTAQVIHFVNKKMPGDLP
                     KKTARALLDIEDARYVPIIRDPQRTTSESEAVFYFPGCGSERLFSQVGLATQAMLWHT
                     GVQTVLPPGYLCCGYPQRGAGQFDKAEKIITDNRVLFHRVANTLNYLDIKTVVVSCGT
                     CYDQLQDYKFEEIFPQCRIVDIHEYLLEKGVRIEGVGGVRYMYHDPCHSPMKQQDPLI
                     TVNALVATADGT"
     gene            3755..4147
                     /locus_tag="VLN25_11270"
     CDS             3755..4147
                     /locus_tag="VLN25_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HSQ11171.1"
                     /translation="MRLTSDISSQRPGVLLLRFTVAGLMLFHGIAKLRNGLEGIESLL
                     AGVGLPVWLSYGVLVGEVLAPLLVLAGLWVRPAALVMAFTMVVAVGLAHPTQLFTMGR
                     SGGYGLELQAFFLFCSVAIALLVDRRRM"
     gene            complement(4862..5881)
                     /locus_tag="VLN25_11275"
     CDS             complement(4862..5881)
                     /locus_tag="VLN25_11275"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HSQ11172.1"
                     /translation="MEDSAASTDLPYSGLTPDVVLNALDSAGLATTGSLLALNSYENR
                     VFQVGIDTDDGLPGFVVAKFYRPGRWTDAQILEEHDFVAELAAAEISAVPPLQLRDRT
                     LNTFEGFRFAVFKRRGGRAPLLDDPKVLEWIGRFLGRLHAVGRVHAFEARPVLDIDSF
                     GREPRAWLLAQGWLPPELRTVYEGVSQQALDEVRRAFDRAGDVARMRLHGDVHEGNVL
                     WYDGLTDHGPHFVDFDDARTGPAVQDLWMLLGADRTEQTQRLADLLAGYEDFSEFDRR
                     ELHLVEALRTLRLMHYAAWIARRWSDPAFPAAFPWFDTPRYWEDRILELREQTAAMQE
                     SPLVA"
     gene            5956..7482
                     /gene="ilvA"
                     /locus_tag="VLN25_11280"
     CDS             5956..7482
                     /gene="ilvA"
                     /locus_tag="VLN25_11280"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HSQ11173.1"
                     /translation="MPAQEYLRRILTARVYDVAIESPLEDAPKLSSRLGNRVLLKRED
                     LQPVKSFKLRGAYNKMARLDRVQLKRGVIAASAGNHAQGVALAAQKLGCRAVIVMPVT
                     TPQVKVEAVRARGADVVIFGDSYSDAYAHALEVQAKKKLTFVHPFDDPDVIAGQGTIA
                     MEILRQHHPVEHGPIHAVFVAIGGGGLAAGVGAYIKALAPEIRVIGVQTIDSDAMARS
                     IAAGRRVQLHDVGLFSDGTAVSQVGEETFRLCRKYVDEIVLVDTDAICAAIKDVFADT
                     RSILEPAGALAIAGMKFYAARDRLKGKTMVAIACGANMNFDRLRFVAERTEVGEAREA
                     LFAVTIPEERGSFKRFCGLVGSRSVTEFNYRISDASAAQVFVGISTANAGDNATLARS
                     FRQHGYATVDLTHDELAAEHVRYMVGGRSVLAAGELIYRFEFPERPGALMKFLTSMAP
                     GWNISMFHYRNQGADYSRVLVGLQVPASERREFARFLADLGFRHWDETGNPAYRLFLG
                     "
     gene            7537..8019
                     /locus_tag="VLN25_11285"
     CDS             7537..8019
                     /locus_tag="VLN25_11285"
                     /inference="COORDINATES: protein motif:HMM:NF015317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HSQ11174.1"
                     /translation="MLRPILNASRFLVIAAVLGSLAASMALFAYGAAETVVVIAETIA
                     KGEISSKGAKVLALSFIEIVDLFLLGTVLLMIGLGLYELFIDSNLKLPEWLQIRTFDD
                     LKYKLVGVVIVVLAVMFLGHVVAWDGSRELLGIGTAIALVIAALTYFLSGKGGKSDKG
                     "
     gene            8181..8501
                     /locus_tag="VLN25_11290"
     CDS             8181..8501
                     /locus_tag="VLN25_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11175.1"
                     /translation="MSMMTDDTLAFSVAEVTMQEFLAAVLVAAREPASPTTVRPQPAR
                     PLIEYPVARRPAPRPTQVRTTAPCRSGATGKNAEPVTPSRVAGARAAYWMLLAGALAV
                     FVLN"
     gene            complement(8559..8981)
                     /locus_tag="VLN25_11295"
     CDS             complement(8559..8981)
                     /locus_tag="VLN25_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HSQ11176.1"
                     /translation="MTSTDPSAQPTVHRSGWVRAFFLAAGTLALVLGLIGIFLPLLPT
                     TPFVLLAAACYARGSRRFYDWLLAQRTFGPIIHEWQRHRSIPYRTKITAVALMSLTLG
                     ISIVFFVEPLWLQGLLAAMGVGLAVWLYRMPSRDRPRR"
     gene            complement(8978..11011)
                     /locus_tag="VLN25_11300"
     CDS             complement(8978..11011)
                     /locus_tag="VLN25_11300"
                     /inference="COORDINATES: protein motif:HMM:NF021639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11177.1"
                     /translation="MGWRIFRRFARRPDVETEAPELDQLLAAAMRARNAIDRADWVRA
                     ALRWIEKDLADAADSEGIARAHTRMRFLLQVSERETDGGRAVRAALTGLLNDLDLEQL
                     MAVGAIPRRAGFMTELYERCLAAVLPQPDFRKEPALLTGMLLGAPRTVAWIDLLPAED
                     ARRVARLFVGPESPAKVDPQLGAAMLTLASEAQAVGLASEMRRRFADSSALDSPFGQL
                     VAAVEAFLAARDDTSRSELEHVVDESLALLGTLHAKLTETGVSVDLFYLSERTRAQLV
                     RVLRVARWMSGEDPLHVLREVMNAVRRDAQSRGVVALWRENMRLLSRRIAERNAETGT
                     HYIAGTRAQYRQMLAISAGGGALMAIAVYLKFAITAAHMPPFWEGLFASLNYAGVFVL
                     IALAHFTVATKQPAMTAPALAARMRELERPGQVEALVSESAALVRSQVASVFGNLMAV
                     MPAVLAVALLWWLVAGEGPLSDAKARDVLRSHSILGPSFLFAAYTGVLLWLSGLFAGW
                     ADNAFTVRQLHAAIATNARRVHHLGADGARERADWWRDNIAGLAGNIGLGVLLGLTPL
                     VFAFFGLPIEVRHVTLSTGQAAVATFSLGLDALTTAPFWMAVAGIVVIGVLNVGVSFG
                     LALRVAMRAVDISPTNRATVYRAIRGRLISHPGEFLWPPLDAPIGAVHEQSSR"
     gene            complement(11018..11974)
                     /gene="ttcA"
                     /locus_tag="VLN25_11305"
     CDS             complement(11018..11974)
                     /gene="ttcA"
                     /locus_tag="VLN25_11305"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HSQ11178.1"
                     /translation="MQDDSTLVAAPAGDPTDPRRAARLAHENHKLEKRLLRLAGQAIG
                     DYSMIEAGDKVMVCLSGGKDSYSLLELLLTLRSRAPIDFDIVAVNLDQKQPGFPAEVL
                     PDYLRRRGVPFRIETQDTYSIVTRIIPEGRTMCSLCSRLRRGVLYRVAGELGATKIAL
                     GHHRDDILGTFFLNLFYGGRLKGMSPKLVSDDGRHVVIRPLAYVKEDDLARYAQVQQF
                     PIIPCTLCGSQPNLKRQEVKRMLQEWERRFPGRVENTFNALARVVPSHLMDTQLFDFA
                     ALRPSGQHRPDGDIAFDEDPCTSESEPIVESTEGVIRFAEAE"
BASE COUNT         2036 a         3881 c         3976 g         2086 t
ORIGIN      
        1 cgtgccgtcg gccgtggcca ccaaagcatt gaccgttatg agtggatcct gctgcttcat
       61 cgggctgtgg caggggtcgt gatacatgta gcgcacgcca ccgacaccct cgatgcggac
      121 acccttttca agcaggtact cgtgaatgtc gacgatccgg cattgcggga agatttcctc
      181 gaatttgtag tcctgcaact ggtcatagca ggtaccgcag ctcaccacca cggtcttgat
      241 gtcgaggtag ttcagcgtgt tggcgacgcg gtggaagagc acccggttgt cggtgatgat
      301 tttttccgcc ttgtcgaact ggcctgcgcc ccgctgcgga tagccgcagc acaggtaccc
      361 tggcggcagc accgtctgca ccccggtatg ccacagcatc gcctgcgtgg cgaggccgac
      421 ctgcgagaag agccgctccg agccgcagcc ggggaagtag aagaccgctt cggactcgct
      481 cgttgtgcgc tgcggatccc ggatgatcgg cacgtagcgg gcatcctcga tatcgagcaa
      541 cgcgcgcgcg gtcttcttcg gcaggtcgcc cggcatcttc ttgttgacga agtggatcac
      601 ctgcgcggtg acctgcggcc ggccgcctgt cgtggccggc ggtcggcgtg tctgctgccg
      661 gcccagccgc ctgaagaggt cgacgccgat tcgctgcgcc ttgtagcccc actcgagcat
      721 cagcgtgcgc gcgaccttga tcgtctgcgg atccttgacg ttcaggaaga acatcgacgc
      781 cgcggtgccc ggattgaatt ttttcttgcc catgcgccgc agcaggtcgc gcattgccat
      841 gctgacgtcg ccgaagtcga tgtcgaccgg gcacggtgcc aggcatttgt ggcaaacggt
      901 gcaatggtcg gcaacgtcgc cgaactcgtc ccaatgcttg atcgagacgc cgcgccgcgt
      961 ctgctcctca tagagaaacg cctcgaccag cagcgaggtc gcgaggatct tgttgcgcgg
     1021 cgagtagagc aggttggcgc ccggcacgtg cgtcgagcac accggcttgc acttgccgca
     1081 gcgcaggcag tccttgatcg catcggagat cgacttgatg tcgctctgct gcatgatcag
     1141 cgactcgtga cccatcaggc cgaagctggg cgtgtaggca ttcgacaggt ccgcacccgg
     1201 cagcagcttg cctttgttga atcgtccctc gggatcgaca gtgttcttgt agcgccggaa
     1261 gtccgccgtc tcggcgtcgg tcatgaattc gagcttggtg atgccgatgc cgtgctcgcc
     1321 cgaaatcacg ccgtcgaggt cgcgggcaat ctgcatgatg cgggcgaccg ccgcattggc
     1381 ccgctgcagc atcgcgtagt tgtcggagtt gaccggaatg ttcgtgtgca cgttgccgtc
     1441 gccggcatgc atgtgcaaag cgacaaaaac gcgtccgcgc agcacgcggg catggattgc
     1501 ctcgcactcg gccagcacca gcgcgaaggc tgccccggca aagatccgct cgagtggcgc
     1561 ccgtacctcg gacttccacg acacgcgtat cgcatggtcc tgcagcaggt cgatgacccg
     1621 gaccgctggc cgccgatcac cgtcgtcggg cgattgagcg cgggtcaggt gcgtgagccc
     1681 gagctgcgtc aactccggca gcgcatcggc aagcgatgca tcgaagtgcg tcagcagcca
     1741 ctgccagcga gtgcgcgtgt aggcgatcag ctcgcgcgcc tgctcgacgc ggtcgccgag
     1801 aatctcggac cgcggcaggc ggtcgatctc gacatcatcg gtcttgccga tcgcaaacgg
     1861 cgatgccagg aacgaatcga gcgcgtcgag taactcgagc ttgttttcga tcgacagctc
     1921 gatgttgatg cgttcgatcg catcggtgta ctcgcccatg cgcggcaacg ggatgacgac
     1981 atcctcgttg agcttgaatg cgttggtgtg ccgggagatc gcggccgtct tggcgcgatc
     2041 gagccagaac ttcttgcggg cctcggcgct gacggcgacg aagccttcgc cggtccggcc
     2101 attggccatt cgcacgactt ccgaggcggc gtgcgcgacg gcggaatcgt cgtcaccgac
     2161 gatgtcgccg atcagcacca tttttggcag accgccgcgt cgcgacttgg tcgcataacc
     2221 gactgcacgc agatagcgct cgtcgaggtg ttccagcccg gcgagcaccg ctccaccagg
     2281 acgacgctcg aggtaatcgg tgatctcgac catggaaggc acggcatcgc gggcgcggcc
     2341 gaaaaattcc aggcaaacgg tgcggatgtg cttcggcatc ctatgcacga tccaccgtgc
     2401 cgacgtgatc agcccgtcgc agccttcctt ctgcacgccc ggcacgccac cgaggaattt
     2461 gtcggtgaca tccttgccaa gaccgacctt gcgaaaccgt gcaccgggaa tcgcgaggcg
     2521 ctcgcggcgc agtgtctgag cctttgcggc gagttgatgg ccatccttcc acgtcagctc
     2581 gaaaacggcc tcggtagcgt cgtgaatctt gccgaggttg tggccgacgc gctgcacttc
     2641 gagccagttg ccctcggggt cgaccatgcg ccaccacgcg aggttgtcga ctgccgtacc
     2701 ccagagcacg gccttcttgc cgccggcgtt catcgcgatg ttgccgccga cgcaagatga
     2761 gtcggccgag gtcggatcga cggcaaatac atacccacca cgctcggcgg catcggcgac
     2821 gcgcttggtg accacgccgg cctcggaaaa gaccgtgggt actggcgtgc cgtttcctgg
     2881 ctgcgccagc acctcgactt cggacagcgc ctccagcttc tcggtgttga taacggccga
     2941 cagcgacgtg agcggaattg cgccgccggt gtagccggtg ccgccgccgc gcgggatgat
     3001 ggtcaggccg agcgagatgc acgcgcgcac cagccctggg atctcgtcct cggtgtccgg
     3061 ctgtagtaca acgaacgggt attcgaccct ccagtcggtg gcgtcggtca cgtgcgacac
     3121 gcgcgcgaaa ccgtcaaagc ggacgttgtc gtcgcgcgtg tggcgcacga gttcgcggcg
     3181 cgcctccttg cgcaggtccc aggtggtacg aaacgcgtcc tcgaagctct gcacggcctt
     3241 gcgcgccgcc gcgagcagct gaccgacctt ggcgtcgcgc tcgcgatcgc tctcggtcgc
     3301 cggggcctgc tcgcgccgct tgtcgacctc gcccagccgg tggtgcagcg cctcgatcag
     3361 caacgcgcga cgcttgggat tgtcgaggag gtcgtcctgc aggtacgggt tccgttgtac
     3421 gacccagatg tcgcccagaa cttcgtagag catccgcgcc gaacggcccg tgcggcgttc
     3481 gctgcgcagt tcgtcgagca cgcgccacgc ttcggcgccg agcagccgga tcacgatctc
     3541 gcgatccgag aacgaggtgt agttgtacgg aatctcgcgc agtcgaaccg gtggttcgag
     3601 tacggcggga gtggagcgca atccctggtg cgggacggga gcgttcatga ggtgtggacc
     3661 ggccgttggc ccaggtggac gcttgggggg caattgtaac ttttgcgccg cagcgaggca
     3721 gaatccatcg tcccgttaca gcgaacccac ctcgatgcgc ctcacctccg atatctccag
     3781 ccaacgcccg ggcgttctgc tcttgcgatt cacggtcgcc ggactcatgc tgttccacgg
     3841 gattgcgaag ttgcgcaatg gcctggaggg gatcgaatct ctgctcgcgg gtgtcgggct
     3901 tccggtctgg ctttcctatg gagtgctggt cggtgaagtc cttgcaccct tgctcgtcct
     3961 tgccggcctt tgggttcggc ctgccgcgct ggtgatggcg ttcaccatgg tcgttgccgt
     4021 aggactggct cacccgaccc aactgttcac gatgggtaga agcggtggtt atggccttga
     4081 gctgcaggcg ttctttctct tctgctccgt tgctatcgca ttgcttgtcg atcggaggcg
     4141 gatgtgacct cgcgcatcga gatcgtccat cagcagacga gtcgaacgtc gatttaagtc
     4201 agcctcgcat gaggatggcc ggtgggcccg cgggcacgcc agttgccttg ttcatcttgc
     4261 ctttgcctgc cggcggggcg ctgaacagct cggccaacgt cgtacccaca atgccaacga
     4321 tcgcgttcag ggccccgtgc gttcggcagc gcacagacct cgccggtctg agtggttaag
     4381 gttcgaacat gggcagggtc ttgcttgccg tctttggaaa tggaggaata cgatgaagcg
     4441 tttcactttt atctttgcag cgtgcgccag tgcgcttgtc ttcaccgccg gatgtaacag
     4501 gaccgacact cccccagctc cgaccgcaac accgccaacg aacaccgcac cgcaggctgc
     4561 cacggcaccg accacaccgc ccgtacagga ggctccgtcg acgctgaccg cgccgatgcc
     4621 gactcccgat gtgcccaagg gcgacgcttc gtccggcccg aaacctggcc aggcgaacga
     4681 tcattcaagc ccggcgttca aggggggtgg caaggaagtt cccaagtaga tcgatccctt
     4741 tgtcgctccg ggagcgtcga ccttggcccg tcggcccagc ttgaaagcgg gcacggcggc
     4801 accgttcctc agcattcgcc ggtcgttgtg agctccgctt cgcgacagac cggccagtag
     4861 ttcaggcgac gagcggcgac tcctgcatgg cggctgtctg ttcgcgtagc tcgaggatgc
     4921 gatcctccca atagcgcggc gtgtcgaacc acggaaacgc ggcaggaaag gccggatcgc
     4981 tccagcgccg cgcgatccat gcggcgtaat gcatcagacg cagggtccgc aaggcttcaa
     5041 ccaggtgcag ttcgcgccgg tcgaactcgc tgaagtcctc gtagccggcg agcagatcgg
     5101 cgagacgctg cgtctgctcg gtgcggtccg ccccgagcag catccacagg tcctgcacgg
     5161 ccggtccggt acgcgcatcg tcgaagtcga cgaagtgcgg gccgtggtcg gtcaggccgt
     5221 cgtaccacag gacattgccc tcgtgcacgt cgccgtgcag gcgcatgcgc gcaacatcgc
     5281 cggcgcgatc gaaggcgcgc cgcacttcgt cgagtgcctg ctgcgagacg ccttcataga
     5341 ctgtgcgcag ttcgggcggc agccacccct gcgcgagcag ccaggcgcgc ggttctcggc
     5401 cgaagctgtc gatgtccagc acgggccgcg cctcgaacgc gtgcacgcgg ccgacggcgt
     5461 gcagtcgacc gaggaagcgg ccaatccact cgagaacctt ggggtcgtcg agcagcggcg
     5521 cccgtccgcc gcggcgcttg aagacggcaa agcgaaagcc ctcgaaggta ttgagcgtgc
     5581 ggtcgcgcag ctgcagcggt ggcaccgccg agatttcggc ggcggccagc tcggcaacga
     5641 aatcgtgctc ctcgaggatc tgcgcatccg tccaacgccc cggccggtag aacttggcga
     5701 cgacgaagcc gggaagtccg tcgtcggtgt cgatgccgac ctggaacacc cggttttcgt
     5761 agctgttgag tgccaacagc gaccccgtcg tcgccagccc tgcactgtcg agcgcattca
     5821 agaccacgtc cggcgtcaga ccggagtagg gcagatcggt gcttgcagct gagtcttcca
     5881 tggacaccat ggtaggccag cggaaccgtc ggttgcgcgt ggtaccttcg cacagatcga
     5941 ggaggccgag gcacgatgcc tgcacaggaa tacctgcgcc gcatattgac ggcgcgcgtc
     6001 tacgacgttg cgatcgaatc cccgctggag gacgcaccga agctgtccag tcgactcggc
     6061 aaccgcgtgc tgctcaagcg cgaagacctg cagccggtca agagcttcaa gctgcgcggc
     6121 gcttacaaca agatggctcg gctcgaccgg gtgcagctga agcgcggcgt catcgcggca
     6181 tcggccggaa accacgcaca gggcgtagcg ctggcagcgc agaagctcgg ctgccgggcc
     6241 gtcatcgtca tgccggtgac gacgccgcag gtcaaggtcg aggcggtgcg tgcgcgcggt
     6301 gccgacgtgg tcattttcgg cgattcgtat tccgacgcct acgcgcacgc actcgaagtg
     6361 caggcgaaga agaagctcac cttcgtccat ccgttcgatg accctgacgt catcgccggc
     6421 cagggcacga tcgcgatgga gatcctgcgc cagcaccacc cggtggagca cgggccgatc
     6481 cacgcggtgt ttgtcgcgat cggcggagga gggcttgccg ccggcgtcgg ggcctacatc
     6541 aaggcgctgg cacccgagat ccgcgtgatc ggggttcaga ccattgactc ggatgcgatg
     6601 gcgcgttcta tcgcggcggg tcggcgcgtt cagttgcacg acgtcggcct gttttcggat
     6661 ggcaccgcgg tcagccaggt cggcgaagaa acgttccgcc tctgtcgcaa atacgtcgac
     6721 gagattgtcc tggtggacac cgatgcgatc tgcgcggcca tcaaggacgt ctttgccgat
     6781 acgcgctcga tactcgagcc cgcgggcgca ctggcaattg ccggcatgaa gttctacgcg
     6841 gcgcgcgacc ggctcaaggg caaaacgatg gtggcgatcg cctgcggggc gaacatgaac
     6901 ttcgaccgcc tgcgcttcgt cgccgaacgc actgaagtgg gcgaagcgcg cgaggcgctg
     6961 ttcgcggtaa cgatccccga ggagcgcgga agcttcaagc gcttctgcgg actcgtcggc
     7021 agtcgcagcg tgaccgaatt caactatcgc atcagcgacg cgtcggcggc ccaggttttc
     7081 gtcggcatca gcacggccaa cgcaggcgac aacgccacgc tggcgcgcag cttccgtcag
     7141 cacggctatg ccacggtcga cctgacccac gacgaactgg cggcggagca cgtgcgctac
     7201 atggtcggcg gccgcagtgt cctggccgcc ggcgagctga tctaccggtt cgaatttccg
     7261 gagcggcccg gcgcactgat gaagttcctg accagtatgg cgccgggctg gaacatctcg
     7321 atgttccact atcgcaacca gggggccgat tactcgcgcg tgctggtcgg gctgcaggtg
     7381 ccggcatccg agcggcgtga atttgcgcgc ttcctcgctg atctcggttt tcgtcactgg
     7441 gacgagaccg gcaatccggc gtatcgcctt tttctgggct gagccggcgg ccggcggctc
     7501 cgggccaatt cctgttgcag acctccgggt gacaccatgc tgcgtccgat actgaatgcc
     7561 agtcgcttcc tcgtcatcgc tgcggtgctg gggtcgcttg ccgcttcgat ggcgctgttt
     7621 gcttatggtg cggcggaaac cgtcgtcgtc atcgcagaga cgattgccaa gggtgaaatc
     7681 tcgagcaagg gcgcgaaggt cctggccctg tcgttcatcg aaatcgtgga cttgtttctg
     7741 ctgggtacgg tgctgctgat gatcggcctg gggctgtatg agctcttcat cgactccaac
     7801 ctcaagctgc ccgagtggct gcagatacgc acgttcgacg atctcaagta caagctcgtc
     7861 ggcgtcgtga tcgtcgtgct ggccgtgatg tttctcggcc acgtcgtcgc gtgggacggt
     7921 tcgcgcgagt tgctcggcat cgggaccgcg attgcgctgg tgatcgcggc gctgacctac
     7981 ttcctttcag gcaagggcgg caagagcgac aaaggctgat cgaaatcgga tcggctcgcc
     8041 cgtcagggcg gtcgggcgga gcgttcctgt cgaattttca gcacaggcgc cgctgaaccc
     8101 gccgcagccg acttcgccgg cgcccgccat catgccttcc gtccagcgat cgctggatcc
     8161 aaaaacgggc gggggccgac atgagcatga tgaccgacga taccttggca ttcagcgtcg
     8221 ccgaggtgac gatgcaggag ttcctggcgg cagtgctggt ggccgcccgc gagccggcct
     8281 cgccgacaac ggtccggccc cagcctgcgc ggccgctgat cgagtatccg gtcgctcgcc
     8341 gccccgcccc tcgcccgact caggtcagga cgaccgcccc ttgccgcagc ggtgcgacgg
     8401 ggaagaacgc agagcccgtg acgccgtcgc gagtggccgg agcgcgtgcc gcgtactgga
     8461 tgctgctggc gggcgcgctc gcggtcttcg tgctgaacta gggtctcctc acgctgacgg
     8521 ggagtgcgaa ggccaatggc cggaggcagt gcgatgcgct agcgccgagg tcggtcgcgc
     8581 gacggcatgc ggtagagcca taccgccagc ccgacgccca ttgccgccag cagtccctgc
     8641 agccacagcg gctcgacgaa gaacacgatc gatatgccca gggtcagcga catgagcgca
     8701 acggcagtga tcttggtcct gtacggaata ctgcggtggc gctgccactc gtggatgatc
     8761 ggcccgaacg tgcgctgcgc aagcagccag tcgtaaaagc ggcgtgaacc gcgcgcgtag
     8821 caggcggcgg ccagcaggac gaatggcgtg gtcggcagca acggaagaaa aattccgatg
     8881 aggccgagca cgagagccaa tgtgccggcg gccaggaaga aagctcgaac ccaccccgaa
     8941 cggtgcacgg tcggctgggc gctcgggtca gtcgacgtca acgcgagctc tgttcatgca
     9001 ccgcgccgat cggcgcgtcg aggggcggcc acaggaactc accggggtgc gagatgagcc
     9061 gaccgcggat cgcgcgatac accgtggccc ggttggtggg cgaaatatcg accgcgcgca
     9121 tcgcgacgcg cagggccagt ccgaaactga caccgacatt gagcacgccg atgacgacaa
     9181 tgccggcgac cgccatccag aagggcgccg tcgtcaatgc atcgagcccg agcgagaatg
     9241 tcgcaaccgc cgcttggccg gtggacagcg tcacgtggcg cacttcgatc gggagtccga
     9301 agaacgcgaa caccaggggt gtcagtccga gcaggacgcc gagaccgatg ttgccggcga
     9361 gtccggcgat gttgtcgcgc caccagtcgg cgcgctcgcg cgctccatcc gctccgaggt
     9421 gatgaacgcg tcgggcgttg gtcgcaatcg cggcatgcag ctggcgcacg gtgaaggcgt
     9481 tgtcggccca cccggcaaag agccccgaga gccacagcag cacaccggtg taggcggcga
     9541 acaggaacga cggccccagt atcgagtgcg atcgcagcac gtcgcgcgcc ttggcgtcgg
     9601 agagcggccc ctcgccggcg accagccacc agagcagcgc gacggcgagt acggctggca
     9661 tcaccgccat caggttgccg aacacggagg cgacctggga ccggaccagc gccgcgctct
     9721 cgctcaccag tgcctccacc tggcccgggc gttcgagttc acgcatgcgc gcggccaatg
     9781 ccggggccgt catcgcgggc tgtttggtgg cgaccgtgaa gtgcgccagc gcgatcagca
     9841 cgaacacgcc cgcgtagttc agcgaagcga agagaccctc ccagaacggc ggcatgtgcg
     9901 cggccgtgat cgcgaacttg aggtacaccg caatcgccat cagcgcgccg ccgccggcac
     9961 tgatcgccag catttggcgg tactgcgcac gagtgcctgc gatgtagtgt gttccggtct
    10021 ccgcattgcg ctcggcgatc cggcgcgaca gcagccgcat gttctcgcgc cacagggcaa
    10081 cgacgccgcg cgactgcgcg tcccggcgca cggcgttcat cacctcccgc aggacgtgca
    10141 gcggatcctc gccggacatc cagcgtgcca ctctcagcac acgaacgagt tgggcccgcg
    10201 tccgttcgct gagatagaac aggtcgaccg agacgccggt ctccgtcagc ttggcatgca
    10261 gtgtccccag cagcgccagg ctttcgtcga cgacgtgttc aagttcgctg cgagatgtgt
    10321 catcacgggc ggccaggaag gcctccacgg cggcgaccag ctggccgaag gggctgtcga
    10381 gggcactgct gtcggcaaag cgccggcgca tttctgatgc aaggccgaca gcctgggctt
    10441 cggacgccag ggtcagcatc gccgcgccga gttgcggatc cactttcgcg ggtgattcgg
    10501 gcccgacgaa gagccgcgcg acgcggcgcg cgtcctcggc cggcagcagg tcgatccatg
    10561 cgacggttcg gggtgcgccg agcagcatgc cggtcagcag cgccggctcc ttgcgaaaat
    10621 ccggctgcgg cagcactgcc gcgaggcagc gctcgtagag ctcggtcatg aaccctgcgc
    10681 gccggggtat cgcgccgacc gccatcagct gttcgagatc taggtcattc agcagcccgg
    10741 tgagtgcggc gcgcaccgca cgtccgccgt ccgtttcgcg ttcggacacc tgcagcagga
    10801 atcgcatgcg tgtatgcgcg cgtgcaatgc cctcgctatc ggcggcgtcg gcgaggtcct
    10861 tctcgatcca gcgcaacgcc gcgcggaccc aatcggcgcg gtcgatcgcg ttccgcgcac
    10921 gcattgccgc ggccagcagc tggtcgagtt caggggcctc ggtctccaca tccgggcgcc
    10981 gcgcgaaccg cctgaaaatg cgccagccca tcacccttca ctcagcttcg gcgaagcgga
    11041 tcacgccctc agtcgactcc acgatcggct cggactccga cgtgcacggg tcctcgtcga
    11101 acgcgatgtc gccgtccggc cggtgctgac cggatggacg cagcgcggcg aagtcgaaga
    11161 gctgcgtatc catcagatgc gagggaacca cgcgggccag tgcgttgaac gtgttctcga
    11221 cgcggcccgg gaagcgccgc tcccactcct gcagcatgcg cttgacttcc tggcgcttga
    11281 ggttgggctg cgagccgcac agggtgcagg ggatgatcgg aaactgctgc acctgcgcat
    11341 agcgcgcgag atcgtcttcc ttcacgtagg cgagcggccg tatcaccaca tggcgaccgt
    11401 cgtccgaaac gagcttcggc gacatgccct tcaggcggcc gccatagaac agattcagga
    11461 agaaggtccc gaggatgtca tcgcgatggt ggccgagcgc gatcttggtg gcgcccagtt
    11521 cgcccgccac gcggtaaagg actccgcgac gcaggcgcga acacagcgag cacatcgtcc
    11581 tgccctcggg aatgatgcgc gtgacgatcg agtacgtgtc ctgcgtctcg atgcggaagg
    11641 gcacgccgcg ccggcgcaga tagtcgggca atacctcggc cggaaagccc ggctgcttct
    11701 ggtcgaggtt gacggccacg atatcgaagt cgatcggcgc gcggctgcgc agcgtcagca
    11761 gcagttcgag caggctgtag ctgtccttgc cacctgacag gcacaccatc accttgtcgc
    11821 cggcttcgat catgctgtag tcgccgatcg cctgcccggc gaggcgcagc aggcgctttt
    11881 cgagcttgtg attctcatgc gcgagcctgg ccgcgcggcg cggatcggtc gggtcgcccg
    11941 cgggcgcggc gacgagcgtg ctgtcgtcct gcagcgcgc
//
LOCUS       DATIRT010000492         7073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1857509, whole genome shotgun sequence.
ACCESSION   DATIRT010000492 DATIRT010000000
VERSION     DATIRT010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7073
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1857509"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(266..2122)
                     /locus_tag="VLN25_11310"
     CDS             complement(266..2122)
                     /locus_tag="VLN25_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HSQ11179.1"
                     /translation="MAVEGSAGDLLKVVGLLSAAVIAVPLFRRAGLGSVLGYLAGGLA
                     IGPFGLGWFTHPEAILHVAELGVVMFLFVIGLEMRPSHLWSLRGQIFGLGTLQVAVCS
                     ALLTGVAVLLGLPPVPAFIGATGFVLTSTAVVMQVLAERGDIALPRGQKIVSILLFED
                     LLIVPLLALVAVLSPGVSDAGGSQWLKVGMGVGALAVVIVVGIWLLNPLFRLLAAAKA
                     REVLTAAALLVVLGSALLMQLGGLSMAMGAFLAGVLLSQSSFRHQIEADIEPFRGVLM
                     GLFFLGVGMSLDLAVVARNWQLIAVAVLAFMAVKALAIYAVARLTKASHVEARERAVL
                     MAQGGEFAFVLYAAAVAAGVIDGEVNANFTAIVVLSMALTPLAVLIADSVLPKAKPST
                     EGIENADGLSGSVLMIGFGRFGQVVSQSLLARDVDVSIIDNDVEMIRSADDFGFKVYY
                     GDGTRLDVLRASGAATARAIAVCIDDKDAANRIVELAKHEFPQARLLVRSFDRQHSLE
                     LVQAGVDMQIRETFDAAMRFGEAALRELGVPTDEAAEIAAEVRKRDAARFELEIAGGD
                     VRAGRPLLRGNKWKPTPLIEPRHEGRALCEETEKAAQEADAAAVAERGAAAL"
     regulatory      2375..2483
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            2511..3947
                     /gene="ahcY"
                     /locus_tag="VLN25_11315"
     CDS             2511..3947
                     /gene="ahcY"
                     /locus_tag="VLN25_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HSQ11180.1"
                     /translation="MSAVLKPNPAGVDYVVADIKLADWGRKEIRIAETEMPGLIAIRD
                     EFASQQPLRGARITGSLHMTIQTAVLIETLHALGAEVRWASCNIYSTQDHAAAAIAAT
                     GTAVFAVKGESLEEYWDYTHRIFEWPDGGYSNMILDDGGDATLLLHLGSKAEKDASVI
                     SHPTSEEEESLFKSIKAKLATSPAWYSMRLAKIKGVTEETTTGVKRLYQMAKEGRLAF
                     PAINVNDSVTKSKFDNLYGCRESLVDGIKRATDVMIAGKVALVAGYGDVGKGSAQALR
                     ALSAQVWVTEVDPICALQAAMEGYRVVTMDYAADKADIFVTATGNFHVITHDHMKKMK
                     DQAIVCNIGHFDNEIDVASLKQYQWEEIKPQVDHIIFPAVDGKPGKRIILLAQGRLVN
                     LGCGTGHPSYVMSSSFANQVIAQIELFTNTEKYPVGVYVLPKHLDEKVARLQLRKLNA
                     QLTELTDVQARYIGVQPEGPYKPDHYRY"
     gene            4078..4422
                     /locus_tag="VLN25_11320"
     CDS             4078..4422
                     /locus_tag="VLN25_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018060201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HSQ11181.1"
                     /translation="MRLLLVWVLNAIALLGVAYIYPGVQVEDWKSAAIAALVLGLVNT
                     LVKPVLVLLTLPVTILTLGLFLVVLNALLFWGVAEVLPGFNVNGFWAAVLGAILYSLI
                     GWALSKLLPDGK"
     gene            4422..5261
                     /gene="metF"
                     /locus_tag="VLN25_11325"
     CDS             4422..5261
                     /gene="metF"
                     /locus_tag="VLN25_11325"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HSQ11182.1"
                     /translation="MVERTYSFEFFPPKTPDGEDKLRAVRTRLAALDPAFFSVTFGAG
                     GSTKEGTWNTVMSIHAEGLSVAPHISCIGTTHASVRELLHSYRNAGIRRLVALRGDMP
                     SGAAGGAGDFGHANELVEFIRNETGDWFHIEVAAYPEYHPQSKSPADDLANFVRKVKA
                     GASSAITQYFYNSDAYFRFVDAADSMGCSVPVVPGIMPITNYTQLARFSDACGAEIPR
                     WIRRRLESFGDDRESIRAFGLDVVTDLCDQLLVAGAPGLHFYTMNQAEPTLALWQRLD
                     LQR"
     gene            complement(5290..5646)
                     /locus_tag="VLN25_11330"
     CDS             complement(5290..5646)
                     /locus_tag="VLN25_11330"
                     /inference="COORDINATES: protein motif:HMM:NF022592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HSQ11183.1"
                     /translation="MDWGPTLVRTIDLGYEQMLLLESRPQTRVKVIYGGVWLTEEGLA
                     QDVFAASGEEVALKSRGLAVVEGLGYARVQVMEAPAGWRAVRAVVRRSLDAFEGWFHR
                     LPLAQRATPARSQSAA"
     gene            5773..6726
                     /locus_tag="VLN25_11335"
     CDS             5773..6726
                     /locus_tag="VLN25_11335"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HSQ11184.1"
                     /translation="MPPTPARPRRRRPIALAALRGFDAAARHLSFTLAATELNLTQSS
                     ISRQIASLEREVGRTLFERRTRALELTVAGERLYAVVRQALLAVDRTVDEIRGDGGTP
                     RVTITTYASFASLWLVPRLPDFQRLHPGFDIRIDASDRMVDLESDDVDLAIRWLRPGA
                     PVPTDATLLQTEESSPALSPRLLATRKSPLRDPGELGTLPILAMDESVPSAPYSSWAR
                     WCEFAGIAPFDGAARLYFTYVDQSVQAAVRGQGVALVRTPFLDDLVASGDLVMPFPHL
                     RMPTGYRYFMIVNRKRSAVPHVAAFRDWIVTEFELGPHRLT"
     gene            complement(6803..>7073)
                     /locus_tag="VLN25_11340"
     CDS             complement(6803..>7073)
                     /locus_tag="VLN25_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015856055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HSQ11185.1"
                     /translation="SMRAAEFNIPVPAHGRPVQPDALLIPCVGFDAQRYRLGYGGGYY
                     DRTLAQTVPWPLCVGIAFECGRLESIDRQTHDMQLDVVITDAGTF"
BASE COUNT         1305 a         2403 c         2224 g         1141 t
ORIGIN      
        1 tttacgtggg aacgaactga ccgtgcggcc gcgctagcgg acgcggtcgg ggtgaaacgc
       61 cgcgccgcct gaagggtagc ggatcaatct tccgtcgagg cttacttcgg cgccagccga
      121 agttaagcgg ccgtcaagcg acgggcgcgg ccggagacaa aagtcgacct cgcggcggcg
      181 ctggccgacg cggttggcgt gaagcgccgc gctgcctgag ctaagccgac ccgcgacagc
      241 aggaagccga catggcctgc gagcttcaga gtgcggcagc gccgcgctcg gccacggccg
      301 ccgcatccgc ttcctgcgcc gccttttccg tttcctcgca cagcgcccga ccctcgtgcc
      361 gcggttcgat cagcggcgtc ggcttccact tgttaccgcg cagcagtggt cgtcccgcgc
      421 gcacatcgcc gcctgcaatc tcgagttcga aacgcgccgc gtcgcgcttg cgcacctcgg
      481 ccgcgatctc cgcggcctcg tcggtcggca cgccgagttc gcgcagcgcc gcctcgccga
      541 agcgcattgc cgcatcgaaa gtctcgcgta tctgcatgtc gaccccggcc tggaccagtt
      601 ccagcgaatg ctgccgatcg aacgagcgca ccagcaggcg cgcctgcgga aactcatgtt
      661 tcgccagctc gacaatgcgg ttggcggcgt ccttgtcgtc tatgcacacc gcaatagcgc
      721 gcgccgttgc agcgcccgaa gctcgcagca cgtcgaggcg cgtcccgtcc ccgtagtaga
      781 ccttgaatcc gaagtcgtcg gcactgcgga tcatctcgac gtcgttgtcg atgatcgata
      841 catcgacgtc gcgcgccagc agcgactggc tgaccacctg gccaaagcgg ccgaagccga
      901 tcatcagcac gctgccgctc aggccgtcgg cgttctcaat gccctcggtc gacggcttgg
      961 ctttcggcag caccgaatcc gcgataagca ccgccagcgg cgtcagagcc atcgacagca
     1021 cgacgatcgc cgtgaagttg gcgttgacct cgccgtcgat aacgccggcg gcgacggcgg
     1081 ccgcatacag cacgaaggcg aactcgccac cctgggccat cagcaccgca cgctcgcgcg
     1141 cctcgacgtg acttgccttc gtcagccgcg ccacggcgta gatcgccagt gccttgacgg
     1201 ccataaaggc cagcaccgca accgcgatca gctgccagtt ccgggcgacc accgccaggt
     1261 ccaacgacat gccgacgccg agaaagaaca gtcccatcag aacgccgcgg aacggctcga
     1321 tgtcggcctc gatctggtgg cggaacgacg actgggacag cagtacgccg gccagaaacg
     1381 cacccatcgc catcgacagg ccgcccagtt gcatcagcaa cgccgagccc agcaccacga
     1441 gcagcgccgc ggccgtcaag acttcgcgcg ccttggctgc ggccagcagg cggaacagcg
     1501 gattcagcag ccagatgccg acgacgatga cgacggccag cgcaccgact cccatgccga
     1561 ccttcagcca ctgggaaccg ccggcatcgg acacgccggg ggacagcacc gccaccagcg
     1621 ccagcagcgg cacgatcagc aggtcctcga acaacaggat cgacacgatc ttctggccgc
     1681 gtggcagcgc gatgtcgccg cgctcggcca gaacctgcat gacgaccgct gtcgaggtca
     1741 gcacgaagcc ggtggcaccg atgaaggccg gcaccggcgg caatcccagc agcaccgcca
     1801 cacccgtcag cagtgccgag cagacggcaa cctgcagcgt gcccagcccg aagatctggc
     1861 cacgcagact ccacaggtgt gacggccgca tttccagccc gatcacgaac aggaacatca
     1921 ccacgccgag ctcggcgaca tgcaggatcg cctcggggtg cgtgaaccag cccagcccga
     1981 agggtccgat cgcgaggccg ccagccagat agccgagcac ggaacccagt cccgctcggc
     2041 ggaagagcgg caccgcgatg acagcggcac tcagcaggcc caccaccttc aggagatcgc
     2101 cagcactgcc ttccaccgcc attgccgctc cccgaaatca aatcctgcgc cagccgcggc
     2161 gactgaatag cacgaactca cgagtgcagc gaggtgtgcg aaaggtcccg gagcgaccgg
     2221 caggaaggca ccgcgtcttt cttcgtacgc gaaggaacgc cgcgcggagt cgatccgcac
     2281 cgttgtttcg tcgagggttt cgtcgaggcg gcccaagctt tggacgatgc gcgcctcata
     2341 gcgcccgccg atcgacgcgg gtacaatccg cgcctgcttc gaggagcgtt gcgacaggac
     2401 ttgtcaccag acaagccgcc tgccaggctc gaggcgccgg tccccacggg gagtgccggc
     2461 cgtgccaacg gcgctcgccc acttctttga cagctaaagg agggcgcgag atgagcgccg
     2521 tactcaaacc gaaccccgcc ggtgtcgact atgtcgtcgc cgatatcaag cttgcggact
     2581 ggggccgcaa ggaaatccgt atcgccgaaa ccgaaatgcc gggcctgatc gcgatccgtg
     2641 acgagttcgc cagccagcaa ccgctgcgcg gtgcacgcat caccggatcg ttgcacatga
     2701 cgattcagac ggccgtgctg atcgagacgc tgcacgcgct cggcgcggaa gtgcgttggg
     2761 ccagctgcaa catctactcg acgcaggatc acgccgccgc cgccatcgcg gcgaccggca
     2821 ccgcggtgtt tgcggtcaag ggcgagtccc tcgaagagta ttgggactac acgcatcgaa
     2881 tcttcgagtg gcccgatggc ggctactcca acatgatcct cgacgacggc ggcgacgcga
     2941 ccttgctgct gcacctgggc agcaaggcgg agaaggacgc gtccgtcatc tcgcacccga
     3001 ccagcgaaga agaagagtcg ctgttcaaat cgattaaggc caagctcgcc acgagcccgg
     3061 cctggtattc gatgaggctc gcgaagatca agggcgtgac cgaggagacc accaccgggg
     3121 tcaagcggct gtatcagatg gccaaggaag gccgcctggc gttcccggcg atcaacgtca
     3181 atgactccgt gaccaaaagc aagttcgaca acctctatgg ctgccgcgag tcgctggtcg
     3241 acggcatcaa gcgcgccacc gacgtaatga tcgccggcaa ggtcgcgctg gtcgccggct
     3301 acggcgatgt cggcaagggc tcggcgcagg cgcttcgcgc gctgtcggcg caggtctggg
     3361 tcacggaagt ggacccgatc tgtgcgctgc aggcggcgat ggaaggctat cgcgtcgtga
     3421 cgatggacta cgccgctgac aaggccgaca ttttcgtcac cgcgaccggc aacttccatg
     3481 tgatcacgca cgatcacatg aagaagatga aggaccaggc gatcgtctgc aacattggtc
     3541 acttcgacaa cgaaatcgat gtcgcgtcgc tgaagcagta ccaatgggaa gagatcaagc
     3601 cgcaagtcga tcacatcatc ttccccgcag ttgatggaaa accgggcaag cgcatcatcc
     3661 tgctcgcaca ggggcgcctc gtgaatctcg gctgcggcac cggtcatccg agctacgtga
     3721 tgtcgagttc gttcgccaac caggtgatcg cccagatcga actcttcacc aataccgaaa
     3781 agtatccggt cggcgtatac gtgctaccga agcatctcga cgagaaagtc gcgcgcctgc
     3841 agctcaggaa gctgaatgcg cagttgaccg aactcacgga cgttcaggcg cgctacatcg
     3901 gtgtgcagcc cgaagggccg tacaagccgg accactacag gtattgattg tgcgtcggaa
     3961 agtttcgcac cgggcgcgct gcgcccggtg cacgatactt tcctccgact agtttgcgcc
     4021 gcgcccttcg ggcggccggg cgagcgcgac gaattcaact gcagggggag gacaagcatg
     4081 cgtctgctgc tggtttgggt gctcaacgcg atcgcgctgc tgggcgtcgc gtatatctat
     4141 ccgggcgtgc aggtcgagga ctggaagtcc gcggcgattg ctgcgctggt gctgggtctc
     4201 gtgaacacac tggtcaaacc ggtcctggtg ctgctgactc tgccggtcac gatcctgacg
     4261 ctcgggctgt tcctcgtcgt gctcaatgca ctgctgttct ggggtgtggc cgaagtgttg
     4321 cccggattca acgtgaacgg cttctgggcg gccgtgctcg gcgcgatcct gtacagcctg
     4381 atcggctggg cgctttcaaa gctgctgccg gacggcaagt gatcgtggag cgcacttaca
     4441 gcttcgagtt ctttccgccc aagacgccgg acggcgagga caagcttcgc gcggtgcgaa
     4501 cgcgcctggc cgcgctggat ccggctttct tttcggtgac cttcggtgcc ggcggctcga
     4561 ccaaggaagg cacctggaac accgtgatgt ccatccacgc agaaggcctg tcggtggccc
     4621 ctcacatctc ctgcatcggc acgacgcatg cctcggtgcg ggaacttctg cacagctatc
     4681 gaaacgccgg aatccggcgc ctcgtggcac tccggggtga catgccctcg ggcgctgccg
     4741 ggggtgccgg tgacttcgga catgcgaacg agcttgtcga gttcatccgc aacgaaacag
     4801 gcgactggtt ccatatcgag gtggctgcct atcccgagta ccacccgcag tcgaagtctc
     4861 cggcggatga cctcgccaac ttcgtccgca aggtcaaggc cggcgcaagc tcggcgatca
     4921 cccaatactt ctacaactcg gatgcgtatt tccggttcgt cgacgcggcc gactcgatgg
     4981 gctgttcggt gccggtggtc ccgggcatca tgccgatcac caactacacg cagctcgcac
     5041 gcttttccga tgcgtgcggc gcagagatcc cgcgctggat acgtcgtcga ctggagagct
     5101 tcggagacga ccgcgaatcg atccgggcgt tcggactcga cgtggtcacg gatctgtgcg
     5161 atcagttgct ggtggcgggc gcgcccggcc tccacttcta cacgatgaac caggccgagc
     5221 ccaccctggc gctgtggcag cggctcgacc tgcagcgctg acggcgggcc acgccccgcc
     5281 gaggtgcttt caggctgccg actgcgaccg ggccggcgtc gcacgttggg cgagcgggag
     5341 tcggtggaac cagccctcga aggcatcgag tgagcggcgc acgacagcgc gaacggcacg
     5401 ccacccggcc ggcgcttcca tgacctggac cctcgcatag cccaagccct cgacgacggc
     5461 cagcccgcgc gacttcagcg ccacttcttc accactcgcg gcaaacacgt cctgcgccag
     5521 gccttcctcg gtcagccaga caccgccgta gatcaccttc acgcgcgtct gcgggcgcga
     5581 ctccagcagc agcatctgct cgtagcccag gtcgatcgtc cgtaccagtg ttggacccca
     5641 gtccatgatg ttctcctatg cggaacttgc atccgttgag caagtacgga cacatttgac
     5701 gcctctggaa gacacgggac aaacgaaaag gtcgcaggct atgcatgcac caggcgcatg
     5761 tatagtaccg ccatgccgcc gactcccgca cgcccgcgcc gacgacgccc gattgcgctc
     5821 gccgcgctgc gcggattcga tgcagccgca cgccacctgt ctttcacgct ggctgcgacc
     5881 gaactgaacc tgacgcagtc gtcgatcagc cggcagatcg cctcgctcga acgcgaggtc
     5941 gggcgaacgc tgttcgaacg ccgtacccgc gccctcgaac tgacggtggc cggggaacgc
     6001 ctgtacgccg tcgtgcgcca ggcactcctt gcggtcgacc gcaccgtgga tgaaatccgc
     6061 ggcgacggcg gcacaccgcg cgtgacgatc accacttatg cgtcgtttgc atccttgtgg
     6121 ctggttccgc gcctgccgga cttccagcga ttgcacccgg ggttcgacat ccgcatcgac
     6181 gcatcggatc gaatggtcga tctcgagtcc gacgacgtcg acctcgccat ccgctggctg
     6241 cggcccggcg caccggtgcc caccgacgcc acgttgctgc agaccgagga atccagtccg
     6301 gcgctcagcc cgcgcctgct ggcgacgcgg aagtcgccgc tgcgggatcc gggtgaactc
     6361 ggaacgctgc cgatcctcgc gatggacgaa tccgtgccca gcgcgcccta cagcagctgg
     6421 gcacgctggt gcgagttcgc tggcattgcg ccgttcgacg gcgccgcgcg gctctacttc
     6481 acgtatgtcg accagtccgt gcaggccgcg gtgcgcggtc agggcgtggc cctggtgcgc
     6541 acaccgttcc tcgacgatct cgtcgcgagt ggtgacctgg tcatgccctt tccccacctg
     6601 cgcatgccga ccggctaccg ctacttcatg atcgtcaatc gcaagcgcag cgcggtcccg
     6661 cacgtggctg catttcgtga ctggatcgtg accgagttcg agctcgggcc tcatcggctc
     6721 acctgaaccg gcacgccggg ctcgttggac acccgcaggc aggccccgtc gggttaaccc
     6781 ggcgtttccg caagccgggg cgctaaaagg tcccggcgtc ggtgatgacg acatcgagct
     6841 gcatgtcgtg tgtctggcgg tcgatcgact cgagccggcc gcattcaaag gcaatcccga
     6901 cacacaaggg ccagggcacg gtctgcgcga gggtgcggtc gtaatagcct ccaccgtagc
     6961 ccagccgata gcgctgcgca tcgaagccca cgcagggaat cagcagggca tcgggctgca
     7021 cggggcggcc atgcgcgggc accggtatgt tgaactcggc ggcccgcatc gat
//
LOCUS       DATIRT010000493         3176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1268431, whole genome shotgun sequence.
ACCESSION   DATIRT010000493 DATIRT010000000
VERSION     DATIRT010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268431"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VLN25_11345"
     CDS             <1..200
                     /locus_tag="VLN25_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11186.1"
                     /translation="QANPAAFGMGNATAPCFVPPTATTAASMCSPNAATQNTYVFWDP
                     FHPTAGTGAIMAQYAITAIGR"
     gene            complement(294..1073)
                     /locus_tag="VLN25_11350"
     CDS             complement(294..1073)
                     /locus_tag="VLN25_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSQ11187.1"
                     /translation="MSIDWAEIAGERLEVLRIAPERDSPPLVFLHEGLGSVAGWRDFP
                     RAVCDRLGAPGMVYSRRGYGRSTPLGRPRGTDYLHREACDVLPALLDHLCITAPILVG
                     HSDGGSIALLFAARYSPRAIAIMAPHVFVEDVTIEGIRVAREAWKHGKLRAPLTRVHD
                     YPDGAFFGWNDGWLDPAFRHWNIEAELPKIRCPVLAIQGIDDQYGTMEQLDRIAAGVQ
                     SPCRLLKLADCGHSPQRDQPQAVIEAIEALYRQSIDPVIKA"
     gene            complement(1087..1656)
                     /locus_tag="VLN25_11355"
     CDS             complement(1087..1656)
                     /locus_tag="VLN25_11355"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HSQ11188.1"
                     /translation="MQALLKQAWWALLVGGLASVIFGVLALMWPGLTLFILAVFFAAT
                     VFVDGIVTMASAVRNRATASHWWVWLLLGLLGVVAGAIGLLSPGTAAGALLLLISAYA
                     IAAGVLMIWAGIKLRAEIEREWLLWLMGGVSVLFGLLLIAQPAAGLMGFVWVIATWAV
                     AEGILKIMLAFKARKFQARLAQAGTATSA"
     gene            complement(1777..2724)
                     /locus_tag="VLN25_11360"
     CDS             complement(1777..2724)
                     /locus_tag="VLN25_11360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HSQ11189.1"
                     /translation="MRALRYAAFGPITDVLRYEDTDAPSTTDDGVVVGTRFVGINPLD
                     WKLVEGQFRLLAKSRPPCGVGAELAGEVISVGSKVGSLRVGDRVAAWLNPFKEPPRAL
                     AEQVAVPAAQCVVIPREVPFDIAAVTPVAGLSALQLCSMIDVQSGQRVLVHGAAGGVG
                     SFLVPMLRDRGATVVATGSARSQSFLRSLQPDAQVDYAAPPSSWLGPFDAVIDCASRL
                     DTAALPALMPGGGHFAVTLPSFPGVIFDPLLNCFRSVRRHTLRLEPNAAQIAQVFALV
                     AEGRVPVSLTQTYAFDEAIAALAESRSGSARGKLAVAIA"
     gene            complement(2733..>3176)
                     /locus_tag="VLN25_11365"
     CDS             complement(2733..>3176)
                     /locus_tag="VLN25_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSQ11190.1"
                     /translation="CLNSARYGIAWGALGAAESCWYTARQYVLDRKQFDRPLAANQLV
                     QKKLADMQTEIALALQGCLRLGRMKDEGTAAVEITSIMKRNSCGKALDVARVARDMLG
                     GNGISDEFGVVRHMLNLEVVNTYEGTHDIHALILGRAQTGIQAFF"
BASE COUNT          569 a         1074 c         1043 g          490 t
ORIGIN      
        1 tgcaggcgaa tcctgcagcc ttcggtatgg ggaacgcgac ggccccgtgc ttcgtgccac
       61 ccaccgccac gacggcggcc agcatgtgtt cgccgaatgc ggcaacgcag aatacctacg
      121 tgttctggga tccgttccat ccgacggccg gcaccggcgc catcatggcg caatacgcga
      181 tcaccgcgat cggccgctga ttcgattggc tgcaaacgtc aacggcgcct gcgggcgccg
      241 ttttcatttc agccgtagcg cagcttcatc aacaggataa acgccagcgg ccctcaggcc
      301 ttgatcacag ggtcgatcga ctgccggtac agcgcctcga tcgcctcgat gaccgcctgc
      361 ggctgatccc gttgcggact gtgcccgcag tcggcgagtt tgagcagacg gcacggggat
      421 tgcacgccgg ccgcaatacg gtcgagctgc tccatcgtcc cgtactgatc gtcgatgccc
      481 tggatggcga gcaccgggca gcggatcttc ggcagttcgg cctcgatgtt ccagtggcgg
      541 aatgcggggt cgagccagcc gtcgttccag ccgaagaacg caccgtccgg gtagtcatgg
      601 acgcgggtca gcggtgcgcg cagcttgccg tgcttccagg cctcgcgcgc gacgcgtatg
      661 ccttcgatgg tcacgtcttc gacgaacacg tgcggtgcca tgatcgcgat cgcgcgaggt
      721 gagtatcgcg ccgcgaacag cagggcgatc gagccgccgt cgctgtgacc caccagtatc
      781 ggtgcggtga tgcaaagatg gtccagcagc gccggcaaca cgtcacacgc ctcacggtgc
      841 aggtaatcgg tgccgcgtgg ccgaccaagt ggcgtcgagc gcccatagcc gcgccgcgag
      901 tagaccatgc ccggcgcgcc gagccggtcg cagactgcgc gcggaaaatc gcgccatccg
      961 gcaacggaac cgagcccttc gtgcaggaac accagcggtg gcgagtcgcg ctcgggcgcg
     1021 atgcggagaa cctcgagccg ttcgccggca atctccgccc agtcgatgct catccggcgg
     1081 ttgggttcat gcgctggttg cggtacccgc ctgcgccaga cgcgcctgga acttgcgcgc
     1141 cttgaacgcc agcatgatct tcaggatgcc ctcggcgaca gcccaggtgg cgattaccca
     1201 tacgaatccc atcagtcccg cggcgggctg cgcaatcagc agcagcccga acagcactga
     1261 tacgccgccc atcagccaca gcagccattc gcgttcgatt tcggcgcgca gcttgatgcc
     1321 ggcccagatc atcagcacgc cagccgcgat cgcgtaagcg gaaatcagca gcagcagcgc
     1381 accggcggcg gtgccgggcg acagcagtcc gatcgcgccg gccacgacgc cgagcagtcc
     1441 gagcagcagc catacccacc aatggctggc agtcgcgcgg ttcctgactg cgctcgccat
     1501 cgtgacgatg ccgtcgacaa agacagtggc cgcgaagaac accgcgagga tgaagagcgt
     1561 caatcccggc cacatgaggg ccagcacgcc aaagatcacg gacgcaagcc cgccgaccag
     1621 caacgcccac cacgcctgct tcagcaatgc ctgcattgtt tccccctgac tacgttcctg
     1681 gatgaccgga gtctgccgcc ccggaaaagg tagcgagcct accaacgccg gccgtcgctg
     1741 ccaattggcg cagggtgggc cgccgacggc ccctgctcag gcgatggcta cggcgagctt
     1801 gccgcgcgcg ctgccgctgc gtgactcggc cagtgcagcg atcgcttcgt cgaaggcgta
     1861 ggtttgggtc aaggaaaccg gcacccggcc ctccgcgacc agcgcgaaca cctgggcgat
     1921 ctgcgctgcg ttgggctcga gccgcagcgt atggcgccgc acgctgcgaa agcagttcag
     1981 cagcggatcg aagatcaccc ccgggaatga cggcagcgtg acggcgaagt ggccgcctcc
     2041 gggcatcagt gctggcagcg ccgcggtatc caagcggctc gcacagtcga tgaccgcatc
     2101 gaagggcccg agccaggatg acggcggcgc ggcgtagtcg acctgtgcgt cgggctgcaa
     2161 tgagcgcagg aacgactggc tgcgcgcgct tccggtcgcg acgacggtgg cgccccggtc
     2221 gcgcagcatc ggcacgagga agctgccgac gccgccagcg gcaccgtgga ccagcacgcg
     2281 ctgtccggac tgaacgtcga tcatcgagca taactgcaag gcagacagtc cggccaccgg
     2341 cgtgacggca gcgatgtcga aaggcacctc acgcggaatc accacgcact gggctgcagg
     2401 taccgcgacc tgctcggcca gcgcgcgtgg cggttccttg aagggattaa gccaggcggc
     2461 gacccgatca cccacgcgca gggatcccac ctttgaaccg acgctgatca cttcgccggc
     2521 aagctcggct ccgacgccgc acggcggccg gctctttgcc agcagcctga actgaccctc
     2581 gaccagcttc cagtcgagcg ggttgatgcc cacgaagcgc gtgccaacca cgaccccatc
     2641 gtccgtcgtc gacggtgcgt cggtgtcctc gtatcgaagg acatcggtga tcgggccgaa
     2701 tgcggcgtag cgcagggcgc gcatgcggcg aatcagaaaa atgcctggat gccggtttgc
     2761 gcgcgcccga ggatcagtgc atggatgtcg tgcgtgcctt cataggtatt gacgacctcg
     2821 aggttgagca tgtggcgcac gacgccgaac tcgtcgctga tgccgttgcc gccgagcatg
     2881 tcgcgggcca cacgcgcgac gtcgagcgcc ttgccgcagg aattgcgctt catgatgctg
     2941 gtgatctcga cggcagccgt gccttcgtcc ttcatgcgac cgagccgcag gcagccctgc
     3001 agggccagtg cgatctcggt ctgcatgtcg gcaagcttct tttgcaccag ctggttggcc
     3061 gcgagcgggc ggtcgaactg cttgcggtcg agcacgtact ggcgcgcggt gtaccagcag
     3121 gactcggcgg cgccgagcgc gccccaggca atgccgtagc gcgccgagtt caggca
//
LOCUS       DATIRT010000494         6212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1072063, whole genome shotgun sequence.
ACCESSION   DATIRT010000494 DATIRT010000000
VERSION     DATIRT010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6212
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072063"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VLN25_11370"
     CDS             complement(<1..665)
                     /locus_tag="VLN25_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSQ11191.1"
                     /translation="MKFALKIIAVAAAALTAGTTLAQDIKVGVTLSATGPAASLGIPE
                     RNTFALVPQTIGGQKVQYIVLDDASDTTTAVKNARKLITEDKVDVLIGSTITPNSLAM
                     IDVAAESETPMVSMAASSRIVEPVDAKRRWVFKTPQNDQQMALVIVGDMINRGVKTVG
                     FIGFADAYGEGWWNEFSKIAETRGLKVLASERYNRADTSVTGQVLKLMASKPDAILIA
                     GAGT"
     gene            complement(754..1953)
                     /gene="pcaF"
                     /locus_tag="VLN25_11375"
     CDS             complement(754..1953)
                     /gene="pcaF"
                     /locus_tag="VLN25_11375"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HSQ11192.1"
                     /translation="MTDAFLCDGVRTPIGRYGGALAVVRTDDLAAVPLRALIARHPKV
                     DWAALDDVYFGCANQAGEDNRNVARMAVLLAGLPSEVPAATVNRLCGSSMDAVASCAR
                     AIRAGEAELMIAGGVEGMSRAPFVMPKADSAFSRANAVYDTTLGWRFINPAFKQQFGV
                     DSMPETAENVAAEFGIARGDQDMFALRSQQRYAAARAKGYFAGEVVPVTIPGRKGDIV
                     VGDDEHPRETTLEALGKLKGVVRPDGTVTAGNASGINDGSVALLIGSEAAAARHGLTP
                     LVRIVGAATAGVPPRIMGIGPAPATKKLLARYSLTIAQVEVIELNEAFAAQALAVTRQ
                     LGLADDAPYVNANGGAIAIGHPLGASGARIVLTAARQLIAGGGRYAVATMCIGVGQGI
                     ALLVERV"
     gene            complement(1950..2651)
                     /locus_tag="VLN25_11380"
     CDS             complement(1950..2651)
                     /locus_tag="VLN25_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSQ11193.1"
                     /translation="MARTKAAGFEAQRGAIRDAAAQLFAEKGYASASIADIAAACGVS
                     KALLYHYYRDKEHLLHDIAESYVERLQAIVEEVRGEALSPPARMRQLITRFMHEYEHS
                     AARHRVLVQDVKYLSRAHRSRIVARQRGVVDGFAQVIGELAPAAAVSALRKPLTMILF
                     GMMNWTFTWFSDRGPLRYDDLAPVVADLFLGGLGRIHAVDAGTAIAVPVAARVTAPVA
                     ARRPRTRTQSIKAIA"
     gene            complement(2654..3721)
                     /gene="paaE"
                     /locus_tag="VLN25_11385"
     CDS             complement(2654..3721)
                     /gene="paaE"
                     /locus_tag="VLN25_11385"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014631141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HSQ11194.1"
                     /translation="MTPRFHSLRIAEVRRETQDCVSLRFEVPGDIADDYRFVQGQHLA
                     LRTRFEGEELRRSYSICAGCDDGELRVAVKKVPGGRFSVWVNERLKPGDAIDVMTPEG
                     RFFTPLAAEHDRHYVAFAAGSGITPVLSLISTTLAREPRSRFTLVYGNRRQANVMFHE
                     ALEDLKDRYLTRFRLYNVFSREEQDVEIFNGRIDAAKVRQFLETLIPSTTIDEAFVCG
                     PASMIDEVEQALIAAGIGRDHVHVERFGVPGAAAAVPIDDAEAAEAKLTLIIDGVKRE
                     VDFHRGQHSILEAGRAAGLDLPYSCKGGMCSTCRAKLLDGQVKMAKNYALEPHEVAAG
                     YVLTCQSYPLTERVLISYDER"
     gene            complement(3804..4301)
                     /gene="paaD"
                     /locus_tag="VLN25_11390"
     CDS             complement(3804..4301)
                     /gene="paaD"
                     /locus_tag="VLN25_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HSQ11195.1"
                     /translation="MTGTSITAVQAWTILGRVPDPEIPVISVTELGIVRDVQVTDDVV
                     DVVVTPTYSGCPATEVIERSIREALTAAGASEVRVTTQLAPAWTTDWIEPQAREKLRI
                     YGIAPPGTRGASQEQPLQFVPRIHCPRCGSADTERLSQFGATACKALYRCRSCREPFE
                     YFKPL"
     gene            complement(4298..5068)
                     /gene="paaC"
                     /locus_tag="VLN25_11395"
     CDS             complement(4298..5068)
                     /gene="paaC"
                     /locus_tag="VLN25_11395"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HSQ11196.1"
                     /translation="MSTTTTAHVAHIDYVLHIADTSLILGQRLSEWCGHGPVIEEDIA
                     LTNIALDLIGQARLLLSHAGKLERGGRDEDQLAFLRTEPQYRNLAIVELPNGDFGRTI
                     LRNFLVAAWQQSLWQRLTTSSDAELAAIAAKSLKETRYHLQHASDWVVRLGDGTDESH
                     ARMKAALDTLWPYTTEFFSATAGDPVLEAAAIGPAWSTLEPAWDSVVRPVLEVATLSA
                     PVRTPFISRGKLGQHTEYMGHLLTEMQYLQRTYPGGTW"
     gene            complement(5076..5369)
                     /gene="paaB"
                     /locus_tag="VLN25_11400"
     CDS             complement(5076..5369)
                     /gene="paaB"
                     /locus_tag="VLN25_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HSQ11197.1"
                     /translation="MTESSRQWPLWEVFIRSKNGLDHKHCGSVHAPDAKMALGNARDV
                     YTRRMEGVSIWVVRADQIVASDPDAKPEMFDPAEDKIYRHPTFYKLPDEVNHM"
     gene            complement(5499..>6212)
                     /gene="paaA"
                     /locus_tag="VLN25_11405"
     CDS             complement(5499..>6212)
                     /gene="paaA"
                     /locus_tag="VLN25_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HSQ11198.1"
                     /translation="HGMYLYAAAETLGVARDDLVDALHSGKAKYSSIFNYPTLNWADI
                     GVIGWLVDGAAIMNQIPLCRCSYGPYARAMIRICKEESFHQRQGYDLLLTMMKGTADQ
                     RAMVQDAVNRWWWPSLMMFGPHDKDSVHSGDSIKWGIKRISNDDLRQKFVDATVPQAK
                     VLGISLPDPDLRFNEERGHWDFGTIDWSEFWNVVNGHGQCNRDRLADRVQAWEDGAWV
                     REAALAHADKQRRRDRLAA"
BASE COUNT         1038 a         2061 c         1997 g         1116 t
ORIGIN      
        1 gtacctgctc cggcgatcag aatcgcatcc ggcttggaag ccatcagctt gagcacctgt
       61 ccggtcaccg acgtgtcggc gcggttgtaa cgctcgctcg ccaggacctt cagcccgcgc
      121 gtttcggcga tcttgctgaa ctcgttccac cagccttcgc cgtaggcgtc tgcaaagccg
      181 atgaagccga cggtcttcac gccgcggttg atcatgtcgc cgacgatgac cagcgccatc
      241 tgctggtcgt tctgcggtgt cttgaacacc catcgccttt ttgcgtcgac tggctcgacg
      301 atgcgactcg acgccgccat cgagaccatc ggtgtctcgc tctcggccgc aacatcgatc
      361 atcgccagcg agttcggcgt gatcgtcgac ccgatcagca cgtcgacctt gtcctcggta
      421 atgagcttgc gtgcattttt caccgccgtc gtggtgtcgg aggcgtcgtc cagcacgatg
      481 tactgcacct tctggccgcc gatggtctgc ggcaccagcg cgaaggtgtt gcgctcgggg
      541 atgccgagcg aagcagccgg cccggttgcc gacagcgtga cgccgacctt gatgtcctgt
      601 gccagcgtgg tgccggcggt gagcgcagcc gcagcgacgg caatgatctt cagagcgaac
      661 ttcatgtgtc tcctcgtctt cgttgaacgc gtcctcgccg tggtgtcggc gatggtcgcg
      721 gttggaaatt ctcaaacccg catcacaaca aactcacacc cgttcgacaa gaagggcgat
      781 cccctgcccg actccgatgc acatcgtcgc cacggcataa cgaccgccac cggcgatgag
      841 ctgccgcgcg gcggtgagca cgatgcgcgc gccggacgcg ccgagcggat ggccgatcgc
      901 gatcgctccg ccgttggcgt tcacatacgg tgcatcgtcg gccaagccga gctggcgagt
      961 gaccgcgagc gcctgggctg caaacgcttc gttgagctcg atgacctcga cctgggcgat
     1021 cgtcaggctg taacgagcga gcagcttctt cgtggccggc gccggcccga tgcccatgat
     1081 gcgcggaggc acgccggccg tggccgcgcc gacgatacgt accagcggcg tcagtccgtg
     1141 gcgcgcagcc gccgcctcgc tgccaatcag cagtgcgacc gaaccatcgt tgatgcctga
     1201 cgcgttgccc gccgtcaccg tgccgtccgg acgaacgacg cccttcagtt tgccaagcgc
     1261 ctcgagagtg gtctcccgcg gatgttcatc gtcacctacc acgatgtcgc ccttgcggcc
     1321 cggaatcgtg accgggacga cttccccggc gaagtagccc ttcgccctgg cggcggcata
     1381 gcgctgctgc gaccgcaacg caaacatgtc ctggtcacct cgggcaatgc cgaactccgc
     1441 ggcaacgttc tcggcggtct cgggcatcga gtcgacgccg aactgctgtt tgaacgccgg
     1501 gttgatgaaa cgccacccga gcgtggtgtc gtagaccgca ttcgcccgcg aaaaggcact
     1561 gtcggccttg ggcatcacga agggcgcccg cgacatgccc tcgacgccgc ccgcgatcat
     1621 cagctcggcc tcgccggcgc ggatcgcgcg ggcgcagctg gccacggcgt ccatgctcga
     1681 gccgcacagc cggttgaccg tcgccgccgg aacctcggac ggaaggcccg ccagcagcac
     1741 ggccatgcgc gcgacgttgc ggttgtcttc gccggcctgg tttgcacagc cgaagtacac
     1801 atcgtccagt gcggcccagt cgaccttcgg atggcgcgcg atcagcgcac gcaggggcac
     1861 tgctgccagg tcatcggtgc gcaccacggc cagcgcaccg ccgtagcggc cgatcggcgt
     1921 gcgcacgcca tcacagagaa atgcatcggt catgcgatcg ccttgatgga ttgtgtccgc
     1981 gtgcgcggac gccgcgctgc caccggtgct gtgacccgtg ctgcaaccgg cactgcgatg
     2041 gctgtcccgg catccaccgc atgaatccgg ccgaggccac cgaggaagag atccgccacg
     2101 accggcgcga gatcgtcata gcgcagcggg ccgcgatcgc tgaaccaggt gaacgtccag
     2161 ttcatcattc cgaacaggat catcgtcagc ggcttgcgca gcgcagagac ggcggctgcc
     2221 ggcgcgagct cgccaatgac ctgcgcgaaa ccgtcaacca cgccgcgctg gcgggcgacg
     2281 atgcgactgc gatgtgcgcg cgagaggtac ttcacgtcct gcaccagcac gcgatggcgc
     2341 gccgccgagt gctcgtactc gtgcatgaaa cgcgtgatga gctgtcgcat gcgcgcagga
     2401 ggcgaaagcg cttccccccg cacttcctcg acgatcgcct gcagccgctc gacgtagctc
     2461 tccgcgatgt cgtgcagcag gtgttccttg tcacggtaat agtgataaag cagtgccttg
     2521 gacacaccgc acgcggccgc gatgtcggca atcgaggccg aggcgtagcc cttctcggcg
     2581 aagagctgcg ccgccgcatc gcggattgct ccgcgctgcg cttcgaaacc cgccgccttg
     2641 gtccgcgcca cgtctatctt tcgtcgtagg agatcagcac ccgctcggtc agcgggtagg
     2701 actggcaggt cagcacatag ccggccgcca cttcatgcgg ctcaagcgcg tagttcttcg
     2761 ccattttcac ctggccgtcg agcagcttgg cgcggcaggt gctgcacatg ccgcccttgc
     2821 acgagtacgg aaggtcgagc cccgctgcgc gccccgcctc gaggatcgaa tgctggccgc
     2881 ggtgaaaatc gacctcacgc ttgacgccgt cgatgatcag cgtgagcttg gcctcggccg
     2941 cctcggcgtc gtcgatcggg accgcggccg cggcccccgg cacgccgaag cgttcgacgt
     3001 gcacatggtc gcggccgatg ccggctgcaa tcagcgcctg ctcgacttcg tcgatcatcg
     3061 atgccggtcc gcagacgaac gcttcgtcaa tcgtcgtgga cgggatcagc gtctcaagga
     3121 attgccgcac cttggccgcg tcgatccgac cgttgaagat ctcgacgtcc tgctcctcgc
     3181 gcgagaacac gttgtagagg cggaagcgcg tcagatagcg gtccttcaga tcttcgagtg
     3241 cctcatgaaa catcacgttc gcctgccggc gattgccgta cacaagcgtg aagcgagagc
     3301 gcggctcgcg cgccagcgtc gtgcttatca gtgacagcac gggcgtgatg ccgctgcccg
     3361 cagcgaacgc cacgtagtgg cggtcgtgct ccgcggccag cggcgtgaag aagcggccct
     3421 cgggcgtcat cacgtcgatt gcatcgcccg gtttcagccg ctcgttgacc cacaccgaga
     3481 aacgcccgcc cggtaccttc ttgacggcta cacgcagttc gccatcgtcg cagccagcgc
     3541 agatcgagta cgagcgacgc agctcctcgc cctcgaaacg ggtgcgcagc gcgaggtgct
     3601 ggccctggac gaagcgatag tcgtcggcaa tgtcgcccgg aacctcgaac cggaggctga
     3661 cgcagtcctg ggtttcgcgc cggacttcgg cgatgcgcag ggaatggaag cgaggagtca
     3721 tcgcgattcg attttgtgga gcttggtcaa aggcgctcgt tcaacgacgt cgattcaaag
     3781 tagcttgtta aaaaagggct ggttcaaagg ggcttgaagt attcgaacgg ttcgcggcag
     3841 gagcggcacc gatagagcgc cttgcaggcg gtggcgccga actgcgacag gcgttccgta
     3901 tcggcactgc cgcagcgcgg acagtggatg cggggcacga actgaagcgg ctgctcctgg
     3961 ctcgcgcctc tggtgcccgg cggagcgata ccgtagatgc ggagcttttc ccgggcttgc
     4021 ggctcgatcc agtccgtggt ccatgcgggc gccagttggg tcgtgacccg cacctcggat
     4081 gcgccggccg cagtcagcgc ttcgcgaatc gagcgctcga tcacctccgt tgccgggcat
     4141 cccgagtagg tcggtgtcac cacgacatcg accacatcat cggttacctg cacgtcgcgc
     4201 acgatgccga gctcggtcac ggaaattacc gggatctcgg gatcggggac gcggccgagg
     4261 atggtccagg cctgcacagc agtgatcgaa gtgcccgtca ccacgtgccg cccggatagg
     4321 ttcgttgcag atactgcatt tcggtcaaca aatggcccat gtactcggtg tgctgaccca
     4381 gcttgccgcg cgaaatgaac ggcgtccgca cgggtgccga cagcgtcgcg acttcgagca
     4441 ccggtcgcac gacgctgtcc caggccggtt cgagagttga ccaagcgggc ccaatcgcgg
     4501 ccgcttcgag gaccgggtcc ccggccgtgg cgctgaagaa ctccgtggtg tacggccaga
     4561 gcgtgtcgag ggcggccttc atgcgggcgt gcgactcgtc ggtgccatca cccaggcgca
     4621 cgacccagtc cgaggcgtgc tgcaggtgat agcgggtctc cttcaggctc ttcgccgcga
     4681 tcgccgcgag ctcggcgtcg cttgacgtcg tgagccgctg ccacagcgac tgctgccacg
     4741 cggcaacaag gaagttgcgc aggatcgtgc ggccaaagtc gccattgggc aattcgacga
     4801 tcgccaggtt tcgatactgc ggctcggtgc gcaggaacgc gagctggtcc tcgtcgcgac
     4861 cgccgcgttc cagcttgccg gcatgagaca gcaacagacg cgcctggccg atcaggtcca
     4921 gcgcgatgtt ggtgagcgcg atgtcttcct caatcacggg gccgtgacca caccattccg
     4981 acaggcgctg gccgagtatc agcgacgtgt cggcgatatg aaggacgtaa tcgatgtggg
     5041 ccacgtgggc cgttgtcgtt gtgctcatcg cggactcaca tgtgattcac ttcgtcaggg
     5101 agcttgtaaa acgtcgggtg ccggtagatc ttgtcctccg ccggatcgaa catctcaggc
     5161 ttggcgtccg gatcgctcgc tacgatctga tcggcccgca ccacccagat gctgacgccc
     5221 tccattcgcc gcgtgtagac gtcacgcgcg ttgcccagcg ccatctttgc gtccggggca
     5281 tgcacgctgc cgcaatgctt gtggtcgagg ccgttcttgc tgcggataaa cacttcccag
     5341 agaggccatt ggcggctgga ttcggtcatt tcggatcctc ttgattattt ccttcggtcg
     5401 acgccgaagc ttcgttcggc gccaatggcc cgcgggctgt atgaggacgc aagggggcgg
     5461 cgcccccttg gaaccctgct gaaaacagga ggcacatatc aggcggcgag ccggtcgcgg
     5521 cggcgctgtt tgtcggcgtg cgcgagtgcg gcttcgcgca cccaggcgcc gtcttcccag
     5581 gcctgcacgc ggtcggcgag tcggtcgcgg ttgcactggc cgtggccgtt gacgacgttc
     5641 cagaactcgc tccagtcgat cgttccgaag tcccagtgcc cgcgctcctc gttgaaacgc
     5701 aggtcgggat cgggcagcga gatgccgagc accttcgcct gcggcaccgt ggcgtcgacg
     5761 aacttctgcc gcaggtcgtc gttgctgatg cgcttgatgc cccacttgat cgagtcgccc
     5821 gaatgcacgg agtccttgtc gtgcgggccg aacatcatca gcgacggcca ccaccaccgg
     5881 ttcaccgcgt cctgcaccat cgcgcgctgg tcggccgtgc ccttcatcat cgtcagcagc
     5941 aggtcgtagc cctggcgctg gtggaacgac tcttccttgc agatgcggat catcgcgcgc
     6001 gcatacgggc cgtacgaaca gcggcacaaa gggatctggt tcatgatcgc cgcgccgtct
     6061 accagccagc cgatcacgcc gatgtcggcc cagttcagcg tcgggtaatt gaagatcgac
     6121 gagtacttcg ccttgcccga gtgcagcgcg tcgaccaggt cgtcgcgcgc cacgccgagc
     6181 gtctcggcgg cggcgtacag gtacatgccg tg
//
LOCUS       DATIRT010000495         2828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_383390, whole genome shotgun sequence.
ACCESSION   DATIRT010000495 DATIRT010000000
VERSION     DATIRT010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383390"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VLN25_11410"
     CDS             <1..401
                     /locus_tag="VLN25_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11199.1"
                     /translation="ALRPEPAPPAPVVNAVPVPVTPAPPPVADAPPESRTAPSEDLKP
                     AETKAAMRRRSESASTAVPVRDGTVNLAVTPWGEVFVDGVARGVSPPLTQLSLSPGAH
                     TIEIRNSSAPPLSVRVELRSGEAISLQHRF"
     gene            577..2109
                     /locus_tag="VLN25_11415"
     CDS             577..2109
                     /locus_tag="VLN25_11415"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11200.1"
                     /translation="MSQTVASKSPTLSPNLKLVGRYSVLRELRRDRYTTVYLAFDPVL
                     NRELVVKAVQLHPASGEDRAAHERVEQAFMRQAQAAGRLHHPHIVTVFDAGHVNRIGY
                     IALEKVDGKMLDELLTEGYRPGFLQAADIVARVSDAIEFAHTRAIPHGHLSPSRIYLQ
                     GPERTPKVMGFGGWIDSGMTGDFELHGTDRLLPYFGDELSGEARRKDIRALGALLFLL
                     LTGTRPDVKTLRAQGKAIESPILQLKPNAPLALAEIAEQALELGGTRVFSTAAQVRNA
                     LTTYLWGNRGEMPLPGSATITGMPARLSVMPQRAATMTVATIVATPARSEPARERRSR
                     GRMHVVAGLVGAVAIAAIAGIGMQANLFGGRSMMTSPARDTMASPPVARPAPMTDTAS
                     AAAPAPTHPPADGAVRGTVPGTTASDVAQGAVRTPPARSTPARAAVVNGALGVVALAV
                     APWGTVYVNGDARGTTPPLTQLTLPAGRYTIEIRNGERTPYIAQLDVTPERPQQIRHR
                     FQ"
     gene            2106..2573
                     /locus_tag="VLN25_11420"
     CDS             2106..2573
                     /locus_tag="VLN25_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TssQ family T6SS-associated lipoprotein"
                     /protein_id="HSQ11201.1"
                     /translation="MNDPAARTLLRCAAPLAIAALSGCAAFAPPPPPPPAPPPTISIA
                     RLYDQPAERAFISGLRYYEEGQYERAETLIKRALAEGLRDPYDVAVAQKHLAFIACAY
                     NRPTDCEQAFRSAWAADPNFRLTEAEVGHPLWGPVYKRVAAEQVPKPPPAASK"
BASE COUNT          459 a         1028 c          885 g          456 t
ORIGIN      
        1 ttgcgctgcg tccggaaccc gcgccaccgg cccccgtggt caatgccgtc ccggtgcccg
       61 tgacaccggc tccgccgccg gtggcggacg cgccgcccga gtcccgcacg gccccgtccg
      121 aggacctcaa gcctgcggag accaaggcgg cgatgcggcg gcgctccgag tcagcctcga
      181 cagcagttcc cgtgcgcgac ggcacagtga acctggcggt caccccttgg ggagaagtat
      241 ttgtcgacgg cgtcgcgcgc ggtgtgtcgc cgccgctgac gcagctttca ctgtcacccg
      301 gtgcccacac aatcgaaatc cgcaacagtt cggcgccgcc gctttccgta cgcgtcgaac
      361 ttcgctccgg cgaagcgatt tctctgcaac atcgcttcta gggccgatgg caggcggccc
      421 gacctgtcat agggaagacg attgaccccc ttttcctccg tttgcagcgg ttgagtccat
      481 tgcgctcatc ggctagcctc aacgggccat gattcggacc gccgactcgg atcgcgcgtg
      541 tttgctgcac gccacgccga ccgtgggcgc aggctgatgt cgcagaccgt tgcatcgaag
      601 tcgccgaccc tgtcgcccaa cctcaaactg gtcggtcgct attcggtgct gcgcgagctg
      661 cgccgcgatc gctacaccac cgtctatctc gccttcgacc ccgtcctgaa ccgcgagctc
      721 gtggtgaagg cggtgcagct gcacccggcc agcggcgagg accgcgctgc ccatgagcgc
      781 gtcgaacagg ctttcatgcg tcaggcccag gcggccgggc gtctgcatca tccgcacatc
      841 gtcaccgttt tcgacgccgg ccatgtcaat cggattggct acatcgcgct cgagaaggtc
      901 gacggcaaga tgctcgacga actgctcacc gagggctacc ggccgggatt cctgcaggcg
      961 gccgatatcg tcgcgcgcgt ctctgacgcg atcgaattcg cgcatacgcg tgccattccg
     1021 cacggccacc tgtcgccgtc ccgcatttac ctgcagggac cggagcgcac gcccaaggtg
     1081 atgggattcg gcggctggat agacagcggc atgactggag acttcgagct gcatggcacc
     1141 gaccggctgc tgccgtactt cggcgacgag ctgtccggcg aggcacgccg caaggacatc
     1201 cgcgcgctgg gtgcactgct cttcctgctt ctgaccggaa cccgcccgga tgtgaagacc
     1261 ctgcgtgcgc aaggcaaggc gattgagagt ccgatactgc agctcaagcc caatgcgccg
     1321 ctcgcgctcg ccgagattgc cgagcaggca ctcgagctgg gcggaacccg cgtgttttcc
     1381 accgcggcgc aagtgcgcaa cgcgctgacg acctatctgt ggggcaaccg cggcgaaatg
     1441 ccgctgccgg gttccgcaac gatcaccggc atgccagccc gcctgtccgt gatgccacag
     1501 cgggccgcca cgatgaccgt ggcgacgatc gtcgccacgc cggcacgctc cgaaccggcg
     1561 cgggagcgcc gcagccgtgg gcgcatgcac gtcgtcgcgg gcctggtcgg tgccgtcgcc
     1621 atcgctgcga ttgccgggat cgggatgcag gcgaacctct tcggcggtcg atccatgatg
     1681 acgagtccgg cccgcgacac gatggccagc ccgccggtcg cgaggccggc acccatgacg
     1741 gacacggcct ccgcggccgc gccagcgccg acccatccac cggccgatgg tgcagtccgc
     1801 ggcacagtcc cgggcaccac agcgtcagac gttgcacagg gcgctgttcg cactccgccc
     1861 gcccgctcga cgcctgcgcg cgctgctgtg gtcaacggtg ccctgggcgt cgttgcactc
     1921 gccgttgccc cgtggggcac cgtctatgtc aacggcgatg cgcgcggcac gacgcctcca
     1981 ctgacgcaac tgacactgcc cgcgggccgc tacacgatcg agattcgcaa tggcgaacgc
     2041 acgccgtaca ttgcacagtt ggatgtcacg cccgagcgtc cgcagcagat ccggcatcga
     2101 ttccaatgaa cgatccggcg gctcgcacac tgctccgatg cgccgcgcca ctggccatcg
     2161 ctgccctgag cggctgtgct gcgtttgcgc cgccaccgcc tccgccgccc gcaccgccgc
     2221 cgaccatcag catcgcccgt ctgtatgacc agccggcgga gcgagccttt atcagcggcc
     2281 tgcgctatta cgaagaaggc cagtacgagc gtgccgaaac gctcatcaag cgcgcactgg
     2341 ccgaaggtct gcgtgacccg tatgacgttg cggtggcaca gaagcatctt gccttcatcg
     2401 cgtgcgccta caaccgcccc acggactgcg agcaggcgtt ccgctctgcc tgggccgccg
     2461 acccgaactt caggctcacc gaagcggagg tcggacatcc gctctggggc ccggtctaca
     2521 agcgagttgc cgccgagcag gttcccaaac ccccacccgc cgcctcgaag taacgcagcc
     2581 ggccagccag cctttgattc acgagcgggc gctgggccgt cccaatcccg ttcgtcccat
     2641 ctgatcggtc cgagtgcacg tgcgagccga aggctcgcgt acacgaggac cgcccactag
     2701 aacccgatac gcgccttctt tgcagcacga tcttcaacga catcgtcggg ccggacctcg
     2761 ctgcgaccgt cgagcttggc actgccgaag gccgccagca gcacgcggcg catttcgcgt
     2821 ggcttcag
//
LOCUS       DATIRT010000496         6504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1072233, whole genome shotgun sequence.
ACCESSION   DATIRT010000496 DATIRT010000000
VERSION     DATIRT010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6504
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072233"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VLN25_11425"
     CDS             complement(<1..536)
                     /locus_tag="VLN25_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HSQ11202.1"
                     /translation="MSTQGRPMSALMTVAELARTVAAQDVRGDGRIGFASVSTDTRTL
                     PNGALFVALRGERFDGHDYAQSAADRGAAALLVEHAVEAAVPQIIVADTKHALGLGAA
                     QWRARFTLPVIAVTGSNGKTTVTQMIAAILAQSFGERHRLATRGNLNNEIGLPLMLWQ
                     LGSQHKAAVFELGMNHPG"
     gene            complement(533..2065)
                     /locus_tag="VLN25_11430"
     CDS             complement(533..2065)
                     /locus_tag="VLN25_11430"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HSQ11203.1"
                     /translation="MTMVAATPSTAMSPCVRDALAFLHELPLASGERDLRLDSRAVRA
                     GDIFLAVPGGRSDGRQFLADAIDRGAAAALVDADGWDGAAQVIPVLPVNGLRDSLGAL
                     AAAYYERPSEQLLSIGVTGTNGKTSCSQWIAQLLTHEGRRCAVVGTVGIGFLGSLHAS
                     ELTTPDPVSLQRELRHLLDAGAQALAMEVSSIGLEQRRVDGMHFDVALFTNLTRDHLD
                     VHGTMERYEAAKAMLFEWPGLRHAVINLDDPAGCRVAARAADRGVDVIGYSAQADTTP
                     RPLAPRLLARQIRATEEGLAFELCVGDQTHPVEVQLVGHFNVSNLLGVVGVSLACGIR
                     SDVAAAALPTMVPPPGRMQRVDANVQPLVVVDYAHTPDALAQALEALRPLVRARQGRL
                     WVVFGAGGDRDPGKRAPMGAAAAAADAVIITSDNPRTEDPQHIVDQVAAGARAARNLL
                     TEVDRARAIERAIIAADPADVVLIAGKGHEDYQIVGTEKRPFSDVVQARRSLTMRARD
                     RA"
     gene            complement(2062..3855)
                     /locus_tag="VLN25_11435"
     CDS             complement(2062..3855)
                     /locus_tag="VLN25_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HSQ11204.1"
                     /translation="MKGNSRSSNARPARRSESGASRRERVAFNTNPLLTVALPAWRSK
                     LLLFMLFVGFVTLGGRAFYLMGGVTTDFLQRQGEARYARTLEIPATRGRVTDRNGIVL
                     ASSVPARAVWAIPDDVVATPAQVADLARLLGMNPAEVKRRLENEDRSFVYLRRQVDVD
                     VADRIAALKISGVHSSREFKRHYPEGPTSAHLVGFTNVEDRGQEGIELAHESTLAGRA
                     GSRRVIKDRLGRVVEDDWLREPVDGRDLALSIDNRVQYIAFSALKGAIDKHQAKAGAV
                     IVLDVASGEVLALANWPSYDPNARGRLAPDAIRNRVITDTFEPGSTMKPFAVATALEL
                     GKVRPDTRIQTAPGRMTIGDRTIGDAHAHGLLTVEEVVAKSSNVGTAKIALDLPAQSL
                     WDSYTALGFGQAPRMGFPGAVAGRLRPAKTWRPIEQATISYGHGVSVTLVQLARAYAA
                     LARDGELVPLTLTKSEVSPIAVRVLSVETARAMRKMLEMAVADDGTAPAARIAGYRVA
                     GKTGTAHKLKNGQYVKEYVASFVGFAPVSDPRIVIAVMVDEPSGGAYYGGQVAAPVFA
                     QIAGASLRTLQVAPDGPLEVPKQLLALRQAR"
     gene            complement(3852..4160)
                     /gene="ftsL"
                     /locus_tag="VLN25_11440"
     CDS             complement(3852..4160)
                     /gene="ftsL"
                     /locus_tag="VLN25_11440"
                     /inference="COORDINATES: protein motif:HMM:NF016860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HSQ11205.1"
                     /translation="MIRHVLVYFAVVLLFASSLGLVTAQHRTRSLFVDLERAQQDAKQ
                     LDVNYERLTIELARLAQPAAVERAALRLGFKAAEASRTVFLNLPQASATTPVAPGARK
                     "
     gene            complement(4157..5143)
                     /gene="rsmH"
                     /locus_tag="VLN25_11445"
     CDS             complement(4157..5143)
                     /gene="rsmH"
                     /locus_tag="VLN25_11445"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HSQ11206.1"
                     /translation="MTPVPSTDARGHEAVLAVPAVALLVTDPRATYVDATFGRGGHAR
                     LILERLAPTGRLIAFDRDPAAVAAAADIRDPRLHIEHTAFSHLGETLAALGVTSAQGV
                     LLDIGVSSPQIDTAERGFSWRSDGPLDMRMDTTRGETAAQWLARASIDELTGVIRSYG
                     EERFASSIAKAVVARREAGRPVSTTGDLAALVAGAIPRKSRTDGTQHPATRTFQAVRI
                     HINQELEELALALEQSVQVLATGGRLVVISFHSLEDRIVKRFIDAHAHPDRAAPGTRA
                     LPLRAAELPAPRLAAITRVRADDAEVAVNPRARSAVMRVAERTAAAWPGAAR"
     gene            complement(5150..5578)
                     /gene="mraZ"
                     /locus_tag="VLN25_11450"
     CDS             complement(5150..5578)
                     /gene="mraZ"
                     /locus_tag="VLN25_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HSQ11207.1"
                     /translation="MFQGASHLTLDGKGRLSMPARHRDALAASCEGRLTLTRHPDGCV
                     LIYPRPTWEVRRQQIAQLPYAARALQRLLLGSAVDIELDSAGRILVTAELRSAAGLDR
                     EVMLLGMGAHFELWDVALLGEHERQSLANGLPDSAADFTF"
     gene            complement(6031..6337)
                     /gene="rnpB"
                     /locus_tag="VLN25_11455"
     ncRNA           complement(6031..6337)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VLN25_11455"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1109 a         2202 c         2095 g         1098 t
ORIGIN      
        1 tccccgggat gattcatgcc gagttcgaac acagccgcct tgtgctgcga gccgagttgc
       61 cacagcatca gcggcaggcc gatctcgttg tttaggttgc cgcgcgtggc gagtcgatgc
      121 cgctccccga acgactgcgc aaggatcgcg gcgatcatct gggtgaccgt ggtcttgccg
      181 ttgctccccg tcacggcaat caccggcagc gtgaagcgcg cccgccactg cgccgcgccc
      241 agcccgagcg cgtgcttggt gtccgcaaca atgatctgcg ggactgcggc ttcgacggcg
      301 tgttccacca gaagcgcggc ggcaccgcga tcagcggccg attgcgcata gtcgtgcccg
      361 tcgaagcgtt cgccgcgcaa cgccacgaac agggctccgt ttggcagcgt gcgcgtatcg
      421 gtcgatacgc tcgcaaagcc gatgcgtcca tccccacgca cgtcctgcgc cgccaccgtc
      481 cgcgccagct cggcgaccgt catcagtgcg ctcatcgggc gaccttgcgt gctcatgccc
      541 ggtcccgcgc acgcatcgtc aggctgcggc gcgcctgcac cacgtcggaa aacgggcgct
      601 tctcggtgcc gacgatctgg tagtcctcgt gcccctttcc ggcgatcagc accacgtcgg
      661 caggatccgc tgcgatgatg gcgcgctcga ttgcgcgcgc acggtcgacc tcggtcagca
      721 gattgcgggc cgctcgtgcc ccggcggcaa cctggtcgac gatgtgctgt ggatcctcgg
      781 tccgcgggtt atcgctggtg atgatgacgg cgtccgcagc cgccgccgcc gcgcccatcg
      841 gcgcgcgctt gccaggatca cgatcaccgc cggccccgaa cacgacccat agccggccct
      901 ggcgcgccct taccaggggt cgaagcgcct cgagcgcctg ggccagcgca tccggcgtgt
      961 gcgcgtaatc gacaacgacc aggggctgca cgttcgcgtc gacacgctgc atgcgccccg
     1021 gtggcggcac catggtcggc aacgcagcag ccgcgacgtc gcttcggatg ccacatgcaa
     1081 gcgacacacc gacgacaccg agcagattgg acacattgaa gtgcccgacc agttgaacct
     1141 cgaccggatg cgtctgatca ccgacacaca gttcgaatgc cagtccctct tccgtggcac
     1201 ggatctgccg ggccagcagg cgtggcgcga gcggcctggg agtcgtgtct gcctgcgcgc
     1261 tgtagccaat gacatccacg ccgcgatcgg ccgcgcgcgc ggcgacgcga caaccggccg
     1321 gatcgtcgag attgatcacc gcgtggcgga gtccgggcca ctcgaacagc attgccttgg
     1381 cggcttcgta gcgctccatc gttccatgaa cgtcgaggtg gtcgcgcgtc aggttggtga
     1441 agagcgccac gtcgaagtgc atgccgtcga ctcggcgctg ctcgagtccg atggacgaga
     1501 cttccattgc cagggcctgt gcgccggcgt cgagcaggtg ccgcagttca cgctgaagcg
     1561 acaccgggtc gggcgtggtc agctcactgg catgcagaga ccccagaaag ccgataccga
     1621 ccgtgccgac gacggcacag cgacgtcctt catgcgtcag cagttgagcg atccactggc
     1681 tgcacgaggt cttgccgtta gtcccggtca caccgatcga tagcagctgc tcactgggcc
     1741 gctcgtaata cgccgccgcc agtgcgccga ggctgtcgcg cagaccattc acaggcagca
     1801 ccgggatcac ttgagcggca ccgtcccagc cgtcggcatc gaccagtgcc gccgccgcgc
     1861 cgcggtcgat ggcatcggcg aggaactgtc gaccgtcgct cctgcctccc ggcacggcga
     1921 ggaagatatc gccggcacgc acggctcgac tgtcgagtcg caggtcgcgc tcacccgagg
     1981 caagcggcag ttcatgcaga aacgcgagcg catcccgcac gcagggcgac atggcggtcg
     2041 atggggtggc agcaaccatc gtcatcgtgc ctgccgcagc gcgagcaact gcttcggtac
     2101 ctcgagcggg ccatccgggg ccacctgcag cgtgcgcagc gatgcgccgg cgatctgcgc
     2161 gaacaccggc gccgccacct ggccaccgta gtacgcgcca ccgctcggtt catcgaccat
     2221 caccgcgatc acgatgcgcg gatccgagac cggcgcgaag ccgacaaagg acgcgacata
     2281 ctccttcacg tactgcccgt ttttcagctt gtgcgcggtg ccggtcttgc ccgccacgcg
     2341 gtagccggca atgcgagcgg ccggggccgt tccgtcgtcg gccaccgcca tctcgagcat
     2401 ctttcgcatc gctcgcgccg tctccacgga cagcacgcgc accgcgatgg gcgaaacctc
     2461 ggacttggtc agtgttagcg ggaccagttc accatcgcgc gccagcgccg cataggcgcg
     2521 agccagttgc accagggtca cggacacgcc gtgtccatac gagatcgttg cctgttcgat
     2581 cggacgccag gtctttgccg ggcgcaggcg tccggccacc gcgccgggaa atcccatgcg
     2641 gggtgcctgc ccgaaaccga gtgccgtgta gctgtcccac agcgactggg ctggcaggtc
     2701 gagcgcaatc ttggcggtgc cgacgttgct cgacttcgct acgacttctt cgacagtcag
     2761 caggccgtgc gcgtgtgcgt cgccgatcgt gcgatcgccg atcgtcatgc ggcccggcgc
     2821 ggtctgtatg cgtgtatcgg ggcggacctt gccgagctcg agcgccgtcg ccactgcgaa
     2881 cggtttcatc gtcgaacccg gctcgaaggt atcggtgatc acgcgattgc gaatcgcgtc
     2941 gggcgcgaga cggccacgcg cattcgggtc gtagctcggc cagttcgcga gcgcgagcac
     3001 ttcaccgctg gcgacatcga gaacgattac tgccccggcc ttggcctggt gcttgtcgat
     3061 cgcgcccttg agcgccgaga acgcgatgta ctggacccgg ttgtcgatgg acaacgcgag
     3121 gtcacggccg tccaccggct cgcgcagcca gtcgtcctcg accacccggc cgaggcgatc
     3181 cttgatcaca cggcggctgc cggcgcgccc ggccaacgtg gactcgtgcg cgagttcgat
     3241 gccctcctgt ccgcggtcct cgacattcgt gaagccaacg agatgcgcgc tggtcggccc
     3301 ttccgggtaa tgtcgcttga actcgcggct cgagtgaacg ccggaaatct tcagcgccgc
     3361 aatgcggtcg gccacgtcga catcgacctg ccgccgcaga tacacgaagc tgcgatcctc
     3421 attctccaga cgccgcttca cttcggcggg gttcattccc agcagccgcg cgagatcggc
     3481 gacctgtgcc ggggtcgcca cgacatcgtc gggaatcgcc cagacagccc gcgccggcac
     3541 cgacgacgcc agcacgatgc cattgcggtc ggtcacccgg ccgcgcgttg cgggaatctc
     3601 gagcgtgcgc gcataacgcg cttcgccctg gcgctgaaga aaatccgtcg tcaccccgcc
     3661 catcaggtag aacgcccggc cacccaaggt gacgaagccg acgaagagca tgaacagcaa
     3721 gagcttcgag cgccaggcgg gcagcgccac agtcagcagc ggattcgtat tgaacgccac
     3781 gcgctcgcgg cgcgaggcgc cggactcgga ccgccgcgca gggcgcgcgt tggagctgcg
     3841 cgagttgcct ttcacttgcg cgctcccggt gccaccggtg tggtggcgct cgcctgcggc
     3901 aggttgagga acacggtgcg gctcgcctcg gcggccttga atcccagccg cagcgccgcg
     3961 cgttcgaccg cggccggctg cgcgagtcgc gccagttcaa tcgttaggcg ctcgtaattg
     4021 acatcgagct gtttggcatc ctgctgcgca cgctcgagat cgacgaagag tgaacgcgta
     4081 cggtgctgcg cggtaaccag tccgagcgac gaagcgaaca gcagcacgac tgcgaagtac
     4141 accagtacgt ggcggatcat cgggccgctc cgggccaggc ggcggccgtc cgttcggcga
     4201 cgcgcatcac tgccgagcgc gcccgtggat tgacggcgac ctctgcgtca tcggcccgca
     4261 cgcgtgtgat cgcggcgagc cgtggcgcag gcagctcggc ggcgcgcagc ggcaaagcac
     4321 gagtacccgg cgcagcacgg tcaggatgtg cgtgcgcatc gatgaagcgc ttgacgatgc
     4381 gatcttcgag cgagtgaaag ctgataacga cgagccgtcc tccggtcgcc agcacctgca
     4441 ccgattgttc aagggctagc gccaattcct caagctcttg attgatgtga atccgtacag
     4501 cctgaaatgt ccgcgtggcc ggatgctgtg tcccgtccgt gcggcttttg cgggggatcg
     4561 cgcctgccac gagcgcggca aggtcgccgg tggttgatac cggccgccca gcctcgcggc
     4621 gagccacaac cgcctttgca atcgatgaag caaaccgttc ttccccatag ctgcggatca
     4681 ctcctgtgag ttcatcgatc gatgcccgcg cgagccactg cgccgccgtc tcgccgcgcg
     4741 tcgtgtccat tcgcatgtcg agtggcccgt cggagcgcca tgagaaacct cgttccgctg
     4801 tgtcgatctg tggtgacgac acgccgatgt cgagcagcac accctgggcg ctcgtgaccc
     4861 caagtgccgc gagtgtctcg cccagatgtg aaaaggcggt gtgctcgatg tgaagtcgcg
     4921 gatcgcggat gtcggcagcc gctgccactg ccgccggatc gcgatcgaac gcaatcagac
     4981 gccccgtcgg agccagccgc tccaggatca gacgggcatg cccgccacgt ccgaaagtcg
     5041 cgtcgacata cgtggcgcgc gggtcggtca ccagcagcgc gaccgccggc acggcgagaa
     5101 cggcttcgtg cccgcgggca tccgttgatg ggaccggcgt cattcaccgt cagaacgtga
     5161 aatccgcggc ggagtcggga aggccattgg ccagcgactg gcgttcgtgt tcgcccagca
     5221 gcgctacgtc ccacaactcg aagtgcgcgc ccatgccgag cagcatcacc tcgcggtcga
     5281 gcccggcggc gctgcgcagt tctgcagtga cgagaatgcg accggcgctg tcaagttcaa
     5341 tgtcgaccgc gctgccaagc agcagtcgct gcagcgcccg cgcggcatag ggaagctggg
     5401 cgatctgctg gcggcgaact tcccaggtgg gtcgcggata gatgagcacg cacccatcgg
     5461 gatggcgggt cagcgtcagg cgcccttcgc aactggcggc aagcgcgtcc cgatggcgcg
     5521 caggcatcga caggcggccc ttgccgtcaa gcgtcaggtg agaggcgcct tggaacacgt
     5581 cgattgagca tgggccgctt ctggcttcgc ttttaggcga agcaagcact aactagccca
     5641 ctgtctccca ctatttcaca cggggcgcca ctgtatcgca ccgctttcca gcaggtcaag
     5701 tgaaatgcag ggattttttc aatgacaaca aggacttagc gcccgttttt aacgcggatt
     5761 cacgatgaaa tactacgcca attgaatggc ttagcgctgc atctgcaagt gaaatctcag
     5821 gaaagtcagc gcacggcatc ggccgcgcca cctgaaaatg gcgtcaccgg tcgcgcgatg
     5881 cgcagcgaca ggcccagctg catgagtccg tgcattctct gataggcgga agctattgag
     5941 tcaacccgcc gatcatgggt tcggacgtcc ggcgttaaca ggtaatagag aaaatcgatc
     6001 ggccccgaga gccggggccg accaggaaaa aagcaaagtc cgcctgtaag ccggattctg
     6061 ttcggcgtcc ttgcgaacgc cgtgacgatc attcatctgg accggcgatc actcgccggc
     6121 tcgagccacc tacccgcggc ctctccgagc aggatcgacg gccgcctatt tggtgttgct
     6181 ccgcgtagag attgcccgtt tcacccgaac tgaatcggct cgtctctgtt gctctaatcc
     6241 tcacctcccg gtgggcaggc gttacctgct acgccgctct gcggagtccg gacgttcctc
     6301 ggcttcggat cgaagacgcg gtcgcccgga gaactttgca gagcgaattt tacgcgcttg
     6361 ccggtgccgc ggcacggttc tctcccgatg tcacgataag cgcgcgaatt ccttcgcttc
     6421 ccgttggcgc cgtcacgggt gggcggctta ccgtgctggc gtcgacccgc ccgggtccaa
     6481 atccgccaga atgcgagccg ttgt
//
LOCUS       DATIRT010000497         2195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1170752, whole genome shotgun sequence.
ACCESSION   DATIRT010000497 DATIRT010000000
VERSION     DATIRT010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170752"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="VLN25_11460"
     CDS             complement(<1..791)
                     /locus_tag="VLN25_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HSQ11208.1"
                     /translation="MTYLAKPKLRHPALPINRLGYTRRDYEGKISTLCAGCGHDSISA
                     AIIQACWELEIEPHRVAKLSGIGCSSKTPDYFLGNSHGFNSVHGRMPSVLTGANLANR
                     DLIYLGVSGDGDSASIGLGQFAHMVRRGVNMTYIVENNGVYGLTKGQFSATADRGSKS
                     KRGAVNRDAAIDLVSLAIQLGITYVARSFSGDKDQLVPLIKGALHHSGTALIDVLSPC
                     VAFNNHAGSTKSYDYVRAHNDRTESGGFARGTSGYNIPRCSHSLP"
     gene            complement(799..>2195)
                     /locus_tag="VLN25_11465"
     CDS             complement(799..>2195)
                     /locus_tag="VLN25_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HSQ11209.1"
                     /translation="MLGIDPEVIAQLLGEQYKGKDKLIKPNLDAFMTGLHYGREHLED
                     VVGLRIEASDKVGDRIFVEGNAAAALGCVYGGATVCAWYPITPSSSLAEAFQKYCSRY
                     RVDRQTGRNKFAIVQAEDELASIGMVIGAAWNGARAFTATSGPGISLMTEFIGLAYFA
                     EVPAVIIDVQRGGPSTGMPTRTQQSDLLTCAYASHGDTKHVLLFPEDPHECFEFAAAS
                     LDLAERLQTPIFLMTDLDIGMNHRLCAPFKWDDARRYDRGKVMTPEELDSGRDFGRYL
                     DVDGDGIPFRTLPGAHPTRGAYFTRGSTRDAYARYSEAGPDYVYNMERLLRKFDSAKA
                     LVPQPIVRKPAQKAAAAVIYYGSTAPAMDEALGLLEQQGVHVSRMRLRAFPFPDDVER
                     FIDEHETVFIVEQNRDAQMRTLLVNELEIDPARLMPILHFDGTPITARFIAQAIGDRI
                     KAHNVRPLKSGKAA"
BASE COUNT          387 a          670 c          732 g          406 t
ORIGIN      
        1 caaggcaaac tatgcgaaca tctgggaata ttatacccac ttgtcccgcg cgcgaaccca
       61 ccagattcag ttctatcgtt atgcgcgcgc acgtagtcat agctcttggt gctgccggca
      121 tgattgttga atgcgacgca agggctgagg acatcaatca gcgcggtgcc gctgtggtga
      181 agcgcgccct tgatcagggg cacgagctgg tccttgtcac cggagaagct gcgcgcgaca
      241 taggtgatgc cgagctggat cgcgagcgaa accaggtcga tcgcagcgtc gcgattgacg
      301 gcgccacgct tggacttcga gccgcgatcg gccgtggctg aaaactgtcc cttggtcaga
      361 ccgtatacgc cgttgttctc gacgatatag gtcatgttga cgccgcgccg gaccatgtgg
      421 gcgaactggc cgaggccgat ggaggccgag tcgccgtcac ccgacacgcc aaggtagatc
      481 aggtcgcggt tcgccaggtt tgcgccggtg agcaccgatg gcatgcggcc atggaccgag
      541 ttgaatccgt gcgagttgcc gaggaaatag tccggggtct tggacgagca gccgatgccc
      601 gagagcttcg cgacacggtg cggctcgatc tcgagttccc agcacgcctg gatgatcgcc
      661 gcggagatcg aatcgtggcc gcagccggcg cacagcgtcg agatcttgcc ctcgtagtca
      721 cgacgcgtat agccgaggcg attgatcggc agcgcagggt gacgcagctt cggcttggcg
      781 agataggtca tgtcggactc atgcagcctt gccgctcttc aaggggcgga cattgtgtgc
      841 cttgatgcga tcgccgatcg cctgcgcgat gaaacgcgcg gtgatcggcg tgccgtcgaa
      901 atgcagtatc ggcatcagtc gcgccgggtc gatctcgagt tcgttgacca gcagggtccg
      961 catctgcgcg tcccggttct gctcgacgat gaacacggtc tcgtgctcgt cgatgaagcg
     1021 ctcgacgtca tcgggaaagg ggaacgcgcg caggcgcatc cgcgacacgt gcacgccctg
     1081 ctgctcgagc aggccgagcg cctcgtccat cgccggcgcg gttgaaccgt agtaaatgac
     1141 agcggcggcg gccttctgtg cgggcttccg cacgatcggt tgcggcacca gtgccttcgc
     1201 gctgtcgaac ttccgcagca gccgctccat gttgtagacg taatcgggac ccgcttccga
     1261 gtagcgggca taggcgtcgc gcgtgctgcc gcgcgtgaag taggcgcccc gcgtcggatg
     1321 ggcgccgggc agggtgcgga acgggatccc gtcgccgtcg acgtccaggt aacgcccgaa
     1381 gtcccgcccg gagtcgagtt cctcgggcgt catgaccttg ccgcggtcgt agcggcgagc
     1441 gtcgtcccac ttgaatggtg cgcacagccg atggttcatg ccgatgtcga gatcggtcat
     1501 caggaagatc ggcgtctgca ggcgctcggc gagatccagc gaagcggctg cgaactcgaa
     1561 gcactcgtgc ggatcttccg gaaacagcag cacgtgcttg gtgtcgccgt gcgaggcata
     1621 ggcacaggtc agcaggtcgg actgctgtgt acgtgtcggc atgccggtcg acggaccgcc
     1681 acgctgcaca tcgatgatga ccgccgggac ctccgcgaaa tacgcgagcc cgatgaactc
     1741 ggtcatcagc gagattccgg ggcccgatgt cgccgtgaat gcacgggccc cgttccatgc
     1801 ggcgccgatc accatgccga tcgatgcgag ttcatcctcg gcctgcacga tcgcgaactt
     1861 gttgcggccg gtctggcgat cgacgcggta tcggctgcag tacttctgga aggcctcggc
     1921 cagtgacgac gatggcgtta tcgggtacca cgcgcagacc gtggcaccgc cgtagacgca
     1981 accgagggcc gcggcggcat tgccctcgac gaagatgcgg tcgcccacct tgtcgctggc
     2041 ttcgatgcgc agaccaacga catcctccag atgctcgcgc ccgtagtgca ggcccgtcat
     2101 gaacgcatcg aggttgggct tgatgagctt gtccttgccc ttgtactgct cgccgagcag
     2161 ttgcgcgatg acttccggat cgatgccgag catgg
//
LOCUS       DATIRT010000498         1797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_973349, whole genome shotgun sequence.
ACCESSION   DATIRT010000498 DATIRT010000000
VERSION     DATIRT010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973349"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VLN25_11470"
     CDS             complement(<1..391)
                     /locus_tag="VLN25_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HSQ11210.1"
                     /translation="MKTTTSTTEGPQEHVLTASGEFDVHDEPIDLAVYRFEDWISFAF
                     FWLLAATVFLQFFTRYALNDSAGWTEEIARYLLICTVFIGAAVSVRKNNHIHVDFFDR
                     ILPKRLMRVMSTLVDAARIAFFAYATWL"
     gene            complement(443..1153)
                     /locus_tag="VLN25_11475"
     CDS             complement(443..1153)
                     /locus_tag="VLN25_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HSQ11211.1"
                     /translation="MPIKAVEPQRLYRRIAEQIARLIRRGEYKPGERLPPERDLARQL
                     EVSRPSVREALIALEVEGYVEVRVGSGVYVTQRRPAMRSVDPLPGDSGPFELISARRL
                     LESECAALAARQASPAQMRRMRAALTAMERDRRRAVMPLDNDRRFHLEIAEGSGNSAM
                     ALVVRTLWDQRTGPLFLRLERHFDTPDLWAAAIAEHNAVMTAIEAHDSSGARAAMRRH
                     MDAAARRFQRSWSAGAPN"
     gene            1323..>1797
                     /locus_tag="VLN25_11480"
     CDS             1323..>1797
                     /locus_tag="VLN25_11480"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSQ11212.1"
                     /translation="MAGRLQGKSALVTAAGQGMGHAAVVAFAREGARVTATDLKPELL
                     ADVPKGVAARGLDVLDDDAVQDFVGGLGAVDILFNCAGTVHQGTILDCSIADWDFSFD
                     LNVRSMFVLTRAMLPKMIAAGGGVILNMASVLGAHKAAPNRLAYAASKAAVVGFTR"
BASE COUNT          307 a          587 c          606 g          297 t
ORIGIN      
        1 tcaaccaggt cgcataggcg aagaatgcga tacgcgcggc gtcgacaagt gtcgacatca
       61 cgcgcatcag ccgcttcggc aggatgcgat cgaagaagtc gacgtggatg tgattgttct
      121 tgcgcacgct gacggctgca ccgatgaaaa cggtgcagat cagcaggtag cgcgcgatct
      181 cctcggtcca gccggccgag tcgttcaggg cgtagcgcgt gaagaactgc agaaagacgg
      241 tcgcggcgag caaccagaaa aaggcgaacg agatccagtc ctcgaagcga taaacggcga
      301 ggtcgatcgg ttcgtcatgg acatcgaact caccactcgc ggtgagcacg tgttcctgtg
      361 gtccttcggt cgtcgacgtc gtcgtcttca tgcattctca atgaggtgcg gacgaaaccg
      421 cccacgggtc tcgtctcgtg tatcagttcg gcgcgccggc actccagcta cgctggaacc
      481 gcctcgccgc ggcatccatg tggcgccgca tcgctgccct cgcgccgctg gaatcgtgag
      541 cttcgatggc ggtcatcacc gcgttgtgtt ccgcgatcgc ggcggcccac aggtccggcg
      601 tatcgaaatg acgttcgagc cgcaggaaga gcggtcccgt gcgctggtcc caaagcgtgc
      661 gcaccaccag cgccatggcg ctgttgccgc tgccctcggc gatctcgagg tgaaagcggc
      721 ggtcgttgtc gagcggcatg acggcgcgcc gtcggtcacg ctccattgcc gtcagggcgg
      781 ccctcatgcg tcgcatctgg gcagggctcg cctggcgcgc ggcgagcgcg gcacactcgg
      841 actcaagcag gcgacgtgcc gagatcagtt caaacggtcc ggaatccccc ggcaggggat
      901 cgacactgcg catcgcagga cgccgctgag tgacgtacac gcccgagccg acacgcacct
      961 cgacgtagcc ctcgacctcg agcgcgatca gcgcctcgcg cacggacggg cggcttacct
     1021 cgagctggcg cgccaggtcg cgctcgggcg gcagacgctc gccaggcttg tattccccgc
     1081 ggcggatcag ccgggcgatc tgttcggcga tgcggcggta cagccgctgc ggttcgacgg
     1141 ccttgatcgg catgacgagg gttcactctc ccgtgcagaa ttgtcgtcgg caggctggac
     1201 agctcagagt tgcgccgctt acactgccgc gcactggtca agtggtcaga ccaattgcgc
     1261 ggctagcttc acacgcacac cccggggtgt caagccgcag cgggaagcat cggaggatgg
     1321 cgatggcggg gcgtttgcaa ggcaagtcgg cgctggtgac agccgccggc cagggcatgg
     1381 ggcatgccgc cgtcgtggcc ttcgcccgcg aaggcgcgcg cgtcaccgcc accgacctga
     1441 agcccgaact gctggcggat gtgccgaaag gcgttgcagc gcgcggcctc gatgtcctcg
     1501 acgacgacgc cgtgcaggac ttcgtcggcg ggctcggcgc cgtcgacatc ctcttcaact
     1561 gcgccggcac cgtgcaccag ggcacgatcc tcgactgttc gatcgccgac tgggatttca
     1621 gcttcgatct caacgtgcgc agcatgttcg tgctgaccag ggccatgctg cccaagatga
     1681 tcgccgcggg cggcggcgtg atcctcaaca tggcgtcggt gctgggcgcg cacaaggcgg
     1741 cgcccaaccg cctcgcctac gcagcgagca aggccgcggt ggtcggcttt acgcgcg
//
LOCUS       DATIRT010000499         2219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1072834, whole genome shotgun sequence.
ACCESSION   DATIRT010000499 DATIRT010000000
VERSION     DATIRT010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072834"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /locus_tag="VLN25_11485"
     CDS             complement(<1..1090)
                     /locus_tag="VLN25_11485"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSQ11213.1"
                     /translation="MHDDVSKSRRAFAQGLAATPAALAMRPADGRSPGNAETKVLRYA
                     FRVAETGFDPAQVNDLYSSTVNANIFDAPLTYDFLARPARIVPNTAVAMPELSADFST
                     LTVQIRPGIVFQDHPAFKGTPRELVASDYVYSIKRFFDPRFKSPRLYLLENARLFGMA
                     DLRAAALKGARFDYDREVDGVRALDRYTLQLRLGAPNPRFFQYLADNAFMGAVAREVA
                     ESYDEKAIMGHPVGTGPFRLVDWRRSSRIVLERNPTYRRDVYRAQPPTGDESGAALAA
                     RLAGRTLPMIDRIEVYIVEENQPRWLAFLNGDHDLVDEIPVDLANLIVPNGVLAPNLS
                     KRGIRMDRAARPSVDMALFNMEHPVVGGH"
     gene            1212..2150
                     /locus_tag="VLN25_11490"
     CDS             1212..2150
                     /locus_tag="VLN25_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSQ11214.1"
                     /translation="MGAYILRRLWQMIPTLVGVILFVFFLFKWFGGDPAEILGGLLAT
                     PEQIEAIRTQLGLNEPWYVQLWIFVKSIFTFNWGNSWATGESVANIFRTRLPATLTVM
                     VPILILETVLGIVAAMAVAYVRGSLTDRSIMIITTVMLSISFLVYIIVGQWLFGFYLG
                     WFPVQGWSDSFWTNLTHYAVLPVLLATFVSLAPNTRLYRSFFLDEINQDYVRTARAKG
                     VSENRALFVHVLRNAMIPVLTNVAVQLPGIFLGSFLIETFFSIPGLGRETLLAVNRSD
                     YPVIQAVTVYLAALTMVINLGADILYKLVDPRVVLK"
BASE COUNT          388 a          670 c          720 g          441 t
ORIGIN      
        1 catggccgcc aacgaccgga tgctccatgt tgaacagcgc catatcgaca ctgggccttg
       61 cagcgcggtc catccggatt ccgcgttttg agagattcgg cgcaaggacg ccgttcggaa
      121 cgatcagatt ggcgaggtcg accgggatct cgtcaacgag gtcatggtcg ccgttcagga
      181 acgcgagcca gcgcggctgg ttctcctcga cgatatagac ctcgatccgg tcgatcatcg
      241 gcaacgtgcg acccgccaaa cgcgccgcga gtgcggcacc actttcgtcg cctgtcggtg
      301 gctgggcgcg atagacatca cggcggtagg tcggattgcg ttcgagcacg atgcgtgagg
      361 aacggcgcca gtcgaccagc cggaacggtc cggtgccgac cgggtgtccc atgatcgctt
      421 tttcgtcgta gctctcggcg acctcgcgcg ctacggcccc catgaatgcg ttgtcggcga
      481 ggtattgaaa gaaccgcggg ttgggagcac cgagtcgcag ctgcagcgtg taccggtcaa
      541 gcgcgcgcac gccgtcgacc tcgcggtcat agtcgaagcg cgcgccctta agcgcggccg
      601 cacgcagatc ggccatgccg aacaggcgag cgttctcgag caggtacagg cgcgggctct
      661 tgaagcgagg atcgaagaag cgcttgatgg aatagacgta gtcggacgca accagctcgc
      721 gcggagtgcc cttgaacgcc gggtggtcct ggaagacgat gcctggacga atctgcaccg
      781 tcagtgtcga gaagtctgcg gacaactcgg gcatggccac tgccgtattc ggcacgatcc
      841 tggccggtct cgcgaggaag tcgtaggtca gcggtgcgtc gaagatgttg gcgttgacgg
      901 tgctcgagta aagatcattg acctgggccg gatcgaagcc ggtttcggcc acgcgaaagg
      961 cgtagcgcaa caccttggtt tcagcattgc ccggcgaccg tccgtcggcc ggacgcatcg
     1021 ccagtgccgc tggcgtggca gccaggccct gggcaaaggc gcgtcggctt ttcgagacgt
     1081 cgtcgtgcat ccgcgtacag tggctgaaac cgccttattt gaataccggg cgggcatcgg
     1141 cataatccgc gctttcgcgc gccgggtcac tacgaggcgg gcgcgagcaa cgacagaaag
     1201 agtcggcccg cgtgggcgcc tatatcttac gcagactgtg gcagatgatc ccgaccctgg
     1261 tcggggtcat cctctttgtc ttctttctgt tcaaatggtt cggcggcgac cccgcggaga
     1321 tcctcggcgg gctgcttgcg acaccggagc agatcgaggc gatccgtacc cagctgggtc
     1381 tgaacgaacc ctggtatgtc cagttatgga tattcgtgaa gtcgatcttc acgttcaact
     1441 ggggcaacag ctgggcgacc ggcgaatcgg tcgcgaatat cttccgcacg cgactgccgg
     1501 cgacgctgac tgtgatggtg ccgatcctga tcctcgagac cgtcctcggg atcgtcgccg
     1561 ccatggcggt ggcctatgtc cgcggttcgc tcacggaccg gtcgatcatg atcatcacga
     1621 cggtgatgtt gtcgatctcg tttctggtct acatcatcgt cgggcagtgg ctgttcggtt
     1681 tctacctcgg ttggttcccg gtgcagggct ggagtgacag cttctggacc aacctgactc
     1741 actatgccgt gctgccggtg ctgctggcta ccttcgtgtc tctggcaccg aacacgcggc
     1801 tgtaccgcag cttcttcctc gacgagatca accaggatta cgtgcgcact gcgcgcgcca
     1861 agggcgtcag cgagaaccgg gcgctgttcg tgcacgtgct gcgtaacgcg atgatcccgg
     1921 tgctgaccaa cgtcgcggtg caactgccgg gcatcttcct gggctctttc ctgatcgaga
     1981 ccttcttttc gatacccggc ctggggcgcg agacgctgct ggccgtcaac cgcagcgact
     2041 atccggtgat ccaggccgtg accgtttacc tcgcggcgct gacgatggtg atcaacctcg
     2101 gggccgacat cctctacaag ttggtcgacc cgcgggtcgt gctcaaatga gcgtgctgaa
     2161 gtgaggatgc gatgacgact gttgtcgagg cctatcaggc gccgtccacc gagcgatcc
//
LOCUS       DATIRT010000500         2553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1072867, whole genome shotgun sequence.
ACCESSION   DATIRT010000500 DATIRT010000000
VERSION     DATIRT010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072867"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(238..540)
                     /locus_tag="VLN25_11495"
     CDS             complement(238..540)
                     /locus_tag="VLN25_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HSQ11215.1"
                     /translation="MEQVTKERLMTDVNTVLVDAEELLRQAAQASGEQAADLRRRAQS
                     AIANAKVRIGEVEHRVVEQAKSTARATDGWVHEHPWTAIGVAAGIGVLVGLVINRR"
     gene            complement(630..2054)
                     /gene="dacB"
                     /locus_tag="VLN25_11500"
     CDS             complement(630..2054)
                     /gene="dacB"
                     /locus_tag="VLN25_11500"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HSQ11216.1"
                     /translation="MLWLLRCVAILGLLLAAVQPAGSQATRAAQDLPETVATALRVAN
                     VPTSAVGISVVPLTGTGLTLSVNEGALMNPASTMKLVTTLAGLEILGPQFTWRTEALT
                     IAPLTAGTLEGDLYLRGSGDPRFVVEHLWLLIQRLRGLGLREIRGDLLLDRGSFEAAA
                     HDPGAFDGENLRPYNAGADALLLNYKTVTFHFGPDPAAKQVRVYAVPAMAGMALPATV
                     RAAEGPCNDWRARLGGDFTDPMRPQFRGAFPLSCGDRAWHVSLLNPTSYLDAVFRALW
                     TDSGGIWRGRAREGVAPADARRLAQHESESLAAVIRDINKFSNNVMARQLFLTIGGEV
                     AKQPANADRAQRAVGDWLVGKGLERRDFVLENGAGLSRVERLTPTGLSRLLASAFASP
                     LMPEFASSLPLVGVDGTMRKRTGAAGSAHIKTGLLADTRAIAGYVLAASGRRYAVVAF
                     VNHPNANVSQGALDELLNWVYVNG"
     gene            2352..>2553
                     /locus_tag="VLN25_11505"
     CDS             2352..>2553
                     /locus_tag="VLN25_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11217.1"
                     /translation="MPPLVIPEPPAGGFAPSGPTAAPAAPSDSQAVRFLAQSTFGATS
                     AEIDRLKRLGYNDWIEQQFQIPR"
BASE COUNT          432 a          864 c          822 g          435 t
ORIGIN      
        1 gtcatcaggg cggcgagcag cagcacggca gcaacgacgc cgagcgcggc ccagcgcagc
       61 gctcggtcgc gtgtttcttc aagctcgagc gccgccagtt caccgcgtgt cgccagcagg
      121 ctgatcgcgt tggagagcat cgcccgcatc gatgcgatca agccaggcgg ggactccgtg
      181 tgatccgaca tcgcggctgc caccgtgggc gcgcctcgca ctgattttga gggatgatca
      241 gcgacggttg atcaccaagc cgaccagcac gccaatgccg gcggcgacac cgatcgctgt
      301 ccacggatgt tcgtgcaccc agccatcggt ggccctcgct gttgacttgg cctgctcgac
      361 cacccgatgc tcgacttcgc cgatccggac cttcgcgttg gcgatcgccg actgagcgcg
      421 gcgtcgcaga tcagcggcct gctcgccact ggcctgggca gcctggcgca gcaattcctc
      481 tgcgtcgacc agcacggtgt tgacatccgt catcaagcgc tctttcgtga cctgttccat
      541 ggtttctcct tcaagtgaac caactgcatt tccagcttag acagtcatca tgcggtcggc
      601 atgatgcaga tcgaatcccg caaattccct tagccgttga cgtagaccca gttcagcagt
      661 tcatccagcg cgccctgcga gacgttggcg ttcgggtgat tgacgaacgc gaccacagca
      721 tagcgtcgac cgctcgcggc cagcacataa cccgcaatgg cacgagtgtc ggccagcagg
      781 ccggttttga tatgggcgga cccggctgcg ccggtccgtt tgcgcatcgt gccgtcgacg
      841 cccacgagcg gcagcgacga cgcgaactcg ggcatcaacg ggctggcgaa ggcagaggca
      901 agcaggcgcg aaaggccggt tggcgtcagg cgttcgacgc gcgacagccc ggccccgttt
      961 tcgagcacga agtcccgccg ttcgagcccc ttgcccacca gccagtcgcc cacagcgcgc
     1021 tgcgcacgat cggcattcgc cggctgcttc gcgacttcgc cgccgatcgt caggaacagc
     1081 tgtcgcgcca tcacgttgtt gctgaacttg ttgatgtcgc gaatgactgc ggcgagggat
     1141 tcggattcat gctgcgcaag ccgccgcgca tccgccgggg cgaccccctc gcgcgcgcga
     1201 ccgcgccaga tcccaccgct gtcggtccac agcgcgcgga acacggcgtc gagatacgag
     1261 gtggggttga gcagactgac atgccaggcg cgatcgccgc acgacaacgg aaatgcgccg
     1321 cggaactgcg gtcgcatcgg atcggtgaag tcaccgccca gtcgggcacg ccagtcgttg
     1381 cagggcccct cggccgcccg taccgttgca ggcagcgcca ttcccgccat cgcaggtacg
     1441 gcgtacacac ggacctgctt cgccgcgggg tccggcccga agtgaaacgt gacggtcttg
     1501 tagttgagca gcagggcgtc cgctcccgcg ttgtagggtc gcaggttttc gccgtcgaaa
     1561 gccccgggat cgtgcgccgc cgcctcgaac gagccccggt cgagcagcag gtcgccgcga
     1621 atctcgcgca gtccgagtcc ccgcagccgc tggatcagca gccacaggtg ttcgacgaca
     1681 aagcgcggat cgccgctgcc gcgcagatag agatcgcctt cgagcgtgcc cgcggtcagc
     1741 ggcgcgatgg tcagggcctc ggtccgccag gtgaactggg ggccgagaat ctcgagaccg
     1801 gccagcgtgg tgacgagttt catcgtcgat gccgggttca tcagcgcgcc ttcgttgacc
     1861 gacagggtca acccggtgcc ggtcagcggc acgacgctga tgcccacagc gctggtcggt
     1921 acattcgcga ccctcagcgc cgtggcaacc gtctccggta aatcctgggc ggcgcgggtc
     1981 gcttgcgatc cggccggttg aaccgctgcc agcaatagac ccaggatggc aacgcaccgc
     2041 agtaaccaca gcacggacaa ggatttcctc tctggcgaag cgttcaatgg gtcggcgcag
     2101 ttgcaggagc tagttcattc gtccgataga cccacccagg cacggtcgct agcctcccac
     2161 tggggtgaac cgcgatgtcg cggtgtctca tggcgcgcct gatcgccttc tgttccggaa
     2221 acccgatgaa cttgttgccc gctttcaagg ttcgaagctt actggctgct gctgcagctg
     2281 cactgacagt gtcggcatgc ggtggcggcg gcggagatac tggggcatcc gcatcggcac
     2341 ctgcgccggc tgtgccaccg ctcgtcattc cggagcctcc cgcggggggc ttcgcgccgt
     2401 ccggcccgac cgccgcgccc gcggcaccgt cggattcgca ggcggtgcgc ttcctggcgc
     2461 agtccacctt cggcgcaacg agtgcagaga tcgaccggct gaagcggctt ggctacaacg
     2521 actggatcga acagcagttc cagatcccgc ggt
//
LOCUS       DATIRT010000501         2175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1072905, whole genome shotgun sequence.
ACCESSION   DATIRT010000501 DATIRT010000000
VERSION     DATIRT010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072905"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /gene="msrB"
                     /locus_tag="VLN25_11510"
     CDS             <1..414
                     /gene="msrB"
                     /locus_tag="VLN25_11510"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HSQ11218.1"
                     /translation="MQKVTKTDAEWQAQLSPMEYAVTRGKGTERAFTGRYWDHHELGI
                     YRCVGFGTALFASDTKFDSGCGWPSYFKALNPDHVREERDTSHGMDRTEILCNICDSH
                     LGHVFPDGPPPTGLRYCINSASLTFEPAQQESGSR"
     gene            411..1037
                     /locus_tag="VLN25_11515"
     CDS             411..1037
                     /locus_tag="VLN25_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HSQ11219.1"
                     /translation="MKLLFDLLPVILFFVTFKVAGSYPQQSLALAGSTLGWMVSDGAV
                     PADQAPILLATAVAIIASVVQVGSVLVRGRRVDPMLWVSLGVIVVFGGATIWFHDETF
                     IKWKPSILYWLFGASLLAGHLVWKKNLLHTLLGTQLEVPPLVWDRLLWAWIVFFALMG
                     IANLAVAYSVPTDTWVNFKLFGLFGLTLVFTLGIGIYLSRHMKEAPDA"
     gene            1030..1341
                     /locus_tag="VLN25_11520"
     CDS             1030..1341
                     /locus_tag="VLN25_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HSQ11220.1"
                     /translation="MPEATVNAMRARLAVLAPIELAIEDESALHAGHPGAASGGGHYR
                     MTLVSAAFAGQPRVKRHRLVYDALADLMQREIHALAMTLLAPDETQPGPAPSLSQFSG
                     H"
     gene            1341..2114
                     /locus_tag="VLN25_11525"
     CDS             1341..2114
                     /locus_tag="VLN25_11525"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSQ11221.1"
                     /translation="MLMQRPALFAALALASGIALAQNVAVVNNKPIPKAREEAWVKEL
                     TKQGQKDSPEIRAMVKERLIQNEVLLQEAGKRGIAEQADVKFMLDIQRQNTLIQALLR
                     DEMTKNPITDAQVQAEYDKQKGSVGSKEYRARHILVENQADAAAIIEQLKKGTKFEEL
                     AKKSKDTGSAANGGDLDWAAPDAYVKPFSEALLKLKKGETVDVPVQTQFGWHVIRLED
                     VRDTQFPPLEQVAPQIRESLQQQKAQAFVQNLTKSAKIQ"
BASE COUNT          421 a          699 c          675 g          380 t
ORIGIN      
        1 atgcagaaag tcaccaagac cgacgccgag tggcaggcgc agctttcgcc catggagtac
       61 gcggtcacgc gcgggaaagg cacggagcgc gcctttaccg gccgctactg ggatcaccac
      121 gagctcggca tctaccgctg cgtcggcttc ggcacggcgc tgtttgcgtc ggacaccaag
      181 ttcgactccg ggtgcggctg gcccagctac ttcaaggcgc tcaacccgga ccacgtgcgt
      241 gaggaacggg acaccagcca cggcatggac cgcaccgaaa tcctctgcaa catctgcgac
      301 tctcacctcg ggcacgtctt cccggatgga cccccaccca ccggactgag gtactgcatc
      361 aattcggcgt ccctgacgtt cgaacctgca caacaggaaa gcggttcgcg atgaagctgc
      421 tgttcgatct gctgcccgtc attctgtttt ttgtgacgtt caaggtcgcc gggtcctatc
      481 cgcagcagtc gctggcgctc gcgggatcga cgctcggctg gatggtgagc gatggcgccg
      541 tgcccgccga ccaggcgccg atactgctcg cgaccgccgt ggcgatcatc gcctcggtcg
      601 tgcaggtcgg cagcgtgctc gtccgcggcc gtcgggttga tccgatgctc tgggtcagcc
      661 tgggcgtgat cgtcgtgttc ggcggcgcga cgatctggtt ccacgacgag accttcatca
      721 agtggaagcc gagcattctt tattggctgt tcggcgcctc gctgctcgca gggcatctcg
      781 tctggaagaa gaacctgctg cacaccctgc tcggcacgca gctcgaagtc ccgccgctgg
      841 tgtgggaccg tctgctctgg gcgtggatcg tgttcttcgc gctcatgggc attgccaacc
      901 tcgcggtggc ctactccgtg ccgaccgaca cctgggtcaa cttcaagctc ttcggcctgt
      961 tcggactgac gctcgtgttc acgctcggca tcgggatcta tctctcgcgc cacatgaagg
     1021 aagcgcccga tgcctgaggc caccgtcaat gcgatgcgcg cgcgccttgc cgtgcttgca
     1081 ccgatcgaac tggccatcga agacgagagc gcgttgcatg ccggccatcc aggtgccgcc
     1141 agtggcggcg gccactaccg gatgaccctg gtgagtgccg cattcgcggg tcagccgaga
     1201 gtgaagcgac atcgccttgt gtatgatgcg ctcgccgatt tgatgcagcg cgagatccac
     1261 gctctcgcca tgacgctgct ggcgcccgac gagacacaac ccggcccggc gccttccctt
     1321 tcccaatttt ctggacattg atgctcatgc aacgacccgc tttattcgcg gccctggcac
     1381 tggccagcgg aatcgccctc gctcagaacg tcgccgtcgt caacaacaag ccgattccca
     1441 aggcccgcga agaggcctgg gtgaaggaac tgaccaagca ggggcaaaag gactcgcccg
     1501 aaatacgcgc gatggtgaag gaacggctga tccagaacga agtgctgctg caggaagccg
     1561 gcaagcgcgg catcgccgag caggccgacg tcaagttcat gctcgacatc cagcgccaga
     1621 acacactgat ccaggcgctg ctgcgtgacg aaatgacgaa gaacccgatc actgacgccc
     1681 aggttcaggc cgagtatgac aagcagaagg gctcggtcgg cagcaaggaa tatcgcgccc
     1741 gtcacatcct ggtcgagaac caggccgatg ccgcggcgat catcgagcag cttaagaaag
     1801 gcaccaagtt cgaggagctc gcgaagaagt ccaaggacac tggctcggct gccaacggag
     1861 gcgatctcga ctgggctgca cccgatgcgt acgtgaagcc gttttcggag gcgctgctga
     1921 agctcaagaa gggcgagacg gtcgacgtcc cggtgcagac ccagttcggc tggcacgtga
     1981 tccgcctcga agatgtgcgc gacacgcagt tcccgccgct cgaacaggtc gcaccgcaga
     2041 tccgtgaatc actgcaacag cagaaggcgc aggcgttcgt acagaacctg acgaagagcg
     2101 cgaagatcca gtagccgcag gacgttgccg cgtcagcagc ggaccactcg aaggggcgac
     2161 ttaggtcgcc ccttt
//
LOCUS       DATIRT010000502         6939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1073471, whole genome shotgun sequence.
ACCESSION   DATIRT010000502 DATIRT010000000
VERSION     DATIRT010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6939
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073471"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..571)
                     /locus_tag="VLN25_11530"
     CDS             complement(59..571)
                     /locus_tag="VLN25_11530"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11222.1"
                     /translation="MASARPAAHSVGRCAAILLSALATTGTHTAAQTVQRCEAADGKV
                     TYSNAACPDGTKSVRQVNTAPPIAVEEQKAAKDRAKRDVANAKEAEKARDKERETEEA
                     KADRAAAEQKKTQAKERERCDRAKRDLERARTARASLLQQRSATIDEMQKADREISKR
                     ETDAAKACPG"
     gene            complement(571..1026)
                     /locus_tag="VLN25_11535"
     CDS             complement(571..1026)
                     /locus_tag="VLN25_11535"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HSQ11223.1"
                     /translation="MLRSFAQWACAVAFIIPATVAAQPIQRCEGAGGRVTYSNSECPE
                     GTKPIKAVQAAPTPPPEAQREAREKAARDAQAAQQLADQRQAQQATATQQQQERRIAD
                     CAYLRGEIDSIRRLRNALLNRPYYSLDDLDQMDQHAAQLTAQYRRVCSP"
     gene            complement(1110..2186)
                     /locus_tag="VLN25_11540"
     CDS             complement(1110..2186)
                     /locus_tag="VLN25_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11224.1"
                     /translation="MASLLLRTCALALATLATPLALAQQPAEVVVYGPAPKPVVVPGA
                     KPIAAPSFEAFKGDGLYHFQGARVSNIRAAATPGEGNRYALSETSNDSNHTTLNGNID
                     VAHAVLDGERRALGRPNFAIPANDGSVYIVYAGQASLRMAVTLQAYDVSDMPISLFLR
                     TPENYPKQEAAKAGTVRFPKGAVAYLTTARFFDDVLMLPRRESFTGATNTGQFVGNFS
                     KQIPYCLAYEDRQGAKPYAMYFKAAAPGKKGEVSLYPAKTGTLFCDRAVEQVVGQGEW
                     EERTIGGTKAIVLSFPAQVDPLDTGVSNVERESARIAFIEPTKGLPGVRPGKLYQAGA
                     SVYDYQYRFNKVAADAVRQAIGVQ"
     gene            complement(2330..3754)
                     /locus_tag="VLN25_11545"
     CDS             complement(2330..3754)
                     /locus_tag="VLN25_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSQ11225.1"
                     /translation="MSPVDALRTHIDALRTLVGEPHVLTSANDKAPFLLDWRRRYHAQ
                     AQAVVLPGSTAEVARVVAWCAETRTPVVPQGGNTGLVGGASPDESGRAILLSLKRLQR
                     IRSVDTANGTLIAEAGCVLKDVQDAAQMQGQLFPLSLAAEGSATIGGNLSTNAGGVQV
                     LRYGNARELCLGLEVVLPSGQIWHGLRGLRKDNTGYDLRDLFIGAEGTLGVITAACLK
                     TFPLPRARATALLGVPSLPAALQLLAMAQTTCGPTLTAFECFSDFCLTLVERHYPPHR
                     TPFAQRHSQHVLVELSDHEDATHANALLEALAGSAIEAGIGSDAVIAQSVAQARAFWS
                     LRELISEAQALEGKNIKHDIAVPISSIARFVQETDRVLQQRFPGVRMVVFGHLGDGNL
                     HYNVSPPIGMDEPTFLSLQPAVYSTVHDQVHAFGGSISAEHGIGQLKRGENARYKSEV
                     EMGLMRTLKQALDPLGIMNPGKVL"
     gene            complement(3751..4152)
                     /locus_tag="VLN25_11550"
     CDS             complement(3751..4152)
                     /locus_tag="VLN25_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HSQ11226.1"
                     /translation="MKRTAIGPLPQTTPRQRTAWRWAIAAWLGLIALALAWELALAPL
                     RPGGSWLVLKVVPLLLPLPALLRGSAYAMQVTLFIVMIYLFEGAARLFEPTPAAVLAL
                     IELVLAVTFLVAAIVYLRPMKLAAKRRVSAS"
     gene            complement(4149..4739)
                     /gene="wrbA"
                     /locus_tag="VLN25_11555"
     CDS             complement(4149..4739)
                     /gene="wrbA"
                     /locus_tag="VLN25_11555"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HSQ11227.1"
                     /translation="MDILVLFYSRQGATRRLAEAIATGIDSIDGAQARLRTVPPVAAV
                     TAIAAPPVPSEGAPYAELADLEACGGLAVGSPTRFGNMAAPLKHFFDSTSGLWLSGRL
                     AGKPAAVFCSTSSLHGGQETTLLSMMLPLLHHGMLILGIPYTEADLMATRSGGTPYGA
                     SHVARHGAPQVLSDEERRLAHALGVRLARTALRLAE"
     gene            4887..6146
                     /locus_tag="VLN25_11560"
     CDS             4887..6146
                     /locus_tag="VLN25_11560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HSQ11228.1"
                     /translation="MSRLTVALDRFPWLKTAREVALFAYRRGSEVRLQQVAGSLTFTT
                     VLSIVPLFAVALALFSAFPLFAEFRESFQAMVQRTLPAQISATVLRYVSDFAQQATRL
                     TAAGLIFLGLTALAMIMTVDRVLNDIWQVRDRRPFSQRVLIYWALITLGPILIGASLS
                     ASSYLWALSEDAIARMPRGLRGLIDFAPVLLSGLAYAALYVFVPNRTVRWRDALIGGF
                     VAAILAELLKSGFAHYIGRGGVRSIYGAFAALPLFLLWMYLSWYALLFGAAIAATVPR
                     LWTTRFRDELRAGDDFVTAVALIQQLLAARRRGNTAVGVRVLARGARTDPVHAERLLE
                     SLERLRYVHRLSATRRRGESSEWILTCEPASATLRSAFERFALDSSNSLLSLDREGVG
                     NTLLNWIDRDEWINAPLEQSLSDAGAR"
     gene            complement(6207..6839)
                     /locus_tag="VLN25_11565"
     CDS             complement(6207..6839)
                     /locus_tag="VLN25_11565"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HSQ11229.1"
                     /translation="MLHAVNWDTYWLYVLTEIALSLSPGPAVMLVIACGLAHGARRST
                     YASLGILSANAFYFAVSATALGAVLAASREFFTAVKWIGAAYLVYLGVSAIFGRPSPI
                     TVSKASGGDAPRDLYFAGLTLQLANPKTLIFFVAILPQFVDARLPIGAQMVWLAAGSI
                     IPEFFILAGYGWLASRARGIATDVRYARWTDRIAGVLVLGAAALVLSVTA"
BASE COUNT         1136 a         2223 c         2336 g         1244 t
ORIGIN      
        1 ccctgcagga agtggagttt ccggcacgcg tcgctgccta gcgacgacgt gtcgtggttc
       61 agcccgggca ggccttcgcg gcgtcggttt cgcgtttgct gatttcccga tcggccttct
      121 gcatttcatc aatcgttgct gagcgttgct gcagcagtga cgcgcgtgcg gtacgcgcac
      181 gttcgaggtc gcgtttggca cgatcacagc gctcgcgctc cttcgcctga gtttttttct
      241 gctcggctgc ggcacgatcg gccttcgcct cctcggtctc tcgttccttg tcgcgggctt
      301 tctccgcctc cttcgcgttt gccacgtccc gcttggcgcg atccttggct gctttctgct
      361 cctcgacggc gatcggcgga gccgtgttga cctgtcgtac cgacttggtc ccgtcaggac
      421 atgccgcgtt cgagtaagtg accttgccgt ccgcggcctc gcagcgctgc acggtctgcg
      481 cagcggtatg cgtaccggtt gtcgccagcg cggacagaag aatcgccgca cagcgaccga
      541 cgctgtgcgc ggccgggcgg gcagacgcca tcaggggctg catacgcggc ggtactgggc
      601 ggtcagctgg gcggcgtgct ggtccatttg atcgaggtcg tcgagggagt agtagggccg
      661 gttgagcagc gcattacgca ggcggcgaat cgagtcaatc tcgccgcgca gataagcgca
      721 gtcggcaatg cgccgttcct gttgttgctg cgtggctgtc gcctgctgcg cctggcgctg
      781 gtctgcgagc tgctgtgccg cctgggcatc gcgcgccgcc ttttcacgcg cttcacgctg
      841 cgcctcggga ggcggagtcg gggccgcctg cacagccttg atgggcttgg tgccttcagg
      901 acattcggaa ttggagtagg tcacccgtcc gccggcacct tcgcaacgct ggattggctg
      961 cgcggctacg gttgcaggga tgatgaacgc cacagcgcag gcccactgcg cgaatgatcg
     1021 aagcatcgcg cgagtctatc gaacacagca ggtggcgtca ccgcgccccg gccccaggcg
     1081 cggaaccggc ggcgcgtcga agcgcagttc tactgaacgc cgatggcttg tcgcacggcg
     1141 tcggcggcga ccttgttgaa gcgatactgg tagtcgtaca cgctggcacc ggcctggtag
     1201 agcttgccgg gccgcacgcc gggcaggccc ttggtcggct cgatgaatgc gatacgggcc
     1261 gactcgcgtt cgacattgga gacgccggta tcaagcgggt cgacctgcgc cgggaacgac
     1321 agcacgatgg ccttcgtgcc gccaattgtg cgctcttccc actcgccttg ccccacgacc
     1381 tgttcgactg cgcggtcgca gaacagcgtc ccggtcttgg cagggtagag cgatacctca
     1441 cccttcttgc ccggggccgc cgccttgaag tacatcgcgt acggcttggc tccctgccgg
     1501 tcttcgtagg cgagacagta ggggatctgt ttcgagaaat tgccgacgaa ctgcccggta
     1561 ttggtggcgc cggtgaacga ttcccggcgc ggcaacatca gcacgtcgtc gaagaaacgc
     1621 gcagttgtga ggtaggcgac cgcgcctttc gggaagcgta cggtcccggc cttggcggct
     1681 tcctgcttcg gataattttc gggagtgcgc aggaagagcg aaatcggcat gtccgagacg
     1741 tcgtaggcct gcagtgtcac tgccatgcgc agggaggcct gacccgcgta gacgatgtaa
     1801 acggagccat cgttcgcggg aatggcgaag ttcggacgcc ccagtgcacg gcgctcgccg
     1861 tcgagcaccg cgtgcgcgac gtcgatgttg ccgttcagcg tcgtgtggtt ggagtcgttg
     1921 ctcgtttccg acagcgcata gcgattgccc tcaccgggcg tggccgcggc acggatgttc
     1981 gacacgcgcg caccctggaa gtgatacagc ccgtcgccct tgaacgcctc gaaggacggc
     2041 gcggcaatcg gcttggcgcc gggaaccacg accggtttcg gggccgggcc atagaccacg
     2101 acttcggccg gttgctgggc cagcgccagc ggggtggcga gggtcgccag tgccaatgcg
     2161 caggtgcgga gcaaaagtga tgccatgtaa gcctccagtg aattcgaccg cggcaggaat
     2221 ccggccgcgt ttggcgcgaa tggtagtcgc tcggcgaagt tttgatggtc cggagtaggg
     2281 gtggatttcg ctcccatgaa ggtgggactc aagcttcagc cgtccgccgc tatagcacct
     2341 tgcccggatt catgatcccc agcgggtcca gcgcctgctt gagcgtgcgc atcagcccca
     2401 tttcgacttc cgacttgtag cgggcgtttt caccgcgctt gagctggccg atgccgtgct
     2461 cggcggagat cgacccgccg aacgcatgca cctggtcatg cactgtcgaa tacacggctg
     2521 gctgcagcga cagaaaggtc ggttcatcca tgccgatcgg cggactgacg ttgtagtgga
     2581 ggttgccgtc gccgagatgg ccgaaaacca ccatgcgtac accgggaaag cgctgttgca
     2641 gcacgcggtc ggtctcctga acgaaccgcg caatgctgga tatcggcacc gcaatgtcgt
     2701 gcttgatgtt cttgccttcc agcgcctggg cctcggagat cagttcgcgc aacgaccaga
     2761 aggcacgcgc ctgcgcaacc gattgcgcga tgaccgcgtc gctgccgatg ccggcctcga
     2821 tggcgctgcc cgccagtgcc tcgagcagcg cattcgcgtg cgtcgcgtct tcatggtcac
     2881 tgagttccac caggacatgc tggctgtgtc gctgcgcaaa cggcgtgcgg tgtggcgggt
     2941 agtgccgctc caccagcgtc aggcagaagt cgctgaaaca ctcgaacgcg gtcaacgtcg
     3001 ggccgcacgt ggtctgcgcc atggccagca gctgcagcgc ggccggcaac gacggtacgc
     3061 ccagcagcgc ggtagcccgc gcccggggca gtgggaatgt cttgaggcag gcagcggtga
     3121 tcacgcccag cgtgccctcg gcaccgatga agagatccct caggtcgtag ccagtgttgt
     3181 ccttgcgcaa tccgcgcagg ccgtgccaga tctggccgga gggcaacacg acttcgaggc
     3241 ccaggcatag ctcgcgcgcg ttgccgtagc gcagtacctg gacaccgccg gcattggtgc
     3301 tgagattgcc gccgatcgtc gcgctgccct cggcagcgag cgacagcggg aacagctggc
     3361 cctgcatctg cgccgcgtcc tggacgtcct tcagcacgca gccggcttcc gcgatgaggg
     3421 taccgttcgc ggtgtcgacc gaccggatcc gttgcagacg cttgagcgac aggaggatgg
     3481 cccgccccga ttcgtccggc gaggctccgc ccaccaggcc ggtattgcca ccctgaggaa
     3541 cgacaggcgt gcgggtttcg gcgcaccagg cgaccacgcg cgccacttcc gccgtcgagc
     3601 ccggcagaac gacggcctgt gcctgggcgt gatagcggcg ccgccagtcg agcaggaaag
     3661 gtgccttgtc gttggcactc gtcagcacat gcggctcgcc gaccaacgtg cgtagcgcgt
     3721 cgatgtgagt gcgcagcgcg tcgactggac tcacgaggca gacacgcggc gtttcgccgc
     3781 aagcttcatc ggacgcaggt agacgatggc ggccaccaga aacgtcacgg ccagcacgag
     3841 ttcaatcagg gccaggacgg ccgccggtgt cggctcgaac aaacgtgcgg caccctcgaa
     3901 gagatagatc atcacgatga acagcgtcac ctgcatggca taggcgctgc ctcgcagcag
     3961 cgccggcaag ggcagcagca gcgggacgac cttaagcacg agccacgatc cgcccgggcg
     4021 cagcggagcc agcgcaagtt cccaggccag tgccagcgcg atcagcccca gccaggccgc
     4081 gatggcccac cgccacgcgg tgcgttggcg cggcgtggtc tgaggcagtg gtccgatagc
     4141 cgtcctcttc actccgccag ccgcagggcc gtgcgggcca gccgcacacc gagtgcatgc
     4201 gccaggcggc gctcttcgtc ggacagcacc tgcggcgcgc cgtgtcgcgc gacatggctg
     4261 gcaccgtagg gcgtgccacc gctgcgtgtc gccatcaggt cggcttcggt atacggaatt
     4321 cccaggatca gcattccatg gtgcagcagc ggcagcatca tcgaaagcag cgtggtctcc
     4381 tgaccgccgt gcagactgct ggtggagcag aaaacggccg ctggcttgcc ggcgagtctg
     4441 cccgacagcc agagtcccga cgtcgagtcg aagaagtgct tcaggggcgc ggccatgttg
     4501 ccgaagcgcg tcggtgagcc aacggcaaga ccgccgcagg cttcaaggtc agccagttcc
     4561 gcgtacggtg cgccctccga aggcaccggc ggtgcggcga tcgcggtgac tgcagcgacg
     4621 ggcggcaccg tgcgaaggcg cgcctgtgct ccgtcgatgc tgtcgatgcc cgttgcaatg
     4681 gcttccgcca gcctgcgggt ggcaccttgg cgcgagtaga acagaacgag gatgtccacg
     4741 tcgatgcagc gccatggcgc agatgagttg atccggtcac agcggcagta aacacttcgc
     4801 cccgtgagtt cgccggatgt cgccgctatt ataggaatcg tcgtcgcggg ccgccggtgc
     4861 gagacggcac ctgtaggtcc acatcaatgt cccgccttac cgtcgcactc gatcgattcc
     4921 cctggctgaa gactgcgcgc gaagtggccc tcttcgcgta ccgtcgtggc agcgaggtac
     4981 gcctgcagca ggtcgccggc agccttacct ttacgaccgt gctgtccatc gtgcccttgt
     5041 tcgcagtggc acttgcgctg ttctcggctt ttccgctgtt cgccgaattt cgcgagtcct
     5101 tccaggctat ggtgcagcga acgctgcccg cgcagatttc ggccactgtc ctgcgctatg
     5161 tcagcgactt tgcgcagcag gcgacccggc tcacggccgc gggcctcatt ttcctcggcc
     5221 ttacggcgct ggcgatgatc atgacggtcg accgggtgct caacgacatc tggcaggtgc
     5281 gcgatcgtcg gccgttcagc cagcgggtat tgatctactg ggcgctcatc acactcgggc
     5341 cgattctgat cggcgccagc ctgtctgcga gttcatacct ttgggcgctg tcggaggacg
     5401 ccatcgcccg catgccccgg gggctgcgcg ggctgatcga ctttgcgccg gttctcctca
     5461 gtggtctggc ctacgcagcg ctctatgtgt ttgtgccgaa tcgcaccgtg cgctggcgcg
     5521 atgcactgat cggcggcttc gtcgccgcca tcctcgcgga gttgctgaag tccggatttg
     5581 cccactacat cggccgcggt ggagtgcgtt cgatctacgg cgccttcgcg gcgctgccgc
     5641 tgttcctgtt gtggatgtac ctgtcctggt acgcactgtt gttcggtgcg gccatcgcgg
     5701 cgaccgtgcc gcgcctctgg acgacgcgct tcagggacga gttgcgggca ggcgacgact
     5761 ttgtcaccgc cgttgcactg atccagcagc tgcttgcggc gcgcaggcgt ggtaacaccg
     5821 cggtcggcgt cagggtgctt gcgcgcggcg cgcgcaccga tccggttcac gccgagcgcc
     5881 tgctcgagtc cctcgaacgg ctgcgctatg tgcaccggct gtcggcaacg cgtcgccgcg
     5941 gcgaatcatc ggaatggata ctgacgtgcg agccagccag cgcaacgctg cggtccgcgt
     6001 tcgagcgctt cgcgcttgat tcaagcaatt cgctgctgtc gctcgatcgc gagggcgtcg
     6061 gcaacaccct gctcaactgg atcgatcgtg acgagtggat caatgcgccg ctggaacaga
     6121 gcctgtccga tgcgggtgct cgttgaaacc gctcgcagcg gctcgacaag atagtggaac
     6181 agtgaagaag tcgacggacg ttcagctcag gcggtgacgc tgagcaccag cgccgccgct
     6241 ccgagcacga gtacacccgc aatgcggtcg gtccagcgcg cgtagcggac gtccgtggcg
     6301 atgccgcgcg cgcgcgaagc gagccagcca tagcccgcga ggatgaagaa ttccgggatg
     6361 atcgaacccg ccgcgagcca gaccatttgt gcgccgatcg gcaggcgcgc gtcgacgaac
     6421 tgcggcagta tcgcgacgaa gaagatcagc gttttcggat tggcaagctg gagcgtgagt
     6481 ccggcaaagt agagatcgcg cggcgcgtcg ccgccggagg cctttgagac ggtgatcggg
     6541 gagggccgtc cgaatatcgc ggatacgccg agatagacga ggtaggccgc gccgatccac
     6601 tttacggcgg tgaagaactc gcgcgacgcg gcgagcactg cgccgagcgc cgttgcggaa
     6661 acagcgaagt agaacgcgtt ggcactgaga atgccgaggc tcgcgtaggt cgaccggcgt
     6721 gcgccatggg cgagtccaca ggcgatgacc agcatcaccg ccggtccggg cgaaagcgac
     6781 agcgcaatct cggtaaggac gtagagccag tacgtgtccc agttgacggc gtgcagcaat
     6841 gtgcgccctc aaccggaatc aaaccgtcgc cggcgcgaac acccgggcag cgaactgtgc
     6901 cagcgcgccc agcacgtcat cggggcgctc ggccatcag
//
LOCUS       DATIRT010000503         1593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_876641, whole genome shotgun sequence.
ACCESSION   DATIRT010000503 DATIRT010000000
VERSION     DATIRT010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876641"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            384..1358
                     /locus_tag="VLN25_11570"
     CDS             384..1358
                     /locus_tag="VLN25_11570"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSQ11230.1"
                     /translation="MIERRRFIGTLAGGLLASPFGAFAQLPTKVPRIGVLGNTDSPVW
                     EGFRQGMRELGYIDGRNVTMEWRWLEGRSDRLPAQALELVQLNVDIIVATSTQSVRAA
                     MQATSRTPIVMGLSAYPDKIGLVGSLARPGGNVTGLSNIAPELMGKRFQLLKEVAPKV
                     SRVAVLWNPASPVEPFGFSDTMVAAAANGVEIKSIEVRTPEDYPAAFTTVTASRADAL
                     YVFSNPVTFKHRQLIADFALKSRLPSICEEKSFVESGGLLSYGPSFFELLRRSANFVD
                     KILKGAKPADLPVEQPTKFELVINLKTAKALGVTIPQSLLLRADEVIQ"
BASE COUNT          302 a          481 c          492 g          318 t
ORIGIN      
        1 cacgtaacga cgcgccgtga cacgcaatgg cacgtctttc gggcggaacg atcgcgggtc
       61 cagtccctcg ccgcgcatcg aacttcgggg cttccgcgga cttggtctat ggtaccgccc
      121 gcagcgcgcg gtgacatcga aggcgcggag gtcccaagcc gattgacgaa ccgcgcctgt
      181 tcgataatgt taacgacatc ctactgccgt tgcgctacgc gttgacaacg acagcaacgg
      241 cgcgatttcg gacgtagcgt ttggcgcggc gtcggtcgct tatcggccag aaccagccgc
      301 tcagcgttgc cgtccgaatt gccgggcagc ggtcattgcg aagcgtagta tggcgatctg
      361 cagttcatgg acgaggtgat tcggtgattg aacggcgcag gttcatcggc acactcgctg
      421 gcggtcttct cgcctcgccc ttcggggcct ttgcgcagct gcccacaaag gttccccgca
      481 ttggagtcct cggcaacacg gacagcccag tgtgggaggg ctttcgccaa ggaatgcgcg
      541 aactgggtta tatcgatggc cgcaatgtca cgatggagtg gcgatggttg gagggaaggt
      601 ccgatcggct ccccgcgcaa gcgctcgagc tcgtccaatt gaacgtcgac atcatcgtcg
      661 cgacgagcac gcagtcagtc cgggccgcca tgcaggcgac gagcaggact ccgatcgtga
      721 tggggctgtc cgcttatccc gacaaaatcg gcctcgtagg gagcctcgcc cgccccggcg
      781 gcaacgtcac gggcttgagc aacattgcgc cggaactgat gggcaagcgc tttcagcttc
      841 tcaaggaggt ggctccaaaa gtctcccgtg tggccgtgtt gtggaatccg gcgagcccgg
      901 tcgagccgtt cggattcagt gacactatgg tggccgcggc tgcgaatggc gtggagatca
      961 aatcgatcga ggtgcgcaca ccagaggact accccgcggc attcacgacc gtgactgcga
     1021 gccgtgccga tgccttgtat gtttttagca accccgtcac cttcaagcac cggcagctca
     1081 tcgcggattt tgccttgaag agccgacttc caagcatttg cgaggaaaag agtttcgtcg
     1141 agtctggcgg tctattgtcc tacgggccaa gtttctttga gttgctccga cgctctgcga
     1201 acttcgtcga caagatcctc aagggtgcga agccggccga ccttccggtc gagcagccca
     1261 ccaagttcga gctcgtgatc aacttgaaga ccgccaaggc gctcggcgtg accatcccgc
     1321 aatcactgct gctgcgcgcg gacgaggtga tccagtaatc gaacattcgg gattgttcca
     1381 ggcattccgt tcgcgcacct ctgcgcggcg cggctgaacc ccaatattga atggctgctc
     1441 ttgcaaacgt cgaccggctg gagtgggtcg gtagttcgcg tccggccagc ggcatctgcg
     1501 cgccactctg ccacatgctc acgttatatg ggacccctcg atctgaagag tacacggcgg
     1561 cgcaaacatt gcgacggttg atcactgcag agt
//
LOCUS       DATIRT010000504         2688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1859868, whole genome shotgun sequence.
ACCESSION   DATIRT010000504 DATIRT010000000
VERSION     DATIRT010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1859868"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VLN25_11575"
     CDS             complement(<1..541)
                     /locus_tag="VLN25_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11231.1"
                     /translation="MKLQQISESCFAVLNEKGRLCDSNSGFINRGGGVLVDTQSDLSH
                     ARRMAELFGSIWQGVPKRVVNTNESGDHVWGNQVFSGAEIIAHRTVPDRMMQAATPRE
                     FQALLGGFDGLLSRLMLKATRPGVLAIGRQLGQDYDFDGIEPTLPATLFDERFVLDLE
                     GLEVHMIHVGPCHRVGDTIV"
     gene            718..1716
                     /locus_tag="VLN25_11580"
     CDS             718..1716
                     /locus_tag="VLN25_11580"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSQ11232.1"
                     /translation="MKHPPFTSTQPAVSVVEISDPTAVGAGIELIEQDAVQLQEMSLR
                     ARRVIVRLGASAVVFYSTNRRVRTRTSARKGLLAYVMFGPKSSGTVNGLPVSAGLMLA
                     AAPDSEAIFVADAGYESITFLLSPDEIRAHLTARQRESEFRVPRGVESLQVDRDSARK
                     LFEWGKRLVDTAARQPALFNERETERNAAHVELVELLLGVLGGAHDFEPSQSDRNRQA
                     QTLIVKIAEEFALSQSDDHLYVSDLCKVAGVSERTLEYAFKAVMRLTPVTYLIRLRLH
                     RVRRVLLAATQGSTTVSTEALNWGFWHFGEFSRAYKDCFGELPSDTLRRKPGELQT"
     gene            complement(2254..>2688)
                     /locus_tag="VLN25_11585"
     CDS             complement(2254..>2688)
                     /locus_tag="VLN25_11585"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HSQ11233.1"
                     /translation="VLDTPPEERFDRLTRLAAAIFDVPTALVSLVDRDRQWFKSAHGS
                     EARETPREISFCAHAVASKAPMVVPDALADPRFAENPMVKGAPRVRFYAGQPLILPDG
                     NCVGTVCLVDTRPRDLDQRAMGLLRDIAELVRRELLLTDAKA"
BASE COUNT          504 a          846 c          857 g          481 t
ORIGIN      
        1 gaacaatcgt gtccccgacc cggtggcatg gcccgacgtg aatcatgtga acctccaagc
       61 cttcgagatc cagcacgaat cgttcgtcga acagcgtcgc cggaagcgtc ggctcgatcc
      121 cgtcgaagtc gtagtcctgc cccagctgcc taccgattgc tagtacgccc ggacgggttg
      181 ccttgagcat tagacgagag agcaagccat cgaagccacc gagcagcgct tggaattccc
      241 tcggggtggc agcctgcatc atgcggtccg gcaccgttcg atgcgcgata atctcggcgc
      301 ccgaaaagac ctggttgccc cacacatggt caccgctctc gttggtattg accacgcgct
      361 tcggcacgcc ctgccagatg ctgccgaaca actcagccat tcgacgcgcg tgggacaggt
      421 cagattgcgt gtcgacgagg acgccgccgc cgcggttgat gaatcccgag ttcgagtcgc
      481 atagacggcc cttctcgttc aagaccgcaa agcaactttc gcttatttgt tgcaatttca
      541 tagggcgccg cgtccgaagt atcgacttgc gtttcgatga acaattgaag tctggcaacg
      601 gcaccgtgaa taccagctga tccggaccgg agcctctgcc tcgctggaac tacagccact
      661 gctacacttt cccgacttgg cacgtagtcg tctatcggaa ttccgcagaa cgcattcgtg
      721 aagcaccctc cgttcaccag cacacagcct gcagtttcgg tcgtcgagat cagcgatccc
      781 acggcggttg gcgccggtat tgagctgatc gagcaggatg ctgtacagct ccaggagatg
      841 tcgcttcggg cgcgccgagt gattgttcgt ctcggggcgt ctgcggtcgt tttctattcg
      901 accaaccgtc gcgtgcggac gcgcacgagc gcccgcaagg gcctgcttgc ttacgtcatg
      961 tttggaccaa aatcgagcgg gactgtgaac ggactaccgg tgagcgcagg cttgatgctc
     1021 gcggcagcgc ccgattcgga ggccatcttt gtagccgacg ccgggtacga gagcattacg
     1081 tttctgcttt cgccagacga gatccgtgcg catctcacgg cccgccagcg cgaaagcgag
     1141 tttcgtgtgc cgcgcggcgt ggaatcgctg caggtcgacc gcgatagtgc taggaagcta
     1201 ttcgagtggg gcaagcgact ggtggatact gccgcacggc aaccggcact tttcaacgag
     1261 cgggagacgg aacggaacgc ggcccacgtc gagttggtcg agcttctatt gggagtcctt
     1321 ggcggggcac acgacttcga gcctagccag agcgaccgga accggcaggc gcagactctc
     1381 atcgtcaaga tcgccgaaga gtttgccttg tcgcaatccg atgatcacct ctacgtcagc
     1441 gacctgtgca aggtcgccgg agtgagcgag cgcaccctgg agtacgcatt caaggcggtc
     1501 atgcgattga caccggtgac ctacctgatc cgactccgat tgcaccgcgt gcgccgggtg
     1561 ctcctggcag cgactcaggg atcaaccacg gtgtccaccg aggcgctcaa ctggggcttc
     1621 tggcacttcg gagagttctc acgcgcctac aaggattgct ttggtgaact cccatcggac
     1681 accctgcgac gaaagccggg agagctgcag acatgaatcg tggcgccgac gctgcgtaga
     1741 cgcggcaata atgcaatcgg accacggagg gaaaccctaa gcagcaatgc gctgccgccc
     1801 actgcattca gtccggagtc ctcgcccggc atacgttgca cggccggcca gtggacaacg
     1861 cttgaggact gcatgctctg aacgtcagct ttcagagcct tggatgacag cttccgggtt
     1921 gctcgaactt ctgcaacggg tcgaaagcgc cagttcgtcc agctgtagcc accggctgca
     1981 ctcagctctg cagcagccgc ttcgacacga atgctcgacg ttcgggctag gccgaaccgt
     2041 gacctacgca cagcgcggga cctgaacggg aacgagcggc ggcggcaagg agcgcggtcg
     2101 gggcgcggcg gcggcggtgg ttatgcaacc attaccctgc aatttgccga gccgggtact
     2161 cggaggagag ctgcctgacg cccccggacc cgtgggtgtg tcgggcacgc cggccgatgg
     2221 cagcgaagtg gcactccggc ggcacgtccc cgactacgcc ttggcgtcgg tcagcagcaa
     2281 ttcgcggcgg acgagctcgg cgatgtcgcg cagcagcccc atcgcccgtt ggtcgaggtc
     2341 gcgcggccgg gtgtcgacga ggcacacggt gcccacgcaa ttgccgtcgg gaaggatcag
     2401 cggctgcccg gcgtagaagc gcacccgcgg cgcgcccttg accatcggat tctcggcgaa
     2461 tcgcggatcg gcgagcgcat cggggacgac catcggcgcc ttgctcgcca cggcgtgcgc
     2521 gcagaacgag atttcgcgcg gcgtctcgcg cgcctcggag ccgtgcgccg acttgaacca
     2581 ctggcgatcg cggtcgacga ggctgacgag cgccgtcggg acgtcgaaga tcgccgcggc
     2641 cagccgcgtc aggcggtcga accgctcctc gggcggcgtg tcgaggac
//
LOCUS       DATIRT010000505         2500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1466774, whole genome shotgun sequence.
ACCESSION   DATIRT010000505 DATIRT010000000
VERSION     DATIRT010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466774"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VLN25_11590"
     CDS             <1..504
                     /locus_tag="VLN25_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--glyoxylate aminotransferase"
                     /protein_id="HSQ11234.1"
                     /translation="WKEMLAANVNGYFPYTPATNLLYGLHEALDMLFAEGLPQVYARH
                     DRFAEATHRAVHAWGLEILCVNPAEYSSALTAVLMPEGHNADAFRKIVLERFDMSLGQ
                     GLGKVSGRIFRIGHLGYFNDLMLCGTLAGVEMGLALAGVPHRKGGVDAAMAYLAEAPA
                     AALAHAA"
     gene            601..1611
                     /locus_tag="VLN25_11595"
     CDS             601..1611
                     /locus_tag="VLN25_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HSQ11235.1"
                     /translation="MTFKHLDAARRRTLLRTALAAAVATGLFAATAASAQQEIKLGHV
                     GEPGSIFQKSSDEFARRANAKLGSKAKVVVYGSSQLGGDKEMIQKVKLGTMDMAVPST
                     VMSSEVDLFGIFEMPYIVKDRKHMAKIEKDVFWPLLAPAAEKKGVKVIAVWENGVRHI
                     TNNKKPIKAPADLQGIKLRVPEGKWRVKMFQAYGANPSPMKFSELFTALQTGVMDGQE
                     NPFTQIYSAKLQEVQKFLSLSGHVYTPAYVITGKTWSSLPPDVRQILEDTAKETQAFV
                     YETAAKDEDELLTKLKAGGMQVNEVDKDAFVKASTSIYEEFGKEVPGAKEAIDKSVAL
                     GK"
     gene            1669..2187
                     /locus_tag="VLN25_11600"
     CDS             1669..2187
                     /locus_tag="VLN25_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HSQ11236.1"
                     /translation="MRRFRARYGKLLEWVVIGLMVILAAEVTLGVVFRTIGKSLSWYD
                     ELASVLLAWLTFYGSALASVKRAHIGCPEVVEQMPWRLRRAFSIFAQLLVIAFFTLLG
                     FVGAQILPILHGESLVSLSWVPMSVVQSVIPISSALIVVAEVTHLIDLVIAREPPHRP
                     ASSGPALADGLH"
     gene            2199..>2500
                     /locus_tag="VLN25_11605"
     CDS             2199..>2500
                     /locus_tag="VLN25_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HSQ11237.1"
                     /translation="MIIMLLLFAAVLALVLINVPIAVSLGIVALVAMVSSHGLVSLPN
                     LPLVAYNGATSFPLLAIPLFILAGAIMNASGISQRLIAFASALFGWIRGGLAHVSIG"
BASE COUNT          476 a          769 c          797 g          458 t
ORIGIN      
        1 tggaaggaga tgctggcggc caacgtcaac ggctacttcc cgtacacacc ggcgaccaac
       61 ctgctctatg gattgcacga agcgctcgac atgctgtttg ccgaaggtct gccacaggtc
      121 tacgcgcgac acgaccgttt cgccgaggcg acgcaccggg cggtgcatgc atggggcctc
      181 gagatactgt gcgtcaatcc ggccgaatac agctccgcgc ttactgctgt gctgatgccg
      241 gagggccaca acgccgacgc gttccgcaag atcgtgctcg aacgattcga catgtcgctg
      301 ggccagggac tgggcaaggt gtcgggccgg atcttccgca tcggacacct tggctacttc
      361 aacgacctga tgctgtgtgg cacccttgcc ggtgtcgaga tgggcctggc gctcgcgggc
      421 gtgccgcatc gcaagggcgg cgtggatgcc gcgatggcgt atctcgccga ggcgcccgcc
      481 gcagcactcg cgcacgcggc ctaacgggac gagacagagg gaagaggagc ggcgcgcccg
      541 tccgagcgtg ccgacaagga cagaatcgcg gacacaaaag ccataaccaa ggagacgacc
      601 atgactttca aacatcttga tgccgcgcgc cgccgtaccc tgctgcggac ggcactggcc
      661 gccgccgttg caacgggact cttcgccgcg acagcggcca gcgcccagca ggaaatcaag
      721 cttggccacg tcggtgagcc cggctcaatc ttccagaaga gttcggacga attcgcgcgc
      781 cgcgccaatg ccaagctcgg tagcaaggcc aaggtcgtgg tctacggttc gagccagctc
      841 ggcggtgaca aggagatgat ccagaaggtc aagctcggca cgatggacat ggcggtgccc
      901 tcgacagtca tgagctcaga agtggatctg ttcggcatct tcgagatgcc gtacatcgtc
      961 aaggaccgca agcacatggc gaagatcgaa aaggacgtct tctggccgtt gctcgctcca
     1021 gccgcagaga agaagggcgt gaaggtaatc gccgtctggg aaaacggcgt gcgccacatc
     1081 accaacaaca agaagcccat caaggcaccg gccgacctgc agggaatcaa gctgcgtgtg
     1141 cccgaaggca agtggcgcgt gaagatgttc caggcctacg gtgccaatcc gagccccatg
     1201 aagttctcgg agcttttcac cgcgctgcag accggcgtga tggacgggca ggagaatccg
     1261 ttcacgcaaa tctattcggc gaagctgcag gaagtgcaga agttcctgtc gctgtcgggc
     1321 cacgtctaca cgccagccta tgtgattacg ggcaagacct ggagttcgct gccgccggat
     1381 gtgcgccaga ttctggagga cacggccaag gagacgcagg cctttgtcta tgagacggcg
     1441 gccaaggacg aggatgaact ccttaccaag ctcaaggccg gtggcatgca ggtcaacgaa
     1501 gtcgacaagg atgcgttcgt caaggcgagc acgtcgatct acgaagagtt cggcaaggaa
     1561 gttccgggag ccaaagaagc gatcgacaag tccgtcgcac tgggcaaatg aacggtgagc
     1621 gcagataacc agactggcgg caccgccgag gcgccgctcg cggaattctt gcgtcgtttc
     1681 cgcgcgcgct acggcaagct actcgagtgg gtcgtcattg ggctgatggt gatcctcgcg
     1741 gccgaggtga cactcggcgt cgtgttccgc accatcggca agtcgctgtc ctggtatgac
     1801 gaactcgcct ccgtactgct ggcctggctg accttctacg gctctgcgct cgcgtcggtt
     1861 aaacgtgcgc acatcggctg cccggaggtg gtggagcaga tgccgtggcg cctgcgccgc
     1921 gcattcagta tcttcgcgca gctcctcgtg atcgcattct tcacgctgct cggtttcgtc
     1981 ggcgcacaga tcctgccgat ccttcacggc gaatcgctgg tcagtttgag ctgggtgccg
     2041 atgagtgtcg tacagtcggt catcccgatc agctctgcgc tgatcgtcgt ggccgaagtg
     2101 acgcatctga tcgatctcgt gatagcacgc gagccaccgc accgacctgc gtcctccggc
     2161 ccggcgctcg ccgacggcct gcactaagcg gagagctcat gatcatcatg ctgctgctct
     2221 tcgccgcggt gctggcgctg gtgctgatca acgtgccgat tgcagtgtcg ctcggcatcg
     2281 tcgcactggt cgcgatggtg tcgagccacg gcctggtgtc gctgcccaac ctgccactgg
     2341 tcgcctacaa cggtgccacc agctttccgt tgctcgcgat tccgctcttc atcctggccg
     2401 gcgcgatcat gaacgcgtcc ggcatctcgc agcggctgat cgcgtttgcc tcggcgctgt
     2461 tcggttggat tcgcggtggg ctcgcgcacg tctcgatcgg
//
LOCUS       DATIRT010000506         3679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1172394, whole genome shotgun sequence.
ACCESSION   DATIRT010000506 DATIRT010000000
VERSION     DATIRT010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172394"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VLN25_11610"
     CDS             complement(<1..519)
                     /locus_tag="VLN25_11610"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HSQ11238.1"
                     /translation="MKTIGLIGGMSWESTVPYYRLINEEIKERLGGLHSAKVILYSVD
                     FHDIERLQHSGDWDAAGTLLAGAAQTVEKAGAELLVLCTNTMHKVAPAIEAAVRIPLL
                     HIADATAREIMAAGISTVGLLGTRFTMEQGFYKDRLQRNRGLRMLIPDERQREVVHRV
                     IYDELCVGKIVDD"
     gene            complement(560..2191)
                     /locus_tag="VLN25_11615"
     CDS             complement(560..2191)
                     /locus_tag="VLN25_11615"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HSQ11239.1"
                     /translation="MAVAKHVLPADFPDPLERRRRIEAEPLPANVWQLLEDAARAVPD
                     QLLWDFFQSASRATYAEVRDMAAALTQGLSRRGVRKGTVVAVMLPNVPEYPLSWLAAA
                     RLGAVVVPLNPQYTAREVRYVLEDAGATFAIVHHTCAAVVESALDPQGQLGRERIFVV
                     GAATGDRTPFSVLLEGRPDELAKDASPGLNDLLNIQYTSGTTGLPKGCLLTHRHWLTC
                     GKVNARRDGRVYRRILASTPFFYMDPHWLLLMTLFQRATLFVAERQSASHFMEWVRRH
                     GIEFCLFPELVLKQPPSTADGNNALVRMNIYGVKPERHAEIERRFNVIAREAYGMTEM
                     GSVMFMPMESTDMVGSGSCGLPSPFRECRIVDSDGTVLGTGEMGELQVRGPGAMLGYH
                     HKPEANEAAFDGEWFRTGDLFRKDERGFYYLVGRLKDMIRRSGENVSAQEVERVLRGM
                     PEVLEAAVIGVPDETRGEEILAIVVPVEGATVEPDHLFSTCAESLARFKVPRFLRVET
                     ILPKTPSGKIAKGQLRDRWTESRTGCYDRVVQAWL"
     gene            complement(2182..>3679)
                     /locus_tag="VLN25_11620"
     CDS             complement(2182..>3679)
                     /locus_tag="VLN25_11620"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSQ11240.1"
                     /translation="HFFESDERATYREVRRAVNRLANGLHTRGVRKGTKVAVMLPNVA
                     AMPKTWLALARLGAIMVPVNIAYTPRELEYVVTDSDATYLVIHEECLPALDGLAQLPG
                     AITADTVFVVESARSGRFQSWHNLCRDQPETLTFQVAIDLDDLMNIQYTSGTTGFPKG
                     CMLSHRYWLLLAKVQGEHDGRTYTRVMAANPFFYMTPQWLLLMCFYSRGTLFVARRLS
                     GSRYLSWIREHRIQFCLFPEAAYKQPPKPDDADNEIIRVSTYGFPRQEQLRLEERFDF
                     VAREAFGMTEIGTALFVPIEAADMTGLGSCGVAEPFRECRIGDEKGDTLPIGATGELL
                     IRGPGILQGYYRNPEATAQAFHGDWFRTGDLFRQDERGYFYIVGRVKDMIRRSGENIA
                     ARELEQVVMGLPTVAEAACIPVPDPHRGEEIKLLVVLQPEVSRDECTPTTILAHCRAN
                     LAPFKVPRYISYVDALPKTGSAKIAKAELRAAANLTAGCYDALVDRWL"
BASE COUNT          682 a         1199 c         1112 g          686 t
ORIGIN      
        1 gtcgtcgacg atcttcccaa cacacagctc gtcgtaaata acgcgatgaa cgacttcgcg
       61 ttgccgctca tcagggataa gcatgcgaag tccacggttg cgctggagcc gatccttgta
      121 gaagccttgc tccatcgtga atcgagttcc gagcaggccg acggtggata tgcccgcggc
      181 catgatctcc cgggcggtcg catcggcgat atgcagcaat ggaatacgca cggctgcttc
      241 gatcgcgggg gcgaccttgt gcattgtgtt ggtacacagc accaggagtt ccgcgccggc
      301 cttctcgacc gtttgcgcgg cgcccgccag caaggtccct gccgcgtccc agtctcctga
      361 gtgctgcagc cgctcgatgt cgtgaaagtc aacgctgtaa aggatgacct tcgccgagtg
      421 cagcccgccc agccgttcct tgatctcctc gttgatcaac cggtagtacg ggaccgtcga
      481 ctcccagctc atgccaccga tcaggcctat ggtcttcacg aagagtcctc agttcagagt
      541 tggacagaga tctgtcgcgt caaagccagg cctgaacaac ccggtcatag cagccggttc
      601 gggactctgt ccaccgatcg cgcaactggc ctttagcgat ctttccggag ggtgtcttgg
      661 gcaagatcgt ctcgacacga aggaagcgcg gaactttgaa gcgtgcaagc gactcggcgc
      721 aggtcgagaa gaggtgatcg ggttccacag tggctccctc gaccgggacc acgatggcaa
      781 ggatttcttc cccgcgcgtt tcgtccggca ccccgatgac tgcggcctcc agcacctcgg
      841 gcatgccgcg caaaacgcgc tcgacttcct gagctgacac gttctccccg ctgcgacgaa
      901 tcatgtcctt caggcgcccg acgaggtagt agaacccgcg ttcgtccttc cgaaagagat
      961 cacccgtgcg gaaccactcc ccgtcaaatg ctgcttcgtt tgcctccggc ttgtggtggt
     1021 agcccagcat cgctccaggc ccccgcacct gaagctcacc catttctccg gtaccgagca
     1081 ccgtgccgtc ggaatcgacg atccggcact cgcgaaaggg agatggaagg ccgcacgaac
     1141 ccgaacccac catgtcggtc gactccatcg gcatgaacat gacggaaccc atttccgtca
     1201 tgccatacgc ctcgcgcgca atcacattga agcgccgttc gatttcggca tgccgctcgg
     1261 gtttgacgcc atagatgttc atgcggacca atgcgttgtt gccgtctgcg gtggatggtg
     1321 gctgcttgag gacgagttcc gggaacaggc agaactcgat cccgtgccgc cgaacccatt
     1381 ccatgaagtg actcgcgctc tggcgctcgg ccacgaagag cgttgcgcgc tggaacaacg
     1441 tcatcagcag cagccagtgt ggatccatat agaagaacgg cgtcgaggcc aggatccgcc
     1501 gatagacgcg gccatcgcga cgcgcattca ccttcccaca ggtcagccaa tgccggtgcg
     1561 tcagcaggca tcccttgggc agccccgtcg ttcccgaggt gtattgaatg tttagcaggt
     1621 cattgagtcc ggggctcgcg tccttcgcca actcatccgg acgtccttcg agcaagactg
     1681 agaacggcgt ccgatcgccc gtcgctgcgc caaccacgaa gatgcgctct cgaccgagct
     1741 gaccctgcgg gtccagtgcg ctttcaacga ccgctgcgca cgtgtggtga actattgcga
     1801 aggtcgctcc ggcatcctcg agcacgtacc ggacctcgcg cgcggtgtac tgagggttca
     1861 gcgggaccac caccgctccc aggcgtgcgg ctgcaagcca gctcagcggg tactccggca
     1921 cgttgggcag catcaccgcc acgaccgttc ccttccgaac acctcgccgc gacaatcctt
     1981 gcgtcagcgc cgccgccatg tcgcgcacct cggcataggt cgcgcgggac gcggactgga
     2041 agaagtccca gagcaattga tccggcaccg cacgcgccgc gtcctcgagc agttgccaga
     2101 cgttggccgg caatggctcg gcctcgatgc ggcgacgtcg ttcgagcgga tctgggaaat
     2161 ccgcaggaag gacgtgtttg gctacagcca tcgatccacc aacgcgtcat agcagcccgc
     2221 cgtcaggttc gcagccgcgc gcagttcggc cttcgcgatc ttggccgacc cggtcttggg
     2281 cagcgcgtcg acatagctga tgtagcgcgg gaccttgaac ggggccaggt tcgcacggca
     2341 atgcgcaagg attgtcgtcg gcgtgcactc gtctcgtgac acctcaggct gcagcacgac
     2401 cagcagcttg atctcttcgc cgcgatgcgg atcgggcacg ggaatgcacg ccgcctctgc
     2461 gactgtcggg agtcccatga cgacctgctc caactcgcgc gcggcgatgt tctcgccgct
     2521 gcggcgaatc atgtccttta cccgcccgac gatgtagaaa tagccgcgct catcctgccg
     2581 gaagagatct ccggtacgaa accagtcgcc gtgaaacgcc tgcgccgtcg cctcgggatt
     2641 cctgtagtag ccctgcagga ttcccgggcc gcggatcagg agttcgccgg tcgcaccgat
     2701 gggcaacgtg tcgcccttct cgtcgccgat gcggcactcg cgaaacggct cggccacgcc
     2761 gcacgagccc aggccggtca tgtcggccgc ttcgatcggc acgaagagcg cggtgccgat
     2821 ttcggtcatg ccgaatgcct cgcgcgcgac gaagtcgaag cgttcttcca gccgaagctg
     2881 ttcctgtctc ggaaagccgt aggtgctcac ccggatgatc tcgttgtccg cgtcatcggg
     2941 cttcggcggc tgcttgtagg cggcttccgg gaagaggcaa aactggattc gatgctcgcg
     3001 tatccacgag aggtagcgac tgccgctcag gcgccgcgca acgaacaggg tgccgcggct
     3061 gtagaagcac atcaacagca gccactgcgg cgtcatgtag aaaaacggat tggccgccat
     3121 gacacgcgtg taggtgcggc cgtcatgttc gccctgcacc ttggccagca gcagccagta
     3181 acggtgcgac agcatgcagc ccttcgggaa ccccgtcgtg cccgacgtgt actggatgtt
     3241 catcaggtcg tcgaggtcga tagcgacttg aaaggtcagt gtctccggct gatcccgaca
     3301 gaggttgtgc caggactgaa accgaccgct acgcgctgat tccacgacga acaccgtgtc
     3361 cgccgtgatc gcaccgggca actgtgccag cccgtccagt gccggcaggc actcctcatg
     3421 aataaccagg taggtcgcgt cgctgtcggt gacgacgtac tcgagttcac ggggcgtgta
     3481 ggcgatgttg accgggacca tgatggcccc gagccgcgcc agcgccaacc atgtcttggg
     3541 catggctgcg acgttgggca gcatgacagc gaccttcgtg cctttgcgca cgccgcgcgt
     3601 gtgcaagcca ttggccaaac ggttcaccgc ccggcgaact tctctatagg tcgcgcgctc
     3661 gtcgctttcg aagaagtgc
//
LOCUS       DATIRT010000507         2877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1368666, whole genome shotgun sequence.
ACCESSION   DATIRT010000507 DATIRT010000000
VERSION     DATIRT010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368666"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..401)
                     /locus_tag="VLN25_11625"
     CDS             complement(123..401)
                     /locus_tag="VLN25_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11241.1"
                     /translation="MNLRIVVFAVITSLPIAVAMAAPAADQPAQKPTAEQTHRNEDIA
                     KHRQMARAHEEAAKCLEAGGTEKQCQERLREACKGIAVGQYCGMRHAH"
     gene            complement(404..1603)
                     /locus_tag="VLN25_11630"
     CDS             complement(404..1603)
                     /locus_tag="VLN25_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSQ11242.1"
                     /translation="MNAFRLVIAQLRRRPLQSALGVVLLALGIATLVFVILVQSQLTH
                     QLERDARNVDLVVGAKGSPLQLVLSAVYQVDVPTGNVPLATVGLLRGNRLIAQAVPLS
                     MGDSFRGFRIVGTEPLFLELHDATVAAGRLWSEPMEAVIGAEVARTAQFGPGSEFLGT
                     HGLAEGGAAHEDARYRVVGVLAPTGSVIDRLVLTDKASVWRVHEGEAADETERRILEA
                     EREVTAVLVRYATPMGAAIVPRQVNALDRLMAASPANEIARLFAVVGVGIETMRGFAW
                     ILVAMSLLALFVTLFNALEERRYDVAIMRLLGASQARVAGLLLLEAWLLAFISVVLGL
                     ALGLGAVALVGQWLAQARAFTLSASATAPALGLIVAVAFVVATLAAAVPTWRASRMDV
                     SKALAQG"
     gene            complement(1600..2229)
                     /locus_tag="VLN25_11635"
     CDS             complement(1600..2229)
                     /locus_tag="VLN25_11635"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSQ11243.1"
                     /translation="MLSTGAIVHRFGDSTVLRAPPIELLADEHCALVGPSGSGKSTLL
                     NVLAGILRPSAGSVRLDSEDLYPLHGRDDRWRGLRIGMVPQRLHLLAGLTAFENVALA
                     CYFAGTSQNGVAPLLTELGIAQRSSARPAALSVGEQQRVAIARAMVNRPRLLLADEPT
                     SSLDDDNAQQALDLLLAAAQRGGALLIVATHDARIRGRFQRQIELTRPA"
     gene            complement(2334..>2877)
                     /gene="dksA"
                     /locus_tag="VLN25_11640"
     CDS             complement(2334..>2877)
                     /gene="dksA"
                     /locus_tag="VLN25_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HSQ11244.1"
                     /translation="VPIALAPVSAKAVPAPSPKDLAAAHEYLLARSATRSEEPVLVNG
                     KRLPSEGELLKMSESAYMNEQQLAFFKYRLQQMERELLQNADETTEHLRETVIVPDPA
                     DRATIEEEHALELRTRDRERKLLKKVQQSLASIESGDYGYCEETGDPIGIPRLLARPT
                     ATLSLEAQQRREIKQKMYGD"
BASE COUNT          500 a         1007 c          895 g          475 t
ORIGIN      
        1 cgcagcctgt cccttcattc caatctcctt gagcgcaccc accgcgcccg gtccctgccc
       61 gacgggcacc gctcccgcac gtcgctttgc atcagcatcc gatcgtccag cccgtgcaga
      121 cctcaatgcg cgtgccgcat cccgcaatat tgacccaccg cgatgccttt gcaagcttcg
      181 cgcagccgct cctgacactg cttttcagtg cctcccgctt ccaggcactt cgctgcttct
      241 tcgtgcgcac gcgccatctg cctgtgcttg gctatgtctt cattgcgatg ggtctgctcg
      301 gccgtgggtt tctgcgccgg ctgatcggcc gccggcgcgg ccatcgccac tgcaattggc
      361 aaagaagtga tcaccgcgaa aacgacgatg cgaagattca tgctcacccc tgtgcgagag
      421 ctttcgatac gtccatccga ctggcgcgcc atgtcggcac cgccgcagcc agcgtggcga
      481 ccacaaaggc caccgcaaca atcaggccca gcgccggcgc ggtcgcggag gccgacagcg
      541 tgaaggcgcg cgcctgcgcc agccattgac cgacgagtgc aacggcgccc agtccgagcg
      601 caagccccag cacgacggag atgaacgcca gcagccaggc ttccagcagc agcagccccg
      661 ccacacgcgc ctggctggcc cccagcaacc gcatgatcgc gacatcgtaa cgccgctctt
      721 cgagcgcgtt gaacagcgtg acaaacagcg ccagcaacga catggcgacc agtatccagg
      781 caaacccgcg catcgtctct ataccgaccc cgaccacggc gaacaggcgt gcgatctcgt
      841 tggccggcga cgccgccatc agccgatcca aggcattgac ctgacgtggc acgatcgccg
      901 ctcccatggg cgtggcgtag cgcaccagca ccgcggtgac ctcgcgttcg gcttcgagga
      961 tcctgcgctc ggtttcatcc gcggcttcgc cctcgtgcac gcgccacaca cttgccttgt
     1021 cggtcagcac cagtcggtcg atgacggacc cggtcggagc cagcacgccg acgacccgat
     1081 acctcgcatc ctcgtgcgct gcgccgcctt cggccagtcc gtgggttccg aggaattcgc
     1141 tcccagggcc aaactgcgcc gtgcgagcca cctcggcgcc gatcaccgct tccatgggct
     1201 cactccagag ccgacccgcc gcgaccgtgg cgtcgtgcag ttcaagaaac aacggttcgg
     1261 tgccgacgat gcgaaagccg cggaagctgt cgcccatgga caacggaacg gcctgcgcga
     1321 tcagtcgatt gccgcgcagc agcccaaccg tggccagcgg cacgttgccc gttggaacat
     1381 cgacctgata gaccgccgac agcacgagtt gcaacggtga acccttggcg ccgaccacga
     1441 gatcgacgtt gcgcgcgtcg cgctcgagct gatgcgtcag ttgcgactgg accaggatga
     1501 cgaagaccag cgtggcgatg ccgagggcca gcagtacgac gcccagggcc gactgcagcg
     1561 gccggcggcg caactgggcg atgacgagcc ggaacgcgtt catgccggac gggttagttc
     1621 aatctggcgc tggaatcgcc cgcggatgcg ggcgtcgtgc gtggcgacga tcagcagggc
     1681 gccgccccgt tgcgcggcgg cgagcagcag atcgagggcc tgctgagcat tgtcatcgtc
     1741 caggctcgac gtcggctcat cggccagcag caggcgtggt cgattgacca tcgcgcgcgc
     1801 gatcgcgaca cgctgctgct cgccgacgct caacgcggcg ggccgcgctg acgatcgctg
     1861 cgcgatgccg agctcagtca gcagcggcgc cacgccgttc tgcgacgtcc cggcgaagta
     1921 acacgcgagc gcgacattct cgaatgcggt cagtccggcg agcagatgca gcctttgcgg
     1981 caccatcccg atgcgcagcc cgcgccaccg atcgtcgcgg ccgtgcaatg gatacaggtc
     2041 ttcgctgtcg agccggaccg aacccgcgct cggccgcagg atgccggcca gcacgttgag
     2101 gagtgtgctc ttgcctgaac cgcttggacc caccaacgcg cagtgctcgt cggcaagcag
     2161 ttcgatcggc ggcgcgcgca gcaccgtcga gtcaccgaag cgatgaacga tggcaccggt
     2221 cgacagcatc gatcagtccc gcaaccgcct tgtcgactga gggaggaatt ccaaagaggg
     2281 ggcagcccct ctttcatcct caagagtgga tgggcagggt cagacgggaa gcgtcagtcc
     2341 ccgtacatct tttgcttgat ctcgcgccgc tgctgggcct ccagtgacag cgtcgcggtc
     2401 ggacgcgcca gcaggcgcgg aatcccgatc ggatcaccgg tttcctcgca gtagccgtag
     2461 tcgcccgact cgatgctcgc cagcgactgc tgaaccttct tcagcagctt gcgttcgcgg
     2521 tcacgggtcc gcagttcgag cgcatgctct tcttcgatag tcgcgcgatc ggcaggatcg
     2581 ggcacgatga ccgtctcacg caggtgctct gtcgtctcgt cggcattctg gagcagttcg
     2641 cgttccatct gctgaagccg gtacttgaag aacgccagtt gctgctcatt catgtacgcc
     2701 gactcggaca tcttcagcag ttcaccctcg ctcggtaggc gcttgccgtt caccagcacc
     2761 ggctcttcgc tgcgcgtggc cgaccgggcc agcaggtact cgtgggcagc ggcgagatcc
     2821 ttggggctgg gcgccggaac cgccttggcc gaaacggggg cgagggcaat cggcacg
//
LOCUS       DATIRT010000508         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1368901, whole genome shotgun sequence.
ACCESSION   DATIRT010000508 DATIRT010000000
VERSION     DATIRT010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368901"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(409..1758)
                     /locus_tag="VLN25_11645"
     CDS             complement(409..1758)
                     /locus_tag="VLN25_11645"
                     /inference="COORDINATES: protein motif:HMM:NF020322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSQ11245.1"
                     /translation="MSHHAADLLVGPCDGPRYSGGARNAENLMGEWLQSVLGSNTLLS
                     GGLTLALLGLLAVWLRDLPGKVFGWAKHFLITTLTVDSRDELMFPALVEYMDSREALR
                     RLNNFTARAVRQGTSYQSLHDELQQGGRPLTVLSPGEGFHVFFLDGRLMWMKREVQVG
                     PSIFEKIALSAFGRDKQPLEEIVHAAMEHRIARELNRIAVYMPSPYNNEWMRARLGNN
                     RKLDSVVLKAGQKESILEDLNRFFASHERYEALGIPWRRGYLLYGQPGTGKTSLVTAI
                     ASELALNVCVLSLASPTVTDEKIGNLLSSVPQRSVILIEDVDAFFRQRDKADAGVKVS
                     YSGFINALDGVAAHEGSVVFLTTNHPELIDEAAIRSGRVDFRLELGLCDASQLERMFL
                     KFFDDASAAARFASEVAPGLWSPAQVQERLLKAESVDEAIALFRDTKPAATLPSKAA"
     gene            1811..>2102
                     /locus_tag="VLN25_11650"
     CDS             1811..>2102
                     /locus_tag="VLN25_11650"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HSQ11246.1"
                     /translation="MLNSIRDFFDRHIGASPAPADERHSIQLATAALLVEVARIDREW
                     TSDERAAVQRAVREKFDLSPDEADNLIDLAEAEVQQATDYFQFTSLVNRHFTQ"
BASE COUNT          388 a          688 c          644 g          382 t
ORIGIN      
        1 gcacccatca cgatggcggg cacgaggcca cgcgcagcat cgagatccgg cgtatcgccg
       61 ttctcagtct tcttcatata catgacctct acgacgcttg tggcatcgcc ctcggcttgg
      121 tcctgatcag gacattcgga gcggttggct cctgttctta tttcgcttgc ccgactgcgc
      181 gcgcgagtgc ggcgccggaa tcggcggccg cacattgaga ccggaaattc taaggatcga
      241 tcgtcaactt cgctcgagtc acggctctag tttcctcccg tgcaggcggt aacggatcaa
      301 ttcatgtgtc gagaattgtg caaggacggc ccgtcagccg cacgatggcc gcgatgcaac
      361 gacgcgtgac actcagctcc gaccaagtcc atgcctgcgg tcgtcgactt acgcagcctt
      421 cgacggcaag gttgccgcgg gcttcgtgtc gcgaaacagc gcgatcgctt cgtcgacact
      481 ctcggccttg agcagtcgct cctgcacctg cgccggcgac cacagtcccg gggcgacctc
      541 gctcgcgaaa cgggctgccg cggaggcgtc gtcgaaaaac ttgaggaaca tgcgctccag
      601 ctggctggcg tcacacaagc cgagctcgag ccggaagtcg acccgcccgg agcgaatcgc
      661 tgcctcgtcg atcagctccg gatggtttgt cgtcaggaac acgacagagc cttcgtgcgc
      721 agccacgccg tcgagcgcgt tgatgaagcc ggagtacgag accttgacgc ccgcgtccgc
      781 cttgtcgcgc tgtcggaaga aggcgtcgac atcctcgatc aggatgaccg agcgttgcgg
      841 cacggatgac aacaggttgc cgatcttctc gtccgtgacg gtcggtgatg cgaggctcag
      901 cacacagacg ttgagcgcaa gttccgatgc aatggcggtt accagcgagg tcttgccggt
      961 gcccggctgg ccgtaaagca gatagccgcg ccgccacgga atgccgagcg cctcgtagcg
     1021 ctcgtgcgag gcgaagaagc gattcaggtc ctccaggatg gattccttct ggccggcctt
     1081 gagcaccacc gagtcgagct tgcggttgtt gccgagccgc gctcgcatcc actcgttgtt
     1141 gtagggactc ggcatgtaga ccgcgatgcg gttgagctcg cgcgcgatgc gatgttccat
     1201 cgccgcgtgc acgatttcct cgagcggttg cttgtcgcgg ccgaaggccg acagtgcaat
     1261 cttctcgaag atcgagggcc cgacctgcac ctcgcgcttc atccacatca ggcgcccatc
     1321 gaggaaaaaa acatgaaagc cctcgcccgg tgacagcacg gtgagcggtc gccctccctg
     1381 ctgcagttcg tcgtgcaggc tctggtagct cgtgccctgg cgcaccgcgc gcgcagtgaa
     1441 gttgttgagc cgtcgcagtg cctcacgact gtccatgtac tccaccagcg ccggaaacat
     1501 cagctcgtcg cgactgtcga cggtcagcgt cgtgatcaga aagtgcttgg cccagccaaa
     1561 aaccttgccc ggaagatcgc gcagccagac ggcgagcagg ccgagcaggg ccaatgtcag
     1621 cccgccggac agcaatgtgt tcgagcccaa gacgctctgc agccactcgc ccatcaggtt
     1681 ctccgcgttg cgtgcgccgc cactgtagcg cggcccgtcg cacgggccga ctagcaggtc
     1741 cgcggcgtga tgcgacacta gaatctcatt tcccggcagt cgtcggggat tccgtcacag
     1801 gtgcaagcga atgctgaatt caatccgcga tttcttcgac cgccacatcg gcgcatcccc
     1861 ggcaccggcg gacgaacgcc attccatcca gctcgccact gccgcgctgc tggtcgaagt
     1921 cgcgcgcatc gaccgcgagt ggacgtcgga cgagcgcgcc gccgtgcagc gcgcggtacg
     1981 cgaaaaattt gacctgtcgc cggacgaggc agacaacctg atcgacctgg cggaggccga
     2041 agttcagcag gccaccgact acttccagtt cacatcgttg gtcaaccgcc acttcacgca
     2101 gg
//
LOCUS       DATIRT010000509         2008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_877250, whole genome shotgun sequence.
ACCESSION   DATIRT010000509 DATIRT010000000
VERSION     DATIRT010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877250"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1368
                     /locus_tag="VLN25_11655"
     CDS             <1..1368
                     /locus_tag="VLN25_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HSQ11247.1"
                     /translation="YVAQASTTGHATNIIAGIAVSMKACAWPVIAICAAIYASYWLAG
                     LYGIAVAATSMLSMTGIIVALDAYGPITDNAGGIAEMADMPKEVRAITDPLDAVGNTT
                     KAVTKGYAIGSAGLAALVLFADYTESLRTAGLATTFDLSNHLVIIGLFIGGLIPFLFG
                     AMAMEAVGRAASSVVEEVRRQFREIPGIMEYKAKPDYSRAVDMLTSAAIKEMMIPSIL
                     PVLVPIVVGVLLGPAALGGVLMGTIVTGLFVAISMTTGGGAWDNAKKYIEEGHHGGKG
                     SDAHKAAVTGDTVGDPYKDTAGPAVNPLIKIINIVALLIVPIIATMHGGKPATAAATA
                     PTATIAVPVAVPVVVVIGAPMRVHFDSGSDSISTAGKLIVAGYAQALKANPQAAIELS
                     GYADPSGDAAKNLELAKHRAMVVRDELVAKGVAAERVTLVKPTDVIVGQGSDADARRV
                     DIVLR"
     gene            1380..1901
                     /locus_tag="VLN25_11660"
     CDS             1380..1901
                     /locus_tag="VLN25_11660"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HSQ11248.1"
                     /translation="MFDEEDREIGYALAIAVALAIFISIFTLSIAAGSAIGQFGKKAG
                     SGASAMALPVAGATAATATTAAALPDATTVKLYFELGTAELPGDALTRLAPLITAAKG
                     APASVLVLSGYHDASGDPVANAELAKQRAFAVRDLLATAGVSERGIELAKPDVTTGGA
                     DPREARRVDVTLR"
BASE COUNT          353 a          676 c          653 g          326 t
ORIGIN      
        1 tatgtcgccc aggcctcgac gacgggccac gcgaccaaca tcatcgccgg cattgcggtg
       61 tcgatgaagg catgcgcctg gccggtaatc gccatttgcg cggcgatcta cgcttcttac
      121 tggctcgcgg gcctgtatgg catcgccgtt gcggccacct cgatgctgtc gatgaccggc
      181 atcatcgtcg cgcttgacgc ctatggcccg atcaccgaca acgccggcgg cattgccgag
      241 atggcggaca tgccgaagga agtgcgcgcg atcaccgacc cgctcgatgc cgtcggcaac
      301 acgaccaagg cggtcaccaa gggctacgcg atcggctcgg caggcctcgc cgcgctggtg
      361 ctgttcgctg actacaccga aagcctgcgt accgctggcc tggcaaccac gttcgacctg
      421 tcgaaccacc tggtgatcat cggacttttc atcggagggc tgattccgtt cctgttcggc
      481 gcgatggcga tggaagcggt cggccgcgcc gccagcagcg tggtcgaaga agtgcggcgc
      541 cagttccgcg agatccccgg gatcatggaa tacaaggcca agcccgacta cagccgggcg
      601 gtcgacatgc tgacttcagc ggcgatcaag gaaatgatga tcccgtcgat cctgcccgtg
      661 ctggtgccga tcgtggtcgg cgtgctgctc ggacccgccg cactcggtgg cgtgctgatg
      721 ggcacgattg tcacgggact tttcgtggcc atttcgatga ccacgggcgg cggcgcgtgg
      781 gacaacgcca agaagtacat cgaggagggg caccatggcg gcaagggctc ggacgcacac
      841 aaggcggccg tgaccggcga caccgtcggc gatccgtaca aggacacggc cggccccgcg
      901 gtcaatccgc tgatcaagat catcaacatc gtggcactgc tgatcgttcc gatcatcgcg
      961 acgatgcatg gcggcaaacc ggccacggcg gcggccaccg cgccgacggc cacgatcgcg
     1021 gtcccggtcg ccgttcccgt cgtggtcgtc atcggcgcgc cgatgcgcgt gcatttcgac
     1081 tccggctcgg attccatctc gacggcaggc aagctcatcg tcgccggcta cgctcaggcg
     1141 ctcaaggcca atccgcaggc tgcgatcgag ctgtccggct atgcggatcc atccggcgat
     1201 gcagccaaga acctcgaact cgccaagcac cgcgcgatgg tggtccggga tgaactggtc
     1261 gccaaaggcg tggccgcaga gcgcgtgacg ctggtcaagc cgaccgacgt catcgtcggc
     1321 cagggcagcg acgccgacgc gcgccgcgtt gacatcgtgt tgcgttaacc ggagatcgca
     1381 tgttcgacga agaagacagg gaaatcggat acgcgctcgc catcgcggtc gcattggcaa
     1441 ttttcatttc gatcttcacc ttgagcatcg ctgccggcag cgcgatcggc cagttcggca
     1501 agaaggccgg ctcaggcgca tcggccatgg cgcttccagt tgccggtgcg acggcggcaa
     1561 ccgcaaccac cgcggcggcc ctgccggacg ccacaacggt caagctctac ttcgaactgg
     1621 gcacggccga actgcccggc gatgcgctga cccggctagc accgttgatc acggccgcca
     1681 agggggctcc ggccagcgtt ctggtcctgt ccggctacca cgatgccagc ggcgatccgg
     1741 tcgccaacgc cgagctggcc aagcagcgtg ccttcgcggt acgcgacctg ctcgccaccg
     1801 cgggcgtcag cgagcgtgga atcgaacttg caaagcctga cgtcacgacc gggggcgccg
     1861 atccgcgcga agcgcgccgt gtcgacgtga ccctgcgctg accggcgaga cgtacacacc
     1921 ggggcggtcg gctcgcgagg taccgccctt tttttctgat ccacccgccg atggaactgc
     1981 ggaccgcgca accagccgac gtcgcgtc
//
LOCUS       DATIRT010000510         4721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1762890, whole genome shotgun sequence.
ACCESSION   DATIRT010000510 DATIRT010000000
VERSION     DATIRT010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1762890"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VLN25_11665"
     CDS             complement(<1..375)
                     /locus_tag="VLN25_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSQ11249.1"
                     /translation="MSSYVSPWMSSELEVYRDTLRRFVETEIVPHEDRFAKQQHVDRA
                     LWNKAGELGVLCADIPDEYGGAGGNFAHMAALFDELAAAGDTSFGVHVHIIVAHYLLN
                     QGTEEQKRKYLPQLASGAMVGAI"
     gene            complement(498..1712)
                     /gene="dinB"
                     /locus_tag="VLN25_11670"
     CDS             complement(498..1712)
                     /gene="dinB"
                     /locus_tag="VLN25_11670"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HSQ11250.1"
                     /translation="MSPTRVIAHLDMDAFYASVELLRYPDLRGRPVVIGGGRDHQPVW
                     ETDPASGEQRRRYATLRSYAGRGVVTTATYEARALGVNSAMGMMKAAAKAPDAVLLPV
                     DFDEYRKYSRLFKAAIRAVAPLIEDRGIDEIYIDLTEVVAARLPDEDELIDPDDAAQN
                     VWWCAREVGAELRQVVRDATGLTCSVGLAPNKLIAKIASELDKPDGLTVVTQKEMASR
                     LWPLSVRRLHGVGPKSAARLARLGIASIGELAQADRAWLAGHFGASYASWLADAAQGR
                     DDRPIVTESEPRSISRETTFDRDLHATRDRAQLSRIFTHLVERVADDLRRKRYSGRTI
                     GIKLRFEDFSTVTRDQTLAEPTSDARAIRRAAGQCLKRVDLTRRIRLLGVRIGSLEPV
                     DEASAGTPARLV"
     gene            1913..3634
                     /locus_tag="VLN25_11675"
     CDS             1913..3634
                     /locus_tag="VLN25_11675"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HSQ11251.1"
                     /translation="MSTVPAVAHTVAEWVADFLVAHGVDRVFGLQGGHIQPIWDHLAL
                     KGVRIIDVRDEGAAVHMAHAHAVLSGGLGVASVTAGPGVTNCVTAMANAQLERVPVLL
                     IGGCAPVPQDDLGPLQGIDHVSILRPVTRSARTLRVAGSLLRDLDKAVSTALGDGNAP
                     GPVYVEIPTDVLRQTVPPRVVLPEYLAPRAQRRIPPDPAEVERAAALIAGAKRPLVLT
                     GRGAVTARAELMQFLDESGAAYLDTQESRGLVPPEHSSTIGAMRGRAMQETDLAIVVG
                     RKLDYQTGYGSPAVWPKANFLRIGDNAEELRENRRGEVELFAHPALALAALARALPAH
                     RSARDPAWCEALRSEHLRRSQKYAAALAEAPAGKDGRMHPNQIFAALREVMRPDAITV
                     ADGGDLLSFARVGLESSTYLDAGAFGCLGVGTPYGVAAALLYPDRPVVAVTGDGAFGI
                     NAMEIDTAQRHGAKVVFIVSNNAAWNIERVDQVMNYGGRVVGTELAWSDYAAMARAFG
                     LHGERVTDPARLAGAFREAFEKAPALIDVVVTRDALSSDAGKGLAFAGDFQALSAWDD
                     AERARRA"
     gene            3631..>4721
                     /locus_tag="VLN25_11680"
     CDS             3631..>4721
                     /locus_tag="VLN25_11680"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSQ11252.1"
                     /translation="MSRQSTARDSHAAAAGDPRSGVLNVGQIVAAHARLQPDKIGARD
                     TKRALTFAQWNERSNCLANALLGLGLAKGDRVALLAYNCIEWLELYVALAKAGLVAVP
                     VNFRLTPPEIEYIVANCEARAMIVQDALLESIEPVRAHLSLIVKDAWVHFGGPRTPRG
                     WRGYESLVRAGSARVPAVEVNPADTWALMYTSGTTGRPKGAIRNHHGAALISLVTALD
                     MELTRQDTALLVMPMCHANSLYFSFTFTYLGATCVVDDRRSFDPEALLHSMAEQRVTF
                     TSLVPTHYIMMLGLPPQAKARHDVSSMRKLMISSAPARRDTKLAILEHFRNSQLYELY
                     GSTEAGWVTLLRPDEQITRLGSVGREWTGS"
BASE COUNT          741 a         1597 c         1608 g          775 t
ORIGIN      
        1 gatcgccccc accatcgcgc cgctggcaag ctgcggcagg tacttgcgct tctgctcctc
       61 ggtgccttgg ttcagcaggt agtgcgcgac gatgatgtgc acgtgcacgc cgaacgaggt
      121 gtcgccggcc gcggcgagtt catcgaagag cgctgccatg tgcgcaaagt tgccgcccgc
      181 gccgccgtac tcgtcgggaa tgtcggcgca cagcacgccc agctcaccgg ccttgttcca
      241 tagcgcgcga tcgacatgct gctgcttcgc gaagcgatcc tcgtggggga cgatctcggt
      301 ttcaacgaag cggcgcaggg tgtcgcgata gacctcgagt tcggacgaca tccagggcga
      361 gacgtaggac gacatggcgc gggaatccgg cgtgatgcga acctgatcaa cctagcacga
      421 ctgcgcgacc gtgactcgcc gtcgacgtac tccggactag acgagagtct cgaacgcgcg
      481 ccttccggca catcggctca gacgagacgg gctggcgtgc ccgcggacgc ttcgtccacg
      541 ggctcgagcg acccgatccg cacgccgagc aggcgaatac gacgtgtgag gtcgacgcgc
      601 ttcaggcatt ggccggccgc gcggcggatg gcccgggcgt cgctggtcgg ctcggcgagc
      661 gtctggtcgc gcgtgacggt gctgaagtcc tcgaagcgca gcttgatgcc gatcgtgcgg
      721 ccggaatagc gcttgcggcg caggtcgtct gcgacccgtt cgaccaggtg ggtgaagatg
      781 cgggacagtt gcgctctgtc gcgcgtggca tgcaggtcgc gatcgaaggt ggtctcgcga
      841 ctgatcgagc ggggctcgct ctcggtcacg atcggacggt catcgcgccc ctgcgcggcg
      901 tcggcgagcc acgacgcata gctggcgccg aagtgtcccg cgagccaggc gcgatcggcc
      961 tgcgccagtt cgccgatcga ggcaattccg agccgcgcca gccgggccgc cgacttgggc
     1021 ccgacgccat gcaggcgccg taccgacagc ggccacaggc gcgacgccat ctccttctgc
     1081 gtcacgacgg tcaggccgtc gggcttgtcc agctccgacg cgatcttcgc aatcagcttg
     1141 ttcggtgcca gtccgaccga acaggtgagc ccggtggcgt cgcgcacgac ttgcctcagt
     1201 tcggcgccga cctcgcgcgc gcaccaccag acgttctgcg cggcatcgtc ggggtcgatg
     1261 agttcgtctt cgtccggcag ccgcgccgcg acgacctcgg tcagatcgat gtagatctcg
     1321 tcgatgccgc ggtcctcgat cagtggagcg acggcccgaa tcgccgcctt gaacagccgc
     1381 gagtatttgc ggtactcgtc gaagtcgacc ggcagcagca ctgcgtccgg tgccttcgcg
     1441 gcggccttca tcatgcccat cgccgagttg acaccgagcg cgcgggcttc gtaggtggcc
     1501 gtcgtcacca cgccgcgccc ggcgtagctg cgcaaggtcg cgtagcgacg gcgctgttcc
     1561 ccgctggccg ggtcagtctc ccagacaggc tgatgatcgc gcccgccgcc gatcaccacc
     1621 ggccgtccgc gcaggtccgg atagcgcaac agttccaccg acgcgtagaa cgcatccatg
     1681 tcgaggtggg cgatgacgcg agtcgggctc atgtcggccg caagactacc ggctgtcgcg
     1741 gcgttgcgat gcggcacgtc cgatgcgcga cggcggcggg ttctgccccc tagtcgagtc
     1801 gcaggtcccg agcccataat cgccgctccc tcgggacggc gggcagcaac tcacttcgac
     1861 ccgtccttca gttccgccgg caagtcgtca tcgcaagcac aggaggcaga ccatgagtac
     1921 ggttcccgcg gttgcccaca ctgtcgccga atgggtggct gactttctgg tggcgcacgg
     1981 cgtcgatcga gtgttcgggc tgcagggcgg ccacatccag ccgatctggg accacctggc
     2041 tctcaagggc gtgcgcatca tcgacgtgcg cgacgaaggg gctgctgtgc acatggcgca
     2101 tgctcatgcg gtgcttagcg gcggcctggg tgtcgcctcg gtcacagcag ggccgggagt
     2161 gacgaactgt gtgacggcaa tggccaatgc gcagctcgag cgagtaccgg tgctgctgat
     2221 tggtggttgc gcgccggtgc cccaggatga tctcggcccg ctgcagggca tcgatcatgt
     2281 gtccatactg cgtccggtca cccgcagcgc gcgcacgctg cgcgtcgccg gcagcctgtt
     2341 gcgcgacctg gacaaggccg tgtcgaccgc actgggcgac ggcaacgcgc ccggtcccgt
     2401 gtatgtcgag attccgacgg atgtgctgcg gcagaccgtg cccccgcgcg tcgtgctgcc
     2461 ggaatacctg gcaccgcgcg cgcagcggcg gatcccgccc gatccggccg aggtcgaacg
     2521 tgcggccgcg ctgatcgcgg gtgcgaagcg cccgctggtc ctcaccggcc gcggtgctgt
     2581 gacggcgcgc gccgagctga tgcagttcct cgatgagtcg ggcgcggcct acctcgatac
     2641 gcaggaaagt cgcggactgg tgcctcccga gcattcgtcg acgatcggcg cgatgcgtgg
     2701 ccgtgcgatg caggagacag acctcgcgat cgtcgtcggc cgcaagcttg actatcagac
     2761 cggctatggc tcaccggctg tctggccgaa ggcgaacttc cttcgcatcg gtgacaacgc
     2821 ggaggagttg cgcgagaacc ggcgcggcga ggtcgagttg ttcgcgcacc cagcgctggc
     2881 gctggcggcg ctggcacggg cactgccggc acacagatcg gctcgcgatc cagcgtggtg
     2941 cgaagccctg cgcagcgagc acctgcggcg ctcgcagaag tacgccgccg cgctggctga
     3001 agctcccgcc ggcaaggacg ggcgtatgca tccgaaccag atcttcgccg cgctgcgcga
     3061 agtgatgcgg cccgatgcga tcactgtggc cgacggcggc gacctgctga gttttgcgcg
     3121 ggtcggactt gagtcgagca cctatctcga tgccggtgcc ttcggctgtc tcggcgtcgg
     3181 cacgccttac ggcgtggcgg ccgcgctgct gtatccggac cgcccggtcg tcgccgtcac
     3241 cggcgacggc gcgttcggca tcaacgcgat ggagatcgac acggcgcaac ggcacggcgc
     3301 caaggtcgtg ttcatcgtct cgaacaacgc cgcctggaac atcgaacggg tcgaccaggt
     3361 gatgaactac ggcggccgcg tggtcggcac cgaactggca tggagcgact atgcggccat
     3421 ggcgcgcgca ttcggcctgc acggcgaacg cgtgaccgat cccgcgcgac tcgctggcgc
     3481 cttccgggag gcgttcgaga aagctccggc tctgatcgac gtggtcgtta cacgtgacgc
     3541 gctgagttcg gatgcgggca aggggctggc gttcgctggc gacttccagg cactgtcggc
     3601 ctgggacgac gccgaacgcg cgcggcgagc atgagccggc aaagcacggc acgcgactcg
     3661 cacgccgctg cggcaggcga cccgcgaagc ggcgtcctca acgtcggcca gatcgtcgcg
     3721 gcgcacgccc ggctgcagcc ggacaagatc ggtgcgcgcg acacgaagcg tgcattgacc
     3781 ttcgcgcaat ggaacgagcg cagcaactgc cttgccaatg cgttgctcgg ccttggcctc
     3841 gcgaagggcg accgcgtcgc gctgctggcc tacaactgca tcgaatggct tgagctctac
     3901 gtcgcgctcg ccaaggccgg gctggtcgcg gtgcccgtca acttccggct gacgccgccg
     3961 gagatcgaat acatcgtcgc caattgcgag gcgcgcgcga tgattgttca ggacgcgctg
     4021 ctcgagagca tcgagcccgt gcgcgcccat ctttccctga tcgtcaagga cgcctgggtg
     4081 cacttcggcg gcccgcgcac tccccgcggc tggcgcggct acgaatcgct ggtgcgcgcc
     4141 ggctcggcgc gcgtccccgc cgtggaggtg aacccagccg atacctgggc gctgatgtac
     4201 acctcgggca ccaccggccg accgaaaggg gcgatccgca accatcacgg cgctgcgctg
     4261 atctcgctgg tcacggcgct cgacatggag ctgacacggc aggacaccgc gctgctggtg
     4321 atgccgatgt gccacgccaa ctcgctctat ttcagtttca ccttcacgta cctcggtgca
     4381 acctgtgtcg tcgacgaccg ccgcagcttc gatccggaag ccctgctgca ttcgatggcc
     4441 gaacagcgcg tcaccttcac gtcactggtc ccgacgcact acatcatgat gctcggcctg
     4501 ccgccgcaag caaaagcgcg acatgacgtg tcgagcatgc gcaagctgat gatctcttcc
     4561 gcgccagcgc ggcgcgacac caagctcgcg atcctcgagc acttccgcaa ttcacagctc
     4621 tatgaactgt atggctcgac cgaggccggc tgggtcacgc tgctgcgtcc ggacgaacag
     4681 atcacgcgcc tcggctcggt cggacgcgag tggaccggct c
//
LOCUS       DATIRT010000511         2703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_90447, whole genome shotgun sequence.
ACCESSION   DATIRT010000511 DATIRT010000000
VERSION     DATIRT010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90447"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..778)
                     /gene="lolA"
                     /locus_tag="VLN25_11685"
                     /pseudo
     CDS             complement(197..778)
                     /gene="lolA"
                     /locus_tag="VLN25_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631288.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
     gene            complement(823..>2703)
                     /locus_tag="VLN25_11690"
     CDS             complement(823..>2703)
                     /locus_tag="VLN25_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HSQ11253.1"
                     /translation="YNLKAALPGAPGGIIGNAIGSGVQAAIGFTGASVIFLVAIALGL
                     SLFLDFSWLAVAERLGTFIEGFSRRAEQRREEAEDRAIGEAATKLREAALAEERERID
                     EAPPVHIERPPAHVKPSERKQKEKQVPLFAEMADGRLPALDLLDEPPPAVENVSTETL
                     EFTSRLIEKKLRDFNVEAQVVAAYPGPVITRYEIEPAVGVKGSQIVNLAKDLARSLSL
                     VSVRVVETIPGKNLMGLELPNSRRQVVKLSEIMGSAVYNESASLVTLGLGKDIAGKAV
                     VVDLARMPHLMVAGTTGSGKSVGINAMILSILYKADPSQVRLILVDPKMLELSVYEGI
                     PHLLCPVVTDMREAGNALQWCVAEMDKRYRLLSKLGVRSLSGYNQKLADARAKGEILK
                     NPFSLTPEDAEPLEPLPMIVVVIDELADMMMVTGKKVEELIARIAQKARASGMHLILA
                     TQRPSVDVITGLIKANIPARISFQVSSRVDSRTILDQMGAESLLGQGDMLYLAPGTGL
                     PTRVHGAFVSDAEVHRVVETLRKRGVPQYVEGVLEAPTTEGDESGGGQPGGEADPLYD
                     QAVEIVVKNRRASISLVQRHLRIGYNRAARLLEDMERAGLVSSMQSNGNREILVPSRD
                     GG"
BASE COUNT          451 a          914 c          856 g          482 t
ORIGIN      
        1 tccaggaggg gagggcaatg acaaaacaac cgccggctcg gtgcatcgag gggtagggtg
       61 cgcgcccttg ttcttcggcg gagcgcgcgg ccgctgcgga gcgcagccga ctaacgagtg
      121 ttgcgcggtt cgcgcaatgc gcgaaccgta tcgccgcgag ctcacgtgcg cccgcagggc
      181 gcgtaagtga gcggcgttac tccgtcagga catccgcccc cttcggcggc gtgaagcgga
      241 acacatcggc gtcgagcttg gggttgcgct cgacccgtga gaaagtgagg cgggtcgtct
      301 ggccaaagct gtcgacgagc agcatggcgc cgggcagccc gtcgcgaaag ccgatctcga
      361 tgcgttcgaa cggcgtgtcc ttcgcgcgcg gtttggcctg gacccattcg agtccctcgc
      421 gcgcgccagc atcgctgacc acgaactcgc gctcgaattc attggatccg aacaggatcg
      481 acgcgggact cgacggcaag gccgacgcga gcttgcggat ggtcacctgg ttgagatcct
      541 tgtcgaacat ggtcagttgc tggccgtcgg caatgatcaa ctgctcgtac ggcttctcgt
      601 agatccagcg gaacttgccc ggacgctgga acacgaaacg tcaactcgac tgctgtgttg
      661 ccgcgtcgcg aaccacgcgc tgcacgaact cgccgcgcgc gcttgatgtc ttcgtcagga
      721 agttgcgcaa ctggtcgata gagaccgcat gcgcctgcgc ggcgacgaac acagccatcc
      781 atatcaccca tctgcaggtt tgaagcacgt gttccttagt cgttatccgc cgtcgcgcga
      841 cggcaccagg atctcgcggt tgccgttgct ctgcatcgac gaaaccagtc cggcccgctc
      901 catgtcctcg agcaggcgcg cggcacggtt gtagccgatg cgcaggtgcc gctgcaccag
      961 cgagatcgac gcccggcggt tcttgacgac gatctcgaca gcctggtcgt ataacggatc
     1021 agcctcacct cccggttgac cgccgccgct ttcgtcgccc tcggtggtcg gcgcttccag
     1081 cacgccttcc acgtactgag ggacgccgcg cttgcgcagg gtctcgacga cgcgatgcac
     1141 ctcggcatcc gatacgaacg ctccgtgaac ccgcgtcggc aacccggtgc ccggcgcgag
     1201 gtacagcatg tcgccctggc ccagcaagga ctccgccccc atctggtcca ggatggtgcg
     1261 cgaatcgacc cggctcgaca cctggaacga aatccgcgcg gggatgttgg ccttgatcag
     1321 cccggtgatc acgtcgaccg agggtcgctg cgtcgccagg atcaggtgca ttcccgaggc
     1381 acgtgccttc tgggcaatgc gcgcgatcag ttcttcgacc ttcttgccgg taaccatcat
     1441 catgtcggcg agttcgtcga tcacgacgac gatcatcggc agcggctcca gcggctcggc
     1501 gtcttccggc gtcagcgaaa acgggttctt caggatctcg cccttcgcgc gcgcgtcggc
     1561 cagtttctgg ttgtagccgc tgaggctgcg cacaccgagc ttgctcagca gccggtagcg
     1621 cttgtccatc tcggcgacac accactgcag cgcgttgccc gcctcgcgca tgtcggtcac
     1681 gaccgggcac agcaggtgcg gaatgccctc gtagaccgag agttcgagca tcttcgggtc
     1741 aaccaggatc agcctcacct gcgacgggtc ggccttgtac aggatcgaca ggatcatcgc
     1801 gttgataccg acggacttgc ccgagcccgt ggtgccggcc accatcaggt gcggcatccg
     1861 tgccaggtcg accacgaccg ccttgccggc gatgtccttg cccagcccga gcgtgaccag
     1921 cgacgcgctc tcgttgtaga ccgccgagcc catgatctcc gacagcttga cgacctggcg
     1981 gcgtgaattc ggcagctcca gtcccatcag gttcttgccg ggaatcgtct cgaccacgcg
     2041 caccgacacc agcgacaacg agcgagccag gtccttggca aggttcacga tctggctgcc
     2101 cttgacgccg accgccggct cgatctcgta gcgcgtgatt accggccccg ggtaggcggc
     2161 cacgacctgg gcttcgacat tgaagtcgcg cagcttcttt tcgatcaggc gtgacgtgaa
     2221 ctccagggtc tctgtcgaca cgttttcgac ggccggggga ggttcgtcca gcaggtcgag
     2281 cgcgggcagt cgcccatcgg ccatttccgc gaacagcggg acctgctttt ccttctgctt
     2341 gcgctccgac ggcttgacat gcgcaggcgg ccgttcgatg tgcacgggtg gcgcctcgtc
     2401 aatgcgttca cgctcctccg ccagggccgc ctcgcgcagc ttcgtcgccg cctccccgat
     2461 cgcgcgatcc tcagcctcct cacggcgctg ctcggcgcgg cgcgagaagc cttcgatgaa
     2521 cgtgccgagg cgctcggcga ccgccagcca cgagaagtcg agaaacagcg acaggccgag
     2581 cgcgatggcc acgaggaaga tcacgctggc cccggtaaag ccgatcgccg cctgcacgcc
     2641 agagccgatg gcattgccga tgatgccgcc gggcgctccc ggcagcgccg ccttcaggtt
     2701 gta
//
LOCUS       DATIRT010000512         5914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_188346, whole genome shotgun sequence.
ACCESSION   DATIRT010000512 DATIRT010000000
VERSION     DATIRT010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188346"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..554
                     /locus_tag="VLN25_11695"
     CDS             60..554
                     /locus_tag="VLN25_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11254.1"
                     /translation="MSLTPRRGCRAMRLTGLALLAAFMPLSSAMAQSEGGLYIAGDAG
                     FKFRQAAERGLAQNPGGRRFFVLAVPPQTSALKKSATGPLAQLRDRVIAGNGVLFVCQ
                     RDIDKGRIDPAELVPGVVAVRGFPPPGSNAIPAGERYFPDEDRSRLPASNEALRRLRA
                     TCSS"
     gene            complement(632..2842)
                     /locus_tag="VLN25_11700"
     CDS             complement(632..2842)
                     /locus_tag="VLN25_11700"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HSQ11255.1"
                     /translation="MHARTVDSRRLSMVLVAVAAFAGASHAAPVAVTVPPPLPAQPVV
                     DNHWGVAVPDPYRFLEDTKDPKVQSWLKAQANATDSVLLSIPGRAGLLTRMKEIEAAA
                     AGLATDVVRTEGNRFFFERRNPTDGQFKLVWRDGIDGADTVIVDPEALSKAGGRPHAI
                     MDFAPSRDGRFLAYSIQVGGGEIGTLHVIDVASGKPVIEPIDRIRYASTAWLEDGSGF
                     FYSRLIEGYEKLPQSERFRDRTRHFHALAGNVDRPILSPSRNPELKLPSYASVYVFQI
                     PGTQTAAAIAYLGVERNRMLFLADLASAIRGDAQWRKVVDLADEVSTIAYTGGWLYLR
                     SSKGAPRYQVKRMPLDMPDIAKAETVIAASSSVVVNLAGARDGLYVTQRDGATLSLSR
                     LAPVGAGMPLRRESIGLPFAGNVGIDSASPRMDGVVLQLSGWTHATKPRLFDGAKLAP
                     LPFVTPGAFDAPTDIEAREVRVKGHDGVDIPMSIVLRKDTKLDGRNPTIVYGYGAYGI
                     TENPFFNPRVLAWIERGGIYAFVHVRGGGVYGDEWHQAGRKTTKPNTWRDAIAATEWL
                     IAQRYTSSDRVGIYGGSAGGILVGRAITERPELFAAAVPAVGTMDMVRAQNSANGAAN
                     IPEFGTVAKEDEFRALLAMSSYHQLKDGTKYPPVMLIHGVNDIRVDVWQSAKFASRLA
                     TANASGKPVLLRLDYESGHGQGSTRAQLQERSADLYSFMLWQFGVPEFQPSTKP"
     gene            3094..4392
                     /locus_tag="VLN25_11705"
     CDS             3094..4392
                     /locus_tag="VLN25_11705"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HSQ11256.1"
                     /translation="MLNRFRKTGLGILAVASTVTLLAACGGGSGNSGSVAAPTPGAPG
                     TPGLPGAPGNPGTPGSPPSSGGTGTLRIALTDAPACGYDHVYITVDRVRVHASSTAED
                     ADGGWSEVVVNPAQRIDLLDLTNGVLAELGQTPLPAGRYTHVRLVLKPNGTGTPANAI
                     VPTATGTEVALDTPSATQSGLKLNHGFTVQPNTMADLVLDFDACRSIVKRGNSGRYNL
                     KPVVSVVPRTLTGILGHVQTGLTGVTVSAQKNGVLLKATQPSANGQFVLAPLDPAKGP
                     YDVVFAGANLTTSVIAAVPVVTERTTTLNSSVDPVTMPTSTSGTVAGNVGPAAARASG
                     SVRALQTVGTVPAVEVAQVNVDPATGDYTQLLPTAAPRLLTYANPMVTPLNFVAQAAN
                     AAKYKLEASATGYQTQLGAEITVVPAATLGGQNFTLVVAP"
     gene            complement(4442..>5914)
                     /gene="gatB"
                     /locus_tag="VLN25_11710"
     CDS             complement(4442..>5914)
                     /gene="gatB"
                     /locus_tag="VLN25_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HSQ11257.1"
                     /translation="GASTTFGAAPNSHASVIDVALPGTLPVLNGAAVERAIRFGLAIG
                     ATIAPRSVFARKNYFYPDLPKGYQISQYEIPVVQGGSLSFVVQPKSGDAYMKTIRLTR
                     AHLEEDAGKSLHEGMGSHAALGHGMSGIDLNRAGTPLLEIVSEPDIRVTNIAGAAAEA
                     IAYAKALHSLVVWLGICDGNMQEGSFRCDANVSVRPAGSTELGTRCEIKNLNSFRFMQ
                     QAIEFEARRQIDLIEDGGTVVQETRLYDPDRDETRSMRSKEDAMDYRYFPDPDLLPLE
                     ITAQWIERVRAELPELPGAMRERFVGEYGLSEYDAQTLTDSRSKAAYYEATARQVSDK
                     KLAANWVMGEVSSALNDAGLSFDECPVSPVCLAGILNILVNGEINAKGAKILFAKRCA
                     LGDKSSDVSVPLTGQEMTVIQGELRAMVEEHGLGQISDAVAIEKIVDEVLAANLKSVE
                     EFRAGKEKAFNALVGQAMKATQGKANPQQLNEILRRRLAG"
BASE COUNT         1018 a         1950 c         1941 g         1005 t
ORIGIN      
        1 ttcagactgg cgcgcaggga atcctccgcc tgatcgaccg gtttgcagga gaacgaagca
       61 tgtccctcac acctcgacgc ggctgccgcg ctatgcggct gaccgggctg gccctattgg
      121 ccgcattcat gccgctgtcg tcggcaatgg cgcaaagcga aggcggtctg tacatcgcag
      181 gcgatgccgg cttcaagttc cggcaggcgg ctgaacgagg gctggcccag aaccccggag
      241 ggaggcgctt cttcgtgctt gcggtgccgc cgcagacgag tgcactgaag aagtcggcaa
      301 ccggaccgct cgcgcagttg cgcgaccggg tcatcgccgg caacggcgtg ctgttcgtct
      361 gccagcgcga catcgacaaa ggccgcatcg atcccgccga actcgtgccg ggcgtcgtcg
      421 cggtgcgcgg ctttccgccg cccggcagca acgcgattcc tgccggcgag cgctattttc
      481 ccgacgagga ccggtcccga ctgccggcct cgaacgaagc gttgcggcgc ctgcgcgcga
      541 cctgctcgtc ctgacaccga cctgcgcgtg gagacacctg cccgggtgat cagggtccgg
      601 cgaatcagtg caggcagtca gccgatgctc gtcagggctt ggtggacggc tggaattcgg
      661 gcacgccaaa ttgccacagc atgaagctgt acaggtcggc ggagcgctcc tgcaactgcg
      721 cacgcgtgct tccctgccca tggcctgact cgtaatcgag ccgcaatagc accggcttgc
      781 cgctggcatt ggcggttgcg agccgcgagg cgaacttggc cgattgccag acgtcgacgc
      841 gaatatcgtt gacgccgtgg atcagcatca ccggcggata cttcgtgccg tccttcagct
      901 ggtgatagct gctcatcgcc agcagggcgc gaaattcgtc ttccttggcc accgtgccga
      961 actcagggat attggcggcg ccgttggcgc tgttctgtgc gcgcaccatg tccatcgtgc
     1021 cgacggccgg caccgctgcc gcgaagagct ccggccgctc ggtgatcgcg cgacccacca
     1081 ggatgccgcc ggcactgccg ccgtagatgc cgacccgatc gctggaggtg tagcgctgcg
     1141 cgatcagcca ctcagtggca gcgatcgcat cacgccaggt gttgggcttg gtcgtcttgc
     1201 gtccggcctg gtgccactcg tcgccgtaga cgccaccgcc gcgcacgtgc acgaaggcat
     1261 agatgccgcc gcgctcgatc cacgccagca cgcgcgggtt gaagaacggg ttttcggtga
     1321 tgccgtaggc gccatagccg tacacgatcg tcggattgcg cccgtcgagc ttggtgtcct
     1381 tacgcagcac gatggacatc gggatgtcga caccgtcatg gcccttgact cggacttcgc
     1441 gtgcctcgat gtcggtgggc gcatcaaacg cccctggcgt gacgaagggc agcggcgcga
     1501 gcttggcgcc atcgaagagc cgtggcttgg tggcgtgcgt ccagccgctc agctgcagga
     1561 ccacgccgtc catgcgcgga ctggccgaat cgatcccgac gttgccggca aatggcaacc
     1621 cgatcgactc gcggcgcagt ggcatgccgg cgccgacggg tgccagtcgc gacagtgaca
     1681 acgtggctcc gtcgcgctgc gtaacgtaca ggccgtcgcg ggcaccggcc aggttgacga
     1741 ccaccgacga actggctgcg atcacggttt cggccttggc gatgtccggc atgtcgaggg
     1801 gcatgcgttt cacctggtag cgcggggcgc ctttcgaact gcgcagatac agccagccac
     1861 ccgtataagc gatagtgctg acttcgtcgg ccaggtcgac caccttgcgc cactgggcgt
     1921 cgccgcgtat cgcgctcgcc aggtcggcca ggaaaagcat ccggttgcgc tcgacaccga
     1981 gataagcgat ggcggcggcc gtctgagtac cggggatctg gaagacataa acagaggcgt
     2041 acgacggcag cttgagttcc ggattgcggc tcgggctgag gatcggacga tcgacattgc
     2101 cggccagcgc gtggaagtga cgggtgcggt cgcggaagcg ctcgctctga ggcagcttct
     2161 cgtagccctc gatcaggcgc gaatagaaga acccgctgcc atcttcgagc cacgcggtgc
     2221 tcgcatagcg gatgcggtcg atcggttcga taaccggctt gccgctcgcg acgtcgatga
     2281 catgcaaggt gccgatctcg ccgccgccga cctggatcga atacgcgagg aagcggccgt
     2341 cgcgcgatgg cgcgaagtcc atgatcgcgt ggggacggcc cccggccttc gacagggctt
     2401 ccggatcgac gatcaccgtg tcggctccgt cgatcccgtc gcgccagacc agcttgaact
     2461 gtccgtccgt gggattgcgg cgctcgaaga aaaagcgatt tccctcggtg cgcacgacgt
     2521 cggtcgcgag cccggcggca gcggcttcga tttccttcat gcgcgtgagc agacccgcgc
     2581 gcccggggat tgacaggaga acggagtccg tcgcattggc ctgcgccttc agccaggact
     2641 ggaccttggg atccttcgtg tcctcgagga agcgataggg gtccggcacg gccacgcccc
     2701 agtggttgtc gaccaccggc tgtgccggca gtggcggcgg caccgtgacg gcgacggggg
     2761 cggcgtgact cgcgcccgcg aacgcggcga cagccacgag caccatggaa agtcggcggg
     2821 agtcgacggt gcgcgcatgc atgggtgacg atcctcgtaa ggtcaaacgg cgaatcgtag
     2881 acagattggt tcgaactgag ttcgaccgtt tcggtcccca gaacacggcg gaggggactt
     2941 cgccggcgac aaaccccggt tccgggcgcc gatctcctga gcaacgtcac aggaccttcg
     3001 gattagcgcc aaagagcaac tcaagcggcc gaaaaaaggg aaaaattccc atccgtcctg
     3061 cctccgttgg acgcatcaac tgcgggaaat gccatgctga acagattcag aaagaccggt
     3121 ctcgggatcc tcgccgtggc cagcaccgtc actctgctgg cagcctgcgg cggagggagt
     3181 ggaaatagtg gaagcgtcgc ggcaccgact ccgggggcac ccggtacccc cggacttccg
     3241 ggagcgcccg gcaaccctgg cacacccggc agtccgccaa gcagtggcgg cacgggcacg
     3301 ttgcgcattg cactgaccga cgcgccggcc tgtggctacg accacgtgta catcaccgtc
     3361 gatcgcgtgc gcgtccatgc gtcttcgacc gccgaagacg ctgacggcgg ctggagcgaa
     3421 gtcgtcgtca atcccgcgca acgcatcgat ctgctggacc tgaccaacgg agtgctggcg
     3481 gaacttggcc agacgccgct gcctgccggc cggtacaccc acgtccgcct ggtactgaag
     3541 cctaacggca ccggcacgcc ggcgaatgcg atcgtgccaa cagcgaccgg gaccgaggtc
     3601 gcactcgata cgccgagtgc gacgcagagc ggcctgaagc tgaaccacgg attcaccgtg
     3661 cagcccaaca cgatggccga cctggtgctc gacttcgatg cttgccgctc gatcgtcaaa
     3721 cgtgggaaca gcggacgcta caacctcaag ccggttgtca gcgtcgtccc gcgtacgctg
     3781 acgggaatcc tgggccacgt gcagacgggg ctgaccggcg tgacggtcag cgcccagaag
     3841 aacggggtct tgctgaaggc gacgcagccg agcgcgaacg ggcagttcgt cctggcgccg
     3901 ctggacccgg ccaaggggcc ctatgacgtc gtgttcgcgg gcgccaacct gaccacctcg
     3961 gtgatcgccg cggtgccggt ggtgacggag cggaccacga cgctcaactc gagcgtggat
     4021 cctgtgacga tgccgacgtc gacctcgggc accgtcgccg gcaatgtcgg cccggcagcg
     4081 gctcgcgcaa gcggatccgt ccgcgcgctg cagacggtag gaaccgtgcc tgccgtggaa
     4141 gtcgcgcagg tgaacgtgga cccggccacc ggcgactaca cgcagctgct gccgactgct
     4201 gctccgcgtc tgctgaccta tgcgaatccg atggtcacgc cgctcaactt cgtcgcgcag
     4261 gccgcgaacg ccgccaagta caagctggag gcctccgcga ccggctacca gacccagctc
     4321 ggagccgaaa tcacggtcgt gcccgccgcg acgctcggcg gtcagaactt cacgctggtg
     4381 gtcgcgccgt aagcgcggtc ggacggcgtt ggcgggcggc ctgtcggagg tcgcccggtg
     4441 ctcagccggc caggcgccgc cgcaggatct cgttcaactg ctgcgggttg gccttgccct
     4501 gggtggcttt catcgcctgg ccgaccagcg cgttgaaggc cttctccttg ccggcgcgaa
     4561 actcctcgac ggacttcaag ttggccgcca gcacttcgtc gacgatcttc tcgatcgcga
     4621 cggcgtcgga gatttggccc aggccatgct cctctaccat cgctcggagc tcgccttgaa
     4681 tcactgtcat ctcttgcccg gtgagaggca ccgagacatc agaactcttg tcgccaaggg
     4741 cgcaacgttt cgcgaacaaa attttcgcgc ccttggcatt gatctcgccg ttaaccagga
     4801 tattcagaat gccagccagg cagacgggag acacgggaca ttcgtcaaaa ctgagaccag
     4861 cgtcgttcag tgcactcgag acttccccca tgacccaatt ggcggccagc ttcttgtcgc
     4921 tcacctgccg cgcggtcgcc tcgtagtacg cagctttcga gcgcgagtcc gtcaacgtct
     4981 gcgcgtcgta ctcgctcagg ccgtactcgc ccacgaagcg ctcgcgcatc gcgcccggca
     5041 actcgggcaa ctcggcgcgc acgcgctcga tccattgagc tgtgatttcg agcggcagca
     5101 ggtccgggtc ggggaagtag cgatagtcca tcgcgtcttc cttgctccgc atcgagcgcg
     5161 tctcgtcgcg gtcggggtca tacaggcgcg tttcctgcac cacggtaccg ccatcttcga
     5221 tcagatcgat ctggcgtcgc gcctcgaact cgatcgcctg ctgcatgaag cggaagctgt
     5281 tcaggttctt gatctcgcag cgtgtgccga gttcagtcga cccggcgggc ctcaccgaaa
     5341 cgttggcatc gcagcggaac gagccctcct gcatgttgcc gtcgcagatg ccgagccaga
     5401 ccacgagtga atgcagcgcc ttcgcatagg cgatcgcttc ggccgccgcg ccggcgatgt
     5461 tcgtcacgcg gatgtccggt tcggaaacaa tctccagcag tggcgtgccg gcgcggttga
     5521 gatcgatgcc ggacatgccg tggccgagcg cggcgtgcga gcccatgcct tcgtgcagcg
     5581 acttgcccgc atcctcctcg agatgcgcgc gcgtcaaccg aatcgtcttc atgtaggcgt
     5641 cgccgctttt gggctgcaca acgaaactga ggctgccgcc ctgcacgacc gggatctcgt
     5701 actggctgat ctggtagccc ttcggcagat cgggatagaa gtagttcttt cgtgcgaaca
     5761 cgctgcgcgg tgcaatcgtc gcgccgatcg ccaggccgaa gcggatcgcg cgctcgacag
     5821 cggcgccatt gagcaccggc aacgtgccgg gcagcgccac gtcgatcacg ctcgcgtgcg
     5881 aattcggcgc agccccgaat gtcgtggagg cacc
//
LOCUS       DATIRT010000513         4344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_878193, whole genome shotgun sequence.
ACCESSION   DATIRT010000513 DATIRT010000000
VERSION     DATIRT010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878193"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(259..477)
                     /locus_tag="VLN25_11715"
     CDS             complement(259..477)
                     /locus_tag="VLN25_11715"
                     /inference="COORDINATES: protein motif:HMM:NF025005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HSQ11258.1"
                     /translation="MAIERKPIRRGGIKSAGYDRNLRVLEIEFDNGQVIQHTAVGEEI
                     ARRFLASSSPASVYKDTIQEEFTVKRIR"
     gene            complement(481..2007)
                     /gene="ubiD"
                     /locus_tag="VLN25_11720"
     CDS             complement(481..2007)
                     /gene="ubiD"
                     /locus_tag="VLN25_11720"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HSQ11259.1"
                     /translation="MKYRDLRDFLDRLEATQQLRRIRTPVSTRLEMTEISDRTLRAGG
                     PALLFERPEGHEIPVLTNLFGTPDRVALAMGADNVGALREVGELLASLKEPEAPRGLR
                     DALGKVALLKSALWDMAPKEIRSAPCQEVRFEGPDVDLARLPIQTCWPGDAGPLITWG
                     LVVTRGPHKPRQNLGIYRQQVIGRNKLIMRWLAHRGGALDFRDHALAFPGQPFPIAVA
                     LGADPATILGAVTPVPDNLSEYQFAGLLRGGRTETVKCIGSDLRVPATAEIVLEGHLQ
                     PQPVPPAVPSDAPPRPASDYELALEGPFGEHTGYYNERDWFPVFTVDRITTRRDPIYH
                     STYTGKPPDEPAVLGVALNEVFVPILQRTFPEITDFYLPPEGCSYRMAVVSMRKQYAG
                     HAKRVMFGVWSFLRQFMYTKFIVVVDDDVNPRDWKEVIWAITTRVDPVRDTVLVDNTP
                     IDYLDFASPVSGLGSKMGIDATNKWSGETAREWGRPITMDAAVKSRVDSIWKELGLEE
                     "
     gene            2467..3354
                     /locus_tag="VLN25_11725"
     CDS             2467..3354
                     /locus_tag="VLN25_11725"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010396183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HSQ11260.1"
                     /translation="MSAATNSISLAPATSVAWAEVVLDVRDGALATARALVTLAGSAA
                     LLVGAIVVANHDVRSALLSALPFPVGVVSAPMEELAEIASEPATAPAAVPAAADPRQK
                     HVVQYLARRYRVAEEATRMLVSTAFKIGHENKLDPLLILSVVAIESSLNPFAESSMGA
                     QGLMQVMTRVHAERFEAHGGQIAALDPVANMKVGSAILSDLISRGGSVERGLQLYVGA
                     GNLPDDGGYGARVLSERSRLALAASGKVEAAISAGRTTVVSEQKPATADATLPAQPEA
                     RPAVESPLPVEEAAPARSA"
     gene            3895..>4344
                     /locus_tag="VLN25_11730"
     CDS             3895..>4344
                     /locus_tag="VLN25_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HSQ11261.1"
                     /translation="MPPTEHDDLRRAALEYHEKPTPGKIAISPTKQLVNQRDLSLAYS
                     PGVAAASEAIHADPAAAFRYTARGNLVGVVTNGTAVLGLGNIGPLAAKPVMEGKAVLF
                     KKFAGIDVFDIEVNELDPDRLIEVIAALEPTFGGINLEDIKAPECFYV"
BASE COUNT          786 a         1339 c         1406 g          813 t
ORIGIN      
        1 gcacgccaga ccgagctgct tgccgcggaa ccgggtttga ctttccctcc tcgcctggcg
       61 aggagggcgc agggaggagt gcctcgttcc ctctcgctcg gcgagagggt cggggtgagt
      121 gccgtgcact tgaaagtcgg tggagcgcgc ggccgctccg gagcgcagtc gactaaatga
      181 tcagttccgc tccgagtgca cgtgcgaccg cgcagcggtc gcgtacacga ggagcgcaca
      241 actcggccgc gtacacggct agcgaattcg ctttaccgtg aactcttcct ggatggtgtc
      301 cttgtagacg cttgcgggcg aagaactggc caggaagcga cgcgcgatct cctcgccgac
      361 ggcggtgtgc tggatcacct gcccgttgtc gaactcgatt tcgagtacgc gcaggttgcg
      421 gtcgtagccg gcggacttga tgccgccgcg ccggatcggt ttgcgttcga tcgccatgct
      481 tcactcctca agtccgagtt ccttccagat cgaatcgacg cgggacttga ccgccgcatc
      541 catcgtgatc ggtcggcccc attcgcgcgc cgtctctccg ctccacttgt tggtcgcatc
      601 gatgcccatc ttgctgccga gccccgacac cggcgaggcg aaatcgagat agtcgatcgg
      661 cgtgttgtcg acgagcaccg tgtcgcgcac tggatcgacg cgcgtcgtga tcgcccagat
      721 cacttccttc cagtcgcgcg ggttcacatc gtcgtcgacc acgacgatga actttgtgta
      781 catgaactgc cgcaggaagc tccagacacc gaacatgacc cgcttggcat gccccgcgta
      841 ctgcttgcgc atcgaaacga cggccatgcg atagctgcag ccttccgggg gcaggtagaa
      901 gtccgtgatc tccggaaagg ttcgctgcag gatgggtacg aagacttcgt taagtgcgac
      961 gccgaggacg gccggctcgt ccggcggctt gccggtgtag gtcgagtggt agatcggatc
     1021 gcgtcgcgtc gtgatgcggt cgacggtgaa caccgggaac cagtcccgtt cgttgtagta
     1081 gccggtgtgt tcgccgaacg gaccttcgag tgccagctca tagtccgagg cgggccgggg
     1141 cggtgcgtcg gacggtaccg ccggtggcac aggctgaggc tggaggtgac cctcgaggac
     1201 gatctccgca gttgccggga cccgcaggtc gctgccgatg cacttcaccg tctcggtgcg
     1261 gccgccgcga agcaggccgg cgaactggta ctcggaaagg ttgtccggga caggcgtgac
     1321 ggcacccagg atggtggccg gatccgcacc gagggcgacg gcgatcggaa agggctggcc
     1381 cggaaacgcg agcgcgtggt cgcgaaaatc gagcgcgcca ccacgatggg ccagccagcg
     1441 catgatcagc ttgttgcgac cgatcacctg ctgccggtag atacccaggt tctggcgggg
     1501 cttgtggggg ccgcgcgtaa ccacgaggcc ccaggtgatc agcgggccgg catcgccggg
     1561 ccagcaggtc tggatcggca ggcgtgccag gtcaacgtcc ggaccttcga agcggacctc
     1621 ctgacagggg gccgagcgaa tctccttggg agccatgtcc cacagcgcgc tctttagtag
     1681 agccaccttg cccagcgcat cccgcagacc ccgtggcgcc tcgggctcct tgaggctcgc
     1741 cagcagctca cccacctcgc gcagggcgcc gacgttgtca gctcccatgg caagcgccac
     1801 ccggtcgggg gtcccgaaga ggttggtcag caccggaatc tcgtgacctt cagggcgctc
     1861 aaacagtagc gccggcccgc cagcccgcag agttcggtca ctgatttcgg tcatctcgag
     1921 gcgggtcgag accggcgtgc ggatgcgacg cagttgctga gtggcctcca aacggtccag
     1981 gaagtccctc aagtcgcggt atttcatcgg aaatttgttg caaagcagaa aataattgaa
     2041 ggctctaaat aagcacttac taacattgaa aaagtctatt taagtctatg aaaacaagct
     2101 ggaaattgca aagcccttcg acctcacgcg ggttcgatac tgctcccgcc ggtcggaaat
     2161 gcggaatttt cgggttgacc catcgacccc agaccctaat attccgttca gccattcggc
     2221 cgactccgct ctcccgcctc ggcgaagcta caaaaccgga gtctagggac ttaacagtcc
     2281 tgctgtttgt acttttatgt gatcgggaca cgtcccgttc gaaccggtgc tggtagcggc
     2341 ccttgggccg aagccggcat cccgagaatc cgaactggct cgtgggccat cgggttcaac
     2401 gtaccaacct gtgagtggcg accggaggtc tcgtgacccg tggccgttca gcataaggag
     2461 gcacgcatgt ctgcagccac gaattcgatc tccctggctc ccgcgacttc ggttgcgtgg
     2521 gctgaagttg tcctcgacgt acgcgacgga gcactcgcaa ccgcgcgtgc cctggtgacg
     2581 ttggccggta gcgccgcgct gctcgtcggt gccatcgtgg ttgccaatca cgacgtacgg
     2641 tccgcgcttc tttcggcact tccgttcccc gtcggggtag tttcggcgcc gatggaggaa
     2701 ctggccgaga tcgcgtcgga accggcgacg gctccggccg ctgtaccggc ggccgccgat
     2761 ccgcgccaga agcacgtcgt gcagtacctg gcgcggcgct atcgggtcgc agaagaggcg
     2821 acgcgcatgc tggtgtcgac cgccttcaag atcggtcacg agaacaagct ggacccgctg
     2881 ctgatcctgt ccgtggtggc gatcgagtcc agtctgaatc ccttcgccga gagttcaatg
     2941 ggggcccagg gacttatgca ggtcatgacc cgcgtccacg ccgaacgctt cgaggcgcat
     3001 ggcggacaga tcgcagcgct ggatccggtg gccaacatga aagtcggttc cgcgatcctg
     3061 agcgacctca tctcgcgcgg cggctccgtc gagcgcgggt tgcagttgta tgtcggggcg
     3121 ggcaacctgc cggacgatgg cggctacggt gcacgagttc tgagtgaacg gtcgcggctg
     3181 gcgctcgcag ccagcggcaa agtcgaggcg gcaatttctg ccgggcgcac aaccgtcgtc
     3241 tcggagcaga aaccggcaac ggctgatgca actctcccgg cgcagccgga ggcacgtcca
     3301 gcggtcgagt ctccgctgcc ggttgaagaa gccgcgccgg cccgttcagc ttgatgtctc
     3361 gttgaccggg ggccctgctg aacgcagccg ggtgtcgggt ctgaacttgc tgtcagtcga
     3421 cagcgccttc cacttacttt tgctatgcat gccgagagcc gattcgcctc gcacgagtcg
     3481 gtgcggcgtt gcccaaagca cgtgctaaat gttagtgcgt gctctctaat ttgtcgtgga
     3541 caaaaaaaga gcctgtcacc tggacgcgcc cacattcggt aagaggaggg aggaggagaa
     3601 gcaccgagta gggttgccgc gcccaggcca tacgacaggc tctgagaaag cttgagcgaa
     3661 gtctactgtt gctacggcgg ttgtcaagtt tgtctaggtc aaaatagctt tgccggcgac
     3721 cggagtatta gggtgggccg atcttggtgc cagtctgccg tctctcgaat ctgcatcgga
     3781 aacatcgcgg cgggcgttag tgggtcatgg cgatgggctt ggcgtgtcgg accatgcgca
     3841 ggatcaggtc ggcacggata atggccgttc tgcgcatcag gcccatcaaa ccacatgccc
     3901 ccgaccgaac acgatgacct gcgccgcgcg gcgctcgagt accacgagaa gccgacgccg
     3961 ggaaagatcg cgatctctcc gacaaagcag ctcgtcaatc agcgagatct ttcgctggcc
     4021 tattccccgg gtgttgccgc ggcgagcgag gcgatccatg cggaccccgc ggccgcattt
     4081 cgctataccg cgcgcgggaa cctggtcggc gtcgtgacca acggcaccgc ggtgctcggc
     4141 ctcggcaaca tcggtccgct ggccgccaag cccgtcatgg aaggcaaggc cgtgctgttc
     4201 aaaaagtttg ccggcatcga tgtcttcgac atcgaagtca atgaactcga cccggaccgg
     4261 ctcatcgaag tcatcgcggc gctggaaccg acgtttggtg gcatcaacct cgaggacatc
     4321 aaggccccgg agtgctttta cgtc
//
LOCUS       DATIRT010000514         2844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1075513, whole genome shotgun sequence.
ACCESSION   DATIRT010000514 DATIRT010000000
VERSION     DATIRT010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075513"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VLN25_11735"
     CDS             complement(<1..185)
                     /locus_tag="VLN25_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11262.1"
                     /translation="MAGIREIPDTPACEPDDANAAERSAFERDAPGESDEFARALNGM
                     EAEASDDDYEPDNVQRYL"
     gene            complement(192..968)
                     /locus_tag="VLN25_11740"
     CDS             complement(192..968)
                     /locus_tag="VLN25_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HSQ11263.1"
                     /translation="MVPPAQRVAKEGIYTVQRGDTLYSIATSFGVDVRELARMNNLGE
                     SASLSVGQSLRVTAPPTSAATVTPVAPVGSAEVRPLPGADASPPVATAPGATLPPAAP
                     SLPAMPSTPSPQPPPVVPGAATVPPETVKPAPLESSVQWLWPAPGKVIENFDETRNKG
                     LDIAGNEGDPVQAAGDGEVVYVGSALRGYGNLVIVKHNDEFISAYAHNRQILVKQGQA
                     VKRGQRIAEFGRSDADRVKLHFEIRRHGKPVDPLRYLPVR"
     gene            complement(1114..1806)
                     /locus_tag="VLN25_11745"
     CDS             complement(1114..1806)
                     /locus_tag="VLN25_11745"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HSQ11264.1"
                     /translation="MRRPASPASEPSQTHGGLASAKVRDRMVDRVRELGVRDSRVLDA
                     MRAVPRHLFVDEALASRAYEDTPLPIGHGQTISQPYIVARTVELALEGIADPRHARVL
                     EIGTGCGYAAAVLAQLVGEVVSIERVRALHERARDNLRALRLPNLRLVFGDGSRGVEQ
                     GAPYDAIVAAAAGDAIPSAWVEQLKPGGRIVAPVGSGEQQLVVAQKDESGKLIRSGKE
                     AVRFVPLRGGVA"
     gene            complement(1808..2554)
                     /gene="surE"
                     /locus_tag="VLN25_11750"
     CDS             complement(1808..2554)
                     /gene="surE"
                     /locus_tag="VLN25_11750"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HSQ11265.1"
                     /translation="MRILVSNDDGYLAPGLAALAAAMRPFGTVTVVAPEQNSSGASNS
                     LTLSRPLTVSTASNGVTFINGTPSDCVHIALTGLLDERPDLIVSGINNGQNMGDDTIY
                     SGTVAAAMEGYLFGIPAMAFSLVDKGYAHLESAAAMAAEVVRRHIQQPLPGAFLLNVN
                     LPNRPVADIRGIVATRLGKRHPSEPVVRAVNPRGEPIYWIGPAGGAKEGGPGTDFHAT
                     SAGYVSVTPLQIDLTHDDQLDGVRAWLERA"
BASE COUNT          485 a          964 c          913 g          482 t
ORIGIN      
        1 aggtagcgct gcacgttgtc gggctcatag tcgtcgtcgg aagcttccgc ttccataccg
       61 ttcagcgcgc gagcaaattc atcgctttca ccgggcgcgt ctcgctcgaa agcgctgcgc
      121 tccgcggcgt ttgcgtcgtc cggctcgcag gccggcgtgt ctggaatctc gcggataccg
      181 gccatgcctg ctcagcgcac cggcaggtag cgcaacggat cgaccggctt gccgtgacgc
      241 cgtatctcga aatgaagctt gacccggtct gcatcgctac ggccaaactc ggcaattcgc
      301 tggccgcgct tcaccgcctg cccctgcttg acgaggatct ggcgattgtg cgcgtacgca
      361 gaaatgaact cgtcattgtg cttgacgatg accaggttgc cgtagccgcg caacgcgctg
      421 ccgacgtaga cgacttcccc atcaccggcc gcctggacag ggtcgccctc attgccggcg
      481 atatcgaggc ccttgttgcg cgtctcgtcg aagttctcga tcacctttcc gggcgccggc
      541 cacaaccact gcactgagct ctccagcggc gccggcttga ctgtctccgg cggtacggtg
      601 gcggcgccgg gcaccacggg cgggggctgg ggcgaaggcg tcgacggcat cgcaggcaag
      661 ctcggcgcgg ccgggggcaa cgtggcgccg ggcgctgtcg cgaccggtgg cgatgcatcg
      721 gctcccggca ggggccgaac ctccgcagat ccgaccggcg ccaccggggt gacagtcgcc
      781 gcgctggtcg gcggtgccgt gactcgcaac gactggccga ccgaaagcga cgcggattca
      841 cccaggttgt tcatgcgtgc aagttcacgg acgtcgacac cgaacgacgt cgcgatgctg
      901 taaagggtgt ctccgcgctg taccgtgtag atgccttcct tggcgacgcg ctgagccggc
      961 ggcaccattg gtccggccgc cgcatcgggg cgaacattcg tcggtccgga gcgatcaacc
     1021 accggagccg gacgctgcgt cccacacgct gccagcatca aaacgacgcc gcaaaagaca
     1081 cccccaccga cccaccgcct catccgctgc ctcctaggcc acaccaccac gcaagggcac
     1141 gaagcggaca gcttccttgc cgctgcgaat caacttcccg ctttcgtcct tctgcgccac
     1201 gacaagctgc tgctcgcccg agccgaccgg cgcgacgatg cgtcctcccg gcttcagttg
     1261 ctcgacccag gcggacggta tcgcatcccc cgcggccgca gcgacgatcg catcgtaggg
     1321 tgcgccctgc tccacaccgc gtgacccatc accgaagacc agacgaaggt tcggcagacg
     1381 cagcgcacga aggttgtcac gcgcgcgctc atgcagcgcg cggacgcgct cgatgctgac
     1441 gacctccccg accaactgag ccagcaccgc ggcggcatac ccgcagccgg tgccgatctc
     1501 gagcacacgc gcgtgccgcg gatctgcaat gccttcaagc gccagttcga ccgtgcgcgc
     1561 gacgatatag ggctgtgaaa tcgtctggcc gtgtccaatt ggcagcggcg tgtcttcata
     1621 ggcgcggctc gcgagtgctt cgtcgacaaa caggtgcctc ggaacggccc gcatcgcatc
     1681 cagcacccgg ctatcgcgca cgccgagttc ccgcacgcga tcgaccatgc gatcgcgtac
     1741 ctttgccgat gccaggccgc cgtgcgtctg tgatggctcg ctcgccggcg atgcgggccg
     1801 acgcatttca ggcccgctcc agccaggcac gcacgccgtc cagttgatcg tcgtgagtca
     1861 ggtcgatttg taacggtgtc accgacacgt agccggccga ggtcgcatgg aagtcggtac
     1921 cgggtccgcc ttccttggca cctccggccg gaccgatcca gtaaatcggt tcaccacggg
     1981 gattgacggc gcgcaccacc ggttccgacg ggtggcgttt gcctagtcgg gtggcgacga
     2041 ttccgcggat atcagccacc ggacgattcg gcaggttcac gttcagcaga aaggcgccgg
     2101 gcaagggctg ctgaatgtga cgacgcacga cttcagcggc catcgcggct gcagattcca
     2161 ggtgcgcgta acccttgtcc accagcgaga aggccatcgc cgggatcccg aagaggtagc
     2221 cctccatcgc ggctgcaacc gtgcccgagt agatcgtgtc gtcgcccatg ttctgcccgt
     2281 tgttgatgcc ggaaacgatc aggtccgggc gttcatcgag caaccccgtc agggcgatat
     2341 gtacgcagtc gctgggcgtg ccattgatga aggtaactcc attggacgcc gtgctgaccg
     2401 tgaggggtcg tgacagggtc agggagttgc tggcaccgct gctgttctgc tcgggcgcga
     2461 caacagtgac ggtaccgaac ggacgcatcg ccgcagcgag cgcagcgagt ccgggagcca
     2521 gatagccatc gtcgttggaa accaggatgc gcatgggaac ctatctcaga gtccccggtg
     2581 gtcgcggcgg gccgcattgg gcgcagggct aggcgcggcg aggggaccat tgctgatgca
     2641 aggacccgag ccgcaacaac gccatgcgtc caatgcgacc cagccccgga gggttgtcac
     2701 gataaccggg cgtctgcggc gttggctggc tcggccgtag gctcggctac tgtccttcgc
     2761 cagccgcctt gcatccgccc ggttctcgtg gcaacgcgat ccatcggcga ctctgagata
     2821 ggttccgaaa aatagccggt cgcg
//
LOCUS       DATIRT010000515         1871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1468522, whole genome shotgun sequence.
ACCESSION   DATIRT010000515 DATIRT010000000
VERSION     DATIRT010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468522"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VLN25_11755"
     CDS             <1..373
                     /locus_tag="VLN25_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HSQ11266.1"
                     /translation="HLELVARELYSEAFFGGKLSDSAKERVRKQLEKNIAAFKRLARF
                     APYVAGERFTLADCSAYASLPVVAMATKAIYGEDLLAAAGIDWKGYSKLIGERPSAVK
                     VSADRKADQERRARAAAAAKT"
     gene            440..1456
                     /locus_tag="VLN25_11760"
     CDS             440..1456
                     /locus_tag="VLN25_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HSQ11267.1"
                     /translation="MMQVSFASLEYAGKKRQTRREKFLGEMDRVVPWDRLVALIEPHY
                     PRAGRVGRQPIGVPRMLRMYFLQQWYSLSDEGLEDAIYDSQAMRDFIGIDLSREQVPD
                     ATTLLKFRRKLEEHQLTKAIFEQVNADLQARGLLMREGTLVDATIIAAPPSTKNKDKA
                     RDPEMHQTKKGNQWYFGMKAHIGADAESGLVHSVGASAANESDVAHTHGMLHGQERRA
                     HLDAGYIGVDKRDEIVAAQKQGRIRADIEWQVATKRGTIKAMAEGPYKELTVLVERKK
                     AQIRALVEHPFHVLKNLFRYKKVSYRGVHKNQVRLHAQFALVNLLLAKRALLDGQVRG
                     ISAS"
     gene            1657..>1871
                     /locus_tag="VLN25_11765"
     CDS             1657..>1871
                     /locus_tag="VLN25_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11268.1"
                     /translation="MSELKFRPAVSSDADRLEVIRALAFAPVFASFRVLLGDTIYERA
                     QRPRDEAHRELPSSLVCGVPGWAPWVA"
BASE COUNT          388 a          572 c          604 g          307 t
ORIGIN      
        1 gcacctggaa ctggtggcgc gcgagctgta ttccgaggct ttcttcggcg gcaagctctc
       61 cgattcggcc aaggagcgcg tgcgcaagca gctcgagaag aacatcgcgg ccttcaagcg
      121 cctggccagg ttcgcgccct acgtggccgg tgagcggttc acgctggccg actgctcggc
      181 ctacgcgtcg ctgccggtgg tggcaatggc cacgaaggcg atctacggcg aagacctgct
      241 cgccgccgcc ggcatcgatt ggaagggcta cagcaagctg atcggcgagc ggccgagcgc
      301 ggtgaaggtg tcggccgacc gcaaggccga ccaggagcgt cgcgccaggg ccgctgccgc
      361 ggccaagacc tagggaaatg ctgatcaatt cgggtcaggc tcgattgtcc tgctccgacg
      421 ctgcacagat gatctcgtca tgatgcaagt aagctttgcc agcctggagt acgcaggcaa
      481 gaagcgccag acgcggcgcg agaagttcct cggcgagatg gatcgcgtcg taccctggga
      541 ccgtcttgtc gcgttgatcg agccccacta cccgcgtgcc ggccgagttg gacggcagcc
      601 aatcggtgtg ccccgcatgc tgcgcatgta cttcctgcag cagtggtaca gcctgagcga
      661 cgaagggctc gaagatgcca tctacgacag ccaggccatg cgcgatttca tcggcatcga
      721 cctctcgcgc gagcaggtac ccgacgcgac gacgctgctg aagttccgcc gcaagctcga
      781 ggagcaccaa ctgaccaagg cgatcttcga gcaggttaac gccgatctgc aggcacgtgg
      841 cctgctcatg cgcgagggca ccctggtgga tgccaccatc atcgccgcgc caccgtcgac
      901 caagaacaag gacaaggcgc gcgacccgga gatgcaccag accaagaagg gcaaccagtg
      961 gtacttcggc atgaaggctc acatcggagc cgatgccgag tcgggcctgg tgcacagcgt
     1021 gggcgccagc gcagccaacg agtccgacgt ggctcatacg cacggcatgc tgcatggaca
     1081 ggagaggcga gcgcaccttg acgccggcta catcggcgtg gacaagcgag acgagatcgt
     1141 ggcagcgcag aagcaaggtc gcatccgtgc cgacatcgag tggcaagtgg ccaccaagcg
     1201 cggcacgatc aaggcgatgg ccgaagggcc gtacaaggaa ctgacagtcc tggtcgaacg
     1261 caagaaggcg cagatccggg cgctggtcga gcacccattt cacgtgctca agaacctgtt
     1321 ccgctacaag aaggtcagct atcgcggggt gcacaagaac caggttcggc tgcatgcgca
     1381 gttcgcgctg gtgaacctgt tgctggccaa gcgggcacta ctggatgggc aagtcagggg
     1441 gatcagtgcg tcctgaaggg caatcagccc gggaagaccg cttcggcggt caagaaacca
     1501 tcggaatcga ccgctgcaag cacagaaact ccgcatttga aatccaagcg tcgtttaatc
     1561 aagatcaact gctggataac gcctttattc agcggttcct tagcggacgc ggatgctcga
     1621 agctggtcca cccgcagaac agagacggga agccgaatga gtgaactgaa attccggccg
     1681 gcagtttcat ccgatgccga tcgcctggag gtgatccgcg ctcttgcatt cgcgccggtc
     1741 ttcgcgtcct ttcgtgtcct gctcggagat accatttacg agcgggccca acgaccacgg
     1801 gacgaggctc accgcgaact cccttcctct ctggtctgcg gcgttccggg atgggcccct
     1861 tgggttgccg a
//
LOCUS       DATIRT010000516        11608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_485335, whole genome shotgun sequence.
ACCESSION   DATIRT010000516 DATIRT010000000
VERSION     DATIRT010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11608
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485335"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="VLN25_11770"
     CDS             complement(<1..1136)
                     /locus_tag="VLN25_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11269.1"
                     /translation="MQDVALPVKAPQEVASLSATAASLDFDLGDLDSDAERPAEGADV
                     RSREAATDEEDSVQSAEAAALEMQFELPELVPPDEDVVLEAIPVESSGIDDEDVRRIG
                     PLEISHGLYSVFLNEADECVRVLAHDINEWRYEPSRPVSAQVVRRAHSLSGISKTVGL
                     LPVIAISDPLDDLMHTLSMLSGPRHYALTAPQFDTLERVIEHMRGMLHQFAAGVYPDE
                     APLDAGALQDVVAIVRAHSALHDELTPVVVHPSATREVEAPQWANITLIEPTPSEAGD
                     LAAAQPAPLVDAVSTADTVAVSVVEAAAPVVVTEAIPVYVPTPAANVPDVPAKASAAS
                     PIEVATVAETEAEVAAATRVRDELDADLLQVFLAEAADLLPSIGT"
     gene            complement(1188..3701)
                     /locus_tag="VLN25_11775"
     CDS             complement(1188..3701)
                     /locus_tag="VLN25_11775"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HSQ11270.1"
                     /translation="MAERHDPNQLVGAAPDIAPLAWVIDEIRNSLTEAVNGLKAFLAN
                     KQDVDSLRNARNQVHQANGALQLLDLRGVALVTDAVEQLTRRFEAEPKDCLPAAVRSV
                     ETSLTAVVAYLEALLSGRPNQPIRLFPYYRDVLQLNRANRVHPADLIFPDLSRRPAFH
                     QIEARNYPPDQLRDRRARFELGLLGFLRNSDDEGARKRMREALADLEHLPQRGLARSF
                     WWVVRGLLEGLDSNVVPVDVDLKRVLARLNLQLRRMIEGGGAVAERLMIDALYYVGRA
                     QGGPARVAEIKRLYGLDALIPADFERASLTAVDADALRSIKEALASAKQLWGQIVAGS
                     PETAKFEQEITLARTSASRLSARAVGTTVEIIGKVTKDFAKLPADVRERLGLEVASAL
                     LFTDIGVDDLPLTDDEYEGRAQLVIERLLKARQGQPLPEAGPWMSELARRAQDRLTMG
                     TVVAETQSTLREIEQRLDRFFRNPLERSDLPATTAMFDQVCGVLSLLGYEDPVAALRN
                     VQESIGRFADAKIAPEPDEFGRIAQNLGAVGFFVESLGQDTERPRGMFHYDPSTGVFT
                     AELGQLPADASVFAPADEEYQAAPRTIDMRVPEPRRTDNVETAARKTLEAAHHLAERL
                     THVAGDARAIGELDRLLPMLSNEADLLDNEQLKAKAARALQLLTQLKDLPVREDAVEL
                     EQLLAPAKAPEAPPPTAPLPSTQAAADSELREIFVEEAHEVLDSIVENLNDLRTRRDD
                     KATLTTVRRAFHTLKGSSRMVGFKHIGEGAWGVEQCFNLWLAQERPATDDLIDLANGA
                     QRVIRAWVETIDRDPFANIDPTPLVVASQRVREGGMFEI"
     gene            complement(3834..6062)
                     /locus_tag="VLN25_11780"
     CDS             complement(3834..6062)
                     /locus_tag="VLN25_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HSQ11271.1"
                     /translation="MALEEVGSLFKRKSRERKSRDGAEPVRGAASPPPKTVMIDRGNE
                     GMTMRLVDRLLAVKLPLIGDKPVNTQVQVLLILLGFCFVVLVAVLFFDNRLAANGAMQ
                     TEIVGDTLMHTQRLAKAAPNAVEGVREAFVELRESRDAIAANLDALANGDTLRNLSAT
                     ASDMQPALTKLIDRWRTSEAAASVIIANEKALLAFGDVIRKINDASPRLQQLTEEITA
                     LKLQSGAPAREIATAAQLVTLTQRLGKSANSLVAGSSANAEVALTLGRDINQFRDLTQ
                     ALLVGSDTLRLAASTDSETRVRLQELTRVYGEFQASIAGAIGNLQAIVQGKEAELAIL
                     RDSEQLRQQVTELGGLYTKEQATRKVNLFIMALFAIGLLLCAFAIVKVLLEDTRKRAA
                     EAEIQRMQAERLEQEAKRTNEQNQAAILRLMNELQEVADGNLTVQATVSEDITGAIAD
                     SVNYTVEELRGLVGRINNTAEQVNTASTAARDIATRLLTASEAQSKEIKDTGDKVLKM
                     ASQINDVSKSAGESAKVARASLDAAERGQRAVFNQIAGMNEIREQIQETAKRIKRLGE
                     SSQEIGEIVELITDITEQTNVLALNAAIQAASAGDAGRGFSVVAEEVQRLADRSAEAA
                     KQIGALVRTIQTDTQDAVAAMEKSTQGVVEGTKLSDAAGNALGDIGRVSRQLAELIER
                     IASTTNDQASSAGGVAQSIERILAVTEQTSHGTRQAAQSIGQLATLARELKASVARFR
                     VG"
     gene            complement(6172..6708)
                     /locus_tag="VLN25_11785"
     CDS             complement(6172..6708)
                     /locus_tag="VLN25_11785"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HSQ11272.1"
                     /translation="MATQRDVSPRQLVKLREFSTQLAQRLKEAPNLPTEPTRLAVRMG
                     VENYLVEMGLAAEIVSLPETARVPWTKPWYRGLANVRGRLVGVIDLQHLMGREPLAAE
                     QGQQLLVLGEGLGVAAGILITRAFGIRNLKDLESVDTTVSASGWERSRYRDLEGLMLT
                     ELDLREVAAAPAFTAINA"
     gene            complement(6720..7085)
                     /locus_tag="VLN25_11790"
     CDS             complement(6720..7085)
                     /locus_tag="VLN25_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSQ11273.1"
                     /translation="MPIKKVLIVDDSPTERFFLNELLTKQGYAVSTAENAEEAMMKVK
                     AVMPDLILMDVVMPGQNGFQLTRALSKDPVTQDIPIIMCTSKNQQTDRIWGLRQGARD
                     YITKPVKPEELFEKITALG"
     gene            complement(7109..7504)
                     /locus_tag="VLN25_11795"
     CDS             complement(7109..7504)
                     /locus_tag="VLN25_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSQ11274.1"
                     /translation="MAAQDFSDIRGKKVMVIDDSNTIRRSAEIFLSQAGCQVILAQDG
                     FEALSKINDHQPNIIFCDILMPRLDGYQTCALIKRSPRFHKTPVVMLSSKDGLFDRAR
                     GRMVGSSEYLTKPFTKDSLLSAVKQHSMN"
     gene            complement(7662..7844)
                     /locus_tag="VLN25_11800"
     CDS             complement(7662..7844)
                     /locus_tag="VLN25_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HSQ11275.1"
                     /translation="MTTNVGTKTWMCLICGWIYDEAAGVPESGIAAGTRLEEIPPNWT
                     CPECGARKEDFEMVEI"
     gene            8010..8864
                     /locus_tag="VLN25_11805"
     CDS             8010..8864
                     /locus_tag="VLN25_11805"
                     /inference="COORDINATES: protein motif:HMM:NF020132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HSQ11276.1"
                     /translation="MPTLPVVLSFGVSDATGAAGMQADQLAIASMGCHPASVITAIAT
                     AEDQFAHDWLALDEELVEGQAQAVLQNMAVAAFKVGAIGSVDQMQSIASILADYDTVP
                     VVLDPVLDRTDDDDASGELAAALRELLMPQTTIVTVNLAQARRLVSLADDDEDRSHDL
                     LAAACAREIIGWGAEFVLMTDAEPGSALVINALYDESGMVRSDSLPRVDAVSTRLHGA
                     GDTLSAALAGLLAQGLDVPEAAQEASQYTAAALVHAFSSGIGVAIPDRLFWAGDDDDD
                     DDRSSDVN"
     gene            8854..9540
                     /gene="thiE"
                     /locus_tag="VLN25_11810"
     CDS             8854..9540
                     /gene="thiE"
                     /locus_tag="VLN25_11810"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HSQ11277.1"
                     /translation="MSTDRRDRADRVAGVYLLTPDTGPGDFEAMAARFEAAVAAGVSI
                     VQYRNKAASAEDRRAQARVLQAIARKHNALFMVNDDIDLAIQLEADGVHLGRNDGDIA
                     VARQRLPRQLLGVSCYNDLENARRAVDAGADLLAFGSVFASVTKPAAVRASLGLLRDA
                     RERFPQQRVIAIGGIDATNIGSVSAAGAHAAALISAVFDAGDPGQAVHSLQKEFREGL
                     IQHDTQRTAF"
     gene            9515..10828
                     /gene="hemL"
                     /locus_tag="VLN25_11815"
     CDS             9515..10828
                     /gene="hemL"
                     /locus_tag="VLN25_11815"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HSQ11278.1"
                     /translation="MTRNEQLFERAQRSIPGGVNSPVRAFRSVGGTPRFIERAEGPYF
                     WDADGKRYIDYIGSWGPMILGQVHPAVVKAVQEAAARGLSYGAPTEAEVVMAEELLKL
                     VPSMQQLRLVSSGTEAAMSAIRLARGAAGDGKGRRKIIKFEGCYHGHADSLLVKAGSG
                     LLTFGNPSSAGVPAEITQHTLVLDYNEPQQLEDAFRQHGDDIACVIVEPIAGNMNLVR
                     STAEFLRTMRELTAKHGAILVFDEVMSGFRVALGGAQALYGIDPDVTVLGKVIGGGMP
                     VAAFGGKREIMRHLAPLGGVYQAGTLSGNPVAVACGLATLREIQRPGFYKTLSQRTRD
                     LVTGLTAAARSAAPGLAFSGDSVGGMFGLYFAAEPPATYAQVMAGNKERFNRFFHAML
                     ERGVYLAPSAFEAGFVSMAHDAAVIDATRTAAHDAFAAIAELAAG"
     gene            10878..>11608
                     /locus_tag="VLN25_11820"
     CDS             10878..>11608
                     /locus_tag="VLN25_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HSQ11279.1"
                     /translation="MHTRRLLIPFLTAALLSLLTATAAAQSLPRFGAPDPHVKAELVA
                     ATDAVVPGQPLQVGLRLKHESEWHTYWQVPGDSGLPTRIEWQLPAGMTAGPIEWPHPH
                     RLPVGPLMNFGYEGETLLLTTVQVGDVKAGTPVTLNAKVEWLECKDVCIPGSADLSLT
                     LPVKASSAPSSHAGLFTATRRLVPVTVAGLAARGTIESNRIRLAFDLPSGKKAEAIEF
                     FPLEEARIEPAAAQVLNRSDGVSLYL"
BASE COUNT         1859 a         3897 c         3743 g         2109 t
ORIGIN      
        1 gtcccgatcg acggcagcag gtcggccgct tcggccagga agacctgcag caggtcggca
       61 tcgagttcgt cgcgcacgcg cgtggccgcg gccacttcgg cctcggtttc agccaccgta
      121 gcaacttcaa tcgggctggc agcactggcc ttggccggga cgtcggggac attggcggca
      181 ggggtcggca cgtaaaccgg aattgcttcg gtaaccacga ccggcgccgc ggcttccacc
      241 acagacaccg cgaccgtatc tgctgtcgaa accgcgtcca caagcggtgc gggctgggcc
      301 gccgccaggt cgccggcttc cgagggcgtg ggttcgatca gcgtgatgtt tgcccattgc
      361 ggtgcttcga cctcacgggt ggccgacgga tgcaccacca cgggtgtcag ctcgtcatgc
      421 agtgcgctgt gtgcccgcac gatcgccacc acgtcctgca aggctcccgc atccagcggc
      481 gcctcgtccg gatacacgcc agccgcgaac tgatgcagca tgccgcgcat gtgctcgatg
      541 acgcgctcga gcgtgtcgaa ctgaggcgcc gtcagcgcat agtgccgggg acccgacagc
      601 atcgacagcg tatgcatcag gtcatcaagc ggatcggaaa tcgcaatgac aggtaaaaga
      661 cccaccgtct tggagatgcc cgacagcgag tgggcacgac gcacgacctg cgccgacacc
      721 ggccgtgagg gctcgtagcg ccattcgttg atgtcgtgcg cgagcacgcg cacgcactcg
      781 tcggcctcgt tcaggaagac cgagtacagc ccgtggctga tctccagcgg gccaatgcgg
      841 cgcacgtcct cgtcgtcgat gccgctcgac tcgaccggta tcgcttccag gaccacgtcc
      901 tcgtcgggcg ggaccagctc cggtagttcg aactgcatct cgagcgcggc ggcttcggcc
      961 gactgaaccg aatcctcctc atcggtcgct gcctcgcgcg aacggacatc ggcgccctcg
     1021 gccggccgtt ccgcgtcgct gtccaggtcg ccgagatcga agtcgaggct cgccgccgtc
     1081 gccgacagcg atgcgacttc ctgcggggct ttgaccggca gcgcgacgtc ctgcaccgcc
     1141 ggcgcgctgc catcaggctt tgggggcgtg tctcccggcg ccagcgccta gatctcgaac
     1201 atgccgcctt cgcgcacgcg ctgggaggca acgacgagcg gcgtcggatc gatgttcgcg
     1261 aacggatcgc ggtcgatggt ttcgacccag gcccggatta ctctctgagc accgttcgcc
     1321 aggtcgatca ggtcatcggt cgccggccgt tcctgcgcca gccagagatt gaagcactgc
     1381 tcgactcccc acgcaccctc gccgatgtgc ttgaaaccga ccatgcggct cgaacccttg
     1441 agcgtgtgga aggcgcggcg caccgttgtc agcgtggcct tgtcatcgcg ccgggtacgc
     1501 aggtcgttca ggttctcgac aatgctgtcg aggacctcgt gcgcctcctc gacgaagatt
     1561 tcgcggagtt cgctgtcagc tgcggcctgg gtagagggca gcggcgcggt cggcggcggc
     1621 gcctcggggg ccttcgccgg cgcgagcagc tgctccagtt cgacagcgtc ctcgcgcacc
     1681 ggcaggtcct tgagctgcgt gagcagctgc aacgcgcgcg cggccttggc cttgagctgc
     1741 tcgttgtcga gcagatcggc ctcgttcgaa agcatcggca gcagccggtc gagctcgccg
     1801 atggcacgcg cgtccccggc aacatgggtc aggcgctcgg ccagatgatg tgcggcttcc
     1861 agcgtcttgc gcgcggccgt ctcgacgttg tcggtacggc gcggttcggg cacgcgcatg
     1921 tcgatcgtgc gcggtgccgc ctggtattcc tcgtcggctg gtgcaaatac ggatgcgtcg
     1981 gcgggtagct ggcccagttc ggccgtgaac acgcctgtcg acgggtcata gtggaacatg
     2041 ccgcgcgggc gctcggtgtc ctggcccagg ctctcgacga agaagcccac ggccccgagg
     2101 ttctgcgcga tgcgcccgaa ctcgtccggc tccggcgcaa tcttcgcatc ggcaaagcgg
     2161 ccgatcgact cctgcacgtt gcgcagggcc gcgaccggat cctcgtagcc cagcagcgac
     2221 agcacgccgc agacctggtc gaacatcgcg gtggtcgccg gcaggtcgct acgctcgagc
     2281 ggattgcgga agaagcgatc cagccgctgt tcgatctcgc gcagcgtgga ctgcgtttcc
     2341 gccaccacgg tgcccatggt cagccggtcc tgcgcgcgcc gcgccagctc ggacatccac
     2401 ggtccggcct cgggcagcgg ttggccctgg cgcgccttca gcaggcgttc gatgacgagc
     2461 tgggcacggc cctcgtactc gtcgtccgtc agcggcaggt cgtccacgcc aatgtcggta
     2521 aacagcagtg ccgaggccac ttccagcccg agacgctcgc gcacatcggc cggcagcttc
     2581 gcaaagtcct tcgtgacctt gccgatgatt tccacagtcg tgccgacagc gcgtgccgac
     2641 agtcgagacg ccgatgtccg cgccagcgtg atttcctgct cgaacttggc ggtctcgggg
     2701 gagccagcga cgatctggcc ccacaactgc ttggcagacg caagcgcttc cttgatcgag
     2761 cgcagcgcgt cggcatcaac cgccgtcaac gaggcgcgct cgaagtccgc cgggatcagc
     2821 gcatcgagcc catacaggcg cttgatctcg gcgacgcggg ccggaccgcc ctgcgcacgg
     2881 ccaacgtagt acagcgcgtc gatcatcagg cgctcggcga ccgccccgcc gccttcgatc
     2941 atgcggcgca actgcagatt cagccgtgcc agcacgcgct tgaggtcgac gtccacgggc
     3001 acgacattcg agtccagtcc ttcgagcagc cctcgcacca cccaccagaa gctgcgtgca
     3061 agaccgcgct gcggcaggtg ttcaagatcg gccagcgctt ctctcatgcg cttgcgcgcg
     3121 ccttcgtcat ccgagttgcg caggaatccg agcaggccca gctcgaaacg cgcgcgccgg
     3181 tcacgcagct gatcgggcgg gtagttgcgc gcctcgatct gatggaacgc cggacgccgt
     3241 gacaggtcgg ggaagatcag gtcggccgga tgtacacggt tggcgcgatt gagctgcagg
     3301 acgtcgcggt agtacgggaa cagccggatc ggctggttgg ggcggccgga gagcagcgcc
     3361 tcgaggtacg cgacgaccgc ggtcagcgag gtttcaaccg agcgcacggc cgccggcagg
     3421 cagtccttgg gttcggcctc gaagcggcgc gtcagctgct cgaccgcatc ggtcacgagg
     3481 gcgacaccgc gcaggtcgag caactgcagg gcaccgttgg cctggtggac ctggttacgc
     3541 gcattgcgca ggctgtcgac gtcctgcttg ttggcgagaa acgcctttag gccgttgacg
     3601 gcctcggtca gcgagttgcg gatctcgtcg atgacccagg cgagcggcgc gatgtccggc
     3661 gccgcgccga ccagctggtt tggatcgtgt ctctccgcca tgtagtcctc gcctaatgcc
     3721 tcatgaccgc gagtgtcccg ccgttacgtc gcggccactg acgaaaattc gaccggctct
     3781 gccggcgact tttcgcaaac caggcgctca caagtaggtt ccacacttgc gcgctacccc
     3841 acgcggaacc gcgcgaccga ggctttcagc tcccgcgcca gcgtcgccag ctggccgatc
     3901 gactgtgccg cctggcgcgt gccgtgcgag gtctgctccg ttaccgccag aatgcgctcg
     3961 atcgattgcg ccacgccgcc ggccgagctc gcctggtcat tcgtcgtcga tgcaatgcgc
     4021 tcgatcagtt cagccagctg gcgtgagacg cggccgatgt cgcccagcgc gttgccggcc
     4081 gcgtcggaca gcttcgtccc ttcgaccacg ccctgcgtac tcttctccat ggccgccacc
     4141 gcgtcctgcg tgtcggtctg aatcgtgcgc accagcgcac cgatctgctt ggccgcctcg
     4201 gcggaacgat cggccaggcg ctgcacttcc tcggccacga ccgagaagcc tcgacctgca
     4261 tcgcctgccg acgccgcctg gatcgcggcg ttcagcgcca gtacgttcgt ctgttcagtg
     4321 atgtcggtga tcagttcgac gatttcgccg atctcctgcg aggactcgcc cagtcgcttg
     4381 atccgcttcg ctgtttcctg gatctgctcg cggatctcgt tcatgccggc gatctggttg
     4441 aacaccgcgc gctggccgcg ttccgcggcg tccagcgagg cgcgtgccac cttggccgac
     4501 tcgcccgcgc tcttggacac gtcgttgatc tgcgacgcca tcttcagcac cttgtcgccg
     4561 gtgtccttga tctccttgga ctgagcttcc gatgccgtca gcaggcgtgt cgcgatgtcg
     4621 cgtgcagcgg tcgatgcggt gttgacctgt tcggccgtgt tgttgatgcg acctaccagg
     4681 ccccgcaact cctccaccgt gtagttgacc gagtccgcaa tggcaccggt gatgtcctcc
     4741 gacaccgtcg cctgcaccgt caggttcccg tccgcgacct cctgcagctc gttcatcaac
     4801 cggagaatgg cggcctggtt ctgttcgttg gtgcgcttgg cttcctgctc gaggcgttcg
     4861 gcctgcatcc gctggatctc ggcttccgcc gcgcgcttgc gtgtgtcttc gagcagtacc
     4921 tttacgatcg cgaaggcgca tagcagcagg ccaatcgcga acagcgccat gatgaagagg
     4981 ttaactttgc gagtcgcctg ttctttcgta tagaggccac ccagttcggt cacctgctgg
     5041 cgcagctgct cggagtcgcg caggatggcc agctcggctt ccttcccttg cacgatcgct
     5101 tgcaggtttc cgatcgcgcc ggcaatcgag gcctggaatt cgccgtacac ccgcgtgagc
     5161 tcctgcagcc gcacgcgcgt ctcgctgtcg gtgctcgccg ccaggcgcag ggtgtcggaa
     5221 ccgacgagca gcgcctgcgt caggtcgcgg aactgattga tgtcgcgtcc gagcgtcagc
     5281 gcgacctccg cgtttgcgga actgccggcc accagcgagt tcgcgctctt cccgagccgc
     5341 tgcgtcagcg tgacgagctg ggcggccgtg gcgatctcgc gcgccggggc accggactgc
     5401 agcttcagcg cggtgatttc ctcggtcagc tgctgcaggc gcgggctggc atcgttgatc
     5461 ttcctgatca cgtcgccgaa cgcgagcagc gccttctcgt tggcgatgat caccgaggcg
     5521 gccgcctcgc tcgtgcgcca gcggtcgatc agcttggtta gcgccggctg catgtccgag
     5581 gccgtcgcgg acaggttgcg cagcgtgtcg ccattggcca gcgcgtcgag gttggccgcg
     5641 atcgcgtcgc ggctctcgcg cagttcgacg aaagcctccc ggacgccctc gacggcattc
     5701 ggggccgcct ttgccagccg ctgcgtatgc atcagcgtgt cgccgacgat ttcggtctgc
     5761 attgcgccgt tggcggcgag ccggttgtcg aagaacagca ccgccaccag cacaacaaag
     5821 cagaagccga gcaggatcaa cagcacctgc acctgggtgt tgaccggctt gtcaccgatc
     5881 agaggcagct tgacggcgag cagccggtcc accagccgca tcgtcatgcc ctcgttgccg
     5941 cgatcgatca tcacggtctt tggcggcggg ctcgccgcgc cgcggacggg ctccgcccca
     6001 tccctgctct tgcgctcgcg cgacttgcgc ttgaagagcg aacccacttc ctcgagtgcc
     6061 atctagtcct ccgccatatc ctcaacccgg cttggccggg ccatgctctc cgccgccatc
     6121 gacggcgcta cgcgttccct gcacggcagg ccggcgcgcg gcctcctgcg ctcaagcatt
     6181 gatcgccgta aaggccggtg ctgccgccac ttcgcgcaga tcgagttccg tcagcatcag
     6241 tccctcgagg tctcgatagc ggctgcgttc ccagcccgag gccgataccg tggtgtcgac
     6301 ggattccaga tccttcaggt ttcggatccc gaacgcccgg gtgatgagaa ttcctgccgc
     6361 gactccgagc ccctcgccga gcaccagcaa ctgctgtccc tgctcggctg caaggggctc
     6421 acgtcccatc aggtgctgca gatcgatgac tccgaccagt cggccgcgga cattggccag
     6481 cccgcggtac cacggcttgg tccacggcac gcgcgcggtt tcgggcagcg acacgatctc
     6541 ggccgcgagt cccatctcga ccaggtaatt ctcgacgccc atgcggacgg ccaggcgcgt
     6601 cggctccgtc ggcaggttcg gcgcttcctt gaggcgttgc gccagctgcg tcgagaactc
     6661 gcgcagcttg accagctgtc gtggactgac gtcgcgttgg gtagccatgg aggtgaggtt
     6721 cagccgagcg ccgtgatctt ctcgaacagc tcttcgggct tgaccggctt ggtgatgtag
     6781 tcacgcgcac cctgccgcag gccccagatg cgatcggtct gctggttctt gctggtacac
     6841 atgatgatcg gaatgtcctg cgtgaccggg tccttggaca gcgcgcgcgt cagctggaag
     6901 ccgttttggc ccggcatgac gacatccatc aggatcaggt cgggcatgac cgccttgacc
     6961 ttcatcatgg cttcctcggc gttctccgcg gtcgaaacgg catagccctg cttggtcagc
     7021 agttcattga ggaagaaacg ctcggtcggc gagtcgtcga cgatgagtac tttcttgata
     7081 ggcattggcc ccccgctaag gcccgagatc agttcattga atgctgtttc acggccgaca
     7141 gcaggctgtc cttcgtgaac ggcttggtaa ggtactcact cgagccgacc atgcgaccac
     7201 gcgcacggtc gaacagtccg tccttcgacg acagcatcac gaccggcgtc ttgtgaaagc
     7261 gcggactgcg cttgatcagc gcgcaggtct gatagccatc cagccgcggc atcaggatgt
     7321 cgcagaaaat gatgttgggc tggtgatcgt tgatcttcga cagcgcctcg aagccatcct
     7381 gcgccaggat cacctggcag ccggcctgcg aaagaaagat ctccgcgctg cggcgtatgg
     7441 tgttcgagtc gtcgatcacc attaccttct tgccgcggat atccgagaaa tcctgtgccg
     7501 ccatgtcgtg tcctcgatcc ttaaccggcc cctggacgcc gccggtcaat gccaagcggg
     7561 actctccgtg gcagaaactc gcgaacgatg taatcgaccg atcgtcgagt ttgtatacga
     7621 agatgcaagc acacgccgtg tcgaaaaccg cggttccgcg gtcagatttc gaccatttcg
     7681 aaatcttcct ttcgcgcccc gcactcaggg catgtccagt tcggcgggat ctcttccaac
     7741 cgcgtcccgg cagcaatccc cgactcaggc acgccggcgg cctcgtcgta gatccagccg
     7801 cagatcagac acatccaggt tttcgtcccc acgttggtcg tcatcatggc tcaggaactt
     7861 gagcacctaa tcggtcgctc agcggctaaa atcaaacact tagccgagga tcataacgtg
     7921 ggctcatagg tctccgctac cgccttcgcg ctagcgtcct gtgcccgcgt agcatcgttt
     7981 cttttgcgac acattttcga accccacgca tgccgacgct tcccgttgta ctcagctttg
     8041 gcgtgtccga tgcaaccggt gccgccggca tgcaggctga ccagcttgcg attgccagca
     8101 tgggatgtca tccggcgagc gtgatcactg cgatcgcgac cgccgaggac cagtttgcgc
     8161 acgactggct tgctctcgac gaggaacttg tcgagggcca ggcgcaggcg gtgctacaga
     8221 acatggcggt ggccgcattc aaggtcgggg cgatcggcag tgtcgatcag atgcagtcga
     8281 tcgccagcat cctggccgac tacgacacgg tgcccgtcgt gctcgatccg gtgctcgatc
     8341 gcaccgacga tgacgacgcg agcggcgaac tggcggctgc attgcgcgag ctgctgatgc
     8401 cgcagacgac catcgtgacc gtgaatctcg cgcaggcgcg tcgactcgtc agccttgccg
     8461 atgacgacga ggaccgcagc cacgacctgc tcgcggccgc gtgcgcgcgc gagatcatcg
     8521 gctggggtgc cgaattcgtg ctcatgaccg atgccgagcc gggatcagcc ctcgtgatca
     8581 atgcgctgta cgacgaatcc gggatggtgc gcagcgattc actgccgcgc gtggacgcgg
     8641 tgtcgacacg cttgcacggg gccggcgaca ccctgtccgc ggcactcgcc ggattgctcg
     8701 cgcagggact cgatgtcccc gaggcggccc aggaagcctc gcaatacacc gccgcggcac
     8761 tggtgcacgc cttcagctca ggcatcggcg tggcgattcc cgatcggttg ttctgggccg
     8821 gcgacgacga tgacgacgat gaccgcagca gcgatgtcaa ctgatcggcg ggatcgggct
     8881 gaccgcgtcg ccggtgtcta tctgctcaca ccggacacgg ggccgggcga cttcgaggcg
     8941 atggccgctc gcttcgaggc cgctgtggct gccggcgtct ccatagtcca gtaccgcaac
     9001 aaggcggcat ccgccgagga tcggcgcgcg caggcccggg tcctgcaggc tatcgcgcgc
     9061 aagcacaatg cgctcttcat ggtcaatgac gatatagacc tggccatcca actcgaggcc
     9121 gacggcgttc accttggccg gaatgacggc gacattgccg ttgcccgcca acgcctgccg
     9181 cggcagctgc tcggcgtgtc ctgttacaac gaccttgaga acgcgcgtcg cgcggtcgac
     9241 gccggcgcgg acctgctcgc cttcggatcc gtctttgcgt cggtcaccaa gccagccgcg
     9301 gtgcgcgcat cactcgggct gctgcgtgac gcacgcgagc ggtttccgca gcaacgtgtc
     9361 atcgcgatcg gcggtatcga cgcgaccaac atcggttcgg tcagcgccgc cggtgcgcac
     9421 gccgccgcac tgatcagcgc cgttttcgat gccggggatc cgggccaggc ggtccacagt
     9481 ctgcaaaagg aattcaggga agggttgatt cagcatgaca cgcaacgaac agctttttga
     9541 gcgcgcccag cgatcgattc cggggggcgt gaactcgccg gtgcgggcct ttcgttccgt
     9601 cggtggcacg ccgcgcttca tcgagcgtgc agagggcccg tacttctggg atgccgatgg
     9661 caagcggtac atcgactaca tcggctcatg ggggccgatg atccttggcc aggtccatcc
     9721 ggccgtcgtg aaggcggttc aggaggccgc agcgcgcggg ctttcctatg gtgcaccgac
     9781 cgaggccgag gtcgtcatgg ccgaggagct gctgaagctg gtgccatcga tgcagcagtt
     9841 gcggctggtg tcgtcgggca ccgaagcggc aatgagcgcg atccgcctgg cgcgtggcgc
     9901 cgccggcgac ggcaagggcc gtcgcaagat catcaaattc gaaggttgct accacgggca
     9961 cgccgacagc ctgctggtga aggcgggttc ggggctgctg acattcggca atccaagcag
    10021 cgcaggcgta ccggccgaga ttactcaaca cacactggtg ctcgactaca acgagccaca
    10081 acaactggaa gacgccttcc gccagcacgg tgacgacatc gcctgcgtca tcgtcgagcc
    10141 gatcgcgggc aacatgaacc tggttcgctc gaccgccgag ttcctgcgga cgatgcggga
    10201 attgacggcg aaacacggcg caatcctcgt gttcgacgaa gtgatgtcgg ggttccgcgt
    10261 cgcgctcggc ggcgctcagg cgttgtacgg catcgatccc gacgtgaccg tgctcggcaa
    10321 ggtgatcggc ggcggcatgc cggtggcggc ctttggcggc aagcgcgaga tcatgcgaca
    10381 cctcgccccg ctcggaggcg tctaccaggc gggaacactg tcgggcaatc cggtcgccgt
    10441 ggcgtgcggc ctggcgaccc tgcgcgagat ccagcggcca ggcttctata aaacgctgag
    10501 ccagcgcacc cgggacctcg tcaccgggct gacggccgcg gcccgttccg cggcacccgg
    10561 ccttgcattc tcgggcgata gcgtcggcgg catgttcggc ctctacttcg cggccgagcc
    10621 gccggcaacc tatgcgcagg tcatggcggg caacaaggag cgcttcaacc ggttctttca
    10681 cgccatgcta gagcgcggcg tctacctggc cccgtcggcc ttcgaagccg gattcgtctc
    10741 gatggcgcat gatgcggcgg tgatcgatgc gactcggact gccgcgcacg acgcattcgc
    10801 ggctattgcc gaattggctg ccggctaagc cgtttggccg ggaaccattg aggctcccga
    10861 tagacgaaaa tcacaccatg catacgcgac gactcctcat ccctttcctc accgccgccc
    10921 tgctgtcgct gctgacagcg accgccgcgg cgcagtccct gccacgcttt ggtgccccgg
    10981 atccgcacgt gaaggcggaa ctggtcgcgg cgaccgacgc ggtcgtgccg ggccagcccc
    11041 tgcaggtcgg cctgcggctc aagcacgaga gcgaatggca tacctactgg caggtgccgg
    11101 gcgattccgg cctgccgacg cgcattgagt ggcagttgcc tgccgggatg accgcgggac
    11161 cgatcgagtg gccgcacccg catcgcctgc ccgtgggacc gctgatgaat ttcggctacg
    11221 agggtgagac cttgttgctg accaccgtac aggtgggcga cgtcaaggcg gggacgcccg
    11281 tcacactgaa cgccaaggtc gaatggctcg aatgcaagga tgtctgcatc ccgggcagcg
    11341 cggacctgag cctgacgctg ccggtgaagg catcttcagc cccgtcgtca catgcaggtt
    11401 tgttcacggc cacgcgcagg ctggtgccgg tgacggtggc gggcctcgcg gcgcgcggca
    11461 cgatcgagtc caaccgcatc cgcctcgcct tcgatctgcc gtcgggcaag aaagccgagg
    11521 cgatcgagtt cttcccgctc gaggaagccc gcatcgaacc tgccgccgcg caggtgctga
    11581 accgcagcga tggcgtgtcg ctttacct
//
LOCUS       DATIRT010000517         7728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_485875, whole genome shotgun sequence.
ACCESSION   DATIRT010000517 DATIRT010000000
VERSION     DATIRT010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7728
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485875"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1074)
                     /locus_tag="VLN25_11825"
     CDS             complement(28..1074)
                     /locus_tag="VLN25_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007737656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSQ11280.1"
                     /translation="MAAMPPAPVRVAILAFPEVTASVLYGLYDLFLSAGRDWGTIVDG
                     RPGPSPMRPTVVSRFAESFTAANGVRLTPQQTLDECVAPDVICVPEVAIPPGQPLDGF
                     AAEVAWLKRCHANGATIAAACSGAMLLAQTGLLDDYDATTHWAYCDVMRTRHPRIRVH
                     PQRALVVTGVGQRLVMAGGGTSWLDLAIYLISRFVALEVAMQTARVNLIDWHDIGQQP
                     YARLARSRQVEDAEIARCQTWIAHHYDEPAPVAAMVRLSGLPERSFKRRFQQATGMSP
                     LEYVHTLRLEEAKQMLESGDAPVEAIANEVGYEDAGFFSRLFRRQVSLTPAQYRKRFG
                     AMRRALQSAGTASA"
     gene            1223..2023
                     /locus_tag="VLN25_11830"
     CDS             1223..2023
                     /locus_tag="VLN25_11830"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HSQ11281.1"
                     /translation="MPSPLDLLLDPISLAVMAIFGALVAWEALAPARKLPTVRGWKLM
                     GLGAFVVYFFVSSYLPLLWTESLAQFQLFDLSALGTWGGAAVGLLVYEAIAYGWHRSM
                     HTFTPLWRSFHQMHHSSERLDTYSAMWFSPLDMVGWTFASSLALTVVVGLTAEATTVV
                     LLTVTLLAILQHSNIRTPQWLGYFVQRPEAHSYHHERGVHKRNYSDLPIFDILFGTFH
                     NPRDFAPVNGFYDGASARIVDMLLMRDVSTPKARTKLNGELQPVGAND"
     gene            complement(2149..2886)
                     /locus_tag="VLN25_11835"
     CDS             complement(2149..2886)
                     /locus_tag="VLN25_11835"
                     /inference="COORDINATES: protein motif:HMM:NF039257.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tectonin domain-containing protein"
                     /protein_id="HSQ11282.1"
                     /translation="MALRTLLALTLAFASSLAQAQWQLLPGLATDIGVGARGDAWVTG
                     IDRVDGGHSIYRWDGGAWQIVGGGAERLDVDPQGNPWVINASGQVLRLERGGWQLLPG
                     LARDIGIGADGSVWVIGITRAPGGYTIHRWNGNDWDLVPGGAVRIDVDPQGTPWVVNE
                     RGDVLRRSRNGTWQRLAGRGTAVTVANDGTAWLLGRDRVPGGFSVQRWTGNGWRRAPG
                     GAVALSAGPVPWLVNADGNVFRWDGRR"
     gene            complement(3061..4299)
                     /locus_tag="VLN25_11840"
     CDS             complement(3061..4299)
                     /locus_tag="VLN25_11840"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HSQ11283.1"
                     /translation="MADRTFDLVLYGATGFVGRRSAAYLAGRRMRRKLRWAIAGRDAG
                     KLAALREELASDPAAVALPAIVIADSSDAAAVEAMAASTRVLITTAGPFALFGDAIVD
                     ACVQHRTHYADITGETAWARRVIDRHHKRAARDGTRIVPFCGFDSVPSDLGTLLVVRH
                     IEQTFGAPCVEVKGYFRMMGGFNGGTLASNAHRYESGETEAGRNPFLLNPRARHSASE
                     LLNAQDPRGARYDELVGSWVAPFIMGPINSRVVRRSAALNAAYRTPYGPGFRYQEYTK
                     FGPPLAAAKATAMAAAMRGFERLMEQGTARALLGKVLPKPGEGPSEASMASGWFKTDL
                     IGRTADGRTARARIAYAGDPGNRATLRILCEGGLALAFDGSKLPGAPKRGGVLTPATA
                     IGETLVPRLRAAGFEISIGR"
     gene            complement(4302..5126)
                     /locus_tag="VLN25_11845"
     CDS             complement(4302..5126)
                     /locus_tag="VLN25_11845"
                     /inference="COORDINATES: protein motif:HMM:NF036878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HSQ11284.1"
                     /translation="MSLTYILVDFDNVKPTAADIGMARGDKFRLAVVRGPQQTRYEAD
                     LAEALHSMGSQVTFTRCAKAGKNAADMQIAFLLGELVATMPASAQKSVRFVVISRDRD
                     FDPLLMYLHDKGFDATRAASFKAALGGKAAAEPEAKPAPRTRVAKAASKSAGAKAVQA
                     RPAAVKKAAAKKAPAAKKAALVQPAPKKAAHAAARTVSKRPSAPDPLVRVIEGLQRKG
                     DERPGKRKGLERFIESHLGRNLAPGQLETLIAQLEREGVVVFKDNKVDYQLPKSKK"
     gene            complement(5421..5621)
                     /locus_tag="VLN25_11850"
     CDS             complement(5421..5621)
                     /locus_tag="VLN25_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11285.1"
                     /translation="MSKRLLASITLAAAVAALSGCVVVPAYPAYGYGHGYGRVRPPPA
                     VIVTPGPDYRYRGGGYHPRPRY"
     gene            complement(5788..6087)
                     /locus_tag="VLN25_11855"
     CDS             complement(5788..6087)
                     /locus_tag="VLN25_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11286.1"
                     /translation="MQVVEMSHTPTLEAEVRLNEVERMVHQTARSAAKLVRQSMHEAI
                     TAATLVRSSMKEAFGAIVRAMRNIAKEVAVARQAVMPVATTMKRPVRKAGARLAA"
     gene            6478..>7728
                     /locus_tag="VLN25_11860"
     CDS             6478..>7728
                     /locus_tag="VLN25_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HSQ11287.1"
                     /translation="MSFQDLYIQKRTSAADAVRQVRDGDFIIVPTGVGEPPTLLTALS
                     EQRRDFHDVKVAQILAIRKFAYIDAATVDHVRHVAFFYGGATRAGGQAGWIDCIPNYF
                     SDIPRLIERGQIPADVVFTMASPMDAHGYFSLSLGADYTMAAVAKARAVVLEVNSNVP
                     FAFGNCHVHVSQVTALVESSEPVLEVGLPKIGPVQEAIGKYVADLIDDGSTLQIGYGG
                     IPDAVVMQLTHKHDLGIHTEMIGDGILTLVESGAITNRRKNYLPGKMIATFALGSSKL
                     YRFMDRNPVLEIHPVDFTNDPSHAGLNENLVAINAALQIDLLGQCGSESLGHVPYSGT
                     GGQSDFVRAANLSRGGKAFIVVPSTAKNDTISRIVPTLAAGTHVSTSKNDVNDFVTEY
                     GVAQLRGKSAKQRARELIAIAHPAF"
BASE COUNT         1288 a         2634 c         2464 g         1342 t
ORIGIN      
        1 gaagtcgctc cagcagattc cccgcaatca ggcggaagcc gtgcccgcgg actgcagtgc
       61 gcgccgcatc gcgccgaagc gcttgcgata ctgagcgggt gtcagtgaca cctgcctccg
      121 aaacagccgg ctgaaaaagc cggcgtcctc gtaacccacc tcgttggcga tcgcctcgac
      181 cggcgcatcg ccggactcga gcatctgctt ggcttcttcc aggcgcagcg tgtgcacgta
      241 ctcgagcggc gacatgcccg tggcctgctg gaagcgccgc ttgaacgaac gctccggcag
      301 gcctgacagc cgcaccatcg ccgccaccgg tgccggttca tcgtaatggt gcgcaatcca
      361 ggtctggcag cgcgcgatct cggcatcctc gacctgacgc gagcgggcca gccgggcata
      421 cggctgctgg ccgatgtcgt gccaatcgat cagattgacg cgggctgtct gcatcgcgac
      481 ctcgagcgcc acaaagcgtg aaatcaggta gatcgccaga tcgagccagg aggtgccgcc
      541 gcccgccatc accaggcgtt gcccaacgcc ggtcacgacc agtgcgcgct gcggatgcac
      601 gcgaatgcgt ggatggcgcg tgcgcatgac atcgcagtag gcccagtgcg tggtcgcgtc
      661 gtagtcgtcg agcaggccgg tttgtgccag cagcatcgcg cccgagcagg cggcggcgat
      721 cgtcgcgccg ttggcatggc agcgtttcag ccaggcgacc tcggccgcaa agccgtcaag
      781 cggctggccg ggcggaatcg cgacttccgg cacgcaaatg acatccggcg cgacgcactc
      841 gtccagtgtc tgttgcggcg tcagccgcac gccgttggcc gccgtgaacg attctgcgaa
      901 acgcgacacc accgtgggtc gcatcggcga cgggccggga cggccgtcga cgatcgtgcc
      961 ccagtcgcgt ccggccgaaa ggaagaggtc gtacagcccg taaagcaccg aggcggtgac
     1021 ctccgggaac gcaaggatgg cgacgcgtac cggtgcgggg ggcattgcag ccatcgaggc
     1081 tcctccttgg tgcgggtcca gatggccgtc gcgcggcgaa gtggccgaaa tggccggttc
     1141 gttgccgaaa cggctttagt acgcgcaggc atccggctct aacgtctggt ccgtcccgaa
     1201 ttcaaccaaa cggagcactg ttatgccatc ccctcttgat ttgttactcg accccatctc
     1261 gctggcggtc atggccatct tcggcgcgct ggtcgcctgg gaagccctgg ctcccgcgcg
     1321 gaagctgccc accgttcgcg gatggaagct gatggggctg ggtgcgttcg tcgtttactt
     1381 ctttgtttcg tcgtacctgc cgctcctgtg gaccgagtcg ctggcgcaat tccagctgtt
     1441 cgacctgagc gcgcttggga cctggggcgg cgccgctgtc ggcctgctcg tttacgaggc
     1501 gatcgcgtac gggtggcatc ggtccatgca caccttcact ccgctgtggc gctcgttcca
     1561 ccagatgcat cactcgtccg agcggctcga tacctacagc gcgatgtggt tcagcccgct
     1621 tgacatggtc ggctggacct tcgcgtcgag cctggcgctg accgtcgtgg tcggtctgac
     1681 ggcggaagcc accaccgtcg tactgctcac cgtcaccctg ctcgccatcc ttcagcacag
     1741 caacatccga acgccgcagt ggctggggta cttcgtgcag cggcccgagg cgcactcgta
     1801 tcaccacgag cgcggggtgc ataagcgcaa ctactcggat ctgccgatct tcgacatcct
     1861 gttcggcacg ttccacaacc cgcgcgactt cgcgccggtc aatggtttct acgatggcgc
     1921 atcggcccgc atcgtcgaca tgctgctcat gcgggatgtc tcgactccca aggcacgcac
     1981 gaagttaaac ggcgagctgc agccggtggg cgcgaacgac tagcgaagag gacccacaag
     2041 ggccaagtac cgcacgcaac tgccacagct cggcgaccgc cggttcatga ccgacggcgg
     2101 cggtctcgag accttttcac gacgactgtg acctaggaac gcggccgcct agcggcggcc
     2161 gtcccagcga aagacgttcc cgtcggcgtt gacaagccag ggcaccggcc cggcagacag
     2221 tgcgacggcg ccccccggag cgcgtcgcca tccgttgccg gtccagcgct gtaccgagaa
     2281 tccgcccggg acgcggtcgc gtccgagcag ccacgccgtg ccgtcgttcg cgactgtcac
     2341 cgcagtgccg cggccggcca agcgctgcca ggttccattg cgtgatcgac gtaggacatc
     2401 gccacgttcg ttgaccaccc atggagtccc ctgcggatcg acgtcgatgc gcaccgcacc
     2461 gcccggaacg aggtcccagt cgttgccgtt ccagcggtgg atcgtgtatc cgccgggcgc
     2521 acgcgtgatg ccgatgaccc acaccgaacc atcggcgccg atgccgatgt cgcgcgcgag
     2581 ccccggtagc agctgccagc ctccccgctc gagccgcagg acctgtccag acgcgttgat
     2641 gacccatgga ttgccctgcg ggtccacatc gaggcgctcg gcgccgccac cgacgatctg
     2701 ccaggcgccg ccgtcccagc gatagatcga gtggccgccg tcgacccgat cgatgcccgt
     2761 gacccaggca tcaccgcgcg cgcccacgcc gatgtcggtc gcgaggcccg gcagcagttg
     2821 ccactgcgcc tgggccagag aacttgcgaa ggcaagagtg agtgcgagca gcgtgcgcag
     2881 tgccatggtc cgtccagtcg gtggagtgcg ggaagtccat cctaacgaag gatgcgcctt
     2941 ccctgagacc ggccggacgg ctgctccttt tcgtggcaaa cattacatcg tgcgtgacaa
     3001 cacgctgcgc ccgatgctgc ccggtgcgca caacgtcggc gcgcacaacg tcggcgccaa
     3061 ctaacggccg atcgagatct cgaagccggc cgcgcgcagc cgcggcacta gcgtctcgcc
     3121 gattgcggtc gcgggtgtca gcacgccgcc gcgcttcggc gcacccggaa gtttgctgcc
     3181 gtcgaatgcg agcgcgaggc cgccctcgca caggatgcgc agcgtcgccc ggttgcccgg
     3241 gtcgccggcg taggcgatgc gtgcgcgtgc cgtgcggccg tcggccgtgc gcccaatgag
     3301 atcagtcttg aaccagccgc tggccatcga tgcttccgag ggaccttcgc ccggcttggg
     3361 cagcaccttg ccgagcagcg cacgtgctgt tccctgctcc atcagccgct cgaaaccgcg
     3421 catcgccgcc gccatcgcgg tggcctttgc cgcagccagc ggcggaccga acttcgtgta
     3481 ctcctggtag cggaagccgg ggccataagg cgtgcggtac gccgcattca gcgccgcgct
     3541 gcgccggacc acgcgcgagt tgatcggccc catgatgaag ggcgccaccc aggacccgac
     3601 gagctcgtcg tagcgggcgc cgcgcgggtc ctgcgcattc agcaattccg aggccgagtg
     3661 ccgggcgcgc gggttcagca ggaacggatt gcgtccggcc tccgtctcgc cggattcgta
     3721 gcggtgcgcg ttcgacgcca gcgtgccgcc attgaagccg cccatcatgc ggaagtagcc
     3781 cttcacctcg acgcatggcg ctccgaacgt ctgttcgatg tgccgcacga ccagcagcgt
     3841 gccgaggtcc gacggcaccg agtcgaagcc gcagaacgga acgatgcgtg tgccgtcacg
     3901 cgccgcgcgc ttgtgatggc ggtcaatcac gcgccgcgcc caggccgtct cgccggtgat
     3961 gtcagcgtag tgcgtgcggt gctgcacgca ggcgtcgacg atcgcatcgc cgaagagcgc
     4021 aaacgggccg gcggtcgtga tcagcacgcg cgtcgacgcg gccatcgcct cgaccgccgc
     4081 tgcatcgctg ctgtccgcga tgacgatcgc cggcaaggcc accgcggccg gatcgctggc
     4141 caattcctca cgcagtgccg ccagcttgcc ggcgtcgcga ccggcgatgg cccagcgcag
     4201 cttgcgccgc atgcgtcgcc cggccagata cgctgcggag cgccggccga cgaagccggt
     4261 cgcgccgtag agcacgaggt cgaaagtgcg atcggccatc gctacttctt cgacttcggc
     4321 agctggtaat cgaccttgtt gtctttgaac acgacgacgc cttcgcgctc aagttgcgcg
     4381 atcagagtct cgagttgacc cggcgcgagg ttgcggccca ggtgcgattc gatgaagcgt
     4441 tccagtccct tgcgcttgcc tgggcgttca tcccccttgc gctgcaggcc ctcgatgacc
     4501 cgcacgagcg ggtccggtgc tgacggtcgt ttgctgaccg tgcgcgctgc cgcgtgcgcc
     4561 gccttctttg gagccggttg cactagagcg gccttcttcg ccgccggcgc tttcttcgcg
     4621 gcggctttct tcaccgcggc gggcctggcc tgcacagctt tcgccccggc ggatttcgat
     4681 gcggctttgg ccacacgtgt gcgcggagca ggcttcgcct ccggttccgc ggccgccttg
     4741 ccgccgagcg cggccttgaa gctcgcggcc cgcgtcgcgt cgaagccttt atcgtgcagg
     4801 tacatcagca atggatcgaa atccctgtcg cgcgagatga cgacgaagcg caccgacttc
     4861 tgtgcgctgg ccggcatcgt cgccaccagc tcgcccagca gaaacgcgat ctgcatgtcc
     4921 gccgcgttct tgccggcctt ggcgcagcgc gtaaaggtca cctggctgcc catgctgtgc
     4981 aacgcctcgg cgaggtccgc ctcgtagcgc gtctgctggg gcccgcgcac gaccgcgagc
     5041 cgaaacttgt cgccgcgcgc catgccgatg tcggcggcgg tcggtttgac gttgtcgaag
     5101 tcgacgagga tgtaggtgag tgacatgcgg atcgcgggaa gtggaggcgg ctggactgta
     5161 gccgttaatt gggaggcggc gggcactccg cgtgcgcaca acaatgagat acggcgccag
     5221 caactccctg cctcctgtcc atggagggag tggagccgtc tggcgccgga actgcaactg
     5281 cgttgaacaa ttgcactatg gccggcgggc aggccgccgc cgcgccctac atcagcggcg
     5341 ttgcattcaa ggtagaacct tctaacggag aagcgcgacg cggcgtgacg gtgttcagct
     5401 cagtgcggtt accacgacac ttaataacgt ggccgggggt gatagccacc gcctcgatag
     5461 cgatagtccg gtcccggcgt aacgatgacg gccggcggcg gtctgacacg tccgtagccg
     5521 tgtccatacc cataggccgg gtacgcgggt acgacaacac agccggacaa agccgccacc
     5581 gcggcggcga gcgtgatgct agccaacagt cgcttcgaca tacgagcctc ctggagcagt
     5641 cgaccgacgt cgaatggcgt cagtggctat caaacggctc aacgcagcgg agtgccgacg
     5701 tgtatgtcgt aacaagcggt aacagcaaca ccccggcgcg cggatcaacg cgtaccgggg
     5761 ctaagcctgc gcgcaccgcg cggcggctta cgccgccagc cgagcgcccg ctttgcggac
     5821 cggccgcttc atggtcgtcg ctaccggcat cacggcctga cgggccacag ccacctcttt
     5881 cgcgatgttc cgcatggcgc gcacgattgc gccgaacgcc tctttcatcg aactgcgtac
     5941 aagcgtcgcc gcggtgatcg cttcgtgcat cgattggcgg acaagcttcg cggcgctgcg
     6001 tgcggtctga tgcaccatcc gctccacctc gttgagcctg acctcggctt ccaacgtggg
     6061 cgtgtggctc atctcgacaa cttgcacgcg cttctccgtg cctgaacgcc ttctcatcgt
     6121 ggtgcgtctc gtgaccataa aaactccttc tcaacgtgtc ttggtgccag cgcaacccgc
     6181 gccggacacc taccagtctg ggcagcgccc cgccgcccgc actgcgtcgc gtcagaagtc
     6241 gtccattgga gggggaacct tcaaaaatga aaccggcgcg gctatcaccg ccgccgtctc
     6301 acgccagacg gcgcgctcat tgtcgcctgg caatgcccgg caaagccttg ccttcggcgt
     6361 gggtcagcgc acagacgggc cgccgtagcc tgactatttt gggttgacgc tggcacgttg
     6421 cgccctctgc atgagcaact aagccccggc tgcgtgagtt gttgttcgaa taggaacatg
     6481 tcgtttcagg acctgtatat acaaaaacgc acttctgccg cagatgccgt gcgacaggtg
     6541 cgcgacggcg acttcatcat cgtgcccacc ggggtcggcg agccgccgac actgctgacg
     6601 gcgctttcgg agcagcgtcg cgacttccac gacgtcaagg tggcgcagat cctcgccatc
     6661 cgcaagttcg cctacatcga cgcggcgacc gtggatcatg ttcggcacgt tgcctttttc
     6721 tacggcggtg ccacccgcgc cggcggtcag gcaggctgga tcgattgcat tcccaactac
     6781 ttctcggata ttccacgcct tatcgagcgc gggcagattc ccgccgacgt cgtattcacg
     6841 atggcgtcgc cgatggatgc ccacggctac ttttccctca gtctgggcgc cgactacacg
     6901 atggccgcgg tcgccaaggc ccgcgcggtc gtgctcgagg tcaactccaa cgttccgttt
     6961 gccttcggca actgtcatgt gcacgtctcg caggtcactg ccttggtcga aagcagcgag
     7021 ccggtgctcg aggtcgggct gccgaagatc ggtccggtgc aggaggccat cggcaaatat
     7081 gtggccgacc tgatcgacga cggttcgacg ttgcagatcg gctacggcgg tatccccgac
     7141 gccgtggtca tgcaactcac gcacaagcac gacctcggta tccacaccga gatgatcggc
     7201 gacggcatcc tgacgctggt cgagagcggc gccatcacca accggcgcaa gaactacctg
     7261 ccgggaaaga tgattgcgac cttcgccctc ggctccagca agctctaccg cttcatggat
     7321 cgcaacccgg ttctggaaat ccatccggtg gacttcacca acgacccatc gcacgcggga
     7381 ctgaacgaaa acctggtcgc catcaacgcc gccttgcaga tcgatcttct tggccagtgt
     7441 ggctccgaga gtctcggcca tgttccctat tcgggtacgg gaggtcagtc cgacttcgtg
     7501 cgcgctgcaa acttgtcgcg gggcggcaag gcgttcatcg tcgtaccctc gaccgccaag
     7561 aacgacacca tttcgcgcat cgtgccgacg ctcgccgcgg gcacccacgt cagcacgagc
     7621 aagaacgatg tcaacgactt tgtcactgag tacggcgttg cccaattgcg cggcaagtcg
     7681 gccaagcagc gcgcgcgtga actgatcgcc atcgcacacc cggcattc
//
LOCUS       DATIRT010000518         8067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1371349, whole genome shotgun sequence.
ACCESSION   DATIRT010000518 DATIRT010000000
VERSION     DATIRT010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8067
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371349"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VLN25_11865"
     CDS             complement(<1..327)
                     /locus_tag="VLN25_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HSQ11288.1"
                     /translation="MSSREVYVSAALADAGDDAAIDAAFDAAEADEPLRRQFSVGGEQ
                     AGDRLDKVLAQWMPEVSRSRLKQWIEVGAVRVNGATVRPRHAVVAGDHVELEPQPAPD
                     AGAYTPE"
     gene            359..1144
                     /locus_tag="VLN25_11870"
     CDS             359..1144
                     /locus_tag="VLN25_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HSQ11289.1"
                     /translation="MWTVALTRRVVIVLAALALGGCSWLGLDSMFEKDATARWDADKL
                     YAEARAELSAGNCVKAREYYQKLESRYPFGRHAQQALMEIAYTYYKEGETAQAIQTAD
                     RFIRQYPNNPQVDYMYYLKGLASFNEDVGFLSKLMTQDISSRDAKGARESYDAFRDLV
                     TRYPESRYAEDARARMRHLINALAQAELNVANFYFDRKAYIAAIQRSQIVIRDFQTSP
                     VAEEALIVMARSYQALELPDLQADAERVLALNFPDSRYLRGRR"
     gene            complement(1141..3195)
                     /locus_tag="VLN25_11875"
     CDS             complement(1141..3195)
                     /locus_tag="VLN25_11875"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HSQ11290.1"
                     /translation="MSRPASAPAPDPVADDIARAFAAGGALSAAVPGFVPRASQADMA
                     ATVHQAILDGATLVAEAGTGTGKTFAYLVPALLAGGKLLISTGTKPLQDQLFRKDLPA
                     VLKALKINAATALLKGRANYLCLHRMERAEGEALLSTRDDARHLRAIVRFAAVTVSGD
                     RAELSEVPESAAVWPLVTSTRDNCLGAECPRFSACFVFKARRDAQAADVVVVNHHLFL
                     ADLALRDDAIRDFLPTVDTVILDEAHQVPDVAADFFGTTLSLAQGIELARDSRALGLS
                     RAADGASWAPLTARVEKAVRDVRLRLGDIGLAPGSRTPLARLPRDARLIETLDQLDED
                     AGELARAVEINRGRDPELDLLGPRVAELKRGLAEWSRAVGTRGAADSESGDDPNDGPG
                     EDPDEDPAVRWISISAHGAQFHKTPLAAGRLLARAREAQSQAWILTSATLTTGSRFDH
                     FIAELGIEARTARWDSPFDFARQAVLYLPQTMPSPYDARFPEHVAEAAWPLVQANGGR
                     AFLLCTTLRAVDRVAARLHQLMERDATPLPLLVQGSSSRRALLDGFRSAGNAVLVGSV
                     SFWEGIDIRGEALSLVVIDKLPFAPPDDPIVEAKIRYLRRCGRNPFNEYQLPEAVMLL
                     KQGAGRLIRDEHDRGVLMILDERVLTKSYGRTVLASLPPFARTRSEAEAIEFLTRRRE
                     TA"
     gene            complement(3192..3389)
                     /locus_tag="VLN25_11880"
     CDS             complement(3192..3389)
                     /locus_tag="VLN25_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003276168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HSQ11291.1"
                     /translation="MEQDEIKRRIIELQVEHRDLNDAIERLAIQPGVDELALRRLKKR
                     KLQIKDSVRLLEMQLVPDIPA"
     gene            3497..4273
                     /locus_tag="VLN25_11885"
     CDS             3497..4273
                     /locus_tag="VLN25_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HSQ11292.1"
                     /translation="MTSFKVNVCVAQHIGDRQEQQDRVAIITSPRNPGAMLAIVADGM
                     GGRTGGRMAADQVVSTAENLFKDMTERDSTFRDMLLHLAAEAHTVIRLTAISSEKEPH
                     STLCALIIKKNYAIWAHAGDSRLYFFRGSKLVHRTEDHTYAEQLRLEGRYEDAEIASG
                     RYKNILVSALGINKKPKIVIGEDAALREGDTFLLASDGMWAYFEDDELAEILQQLSPR
                     DASERLVSLARERAEGRGDNLSVAIIKLEPQPREAQRGRA"
     gene            complement(4279..5010)
                     /locus_tag="VLN25_11890"
     CDS             complement(4279..5010)
                     /locus_tag="VLN25_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11293.1"
                     /translation="MRTLILLIALLAAAIVSAQEFSGRYPPGSITTREQADQAIEAAQ
                     KEEVRLDAEFSAREAECYRGFLVNDCREKVRRDRELARRDVRRVELEAKDTRRRLDQE
                     DLARRRAEQDRQQALEAETKSAREAAARAESDKRTSKAVESAKGSLSDEEVARNRADY
                     ERKQKEHAARLEREKQDAAKRAQEAAAYQQKQAEAVKRAEASVAKRKRREERRAERKK
                     ALEQKEAEREAIRKRAEEAAAALPR"
     gene            complement(5042..6133)
                     /gene="zapE"
                     /locus_tag="VLN25_11895"
     CDS             complement(5042..6133)
                     /gene="zapE"
                     /locus_tag="VLN25_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HSQ11294.1"
                     /translation="MSFRPQLEAALAQRGYALDDSQERAAERLQRLHQDWTDYKSQRS
                     NALRKLLVRPPVPRGVYLWGDVGRGKSFLMDAFFATVPVIRKARVHFHEFMRGVHAEL
                     HELKGTAEPLDEVAQRIARRHRLICFDEFHVSDIADAMILYRLLDRLFALNVGFVITS
                     NYVPDALYQDGLHRDRMLPAIDLLKDRLEVVHVDGGVDYRRRAMERIEAYLTPWGAAA
                     EQALAAAFDRVAEAEDDDPVLIIESRTIRARRRAGGVVWFDFAVLCGGPRSQNDYLEL
                     AAQFHTVVLSNVPKMSAGQASEARRFTWLVDVLYDHRVKLLLSAEVPAAELYAAGALS
                     NEFTRTVSRLEEMQSRAYLESPRRAVGRV"
     gene            complement(6181..7620)
                     /gene="lpdA"
                     /locus_tag="VLN25_11900"
     CDS             complement(6181..7620)
                     /gene="lpdA"
                     /locus_tag="VLN25_11900"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HSQ11295.1"
                     /translation="MSKNFDVVVIGGGPGGYIAAIRAAQLGFSVACIDQWKNKDGGPA
                     PGGTCTNIGCIPSKALLQSSEHYEHAGHHFAAHGIEVKGLGLDLGKMLGRKDTVVRQN
                     NDGILFLFKKNKIAFFHGRGSFVGRSDAGIEVNVDAATSESLVARHVIVATGSNARPL
                     PGADFDESRILSNDGALAIPEVPKRLGVIGAGVIGLEMGSVWRRLGAEVTILEALPAF
                     LGSVDDQIAKEAAKLFARQGLKVHLGVRIDAVAVEKKGKSQEVRVTYADAKGDVQSLA
                     VDRLIVSIGRVPNTTGLNVEAVGLALDARGFVAVDDDCRTNVPNVWAVGDVVRGPMLA
                     HKAEEEGVAVAERIAGQHGHVNFNTIPWVIYTSPEIAWVGQTEQQLKAAGRAYKAGTF
                     PFAANGRARALGDTNGMVKFLADAATDEILGVHIIGPFASELIAEAVVAMEFRASAED
                     IARICHAHPSLSEATKEAALAVDKRTLNL"
     gene            complement(7649..7981)
                     /locus_tag="VLN25_11905"
     CDS             complement(7649..7981)
                     /locus_tag="VLN25_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HSQ11296.1"
                     /translation="MGTADEIGKLHALYKDGALTQAEFETAKAELFRRQSTDSTGDPL
                     KAAAEGANRLRLSDSDKWIAGVCGGLGKTTGVDSWIWRLLFALGLFLGGVTLVLYVLL
                     WIFVPREY"
     gene            complement(7985..>8067)
                     /locus_tag="VLN25_11910"
     CDS             complement(7985..>8067)
                     /locus_tag="VLN25_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoamide succinyltransferase"
                     /protein_id="HSQ11297.1"
                     /translation="DGREAVLSLVTMKEALEDPARLLLEV"
BASE COUNT         1383 a         2745 c         2569 g         1370 t
ORIGIN      
        1 ctcgggcgtg taggcccccg cgtcgggcgc cggctgcggc tcgagttcga cgtggtcgcc
       61 cgcgacgacg gcatggcgcg gtcggaccgt cgcgccgtta acccgcacgg ccccgacctc
      121 gatccattgc ttgagccgcg aacgtgagac ttcgggcatc cactgcgcaa ggaccttgtc
      181 cagccgatca cccgcctgct cgccacccac gctgaactga cgccgcagcg gctcgtcagc
      241 ctccgcagcg tcgaacgcag catcgatcgc ggcatcgtcg ccggcgtcgg cgagcgcagc
      301 tgaaacgtat acttcgcggc tggacaacgg acgtgcccca atgatggatg gagtgcctat
      361 gtggaccgtc gctctgacgc ggcgggtggt catcgtgctt gccgcgctgg cgctgggcgg
      421 gtgcagctgg ctcggactcg actcgatgtt cgagaaggac gcgaccgcgc gatgggatgc
      481 cgacaaattg tacgcggagg cccgcgcgga actgtcggcc gggaactgtg tgaaggcgcg
      541 cgagtactac cagaagctcg aatcgaggta tccgttcggc cggcatgcgc agcaggcgct
      601 gatggaaatt gcctacacgt actacaagga aggcgagacc gcgcaggcga tccagaccgc
      661 cgaccgcttc attcgccagt acccgaacaa cccgcaggtg gactacatgt actacctgaa
      721 ggggcttgcc tcgttcaacg aggacgtcgg tttcctgagc aagctcatga cgcaggacat
      781 ctcgtcgcgg gacgccaagg gggcgcgcga gtcgtacgac gctttccgtg atctggtcac
      841 gcgctatccg gaaagccggt atgcggagga cgcgcgcgcg cgcatgcgcc atctgatcaa
      901 tgcgctggcg caggccgaac tgaacgtcgc caacttctac ttcgaccgca aggcctatat
      961 cgcggcaatc cagcgatcgc agatcgtgat ccgcgacttc cagacctcgc cggtcgccga
     1021 agaagcactc atcgtgatgg cgcggtccta ccaggcgctg gaattgccgg atctgcaggc
     1081 tgacgcggag cgcgtcctcg cgctgaattt ccccgatagc cgctatctgc gcggacgccg
     1141 ctaggcggtt tcgcgccgcc gcgtcaggaa ctcgatggcc tcggcttcgc tgcgcgtgcg
     1201 cgcgaacggc ggcaggctcg ccagcaccgt ccggccatac gacttcgtca gcacgcgttc
     1261 atcgagaatc atcagcacac cgcgatcgtg ctcgtcgcga atcagccggc ccgcgccctg
     1321 cttgagcagc atcaccgctt ccggcagctg gtattcgttg aacgggttgc ggccgcaacg
     1381 cctcagatag cggatcttcg cctcgacgat cggatcatcg ggcggagcaa acggcagctt
     1441 gtcgatcacc accagcgaca aggcctcgcc gcggatatcg atgccttccc agaaactgac
     1501 gctgccgacc agcacggcat tgccggcgct gcggaatcca tccagcaagg cccgtcggct
     1561 gctcgagccc tgcaccagca acggcaatgg cgtcgcgtcg cgttccatca gttggtgcag
     1621 ccgcgccgcg acccggtcaa cggcgcgcag cgtcgtgcac agcaggaagg cgcggccgcc
     1681 attggcctgc accagcggcc aggcggcctc cgccacgtgc tctggaaagc gggcatcgta
     1741 aggcgacggc atcgtctgcg gcagatacag caccgcctgg cgcgcaaaat cgaacgggct
     1801 gtcccagcgc gccgtgcgtg cctcgattcc gagttcggca atgaagtgat cgaagcgact
     1861 gccggtcgtc agcgtggccg aggtcaggat ccatgcctgc gattgcgcct cgcgcgcccg
     1921 cgcgagcaga cgaccggccg cgagcggggt cttgtggaac tgcgcgccgt gcgccgagat
     1981 cgatatccag cgcaccgcgg gatcctcgtc gggatcctcg ccgggaccgt cgttggggtc
     2041 gtcaccactt tcgctgtccg ccgcacctcg cgtcccgacg gcccggctcc actcggcgag
     2101 cccgcgcttg agttcggcaa cgcgcggccc gagaagatcc agttccgggt cacggccgcg
     2161 attgatctcg accgcccgcg cgagttcgcc ggcatcctcg tcgagctgat cgagcgtttc
     2221 gatcaggcgc gcatcgcgcg gcaatcgcgc cagcggcgtt cgggatcccg gagccagtcc
     2281 gatgtccccg agcctgaggc gcacgtcccg aaccgccttc tcgacccgcg cggtgagcgg
     2341 ggcccacgat gctccgtcgg ccgcgcgcga cagtcccagc gcgcggctgt cacgcgccag
     2401 ctcgatccct tgcgccagcg acagtgtcgt gccgaagaag tcggccgcca cgtcgggcac
     2461 ctggtgcgcc tcgtcgagga tcaccgtgtc gacggtcgga aggaagtcgc ggattgcatc
     2521 gtctcgaagt gcgaggtcgg cgaggaacag gtgatggttg acgaccacca catcggccgc
     2581 ctgcgcatcg cggcgcgcct tgaacacgaa gcatgcggaa aaccgcgggc attcggcgcc
     2641 caggcaattg tcccgcgtcg aagtcaccag gggccacact gccgcgcttt ccggcacttc
     2701 cgacaactcg gcgcgatcac cggagacagt caccgcggca aagcgcacga tggcacgcag
     2761 gtggcgcgca tcgtcgcgcg tcgaaagcag ggcctcgccc tccgcgcgtt ccatgcgatg
     2821 aaggcacaga tagttggccc ggcccttgag caatgccgtc gccgcattga tcttcagcgc
     2881 tttcagtacg gcgggcaggt ccttgcggaa gagctgatcc tgcagcggct tggtgccggt
     2941 ggagatcagc agcttgccgc cggccaacag tgctggcacg agatacgcga aggttttgcc
     3001 ggtaccagta ccggcttcgg cgacgagcgt ggcgccgtcg aggatcgcct ggtggacggt
     3061 ggccgccatg tcggcctgcg acgcacgcgg aacaaagccc ggcacggctg ctgacagcgc
     3121 gccgccggcc gcgaaggcac gcgcgatgtc gtcggcaacg gggtctggcg ccggggccga
     3181 ggccggccgg ctcacgcagg aatgtcgggc acgagctgca tttcaagcag gcggaccgaa
     3241 tccttgatct gcagctttcg ctttttcagg cgccgcaagg caagctcatc gacgcccggc
     3301 tgaatcgcga ggcgttcgat cgcgtcgttg agatcgcgat gctcgacctg cagttcgatg
     3361 atgcgccgct tgatctcgtc ctgttccatg gcgcgtgcaa atcgtcaaag tggacccgaa
     3421 aaggctgcgg tacccggcgc tccggcagca gcgagaaagc cgatgataga ctccttcgac
     3481 atcccatcag agtcgcgtga cgagcttcaa agtcaacgtc tgcgtcgcgc agcacatcgg
     3541 cgaccggcaa gaacaacagg accgcgtggc catcattacc agcccgcgta atcccggtgc
     3601 catgctcgcg atagtcgcag acggcatggg agggcggacc ggcggtcgga tggcggccga
     3661 ccaggtcgtc agcaccgccg aaaacctttt caaggacatg acggaacgcg attcgacgtt
     3721 ccgggacatg ttgcttcatc tggccgctga ggcgcacacc gtcatccgcc tgaccgcgat
     3781 ctcctccgaa aaggaaccgc acagcacatt gtgcgcactg atcatcaaaa agaactacgc
     3841 gatctgggcg catgcaggcg acagccggct gtactttttc cgcggctcaa agctggtcca
     3901 ccgcaccgag gaccacacct acgcagagca gctgcggctc gaaggccgct acgaggacgc
     3961 ggaaatcgca tcgggccgct acaagaacat cctggtcagt gcgctcggca tcaacaagaa
     4021 gccgaagatc gtcattggcg aggacgccgc cctgcgcgaa ggcgacactt tcctgctggc
     4081 aagcgatggc atgtgggcgt atttcgagga cgacgaactc gcggagatcc tccagcagtt
     4141 gtccccgcgg gacgcctccg agcgcctcgt ttcgctggca cgcgagcgcg ccgagggtcg
     4201 cggtgacaac ctctcggtcg cgatcatcaa gctcgagccg caaccccggg aagcgcagcg
     4261 cggccgcgct tagctcagtt aacgcggcag tgccgcggca gcctcttccg cgcgcttgcg
     4321 gatcgcctcg cgttccgcct ccttctgctc cagcgccttc ttgcgctccg cgcgccgttc
     4381 ctcgcggcgc ttgcgtttgg cgacactggc ttcggcacgt ttgaccgctt cggcctgctt
     4441 ctgctggtac gccgccgctt cctgcgcacg ctttgcagcg tcctgctttt cgcgctcgag
     4501 acgcgcggcg tgttctttct gcttgcgctc gtagtcggcg cgattgcggg ccacttcctc
     4561 gtcggaaagc gatccctttg cgctctcgac cgccttggac gtgcgcttgt cggactcggc
     4621 gcgcgcggcc gcttcacggg cgcttttcgt ctccgcctcg agcgcctgtt gccggtcctg
     4681 ctcggcacgc cggcgcgcca ggtcttcctg gtccagccgg cgccgcgtgt ccttggcctc
     4741 cagttcgacc cgccgcacgt cacgccgcgc cagttcgcgg tcacgtcgga ctttttcccg
     4801 gcagtcgttg acgaggaaac cgcgatagca ctcggcctcg cgcgccgaga actccgcgtc
     4861 gagccgcacc tcctccttct gtgcagcttc gatggcctgg tccgcctgtt cgcgcgtcgt
     4921 gatgctgccg ggcggatagc ggccagaaaa ttcctgcgct gacacaatgg ccgcggcgag
     4981 caaggcaatc agcagaatca gagtacgcac aaggagtcaa cgttcggcgg gcatgatcgg
     5041 attacacacg acccaccgct cgcctcggtg attcaaggta ggcgcgcgac tgcatctctt
     5101 ccagccgtga cacggtgcgc gtgaattcat tggaaagggc gccggccgcg tacagctcgg
     5161 ccgcgggcac ttctgcggac aacagcagct tgacccgatg gtcataaagg acatccacca
     5221 gccaggtgaa gcggcgcgct tctgatgcct ggccggcgga catcttcgga acgttggaca
     5281 gcactaccgt gtggaactgt gccgccagct caagatagtc attctgcgag cgcgggccgc
     5341 cgcacagcac ggcgaagtcg aaccagacca cgccgcccgc ccgccgccgc gcgcggatcg
     5401 tccgcgactc gatgatcagc accggatcat cgtcctcggc ttccgcaacg cgatcgaatg
     5461 cggcagccag cgcctgctcg gccgcggcgc cccagggagt gagataggcc tcgatgcgtt
     5521 ccatcgcgcg ccgccggtag tccacgccac catcgacatg cacgacctcg agccggtcct
     5581 tgagcagatc gatcgcaggc agcatgcggt cgcgatgcag cccatcctgg tacagggcat
     5641 ccggtacgta gttcgacgtg atgacgaagc cgacgttcag tgcaaaaagg cgatcgagca
     5701 agcgatacag gatcatcgcg tctgcgatgt ccgacacgtg aaactcgtcg aagcagatga
     5761 gtcgatgccg ccgcgcgatt cgctgcgcga cttcgtctag cggctcggcc gtgcccttta
     5821 gctcgtgcag ttccgcgtgg acgccgcgca tgaactcgtg gaagtgcacc cgcgccttgc
     5881 ggatcaccgg cacagtggcg aagaacgcat ccatcaggaa gctcttgccg cggccgacat
     5941 cgccccacag gtagacgccg cgcggcaccg gtggacgaac cagcagcttt ctcagcgcgt
     6001 tcgagcgctg cgacttgtag tcggtccagt cctggtggag acgctgcagt cgttcggcgg
     6061 cccgctcctg tgaatcatcc agtgcgtatc cgcgttgagc gagcgcggcc tcgagctggg
     6121 gtcgaaacga cacgcgggcg ttgtctcagg gaggacgtct tgcagccgac tgcgaggtgc
     6181 tcagaggttg agcgtccgct tgtcgaccgc aagcgccgcc tccttggtgg cctcggatag
     6241 cgacggatgg gcgtggcaga tacgtgcgat gtcctcggcc gaggcgcgga actccatggc
     6301 cacgaccgcc tcggcgatca gctccgacgc gaagggaccg atgatgtgaa cgccgaggat
     6361 ttcatcggtc gccgcgtccg caaggaactt gaccatgccg ttggtgtcgc ccagtgcgcg
     6421 cgcgcgaccg ttcgccgcga atggaaacgt gccggccttg taggcgcggc ccgcagcctt
     6481 cagctgctgt tccgtctggc cgacccacgc gatctctggt gacgtgtaga tcacccaggg
     6541 gatcgtgttg aagttgacat ggccgtgctg gccggcgatg cgctccgcga cagccacccc
     6601 ctcctcttca gccttgtgag ccagcatcgg cccgcgcacg acgtcgccga ccgcccagac
     6661 gttcgggaca ttggtccggc aatcgtcatc gacggcgacg aagccgcgcg catcgagcgc
     6721 cagtccgacc gcctcgacgt tgagcccggt cgtgttcggg acgcggccga tcgagacgat
     6781 cagcctgtcc acggccagtg actgcacgtc acccttggca tccgcatagg tgacgcgcac
     6841 ttcctgggac ttgcccttct tctcgacggc gacggcatcg atcctgacgc cgaggtggac
     6901 cttcagcccc tggcgcgcga agagcttcgc ggcctccttc gcaatctgat cgtcgacaga
     6961 gcccagaaac gccggcagcg cttcgaggat cgtgacctcc gcgccaagcc ggcgccacac
     7021 cgagcccatc tcgagcccga tcacgcccgc gcctatgacg cccaggcgct tcggcacctc
     7081 agggatcgcc agcgcgccat cgttcgacaa tatgcggctc tcgtcgaagt ccgcgcccgg
     7141 cagagggcgc gcattcgaac cggtcgccac gatcacatgt cgcgcgacga ggctctcgct
     7201 cgtcgcggcg tcgacgttca cttcgatgcc cgcgtcgctg cgtccgacga acgagccgcg
     7261 cccgtggaag aacgcaatct tgttcttctt gaacaggaac aggatgccgt cgttgttctg
     7321 cctgacgacg gtgtccttgc ggccgagcat cttgccgagg tccagaccaa gccccttgac
     7381 ctcgatgccg tgagccgcga agtggtggcc ggcgtgctca tagtgctccg aggattgcag
     7441 cagcgccttg gacggaatgc agccgatatt ggtacacgtg ccgccggggg ccggcccgcc
     7501 gtccttgttc ttccactggt cgatgcaggc gaccgaaaac ccgagctgtg ctgcccggat
     7561 cgccgctatg taaccacccg gtccgccgcc gatcacgacg acgtcgaagt tcttcgacat
     7621 gcttgccttt caggacgtga cgccgagctc agtactcgcg cggcacgaat atccacagca
     7681 ggacgtagag gaccagcgtg acgccgccga ggaacaggcc caacgcaaac agcagccgcc
     7741 agatccagga gtcgactccc gtcgtcttgc cgagtccacc gcagacgcct gcgatccact
     7801 tgtcggagtc cgacaggcgc aggcggttgg caccttccgc ggccgccttc agagggtcgc
     7861 cggtgctgtc tgtggactgg cggcggaaca actcggcctt ggccgtctcg aactcggcct
     7921 gggtcagtgc cccgtccttg tacagcgcat gcagtttccc gatctcgtca gccgtgccca
     7981 ttgtctagac ctccagtagc aggcgcgccg gatcctccag cgcttccttc atcgtgacca
     8041 gtgacagcac tgcctcgcga ccatcga
//
LOCUS       DATIRT010000519         5956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_879850, whole genome shotgun sequence.
ACCESSION   DATIRT010000519 DATIRT010000000
VERSION     DATIRT010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5956
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879850"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="VLN25_11915"
     CDS             <1..1085
                     /locus_tag="VLN25_11915"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HSQ11298.1"
                     /translation="ASGRRGDRESPARLEMRSASAQHRGMLAAFFGTAFLVFFALSPL
                     RWVELGMPWLTLVITFVLIPLLDAAVGRPSPTAQRNGPAFARWIPRLQLPLQAVLLVQ
                     AVLIAPTLDPWQFVLFGLAVGTVTGGLGITIAHELGHRAAQIDRVIAKALLVSVCYGH
                     FFVEHVRGHHVRVATPDDPASAPRGMNVYRFIVRSVVGSLLHAWRLEALRLAQSGRGP
                     WHQSNWVLTGSLMSLALMATAAGLGGAPGLLLFALQAAVAVALLEIINYIEHYGLQRR
                     RIEGRYEPVRELHSWNADFIVSNWILFNLQLHSDHHAHMRRPYEQLCTVHDAPQLPAG
                     YPAMVVLALVPPLWFAAMDPRLPVAA"
     gene            1105..1842
                     /locus_tag="VLN25_11920"
     CDS             1105..1842
                     /locus_tag="VLN25_11920"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HSQ11299.1"
                     /translation="MKLEPIEEAHGWMRDARRITVLTGAGMSAESGVPTFRDAQTGLW
                     SQFDPMQLASEPGFRADPALAWRWYAGRRDAVARAEPNAGHAALAMAEQRYDRCSIAT
                     QNVDGLHRRAGSRNVIEIHGSITQTICLAKCGFVENDPGRLPRGEPPRCPRCGDWLRP
                     GVVWFGEMLDPARLAAAETASADCDVMLVVGTSGLVYPAAGLPLSARRAGAQVIVVNP
                     SDSELDPLARIVVRATAAAALPRLLAR"
     gene            2016..2399
                     /gene="rpsF"
                     /locus_tag="VLN25_11925"
     CDS             2016..2399
                     /gene="rpsF"
                     /locus_tag="VLN25_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008811636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HSQ11300.1"
                     /translation="MRHYEICFIVHPDQSEQVPAMIDRYKAMITAGNGALHRIEDWGR
                     RQLAYPIEKLVKAHYVLMNIECDNKTLDELEHAFRYNDAVLRHLTIKMKKAETSASPM
                     MKVVEREEARKAAAEQSAAAVPVAA"
     gene            2482..2772
                     /locus_tag="VLN25_11930"
     CDS             2482..2772
                     /locus_tag="VLN25_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008543738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HSQ11301.1"
                     /translation="MNELRISATPVARSALRYTPAGVAVAEGSFRHGGALIEAGTERT
                     LDFEFAAIAVGPVAIGLDREPLGQPLTLSGFIAPKTRRSNRLIVHITEYARQ"
     gene            2833..3117
                     /gene="rpsR"
                     /locus_tag="VLN25_11935"
     CDS             2833..3117
                     /gene="rpsR"
                     /locus_tag="VLN25_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HSQ11302.1"
                     /translation="MRGKGKGKGKNDKGKRRPSQQNALFKRKKFCRFTAEKVDQIDYK
                     DVDTLRDFVGENGKITPARLTGTKAHYQRQLDTAIKRARFLALLPYTDTH"
     gene            3133..3585
                     /gene="rplI"
                     /locus_tag="VLN25_11940"
     CDS             3133..3585
                     /gene="rplI"
                     /locus_tag="VLN25_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HSQ11303.1"
                     /translation="MQVILLEKVVNLGQLGEVVKVKEGYARNFLIPQGKARRATETAI
                     KEFETRRADLEKAQADKLAAAQAVQARLSGTKVQVTAKAGVDGRLFGSITNYDIAEAL
                     TKQGFEVNKAQVRMPQGPLKLVGDHPITVALHSDVTAEINVTVVGEQA"
     gene            3744..5123
                     /locus_tag="VLN25_11945"
     CDS             3744..5123
                     /locus_tag="VLN25_11945"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HSQ11304.1"
                     /translation="MNQPVSDPQLAAVRTPPHSIEAEQSVLGGLLLDNTAWDRIADLV
                     GTDDFYRNDHRLIFAAISRLIDAGKPADAVTVFEALRNSNKADDAGGLVYLNTLAQNT
                     PSAANIRRYAEIVRERSVLRRLVTAGDEIATSALNPAGRDTKQILDDAESRVFRIAEE
                     GARGRQGFIEIQPLLTKVVERIQELFERANPSDVTGAPSGFTDLDSKTSGFQPGDLII
                     IAGRPSMGKTALALNIGEHVAIDQGMPVAVFSMEMGASQLALRMLSSVGRLDQQRLRT
                     GRLLDDDWPRLTTAIQKMHEAPLYIDETPALTAIDLRARARRLSRTCGKLGLIIVDYL
                     QLMSASGQGENRATEISEISRGLKALAKELAVPVVALSQLNRTVEQRTDKRPVMSDLR
                     ESGAIEQDADVILFIYRDEVYNPDTNDKNIAEIIIGKQRNGPIGRVNLRFDGQFTKFE
                     NLARSAQFA"
     gene            5174..5794
                     /locus_tag="VLN25_11950"
     CDS             5174..5794
                     /locus_tag="VLN25_11950"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HSQ11305.1"
                     /translation="MLRRLIPRDRRFYPLFNRQAQLCIEGLTALSELLANVRDPHGRV
                     RDVEAIEKRGDGVVAETLALLHRSWLPPFPRLAIHALINRLDDILDLDEDVAQSLHLY
                     HVTAMTAEGRRLAELAVQSAGALERAVAGLEAPGDGSVVLALCEEVNSLEAQADHVFR
                     AAMSRLFREEPDTRQLVKLKAIYELLEELTDKCKNAAAEVQALALR"
BASE COUNT         1082 a         1905 c         1940 g         1029 t
ORIGIN      
        1 gcgccagcgg acggcgcggg gatcgcgagt ctccggcccg gcttgaaatg cgttcggcaa
       61 gcgcgcagca tcgcggcatg ctggccgcct tcttcggaac cgcattcctg gtcttcttcg
      121 cgctcagtcc gctgcgctgg gtcgaactcg gcatgccgtg gctcacgctc gtgatcacct
      181 ttgtgctgat accgctgctc gatgccgcag tcgggcgacc ttcaccaacg gcgcaacgca
      241 acggacctgc gtttgcgcgc tggatcccgc ggctgcagtt gccgctgcag gccgtgttgc
      301 tggtgcaggc cgtgctgatc gcgccgacgc tcgatccctg gcagttcgtc cttttcggct
      361 tggccgtcgg aacggtcacc gggggtctcg gcatcacgat cgcgcatgaa ctggggcacc
      421 gcgccgcaca gatcgaccgc gtgatcgcca aggcgttgct ggtcagcgtg tgctacggac
      481 acttcttcgt cgagcatgtt cgcggtcacc acgtgcgggt cgcgacaccg gacgatccgg
      541 cctcggcgcc gcgcggcatg aacgtctatc gctttatcgt gcgttccgtg gtcggcagtc
      601 tgttgcatgc ctggcggctc gaggcgttgc ggcttgcgca aagcgggcgg ggtccatggc
      661 atcagtccaa ctgggtgctc accggcagcc tgatgtcttt ggcgctgatg gcaacggcag
      721 ccgggctcgg tggcgcacct gggctgctgc tgttcgcgct gcaggcggcc gtcgcggtcg
      781 cgctgctcga gatcatcaac tacatcgagc actacggcct ccagcggcgt cggatcgaag
      841 gtcgctacga accggtgcgc gagctgcatt cgtggaacgc cgatttcatc gtcagcaact
      901 ggatcctgtt caacctgcaa ctgcactccg accatcatgc ccacatgcgg cgaccttatg
      961 agcagctgtg cacggtgcac gatgcgccgc agttgccggc cgggtacccc gccatggtgg
     1021 tcctggcgct ggtcccgccc ctgtggtttg ccgcaatgga cccgcgcctg ccggtggcgg
     1081 cctaggcgat ccatagggcg ttgggtgaag ctcgagccga tcgaggaagc gcatggttgg
     1141 atgcgtgacg cccgtcgcat caccgttctg acgggcgccg ggatgtctgc ggaaagcggc
     1201 gtgccgacgt ttcgcgacgc ccagaccggc ctctggtcgc agttcgatcc catgcagctg
     1261 gcctcggaac cggggttccg agccgatccg gccctggctt ggcgctggta tgccggacgc
     1321 cgcgatgcgg tcgcgcgcgc cgagccgaat gccggacatg cggccctggc gatggcggag
     1381 cagcgttacg accgatgctc gatcgctacg cagaacgtcg acgggctgca ccgacgtgcc
     1441 ggatcacgca acgtcatcga gattcatggc tcgatcacgc agacgatctg cctcgcgaaa
     1501 tgcggctttg tcgagaatga tccggggcgc ttgccgaggg gcgagccgcc acgatgcccc
     1561 cgctgtggcg actggttgcg ccccggcgtc gtctggttcg gagaaatgct cgatccggct
     1621 cgactggcgg cggccgagac tgcatcggcg gactgcgacg tgatgctcgt cgtcggcaca
     1681 tcgggtctcg tttacccggc ggcggggcta cccctgtcgg cgcggcgcgc aggtgcccag
     1741 gtcatcgtcg tcaacccgtc ggacagcgaa ctcgacccgt tggcccgaat tgtcgtccgg
     1801 gcgacggcgg cagcggcgtt gccacgcctg ctggcgcgct gactctggcc ttgtgcggcg
     1861 cggcgcaatt cggctagaat cgcaggcttt tcaagccttt gcgggctacc ttgctgcacc
     1921 ggaacgacgc tccgggcagc tcaccttccg gcatcgctag agcaatgcgc atgcgcagtg
     1981 cgccgacgat gccgggccat ccacaaggag caacgatgcg tcattatgaa atctgtttca
     2041 ttgtgcatcc cgatcagagc gagcaggtgc ccgcgatgat cgatcgctac aaggcgatga
     2101 tcacggccgg taacggcgcg ctgcatcgga tcgaggactg gggccgtcga cagctggcct
     2161 atccgatcga aaaactcgtg aaagcgcact acgtgctcat gaacatcgag tgcgacaaca
     2221 agacgctcga tgagctcgag catgccttcc gctacaacga cgccgtgctg cgccatctca
     2281 ccatcaagat gaagaaggcc gaaacatcgg cctcgccgat gatgaaagtg gtcgagcgcg
     2341 aagaagcacg caaggccgcc gccgaacaat cggcagcggc ggtaccggtc gcggcctgag
     2401 caggccgaag tcggtcggcg atcctcgcgg acgttgacgt gacgtcggtg cagaggccag
     2461 cggacagcgg caagagcagc ggtgaacgaa ctgcgcatat ccgccacacc ggtcgcccgt
     2521 agcgcgctgc gctacacgcc ggccggagtg gcagtggcag agggcagttt ccgccacggg
     2581 ggtgcgttga tcgaggcagg taccgaacgg acgctcgact tcgagtttgc cgcgatcgcc
     2641 gtgggacccg tggccatcgg actcgatcgg gaaccgctcg ggcaaccgct gaccctctca
     2701 ggcttcatcg ccccgaaaac gcgtcgttcg aaccgattga tcgtgcacat caccgaatac
     2761 gccagacagt aaagattttg tcggaaccat cacaggaatt caggagcaca ggagcaccac
     2821 atggcaacct cgatgcgagg caagggcaaa ggcaagggca agaatgacaa gggcaagcgg
     2881 cgcccgtcgc agcaaaatgc gctcttcaag cgcaagaagt tctgccgctt cacggccgag
     2941 aaggtcgacc agatcgatta caaggacgtc gatacgctgc gggatttcgt cggcgagaac
     3001 ggcaagatca ctccggcccg actcaccggc accaaggcgc actatcagcg ccagctcgac
     3061 acggcgatca agcgtgcgcg tttccttgcg ctgttgccgt ataccgatac ccattaagcg
     3121 aggtcgaccc acatgcaagt catactgctc gaaaaggtcg tcaacctcgg ccagctgggt
     3181 gaagtcgtca aggtcaagga aggctatgcg cgcaacttcc tgattccgca gggcaaggca
     3241 cgccgcgcga ccgaaaccgc gatcaaggaa ttcgaaaccc gccgtgcgga tctcgagaaa
     3301 gcgcaggccg acaagctggc cgccgcccag gcagtgcagg cgcgactgag cgggaccaag
     3361 gtccaggtga cagctaaggc cggagtcgat ggccgcttgt tcggctcgat caccaactac
     3421 gatatcgccg aagcgctgac gaagcagggc ttcgaggtca acaaggcgca ggtccggatg
     3481 ccgcagggcc cgctcaaact ggtgggcgat cacccgatta ctgttgccct gcattcggat
     3541 gtgacggcag agatcaacgt gacggtcgtg ggtgaacagg cctgagactg caaagttgcg
     3601 ccaactcagg gcggccgcac ggccgccctt tttattccat gacgacccgc cgttgatgcc
     3661 ggcgcccatg ccgccgttgc tgtagtgttc gcactgcgcg gacgaccgtt cgcgatcgtt
     3721 tccctcttcg cattctgcgt tccgtgaacc agccggtttc tgatcctcag ctcgccgcgg
     3781 tgcgtacacc gccgcattcc atcgaggccg aacagtcggt gctcggcgga ctgctgctgg
     3841 acaacaccgc ctgggaccgg atcgccgacc tggtcggcac cgacgacttc taccgcaacg
     3901 accatcgact catcttcgcg gcgatcagcc gcctgatcga cgccggtaag ccggccgacg
     3961 cagtcacggt gttcgaggcc ttgcgcaact cgaacaaggc cgacgatgcc ggcggcctcg
     4021 tctatctcaa cacgttggcg cagaacacgc cgtcggcggc caacatccgc cgttatgccg
     4081 agatcgtgcg cgaacgctca gtcctgcgac gcctggtcac ggcgggtgac gagatcgcca
     4141 ccagcgccct gaatcctgcg ggacgcgaca ccaagcagat tctggacgat gcggagagcc
     4201 gggtgttccg catcgccgag gaaggcgcga gaggccggca gggtttcatc gagatccagc
     4261 cgctgctgac gaaggtggtt gagcggatcc aggaactctt cgaacgcgcc aatccctcgg
     4321 acgtcaccgg agcaccgagc ggattcacgg atctcgactc caagacgtcg ggattccagc
     4381 caggcgatct gatcatcatc gcgggccgcc cgtcgatggg caagacggca ctcgcgctca
     4441 acatcggcga gcacgtcgcg atcgatcagg gcatgccggt ggcggtgttc tcgatggaaa
     4501 tgggagcgtc gcagctcgcg ctgcgaatgt tgagctccgt tggccggctc gaccagcagc
     4561 gactgcgaac cgggcggctc ctcgacgacg actggcctcg cctgacgacc gccatccaga
     4621 agatgcacga ggcgccactg tacatcgacg aaacgccggc gctgaccgcc atcgacttgc
     4681 gcgcgcgtgc gcggcggctg tcgcgcacct gcggcaagct cgggctcatc atcgtcgatt
     4741 acctgcagtt gatgagtgcg agcgggcagg gcgagaaccg agcgaccgaa atctccgaga
     4801 tctcgcgcgg cctgaaggcg ctggccaagg aactcgctgt tccggtggtt gcgctgtcgc
     4861 agctgaaccg caccgtggag cagcgcaccg acaagcgacc ggtgatgtcg gacctgcgcg
     4921 aatcgggggc gatcgagcag gacgcggacg tgattctctt tatctaccgc gacgaggtct
     4981 acaacccgga caccaacgac aagaacatcg ccgagatcat catcggcaag cagcgcaacg
     5041 gtccgatcgg gcgcgtgaat ctgcgcttcg acgggcagtt caccaagttc gagaatctcg
     5101 cgcgcagcgc gcagttcgca tgacatcgcg gtttgtcccg gatcgaccgg aacgccgcgg
     5161 gttgaggggc tcgatgctgc gtcgactcat accccgcgat cgccgcttct acccgttgtt
     5221 caaccgccag gcgcagttgt gcatcgaggg tctgacagct ctgtcggaat tgctggccaa
     5281 cgtgcgtgac ccccacggcc gggtgcgaga cgttgaggcc atcgagaagc gcggcgacgg
     5341 ggtcgtggcc gagaccctgg cattgctgca ccgctcgtgg ctgccgccgt ttccccgtct
     5401 ggcgatccac gcactcatca accgcctgga cgacatcctg gacctggacg aagatgtcgc
     5461 gcagtcgctg catctgtatc acgtgacagc aatgacagcc gagggccgcc ggctggccga
     5521 acttgcggtg caaagcgccg gcgcgctcga gcgcgccgtg gccggcctgg aagcgccggg
     5581 cgacggtagc gtggtcctcg cgctgtgcga ggaagtgaac tcccttgaag cgcaggccga
     5641 tcatgtgttc cgtgccgcca tgtcgcggct ttttcgtgaa gagcccgaca cacggcagct
     5701 cgtcaagctc aaggcgatct acgagttgct cgaagaactc acggacaagt gcaagaacgc
     5761 ggcagccgag gtccaggcgc tggcattgcg ttaggacaga ccgaacgtga ctgtcactct
     5821 gtggatactg gctgtcgtgc tgatcgtcgt cggagtggcc ggcacctttt tgccggcgct
     5881 gcccggcatc ccgctgatct tcggcggagt tctgctggcg gcctggatcg atgacttcca
     5941 gcgcattcca ggctgg
//
LOCUS       DATIRT010000520         1976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1175957, whole genome shotgun sequence.
ACCESSION   DATIRT010000520 DATIRT010000000
VERSION     DATIRT010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175957"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /gene="pyrF"
                     /locus_tag="VLN25_11955"
     CDS             <1..633
                     /gene="pyrF"
                     /locus_tag="VLN25_11955"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HSQ11306.1"
                     /translation="AAARAENQLEQLFAHIRREHPQVPVILDAKRGDIGATAEHYARE
                     AFERYGADAVTLSPYMGKDSIDPFLAYADRGAFLLCRTSNVGGDDLQMLDVDGVPLYQ
                     RVAAMAAGEWSTGGQLGLVVGATYPAELARVRSLVGDMPLLVPGIGVQGGDVAASVAA
                     GQTADGTGMVINSSRAILYAGDGLDYAERASEAARAIRDEINRHRHRASA"
     gene            complement(636..1958)
                     /locus_tag="VLN25_11960"
     CDS             complement(636..1958)
                     /locus_tag="VLN25_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HSQ11307.1"
                     /translation="MPTEVLLLFGLILLNGVFAMSEIALVTARRARLQALVEQGDAGA
                     AAALALNDEPTRFLSTIQVGITSIGILSGIVGEAAFAEPLSHWLIALGAEPKSAQLGA
                     TALVVVVVTYFSIVLGELVPKRLGQISAEAIARRVARPIAWLALAAKPFVRLLTASTD
                     LMLRVIGARSDGSSAVTEEEIHALIKEGSETGVIDEQERAMVRNVFRLDDRQIASLMT
                     PRSEIVYLDLEDDLKDNLHRVLDIEHSRFPVCRGGLRDVVGVASARRLLQQAIRGDVL
                     NLERAAETVVYVPESLTGMELLENFRASAGHMALVVDEYGEIQGLVTSQDLFEAIAGE
                     FKPQRTEDAWAVQRADGSWLLDGLIPIPELKDRLNFGAAPEEELGRYNTLSGMVMLLL
                     GRVPATGDVVEWHGWRFEIVDMDKHRIDKVLAAQTARVADMANGAAGK"
BASE COUNT          333 a          689 c          640 g          314 t
ORIGIN      
        1 gccgccgcgc gcgccgagaa ccagctcgag cagctgttcg cgcacatccg ccgcgaacat
       61 ccgcaggtgc cggtgatcct cgacgccaag cgcggcgaca tcggggccac cgccgaacac
      121 tatgcgcgcg aggcgttcga acgctacggc gcggacgcgg tcacgctgtc gccctacatg
      181 ggcaaggatt cgatcgaccc ctttcttgcg tacgccgacc gcggcgcctt cctgctctgt
      241 cgtacgagca acgtgggcgg cgacgatctg cagatgctcg atgttgacgg agtgcctctc
      301 taccagcgcg tggcggcgat ggcagcgggc gagtggagca ccggcggcca gctgggcctg
      361 gttgtcggcg cgacctatcc ggccgagctg gcgcgggttc gttcgctcgt gggtgacatg
      421 ccgctgctgg tgcccggcat cggcgtgcag gggggcgatg tcgctgccag tgtcgctgcc
      481 ggccagacgg cggacggcac cggcatggtg atcaactcgt cgcgtgcgat cctgtatgca
      541 ggcgacgggc tcgactacgc cgagcgcgca agcgaggcgg cgcgcgccat ccgcgacgag
      601 atcaaccgcc accgtcaccg cgcaagcgcc tgacctcact tgccggcggc accattggcc
      661 atgtcggcca cgcgcgccgt ctgcgcggcc agcaccttgt cgatacggtg cttgtccatg
      721 tcgacgatct cgaagcgcca gccgtgccac tcgacgacgt cgccggtggc cggcaccctg
      781 ccgagcagca gcatcaccat gccggagagc gtgttgtagc gtcccagttc ctcctcgggc
      841 gcggcgccga agttcaagcg gtccttgagt tcgggaatcg gaatcagtcc atcgagcagc
      901 cacgagccgt cggcgcgctg caccgcccag gcatcctcgg tgcgttgcgg cttgaactcg
      961 ccggcgatcg cctcgaacag gtcctgcgag gtcacgagtc cctggatctc gccgtactcg
     1021 tcgaccacga gcgccatgtg cccggcgctg gcccggaaat tctccagcag ttccatgccc
     1081 gtcagcgact cgggcacgta gaccaccgtc tcggccgcgc gctcgaggtt gagaacatcg
     1141 ccgcggatgg cctgctgcag cagccggcgc gcactcgcca cgccgacaac gtcgcgcagg
     1201 ccgccacggc acaccggaaa acgcgagtgc tcgatgtcga gtacccggtg caggttgtcc
     1261 ttcaggtcat cctcgagatc caggtacacg atctccgaac gtggcgtcat caacgatgcg
     1321 atctgacggt cgtcgagccg gaacacgttg cgcaccatcg cgcgttcctg ctcgtcgatc
     1381 acaccggttt ccgagccttc cttgatcagc gcgtggatct cctcctcggt gacggcgctg
     1441 gatccgtccg aacgcgcgcc gatcacccga agcatcaggt cggtggacgc cgtcagcagc
     1501 cgtacgaacg gcttcgcggc cagcgccagc caggcgatcg gtcgcgccac ccgacgcgca
     1561 atcgcctcgg ccgagatctg acccaggcgc ttcggcacca gttcaccgag cacgatcgag
     1621 aagtacgtga cgacgacgac gaccagcgcc gtcgcgccga gctgagcact tttcggctcg
     1681 gcgcccagcg caatcagcca atgcgaaagg ggttcggcga aggcggcctc gcccacgatc
     1741 ccgctcagga tgccgatcga tgtaataccg acctgaatcg tcgacagaaa ccgcgtaggc
     1801 tcgtcgttca gcgccagtgc ggccgccgct ccggcatcgc cctgttcaac cagcgcctgc
     1861 aggcgggctc ggcgcgcggt cacgagcgcg atttcggaca tggcgaatac gccgttgagc
     1921 agtatcaatc caaacaacag caggacttcg gtcggcatgg caatccgtgg tgagag
//
LOCUS       DATIRT010000521         4051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_93392, whole genome shotgun sequence.
ACCESSION   DATIRT010000521 DATIRT010000000
VERSION     DATIRT010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93392"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /locus_tag="VLN25_11965"
     CDS             <1..1307
                     /locus_tag="VLN25_11965"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HSQ11308.1"
                     /translation="AVVEAPLVAGVVSPAAMSSPASAPAAAPTMSLIAALGAAFVGGL
                     ILNLMPCVFPVLSLKLIGLAQHRTHSGPMAAHGAAFAAGVILSFVLLAGLLIALQQAG
                     SALGWGFQLQTPWVVTLLTILFFVIGLNLLGVYEFTFGSGLGNTKAADALVGKSDWRG
                     SFGTGVLAVIVASPCTAPFMSAALGYAITQPAAIALSVFAMLGVGMAAPYLLLTLFPA
                     LLAKLPRPGRWMELFKQFMAFPMFATCVWLLWVLAQQVDAGGVALALGVLVAVSFALW
                     SLGLAQRGASRFRWVALAVAVLAAITFTPIATSEPVANGGRASISADWVEYSPQKLAE
                     LRGQGKAVFVDFTAAWCVTCQVNKRVALNTDKVKSRFADSDIVRMKADWTNRDEQITR
                     ALAEFGRNGVPLYVLYDRTGKPTVLPELLTEKTVLAALDKLN"
     gene            1318..1920
                     /locus_tag="VLN25_11970"
     CDS             1318..1920
                     /locus_tag="VLN25_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HSQ11309.1"
                     /translation="MKHAASVILSSCLLAAGSAVAAVTAGQPAPAFKGTDVSGKPVTL
                     ADFKGKYVVLEWNNPNCPFVMKHYDSGNMQALQKRYGADNVAWISVNSTSDSHSDYMS
                     PERLAAWFKQQNAAPAAVLMDTKGEIGRAYGAKVTPHMYIVDPKGTVIYAGAIDDKRS
                     ANPADVKSATNYVTAAMTDARSGKPVATASSSAYGCTIKY"
     gene            complement(2013..3659)
                     /gene="groL"
                     /locus_tag="VLN25_11975"
     CDS             complement(2013..3659)
                     /gene="groL"
                     /locus_tag="VLN25_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828028.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HSQ11310.1"
                     /translation="MASKQVFFGDDARHRMVKGVNILANAVKVTLGPKGRNVVLERSF
                     GAPTVTKDGVSVAKEIELKDKFENMGAMMVREVASKTSDNAGDGTTTATVLAQSIVAE
                     GMKYVAAGMNPMDLKRGIDKAVIAAVEELKKISKPTTTSKEIAQVGSISANADPEIGK
                     IIADAMDKVGKEGVITVEDGKSLNTELEVVEGMQFDRGYLSPYFINQPDKQIAILENP
                     YVLLHDKKISNIRDLLPILEQVAKAGRPLLIVAEEVEGEALATLVVNSIRGILKTCAV
                     KAPGFGDRRKAMLEDIATLTGGTVIAEETGMTLEKATLNDLGQAKKVEIGKEDTTIID
                     GAGETKTIEARVKAIRAQIEEATSDYDKEKLQERVAKLAGGVALIKVGAATEVEMKER
                     KARVEDALHATRAAVEEGIVAGGGVALIRAKQAISKLKGENTDQDAGIKIVLRAMEEP
                     LRQIVANSGDEPSVVVNAVANGKGNYGYNAATHEYGDLVEMGVLDPTKVTRTALQNAA
                     SVASLMLTTDAMVAELVEDKPAAHGGGGGMGGMGGMGMDM"
     gene            complement(3718..4005)
                     /gene="groES"
                     /locus_tag="VLN25_11980"
     CDS             complement(3718..4005)
                     /gene="groES"
                     /locus_tag="VLN25_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HSQ11311.1"
                     /translation="MKIRPLHDRVIVKRLDQERKTASGIVIPDNAAEKPDQGEIMAVG
                     PGKRDDNGKIVPLDVKVGDKVLFGKYSGQTVKVEGEELLVMREEDLMGVVA"
BASE COUNT          646 a         1313 c         1242 g          850 t
ORIGIN      
        1 cggccgtcgt cgaagcacct ctcgtggccg gagtggtcag tcccgccgct atgtcgtcgc
       61 ccgcctcagc gccggcagcc gctcccacga tgtcgctgat cgcggcgctg ggcgcggcgt
      121 tcgtcggcgg cttgatcctg aacttgatgc cgtgcgtgtt tccggtcctg tcgctgaaac
      181 tgattggcct ggcacagcac cgcacgcaca gcggaccgat ggctgcgcac ggtgctgcat
      241 tcgccgctgg cgttattctt tcgttcgtgc tgctggcggg gctgctgatc gccctgcagc
      301 aggccggcag cgcgctcggg tggggcttcc agttgcagac gccgtgggtt gtaacgctgc
      361 taaccatcct gtttttcgtg atcgggctca acctgctggg cgtgtacgag ttcacgttcg
      421 gcagcggtct tggcaatacc aaggcggccg atgcgctggt gggcaagtcc gactggcgcg
      481 gcagcttcgg caccggcgta ctggctgtga tcgtcgcgtc gccctgcaca gcgccgttca
      541 tgagcgcggc cctcggctac gcgatcacac agccggccgc catcgccctg tccgtgttcg
      601 caatgctcgg ggtcggcatg gcagcgccct acctgctgct gacactgttc ccggcgctgc
      661 tggcaaagct gccgcggccg ggtcgctgga tggaactgtt caagcagttc atggcgttcc
      721 cgatgtttgc cacctgcgtc tggttgctgt gggtgctggc acagcaggtc gatgccggcg
      781 gcgtggcgct cgcccttggc gtgctcgtgg cggtctcgtt tgcgctctgg tcgctgggac
      841 tggcgcagcg cggagccagt cgattccgct gggtcgcgct ggccgttgcc gtgctcgccg
      901 cgatcacctt cacgccgatc gccacatcgg agccggtcgc caacggcggc cgggccagca
      961 tcagtgccga ctgggtcgaa tactcgccgc agaagctggc cgagctgcgc ggccaaggca
     1021 aagccgtgtt cgtcgacttc acggccgcct ggtgcgtgac ctgccaggtc aacaagcgcg
     1081 tggcgctgaa caccgacaag gtcaagtcgc gcttcgctga ctccgatatc gtgcgtatga
     1141 aggccgactg gaccaatcgt gacgagcaga tcacgcgcgc gctggccgaa ttcgggcgca
     1201 acggcgtgcc cctgtatgtc ctttatgacc gtacgggcaa accgacggtc ctgcctgaac
     1261 ttctgacgga gaagacggtc ctagcggcgc tcgacaagct caactgagga gttctgcatg
     1321 aagcacgccg cttcggtcat cctgtcgtcg tgtctgcttg cggccggttc cgccgtagcg
     1381 gccgtcactg cgggacaacc tgcacctgcc ttcaagggca ccgatgtatc cggcaagccc
     1441 gtgacgctcg ccgacttcaa gggcaaatat gtcgttctcg agtggaacaa ccccaattgc
     1501 ccgttcgtga tgaagcacta cgacagcggc aacatgcagg cactgcagaa gcgctacggt
     1561 gctgacaacg tggcgtggat ttccgtgaat tcgaccagcg actcgcattc cgactacatg
     1621 tcgccggaaa ggcttgcggc ctggttcaag cagcagaatg cggcgccggc ggccgtgctg
     1681 atggacacca agggcgaaat cggccgcgcc tacggcgcca aggtcacacc gcacatgtac
     1741 atcgtcgacc cgaagggcac cgtgatctac gccggcgcca tcgacgacaa gcgcagcgcg
     1801 aacccggccg atgtgaagtc agccacgaat tatgtaacgg ccgcgatgac ggatgcccgc
     1861 tcgggcaagc cggtcgccac cgcctcctcg agcgcttacg gctgcacgat caagtactag
     1921 tcccttactt ctcaaataca aacggcccgc aagtgcgggc cgtttgtatt tgatgcggag
     1981 cgaacgctgc gggcggatgc ccgcaggttt ccttacatat ccatgcccat tccgcccatc
     2041 ccacccattc caccaccgcc gccatgcgcc gccggcttgt cttccacgag ctcggcgacc
     2101 atcgcatcgg tcgtcagcat caggctggcc accgaggcgg cattctgcag cgccgtgcgg
     2161 gtcaccttgg tcggatcaag cacgcccatt tcaaccaggt caccgtactc gtgcgtcgcg
     2221 gcgttgtagc cgtagttgcc cttgccgttg gcaacggcat tgacgaccac gctcggctcg
     2281 tcgccggagt tggcgacgat ctggcgcaac ggctcttcca tcgcgcgcag cacgatcttg
     2341 atgccggcgt cctggtcagt gttctcgccc ttcagcttgg agatggcctg ctttgcacgg
     2401 atcagcgcaa cgccgccgcc cgccacgatg ccttcctcaa ccgctgcgcg cgtagcgtgc
     2461 aacgcatctt cgacgcgcgc cttgcgctcc ttcatctcga cttcggtggc agcgccgact
     2521 ttgatcagcg ccacaccgcc ggccagcttg gcgacacgct cctgcagctt ttccttgtcg
     2581 tagtcgctgg tggcttcctc gatctgggcg cgaatcgcct tgacgcgggc ttcgatggtc
     2641 ttggtctcgc cagcgccgtc gatgatcgtc gtgtcttcct tgccgatctc gaccttcttg
     2701 gcctgtccca gatcgttcag ggtggctttc tctagcgtca tgccggtttc ttcggcgatg
     2761 accgttccgc cggtcagggt cgcgatgtct tccagcatgg ccttgcgacg gtcaccaaag
     2821 cccggcgcct tgacggcgca ggtcttcagg atgccgcgga tgctgttcac caccagggtc
     2881 gccagcgctt cgccttcgac ttcttcggcg acgatcagca gcggacggcc ggccttggca
     2941 acctgctcga gaataggcag aaggtcacgg atgttgctga tcttcttgtc gtgcagcagg
     3001 acgtacggat tctcgaggat tgcgatctgc ttgtccggct ggttgatgaa gtacggcgac
     3061 aggtagccgc ggtcgaactg catgccctcg acgacttcga gttcagtgtt gagcgacttg
     3121 ccgtcctcga cggtgatgac accctctttg ccgaccttgt ccatcgcgtc agcgatgatc
     3181 ttgccgatct cggggtccgc attggcggaa atcgacccga cctgagcgat ttccttgctg
     3241 gtcgtggtgg gcttgctgat cttcttgagt tcctcgaccg ctgcgatgac ggccttgtcg
     3301 atgccgcgct tcaggtccat cgggttcatg ccggcggcca cgtacttcat gccctcggcg
     3361 acgatcgact gcgcgagcac ggtggcggtg gtggttccgt cacccgcgtt gtcggaggtc
     3421 ttggatgcaa cctcacggac catcatcgcg cccatgttct cgaacttgtc cttcagttcg
     3481 atctccttgg cgaccgagac accgtccttg gtgacggtcg gggcgccaaa gctgcgctcg
     3541 aggacaacat tgcggccctt cgggcccagc gtgaccttga ctgcattggc caggatgttt
     3601 acgcccttga ccatgcgatg acgtgcgtca tcgccgaaaa acacttgctt ggaagccata
     3661 gaacctctct ctgaaaatta tgtggccgac cgcgcagcga ccggcggcgg tgaacaatca
     3721 ggcgacgacg cccatcaggt cctcttcgcg catgacgagc aactcctcgc cttcgacctt
     3781 gaccgtctgg cccgagtact tgccgaacag aaccttgtca ccgaccttga cgtcgagcgg
     3841 aacgatcttg ccgttgtcgt cgcgcttgcc cggtccgacg gccatgattt cgccctggtc
     3901 aggcttttct gcggcgttgt cggggatgac gatgcccgaa gcggtcttgc gctcctggtc
     3961 gaggcgtttg acgatcacgc gatcgtgcaa aggacgaatc ttcataaagt ctcctggcag
     4021 ttgatctctc gacgagcgag aaacatgaaa t
//
LOCUS       DATIRT010000522         1644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1077949, whole genome shotgun sequence.
ACCESSION   DATIRT010000522 DATIRT010000000
VERSION     DATIRT010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077949"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VLN25_11985"
     CDS             complement(<1..393)
                     /locus_tag="VLN25_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HSQ11312.1"
                     /translation="MQPMPRTQEVERSTSSGYIWIAVGLALIAVGIYLVVRPAGFIGL
                     AIGIALLIIGAFVVAGLYMLQPNEAAILLLFGAYTGTDRTEGLRWANPFYRKQKISLR
                     AHNFNSERLKVNDKRGNPIEIAAAIVWRV"
     gene            complement(517..1029)
                     /locus_tag="VLN25_11990"
     CDS             complement(517..1029)
                     /locus_tag="VLN25_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013951778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HSQ11313.1"
                     /translation="MKRRLYVVLPDMASAIQTANDLLLARIEDRHMHFLGKSGMSFGT
                     LHEASVLQKSDVRHALFLGAGLGALGGMLLGVYLRLTPIGDITFGAGAFMLATLGGMA
                     FGAWTSTMIGLSIPSSDLKPFESDIEAGKILLMVDVPVARVEEIEQMLARRHPEAVDR
                     GIEPSIPAFP"
     gene            1344..>1644
                     /locus_tag="VLN25_11995"
     CDS             1344..>1644
                     /locus_tag="VLN25_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003261357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome B"
                     /protein_id="HSQ11314.1"
                     /translation="MAIAVVLVILIAGSVLFHFLSPWYFTPIASNWSAIDDTISITFW
                     VTGAVFVLVNLFMAYAIYRYRHRTNARAQYEPENKKLEWWLLAVTTVGVAAMLAPG"
BASE COUNT          302 a          505 c          509 g          328 t
ORIGIN      
        1 aacgcgccag acgattgcgg ccgcgatttc aatcggattg ccgcgcttgt cattgacttt
       61 cagccgctcg ctgttgaagt tgtgcgcgcg caggctgatc ttctgcttac ggtagaacgg
      121 gttggcccag cgcagtccct ctgtgcggtc ggtgccggtg tacgcgccga acagcagcag
      181 gatcgcggct tcgttcggct gcagcatgta aaggcccgcc accacgaagg cgccgatgat
      241 cagcagtgcg atgccgatcg ccaggccaat gaatccggcc ggtcggacga cgaggtaaat
      301 gccgaccgcg atgagggcga gcccgacggc gatccatatg tagccgctcg acgtgctgcg
      361 ttcgacttcc tgcgtgcgtg gcatgggctg catggaacgc ctcctatcaa agtgatatca
      421 ctttgataga aaccacgtca ttgtgtcaag gtgtcgggcg acgaacggcg ccccgcgacg
      481 cccctcgggc cgcccgtcca gcagcccgtc agtagttcag ggaaacgcgg gtatcgacgg
      541 ctcaatgccc cggtcgaccg cttccgggtg ccggcgagcg agcatctgct cgatttcctc
      601 gacgcgcgcg acgggaacat ccaccatcag caggatcttg ccggcttcta tatcggactc
      661 gaacggcttg agatccgaac tcggtattga caagccgatc atcgtcgagg tccaggcgcc
      721 gaaggccatt ccacccaggg tcgccagcat gaatgcgccg gcgccgaacg tgatgtcgcc
      781 gattggcgtg agacgcaggt agacgccgag cagcattcca ccgagagcgc cgagccctgc
      841 tcctagaaac aatgcatggc gcacgtcgga cttctgcagg acgctcgctt cgtgcaaggt
      901 gccgaacgac attccgctct tgccgagaaa gtgcatgtgg cggtcttcga tacgggccag
      961 cagcaggtca ttggctgtct ggatcgcact ggccatgtcg ggcagaacca cgtacagacg
     1021 ccgcttcata cggcctcctt gtcgaatgga gtggggcgac tcgtacgcac cgcgccctcg
     1081 ctccttgcgc gtactcttgt tcgcgtgatc agtatgcact gcgaagtcat gctgcggcta
     1141 ctaccctagg cgcccatgca cacgtggcga tgtccgcacg caaatgattc gtgcggtgca
     1201 caacgtgttt ggccatgcgc acagtcagta gggggtttgc aagggccgca tttaccttta
     1261 ccgttcgcgc gatcgtttgc agcaggacgg gtgcacacag tgacgcgcac ccgactttcg
     1321 aaccagtcac gcgaggtgcc cgcatggcga tcgcagttgt tttggtcata ctgatcgccg
     1381 gctcggtgct gtttcacttt ctgagcccgt ggtacttcac gccgatcgcc tccaattgga
     1441 gcgcgatcga cgacacgatc tcgatcacgt tctgggtcac cggcgctgtc ttcgtgctgg
     1501 tcaatctgtt catggcctac gcgatctatc gctatcggca tcgcaccaat gcgcgggcgc
     1561 agtacgaacc cgagaacaag aaactcgagt ggtggctgct ggcggtgacc acggtgggcg
     1621 tggccgcgat gcttgcgccg gggc
//
LOCUS       DATIRT010000523         2845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_93812, whole genome shotgun sequence.
ACCESSION   DATIRT010000523 DATIRT010000000
VERSION     DATIRT010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93812"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VLN25_12000"
     CDS             <1..260
                     /locus_tag="VLN25_12000"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSQ11315.1"
                     /translation="VSGYVGCAHAISHIDFTARLATIDCPTLVMVGADDQGTPVSMAE
                     EIVQAIDGSRLEIIPDAAHLSNIEQPQRFNALLRQFLATCS"
     gene            405..662
                     /locus_tag="VLN25_12005"
     CDS             405..662
                     /locus_tag="VLN25_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3567 domain-containing protein"
                     /protein_id="HSQ11316.1"
                     /translation="MTMIYNSPQYCVFEFEGSGSDDGRATGGYEIMDKQLRREIFLGG
                     QDAENFRRSVQELIEQGPSPDEVDAFLHGFSGLMTQPVVLH"
     gene            816..2504
                     /locus_tag="VLN25_12010"
     CDS             816..2504
                     /locus_tag="VLN25_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013207248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HSQ11317.1"
                     /translation="MGVCHDGHLLGSAVEFDYIIVGGGSGGCVLAARLSEDPATRVVL
                     LEAGGDNGGVFDRVPVGAALFIMNRNARNWAFATTPQPGLGGRVGYQPRGRGLGGSSA
                     INAMVYLRGQREDYDNWATDGARGWSWKEVLPYFLKAENNERGISGWHGTGGPLNVAD
                     LRSPHPFAERFIQAGMQAGLVRNDDFAGETQEGVGFYQVTQKNGERWSVARAYLDPAR
                     SRPNLRIETQAVATRILFEGTRAVGVEFEQSGVRRTLRARREVLLAAGALQSPQLLMV
                     SGVGPGASLQGLGIPVLADLPVGENLQDHLDVIINRRVDNTDLLGLSPTGAWKLLRAI
                     GRWRRERRGVLTSNFAEAGAFVRTLPELTRPDVQLHFVIGMVDNHNRTYHWGHGMSCH
                     TCALRPQSRGRLHLASRDARDAPLIDPGFLSAPEDLETMVRAFRLVRRIFAQPAFAPF
                     GGGDPARELYFSDVRSDDEIRAAIRSRADTIYHPVGTCRMGADARAVVDPELRVRGVQ
                     GLRVVDASVMPTLVSGNTNAPVVMIAERAADLIRQAGVSGASLDAAAVRESALA"
BASE COUNT          472 a          905 c          974 g          494 t
ORIGIN      
        1 cggtgtccgg ctacgtcggc tgtgcccacg cgatctcgca tatcgacttc accgcgcggc
       61 tggcgacgat cgattgcccg acactggtca tggtcggggc agatgaccag ggcacgccgg
      121 tgtcgatggc cgaagaaatc gtccaggcca tcgatggctc acgcctggag atcattccgg
      181 acgcggcgca cctgtcgaat atcgagcagc cgcagcggtt caatgccctg ctgcgtcagt
      241 tcctcgcaac ctgcagttag cggtcacttt catttctcgt cccgtagaga gcggctcgcc
      301 gcacagcgct tgaatcttcg gcgctggtac cgatattgca tgtagatgag tggcggcaag
      361 actgcaagca caccggccgc tcgaaaggga cgcaagccat ggcactgaca atgatctaca
      421 acagcccgca gtactgcgtg ttcgagttcg aaggctcggg cagcgacgac ggccgtgcca
      481 cgggcggcta cgaaatcatg gacaagcaac tgcgtcgcga gatcttcctg ggtggtcagg
      541 atgccgagaa ctttcgtcgg agcgtgcagg aactcatcga acaaggcccg tcgcccgacg
      601 aggtcgatgc ctttctccac ggattcagcg gcttgatgac acagccggtg gtcctgcact
      661 gatcgaacgc aggcgcggtc cgtcggaacc ccgacatcgc gctggaccga attcggggcg
      721 acgggttgcg cctcgcgcct gcggggaatt cgaggacagc ttctagggaa cccgccgtgg
      781 tgacctgcgg ccggcgccac aattcatcag aatgaatggg cgtgtgccat gacggccacc
      841 tgctaggaag cgcagtggag ttcgactaca tcatcgtcgg cggtgggtcc ggtggctgtg
      901 tactggcggc ccgcctgtcc gaagacccgg ccacccgtgt cgtgctgctc gaggcgggcg
      961 gggacaacgg cggagtgttc gaccgcgtgc cggtcggcgc ggcgctcttc atcatgaatc
     1021 gcaatgcgcg caactgggcg ttcgcgacga cgccgcagcc gggcctgggc ggccgggtcg
     1081 gctaccagcc gcgtggtcgc ggtctcggtg ggtcgtcggc aatcaacgcg atggtttacc
     1141 tccgcggaca gcgcgaggat tacgacaact gggccacgga cggcgcgcgc ggttggagct
     1201 ggaaggaggt ccttccgtac ttcctgaagg cagagaacaa cgagcgcgga atcagcggct
     1261 ggcacggcac gggaggcccg ctcaatgtgg ccgacctgcg ctcaccgcat ccgtttgccg
     1321 agcgcttcat ccaggcgggc atgcaggccg ggctggtgcg caacgatgac ttcgccggcg
     1381 agacacagga aggcgtcggc ttttatcagg tgacgcagaa aaatggcgag cgctggtcgg
     1441 tggcgcgcgc ctacctcgac ccggcgcgtt cacggccgaa ccttcgcatc gagacgcagg
     1501 cagtggccac ccgcatcctg ttcgaaggca cgcgtgcggt cggtgtcgag ttcgagcaat
     1561 cgggagtgcg ccggacgctg cgcgcgcgtc gcgaggtgct gctggcagca ggagcactgc
     1621 agtcgccgca gttgctgatg gtctcgggcg tcgggccggg tgcgtcgctg cagggactgg
     1681 gcatcccggt gctcgcggat ctgccggtcg gcgagaacct gcaggaccat cttgacgtca
     1741 tcatcaaccg tcgtgtcgac aacaccgact tgctgggact gtcgccgacg ggcgcctgga
     1801 agttgttgcg cgcaatcggg cgctggcgtc gcgagcgccg cggcgtgctg acctccaact
     1861 ttgccgaagc cggcgcattc gtgcgcacgt tgccggaact gacgcgtccg gatgtgcagt
     1921 tgcacttcgt catcggcatg gtcgacaacc ataatcgcac ctatcactgg ggccacggca
     1981 tgagctgcca cacgtgcgcg ctgcgcccgc agagccgcgg ccgtctgcat cttgcgtcgc
     2041 gcgatgcgcg cgatgcgccg ctgatcgatc cgggcttcct ctccgcgccg gaagaccttg
     2101 aaacgatggt gcgtgcattc agactcgttc gacgcatctt tgcccaaccg gcgttcgcgc
     2161 cgttcggcgg cggtgatcct gcgcgcgagc tgtatttcag cgacgttcgc agcgatgacg
     2221 aaatccgcgc ggcgatccgc tcgcgcgcgg acaccatcta tcacccggtc ggtacctgtc
     2281 gaatgggtgc ggatgcacgg gcggtcgtcg atccggaact ccgggtgcgc ggcgtgcagg
     2341 gactgcgcgt cgtcgacgcg tcggtgatgc cgacactcgt ctccggcaac accaatgcac
     2401 cggtggtgat gatcgcggaa cgggcggccg acctgatccg ccaggctggc gtgtccgggg
     2461 cgagcctgga cgctgcggcc gtgcgcgaat ccgcgctcgc ctgaacccgg ccgacacggt
     2521 gattccgact ctgcgctaca tcagcgcagg gcatgagttc atgcttgccc gcgcacgtat
     2581 gtacgggcca tgggctttag ggcctgttca cgctaaatgc gggagatgcg ttgggccgca
     2641 cggcggatgg atgcgcgaag ggcggcgctg cgcagggtgg ggatcccctg cgcaagccac
     2701 ccgacaaagc agacgccgcc gtgcggccca accctgcggg aaggccagtg gcgggcgcat
     2761 tgcgtcgtta ccgcttgctg attggcatca gccaacggcg ctcgcggcgc ctcgcactgc
     2821 ctcccgccat tggccttcgc actcc
//
LOCUS       DATIRT010000524        13039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1668373, whole genome shotgun sequence.
ACCESSION   DATIRT010000524 DATIRT010000000
VERSION     DATIRT010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13039
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1668373"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VLN25_12015"
     CDS             <1..316
                     /locus_tag="VLN25_12015"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HSQ11318.1"
                     /translation="SDGEITHQIPVDEVVYIQADDKYTCVYSRDGKELAEWLIRIPLS
                     ELSAQLDPTDFAQIHRSVIVNLHSVTGTRRDLTGKLYVRLRDHPRELPVSRQYLHLFQ
                     KM"
     gene            complement(329..1252)
                     /locus_tag="VLN25_12020"
     CDS             complement(329..1252)
                     /locus_tag="VLN25_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSQ11319.1"
                     /translation="MPQIDANGVTLDVEEFGDADAPVVLLIMGLGMPAAAWPDSFIEP
                     LVRSGLRVIRFDNRDCGHSTKLRSAPMPNLQLAIMRALLRLPVRAPYTLDDMAQDAAD
                     LLAALGITRAHVVGASMGGMIAQVFAARHPQRVRTLTSIMSSSGNPNPHVALGKPRAL
                     RAILDRPRSTDDVAQLTDHLMRVFSVIGSPGFPSEPNALREHLERVARRGYYPAGAAR
                     QLLAILASGDRRRLLAQITAPTLVIHGRDDPLVPLAAGLDTAHHIRGAQLKVIAGMGH
                     DFPPALQPQIAAAIVEHIANAAHGIGDRTAA"
     gene            complement(1273..2064)
                     /locus_tag="VLN25_12025"
     CDS             complement(1273..2064)
                     /locus_tag="VLN25_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSQ11320.1"
                     /translation="MDNSQHWYDFDMMRAPGFVRLALEWRAPWEYGATMLASPWLDQA
                     ADGDGHPVLVFPGLMASDASTKPLRKFLERKGYLAYGWEHGSNLGPKDGVLDHCMQRV
                     QKLRRQHRRKVSLIGWSLGGIYARELAKDFPRDVRQVITLGTPFAGHPKATNAWRLYE
                     LVTGHRIGAPEIHEPLRQAPPVPTTSIYSRTDGVVAWQCSVERRSAHAENIEVEASHF
                     GIGLNALAWYAIADRLAQPEGQWQPFDRSGPLRWLYRDPTRKAWF"
     gene            complement(2115..3557)
                     /locus_tag="VLN25_12030"
     CDS             complement(2115..3557)
                     /locus_tag="VLN25_12030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HSQ11321.1"
                     /translation="MKPLSGLDSLFLHLETPATPMHVGAVHLLERPRRGGDYLLRVRR
                     HVARRVHLSPVFTRQLAHMPLDFANPVWVRAEQVDLERHVVRVTLPAPGTLAQLEAAV
                     ARLHAQPLDRSRPLWRFHVIEGLQDGGLGFYTKIHHATLDGASGVALAHALLDLAPSP
                     RAVEPARKRRRDQPGLVMMVGTALKVSGSQTVDLVRRLPALARVAARLVTGSTGFSRN
                     LAFGPRTAFNRVIDSGRSFATASLPLADVRAIAAAHEATLNDVVLTLVSGALRGYLKA
                     RGGVPTKSLVAAMPVSLREAGNTEATTLATMTLASLATTLADPIERLRAVQASTRAAK
                     DVTRQIKGVIPTDFPSLGLPWLLSAAAALYGRTSLADRIPPIANLVVSNVPGPDAPLY
                     LAGARLKTYWPVSIVEHGLGLNITLQSYAGSLDFGILAARVGIPDARPLARGLHEALA
                     ELRRATDIAASPRVEPDTAKAPPRRRAAAR"
     gene            3657..4649
                     /gene="fmt"
                     /locus_tag="VLN25_12035"
     CDS             3657..4649
                     /gene="fmt"
                     /locus_tag="VLN25_12035"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HSQ11322.1"
                     /translation="MRIVFAGTPDFAARSLAALIAAAPAAGWSLPLVLTQPDRPAGRG
                     LRLTPSPVKSLALAHGLTVDTPVSLRKGPEAEAAQRWIAAVEPDVLIVAAYGLILPQS
                     VLDIPRGLRPGWSPKITAINVHASLLPRWRGAAPIARAIEAGDSVTGITIMQMDAGLD
                     TGPMLLAESIDIDADETAGQLTARLADLGATLLVRALVSIAQLSAQPQSAAGATYAAK
                     IGKAEAWLDWSLPAAVLARKVRAYDPFPVAHARIGEASIKVWRAIAVPATAEVAPGTI
                     VAAGAAGIDVRCGHGCLRLTELQRPGGKRLAAREFLAGHALAPLQCFDVGPAHG"
     gene            4642..5976
                     /gene="rsmB"
                     /locus_tag="VLN25_12040"
     CDS             4642..5976
                     /gene="rsmB"
                     /locus_tag="VLN25_12040"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF008149.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HSQ11323.1"
                     /translation="MGEASGVSPPLADVLATSARAWSDFVTGQSIDRAIAATVPADRA
                     RLRAAVQDTTYGAIRQRALSQRLIAELASRPPAAETAALLCVALPQLMAGRHAAHTVV
                     DQAVAAARMQPATVGAAGFVNAVLRNFLRRQDELLERLQRIEEVRYNAPAWWIDAVRI
                     AHPELWRGILATGFDAPALVLRVNRRRGTVEDYLQCLAEQELSATRVGLQAVWLQQAV
                     PVARIPGFAAGDVSVQDAGAQLAGAWLDVADGMRVLDACAAPGGKTAHLAELADLDLT
                     ALETDKQRAPRIEENLRRIERGANLIVADGRDVPAWWDGRPYDRILLDAPCTASGIVR
                     RHPDIPWLRRKADVAHLATLQAQLLEALWPLLGVGGRLLYVVCSVFPQEGSEQIARFV
                     GRHPDAAARPLPGGVPFVQLLPSGATGSIWDAGRPWPTIHDGFFYALLEKSR"
     gene            5973..6557
                     /locus_tag="VLN25_12045"
     CDS             5973..6557
                     /locus_tag="VLN25_12045"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HSQ11324.1"
                     /translation="MNLATVLRASIVTLLLAWLPAAPVLADERIGVIAAALEPAPAGA
                     GGVVLNATFEFEMPQVLEEAVTKGIAIYFNIEFELFRKRWYWLDRQISSSTLTYRLSY
                     SPLTRQYRLARGGLSQPFESLDEALGLLKSVRQWKVIEAGVLSPRDDYEAQVRMRLDV
                     TQLPKPFQVNAITSREWNLASDWRELAVPAELAR"
     gene            6647..8992
                     /locus_tag="VLN25_12050"
     CDS             6647..8992
                     /locus_tag="VLN25_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSQ11325.1"
                     /translation="MSRVLRYGLLFAIALSGVLLFILASASGNTAFFERNYPILLAVN
                     GVIAAALAVLVLLLVRRLAKRLLLKRFGARLTTRFAVAFALMGVLPGVLIYIVSTQFL
                     SRSIESWFDVRVDRALDSGLTLGRAALDNVKDDVAAKANAWALELADLPEAAQLSQLN
                     RLREQGGIDEALLVTGNGQLIGASGARVSELVPEAPKPSELRQARTQRMFAVIEGEAG
                     AVDAHQGLRARVIVPIPAPTTMSIRGSLLPPEGTGGRLHDSLGSRGGLPLAGSSMSPS
                     SDTRYLQLIQPVPPTIAANADALQNGYREYQELSLSRSGLKTIYGLTLTLTLLLSIFA
                     AVAGSVLLASSMTAPLLQLAEGTKAVAEGNFRPVREFPGNDEMNLLTQSFNAMTRQLT
                     DARDAVETKQRELENAKTYLERVLANLSAGVIVLDHEFRLVTANFGASRILGLSLGED
                     IGRPLQAIDGELADAFSAGFAEQALSGTPKDSWQQQIEVKRAVGTTVEPVMLLARGSR
                     LPLDDGPGYVIVFDDITQVISAQRAVAWGEVARRLAHEIKNPLTPIQLAAERLQMKLV
                     DKLAGGEADVLRRGTMTIVNQVAAMKRMVDDFRDYARVPPARLQPLDLNALIEEVVTL
                     YRTAGAPISLTLASPLPMIEGDATQLRQVIHNLIGNAQEALSGQPDPSIRIRTTLAEN
                     VEATGVVRAVRFSVEDNGPGFAANILRRAFEPYVTTKPSGTGLGLAMVKKIIDEHGAH
                     VEIVNRGGGSGVGHAGGAAVTIVFRRLVSAETADASPRRVA"
     gene            9124..9903
                     /locus_tag="VLN25_12055"
     CDS             9124..9903
                     /locus_tag="VLN25_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSQ11326.1"
                     /translation="MSTILVVDDEVGIRELLSEILSDEGHSVLTAENAARARDVRAQE
                     PIDLVLLDIWMPDTDGVTLLKEWASLGQLTMPVIMMSGHATIDTAVEATRYGALEFLE
                     KPIAMARLLSAVRSGLERGRVMRNLRPQGAASSYAPAVPAEIPVVAPTAPVAANEPVG
                     AHNGVPLDQPLRDARDQFERVYFEFHLAREHYSMTRVADRAGLERTHLYRKLKQLGIE
                     LARGARRDRAAEEPPAAAPTIASSALPSPPPVYVHRDEYDD"
     gene            9937..11349
                     /gene="trkA"
                     /locus_tag="VLN25_12060"
     CDS             9937..11349
                     /gene="trkA"
                     /locus_tag="VLN25_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HSQ11327.1"
                     /translation="MKIIIIGAGRVGSSVAESLVSEANDITIIDSDPASVADLQDRFD
                     LRGLVGNAALPSTLKQAGAEDCDMLIAVTASDEANLVACKISADLYNIPTRIARVRNV
                     ELQKHPKLIGDEGFRATHVIFPEQTVTDYLLKLIEFPEALQVLEFADGRASLIAVRAF
                     AGGPLVSHPVRELRRHLPNVDTRIVAIFREEASVRVDGDTMVQPGDEVFFLAASQNIR
                     TVMSELRRMDKPVRRVMLAGGGNIGLRVARSLDTGEGLDTPDRFIVKIIEANKRRAEL
                     LAHQLSTDVLVLSGDATDEDLLQQEGVHETDLFIALTNDDENNIMSSLLAKRMGARRV
                     IALINRKSYGDLMQGGQIDIAISPSYATLSALLKFVRRGDVVAAHSLRRGAAEALEII
                     AHGDARTSRIIGKSIDQVELPRGVTIGAVVRGAGERTQVLMAHHDTVIEPEDHLIVFV
                     ENKRLIQRVERLFTVGAGFF"
     gene            11555..12679
                     /locus_tag="VLN25_12065"
     CDS             11555..12679
                     /locus_tag="VLN25_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HSQ11328.1"
                     /translation="MDFIKSKDAAPTRRKFLSTAAAATAGTAALGFPMVSTAQTTTLR
                     FQSTWPTKDIFHEYATDFSKKVNEMAGNRLKIEVLPAGAVVPAFQLLEAVAKGTLDGG
                     HGVVAYHYGKNSALALWGSGPSFGMDPNMLLSWHYYGGGDALLQEIYKSINVDVVSYL
                     YGPMPTQPFGWFKKPIAKVEDVKGIKFRTVGLAVDMYTDMGAAVNPLPGGEIVPALDR
                     GLIDGAEFNNASSDKLLGFPDVSKNCMLQSFHQSGEQFEILFNKTKYAALAPDLKKII
                     DHAVEASSADMSWKAIDRNSKDYIELKKMGVNFYKTPDAILRAQLASWDKIMEKKSAE
                     NALFKKVLDSQKAFAQRASSWQNDYTVDFKMASNYYFGKK"
     gene            12758..>13039
                     /locus_tag="VLN25_12070"
     CDS             12758..>13039
                     /locus_tag="VLN25_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HSQ11329.1"
                     /translation="MQTLLLAVDRLSTWLGQLFAWSIVLLTLMISWEVYSRYAMNEPH
                     DWALNLQIMMYGTLFMMAGAYTLSKNGHVRGDVLYGFFRPRTQAGLDLAL"
BASE COUNT         2170 a         4269 c         4409 g         2191 t
ORIGIN      
        1 ctcggacggc gagatcaccc accagatccc ggtcgacgaa gtggtctaca tccaggccga
       61 cgacaagtac acctgcgtct actcgcgcga cggcaaggaa ctcgccgagt ggctgatccg
      121 gattcccttg tccgagctgt cagcgcagct ggatccgacc gattttgcgc agatccatcg
      181 ctcggtcatc gtcaacctgc attcggtgac cggtacgcgc cgcgatctca ccggcaagct
      241 ctacgtccgg ctgcgcgatc atccccgcga gttgccggtg tcacgtcagt acctgcacct
      301 gttccagaag atgtgatccg cgccgctgtc acgcggccgt gcggtcaccg atcccgtgcg
      361 ccgcgttggc gatgtgttcg acgatcgccg cggcaatttg cggctgcaac gcaggcggaa
      421 agtcgtgtcc catcccggcg atgaccttga gctgtgcgcc gcggatgtga tgcgcggtgt
      481 cgaggcctgc cgcgagcggg accaggggat cgtcgcggcc atggatcacc agcgtgggcg
      541 cggtgatctg cgcgagcagt cggcgccggt cgcccgacgc cagaatcgca agcagctggc
      601 gcgcggcgcc cgccgggtag tagccgcggc gcgcgacacg ctcgaggtgc tcgcgcagcg
      661 cgttcggttc gctcgggaaa cccggactgc cgatcacgct gaacacccgc atcaggtggt
      721 cggtgagctg cgcgacgtcg tcagtgctgc gcggccggtc gagaatcgcg cgcagcgcgc
      781 ggggcttgcc gagcgcgaca tgtggattcg ggttgccgct cgacgacatg atcgaggtca
      841 gggtccgcac gcgctgcgga tgccgcgccg cgaagacctg cgcgatcatg ccgcccatcg
      901 aggcacccac cacgtgggca cgcgtgatgc cgagcgcggc cagcagatcg gccgcgtcct
      961 gcgccatgtc gtcgagcgtg tacggtgctc gcaccggcag gcgcagcagc gcgcgcatga
     1021 tcgccagctg caggttcggc atgggcgcac tgcgcagctt ggtcgaatga ccacagtcgc
     1081 gattgtcgaa tcggatcacc cgcaatccgg agcgcaccag gggctcgatg aaactgtcgg
     1141 gccaggccgc cgccggcatg cccaggccca tgatcagcag caccacgggc gcgtcagcgt
     1201 cgccgaactc ctccacgtcg agcgtgacgc cgttggcgtc gatctggggc atggctgccg
     1261 gcgaccggag ttctagaacc acgccttgcg cgtggggtcg cgatacagcc agcgcagagg
     1321 gccgctccga tcgaatggct gccactggcc ctcgggctgg gcgagccggt cggcaattgc
     1381 gtaccaggcc agtgcgttca ggccgatgcc gaagtggctg gcctcgacct cgatgttctc
     1441 ggcgtgcgcg ctgcggcgct cgacgctgca ttgccaggcc accacgccgt cggtgcggct
     1501 gtagatcgat gtcgtgggaa ccggcggtgc ctggcgcagc ggctcgtgga tctcgggcgc
     1561 gccgatgcgg tgtccggtca ccaattcgta caggcgccat gcattcgttg ccttcggatg
     1621 cccggcgaac ggcgtgccaa gggtgatcac ctgccgcacg tcgcgcggga agtccttcgc
     1681 gagttcgcgc gcatagatcc cgcccaggct ccagccgatc aggctcacct tgcgcctgtg
     1741 ctggcgacgc agtttctgca cacgttgcat gcagtggtcc agcacgccgt ccttcgggcc
     1801 gaggttcgag ccgtgctccc atccgtaggc gaggtagccc ttgcgctcga ggaactttcg
     1861 cagcggcttg gtcgacgcgt ccgacgccat caggcccgga aagacgagca ccgggtggcc
     1921 atcgccgtcc gccgcctggt cgagccacgg gctggccagc atcgtcgcgc cgtattccca
     1981 cggcgcgcgc cactccagtg cgagccggac gaatcccggc gcacgcatca tgtcaaagtc
     2041 gtaccagtgc tgcgagttat ccatgagtcg atgacatcgt gcggaaagcg tgatggttgg
     2101 ccatgggatt gcggctacct tgcggccgct cgacgccgcg gcggcgcctt ggcggtgtcg
     2161 ggctcgacgc gcggggacgc cgcgatgtcg gtggcgcgcc ggagttcggc cagggcttcg
     2221 tgcagcccgc gcgccagggg ccgggcgtcc gggatgccga cccgcgccgc gagaatgccg
     2281 aagtcgagcg agccggcgta gctttgcagc gtgatgttca ggccgaggcc gtgctcgacg
     2341 atcgacaccg gccagtaggt cttcaggcgg gctcccgcga gatacagcgg cgcgtcggga
     2401 ccgggcacgt tggacaccac gaggttggcg atgggtggga tgcgatcggc gagcgaggtc
     2461 cggccataca gggcggcggc ggctgacagc agccacggca ggccgagcga cggaaagtcc
     2521 gtcggtatta cgcccttgat ttgccgtgtc acgtctttcg ctgcgcgtgt cgatgcctga
     2581 accgcgcgca gccgctcgat cggatcggcc agcgttgtag ccagtgacgc gagcgtcatc
     2641 gtcgccagcg tggtcgcttc ggtgttgccc gcttcgcgca gtgacaccgg catcgcggcc
     2701 accagcgact ttgtgggcac gccgccgcgc gccttgaggt acccgcgcaa cgcgccgctc
     2761 accagggtca gcacgacgtc gttcagcgtc gcttcgtgcg cggcagcgat cgcgcgcaca
     2821 tcggccagcg gcagcgacgc agtcgcaaag ctgcgcccgg agtcgatcac acgattgaac
     2881 gcggtgcgcg ggccgaaggc gaggttgcga ctgaaaccgg tcgagccggt gacgagccgc
     2941 gcggccacac gcgccagcgc gggaaggcgg cgtaccaggt cgactgtctg tgacccgctg
     3001 accttgagcg cggtgccgac catcatcacg aggcccggtt gatcgcggcg gcgcttgcgc
     3061 gccggctcga ccgcgcgcgg cgacggcgcc aggtcgagca gtgcgtgggc cagcgccacg
     3121 cccgaggcgc cgtcgagcgt cgcatggtgg atcttcgtgt agaaccccag cccgccatcc
     3181 tgcaggcctt cgatcacgtg aaaacgccag agcggtcggg agcgatcgag tggctgtgca
     3241 tgcaggcggg cgacggccgc ctcgagctgc gcgagcgttc ccggcgccgg cagcgtgacg
     3301 cgcacaacgt gccgctccag gtcgacctgc tcggcgcgga cccataccgg attggcgaaa
     3361 tccagcggca tatgcgctaa ctgccgcgtg aacactggcg acaggtgcac gcgccgcgcc
     3421 acatgccgtc gcacgcgtag caggtagtcg cccccgcgcc ggggtcgctc cagcaggtga
     3481 acggcgccga catgcatagg cgtggccggt gtttccagat gcaggaacag tgaatccagc
     3541 cccgacagtg gcttcaagag tctcccttaa tgcgtgcggc tgcgagctct gatgacgctt
     3601 gcagaggccg atggtaatcg caatggccgg tgatggagcc cacctagaat gtcgcgatgc
     3661 gcatcgtatt cgcaggaact cccgacttcg ccgcgcgctc gctggcggca ctgatcgcgg
     3721 cagcgccggc cgcgggctgg tcgctgcccc tggtgctgac gcagccggat cggcccgccg
     3781 ggcgcggcct gcgcctgacg ccgagtccgg tgaagtccct cgcgcttgcg catggcctta
     3841 cggtcgacac gccggtgtca ttgcgcaagg ggccggaggc cgaagcagcg cagaggtgga
     3901 ttgccgcggt cgagcccgac gtgctgatcg tcgcggccta tggcctgatc ctgccgcagt
     3961 cagtgctcga tataccgcgt ggcctcaggc ccgggtggtc gccgaagatc accgccatca
     4021 acgtccatgc gtcgctgctg ccgcgctggc gcggcgccgc gcccattgca cgcgccatag
     4081 aggcaggcga ctccgtgacc ggtatcacca tcatgcagat ggacgccgga ctcgataccg
     4141 gccccatgct gcttgccgag tcgatcgaca tcgacgccga cgagaccgct ggacaactca
     4201 ctgcccggtt ggcggacctg ggtgcgactc tccttgtccg ggcactcgtc tccattgccc
     4261 agctcagcgc gcagccccaa tcggccgcgg gcgccaccta tgccgccaag atcggcaaag
     4321 cggaagcatg gctcgactgg tcgttgccgg cagcggtcct ggcacgcaag gtgcgggcct
     4381 acgacccgtt tccggtggca cacgcgcgca tcggcgaagc ttcgatcaag gtctggcgcg
     4441 cgatagcggt accggcaacg gcggaggttg caccgggcac gattgtcgct gcgggcgccg
     4501 ccggaatcga cgtgcgctgc ggccacggat gcctgcggct gacggagctg caacgtccgg
     4561 gaggcaaacg gctcgcggca cgcgagttcc tggcgggcca tgcgctggcg ccgctgcagt
     4621 gcttcgacgt cggaccggcg catgggtgag gccagcggcg tctcgccgcc gctcgctgac
     4681 gtgctggcca cgtcggcgcg tgcgtggagc gatttcgtga cggggcagtc aatcgatcgt
     4741 gccatcgcgg cgaccgttcc cgccgatcgc gcgcgcttgc gcgccgccgt gcaggacacg
     4801 acctatggcg ccattcggca gcgcgcactc agccagcggc tgattgccga gctggcgtcg
     4861 cggcctccgg cggcggagac ggcggcgctg ctatgcgtcg cgttgccaca gttgatggca
     4921 ggccgtcatg cagcacacac cgtcgttgat caggccgtgg cggcggcgcg catgcagccg
     4981 gcgacggtcg gtgccgctgg cttcgtcaac gcggtactgc gcaactttct gcgtcggcag
     5041 gacgaattgc tcgaacgcct gcagcgcatc gaagaggtgc gctacaacgc gcccgcgtgg
     5101 tggatcgacg ccgtgcgtat cgcgcacccg gagctatggc gcgggatcct tgcgaccgga
     5161 ttcgacgcgc ccgcactggt gctgcgcgtg aaccggcggc gcggcaccgt tgaggactat
     5221 ctgcagtgcc tcgctgagca ggagttgtct gccactcgag tcgggctcca ggccgtgtgg
     5281 ctgcaacagg cggtaccggt tgcgcgcatt ccaggttttg cggcaggcga tgtgtcggtg
     5341 caggatgcag gggcgcagct ggccggcgcg tggctcgacg tggccgacgg catgcgagtc
     5401 ctcgatgcct gcgctgcccc cgggggcaag accgcgcacc tggctgagct ggccgatctc
     5461 gacctgaccg cgctcgagac ggacaagcaa cgtgccccgc gcatcgaaga gaacctgcgc
     5521 cgcatcgaac gtggagcgaa cctcatcgtc gccgatgggc gagacgtacc ggcatggtgg
     5581 gacggccggc cttacgaccg catcctgctc gatgcgccgt gcaccgccag cggcatcgtt
     5641 cgccggcatc cggacatccc ctggctacgc cgtaaggccg atgttgcgca tttggcaacg
     5701 ctgcaggcgc agttgttgga ggctttgtgg ccgctgctgg gggtgggtgg tagattgctt
     5761 tacgtcgtgt gctcggtgtt tccacaggaa gggagcgagc agatcgcgcg ctttgtcggg
     5821 cggcatccgg acgctgcggc gcggccgctt cccggcggtg tgccgttcgt gcagttgctg
     5881 ccatccgggg caacaggctc gatctgggac gccggccggc catggccgac gattcacgac
     5941 ggttttttct acgcacttct cgagaaatcc cgatgaacct ggccaccgtg ctgcgcgcgt
     6001 cgatcgtcac gctgctgctg gcgtggcttc ccgccgcgcc cgtgctggcg gacgagcgca
     6061 tcggcgtcat cgcagccgcg ctggagccgg caccggccgg tgccggcggt gtggtgctca
     6121 atgccacctt cgaattcgag atgccgcagg tgctcgaaga agcggtgacc aaaggcatcg
     6181 cgatctactt caacatcgag ttcgagctgt ttcgcaagcg ctggtactgg ctggatcggc
     6241 agatcagttc cagcacgctc acgtatcgcc tgtcctacag tccgttgacg cggcagtacc
     6301 ggctcgcccg cggtggcctg tcgcagccgt tcgaatcgct cgacgaggcg ttgggacttc
     6361 tgaagagcgt gcgccagtgg aaggtgatcg aagccggtgt gctgtcgccg cgcgatgact
     6421 acgaggccca ggtgcggatg cgcctcgacg tgacgcagct gcccaagccg ttccaggtca
     6481 acgccatcac gagccgcgag tggaatctcg cctcggactg gcgcgagctg gcggtgcccg
     6541 ccgaactggc gcgctgaggc catggatcag ccggagtcga gggtgcgcgg caagtcgggc
     6601 gccggtttgc tgacacttcg gctgaccttc cgagggcagg gttgagatgt cgcgcgtact
     6661 gcgatacggc ctgctgttcg cgattgcgct gtcaggcgtg ctgctgttca tcctggcctc
     6721 ggccagcggc aacaccgcgt tcttcgaacg caactaccca atcctcctcg ctgtcaacgg
     6781 cgtcatcgcg gcggcgctcg cggtgctggt cctgctgctg gtgcggcgcc tggccaagcg
     6841 cctgctgctg aagcgcttcg gagcgcgcct gaccacgcgc tttgctgttg cgttcgcgct
     6901 gatgggtgtt ttgcccggag tgctgatcta catcgtttcg acccagttcc tgtcacgctc
     6961 tatcgagtcc tggttcgacg tacgggtcga ccgcgcgctc gattcgggat tgacgctggg
     7021 acgtgctgct ctcgacaacg tgaaggacga cgtggccgcc aaggcgaatg cgtgggcgct
     7081 cgagctggcc gacctgcccg aggccgctca gctgtcgcag ctgaaccgcc tgcgcgagca
     7141 gggcgggatc gacgaggcac tgctggtcac cggcaacggg cagttgatcg gggcgtccgg
     7201 tgcccgggtc tcggaactgg tgcccgaggc gcccaagccc agcgagttgc gacaggcgcg
     7261 gacgcagcgc atgtttgccg tcatcgaagg ggaggccggc gcagtcgacg cgcaccaggg
     7321 tctgcgcgcc cgcgtcatcg tgccgattcc ggcgccgacg acgatgtcga tccggggttc
     7381 gctcctgccg cccgaaggga cgggcggacg gctgcacgac tcattgggca gtcggggtgg
     7441 cctgccgctg gccggcagca gcatgtcgcc gtcctcggac acacgctatc tgcaactgat
     7501 ccagccggtg ccaccgacca ttgcagcgaa cgccgacgcg ctgcagaacg gttaccggga
     7561 ataccaggag ctgtcgctgt cgcgcagcgg actgaagacg atctacgggc tgacgctgac
     7621 actgacgctg ctgctctcga ttttcgctgc agtcgccggc agcgtgctgc tcgccagttc
     7681 gatgacggcg ccgctgctcc agctcgccga aggcacgaaa gccgtcgccg agggcaactt
     7741 cagaccggtg cgcgagtttc cgggcaacga cgagatgaac ctgctgacgc agtcgttcaa
     7801 tgcgatgacg cggcagctga ccgatgcgcg ggatgccgtc gagaccaagc agcgggaact
     7861 cgagaacgcc aagacctacc tcgagcgcgt gctggccaac ctgtcggccg gggtgatcgt
     7921 gctcgaccac gagttccgcc tggtgacagc caacttcggc gcttcgcgca ttcttggcct
     7981 gtctctcggc gaggatatcg ggcgaccgct gcaggcgatc gacggagaac tcgccgacgc
     8041 attttcggcg ggcttcgccg agcaggcctt gtcgggcact cccaaggact catggcagca
     8101 gcagatcgag gtcaagcgcg cggtaggcac gacggtcgaa ccggtgatgt tgctggcacg
     8161 aggatcgcgc cttccgctcg acgacgggcc cggctatgtg atcgtgtttg acgacatcac
     8221 ccaggtgatt tctgcacagc gcgccgtggc atggggcgag gttgcgcgcc ggctggcgca
     8281 cgagatcaag aatccgctga caccgataca gctggccgcc gagcgcctgc agatgaagtt
     8341 ggtcgacaag ctggcgggcg gcgaggctga cgtcctgcga cgtggcacca tgactatcgt
     8401 caaccaggtg gcggcgatga agcgcatggt cgacgacttc cgcgattacg cgcgcgttcc
     8461 gcccgcccgc ctgcagccgc tcgacctgaa cgcgctgatc gaggaagtcg ttaccttgta
     8521 ccgtactgcc ggtgcgccga tttcgctgac gctggcgtcg ccgctgccca tgatcgaagg
     8581 cgatgcgacg cagctgcggc aggtgatcca caacctgatc ggcaatgcgc aggaagcgct
     8641 gagcggccag cctgacccga gcatacggat ccggaccacg ctggccgaaa acgtggaagc
     8701 gaccggagtt gtgcgcgccg tccgtttctc cgtcgaggac aacggcccgg gcttcgcggc
     8761 gaatatactg cgacgtgcgt tcgagcctta cgtcaccacc aaaccgagcg gcacgggtct
     8821 tggactggcc atggtcaaga aaatcatcga tgaacacgga gcgcatgtcg aaatcgtcaa
     8881 ccgtggcggc ggaagcggcg ttggccacgc gggcggtgcc gcggtgacga tcgtgtttcg
     8941 tcggctggta tcggccgaga ccgccgacgc cagcccgcgt cgtgtggcct gagccttatg
     9001 agcctaagac ccgaagaccc gaagactcga taacctgccc ggaatccatg atcccgttgc
     9061 ccacgcgaat ccttcgatgc ccgcgccacc cggcgtggct cccgttcaca gaccggtaac
     9121 ttcatgtcga ccatcctagt cgtcgatgac gaagtcggca tccgcgaact cctgtcggag
     9181 atcctgagcg acgaaggtca ttcggtactg actgccgaga acgccgcacg ggcgcgggat
     9241 gtgcgcgcgc aggagcccat cgatctggtg ctgctcgata tctggatgcc cgacactgac
     9301 ggcgtcacgc tgctcaagga atgggcgtcg ctgggccagc tgacgatgcc ggtcatcatg
     9361 atgtcgggcc acgcgaccat cgataccgcg gtcgaagcga cgcgttacgg tgcgctcgag
     9421 ttcctcgaaa agccgatcgc catggcgcgc ctgctgtctg cggtgcgttc cggtctcgaa
     9481 cgcggacgcg tgatgcgcaa cctgcgccca cagggtgccg cgtcgtccta tgcacctgcc
     9541 gtcccggccg agataccggt cgtcgcaccg accgcgccgg tggccgcgaa tgagccggtc
     9601 ggcgcacaca acggcgtgcc gctggaccag ccgctgcgcg atgctcgcga ccagttcgag
     9661 cgcgtctact ttgaattcca tcttgcgcgc gagcactaca gcatgacgcg ggtcgcggat
     9721 cgtgccggtc tcgaacgcac gcatctgtat cgcaagctga agcagctcgg catcgagctc
     9781 gcccgcggtg cccgccgtga tcgcgccgcc gaggagcctc cggccgccgc gccgacgatt
     9841 gcgtcgagtg cgctgccatc gcccccgccg gtctacgtgc atcgggacga atacgacgac
     9901 tagccctgcc gccgggccgg ctgcgggccc gcattcatga agatcatcat cattggtgcc
     9961 ggtcgggtcg ggtcctcggt ggccgagagc ctggtgtccg aagccaacga catcacgatc
    10021 atcgattccg atccggcgag cgtggcggat ctgcaggatc gcttcgacct gcgtggactg
    10081 gtcggcaacg ccgcactgcc gtcgacgctg aagcaggcag gcgccgagga ctgcgacatg
    10141 ctgatcgctg tcacggcctc cgacgaggcc aatctcgtcg cctgcaagat ctccgcggac
    10201 ctgtacaaca ttcctacacg catcgcgcgc gtgcgcaacg tggagttgca gaagcacccg
    10261 aagctcatcg gcgacgaagg cttccgcgca acgcacgtga tctttccgga gcagaccgtc
    10321 accgactacc tgctcaagct gatcgagttt ccggaggcac tgcaggtact cgagttcgcc
    10381 gacggccgcg cgagcctgat cgcagtgcgg gcgtttgccg gcgggccgtt ggtgtcgcat
    10441 ccggtcaggg agctgcgccg ccatctgccc aatgtggata cgcgcatcgt cgcgatcttc
    10501 cgcgaggaag ccagcgtgcg ggtcgacggc gacaccatgg tgcagccggg cgacgaggtc
    10561 tttttcctgg ccgcctcgca gaacatccgc accgtgatga gcgagctgcg tcgcatggac
    10621 aagccggtgc ggcgggtgat gctcgccggc ggcggcaaca tcggcctgcg ggtggcacgc
    10681 tcgctcgaca cgggcgaggg gctggacacc ccggatcgct tcatcgtcaa gatcatcgag
    10741 gcgaacaagc ggcgcgccga actcctcgcg caccagctgt cgaccgatgt gctcgtgctg
    10801 agcggcgacg ccacggatga agacctgctg caacaggagg gcgtgcatga aacggatctg
    10861 ttcatcgcgc tgacgaacga cgacgaaaac aacatcatga gttcgttgct ggccaagcgc
    10921 atgggcgcgc gccgcgtcat cgcactcatc aaccgcaaga gctacggcga cctgatgcag
    10981 ggcggacaga tcgacatcgc gatctcgccg tcctacgcca cgctgtcggc cctgctgaag
    11041 ttcgtgcgcc ggggcgacgt ggtcgccgcg cacagcctca ggcgcggggc tgccgaagcg
    11101 ctcgagatca tcgcccacgg tgacgcgcgc acctcgcgca tcatcggcaa gagcatcgat
    11161 caggtggagc ttccccgcgg tgtcaccatc ggcgcagtgg tacggggcgc gggcgagcgg
    11221 acccaggtgc tgatggcaca ccacgacacg gtgatcgagc cggaggacca tctgatcgtc
    11281 ttcgtcgaga acaagcgtct gatccagcga gtcgaacggt tgttcaccgt gggagccggc
    11341 ttcttctgat cggatgctgc gacgcaacga tccgatgtgc ggtgcgcgtt ttcccccggc
    11401 ggccccggaa tcatttcccc cccgggttcc gattcaatag aatcgctgac ggcggcacta
    11461 ccctgcgacc caagcgaaac aagccgcacc caacagaatc ggcctcgggc ccgacacgtc
    11521 gcagtttcaa acggcttcag ccaaggagac attcatggat ttcatcaaga gcaaggacgc
    11581 cgcaccgacg cgtcggaaat ttttgtccac ggccgctgcg gccaccgcgg gcaccgcggc
    11641 gctgggtttc ccgatggtgt cgacggccca gaccacgacg ctgcggttcc agagcacctg
    11701 gccgaccaag gacatcttcc atgagtacgc gaccgacttc tccaagaagg tcaacgaaat
    11761 ggccggcaac cggctgaaga tcgaagtgct tccggcgggc gctgtcgttc cggcgttcca
    11821 gcttctcgaa gccgttgcga agggcacgct cgacggcggt catggcgtcg ttgcctacca
    11881 ctacggcaag aactccgcgc tcgcgctgtg gggttcgggt ccgtcgttcg gcatggatcc
    11941 gaacatgctg ctgtcctggc actactacgg cggcggtgat gcgcttctgc aggagatcta
    12001 caagtcgatc aacgtcgatg tggtctcgta cctttacggg ccgatgccaa cgcagccctt
    12061 cggctggttt aaaaagccga tcgccaaggt cgaagacgtc aagggcatca aattccgcac
    12121 ggtgggcctg gcggtggaca tgtacaccga tatgggcgca gcagtgaacc cgctgcccgg
    12181 tggcgaaatc gtgccggcgc tcgaccgcgg cctgatcgat ggcgccgagt tcaacaacgc
    12241 atcgtcagac aagctgctcg gctttcccga cgtcagcaag aactgcatgc tgcagagctt
    12301 ccaccagagc ggtgagcagt tcgaaatcct gttcaacaag accaagtacg ctgccctggc
    12361 gccggacctc aagaagatca tcgaccacgc cgttgaggcc tccagtgccg acatgagctg
    12421 gaaggcgatc gaccgcaact cgaaggacta catcgagttg aagaaaatgg gcgtgaactt
    12481 ctacaagacc cccgacgcga tcctgcgcgc gcagctcgcc tcgtgggaca agatcatgga
    12541 gaagaagtcc gcggaaaatg cgctgttcaa gaaggtgctc gactcgcaga aggccttcgc
    12601 gcagcgtgcc agctcgtggc agaacgacta cacggtcgac ttcaagatgg cctccaacta
    12661 ctacttcggc aagaaatagc cacctagttg aggtccgatg ggccacctgc cagcagcaag
    12721 ggtcggtggc cctttttctt cccgtaaagg aagggcgatg cagacactcc tgctggcggt
    12781 ggaccgcctg tcgacatggc tgggccagtt gttcgcctgg tccatcgttc tgttgacgct
    12841 gatgatcagc tgggaggtct actcccgcta cgcgatgaac gaaccgcacg actgggctct
    12901 gaacctgcag atcatgatgt acgggacgct cttcatgatg gccggcgcgt acacgctgtc
    12961 gaagaacggc cacgtgcgcg gcgacgtgct ctacggcttc ttccgcccgc ggacgcaggc
    13021 cggcctcgac ctcgcgctg
//
LOCUS       DATIRT010000525         7839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_193686, whole genome shotgun sequence.
ACCESSION   DATIRT010000525 DATIRT010000000
VERSION     DATIRT010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7839
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193686"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..1804
                     /gene="dnaG"
                     /locus_tag="VLN25_12075"
     CDS             20..1804
                     /gene="dnaG"
                     /locus_tag="VLN25_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HSQ11330.1"
                     /translation="MIPQSFIQDLLARVDIVDVVGRHVQLKKGGQNFLGLCPFHGEKS
                     PSFTVSPSKQFYHCFGCGVHGSAIGFLMELRGLGYVEAIRELAQQAGLQVPESTERNR
                     DAASKVSALSDLLKRAADYYRVQLKDSPVAVDYLKGRGLTGQTAARFALGYAPDAWQP
                     LRAAVDNYDDPRLVEAGLVIADEGKRYDRFRGRIMFPIRNARGQVIGFGARVLGQGEP
                     KYLNSPETPVFRKGQELYGLHEARESIRQSGRAIVCEGYLDVIQLAQAGFGEAVAALG
                     TAVTSAHVSALMRNTDHVIFAFDGDAAGRKAARRALEATLPVIADTKRASFVLLPEGE
                     DPDSIVQRGGAPAFAEELAQALPLSRFFIESLASGRSLASPEDRAAIVADARPLVLSM
                     PPGALRLQLLHELAEAARTPLDDIETLFGLRRERRNRPAPMARRTPHAEVEDLKRRIL
                     QQLLTHPALAREFSPAVSEEHVGRDEPIDREIAEVWTMATAPGPSAGAVMTHGALLEL
                     MQDSPFIDDYRALAAQEMEMDTDVETARQVLEEAFHKLQLRRLEQERSERLAEFEREQ
                     SPERLNAYRAADLAYTQARGRSIEARPQ"
     gene            2212..4512
                     /gene="rpoD"
                     /locus_tag="VLN25_12080"
     CDS             2212..4512
                     /gene="rpoD"
                     /locus_tag="VLN25_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HSQ11331.1"
                     /translation="MVTKKPLVKPKSARSTAREVARPKSEVKKHPPVPLKKLVVAKPV
                     RAEAHLKALTKPVASKPTVPAKPATSKPVVSAESAAGVAEAVRGRPGRKPKSSEDQQA
                     GAEGGIDAVPEAGDDTADPSVQIVEKMSATKLRAKDRRAKEKALKEALERSYHGTEED
                     LEARRNKLKALIKLGKERGFLTYAEINDHLPDNLVDAEAIDSIISTFGDMGIQVFDQA
                     PDAETLLMNENVPAATTDDDVEEEAEAALTTVDAEFGRTTDPVRMYMREMGSVELLTR
                     EGEIEIAKRIEDGLKHMVMAVSACPTTIAEILAHSERIRASEIPIDEVVDGLMDPTAE
                     DFPTAGGDEEDEEGEETTDIGASGMTAAQLDELKVRAIEKFDHVAKLFDKMRVAFEKE
                     GYKSPGYLKSQTKILDELMSLRFTAKMVEKLSDTLRKQVDEVRSLEKQIYEIVVNKCG
                     MPRAHFLKAFPGNETNMRWAAREADSGQPYAEVLKRNLPAVQELQSKLSDLQKRVVLP
                     LRDLREVYKQMATGEAKARKAKREMTEANLRLVISIAKKYTNRGLQFLDLIQEGNIGL
                     MKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQI
                     LQETGTEPDPATLAVKMEMPEDKIRKILKIAKEPISMETPIGDDDDSHLGDFIEDQTT
                     VAPVDAAQYNSLSGVTKDVLDSLTPREAKVLRMRFGIEMSTDHTLEEVGKQFDVTRER
                     IRQIEAKALRKLRHPSRSDKLKSFLE"
     gene            4584..4660
                     /locus_tag="VLN25_12085"
     tRNA            4584..4660
                     /locus_tag="VLN25_12085"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4618..4620,aa:Ile,seq:cat)
     gene            4755..6116
                     /locus_tag="VLN25_12090"
     CDS             4755..6116
                     /locus_tag="VLN25_12090"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006497050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSQ11332.1"
                     /translation="MGYTTPTPIQSKAIPLVMQGRDVMGAAQTGTGKTAGFALPIIQR
                     LLPQSSTSMSPARHPVRALVLTPTRELADQVAENVKLYITGTPLRCAVVYGGVDMDPQ
                     TQALRTGVEIIVATPGRLLDHLQSKNTSLGQVQIVVLDEADRMLDMGFLPDISRILNH
                     LPKERQSLMFSATFSEEIKKLAANFLRDPVLVEVARRNATAENVTQEVVKLHESEKTD
                     VLLELLRTRGEGGGRLTQVLVFVNSKIECRRLARQLQKADVNADAIHGDKTQDERMKA
                     LEGFKTGTIEVLVATDVAARGLDIVELPVVINYDVPFSPEDYVHRIGRTGRAGATGIA
                     IMMMTGSDERNVAAIERLTKQKFQAVSVSPERGERSPQRSASRGGRERPERDDSRRGR
                     SEPAVEPRRAPTRPADDFFSRPYEPGAAGAEPAVPPPTESALPQPARPGGRVAALLGG
                     RKK"
     gene            complement(6178..7293)
                     /locus_tag="VLN25_12095"
     CDS             complement(6178..7293)
                     /locus_tag="VLN25_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSQ11333.1"
                     /translation="MYQAPPPPALLVIDDLAVTYRGGSRVLEQLTLSLERGEIGCLVG
                     SSGCGKTTLLRAIAGFLPVDAGTIEIDGLVVSGPHFTAPPEKRGVGLVFQDYALFPHL
                     KVVDNIAFGLRALRGSARTARVDEMVALVGLASEAQRYPHELSGGQQQRVALARALAP
                     KPALLLMDEPFSSLDVELRQRLGHEVRQILKASGTTAILVTHDQQEAFAIADRVGVMH
                     DGNLEQWDRPYELYHRPATRYVADFIGQGVFLPAQVLSARNVEIELGELRGEIPLPCQ
                     QGCATCGKGCHVEVLLRPDDVIHDDASPLKAEVKRKVFRGGDFIYTLRLESGREVLAL
                     VPSHHDHAIGEMIGIRLDADHVITFPARSPQDQCNVG"
     gene            7365..>7839
                     /locus_tag="VLN25_12100"
     CDS             7365..>7839
                     /locus_tag="VLN25_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HSQ11334.1"
                     /translation="MRTEIQTNPADARAPVARRRSAPIESALGRPLLLALVCTALVGL
                     PVLSIFASFVDTAASAETLRHLAATVIPDAMVETFVLGGGVVIGVVILGSATGWIAAS
                     CEFPGRRITEWALLLPLAMPAYIVAYAYTDALQFSGPMQSALREGFGWRRGDYWFP"
BASE COUNT         1554 a         2489 c         2523 g         1273 t
ORIGIN      
        1 tgcgtaaact ttcgaagcca tgatcccgca aagcttcatc caggacctgc tcgcgcgggt
       61 cgacatcgtc gatgtcgtcg gtcgccacgt gcagttgaaa aaaggcgggc agaatttcct
      121 ggggctgtgc ccgttccacg gggaaaagtc gccgtcgttc acagtcagtc catcgaagca
      181 gttctatcac tgctttggct gcggggtaca tggcagtgcc atcgggttcc tgatggagtt
      241 gcgcggtctc ggctatgtcg aggcgatccg cgaactggcg cagcaggcgg gtctgcaggt
      301 gccggagtcg accgagcgca accgcgatgc cgccagcaag gtcagtgcgc tgtccgactt
      361 gctcaagcgg gccgctgact actaccgcgt ccagctcaag gacagcccgg ttgcggtcga
      421 ctacctcaag ggccgaggcc tgacgggaca aaccgcagcc cggtttgccc tcggctacgc
      481 gccggatgcc tggcaaccgc tgcgcgccgc agttgacaac tacgacgacc cccgactggt
      541 cgaagccgga ctggtgatcg ccgacgaggg caagcgctac gaccggttcc gcggccggat
      601 catgttcccg atacgcaatg cccggggcca ggtgatcggt ttcggcgcgc gggtgctcgg
      661 acagggcgag ccgaagtatc tcaactctcc tgaaacaccc gtgtttcgca agggacagga
      721 gttgtatgga ctgcacgaag cgcgcgagtc gatccggcag agcggccgcg cgatcgtgtg
      781 cgaaggctac cttgacgtga tccagctcgc gcaggccgga ttcggcgaag cggttgcagc
      841 gctcggcact gcggtgacca gtgcgcatgt cagcgccttg atgcgaaaca cggaccatgt
      901 gatcttcgcg ttcgacggcg atgcggccgg gcgcaaggcg gcacgacgtg cgctggaggc
      961 gacgctgcca gtgattgccg ataccaagcg cgcgagtttt gtccttttgc ccgagggcga
     1021 agaccccgac agcatcgtgc agcgcggcgg cgcgcctgcg tttgccgagg agctggcgca
     1081 ggcactgccg ctgtctcgct tcttcatcga gtctctggcg tcgggccggt cgctggcgtc
     1141 gccggaggat cgggccgcca tcgtggccga cgcccggccg cttgtgcttt cgatgcctcc
     1201 cggcgcactt cggctgcaac tgctgcacga actggcggaa gcagcccgca cgccgctgga
     1261 tgacattgaa accctgttcg ggttgcgcag ggagcgccgc aaccgtcccg ccccgatggc
     1321 tcgccggacg ccccatgccg aggtcgaaga cctcaagcgc cgcatcctgc agcaactgct
     1381 gacgcaccca gcgctcgcaa gagaattcag cccggcggtc tccgaggagc acgttggccg
     1441 tgacgagccg atcgaccgcg aaattgcgga ggtctggacc atggcgacgg cacccggtcc
     1501 gtcggcgggt gccgtgatga cgcacggagc gctgctcgag ctcatgcagg acagtccgtt
     1561 cattgacgat taccgcgcgt tggccgccca ggaaatggaa atggacacgg acgtcgagac
     1621 ggcccgccag gtgctggagg aagccttcca caaactccaa ttgcgccgcc tggagcagga
     1681 gcgcagcgag cggctcgccg aattcgagcg ggaacagtcc cccgagcgac tgaatgcgta
     1741 tcgtgcggca gacctggcct atacgcaggc gcgtgggcgc agtatcgagg cgcgtcccca
     1801 gtaggcatcg gaggtatttg cggcgcccgg ctgcggcgtg gacgaagttt gctaatatgt
     1861 tgaattctcg acgtttcaag cggtttcggg cttcaggcag aaacgcagca ttacgcgtcg
     1921 gggcgcccag gagatcacga attgatcggt ggcgcagcaa ttcaggatcg accagcgtgc
     1981 aggatgcact gcagggcgcc cgtacgagga gcccggccct cgcggcaagt cagctcacgg
     2041 tcacgcggag atgcggcatc gagaccacgc tgtgatggcg gcggtcccga ttgagatcgc
     2101 gtgattgaac ccaatgatgt atgcaagccg tgccggcccg cgtcgttgca ggacgtacgc
     2161 gcttcccagt gacagttctg cccggcgtgg caggcgatcg gagtgagaac gatggtgacg
     2221 aaaaagccgc tggtgaagcc caaatccgca cggtcgacgg cgcgcgaagt ggcgcggccc
     2281 aagtccgagg tcaagaagca cccaccggtt ccattgaaga agctcgttgt cgccaaaccc
     2341 gtccgtgccg aggcgcatct caaggcactg acaaagccag tggcatcgaa gccgacggta
     2401 cccgccaagc ctgccacctc gaagcccgtc gtctcggccg agtcggctgc gggcgttgct
     2461 gaagcggtgc gcggccgtcc cggccgcaaa ccgaaatcgt ctgaggacca acaggcggga
     2521 gcagagggcg gtatcgatgc cgttccggaa gccggcgacg acacggccga ccccagtgtg
     2581 cagatcgtcg agaaaatgag cgcgaccaag ctgcgcgcca aggatcgacg agccaaggag
     2641 aaagccctca aggaagcgct cgagcgcagc tatcacggca ccgaggaaga cctcgaggca
     2701 cgccgcaaca agctgaaggc gctgatcaag ctgggcaagg aacgcggatt cctcacctac
     2761 gctgagatca acgaccatct gcccgacaac ctcgtcgacg ccgaggcgat cgactcgatc
     2821 atcagcacgt ttggcgacat gggcatccag gtcttcgacc aggcgcccga tgccgagacg
     2881 ctgctgatga acgagaacgt tccggccgcg accactgacg atgacgtcga ggaagaggcc
     2941 gaagctgcgc tgaccaccgt cgacgccgag ttcggccgca ccactgatcc ggtgcgcatg
     3001 tacatgcgcg agatgggatc tgtcgagctg ctgacgcgcg aaggtgagat cgagatcgcc
     3061 aagcgcatcg aggacggact caagcacatg gtgatggccg tgtcggcttg cccgacgacc
     3121 atcgccgaga tcctcgctca ctcggagcgt atccgcgcga gcgagatccc gatcgacgaa
     3181 gtggtcgatg gactgatgga tccgacggcc gaagacttcc cgacggccgg cggcgacgaa
     3241 gaggacgagg aaggcgagga aaccaccgac atcggtgcat ccggcatgac ggcggcccag
     3301 ctcgatgagc tgaaagtccg cgcgatcgaa aagttcgatc acgtcgccaa gctcttcgac
     3361 aagatgcgag tggcattcga gaaggaaggc tacaagtcgc cggggtacct gaagtcccag
     3421 accaagatcc tcgacgagct gatgagcctg cgcttcacgg ccaagatggt cgagaagctg
     3481 tcggacacgc tgcgcaagca ggtcgacgag gtgcgcagcc tcgaaaagca gatctacgag
     3541 atcgtcgtca acaagtgcgg catgccgcgc gctcatttcc tcaaggcgtt tccgggcaac
     3601 gagaccaaca tgcgctgggc cgcgcgcgaa gccgattcgg ggcagcccta cgccgaagtg
     3661 ctgaagcgca acctgcccgc agtgcaggaa ctgcagagca aactctcgga cctgcagaag
     3721 cgtgtcgtgc tgccgctgcg cgacctgcgc gaggtctaca agcagatggc caccggcgag
     3781 gccaaggcgc gcaaggccaa gcgcgagatg acagaggcca acctgcgcct ggtgatctcg
     3841 attgcgaaga aatacaccaa ccgcggcctg cagttcctcg acctgattca ggaaggcaac
     3901 atcggcctga tgaaggcggt cgacaagttc gagtaccgcc gcggttacaa gttctcgacc
     3961 tacgcgacgt ggtggatccg ccaggcaatc acgcgttcaa tcgccgacca ggcgcgcacc
     4021 atccgcatcc cggtgcacat gatcgagacc atcaacaaga tgaaccggat cagccgccag
     4081 atcctgcagg aaactggcac cgagcccgat cccgcgacgt tggcggtcaa gatggagatg
     4141 ccggaagaca agatccgcaa gatccttaag atcgcgaaag aaccgatcag catggaaacg
     4201 ccgatcggcg atgacgacga ctcgcacctc ggtgacttca tcgaggacca gaccacggtc
     4261 gcgccggtcg acgctgccca gtacaactcg ctctcgggcg tgaccaagga cgtgctggat
     4321 tcactgacgc cgcgcgaggc caaggtgctg cgtatgcgct tcggcatcga gatgtcgacc
     4381 gaccacacgc ttgaagaagt cggcaagcag ttcgacgtca cgcgcgaacg catccggcag
     4441 atcgaagcga aggcgctgcg caagctgcgc cacccgagcc gctcggacaa gctcaagagt
     4501 ttcctcgagt aggtcagagt gctgtcccaa tcaaaaacgc acggccccgg cccccgcagc
     4561 ggccgtgcac acttcggtcc gacgggccgt tagctcagct ggttagagca gaggactcat
     4621 aatcctttgg tcgctggttc aaatccagca cggcccacca gtaaacaaga gaacaaccgg
     4681 ccccaccctt gtccgactat cacgactttg atcacttcgg cctagacccg cgcatcgtcg
     4741 ccgcggtaaa ggccatgggt tacacgactc cgacgccgat ccagtcgaag gcgataccgc
     4801 tggtgatgca gggccgcgac gtgatgggtg ccgcgcagac cggaacaggg aagacggcgg
     4861 gcttcgcgct gccgatcatc cagcgcctgc tgccacagtc cagcaccagc atgtcgccgg
     4921 cgcgacaccc ggtgcgtgcg ctcgtgctca cgccgacacg tgaactggcc gaccaggtcg
     4981 ccgagaacgt caagctctac atcaccggga caccgttgcg ctgcgcggtc gtctacggcg
     5041 gcgtcgacat ggatccgcag acgcaggcgc tgcgcaccgg agtcgaaatt atcgtcgcca
     5101 cgccgggccg cctgctagac cacctgcaga gcaagaacac ctcgctcggc caggtgcaga
     5161 tcgtcgtgct cgacgaagcc gatcgcatgc tcgacatggg atttttgccg gacatctcgc
     5221 gcatcctgaa ccacctgcca aaagagcgac agagcctgat gttttcggcg acgttttccg
     5281 aggaaatcaa gaagctcgcg gccaacttcc tgcgcgatcc ggtgctggta gaagtggccc
     5341 gtcgcaacgc gaccgcagag aacgtcacgc aggaggtcgt caagctgcat gagtcggaga
     5401 agaccgatgt cctgctcgaa ctgctgcgca cgcgcggtga aggcggtggg cggctcacgc
     5461 aggtgctggt gttcgtcaac tcgaagatcg agtgccgtcg gctcgcccgc cagttgcaga
     5521 aggcggatgt caatgccgac gccatccatg gcgacaagac gcaggacgag cgtatgaagg
     5581 cgctcgaagg attcaagacc ggaacgatcg aagtgctggt cgccaccgat gtcgcggcgc
     5641 gcggactcga catcgtcgaa ctgccggtcg tcatcaacta cgacgtcccg ttttcgccgg
     5701 aagactatgt ccaccggatc ggcaggacgg gtcgcgcggg tgccacaggc atcgcgatca
     5761 tgatgatgac cggatcggat gagcggaatg tggcagcgat cgagcggctg accaaacaga
     5821 aattccaggc ggtctcggtt tctcccgagc gcggagagcg aagccctcag cgcagcgcat
     5881 cgcgtggcgg gcgggagcgt cccgaacggg acgacagccg gcgtggtcgc agcgaaccag
     5941 ccgtggaacc gcgccgtgcg ccgactcgac cggccgacga tttcttcagt cgaccctatg
     6001 aaccgggcgc ggcgggcgcc gagcccgcag taccgcctcc gaccgagtct gcgctgccgc
     6061 agcccgcccg gcccgggggt cgcgtggcgg cgctgctggg tggccgcaag aaatagtggg
     6121 catagctgga ccgcttgctc gaaacttgaa ccgcgatccg cctacagcag tgcgctgcta
     6181 gccaacgttg cattgatcct gcggtgaccg tgcaggaaac gtgatcacgt ggtcggcgtc
     6241 cagtcggatg ccaatcattt caccgatcgc gtggtcatgg tgactcggca ccagcgcgag
     6301 aacttcgcga ccgctctcaa ggcgcagcgt gtagatgaag tcgcctcccc ggaaaacctt
     6361 gcgcttgact tcggccttca gagggctggc atcgtcatgg atcacgtcgt ccggccgcaa
     6421 tagcacctcg acgtgacagc ccttgccaca ggtggcacac ccctgctgac acggcagcgg
     6481 aatctcgccc ctgagttcgc cgagttcgat ttccacattg cgcgcggaca gcacctgggc
     6541 cggcagaaac accccttgtc cgatgaagtc cgcaacgtag cgcgtcgcgg gccggtgata
     6601 cagctcgtac ggccggtccc actgctcgag gttgccgtcg tgcatcacgc cgacccggtc
     6661 tgcgatcgca aacgcctcct gctgatcatg cgtcactaga atcgccgtgg ttccgctcgc
     6721 cttcaggatc tggcgcacct cgtgcccgag acgctgccgc aactcgacat ccagactgga
     6781 aaatggctca tccatcagta gcagcgccgg cttcggtgcg agcgcccgtg ccaacgcgac
     6841 gcgctgctgc tgtccgcccg acaactcgtg cggataacgt tgggcttcgg atgcgagtcc
     6901 caccagcgcc accatctcat ccacgcgagc ggtgcgggcg ctcccgcgca gcgcacggag
     6961 accgaacgcg atgttgtcca cgaccttcag gtggggaaac agcgcatagt cctggaacac
     7021 cagtcctacc ccacgctttt caggcggagc cgtgaagtgc gggccggaaa cgaccagccc
     7081 atcgatttcg atcgtgccgg cgtcgaccgg caggaatccg gcaatggccc gcagcagcgt
     7141 cgtcttaccg caccccgaac tgccgaccag gcatccaatc tcgccgcgct ccagtgacag
     7201 cgtcagctgc tcgagcacgc gtgatccccc gcgataagtg accgcgaggt cgtcgatcac
     7261 cagcagggcg gggggcggcg gggcctgata catggcattc ctagtgtact ttaaatgcgg
     7321 ttagttctca tttgcattta gactccgcaa tcctaggagg gctattgagg accgagatcc
     7381 agaccaaccc cgcggatgcc cgggcacccg ttgcacggag gaggtctgct ccgatcgaat
     7441 cggcgctcgg ccgaccgttg ctgctggcgc tggtctgcac agcgttggtc ggtcttccgg
     7501 tgctcagcat cttcgccagt ttcgtcgaca cggcggcgtc ggcggaaacg ttgcgacacc
     7561 tggcggccac ggtgattccg gatgcgatgg tcgagacctt cgtgcttggc ggtggcgtcg
     7621 tgatcggcgt ggtgatcctt ggctcggcga ccgggtggat cgctgccagc tgcgagtttc
     7681 ccggtcgtcg cattaccgag tgggccctgc tgctgccgct ggcgatgccg gcctacatcg
     7741 tcgcctatgc ctacacggat gcactccagt tttccgggcc gatgcagagt gccctgcgcg
     7801 aaggctttgg ctggcgtcgc ggcgactact ggtttccgg
//
LOCUS       DATIRT010000526         2266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_1769422, whole genome shotgun sequence.
ACCESSION   DATIRT010000526 DATIRT010000000
VERSION     DATIRT010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1769422"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /gene="paaK"
                     /locus_tag="VLN25_12105"
     CDS             complement(<1..1208)
                     /gene="paaK"
                     /locus_tag="VLN25_12105"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HSQ11335.1"
                     /translation="MPVKNPDPADLEPIERAPRAELQSIQLERLRWSLAHAYEHVPHY
                     RARFDAAKVKPSDLKSLADLSRFPFTTKKDLRDNYPFGMFAVPREKVVRLHASSGTTG
                     KPTVVGYTAKDIDTWATVMARSIRAAGGRAGDMVHVSYGYGLFTGGLGAHYGAEKLGC
                     TVIPISGGMTERQVQIIADFRPDIIMVTPSYMLAIAEEMEKQGMDPKKSSLQIGIFGA
                     EPWTPAMRAAIEAKTSLDAIDIYGLSEIIGPGVAQECIETKDGLTVWEDHFYPEIVNP
                     DTGEVVPEGEKGELVFTSLTKEALPMIRYRTRDLTRLLPPTARSMRRMEKITGRSDDM
                     LIIRGVNVFPTQIEELILKEAELVPHYQLEITRPKNLDEMAVLVERSPAIGGADGDAA
                     GARLAHMVKS"
     gene            complement(1224..1670)
                     /gene="paaI"
                     /locus_tag="VLN25_12110"
     CDS             complement(1224..1670)
                     /gene="paaI"
                     /locus_tag="VLN25_12110"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HSQ11336.1"
                     /translation="MDDRKQHDPHAIAEAVKLGMFMDDAASRGLGMQIEAVGPGYARI
                     AMTVRPDMLNGFKICHGGFITTLADSAFAFACNSYNELTVASGIVVDFLAPAHEGDRL
                     TAECHEVALTGRTGVYDIKVTNQDGKAVVVMRGRSHTMKGKHVVQL"
     gene            complement(1663..>2266)
                     /locus_tag="VLN25_12115"
     CDS             complement(1663..>2266)
                     /locus_tag="VLN25_12115"
                     /inference="COORDINATES: protein motif:HMM:NF012928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HSQ11337.1"
                     /translation="LGIATPIIARLERAGIDVVRVQGPRAAQEVSSGWLEIGAARLMM
                     TDGRPATRRAMEDQHEHLVLFDLALDFATTPRLGLAPADQCGQAAFRSVCATLSGAGF
                     ACSPLDDVAGLVVLRLIAMLVNESADAVAYGICSPEAIDVAMRYGVNYPRGPMEWSEQ
                     VGLAVFARALANLREHYGEERYRTSSLIARKGYTGAGFHG"
BASE COUNT          368 a          774 c          716 g          408 t
ORIGIN      
        1 atcgacttga ccatgtgcgc aagccttgcg cccgcggcgt cgccgtcggc accgccgatt
       61 gccggcgaac gctcgaccag caccgccatc tcgtccagat tcttcggccg cgtgatctcg
      121 agctggtaat gcggcacgag ctcggcttcc ttcaggatca gctcctcgat ctgggtcgga
      181 aagacgttga cgccgcggat gatcagcatg tcgtccgacc gtcccgtgat tttctccatc
      241 cggcgcatcg accgcgccgt cggcggcagg agccgcgtga gatcgcgcgt gcggtagcgg
      301 atcatcggca acgcttcctt ggtgagcgag gtgaagacca gctcgccctt ctcgccctcc
      361 ggcacgactt cgccggtatc cgggttgacg atctcgggat agaagtgatc ctcccagacc
      421 gtgagcccgt ccttggtctc gatgcactcc tgcgccaccc ccggaccgat aatttccgac
      481 aggccgtaga tgtcgatcgc atcgagcgag gtcttggcct cgatcgccgc ccgcatcgcc
      541 ggcgtccagg gctcggcgcc gaagatgccg atctgcaatg acgacttttt cgggtccatg
      601 ccctgctttt ccatctcctc ggcgatcgcc agcatgtacg acggcgtcac catgatgatg
      661 tccggcctga agtccgcgat gatctgcacc tgccgctcgg tcatgccgcc cgagatcgga
      721 atcactgtgc agccgagctt ctccgcaccg tagtgcgcac cgagcccgcc ggtgaaaagc
      781 ccgtagccgt acgacacgtg caccatgtcg ccggcgcgac cgcccgcggc acggatcgag
      841 cgcgccatca ccgttgccca ggtgtcgatg tctttggccg tgtagccgac cacggtcggc
      901 ttgccggtcg tgccgctgga cgcatgcaag cgcacgacct tctcgcgcgg cacggcaaac
      961 atgccgaagg ggtagttgtc gcggagatcc ttcttcgtcg tgaacggaaa tctcgacagg
     1021 tccgcgagcg acttcaggtc cgacggtttc accttcgcgg cgtcgaacct ggcgcggtaa
     1081 tgcggcacgt gctcgtatgc gtgcgcgagc gaccagcgca ggcgttcaag ctggattgac
     1141 tgcagctcgg cgcgcggagc gcgctcgatc ggctcgaggt cggcgggatc ggggttcttg
     1201 acgggcatcg tgttggtccg cgctcagagt tgcacgacgt gcttgccctt catcgtgtgc
     1261 gagcgacccc gcatcacgac gacggccttg ccgtcctggt tggtgacctt gatgtcgtac
     1321 acgccagtac gccccgtcag cgcgacctcg tgacactcgg ccgtcagtcg gtcgccttca
     1381 tgcgcgggcg cgaggaaatc gacgacgatg ccggatgcga ccgtgagttc gttgtagctg
     1441 ttgcaggcga acgcaaaggc cgagtccgcc agcgtcgtga tgaagccgcc gtggcagatc
     1501 ttgaacccgt tcagcatgtc gggccgcacg gtcatggcaa tgcgtgcata gccgggcccg
     1561 accgcctcga tctgcatgcc gagcccgcgc gaggcggcat cgtccatgaa catcccgagc
     1621 ttcaccgctt cggcaatcgc gtgcggatca tgctgctttc tgtcatccat ggaatccggc
     1681 tcccgtgtaa cccttgcgtg cgatcagcga cgatgtccga tagcgctcct cgccatagtg
     1741 ttcgcgcagg ttcgcgagtg cacgcgcgaa aaccgcgagc cccacctgct ccgaccactc
     1801 catgggtcca cgcgggtagt tgacgccata gcgcatcgcc acgtcgatcg cttcggggct
     1861 gcagatgcca tacgccaccg cgtcggcaga ctcgttgacc agcatggcga tcagccgcag
     1921 caccaccagt ccggcgacat cgtcgagcgg cgaacacgcg aagccggccc cgctgagcgt
     1981 cgcgcagacc gagcgaaaag ccgcctggcc gcactggtcg gcaggcgcca gtcccaggcg
     2041 cggcgtggtc gcgaaatcaa gcgccagatc gaacagtacg aggtgttcat gctggtcctc
     2101 catcgcccgg cgtgtcgccg ggcgtccgtc ggtcatcatc aggcgcgcgg cgccgatctc
     2161 cagccagccg gacgaaacct cctgcgcggc gcgcgggccc tgcacgcgca cgacgtcgat
     2221 gccggcgcgt tccagccgcg cgatgatcgg cgtggcgatg ccgagc
//
LOCUS       DATIRT010000527         4437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_982260, whole genome shotgun sequence.
ACCESSION   DATIRT010000527 DATIRT010000000
VERSION     DATIRT010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982260"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..1436)
                     /locus_tag="VLN25_12120"
     CDS             complement(126..1436)
                     /locus_tag="VLN25_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSQ11338.1"
                     /translation="MPIFDPAALNPGVRPREVMAWASYDFANSGYTTVVLTAVFNAYL
                     VGVVAANADWATFAWTLTLGISNALVMLSMPMIGAYADLRARKKWLLAVSTGGCIAGT
                     AALAAVGPGDVALAAAAITVSNFFFMIGVALIAAFLPELARSDSLGKVSGWGWGFGYI
                     GGLITLALCLAYVQWAQAQGATAAEFVPITMLITATVFALAAAPALLIVKERAVPQMD
                     ARVGHFVHQALGRLLDTLRHIDRYRDFARLLVCGVFYQAGVMVVIALAAIYAQEVMKF
                     TFVQTMMLVLVVNVTAAIGAFAFGYVQEALGHKLALGLTLLMWLAMVTVAGLATSTAM
                     FWVAANLAGLAMGSSQSAGRALAGLFAPRDRLAEFYGLWTFATQLAAIIGPITYGAVV
                     YATDNNHRLAIGLTGLFFVAGLLALATIDIARGRRAAEASAAVP"
     gene            1519..1992
                     /locus_tag="VLN25_12125"
     CDS             1519..1992
                     /locus_tag="VLN25_12125"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HSQ11339.1"
                     /translation="MATKELDRIDRNILEILQADGRITNQELSERIALSPRACLDRVR
                     RLERAGLIAGYMAVLDPRKLGGLLTIVVEVTLKDQTQATHSRFELRMRLAEEVVECFL
                     VSGPCDYVLRLACRDLDHYRDLTNAWTDDPALGVEKISSKPELQTIKAFRGYPLA"
     gene            2158..3417
                     /gene="rocD"
                     /locus_tag="VLN25_12130"
     CDS             2158..3417
                     /gene="rocD"
                     /locus_tag="VLN25_12130"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HSQ11340.1"
                     /translation="MDARLGQVFNGSDQSIALEKSYAAPNYAPIPVVLERGEESWLWD
                     ADGKRYLDFMSAYSAVSHGHAHPRLVRALIEQAQRVAVTSRAYHSAVLGPFVAKLVEL
                     ADLRSPARALPASGGCESVETAIKAARRWGYRVKGVPADHAEIVVARGNFHGRSTSVV
                     GFSTEASYRADFGPFAPGFRHFDFGNLASLDAAINDHTCAVLIEPIQGEAGIVLPPDG
                     LLQSARRLCDERNVLLILDEIQTGLGRTGAMFAYMHEDIRPDGVLVGKALGGGLLPVS
                     AFVARAEILDLMGPGSHDSTFGGNPLAARVALEALRVLVDERLVERSHELGLHLLSRL
                     RGLDSTLIRSVRGRGLWVGVELDPMCVSARAVVERMAERGVLSKDTHETVIRFAPPLT
                     ISREALDWGIGVFASVLAEFETRPVTQ"
     gene            3604..4398
                     /locus_tag="VLN25_12135"
     CDS             3604..4398
                     /locus_tag="VLN25_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11341.1"
                     /translation="MEKLMRALSPSDGSADAVREAADAALMHGDNRPALERELDRLNT
                     AIQGSAAALGQHLSWTIVAQSFLLTAWVVVLVGSWSLPIPGKRWLLTGIAVFALAAVV
                     LTYLGLRAARDRVGPLKLQRQRVEDALERVAARPPAFARQGAITALLAQASTRGLPLL
                     IIGGWVALTLYTLALPLPTDSRTTSASPEPRPTANGMATAAPKSARPGPATAAAKATA
                     APAEAAPAVTPATSADVSGESPLLNFFRRAVNTPPAAEPQIESIRP"
BASE COUNT          784 a         1491 c         1426 g          736 t
ORIGIN      
        1 ggtgtttcgg gcgtgaagtc ggtcgaggac gcacagatcg ccaaggcggg catcgcggcg
       61 ctgtaggccg tcgctagacc tgcgcagagc gcaggatcag cacacagtct caggacagga
      121 atgcgtcagg gaaccgctgc cgatgcctcc gccgcgcggc ggcctctcgc aatgtcaatt
      181 gtcgccagcg cgagcaggcc ggcgacgaag aacagtcccg tgagcccgat cgccagccga
      241 tgattgttgt ccgtcgcata gaccaccgcc ccataggtga tcggaccgat gatcgcagcc
      301 agttgcgtcg cgaaagtcca tagtccgtag aactcggcga ggcggtcccg tggggcgaag
      361 agtcccgcca acgcgcgtcc ggccgactgg ctcgatccca ttgccagtcc cgccaggttg
      421 gccgccaccc agaacatcgc cgtgctggtc gccagtcccg ccaccgtcac catcgccagc
      481 cacatcagca acgtgaggcc gagggcgagc ttgtgcccga gggcttcctg cacatagccg
      541 aaggcgaacg cgccgatcgc ggccgtgacg ttcacgacca gcaccagcat catcgtctgc
      601 acgaacgtga acttcatcac ttcctgcgcg tagatcgccg ccagcgcgat gacgaccatg
      661 acgccggcct ggtagaagac cccgcagacg agcagtcggg cgaagtcgcg atatcgatcg
      721 atgtggcgca gcgtgtcgag cagtcgcccc agcgcctggt gcacgaaatg gccgacgcgt
      781 gcatccatct gcggtactgc gcgctccttg acgatcagca gtgccggtgc cgcggccagc
      841 gcaaataccg tggcggtgat gagcatcgtg atcggcacga actcggcagc ggtcgcgcct
      901 tgagcctggg cccactgcac gtaggccagg cataacgcca gtgtgatgag gccgccgata
      961 tagccgaagc cccaacccca gccggagacc ttgccgagcg aatcagaacg ggccagctcc
     1021 ggcaggaacg ccgcgatcaa cgccacgccg atcatgaaga agaagttcga caccgtgatg
     1081 gccgcggcgg ccagcgcgac atcgcccggc ccgacggccg caagcgcggc agttccggca
     1141 atgcagccac cggtcgacac ggccaacaac catttcttgc gcgcgcgcag atcggcatag
     1201 gcgccaatca tcggcatcga cagcatcacg agtgcattgg agattccgag cgtcagcgtc
     1261 caggcaaagg tcgcccagtc ggcgttggcc gcgacgacgc cgactaagta ggcgttgaac
     1321 actgccgtca gcaccaccgt cgtgtagccc gagttggcga agtcgtagct ggcccaggcc
     1381 atcacctcgc gcggacggac gcccggattg agtgctgcag gatcgaagat gggcatcgct
     1441 gacagaaagc tgaagtgcgt tggcgcatcc tacgtgaggc gatccgcctt ggtcgtgacc
     1501 gttcgtatac tgccgcgcat ggcgaccaaa gaactcgatc gcatcgatcg caacatcctg
     1561 gagatcctgc aggccgacgg ccgcatcacg aaccaggagt tgtcggaacg catcgcgctc
     1621 agcccgcgcg cctgcctcga tcgggtgcga cggctcgaac gcgcgggcct gatcgccggc
     1681 tacatggcgg tgcttgaccc gcgcaagctc ggcgggctgc tcaccatcgt cgtcgaagtg
     1741 acactgaaag accagacgca ggccacgcac agccgtttcg agctgcgcat gcgcctggcc
     1801 gaggaggtcg tcgaatgttt cctcgtgtcc ggcccctgcg actacgtgct gcggctcgcc
     1861 tgccgcgatc tcgatcacta tcgcgacctc acgaatgcgt ggaccgacga tccggcgctt
     1921 ggtgtagaaa agatcagttc aaagccggaa ctgcagacga tcaaggcctt tcgaggctat
     1981 ccgctggcct gatagacacc gaccccctcg gcatggagcg ctgccgaatc ggcggaagtc
     2041 gcacgaacat actgccgctt cgagcaaaac ggctagcgtc aatctgcctc gccctgcttt
     2101 tgcgccacat acactggctg gagtttcagc gaggtggcag tttcaacgag gtggaccatg
     2161 gatgcgcggc tcggacaggt tttcaatgga tcggaccagt cgattgcgct ggaaaaatcg
     2221 tacgcggcgc cgaattacgc gccgatcccg gtcgtgctcg aacgtggcga ggaaagctgg
     2281 ctctgggatg ccgacggcaa gcgctatctc gatttcatgt ccgcgtattc cgcggtcagt
     2341 catggccacg cgcatccacg gctggtccgg gcgctgattg aacaggcgca gcgcgtggcg
     2401 gtcacgtccc gtgcctatca ctcggccgta ctcggtccgt tcgtggcaaa gcttgttgag
     2461 cttgcggatc tgcgctcgcc ggcgcgcgct ctgcccgcga gcggcggctg cgaatcggtc
     2521 gaaaccgcga tcaaggcggc gcgccgctgg ggctatcgcg tcaagggcgt tcctgccgac
     2581 cacgccgaaa tcgtcgtcgc gcgcggcaac ttccatgggc gctcgaccag cgtggtcggc
     2641 ttctcgacgg aggcgtcgta tcgcgcggat ttcggaccgt ttgctcccgg cttccggcat
     2701 tttgacttcg gcaatctcgc gtcactcgat gcggccatca atgatcacac ctgtgccgta
     2761 ctgatcgaac cgatccaggg cgaagccggc atcgtgttgc cgcccgacgg tttactgcag
     2821 tccgcgcgca gactgtgcga tgagcgcaac gtgctgctga tccttgacga gatccagacc
     2881 gggctcggtc gtaccggcgc gatgttcgcc tacatgcacg aagacattcg tcccgatggc
     2941 gtgctggtcg gcaaggccct cggcggtggg ctgctcccgg tatcggcgtt cgtcgcccgt
     3001 gccgagatac tggacctgat ggggccgggc agccacgact cgaccttcgg tggcaatccg
     3061 ctcgctgcgc gggtggcgct cgaagcgctg cgcgtcctcg ttgacgagcg actggtcgaa
     3121 cgcagccacg agctcggctt gcatttgttg tcacgcctgc gcggactcga ctccaccttg
     3181 atccgctccg tgcgcggacg cggcctgtgg gtcggcgtcg agctcgaccc catgtgcgtg
     3241 tccgcgcgcg cggtcgtcga gcgcatggcc gagcgcggcg tgctgagcaa ggacacgcac
     3301 gagaccgtga ttcgctttgc cccgccgttg acgatcagcc gcgaggccct ggactggggc
     3361 atcggcgtct tcgcgtccgt gctggccgag ttcgaaacgc gtcccgtcac gcagtagtgt
     3421 caggcttcca cgcgcatctg cgctgcgctt tctctttacg gcagtatcga ccgaagcctc
     3481 ggcgggcgcg tgcccgaacg cgacgcaagc gccaaccatc gcccttggaa gtgagtcgcc
     3541 ctcccccgga cgggcgatcc cccgcgctgc actcgctgtc gaaactccat gcaggatctg
     3601 gcaatggaga aactcatgcg agcactatca ccgagcgatg gcagcgccga cgcggtccgg
     3661 gaagcggcgg acgcagcgtt gatgcacggg gacaaccggc ccgcgctcga acgcgagctg
     3721 gatcggctga acaccgcgat tcaaggctct gcggccgcgc tcgggcagca tctgtcatgg
     3781 acgatcgttg cccagtcgtt cctgctgacc gcctgggtgg tcgtcctggt cggctcctgg
     3841 tccttgccga ttccgggcaa gcgctggcta ctgaccggga tcgcggtctt tgcactcgcc
     3901 gccgtcgtgc tcacctatct cggactgcgc gccgcacgtg atcgggtcgg cccgctcaaa
     3961 ctgcaacgcc aacgcgtcga agacgccctc gaacgcgtgg ccgcacgtcc tcccgccttt
     4021 gctcgccagg gagcgatcac ggcgctgctg gcacaggctt caacgcgcgg cctgccgctg
     4081 ctgatcatcg gcggatgggt cgcactgacg ctatacacgc tggcactgcc actgccgacc
     4141 gacagcagga cgacgtcggc atcgcccgaa ccgcgcccga ccgcgaatgg aatggcgacc
     4201 gctgcgccga agtcggcacg acccggcccg gccactgcgg ccgcgaaagc cacggcggcg
     4261 cctgccgaag cagcgcctgc ggtaacgccg gccaccagtg cagacgtcag cggcgaatca
     4321 ccgctgctca atttcttccg ccgcgcggtc aacacgccgc cggcggcgga gccgcagata
     4381 gagtcgatca ggccctagga tcgcgcagcg cgcatcaagg cgcccaggtc ggcttgc
//
LOCUS       DATIRT010000528         3075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_688087, whole genome shotgun sequence.
ACCESSION   DATIRT010000528 DATIRT010000000
VERSION     DATIRT010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688087"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="VLN25_12140"
     CDS             complement(<1..892)
                     /locus_tag="VLN25_12140"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HSQ11342.1"
                     /translation="MSGLAERAFALRRTAAIAGARPGTFLLNILLCAAGLAIPLFIAA
                     LLHAATPWAQRISAGPEISVFAALGSSRTDLDSLKGKLAAIEGVAGVRLIPRDLALAE
                     LSRRAGLAGAADTRANPLPDVLVAHFNIRTQPGVVERAATAIKGWAGVDAVQSDIEWH
                     RRLAAIGQALVAVLAAVGALVLVLIILVLVTAAQSQVRLRHDETAVLQLVGARPSFIV
                     RPFAYASALTLGLGAMLSLALAVLAVRLIEPRVAALAAVFGQSLDWPSLPLGFALAFV
                     AAAAVVGWAAGWVGASNAARR"
     gene            complement(889..1569)
                     /locus_tag="VLN25_12145"
     CDS             complement(889..1569)
                     /locus_tag="VLN25_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSQ11343.1"
                     /translation="MNAIPTLAAPLIVFDRVGKRYGDKTVVDGLSFTISRNEFVLLSG
                     PSGAGKSTVIRLIAALELPTTGIIRVAGESLTHMKRRALPHLRRSIGIVLQKLMLLDD
                     RTILQNVALPVVAAGFRWAEARTRAAAALQRVGLDEDAGRAQPRELSGGAQQRAALAR
                     ALVNRPTLLLVDEPTAHLDRTAAAEVMRLLEQFVIAGVTVIMATHGETASVPARARII
                     GLDVVPST"
     gene            1746..>3075
                     /gene="ppk1"
                     /locus_tag="VLN25_12150"
     CDS             1746..>3075
                     /gene="ppk1"
                     /locus_tag="VLN25_12150"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HSQ11344.1"
                     /translation="MQTSPELFLNRELSMLAFNERVLAMATRTSVPLAERLRYVCIVS
                     SNLDEFFEVRISDLSEEIRETGGASPQATAYAEIMQRAHQLVHEQYLLFNQTIMPALE
                     RRDIVVLNHAQRDESQRAWVREYFMREVRPLLSPIGLDPSHPFPQVLNTSLNFILQLE
                     GKDAFGRPSATAILKVPRVLPRVIPLPARLVPGKQAFVMLSSMIRAHLQDVFPGREIG
                     GFSQFRVTRDSDLSVDEREVHNLRQAMRMELTQRHFGNAVRLEVLASCPPPLQALLQE
                     QFQLPEQAVFRVDGPVNLVRLNQLVDHLTQPPLRWAPFVPAWPTKLASGSDMFEAIRR
                     SDILMHHPFESFEPVIEFLRQAAVDAKVVAIRMTLYRTGISSVPVDILEEAARRGKEV
                     TVIVELKARFDEEANINRAERLEAVGAQVVYGVVGLKTHGKMTLVMRREEV"
BASE COUNT          553 a         1018 c         1017 g          487 t
ORIGIN      
        1 atcttcgtgc ggcgttgctg gccccaaccc accccgcggc ccagcccacg acggccgccg
       61 ccgcaacgaa tgcaagggcg aaaccgaggg gaaggctcgg ccagtcgagc gattgcccga
      121 agacagcggc gagagcggcg acgcgaggtt cgatcaaccg aaccgccagg acagcaagcg
      181 ccagcgacag catcgcgccg agtcccaggg tcaatgcact cgcataggca aagggacgaa
      241 cgatgaacga aggccgcgcg cccaccagtt gcagcacggc cgtttcgtcg tgccgcaagc
      301 ggacctgcga ctgcgccgcc gtcaccagca cgagaatgat caggacgagg accaatgcgc
      361 cgacggcagc cagcactgcg accagcgcct ggccgatcgc tgccagccga cggtgccact
      421 cgatgtccga ctggaccgca tcgacgccgg cccatccctt gattgctgtg gcagcgcgct
      481 cgaccactcc cggttgggtc ctgatgttga agtgggccac caggacatcg ggcaacggat
      541 tcgctcgcgt gtcggccgcg cccgccagcc cggcccgccg gctcagttcc gccagagcca
      601 ggtcgcgagg aatcagacgc acgccggcca cgccttcgat ggcagcgagc tttcccttca
      661 acgaatcgag gtcggttcgc gaactgccga gcgcggcaaa gacgctgatc tcggggccgg
      721 cggagatgcg ctgggcccac ggagtcgccg cgtgtagcaa ggcggcaatg aacaggggaa
      781 tggccaggcc tgcagcgcag agcaggatgt tgagcaggaa agtgcccgga cgtgcgccgg
      841 cgatggcggc tgtacgccgc agcgcaaacg cgcgctcggc cagtccactc atgtcgaagg
      901 caccacatcg agcccaatga tgcgggcacg ggcaggcacc gaggccgtct cgccatgcgt
      961 ggccatgatc accgtgaccc ctgcaatcac gaactgttcg agcagccgca tcacctcagc
     1021 cgcggcggtg cggtcgagat gtgcggtggg ttcatcgacc agcagcagcg tgggccgatt
     1081 gacgagcgcg cgcgcgagag ccgcgcgttg ctgcgcgccg ccggacagtt cgcgcggctg
     1141 cgcccggccc gcgtcctcgt ccagaccgac gcgctgaagc gccgcggccg cccgggtcct
     1201 cgcttcggcc cagcggaatc cggccgcgac aacgggcagg gcgacgttct gaaggatcgt
     1261 tcgatcatcc agcagcatca gcttctgcag cacgatgccg atcgatcggc gcagatgcgg
     1321 caatgctcgc cttttcatgt gcgtcagcga ctcaccggcc acgcggatga tgccggtggt
     1381 tggaagttcg agtgcggcga tgagccggat cacggtcgac ttgcccgcgc cggaggggcc
     1441 tgagagcaac acgaactcgt tgcgtgagat cgtgaaactc aacccgtcga cgaccgtttt
     1501 atcgccgtag cgcttgccga cgcggtcgaa gacgatcagc ggcgcggcga gtgtcggaat
     1561 ggcattcata cggcggggct cgggcaagga ggcgccgctt agaatgagtc gaaccctgag
     1621 ttgagcgctg aaacgacgcc ggcggtcctg gccatcccgg cgattgtacg gttgtcatcg
     1681 aagcgtcatc gcgaatcact atggtcagtc accacaagcg caagcggccc cggcgaaagt
     1741 tcgaattgca gaccagtccc gagctcttcc tgaaccggga gctgtcgatg ctcgcgttca
     1801 acgaacgcgt gctggctatg gccacgcgca cctcggtgcc gctggccgag cgtctgcgct
     1861 acgtctgcat cgtctcgtcc aacctcgacg aattcttcga agtgcggatc tccgacctca
     1921 gcgaggagat ccgcgagacc ggcggcgcct cgccccaggc gactgcgtac gccgagatca
     1981 tgcaaagggc tcaccagctg gtgcacgagc agtacctgct cttcaaccag accatcatgc
     2041 ctgcgctcga acggcgcgac atcgtcgtgc tgaatcacgc gcagcgcgat gagtcgcagc
     2101 gcgcctgggt acgcgagtac ttcatgcgcg aggtgcggcc gctgctctcg ccgatcggcc
     2161 tggacccttc gcatccgttt ccgcaggtcc tgaacacatc tctgaacttc atccttcagc
     2221 tcgaaggcaa ggacgccttc ggtcgcccct cggccactgc gatcctcaag gtgccgcgcg
     2281 tgctgccgcg ggtgattccg ctgcccgcgc ggctggtgcc gggcaagcag gcgtttgtga
     2341 tgctgtcctc gatgatccgt gcgcatttgc aggacgtctt tccggggcgc gagatcggcg
     2401 gcttttcgca gttccgcgtg acgcgcgact ccgacctcag tgtcgacgag cgcgaggtgc
     2461 acaacctgcg ccaggcaatg cgcatggaac tgacccagcg gcacttcggc aatgcggtcc
     2521 ggctcgaggt gcttgcatcc tgcccaccgc ccctgcaggc gctgctgcag gaacagttcc
     2581 agttgcctga gcaggcggtg tttcgcgtcg acgggccagt caacctggtg cgcctgaatc
     2641 agctggtcga tcacctgacc caacccccgc tgcgctgggc gccgttcgtg ccggcttggc
     2701 cgacgaaact ggcgagcggc tccgacatgt tcgaggcgat ccggcgcagc gatatcctga
     2761 tgcaccaccc gttcgagagt ttcgagccgg tcatcgaatt cctgcgccag gcggcggtcg
     2821 atgccaaggt cgtggcgatc cggatgacgc tgtaccgcac cggaatcagt tcggtgccag
     2881 tcgacatcct cgaggaagcg gcgcgccgcg gcaaggaggt cacggtgatc gtcgagctga
     2941 aggcacgctt cgacgaggag gccaacatca atcgggccga acgtctcgag gctgtcggcg
     3001 cgcaggtcgt ctacggcgtg gtcggtctga agacccacgg caagatgacg ctggtgatgc
     3061 gccgcgagga ggtgc
//
LOCUS       DATIRT010000529        13865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U15910
            k141_294676, whole genome shotgun sequence.
ACCESSION   DATIRT010000529 DATIRT010000000
VERSION     DATIRT010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936620
            Sequence Read Archive: SRR9691031
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 08:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,448
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,372
            CDSs (with protein)               :: 2,372
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 4 of 40
            Pseudo Genes (incomplete)         :: 15 of 40
            Pseudo Genes (internal stop)      :: 22 of 40
            Pseudo Genes (multiple problems)  :: 1 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13865
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294676"
                     /isolate="SMAG_U15910"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.6 N 100 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..315
                     /gene="rpsK"
                     /locus_tag="VLN25_12155"
                     /pseudo
     CDS             1..315
                     /gene="rpsK"
                     /locus_tag="VLN25_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106947.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
     gene            485..1108
                     /gene="rpsD"
                     /locus_tag="VLN25_12160"
     CDS             485..1108
                     /gene="rpsD"
                     /locus_tag="VLN25_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HSQ11345.1"
                     /translation="MARYTGPKLTLSRREGTDLFLKSSRRAFDSKAKADSKPGQHGRT
                     SGARQSDYGTQLREKQKVKRMYGVLERQFRRYYQEASRRKGNTGEHLIMLLESRLDNV
                     VYRMGFGATRAEARQLVSHRAIALNGETAGIPSMLVKSGDIITVREKAKKQGRIQEAL
                     TLAEQSGFPSWVEVDPKKLEGKFKQLPDRGEVAQDVNESLVVELYSR"
     gene            1243..2226
                     /gene="rpoA"
                     /locus_tag="VLN25_12165"
     CDS             1243..2226
                     /gene="rpoA"
                     /locus_tag="VLN25_12165"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HSQ11346.1"
                     /translation="MTSTALLKPRAIEVEAVGPNAAIVTMEPFERGYGHTLGNALRRI
                     LLSSMVGYAPTEAQITGVVHEYSTVDGVREDVVDILLNMKGIVFKLHNRDEVTLTLRK
                     EGEGVVIASDIELPHDVEIVNPDHVIAHLSGGGRLELQVKVEKGRGYVPGNVRAFRDD
                     HSKTIGRIVMDASFSPVKRVSYAVEAARVEQRTDLDKLVMTIETNGAMTPEEAVRQSA
                     RILMDQLQVFGALEGMAETSAAASSRSPQVDPILLRPVDDLELTVRSANCLKAENIYY
                     IGDLIQRTENELLKTPNLGRKSLNEIKEVLAARGLTLGMKLENWPPAGLDK"
     gene            2345..2740
                     /gene="rplQ"
                     /locus_tag="VLN25_12170"
     CDS             2345..2740
                     /gene="rplQ"
                     /locus_tag="VLN25_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HSQ11347.1"
                     /translation="MRHGHGLRKLNRTSSHRLAMLRNMTNSLLRHEAIKTTVPKAKEL
                     RRVVEPLITLGREPTVANKRLAFNRLRDREMVVKLFNEIGPRYKTRNGGYTRILKMGF
                     RVGDNAPMAFMELVDRPAPLVKEESADAS"
     gene            2817..3767
                     /locus_tag="VLN25_12175"
     CDS             2817..3767
                     /locus_tag="VLN25_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSQ11348.1"
                     /translation="MDAAVVPALNLDEVLDVRDLRKVYGGAPVVDGLSFSIRRGECYA
                     LLGPNGAGKTTTLRCCLGLTRPDGGTIRLVGEPVPREARRARIRVGVVPQIDNLDPDF
                     TVTENLVVYGRYFGLTDRDVAQRLPALLEFASLAHKKDARIGELSGGMKRRLTLARSL
                     VNDPDLIFMDEPTTGLDPQARHLIWDRLKQLLGRGKTILLTTHFMDEAERLANRLAVI
                     DHGRMIAEGAPRDLIHEHIEPQVVEIYGDGARQWAEHVGRALLGRLEITGETVFCYSH
                     DPAPVLHALESNVGLRYLHRPANLEDLFLKLTGREMRDES"
     gene            3764..4576
                     /locus_tag="VLN25_12180"
     CDS             3764..4576
                     /locus_tag="VLN25_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSQ11349.1"
                     /translation="MSASVPATSTGSSVWAPPRLSWRFVPVWRRNFLVWRKLFVERLL
                     TNVVEPLLTIAAFGYGLGSLLPEVDGVSYLQFLASGSIAMSVMYSAKFESLWGAFSRM
                     SIQKTWDAILHTPLAVDDLLLGELVWAASKATFTAVCILAMIWLLDIAREPLSLLVLP
                     TAFFIALVFAAMALIVNAVARSYDLLSNYFTVVVMPLVFLSGVYFPLTQLPNWLRTLA
                     EWLPMTAAVNLVRPLALGRWPTNGLSDVLLLALWGIACYTVALGLTRKRLLK"
     gene            4576..4908
                     /gene="cutA"
                     /locus_tag="VLN25_12185"
     CDS             4576..4908
                     /gene="cutA"
                     /locus_tag="VLN25_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HSQ11350.1"
                     /translation="MDNVLIVLTNCPDVGVADRIARTLVEQRLVACANRLAEVDSIFR
                     WQGAVERAVEVPLLIKTTRERYSEVEAAIRALHPYDVPEIIAWPVTAGLPAYLRWVVE
                     ETQPPLQA"
     gene            4988..5398
                     /locus_tag="VLN25_12190"
     CDS             4988..5398
                     /locus_tag="VLN25_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HSQ11351.1"
                     /translation="MAASDTLDAIRFDKWLWAARFFKTRSLASEAVERGQVRVDDQRI
                     KPARTARVGDRVVIQRGDDRIEFVVRALSAVRGPAPVAQRLYDETPESRTRREQASVQ
                     RRLARDPAAMIKGRPSKRDARALRRMTGTPDMDR"
     gene            5417..6073
                     /locus_tag="VLN25_12195"
     CDS             5417..6073
                     /locus_tag="VLN25_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11352.1"
                     /translation="MKLRIPVALTCMLMAPLHASAIPPSVPMSGFAKYEFNKWLFRSC
                     TGSGKTSTLQAQGLPFIDATPERVLFAAIQQRWKQSADPLRGVYLEFAGFTENGCVTA
                     TDMQRSLGWVESCAQRPSNIAPGARLWAAGNEPGWNFVVDGKTAKFRTPDAVLNFPVV
                     AAKSSDGTVTYVADSAGDRIRVELTHGLCNDTMSEAAFGRRAVVAVRGALYTGCGLVR
                     "
     gene            complement(6074..6835)
                     /locus_tag="VLN25_12200"
     CDS             complement(6074..6835)
                     /locus_tag="VLN25_12200"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HSQ11353.1"
                     /translation="MTFFLLERLLVTVRQGSSKTVEQVRQRLVDPRMRTDPSPYDKSR
                     LPKSPGELMMRLLNGMVDRYLELREPLSDRLDRWQRDLLDPRRPFSNWSALLEARIEL
                     RKLENLCEGQYDALTELRDSYLEETPDAAINDAYLVRLNDVLEHIQRVLHHARRLETS
                     AESAVQLHFSANTHQTNEIVRKLTAIAAIFAPLTLITGVFGMNFQHMPLLQELNGFWL
                     TITGMGSIIVVMLLYFWARRILDRPRTPRPPRWLR"
     gene            complement(6839..7177)
                     /locus_tag="VLN25_12205"
     CDS             complement(6839..7177)
                     /locus_tag="VLN25_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSQ11354.1"
                     /translation="MKWIEIGPQHATELAAEPARPPAEGFLWLDVLHDEAIADPEALR
                     EAVQRIFSVRLHDLHLQDAANLQHPSYFDSTKDYDMLVFRKLATGEFRPLEERAGPAD
                     QRRMLQEIVT"
     gene            complement(7188..8195)
                     /gene="hemB"
                     /locus_tag="VLN25_12210"
     CDS             complement(7188..8195)
                     /gene="hemB"
                     /locus_tag="VLN25_12210"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HSQ11355.1"
                     /translation="MQILGQFPAVRMRRMRRDDFSRRLMRENALTVDDLIYPVFVLDG
                     ARRREAVASMPGIERVSIDELLPVADECVRLGIPVIALFPVIDASLKSANGAEAANAE
                     GLVPRAVRTLKQHFPQLGLLTDVALDPFTTHGQDGLIDDAGYVLNDETIEVLVQQALV
                     QAAAGVDIVAPSDMMDGRIGAIRQALEGAGHIHTRIMAYSAKYASAFYGPFRDAVGST
                     SNLGKSNKMVYQMDPANTDEALWEVGLDLAEGADMVMVKPGMPYLDVVRRVKDAYRAP
                     TFVDQVSGEYAMLKAAAANGWLDEKKVVLESLLAFKRAGADGVLTYFALPVARWLKAE
                     R"
     gene            complement(8384..9004)
                     /gene="yihA"
                     /locus_tag="VLN25_12215"
     CDS             complement(8384..9004)
                     /gene="yihA"
                     /locus_tag="VLN25_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HSQ11356.1"
                     /translation="MSLFHSARFVTSVADLTAIAPPSLPEIAFAGRSNAGKSTVINVL
                     TQQKRLAFASKTPGRTQLLNFFSLSEKGEHGDRVDRGILVDLPGYGYAKADAGQRATW
                     DLLVGSYVRERQTLIGIVLVMDARRPFMVGDDALLRFVDRSGCLLHFLLNKSDQLGTM
                     EKRAALAAAQRRAEAIGARASAQLFSGLKREGIDDLTETLTRWLSA"
     gene            9201..9803
                     /locus_tag="VLN25_12220"
     CDS             9201..9803
                     /locus_tag="VLN25_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSQ11357.1"
                     /translation="MAWAPTVFAQAKSVKADAAKGQQIASQVCAACHTADGNSTIAAN
                     PKLAQQHTSYLHKQLVDYTVRAGEQKPARENAIMNGMAATLSDQDKRDVSAWYASQPA
                     KPGTARNKDTLELGQRIWRAGIPEKALPACSGCHNPAGAGIPNQYPRLAGQHAEYTEA
                     TLKSFREGTRRNNLPMQQIAARLSDAEVRAVADYIQGLRK"
     gene            complement(9840..10880)
                     /locus_tag="VLN25_12225"
     CDS             complement(9840..10880)
                     /locus_tag="VLN25_12225"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HSQ11358.1"
                     /translation="MHIDYQVKLDFKDVLIRPKRSTLTSRSDVDISREFTFRHAGVKY
                     HGIPIIASNMDTIGTFEMARALDTFRLSTALHKHYSVDDCVAFFRSLERKSAAFYSMG
                     ITKVDEEKFRRVMDAAADAVEYVCIDVANGYHENFVTFVTRIRTAYPRLVIMAGNVVT
                     GEMTEELILSGADIVKVGIGPGSVCTTRRMTGVGYPQLSAVIECADAAHGLSGLICSD
                     GGCTTPGDVAKAFGGGADFVMLGGMFAGHEESGGERLERDGASYQRFYGMSSKAAMER
                     YAGGVAGYRAAEGKEVLVPFRGPVASTAQEILGGVRSACTYVGARRLKEFSKRTSFVR
                     VTQQVNEVFGTT"
     gene            11034..11987
                     /gene="msrP"
                     /locus_tag="VLN25_12230"
     CDS             11034..11987
                     /gene="msrP"
                     /locus_tag="VLN25_12230"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HSQ11359.1"
                     /translation="MLIRHALPFSASEITPEGVVRQRREFLQVGVGGAFALMAPSLAN
                     AQVPSPAALAARPSPLSVMEKPTPANLVTSYCNFYEFGTEKDDPPQRAPQSLKTRPWT
                     VRVEGEVRKARTFDIDDLLALAPMEERVYRLRCVEAWSAVVPWIGYQLAELIRRVEPT
                     GNAKFVEFTTLADPKQMPGLRSSVIDWPYTEGLRLDEAMHPLTLLGFGMYGQTMPAQN
                     GAPVRLVVPWKYGFKSAKSIVLIRFTEKQPMIAWQKSNPAEYGFYSNVNPERDHPRWS
                     QKSERRLGEEIFSRRRPTLMFNGYGEQVASLYAGMDLKKFY"
     gene            11987..12661
                     /locus_tag="VLN25_12235"
     CDS             11987..12661
                     /locus_tag="VLN25_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013204967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HSQ11360.1"
                     /translation="MAGLSRAFRFVPPALPASVFAAVRAVVFTLAMLPLLALIYRATD
                     GGLGANPVEAILRSLGTWTLVLLLVTLAITPLRWLTGWAWLLRLRRMLGLTSFFYALL
                     HVVAFVWLDHWFEWAAIVADVVKRPYLTFGFAAFVLMIPLAATSTNAMVRRLGGRQWQ
                     QLHRLVYGIAVLGVLHFWFHKLAKNDLAEPALYAAVLAVLLGTRLIHWWVASRRPGGL
                     VPAVRR"
     gene            complement(12730..>13865)
                     /gene="lysA"
                     /locus_tag="VLN25_12240"
     CDS             complement(12730..>13865)
                     /gene="lysA"
                     /locus_tag="VLN25_12240"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HSQ11361.1"
                     /translation="YVSYAEALAGRASLVCYAMKANSNLAVLNVLARLGAGFDIVSGG
                     ELARVLAAGGAASKVVFSGVGKSEAEIEQALAVDILCFNVESAAELERIDAVACHLGR
                     RARISLRVNPDVDAGTHPYIATGLKDNKFGIDIDETLPLYRDAARRSGLEVVGIDCHI
                     GSQVTELAPYLDAADRVLDVVEALERDGIGLRHIDFGGGLGIRYRQESPPASGELVRA
                     LLQRVDARGFGAKTVMVEPGRSLVGHAGMLVTRVEYLKHGAAKNFAIVDAAMNDLLRP
                     TLYDAWMDVVAVEARSGPTQTYDIVGPICESGDWLARERALSLAPGDLLAIAGAGAYG
                     MAMSSNYNTRPRAAEVMVDGDRAFAVRRRETVAELFAGESTLP"
BASE COUNT         2477 a         4359 c         4534 g         2495 t
ORIGIN      
        1 tcgttcaaca acacgatcat cacgatcacc gaccggcagg gcaacgcgct gtcgtgggcc
       61 agttcgggtg gccagggctt caagggttaa cgcaagtcga cgccctttgc ggcccaggtt
      121 gccgcggaaa atgccggccg cgccgcccag gagcatggca tcaaggctct ggaagtccgc
      181 atcaccggac ccgggccggg ccgcgagtca tcggtgcgcg cgttgaacgc gctcggcatc
      241 cgtatctctt ctatccagga catcacgccg gttccgcaca acggcgtgcg tcctccgaag
      301 cgccgtcgcg tctagcgaga cgcgcgaaag tgcaaaccta ctttcgcgcg gaatagtttg
      361 aaaatttcgc tggcaaagcc agtcgaaatt tttcatcaca aaggcgggta gccgtctcgg
      421 agcgtcaccg acagcagatc ggtgacttaa tgcggcaagg ccgcaggatg aaaggaagac
      481 tgaagtggca cgttataccg gaccaaaact gacgctctcg cgccgcgagg gcaccgatct
      541 ctttctcaag agttcacgtc gcgccttcga ttcgaaggcc aaggcggaca gcaagccggg
      601 ccagcacggc cgcacctcgg gtgcgcgcca gtcggactac ggcacgcagc tgcgcgagaa
      661 gcagaaggtc aagcgcatgt atggcgttct cgagcgccag ttccgccgct attaccagga
      721 agcgtcgcgc cgcaagggca acaccggcga acacctgatc atgctgctcg agtcgcgcct
      781 ggacaacgtc gtctaccgca tgggcttcgg cgccacgcgc gcggaggcac gccagctggt
      841 aagccaccgc gcgatcgcgc tcaatggcga gacggccggc attccgtcga tgcttgttaa
      901 gtcgggcgac atcatcacgg tccgggagaa ggccaagaag cagggccgca tccaggaagc
      961 gttgacactg gccgagcagt cgggctttcc gtcctgggtt gaagtcgacc ccaagaagct
     1021 ggaaggcaag ttcaagcagt tgcctgatcg cggcgaagtg gcgcaggacg tcaacgagtc
     1081 gctggtcgtc gagttgtatt cgaggtagta gtccggggat gccggcccgc atgcgcggtc
     1141 cggcgacgaa agcgggttgg gcaaccgcgt tcgttacttc tttgccctaa ccatcagcct
     1201 tatcggcgta acgagccgag ggtattgaag ggaaagtctc aaatgacaag cactgcactt
     1261 ctcaagccgc gcgcgatcga ggtcgaggcg gttggcccga acgcggccat cgtcacgatg
     1321 gaaccgttcg agcgcggcta cggccacacg ctcggcaacg cgctgcgtcg tatcctgctg
     1381 tcgtccatgg tcggctacgc gcccaccgag gcacagatta ccggcgtcgt ccacgagtac
     1441 tcgaccgtcg atggtgtgcg cgaggatgtc gtcgacatcc tgctgaacat gaaaggcatc
     1501 gtgttcaagc tgcacaaccg cgatgaagtc acgctcaccc tgcgcaagga aggcgagggc
     1561 gttgtgatcg ccagtgacat cgagctgccg cacgatgtcg agatcgtcaa cccggaccac
     1621 gtgatcgctc atctgtcggg cggcggtcgc ctggaactgc aggtcaaggt tgaaaagggg
     1681 cgtggctacg tcccgggcaa cgtacgggca ttccgcgacg accattcgaa gaccatcggt
     1741 cgcatcgtca tggacgcgtc gttctcgccg gtcaagcgcg tgtcgtacgc ggtcgaagcc
     1801 gcccgtgtgg agcagcgcac cgacctcgac aaactggtga tgacgatcga gaccaatggc
     1861 gcgatgacgc cggaagaagc tgtacggcag tccgcgcgca tcctgatgga ccagctgcag
     1921 gtcttcggcg ccctcgaggg catggccgag acatccgccg cggcatcgtc gcgctcgccg
     1981 caggtcgatc cgatcctgct gcgtccggtc gatgacctgg aactcacggt gcgctcggcc
     2041 aactgcctga aggccgagaa catctattac atcggcgatc tgatccagcg cacggaaaat
     2101 gaactgctga agaccccgaa cctgggccgc aaatcgctca acgagatcaa ggaagtgctc
     2161 gccgcccgcg gtcttaccct tggcatgaaa ctggaaaact ggccgcctgc gggattggac
     2221 aagtaagaag accgcggcga cgcgcaagca tcgcggactc taaaccgacc cgcccgcaag
     2281 ggatcgaaga gtcggatcaa caaccgcccg gattgcgggc ggttaaagac tgaaaggaag
     2341 aacaatgcgt cacggacacg gactgcggaa acttaaccgc accagcagcc atcgactggc
     2401 gatgttgcgc aacatgacca actcgctgct gcgtcacgaa gccatcaaga cgacggtgcc
     2461 gaaggccaag gagctgcggc gcgttgtcga gccgctgatc acgctggggc gtgaaccgac
     2521 ggtggccaac aagcgcctcg cgttcaatcg cctgcgcgac cgtgagatgg tcgtgaagct
     2581 gttcaatgag atcgggccgc gctacaagac ccgtaacggc ggctacacgc ggatcctgaa
     2641 aatgggcttc cgcgtcggcg acaacgcgcc catggcgttc atggagctgg tcgatcgacc
     2701 ggcgccgctc gtcaaggaag agtcggccga cgcgagctga acatcgtgct gagctagact
     2761 gcaaaggcct gccatttggc aggccttttg cttttcgggg cctgtttttt tcgaccatgg
     2821 acgctgctgt cgtgcccgcg ctgaatctcg acgaggtgct cgatgtccgc gacctgcgca
     2881 aggtctacgg cggcgcgccg gtggtcgacg ggctgtcctt ctcgatccgt cgcggcgagt
     2941 gctacgcgct gctcggtccc aacggcgctg gcaagacaac gacgctgcgc tgctgcctcg
     3001 ggctgactcg gcccgatggc ggcacgatcc gactggtggg cgaaccggta ccgcgcgagg
     3061 cgcggcgtgc ccgcatccgc gtcggcgttg tcccgcagat cgacaatctg gacccggatt
     3121 tcactgtcac cgagaacctt gtggtctacg gtcgctactt cggactgacg gaccgcgacg
     3181 tcgcgcagcg gttgcctgcg ctgctcgaat tcgcaagcct tgcccacaag aaggatgcgc
     3241 ggatcggtga actgtcgggt ggcatgaagc gccgcctgac gctggctcgt tcgctggtga
     3301 atgacccgga cctgatcttc atggacgagc cgaccaccgg cctcgacccg caggcgcgcc
     3361 acctgatctg ggatcgcctg aagcagctgc ttgggcgtgg caagacgatt ttgctgacga
     3421 cccacttcat ggacgaagcc gaacgtcttg caaaccggct tgcggtgatc gaccacggcc
     3481 ggatgatcgc ggagggcgcg ccgcgagacc tcattcacga gcacattgag ccgcaggtcg
     3541 tcgagatcta tggtgacggt gctcggcaat gggcggaaca cgttggtcgc gcgctgctcg
     3601 gtcggctgga gatcaccggt gaaaccgtgt tctgctattc gcacgatcct gcgccggtat
     3661 tgcatgcgct ggagtcgaac gtcggattgc ggtacctgca ccggcccgcc aatctcgagg
     3721 acctgtttct caaactgacg ggccgcgaga tgcgcgacga gtcatgagtg cctcagtgcc
     3781 ggccacttca accggcagct cggtgtgggc gccgccgcgg ctgtcctggc gcttcgttcc
     3841 tgtgtggcgg cgcaattttc tggtctggcg caagctcttc gtcgagcggc tgttgaccaa
     3901 cgtggtcgag cccctgctga ccatcgcggc attcggctac gggcttggaa gcctgctgcc
     3961 agaggtcgac ggcgtgtcgt acctgcagtt tctcgccagc ggctcgattg cgatgagcgt
     4021 gatgtattcg gcgaagttcg agtcgctctg gggagcgttc tcgcgcatga gtatccagaa
     4081 gacgtgggac gcgatactgc acacaccgct cgcggtcgac gacctgctgc tcggcgagct
     4141 cgtgtgggcg gccagcaagg cgaccttcac cgccgtgtgc atcctcgcga tgatctggct
     4201 gctcgacatc gcgcgcgagc ccttgtccct gctcgtgctg ccgacggcgt tcttcattgc
     4261 gctggtgttc gctgcgatgg ccctgatcgt caacgcggtc gcgcgcagct acgatctgct
     4321 gtcgaactac ttcaccgtgg tcgtcatgcc gctcgttttc ctctccggcg tctactttcc
     4381 gctgacgcag ctgccgaact ggctgcgcac cctcgccgag tggctcccga tgacggcggc
     4441 cgtcaacctg gtgcgcccgc tggcactggg tcgatggccg acgaatggac tgagcgatgt
     4501 gctgctgctg gcgctctggg ggatcgcctg ctacacggtg gcattgggac tgacgcgcaa
     4561 gcgactgctc aagtgatcga caacgttctc atcgtcctga ccaactgccc tgatgtcggg
     4621 gtggccgacc gcatcgctcg cacattggtc gagcagcgcc tggtcgcgtg cgcaaaccgg
     4681 cttgcggagg tcgattcgat ctttcgctgg cagggtgctg tggagcgtgc ggtcgaagtg
     4741 ccattgctga tcaaaacgac ccgggaacgc tacagcgaag tcgaggcggc catacgcgcg
     4801 ctgcatccgt atgacgttcc cgagatcatc gcgtggccgg tcaccgccgg cctgccggcg
     4861 tatctgcgct gggtggtcga ggaaacgcag ccgcccctac aggcctgagg tgcgagcggt
     4921 gtcggcgtcg atgaacaaac cgtcatgccg gctcgagctg atgctccgag gtcgctagaa
     4981 ctttccaatg gccgccagcg atacgctcga cgcgatccgg ttcgacaagt ggctctgggc
     5041 tgcgcggttc ttcaagacgc ggtcgctggc ctccgaagcg gtcgaacgcg gccaggtcag
     5101 ggtcgacgac caacgcatca agccggcccg aacggcgcgc gtcggcgatc gggtcgtgat
     5161 ccagcggggt gacgatcgca tcgagttcgt cgtccgtgca ctgtccgcgg tgcgcggacc
     5221 cgcgcctgtg gcacagcgct tgtatgacga gacgccggaa agccgcacac gacgtgagca
     5281 ggcgtccgtc cagcgacgcc tggcgcgcga tcccgcggcg atgatcaagg gtcggccgtc
     5341 gaagcgcgat gcgcgggcac tgcgacggat gaccggcacg ccggacatgg accgatagat
     5401 cgaagggcga gaccgaatga aactccggat tccggtcgca ttgacctgca tgctcatggc
     5461 acctttgcat gcgtcggcta ttccgccgag tgttccgatg tcgggcttcg cgaagtacga
     5521 gttcaacaag tggctgttcc gctcctgcac cggctcaggg aagacatcga cgctgcaggc
     5581 gcagggcctg ccgttcatcg atgccacgcc cgaacgagtt ctcttcgcgg cgatccagca
     5641 gcgttggaag caatcggccg atccgctgcg tggcgtctat ctggagttcg ccgggttcac
     5701 cgagaatggg tgcgtgaccg ccactgacat gcaacgctcg ctgggatggg tcgaatcgtg
     5761 cgcgcagcgg ccgagcaata ttgcgcccgg agcgcgactg tgggcggctg gcaacgagcc
     5821 gggctggaat ttcgtggtcg atggcaagac cgcaaagttc cggacgccgg atgccgtgct
     5881 gaactttcct gtcgtggcag cgaagtcgtc ggacggaacg gtcacctatg tggcggatag
     5941 tgccggcgat cgcatccgcg tggagctgac ccacggcctg tgcaacgaca cgatgagcga
     6001 ggccgccttc ggccgccggg ccgtcgtggc cgtgcgcggc gcgctttaca cgggttgcgg
     6061 gctcgtgcgc tgatcagcgc agccacctcg gcggacgcgg cgtgcgtggc cgatcgagga
     6121 tgcggcgcgc ccagaagtag agcagcatca cgacgatgat ggatcccatg ccggtgatcg
     6181 tcagccagaa accgttaagt tcctgcagca aaggcatgtg ctggaagttc atgccgaata
     6241 ccccggtgat cagggtcagc ggcgcaaata tggctgcgat cgccgtgagc ttgcgcacga
     6301 tctcgttggt ctgatgcgtg ttggccgaaa agtgcagctg cactgcggat tcggccgaag
     6361 tctcgagccg gcgtgcgtga tgcagcacac gttggatgtg ttccagcacg tcgttgaggc
     6421 gcaccaggta cgcatcgttg atcgccgcgt ccggcgtttc ctcgaggtag gaatcgcgca
     6481 attccgtcag cgcgtcgtac tggccttcac acaggttctc cagcttgcgc agttcgatgc
     6541 gggcctcgag cagtgccgac cagttgctaa agggtcggcg cgggtccagc aggtcgcgct
     6601 gccagcgatc gagtcgatcc gacagtggct cgcgcaactc gaggtagcga tcgaccatgc
     6661 cgttgagcag ccgcatcatc agttcgcccg gcgacttggg cagtcgtgac ttgtcgtatg
     6721 gtgagggatc cgtgcgcatg cggggatcga cgagacgctg gcgcacctgc tcaaccgtct
     6781 tgctgctgcc ctggcgtacc gtgaccagca ggcgttcaag gaggaagaac gtgatcggtc
     6841 acgtgacgat ctcctgcagc atgcggcgtt ggtcggctgg tccggcccgt tcctcgagcg
     6901 gccggaactc tcctgtggcc agcttgcgga acacaagcat gtcgtagtct ttcgtactat
     6961 cgaagtacga cgggtgctgc aggttggccg cgtcctgcag atgcagatcg tgcagccgca
     7021 cggagaagat gcgctggacc gcctcgcgca gggcctcggg atcggcgatg gcctcgtcgt
     7081 gcagcacgtc cagccaaagg aagccctcgg ccggcgggcg cgccggctcg gcagcgagtt
     7141 ccgtcgcatg ctgcggtccg atctcgatcc acttcacggc agcggcttca gcgttcggcc
     7201 ttcaaccagc gggcgacggg cagggcgaag taggtcaaca cgccatctgc gccggcgcgc
     7261 ttgaatgcga gcagggattc cagcacgact ttcttctcat ccagccagcc gttggcggcg
     7321 gccgccttga gcatcgcata ttcgccgctc acctgatcga cgaacgtcgg tgcgcggtag
     7381 gcgtccttca cgcgccgaac gacgtcgagg taaggcatgc cgggcttgac catcaccatg
     7441 tcggcaccct ccgcgaggtc caggcctact tcccagagag cctcgtcggt gttggccggg
     7501 tccatctggt agaccatctt gttcgatttg ccgaggttcg atgtcgagcc aacagcgtcg
     7561 cggaaggggc cgtagaaggc gctggcgtac ttggcggagt acgccatgat ccgcgtgtgt
     7621 atgtgtcccg ctccttccag cgcctgtcgg atcgccccga tacgtccgtc catcatgtcg
     7681 ctgggcgcaa cgatgtccac tccggcggcc gcctggacca gcgcctgctg tacgaggacc
     7741 tcgatggtct cgtcgttcag tacgtagccc gcatcgtcga tgaggccgtc ctggccgtgc
     7801 gtggtgaagg ggtcgagcgc cacgtcggtc agcaggccga gttgcgggaa atgctgcttg
     7861 agcgtccgca ccgcgcgggg gaccaaccct tcggcgttcg ccgcctcggc accgttcgcc
     7921 gacttcagtg aggcatcgat gacggggaac agcgcaatta ccgggattcc gagccgaaca
     7981 cactcgtcgg caacgggaag cagctcgtcg atcgaaacgc gttcgatacc gggcatcgag
     8041 gccacggctt cgcgtcggcg tgcgccgtcg aggacgaaca ccgggtagat gaggtcatcg
     8101 accgtgagcg cgttctcgcg catcaggcgc cgggaaaagt cgtcgcggcg catgcggcgc
     8161 atgcgcacgg cagggaactg gccgaggatc tgcatgaggg gaagtcccaa ctcgatgatg
     8221 tgaactgcgt tcgctatttt gcgtctgagg tgatggacga tccgtcgtct cccgcttcac
     8281 ctccctgagc gggtgccttg aagactcaag gcattgcggc cccggacttg ttccggggca
     8341 tttttttcgc agtcatgata cggcgagtcg acgcgggtac cggctatgcc gagagccagc
     8401 gcgtcagggt ctcggtcaga tcgtcgatgc cctcgcgctt caggccggag aagagttgcg
     8461 cgctggcgcg cgctccgatg gcctcggcgc gtcgctgggc ggcggcaagc gccgcgcgct
     8521 tttccatcgt gcccagctgg tccgacttgt tcaacaggaa gtgcagcagg cagccgctgc
     8581 ggtccacgaa ccgcagcagg gcatcgtctc ccaccatgaa cggccgccgc gcatccatta
     8641 ccagaacgat gccgatcagc gtctggcgtt cgcgcacgta gctgccgacc agcaagtccc
     8701 acgtggcacg ctggccggcg tcggccttgg catagccata gccgggcaga tcgaccagga
     8761 tcccgcggtc gactcggtcg ccgtgctcgc ccttttcgct cagcgaaaag aagttgagga
     8821 gctgtgtgcg ccccggtgtc ttgctcgcaa aggccagtcg cttctgttgc gtcagtacat
     8881 tgatcaccgt cgacttgcca gcgttcgaac ggccggcgaa cgcaatttcc ggcaggctcg
     8941 gcggcgcgat tgccgtcagg tcggcaaccg aagtgacgaa ccgggcagag tgaaacaggg
     9001 acaaggcgac ggcaggagta gcgaaggact tcggtagaat acgcggcttt gcggagcggc
     9061 cagaagacca ctggccgcgc aggcgtggcg cgtgtgtgcc gcgcgcgcgg cggggccgcg
     9121 acatattcaa ccctcgaagg catgcgatgg aaatgtcgat gtggcggcgt tgcgggatcg
     9181 tgctgatggc aacgacgggg atggcgtggg cgccgacagt attcgcgcag gcgaagtcag
     9241 tgaaggccga tgcggcgaag ggccagcaga tcgcgtcaca ggtctgtgcc gcttgccaca
     9301 ccgcggatgg caattcgacg attgcggcga atccgaagct ggcgcagcag catacgagct
     9361 acctgcacaa gcaactggtg gattacacgg tgcgcgccgg ggagcagaag cctgcacgcg
     9421 agaacgccat catgaacggc atggcggcca cgctgtcgga ccaggacaag cgcgatgttt
     9481 cggcctggta tgccagccaa cccgccaagc cgggcacggc tcgcaacaag gacacgctcg
     9541 aactgggcca gcgcatctgg cgcgctggca tcccggagaa ggccttgccg gcctgctcgg
     9601 gttgtcacaa cccggctggg gcaggcattc cgaatcagta cccgcgtctc gcggggcaac
     9661 atgccgagta caccgaggcc acgcttaagt cattccgcga aggcacccgg cgcaacaacc
     9721 tgccgatgca gcagattgcc gcccgcctgt cggacgccga agtgcgggct gtcgccgact
     9781 acatccaggg gctgcgcaag taagcgttgc caaccgcagg gtgcgaggcg gccggtgcat
     9841 caagtcgtgc cgaagacctc gttgacctgc tgcgtgacgc gcacgaagct ggtccgttta
     9901 gagaactcct ttagccggcg cgcgccgaca taggtacacg cggagcgcac gccaccgagg
     9961 atttcctgtg ccgtggaggc taccggcccg cggaacggca ccagcacttc cttgccttcc
    10021 gccgcccggt acccggccac gccccccgca tagcgttcca tcgcggcctt gctgctcatg
    10081 ccgtaaaagc gctggtagct ggctccgtca cgttcgagcc gttccccgcc ggattcctcg
    10141 tgtcctgcaa acatgccacc gagcatgacg aagtccgctc ctccgccaaa ggccttggcc
    10201 acatcgccgg gcgtagtgca cccaccgtcc gaacagatca ggcccgacag accgtgcgcg
    10261 gcgtccgcgc actcgatgac cgcggacaac tgcgggtaac cgacgccggt catgcggcgg
    10321 gtggtgcaga ccgatccggg cccgataccc accttgacga tgtcggcgcc ggacaggatc
    10381 agttcttccg tcatctcgcc ggtcacgaca ttgccggcca tgatgacgag ccgcggatac
    10441 gctgtccgga tgcgggtgac gaacgtgacg aagttctcgt ggtaaccgtt ggcgacgtcg
    10501 atgcagacgt actcgaccgc atcggcggcc gcatccatca cgcgcctgaa tttctcctcg
    10561 tccaccttgg tgatgcccat cgaatagaag gcggccgact tgcgctccag cgagcgaaag
    10621 aacgcaacgc agtcgtcgac gctgtagtgc ttgtgcagtg ctgtcgacag ccggaaggtg
    10681 tcgagggcgc gtgccatctc gaacgtgccg atcgtgtcca tgttcgacgc gatgatgggg
    10741 atgccgtgat acttgacgcc ggcatgtcga aacgtgaact cgcgcgagat gtcgacatcc
    10801 gaccgggacg tcagcgtcga gcgcttgggg cggatcagga cgtccttgaa gtccagcttg
    10861 acctggtaat cgatgtgcat cgggggcctg caagacggtg gaccggcttc agtctagcgt
    10921 tcgaaactcc cgctgcgtcg cgctgtcaga ttggaattcc cgttagccca ggtccgagcc
    10981 tgtaaggatc ggctgggttg ggacgaccaa gcggtgcatc cttgagaggt ttcatgttga
    11041 tccgacatgc actgccgttt agtgcctccg aaatcactcc cgaaggcgtg gttcgccaac
    11101 ggcgcgaatt cctgcaggtg ggagttggcg gcgcgtttgc cctgatggca ccctcgctcg
    11161 caaacgcaca ggtcccgtcg ccggcagcgc ttgcggcacg acccagtccc ctctcggtga
    11221 tggaaaagcc aaccccggcc aacctcgtca cgagctactg caacttctac gagttcggga
    11281 ccgagaagga cgatccgccg caacgcgcac cgcaatcgct gaagacccga ccctggaccg
    11341 ttcgagtcga gggcgaggtc aggaaagcgc gcaccttcga catcgatgac ctgctggcgc
    11401 ttgcaccgat ggaggagcgc gtctatcggt tgcgctgcgt cgaggcgtgg tcggccgtcg
    11461 ttccctggat tggctatcag ctggccgaac tgatccggcg ggtcgagccg accgggaacg
    11521 cgaagtttgt ggagttcacg acgctggcgg atccgaagca gatgccgggc ttgcgctcgt
    11581 cagtgatcga ctggccctac accgaaggac tgcggctcga cgaggcgatg catccgttga
    11641 ccctgctcgg cttcggcatg tacggccaga ccatgccggc gcagaacggc gcgccggtac
    11701 gtctggtcgt tccatggaag tacgggttca agagcgcaaa gtcgatcgtg ctcatccgtt
    11761 tcaccgagaa gcaaccgatg atcgcgtggc agaaatccaa cccggccgag tacggtttct
    11821 actcaaacgt gaatccggaa cgcgatcatc cgcgctggag ccagaaaagc gaacgtcgcc
    11881 tgggcgagga aatcttcagc cggcgtcgcc ccaccttgat gttcaacggc tatggggagc
    11941 aggtcgcctc gctgtatgcc gggatggacc tgaagaagtt ctattgatgg ccgggctgtc
    12001 gcgcgcattc cgctttgtac cgcccgctct gccggcgtcg gtgttcgccg ccgtgcgcgc
    12061 ggttgtcttc acgctggcga tgctgccgct gttggcgctg atctaccgcg cgactgacgg
    12121 cgggctgggt gccaatccgg tcgaggccat tctgcggtcg ctcggaacct ggacgctggt
    12181 tctgctgctg gtaacgctgg ccatcacgcc gctgcggtgg ctgaccggtt gggcgtggct
    12241 gttgcggctg cggcggatgc tgggcctgac ttcattcttc tatgcgctgc tgcatgtggt
    12301 ggccttcgtc tggctggatc actggttcga gtgggccgcg atcgttgccg atgtagtgaa
    12361 gcggccgtac ctgacgttcg gcttcgcggc attcgtgttg atgattcccc tcgcggcaac
    12421 ctcgaccaac gccatggtgc gccgcctcgg cggccggcag tggcagcagt tacaccgtct
    12481 cgtctacggc atcgccgtac tcggagtgct gcatttctgg ttccacaagt tggccaagaa
    12541 cgaccttgcg gagccggcgc tctatgcagc cgtgctcgcc gtcctgctgg gcacgcgcct
    12601 gatccactgg tgggtcgcct cgcgacgacc cggtggcctc gttcccgccg tcagacgcta
    12661 aaggccccga cgccggtcgt tcccgggcgc ctgcagaggg taggccgcct cgttgtggac
    12721 cgtctccgcc tagggcaacg ttgactcgcc tgcaaacaac tcggcgacgg tttcgcgacg
    12781 gcgaacggcg aaggcgcggt cgccgtcgac catgacctcg gctgcacgcg gccgcgtgtt
    12841 gtagttcgaa ctcatcgcca tgccataggc acccgctccg gcgatggcca gcagatcacc
    12901 cggcgcaagt gacagcgcac gttctcgcgc cagccagtct cccgattcgc agataggtcc
    12961 cacgatgtcg taggtctgcg tcggtccaga acgggcctcc accgccacga cgtccatcca
    13021 ggcgtcatat agggtcgggc gcagcagatc gttcatggcc gcgtccacga tcgcgaagtt
    13081 ctttgccgcg ccgtgcttca ggtactccac gcgcgttacc agcatgccag catggccgac
    13141 cagcgatcga ccgggctcga ccatcaccgt cttggctccg aagccgcgcg cgtcgacccg
    13201 ctgcagcagc gcgcggacca gctcgccgct cgcggggggc gactcctgcc gatagcgaat
    13261 gccgagaccg ccgccgaaat cgatatggcg cagtccgatg ccgtcgcgct ccagcgcttc
    13321 gacgacatcg agcacgcggt cggcagcatc gaggtaaggg gccaactccg tgacctgaga
    13381 gccaatgtgg cagtcgatgc cgaccacctc gagtccagaa cgtcgcgctg cgtcccggta
    13441 cagcggcagc gtctcgtcga tgtctattcc gaacttgttg tccttgaggc ctgtggcgat
    13501 gtaaggatgc gtcccggcat cgacgtccgg attgacgcgc agcgagatgc gggcacggcg
    13561 tcccaggtgg caagcgacgg catcgatgcg ctccagttcg gccgcagatt cgacgttgaa
    13621 gcacaggatg tcgacggcca gcgcctgctc gatttcggct tcgctcttgc ccacgccgga
    13681 aaagacgact ttgctggccg cgccgcccgc cgccagcact cgcgcaagtt caccgcccga
    13741 gacgatgtcg aagccagcac ccagacgagc cagcacgttc agcacggcaa ggttggagtt
    13801 cgccttcatc gcgtagcaga ccagcgacgc gcgtcccgcc aaagcctctg cgtaggatac
    13861 gtagg
//
